LOCUS       DAVLVZ010000001        80647 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000001 DAVLVZ010000000
VERSION     DAVLVZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 80647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.001"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..439)
                     /locus_tag="VYN77_000001"
     CDS             complement(<1..439)
                     /locus_tag="VYN77_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1557548.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTKIKRRTVRKCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSVTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKA"
     gene            complement(884..1282)
                     /locus_tag="VYN77_000002"
     CDS             complement(884..1282)
                     /locus_tag="VYN77_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1557549.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(1610..2092)
                     /gene="greA"
                     /locus_tag="VYN77_000003"
     CDS             complement(1610..2092)
                     /gene="greA"
                     /locus_tag="VYN77_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1557550.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(2163..3818)
                     /gene="mltG"
                     /locus_tag="VYN77_000004"
     CDS             complement(2163..3818)
                     /gene="mltG"
                     /locus_tag="VYN77_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1557551.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(3899..4393)
                     /locus_tag="VYN77_000005"
     CDS             complement(3899..4393)
                     /locus_tag="VYN77_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1557552.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(4390..4854)
                     /locus_tag="VYN77_000006"
     CDS             complement(4390..4854)
                     /locus_tag="VYN77_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1557553.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(4864..6198)
                     /locus_tag="VYN77_000007"
     CDS             complement(4864..6198)
                     /locus_tag="VYN77_000007"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1557554.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(6209..6826)
                     /locus_tag="VYN77_000008"
     CDS             complement(6209..6826)
                     /locus_tag="VYN77_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1557555.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(6874..9972)
                     /locus_tag="VYN77_000009"
     CDS             complement(6874..9972)
                     /locus_tag="VYN77_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1557556.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(10065..11231)
                     /locus_tag="VYN77_000010"
     CDS             complement(10065..11231)
                     /locus_tag="VYN77_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1557557.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNKSKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(11239..12333)
                     /locus_tag="VYN77_000011"
     CDS             complement(11239..12333)
                     /locus_tag="VYN77_000011"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1557558.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLATIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKLFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            complement(12490..14172)
                     /locus_tag="VYN77_000012"
                     /pseudo
     CDS             complement(12490..14172)
                     /locus_tag="VYN77_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(14344..16302)
                     /locus_tag="VYN77_000013"
     CDS             complement(14344..16302)
                     /locus_tag="VYN77_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1557559.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGIESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(16506..18128)
                     /locus_tag="VYN77_000014"
     CDS             complement(16506..18128)
                     /locus_tag="VYN77_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1557560.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            18234..19679
                     /locus_tag="VYN77_000015"
     CDS             18234..19679
                     /locus_tag="VYN77_000015"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1557561.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(19722..19919)
                     /locus_tag="VYN77_000016"
     CDS             complement(19722..19919)
                     /locus_tag="VYN77_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1557562.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKVKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(20020..20628)
                     /locus_tag="VYN77_000017"
     CDS             complement(20020..20628)
                     /locus_tag="VYN77_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV1557563.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            complement(20653..21165)
                     /locus_tag="VYN77_000018"
     CDS             complement(20653..21165)
                     /locus_tag="VYN77_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1557564.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAAFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(21251..22186)
                     /locus_tag="VYN77_000019"
     CDS             complement(21251..22186)
                     /locus_tag="VYN77_000019"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1557565.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEYGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(22257..22556)
                     /locus_tag="VYN77_000020"
     CDS             complement(22257..22556)
                     /locus_tag="VYN77_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1557566.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(22546..23295)
                     /locus_tag="VYN77_000021"
     CDS             complement(22546..23295)
                     /locus_tag="VYN77_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1557567.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(23349..23708)
                     /locus_tag="VYN77_000022"
     CDS             complement(23349..23708)
                     /locus_tag="VYN77_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1557568.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(23710..25110)
                     /locus_tag="VYN77_000023"
     CDS             complement(23710..25110)
                     /locus_tag="VYN77_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1557569.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSIRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTIFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(25405..25644)
                     /locus_tag="VYN77_000024"
     CDS             complement(25405..25644)
                     /locus_tag="VYN77_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1557570.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(25676..26146)
                     /gene="ssbA"
                     /locus_tag="VYN77_000025"
     CDS             complement(25676..26146)
                     /gene="ssbA"
                     /locus_tag="VYN77_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1557571.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(26158..26448)
                     /locus_tag="VYN77_000026"
     CDS             complement(26158..26448)
                     /locus_tag="VYN77_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1557572.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(26601..27947)
                     /gene="asnS"
                     /locus_tag="VYN77_000027"
     CDS             complement(26601..27947)
                     /gene="asnS"
                     /locus_tag="VYN77_000027"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1557573.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(27963..28328)
                     /locus_tag="VYN77_000028"
     CDS             complement(27963..28328)
                     /locus_tag="VYN77_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1557574.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            complement(28321..29508)
                     /locus_tag="VYN77_000029"
     CDS             complement(28321..29508)
                     /locus_tag="VYN77_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1557575.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(29505..29993)
                     /locus_tag="VYN77_000030"
     CDS             complement(29505..29993)
                     /locus_tag="VYN77_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1557576.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            30314..30862
                     /locus_tag="VYN77_000031"
     CDS             30314..30862
                     /locus_tag="VYN77_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1557577.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQSVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            complement(31100..36484)
                     /locus_tag="VYN77_000032"
     CDS             complement(31100..36484)
                     /locus_tag="VYN77_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1557578.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETNPAAT
                     TDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDVDRGYSG
                     FRAAPAPTNQPVTNLGNNTVFTDISRGSHTFRGNGNSRRGNPIHFDVTTTRVGNRVEF
                     SIAYSGPGEFVNNNFLLDKGNGFGEPSRATISTNRLRDQTKDIRKGANFVSHSSYSLT
                     SAVETNSNQTIRFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNYYFRE
                     KPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIYKVHQ
                     LGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFYLDND
                     GNGVYTEGVDELVQKIAIKDGAKGEKGVQGERGLTGAQGQAGRDGVTPTVTVKDNKND
                     GTHTITINDGRGNVASTVVRDGFDGASPLVATQRNEADKTTTVIFYYDQNGNNELDAS
                     DKKLKEVIIADGAKGEQGLQGRDGVQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDGK
                     SLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNGK
                     NGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTTT
                     IRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIKG
                     PKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQGK
                     DGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHSTD
                     ITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGKY
                     TPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPLA
                     VIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGAIGQDG
                     QTPTITTQRGQDGQSTVVTITTPGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRIR
                     RATDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITTK
                     DNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKET
                     VVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPDG
                     SHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETTV
                     RDGKSPTAKVVDNGDGTHTITVVNSDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVTG
                     ADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPTV
                     TVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGTV
                     TKTVIKDGKDGRDGRDGRDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVKP
                     VPAVPEQPVVPTPAQPATPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILL
                     SLYVGRRKED"
     gene            36700..38184
                     /locus_tag="VYN77_000033"
     CDS             36700..38184
                     /locus_tag="VYN77_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1557579.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELTQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            complement(38235..38963)
                     /gene="yaaA"
                     /locus_tag="VYN77_000034"
     CDS             complement(38235..38963)
                     /gene="yaaA"
                     /locus_tag="VYN77_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1557580.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(39042..39206)
                     /locus_tag="VYN77_000035"
     CDS             complement(39042..39206)
                     /locus_tag="VYN77_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1557581.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGIALV
                     LAAVWLGWPK"
     gene            complement(39385..40980)
                     /locus_tag="VYN77_000036"
     CDS             complement(39385..40980)
                     /locus_tag="VYN77_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557582.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVEGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            complement(40992..41402)
                     /locus_tag="VYN77_000037"
     CDS             complement(40992..41402)
                     /locus_tag="VYN77_000037"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1557583.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETTRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            complement(41518..42309)
                     /gene="yghU"
                     /locus_tag="VYN77_000038"
     CDS             complement(41518..42309)
                     /gene="yghU"
                     /locus_tag="VYN77_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1557584.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            complement(42322..45018)
                     /locus_tag="VYN77_000039"
     CDS             complement(42322..45018)
                     /locus_tag="VYN77_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1557585.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            45322..46506
                     /gene="mntE"
                     /locus_tag="VYN77_000040"
     CDS             45322..46506
                     /gene="mntE"
                     /locus_tag="VYN77_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1557586.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLENRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            complement(46649..48520)
                     /locus_tag="VYN77_000041"
     CDS             complement(46649..48520)
                     /locus_tag="VYN77_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1557587.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQN
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(48517..49701)
                     /locus_tag="VYN77_000042"
     CDS             complement(48517..49701)
                     /locus_tag="VYN77_000042"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1557588.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHNPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            complement(49713..50480)
                     /locus_tag="VYN77_000043"
     CDS             complement(49713..50480)
                     /locus_tag="VYN77_000043"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1557589.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            complement(50757..51605)
                     /locus_tag="VYN77_000044"
     CDS             complement(50757..51605)
                     /locus_tag="VYN77_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1557590.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            complement(51607..51981)
                     /locus_tag="VYN77_000045"
     CDS             complement(51607..51981)
                     /locus_tag="VYN77_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1557591.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            complement(52055..53407)
                     /locus_tag="VYN77_000046"
     CDS             complement(52055..53407)
                     /locus_tag="VYN77_000046"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1557592.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKKTGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            complement(53431..54210)
                     /locus_tag="VYN77_000047"
     CDS             complement(53431..54210)
                     /locus_tag="VYN77_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1557593.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            complement(54197..55054)
                     /locus_tag="VYN77_000048"
     CDS             complement(54197..55054)
                     /locus_tag="VYN77_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1557594.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERW
                     LGGWKHEKK"
     gene            55190..56158
                     /locus_tag="VYN77_000049"
     CDS             55190..56158
                     /locus_tag="VYN77_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1557595.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            complement(56192..57103)
                     /gene="whiA"
                     /locus_tag="VYN77_000050"
     CDS             complement(56192..57103)
                     /gene="whiA"
                     /locus_tag="VYN77_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1557596.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(57100..58077)
                     /locus_tag="VYN77_000051"
     CDS             complement(57100..58077)
                     /locus_tag="VYN77_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1557597.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(58074..58964)
                     /gene="rapZ"
                     /locus_tag="VYN77_000052"
     CDS             complement(58074..58964)
                     /gene="rapZ"
                     /locus_tag="VYN77_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1557598.1"
                     /translation="MTKKQFHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(59016..59396)
                     /locus_tag="VYN77_000053"
     CDS             complement(59016..59396)
                     /locus_tag="VYN77_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1557599.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(59407..59994)
                     /locus_tag="VYN77_000054"
     CDS             complement(59407..59994)
                     /locus_tag="VYN77_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1557600.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(60003..61235)
                     /gene="clpX"
                     /locus_tag="VYN77_000055"
     CDS             complement(60003..61235)
                     /gene="clpX"
                     /locus_tag="VYN77_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1557601.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(61267..61437)
                     /locus_tag="VYN77_000056"
     CDS             complement(61267..61437)
                     /locus_tag="VYN77_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557602.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            complement(61437..61943)
                     /locus_tag="VYN77_000057"
     CDS             complement(61437..61943)
                     /locus_tag="VYN77_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1557603.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(62073..62591)
                     /locus_tag="VYN77_000058"
     CDS             complement(62073..62591)
                     /locus_tag="VYN77_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1557604.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            complement(63088..64593)
                     /gene="lytC"
                     /locus_tag="VYN77_000059"
     CDS             complement(63088..64593)
                     /gene="lytC"
                     /locus_tag="VYN77_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV1557605.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMIQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            complement(64631..65389)
                     /locus_tag="VYN77_000060"
     CDS             complement(64631..65389)
                     /locus_tag="VYN77_000060"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1557606.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(65487..66164)
                     /locus_tag="VYN77_000061"
     CDS             complement(65487..66164)
                     /locus_tag="VYN77_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1557607.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLIKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            complement(66173..67117)
                     /locus_tag="VYN77_000062"
     CDS             complement(66173..67117)
                     /locus_tag="VYN77_000062"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1557608.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            complement(67299..67811)
                     /locus_tag="VYN77_000063"
     CDS             complement(67299..67811)
                     /locus_tag="VYN77_000063"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1557609.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(67898..68656)
                     /locus_tag="VYN77_000064"
     CDS             complement(67898..68656)
                     /locus_tag="VYN77_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1557610.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            68864..69010
                     /locus_tag="VYN77_000065"
     CDS             68864..69010
                     /locus_tag="VYN77_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557611.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            complement(69157..70287)
                     /gene="ugpC"
                     /locus_tag="VYN77_000066"
     CDS             complement(69157..70287)
                     /gene="ugpC"
                     /locus_tag="VYN77_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1557612.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(70570..70655)
                     /locus_tag="VYN77_000067"
     tRNA            complement(70570..70655)
                     /locus_tag="VYN77_000067"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70619..70621),aa:Leu,seq:aag)
     gene            complement(70737..71312)
                     /locus_tag="VYN77_000068"
     CDS             complement(70737..71312)
                     /locus_tag="VYN77_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1557613.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(71312..72100)
                     /gene="codY"
                     /locus_tag="VYN77_000069"
     CDS             complement(71312..72100)
                     /gene="codY"
                     /locus_tag="VYN77_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1557614.1"
                     /translation="MAHLVEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            complement(72364..73923)
                     /locus_tag="VYN77_000070"
     CDS             complement(72364..73923)
                     /locus_tag="VYN77_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1557615.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNTLSEKQ
                     TGFVIRNKGDK"
     gene            complement(74122..75348)
                     /locus_tag="VYN77_000071"
     CDS             complement(74122..75348)
                     /locus_tag="VYN77_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1557616.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILAGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            75542..76858
                     /locus_tag="VYN77_000072"
     CDS             75542..76858
                     /locus_tag="VYN77_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1557617.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            76957..78300
                     /locus_tag="VYN77_000073"
     CDS             76957..78300
                     /locus_tag="VYN77_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1557618.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            78300..79082
                     /locus_tag="VYN77_000074"
     CDS             78300..79082
                     /locus_tag="VYN77_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1557619.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            79208..80290
                     /locus_tag="VYN77_000075"
     CDS             79208..80290
                     /locus_tag="VYN77_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1557620.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTIFETAEFENLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     IIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            80283..80486
                     /locus_tag="VYN77_000076"
     CDS             80283..80486
                     /locus_tag="VYN77_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1557621.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
BASE COUNT        23121 a        18171 c        14912 g        24443 t
ORIGIN      
        1 gcgcttttcc ttttctaggg taaagcagat cctgtttgct taaccccaat tttccatgat
       61 gaatccaata gtaaatggtt gaaattccca cgttaacccc tttagccatc accatcattt
      121 caggcgaaaa tttttggtta tgatagtgga gaatcttttc ctttagttcc ttggtcacgc
      181 ttgatttctt gaccgagcgc ttgcgattgt tttcataaga ctgttgagca tagtcggcag
      241 aataaacctc tttgaagcgc ccttttccaa gacattttcg gactgtccta cgcttgattt
      301 tagtgtggat agtttgagga gcttttccaa gtagagaggc aatttctcta tttgattttc
      361 cttctttttt ccatctttcg attaagcgac ggctatcgat tgtcaaatgt ttaccttttg
      421 tagtataatt gtcttgcatt tctgtgcctt tcttgtgttt gtggttgaac aacaagtata
      481 acacagaggt gttttcttat gcctacaaga gctttcatta tttccatttt cttttggatt
      541 tcactctatt ctgaaaaact tgtgtatatt tactgaagct agcaagtctt acctgtaaat
      601 ttaatgaaag caacacaaaa tccgagaggg gaatctcgga ttaatagata gagagttttt
      661 agtttaaata aattgtttaa aatatcaaca acatcacttc ttttcttaac ctgataagtc
      721 ttgattccta attttggggc tacgattata ttgtcctcaa tatcgtctag aaagacacaa
      781 tttctaggtt ataactggta tttatcaata gttactcata tacatcagtc cacctccata
      841 cttatgtgcg agcctctctt tgtattatac ctccatactc accttacaga ttcttttggt
      901 aataatatct ttgcctaatg tagagatagt cttgcaaaga aaaaacttcc ttgtagccat
      961 gtttctgata aaagtccggt gcctggaact ggtaagtatt gacaaaggca aaacaacaat
     1021 ttcgattctt agcttcactt tctgcctgtt gcaatagttt tgaaccgatt ccttgccctc
     1081 gcagttcctc ttttacaaac aaatactcga tttctagcca atttccaaaa gtctctgcta
     1141 tcaaacctgc caggagattg cccttttcat cttcgacata aagattaagt ggctcacttt
     1201 cagcctcttc tctttttgaa cggttataaa cacgaatcag attccctatt tcttgcgatt
     1261 tatgtgattc cttattttcc aatctaaagt atagtgaaat gaaataaaac atgcgcaaat
     1321 cgattaagga atttaatcta atttctaaca atgtcttaga aatcaaagtg tactatttta
     1381 acttcaatgc actatacatc taatactcaa taaaaatcaa agagcaaact aggaaactag
     1441 ccgcaggttg ctcaaaacac tgttttgagg ttgtagatga aactgacgaa gtcagtaacc
     1501 atacatacgg caaggcgacg ctgacgtggt ttgaagagat tttcgaagag tagcaaaatg
     1561 gaaaaaggag tgagtgaagc acatcgcctc cccactcctt tttctgtttt taggctgttt
     1621 tttcaacctt caagattttt acatcatagc taccaacagg cgtttcaatg gttgctgtat
     1681 cacctgtttt cttgccaatc aaggcctgcc caattgggct ttcatttgaa accttacctg
     1741 caaaggcatc cgcaccagct gaacctacga taatataaac ttcttcttcg tcctcaccaa
     1801 tttcttggat ggtgactgtt ttaccaatcg ctacttcgtc ctgggcaact gcgtcgctat
     1861 tgacgatttc agcatagcgg atttttgttt ctaagctaga gatttgtcct tcgacaaagg
     1921 cttgttcatc cttagctgct tcgtactcac tgttttctga aaggtcaccg tatgaacggg
     1981 caatcttaat gcgttctacc acttctggtc gacgaaccaa tttcaattct tctaattctt
     2041 tttcaagttt ttccttttcc tcaagggtca taggatatgt tttttctgcc atttttctca
     2101 actttcttct gataatattt tctaaagaaa attatgtgaa gtatcacata attttagttt
     2161 gtttagttta atttgctgtt gacatgttca gcgacattgc ggtcgtggtc ttcttgattg
     2221 ttagcatagt agaccttgcc ttctgtgaca tctgctacaa agtagaggtt atcgctctta
     2281 gtttgattga tgcttgactc aatcgcatcc agacttggac tatcgactgg accaggcatg
     2341 agacctacat ttttataaac attataaggt gaatcaatgt tggtatcaat cgcaacatcc
     2401 tcagctagac tgatattttg ccccagtttt ccttgggcat acaagattgc aatattactt
     2461 tgaagtggca tatcacgatt caaacgattg tagaatacac ctgcaatgag cttacgatct
     2521 tctgtcttgg caccttcttt ttcgaccaag gaagcaatgg tcaacaactc attgacagtc
     2581 aagtttttag atttgatagt actatagtaa ggagataggt tcttatccat agcagctaac
     2641 atctcatcaa tcaagctctc aatagttgtg ctttccttga tagagtatgt agctgggaaa
     2701 aggtatcctt ccaaacgata acgcgcaccg ctgtctttta caggcaaact ttccagtaaa
     2761 gtaggatatt tcgctactgc ttgactgata aacgtctcat cttgaacttt agccaagaaa
     2821 gcctccgctg tcaaagactc tttgaagtca ccttgcaatt gacccacagt ttgagcaatc
     2881 tgatccaagg tataaccttc tggaattgtc aaagtcgcaa gtacaggttc ttgcggttca
     2941 tctgttccac ctttttgcaa ctctttgagt aagtcttctg tactcatact cttttgcaaa
     3001 ttgtagtaac ctgctttcaa gtcggtataa tttttatact tggcataaaa actaaaaatc
     3061 agaccatgct ttaccaaacc agctttttca agcgtcgtac cgatttcttg aacgtttgaa
     3121 ccttctggaa ttccaaccgt cacatatttc ttagaattag catcgatagg taataaggaa
     3181 tctagcacgt actggtaacc aaagtaacca cctgctgaga gcaaggcaag gaatacaagg
     3241 aatgaaatca caaaagcttt cacatgtgac ttcttctctt tcttaggctt aacgggtttt
     3301 gctccttctc tacggctacg acgtgatgta tctctgatga tatccactgt ttctgtagca
     3361 ggcgtaggtg tttctggacc tgcttgttct gcttttttct ctgccgtcgt ataggaaaca
     3421 gctacccttg ttggggtttc attgtattct ctttcaagtt tcttaggtct aacaggacct
     3481 ggacctggtc ttgatccact ttcttccgct ggagaagaag gtacatcttg acttggatga
     3541 cttggaacac caggagtttc tctttgaatc tcatctgctg gagaagctgg tacaccttga
     3601 cttgggtgag taggcacggt aggagctttt ctcataatct cctctaccgt tgacaaggaa
     3661 tcagccatga gttcttcagc tgaaggttca tttgcaggag tgcgaactac tgcctcatct
     3721 tctttcagaa cttcatcata gccttttact ttttctaaat ctctcagaat ctgctcttta
     3781 aagcttaatt tctcttcttc tcttgacttt tcactcaaaa gtttttcctc cttgttgata
     3841 atccataata ttatatctta aaagtccaaa aaaagcaatc tatgatactt ttcctaactt
     3901 attttttcat ttcccagacc atatcatacc atgtttcccc tgcaaaggtt gactgggact
     3961 ggccttcgtt gacaaatcca tgtttttcat aataggcgat gagatagtca tgacaggtta
     4021 gattaatgcc atctctctca tcttcaagag caacttcttt caaggctgtc agcaatttct
     4081 gacccagtcc gagtccctgt gcctccttgg caatagacag acaggtcaca gagatataac
     4141 caccaggctc atgactatag tcttttattt cttctgtaaa agactggtct tgcagatggc
     4201 ggtgcaggcc aactggtcct tcgatataac ccatgattct tccttctttt tcagcaacca
     4261 gaaaagaggt ctgaatttct ctcaaatgtg cttcaaagac agaaggagga atggcttctt
     4321 cgaccgaaaa attttcaaat tcaagttcaa caatccgatc caaatcttct aatcttgctt
     4381 gtctgatttt cattgttcct ccagataaaa gggattaaac caaatcatac tatagccctg
     4441 gctagttgca tagagcaaag tttcttcttc atcaacaaaa ccgttcattt caaaatagga
     4501 aagcagctca tcaggactct ccaaacgaat ccctttgtaa tccagctcaa ctgccacctc
     4561 tttcaaggct gcaagaagaa gtgttcccag gccctgtctc tgatggtcag actcgatgac
     4621 taaagaatgt acttttagac attgcggatt gtctgactgg ggacttgata aaatatagcc
     4681 taaaagttga ttttcatccc tagctagaag aaaggtatcc gcacacttac ggatactttc
     4741 ttctaaaata tgggaaagtt gctgcttttc agctggaaaa gacgaggtct gaagtgcccc
     4801 tatctcaggc aaatcaaact tgcttgcctg aatgatctta attggaattt ccatgggaac
     4861 atcctattga acattgcttg tcaagttaga caagagacgc tcaaatgagt attcataggt
     4921 ttggatgtct cctgctccca taaagacgta aacagcattg tcatggtcta ggagtggaga
     4981 aacattttca acagtaatca cttggtgttt tttgttgatt ttattggcta ggtcttctac
     5041 cttaacgtca ccatgatcta cttcacgagc cgagccataa atttgcgcta gataaacagc
     5101 atctgcttgg tttaaagcat gggcaaagtc gtccaacagg gcaatggttc ttgtaaaggt
     5161 atgcggttga aagactgcta caatttcctt gcttgggtat ttctgacgag ccgcatccaa
     5221 ggtcgcaata atttctgttg gatgatgggc aaagtcatca ataatcactg tatcattgac
     5281 aattttctca gtgaaacgac gtttaacacc ggcaaatgtt ttcaagtgct cacgcaccaa
     5341 gttcaaatca aatcctgctg tgtaaagaag accaataacg gctgtcgcat tcatgatatt
     5401 gtgacgacca aaggttggaa tgtggaattg ccccaagttt tgtccacgga aatgaacggt
     5461 gaaggttgaa ccagttgttg aacgaagaag atcactagct acaaagtcat tgccttcagc
     5521 ttcaaaacca taataataaa ttggtgcatc agacgtaatc ttacgcaatt cagcatcttc
     5581 accatagaca aaaagaccct tggtaatttg tttggcatag tcgttaaagg cattgaaaac
     5641 atcctcgaga cttgtgaaat aatctggatg gtcaaagtca atgttggtga taatagagta
     5701 ttctgggtgg taaggcatga agtgacgctc atattcgtca gattcaaaga caaaatattt
     5761 ggcattagcc gaaccacgac ctgtcccatc tccaatcaag aagctggtat ctgtaatgtg
     5821 agacaagaca tgagacaaca tacctgtcgt tgaagttttt ccatgtgctc ctgctactcc
     5881 catgctaaca aagtcacgca taaagctacc tagaaactca tggtaacgtt tgtagctgat
     5941 accattttgg tccgcatagg caatttcgac gttgttatct ggacgaaagg catttccagc
     6001 gataatttcc atatcaccgt ctagattctt ttcatcaaaa ggaagaatgg taattcctgc
     6061 ctgctcaaga ccgcgttggg taaagtagta cttttcaaca tctgatccct gaaccttgtg
     6121 ccccatctgg tgcaacatca aggccaaggc actcatccct gatcccttaa ttccgataaa
     6181 atgatatgtc tttgacatgt tttctcccct attctgtcat tctggtcaga ttcaactctt
     6241 gggcaacccg acgttcttgt tctgtttgtt tacttttttt attgtagatt tggctcttct
     6301 ttagaaaatc ataattgttt ttctttggag caggtgctga cacttcttca ttcttggtag
     6361 ggatagaatg aacttcttcc gccaagatat aatgagactg ggtcaatttt tggctatatt
     6421 tgacaaattc accaggattt tccttttgga aaggagttgt cggttgattg ccctgtctaa
     6481 ctagactggg ctgagaatga cgtctcgcaa ggctgaaatc ctgagttagg tagttagcag
     6541 agcgtttctt tttcaagtcc gcacgcgctt cttcacgcgc cacctccgca tagctctttc
     6601 cttctttttt aacccctaaa ggagcctttt taggtttttc gacttgcttt tcaatcggtt
     6661 ttactggttt ttcttcagta ataggagccc attctaaata atttttatct cgatactcac
     6721 ccttgatatt actgatcaga tcagactcat catagagatt catgactggc atttcagtca
     6781 acatgacctc gtcatctgac accaatggaa atcgttcttg tttcattttc tatttccttt
     6841 caacacttca ttatagcgta ttgtcttgat ttttcaagtg ctggcttcag aaattcccaa
     6901 aatttctcta atttctgcta gggtcagact accacgtgac tctgtgccat ccaatacttg
     6961 tgacaccaga tgtttctttt gttcttggag ttcctgaatt ttttcttcaa tggttccctt
     7021 ggtcaccaag cgatagacct caaccgtttc ttcctgaccc atccgatggg cacggccaat
     7081 ggcttgcgct tccaccgcag gattccacca aaggtcaacc aagatcactg tatctgcacc
     7141 tgtcaggttc agaccgaccc caccagcctt gagggaaatc agaaaggcat ctctttctcc
     7201 ttggttaaag gccttggtca tgtcttgtct ttccttggct ggggttgaac ccgtaatttt
     7261 aaaggatgtc aggcccaagt ctggcagttc ttgttcaatt ttttccaaca ttcccttgaa
     7321 ctgagagaaa atcaagacac ggtgtccgcc gtctgccacc tgtaccagta gatctcggag
     7381 actatctagt ttgccgctgg ctccctgata atcttccata aacagggcag gagtgtcaca
     7441 tatttgacgc aagcgcatca aaccagataa aatttccaca cgacttcgct gaaattcctg
     7501 ttctgacact tgagccagat ggtctcgcat ctgttgtaac tgggcaaggt aaatagcctt
     7561 ttgctggtct tccagttcat ttttataaac cacctcaatc aagtctggca attcagtcag
     7621 aacttcttct ttcttgcgtc gcatcacgaa aggcttgata aactgagcca ctcgctctgc
     7681 tggcaatttc ataaattctt tcttgcttgg caaaagtccg ggcatgacga tttggaaaat
     7741 agaccacaac tcacccagat ggttttcaat cggagttcct gacaaggcaa agaccgacgg
     7801 caccacaaat tgtctcaagg tctgggcaat cttggtctgg gcatttttca tgacctgagc
     7861 ctcatctaag aaaaggaagt caaaggccat cccctgataa aactcactgt cctgacggaa
     7921 ggtggcatag ctagtcacat agatttgatg gctctcggca agaatctcct cacgacttgc
     7981 tttcaaacca tgaacaacag ccacatccaa ctgtggggca aatttctgaa actcatctgc
     8041 ccagttgtaa atcaaacctg acggagcgag aatcaaaacc cgactttctt ttgtcacttg
     8101 actagtcaaa aaagcaatgg tctgaagggt tttcccaagt cccatatcat cagccaaaat
     8161 cccaccaaaa ccataatgat ggagcatctg caaccagcca attccctttt cctgataatc
     8221 tcgcaagtca gcctgaacct gagttgcctg tcgaggaaag tcctctggat gtgtcaaatc
     8281 ctgggccaga ttctggaatt cttgtgaaaa agaaacacgg tctcgccctt caaagagatg
     8341 agctaaactg taggccaagg atttccgagc ctgcaaggtc ccatctttta attcaaattg
     8401 ccccagttcc tgtagatttt ggcgaatttt cttggtttct tcatcgaaaa agtaaacttg
     8461 attagacgaa tcaatataaa aatcctgatt ggcaaccaag gcctgcatgg cttggtcgat
     8521 ttcctcctgg gcaatatctt gaaaatcaaa ctggatttcc aagagacctc ccttggaggc
     8581 aatctgcacc tgaggactcg ctaggctata aagctcttct agtttatctg ataggtcaac
     8641 atgcccgagt ttttcaaaga ctggaatgat atcatgaaaa aaatgataga cagactccgc
     8701 ttttaaggcc tgacgccaag attgaaaatc ggcttcaaag cccgcagcca aacagacttg
     8761 gaaaattctt tcttctaagt ccgcgtcact tgaaaatggt aattcttcta gctcttgtcg
     8821 gctagatacc tgtctatttc cataatcaaa ctgaatttct aaacgaatcc gattatcttc
     8881 ttccctgtca aagtaaaaag agggcgcaaa agttttgatt tgtagacgtt ctggagctga
     8941 aacggtgccc atctggataa aaagagtcag acaggaggcc aatttgtctc gatcactgct
     9001 atcaaattgc aggtatttct ttccttgttg acccacaggt aacgctttaa tttccttgag
     9061 aagacgcatc tgctggtctg ttaaaaaata aacctgacct ttatggaaaa gtactgctcc
     9121 ctgataaaag acattgaccc taggactctc actgatttcc atttcaaaat agtccgagta
     9181 ttctgttact gtaaaggcaa atagattggc atcagcatgc atatcctgaa aaagcagggt
     9241 ttggtagcta tccacttgat ggtcaaattg aaaatggggc aaggccatca gtaaattcac
     9301 accctgctca aaaaaggtca gagggaaaaa gaggtgccga ccttggtttt ggaaaaagag
     9361 gtctgaaacc agcccttcct ccgttagtcc gtgcaagaaa gtcaaaagtt cttggctggc
     9421 atcatcaaag gcttcccaag aaagagactc ctcataaatc ttgccaatca tatacgactt
     9481 tctctgctcg acaatcctta aaaaaagtgg aatatcccga atgacatagt atttttggct
     9541 attgatttgg ccgattctca gagtccacaa gatatgattg gttcctgctt ccacctgacc
     9601 cacagctgat aactcatagg cgcattctga ttttggagat aaaattcgat ccaaaaactt
     9661 gccacccaag gtcaccttgg tttcaacagc ctctttttct tcatgacctt cttccagact
     9721 ccacaagatt tcctgaccac gctcatcatt tttcagaaaa tgctctagcg ctgccaaatg
     9781 cacacagtaa cccctctttt gaaaaaaatc acaggcacaa aaaaccaaat catcctctaa
     9841 actatagcgc agttcttctt ctgcaacgcg agcgtagagc cgattgttct tttccttgat
     9901 gatatcaacc ttaccagttt cataaagggc aacaccttcg atacgaactt tccccggaat
     9961 caatttagcc atattttcac ctttacctta tctttttatt ataccatatt ttcgcctatg
    10021 aaaatagcct tctaggaaga cttttctcct agaagactgg atttttaacg tttggcaaaa
    10081 gtagccacaa tccgctgaca gacttcctgc aacagagatt tgggcatagc tatattgatg
    10141 cgggcatgga gacttccttc ctctccaaaa tccaaaccac ggttgaggat aaccttggat
    10201 ttatttctca acaactcttg caatgtttca tcagtcaggt cataggctga aaagtcaagc
    10261 caaatcaagt aggtaccttg cggtttcatg accttgattt tagtctcttt tccaaataga
    10321 tctaccacat aattgatgtg gtcttcaaag acttgcttga gttcctctag ccaatcttta
    10381 ccgtatcgat aggcagcttc tgtcgccaaa taacccaagc ctgaaatttc atgctgatta
    10441 ttggccaaca ggcgtttctg gaaagccagt ctcaacttag gattttcaat gactgcatag
    10501 gaattttttg ttccagcaat attaaatgtt ttagtggcac tgctcaagac gatagcaaaa
    10561 tttttgaagg caggattgat ggtattgaaa gactggtgtt tgtgaccaaa gagggtcaaa
    10621 tcttggtgaa tctcatccga aactaacaaa acaccgtgtt tttggcagag ttggccaatc
    10681 ttctccaaca cttctttttc ccaaacacgt ccaccaggat tgtgagggtt gcaaagaata
    10741 tagagtttaa cctcctcttc caccaaatcc ttttcaagtt ggtcaaagtc aatctcaaac
    10801 agaccatcct tttccactaa ggaattagta atcaatctac gattattcaa cttgacactg
    10861 cgagcaaagg gtgggtagac aggcgtgtta attaaaaccg cctcgccttc ttttgtaaag
    10921 gtttgaatag ctgttgagat ggctggtacc acaccctcga taaagacaag agcctctttg
    10981 tcaaagttgt aaccgtattg tgtagcttcc cacttttgaa cttccttaat taagtcttca
    11041 ctggcatagg tataaccata aaccagttgg tctgcgtaag tttgcacggc ttggcggatt
    11101 tcaggcaaga ccacaaagtc catatccgct atccaagctg gtagaacttc actatccgtt
    11161 tctgtttctt tccatttata ggtatggtgc cctaaacggt tgggcaggct tgtaaaatca
    11221 tattttccca tctttgtctt atccttctat ggcttggcgc aaatctgcaa tcaaatctct
    11281 agcatcctca atcccaatag acaaacgcaa gaggtcatct gtcaaaccat aagaatggcg
    11341 tacctctgct ggaatatcag catgagtttg agtcgttgga taagtaataa gactttccac
    11401 tccacccaaa ctttccgcaa aagagaagag cttgagactg ttcaaaatat gaggaatgcg
    11461 tgtttcatcg gctactttaa aggaaatcat gcctccacga ccagtgtaga gaacttcctt
    11521 aactgctgga gaatccttca aaaaggcaac cacttcttgg gcgttagctg ttgagcgctc
    11581 catacgaaga gacaaggtct tgagaccacg aagcaactgg tagctgtcaa atggagacaa
    11641 gactgcccct gttgtattaa gattgtaaaa aagcttctcg tatagttcta aactattggt
    11701 cacaaccact ccagccaaga catcattgtg gcctgctaga tacttggttg ctgaatggag
    11761 aacgatatct gctccatctt caatcggacg ttggtagata gggctataga aggtattgtc
    11821 caccaccact ttggcaccct tagcatgagc caattttgct agtttttcga tatcaaattc
    11881 caacatcaag ggattggttg gggtttcgat atagagaaca tccacatcct tttctaactc
    11941 ggcaatcaac tcttcttctg tattggcata ggtaaaatgg aaacgacctt cctgctccac
    12001 ttggttaaac cagcgaaaag aaccgccgta aagatcacgc actgccaaga ccttacttcc
    12061 tactggaaag ccgctaaagg ccagtacaat agctgacatc cctgagctag tcgctaaggc
    12121 atagtctgct gactcaatag tcgccaagac ttcctcagcc ttactacgag ttggattttt
    12181 agtgcgcgta tagtcaaacc cagtagatcg accaaactct ggatgctgat aggtcgttga
    12241 aaaatgaagc ggtgtcacca atgcacctgt cgcttcatct gacttaatac ccgcctgggc
    12301 caaaattgtg ttaatgtgta attccttgct catacaatac ctccaaatct atagtaacta
    12361 ttgtaccact tattttcatc aactcatttt cttcttttca agagctagtt atagtttcaa
    12421 actatataaa aagggagttt ttcctgctcc cttaaataga ctataaaatg gtgaatctca
    12481 aaagacactt cactctatca tttgctcctg cacaaaacga gcataacgct catgattttc
    12541 cagtagttcc ttatgagttc ctgagccagt gattttcccc tcctctaaga agaaaataca
    12601 atccacatct tttaccgttg acaaactatg cgctataatc acaaccgtct tctcctttaa
    12661 tacagaatag aggctactga taatcgcata ctcagaatcc gcatcaagat tagcagtagc
    12721 ttcatcaaat ataagaattt cagcatcttt taagtaggtt ctagctattt gaagtctttg
    12781 gctttgcccc cctgacaaga atcgtccgcg ttcaccaact tcagtatcta gtccctcttt
    12841 catggagcga atctcatcac ctagtgatac taagtctagc actttcatca attcatcatc
    12901 agttactaag cgattcaaac cgagacaaag attgtcacga atactgccag ataagactgc
    12961 attattttgt gaaacccaag cgattttact tctccattct tttaagttaa aatcatatat
    13021 acttgattgc tccattagaa tatctcctga aagcggttta taaaaccgct ctaacaaacg
    13081 cacaatcgtt gattttcctg atccagatgg tccaacaaaa gcaatttttt gccccttgaa
    13141 aattgaacaa gtaatatcct ttaagacagg tcgattttca tcataaccaa aatagacatg
    13201 gttaaaattc aacccttgtc ctgataccga ttttcctccc tcaaattttt ctttaggaac
    13261 tgcaagcaag ttctccagtg caactgaaga tcccttgctc ctagaataaa cagttacaaa
    13321 attagctata ttactaatag gattaagtaa ttgaaagagg taaatcaaaa acgaaaccaa
    13381 ggttcccaca gatatatatc ctgcgctgac ccgataaccc ccataggtta gcatcacagc
    13441 tatagccgca aagataaata agagagcaaa cgaggtctca aaagaagtaa ccctatctga
    13501 tttcagtgaa ttgttttgta ccctttcaat acaattatcc aaaacatcct gtacactttt
    13561 ctctgcttgg ttagtcttaa ttaattcatg ttcttgaatc ttttcagtca attgccctgt
    13621 taaaaatccg atttcctcct gtaaacgacg actatacttt tcactgatat tggaaagggg
    13681 caagataata aacatcatac aaggaagagt gatgaataaa agtagagaaa gattccaatc
    13741 aagactaaat aagactacaa tggaaccaag taccataact aaactcagaa taatatttgg
    13801 gaaagtcgta attaaaaact cacgaatgac actcgtgtca ttgacaatgg cagaagtcaa
    13861 ctccccactt tagctcttat caaagaagga tttctctgca taaatcaacc cctctatcac
    13921 ttttttcctg atttttgcta tctttttttc acccgattga ctaaacagat agtaaccaat
    13981 agaagaaaac aaggcttgac caataaaaat caaaaacgat tgaaatactt tggaacctat
    14041 attttcaata gaactcccat ctattaaacc ttttaagata aggggaagca acaaagcaag
    14101 tagactagac agaacaagga agaaactccc cataatcacc ttagtatcta ctcttaataa
    14161 ttttaatttc ataaatactc cttataatat ttcaacggat aaagtcggga ataactcaat
    14221 ttgaggataa aatctaataa atcttcctat aacaaaacgc ataacatcta ggattttata
    14281 tacctgatat tatgcgtttt taagcacaaa gacttcttac acaaacttat ctacaattag
    14341 attttatttg acatgttttg ccaattcttc ttgggctttt ttattggatt cttctttttc
    14401 tttcaaccat ttttctctag cttttgcata ttcgtctgtt gtgacaatct tatcttgtac
    14461 tttgaggtat ttatatgatt caatcccttt tgtaccggtt aaaccatagg cagcagcaaa
    14521 tggtacggtt cttctcaatg atggtgttcc cccacgcgaa acacttggaa gaactaaaga
    14581 actatcaatc aaccaagctt gaatatcagc atatttctca taacgtttgg ctggatcttg
    14641 ctctttatta gcttcttcca acatttgagt atagacatcc agtccaactt ccttagcctt
    14701 gtcattggct tctccaggct ctagtccaag attttgtaga actcctccac tattagtatt
    14761 aaaaatatcg agataggttg acgggtcttg ataatcaggt ccccaaccgc catgatataa
    14821 atcatagtct ttctgagcag ctgtttgagc aaagtagcct gaactgtcaa actcatctga
    14881 tgttaattgc tgaatgtcaa tcactacatt atcagaacct aaaacagatt caattgattg
    14941 tttgatagaa ctaactcctt gtatgcctac tttatctgtt acttccacag tcttatccaa
    15001 gtggattggg aattgaacac cctttgcttc gagttctttc ttagcttccg caaacttagc
    15061 cttggctttc tcaggattgt agtaagggtc ttgaccatcc gcaaagttga taccttgcca
    15121 ttccttacca tagttgacca tcttagaggc tacaacttca ccaaagtctt ttcccttgat
    15181 actgacaaag tttggaggaa ccactaggtt acgcaaaatc tttgttgcac cttctttccc
    15241 ttcagactga gccccataag atgttctgtc aaaagcaaaa ttgatagcct gacggaagtt
    15301 tttattgaga actgcttcct gagtcgattt cttttcaatg tcacttgttt tagaagtata
    15361 attgtaagac ttcctatcta ggttaaaatt aaagaaatat gaagttgaat tttgcatact
    15421 atagatgata ttgtttttgt atttttcttt aatcccttca tagctggagc tgttaggaaa
    15481 aagacgagcc gtagtataag caccagctgt aaaattacgt tccagtgatt cttggtcgct
    15541 accatcatag taggtcaatt tcacatcgtc tacaaagaca ttcttagcat cccagtaatt
    15601 agggtttttc ttatattcaa tagcagattt tgagacaagt gctttcatca agaaaggtcc
    15661 attgtacaaa atactagatg gatccgcctt cccaaaatca tccccttttg atttcaggaa
    15721 atctgcatta acaggaaaaa gtatcgttgc aagtgttttt gaattccagt aaagttctgg
    15781 tttaaccaaa gtatattgaa ccgtttggtc atcaagtgcc ttgacaccga cagttgaaaa
    15841 gtcgcttgtt ttaccagtga tatagtcatc caaaccagca acagagtcct gcactagata
    15901 caaggcttct gattttttat cagctgcata ttgcaaacct gtcacaaaat cctgggcagt
    15961 tacaggcgca tattcttctc cctcagaagt aaaccacttg gcatccttac gaagtttgta
    16021 ggtataggtc aaaccgtcct gagaaacagt ccaatcctct gctaatgatg gaataatatt
    16081 cccatattgg tcattttcta ataacccgtc taccaaattt gcaacaatat cggatgttgc
    16141 tgcgcggttt tctgctagat agttcaagct agatggatca cttgaataaa catagttgta
    16201 tgtttttgac gccgtgctag aatttccaca cgcgctcaat aaaactcctg tacccaggac
    16261 aagacctgcc aaggttagat atttgctctt agactttttc atttccggaa cctcctgctt
    16321 taaatatttg ttttattata caacgtttag tttgtttagt caatgatttt ttccaaaaaa
    16381 tcgaaaaaag cctcattttc tatttttgaa atataatttt agctattttt acgctaaaaa
    16441 aggaataagc ttcaagttct cctaaaagat tgttcaactt attcctttta tttataaatg
    16501 ctaccttacg aaagcttaaa ttttgctcgc aggcgatctg cttgggcttt tcctattacc
    16561 ttatctaata aataggtacg cagactcata accatataaa gtccaccccc catggcaccg
    16621 acaagagcta cataaaagaa gctccacaaa cgtccacttg gttggaagaa aaatcctaac
    16681 agccactgga tggttcctat taacagaaac atgactaggg tcagcaaact gattaaaatg
    16741 gttcgcttca aaatcacctt gcgcttgaca ccagttactt tacaaatatc ccgatacatc
    16801 aagacgttag gaatgatgag agcaatggtt gttgaaatca aaggaccata actgtggaag
    16861 agggcgatgg taggtagttg caagactagc ttggcaatag aaccatagat aaaatagaga
    16921 acggccttgc ggttgcggaa catggcctga agcattggag acaagaccat gtacaagcct
    16981 aaaataatag actgcaaaac tgcaaagaca aataagccca gagccaaact atctggctta
    17041 ccatagaaga ccgtataaag aggttctcct accataacca ctccaaccgt tgctggtagc
    17101 aagaacataa agagtagggt gagactgtcc tgaacgagac gagaagctgc tttcaagtcc
    17161 cccttgacat agttttccgt caaaagtggc aaaccaacac tcccaatcga aacccctaca
    17221 gaaatcaaaa tcatcgtgat tttattagga ttggctgaga aataagaaaa catgacaacc
    17281 aagtcctcat tgctgtagtt ggtaaaccag ctcatactat tgataaaggt cagctgatcc
    17341 aaaatctgga agagctggat ggcagaccct gtcaggataa aaggaatggc ttccttaatg
    17401 gtatcaacca agagacgctt actgttaatc ttatctcctg tttcaaagac tcttttgagt
    17461 gaaccttctt gggcaaggaa ataaatcaag actgcaaaac tggctaccat accgacaaag
    17521 gcagcaaagg ttgattgggt aacggctgct agataatctc ctgaaccgag cttcataatg
    17581 ataaaggttg ctaggagcat ccagataaca cgaatgacct gctcagcaat ttggctcatg
    17641 gcatagggtt tgaggttatt catcccttgg aaaaatcctc ggataacact catagacggg
    17701 aaaatcaaga ctccccaagc caagctttgc atgattggga tcaagtcttt gcccacgcca
    17761 gacaagtctg ctagccaagg agcaaagaca tacaagacta aagcaaaaac caggcctagt
    17821 cctgtcataa agcctaagaa gctccgaatc agggcaaagc tatgctcttc ttctcgcatg
    17881 gtattatact tggcaacttg cttggccacc gcaactggaa tccccgctgt tgaaaccaac
    17941 aagaaccaag catagatatt gtaacccatg gtaaagagac catttgcctt agctgcataa
    18001 gcccccatcc agatgtacca agggataatg taaacagccc cgagtaggcg actgataaag
    18061 ttactagccg ttagccaagc agtcccccgt aacatctggg cctgctggtg attgttttcg
    18121 tgcgacatag attcctcatt caattttgat aactaggaaa aactccttat cttccattat
    18181 aaacttttcc ttgttacttg taaatttgcg actttcggtt tacaatagaa agtatgataa
    18241 aaattgaaac tgtattagat attttaaaga aagatggcct ttttcgcgaa attattgacc
    18301 aaggtcatta ccactacaac tacagcaaag ttgtttttga tagcatcagc tacgacagcc
    18361 gaaaagtaac agaagacact cttttttttg caaaaggcgc tgcctttaaa aaagaatacc
    18421 ttctttctgc tataacacaa ggtttagctt ggtatgtagc tgaaaaggac tacgaagtcg
    18481 atatccctgt catcattgtg aacgatataa agaaagccat gagtttgatt gccatggagt
    18541 tctatggtaa tccacaagag aaactcaaac tccttgcctt tactggtact aagggcaaga
    18601 caacagcaac ctatttcgcc tataacatct tatctcaagg gcatagacct gctatgttgt
    18661 cgaccatgaa cacaactctt gatggcgaga ctttctttaa gtcagcgttg acaacccctg
    18721 agagtattga tctctttgac atgatgaatc aggctgtgca aaatgaccgt acccacctca
    18781 tcatggaagt ctccagtcaa tcctatctgg tcaaacgtgt ctatggtcta acctttgatg
    18841 tgggagtttt cctcaatatc agcccagacc atatcggccc gattgaacac cctagctttg
    18901 aagactattt ctaccacaag cgtctcttga tggaaaatag ccgagcagtc atcattaaca
    18961 gtgacatgga ccacttctca gtcttgaaag aacaggttga agatcaagac catgatttct
    19021 atggtagcca atttgataac caaatcgaga attccaaagc ctttagcttt tcagctacgg
    19081 gtaaactcgc tggagattat gatatccaac tcattggcaa cttcaaccaa gaaaatgcag
    19141 ttgctgctgg acttgcttgt ctccgtctcg gagcaagtct tgaggacatc aaaaaaggca
    19201 tcgctgcaac ccgcgttcct ggtcgtatgg aagtcctcac tcagaaaaat ggagccaagg
    19261 tcttcatcga ctatgcccac aatggggata gtctgaaaaa actcatcaat gtggttgaaa
    19321 ctcatcaaac cggaaagatt gctctggttc tgggatcaac aggaaacaag ggagaaagtc
    19381 gtcgtaagga ctttggcctc ctcctcaatc aacaccctga gattcaagtc tttctgactg
    19441 ctgatgaccc taactatgaa gacccaatgg ccattgcaga tgaaattagt agctacatca
    19501 atcatcctgt tgaaaagatt gcggatcgcc aagaagccat caaggcggca atggctatca
    19561 caaatcacga attagatgca gttattattg cgggtaaggg agccgattgt taccaaatca
    19621 tccagggcaa gaaagaatcc tacccaggag atacagccgt cgcagaaaat tatttataag
    19681 aatagaaaaa atcaagggaa aatgaaaccc ttgatttttt cctattcttt agtaaaaacg
    19741 tttttcaaac cttcaacggc accttctaca gcaccttttg cgtcttctac aacgtctttt
    19801 accttagcaa ctgttttttc aacagctcct tcaagttctg tcttgctgtc tccagtaact
    19861 ttaccaaaac cttctttgac agcgcctgtt gcttgttcca atttgttttc aagtgacata
    19921 tgattgtctc ctttagttta ttccgatatg tgttaccggt tacatatagt ttataccgaa
    19981 tactaaggaa agtcaaacaa tgtactcaga aacaagaaat cacgctaagt agaaagttaa
    20041 tctctcctta tcaaaatcga tagccaactc atactttcca tcttcatttt gcacaatata
    20101 gcctaagaca accaaactgt ccacaaagat atcacggcat ttctgcataa gctggtcttt
    20161 tttgagaaat ttcaacaaaa aagccgtcat atacttgagt gcatactcag gattaacatc
    20221 tcctaaaatg tcatagagct cctgctgttt ttctgtcaaa ggatactgct gtttgacctt
    20281 gtaaaaataa ttggacaggg tcattttaat gcgcgcaaag tccgtctttt caactaaaat
    20341 agctgcattg atttgattgc gcaattccgt ctcaaaactc tgctccaaca aggcttgata
    20401 gaccggacta gcttctctga caaaaatctc ttgatccagt tcaagactat caagtgattc
    20461 aagcgtagga aaattgaggt aatagcgctt attttcacgt agaatcaagc ctgcctttat
    20521 atactcttcc atgagtttat caactgcaac atctggaaat tgagccttaa tttcccgtag
    20581 aatcacatcc tcatactggt ccagatagcg gatcaattct ccaaaaatgg ctgtctcgtc
    20641 aaacgagatg gattaaaaat ctgaatcatg aagattcctt tctttacatt gtaacagagg
    20701 cgatgctgcc aactgactag gattggtacc agcttggttc agaggaacag gcagacctag
    20761 ttctatgtaa tactccctcc aaaaatcact aatctcctgc tccccatctc caaatttcat
    20821 aggaatagtt tcaaaaatag gtaggatttc tgctatatac tgggagcagt agaaagcagc
    20881 tgcatctgga tagaaagaag cattgtaggg tgctccaaga tgtttgcaag ctctttcctt
    20941 caccgactgg atatccattt ctgggtaaac atagaggtcg tataaatgat tggactcaaa
    21001 gaagtctgcc ggttcttgac agacaacacc agcctgtcca ctagcatgat aaatcatccc
    21061 atccaaataa atggcaacat ggctatagtt acctgtggaa gtctggatgg cctgtcccat
    21121 gtctgaccca tctctcacaa aaatcaaatc gccattttct aacatacttc actcctagaa
    21181 aaaaggagcc gaaactcctt tcgatataag gcaaactaga ctttccgatt ttgagctgac
    21241 acccaaaatc ttacgcatta aagctttcag tcaattgagg taccacttgt ttcttacgtg
    21301 aaacggcacc agcaaggaag gcatgattgt tttcaagttt gaagttgaaa gccgcttcga
    21361 ccttgtccat attggcacca agagccaaga tttctgagtt tgagttgacg atatctgtaa
    21421 tcatcaagac aaagtcagag tagccgtttg attcgttggc agcttgcatt gcagcttcaa
    21481 tttctgcttg gcgttccaaa acttcagcga tgtcaactgt gttcacttgg gcaacacgga
    21541 cattatttcc gttgagttca aaagtcttag catcgatgtc aatcaattct tcagcagatt
    21601 tgctagccaa gttggtacca gctttcaaca ttgccaaacc atattcttcc aagttcacac
    21661 cagccaattc agccaattca ggagcaatga ttttatctgt tgggtgtgtt gttggtgatt
    21721 tcaaaagaag ggtatctgaa atcaaacctg aaagcatcaa acctgcaatc tctttaggca
    21781 cagctacacc atattctttg aacatacggt aaacgattga agacgctgat ccaactggct
    21841 ccaaacgcat gtaaagtggg cttgcagttt caaagttagc cacacggtgg tggtctacaa
    21901 caccgtaaac ttctacttca gcgatatctg atacagattg ttggaattca ttgtggtcag
    21961 tcaagataac ttgctctgcc ccctctgctt tggcagaagt gataacacgt ggtgcttcca
    22021 caccaaaata gttcaagaca aaggctgttt cttcatttgg agttccaagg gcaacagctt
    22081 ccgtatccaa accgtaagct tcttttgcaa ggtaggcaaa agctacagat gacccgatgg
    22141 catctgagtc tggattttgg tgaccaaata ctagaatctt ggacatgata atacctcatt
    22201 ttgtttattt tctttattgt agcatttttt tcgctttttg acaaaggtaa gaggagtcaa
    22261 aggactcccc acgattcttc aaaataactg agtaaatttc tatcttgatt ttcagataaa
    22321 aattcttcct tctgtggcct cttcttacgc ttgagaagag cttcagccga catggcttct
    22381 tccttactgg caaaaccttg agcatagata agtttgactg gcaagcgtgc tcgtgtatat
    22441 ttggctccct tcccactatt gtggatagcg aggcgtcttc tcacatcagt cgtatagcct
    22501 atatagtagg atccatcacg acactccaga acgtacatat aagccttatg atccataata
    22561 aatctcttcg atttcgggcg tataagagcc atcatcattg tggacaatca aaggaggtaa
    22621 gaccttaaag ccacttgttg agccatcctt gatcgcctca atcaaaagca tattggcttc
    22681 cttttctctt tttggataaa caaactgcag gcgcttaggg gctagattat gtcgttttaa
    22741 cgtatccaaa atatccagaa gtcgatcagg acgatgaacc atggccaaac gcccattaga
    22801 cttgagaata ctctgggcac tacgacagat ttcttccaaa ttagtcgtga tttcgtgtcg
    22861 agccaagaga taatgttcac tctcgttcag attagaataa ggatccacct tgaaataggg
    22921 tggattacac aaaatcatat ccaccttact cccctgaatg tgagcaggca tatttttcaa
    22981 atcatcgcag atgacctgca tttgctcctc taatccattc aaacggacag agcgttcagc
    23041 catatccgcc aaacgctcct gaatctcaac agacaatatc tgtgcttgag tacgagtgct
    23101 agcaaaaagc cccactgctc cattcccagc acagaaatcc acaatcaacc ccttcttagg
    23161 aaaacgtgga aatcgtgata agagaacact atccaccgaa tagctaaaaa cctctctatt
    23221 ttgaatgatt ttgatatctg tcgaaaagag ctggttaatg cgctctcctg attttaataa
    23281 ttgttcttct tccatggtcc tattatagca aattcatatt aacattacaa aaaatataaa
    23341 actctaaact acttcttctt ttttaaatgg tgcagggctt ctccagtcca gattggtagc
    23401 attcgtcgaa agggagcaaa accgtagtta aagcggtcgc ttgaaaagcg tctccgtcta
    23461 ggaaactggt acttttcttc ctccaaagtg cggatagaaa gactggcttt ccccgtaaat
    23521 tcatctaaat ccactacctg aacttgaacc tcttcatcga ctttcaaggt ttcatgaata
    23581 ttttcaataa atcctgtccg aatttctgaa atgtgaatca gccccgtatc acccgtctct
    23641 aactcaacaa aggcaccgta gggctgaatc cctgtaatac gcccctttag cttatcaccg
    23701 attttcatct tagtcctcga tttcaatagt ttcaattaca acatcttcaa ctggcttgtc
    23761 catagctcct gtctcaacag cagcaatggc atccaagaca gcgtaagatg cttcatcagc
    23821 taactgacca aaaatcgtgt gacggcggtc taggtgaggt gtcccacctt gattggcata
    23881 gatttctgca atcggttctg gccaaccacc acgagtaatt tctttcttag aataaggtag
    23941 gtgttggttt tgcacgataa agaactggct gccgttggta tttggaccag catttgccat
    24001 ggaaagagca ccacggatat tgtaaagctc ttctgagaat tcatcctcaa aagattcgcc
    24061 gtagattgac tcgccaccca taccagttcc agttgggtct ccaccttgga tcataaagtc
    24121 cttgataata cggtggaaaa tgacaccatc atagtagcca tcttttgaaa gagatacaaa
    24181 gttagccact gttttaggag catgttcagg gaaaagcttg atacgtaagt ctccgtgatt
    24241 ggtcttaata gtcgcaagag gaccttctac tgtttcaatg tctacttgtg gaaaatgcaa
    24301 ttctttttct accataccaa atacttctaa ggcatcaaaa atgccatctt cttctaatgt
    24361 ttttgtaata taatctgctt tttctttgat tttatcatga gaaattccca tggcaacgct
    24421 gattccagca taatcaaaga gttccaagtc gttgagacca tctccaaaaa ccatgacctt
    24481 ctctggtttc aagccaaggt gttccacaac cttttccacc cccgtcgctt tggagcctga
    24541 aatcggcaca atatcagacg aatgttgatg ccaacgaacc atgcgaagtt tgtctgagag
    24601 actgtcaggc aagtgcaagt catctccctt atcttcaaaa gtccacatct gatagatatc
    24661 ttctttttca tggaaatcgg gatctacatc taagtcggga taaattggat tgatagcttc
    24721 actcatcata tcggtgcgaa tcgacaactt ggcatcatga ctcccaacca agccatactc
    24781 aattccttct tgcttagccc aagagatata ctcctcaaca tctgactttt caatctgatg
    24841 ctgataaatg acctgacctt ttttatcttc gatataagcc ccattcaaag ttacaaaaaa
    24901 gtcaggcttg agatcacgaa tctctggaac aacaccaaaa atgccacgtc cagaggcgat
    24961 tcctgttaaa attccttttt cacgcaactg tttaaaaaca gtgggaattg tagttggaat
    25021 aaaccctgtc tttgaattcc gcaatgtatc atcaatatca aaaaagacaa tcttgatctt
    25081 ctttgccttg tatcttaatt tcgcgtccat ctcactacct ctttcaatct aactctttcc
    25141 attatatcat aaagtaggca aatcccctat tttcaaaaag tttatcattt ttattttaat
    25201 ttcttggatg agaaaagaga catatttatg aaaaagctcc atcgtgcttt taatgtgttc
    25261 tcttgttttc aaactcgtaa aaagggagcc actgagccta actcgctctc tcatttcaaa
    25321 gcttgtgaaa aaagacccgt tggggtctta attcgctttc ttgttttcaa gctcatgaaa
    25381 aagagaccca accgggtctt ttctttaatc ttcgtttacg aaaggcatca aagccattac
    25441 gcgagcgcgt ttgatagctg ttgttacttt acgttggttt ttagctgaag ttcctgttac
    25501 acgacgagga aggattttcc cacgttctga aacgaaacgg ctaagaagct cagtatcttt
    25561 gtaatcaaca tattcaattt tgtttgctgc gatgtaatca acttttttac ggcgtttgaa
    25621 tccgccacga cgttgttgag ccatgttttt tctcctttat aagtttagtt gtccattaga
    25681 atggtaaatc atcatctgaa atatccaatg ggtttgttgc tccaaatgga ttttcattac
    25741 gtgaaaagtc tggtactgaa tttgtaggtg ctgaatagtt tgcagttggt gcagagtaag
    25801 ctccacctgt gtgaccctca cgcacactac ggctttccaa catttggaaa ttctcagcca
    25861 cgacctctgt cacgtagaca cgttgtcctt gctggttatc gtaactacga gtctggatac
    25921 gacctgtcac cccgataagt gagccttttt tagcccagtt agcaagattt tcagcctgtt
    25981 ggcgccacat aacgacattg ataaaatcag cctcacgttc accattttga ctcttaaatg
    26041 tacggtttac tgcaagagta aaagtcgcaa ctgctacatt tgatggggta taacgcaact
    26101 cagcgtcacg tgtcatacgc cctacaagta caacattgtt aatcatagtt taccttctta
    26161 cgcgtcaatt ttgacgatca tgtgacgaag aatgtcagcg ttgatttttg aaagacggtc
    26221 aaactcttta agagctgcat cgtcatttgc ttcaacgtta acgatgtggt aaagtccttc
    26281 acggaaatct tggatttcgt atgcaagacg acgtttttcc caagtttttg attcaacaac
    26341 agttgcaccg ttgtcagtca aaatagagtc aaaacgtgct accaaagcgt ttttagcttc
    26401 ttcttcaatg tttggacgaa tgatataaag aatttcgtat ttagccattg atatgttcct
    26461 ccttttggtc taatgacccc aagactttgc aaggggtaag tgaggttcgc tcacaacaaa
    26521 ctattatact agaaaaaatt tttttacgca agtaaaaaca ctagaattcg aaaaaacgcc
    26581 acatgggcgt tttcctgttc ttatggtttg atacggtgca acatacgtgg gaatggaata
    26641 gcttcacgga tatgttttgt tcctgctgcg aaggttacca tacgttcgat accgatacca
    26701 aatcctccgt gtggaactgt accgtattta cgaaggtcaa ggtagaattc atattctgta
    26761 cgatccatgc caagttcatc catcttagcg acaagggcat cgtaatcttc ctcacgcata
    26821 gacccaccga taatttctcc atagccttct ggagcaagca agtctgcaca aagcacgcgc
    26881 tctggatttc caggaactgg tttcatgtag aaggccttga tggctgctgg atagttcatg
    26941 acaaatgttg gcacaccaaa gtggtttgaa atccaagttt cgtgtggtga cccaaagtca
    27001 tcaccatgct caagatgctc gtagtcagca tcttcatcat tttcatgctc ttgcaagagg
    27061 tcaatggctt gatcgtaagt gatacgtttg aatggctctg caatgtagcg tttcaagagt
    27121 tctgtatcac gttccaaggt ttccaaggct tgaggcgcgc ggtcaagaac accttgtaga
    27181 agagctttca cataagcttc ttgcaagtca agcgactcat catgtgtcaa gtatgagtac
    27241 tcagcatcca tcatccagaa ctcagtcaag tgacggcgtg tttttgattt ttcagcacgg
    27301 aaaactggac caaagtcaaa gacacgacca agagccatag cccctgcttc taggtaaagc
    27361 tgacctgatt ggctcaagta ggctggcgtt ccgaagtagt cagtttcaaa gagttctgta
    27421 gaatcttctg ccgcatttcc tgaaagaatt gggctgtcaa acttcataaa accattcttg
    27481 tcaaagaact cataagttgc atagataata gcgttacgga tttgcaacac agctacttgc
    27541 ttacgagagc gtagccacaa gtgacggtta tccatcaaaa agtctgttcc gtgttctttt
    27601 ggtgtgattg ggtagtcttg agattcaccg atcacttcga tgtctgtgat gtccaactca
    27661 tagccaaatt tagaacgttc gtcctctttg acaatacctg tcacataaac agacgtttct
    27721 tggctcaagc gtttgataac atcaaacttc tcaagtccca cttcttcacc aaatttttcg
    27781 acaaagtttg gtttaaaagc cacaccttga aagaaggctg ttccatcacg caattgtaag
    27841 aaagcgattt tcccttttcc tgatttgttg gcaacccaag cgccaatcgt cacttcctga
    27901 ccaacatagt cttttacatc aataatcgtt acacgttttg tcattatttt tccttttctt
    27961 ttttattctt tatggcaaac cacctctata ttgttcccat ccaggtcaat cataaaagca
    28021 gcataataaa tcggatgctc acttcgataa ccaggaaccc cattgtctcg cccacctgcc
    28081 tctaagccag cctcataaca agcctgaact tcttccttat tttctgctaa aaaagcaaaa
    28141 tgaacaggat cttgtgttcc ctgagtcagc caaaaatcac caccaggatg agggctgttc
    28201 ggggatagaa aactaattag agaactagtc ttaaaagcca atttatagtc caaaggagcg
    28261 agaaaactcc tataaaatcc ttctgaaatt tgtaaatcct ttaccttaat ctcaaaatga
    28321 tcaatcattc tcactaccca taaatgcttt caagcgttcg actgcttctt taagcgtgtc
    28381 taggtctgtc gcatagctga ggcggacatt ttctggtgct ccaaatccag ctcctgttac
    28441 caaggccact tcggcttctt ctaagatagc agttgtaaag tctgtcacat ccgtgtagcc
    28501 tttcatctcc atggcctttt tgacatttgg gaagagatag aaggcccctt gtggtttgac
    28561 cacttcaaat cctggcacct ctgcaaggag gggatagatg gtattaagac gttcctcaaa
    28621 ggcctgacgc atgctttcta cagtatcttg ctcacctgat agagcctcaa ctgctgcata
    28681 ttgggctact gctgacggat tcgaagttgt ttgacctgca atcttggaca tggcagcgat
    28741 aatgtctgct tctccaacgg cataaccaat ccgccaacca gtcatggcat aggttttaga
    28801 cacaccattg atgaccactg tttgcttgcg aatcgcttcc gataggctag aaatcggtgt
    28861 gaactcatga ccattataaa ccaagcggcc atagatatca tctgctagga tgagaatatc
    28921 attttctaca gcccagtttc caattgccaa gagttcctca cgggtgtaaa tcatacctgt
    28981 gggattagat ggcgaattca gcaccaaaac cttggtcttg tcagtgcgag ctgcttctaa
    29041 ctgctctacg gtcaccttaa agtgattgtc ttccttagca gaaacaaaga cgggaacgcc
    29101 ttctgccatc ttgacctgat ctccatagct aacccagtat ggggttggga tgatgacttc
    29161 atcacctgga ttgaccacag ccataaagaa ggtatagaga gaatatttgg ctcccgcagc
    29221 gactgtcact tgatttgacg ctacagaata gccgtaaaag cgctcaaagt agctattgac
    29281 caccgcctta agctctggca gacctgaggt tactgtataa aaagaagcac gcccatctcg
    29341 aatcgatgca atggcggcat cttggatatt tttgggagta gtgaaatctg gctcacccaa
    29401 ggttagagac aaaatatctc taccctcagc cttcagtgct ttggcacggg ctccagcagc
    29461 caaagtcaca ctttcttcca tttctaaaac acggttggat agtttcatag gccctcctta
    29521 ttgaccaatg ctcctgtttc aaaatctact agataaaaat cagatcctga cttaacttcc
    29581 cagattggct tgtcttgata acgaccaaag gttatcttgt caatctcgcc agctcccttt
    29641 tccttagaaa ccgtttctgc tttttcttgt gaaataccct gatttagctg ataaacgtaa
    29701 atcttatggt catctttacc aatcaggaca gcaagcgctt cttgctgttt attacgacca
    29761 agaacgctgt aataagattc caagccattg tataaatcaa cctgatcagc ctgctctaat
    29821 cctgcatact gctgagctaa tttttctcct tcacttttag ctgtttgata gggtttcatg
    29881 ctaagagaaa ccatatacag aaaggaagca ctgataacca caaacaaaat cgtcatccct
    29941 agaccatact gccacagtag attatttttt gctttgtttt gtcttttttt cactcgtcta
    30001 ttttaccatc tattaagctt tattacaagt gaatataaga atactcttcg aaaatctctt
    30061 caaaccatgt cagctttatc tgcaacctca aagctgtgct ttgagcaacc aattctattt
    30121 ctcccttcaa acaaaaccga ttttgaaagt gaaacagttc ttactttttc agtcacaaat
    30181 gactagagtt tccgaaattt tacaattttc aaataaactc tgagcaaatt tcttgcaagt
    30241 tcttttgttt tgttgtaata tattttataa caacgagaga gttctcgaaa ttttaagaaa
    30301 aaggagacac atcatgtcta aaaaagtatt atttatcgtc ggatcactac gtcaaggttc
    30361 tttcaaccac caaatggcgc tcgaagctga gaaagcactt gctggtaaag cggaagttag
    30421 ctaccttgat tattcagccc ttcctctctt cagccaagat ttggaagttc caacacatcc
    30481 agctgtagct gctgctcgtg aagcagttct cgttgcggat gctatctgga ttttctctcc
    30541 agtctacaac ttctctatcc ctggtacagt gaaaaacttg cttgactggc tatctcgtgc
    30601 ccttgacttg tctgatacac gtggcgtttc tgcccttcaa gacaagtttg tcacagtatc
    30661 atctgtagcc aatgcagggc acgatcaact tttcgctatc tacaaagacc tcttgccatt
    30721 tatccgtaca caaagcgttg gtgatttcac tgctgcacgt gttaatgact ctgcctgggc
    30781 agacggaaaa ttggttcttg aagaaacagt cctaaactca cttgaaaaac aagctcaaga
    30841 cttggtcgaa gcgattcagt aaaaaataga aaattttaga aagtctaaat caattatagc
    30901 cacctaatag ggtggctttt tatttctcaa tcaagatttt cagacaaaca tcagccatcc
    30961 aaaaaaaacc ttgagcgtta ccactcaagg tttttagcag tcttttccat cataggaaaa
    31021 tcattctaag aatattcttt cattcgagat ctatcactta tcatcttctt tcgagaaatt
    31081 agtgactgct tttggctgat taatcttctt ttcttcgtcc tacatatagg ctcaatagaa
    31141 taccgctacc taaaagaaca gagatataat cagattggct tccagtttca ggtaatttgt
    31201 ccccacggtt ttctttacct gtagttggtg ctactgggtt agcatttact ggagttgctg
    31261 gttgagccgg tgttggtact actggttgtt ctggaaccgc tggcacaggt ttaaccggta
    31321 cttctggtgt cggcacattt ggtgttggag gaactggttt gtcatttgat ggtgttactg
    31381 gtttgtcttg gcatccacat tttccatctt taccgtcttt gccgtctcga ccatcacgtc
    31441 catctctacc atctttgcca tctttaataa ctgtttttgt cacagttcca tcagaattga
    31501 tgattgtgat tgtatgacta ccatcaccat tatcttcaac agaaactttt ggtgatttac
    31561 catctttacc gtctttaatc accatttctg tcttacttcc atctggatta gtgattgtaa
    31621 caacgtgagt tccatcgtta ttatttttaa cagttacagt tggtgaaacg ctatctttgc
    31681 cgtctttacc atctttgata atagatttgt attcacgacc atcagaatct acgattgtta
    31741 atgtatgagt tccatctccg ttatcaacga tgttcgcttt tggtgattta ccatcaaaga
    31801 ttgtagttgt cgttccttta ccatcagcac ctgtcacttt aatagtgtgt gaaccatcgt
    31861 tgttatcaat aacttcaagt tttggttctc taccatcacg aactgtagtt gttgtagttg
    31921 ttccgtctga gttcacaact gtgatagtgt gagttccatc tccattatcc acaaccttag
    31981 cagttggtga tttaccatca cgaactgtgg tttcagttgt cacaccttct ggatttgtaa
    32041 taacgattgt atgacttccg tctccattat cacgaacaga tgcggttgga gtacgtccgt
    32101 caacaccgtc acgtccattc ttaacaacaa actcattctt agttccgtct gggttagtga
    32161 ttgttactgt gtggcttcca tctggatttt cagtagttgt tgctgttgca gctttaccgt
    32221 ctttaccatc tttgatgata gcgttggtaa cattgccgtc tccatctgta actttaacag
    32281 tatgagttcc atcgttgtta tccgttactt caactttagg agtcttaccg tctttaccgt
    32341 ttttaacaac agtttcttta gttgaaccat ctggatttgt caccgtaatt gtgtggcttc
    32401 catctgggtt ttctgttgta gtgatattcg cagttttacc gtctttacca tctttaacaa
    32461 ctgttgttga ttcagaacca tctgggtttt gaactgtgat agtgtgagtt ccatctccat
    32521 tatcttttgt agtaatagtt ggagctgaac catcacgtcc atctataccg ttataaatat
    32581 ctttactatt taatacttta tcgacacctt cgtcgtatac cccattgtca ttaacatcat
    32641 cataaactgt aacacggata ccgttacgcg ttggagttgc agcagcatct gtagcgcgtc
    32701 taattcttga aggtgaagta atatcttcta ctttgatttt cggtgcacgg ccatctttac
    32761 cattcttacc atcttttaca gtgaaggtta ctggatcttt acctggtgtt gtgatagtta
    32821 caacagtgct ttggccatct tgtccacgtt gagtagtgat tgtaggagtt tgtccatcct
    32881 gaccgattgc accagtttct cccttagcac catcttgaac aaagactgta gttggttgtg
    32941 aaccatcatg gttatcaaca gtgatttcta cacctttatg ccctgcttca tctttacgaa
    33001 cagctgtaac tgtaggtgat tttccatcta ctccatctct aattaccgca agtggttttt
    33061 ctggatttaa tggttgtcct gggttagctg gatcttcttg gtaaacttta gtttctttac
    33121 catccttgac agtaagcaat gatttaccat ctcgtccgtc cgcgccttta gcaccatctt
    33181 taatcatttc tgaacctaga agttcatcag ttcctggtgt atatttgccg tcattgtttt
    33241 catctgtata gaatgtcaca attgtatgag atggatttcc gttttttcct tggacacgaa
    33301 gtaaatcaac ttttggagtt ttaccgttca gaccattttc tccgtcctta acatttactg
    33361 taactggatc tttacctgga acagtgaatg taatgtcagt actatggcca tctttacctg
    33421 gagttacagt tacttctgga gtcttaccat cttctccgcg ttcacctttg tcacccttag
    33481 ggcctttgat gttgccctct ttatcccagt tgccgttatt cttaacgaag acgtcgcctg
    33541 tttctgtatt aacgtattta tcgccgtctt taccttgatg tgcttctggg ttagctttgc
    33601 cgttcaacac gtctttaccg tcacgtccat cacgacctac tgcgccatcc gcacctttag
    33661 ctccttgcgc tccagttagg ccgcgttcgc cttggtctcc tttttcgcct ttagcaccat
    33721 ctgcaccttt gtccccttta gggcctttga tgttgccttc tttatcccag tttccattgt
    33781 ttttaacata gacatcaccg gtttcggtat taatatattt atctccattt ttaccttggt
    33841 ttggttgtgg atttactttt ccgtttaata catctttacc atcacgacct gcttgtcctg
    33901 tagctccatc acggatcgta gttgtcacat ctggacttcc tggattatga accgttaatg
    33961 tatgtgtgcc gttttgcccc tcagttattg ttactgttgg ggttttccca tcagcacctt
    34021 tttgtccatc ctctacaaat gttgtttgtg gacgacctcc tactggtgta attgttacta
    34081 atacaccatt tttaccattt tgaacacttc gtgtcgctgt aattgttggt gattgtccat
    34141 tagtaccatt tcttccatca gtgatagttg ctaatggttg gtttggatta agtggttgtc
    34201 ctggtctagc cggatcctct acatagactt ttgtttcagt tccattttta acagcaatta
    34261 gagattttcc atctttacct ggagttcctg gattaccttg ttctcctttt ggtccttgtg
    34321 gtcccgctgg tcctctttgt ccatctgcgc cttttggtcc ttgaactcca tcacgacctt
    34381 gaagaccttg ttcacctttg gcaccatctg caataataac ttcttttaat ttcttatcag
    34441 aagcatctaa ttcattattt ccattttgat cataatagaa gataacagtt gttgtcttat
    34501 ctgcttcatt tcgttgagtc gcaactaatg gacttgcgcc atcgaaacca tctcttacaa
    34561 cagtacttgc aacattacct ctaccgtcgt taatagtgat agtatgagtg ccgtcatttt
    34621 tattatcttt aacggttacg gttggagtta caccgtcacg acctgcctga ccttgcgctc
    34681 cagtcaggcc gcgttcgcct tggactcctt tttcaccttt agcaccatct ttaatagcaa
    34741 ttttttgaac aagttcgtct acgccctcag tataaacacc gtttccatcg ttatcaagat
    34801 agaaagtgag gttatttgta tcaccgactt tattttgatc aacacgtggt tttactgtat
    34861 cgattagatc tttagccaca ccaatttgca taatacgtgg cttaacttct ttaataactg
    34921 aattactaat tggcaatcct aggattgttt cattacccaa ttgatgaact ttatagatga
    34981 tttgacgttg tcctttttca ccttctcgaa ctaatctagt ttgaccttct ggtaacttac
    35041 tagttggaag atagacagtt tggaagtcaa tgtcttctga aacagtctta ttattagtac
    35101 caccatttgg atttgcatcc aagaggagtg gtttttctct gaagtaatag ttagagttac
    35161 tatatggatc attactagtt gcagatcctg attccccttg acctacatta aaagtaacag
    35221 gtcttaatcg aacagacaga tcaccattag ggttggctat aggtaaacta aatctaatag
    35281 tttgattact gtttgtttca acagcagatg ttaaactgta gcttgaatga gatacaaaat
    35341 ttgcaccctt tcttatatct ttagtttgat ctcttaatct gtttgttgaa atagttgcac
    35401 gagatggttc tccaaatcca ttccctttgt ctaagagaaa gttattatta acgaactcac
    35461 ctggaccaga atatgcaatt gagaattcta ctctatttcc aactctagtt gttgttacat
    35521 caaaatgaat cgggtttcca cgacgtgaat ttccattacc acggaaagta tgagaacctc
    35581 tgctaatatc agtgaaaaca gtattatttc ctagattagt tactggttgg ttcgttggag
    35641 caggcgccgc tctaaaacca ctataccctc tatcaacatc ttccttacgg atagctggat
    35701 tttggcgagc atcagaaagg ctcaaacctg gtttcaagat ttcattaata gatgctggag
    35761 ctgaagttgt ttctactttg gcagctgtgc tagttggcaa aacagcacta tcaacagctg
    35821 aacgatcagt tgttgcagct ggattagtct cttccttcgc ttcaactata ttagctttct
    35881 tcgctacagg agtttctgat acagttcctt tgttagagtt agaacgaagt tctagaccaa
    35941 taatagatga ctctgctgct gataattcag cttcagatgc atctgctttc ccaagcaagc
    36001 tttgaccagc ttggatactt gtttgcaagt tagcagcact tgcttctgta tataacttgc
    36061 tttctaactt tttagcacgc gaaagagccg ttaacaattt tgacttgtca atagcatctt
    36121 gatgcgttac ttcctctttt ttgtcttttg cttcttcaac tttttctgct ggtttagtaa
    36181 cttctccagt tttaactgga gtcgtttcta ctggattagc tacagttgga gctgcatagg
    36241 ttttctcttt attagacgca gaaacctctg ctggtttagt agctgcttct cccctaccaa
    36301 cagaaatact attggtagtt tcttgtttgt tttcttccgc ttgtactgtt tggacactag
    36361 ctgctgcacc taataccaaa gcggtaccga gcaagacact aactgcacca aagtaatact
    36421 tacgaataga ataacgttgt tgcatgctat accagtcaaa tgatttttta tgtgaatgtt
    36481 tcattttaaa ctcctagata gtattttata gtttacaatg tacaactatt tgaggcatta
    36541 atcaatattt tctgaacaat taatatattc tgtttttgca cttgaaagcc acttatatta
    36601 ttctaatttt tccgattact aacgacaatt ttcttgctct aattttttta tactacttag
    36661 acaaaaaaag atataatgaa ttatggagga aatacccgta tgagaaacct tttatccaca
    36721 aaagttcaaa gacaattacg cttaatggaa accctaattc agaatcgtaa ttggatgaaa
    36781 ttacatgaac tagccgaaaa attaggatgc acagaacgaa tcttaaaaag tgatttaaat
    36841 gaattacgta ttgcctttcc atctattaac atccaatctt ctgttaacgg aatcatgata
    36901 gatttagaag ttaacactag tgtagaagat atttatcaat attttcttgc taattctcaa
    36961 tcttttcaat tactagagta tatgttcttc aatgaggggc tacctattta tcgaacaata
    37021 gaaaatcttt actttagtag tgccaacctc tatcgactgg gcagaaatat tacaaaggtt
    37081 ctttcatctc agtttcaaat tgaattatct tttactccat ccgagatacg tggaaatgag
    37141 atcgatattc gttacttttt tgcccaatac ttttctgagc gctattattt cctagattgg
    37201 ccttttcctg accttcctga ggaagattta acagagtttg ctgacttctt ctataaaatc
    37261 acgaattatc caatgcgatt ttcaatttat agaatgtaca aactgatgat agctatcagt
    37321 atccacaggg ttaaaaatgg tcacttcatt gacctcccca accactttta caaagaatat
    37381 tatcctcttt taaagagcat tccaaatttc caggagacac ttgcttattt ctctaaacat
    37441 tttgggcttg aaatgactcc agatacaatc gctcaaattt ttatttcttt tcttcagaat
    37501 gatattttcc tagatcctca agaattcttc aactctttag aagataacag tcaagcaaga
    37561 tactcttacc aattacttag tcaaatatta gaacgcctat caaaacaata taagattact
    37621 tttactaacc acgatgagct gatttggcat ttacacaaca ctgctttctt tgaaagacag
    37681 gaaatctttt ctaccccaat cttatttgaa caaaaagcat tgacgattaa aaaatttgaa
    37741 gtttacttcc cagactttat gggaagtgcg cgtcaagaac tgactcaata ccgtcaggca
    37801 attggacagc atgaccatcc tgaacagttg gaacacttga tgtacaccat cttaacccat
    37861 gctgaaaacc tctctactca gttgttagaa aatcgaccac ctatcaaggt tttgattatc
    37921 agtaactttg accatgctat atcccttacc tttgttgata tgctttccta ttactgtaat
    37981 aaccgcttta ccttcgatat ttgggatgaa ttaaaaacaa gtcctgagat tttaaatcag
    38041 acagattacg atatcatcgt gtctaatttc tatattccag ggattaccaa gaagtttatt
    38101 tgccgaaatc atctgtcaat catgaatttg gttaatcatc ttaacacctt atcaaatgag
    38161 attcatttgt ccaatacttt ataagttatc aaaaaagtca gcataatcgt gctgactttt
    38221 ctcttatctg atttctatac ttcctttaca aaaaccaatc cctgtggttg tgacaaatct
    38281 tctcggtaaa caaatccagc aaagttcaag cgacgtgctt cccccacagt ttgtacttga
    38341 ttttctatta aagctgttag aaaggccccg cgcgctttct tggagatagt tgagtgaatc
    38401 ttcagctgac cgcctctatc ctccatgaat ttgaaggtca ccatctttgc tctgatttcc
    38461 ttagaaaata cagtctcaaa ctctgatgac aagagagaga aaatcacttc ttccttcttc
    38521 agagcttcat catagactgc cttccaatgg ctcttcaaag tcttaccagc gacttttaat
    38581 ttcatcaaaa aatccaaacg gtgaggagcc atgggtgaca aggctggaac aacaccgtac
    38641 aaagccgagg taatgaagac atgattttca agataatctt gttccgcctc ggtcagctta
    38701 tctctcttaa tgttgcggta cataagccca tcaaaaagtt tcaaggctgg atagtgttga
    38761 gcagtttgcc ttttcaaagc ttggatattt tgaaattctt ccgccgcttt ctcagctgat
    38821 accttgtaga aactctccaa ttgactggca gaatagagag ccaaggcatc aagcacggtc
    38881 tgactttctg gttttaaagg aattgcctcg atacttggca agtctgtgtt catttctttt
    38941 gctgttggga ttaaaatttt catattgata gtgtagcata ttttttagcc acaaccaaga
    39001 aattcatttg aaaaacctcg gtttgaccga ggtttttcta tttatttggg ccatcctaac
    39061 cagacagccg cgagaacaag ggctatgcca gctaaacttg ttaagataga taagaattta
    39121 tcttttttct ccttgaactg agaaaaacct atcttcaaag cgagcagtcc gagtagcatt
    39181 gaagcaacca tcaaataaaa acccatttct ttgtcctcca ttaggcttaa tttaccttat
    39241 tataacatat atttcttaaa taaacctttt ttatactcaa tgaaaatcaa agaacaaact
    39301 aggaaactag ccgcaggctg ctcaaaacac tgttttgagg ttgcagatag agctgacgta
    39361 gtttgaagag attttcgaag agtattattg tactttaaag gtcttgagat aattgtcttt
    39421 tcctacttga ccttcgaagg ttttaccatt ttcaaggtaa ggaaggtcat cggatactga
    39481 agccttgacc ttgtatatct tgccctcaac tttaaagaag tagacagtgt ctcccttgat
    39541 aacagctgat ttgaggtctg ctactactcc cttgatgctt tctgtcgttg cattgtcaat
    39601 ttcaaggtcg tttttattgg catacttgct gagcatctct tccactgtag tagcaacgat
    39661 aacattttgg tactcgactg cgtctaccag ggcgtactct ttgaccaagc cagcattgtc
    39721 ctttaagccc atgatgtaga gaggcttgtc attgaggttg atgaggattg ggaaggttgc
    39781 tttgtaggat ttctcctgaa cagcaccttc tgctgattca cgggctgatt cttctgtcgc
    39841 agaagccaag ctatacttag tgatttctcc tgttcgcata ttttcaagga tgaaaccaag
    39901 attactctca tccgcattag ccgacgtcac acctgtgtag agatagatgt cattaccgat
    39961 agacaagtaa ttatagccct tggtagtctg ggtcacgttt ttcttggaaa tcatggcatt
    40021 caagaaaccg tccttgtact tgccgttgta gttaatttgc tcaatggttt cctctgctgg
    40081 atagatcctg tccacccatt ctggaacatc tgataagctg tattccttgg tttctccatt
    40141 tgtagcatcc aagataatga ctgaagcagg acgaggaaca gcaagtccaa attgcttttg
    40201 gtaaaccgtt gctacataga aaggattgcc ctcatcgtcc acctcaaaag atggagtttt
    40261 aaagattttg gtcgggtact tcaagcgcag gtgacgtttg acatcacggt taaaatactc
    40321 cgagtctgaa tacttgattg gtgtcttcaa gtccaccaaa tccgcatttc cagttaccat
    40381 gtccaccttg atatactcac cgattccctt ggcttgattg ttaaaccatt tgatagggtc
    40441 tgcatattct agtggtgtga cccgataagg tttcccatca attgtcaatt gggtataggt
    40501 atctgccgct acgtattgcg acaccttatc ggttagggaa cccaagtagc ggtctccaat
    40561 tttttcagca gtacttctat ctaggatagg aaccttactg gtgtcactct taggaaattc
    40621 agtaaagtct ttttccgtaa ccgtgactac attggcataa tttttagcct gaaacatgct
    40681 agaagttacc aaggaaacca aagctgccaa gagaagaatt cctccaatcg aagctaacag
    40741 aattttccct aaccgattga ttttgaaacc ctcaagattt aaggcagctt ccgccttacc
    40801 gtggcgcaca tgaaccgttt tgacaaggtt tatccccttg ccaaagccaa ataagattgc
    40861 cacaactaac aaatgcccac agaggaagaa gagaaattcc cagctggtca ggttaagggg
    40921 cggtaaaaag atataccagg tcgttgcgat aaaaataagt tcaaagacta ttcgtttcat
    40981 ttgctttcct cctaaatgat tcgtccttcc aagtgatcca gctcatgctg gcaaatctga
    41041 gctgggaagc ctgtcaaggt aatggtctgt tcctgccact tgctgtcacg ataggcaagc
    41101 cttgtggttt cataacgctt agttgatctc acacctacca aggacaaaca gccttcttct
    41161 gcctcatagg atccttcaaa ggacagaagc actgggttaa acatgaccac gggaaccaag
    41221 ccaagattaa agataatcac gcgcttctgc accccaatca tattggcagc tagaccaaca
    41281 caggtatcac gatttgctaa gagtgtatcc tgcaaatctc tggcaagata aaggtcctcc
    41341 tgacttgccg gctgagacac ttgagataaa aataagatat ccttcacaat tttcttttcc
    41401 acttcctcac actcctcttg ctttttacta tattatagca attatagcac aaaagccgat
    41461 aactgcaaac cctttccctc aactgtagca aaaagccatc cgaagatgac ttttttgcta
    41521 tttaatttct gtataagtta cttccaagcc acgcttaaca gctggacgat tggcaatttt
    41581 ttctgcccat tttactagat tttgataact tgaggcatcc aagaattttg cagaaccttg
    41641 gtaaagattt ccttgaacta actgtccata ccaagaccag atagcaatat ctgcaatcgt
    41701 atagtcattg cctgcaatat aaggtttctg agccaattcc ttatccaata aatccaactg
    41761 gcgtttcact tccattgtaa aacggttaat aggatattcc aatttttcag gagcataatt
    41821 gaagaaatgt ccaaatcccc cacctagaaa aggtgctgca cctgcttgcc agaatagcca
    41881 attcaaaact tctacctttt ccacaggatt acttggtaaa aaggctccaa atttctcagc
    41941 aaggtaaaga agaatatgag cagactcaaa gactcttaca tcttcagtac ctgactggtc
    42001 caataaggct ggaatcttgg aatttggatt gagcttcaca aagtctgatc cgaattgatc
    42061 cccatccatg atagcaatct tatacaagtc gtaagccgct tccttaaaac cagcttctag
    42121 taattcttcc aataagatag taaccttcac accatttggt gttcccagtg aataaagctg
    42181 aaaagcttgt tctccttttg gcaagttttg ttcgaaacgg gcacctgctg ttggtctgtt
    42241 tagccccgta aaagctcctt gattactagc ttcatcctgc catacggtcg gtaattgata
    42301 tgctgacatc cgaaacctcc cttaaatcgc attcttgtca aaaccgagtt tgcgttgaat
    42361 aaacttaacg atttcgacga tgataatcat tgagaagctt ccagccataa caattcccca
    42421 ttgtgacaag tctagtttgg ttacgtggaa gattccttca agcggttcta caacgattgt
    42481 tgccatgaga aggataaagg ataccaagat ggaccagtta aaggtcttag acttgaatgg
    42541 gccaactgtc aagatggatt ggtagacaga cttgacattg taggcatgga agagctgaat
    42601 caaaccaagg gttgcaaagg ccatcgttag ggcatctgca tgaatagcat gattgtcacc
    42661 cacatgaact gggtaagcaa tcgcaaggcc ataaacactc ataacaagag ctgcttggag
    42721 tacaccttga taaatgatag aactcaaaac accacctgag aagaagcttg ccttgcgtcc
    42781 acgtggttta tgattcatga cgccaggttc tgcaggttca acaccaagag cgatagctgg
    42841 gaaggtatcc gttaccaagt tgatccacaa aagatgaacc ggctgtaaga catcccaacc
    42901 aaacaaggtt gataggaaga tggttaatac ttcagcagta ttagcagaaa gtaggtactg
    42961 aatagtcttt tgaatgtttg agaagacctt acgtccttct tccactgcga cgataatagt
    43021 cgcaaagtta tcatctgcaa gaatcatatc agaagccccc ttagaaacct ctgtaccagt
    43081 gattcccata ccgataccga tatcggctgt tttcagagct ggcgcgtcat tgacaccgtc
    43141 acctgtcatg gcaacgactt taccttgttt ttgccaagcc ttgacgatac gaaccttgtg
    43201 ttctggagac acacgggcat aaacagagta ttgaccaacg actttttcaa attcttcatc
    43261 tgacagttca ttgagttcag caccagttaa aacgtgacct tctgtatcgt ttgcgtcaat
    43321 gattcccaaa cgtttggcaa tggcttccgc tgtgtcttgg tggtcacctg taatcataat
    43381 tggacggatt cccgcttcct tagccacacg aacagcctca gcggcttcag gacgttcagg
    43441 gtcaatcatc ccaatcaaac cagtaaaaat taaatcattt tcaagctctt cagaagtgag
    43501 attttctgga atactatcga taatcttata agcacctgca aggacacgca aggcttgatg
    43561 agccatttca gaattgtttg tatgaatgag atttgtaacc ttctcatcaa tcggagcaat
    43621 atccccagcc ttatcacgaa gaagacaacg ttttaagagt tggtctggcg cacccttgac
    43681 tgctacaagg aaacgaccat ctggcaatgg gtgaactgtt gacatgagct tacggtcaga
    43741 gtcaaatggc aattcagcta cacgaggata tttctctaag aaacctttga catcatagcc
    43801 cttgtccaag gcatattgga taaaggctgt ttcggttggg tcaccaatca agttaccttc
    43861 cacatcgatt ttcgtatcat tggccaagac aactgaacga agtagtggca tttcaagacc
    43921 tagttcaata tcatcagctg agtcatgtag aaccgcatcg tagaagactt tttcgactgt
    43981 catcttgttc atagtcagcg taccagtctt atcagaagcg atgatttcag ttgaaccaag
    44041 tgtttcaact gctggcaact tacgaacgat ggaatgtcgt ttggccaaaa cttgagtacc
    44101 aagagaaaga acgatggtaa cgatagcagg aagtccttct ggaatggctg caacggcaag
    44161 ggcaacagaa gtcaacaact caccaagtgg atttttccct tgaatgaaga cacccactac
    44221 aaaagtaaca agggcaatga ccaagatagc ataggtcaag accttagaaa ggttgttcaa
    44281 attttgtttg agtggtgtat cagtctcatc cgcatcttga agcataccag caatatgacc
    44341 aacttcagtg tacatacctg tattgacaac aacacccatc ccacgaccat aggttacgtt
    44401 tgagttttgg aaggccatgt tgacacggtc accaatacca gcatctgtcg caagctcgac
    44461 tgacaagtct ttttcgactg gtacagattc acctgtcaag gctgcttctt caattttaag
    44521 agagttggct tctatcaaac gtaggtccgc tggtaccacg tcacctgctt caagggcaac
    44581 gatatcgcct ggtaccaatt ctttagagtc aatctctgcc atgtgtccat cacgaagaac
    44641 gcgggcaact ggactagaca tggatttgag ggcttcaata gcttcttcag cttttccttc
    44701 ttggtaaaca ccaaaggcag cgttgatgat aaccacagct aggatgataa tggcatctgc
    44761 gatatcttcc ccaccagaag tcacgactga caagattgct gccgcaacta ggatgataat
    44821 catcaaatcc ttaaattgct cgatgaattt gaccaagatt gatcgtttct cgccttcttc
    44881 gagttcattg tgcccaaatt cggcaaggcg cttttccgcc tcacttgatg acaaaccttg
    44941 ctcggtcgca tccacagcct gcaagacctc ttcagggctc tgagtataaa acgcttggcg
    45001 tttttgttct tttgacatgt gtctcctcct tgacattgtg tgcaaaacag actctctttc
    45061 tgtcatatct tttcacgaca aacaaaaaga gacctgttaa tcataacaag tctcgctgtt
    45121 taagataggg ccggaaagca tacttttcag cataaaattc ggaatgacga cactatcaca
    45181 ggtttctgcc agctactccc ttgagtagta ccattatacc aaattttggg gagttttcaa
    45241 agagtaaaaa ctgccttatt tgaatttttc cttgaaaacc agtataatga tagaatgcta
    45301 tgtgactaga aaggaagttg aatgaagcaa tctatctcaa atctcaagtt agctgagcgt
    45361 ggagccatta tcagtatttc gacctatttg atcttgtctg cagccaaatt agcagctggt
    45421 catctccttc attcatccag tttggtggcc gatggtttta ataacgtatc ggacatcatt
    45481 ggaaatgtgg ccctcttaat cgggattcgg atggcgcgcc agcctgcaga ccgtgaccac
    45541 cgttttggtc attggaagat tgaagatttg gcaagcttga tcacttctat catcatgttc
    45601 tatgtcggtt tcgatgttct aagagatacc attcaaaaga ttctcagtcg ggaagaaacg
    45661 gtcattgatc ctcttggtgc aactctagga atcatttctg cagcgattat gtttgtggtc
    45721 tatctctaca atactcgcct cagtaagaaa tccaactcca aagcgctgaa ggcagctgct
    45781 aaggacaatc tttctgacgc tgttacctca cttggaaccg ccattgccat cctagctagt
    45841 agtttcaatt atccgattgt ggataaactg gttgctatca tcatcacttt ctttatcttg
    45901 aagactgcct atgatatctt catcgagtct tcctttagtc tttcagatgg ctttgacgac
    45961 cgcctgctcg aggactacca aaaggctatc atggaaattc ccaaaatcag caaggtcaaa
    46021 tcgcaaagag gtcgcaccta cggtagcaac atctacctgg atattacact agagatgaat
    46081 cctgacttgt ctgtttttga aagccatgaa atcgcggatc aggtcgagtc tatgctggag
    46141 aatcgttttg gcgtctttga taccgatgtc catatcgaac cagcacctat ccctgaggat
    46201 gaaattttag acaatgtcta taaaaaattg cttatgcgtg aacaattgat cgaccaagga
    46261 aaccaactag aagaactctt gactgatgat tttgtctata ttcgccaaga tggagagcag
    46321 atggataaag aggcttataa gaccaaaaaa gagttaaatt ctgctatcaa ggacattcaa
    46381 attacttcca tcagtcaaaa aaccaaactc atctgctatg agttagatgg tatcatccat
    46441 accagtatct ggcgtcgcca cgaaacctgg caaaatatct ttcatcaaga aaccaaaaaa
    46501 gaatagagaa atcctttcat gagacgggat ttttctattc gtttatactc aataaaaatc
    46561 aaagtgcaaa ttaggaagcc ggtcacaggc tgtacttgag tcggcaatgt gaagccgaca
    46621 tagtttgcac tttgattttc gaatagtctt aactatcaaa ttcactgaga tactcatagc
    46681 gttcgtattt ttcaaggagt gcttcatttt tctcatccaa ttctttttgg agagtagcca
    46741 gcttaccaaa gtcagagccg ttagcctgca tttcctcttc aatagcagcg atacgttttt
    46801 ccaaggtttc aatatcacct tcaatacttg cccactcctg cttttcttgg taggtcatgc
    46861 gtttcttgtc ttctcgaacc ttgaccactt tttccttttc ggccttttgc acttgattgg
    46921 ccatatctgt ttcaaaagct ttttcatcaa gatagtcggt gtaatgacca aagaaaggac
    46981 gaatcttgcc atcctcaaaa gcgagaatct tggtcgctac cttatccaag aaatagcggt
    47041 cgtgactgac tgttaaaacg ggacctgcaa aaccttgcaa gaaattctct aagactgtca
    47101 aagttgcaat atctaggtca ttggttggct cgtctaaaag aagaacattt ggtttttcca
    47161 aaagcagttt gaggagataa agacgttttt tctcaccccc tgacaatttc tcaatcaaag
    47221 tcccatgcgt cgaacgtggg aagaggaatt gctccagcaa ctcagcgatg gaagtcgtag
    47281 aaccaccact ggtcttgacc tcctctgcca cttcctgcag gtaattgatc acacgcttgc
    47341 tttcatccaa accctcaatt tgttgagaga aataggcgat gcgaacagtt tccccaatca
    47401 caacttgtcc tgctgtcggc tcaagacttc ctgcaatcag gttaagtagg gttgattttc
    47461 caacaccatt gtccccaaca attccaatac ggtctttagc ctgaactaag agattaaaat
    47521 tttgcaaaat gggcttattt tcataggcaa aggaaacatc ctgaaactcg atgactttct
    47581 tcccaatccg actggtttca aagttcatag tcaagtctgt ctcagcacta ctgcctgaaa
    47641 cttccttttt cagatcatgg aaacgattga tacgagcttg ttgcttggtc gcacgtgcct
    47701 gcggttgtct gcgcatccag gccagttctt gtttgtagag ttgttctttt ttatgaagta
    47761 gagctgcatc gcgctcgtcc tgttctgcct taaggcgaac atagtcctgg taattgccct
    47821 gatattctgt caagcctgct cgctccaact cgaaaatccg tgttgacaaa gcgtctaaga
    47881 aataacgatc gtgagtgata aaaaggacgg tcttcttaga atttttcaaa aagagggtca
    47941 gccactcaat aatcgcaata tccagatggt tggtcggctc atccaaaagc aaaaggtcgt
    48001 ggttgccaag taagacttgt gccaactgta cccgtcttct cagaccacct gacaattccc
    48061 caacaggagt agataagtct tgaatgccca atttgctaag aacggtcttg acctgactct
    48121 cgatttccca agcttggaga gagtccatct ctgccatgac acgttccaaa cgcgcctgct
    48181 tgtcctcact atagtcgagc ataatcaatt catactcacg aatgagctgg atttccttga
    48241 gttcactaga tagaaccgta tccaaaactg tctttctatc atcaaaatca ggatcctgag
    48301 tcaagtaacc aatctggtaa tcatttttag ctgaaaaagg actgacatcc ccatcaaatc
    48361 cagaaacacc agaaaggacg tccaaaaggg tggtcttgcc agtcccattg acaccgatta
    48421 aaccaattct gtctaagtca tggataataa aggaaatatc cctaaaaacg gtcttgtcac
    48481 caacggattt acttagtttt tcaacgataa aatcactcat tttttctccc tcaggtaagc
    48541 atggatggct tcacgattat tctccaattc tccatcgaca atggcaaact caatctctgt
    48601 taaaatctct cccaagtctg ggcctggctg atagccatat tccttgatca aaatatcgcc
    48661 attaatctga atctctttct tgtcatggat agtcaagctt tggtattttt ctgtgatggc
    48721 ttgtgggttg acttcttttc cttgagcttg acgaagattt tcagcctgta aaagcaaatc
    48781 tatgtcaaag cgataacaat ctcgcttgct caattctcca ttttcacgca gagccaaaat
    48841 aatcagcaaa tcctgaactt gcttggcaaa ctggcgtgag gtcttccaag atttcaaaaa
    48901 tgactgcgca ttttcaatct ccaaagccca tagtaaagcc gcccaggctt gttcagagga
    48961 ttcaaaagta aaatcagtct ccaaatcaaa cagtctgttg agcttgtcct ggctagatgc
    49021 catatcaggg agatagtcat aagcttgact ctcaatcatg gaagccaagc cccttctcca
    49081 aaatggagcc agcaagagtt tatcaaactc gacgaaggta cgctctacag aaattttctc
    49141 caaaagcggc gtcaaggtct tcatagcttt aaatgtttct ggctcaagtg caaaaccaag
    49201 actagcctga aaacggaaac cacgcataat ccgtaaagca tcttcgttga aacgctcact
    49261 agccactcca actgctcgca agacttgctt ttccaaatct tctaaaccat ggaacaagtc
    49321 aacgatttct cctgtctcat ccaaggcaaa ggcgttgact gtgaaatcac ggcgtttgag
    49381 gtcttcttct agcgatcgta caaaggaaac cgcactgggt ctgcgatagt ccacatagac
    49441 atcctctgtc cgaaaggttg ttacctcata ctcctcatcc ccatctaaga ccaagacggt
    49501 tccatgctcg attccgatat cggctgttcg cggaaaaatc tgcttggtct cttctggata
    49561 agaagacgtc gcaatatcca catcgtggat agggttatgg agaagggcat ctcgaacaga
    49621 gcccccaaca aaataagcct caaagcctgc ttctttaatt ttttctaata ctggtaaagc
    49681 cttctgaaat tcagaaggca tttgcgttaa tctcataata agtgttctaa tccatagaca
    49741 agctcatgac gcttgacaac ttctttaatt cccaaattga ctcctgtcat gaaggagatg
    49801 cgatcatagg agtcatgacg gagggtcaac ccttctccct gattgccaaa gatgacttcc
    49861 tgatgagcta ccaagcctgg caaacgaact gagtggatgc gcataccatc aaagtcagca
    49921 ccacgagcac cagcaatcag ctcttcctca tctgctgcgc cttgctgaat ggactctcga
    49981 acctctgcca tcaactcagc tgttttaatg gctgttccac tcggagcatc ctttttcttg
    50041 tcatgatgga gctcaataat ctccacattt gggaaatatt tggcagcctg cgtcgcaaat
    50101 tgcatgagta agacagcacc caaggcaaag ttaggggcaa tcaggccacc caagtcttgg
    50161 gcacgagaaa attcttttag ctctgcaatt tcttcactcg tgaaaccagt cgttccaact
    50221 actggagcaa agccattttc aagagcaaaa cgtgtatttt cgtaggcaac agctggagta
    50281 gtaaaatcta cccagacatc cgcttcaaaa ccagctaaat cagccttatc cttgaaaaca
    50341 ggaataccct gccattctga ctcagactca aaaggatcca aaactgctac caagtccaag
    50401 tctggatcag tcaataccat ctgacaagca gcctggccca tctttccctt aaaaccggca
    50461 ataattactc gaatactcat ctctactcct gtctaagata caaagtccgt aagaacacaa
    50521 agtgaaaata ggaattccaa tcaagaagtg tctacttctt ggaagaacta tctttttcac
    50581 acagggttcc aggcgtgttc aattatcaag atacaaagga ccttagctgc ctctgaaaaa
    50641 tagggaatgg cactgacttt ccacgaaagg caagacaggc atcttttttc aagaggcagg
    50701 tagtccgtgt tcaatttcta agatacaagg catcttaact agcctagaag cgccaactaa
    50761 atcactggaa tataacccag agcaatactt cctgctccta ggtgcgttcc aatgacacta
    50821 ccaaatgtag caagtgaaac atccgaaccc aagccaaaat caagcaagtg ctgacgcaat
    50881 tcttcagcct tttcaggagc attcccatga atgacaatga cccggtattg acctgaagcc
    50941 gttgtttcct tgataatttc aattaagcgc ttggtggcct tcttttcagt acgaactttt
    51001 tcgtaaactt caatcacacc ttgatcgtta aaataaagga ttggcttaat gctaagcaaa
    51061 ttgcccaaaa tggcagcccc atttgaaagg cgtccacctt ttaccaaatg atccaagtca
    51121 tctaccatga taaaggctga cgtacggctg atttgaatgg ctagcttatc ctgaatgctg
    51181 gcaaaatcat cgccctgatc acgccaatta aagacgcttt caaccatgat gcctagggga
    51241 gcacttgtaa tcaaagtgtc tgggaaagca atggttaagc cctcatagtc atcgaccata
    51301 tactggatat tttggtaaaa acctgaaatt ccagaagata ggaaaagccc caaggcatgt
    51361 gtatagcctt gttctttgag cgaagttaag atctcatcta acttggcaat acttggttga
    51421 ctggtcttag gcaattcaga agcctgagcc attttttggt aaaattcctc agcagacaga
    51481 ttgatgcctt cgacatattc ctcaccatca atattgacag gaatatccaa gacaaacaag
    51541 tcttctcttt gcaaggtctc ttcactgaga taagcagagg aatctgtgaa aacagctaat
    51601 ttcatattag aactccaaat taattcctgg taagtctaat gcaatttcag tcacttcgta
    51661 agtcaaacga ttgagcatgt tcaaacatgg acgagccaag gtttccacct cttcttggtt
    51721 caattcactt ggttcattga caatacggcc atcgatatgg tttacttgtg agattgttcc
    51781 actaatgaca aacttatcaa atacaatcat aaagctcaag atgacaatca aggaagtcac
    51841 ttgattttct tggtcatgtt ggagcaattg gaaattcaca tccaccttgg tttcaggagc
    51901 tccattttca ttttcccatt caaaattacg cgcatcaaaa tgatactgac taacaaattc
    51961 ttgttcacgt ttaagattca tgtctttctc catcggctac aatattataa gctattgtac
    52021 cataattttt tattttcatc tagttttcta ggatttagtc aatcccaatt tcagcacgaa
    52081 ctacatctgt gatagtatca acatagtagt ccacttcttc tgttgtaggc gcttctgcca
    52141 taacacgcaa gaggggctct gttccacttg gacgaacaag gatacggccg ttccccgcca
    52201 tttcttcttc catcttctcg atgatggcct tgatagctgg cacttccatg gccttttcct
    52261 tcatgacgtt ttccactcgg atattaacta atttttgtgg ataaatcgtt acttctgccg
    52321 ccaactctga taagctttta ccagttttct tcataatttt agtcaattga actgctgata
    52381 attgaccatc acctgtggta ttgtaatcca tcaagataac gtgaccagac tgttcaccac
    52441 caaggttgta gcctgatttt ctcatttctt caacaacgta gcggtcacca actgcagtaa
    52501 ctgccttgtt aataccttcg cgattcaagg ccttgtggaa accaaggtta gacataacag
    52561 ttgtcacaat tgtattttga gccaattgtc ctttttcaga aaggtatttt ccgatgatgt
    52621 acataatctt gtcaccatcg acgatgtcac cattctcatc aacagcaatc aagcggtcac
    52681 tgtctccatc aaaggccaaa ccaatagctg acccactttc tttgaccact tcttgaaggg
    52741 cttctggatg tgttgaacca acattaaggt tgatgttaag accgtctggt gtttccccga
    52801 taaccgtcaa ttgggcacca aggtctgcaa agatttgacg ggcactggta gaagctgctc
    52861 cattagctgt atccaaggca accttcattc catcaagagg agttccagtt gaaacaaggt
    52921 atccttcata cttacgcaag ccttctggat aatctaccaa aattcctaag ccttctgcac
    52981 ttggacgagg aagagtgtct tcctcagcat ctagcaaggc ttcaatttct gcttcttttt
    53041 catcatctag tttgaagcca tcaccgccaa agaacttgat tccgttatca agggctgggt
    53101 tgtggctagc agaaatcatg acaccggcac ttgctccttc agtttcaacc aagtaagcta
    53161 ctgctggtgt tgcaaggaca ccaagtttgt atacgtgaat ccctactgaa aggagacctg
    53221 ccaccaaggc cgattccagc atttcccctg aaatacgtgt gtcacgtcct acaaagactt
    53281 tcggcgcttc cgtttcatgt tgactaagaa catagcctcc aaaacgtcct agtttaaagg
    53341 ctaattctgg tgttagttct aggttagctt ctccacggac tccatcagtc ccaaaatatt
    53401 tacccattgt tataaaatcc ttttctattt ttaattcgtt tttgaactag ttgctttcgt
    53461 tgacgaagat gtctccgatg aactgcttgt acttgaattt gatgtgcttg aacttggtgc
    53521 tactggtttt gtagtcacct tcattattgt atcaaacgga gtgataactg ccggtaagac
    53581 aacaccattg cgatcgattg cctgcaaagg tactgaacca ctgtaattac ctgttatacg
    53641 ttcgctagtt ggcaagacag cgataatctt atcaattcta tccaatgtct cttggtcact
    53701 cgtaatcgac acttctttat ctgacaccat gacattttca atttgtaccc gactatcaat
    53761 ttgactaggg tcaatctctg gtacaatctt taccttatcc ttctgagcct tcttaccaat
    53821 cttgactgta attttttgcg gagtcgccac agcggtcagc ccattgggta aatcttcaat
    53881 gctcaaagga acttcaatcg ttccaacacc ggcatctgtt aggtcagcag taaccttgaa
    53941 tttacgtgta ctttcttgca tttcactagc tagcgatagg cgatttgcac cagtcaagac
    54001 cactgatact tctgaagcaa aaccgctaat aaaatactta tcactattat agcgtatgtc
    54061 aatagggaca tttgttactg tattagtata ggtttccgtt tttacctgcc tagcactggt
    54121 actgttttga aaattcgtcg ccgtagcata gacaaataag acacaagcaa aaaagagtga
    54181 ggatatgata tataaactat tttttttcat gtttccatcc tcctagccat cgttctttaa
    54241 aactaagacc cacttcctct tttggaagta agatttcacg taattctgtt tcaaattcat
    54301 caagtgttag gttgtgctta aaccttccat tataggttat cgaaattcct cccgtttcct
    54361 ctgatacgac aaaagtcaag gcatctgaga cttctgataa accgatagcc gcccggtgtc
    54421 tggtcccaaa ttccttggaa atacctgtgt tttttgtcaa gggcagatag gcagacgtca
    54481 cagcgatacg ttcttctttg ataatcaccg caccgtcatg taggggagtg ttgggaataa
    54541 aaatgttaat gagaagttct gcagaaatct tagcatccaa ggggattcct gtcgaaatat
    54601 actcctgcaa ggtacgtaca cgctgaatag caaccaaggc cccgatttta cgaggactca
    54661 tgtattcaac agacttaaca aaggcacgaa tcatctgttc ctcagcacta ataggggcat
    54721 tggaaaagaa atctgtcgct cttcccaaac gttccaaacc agtccgaatc tctggagaga
    54781 agataacaac cgccgcaata accccataag taataatttg attgattaac caagaaatcg
    54841 tagtcaaacc aatcatattt gcaaggattt gagctaaaat aaataccaaa actccacgta
    54901 ccaaaatcat aatcttggtt cctgcaatag cttttgtaaa atggtataaa atataagcaa
    54961 caatcaaaat atcaatcaga ttgatagcta tcgtccatgg acttgcaaac aaactggtcc
    55021 aatattgcag attggataat tgttgaaaat tcatccctga tatcctccct atcaaaacac
    55081 tttcgtccta ttataccatt ttcgggcatt tttttcccta tcctagtcca ttttacattg
    55141 aacaaaaata tgataaaata aactgactag aaaaaacaaa ggagaaacta tgtctcaact
    55201 ctatgatatt accattgtgg gtggtggtcc tgtcgggctt tttgcagcct tttatgccca
    55261 cctacgccaa gccaaggttc aaatcatcga ctctcttccc cagctaggtg gacaacctgc
    55321 tattctctac cctgaaaagg aaatcctaga cgtaccaggc ttcccaaacc tgactggaga
    55381 agagttgact aaccgcttga ttgaacagct aaatggattt gataccccta ttcatctcaa
    55441 tgaaacggtt cttgagattg acaaacaaga agaagaattt gccatcacaa cttctaaagg
    55501 aagtcacctg actaaaacag ttatcatcgc tatgggtggc ggtgccttca aaccacgtcc
    55561 gctggaactt gaaggggttg agggctatga aaatatccac taccacgttt ctaacattca
    55621 gcaatacgct ggtaagaaag tgacgattct tggtggggga gactcggctg tggattgggc
    55681 tttggctttt gaaaaaatcg caccaactac ccttgttcac cgcagagata atttccgtgc
    55741 cttggaacac agtgttcaag ccttgcaaga atcatctgta accatcaaga caccattcgc
    55801 ccctagccaa ctccttggaa atggaaaaac acttgataaa cttgaaatca caaaagtcaa
    55861 atctgatgaa actgaaacca ttgatctaga ccacctcttt gtcaactatg gtttcaaatc
    55921 ttctgtcggt aaccttaaaa actgggggct cgacctcaac cgtcacaaga ttatcgtcaa
    55981 cagcaaacag gaatccagcc aagcaggtat ctatgctatc ggtgactgct gctactatga
    56041 cggaaaaatt gatctgattg cgacaggcct cggagaagct ccaactgctg tcaacaacgc
    56101 tatcaactac attgaccctg aacaaaaagt acaaccaaaa cactctacca gtttataaaa
    56161 aagaaccacg agtcacatag gattcgtggt tttataattc atccgctatc ttattgattt
    56221 ttctgagtct gtgattgaca ccacttttgg tcagaggggt gctgaggcta tctgccaact
    56281 gttggataga gtagtctggg tgctgaatcc tcagttgcgc cacttcctgc aaatctactg
    56341 gcaaattttc taagcccatg atatctttta ttttactgat attgttaatg gtcttcatgc
    56401 tggcagaaac tgtccgagcg atattagctg tctcggcatt attagcccga ttgaggtcgt
    56461 tacgggtttc tcgcaaaatc ttaacccgct caaaatcatc acgtgcctgc atggctccga
    56521 ttactatcaa gaagtccata atgtcttctg ctcgctggag ataggtcaca gcccccttct
    56581 tgcgctcaag caccttggca tccagtaaaa actgttggag aagggaggca attccttgcg
    56641 cgtggtccag ataaacagaa ctgatttcca actggtactt gcctgactca gggtcacgaa
    56701 tgctcccatt tgccaagaaa gcgccacaga gataggcacg acctgcttcc tcgtccgata
    56761 aaatcgcctc atcaatacct gtttccaggc caaagaaaga gtctgccaag tgcaaatcac
    56821 ttaacaaatc ctgcaccttt tcatctgtaa aaacggtata gacgcgattc ttgcgaagat
    56881 tgctccgttg gtggtgacga atttcagatt tgatttcata gaaatggaga aaggactcat
    56941 agaggtgacg ggccagtttg gcattttctg tcacaactga caaagtcaag cccgaagtcg
    57001 agagaccgat gctaccagac attttgataa tggcagataa ttcatgccgg ctcagatggt
    57061 gttggcccag gatttcttct tttactgcta ctgtgaaact cattttttca cctgtataat
    57121 gcgcatcaac tcgtccacaa tcaaatctcc atcgtggaag gcaccgccat tttccagacg
    57181 aaggaagtta gatgaaatca cgcgcgaaac ttgcttacaa agacctacaa aatcgtgttc
    57241 cacttgcact aagtattcat caaaacggtt ggaattcatg tattcctgag gcactttttc
    57301 aatattcacc aagacagtgt cgataaaagg gcgaccaagg tgacgatgca agacttccac
    57361 gtggtcgcta tctgtaaagt gttccgtctc cccacgttgg gtcatgatat tgcagacata
    57421 ggcaatttct gccttagttt ccaaaagagc ccgcccaatt tccttaatca cgatattggg
    57481 caaaatagag gtaaagaggg aacctggccc taggacaatc atgtcacttt caaggatggt
    57541 ctgcactact cgacggctgg ccagaggcgt atcatcgttt agggcattgg tcacatagac
    57601 attgtcaatt atgcctcgat ggtctacaat atgactctct ccagccactt ctgtcccatc
    57661 ctgaaagact gcatgaaggg tcaaaggatg gtcactggaa ggataaattt tccctgttgt
    57721 atggaaaaat ttgctcaata actgcatggc attataggtt gaaccctgca tttctgacaa
    57781 gccagcaatg atgagatttc ccaatggatg gccagcaaag gctccggcat cctcagagaa
    57841 ccgatactga aagaccttct cataaaactt aggcatatcc gacatggcca caaggacatt
    57901 acgaagatca cctggcggtg tcaactgttg catatttttt cggagttcac ctgaagaacc
    57961 accatcatct gccaccgtca cgatagctgc gatttccaca tctttttccc gcagactttt
    58021 tagaatgacg ggaattccag tccctccacc aatcaccgtt atctttggtt ttctcatgaa
    58081 cggtttaccg tttcctttct gcggtctttg tcgcgatgcc cttcattaac agaccaattc
    58141 ttggataagt cctgcgccaa gcgtttagca aatgccacac tacggtgttg tccacccgta
    58201 catcccatgg caatggtcaa aacggactta ccttcctttt ggtaacttgg cagaatcggc
    58261 tcaatcaagg ccaataaatg ttgataaaag tcttctgact caggatggtt catgacataa
    58321 tcataaacag gttcatccac acccgtttgg tttctcagtt ctggtagata atagggattt
    58381 ggcaagaaac ggacatcaaa gaccaagtcc gcatcaatcg ggattccata cttaaatccg
    58441 aaagacatga cttcgatacg gaaagactgg gcttgttctt ggtctgaaaa ctgctctgca
    58501 agggttttgc gcagctcacg tggagtgagt tcagtcgtat ccaccacatt ttggctcata
    58561 tttttcaaag gtgccaagag ttcacgttcc aacttgattc catctaaaat acgaccgtct
    58621 gctgctagtg ggtgactccg tctggtttcc ttgtaacgag cgaccaattc cttatcagcc
    58681 gcatccaaaa agaggatttt gaaatccaaa ccatcttgat tttccaactc atccaaaaca
    58741 gcttgaatct ctgaaaagaa agaacggcta cgcatatcca ctaccaaggc caacttagga
    58801 ttgtcttcct taatttcaac cagctgcaaa aacttaggca agagagctgg cggcatatta
    58861 tcaatggtga aataacctag atcctcgaag gactgaatgg ctacagtttt ccctgcgcca
    58921 ctcatccctg tcacaatcac caagtgaaat tgtttctttg tcatcttttt ctccttatat
    58981 caaaagaagt ttggcaacac caaacttcaa ctagcttatc caatctctgc gatgacttca
    59041 atttcgactt ttacatcacg aggaagacga gctacctcca cagctgaacg agctgggaat
    59101 tcctctttga aggccgtttg gtaaacctca ttaaaaggaa caaagtcgtt catatcgctc
    59161 aagaagcaag ttgttttgac aacatggtca aagtctgttc ctgcttctgc caaaatagca
    59221 ccgatgtttt tcaagacttg ctctgtctgt tcttggatat tctctcctac aatttcccca
    59281 gtttcagggg ataggggaac ttgaccgcta gcaaacaaaa ggttgccaac gatttttcct
    59341 tgaacatagg gtccgatagc ctttggggcc ttatctgtat gaattgtttt tgccattttc
    59401 ttttcctcac aatttttcta agattgcatc ccaagcctca tccatccctg ccttgctgac
    59461 agatgaaaag aggatgaaat cgtcactcgg gtcaaagttt aatttctttt tgattgctga
    59521 ttcatgcttg ttccatttac cacgaggaat cttgtccgcc ttggtcgcca caatgatgac
    59581 tggaatctca taatacttga gaaattcgta catctgcaca tcatctgctg acgggtcatg
    59641 acgaaggtca actagactga caaccgcacg gagattttcc cgagtcgtta agtactcctc
    59701 aatcatgcac ccccactttt cacgttcctt tttagaaaca cgagcatagc cataaccagg
    59761 cacatccaca aagcgcatct tgtcatcaat gttaaaaaag ttcaggagct gggttttacc
    59821 aggttttcct gatgtacggg cgagattctt acggttcaac atagtgttga taaagctgga
    59881 tttaccaaca tttgaacgcc ctgctagggc aatctctggc agttcatcct gcggatagtg
    59941 ggacttatta gctgcactga gcaagatttc agcattgtgt gtattaagtt ccatagtcac
    60001 ctctaggctg tttctaggat cggtttatcc gttccatcga cagtttcttt agtgatgcga
    60061 accaatttca cattttcctg actcggcacc tcaaacataa catctagcat ggtttcttcg
    60121 ataatggagc gaagtccacg cgcccctgtc ttccgttcga ttgctttatt agcaatctct
    60181 tgaagggctt cgtcgtcaaa ttccaactca acatcatcat aagaaagcaa agtttggtat
    60241 tgtttcacca aggcatttct tggctctttc aagatgcgaa ccaagtcatc aacggtcaat
    60301 tgctcaagag ccgcaaaaac aggcaagcgt ccaatcaact cagggataat accaaatttt
    60361 tgaatgtctt cagcgatgat ttcttgcata tatgagctgt tttcgtcaat cgccttatta
    60421 ttttgaccaa atccgatgac tttttcaccc agacgttgtt tgacaatttc ttcaatacca
    60481 tcaaaagcac cacccacgat gaagaggata ttttttgtat ccacttgaat catctcttgt
    60541 tgtggatgtt tgcgtccacc ttgaggcggc acgctagcaa cagttccctc gataatcttg
    60601 agaagggctt gttgcacccc ttcaccagaa acatcacgtg tgatagacac attctcactc
    60661 ttcttggcaa tcttgtcaat ttcatccaca tagataatgc cacgctctgc acgttcgatg
    60721 ttaaagtcag caacctgcaa gagtttgagg aggatatttt ccacgtcctc acccacataa
    60781 ccagcctccg tcagagctgt cgcatccgca atagcaaaag gcacattcaa gctcttagcc
    60841 aaggtctggg caaggaaagt tttccctgaa ccagttgggc caatcatcaa aatgtttgac
    60901 ttctgcaaat ccacatcttc tgactcttcg cgtgtatcgt ggaaattgat gcgtttgtag
    60961 tggttataaa ccgccactgc caaggcacgc ttggcacgat cttgaccaat tacatagtgg
    61021 ttcaagatat ggaggagttc aattggtttt ggcacctcag acaagtctgc caagacttcc
    61081 tcaaccaatt cttctcgaat gatttcctga gctaactcca cgcattcatt acaaataaaa
    61141 gcattgttgc cagcaattat tttttgtact tcttcttggt ttttgccaca aaatgagcaa
    61201 taaaccatca tatcattttt tctatttgta gacatgattt ccttccattc tatactgtca
    61261 ttctatctaa aataaggtca tgtaaaaagc atgaatacta ttgaccagat tggtaaaggc
    61321 atttaaccaa aggaggatag aaagcccgta acgcttttta cgaaaagcct gtgctcctgc
    61381 cagaaagcag atgaaacaca gaaaagccgt gaatagacca aataaactcc gttccattag
    61441 acttcctttc tcttgcggta ttggatggta aaatcataag gattcttctc atctttggcg
    61501 taaaatttgc ttgaaactgt ctcaaaaaga gacaagtcaa gctcttcagg gaaataggta
    61561 tctccttcca cccgagcatg aatgtgagtg acaatcactt catcaaggta aggttcaaaa
    61621 gcctgaaaaa tttgcttccc accgataatg tagagattct tttcttgatc ctgataccag
    61681 tcaagaacag actggacgtc ctgaaaagta gcaaccccat ctatcttttc ttccggatta
    61741 cgcgtcaaaa tcagggtttc ccgttttgga agcaagcgac gccccatccc atcaaaggtc
    61801 acacgcccca tcaagatagc atgattcaga gttgtttctt taaagtgctg caattctgct
    61861 ggcaaatgcc aaggcagacg attttcctta ccaatcacac cctcttcatc ctgggcccaa
    61921 atagctacga ttttcttagt catgcttcca tccttttcac tgatagtact attttatcaa
    61981 aaaactcaaa aaaagactgg tttggaatag cttacaaaat agaaaaaatc tgtaagaaat
    62041 ttcctacaga tttatctatg tttccttatt tcttacaaac caggtgcttg tccaagttcg
    62101 gctgcaagca tccaaattgt tttatctgtt tcagttttag cgcctgcaaa gataccgttt
    62161 gttacatcgt caccttcttc atcagtgaca tccaaacctt tttggaaaag ttctgacaag
    62221 taacggtaga taacaagaac acgttccaag ctttcttcaa cattacggta ttcaccagct
    62281 tcttcttcga tttcactatt ttgaaggaac tctgtcaatg tagagaatgg gcttccaccg
    62341 agtgtaatca agcgttcgct gatttcatcc aattgaccgt caagagcttc catgtactca
    62401 tccatttttg gatgccatac aaggaaacca cgaccatgca tataccagtg cacttggtgc
    62461 aaagcaacgt gagctacata caaatcagca acagcttggt tcaagacttc ctttgttttt
    62521 gccaatgcta ctggcgctgc ttttgtcaat gatgttacgt cttttactgc ttcttttttc
    62581 aattctacca ttttctttac ctcattcatt attttttgta accacttctt aatgattact
    62641 accttagtat actactaagg aaaaccaata gcaagacaaa gaacttatat gagaaaagtt
    62701 gcaatagact gataatttaa gaattcttta gaagacaaaa aagagggttg gatgcttata
    62761 gtcaatggaa atccatcagc aaactaggaa actaaaccca gcttgaccaa agcacaagtt
    62821 ttgaggtcgc aggtagaact gaccaagctg atgtggtttg aagaaattcc ccaatactat
    62881 aatagattga aataagatga aaccaagtcg attaggaaag gtaaatgaat ttctaaaatg
    62941 ttttaccaat cacaaggtct ttgtctatat tcaatctact atataagctt catttccgag
    63001 caagatcttg aatttactcg aggatgaact atcataaaac aaagaaaaag agggtgctac
    63061 tatcacatcc ctctttcaaa tcatcgctta ataccaaacg ctgacatcta cgcgcccttg
    63121 gattcctttc atgtattcag aagaggtata ttgccaacct ttttctcctg aataataagg
    63181 gttttcccat tctaaagcat tcgtataggc cgctacccag tttacatgtt ttaaaatatc
    63241 tgggtgtttt aaacgcgtct gtaataaact acgatagcta tagacatata cattttgata
    63301 acccgcctgc ttcatcgtgt ccatgtactt gttgatgatt ttaacccaag tgtctgtatc
    63361 acttggagct ctcttgctct tatttacata ttcccagttc tcaacatcat aatagatagg
    63421 gtaagacagg ttcatattgt atttctttat aagttcaatg gtctgtttag cgtcattctc
    63481 agcatcggtc tcattttcag cataggtata gagatagaca ccataaggaa ttcccagacg
    63541 gtttaactcc ttaatgttat gcgccaattc cttgtcttct ttaccgctat aacctagacg
    63601 aacaatgaca ccatccacct cgttctcatc aataaccttt ttccaatcat tgatacgacc
    63661 attgtgctca ctaatatcaa tgattttctt agcatgttcg gttcccactt gttcttctct
    63721 attattaaag aaatagcgtt taccatctct gtgaacccag ccgacattca aatctttctt
    63781 ttctttgagc tcaccagagg ccgcaaagat atagcgagca ccatccataa tcacttcgtc
    63841 agtcgccatg gcaccacttc cagtcaaata gtagttgcct tgccactcat tccgagccat
    63901 atagccccac ttcttgaaat agtaccattt gccgcccact ttttgccact cttggttagc
    63961 ataagttcca tcggatttta gataaaaata agcagaatag gctggatcat agagccattc
    64021 attttgtatc atagctcctt gaccattcaa gaaataactt ccttgccatt ggcttttagc
    64081 catgtaaccc cacttcttga agtagtacca cttatttcca atcaattgcc attcttgatg
    64141 agcataagcc ccatctgact tgagataaaa ccaactcttg taattactgt catagatcca
    64201 ctcgttttgg gccatatatc cacctgattt caggtagtaa tttccatgcc attcattctg
    64261 cgagtaacga ccatctgaat taatataaaa ccaagcctta tagtagttgt caaaaatcca
    64321 ttcactttga gctttagaac catcatcctt tacataataa tccccatccc agtgggcagc
    64381 agcattagtc tgtacttctg cgctcgtttg cgttttatta gaagactgat tttgctctga
    64441 actacttgaa gctgtcgttg tatcctgcga agtttttgct acttcagttt catttgcagc
    64501 gacatgacta gttgccaagc ctagtaaaca gatacttgct aatccaattt ttattttcaa
    64561 tctttcctct cctataaaaa atgtaacaga catctgaatg ctgttccacc tagcttttgc
    64621 tacttactga ttattttaca aagtcaagca aagccaagaa gctttcagct tcaagtgacg
    64681 caccacctac aagggcaccg tcaacgtctg ggcaagccat gtatgaagca acattttcag
    64741 gtttaacaga accaccgtat tgaacacgaa ctttgtctgc gacttcttga ccaaagtcag
    64801 cagctacaac gtcacgaaca actttacaca ttttttgtgc atcatcttgt gaagctgatt
    64861 taccagtacc gatagcccag attggctcat aagcgataac tgaggcagca acttgttcag
    64921 cagtcaatcc agccaatgca gcagatactt gagcacctac gaattcagca gctttaccag
    64981 cttcgtaagt ttcaagtgat tcaccacaac agatgattgg aagcataccg ttcgcaaaga
    65041 ttgcttttgc ttttttgttg atatcttcat cagtttcatg gaagtagtca cggcgttctg
    65101 agtgaccgat aacaacgtag tcagtaccga tttctttcaa aacttgtggg ctagtttcac
    65161 cagtgaaagc acctgcattt tcaaagtagc agttttgagc agcaacttta aggtttgagc
    65221 cttttgcaac agcaagaaca gttgtcaaat caagagctgg agcagcgata cctgcttcaa
    65281 caagatctga tgaaggaagt tttgatgcaa ctgcttcaac gaatgcttta gcttcttctg
    65341 gatttttgtt cattttccag ttaccagcga taaatggttt acgtgacatt tcacatacct
    65401 cttttttcaa tttattttct atttattcta tcacaattat acacagcttg caaatcttac
    65461 tcggatttca agcctacttg catggattaa tccttccaga gatccatggc atttctgaaa
    65521 tctgcagata cctgtgtcaa ctgaggattg cttgcttctc tttctgctct cttggcctca
    65581 atttgagcca cactcttgat tccttcatgg cgccagtttc tcaaaatcgc ctgaatgtac
    65641 ttccagtttg gttttccatt caaaacagct tcacgaagag cctccttaat caagtcagca
    65701 ctggttccat cttcctttag tgtcttgatc aaatcctcaa tctcaaaagg cgtcaacaat
    65761 cgtcccaact cctgctggaa ggtttccacc aaatccttga gctggttttg aggtgttagc
    65821 tggtctgaac ttgaacgaac tgctccaaac aggtcatcca aacgttccaa ggccaaacta
    65881 gcatcaaaga gcaattcaat ttcgccattt aattcgatag tacgatactg gagcagtccc
    65941 ctttccgtca gattagaaat ggactggttg acatccgaaa tttccttgcc aatcctttca
    66001 gcaatctggc ttggcgacat ttcttctaag cctgtcgtat tttgcaaata gaaaaattgc
    66061 caaaccagaa aatcgtcgct agaaggaaag agttccttaa aatgcaagag cagggcactc
    66121 ggtaaaacca aggtacctga tttaaaagcg tctaaatatg tcataattcc tcttataaat
    66181 acccatatgc agatgcatca tcttctatct ttctccaatc aaaaggcgtc tcctgataga
    66241 ccgcatggtt tacccaatta ctgaaaaaga gggctgcaga tgaagaccaa caaagacaag
    66301 gtacctgatt aacatcatca tctttaaagt aattttctgg aatatgtggg tcgaaacctg
    66361 catcacgatc tcgaaaatac tcttttgcca aggtatcacg gtcatactcc aaatgaccaa
    66421 aactataaat ttctcgtaaa tcacgactgg ccaaaataga aaccccaacc tgaggtcctt
    66481 ctgataaaat ctcgagattg gtcttgttta agacctcttc cttagaaatc tccgtgtgcc
    66541 gtgaatgagg ggatacatag ctatcatcaa agcctctaaa tagaaggtga ccctctttta
    66601 gggtgtcctg aggataaata cctgatagct tactgtccat ctggtatttt tctacaccat
    66661 agcgcagata aagcccagcc tgagcccccc aacagatatg aagggtcgaa tagacatgag
    66721 tcttagacca ctcaagcatc tgtctaaatt cctcccaata gtccacttcc tcaaatggta
    66781 aatgctcaac tggagcaccc gtgatgatca tcccatcaaa atactcatcc ttgacttcag
    66841 gaaaagtttt atagaaggtc tccatgtgct ctgaacgagt tgttttagaa cggtggctct
    66901 ccatatagag aaaatcaata tccagttgta ggggtgtatt agccaagtgg cgcaacaact
    66961 gggtctctgt gaccattttc tgtggcatga gatttaaaat taaaatcttc aaaggacgga
    67021 tatcttggtg ggcagcacgt tgatcatcca tgacaaagat attctctgtc cgtaaaatct
    67081 caacagctgg caatttttta tcaattcgaa tcggcataat cctctcctaa ctgtactctt
    67141 tcaggaataa ttgcatatgt ttgaagacaa atctcaaaca cttagtcctt ttattatact
    67201 gcaagaagat atagttttca attatagttt ttctctaact agttatagtc tatttttata
    67261 tcctagtgta gagaaaacag ccctagggac tgttttcatt aataatgcat aagaactttg
    67321 tagtcgtagt caccaatttt ttcacggccg ttcaattcat ccaattcaac aaggaaggca
    67381 caacctgcca taacaccacc aagtttttca atcatctcga tagttgcctt aacagttcca
    67441 cctgtcgcca aaaggtcatc tacaataaga acacgttgac ctggcttaat ggcatccgcg
    67501 tgcatagtca aggtatcgac accgtactct ttttcatagt cagcagaaat aacttcgcgt
    67561 ggcaatttac ctggcttacg aacaggcgca aaaccaattc ccaactcaaa ggcaactgga
    67621 caacccacga taaatccacg agcttcaggt cccacgatca tgtcgacttt cttgtcagta
    67681 gcatactgaa cgatttcacg aacagcgtag ctataagcat ttccatcagc catcaaagga
    67741 ctaatatcac ggaaggtaat gccttccttt ggataatttt caattgttgc aatgtaatct
    67801 tttaaattca tctttttctt tctttcaaag ttttttactc tctattatag catatttttt
    67861 aagaaagaaa aaaggaaaag ttaacttcaa taattatcta acgttttgac gatttataac
    67921 tagccatcgc aataaagccc aatttctgtt tattcttagc aaacatttta tacatagtta
    67981 aaaactgctt tctattctcc tttttacaag catttacaca aattttcaaa gttcctagca
    68041 aaccttcgtc ataaatcata cccgataatt tcattaatgt catttcacca gtcaatgctt
    68101 tcacatcaca ataacctgat tctatcatca cctgttccca accatcttga gttaaaggac
    68161 ctacatttac atgaattgct tgtgataatt cctgtctgat agactcttta gcttccttaa
    68221 gaagcacatc atgtgtcaag agaagacctc caggttttaa tacccttaga tattccatta
    68281 cacatttttt cttagcttga tcggcttgca tagtcagcat agcttcattt ataacaatat
    68341 caaaactagc atcttgataa ggaagtttca ttgcatttgc tctttcaaaa ctgattaaat
    68401 gagcaacacc tgccgttcca gcagattttt tagccacttc taaagcttga gcatccatat
    68461 caacagcagt tatcttgcaa ccaaaacgct gtgccaactc aattgctgta gttcccctat
    68521 tacacgcaac ctctagtatt ctcttttctt ttgaaaatcc tccttctgca attaaccaat
    68581 ctgtggcacg ctttccacct ggacgtaagc gttttttccc caattttgct aaaaacttat
    68641 gacctgcttc tgacatttga acacctctgt tttttcttca ttataacaca aaatgatatt
    68701 atctgacaat tctaatctat tatttgataa actagaatca aaagcataca agtcaccata
    68761 actatagcat aaatttatta ttacctctct agttcgaatt caattgctca cgtagtttac
    68821 gcatatagct tcacttgtag atggtaccca caaataatac tcaatggaaa tcaaagagca
    68881 aactaggaaa ctagccgtaa gctgctcaaa gtacatcttt gaggttgcag ataaaactga
    68941 cgaaatcagt aatcatacgt acggcaaggc gacgttgacg tggtttgaag agattttcga
    69001 agagtattaa agagcatcac aatatagatg cataatatga cttctctagc aaaaaggaga
    69061 gaagcatttt gtttctctcc ctagattaac tgatatctct ccactacaat tgataaagag
    69121 atatcttttc ttgtagtgct ttgaatttta tttattttag tagattgttt tttcagtttc
    69181 tacatcgaag aagtgtgctt tgttcaagtc aaatccaagc tcaactgttg cacctgtttg
    69241 caagtagtca cgagcatcaa cttttgcaac aaactcgtct ttaccaactt ggcagtaaag
    69301 gtgagattct gaaccaagca gttctgatac agagatagtc gcttttacaa cacagtctgg
    69361 gaatgtttca aggaaagcag gttctgcatt cacgtcttct ggacggatac caaagatcaa
    69421 ttcttttcct tcgtagcctt tttcacgaag aactttcaat gctccttctg gcactttcaa
    69481 acggaaaccg tcagaaacaa tttcgctacc aaccaatttc acggtgatga agttcatagc
    69541 tgggcttccg atgaatcctg caacgaattt gttaactgga tttttgtaaa cttcttgagg
    69601 agtaccgatt tgttctacac gtccgatagt acctgtacca gcagggttct tagtagctga
    69661 cataataacg atacggtctg caagtgtcat cgcttctgtt tggtcgtgag ttacatagat
    69721 agttgtagct ccgatacgac ggtgaatttt agcgatttca gcacgcattg atacacgaag
    69781 tttggcatcc aagtttgaca aaggttcgtc catcaagaat actttcgcat cacggacaat
    69841 cgcacgcccc atggcaacac gttgacgttg accacctgaa aggtcagctg gtttacgttc
    69901 caagaattct ttcaatccaa gtatttcagc tgcttcttga acacgtttgt taatgtcttc
    69961 tttgctgtat ttacgcaatt tcaaaccgaa agccatgttg tcataaacag tcatgtgtgg
    70021 gtaaagagcg tagttttgga ataccatggc gatatcacgg tcttttggag ctacgtcgtt
    70081 gacaactacg ccatcgatag atgcagtacc ttctgtaatg tcttcaagac cagcaatcat
    70141 acggagtgta gttgatttac cacatcctga aggtcctacg aaaacgataa attctttatc
    70201 tttgatgttc aagttgaaat cttcaactga atagtgttcg ctgtttggat attttttgta
    70261 aatattttta agattcaatt ctaccatgag gtgaactcct tttgtctttt gataattata
    70321 ttatatatga aaacgcttta tatttctatg gcaagatgac caaaaaaata aacaagttct
    70381 gtgcaacttg cacaaaactt tagagaatat cggagaattt gaagaagata atctttatta
    70441 ttcttctatg acaaaagcag aaacaaggat cagttttccc atattttcgc tgattctcca
    70501 ctacatttga ctggattcta attttttaga gaaatacaaa agagctagct ttagctagct
    70561 cttttcctat gcggagagag ggacttgaac cctcacgacc taaagcggtc acaggatcct
    70621 tagtcctgcg cgtctgccaa ttccgccatc cccgcgtcga ttactttact agtatatcaa
    70681 cttttgggat gcttgtcaac actttttttc aaattttttc attttcacca accaggttac
    70741 tcagaaagtt catttagatt ttcatctact aacttagctc cgagtgtatt tttgaaatga
    70801 cctagggcaa attgatgatt ttcaggccag atggaagcaa cagctggttt aacaatctcg
    70861 atgtcatatc ctagattata ggcatctata gctgtatgta ggacacagat atccgtcaag
    70921 acacctgtta agataacggt agacactcta cgctctctca aacgaatatc taggtcagtc
    70981 cctgaaaaag ctgagtaatg gcgtttatcc atccaaaaga cacgactgtc tgaaccatgc
    71041 tcttgataaa agatccccaa atctccatat aaattccgtc cactcgtccc aatcagatta
    71101 tgaggaggaa ataacttact ttccggatgg aaacaatcgt tttcttcatg agcatcaata
    71161 gtaaagaaga tataatctcc tcgttcaaaa gctaatcgag ttaccttgct gatggcatcc
    71221 gaaatcgcct gagctggagc acctgctgtt agtttcccac tatcagcaac aaaatcttct
    71281 gtataatcaa tcgaaattaa agcctttgtc attagtaatc tcttttcttc acttcttcaa
    71341 aaatatctga aatcaagacc ttaagatagg ttcccttcat tccaagtgag cgactttcaa
    71401 taatccccgc agactcaagt ttacgaagag cattgacaat cacagagcga gtgattccga
    71461 tacgatctgc aatcactgac gcagtcaact tcccttcatt tccatttaat tcccctaaaa
    71521 ttgctgaaac agcacggagt tcggagtaag aaagggtatt gaccgccatg gtgacagcag
    71581 tacgacgacg aatatttttc tcatcttctt cacgttggaa gttaagaagc tgaatcccaa
    71641 caacggtact ggcaatctca acaagaacca agtcctcatc ttcgaatttt ttatcattac
    71701 gccaaataat caaagaacca aggcgaatcc ccgatacatg aatcggtgca atagtcgtca
    71761 agccatctgg aaaatcatct ctactctcaa cagggaaaat actcatatca tgctcaacag
    71821 gtaagtttgc ttctgtttcg taaatcatat tagccccttg aacgtagtca tctgggaaaa
    71881 tcttagtttg gaagaattgc tctacgcgat ctgtatttgt tttataacgc ataaaatagc
    71941 caagcagacg tcccttacta ttgataatgc aggcattgca atgaataata tccgctaact
    72001 gacgcgtaat agcgttgtaa ggaagctcat cctgcaactg ctcctctgag cgtttcaaaa
    72061 ttgatgtaat ttttctagtt ttttctacta aatgtgccat ttttcacctc gaatttaatc
    72121 gctatcatta taacataaaa acgtctcttt ttcaataatt atctgaaaat tccttattga
    72181 cttgcattga cttacaattt aattaaaaac cagaatattt ttaattaaat tgttcctttt
    72241 ctattgacaa gttgcctatt tttgtgtatc ataacattat aaaagataat ataataattt
    72301 tatttgtctt ttcacattcg gtctccttat ataaaaaagc gattcatttt gaaccgcttt
    72361 ttcttattta tcgcccttgt tacgaataac aaagcctgtt tgcttttcgc ttaaagtatt
    72421 gcgtggtttt ttattatcct tacggtaacg tttttcctta tcaaaacgat cgttgccacg
    72481 acttcctttt ttgaactcat cacggcgatc acgctctcga cggtcgtccc cacgacggcc
    72541 tccacgacct cccttagctt taccaccgaa accattacct gatggtttaa acggtagtgg
    72601 tttttcacgt gcaatctcca cttctggaag gctatctggg tcttggactg tcagactcaa
    72661 gatatacatt gccaattctt ctggagtaaa ctcagcagcc aatttgcgag catccttacc
    72721 aaatttctca aagttggcac gaatggtttc atctgcaaaa tcacgttcga ttttcttgag
    72781 agctacctgt ttttttgatt ggaaggattc ttctacactt gcaggtttga gacctttcat
    72841 gcgtttctta gtcaagtttt caatgatttg aaggtaaccc atttcgtttg gagcaacaaa
    72901 agtaatagat tgacctgact taccagcacg acctgtacga ccgatacggt gaacataact
    72961 ctcaggatct tgtggaatat cgtagttgta gacatgggtc acacctgaaa tatccaaacc
    73021 acgcgctgca acgtctgtcg caaccaaaac atcaagattg ccatttttaa agtcacgaag
    73081 gacacgaaga cgtttgtttt ggtctaggtc gccatgaatt ccttctgcac ggaagccacg
    73141 aattttcaaa ccacgagtca attcatccac acggcgtttg gtacgaccaa atacaatagc
    73201 gagttctggt tgtgccacat ccatgagacg agtcatggtg tcaaattttt cttgttcctt
    73261 aacacggata tagtactggt caaccaattc tgttgtcaat tccttagccg caatcttgac
    73321 atgttcaggg gctttcataa actgaacacc gatacgtttg atggcatctg gcatagttgc
    73381 tgagaaaagc aaagtttgac ggttctcagg tacacgggaa ataatggctt cgatgtcttc
    73441 aaggaagccc atgttaagca tttcatccgc ttcgtcaagg ataagggttt caatgtcttg
    73501 taatttcaag gccttgcgtt taatcaagtc caagaggcga cctggagttc ccaccacgat
    73561 atgggcacca gatttaagag ccttaatttg tttttcaatg cttgatccgc catatactga
    73621 acggactttg actcccttac tacgaccaaa gcggaagagt tcttcttgac tttggacagc
    73681 tagttcacga gttggagcga tgaccaaggc ttggatagtc gcttcttctg tacggatttt
    73741 ttcaagggta ggcaagccaa aggctgcagt ttttcctgta ccagtctgag cttgaccgat
    73801 aacatccttg ccttcaaggg ccaaaggaat agtttgttct tggataggac tagcttctac
    73861 aaaaccagct ttttcaattt ctgctagcaa atcagcagac aagtttaatt cattaaattt
    73921 cacgttattc ttctttctaa aggtggtgcg aagccaccct atagggctta gtttatactt
    73981 ttctttttat gacgtatttt catataacta gatataaaat cgtgttgctt cttttccaca
    74041 aaagaaaagt actgttttct ttgcaaccta tctagtataa cacaagacca gagcaaaaga
    74101 tagccccatt tctacagaaa atcatgtaag cggtttttga ctttcttttt tgattgaacg
    74161 acctagataa taagacaaag ccaaggcgat actgtataaa atgagaaaaa cgaacaaggt
    74221 ttgtgtgtac gaatgagcca ttttataagt ctctgctaat aaaataggtc ccgctaaacc
    74281 agccattgcc caagctgtta aaatataacc atgcagagcg gccaattcct tggttccaaa
    74341 aatatcactg agataagctg gaatcaaaga aaaaccagct ccatagcaag tcatcaaaat
    74401 agacatagca actacaaata aaacggaatc tgtaaagagc caaagtgaga gagaaaagaa
    74461 aagattgaca agcagtaata tactaaaggt tagagggcga ccgatatagt cagacaaact
    74521 cgcccagagc aagcgaccaa atccattgaa aatccccaaa acacccacca ttactgctgc
    74581 atgacttgta gacaagccag ccatctcctg tgccattggc gatgccgctg aaattaagcc
    74641 taaaccacaa gctatgttga taaagaaaat aatccaaagc atataaaacc gattgctttt
    74701 tagagcctga tttgcagcca ttccttgcgt caaagaggct gttttttctt tccctgaaga
    74761 agataaaatt gcaagctctt gctcatttgg acgcttaatg aattgtgaag ctaggagcat
    74821 gataataaag taacttgctc ctaaaatata aaaagtttct acaatcccta cccctgcgat
    74881 gaggtgttgc gctatgggac tagtcaataa agaagcaaaa ccaaacccca taatcgctaa
    74941 acctgttgcg agaccacgtt tatcaggaaa ccattttata atcgtcgaca caggggtaat
    75001 atagcctgct cccaaaccaa gcccacctaa aatgccataa gcgagataca acaaccacag
    75061 ctcctgacgg tctattgcaa atcctgctaa gatatttcca cctgcgtata gaaaagcaga
    75121 tagacttccc atgactttcg gaccaaattt ttctaccaaa cgccccataa atgcagccga
    75181 taagcccaaa caaaagattg ctagactaaa ggcgaaggca acagaagcct gatcccatcc
    75241 cgttttttca ataatagggt tacgataaac actccaggca taagtcgaac ccagcattaa
    75301 gtgtaaaatg accccagcaa aggcaataat ataacgattc gatttcataa aacctcctat
    75361 tttcgaacat ttattctatc ttatataaaa atagatagga ttttagttta tcaagcttgt
    75421 taaaaaatta ctagtttttt gtctggaaag cgtttttatc tttttctgtc tttttcgtag
    75481 cagaattgtg caaaagtttt tttcttgtgc tagaatgaaa tgcgaatagg aggaattcta
    75541 aatgttaact tatgatttaa tcgttatcgg atttggtaaa gctggtaaaa cactagctgg
    75601 taaattggct tcagctggca aaaaagttgc cctcgttgaa cgtagcaaag ctatgtacgg
    75661 tggaacttgt atcaacatcg gttgtatccc aactaaaact ttgctggttg ctgctgagaa
    75721 agacttgtct tttgaagaag tcattgctac caaaaacacg atcactggtc gcctcaacgg
    75781 taaaaactat acgactgttg ctggtactgg cgtcgatatc tttgatgctg aagctcactt
    75841 cctttcaaat aaagtcatcg aaatccaagc tggtgatgaa aaacaagaat tgactgctga
    75901 aactatcgtc atcaacactg gtgctgtttc aaacgtcttg ccaatccctg gacttgctac
    75961 aagcaaaaac gtctttgact caacaggtat ccaaagcttg gataaattgc ctgaaaaact
    76021 tggagtcctt ggtggcggaa atatcggtct tgaatttgct ggcctttaca ataaactagg
    76081 aagcaaggtt acagtcctag atgccttgga tacattccta cctcgtgcag aaccttccat
    76141 cgcagctctt gctaaacaat acatggaaga agacggtatt gaattgcttc aaaatatcca
    76201 tactactgaa attaaaaacg acggtgacca agtgcttgtc gtaactgaag acgaaactta
    76261 ccgtttcgac gcccttctct acgcaactgg acgcaaacca aatgtagaac cacttcaact
    76321 tgaaaataca gatattgaac taactgaacg tggcgctatt aaagtagata aacactgtca
    76381 aacaaacgtt cctggtgtct ttgcagttgg agatgtcaac ggtggtcttc aatttactta
    76441 catttcactt gatgacttcc gtgttgttta cagctacctt gctggagatg gcagctacac
    76501 acttgaggac cgtctcaatg tgccaaatac tatgttcatc acacctgcac tttcacaagt
    76561 tggtttgact gaaagccaag cagctgattt gaaacttcca tacgcagtga aagaaatccc
    76621 tgttgcagcc atgcctcgtg gtcacgtaaa tggagacctt cgcggagctt tcaaagctgt
    76681 tgttaatact gaaacaaaag aaattcttgg tgcaagcatc ttctcagaag gttctcaaga
    76741 aatcatcaac atcattactg ttgctatgga caacaagatt ccttacactt acttcacaaa
    76801 acaaatcttc actcacccaa ccttggctga gaacttgaat gacttgtttg cgatttaagt
    76861 tgaaatctca tcttaactga cagccctctt tgggctgttt ttacttctac gaaacaccaa
    76921 atctgtcttt tccctctttt gtgatataat agaaacatga acttaaaaac tactttgggc
    76981 cttcttgctg ggcgttcttc ccacttcgtt ttaagccgtc ttggacgtgg aagtacgctc
    77041 ccaggtaaag tcgcccttca atttgataaa gatattttac aaaacctagc taagaactac
    77101 gagattgtcg ttgtcactgg aacaaatgga aaaaccctga caactgccct cactgtcggc
    77161 attttaaaag aggtttatgg tcaagttcta accaacccaa gcggtgccaa catgattaca
    77221 gggattgcaa caaccttcct aacagccaaa tcttctaaaa ctggaaaaaa tattgccgtc
    77281 ctcgaaattg acgaagccag tctatctcgt atctgtgact atatccagcc tagtcttttt
    77341 gtcattacta atatcttccg tgaccagatg gaccgtttcg gtgaaatcta tactacctat
    77401 aacatgatat tggatgccat tcggaaagtt ccaactgcta ctgttctcct taacggagac
    77461 agtccacttt tctacaagcc aactattcca aaccctatag agtattttgg ttttgacttg
    77521 gaaaaaggac cagcccaact ggctcactac aataccgaag ggattctctg tcctgactgc
    77581 caaggcatcc tcaaatatga gcataatacc tatgcaaact tgggtgccta tatctgtgaa
    77641 ggttgtggat gtaaacgtcc tgatctcgac tatcgtttga caaaattggt tgagttgacc
    77701 aacaatcgct ctcgctttgt catagacggc caagaatacg gtatccaaat cggcgggctc
    77761 tataatatct ataacgccct agctgctgtg gccatcgccc gtttcctagg tgccgattcg
    77821 caactcatca aacagggatt tgacaagagc cgtgctgtct ttggacgcca agaaaccttt
    77881 catatcggtg acaaggaatg tacccttgtc ttgattaaaa atccagtcgg tgcaacccaa
    77941 gctatcgaaa tgatcaaact agcaccttat ccatttagcc tatctgtcct ccttaatgcc
    78001 aactatgcag atggaattga cactagctgg atctgggatg cagactttga gcaaatcact
    78061 gacatggaca ttcctgaaat caacgctggc ggtgttcgtc attctgaaat cgctcgtcgc
    78121 ctccgagtga ctggctatcc agctgagaaa atcactgaaa cgagtaatct ggagcaagtt
    78181 ctcaagacca ttgagaatca agactgcaag catgcctata ttctggcaac ttatactgcc
    78241 atgctggaat ttcgtgaact gctggctagt cgtcagattg ttagaaagga gatgaactaa
    78301 tggtttatac ttcactttcc tcaaaagatg gcaattaccc ctatcagctc aacattgccc
    78361 acctctacgg aaatctcatg aatacctacg gggacaatgg aaacatcctc atgctcaagt
    78421 atgtggctga aaaactggga gcccatgtga ccgttgacat cgtttctctc catgatgact
    78481 ttgatgaaaa tcactacgac atcgcctttt tcggtggtgg tcaagacttt gaacaaagta
    78541 tcattgcaga cgacctacct gctaaaaaag agagcattga caactacatc caaaacgacg
    78601 gtgtagttct ggctatctgc ggtggtttcc aactattggg tcaatattat gttgaagctt
    78661 caggaaaacg tatcgaaggg ctaggggtca tgggacacta cacgctcaac cagaccaata
    78721 accgttttat cggtgacatc aagattcaca atgaagattt cgatgaaacc tactatggat
    78781 ttgaaaatca ccaaggccgt accttcctct ctgatgacca aaaaccgctg ggacaggttg
    78841 tctatggaaa tggaaacaac gaagaaaagg tcggtgaagg ggttcattat aagaatgtct
    78901 ttggttccta cttccacggg cctatcctct ctcgtaatgc caatctggct tatcgcctag
    78961 ttactactgc cctcaagaag aaatatggtc aggatatcca actccctgcc tatgaggata
    79021 tcctcagcca agaaatcgct gaagagtaca gtgacgtcaa aagcaaggct gacttttctt
    79081 aaacaaagga aaatgatatc aaagaactcc gttatcttgt cggagttttt tgtcttttct
    79141 tttacccttc tcccttgcat tttctctcat tttttgccaa aatagagggg tagaaagaag
    79201 gtagcatatg tctaaattac aacaaatcct aacatatctt gaatcagaaa aactagacgt
    79261 cgctgtcgta tctgaccccg tcacaatcaa ttacctcact ggtttttaca gtgatcccca
    79321 tgaacgccaa atgttcctct ttgtcctagc ggatcaggaa cctctcctct ttgtcccagc
    79381 tcttgaagta gaacgtgcaa gtagcaccgt ttccttccca gtagtgggct atgtcgattc
    79441 tgaaaatcca tggcaaaaaa tcaaacatgc tcttccacaa cttgacttca aacgtgtcgc
    79501 tgttgagttt gacaatctca tcttgaccaa ataccatggt ttgaaaacaa tttttgaaac
    79561 tgctgagttt gaaaacctca ctcctcgtat ccaacgcatg cgcctcatca aatcagctga
    79621 tgaagtacaa aaaatgatgg ttgcaggtct ttatgctgac aaggctgttc atgttggttt
    79681 tgacaatatt tctcttgata agactgagac agatatcatc gcacaaattg actttgccat
    79741 gaaacgtgaa ggttacgaaa tgagctttga taccatggtc ttgactggtg ataatgctgc
    79801 gaatccacac ggcattccag cagctaataa ggttgaaaat gatgctcttc tcctctttga
    79861 cctgggggtt ctggtcaatg gctatgcgtc agatatgact cgtacagtcg ctgtcggcaa
    79921 accagaccaa ttcaagaaag atatttacaa cttgactctt gaagcccaac aagctgctct
    79981 tgactttatc aagccaggtg tgactgctca tgaagtggac cgcgctgccc gtgagatcat
    80041 cgaaaaagct ggttatggtg agtacttcaa ccaccgtctc gggcatggta tcggtatgga
    80101 tgtccatgaa ttcccatcta tcatggaagg aaacgacatg gtcatcgaag aaggcatgtg
    80161 cttctctgtt gaaccaggta tctatatccc tggtaaagtc ggtgttcgta ttgaagactg
    80221 cggtgttgtt accaaggatg gcttcgacct ctttacaagc accagcaaag atttgcttta
    80281 ttttgattaa actatatagc ccctatgctt tcctttcaaa atatctaggg gctattttat
    80341 tgtcattttt ctgctattat gctaaagaaa ttggctgcaa taatctaacc ctaagtgtct
    80401 ggaatgataa cgagggtgct ctccgctttt atcaaagaca agggatgaaa ccccaagaaa
    80461 caacaatgga aatgataatt gattaagaag tcatctatca aaagatgtta gaaaaagttc
    80521 aatttcacta gaaaatgagg aaaatcttcc cacaataaaa cgcataatag tgggtgtaga
    80581 atgaactgcc ccccaaaaag ttagacaatt aatttatccg aaggatttag ttctgtattg
    80641 cacaggg
//
LOCUS       DAVLVZ010000002        59802 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000002 DAVLVZ010000000
VERSION     DAVLVZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59802
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.002"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(124..1104)
                     /locus_tag="VYN77_000077"
     CDS             complement(124..1104)
                     /locus_tag="VYN77_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1557622.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAVYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1420..3435)
                     /gene="recG"
                     /locus_tag="VYN77_000078"
     CDS             complement(1420..3435)
                     /gene="recG"
                     /locus_tag="VYN77_000078"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1557623.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRIQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTAHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3454..4557)
                     /locus_tag="VYN77_000079"
     CDS             complement(3454..4557)
                     /locus_tag="VYN77_000079"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1557624.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVNGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHIKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4547..4915)
                     /locus_tag="VYN77_000080"
     CDS             complement(4547..4915)
                     /locus_tag="VYN77_000080"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1557625.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(4954..5985)
                     /locus_tag="VYN77_000081"
     CDS             complement(4954..5985)
                     /locus_tag="VYN77_000081"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1557626.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(5987..7018)
                     /locus_tag="VYN77_000082"
     CDS             complement(5987..7018)
                     /locus_tag="VYN77_000082"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1557627.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7099..9612)
                     /gene="secA"
                     /locus_tag="VYN77_000083"
     CDS             complement(7099..9612)
                     /gene="secA"
                     /locus_tag="VYN77_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1557628.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9758..10045)
                     /locus_tag="VYN77_000084"
     CDS             complement(9758..10045)
                     /locus_tag="VYN77_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557629.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10073..10705)
                     /locus_tag="VYN77_000085"
     CDS             complement(10073..10705)
                     /locus_tag="VYN77_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1557630.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(10709..11350)
                     /locus_tag="VYN77_000086"
     CDS             complement(10709..11350)
                     /locus_tag="VYN77_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557631.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLVFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11343..12134)
                     /locus_tag="VYN77_000087"
     CDS             complement(11343..12134)
                     /locus_tag="VYN77_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557632.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYPGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12156..12548)
                     /locus_tag="VYN77_000088"
     CDS             complement(12156..12548)
                     /locus_tag="VYN77_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557633.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            12875..13726
                     /locus_tag="VYN77_000089"
     CDS             12875..13726
                     /locus_tag="VYN77_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1557634.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            complement(13948..14298)
                     /locus_tag="VYN77_000090"
     CDS             complement(13948..14298)
                     /locus_tag="VYN77_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557635.1"
                     /translation="MEHLFKMIILLPCFYFFSWIERANRDSKIFSIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(14438..14773)
                     /locus_tag="VYN77_000091"
     CDS             complement(14438..14773)
                     /locus_tag="VYN77_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557636.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(14862..16172)
                     /gene="der"
                     /locus_tag="VYN77_000092"
     CDS             complement(14862..16172)
                     /gene="der"
                     /locus_tag="VYN77_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1557637.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(16186..16899)
                     /locus_tag="VYN77_000093"
     CDS             complement(16186..16899)
                     /locus_tag="VYN77_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1557638.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMAL
                     GVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(16896..17792)
                     /gene="dnaI"
                     /locus_tag="VYN77_000094"
     CDS             complement(16896..17792)
                     /gene="dnaI"
                     /locus_tag="VYN77_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1557639.1"
                     /translation="MESVGDVLKRQPSRFHYQDLVQKIMKDPDVAAFVQQESLNQDEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVMNHGYADVSYEETPELIAAEKEAA
                     IKKRLNLINFPSSLKNVSFLDVYRDDVQRLTVLKRMIEFVNDYPNNLKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(17793..18962)
                     /locus_tag="VYN77_000095"
     CDS             complement(17793..18962)
                     /locus_tag="VYN77_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1557640.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDELRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKAKTQKQNQTAPAKTNIPKWSNPEYKNTSSAEELEEMERQTLELLAKLDNGGD"
     gene            complement(18963..19436)
                     /gene="nrdR"
                     /locus_tag="VYN77_000096"
     CDS             complement(18963..19436)
                     /gene="nrdR"
                     /locus_tag="VYN77_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1557641.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            19576..19941
                     /locus_tag="VYN77_000097"
     CDS             19576..19941
                     /locus_tag="VYN77_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1557642.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            19946..20641
                     /locus_tag="VYN77_000098"
     CDS             19946..20641
                     /locus_tag="VYN77_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1557643.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            20658..21425
                     /locus_tag="VYN77_000099"
     CDS             20658..21425
                     /locus_tag="VYN77_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557644.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELTFIAIFYLGTYYILRNKVNLL"
     gene            complement(21778..22977)
                     /locus_tag="VYN77_000100"
     CDS             complement(21778..22977)
                     /locus_tag="VYN77_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1557645.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(22974..23867)
                     /locus_tag="VYN77_000101"
     CDS             complement(22974..23867)
                     /locus_tag="VYN77_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1557646.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEISLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            24352..24571
                     /locus_tag="VYN77_000102"
                     /pseudo
     CDS             24352..24571
                     /locus_tag="VYN77_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            24986..25603
                     /locus_tag="VYN77_000103"
     CDS             24986..25603
                     /locus_tag="VYN77_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1557647.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(25646..26533)
                     /locus_tag="VYN77_000104"
     CDS             complement(25646..26533)
                     /locus_tag="VYN77_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1557648.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNVGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(26682..28565)
                     /locus_tag="VYN77_000105"
     CDS             complement(26682..28565)
                     /locus_tag="VYN77_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1557649.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGVFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGFFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            28942..30396
                     /locus_tag="VYN77_000106"
     CDS             28942..30396
                     /locus_tag="VYN77_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1557650.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDKNWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSHAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            30377..31342
                     /locus_tag="VYN77_000107"
     CDS             30377..31342
                     /locus_tag="VYN77_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1557651.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(31378..32652)
                     /locus_tag="VYN77_000108"
     CDS             complement(31378..32652)
                     /locus_tag="VYN77_000108"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1557652.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLEFANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLIGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VAFSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(32652..33824)
                     /locus_tag="VYN77_000109"
     CDS             complement(32652..33824)
                     /locus_tag="VYN77_000109"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1557653.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENADSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(33955..34203)
                     /locus_tag="VYN77_000110"
     CDS             complement(33955..34203)
                     /locus_tag="VYN77_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557654.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNRAYKATYIGELSSN"
     gene            complement(34271..34896)
                     /locus_tag="VYN77_000111"
                     /pseudo
     CDS             complement(34271..34896)
                     /locus_tag="VYN77_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(34958..35407)
                     /locus_tag="VYN77_000112"
     CDS             complement(34958..35407)
                     /locus_tag="VYN77_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1557655.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(35409..36167)
                     /locus_tag="VYN77_000113"
     CDS             complement(35409..36167)
                     /locus_tag="VYN77_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1557656.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(36282..38261)
                     /gene="pknB"
                     /locus_tag="VYN77_000114"
     CDS             complement(36282..38261)
                     /gene="pknB"
                     /locus_tag="VYN77_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1557657.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(38258..38998)
                     /locus_tag="VYN77_000115"
     CDS             complement(38258..38998)
                     /locus_tag="VYN77_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1557658.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(39013..40326)
                     /gene="rsmB"
                     /locus_tag="VYN77_000116"
     CDS             complement(39013..40326)
                     /gene="rsmB"
                     /locus_tag="VYN77_000116"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1557659.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(40319..41254)
                     /locus_tag="VYN77_000117"
     CDS             complement(40319..41254)
                     /locus_tag="VYN77_000117"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1557660.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGALFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(41267..43663)
                     /locus_tag="VYN77_000118"
     CDS             complement(41267..43663)
                     /locus_tag="VYN77_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1557661.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVVKRAYEVMNILRSSLSETSSILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(43729..44043)
                     /locus_tag="VYN77_000119"
     CDS             complement(43729..44043)
                     /locus_tag="VYN77_000119"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1557662.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(44068..44694)
                     /gene="gmk"
                     /locus_tag="VYN77_000120"
     CDS             complement(44068..44694)
                     /gene="gmk"
                     /locus_tag="VYN77_000120"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1557663.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVSLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(44824..46437)
                     /locus_tag="VYN77_000121"
     CDS             complement(44824..46437)
                     /locus_tag="VYN77_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1557664.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(46628..46882)
                     /locus_tag="VYN77_000122"
     CDS             complement(46628..46882)
                     /locus_tag="VYN77_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEV1557665.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGACSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(46886..47140)
                     /locus_tag="VYN77_000123"
     CDS             complement(46886..47140)
                     /locus_tag="VYN77_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV1557666.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(47378..48475)
                     /locus_tag="VYN77_000124"
     CDS             complement(47378..48475)
                     /locus_tag="VYN77_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1557667.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRTQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLYPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLSYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(48485..49231)
                     /locus_tag="VYN77_000125"
     CDS             complement(48485..49231)
                     /locus_tag="VYN77_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1557668.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(49334..49687)
                     /gene="rsfS"
                     /locus_tag="VYN77_000126"
     CDS             complement(49334..49687)
                     /gene="rsfS"
                     /locus_tag="VYN77_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1557669.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(49700..50200)
                     /locus_tag="VYN77_000127"
     CDS             complement(49700..50200)
                     /locus_tag="VYN77_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1557670.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(50201..50794)
                     /gene="yqeK"
                     /locus_tag="VYN77_000128"
     CDS             complement(50201..50794)
                     /gene="yqeK"
                     /locus_tag="VYN77_000128"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1557671.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(50794..51423)
                     /locus_tag="VYN77_000129"
     CDS             complement(50794..51423)
                     /locus_tag="VYN77_000129"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1557672.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(51475..51786)
                     /gene="yhbY"
                     /locus_tag="VYN77_000130"
     CDS             complement(51475..51786)
                     /gene="yhbY"
                     /locus_tag="VYN77_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1557673.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(52001..53107)
                     /gene="yqeH"
                     /locus_tag="VYN77_000131"
     CDS             complement(52001..53107)
                     /gene="yqeH"
                     /locus_tag="VYN77_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1557674.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            complement(53110..53700)
                     /locus_tag="VYN77_000132"
     CDS             complement(53110..53700)
                     /locus_tag="VYN77_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1557675.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRIQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(53652..54560)
                     /locus_tag="VYN77_000133"
     CDS             complement(53652..54560)
                     /locus_tag="VYN77_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1557676.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(54617..55177)
                     /locus_tag="VYN77_000134"
     CDS             complement(54617..55177)
                     /locus_tag="VYN77_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV1557677.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            55316..56534
                     /gene="sstT"
                     /locus_tag="VYN77_000135"
                     /pseudo
     CDS             55316..56534
                     /gene="sstT"
                     /locus_tag="VYN77_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            56602..57555
                     /locus_tag="VYN77_000136"
     CDS             56602..57555
                     /locus_tag="VYN77_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1557678.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAISVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYGQ"
     gene            57699..58079
                     /locus_tag="VYN77_000137"
     CDS             57699..58079
                     /locus_tag="VYN77_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1557679.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            58178..58399
                     /locus_tag="VYN77_000138"
     CDS             58178..58399
                     /locus_tag="VYN77_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1557680.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            58416..58730
                     /gene="trxA"
                     /locus_tag="VYN77_000139"
     CDS             58416..58730
                     /gene="trxA"
                     /locus_tag="VYN77_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1557681.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            59036..59569
                     /gene="queF"
                     /locus_tag="VYN77_000140"
     CDS             59036..59569
                     /gene="queF"
                     /locus_tag="VYN77_000140"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1557682.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            59631..>59802
                     /locus_tag="VYN77_000141"
     CDS             59631..>59802
                     /locus_tag="VYN77_000141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557683.1"
                     /translation="MFEVVDKTDEVSLKHCFEVVDKTDEVRSKHCFEVADRTDEVRSK
                     HCFEVVDRTDEVR"
BASE COUNT        17064 a        13576 c        11394 g        17768 t
ORIGIN      
        1 ttgtcctgtt cttgtttcat tttactatat ttccatgata aaacgcatag tatcaagttt
       61 ttttaatccc ctgatactat gcgtgtttgt aatttttaag attttgtgct tagagtcgac
      121 tccttatttt agatatttaa aaggaatctc acttccacag agccagttgt agacttggtc
      181 attgacaaat acattcatgg cttcgtgagc atactcaggc atgatgcgat aggttttatc
      241 gcaggtcaga cgattataaa tcgcaaactg ggtaatggga tagcaaacat cgtcgtccaa
      301 gcccgtaatc atcttaacct caccttggat acgatgggca agatttttga catcgatata
      361 ggcaagggtc gccatgattt cctcctctgt ttcatggaag gggtcgtgaa acttgaaata
      421 acggaaaagt tcgtcgtaag cctcgctagt attaccaatc tcaatcaccc gtctgaagtc
      481 tgacaagaag ggataaacag caactgtttt ctgaattcga ggattgagcg ctgctgcaac
      541 tagggctaga gcccctcctt gtgaggcacc atagctagaa agacgcttct catcaacctg
      601 agacagacta gcaacaattt cgaccaactg gtaaatatcc agataaacat ccttataaaa
      661 gaggtggtcc cgaccttcca cagcaccacg gataatatgc cccttcacgg tatttcctaa
      721 aggagaacgc aagccgtctt gtgagtaacc tgactggccc cgcacatcca tggaaacaac
      781 accgtaacca gctacggtga agcccagcat gtcggcccag tcccagccac gtcccatata
      841 accatgaaaa tggaagatta gtggaacctt ctcctcactc tttggaagaa caatgcgtgc
      901 atagaccttt ccttccttgc ttccttcaaa tgttaactca tagcacttga cttgaggaat
      961 gtggaaatct ctttcctcca agtggtagga tggaagcgtg gaaacatttt tcacttcccc
     1021 atcccagaaa gcatcaaagt cttccggaac ctcatccctt cctctatagg tcttaatttc
     1081 ttctagcaaa gctggatttt tcatagcatg ctccttttat tttgtaatcg ttaccacaac
     1141 tgtagcatat ctatgtaatc aatgcaagaa agaaggataa atagaaagtg aatttagatt
     1201 ctttactttt aggatgatgg gctgaaagta gttttagaaa cgaaaaagag catttcgctc
     1261 tctcttctgt ttatactagt tttttgcttc cttcttttgg aaagtggttt ggtaatttac
     1321 tttaatcgtt actgtcatat gagagtcctc gtttcttttt gatgacttta gtataagagc
     1381 caaacctaaa aactagctta agttttcctt agagaaagct taatccagat gttctttctt
     1441 ttccagatga agggcaatca tgcgccactc tggatcttct tgccaagctt ctatagaact
     1501 aatgtagcta gcaacctttc ttgcttcttc taaaatcgga aaatcttcga taatatcagc
     1561 cacttggaac tctggaagtc ctgactgtct ggttccaaaa atctcaccag aaccacgcat
     1621 tttcaaatct tcctccgcaa ggacaaatcc attggtcgtt tctgtcatga tgcgcatgcg
     1681 gtctttccca gaatccgtct tgggattggc aacgagaaca gcgtaggact gcttgtcccc
     1741 ccgaccgaca cgacctctaa gctggtgaag ctggctgagc ccgaagcgat cggcatccat
     1801 gataatcatg acagtcgcat tgggaacgtt gaccccaacc tcaataaccg tcgtcgaaac
     1861 cagaatatcc gtctttctct ccttgaaatc ctgcatgatc tggtcttttt cgtcactctt
     1921 catcctacca tgtagaagag ccacctctgc cttgcctgca aaatgagccg tcagctcttc
     1981 tgataaggca atggcatttt tcaaatctag agcttctgat tcttcaatca aaggagagat
     2041 gacataggct tgggaacctt tttgaatttc cccctctaac caagtcaaga cctgaggtag
     2101 ttgctcatgt ttgatccagc gcgtcacaat aggcttccga cctgctggca tctggtcgat
     2161 aatggaaaca tccatatctc caaaggctgt gatggcaagc gtccgtggaa tgggagtcgc
     2221 cgtcatcatg aggacatctg gattgtcacc tttttcccgt aaaatacgcc tttgccctac
     2281 accaaaacgg tgctgctcat cgataataat caaaccaaga cgagcatact ccaccccatc
     2341 ttgtatcaga gcgtgagttc ctataatcaa atcagcctca cccttggcaa tggtctccaa
     2401 gacttctctc ttttctgcag ctttcaagga acctgtcaag agagccagtt tcaaattggg
     2461 aaaaaggttc tgtaaactct caaagtgttg ctctgcgagg atttctgttg gtaccattag
     2521 ggcagcctga taacctgctg tcactgccgc aaacatggcc aagccagcga ctaccgtttt
     2581 tccactcccc acatcccctt gtaggagacg attcatgtgg tggtcggact tcatatcagt
     2641 taaaatttcc tgcaaactct tttcctgagc ttgggtcagg gcaaaaggaa gacttgcttt
     2701 aactgctgtc actttttcct gagaccaatc cagaaccaga ccacttccct gaattctatt
     2761 ttcagacttg agcgtctgca gctgcatttg gaaataaaag agttcctcaa actttatacg
     2821 gcgaagagcc tgcttgtatt ctgccaaatc ctttggaaaa tgcatagctc ggactgcctg
     2881 acaacgggac atgagtttgt atttgtctag taaagactgg ggcagatttt cttctatcaa
     2941 gaggtccagt ccctgatcaa aagccgtctt gatgacctta accagactgg cctgactgat
     3001 tccctgagcc agacgataaa caggctggag gtcatcttct acctgagcca gaaccttcat
     3061 cccagtcaga ctagccttag cgcggtccca ttttccaaag acagcaaggg ttgctcccaa
     3121 ctctatttta tcagccagat agggctggtt aaagaaattc accgcaaaaa cgacctctcc
     3181 ctgcttgaga ctaaaacgca ggcgattgcg cttgaaacca taatactgga cactagcagg
     3241 agtcactacc tgaccagaaa gaactgcctt ctcaccgtct tctagttcca gcacctgctt
     3301 ggttttgaag tcttcataac ggaaaggaaa gtagagcaag agatcttgca agttttcaat
     3361 tcctagtttg gcgtattttt ctgctgactt tggtcccaca ccaggcaaga catgcaaggg
     3421 ttgatgtaga ttcatgctcc actcctttct tttctaataa tattctctcg gaatacggtc
     3481 gctgagaagg caaaccacct catagttaat ggttacgcgg taggtcgcta cctgagttgc
     3541 agtgatttcc ttatccccat tggagccaat caaggttacc ttggttccga gtgggtaagg
     3601 cttaggcaat cgaatagtga tttggtccat cgaaaccctg ccgacaattg ggcaagcttg
     3661 gccatctacc aagacagaga aattttgcat gtctcttgtc catccatctg catacccgat
     3721 tggcacggtc gcgatgactt gctcgctatc cgcttgataa gttgctccat agcccatgca
     3781 agcaccagct ggaactgtct tgatatgaac cagagcagac tccaaggtca aggccggtat
     3841 caaatcataa ggcaaatcca agaccgctcc acttggattg aggccataca tggcatctcc
     3901 catacgaacc gcattgaaaa tagtctctac atgccaaaga gtcgttgcag aattgctagc
     3961 atgaaccagc tctggaactt ccttcatact agctaaaata gttttaaacc gttctaactg
     4021 ggcattaaaa tagtcatctg attcctcatc agcagtagca aagtgggtaa agattccttc
     4081 aacacgaaca ccgtgttgtt ggagcaaatc ttgagcctgc tcaacctcac tggcctctct
     4141 aaaaccaatc cgtcccatcc ctgaatcaat cttgaggtgg actgtcaatc cagttaggtc
     4201 cacttcctta tctaagagtg cttgaatcca ctccagtcca gccactgtca aggtgaagtc
     4261 atattcttta gctagagcaa cagcttcgat ttcagaaact cctaaaatga ggattggctt
     4321 gctgagtcca gcttgtctga gttcaatggc ttcatcgata ttggaaacgc aaaagccatt
     4381 aacatcatct tgaattgcct tggcaacggc aacagctcca tgaccataag cattggcctt
     4441 gaccacagcc aacttgagcg ttccttgagg gatatgagcc cccatttgct gaatattttg
     4501 tcgaatagct cccagatgaa tcagagcctt ggttggtcta tgtggactag ctttcatgat
     4561 tttcctccaa aatgacactg gctgtcacaa actgatcggt gtggctgata gacagccaaa
     4621 tctttcctga aaatggtgcc tgactaaaat aaggcgcccc acgttcattg ttcaagactt
     4681 ccaaatcctg aaaaccgagc ttgctaatgc ccgttcccat agccttggaa aaggcctcct
     4741 tagccgacca gcgaccagct aaatattcta tttgcctgcg tcctttgaga ctggtgaagc
     4801 gctccatttc ctgagcggtc agtacacgct tagcaaatcc ttcatgtcgt gtaactgcgc
     4861 tttctatcga agccaattct tcgatgtcaa ttccgtgtcc aactatcatt ctcatcaaaa
     4921 ggagttgaga ttccccaact ccatcctttt cacttatttt gtcaaggtaa cataaatctc
     4981 ttctaccaag gcctctgtat tttcccaacc taggcaaggg tcagtaatag agcaaccaaa
     5041 gatctctggt tggttttgac gaccatctgc taggtaagat tcaatcataa atcctcgaac
     5101 cgtcttttta attttctcat tccaatcacg attctgcaag gtctggcgaa caattcgaat
     5161 ctgctccata tattgcttgc ctgagttatc atggttggtg tcaatgagga taaaaggatt
     5221 ttcaagtccc atggtttcat agcgttcaat ggcttggagt aggttttcat agtagtaatt
     5281 cggcatataa ttgccatact cgttgactgc tccacggagg ataacatggg ccaaaggatt
     5341 acctgatgtc tcaacttcct gcccatgata aaggaaggtt tgcttgtttt gagcagcata
     5401 gatggcgtta aacataacac ccaaatttcc tgaggttgga tttttcatcc ctactggtgc
     5461 atcaatccca gaagccacaa agcggtgctc ttggtcttcc acagaacgag ctccaacggc
     5521 atggtagctg accaagtcat ccaccaagat cagatttgac ggataaagca tctcatctgc
     5581 cgttgtcaaa ccagtctctg taatcacgcg gtagtgcaac tggcgcacag cctgcaagcc
     5641 gttaatcagg cttggagcct tagaagtatc tggctggtga accaatcctt tatagccgtc
     5701 tccattggta cgaggcttag cagtatacac gcgcatgacc atgaaaatct tgtccgctac
     5761 cttcttttgc aaggcggata aacggcgagc atattccaag accgcctctt cattatcaga
     5821 agagcaagga ccaatcacca agagaatacg gtcatcttcc cctgaaataa tatctgccaa
     5881 ttccctgtca cggctttcct tgagtctcaa ggcttctgca gacaattggg tttcagcctt
     5941 gatgacttcc atatcgattt cttgaccttt ttcaataaat gccatcttat tctcctaacg
     6001 ttttgtaaat ttctctgaca agagcttctg tgttatccca acccaggcaa gggtctgtga
     6061 tagacttacc aaatacttct ggctcatttt gtcgaccatc ttccagataa gactcaatca
     6121 taaaaccacg aacgaactgc ttaatttttt cattccaagc acggttaatc aaggtctggc
     6181 ggacaattcg gatctgttca atatactgct taccagaatt gtcatgattg gtatcaatga
     6241 tgataaaagg attttccaag cccattttct catactgggc aatggtatca attaaattgt
     6301 cataatagta gttgggaata ttttttccat actcattaag agcaccacga agaatagcgt
     6361 gtgaaagcgg gttcccagtt gtttctactt cttttcctaa gaaaaggaaa ctttgtttgt
     6421 tttgagcagc ataaatccca ttaaacatga cattgagatt tccagaggtt ggatttttaa
     6481 aaccagtaga aaatcctgcc ccacttgcca caaagcggtg ttgctggtct tcaactgaac
     6541 gggcaccaac tgccatgtaa gaaatcaaat catctacaag cggaaggttt tcaggataaa
     6601 gcatttcatc agcagttgtc atccctgttt ctgtgatgac acgatagtga agatggcgaa
     6661 cggctttgat tccattgata agactaggcg cttctgtcgc gttaggctgg tgaatcaagc
     6721 ccttatagcc atctccgttg gtacggggtt tggcagtata aacacgcata accataaaga
     6781 tacgatctgc cacttcttct tgtaggactg ccaaacgctt agcgtattca aggacagctt
     6841 cttcgttgtc agatgagcat ggcccgatta ccaagagaat tcgctggtct tctccacgta
     6901 taatggcttc tagctcttga tctcgctgtg atttcctctc caaagcctga ccttctaatt
     6961 ttgacaaggc acgaacttct tctatattta ttttagagct ttttgctgta aataccataa
     7021 ctcatctcct tataaagcaa acggataccc agtaaaaatt tacttttcac aagatatccg
     7081 cctctaaact atctcatttt attgtctttt accgtgacag tttttaaatt tcttaccaga
     7141 accacatggg caaagttcat tgcgtccaat ctggctcaaa tccaaatctt ctggcatact
     7201 tgcttggtga gcagcgatat tgcgagtcgc tgttgtactg atatgacgtt ctgcctgtgg
     7261 tctttcttgt tcatgaattt gtgctttcat catcaagcgt gtcacatcaa actcaatcga
     7321 accaatcata tcattaaaca tacggaaacc ttctgcctga tactcaacaa cagggttgtt
     7381 ctgagcatag ccacgaagtc caaccgcgtt acgcaattga tcaagggcat cgatatgatc
     7441 tgtccacttg ttatccacca ctcgtagaat caaaactttt tggaattctt taactgcttc
     7501 ttcatcgcgt agttttgaaa cctgactatc gtaaaccttc aaggcacgtt ggaaaagctc
     7561 ttccttgatg gccttatcag acaagcctga caagtcttcc atcgtaatag aatcttcagg
     7621 aagcaagttg tacttagcga agttcaaaat tgcctctagt ttctcatctt gtttagcacg
     7681 cgcatgaccg tcaacgacac gaccaatcgt gcgtttgatc attgcctgaa tttcaggtgc
     7741 caagtcacgg tctgcagtaa tgacatcata acgttgagta tagataatct cacgttgttc
     7801 acgcatgaca tcgtcgtatt gaaggacttg tttacgggta tcgtagttat ttccttcgac
     7861 acgtttctga gctgcttcaa cctgacgcgt caacatgcga gactcaatgg cctcttcaga
     7921 catgttcaag cgttcaaaga ttcccttcaa gcgttcagaa ccaaaacgtt tcatcaaatc
     7981 atcttcaaga gataggtaga attgtgactc acctggatct ccttgacgac ctgaacgtcc
     8041 acgaagctgg ttatcgatac gacgactttc atgacgttct gtaccaataa cacaaagtcc
     8101 tccaagttca cgaacacctt caccaagctt gatgtcggta ccacgacccg ccatgttggt
     8161 tgcgatggta acggcaccac gttgaccagc attcatgatg atttgggctt ctctatagtg
     8221 gtttttggca ttcaagactt cgtgaggaac accagctgca accaatttct tagaaatgta
     8281 gtcactagtt tcaaccgcta ctgtaccaac caagacaggt tgaccctttt ggtaacgagc
     8341 cttaacgtct tcgacaaccg ctttaaactt agattcgata cttgcataaa gaaggtctga
     8401 gtggtcaata cgttgaacag gacggtttgt tgggattgga ataacacgaa tgttgtagat
     8461 ttcacgaaat tcttcttcct cagtcttacc tgtacccgtc ataccagaca attttttgta
     8521 catacggaaa aggttttggt acgtgattga ggcagatgtc ttggtttcat cctggattgg
     8581 cacaccttct ttggcttcaa tagcttggtg caatccatca gaataacgac gaccttccat
     8641 ggtacgacct gtaaattggt cgacaatcaa gatttcttgc tcttcgctca ccacatagtc
     8701 aatatcgaga agcatgatgt agttggcacg aagggcgtta tcgataaagt gggtcaaagc
     8761 cacgttttcg atgtcataga ggttttcaag tttgaagtag ctttcagccc tgtcaatccc
     8821 tgaatcagac aaaccaatag tcttagactg cacatcgatg atgtagtcat ctttgttcaa
     8881 agattttaca tagtggtctg ccatgtgata caactgactg gtttcaaccg cattggcacc
     8941 tgatacaatc aaaggtgtac gagcctcgtc aatcaagata gagtcaacct catcgaccaa
     9001 ggcatagtta agcggacgtt gtaccatgtt ttcagcgcga acgaccatgt tgtcacgaag
     9061 gtagtcaaat ccgatttctg agttagttga gtaagtaata tcacactcat aggcttcttt
     9121 tttctccatt ggagatttgg tagccaagtt aatccctact gacaaaccaa gccaagagta
     9181 caattcaccc atctcagtcg cgtcacgttc tgacaggtat tcattaaccg taactacgtg
     9241 aaccccttta cctgaaaggg cattgaggta taccggcatg gtcgcagtta aggttttccc
     9301 ttcccctgta cgcatctctg gcacgtcacc atggtgaaga acaatccccc ccatgacctg
     9361 aaccttataa gggaagagac ctaggacacg tttggcacct tcacggacaa ccgcaaatgc
     9421 ttcgtaaagc aatgaatcca gtgattctcc attttgataa cgttccttaa attcaactgt
     9481 ttttgctttt agttggtcgt cagtcaaagc agccatttgg tcttcgtatt tgaaaacctt
     9541 gtcagccatc ttttccagac gacggatttc tcctttatca ttttcgataa ttgtttttaa
     9601 aatattagcc atgtttttcc ttactttaaa ttccgaatat tttagaatgt tcctttaatt
     9661 ttagcacaat tactgattat tttcaaggaa gaatccccat tttgaaaagg aaaaagaggt
     9721 tagttttcaa gctaacctct ccgactttta tgactgttta attgccaaaa atcggcaaaa
     9781 tggcaaatgt gataaataag ttaatccaaa gagaaagaca gcaaatcagt ccaaaaacaa
     9841 agagactgac tttcttggac agcaaggcca tgagaatgga cagaattcca aaagctagca
     9901 agattttctg tatgatgaag atgtcaatcc ttacccccag aatcgctcta tcccaaggga
     9961 taaagaagaa agacagccag atcagcagaa ctaaaccaat ataaaccttg gaatagcgtg
    10021 taataaatga ttttagatgt gccataagcc atctcctagg attgtatatc ctttacattt
    10081 gtatatgtcc attttcgaca actaatacat ctgtacaaag gtctgaaata tcttccgata
    10141 tgtgcgacgt caggataacc agaccatttt cttttttcag gtaagataaa atgacctgtt
    10201 tggttaacct cacactcttc tcatccaaag cattcatagg ttcatcgaga aagagtatag
    10261 aaggagagga aataaaggct tgaatcaagg ccattttttg ctttgttccc aaggataaat
    10321 gacggtattc aacgtcttca aattcctgta aatcatagta ttgaatccaa taggcaattc
    10381 ttttttccgt aactttagat gaaaaatacc ttaacagttc caaattttct ctcagggata
    10441 aatgagataa aaactcgact ttttcaatta aaattcctgc atcctgaata taatgatttt
    10501 ttatcccgta gactttcccg tcttgaagaa cttttccttt gtcaagctta atatagccag
    10561 caagtacctt taaaagaacc gtcttgccag atccattatc acctttaagt ccataaattt
    10621 ttcctgattg aaacaccaaa ttcaactgat ttaaaatagg tcttttccca taaattttct
    10681 gtccatttat aatttcaagt ctcatcgttt atcctttctt aaatttctct tccaagcgac
    10741 taacaatcaa cataactaat aaacttaaga gtactaaata cactgttaca atatgatcca
    10801 tcatccattc ttgtcccaca aatactaaaa aatagagaaa aaaggaaaaa ccagcgcccg
    10861 cactaatcag tgctatcaga taagacagta gtaagtacaa catcattaac tgagcaagta
    10921 aaaagaaaag tgcgactgtt ttgaaatgaa ttcctagcgg aagtaaacca aggaaccaga
    10981 caaacaaatc ttgagtaaaa aatttagaaa tgacaaccaa tcgtttcctt gtataatcga
    11041 aaaaggagct tgattgatag cgaatcaagt gatggtaatg gtctgccaaa tcactttcaa
    11101 gtaaaatgac agagagccat cctaccatga aaagcgaaag gtttaacaag agtttgggga
    11161 tttctaaatt cgcttcagaa tagtcattat aggaaaaaat tgaaatcaat tctctcttga
    11221 aaaagataac agcatcctgc ttttgaaccg ctgttgacaa tagatggttt atcaaaatca
    11281 tcatcagaac tatacgaagt agtaaaacaa tctttctctt tagcaaaata ttgttaaaat
    11341 tcttaaacat tatcttcata tttttcaagc accatgtaag ggacaatata aaggatataa
    11401 ggagtaaaga gtttcatcaa acttacatcc ccatagctag cttgcatcaa actcgtcata
    11461 ggaaccatat agtaaggcaa ggcactatag agcagcatag aaccaagcca aagaaacatc
    11521 aaataggtga ttgccgaacg agtcacattc cccacataat caactatttg taaaaaatag
    11581 attgcattga tgaaaatacc gattagcagg acacaagtaa agaacaaata gctttttatc
    11641 tctccatcta ggagtctttg taaaccactt ccatcagaaa atagagaatt ccatccaaga
    11701 ggagaaaaag tcccaaggaa ataggttata attgcaattg tcagcacagt cactaaatat
    11761 atcaaacaag ggatacttgc aacttgcaaa gtcaactttc tttttaaccc tggatagctc
    11821 acttctcttc caatactcaa tcgtaaaact ttatttttca gctcaatata ttgaaaacct
    11881 agcaaaacaa tgatgattgg aataataaat gattgataaa aacctagatt gaactggaga
    11941 atacgaaaat ctaatgctga ttttaataag tcatttttaa ttgcttgtcc atcgaattta
    12001 gctagatcgg gcgcatgaat atcaaaaaag aaataagtca tcaaaacgtc acgataaacc
    12061 aaagcaagaa aaagcaaagt aagcaagaca taaacaaatt tatgccttgc caaagttaag
    12121 agaggaatct tcataaatta tacctacttt acgaattaat ttggtgacca actacctgaa
    12181 atggcagctg ataaatccca ccaattttgt cttgccatat ctgctgcata aacatagcca
    12241 accgttgccc aattaccatg agttgaacga gtaccacagg ttgttaacga ttctccacta
    12301 cgagcttctc cattagaatt tactatacga tgcttaacag tcatgggatg taaatttcta
    12361 caaggattta agttcatgat atattccgca ccttcatttt ctttagtcaa ataacctgtt
    12421 ctcttataac tggtagtaat aacaaaatta ctaaaagcac ctatctgatt tgcttctacg
    12481 aaagtagttc caacagcact tatcattaaa acaggaataa ccattttcaa taatttctta
    12541 attttcattt tttctgtttc ctttcatttt ttattcaaga agattcctta taacttctat
    12601 tttaaatata gacttattct aaacgatggt atatcataca aattcctcct ttacttatta
    12661 tatatagact ctaatttctt tatccccaaa accatgtttc aaaccagata agttgaatcc
    12721 aactccaaat caaaaatcca gagtttttcg gtaaattctt caaatatttt ttaaagccca
    12781 tgactttttc cctccttttt gatataataa taccaccttt ttctacgttt tttataaatg
    12841 tagaaaatat tcacatttac ctaggaggaa caatatgaga tgggattatg gacaaatttt
    12901 taaagaaatt cgaaagtcaa aaggattgac ccaacaagat gtatgtggac aagtcataca
    12961 tcgaacaact ctaacaaata ttgaacacgg taaagttatt cccagttttg aaaacatggt
    13021 atttcttctt gaacaaattg atatgagctt ggcagaattc aagtatatat gcaacgaata
    13081 ccatcctagt aaaaggcgag atattattgt agagagccaa aatccgtcta cttttcaaga
    13141 tactagaaaa atggttgaac tcactgagaa atgtcaaaaa tatcttaaga cacatcacga
    13201 tgttcctatt caaaatatct atcgtcatac aaaaattgtc acagagttac gaactaaagg
    13261 attcaaaaac aaccacgtct tgaaagattt gtatgaagaa atttgggact atcttgaacc
    13321 tatggataca tggtacatta gtgatttgaa attgcttgga accattctct ttttctttcc
    13381 ttctgaaaat cttccccttc ttattgatag aattatgaaa accatcgaga aatataaata
    13441 cttccgagaa acaaaagcat ttttatcatc ttttttagcc aatctctcca ctgtatattt
    13501 tcaacatcat ttattcaaag aatgtgaaac aatcacccta caacttttgg tattagcaga
    13561 agaattaaaa atatacgata ttcttggatt ctctcaagta cgcttgggaa tactacaaca
    13621 taactccgat ttgattgata aaggaataac tttattacga ctaaccaaag aagaggcatt
    13681 agttaaaata ctcgaaaaag aaattaatga tttttcaaac ctataatttg taataactct
    13741 catcatttta ttatagtaga ttgtaataga atgtgaacga atcaattagg ggatctaaag
    13801 cattttatac caatgtttta taagttgtat tgtattattc catattcaat ccactataca
    13861 agatgaatat ttctctcaag tttcataatc aaaataaaaa tgactgtatc tatagctcca
    13921 taaacaaatc atagatacag tcatttttta gttcctacac cttcctttat gcacaccttt
    13981 agatttccct agttcccgcc tcaagcgaat catttttttg aaaaagacat aggctaacca
    14041 atcactagca aaagcgataa gtagtaataa tagccaaata ccccataact tgatatctgt
    14101 caaatttctc aagacgatat tgaaaaacag aactgaaaaa actgtccaag caaggctaaa
    14161 aagagcatag agggtgatgt aaatccagta aaaatagtaa aaaattgaga aaatcttact
    14221 atctctgttt gccctttcaa tccagctgaa aaagtaaaaa cagggtaata agattatcat
    14281 tttaaacaag tgttccatca caaacctcct cttttaaatt gttttcttta gtttcgttct
    14341 attaaatgtt attaccaata taaaccttgg aatagcgtgt aataaatgat tttagaggta
    14401 ccataagcca cctcctacaa atagaaaccg atataaatca atgccttcca cccttagact
    14461 tccctagttc ctgtctcaag cgaaacattt ctttgaaaca ggaataagtt aaccaattca
    14521 taccaatagc tagcagaata aaaagaaacc aaatgcccca taacttgata tctgtcacat
    14581 ttctcaagac ggtattgaaa aacagaactg aaacaactgt ccaagcaagg ctaaaaagag
    14641 aatagaaggg gatgtaaaac cagtaaaaat aataaaaaat tggaaaaaac ttactatttc
    14701 tgttggcctt ttcaatccag ttatcaaaat aaaagtacgg tgctaaaagt aagaatttaa
    14761 acaaatgttc catcaccgac atcccccctt cttttgatag cgttttctat tattttatta
    14821 tatcaaaaaa atccggaact gtcattccag attctacttt tttatttgcg ttttcttgcg
    14881 atgagatgaa tcggtgttcc ctcaaaaaca aaggccttgc ggatttgatt ttccaagaaa
    14941 cgcaggtaag aaaagtgcat gagttcttct tcattgacaa agatgacaaa ggttggtggt
    15001 ttggttgcca cttgggtcgc atagaaaatc ttgagacgtt ttcctttgtc tgtcggtgtt
    15061 gggttgatgg caatggcatc catgatgaca tcgttcaaga cagctgatgg aatacgtgta
    15121 ttttgacttt cgctgatttg cttaatcatc tcaggaagtt tgtggagacg ttgcttggtt
    15181 aaagctgata caaagataat cggtgcgtaa ggcaggtatt ggaactgctc acggatatct
    15241 tcttcccagt ttttcatagt gtggttatct ttttcaagcg tatcccactt gttgaccacg
    15301 ataatcatcc ctttaccagc ttcatgagca aatccagcga tacgcttgtc gtattcacga
    15361 atcccctctt ccgcattgat gaccattaag accacatctg aacggtcaat agcacgcatg
    15421 gcacgcatga cagagtactt ctcagtattt tcataaacct taccagactt acgcatacca
    15481 gccgtatcaa tcatggtaaa ctcttgacca tctgtatctg taaagtgggt atcaatagcg
    15541 tcacgagttg ttccagcaac aggactggca atgacacggt cttctcccaa gatagcatta
    15601 atcaagcttg attttccaac gttaggacga ccaatcaagc taaacttaat gacatctgga
    15661 ttttcttctt catattcatt tggaaggttt tccacaatcg catctagtac atcccctgta
    15721 ccgataccat ggacagatga gataggcagt ggttcaccca aaccgagggc atagaaatca
    15781 tagatatcat ttcgcatctc agggttgtcc accttgttaa ctgcgaggat aactggtttg
    15841 tgggtcttat aaagcttacg ggctacgtat tcgtctgcat cggtaattcc ttccttaccg
    15901 gacacgacaa agacgataac atctgcttct tccatggcaa tttctgcctg gtgcttgatt
    15961 tgttccatga aaggagcatc gacatcatca attcctcctg tatcaatcat gctaaaagaa
    16021 cgattgagcc actcacccgt tgcataaata cggtcacgtg tcactccttc gacatcttct
    16081 acaatggaga ttcgctcacc agcgatccga ttaaataggg ttgatttccc aacattggga
    16141 cgtcctacaa tggcaatagt tggtagggcc ataatttctc actttctaca ataatttctt
    16201 ctgttcaaga ttttttctag ttgagcttgg ttcagcttga ccaaactgtt ctgctagacg
    16261 ctgactccag cttgtggtcg cacgcgcccc agcatagtca gcctgaacac ggtcataagc
    16321 ttggattgcc tcagttgact gttcttggta ttcttcctca aagactacat tatctagtgg
    16381 cagtcttggt ttcacatcat gagtttgatt tggaacacct agtgccatcc caaagacagg
    16441 ataggtgtag tcaggcaggt taaagagttc tgccacttct tctgacttgt accgtaccaa
    16501 gccaataatc acaccaccat aacccaaact ttcagctgcc agcagggcat tttgtccagc
    16561 caaagccgca tccaccgaac taatcaagag accttctaca ccttggggtt ggaaggtgtc
    16621 ggtatgaagt cgggctccct tttctgctcg gttcaaatct ccgacaaaga gaaggaaaac
    16681 agcagactgg cgaatggctt cttgaggtac caattcatac aaggcatctt tcttctcttg
    16741 acttcgtacc acaatcacag agtaggattg gaaattcttc caagatgatg ccatctgggc
    16801 tgctgtcaaa atctcattta agtctacttg gggaatttct tgctctttaa acctgcgcac
    16861 tgaagtatga gccttcatca atttaatggt ttctgtcatc gacggtttac tccttctaaa
    16921 cgagtctcct cagccaaata acggatgcgt tccatgaccc gtctggcttc ccaggtttcg
    16981 tcatttccat gtttcacttt cgcaaaatgc ttctccaaat cttcaaagtt gaagttggat
    17041 gtgaaaaagg tcggtaaatt ttcctgcatc cgatattgga gaatgacctg caggatttcg
    17101 tcacgcaccc aaacggttga ttgctcggcg ccaatatcat ctaaaatcag gacctcagac
    17161 agcttaatct catccaccaa ggtcttaaca ttgccatcac tgatagcatt tttgacatca
    17221 atgacaaagc taggatagtg gaggagagtt gatgaaacac cacgtttttc tgataaatca
    17281 tgagctaagg ccgccaccat gaaactttta cccacaccaa agtctccata taagtaaaga
    17341 cctttcaaat tattggggta atcgttaaca aattctatca ttctttttag aacagttaag
    17401 cgctgaacat catcacgata aacatctaaa aaactgacat ttttcaggct agatggaaaa
    17461 ttaattaagt tgagacgttt cttgatagcc gcttcttttt cagctgcgat tagttcagga
    17521 gtttcttcat aagaaacatc cgcatagcca tggttcatga ctaatatagg cttgtagcct
    17581 ttggcaatat aatctgtatc tcctcggagg aatttatctc tctcagtgat gtattggtta
    17641 aacttggaaa tactacggtt caactcgtcc tgattgaggg attcttgctg gacaaaggcc
    17701 gcaacatcag gatccttcat gattttctga actaaatctt gatagtgaaa acgacttggt
    17761 tgacgtttga gtacatctcc gacactttcc atctaatcac ctccgttatc taattttgct
    17821 aataattcta gggtttggcg ttccatctct tctaattcct cagcggatga cgtattttta
    17881 tattcaggat tactccattt aggaatattg gtttttgctg gggcagtctg attctgtttt
    17941 tgtgtttttg ctttctgtcc tcgctcacgg atgcgcaaga ctgcctcttc tgccgaatga
    18001 atcttttgat aggcatagtc attggctacc ttcatggcat atttctcatt gatatttgcc
    18061 gaatcgacct tattaaaggt caataaaaga atgatattga tgacttcgtc cagcaagccc
    18121 aagccagcca tctgttgcaa gagttctctt tctgtttggg taatggttcc cttgcgtgtt
    18181 tgcttgattt ctgccaagaa ctgcagggca gttttacttt tggcttcttt gataatggtt
    18241 gtttccttaa gactaaagtc agaggaaact ggtttttgag caattttttc acgcatgcgt
    18301 ttggttgaaa taacctgaga aacagctgtt gacttggcca attgataggt ttcaaaccaa
    18361 gtccatttct tctcctcggc aatagcaaag aggtttaaga catcggactg ctcatccgca
    18421 aaacgaagtt catctcgagc catcagctgg cgaaaatgtt ccaagtcaaa atcattggcc
    18481 actttcttct tgagaccaag gtcttcttga ctgcctagtt ctgccaattc tggaaagact
    18541 tgattgagtg agacaggtat ttcttcacca tcagcacttt caactttcaa atcctccaca
    18601 gcagagtcgc caatcttttt ctctaagagt ctgcgataaa caggatgctc caagaagtct
    18661 tgactagata gaggagcatg gagggctagc tgataaacat cccccttttg atagagagtc
    18721 aagagattaa aagcagataa gattttcaat gattttatca gtctatccat cccaaagttg
    18781 agatgattga ggatacttga aaaaagatat tcctttctac cattatccca aaaactgatt
    18841 gtataaagat aaaggctcag tgcctcctga ccgataatcg ggaggtagca ctgtaccaga
    18901 gatgaggtat cttgcgacac ccgattattc tttagataag aaaaacggtc aattggcttc
    18961 atttatcttt cctttttctt tttagaggac tgggtgattt gttggagcaa gctctctaac
    19021 tcactgacat ccttaaaact acgatagaca ctagcaaaac gtacataggt aatctcgtcc
    19081 aattcagcca actcctccat gacgagtgaa ccaatgtcct cactttgaat ttcattttca
    19141 tttcgaccac ggagtttctg ttcgatacga ttgactacca tgttgatttc atcacttgac
    19201 acaggacgtt tctgggctga gcggataatc ccattaaaga ttttatctct ggagaattgt
    19261 tcccgtgtac catctttttt aacaaccact aaggttcttt cttctactcg ttcgtaggtt
    19321 gtaaaacggt gttggcattc gtcgcactca cgtcttctac gaatggtgtt cccttcttct
    19381 gcttggcgac tatcgataac acttgacttg gtagccccac attttggaca acgcatcctt
    19441 tccctcctta tcgttttctt ttcattatac cattttttaa gcgattccca aaacaattct
    19501 tctttttgct tgacaagttt tttgttttgt tgtattattt aattaagaca aaagaataag
    19561 agaaaggaga ctaagatgtc ctggacattt gacaacaaaa aacccatcta tttacagatt
    19621 atggagaaaa tcaagcttca gattgtttcc catacactgg aacccaatca acaacttcca
    19681 accgtgaggg agctagctag tgaggctggt gtcaatccca ataccatcca aagagcctta
    19741 tcagaccttg aacgagaagg atttgtctac agcaagcgaa caactggacg atttgtgact
    19801 aaggataagg agctaatcgc ccagtcacgc aaacaattat cagaagaaga attggaacac
    19861 ttcgtttcct ccatgaccca ttttggctat gaaaaagaag aactaccagg cgtagtcagt
    19921 gattatatta aaggagttta agcctatgtc attactagca tttgaaaatg tatccaaatc
    19981 atatggagca acaccagccc ttgaaaatgt ttctcttgac attccagctg gaaaaattgt
    20041 cggccttctt gggccaaacg gctcaggaaa aacaaccctg attaaactaa ttaatggcct
    20101 cttacaacca gatcaaggac gtgtcctcat caacgacatg gacccaagcc cagcaaccaa
    20161 ggccgttgta gcttatttgc ctgatacgac ctatctcaat gagcaaatga aggtcaaaga
    20221 agccctaacc tacttcaaga ccttctataa agatttcaat cttgaacgcg ctcatcatct
    20281 acttgcagac ctgggcattg atgaaaatag tcgtctcaag aaactatcaa aaggaaacaa
    20341 agaaaaggtt caactgattt tggttatgag ccgtgatgct cgtctctatg ttttggatga
    20401 acccattggt ggggtggatc cagcagcccg tgcttatatc ctcaatacca ttatcaacaa
    20461 ctactcacca acttctaccg ttttgatttc tacccacttg atttctgata tcgagccaat
    20521 cttggatgaa attgtcttcc taaaagacgg aaaagtcgtc cgtcaaggaa atgtagatga
    20581 tattcgctac gagtcaggtg aatccattga ccaactcttc cgtcaggaat ttaaggccta
    20641 agcaaaggag attatttatg ttttggaatt tagttcacta cgaatttaaa aatgttaaca
    20701 agtggtattt agccctctac gcagccgtgc tagtcctttc tgccctcatc ggaatacaga
    20761 cacaaggctt taaaaatcta ccttaccaag aaagtcaggc tactatgcta ctttttctag
    20821 ctacagtctt tggtggcttg atgcttacac ttgcgatttc aaccattttc ttgattatta
    20881 aacgcttcaa aggtagtgtc tacgaccgac aaggctatct gactttgacc ttgccagttt
    20941 ctgaacacca tatcatcaca gccaaactaa tcggtgcctt tatctggtca ttgattagca
    21001 ccgctgtatt ggctctaagt gctgttatta ttctggcttt aacagctcca gaatggattc
    21061 ctctttctta tgtgattaca tttgtagaaa cacatctccc tcagatcttt cttacaggta
    21121 tatccttcct actaaatacc atttcaggaa tcctctgcat ctacctggct atttccattg
    21181 gacagctttt caatgaatac cgtacagcac tcgctgttgc agcctacatt ggtatccaaa
    21241 tcgtcattgg atttattgaa cttttcttca atcttagttc taatttctat gtcaattcac
    21301 tggtaggact caatgaccat ttctatatgg gagcaggtat agccattgtt aaagaactca
    21361 cattcatagc tatcttttat ctcggaacct actacatctt gagaaataag gttaatttgc
    21421 tttaaataat ttttacctag atatgtaaca tactcataga acaaaagaga ccaggcaaaa
    21481 agtctttaaa attagaaaac gcatagtatc aagtgttaaa aatccttgat attatgcgtt
    21541 ttttgtgagt tgacttattt cctttcacta tatcgcaaaa tgaaataaga acggaacgat
    21601 gggattttgg aattcaaatc aatttgtaag aatgttttag aagtaatatt atcctattcc
    21661 agattcagtt cactatacaa ttgagttttc aagcaacttg tttacataat gtgtacataa
    21721 ttaggttcgt gattccaccc ttttcacctt taaaaacctc gctttcgcaa ggctcttcta
    21781 tttataagat aaggcacgtt taaaggtttt ccaaatccct aaatcatccg tttgaagaac
    21841 gagactagca tacatgcgtc cgataaatcc tgttgctacc accgcaaaaa tcactgtaat
    21901 agcaagtgaa atccatgctt ccgctccccc cgcatagcca ttaatcgttc gaaacggcat
    21961 aaagaaggtc gaaataaagg gaatataaga accaatcttc aagaggagat tgtcaccagc
    22021 tgcacctaga gctgtcactc caaaaaaacc acccataatc aaaatcatca aaggcgacaa
    22081 ggctttccct gagtcctcag gacgagaaac catagatcct aggaaggctg ccaagactac
    22141 atacatgaaa agactgatca aaataaagag cagggtattc agtgagatag catctcccaa
    22201 gtgatccaaa ataccagact gagccaagaa tggcaaatct ttaaagagca aaacggcagc
    22261 cagaccacct acaacataga tcccaatatg cgttaaaatc actagaaaca gagccatcat
    22321 ccgcgcatag aaatagtgac ttgcccttat gctagaaaaa acgacttcca taattttggt
    22381 gcctttttca ctggcaactt cctgagctgt tacacccgca taggtaatca gaatcatata
    22441 aagaaagaat cctaaggcac ctgctgcaat tgtttgaata aactttttat tttccttggc
    22501 ttcatcaatc ttttctgtga attgaattgt ctgcgctaag cgtttttcct gctcttgaga
    22561 caaggaagca gttgaacgat taagctgatt ttgcagttca ttgagtgtac ctgtaacctc
    22621 aaatttaatt ccattttcaa gcgatgtttc gccatgataa actgccttta gaacactatc
    22681 ttcttgatca atggtcaaat aaccttttaa tttttcttct ttaattgctt ctttggcact
    22741 tgcttcgtct ttatagtcga agttaacacc atttacattc ttcagtcctt ctgctacaga
    22801 tggcactgtt gtcactactg ccactttatt atttttagcc atagaagaac cttggagatg
    22861 cccaattcct acagagattc ctaaaaagag gaacggcgaa atcaccataa agaagaaact
    22921 ccatgactcg acatgtcgaa gataggtttc cttgattaca acccacatat ttctcatact
    22981 tccactcctg attctagttt aaagatttca tcgatagttg gcgcttgttg gtcaaatgtt
    23041 gcgatatatt gaccttgagt caagattggg aagagttccc ttccagcgct ctcatcctcc
    23101 aaaatcaatt tccaactgcc ttgtttggtc aagctcacct gtttgacatg aggaagattt
    23161 tccaattctt ccttgcttcg ttcacttgaa acaaagagac gcgttttccc gtattgattg
    23221 cggacatcct gaactggtcc gtgcaagacc acacggccat ctcggatcat cagaatatcg
    23281 tcacaaagtt cctcaacatt ggtcatgaca tggtcagaaa agataatggt tgctccgcgc
    23341 tctttttcct gaaaaatgac ttgtttgagc aattctgtat taactgggtc caatccacta
    23401 aaaggctcat ccaagataat caagtctggt tcatgaatca gagtaataat gagctgaatt
    23461 ttctgctgat ttccttttga caaactcttg attttatctg tcagctttcc tttcacttcc
    23521 aacctcttca tccattgagg gagtttttcc ttgacctcct tggcatccat gccctttaga
    23581 gtcgccaagt agcgaacttg ctcaaggact gtcaatttag gcatgagact gcgttcttca
    23641 ggtagatagc caatccgagc ataagtttcc tgacggatat cctgcccatc cagactgatt
    23701 tctccttggt attctaaaaa tttcaaaata ctgtggaaaa ttgttgtttt cccagcaccg
    23761 tttttcccga ctagtcccaa aatacgacct ggtcgtgcct gaaagtcaat accaaacaaa
    23821 acttgcttgg atccaaaact tttctctaga cttcttactt ctagcatctt tcacctccga
    23881 aatgtcttgc actcattata ctcctttttg atagccttta caatgatttc tgtccatttt
    23941 tagaagacta ttgctgtgta aaatatggcc tggagcgttt ttagtgataa atccattata
    24001 cagtagcaaa ctcaatttat cacctctgct cctcaactgt ctatttttga tcctgaattg
    24061 ttatttgagt aactcctttt tcctcataaa gttttcttcc tctaaaactt ctggaaaaag
    24121 gcgaatagtt tcagacaaca tttttataag aaacaagttc atctgtcatt tcaagaagga
    24181 gtaatccttt atttactaat ggacggaacg gaattcaact gcttgtccga tatgttttct
    24241 aaggattata tagtaaaatg aaataaaaac aggacaaata gatcgggaca gtcaaatcga
    24301 tttctaacaa tgttttagaa gtagatgtat actattctag tttcaatctg ctatatctat
    24361 tatgcacacc cctataggat ctaatgaaaa tcacaacagg ctcattcata gatggttacc
    24421 taagcctaag ggaactaaga aaacgactac caaggaagtc gcattcatcg aaaagtggat
    24481 taacaactat cctaaaaaat gcttgaacta caagtccccc agagaagact tctggatgac
    24541 taacttgaac ttgaaattta gcaataatta attcaccatc taactatatt tagtaattat
    24601 ttcagaactg attaatatta aaattaacta acgattcaaa ggattcatac tagccataaa
    24661 ttacgtccat cagagagaga ctcttactac ttttagattt tagtctttct agcttcagaa
    24721 tacatctaaa ctttagggaa aatgactatt cgaaagcgcg aatgcctcaa aattatctca
    24781 gataagctat tcgaaactta gaatgctttt aaatttatgg aattgcgatt attcgaaacc
    24841 tagaatgcat ataaccttta gttgacagac ctattctaag tctcgaaggg ctatttactt
    24901 tctattcctt atcaaaaaag actcattccc cctttctcct ccaaaatatg gtatagtaga
    24961 aatatactat ctatgaggag tttacatgtc acaggataaa caaatgaaag ctgtttctcc
    25021 ccttctgcag cgagttatca atatctcatc gattgtcggt ggggttggga gtttgatttt
    25081 ctgtatttgg gcttatcagg ctgggatttt acaatccaag gaaaccctct ctgcctttat
    25141 ccagcaggca ggcatctggg gtccacctct ctttatcttt ttacagattt tacagactgt
    25201 cgtccctatc attccagggg ccttgacctc ggtggctggg gtctttatct acgggcacat
    25261 catcgggact atctacaact atatcggcat cgtgattggc tgtgccatta tcttttatct
    25321 agtgcgccta tacggagctg cctttgtcca gtctgtcgtc agcaagcgca cctacgacaa
    25381 gtacatcggc tggctagata agggcaatcg ttttgaccgc ttctttattt ttatgatgat
    25441 ttggcccatt agcccagctg actttctctg tatgctggct gccctgacca aaatgagttt
    25501 caagcgctac atgaccatca tcattctgac caaacccttt accctcgtgg tttataccta
    25561 cggtctgacc tatattattg actttttctg gcaaatgctt tgacacgtaa aaaatccgtt
    25621 tggtttccca agcggatttt gtgctttatt ttgaaacttc ttttgcaaga acaaagttcc
    25681 caagtgtggc agaaccattt cctgcgactg ctggcgtcac gatatagtca cgcacatctg
    25741 gtactggtag gtaaccatta agaagagatg taaatttctc acggacacgg tccagcatat
    25801 gttgttgagc catgacccct ccaccaaaga caatcacgtc tgggcggaaa gtcactgtcg
    25861 cattaaccgc agcttgagcg atatagtagg cttgaacatc ccaaacaggg ttgttgagtt
    25921 caatagtttc cccacgtaca cctgtacgag cttccaaact tggaccagct gcataacctt
    25981 ctagacatcc cttatggaaa ggacaaacac ccttaaactc tttttcaata tccattgggt
    26041 gtctagcaac ataataatga cccatttcag ggtgacccac accaccgata aactcaccac
    26101 gttggatgac gcctgcaccg atacctgtac cgattgtgta gtaaaccaag ttttcgatac
    26161 gaccaccaac attgttacgg gcaaccactt caccgtaagc agagctgttt acgtctgttg
    26221 tgaagtacat tggcacgttt agggcgcgac gaagggcacc aagcaagtcc acatttgccc
    26281 agtttggttt tggagtcgtc gtgataaagc cataagtttt tgagtttttg tcaatatcaa
    26341 tcggcccaaa tgaaccaact gcaagaccag caaggttatc gaattttgag aagaactcaa
    26401 tggttttatc gattgtttcg attggagttg ttgttggaaa ttgtgttttt tctacgacgt
    26461 taaagttttc atcaccgaca gcacagacaa actttgtacc gcccgcttcc aagcttccat
    26521 ataattttgt catgataaac ctcttgtttt tattttcttt attatagcat acttcgaaag
    26581 tctaaatgtc tctatttttt agattttcct ctgtaaatct tactatctaa taaaaacgaa
    26641 caaacatgtc atttgttcgt tttcacatta gagaggattg attagatttt cacttcgatc
    26701 acagcatccc ccttcgcaac tgaacctgtt gcgactggag ctactgaagc gtagtcagct
    26761 gtatttgtaa cgataaccat tgttgtatca tcaagtccag ctgcagcgat tttgtttgag
    26821 tcaaatgttc caagaacatc gccagctttc accttattac cttgagcaac ttttgcttca
    26881 aaaccgtcac cgttcataga tacagtgtca ataccaacgt gaatcaaaac ttcagcacca
    26941 tttcttgttt tcaaaccaaa agcgtgccct gttggaaagg caattgaaac ttcagcatca
    27001 gctggtgcat agaccacgcc ttggcttggt ttcacagcga taccttgtcc catagctcca
    27061 cttgagaaga ctgggtcatt gacatcagca agagcgacaa catcaccaac gataggagtt
    27121 acaagtgttt cattttgaag agctgctggc gcaacttctt ctttttcttc agccacttca
    27181 gcttgttttg cagctgcagt tgcgtctact tcatcttcgt aaccaaacat gtaagtaaga
    27241 gcaaaaccaa gggcaaatga tacagctacc ataagaaggt attgtggaag ttgtccgtta
    27301 ccaacataaa gcattgtacc agggatgatg gtgataccat taccagtacc agcaagtcca
    27361 aggatagaag ccaatccacc accgattgca ccagcaatca atgaaaggaa gaatggttta
    27421 cggaagcgca agttcacccc gaagatagca ggctctgtaa tacctaggaa ggcagaaaga
    27481 gcagccggga aagcaagtgt tttcagtttt ggattttttg ttttaacacc aaccgcaaca
    27541 gtagcagcac cttgagctgt catagcagct gtgatgatag cgttgaatgg gttagcatgg
    27601 tcagcagcaa gtaattgcac ttcaagcaag ttgaagatgt ggtgcacacc tgacacgacg
    27661 atcaattggt gaaccccacc aatcaagaaa ccaccaagac caaatggcat gctaagaatc
    27721 gcttttgtag caataaggat gtagttttca acaacgtgga aaactggtcc aatgacaaag
    27781 aatccaagga tagacatgac caaaagtgtc acgaatggtg ttaccaagag gtcaatgaca
    27841 tctggaacaa ccttgcggac agctttttca aatttagctc cgacaacccc gatgatgaag
    27901 gctggaagaa cggaaccttg caaaccaaca acagggatga aaccaaagaa gttcatcgct
    27961 gttacttcac caccttgagc aactgcccaa gcgtttggaa gtgagccaga gacaagcatc
    28021 ataccaagaa cgataccaac ggcaggattt ccaccaaata cacggaaggt tgaccacaca
    28081 accaaacctg gcaagatgat gaaggctgta tctgtcaaga tttgtgtgta agttgcaaag
    28141 tcacctggaa gtggcatttc aagagcgttg aaaagaccac gcacacccat gaagagacct
    28201 gtcgctacga taactgggat gattggaacg aaaacatcac cgaaagtacg gatagcacgt
    28261 tggaaccagt tcccttgttt agcaacttct gctttcatgt catccttaga tgatgttggt
    28321 aagccaagta caacaacttc atcgtacatt ttgttaactg tacctgtacc aaagataatt
    28381 tggtattgcc ctgagttaaa gaaaacacct tgaacttttt ccaagttctc aatcacttct
    28441 ttattgattt tctcttcatc tttgaccatg acacgtagac gagtcgcaca gtgggcaaca
    28501 ctattgacat tttcacgtcc gcccaaggca tcgatgactt tttttgcaat ttcctgattg
    28561 ttcatttgca aaaatctcct tatataacat tttgttcttg tttgaaagcg attttattcg
    28621 cccttacgac tattatttta tcatgtttta aaaatatgtc aagcgttttg cataaaaaat
    28681 attctatcca cttaatgggc aagctttaga ttgattgttt ttgactgttt catcaggaat
    28741 tccttgaaaa ataagtttga aaccttggtt aaaaagtctt ttatttcatt gtctttcaca
    28801 ttttcgtaaa aatttaattg atttcctgta tttattttat gataatcgtt tgacagtttt
    28861 ttctcttttt taacataaaa ggcttgcatt ttttatcgaa aacagttact attaaaagtg
    28921 ataagatttt tggaggaatg aatggaatgg acaactgagc gtcgttacag actttatcaa
    28981 gattggacgc aagaagaaat tcaacatata aaggaaaata tggcacaatc tccatggcat
    29041 actcattacc atgttgagcc aaaaacagga cttctcaacg acccaaatgg cttttcttac
    29101 tttgatggca agtggatcct cttttaccag aattttcctt ttggtgcagc ccacggttta
    29161 aaatcttggg cacagctaga aagtgatgat ttgattcact ttaaagaaac tggaatcaaa
    29221 gttttaccag atactccatt agatagccac ggtgcctact ctggttctgc catgcaattt
    29281 ggcgataact tattcctatt ttatacagga aatgttcgcg ataaaaactg gatccgtcac
    29341 ccataccaga tcggtgcttt gatggacaag gagggtaaga ttacaaagat tgacaagatc
    29401 ttgattgacc agccagcaga ctctactgac cacttccgcg atccacaaat ttttaacttt
    29461 cagggtcaat attatgccat tgtcggcgga caagacttgg agaaaaaagg tttcgttcgt
    29521 ctctacaagg ctgtcaataa cgactacaca aactggcaag cagttggcga ccttgacttt
    29581 gctaacgacc gtactgccta catgatggaa tgtcctaatc tggtctttgt agaggaacaa
    29641 cctgtccttc tctactgtcc acaaggattg gataagaaag ttctagacta cgataatatc
    29701 tttccaaata tgtataagat tggggcttcc tttgacccta aaaatgccaa aatggtagat
    29761 gtgtctcaac ttcaaaacat ggattacggt ttcgaagcct atgcaactca agccttcaac
    29821 gctcctgatg ggcgtgctct agcagttagc tggcttggtt tgccagatgt ttcttaccca
    29881 tctgaccgtt ttgaccacca aggaaccttc tctttggtca aggaactcac tatcaaagac
    29941 gacaagctct accagtatcc agtcgctgct attaaggacc ttcgtgcttc tgaagaagcc
    30001 ttctcaaacc gttcccaaac caagaacact tacgaacttg aactcaactt ggaagctaat
    30061 agccagagcg agattgtctt acttgctgat aaagaaggta agggactttc aatcaacttt
    30121 gaccttgtaa acggtcaagt gacagtggat cgtagccatg ctggagaaca gtatgcccaa
    30181 gaatttggga caactcgttc ttgccctatc gagaatcagg ctactactgc tacaatcttc
    30241 atcgataact ctgtctttga aattttcatc aataaaggag aaaaagtatt ttctggtcgt
    30301 gtcttcccac atgcggacca aaatggtatc ctgatcaaat ctggaaaccc aactggaact
    30361 tactatgaat tagattatgg tcgcaaaact aactgatgtc gccaaacttg caggcgtcag
    30421 tcctactacc gtttctcggg ttatcaataa aaaagggtat ctatctgaga aaaccatcca
    30481 aaaagtcaat gaagccatgc gagaattggg ctataaaccc aacaacctgg ctcgtagtct
    30541 gcaaggaaaa tcagctaagt taatcggctt gattttccct aatatttcca atgttttcta
    30601 tgcagaattg attgataaat tggaacacca actcttcaaa aatggttaca agaccatcat
    30661 ctgcaacagt gaacatgatt ctgagaagga acgcgaatac atcgaaatgt tggaagccaa
    30721 tcaggtggac ggcatcattt ctggtagtca caacctagga atcgaagact acaatcgtgt
    30781 gacagcgccg attatttcct ttgaccgaaa cctatcgcca gacatccctg tcgtctcctc
    30841 tgacaactat gctggtgggg ttcttgctgc ccaaaccttg gtcaagacag gtgcccagtc
    30901 tatcatcatg attacaggga atgacaattc caattcgcca accggactac gccacgctgg
    30961 ttttgcatcc gtactcccaa aagctcctat tatcaatgtt tccagtgact tttctcccgt
    31021 cagaaaagaa atggaaatca agaatatctt gacccgggaa aaaccagatg ccatttttgc
    31081 ttcggatgat ttgacagcta ttctggtcat taaaatcgct caagaattgg gcatttctgt
    31141 cccaaaagag ctcaaggtca tcggctatga tgggacctac tttatcgaaa attactaccc
    31201 tcaattggct actatcaagc aacctttgga agagattgct tgtctcacta ttgatcttct
    31261 cttgcaaaag attgaaggca aggaagttgc cacaactggt tacttcttac cagttacact
    31321 attaccagga aaaagtattt aaacacaaga aaactcagac cgattcgtct gagtttttta
    31381 tgatcttaaa ttttcgagat agcgctgggc tgtctctagg ttaaaggttt tatctgagat
    31441 gaggcgctct actaggggag caacttcaga ttcactagcc ccagctagga gagctaggga
    31501 tttggcctgt agtttcatgt ggccttgctg gatgcccgta cttaccaaag ctttgagggc
    31561 tgcaaaattt tgggcaagac cgatggacac gataatctgg gctaattctc tggcagaagg
    31621 atttcctagt agatcatgac tgaaagctac acgtgggttg aggccgatag agccaccctt
    31681 agtcgctaca ggcatgggca gggtcatctc accgaccaat tcttctcttt caaggtccag
    31741 cgtccagcaa ctaagacctt gatagcgtcc atctcgactg gcaaaggcat gggccccagc
    31801 ttcgatggca cgccagtcat taccagtggc aatcaaaatc gcatcaatac cattaaaaat
    31861 tcctttatta tgagtagcag ctcggtaagg atcagcctgc gcaaactgac tagccaacgc
    31921 aattttctcc gcaatctctc gtccttgatc cttttggcgg ctcaagtagc gaaaggccat
    31981 gcgacagctt gcagtcacca gagaatcggt cgcgtagttg gacaggattc ctatgagact
    32041 ctgtccctga ctgagttctt ctaagactgg tttcaaggct tccagcatgg tgttgagcat
    32101 attggcaccc atggcttcct gggtatcgac atgaatataa acaacgagaa agtctggttc
    32161 gccttttatc tgctcgacat gcagatcacg cgccccacct ccacgtttaa cgatagaagg
    32221 ataggcttga ttggcaaact ccaagagctc cgctttcttg ctggcaatct tctcttgcgc
    32281 tagtttagga ttagcaactt gataaagggc tacctgccca atcatctggc gctgatggac
    32341 ttgtgcagta aaaccacctg cacgcttgat gattttgctg gcatagctgg ccgccgcaac
    32401 cacagagggt tcttctgtca catagggaac ggtgtattcc tgaccgttga caagtacctc
    32461 cggaaccagc gaataaggca gagaaaaagt tcccactaca ttctcactca gctggtctgc
    32521 cacagtcaca ctcatctgtt catccttctc cagactagct tgtctctcag gactaaggag
    32581 cgcctgagct tttagcagct cgaggcgctc ttggtatgat tttttagaaa atccattcca
    32641 acttatcttc attatttttc aaccttgcta taacggcgtt ggtggtcgag aatttcaacc
    32701 aaggcaaaat cttgattttc atagccagca aactgggcag agtttgtttc atccaagttt
    32761 acttcctcaa aaaagacctt ttcatagtct gcaacggata aggcagttcg ttggttgagc
    32821 ttgttcaagc ggtctttatc caaataagct tcatatcctt caaccaattc accactgaag
    32881 aactcagcca cagctccact tccgtaacta taaagggcga ttttatcccc agctttcaag
    32941 ctatctgcat tttccaagag agacaaaagt ccaaggaaaa gtgaacctgt gtagatattc
    33001 cccacctttt gactgtagag aatagactgg tcaaaatgct tttgtaagag gtcttttttc
    33061 tcttgaggca ggctcttatc catgattttt ttcaagcctt ttagcgctaa tttaggataa
    33121 ggcaagtgga aacaaacagc cgcaaaatca tccagagtaa gctggtagcg tttttgatat
    33181 tcaagccaag tcgttttcaa actatccaag tattgttggg tagaatagac accatttaca
    33241 taaggagttg tcgagtaatt tggtcgccag aaatccatga tgtcacgggt ctgagctaca
    33301 ttgtcattat taaaggccat catgcgtgga ttttgtgtaa tcaacatagc tacacttcca
    33361 gcaccttgag ttggttctcc cggagtttca ataccatatt tggcaatatc actggcaatg
    33421 accaagacct tggactccgg agaattttcc acatgcaatt tggcataatg gagggcagct
    33481 gtcgccccat agcaggcttc tttaatctcg aaactacgag caaagggctg gatgcccagc
    33541 aagccatgca caaagacggc cgcagcctta ctctggtcaa ttcctgactc ggtagccaca
    33601 atgaccatgt caacttcttg tctttcttgc tcagttaaaa tagagtcact agcactggcc
    33661 gccaaggtca cgatgtcctc agttaggggc gcaatactca attccttgag taagagtcct
    33721 ttacttaatt tttcagggtc aattcccctc gcttctgcta agtcttgtaa tttcaagaca
    33781 tattgactgg tcgcaaaacc aatcttatca ataccgattg tcatatttac ctctgtttta
    33841 tcattcatgt aaaaaatcgt tctatactat tttatcacaa atggcagtaa aagagagaaa
    33901 aaagacttga ttcaccaaat caagcctctt attggtcatc attttaaaga atgattagtt
    33961 gctagagagt tcaccgatat aagtagcttt ataagctcta ttcacagtta tcagctcctg
    34021 gaggatcaaa tttcctgagt aaatccttcc catctcatct acaaattttt gataaaactg
    34081 actggtcgga atttctctga catccttatc aaatgtctta tcaagtgttt tactaacctt
    34141 ctcagcaatc aattgatgct cttgccatcc actttgaaac tctgagcccg aactagaaac
    34201 catgactggg ataaacaaca aggtcagtag atttacagac aataaggaaa gtagtagact
    34261 tcctgcaaaa ctagaatcct agttcatgat tgataatgcc agcaatcaaa ttcattcgta
    34321 atccgaagcg tttacgatga tttcgatagg ttgttgaaaa cattttaaac gtttttactt
    34381 tggcaaagat gttctcaacc ttgcttctct ccttagatag cgcatggtta taggctttat
    34441 cttcaactgt tagcggcttg agtttgctgg atttacgtgg agtttgtgct tgaggatata
    34501 tcttcatgag cccttgataa ccactgtcag ccaagatttt accagcttgt ccgatatttc
    34561 tgcgactcat tttgaacaac ttcatatcat gacaatagtt cacagtgata tccaaagaaa
    34621 caattctccc ttgacttgtg acaatcgctt gagccttcat agcgtgaaat ttctttttac
    34681 cagaatcatt cgctaattgt ttttttagga caattgattt gtacttccgt cgcatcaatc
    34741 attaccgtgt cttcagaact gagaggagtt cttgaaatcg taacaccacc tttgaacaag
    34801 agttacttca acccattggc tccgacggag taagttgctt tcgtggatac caaaatcagc
    34861 cgcaatttct tcataagtgc ggtattctcg cacatataga aagcgttgtt aatttattta
    34921 tctcattttt cagaaaattc ttttatttct gtaaagtcta cgatactcga tgtgttttta
    34981 tataatgata cagtctgaga atcactgttc cgctagccat tccaatagag attaccaaag
    35041 ccaacatgac aaccaaggtc gcacttgcca gtgctttatt atagtcccct gtcacaaaaa
    35101 aggcagttgt tcggtaggag agataacctg gaaccagcgg tgccaaaatg gccaagataa
    35161 agaccacagc aggtgtctta taaagaatac ttaaaatctg gctgacacaa gaaccaataa
    35221 tggctgcaat gaaggtagct acaatgacat tggtcggttc cttgagcaag agatagatta
    35281 gccagacagt catgcccaaa atccctccag gtaagagcat agaccgttgc acattgagta
    35341 cgattaaaaa agtgataatg gcaagaaaac ttgctactgc ttgtaataaa aaggttgtta
    35401 gtgtcatatt agttcatcaa taccaaggcg acagaagttc ctgcccctaa agcgagggta
    35461 atgagcaggg attcaaacat cttactcata ccagagttta tgtggttggt cataatatca
    35521 cggaccgcat tggtcaaggc aatacctggt acaaacggca tgaccgcacc agctataatc
    35581 aaatctgccg ttgaaggaaa acctgtgtag cgagcccaaa actgggcaat tatcccaaag
    35641 acaaaggctc cagcaaaggc tgtcacaaag ggaattcgga taaatttttc cacatagagg
    35701 gaaaaggcaa aaccaaataa ggtcgccact cctgccccaa gtgcgtcgta gatatttccg
    35761 ctaaacataa ctgaaaagaa aggagcacta aaggtcgcag ccagagttac ctgcaactta
    35821 gtatagggaa ggggttgagc ttgcaaggcc gtcaattgct taaaggctgt ttctaagtca
    35881 atctgccccc caactagctg acgagaaatc tggttcacat cgcagacttt ttcgatgtta
    35941 taagaagagg aggtcacgcg cttcatgcgc gaaatattgg tattttcaat agagaaaaag
    36001 atagcggcag gcatggcaag gacattgcaa tccacaatcc cctgcgaatg cgcgattcga
    36061 atcatggtat cttctacacg atggatttct gagccacttt taaggagaat agtccccgct
    36121 agcataatca catcaatgac ggcatttaat tcttttgatt cttccatgct ttcctccttt
    36181 tatcaactcc ctctattcta tcacaaatct ggactcaaaa aaaatctttg ccatgaaatc
    36241 atgacaaaga ttgattactc attttgatta tccatctgct tttaaggagt agctgaagtt
    36301 gttttaggtt tgtagattga aatcttgact ctagtcttat ttaggtctac cttttcacct
    36361 gctctaggac tttgttcaac aaccatgcct tctacactac ctgcaggcgc tgtcgtcact
    36421 tctacaactt ctatattagc ttccttaatc ccaacaattt gaatcaaatt gttcttagta
    36481 aactccaagc tggaaccaat gtaactcggc atggcaacac ttgtaacttt tttagctact
    36541 gtcaagacaa tttgagtagg tttactcaca tcataagtcg ttccggcacc tggactttgt
    36601 ttcataatcg ttcctggttc gctttcgctg gactcttctt cctctatctt aatcaaattc
    36661 tcaggaacct tcttctgctt gagttctgag attacttctg tagagttccg tccaatatag
    36721 ttccctaatt gaatcgtcgt agctttttta gctactgtca aaacaatttg agttgccttg
    36781 ctcaagtcat aggtcgtacc ttctggtaga ctttgcttca ggaccgttcc agcctcactc
    36841 tcattcgact cttcttcctc aattttaatc aaattatctg gaactttttt ctcttttaat
    36901 tccgcgataa catcagaaga tttccggccg acataattac taatttggaa ggattgtttg
    36961 cctgatgaga caaccagatt gattttcgtt ccttcttttc gaccagttcc agcgccagga
    37021 tctgtacgga taatccgccc ttcttccacc ttttcactag cctctgtctt ctcctcacca
    37081 atctcaaaat tggctttttt gagcgttgcc ttggcctctg caactgtctg acctgccaca
    37141 tctggaatgg caatggttgc aggagttctg gatagtatcc aaataagaga agctgccacc
    37201 aatacaaggc tggccaacaa aatcaggtaa cgcatcttaa atctatgttt tttcggtgct
    37261 tgtggttggt aagtttcctc tgtcacagcc tggcttgggt ttttgattga tttgtgttct
    37321 gtctgcgctt gaaccttagg aatagatgtc aaggtactct gagaaacctt cggcaaggtc
    37381 ttggtatctg ccttgctcgt ttcatcaaag attaacttac tttcatttct acgattgtag
    37441 gacaagctac tagacaagtc cacatacatc tctgaaaccg agcggtagcg attggtcaac
    37501 tttttagcag ttgccttgat aataacattt tctaaagcct gaggtacaga tggattttct
    37561 gcaataacgg acggcagggg tttctggaaa tgctggaggg caatggtcac cgcgctatcc
    37621 ccgtcataag ggatatggcc tgtcaacatc tcatagaaaa taatccccat ggcatagata
    37681 tcactctgca cagtcgcctt cgaaccacgc gcctgctctg gtgacaagta atgaactgag
    37741 cccaacatcg agttagtctg ggtcagactt gtctctgcaa aggctacagc aatcccaaag
    37801 tctgtgacct tggccgtccc atctggtgtc aaaaggatat tttgaggttt caagtccctg
    37861 tgaacaattc ctcgagtatg ggccaagcgc atagccaaga gaatttgtcc catgatacgg
    37921 actgcttctt cattagaaag aggataatgt tccttgatat agcgtttgag gtctagtcca
    37981 gcaacatact ccattgcaag atactgttga ccgtcttcct caccaatatc tgttatccga
    38041 acgatatgag gatggtctag atctgccata gctctcgctt cacgctgaaa acgagctaca
    38101 gctatcgggt ccgtctggta gttggtcctc agaaccttca ctgccacttc ttccccatct
    38161 aagattaagt ctttggctag gtagacatcc gccatgcctc ctcgaccaat ctgtttgaca
    38221 atccgatagc gtccggcaaa aatcttgccg atttggatca ttcttcatcc tcctcgttca
    38281 tagaaacaag ggcaaccgta atgttgtcta aacctcctgc attgttagca aaacgaacaa
    38341 gtgtctccgt tttatctgct aaaggaatat cactggttac aatatcacga atctcactgc
    38401 ctgaaatcat gttggtcaag ccgtcactat tgagcaagag atagtcacct gactcaagga
    38461 taactgtccc aaaatcaggc tgaatttcat ctttttgccc aatagactgg gtgataatat
    38521 ttttttgcgg atgagcttct gcctcttctg gtgtcaattg accagccttg agcaattcat
    38581 taaccaagga atgatcgctc gtcaactgat ggtattcttc tccacgaatc aagccgatac
    38641 gcgaatcacc aatatgagca tagatagcct gattatcaat aatagcaagg acttccaaag
    38701 tagttcccat gcctctgtaa gcttcatcct gaccaagctg gtgaatcttt tgattttcaa
    38761 tttctaggta atgggcgaac cattcacgca cttcattgac tgtatcgatc tgggtatcaa
    38821 cccaagctac acccaggtct gtgaccgcca tttcactagc gatattccct gcacgatgac
    38881 ctcccatccc atcagctaaa ataatcatgg tacgtccagc tctattgaca tagtggttga
    38941 catagtcttg gttatttgtt cgtttctgac caacatctgt taataatgaa atttccatgt
    39001 gtcagttcct tcctaatccg atatcttgcg aaattgactg atgaagaatc catcacttcc
    39061 atacaattca ggtgtaatga ggatacagcc gtctttcatg atatccttac attcatgttc
    39121 tagttttacc tgctcgaact cgggatgact ctctaaaaag gccttaacaa cttgaaaatt
    39181 ctcctctgag acgatagtgc aggtgctata agttattata ccacctttgc ctagtatttg
    39241 acaaacacta cctaatattt ctaactgaat ttcctgcaag gacgcgaaat ctgccgtttc
    39301 tttattgtat ttgatatctg gttttcggcg caaaagaccg attcctgaac aaggagcatc
    39361 caccaaaatc ttatcaaagg aatcctggtc aaaaaactca tgcacctttc tggcatccaa
    39421 tttttgagtt tgaacccgat cttcaactcc cagacgttgg gcattttctt gaattaaatc
    39481 caacttgtgg tcgtacaagt ccagagcagt aacctgacct gtcgtaagat aagaggctat
    39541 atgggctgtt ttcccacctg gagccgcaca ggcatcaagc actcgctcat caccttgtaa
    39601 atcaagcgtc ggagcaacca actgactgga ctcgtcttgg atggtaatgg ctccatccgc
    39661 aaacaaatta tgccctgcaa aatgcccctg ctccttaacc agaccagtgg ttgctaaaag
    39721 ggaattattc gcctccaaca aggcttggat ttcctctttt cgacttaggt ctgttacacg
    39781 aatactggct ttgtttcgca ctaacaggct ttcaaagatg gcttttgctc tctcctctcc
    39841 gtattcttcc ttgagtttgg caactagcca aactgggaga gaataggcaa tggagtcacg
    39901 cttgtttttt cgcttgatgc tagcaatatc tggccagcct tcacgcaaga tacggcgaag
    39961 gacagcgttg accaattttt cactgccttt tttacggagt ttggccaatt ccactgcttc
    40021 attgaccaca gcatgatctg gaatcttgtc caaatagcgg agttggtagg cactcatgag
    40081 aagaaggaca tagagccagc tgtctaactg gtctctgtct tcgataaagt gggataggta
    40141 ccattccaga gtcagtttac gggctaccgt tccatagacc agctcggtca ctaagccctt
    40201 gtctgctgcc aaaagttgac ttccctttag atgcttattt aaggcgatat ttgagtatgc
    40261 ttggttcaca aaaacatcct ctagcactgc tagagctaaa cttctagccg tttctacttt
    40321 agtcaccaaa tcgttctcct acagtcaatg tacgtccaac tccgttgagg aaggaagcaa
    40381 tgtccatctt aggcttacca gctggctgca cttgtttgag ggatagagcc ccttcagccg
    40441 ttgcgacaat caattctttc ttgccgatag agagaatctc acctggattt ccctgacctt
    40501 ctactggtag ggcttcataa atcttaaagc ggtcgccctt aaggaaagta tgggcaacag
    40561 gccaggggtt cattccacga atttggttaa agagttgacg attggttttg ttccagtcca
    40621 gtttttcttc ctctggcttt atatttggag agaaggtaac ctgactcgta tcctgcggtt
    40681 caggtttgat atcaccagca atataggcag gcagagtgtc caaaagcaaa tcacgaccaa
    40741 ctagcgccaa tttttcaaac aaggcgccaa cattgtcctc atctgtgatc ggaatgctgc
    40801 gacgagaaat catatctcct gcatccattt ccttaaccat ttccatgatg gtcacaccag
    40861 cttcctcatc cccttgaatc aaggcataat ggataggcgc accaccacgg tgtctaggaa
    40921 ggagggaggc atgaacgttg acagcaaagt ccatgctatc aaggagtttg cttgggagaa
    40981 actgcccaaa agcagcagtc acaattccat ctgctcctag cttcataaga tcttccatct
    41041 ctggacttcc agataatttt tcaggttggt agatagatag tcctgcttcc ttggcagcct
    41101 gcttgactgg ggtttcttgg ataacttttt tacgaccaac agcacggtct ggctgggtca
    41161 caacggctag aatttcgtaa cggtcatctg tcaaaagtcc ttttaagact gttgctgaaa
    41221 agtcgggggt ccccataaag attagttttg tcatatcttc tccttcttat aaaaattgct
    41281 gcggctcatg gtcaatgctg agacggagct cactattttc ccgttcttga gtcaaggcca
    41341 ggacctggtt gagggtcggc cccagctcat cttctaaacg gtatttaatt aaaatctggt
    41401 aatgatagag gttgtgggta cgggcaatcg gttttggcgt tggccccaga atggaactgg
    41461 tctctgacaa gcttgaccgc aaaatattca tgacttcata ggcacgtttg accacctctt
    41521 cttctttctt gtgagaaagg gtaataccaa tcgtgaaata gtaaggtgga tagccgagtt
    41581 gtcgtctgat tcccatttca taggcataaa agccttcgta atcttgatcc ttggcaaatc
    41641 gaatagcata gtgctgcgga ttgtaggact gtatcaagac ttgacctgcc ttttcagccc
    41701 gacctgctcg acctgccacc tgagtcaaga gctggaaggt tctctcagaa gaacggaaat
    41761 caggcagatt caaggccgta tccgcattta gaactccgac tagggtaaca ttgggaaaat
    41821 ccaacccctt tgcaatcatc tgagtaccaa gtaaaatatc cgcttcccct cgcccaaact
    41881 ggtcaagcaa ggcttggtga ctgcctttct ttcgagtcgt atccacatcc atcctcagaa
    41941 tgcgagcttg gggaaagagt tctgctagct catcataagc cttctgagtt cccgtcccat
    42001 agtaacgaat actgcggctc ttacagttag gacagacctg aggaatatcc ttcgagaaac
    42061 cacaataatg gcagttcata gtcttggtat ccatatgcaa ggtcagagaa atatcgcagt
    42121 tgggacaagt atccaccgtc ccacactccc gacacatgac aaagctagaa taaccacggc
    42181 gattgagcat gagaaccacc tgctcttttt taaccagacg gtcttggata gcctctagca
    42241 aaggaggcgt aaagtttgac gtctcatttt gtccgatata gtctcgaaag tcaatcactt
    42301 gaacctcagg gattgtagcc aaaggattgg cacgttgggt tagacgtaag tgttgataga
    42361 cgcctttgcc agcacgtgcc cggctctcta agctcggcgt tgcagatcca agtaccagag
    42421 ttgcttgatt atactgagcc cgtaaaatag ctacctctct ggcatggtaa cggggattgc
    42481 tgtcctgctt ataagccgct tcatgctctt catcaataat catgacaccc agatttttca
    42541 gaggagcaaa gatagcagat ctggcaccaa caacaacttg ggcatcgcca cgctccacct
    42601 tgcgccattc atcatacttt tcaccattgg ataatcctga gtgaagaatg gctaccttgt
    42661 ccccaaaacg tgctataaaa cgctcggtca tctgaggagt caaggaaatc tcaggtacca
    42721 gcaaaatagc tgtcttgccc ttatccaggg caccttggat aatctgcaag taaacctcgg
    42781 tcttcccact tcctgtaatc ccttgaagta gaaagggagg ttgagaactg ccaatagaac
    42841 tcacaaccgc atcacgcgcc tgtctttgtt ctggatttaa ctccaaaggt ctacttgctt
    42901 caattccttc aaaataagca gccgagcgtt gaacttcctt ttggactatg gtaacagcac
    42961 cttgatccac aaagaagttg acttgctctc gcgagtagga ctctaacaag ctagccaagg
    43021 aagcgctctc tggatgagac agcagataat ctctcagttc caactttttc ttggcacgtg
    43081 tagaaatctc aacaccttct aattgagcat ggtcaacctc ataccaagac tgggtcttga
    43141 ccttcttttg atcgactgcc tgatattcca gaccaagcag gccttttcta gtcaaacgca
    43201 tcatttcagc ttgcttggca aggtctagtg aagaaaaggc tagcgaatct tctgaaccaa
    43261 acaggcgcac tcgttcttcc tgactcaagc cttccagagg atagagaatc ttgtcatagc
    43321 tagaattcag aaatcctgga agcatggcct tgaggataga aattttgtag gagaagacag
    43381 atttgcgtaa ctcctcagcc agccagagtt gttctggcgt gagaacagga gaaaaatcca
    43441 gcacctctgc aatatctttt aaatcttgct ccatctcttc tccatctgat tgggactcca
    43501 aaccaagaac aatcccttga atcaggcgat tacccttacc aaaaggcaca tgaacccgca
    43561 tcccaacttc cagcattccc tcaaattcct ccggaatcct gtaactatag ggctggtccg
    43621 tctgcatcaa gggcacatct acgataatct tagctagggc catcttctca cctcctcctt
    43681 gtcagtacat tcttgcaata gaaaaaataa gattgagtcc ccccaacctt aaattttttc
    43741 accatcttct ttttctttag caatttgctc tttgattttc ttttcttctt cttctttgcg
    43801 gcgtttttct tcttcgatac ggcgacgcac tgcttcacgt tttccttctg gatctgggtg
    43861 aattgtaacg tttcctgatt cgatttcttc taaagcgcga agagttgatt tttcagactt
    43921 gaaaccttga gttgctgggg cacctgcttc caattcgtgg gcacgttttg cttccaagat
    43981 tacgagtgaa tattttgaag gaaccttgtc gagcaaggta tcaatagagg gttttaacat
    44041 catttgcttg tacctatttt ctaaatttta tcgggtagtt ggagattttg gtaacatctc
    44101 ctgatagtga ccaatgacac gatccacaca gaagtgttct gcttcaatca cacatttgac
    44161 acgttcagca gctagggaca cctgatcgtt gacaatggca taatcatact cacgcatgag
    44221 ggcaatttct tccttggcct tttcgattcg ttgggcaatc acttctgcac tatctgttcc
    44281 acgacctacc aagcgatctt gcaattcatc caaatctggt ggtgtcagga agataaagac
    44341 agcatctgga acctttttct tgacctgaag agcaccctga acttcaattt caaggaaaac
    44401 atcgattccc ttgtccaagg tttcattgac ataggtcaga ggagttccat agtagttacc
    44461 gacatattct gcgtattcca acatctgtcc ttgacgaatc agctcttcaa attcttcacg
    44521 agtacggaag aaatagtcaa caccgtccac ttctccagga cgttgtgcgc gtgtcgtcat
    44581 cgatacagag tattgaaatt ggttttcaga actctcaaaa atctctcttc taaccgttcc
    44641 ttttccaacc cctgaaggac cagaaaaaac gattagtaag cctcggtctg ccattgtgtc
    44701 tccttttagt caatctgtga aataacattt ctctagaata atggcaaaaa gccagattat
    44761 cctttacagt ctttctatct agtgtaacaa aaaagcagta atttttcaac tgctctttct
    44821 tatttattta gcataatcta ctgcacgaag ctcgcgaatc acggttacct tgatatttcc
    44881 tggataatcg agattgtttt caattttctt acgaactttg tgagccaaga ttgtgacttt
    44941 gtcgtccttg atttttcctg gattgaccat gatacgaatt tcacgtcctg cttgaagggc
    45001 aaagctagtt tgcactcctt caaagccgtt agcaatttct tccaaatcat ggagacgctt
    45061 gatgtagctt tcaagagact cactacgagc acctggacgg gctgcgctca aggcatctgc
    45121 tgcagcgacg ataactgcta tcacgctctc agcttcaaca tctccgtggt gactagcaat
    45181 cgtattcacc acaactgggg gttccttgta cttacgggcc aattccatac cgatttcaac
    45241 gtggctacct tcaacctcat ggtcaatggc tttcccgata tcgtgaagga atccagcacg
    45301 acgggcaaga gccgcatttt caccaagttc gctcgccatg ataccagcca acttagcaac
    45361 ctcaatcgaa tggcgcaaaa cattttgtcc atatgaagta cggaactgca aacgtcccat
    45421 aatcttcatc aagtctggat gaaggtttgg cgcaccaatt tcataggcag cagcctcacc
    45481 gtattcacga atcttattgt caatctcttg acggtttttc tcaaccaact cttcgatacg
    45541 agctggatgt atacgaccat ctttgagcaa catttccata gtcatacggg caatctcacg
    45601 acgaatcgga tcaaatcctg acaaggtcac cacttctggt gtatcgtcga taatcacatc
    45661 gacccctgtc aaactttcaa aggtacgaat gttacgacct tcacgaccaa taatgcgtcc
    45721 cttcatagta tcgtctggca gatgaactgt tgagtttgtt gactccgcta catattcacc
    45781 agcgatacgt tgcatagctt gaaccaagat gtccttggcc attttgtcag aacgttcctt
    45841 gacctcttgc tcagcttcgc gaatgcgact ggcaatctcc ctggtcaagt tttcctctgt
    45901 ctgagccaag ataatatctc gtgcttctgc ctgagacaga gcaccaatac gctctagttc
    45961 tgcttctttt tgtctttcga cttcctctaa ttgctcttca cgcgcatcaa ggtttttcgc
    46021 tctatcagaa atactttgtt ctttttgttc aagtgtttgt tctttactcg tcaaattgtc
    46081 gtccttacgg tcaaggctag tagctctctc tgtcaaacga ctttcgattt gtttgagttc
    46141 ttgacgttct gatttgaatt cagcgtccac ttcttcacgg tattttctgg cttcttcttt
    46201 ggcctccaat agtgcttctt ttttaagaga cttgctttca cgtttggctt cattaacaag
    46261 taaatccgct tcacgctcag cttgtccacg taaattagtt gcttcttgtt cagcatttaa
    46321 aagcatcaac tccgcagctt cctgagatga tttcatctta gctgagatgc tgacatatcc
    46381 aatgactaaa ccaatgatga cggcaaaaac agcaatcgca agcgacatga tctccatgtt
    46441 tttacctcat tttattgtta ttccgaatga catacattct tttacattct accataaaaa
    46501 agtgattttc acaaacctaa aatagaatat gttttgggga atttggaata tatttgccaa
    46561 aataaacttg ttttttagaa atagtagttt agtagagact tgagaaaaag cctacctttc
    46621 aatagactta gtaatgatct ttaaaggaca agaaagccac gctatctcca tccatcatat
    46681 aaatcaagcg attttctgca tcaatacgac gtgagcaagc tccttggtag tcatacttga
    46741 gtggttctgg tttacctatt cctgtaaagg gatcacgttg aatatccttg attagtttat
    46801 tgattctttt taacgttttc ttatcctgat tttgccagta gcaataatct gcccaggcat
    46861 cttctgtaaa cttgagcagc atttcttact cctcaataac atggacctga gtacttccag
    46921 cacgaacttg agccattcct cgcaaaacct tatcagaaag ttccttattt tgagcaattc
    46981 tcagggtttc ttggatacta tcccactcac tctttgaaag gactacaatg tcctcgtctg
    47041 gatttttatt gaccaccgtc aaaggctcaa attcatcatt taccttcttc atgtagtcct
    47101 ttaaatgatt tcggaatgtt gagtaaagga ctgcttccat aaccatacct cgttttagct
    47161 cttttccact attatacaca aaaagaaaga aattgtcagg aacttgtaca agattttctt
    47221 ttctatctat ttatactcaa tgaaaatcaa agagcaaact aggaaactag ccgcaggctg
    47281 tacttgagta cggcaaggcg acgttgacgc gatttgaatt tgattttcga agagtattat
    47341 tcgtaaaaaa tctcaaaaag cctacctttc ggtagactta gtttgtttct attctaatcg
    47401 gcactcttcc aaaattttgc tctgctatac ttggcttccc tagttggtaa atctggtctg
    47461 ctttttgggt catagcatcc caaggttctt tgccaattcg gctgactaga ctgacctgtc
    47521 ctttcagaga cttgagatgt tgcctgcctt tttcagtaaa cccaaggaca tgaatggctt
    47581 ctggcaagac attttctcta gcctgcacca agatatagga caagaggcgt ctgacacgcg
    47641 ccttggtgta acgtttggta gtcactaact cgaccaattc ttccacagac tgggctgttt
    47701 taatagcttc tttaatgcgc acagccattt cttgattgac ctgatagatg gtggttaggt
    47761 cgggatttga caagatttga tagcggagca agggaaaata gtcttcccag atcaccttac
    47821 tggcctgctc aaatagggca acagaaggca taaaacgttc taagaaatct tggtccttct
    47881 gatgttgacg gagggctgtc gccgaggcaa agtccacatc tttattcaca gaatggtaac
    47941 ctgccccctg acgctgaatc ggatacagct tgatattccg tcctgcaacc gccttggcat
    48001 aggccagagc aagaacatga ttgggggtat tacctgaaaa atcaagacca gcaaattcct
    48061 tccacatagc ttgggttttc tggggatagg aaagggaatc aggcagattt ttcacaaatt
    48121 tctccatctc agcacctttc tctgtgtata agtcagcgat tttctggtaa tccagaactt
    48181 cctctgtccc aaaaactaga ctatcaatgc ccaagcgatc caagatatcc acagctcctt
    48241 gaccaaagaa atcagctgcc tgaacactga ctaaaaaggg caattccact accaagtctg
    48301 cgccattttc cagcgccatc tgggtccttg tccacttatc cacaatagca ggctctccac
    48361 gctgcatgaa attccctgac atggcaacaa ttttcagtcc ctctgcctga tccagcaggt
    48421 atttatggcc attatgaaaa ggattgaact ccgcgataat acctgtgatg gtcatgctaa
    48481 tctcctactt ctgcgccaca aaaaaccaac gggtgctagt ttctgttggc tccttatcct
    48541 caaagtctgc atagagtttg aaggacttaa atccagcctg ttccagcaag atatcatagg
    48601 tcaagatctc ataggtcctc tcctcatgta cttcatcgtg gcgactaaag gaaccgtcag
    48661 cctccttgat aaagaaagtc agctcatgca cgatggagtg aggaacttcg tcctcatatg
    48721 tatcccaaag catggcaaaa tcttccgcat tttcatggta ggaatagcca gggaatactt
    48781 catctgtctg gtaggtcgag tgcacgtcaa agatgaaaac tccctcttca ttcagcgcat
    48841 tgtacacgtc cttaaagacg tcccctactt ccacctcatc ctgcatatag cagatagagt
    48901 ccgaataaca cgtgacaaaa tcgtatttcc ctgccttgga caaattcagc atattacctt
    48961 ctataaaagc aatcttttgc ttggctgaag ttgctctctt ctccgcaatc ttcaacatat
    49021 ccgcactcaa gtcaagtcca gtcacatcaa aaccagcctg agagaagcgc actgattgaa
    49081 ttcctgtccc acaagccaat tccaagagtt tctttctctc cttggtctta ggcaaatgac
    49141 gcagagaaaa attcgtccat ttgtcgtata aactatcgtc catgaccgca tcatagaccg
    49201 ccgcaaaggt ttcataagtc gccataatca tgatactaag agctccatgg taaacaccac
    49261 tagcccttta cctttctatc aatttttcta aaataagcaa agaaacccag ttgactacaa
    49321 ctgagttcat cttctaagca agagcttctg aaatatctac tgaattcgcc tcatgccata
    49381 gcttctctag attataatgg gcacgcattt cttctgaaaa gatatgcacg acaacagcac
    49441 cgaggtccag taagacccag cctccagctg catcgccttc gacatggcta cctttaaagc
    49501 ctgcttgagc tactttttca cggatattag ctgcgatagc gtccaactga cggctattca
    49561 ttgagctagt gatgacaaag tagtccgtca cactagtcaa atcttgtaca tcaagtgcga
    49621 ggatatcctc cgcacgtttc tcatcagccg ctttcacgac tagttctagt aattcttttt
    49681 cgttcattta atcctctttt tagaaaatat gtttgtaatc tagaacatct gcaaagcgct
    49741 cctcccaaat gtcctcataa aattcattaa tcgtttctgc tggaatgtca tccccatcaa
    49801 aggttgtgac agcaccttct ggaacaataa gctgatagcc atattcaaag gcaaccttga
    49861 cagaggtatc cacacaatat tctgtctgca taccacataa aactaatttt tcaatcccct
    49921 gcttatccaa gtattctttt aagccagttt ctttgaaaat actgttatac ttcttctgaa
    49981 agaccttttc agcaggtttt cgatttaaaa gcgcagataa ctgccaatct tctgatgttt
    50041 gagcttcaga gttctcaata tgttgaacat agataatttc aatattcttg cttctagcct
    50101 ggttttgtaa ataagaaatt ttttccaata gactttttgt ctggaaccca gtttctacta
    50161 aaacattctg aacatcaatg attataaaag ccgtcttcat ttagtcctct ttcaaatagt
    50221 gcacaaaggc gttataggtt tcaagggttt ggggatagat ggggaatccc tgatgagcta
    50281 gatactccac ggtacgagct gtttcgtagg ccaccgcctt attgagcgat agacttgcaa
    50341 tctcacgcgc cacatctact ccaggaaagg ctcgattgtg ctcgatatag tctgctacat
    50401 agacaacctt atctaggtct gtcatctgac cagcaccgac tgtatggact tcaatggctc
    50461 gcaggatttc tgaatcatgc aaatccaaat cttcctgaat tttgtaaatt ccgaccatac
    50521 catgccagac attattaccc cagtttttga ggtcaggatc tagctggtaa cggtcaatca
    50581 aagctagaaa ttcctgatct gacaactttt tagcatagtc atgaagaaga cctgctagac
    50641 ttgctttctc aacatctaca ccaaagcgct gagctaattc catagcggca cgctccacac
    50701 ctagacaatg ggttagacgt ttctcaggta gaagctctgc cattttttcc aacaaggcct
    50761 cacgggagca gttgatatag tcttgatagg acatcagtag agcccctcct tctcgatgta
    50821 gtctagcact ggctgaggta ggagaaagtt gggtttccga ccttgggcaa tgaagtcccg
    50881 caccatgctg gacgagatat ccatgagcgg tacgtccacc cagataactg gataggaagt
    50941 ccctgccttg tagcgtggac gctgaacccc cacaaactga accatgtcaa ccagttcatc
    51001 aattcggtac cacttaggca gatagtcaac catgtcggca ccgatgataa agtaataatc
    51061 cgtatctgga ttcttctctg tcaaaatctt catggtgtcg taggtgtagg aaataccctt
    51121 gcgctccaac tcaatggttt caatgactag gccgtcaatt ccctcaattg ccaactcaag
    51181 catcttgaga cgatggtgtt cagggatggt ttccttttta tcaacgtgag gaggttggta
    51241 ttcaggcatg agaagaactt gatccagtcc caactgttgc cgtacttgat ccgcaacaat
    51301 gagatgggca ttgtgaacag ggttaaaatt cccccctaaa atcccaactt gtttgcgttt
    51361 tttctccttg atttctggct ccaactctac cttggtaaag ggagtcaata gttcgattgt
    51421 cataggctag tcccctttat ttctctataa aaacagttat ttggagtttc ggtcttagat
    51481 ttctttgact ttcttagaaa tcttgcgatt ttctttcttg ctagattgtt taaacaaaat
    51541 caagatgcgt cctatttttt ggactgtatc cacaccgatt tcttcttcca aaatttcagc
    51601 tacttcgtgg atgttttcat ctgtgttttg taagagagta accttgatta attcacgcgc
    51661 atcaagagct tgacggacgc tggttttgat ttggtcgttg agtccatttt tcccgatttg
    51721 gatgataggt ttgagggtgt gtgcctggct gttgaggaag gcacgttgtt ttgatgttaa
    51781 tgacataatt tctttaaaag agtttctttt tatacttttc tgaagtggtg acggacgtca
    51841 gcaaagtcct tcggactttc atgactaaaa tttgagccta aggtctcaaa ttttccgcag
    51901 ttggtacaat cacttgtacc aacttacacc acagcgaaaa gtattctcta tggggctcgc
    51961 cttgctcgcc caaattcaga caaccctttc ctttctgtgc ttaaataatt gcttttcgtg
    52021 tgacgacggc gacgccttct ggtgcccaga cggcgacttt tgctgtgcct gttacacgaa
    52081 tccatcctag gcctgagatg actaggtctg tcttatcttt aatggtaaag acatgctgga
    52141 ctagccttgg gaaatcttct ttttccttgc tatttggtgg tgtcagaaga gttcccaggt
    52201 gcttatcgta gaaagcacta gctccttcaa gcttgctacg atggagtttg agttcattat
    52261 caaagaaagc agtaaatcct tgcttttctc ctgctataaa gtcaaagcgt cccaaaccac
    52321 ctaaaaatag ggtttgctca ggattaagct gataggtctt aggcttgatt tcctttttag
    52381 gactgacata cttgaggttt ttggccgtca agtagtgagc catctggtgg cggtggataa
    52441 ttcccggcgt atcgtaaata taagatccgt cgtcaagcgg aatctctatt ttgtccaagg
    52501 ttgtccctgg aaagcgtgaa gtagtgatga cattctgatc acccgtgatt tcttggataa
    52561 tagcattgat tagagttgat tttccaacgt tggtcacacc gaccacatag acatcgcggc
    52621 ccttacggta gtgttcaatc ttatcaatga cttccttaat ggcatgttta ttttgtgctg
    52681 aagttaggac cacatcgact ggacgaagac cttcttcatg ggcacgtttc atgagccact
    52741 ggctaatctt accagactta actgacttag gaaggatatc ttttttattt cctaccaaga
    52801 ggacatcatt gcccgagacg aaacgtggta aacctgggat gacagatcca ttaaaatcaa
    52861 agatatcaat gacattgacc actaaagcat cactgtctcc cacctcgtgc aagagcttga
    52921 ggaaatcatc gttcgtcaac tggacatctg tgatttcatt gtagtggcgg agacggaaac
    52981 agcgttggca atagacttcg ccagtctcca aacctttttc aagtgccgac tggggggtaa
    53041 aaccaagacc agccttatct gtcgtctgaa tggttgctcc acaaccaata cagagaattt
    53101 cttccatagt taaattcctt ttttatatgt aatcggtccg tacttttcag tgatttttct
    53161 cataacacga cgctcacgag ttcggttaat ctgcgttttg attgagtcat gttggaccaa
    53221 gggtttgact aaaattgacc gaatccctgc acggtgggct gctcgtatat ctgtcatgag
    53281 ctggtcacca accatgacca cttccttttt gtcatagtgg aattccttca tagcacggtc
    53341 aataccaaat gtgaagggct tcaaggccca gtaaacgtaa tcaatcccaa atttctcaac
    53401 tgctcgttga atgcgttttt tggtgttatt tgacactacg ataatgccaa tacccgcgtc
    53461 ccgaaggtca tgtagccatt gcttcatctc tggcgttcca tcagggttgt tccaagcaat
    53521 gagggtatta tccaaatcga ccaaaacagc cttgattccc tgcgcctgca ggcttgggac
    53581 tgtcagatca tagactgctt ccacagcaaa atctggtata taattttcaa tcgccattct
    53641 ggctcctttt cttaactttt tttcgcaatt ttccttagta acaaggataa aacaatccac
    53701 aaacctgcac tagccaaact gatatagagc caagcatggg gctcatctgt taaaggtaag
    53761 ggaacattca ttccgaaaaa gcctgtcacg actgccaaaa cagctagcaa gactgaaatg
    53821 atagtcaagg ttgtcaaatt gtcattcaga ttattgttta gaatattgtt gtaagaggct
    53881 gaaagctgct gtaaaatctg agagattagg tctgtcatgg ataccagctg atgagcctca
    53941 atcatggcat catcaaactg ttctctctca atctcatcaa aactacgata caaggcatga
    54001 ccttgaatat gctctaacaa aatccgattt tgtttggcag ctgccgtcag ataaaccata
    54061 ccagtctcca aatcagacag ggcaaagagg tttttcttgg tagttcgctg gcgcaagagg
    54121 tcattgacct catccctact cttgtccatc tgctcaatga caggatagta ggcattgctg
    54181 atgatttcca gactggcaaa gagaaactta taaatcgaaa gcgtgtcatg gctctccaga
    54241 taacgagtca tctgttcaat gacataggcg ttcttggtat tactaatggt aatcaggcga
    54301 cgatgctcga caataaaggt catgggaaaa gtctcatagt aggccttgtc cttttttacg
    54361 tctaggacat tatagataaa ggtaaccgtc ccactttcac ggtggtagtc catgtgggcg
    54421 cgctcgtttc tatccagtgc gtattcaatg gtttccttgt ccaaaccgta aatttcagaa
    54481 aggtcttcta gtttattcaa ctttcctagg tctaggtcta tccaggtaca accattgccc
    54541 aactgctttt ctaaaaccat cttttctcct tattaaactc cctctattgt accacaaatt
    54601 acttaaaata acaggtctag tctgtgcgtg agaaattgct aacaactgtg atatttcggt
    54661 tagggacaaa gtgaagggct tgctcctcgc tacgaagctg tgtcgtacga attcccacac
    54721 tgacaacctt acccgataca gtaatcggtc catttgtcag aacgacctca tctcccacat
    54781 ccagttgacg ttcaaagagg atgaaaaagc cattgatgac atcagacaga aagccttggg
    54841 ctcccatacc aatcgctacc ccagcaatac cagctccagc cagcaaacta gaaactggca
    54901 aacctaaaat cgacaaaatg cagtagagta aaaagaaata tagcgtataa ttaaacacat
    54961 tttctagtaa acgtgagatg gttttttggc gtccaacatc atgacgagac atttttagag
    55021 aaggtttgac aattctctgc accatggtat gaagcatttt tttagcaata taaaatacaa
    55081 tcaaaagcag taaaagagaa atgaccttgg ttagaatatt ctcgataatg gttgtcacat
    55141 ctagcttttc aatataagcc tgaataaatt tttgcatata aaactccttt atttcattat
    55201 accatatttt catcagtctc attctccata aatattcaaa aaatagacag aaaattcttg
    55261 attttgtcta tcatttatac tataatcata atcactacac aaaaaggaga ttattatgaa
    55321 aagaatcatt catgcttgga ataaggcaag ccttgtcaag cgtatcttga ttggtatgct
    55381 ttgtggggaa tcctaggact gacccttcct aatctctcag gaattgggct actcggggat
    55441 ttattcgttg gaggtctaaa agctgttgct cctatcctag tctttgccct cgttgccaat
    55501 gccctttccc aacatcaaaa gggacaagat agcaatatga aaactgttgt tttcttgtat
    55561 atcctatgat agggactttc gccgctgctc ttgtagctgt actagcaagt ttcatcgtcc
    55621 ctattgaaat taccctaaat agtgccaata ctgaaattgc accaccagat gggattgggc
    55681 aggtcctcag caacctcttg ctcaaactgg ttgacagccc agtcaacgcc ctgcttactg
    55741 ctaactatat tggaatctta tcttgggcag tcatttttgg aatcgctatg agagaagcca
    55801 gtaaaaccag taaaaatagt aaagaattgc taaaaactat cgctgacgtg acttctaaaa
    55861 ttgtcgaatg gatcatcaat ctggctccat ttggaatcct tggtcttgtt tttaaaacca
    55921 tttctgacaa gggagtcgga agccttgcca actacggtat tttattggtt ccattagtaa
    55981 cgactatgct ttttgttgcc cctgtggtca accctttgat tgccttcttc tttatgagac
    56041 gcaatcctta ctctctagtt tggaactgcc tccgtgtcag cggtgtgaca gcctttttca
    56101 ctcgtagttc tgcgactaac attcctgtca acatgaaact ctgccatgac cttggactca
    56161 acccagatac ctattctgtt tctatcccac tcggttctac tatcaatatg gctggagtag
    56221 cgattaccat taaccttttg acccttgtta cagttaacac tcttggaatt cctgttgact
    56281 ttgccacagc ctttgtcctc agtgtggtag cagctatctc agcctgtggt gcttcaggta
    56341 ttgccggagg ttccctcctt cttatcccag ttgcttgtag ccttttcggt atttctaacg
    56401 atattgccat acaagttgtt ggggttggtt ttgtgattgg tgtcatccaa gactcatgtg
    56461 aaacagccct taactcttct acagatgtcc tctttaccgc cgttgccgaa tacgcagcaa
    56521 cccgtaaaaa ataactcatc aaggcaagcc tgctcatgtc ttgtctttta cgcttttatt
    56581 ctaacttatt aggaaattct tatgtctatt agccaacgta cgaccaagct catcttagct
    56641 acctgtcttg cctgcctgct tgcttatttt ctcaatcttt cgtcagcagt ttcagctgga
    56701 attatcgctc tcttgagcct atctgatacg cgtagaagta ctttaaaact ggctcgcaat
    56761 cgtctttttt ctatgcttct agctctggct atcagtgttc tagcttttca cttgagcgga
    56821 tttcatatct ggagtctcgg cctctatctg gccttctacg ttcctttagc ctacaagatg
    56881 ggctgggaaa ttggcatcac accaagcacg gttttggtta gtcatctctt ggttcaagag
    56941 tcaacctctc cagaccttct agtcaatgaa ttccttctct ttgctattgg tacaggattt
    57001 gccttgcttg ttaatctcta tatgccttca cgagaagagg aaatccagca ctaccacacg
    57061 ctggtggaag aaaagttaaa agatatcctc cagcgcttca aatactattt atccagagga
    57121 gacggacgca accgagcaca gctggtagca gaattagaca cgcttttgaa agaagccctc
    57181 agactggtct atttggatca ctctgaccac ctctttcacc agaccgacta ccatatccac
    57241 tactttgaga tgagacaacg acaaagtcgt atcctgagaa acatggccca acagattaac
    57301 acttgtcacc ttgccgccag tgaaagcctg attttagcgc agctcttttc aaaaattgct
    57361 ggtcaactga gccagaccaa tcctgcttct gatttgctag atgaaattga acgttatctg
    57421 gaagtcttcc ggaaccgcag tctgcccaag acaagagaag agtttgaaac ccgcgccacc
    57481 cttcttcaac tcctacgtga agccaaaacc ttcatccaag taaaagttga tttttaccaa
    57541 aaatatggac agtaaaaaat aaaaccacta aaaaacagag cttgccagca ttcttgaaaa
    57601 gtagtataat tattgcatgc gcaatcatct tgtgacgcag agactgcccg ttaatggact
    57661 ttcttctatt ttatgactct aaaaagaaag gagacactat gacctatttg gaaaaatggt
    57721 ttgacttcaa tcggcgtcag aaagaaattg aaagtctctt ggaagagacc attgcccaac
    57781 agagcgagca aagtctgacc ttgaaagagt tctacctgct ctactatctg gacttggctg
    57841 aagaaaaatc tctacgccag attgacctgc cagataagct tcatctgagc ccgagcgcag
    57901 tttctaggat ggtggctcgc ttggaagcta aaaattgcgg tctactcagt cgcatgtgtt
    57961 gccatcaaga tagacgctct agttttatct gcctgacaaa tgatgggcaa aagacactgg
    58021 cctcgctaca aaagactgtc gaagaaagct tggaagcagg tttggatttc ttgatttaag
    58081 atgattttaa aaaaagttgc gtgcgcaatc atttttcttg acattctctt ttataaggag
    58141 taaaataaag ttagcattaa acaaaggagt tttaaatatg attgaaatta cctatataga
    58201 tgccagcaag aacgaaagaa ctgtgacttt cgagtcttat gaagactttg aacgttcgca
    58261 acaagcttgc cttatcggcg tcgcagacta ctaccctgtc caaaaattaa cttacaaggg
    58321 tcataatttg gactaccatg ggacttatgg agatatcttc ttctatctca tgaaacaaga
    58381 tttaagccaa tataactaaa aaaaggagaa atacaatggc aaaagcaatt acagatgcaa
    58441 cattcgaaca agaaacaaaa gacggtttgg tcttagtaga cttctgggca acttggtgtg
    58501 gtccatgtcg tatgcaaggt ccaatcttgg acaaattgtc tgaagaactt tcagaagatg
    58561 tcttgaaaat cgttaaaatg gacgttgatg aaaatccaaa cacagctcgt gcttttggaa
    58621 tcatgtctat tccaactctt ctcttcaaaa aagacggcca agttgtcaaa caggttgcag
    58681 gtgttcacac agcagaacaa atcaaggcca tcattgctga attgagctaa tcaaacgaga
    58741 gaccaagtac tttctttggt ctcttttttc ttgccctttg ccatttttca aaaaatatgc
    58801 tagactatag tatattgaaa ctagaatagt acacctctac ttctaaaaca ttgttagaaa
    58861 tcgatttgac tgtcctgatc gatttgtcct gttcttattt cattttacta tataagtaga
    58921 atattacgat gtttgggata tgccatcgct aaaaaaacct cgatttggta ttttttagct
    58981 ccctaatggg agctttttgc gtgctctgaa catttcccat tttggaagga gtactataaa
    59041 acgtcaatca gccttggtcg tctttagtgg cggtcaagat tcaacaacct gccttttctg
    59101 gaccatgcaa cactatgaaa cagtcgaagc tgtcaccttt gcctacggcc aacgtcatca
    59161 cctcgaaatt caaattgcta gagaaatcgc taaggaacag ggcattcgtc accatatcct
    59221 cgatatgtct ctgctgggac aaatcactgc tcaaccagac tttgcgacga ttcatatttc
    59281 ctacattcct gacaagctct gtgtcgagtc aaaatccctc aaactatatc tatttagcta
    59341 ccgaaatcac ggagatttcc acgaaaactg tatcaacacc atcgggaaag acttggtcaa
    59401 cttgctagac cctcgctatt tagaagtctg gggaaaattc actccgcgcg gtggcatttc
    59461 aatcgacccc tactacaact acggtaagca aggaactaag tatgagggct tggcagaaca
    59521 acgcctcttc caacacgacc tttatccaga gaaaattgac aaccgctaaa ctcatactca
    59581 atgaaaatca aagagcaaac taggaagtta gccgcaggtt actcaaagca gtgtttgagg
    59641 ttgtagataa gactgacgaa gtcagcttaa aacattgttt tgaggttgta gataagactg
    59701 acgaagtcag atcaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagatcaa
    59761 aacactgttt tgaggttgtg gatagaactg acgaagtcag at
//
LOCUS       DAVLVZ010000003        58043 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000003 DAVLVZ010000000
VERSION     DAVLVZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58043
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.003"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            65..1594
                     /gene="rlrA"
                     /locus_tag="VYN77_000142"
     CDS             65..1594
                     /gene="rlrA"
                     /locus_tag="VYN77_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HEV1557684.1"
                     /translation="MLNKYIEKRITDKITILNILLDIRSIELDELSTLTSLQSKSLLS
                     ILQELQETFEEELTFNLDTQQVQLIEHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQ
                     SFNEFTQKEYISIATGYRVRQKCGLLLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFG
                     IEIYDLNDGSMDWVTHMIVQSNSQLSHELLEITPDEYVHFSILVALTWKRREFPLEFP
                     ESKEFEKLKNLFMYPILMEHCQTYLEPHANMTFTQEELDYIFLVYCSANSSFSKDKWN
                     QEKKTHTIQLILQHTRGKHLLSKFKNILGNDISNSLSFLTALTFLTRTFLFGLQNLVP
                     YYNYYEHYGIESDKPLYHISKAIVQEWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLP
                     AIPIFIILNNQADVNLIKSIILRNFTDKVASVTGYNILISPPPSEEHLTEPLIIITTK
                     EYLPYVKKQYPKGKHHFLTIALDLHVSQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYL
                     L"
     gene            1859..3121
                     /locus_tag="VYN77_000143"
     CDS             1859..3121
                     /locus_tag="VYN77_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1557685.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGGFPSLIHLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTVYRKLKQFHFQEDYTTLPEILSWDE
                     FSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSGSY
                     IPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFILK
                     DSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNTDQFW
                     GLIQDTLPHLNRTFKTTLSTFICYKNYITNAIELPYSNAKLEATNKLIKDIKRNAFGF
                     RNFENFKKRIFIALNIKKERTKFVLSQA"
     gene            complement(3259..5583)
                     /gene="pflB"
                     /locus_tag="VYN77_000144"
     CDS             complement(3259..5583)
                     /gene="pflB"
                     /locus_tag="VYN77_000144"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1557686.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            5977..7038
                     /gene="dinB"
                     /locus_tag="VYN77_000145"
     CDS             5977..7038
                     /gene="dinB"
                     /locus_tag="VYN77_000145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1557687.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(7185..8030)
                     /locus_tag="VYN77_000146"
     CDS             complement(7185..8030)
                     /locus_tag="VYN77_000146"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1557688.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            8286..9145
                     /locus_tag="VYN77_000147"
                     /pseudo
     CDS             8286..9145
                     /locus_tag="VYN77_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9446..11335)
                     /locus_tag="VYN77_000148"
     CDS             complement(9446..11335)
                     /locus_tag="VYN77_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1557689.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFRERTVEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVKEKEQSKQLLTWIFPSILSISLLLSICFYFIYRRKTAPSIK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            11473..13038
                     /locus_tag="VYN77_000149"
     CDS             11473..13038
                     /locus_tag="VYN77_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1557690.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQITGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMVQVEPGF
                     KTGEEDTAIAIGLRKDDTRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTITGLIIGLAIGVFRTAPLSENKVIYS
                     LQKLVGWILNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            13038..13778
                     /locus_tag="VYN77_000150"
     CDS             13038..13778
                     /locus_tag="VYN77_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1557691.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(13887..14102)
                     /locus_tag="VYN77_000151"
     CDS             complement(13887..14102)
                     /locus_tag="VYN77_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557692.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
     gene            complement(14972..16222)
                     /gene="ilvA"
                     /locus_tag="VYN77_000152"
     CDS             complement(14972..16222)
                     /gene="ilvA"
                     /locus_tag="VYN77_000152"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1557693.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(16272..16628)
                     /locus_tag="VYN77_000153"
     CDS             complement(16272..16628)
                     /locus_tag="VYN77_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1557694.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(16866..17102)
                     /locus_tag="VYN77_000154"
     CDS             complement(16866..17102)
                     /locus_tag="VYN77_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557695.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(17209..18231)
                     /gene="ilvC"
                     /locus_tag="VYN77_000155"
     CDS             complement(17209..18231)
                     /gene="ilvC"
                     /locus_tag="VYN77_000155"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1557696.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPSLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(18297..18773)
                     /gene="ilvN"
                     /locus_tag="VYN77_000156"
     CDS             complement(18297..18773)
                     /gene="ilvN"
                     /locus_tag="VYN77_000156"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1557697.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(18766..20466)
                     /locus_tag="VYN77_000157"
     CDS             complement(18766..20466)
                     /locus_tag="VYN77_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1557698.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(20667..22334)
                     /locus_tag="VYN77_000158"
     CDS             complement(20667..22334)
                     /locus_tag="VYN77_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1557699.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(22337..22702)
                     /locus_tag="VYN77_000159"
     CDS             complement(22337..22702)
                     /locus_tag="VYN77_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1557700.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(22859..23047)
                     /locus_tag="VYN77_000160"
     CDS             complement(22859..23047)
                     /locus_tag="VYN77_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1557701.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(23163..23339)
                     /locus_tag="VYN77_000161"
     CDS             complement(23163..23339)
                     /locus_tag="VYN77_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1557702.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENITQDLI"
     gene            complement(23450..23647)
                     /locus_tag="VYN77_000162"
     CDS             complement(23450..23647)
                     /locus_tag="VYN77_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1557703.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(24075..25619)
                     /locus_tag="VYN77_000163"
     CDS             complement(24075..25619)
                     /locus_tag="VYN77_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1557704.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            25832..26134
                     /gene="gatC"
                     /locus_tag="VYN77_000164"
     CDS             25832..26134
                     /gene="gatC"
                     /locus_tag="VYN77_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1557705.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVVEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            26134..27600
                     /gene="gatA"
                     /locus_tag="VYN77_000165"
     CDS             26134..27600
                     /gene="gatA"
                     /locus_tag="VYN77_000165"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1557706.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKHSEETIYQVAAAFEATTGYHKQQPVIFGGDN"
     gene            27600..29042
                     /gene="gatB"
                     /locus_tag="VYN77_000166"
     CDS             27600..29042
                     /gene="gatB"
                     /locus_tag="VYN77_000166"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1557707.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAIADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            29171..29731
                     /gene="efp"
                     /locus_tag="VYN77_000167"
     CDS             29171..29731
                     /gene="efp"
                     /locus_tag="VYN77_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1557708.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            29753..30142
                     /locus_tag="VYN77_000168"
     CDS             29753..30142
                     /locus_tag="VYN77_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1557709.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGHGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            30135..30557
                     /gene="nusB"
                     /locus_tag="VYN77_000169"
     CDS             30135..30557
                     /gene="nusB"
                     /locus_tag="VYN77_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1557710.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(30606..30833)
                     /locus_tag="VYN77_000170"
     CDS             complement(30606..30833)
                     /locus_tag="VYN77_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1557711.1"
                     /translation="MLSGLIFGLSHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRL
                     TILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(31324..31509)
                     /gene="briC"
                     /locus_tag="VYN77_000171"
     CDS             complement(31324..31509)
                     /gene="briC"
                     /locus_tag="VYN77_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1557712.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(31668..32435)
                     /locus_tag="VYN77_000172"
     CDS             complement(31668..32435)
                     /locus_tag="VYN77_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1557713.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(32432..33298)
                     /locus_tag="VYN77_000173"
     CDS             complement(32432..33298)
                     /locus_tag="VYN77_000173"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1557714.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(33335..34702)
                     /gene="accC"
                     /locus_tag="VYN77_000174"
     CDS             complement(33335..34702)
                     /gene="accC"
                     /locus_tag="VYN77_000174"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1557715.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(34714..35136)
                     /gene="fabZ"
                     /locus_tag="VYN77_000175"
     CDS             complement(34714..35136)
                     /gene="fabZ"
                     /locus_tag="VYN77_000175"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1557716.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(35133..35618)
                     /locus_tag="VYN77_000176"
     CDS             complement(35133..35618)
                     /locus_tag="VYN77_000176"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1557717.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(35621..36856)
                     /gene="fabF"
                     /locus_tag="VYN77_000177"
     CDS             complement(35621..36856)
                     /gene="fabF"
                     /locus_tag="VYN77_000177"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1557718.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(36878..37609)
                     /gene="fabG"
                     /locus_tag="VYN77_000178"
     CDS             complement(36878..37609)
                     /gene="fabG"
                     /locus_tag="VYN77_000178"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1557719.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(37643..38563)
                     /gene="fabD"
                     /locus_tag="VYN77_000179"
     CDS             complement(37643..38563)
                     /gene="fabD"
                     /locus_tag="VYN77_000179"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1557720.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEATPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(38556..39530)
                     /gene="fabK"
                     /locus_tag="VYN77_000180"
     CDS             complement(38556..39530)
                     /gene="fabK"
                     /locus_tag="VYN77_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1557721.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(39649..39873)
                     /locus_tag="VYN77_000181"
     CDS             complement(39649..39873)
                     /locus_tag="VYN77_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1557722.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(39933..40907)
                     /locus_tag="VYN77_000182"
     CDS             complement(39933..40907)
                     /locus_tag="VYN77_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1557723.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(40907..41341)
                     /locus_tag="VYN77_000183"
     CDS             complement(40907..41341)
                     /locus_tag="VYN77_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1557724.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(41436..42221)
                     /locus_tag="VYN77_000184"
     CDS             complement(41436..42221)
                     /locus_tag="VYN77_000184"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1557725.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            42546..43910
                     /locus_tag="VYN77_000185"
     CDS             42546..43910
                     /locus_tag="VYN77_000185"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1557726.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRELTPIKEEEILRQLVQKTKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGK
                     SKED"
     gene            43913..44284
                     /locus_tag="VYN77_000186"
     CDS             43913..44284
                     /locus_tag="VYN77_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1557727.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            44497..45771
                     /gene="serS"
                     /locus_tag="VYN77_000187"
     CDS             44497..45771
                     /gene="serS"
                     /locus_tag="VYN77_000187"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1557728.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(45797..46696)
                     /locus_tag="VYN77_000188"
     CDS             complement(45797..46696)
                     /locus_tag="VYN77_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1557729.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLTE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(46805..47353)
                     /locus_tag="VYN77_000189"
     CDS             complement(46805..47353)
                     /locus_tag="VYN77_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1557730.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(47571..48893)
                     /locus_tag="VYN77_000190"
     CDS             complement(47571..48893)
                     /locus_tag="VYN77_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1557731.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(48984..49072)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(49236..51572)
                     /locus_tag="VYN77_000191"
     CDS             complement(49236..51572)
                     /locus_tag="VYN77_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1557732.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKEIELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDAFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(51676..52224)
                     /locus_tag="VYN77_000192"
     CDS             complement(51676..52224)
                     /locus_tag="VYN77_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1557733.1"
                     /translation="MISLLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(52221..52523)
                     /gene="zapA"
                     /locus_tag="VYN77_000193"
     CDS             complement(52221..52523)
                     /gene="zapA"
                     /locus_tag="VYN77_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1557734.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            52610..53491
                     /locus_tag="VYN77_000194"
     CDS             52610..53491
                     /locus_tag="VYN77_000194"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1557735.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            53495..54109
                     /gene="lepB"
                     /locus_tag="VYN77_000195"
     CDS             53495..54109
                     /gene="lepB"
                     /locus_tag="VYN77_000195"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1557736.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            54237..56603
                     /locus_tag="VYN77_000196"
     CDS             54237..56603
                     /locus_tag="VYN77_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1557737.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPVDFTQKKADRSYFEITSGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(56651..57934)
                     /locus_tag="VYN77_000197"
     CDS             complement(56651..57934)
                     /locus_tag="VYN77_000197"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1557738.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     EDGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        17193 a        13082 c        10922 g        16846 t
ORIGIN      
        1 acaattagtt tcttttgtat tataataatt aaaattatct atatgcaact aagattcggt
       61 aactatgcta aacaaataca ttgaaaaaag aattacagat aaaattacca ttttaaatat
      121 attattggat attcggtcta ttgaactaga tgaactgtct actttaactt cattacaatc
      181 taaaagtctc ctaagcattt tgcaggaatt acaagaaaca ttcgaggaag agttgacatt
      241 taatttagat acgcaacaag ttcaattaat cgaacaccat tctcatcaaa cgaattatta
      301 cttccatcag ctttataacc aatcaactat tttaaaaata ctccgcttct ttctactaca
      361 aggtaatcaa agctttaacg aatttacaca aaaagagtat atttccatcg caacaggcta
      421 ccgcgtacga caaaagtgtg gcttgttact acgttcagta ggtttggacc ttgtgaaaaa
      481 tcaagtagtt ggtcctgaat atcgcattcg ttttttaatt gcactcctcc agtttcattt
      541 tggtattgag atttatgatt tgaatgatgg aagtatggat tgggtcacac atatgattgt
      601 gcagtctaat tctcaactca gtcatgaact gttagaaatc acacctgacg agtatgttca
      661 ttttagcata ctagtggcac tcacttggaa acgtcgggaa tttccacttg agttccctga
      721 gtccaaagag tttgaaaaat taaaaaacct ctttatgtat ccgattttga tggaacattg
      781 ccaaacttac ttagagccac atgccaacat gacttttact caggaagaac tggattatat
      841 ctttttggtt tattgttctg ctaattcttc gttttccaag gacaaatgga atcaagaaaa
      901 aaagactcat actattcaac tgattcttca gcatacaaga ggaaagcatc tactatccaa
      961 gttcaagaat atcttaggaa atgacatttc gaattctctt tcttttttaa cagcactgac
     1021 tttcttaaca agaacgtttt tgtttggttt acaaaatcta gttccctatt ataattatta
     1081 cgaacattat gggattgaaa gcgacaagcc actgtatcat atttcgaaag caattgttca
     1141 agagtggatg actgagcaga agattgaagg agttatcgac cagcatcggc tgtacctttt
     1201 ctcactctac ttgacagaaa ccatcttttc ctcactacct gctatcccga tttttatcat
     1261 tttaaataat caagcagatg taaacttaat aaagtccatt attttacgga attttactga
     1321 caaagttgcc tctgttacag gatacaatat tctaattagt cctcctcctt cagaggagca
     1381 tttgacagag ccattgatta ttattacaac gaaagaatac ctgccttacg ttaaaaagca
     1441 gtatcccaaa gggaaacatc actttttgac gattgcctta gatttgcatg ttagccaaca
     1501 gcgtctcatc tatcagacaa ttgtagatat ccgaaaagag gcttttgaca aacgggtcgc
     1561 aatgattgct aaaaaggctc actatttgtt ataagttcat cgtactgtct acacacaaaa
     1621 aggggcttcc taagccatct ctattcttat ccccatattt cccagttttg ggtaaatctg
     1681 aacagaaaaa cacttctgct ctctagtttc tgctagtgaa caaaagtgtt ttttctggct
     1741 ctataatttc tgtagtgggt aaatcccact gtggagatta tagggctttt tgagtatcaa
     1801 aaaagtccca taagacttat aatgaaaagc gcctaaacaa ctcattagaa agtatcttat
     1861 ggaacacatc aatcatacca cattactcat cggaataaaa gacaaaaaca tcaccttaaa
     1921 taaagccatt cagcacgata cccatatcga agtcttcgct acacttgatt atcacccacc
     1981 taaatgtaaa cactgtaaag gaaaacaaat caaatacgac ttccaaaagc cttctaaaat
     2041 cccttttatc gagattggtg gtttccctag cctcattcat ttgaaaaaga gacgatttca
     2101 atgcaagtcc tgtcggaaag tcactgtagc tgaaacaact ctcgttcaga aaaattgcca
     2161 aatctcggaa atggtgagac agaaaattgc ccaactccta ctcaacagag aggctcttac
     2221 acatatcgct tctaaattag ccatctctac ctctacctct accgtctatc gtaagctcaa
     2281 gcaatttcat ttccaagagg attacaccac tttgcctgaa atcctctcct gggatgaatt
     2341 ctcctaccag aaggggaaat tggctttcat tgctcaggat ttcaacacta agaaaatcat
     2401 gaccattctt gataacagac gtcaaacaac catccgaaat catttcttca agtactcgaa
     2461 agaagctaga aaaaaagtta aagtcgtcac tgttgatatg tctggaagtt atatccctct
     2521 cattaagaaa ttatttccca atgctaaaat tgttctcgat cgtttccaca ttgtccaaca
     2581 catgagcagg gctcttaatc agactagaat caacatcatg aagcaatttg atgataaatc
     2641 tctggaatac agagctctta aatattactg gaaatttatc ctaaaagata gtcggaaact
     2701 ctctcttaag cctttctatg ctagaacttt cagagagacc ttaactccta gggagtgtct
     2761 gaagaaaatc tttactctag tacctgaact taaagattac tatgacctgt atcaactact
     2821 cctatttcat ctacaagaga agaatactga ccagttttgg ggcttaattc aagacacgtt
     2881 acctcatctc aaccgcacct ttaaaaccac tttgagcaca tttatttgct ataaaaacta
     2941 catcactaac gccattgaat tgccttattc taacgctaaa cttgaggcca ctaacaaact
     3001 catcaaagac atcaagcgca atgcctttgg ttttcggaac tttgaaaact tcaaaaaacg
     3061 gatttttatc gccctgaaca tcaaaaaaga aaggacgaaa tttgtccttt ctcaagctta
     3121 gctttttttc aacccactac agttgacaaa gagcccaaaa acattgcata ttgcgccgaa
     3181 ggaattgctc atactatccc aagcacaaaa ccctcagtcg tttgaccgag ggttcctttt
     3241 tattattcaa gaacttgatt agctcaatgc atccaaggcg tcatccattg aaagaacttc
     3301 gtggaagaca cgttgtgtca attcagtttt ttgttctgga gtgaggtatt tagtgtttac
     3361 acagtatcca gagatacgta cgataacgtc ttcgcctgac atgatttttt cgtaaacatc
     3421 gttcaagtcc ataacgttca agttaacgtg ttgtccaccg ttttcgaagt aaccatcaag
     3481 gattgttacc aagttatcaa cttgttcatc acgagtctta ccaagagcgc gaggtgatac
     3541 ttgtgtagtc aatgagatac cgtcagctgc ataactaaag tcaaggctag aaagtgagtt
     3601 caagttttgc aaccaaccac ctttagcttt gttagatggg ttagcacctg gtgagaagaa
     3661 ttcaagttta gacaagttca cagaaccatc ttcgttgagg tatacacctt tgtgaactgg
     3721 tgagttacca gtttgtttag agtaagcaac gttagatgtg attgtcaaaa gtgatactgt
     3781 agcttctgcg tctttgtata gtttgtggct acgtagacga gttgtgtaag cttcgatcaa
     3841 ccattctgcc aattcgtttg aacgtgggtc atcttcaccc cagcgtgggt agtcaccgat
     3901 tgtttcgtaa tcgtagatgt agccatcttc gtcacggatt ggtttaactg tagcgtattt
     3961 gatagctgac aatgtatcaa cagtgttagc aaatccacag ataccgaatc ccatgttggc
     4021 acgttgttta gttggcaaga aggccatttg aacagcttcg tagttgtacc tatcagtcat
     4081 gtagtggatg atgttcaagg catctacgta agtgtcagtc aaccagtcaa gagatttttc
     4141 aaagttcgct ttaactgatt caaattcaag aacttcgtca cggattggtt cgatatcaaa
     4201 tactttgtag tctttgtgaa catcgtcgta accaccattc aaaccagtaa gaagggcttt
     4261 aagaacgttt acacgagcac cgaagtactg gatgttgtgg cgttgttctt cattttctgg
     4321 atcaagtgga gacacacagc atgagataca gctcatttca ccatatccgt ctttagccat
     4381 tgttgttaca ccttcgtatt ggatagaaga gtgtttgtgg ctcatgtgca tacagtagcg
     4441 acggaagttg tatggcaatt tgtcagtcca aagaactgtc aagtttggtt ctggtgagtt
     4501 accgatgttg tcaagagtgt tcaagaaacg gtagtccatc ttagtaacac ggtgacgacc
     4561 gtcgttaccc ataccagcca tagaagttgt gataaaggtt gggtcacctg agtacaattg
     4621 gtcataagct tttgtacgag caaatttaac tgtacgaagt ttcataacga aatcatcaac
     4681 gaattcttgg atttctgatt cagtaaatgt accacgagca aggtcacgtt ctgcaaagat
     4741 gtccaatacg attggtacac gacctagaga tgtagcagca ccgttaatca cacggcagac
     4801 agccatgaaa gcaatgttaa cccattggat tgcttctttc acgttcatcg ctggtttgcg
     4861 aacatcaacc ccgtaaaggt cacccaggcg aacaacttgt tgcaatgctt ggtattgaag
     4921 gtttacttct tcacgaagac ggattgtttc ttcatcgatt tctttgattg cattccagtc
     4981 atttactttt tcttgcatca agtagtctgc accgtaaaga gcaagacgtg cgtaaacacc
     5041 gatgatacgt ccgcgtgagt atgcatctgg aagaccagtt acagtgtgtg cgtgacgagc
     5101 gcgacgaata tttgaagtgt aggcacggaa aataccgtcg ttaactgttg ttacatattt
     5161 agtgaagatt tcgtgaacag ctgggtctgg ttcgtatcca ttttctttca aagtagtttc
     5221 agccatacgg ataccacctt ttggcatgaa gttcaatttg aagagttcat cgttttggat
     5281 accgaagata acttcatttt ctttgtcgat aaatccagca gggatatcag cgatagatgt
     5341 tggacgagtg tccattggga aacgagtttc ttcgtagtgt gctttagttt cttctacaat
     5401 tttcttgatg tgaagtgaac gctctgttgg tcctgcaagg aagctttcgt ctccatcata
     5461 aggtgtgtag ttagcttgta caaatcgtga tacacttgct ttttctttcc aatctacgcc
     5521 tttgaagcct tcccaagctt tgtcaaaaat atcttgtgct tcaacaactg tcttaacaac
     5581 catattaatg tcctcttttt tctagtaaca gtcatctgtt acattcataa gacaagtata
     5641 ccatatagta accgatttca acaagtgaaa attccctatt tttacacttt cttttctaaa
     5701 acagtctata ttttatttca aactgtatta tatttttgaa aaaataaagt ccttttttct
     5761 ttttttcaga aaaaagggta taataaaaga aaataagcag taacactcaa tggaaatcga
     5821 aaagcaaact aggaagctag ccgcaggctg ctcaaagcac tgctttgagg ttgtaaatag
     5881 aactgacgaa gtcagctcaa aatactgttt tgaggttgca gatggaagct gacgtggttt
     5941 gaagagattt ttgaaaagta taaaaaggtg ctaggcatgt tgatttttcc tttgttaaat
     6001 gatttgtcaa gaaaaatcat ccatattgac atggatgcct tttttgctgc ggtggaaatc
     6061 aaggataatc ctaaactcag aggaaaacct gtcattattg gaagcgaccc tcgacaaaca
     6121 ggtgggcggg gagtcgtttc tacctgtagc tatgaggctc gagcttttgg tgtccattct
     6181 gccatgagtt ccaaggaagc ttatgaacgt tgtccccagg ccgtctttat ctcaggaaat
     6241 tatgagaaat acaaagctgt gggactccag attcgagcta tttttaagcg ctatacagat
     6301 ttgattgaac ccatgagtat tgacgaagcc tatttggatg tgacagaaaa taaactcggt
     6361 atcaagtcag cggtcaaaat tgctcgcctc attcaaaaag atatctggca agaactccat
     6421 ctaactgctt ctgccggtat ttcctataac aagttcttag ctaaaatggc gagtgattat
     6481 caaaaacctc atggtttgac agtgattcta cctgaacagg ctgaggattt tctcaaacaa
     6541 atggatattt ccaaatttca tggagtagga aaaaagacag tagaacgtct tcatcaaatg
     6601 ggcgttttta ctggtgctga tttacttgaa gttcctgagg tgaccctaat agaccgtttt
     6661 ggtagactag gctatgatct gtatcgaaag gctcgtggca ttcacaactc tccagtcaaa
     6721 tccaatcgca tccgtaaatc aatcggcaag gagaaaacct acgggaagat tctccgtgct
     6781 gaggaagata tcaaaaaaga gctgactctt ctatcagaaa aagtcgctct caatctacat
     6841 caacaagaaa aagctggaaa aattgtcatt ttgaaaatcc gctacgagga cttttcaact
     6901 cttaccaaac gaaaaagtct tgctcaaaaa acacaagatg ctagtcagat aagccaaata
     6961 gccctgcaac tctatgaaga attaagcgag aaagaaagag gtgtccgcct attgggaatt
     7021 accctgactg gattttaaag ctcaatgaaa atcaaagaac aaactaggaa gctagccgca
     7081 ggctgctcaa aacactgttt tgaggttgca gataaagctg acgcggtttg aagagatttt
     7141 ctaagagtat aaaaccttga agagttgccc cttcaaggtt tttcttatac aaataaacgg
     7201 acaaaactgt aaagtagcaa aacactacca agcacgatac ggtatttacc aaaaagggtg
     7261 aagtcgtgtt ttttcacata gctggtcaag aagcgaatag ccaccatgct gaccgcaaaa
     7321 gctactccca tcgcgaccaa gagcaaaaac aattgcccaa agctcaagag ttctccggct
     7381 ttcacaaatt tgaaaatctt taaggcacta gctccaaaca taacgggaat cccaagatag
     7441 aaggtaaatt ctgtcacaac tgaacgactg gttccattta acaaaccacc gacaatcgtt
     7501 gcacctgaac ggctagtccc tggtaaaaga gcaagaactt ggaagagtcc gatatagaaa
     7561 gcggtcgtat aaggaagctt gtccaactct gttacacttg gctcgatagc acgcgcttta
     7621 ttgcgctttt ccaaatagat gaaggcaacc ccgtagataa tcaacatgag agcaactgaa
     7681 accatgttat ggaagtgggt atcaaaccaa tcatcaaatt taaagacacc aagtaaaggt
     7741 aaagtagcaa tcaagacctt caaccatagt ctccaagtct tacgaacttc ctgtttgtcc
     7801 ttagtcggtt taaaaggatt gagcttgtta aaataaatca ccataactgc taaaatagca
     7861 ccaagctgaa tcacgacatt aaacatggac ataaaggctt cattttgatt ttggtattgg
     7921 ataaactcct ctgctaaaat caagtgacct gtactggaaa tcggcaacca ttccgtaatt
     7981 ccttcaacaa tcccgaagaa gatagatttt aaaatttcaa taagatacat atatttctcc
     8041 ttatattata tttaacgcag ttagctactg ccaaagccca agtggtatac ttggaataag
     8101 ccactgtgga ttagttcatt tcctccaatc gcttgacgat ttccgaaagg gtctaaccac
     8161 agctttttgt ttcaattggt aatgagtttg ataacgcata gacgttttat ctcgtctaag
     8221 gtaacaaggc aaaaagttcg tggataacca agtagacttg aaatactata tctggaggta
     8281 ctcccatgct ttatgtgggc attgatgtcg ctaaaaataa acacgatgtt acagccttga
     8341 atgttcgagg aaaactgttc ttaaaccact cactttttca aataataaag ctggttttga
     8401 actcttagat ctgtcttttc gacagctcaa ccaagactgt ctcatcgctc ttaaacttct
     8461 ttctgacccc aatcgtgaac aatttcaaca cgataatcgg caagtagaac taaaaatact
     8521 ggctagacat attcatcgtc tcaagagaaa acagtctgat tggaaagtac aatacactcg
     8581 ttgtcttgat atcatctttc ctgagttgga taaaatcgtt ggaaagcatt cagaatatac
     8641 ctaccaactc ttgacatgct accctaatcc tcagaaaagg cttgaggcag gatttgataa
     8701 gctgatagaa attaagcgat tgaccgcttc taaaattcag gatatcctct cagtcgcacc
     8761 tcattctatc ggaacaacat ctcctgctcg tgaattcgaa atcatcaaac attacaagag
     8821 gctcattgac aaggcggaaa catgtgtcaa tgacttgatg gctgagttca actcggtcat
     8881 cacgacggtc actgggattg ggaatcgttt aggggcggtc attttagccg agattcgaaa
     8941 tattcatgcc tttgataatc ctgctcaatt acaagctttc gctggactgg attcttctat
     9001 ttatcagtca ggtcagattg atttagctgg aagaatgatc aaacggggtt cccctcatct
     9061 gcggtgggca ctcatacaag ctgccaaagc atgcgctcgc ttttcacctg cttttaaggc
     9121 ctatcttaag actaagttag aataaggaaa acattacaat gtagccatca tccaccttgc
     9181 aaaaaaactt atccgaaccc tgttttatat cctaaaaaag agctgccatt tgacgaacaa
     9241 aaagtgatgt aatttctgaa attaacacag gattaccttt gaaaaaatgg actttttcaa
     9301 gggctttttg ccgtgctcta aaatcatcgc caaatcatca aattgaaata agaaatagtt
     9361 tagataaaac tattgacttt tcttatagtt tgtctttctt ctatcttcca ttatatcata
     9421 ctttttcagt ctatggtagc tctctttaaa aggctccgat actgccgcca cctccgcctc
     9481 cagagaagcc accgccagaa cttccacttc cagaagatac ggagtaggta cttgctgtat
     9541 ttgcgacact agcataatgg ctcatttgcg cgcttgaatg ataaaacata ctgtgccagc
     9601 cataagctac atagagattg atatctggat tttcaacttg aatctgatga accttcatca
     9661 aatgactaac cttgtccgca tagccaaata aggttgcata gaccaagaga cgattccaaa
     9721 caacaatact ttccaattca gcctgatcca atcgtgcaat ctcacgcaac atattttcaa
     9781 aactggtcca gagatagtag acctcagctc ccgcttcatt tagaacacca tcacgattat
     9841 ctagtcgaag cttccaataa tagaaaacag acaaaactaa ccctagaaaa ccaagtattg
     9901 gcaaagggag gtaaagatag ccatgaacgt ctaaactgta caagaacaaa ccaaatccga
     9961 taaatagggg caagatagtc aagacaccca tacccacttg caaagccttt tccaaaccag
    10021 ttaaaggacg atagtaatct gggagccccc agaaggaaac tcgttttctc actccttctt
    10081 gcatctggtt caatacctct tcaaaagaag atttgagttg aagccctctt gcttgaatcc
    10141 gtttttcatc agaaactttg gctctacgat aaagactatc agataccttg taatccgcaa
    10201 acaaattgga aagagtttct tcttttttac ctgaaaaagc tagatttagg cagtctttct
    10261 caaagcttga caaaccatct tcttttacta gcctcaaacc aactgcatct ccttctgaaa
    10321 tgatagagac attcccacgg tctatcacat ctagcaaggt agcttgaata agttgatcaa
    10381 aggtgaattt tccagctccc ttgaccaagg gactcacttc ctccaaggag gtcgagtaga
    10441 ctgcttctga taaaaccata ggctctaatt ccattggggg ttcatagaga cgatgatttt
    10501 tagcatattt gattgaagga gcggtctttc ttctatagat aaaatagaaa cagatgctca
    10561 ataacaagga gatggaaagg atcgaaggga agatccaagt aagtagttgt ttactttgct
    10621 ctttttcttt aacgatcgaa tcctctatct tattaaactc ttctaaacga ttccctttca
    10681 agccctgatc cctagcgcta gcaaaatcag ttcgcggcca gtaggcatgc aattcaactc
    10741 cacgcttagc cggaagattg tctaaacgaa tagtataatc aaggttactc ttttcaaccg
    10801 ttctctctct aaaaagtttc cctgtatgga aaaagagttt ctcagccccc ttgtctcccc
    10861 ttacatgaaa ttcaaacttt tcaatagact ctgaactatc tgtcagaggt tgccaattta
    10921 attcagcgat atcatcataa aggaaaagta aattttttaa gttccagacg aggtcaactt
    10981 caactatgtc gccctcctga cctggattat agactctcac agtataacca tccgcttctt
    11041 ctgttacttc gctagtcaca tctgctagtt ctgcaccgtt tttcgcggcc tgaatctttg
    11101 gatgagggtc aatgtcaaac ccgctaggca tcttaccagc acgtccaagt cccacgattt
    11161 ggcccttaaa gtcctcctca aactggtaaa ctatcttctg tctaaactct gccgtattgt
    11221 ctgcatgaat atacaaatcc ccttgataag agtttatctt gaaatcaatg gcaaaaacag
    11281 agagtggaag aaggcaaaac aagcctaaca ccagtaagaa aaaagttttt ttcatcaact
    11341 aagccccctt tttatctttc ttatcattat agcatttttt cgtaagtaaa gacttactta
    11401 tgttgaaaaa ttacgcattt ttcgataaaa taggagacag ttatttttta atagatcaga
    11461 ataggagaaa tcatgagaaa aatatactta tctattttca caagtctctt gctgatgcta
    11521 ggacttgtca atgttgctca agccgatgaa tatttacgca tcgggatgga agcagcatat
    11581 gctcccttta actggaccca ggatgatgat agcaacggag ctgtcaaaat cgatgggacc
    11641 aatcagtatg ccaacggata cgatgttcaa atcgccaaga aaatcgctaa ggacttaggt
    11701 aaagaacctt tggttgttaa aaccaagtgg gaaggtctag tccctgccct tacttctggt
    11761 aagattgaca tgattatcgc aggtatgagt ccaactgcag aacgcaaaca agaaattgcc
    11821 ttttcgagca gttactacac tagcgaacca gttctactgg tcaaaaaaga ttctgcctat
    11881 gcaagtgcta aatctttgga tgactttaat ggtgcgaaaa tcacttctca acaaggggtc
    11941 tacctttatg acttgattgc acaaatcaca ggtgctaaaa aagaaacagc catgggagac
    12001 ttcgctcaaa tgcgccaagc tcttgaggct ggtgtcatcg acgcctatgt ttctgaacgc
    12061 ccagaagctc tgactgccga agccgctaac tctaagttca agatggtcca agtagaacca
    12121 ggtttcaaaa ctggggaaga agatacagct atcgccattg gacttcgtaa agatgacact
    12181 cgtattagcc aaatcaatgc cagcattgaa accatttcaa aggacgacca agttgccctg
    12241 atggatcgta tgatcaagga acaacctgcc gaagctacaa caactgaaga gactagcagt
    12301 agtttcttta gccaagttgc taaaattctt tctgaaaact ggcaacaact cttgcgtggt
    12361 gctggtatca ctcttttaat ctctatcgtc ggaaccatca caggtctcat tattggactt
    12421 gccattggtg ttttccgtac tgctcctctc tctgaaaaca aagtcattta cagcctacaa
    12481 aaactagtcg gctggatcct caacgtctac attgaaattt tccgtggtac gccaatgatt
    12541 gttcaatcga tggttatcta ctatggaact gcccaagctt tcggtatcaa ccttgaccgt
    12601 acactggctg ctatcttcat cgtttcaatc aataccggtg cctacatgac tgaaatcgtc
    12661 cgtggtggta tcctagcagt tgacaaggga caatttgaag ctgcgactgc tcttggtatg
    12721 acccataacc agaccatgcg taagattgtc ctacctcagg tagtccgtaa catcctacct
    12781 gcaactggta atgaatttgt catcaatatc aaagatacat ctgtattgaa cgttatctct
    12841 gttgtcgaac tttatttctc aggaaatacc gtggcaacac aaacctatca atacttccag
    12901 acatttacaa tcatcgccgt gatttacttt gtcctcacct tcaccgtaac acgtatccta
    12961 cgatttatcg agcgccgaat ggacatggat acctatacta caggtgctaa ccaaatgcaa
    13021 acggaggatt tgaaataatg acacaagcaa tccttgaaat taaacacctc aaaaaatcct
    13081 atggacaaaa cgaagtgcta aaagacattt cactcactgt ccacaaggga gaggtcatct
    13141 ctatcatcgg aagctctgga agcggaaaat cgaccttcct acgctccatt aacctacttg
    13201 aaacaccaac tgatggacaa atcctttatc atggacaaaa cgtcctcgaa aaaggctatg
    13261 acctcacgca ataccgtgaa aagttaggga tggttttcca atcctttaac ctctttgaaa
    13321 atctcaacgt tcttgaaaac acaatcgtcg ctcagacaac tgtccttaaa cgcgaacgca
    13381 cagaagctga agaaattgcc aaagaaaacc tggaaaaggt cggcatggga gaacgctact
    13441 ggcaagctaa acccaaacaa ctctcaggtg gtcaaaaaca acgtgtggcc attgctcgtg
    13501 ccctttcaat gaatcctgac gccattctct ttgatgaacc aacatcagct ctcgatccag
    13561 aaatggttgg agaggtcctc aaaatcatgc aagatctggc tcaggaaggc ttgaccatga
    13621 ttgtcgtaac ccatgaaatg gaatttgccc gtgatgtctc tcaccgtgtt atctttatgg
    13681 ataagggcgt gatcgctgaa gaaggtaaac cagaagacct cttcaccaat cctaaagaag
    13741 accgaacaaa agagttcctt caacgctatc tcaaataaaa agaaaaggct gcatcaaccg
    13801 tgcagtcttt ttgctgtcct caaaatgaaa aggcagattc tctatctcta ctacttgaag
    13861 gtcaagaatc caccgttatc caaagattaa tcttcaaatt tttcatgatt tttttcatag
    13921 aaatcaatta agcctagggc tgtttcaaat gaaatatttt ttacttttgc acgaccctgc
    13981 gccagagcaa tgatagacat ttcacgcgca ttcgtttctt tagagatacg gtagcctgtg
    14041 attttcttat cacgaaccca gccaacaact gattctactt tttcaaagtt tgacttagcc
    14101 atgtttttct cctattttct cctttataat atttaattgt atacgttttt actacttttg
    14161 tcaataaaaa aacatatatt caacgaaaat aattagttca gaattttctt acttcctttt
    14221 taaatccgat aatatatagg ttttgctagc ctctacacca taaatatttg ctaataaaaa
    14281 tactgtatta ttaccctctt aaggtgcgaa attattcaag ttcttaaaaa atgcttctct
    14341 atttccgtaa gatagcttat ttcttcgatt taagaactga agatgatagt tctaaccttt
    14401 agaaatccaa gccttcacta gctcaaaaac tcctatcttt cttcctcaat aaatcatctg
    14461 agagataggt attccttatg tccttcaaat tcatcactcc tttggatatt gaggtgctag
    14521 cctagaagca gacacccgat tatctaaacc atccttaata gagatgacaa gacagataag
    14581 ctcagttaat tacttatata tctttttaat atggtatatt tgtcaacaaa aagaaatcaa
    14641 atcatacctt atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaagttgct
    14701 caaaacactg ttttgaggtt gcagatagag ctaacgtggt ttgaagagat tttcgaagag
    14761 tattaaagac agtgtgacta tgcttgtcaa tagataggaa atcaaaatga aggatggaaa
    14821 tctgagccaa acatcataag tgctataatg aacagaagta agaattgcac gataagtttt
    14881 ctaaaaccat aggaatcgtc ccctttcttc atcttcatta tagcacatat acatcagttg
    14941 tacaaataat atgatatttt tttattagtc ctcagacaag catattataa agcgtttcat
    15001 taccatttaa gttaatataa gctggatcaa aaccttccat tctacgaatc aaacctgcat
    15061 aatcatgctt atctgctaaa gcgatcccaa ttaatactgg gcctgttccc ttgctagctc
    15121 gtttgatata ctcaaaacgt gtgatatcat catttggccc caggatatca tttacaaact
    15181 cacgcaaagc tcctggacgt tgtgggaaat tgaccacaaa gtaatgtttg ataccatcat
    15241 aaatcaaggc acgctcttcc atttctggca tacggttgat atcattattt cctccagaaa
    15301 tgatacaaca aatggttttc cccttaatat attcagctaa aacctctaaa gaggcgatac
    15361 tagccgctcc agcaggttct gcgactatcc cttgcttaga gtaaaggtca atcaaggttt
    15421 cagaaatcaa tccctcatcg acacctacca aagttttaat atgttgacga gttgcttcat
    15481 aggtcaactg acctaccttt tgcacagcaa tcccatccgc aaatttatca atttccttga
    15541 gttttactgg acctccagcc tcaaaggcag ctttcatgga acgcgctcca ttcgcctcta
    15601 ctccgataac ctcaatctct ggacttgttt ccttgatata ggtagaaacc ccggcaatga
    15661 gaccgccacc accaacagga accaagacag catcaaaatc aatcgattct tttcgagctt
    15721 cttctaaaat ctcataagca actgttcctt gacctgcttg aacatgagca tcatcaaaag
    15781 gatcaataaa ggtacgattt tcagagactg taaattcttg agctgctttg gctgaggcat
    15841 caaaggtatc tccaactagt ttaatagtta caaaatcccc accaaaaaag cgaacctgac
    15901 caatcttttg ttgtggcgta gtaatgggca taaagatagt agcaggaatt ttcatttcat
    15961 tacaagtata ggctactccc tgcgcatgat ttcccgcaga agcgcagact accccacgtt
    16021 cacgttcttc cttgctgagc tgggaaatgg cataataggc accacgaatt ttaaaggagc
    16081 gaacacgctg ggcattttct tttttcaaat aaatcttagc accatacttc tccgataaat
    16141 aatgatcgta atccagtgga gtattcacaa ccacaccgtt caagaccttg tgagccttga
    16201 ttatatcttt tgaacttaac atataaaacc tctttttcta taaaaaacag tcactaatga
    16261 ctgtttttta gttattcatc tccgaaaact tcagtaacac tttcatggta tttatgagcc
    16321 atacgaatcg aaaactcttt aggaagttca taacgatttg tggaaatcca atcatataac
    16381 cattctacca aactttcttc actatcatta cgatggaaga taaaatagtt taaaatttct
    16441 ttatattcta catcattcaa atccaacgtg tctacaaatt gactacgagg aacaaaatta
    16501 gaatctaatt ttcttagttg atctgtcaat tctcccaatt gtaaatccaa ctgaaaaaat
    16561 agtttttgca gttgcctatc agtttcatca catcctgtct tgatagaaaa caaattggta
    16621 atattcatat gatttcaacc cctaaataag tacatctaat atcccacgaa tagctgaagc
    16681 taaagttttc gcagtccagt catttaaacc cgattctttc aatccagatg caataatttg
    16741 ctctatttga tataaaccgc catctatcgt ctcatttatt ctgcgaagag gaccttcaat
    16801 ataggataaa gcttctccga atctaacagc tgatttaggt cctaatgatt taccaactat
    16861 atttgttaat gttttttatt tctcaaaatc catttcgcta tataacgagc agatttagtc
    16921 gcaccagctc tctcaaacgg agaatgaatt tcaagacctt cttctctagc tatttgtgcc
    16981 aatttgaaga attgttcaaa ggtcatatgc aacctaccat cttcaaaatt agcatcatca
    17041 taaatgttaa ttacagcatt ttcatcatac gaccaatctg ctttattttg gtctgctagc
    17101 actattaccg acggtgtaat tacattatac aactagaacc aataataacg atgcagtttt
    17161 tctcataatc tttttcataa aatcatctcc agcgctatat atttctaatt agttatagat
    17221 tttgaatgca tcatcgtcgt ttttaccaac gaatggcatt gctttacgca attctgcacc
    17281 aactttttca atttcaaggt tagctgcttg ttcacggtaa gcagtcaatt ttggacgtcc
    17341 agctttatag tcatttacaa agtcatttgc aaatttacca ttttggatgt ctgccaagac
    17401 agccttcata ttttctttaa cttgttcagt gattacacgt ggacctgata catagtcacc
    17461 gtattcagca gtgtttgaaa tagattgacg cattttcttg aatccacctt cgtagatcaa
    17521 gtcaacgatc aatttcattt cgtgaagaac ttcaaagtaa gccaattctg gagcgtaacc
    17581 tgcttctgtc aagacttcga aacctgcttc gataagggca gtcaaaccac cacaaagtac
    17641 agcttgttca ccaaacaaat cttcttcagt ttcttctttg taagttgttt caagaagacc
    17701 tacacgagcc gctccaacac ctttacacca gtccatagca atgtttttag catttcctgt
    17761 tgcatcttgg tatactgcat aaagagctgg aacaccaaat ccttcttcgt aagtacgacg
    17821 taccaagtgt cctggtcctt taggagcaca catgaagaca tctacatccg caggaacttt
    17881 gataaattca aagtggatgt tgaaaccatg ggcaaatcca actgcgtttc cagcttccaa
    17941 gcttggagcg atttctgctt cgtacaattc ttgttgaatt tcgtctggcg ccaagatcat
    18001 gataacatca gccaacttag tagcttctgc tactgtgtaa gtatcaaatc catcttcttt
    18061 tgctttatca aaagatttac ctggacgtac accgataata acgtcacgac ctgaatcacg
    18121 caagttttga gcatgcgcat gcccttgtga accataaccg ataacggcga tttttttacc
    18181 gtcaagtgct gctactttaa catctttttc atattccatt tgaactgtca tagcttttct
    18241 ctcttttcta ttatttattg ccttttaggc tggtttaata aatttaagtt ggatttttaa
    18301 tcgcgggtaa atccagttgc acccgttcga gcaatattgc gaataccgta tgggcgaatg
    18361 actcgcaata gggcttcgct cttttctgca tttcccgtca tctgaatggt aatcgagctc
    18421 ggcgctacgt ctaccactgt tgcacggaaa ggttgaataa tcgctaaaat ctcagctctc
    18481 ttctcagctg gcgctgacat cttaaccaaa atcacctcgc gctccaaatg aggcttgtct
    18541 gtaatatctc gaatgcgaat cacatcaatc tgacgattga gctgtttgat gatttgctcc
    18601 acttcatcat gagaagcaac atcaataata atagtgatac gcgatacatt cggatcttct
    18661 gttgctccaa cagagatgct ttcaatatta acctgacgac gagataggac acctgtaaag
    18721 cgattgagga ctcctgaacg attttgtagt tttgctgtta acattctacg catggaactg
    18781 cacccccaac atctcatgat tactcttacc agccggtacc attggtaaca cctgttcctt
    18841 acgagaaata tctacctcaa ttagcatagg aacatcctca gtgatgactt caaggtcttg
    18901 agccaaggtc tcaggattgt caaacttata gtttttaata ccataagcct gcgccatcaa
    18961 ttggaaatca ggaagggtat caaagaccga ctctgatgtt ctgccttcat agaaggattc
    19021 ctgccactgg cgaaccattc cgagtgaatg attgttcagc ataaccacct tgattggcac
    19081 cttgtaaata ttcaaaatag ccaactcctg gttggtcatt tggaaaccac catccccaac
    19141 aaacaagact acttccttat ctgggttagc aattttagca ccgattgctg ctggaattcc
    19201 aaagcccatt gttcccaaac cacctgaagt cactaactga cgttcatttt ggtagggata
    19261 atactgagct gtccacattt ggtgttgacc aacgtctgtt accacaatgg catctccatt
    19321 cgtcaattca ccaattcgtt caataactgc ttgcggttga accacacgct ctttcttgtc
    19381 ataagaacga acacgattct tgtctttagt gactttctca atccactttt cagtattgtt
    19441 gtgaactgtt ggttctgcta gcaacatttg caaggccttc ttagcatctc caactacagg
    19501 aatgtctgca ctgataatct tgccaatctc agctgggtca atatcaatgt gggcaacctt
    19561 agcattctta gcgaaagtct taggattccc cgtcaaacgg tcatcgaaac gagaaccaat
    19621 actaatcata aagtccgctt ccgtcatggc aatatttgct gcgaatgacc cgtgcatgcc
    19681 tcccattcca agaaagagtg ggtgactcgt tgcaatcgtt ccttgtccca aaagactggt
    19741 taccactgga atttgatagc gttctgcaaa ttcatttagt tccgtagcag cctcagcata
    19801 actaattcca ccaccagcta acaagactgg ctttttagcc ttggacaatt gcttcaagat
    19861 tttcttgatt tgcatatcat tcggctcaag agtcggctga taacttggta aattcacttc
    19921 tggtgaataa atgaagtctg tttctaaagc agatatgtct tttggtaggt caattacaac
    19981 tggccctgga cggcctgtag ttgcgatatg gacagcttcc gtaatgatac gcggaatatc
    20041 agctgtctca cgaacttggt aattgtactt agtgattggc atggtaattc ccacgatgtc
    20101 tgcctcctga aaggcatcct tcccaatccc tgctcgcgcc acctgacctg taaagaccaa
    20161 aaggggaacg ctatcgctca tggcatccgc aatccctgta atggcatttg ttgctcctgg
    20221 tccactagtg acgacggcaa cacccaactt tccagttgat ttggcataac cttcagcttc
    20281 atgcaaacaa ccttgctcat ggcgccctag aatgtggcga atgcctttaa aattatatat
    20341 cgcatcataa aaaggcaaaa ccgcaccacc aggataacca aagatggtat caactcctaa
    20401 atcacgaagt gtttccaaaa ctaggtccga ccccgtctta ggagattcta aactgatttt
    20461 ctccattgtt cccctttctt tcctcttaaa aataacttgt tactatcata ccattttttc
    20521 aaaatttttc aagataaaag aagaaatttt ctgaattttc tattttaacg tttatttatg
    20581 aatgttattt ttgtttttat taaaaagata ccgatttcat ttactaaaaa agaaaaaaga
    20641 actgatttct cagtccttca ttaatcttat tccacactaa ataggtatgg gtaaacaggt
    20701 tgttgacctt ggtgaatctc gacttcaacg tcttcgaatt cttctacgat ttcttgagcg
    20761 atttcattgg caagttcttc gcttccgtct tcaccgacat agaaggttac gatttcactg
    20821 tcttcatcca acatatgttt caaggtttca gtcaatgttt ggtgcatatc agggtttgac
    20881 acaagaattt ttccatccac catacctaga ttatcgtttt catggatttc taagccatcg
    20941 atcgttgtat cacgcacggc tgttgtgacg cttccgctaa cgacatcgct aagagcagct
    21001 gtcatacgct cttgattttc ttcaatggac ttgcttggat caaaggcaag aagacttgtc
    21061 ataccttgag gaagagtgcg agcctctacc actactgctg gttgctccaa aacttctgcc
    21121 gcagattgag ctgccatgaa gatgttcttg ttgtttggca agaagatgat gttacgggca
    21181 ttgacctgtt caacagcctt gataaagtct tctgttgaag ggttcatggt ttgaccgcct
    21241 tcgataacat aatccacgcc ttgagaacag aagatatctg ctagaccttt accagctacc
    21301 acagcaatca aagcatactc tttttcttca gccgacttga taacttgagt agcttctttc
    21361 tcaacctgtg cttcgtgttg gttacgcata ttgtcaactt ttaccttgac caagctacca
    21421 tatttgagac cttcttgcat aacaagtcct ggatcttctg tatgaacatg gactttgaca
    21481 atttcatcat cgttaacaac gaggagagaa tccccaagct catccaagta gttacggaat
    21541 tcatcgtagt caaaatcttt agcataggtt ggaccttgct taagagctac catgatttca
    21601 gtacagtaac caaacgtgat gtcctcagtc gctacgtgac cagctacaga cttatgatgc
    21661 tctacattga tcatctcact catgttggca ggagtcgcta caaagtcctc agatgcaata
    21721 tattcgccag taagggctga aaggaaacct tcgtagatga agaccaatcc ttgaccacct
    21781 gagtccacaa cgccaacttc tttcaatact ggaagcatgt ctggtgtttt agctagagct
    21841 gttttagcac cttccaaggc tgcgcgcatg acttcaacag tatcatctgt ttgctcagct
    21901 tttttcttag caccgatagc agctccacga gaaactgtca aaatcgttcc ttcaacaggt
    21961 ttcatcactg ccttataggc aacttccaca cctgattgga aggccagagc caagtcttga
    22021 cctgttaact cgtctttatc cttgatagct tgggaaaatc cacggaaaag ctgagacgta
    22081 atcactcctg agttcccacg cgcacccatc aaaagccctt tggcaagaat gctcgctact
    22141 tctccaactg tagaagctgg cttgtctgca acttctttag caccattttc aatggtcatt
    22201 cccatatttg tcccagtatc tccatctgga actggaaaga cgtttaatga attgacatat
    22261 tcagcttgct tattcaagcg agttgatgca gcctgcacca tttcttgaaa taagctagta
    22321 gtaatttttg acacggttat tctcctacaa ctttgatatt ttgaatgtag acatttacag
    22381 tctgagcagt aattccaagc tggttttcca agctaaaggc aacacgctct tgaatgtttt
    22441 ttgacacttc actaatcttt gttccgtagc ttaacacggt atatacatca actgcaatac
    22501 tgccatcttc ggctgccttt acgacgacac ccttagaata attttcttta cctagcaggg
    22561 cttggaaatt atctttgagg gcatttttac tagccatacc gaccacacca aaaatctcag
    22621 ttgctgctcc acctacgacg gttgcaatca cttcatctgt tagttcgatt tgaccatctt
    22681 ttgtattaat ttttacagtc atccttttta cctcaactag ttgatactct attttatcat
    22741 atttcagccc aagtgtaaaa gcagaatgat gtatcagcgg atatttactc tatttttcaa
    22801 acgatgttat acccacaata aaagaaaaaa gaccctaagg tctcctttgc ttttattatt
    22861 aaacgcgttc aactttacct gatttcaaag cacgagctga agcccaaact tttttaggtt
    22921 taccatcgat aagaacagta actttttgaa ggtttggttt tacggcacgt tttgtttggt
    22981 tcatcgcgtg tgaacggttg tttcctgata cagtcttacg acctgtaaag taacatactt
    23041 tagccattgt gttttcctcc tattagatct aatatagcgg atgtgctagc accacatacc
    23101 gtactatgtt atcacacttt cttgtttttt gcaagggaat tggaagattt tttatttgtg
    23161 tcttaaatca ggtcttgcgt gatattttct gctctccaca tgccattgtt gattaacaga
    23221 acaccagaat taaaattatg tgtataaaaa tcatctctaa ctgcagctaa gggtatagcc
    23281 gtcaagtcca aatcccacag ctcatctatc gattttctta caacaatatc tgaatccaaa
    23341 tacagtacac gagactcgct tacatacttt ggaataaaat acctaaaaaa gccgtatatg
    23401 aaagtccctc aaaggggaga cgataacctt tcagaatact actgtcaatc taaacattca
    23461 caatctcact attcaaagtc tctagtcttt tttccatcaa ttggaaccat tctcgcggaa
    23521 ggtcatcatt aaaaacataa aacttaagat tataatgatg aacacaaaga gattttattg
    23581 ttgtttcaac tttatccata taagcattat ctgcacctaa gacaatcgct tttttctctt
    23641 ctttcacttt ttatctcatt tctttttatt cccatcatat gtttctacgt aaccattatt
    23701 ttcgcctatt cgttcgtaaa accataccag tggagatttt agatgaagtc ccattacggt
    23761 ttacaatttt tacattacga cacggagttt tacaaatcga tttcatttgc caaacgtagt
    23821 tagtaaggca gttagctagg ctacctactt tctgctacga cggaaaaatc cacaaatcaa
    23881 taccattcgt ggatttttcc tagtgaagcg tttaggtaag ccgccatagc ggttaccgat
    23941 tattagatta cgacacggag tttggcaaat cgattctatt tgccaaacgt agttagtaag
    24001 gcagttagct agttcaccaa atagcgacta gcgtccaaca atttggaact ttagttccaa
    24061 ttgttggtac tgagtcacat cttctcctct aactctacgt ctggatactt gtccgcaaac
    24121 cagcggaggg caaagtcatt ttcaaagaga aagactggtt ggtcaaaacg gtctttggct
    24181 aagatattgc gacttgacga catccgttca tccaagtcct caggcttaat ccaacgaacg
    24241 gtctttttac ccattggggt cataactact tccgcattgt actcgccttc catgcggtgt
    24301 ttaaagactt caaactggag ttgacctaca gcgcctagca tgtactcacc tgtttggtaa
    24361 ttcttataaa gctgaacggc tccttcttgc accaattgct caatcccctt gtggaaggat
    24421 ttttgcttca taacattctt agcagaaact ttcatgaaaa tttcaggagt aaaggttggc
    24481 aggggttcaa attcaaactt gttttttcca accgtcaagg tatccccaac ctgataagta
    24541 ccggtatcgt aaaccccgat aatatcacct gccacggcat tggtcacatt ctcacgactc
    24601 tccgccataa actgggtaac attagatagt ttggccccct taccagtacg aggaagattg
    24661 acactcatgc cacgctcaaa ttcgcctgat acgatacgaa caaaggcaat acggtcacgg
    24721 tgacgaggat ccatgttggc ttggatttta aagacaaagc ctgagaaatc cttgtcataa
    24781 ggatccacaa tttcaccgtc tgttttcttg tgaccatgtg gttctggagc aaacttgagg
    24841 aaggtttcaa ggaaggtctg cacaccaaag tttgtcaggg ctgaaccgaa aaagacaggc
    24901 gtcaattctc cagccagaat agcttcctct gaaaactcat tcccagcttc atttaaaagc
    24961 tcaatgtcat ccttgacttg ctcgtaaaaa ggattgctac caaaaagttt gtccccatct
    25021 tctaggctag caaaacgctc atccccttta taaagttcta aacgttggtt atagaggtca
    25081 tacaagccct caaaggcttt ccccatcccg ataggccagt tcatagggta gctagcaatg
    25141 cccaagattt cttccaattc ttgcaagaga tccaaaggct cacgaccgtc acggtccagc
    25201 ttgttcataa aggtaaaaac tggaatgcca cgatgtttca caacctcaaa caatttcttg
    25261 gtttgagcct cgatcccctt ggcagagtcc acgaccatga ccgcagcatc caccgccatc
    25321 aaggtacgat aggtatcttc tgagaagtcc tcgtgccctg gcgtgtctaa gatattcacg
    25381 cgcttgccgt cgtagtcaaa ttgcataaca gatgaagtaa cagaaatccc acgttgcttc
    25441 tcgatatcca tccagtcaga tttagcaaaa gtccctgttt tcttcccttt taccgtacca
    25501 gcctcacgaa tctcaccccc aaagtagagt aactgctcag tgatggttgt tttccccgcg
    25561 tccgggtggg agataatggc aaaggtacga cgtttcttaa tttcttcttg aatattcata
    25621 agttctcttt ctttgattct ctatttttct tgtttcaata gctgagaatg atttttacat
    25681 tggattttac cattcctttc aacactccat tatatcggat tttagcattt ttttcaattt
    25741 ctatttcttt tcacttcccc ctcccttatt tataggaaaa tatggtaaaa tagaacagac
    25801 taaaaatcat catttcacga aaggatgcaa gatgaaaatt acgcaagaag aggtaacaca
    25861 cgttgccaat ctttcaaaat taagattctc tgaagaagaa actgctgcct ttgcgaccac
    25921 cttgtctaag attgttgaca tggttgaatt gctgggcgaa gttgacacaa ctggtgtcgc
    25981 acctactacg actatggctg accgcaagac tgtactccgc cctgatgtgg tcgaagaagg
    26041 aacagaccgt gatcgcttgt ttaaaaacgt acctgaaaaa gacaactact atatcaaggt
    26101 gccagctatc ctagacgatg gaggagatgc ctaatgactt ttaacaataa aactattgaa
    26161 gagttgcaca atctccttgt ctctaaagaa atttctgcaa cagaattgac ccaagcaaca
    26221 cttgaaaata tcaagtctcg tgaggaagcc ctcaattcat ttgtcaccat cgctgaggag
    26281 caagctcttg ttcaagctaa agccattgat gaagctggaa ttgatgctga caatgtcctt
    26341 tcaggaattc cacttgctgt taaggataac atctctacag acggtattct cacaactgct
    26401 gcctcaaaaa tgctctacaa ctatgagcca atctttgatg cgacagctgt tgccaatgca
    26461 aaaaccaagg gcatgattgt cgttggaaag accaacatgg acgaatttgc tatgggtggt
    26521 tcaggtgaaa cttcacacta cggagcaact aaaaacgctt gggaccacag caaggttcct
    26581 ggtgggtcat caagtggttc tgccgcagct gtagcctcag gacaagttcg cttgtcactt
    26641 ggttctgata ctggtggttc catccgccaa cctgctgcct tcaacggaat cgttggtctc
    26701 aaaccaacct acggaacagt ttcacgtttc ggtctcattg cctttggtag ctcattagac
    26761 cagattggac cttttgctcc tactgttaag gaaaatgccc tcttgctcaa cgctattgcc
    26821 agcgaagatg ctaaagactc tacttctgct cctgtccgca tcgccgactt tacttcaaaa
    26881 atcggccaag acatcaaggg tatgaaaatc gctttgccta aggaatacct cggtgaagga
    26941 attaacccag aggttaagga aaccattcta aatgccgcta aacactttga aaaattgggt
    27001 gctattgtcg aagaagtcag ccttcctcac tctaaatacg gagttgccgt atactacatc
    27061 atcgcttcat cagaagcttc atcaaacttg caacgttttg acggtatccg ttacggctat
    27121 cgcgcagaag atgcgactaa tcttgatgaa atctatgtaa acagccgtag ccaaggtttt
    27181 ggtgaagaag tgaagcgccg tatcatgctg ggtactttca gtctttcatc aggttactac
    27241 gatgcctact acaagaaggc tggacaggtc cgtacactta tcattcaaga tttcgaaaaa
    27301 gtcttcgcgg attacgattt gattttgggt ccaactgctc caagtgttgc ctatgacttg
    27361 gattctctca accatgaccc agttgccatg tacttagccg acctattgac catacctgta
    27421 aacttagcag gtctccctgg aatttcgatt cctgctggat tctctcaagg tctacctgtc
    27481 ggtctgcaat tgattggtcc caagcactct gaggaaacca tttaccaagt tgctgctgcc
    27541 tttgaagcaa caacaggcta ccacaaacaa caacccgtga tttttggagg tgacaactaa
    27601 tgaactttga aacagtcatt ggacttgaag tccacgtaga gctcaacacc aattcaaaaa
    27661 tcttctcacc tacttctgcc cacttcggaa atgatcaaaa tgccaacact aacgtgattg
    27721 actggtcttt cccaggagtt ctgccagttc tcaataaagg ggttgtcgat gccggaatca
    27781 aggctgctct tgccctcaac atggacatcc acaaaaagat gcactttgac cgcaagaact
    27841 acttctatcc tgataacccc aaagcctacc aaatttctca atttgatgaa ccaatcggat
    27901 ataatggctg gattgaagtc gaactagaag acggtacgac caagaaaatc ggtatcgaac
    27961 gtgcccacct agaggaagac gctggtaaaa acacccatgg tacagatggc tactcttatg
    28021 ttgacctcaa ccgccaaggg gttcccttga ttgagattgt atctgaggca gatatgcgtt
    28081 ctcctgaaga agcctatgct tatctgacag ccctcaagga agttatccag tacgctggca
    28141 tttctgacgt taagatggag gaaggttcga tgcgtgtgga tgccaacatc tcccttcgtc
    28201 cttatggtca agagaaattc ggtaccaaga ctgaattgaa gaacctcaac tccttctcaa
    28261 acgttcgtaa aggtcttgaa tacgaagtcc aacgccaggc tgaaattctt cgctcaggtg
    28321 gtcaaatccg ccaagaaaca cgccgttacg atgaagcgaa taaagcaacc atcctcatgc
    28381 gtgtcaagga aggggctgct gactaccgct acttcccaga accagaccta cccctctttg
    28441 aaatttctga cgagtggatt gaggaaatgc ggactgagtt gccagagttt ccaaaagaac
    28501 gtcgtgcgcg ttatgtatct gaccttggtt tatcagacta cgatgctagt cagttgactg
    28561 ctaataaagt cacttctgac ttctttgaaa aagctgttgc cctaggtggt gatgccaaac
    28621 aagtctctaa ctggctccaa ggggaagtcg ctcagttctt gaatgctgaa ggtaaaacac
    28681 tggaacaaat cgaattgaca ccagaaaact tggttgaaat gattgccatc atcgaagacg
    28741 gtactatttc atctaagatt gccaagaaag tctttgtcca tctagctaaa aatggcggtg
    28801 gcgcgcgtga atacgtggaa aaagcaggta tggttcaaat ttcagatcca gctatcttga
    28861 tcccaatcat ccaccaagtc tttgccgata acgaagctgc tattgccgac ttcaagtcag
    28921 gcaaacgtaa cgccgacaaa gcctttacag gattccttat gaaggcaacc aaaggccaag
    28981 ccaacccaca agttgccctt aaactacttg cacaggaatt ggcgaagttg aaagaaaact
    29041 agacagaaca aaaccagccc taaggttggt tttttcttct ctaccaactc ccaataacta
    29101 ttttggcttt atttccagag tattttatgg taaaatgaag agtaataata tttattaaag
    29161 aggtaaaaac atgattgaag caagtaaatt aaaagctggt atgacctttg aaacagctga
    29221 cggcaaattg attcgcgttt tggaagctag tcaccacaag ccaggtaaag gaaacacgat
    29281 catgcgtatg aaattgcgtg atgtccgtac tggttctaca tttgacacaa gctaccgtcc
    29341 agaggaaaaa tttgaacaag ctattatcga gactgtccca gctcaatact tgtacaaaat
    29401 ggatgacaca gcatacttca tgaatacaga aacttatgac caatacgaaa tccctgtagt
    29461 caatgttgaa aacgaattgc tttacatcct tgaaaactct gatgtgaaaa tccaattcta
    29521 cggaactgaa gtgatcggtg tcaccgttcc tactactgtt gagttgacag ttgctgaaac
    29581 tcaaccatct atcaaaggtg ctactgttac aggttctggt aaaccagcaa cgatggaaac
    29641 tggacttgtc gtaaacgttc cagacttcat cgaagcagga caaaaactcg ttatcaacac
    29701 tgcagaagga acttacgttt ctcgtgccta atctctagaa agaggtcatt ctatgggaat
    29761 tgaagaacaa cttggcgaaa tcgttatcgc cccacgtgta cttgaaaaaa tcattgctat
    29821 cgctactgca aaggtagagg gtgttcactc tttttcaaac agatcagtgt ctgataccct
    29881 ttcaaaactt tcactcggcc atggcattta tcttaaaaac gtggacgaag aactcacagc
    29941 agatatctat ctctaccttg agtacggagt aaaagttcct aaggtagcgg ttgctatcca
    30001 gaaagctgtc aaagatgccg tccgtaatat ggctgatgta gaactcgctg ctatcaatat
    30061 tcacgttgca ggtatcgtcc cagataaaac accaaaacca gaattgaaag atctatttga
    30121 cgaggacttc ctcaatgact agtccactat tagaatctag acgccaactc cgtaaatgcg
    30181 cttttcaagc tctcatgagc cttgagttcg gtacggatgt cgaaactgct tgtcgtttcg
    30241 cctatactca tgatcgtgaa gatacggatg tacaacttcc agcctttttg atagacctcg
    30301 tttctggtgt tcaagctaaa aaggaagaac tagataagca aatcactcag catttaaaag
    30361 caggttggac cattgaacgc ttaacgctcg tggagagaaa cctccttcgc ttgggagtct
    30421 ttgaaatcac ttcatttgac actcctcagc tggttgctgt taatgaagct atcgagcttg
    30481 caaaggactt ctccgatcaa aaatctgccc gttttatcaa tggactgctc agccagtttg
    30541 taacagaaga acaataaaaa gaaaaagtgt ttgatatcta tcaaacactt tttctttgcc
    30601 tcccactatc taaaaaaatg ataatagata taattgtaaa caaaaatcca gataggtttt
    30661 gcatgattga gaaagttaaa aaaactatgg cagagaatcg ttaatctcag attgtcggta
    30721 gaacgataaa caagggcaaa aaagaaacca atcagactat aatataataa actaattgga
    30781 tctctgtgag atagtatcaa atgactaagc ccaaagataa ggcctgataa gatgacatct
    30841 aaataatact tggactaggg aaagaaggtg ttcataaaat accctctatc aagagtctcc
    30901 tcaaaaacag gaccgatgat tacaggcagg acaaaagata agatagtcga taaaaaggtt
    30961 ggttgtccat ttgaaagaac cacggtaaaa tactcatcat gaatattcct atgattaatc
    31021 aaatgagcat agcgtgccca aaaattaccg agaatctgat aaaccacata agttgcaaat
    31081 aaggagaaga caaatgacca gtcccagctc tttttctttt ttaacctcca aattaataga
    31141 aaaggaaact tcccactaat cccactgtta aaataagaga atagacatca actcctaacc
    31201 ctaaaatgat cgtcacatac aatccaattg tttgtggtaa ataggtagat agtaaaataa
    31261 taagcaaaaa tattccaaat tgtcttagtt tctttgtgtt tctcatcgta cttttttgaa
    31321 agattaccct gttcggaagc cgtacttcca agcatctata taagaattaa gtgccccttg
    31381 cctcatatag ggagcaaatt ctctataata taaccatcta ctatatccat cttcccaaac
    31441 agcaagacca cctgaagttt gctccaagtc ctcagttgaa ataactgtaa atgtttctgt
    31501 acctgtcatt gcaagtacct tcttaaaata gattgttgta ggctcacatt tatagtatat
    31561 ttcttttttt gtctatttta tagtctatct cctcaactgg caatttttcg acctgaattg
    31621 catttttcca taaaaaatga gacctttcta gtctcattta gtcattctta gtattttcta
    31681 aatcgttgat agcgttcttc cagcaactct tctagcggtt tttgtgaaag tctagccagc
    31741 tccgtttgga gttctttttt gacactctta atcagttctt tactagaaag tcctacttca
    31801 gaaatcacct tatccaccac atccatttct aacagttcat gcgaagtgat tttcatcagt
    31861 tctgctgctt ccatggcgcg agtaccgtcc ttccataaaa tggaagcaaa gccttctgga
    31921 ctgagaatgg catagataga attttccagc atccagacac ggtccgcgac agctagagcc
    31981 agagccccgc ctgaaccacc ttcaccgata ataatggcga taataggaac tttcaggtca
    32041 ctcatttcca tgagattgcg agcgatagct tccccttgac cacgttcttc cgctccgaca
    32101 ccaggataag cacctgctgt attaataaag gtcacaactg gacggccaaa tttctcagcc
    32161 tgtttcatca accgcagtgc ctttcggtag ccttctggat gtggttggcc aaaattccgt
    32221 ttgaggttgt cttgcaaact cttgcctttt tggataccaa ccactgttac agcttggtct
    32281 ccaagccaac caataccacc aacaactgca ccatcatcac gaaaagaacg gtcaccatgt
    32341 aattggataa attcatcaaa aatgcctgtc gcaaagtcca aggttgtcaa gcgactctgc
    32401 tcacgcgctt ctctgactat ttttgcaata ttcatctagg actccctcca tgcaatctga
    32461 ctaggctagc aatcgtatct ggtaagtctc ttcttttgac aatagcatcc acaaagccat
    32521 gttctaatag gaattctgcc ttttggaaat cctcaggcaa gctttcacga accgtatttt
    32581 caatcacacg acgcccagca aaaccaacca agctctgtgg ttcagccaga atgatatcgc
    32641 cttccatagc gaaagaagct gtcacaccac cagtcgttgg atctgtcaaa atggtcaggt
    32701 aaaagagacc agcatttgaa tgacgtttaa ccgccgcaga gatcttagcc atctgcatga
    32761 gactcatgat tccttcctgc atacgggctc caccagaggc tgtgaatagg acaactggca
    32821 atttttcgac agtcgcatac tcaaacaaac gagtgatttt ttcacctaca accgtaccca
    32881 tagaagccat gataaagtta gaatccataa tcccaagagc cacagtctga cctttaataa
    32941 gagcagttcc tgtcacaacg gcttcatgca gacctgtttt ttcacgcata gatgccagtt
    33001 tcttttggta accagggaaa tgcaagggat ccttgctttc aatccctgta aacaattctt
    33061 tgaaggttcc catatcaatc gtcaaagcca agcgttcttg ggcagaaata cgaaaggtat
    33121 agctacagtg cggacagata cgttcacttc ccagatcctt ctgatagatg gtatgcttac
    33181 agcctggaca ctgggaaaat aattcatctg gaacctctgg cttagcttga ggtttttccc
    33241 taaccgaacg attgggattg attcgaatat acttatcttt tttactaaat agagccattg
    33301 attccccttt tcggtttaaa ctcttaaagt cattttattc tttttcttga tatttaggta
    33361 agaaggtttc catcaagaag gaagtatcat aatccccagc aatgacattg cgatctgaaa
    33421 tgaggtcaag ctggaaatct gcattggtct gcactccttc aatctctaat tcatagaggg
    33481 cacgttgcat tttcatcaag gcgtcaaaac gattttcgcc gtgtactatg attttggcaa
    33541 tcatactatc ataataaggc ggaatggtat aacctggata aactgctgaa tccacgcgca
    33601 agccaactcc accacttggc agatagagat tagtaatctt acctggactt ggagcaaagt
    33661 taaaggctgg gttttctgca ttgatacgac actcgatggc atgaccgcgt aggacaatat
    33721 cttcttgctt aacagacaaa ggctgacctg ccgcaatgca aatctgttcc ttaacgatat
    33781 caacacctga aacaaactct gttactggat gttctacctg aacacgagta ttcatctcca
    33841 tgaaatagaa attgctactt gcttcatcaa gaagaaattc aatggttcct gcattctcat
    33901 agccaacaaa ctctgccgct cgaacagcag cagcacctat ttcatgacgc agcgtttttc
    33961 cgattgcaat cgagggactt tcttccaaaa ccttttggtt attcctttga agagaacaat
    34021 cccgctcacc caagtgaatc acatgtccat gctcatcacc taggatttga acctcaatgt
    34081 gccgagctgg atagataacc cgttctatgt acatggcacc attgccataa ttggccttgg
    34141 cctcactaga ggcagtttca aaggcagaaa cgaggtcatc tggtttttca accttacgaa
    34201 tccctttacc acctccacct gctgaagcct tgagcataac aggatagcca attttttcag
    34261 caacaatcaa agcttcttca gagttatgca cttctccatc tgaacctggt ataacaggca
    34321 cacctgcttt aatcatctga gcacgcgcat tgatcttatc ccccatcata tccataacat
    34381 gaccagatgg accgataaac ttgataccta cttcttcaca catggtcgca aatttggaat
    34441 tttcactgag aaatccaaaa ccagggtgaa tagcttctgc ctcagtcaag actgcagctg
    34501 atagaactgc attaatattg agataagact ctgttgcctt gccaggacca atacaaactg
    34561 cttcatctgc caaaagcgta tgaagagctt ccttatcagc agttgaataa accgctaccg
    34621 tcgcaatccc caattcacgt gccgcacgga taatacgaac cgcaatttca ccacgattgg
    34681 caattaaaat ttttcgaaac atggagaacc tccttagttc ccaattgcaa aagtaaggat
    34741 accactggct gcaagcttgc catccacttc agcctttgct tcaaccacag ctatggtgcc
    34801 acgacgtttt acaaaagtcg ctgtcataac caattggtcg cctggtacaa cttgcttctt
    34861 gaacttaacc ttgtccatac cagcgtaaaa gaccagtttt cctttatttt caggttttga
    34921 taactccaac acaccagcag tttgcgccaa ggcttccata atcagaacac ctggcataac
    34981 tgggtattga ggaaagtggc cgttaaagaa aggttcgtta atggtcacat ttttgatagc
    35041 aacaatggta tcctcgctca cttccaagac acggtccact agaagcatag gataacggtg
    35101 gggaagagct tctttgattc cttgaatatc gatcatttga tacgtaccaa tcctttacca
    35161 aactcaacca tttcttcgtt agagacgaga atttccgtta ccacaccatc cttaggagct
    35221 gggatttcat tcatgacttt catggcttcg ataattacca atgtttgacc ttttttgaca
    35281 ctatcaccaa ctgtaacaaa ggcaggttta tctggtccag cagccaagta aaccactcca
    35341 acaagtggac tctctacaag atttccctca gtagccacac ttgcttcagc tggagctgga
    35401 acttcttctg ctacagtctc tgctggagca gatgtaggag ctactggact cggtgttgct
    35461 agaacgggtg ctggagcgac ttgagttgca acttcaggca caggtcttgc ttcattcttg
    35521 ctaaactgca actcatccgt cccattttta taagaaaatt ctctcaaact tgactggtca
    35581 aattgagtca tcaagtcttt aatatcgttt aaattcatac ttatctattc tcccaacgtt
    35641 tgaaagcaag aactgcattg tggcctccaa aaccaaaagt atttgaaata gcgtatggaa
    35701 tttctttctc caagccttgt ccataaacga cattagcttc gatataatct gatacttcac
    35761 ttgtcccagc tgtcattggt acaaagttat ggcgcatagc ttcgatggtg acgatagctt
    35821 ctactgcacc cgcagccccc agcaaatgtc ctgtaaaaga cttggttgat gatacaggta
    35881 cttccttacc aagaacagct acgatagctc cactttctcc tttttcattg gcaggagttg
    35941 acgttccgtg agcattgaca taggctactt gctctggaga aatctcagct tcttccaagg
    36001 ctagtttgat ggccttgata gctccctgac cttctggatg tggagaagtc atgtggtagg
    36061 catcacaagt atttccgtaa ccaaccactt cagccaggat agtagctcca cgtttttcag
    36121 cgtgttcaag actttctaaa accaacatcc ctgaaccttc acccataaca aacccattgc
    36181 gatccttatc aaatgggatc gaagcacgag ttggatcctc tgtagtagag agagctgtta
    36241 aggcttggaa accagcgatg gcaaaaggtg tgatagaagc ttctgttcct cccaccaaca
    36301 tcacatcttg gaaaccaaac ttaatggagc ggaaggcatc cccaatcgca tcatttgatg
    36361 aagagcaggc agtattgata gatttacaaa caccgtttgc accaaaacgc atggctacat
    36421 tcccagaagc catatttggt aaagcttttg gaagagtcat tggtttgaca cgtttgggtc
    36481 ctttttcatg aaggcgaagt acctgatctt caatttcctt gattccacca ataccagatg
    36541 caacgataac accaaaacga tccctattaa gagcctctac atcaagattg gcatgattta
    36601 cagcctcttg ggctgcatac aaggcatata aagaatagtt atcaaaacgg ttggtatctt
    36661 tttttacaaa gtatttatcg aacggaaaat cttggatttc tgccgcatta tgcacatcaa
    36721 agtcactatg atcaaatttt gtaatgccac caatgccgat tttcccagtt gctaaactat
    36781 tccaaaattc ttctggtgta tttccgattg gagatgttac tccataacct gttaccacta
    36841 ctcgatttag tttcattctt ttcacctcta gctttcgcta catacttaag ccaccatcaa
    36901 tggcaatcac ttgaccagtt agataatctt ggcctgctaa aaatactgtc aaatctgcaa
    36961 cctgctctgc ctgcccaaat tctttcatcg gaatctgagc tagtgtagct tccttaatct
    37021 tatctgataa gatagcggtc atatcagact caatcattcc tggagcaatc acattgactc
    37081 gtatattccg actagcgacc tcgcgtgcca cagacttggt aaagccaatc aagccagcct
    37141 tagaagcagc atagttagct tgaccaatat tccccatcaa accaacaaca ctagacatat
    37201 taatgatagc accttctctg gctttcatca tcggtttcaa gactgattgt gtcatattaa
    37261 aggcaccagt cagattgacc ttgagcactt tttcaaaatc tgcttctgtc atcttgagca
    37321 taagagtatc ttgggtaatc cctgcattgt tgaccaaaac atctactgaa cccagttctg
    37381 caatagcttg atcaatcata cgcttagcgt ctgcaaaatc tgatacatct cctgaaatgg
    37441 gaaccacctt gataccatag tttgaaaact cagcgagcaa ttcttctgag attgccccac
    37501 gactgtttaa gacaatgttg gctcctgctt gagcaaactt gtgggcgatg gcaagaccaa
    37561 ttccacgact cgaacctgta ataaagatat ttttatgttc tagtttcatt tttttccttt
    37621 caaaacttct acttatttta gtctattttt ctaaaagtgc tactaaactc gcttgatctt
    37681 ccacatgagc taagtgagca gtttgatcaa tttttttaac aaaacctgac aagactttcc
    37741 ccggtccaat ctcgataaag ttgcttatgc ctgcttcttg catgacccca atactttcat
    37801 agaaacgaac gggttccttg acctgacgcg tcaagagctg agcaatgtcc tctttttgca
    37861 tcacagcagc ttctgtattg ccgactaggg gacaagtaaa atctgaaaaa cttacctgag
    37921 ctagagtttc agctagtttc tggctagctg gctcaaggag agcggtgtga aagggacctg
    37981 acaccttaag aggaatcaag cgtttggcac ctgcttcttg caaaagttca accgctcgat
    38041 caactgcaac cacttctcca gcaatgacga tttgtgcagg tgtgttatag ttggctggag
    38101 taaccactcc aagttcagaa gctttttgac aggcttcttc aatgacctct actggcgtat
    38161 tgagaactgc taccatcttg ccagagtcag caggagtcgc ttcttccata taggctccac
    38221 gcttagctac caaggcaacc gcatcttcaa aatccaaggc gccgcttgcc accaaggcag
    38281 agtattctcc aagagacaaa ccagcaacca tatcaggctg atagcccttt tcttgcaata
    38341 aacggtagat agcaaccgaa gtcgctagaa tggctggttg cgtatagcgg gtctgattga
    38401 gtttgtcctc ttccgtatcg atgagataac gcaaatcata accgagcacc tgactcgctc
    38461 gatcaatcgt ttctttaaca atcggatact gatcatagaa atcccgtccc atccctagat
    38521 actgggcacc ttgaccagca aataaaaagg ctgttttagt catttcttac aactcctgtc
    38581 cagcgagagg cttcttcttg aattttctta gcggctccgt aatacaaatc ttttaggatt
    38641 tcttcagctg tttcttcttt agaaacaagc cctgcgattt gacctgccat gacagagcca
    38701 ccatccacat caccgtgaac aactgctttg gctagggcac ctgctcccat ttgttcaaag
    38761 atttctaaat caggatcttc ctgcttaaag gcatcttttt cagccagttc aaaatctcta
    38821 gtcaactgat ttttaatagc acgaacagca tgaccaaagt gctgagctga aatcgtagtg
    38881 tcaatatccc ttgcttttaa aattttctcc ttgtagtttg gatgggcatt cgactctttt
    38941 gcaactacaa accgtgtccc cacctgtaca gcctctgcac ctagcataaa gccagccgca
    39001 gcaccttcac catccgcaat tcctcctgca gcaataacag gaatagatat agctgtggct
    39061 acctgtcgca ccaaggtcat ggttgttaat ttgccgatat gccccccagc ttccattcct
    39121 tctgcaataa cagcgtctgc accgattttt tccatgcgtt tagctaaagc gacactagga
    39181 acaacaggaa taacgattat cccagcttca tggaaacgtt ccatatactt gcttggattt
    39241 cctgctcctg ttgtgacaac tttaacacct tcttcaataa cgagatccac gatatcttcc
    39301 acaaagggag ataagagcat gatgttgacc ccaaagggtt tatcagtcaa tgatttgatt
    39361 ttatcaatat tggccttgac aacttctttc ggggcatttc ccccaccgat aattcctaat
    39421 cctccagcct tggaaacagc ccctgccaaa tcaccatcag caacccaggc catccctcct
    39481 tggaaaatag gatagtcaat cttcaataat tctgtaatac gcgttttcat agtgcctcca
    39541 accttccttg cttacgtaat agttcgattt caccataatt tgacagtcaa actattacct
    39601 aaacaagagg gagtgggttt ctccctactc cttctactaa tattctgctt attttgcttg
    39661 ctcttcaacg taagcaacca agtcaccaac tgttttcaag tcattttctg cttcgatttg
    39721 gatatcaaaa gcatcttcga tttctgagat tacttggaac aagtccaatg aatctgcgtc
    39781 caaatcatca aaagttgatt caagtgttac ttctgatgcg tcttttccaa gttcttcaac
    39841 gataatttct tgtacttttt caaatactgc catgatagga ctcctttaaa ataaatagtt
    39901 tttttataac aatgtgttca ccacatgatt acctaaattg taagaatgag cgtgccccag
    39961 gtcaagcctc caccgaagcc tgatagaaga acagtctggc taccatctaa agggatgaga
    40021 ccttgttcta cacactctga aagtaaaatc gggatactgg ctgcactggt attgccatat
    40081 tccatcatat tggctggaag tttggctcgg tcaacaccaa tttttctagc catcttatcc
    40141 aaaatacggt cattggcttg atgaagtagc agataatcca agtctgtcac ctctatagga
    40201 gattcatcaa tagtctgctt gatagacttg gctacatctc gaatggcaaa atcaaagact
    40261 gtgcgtccat ccatcttcaa aaacgaatct gcactttctt gatctgaaaa tggagaatgc
    40321 aaacctgaat gcccataagt taaacactcg ctgcgacttc catcgctatt gagactctca
    40381 gctaagaaat gctcttgctc gctagcttct aacaagacac caccagcacc atctccaaac
    40441 aacacagctg ttgatcgatc cgaccaatcg actgccttag agagggtttc actaccaatc
    40501 accaagcctt tttgaaagcg accagaagcg ataaactttt cagcagttga aagagcaaat
    40561 acaaatccac tgcaagccgc ggttaagtca aaagcaaagg ctttattagc gccaatatta
    40621 gcttgaacac gagcagctgt agagggcatc atcgaatctg gagtaatggt agctaggatg
    40681 ataaaatcca gttcttttcc tgttattcca gcttttgcca tcagtttctt agcaacctct
    40741 gtagccaaat cactggtaga ttctgttctt gaaatatgcc tttgtcgtat tcccgttcga
    40801 cttgaaatcc actcatcatt ggtatccata atctgagcca agtcgtgatt tgtaaccact
    40861 tgctctggca cataatgagc aacctgactt atttttgcaa aagccattat ttcaaatcct
    40921 ccaaaaattg gtaaagatta gtcaagcctt tacccatgac agcaatttct tcctcgctca
    40981 tgccatcaat aattttttct accatggcct tgtggaagcg tttatgcagt ctatgaatca
    41041 agcgaccctt ctttgtcaaa tgcagatgca ccacacgacg atcctgttct gaccgaactc
    41101 gctcaatgta gcccttacgc tctaaattgt tcaaacttgt cgtaacagtt ccaagagtta
    41161 ccatcaactc ttttgacact tgacttggag tcacatctgg agccttaccg atgacatcaa
    41221 tcgtatgcat ttctttgatg gagatatcct taaaacgact acctctcaag ttcacttcct
    41281 caattacaag gacattgtta aatatagatg ttaaatattc attgattcgt tggtagtcca
    41341 tttttcatat ccctccttct tcaaaaaaac ttttacaatc aaaacatttg acagaaagaa
    41401 ttatatcaaa attcaaagaa tgatgcaagt attttttatt ttcctgtaaa tttaggtctt
    41461 cttctctccg aatgagctcg cactccttct ttgaaatcct ctgtttgagc taaggatttc
    41521 tgtaggttca gttctaaagt agcgtaaccc tgccagtctt taaattggct ctcccaaacc
    41581 aacttcttaa tggcagcata ggaattactt gaagcacgtc ttaatttttt aagaagctgt
    41641 tctctcgtct tttcaagttt ttcagcttca gaaacgcggt aaaccaaccc ccattctagg
    41701 gctttttctg ccgttaaagc ctctcctgtc atagctaatt gagcagcacg cgtcacaccg
    41761 atgctgcggc tcaagagatg aattccccct gcatctggag ccaaaccaac accaacaaag
    41821 gcttggataa acttagcctt atccgtcgcc aaacagaaat ctgccgcaac agccatattc
    41881 gctgcggcac ctgcaacagc accgtcaact tccattaaaa caggtttggc tatttgcttg
    41941 attttataag aaatcgtatt gaccaactct gcgatttttg tcaatgatgg aatatcatcc
    42001 tcatccactg ctcgcttcat ctctaccaaa tctcccccaa ctgagaagac ctttccattg
    42061 gcattgatta agataaaatg cacagctgga ttttcttctg ccaaagtcag agcttctaaa
    42121 atctcctcac acatgggaat atgaaaacca tttgcgacct caggacggtt caaggtaagg
    42181 attgccaagt cctcttcaag ctgataaata atgtgttcca tcagggctcc tttttatttt
    42241 ataagcagtt tatactcttc gaaaatctct tcaaaccgcg tcagcttcca tctgcaacct
    42301 caaagcagtg ctttgagcag tctgcggcta gcttcctagt ttgctctttg attttcattg
    42361 agtgttaaat tatttcactg tcaaagtata tcttttttag gagcagaaag caagaaaaaa
    42421 tcgactgaaa gtgtctcagc cgataagaaa ggctcgcttt aggctagaaa gcatcttatt
    42481 ttcttcattg aaaaaagcct ttctttctgc tataatcgta taaaatactt actttaggag
    42541 ttcttatgaa agttgttaaa tttggaggta gttctcttgc ctctgctagt caattagaaa
    42601 aagttttaaa catcgtcaaa agcgattcag agcgtcgttt tgtagtcgtt tctgcgcctg
    42661 gtaaacgcaa tgctgaagat actaaggtta cggatgccct gattaaatac taccgcgact
    42721 atgttgcggg taacgatatt agcaagagcc aaagctggat tatcgaccgc tatgctgcta
    42781 tggttagtga attgggacta aaaccagctg tgctagaaaa aatttctaaa agcattcacg
    42841 ccttggccac tcttcctatt gaagaaaatg aatttctcta cgatactttc ctagcagccg
    42901 gtgaaaataa caatgccaaa ttgattgctg cctactttaa ccaaaatggt atcgatgcac
    42961 gctatatgca ccctagagaa gctgggattg tggtcacaag tgaacctggt cacgctcgca
    43021 tcattccatc aagttatgac aagattgaag aattgacaaa caccaatgaa gtccttgtca
    43081 ttcctggttt ctttggtgtc actaaggaaa atcaaatctg tactttctca cgtggaggtt
    43141 ctgatattac aggttctatc attgctgctg gtgtcaaagc tgacctctat gaaaacttta
    43201 cggacgttga tggtatcttt gcagcccacc ctggtattat ccaccaacca cactcgattc
    43261 ctgagttgac ctaccgtgaa atgcgcgagt tggcctatgc aggcttctca gtccttcatg
    43321 acgaggctct tcttcctgcc taccgtggaa aaattcctct ggttatcaag aataccaaca
    43381 accctgacca tccaggtact cgtatcgttc taaaacacag taatgatgaa tttccagttg
    43441 tgggaattgc tggtgactca ggctttgtca gcattaacat gtcgaaatat ctcatgaacc
    43501 gtgaggttgg atttggccgc aaggttctgc aaatcctgga agaacttaac atcggttggg
    43561 aacatatgcc aacaggtatc gacgatcttt ctatcattct ccgttctcgc gaactaactc
    43621 ccatcaagga agaagaaatc ctgcgtcagt tggttcaaaa gactaaagta gatcatgcag
    43681 aaatcgaaca cgacctttct atcattatga ttgttggtga aaaaatgaag agccacatcg
    43741 gagtgactgc tactgcgaca cgcgctctat ctgaaaacaa aatcaacatc cagatgatgt
    43801 ctcaaggttc tagtgaagtt tctattatgt ttgttgtcaa taaagaccaa gagaaagcag
    43861 caattaaagc cctctacaat gccttttttg gaaaaagtaa ggaagactaa gcatggccca
    43921 atcacttaac aaaacagtac tcctcagtac gacaggcact tcctacctct ctatcgctgg
    43981 gaaagttggg aaattccttg tcggagatca ggctttggaa ttttacccag atgtcaatgt
    44041 cgaacaattt atccagattc cttggagcca tatcaatcag attggagcca atgtcactgg
    44101 acgcaaaatc agccgccact tcgaagtctt tacagacaaa ggaaaattcc tctttgcttc
    44161 aaaagactca ggtgccattc tcaaaattgc acgcgaaaaa ctaggcaatg acaaggtcgt
    44221 caaacttccg accctgattc agacaattag ccaaaaattt aaaaatctat ttgcaaaaaa
    44281 gtagaaactt tagtataatc atagcaacgg ataagtaggt tatcttcttc tttcagaaag
    44341 tctgcgggta ctgcgagcag ataggaggga taggtgaaat tctaccgtta gtgacaatta
    44401 catacacaat caagtgcaca cctgtgaagt tggatggaac cgtggccctg ccactccaac
    44461 gttttgtcag ggtgcttttt tcataaagga gttcttatgt tagatatcaa acgtattcgt
    44521 acagattttg aagctgtcgc agaaaaatta gctacacgtg gtgtagatgc tgctgtcttg
    44581 aatgaaatga aagaaatcga tgctaaacgt cgtaacatct tggtcaaggt tgaaactctc
    44641 aaagcagaac gtaacacagt ttctgctgag attgcccaag ctaagcgcaa caaggaaaat
    44701 acagatgaca agattgctgc catgcaaaat ctatctgctg aggttaaagc cttggatgct
    44761 gaattggcag aaatcgatgc taaattgaca gaatttacaa cgactcttcc aaatatccca
    44821 gctgacagcg ttcctgttgg ggctgacgaa gacgacaatg tggaagttcg ccgttggggt
    44881 actccacgcg agtttgactt cgaacctaaa gctcactggg atctcggtga agaccttggt
    44941 atccttgact gggaacgcgg tggtaaggta acaggcgctc gcttcctctt ctataaaggc
    45001 ctcggtgctc gtttggaacg tgctatctac aactttatgt tggatgaaca tggaaaagag
    45061 ggctatactg aagtcatcac accttacata gtcaaccatg attctatgtt tggtactggt
    45121 cagtatccaa aatttaagga agatactttt gaactcagcg ataccaactt tgtcttgatt
    45181 ccaactgctg aagttcctct gacaaactac taccgtgatg aaatcttaga cggcaaagat
    45241 cttccaatct acttcactgc tatgagtccg tcattccgtt ctgaggctgg ttctgccggt
    45301 cgtgatacgc gtggcttgat ccgtttgcac caattccaca aggttgaaat ggtcaaattt
    45361 gccaaacctg aagaatctta cgaagaattg gaaaaaatga cagccaacgc tgaaaacatt
    45421 cttcaaaaac tcaaccttcc ataccgtgtc gttgctctct ctactggaga tatgggcttc
    45481 tcagctacga agacttacga cttggaagtg tggattccag cacaaaacaa ttaccgtgaa
    45541 atctcaagct gttcaaacac agaagatttc caagcccgtc gtgcccaaat ccgttaccgt
    45601 gatgaagcag atggcaaggt gaaactcctt cataccttga acggttctgg acttgcagtt
    45661 ggacgtacag tggctgcaat tcttgaaaat taccaaaatg aagatggttc tgtgaccatc
    45721 ccagaagcac ttcgtccata catgggtgga gctgaagtca tcaaaccata aaaaaataag
    45781 gtttagctat ttctagctag accttttttc gtaaccaaat cagataagca cctagtacaa
    45841 agaataaaat agttaggcat ataatggttt cagtcaatac caggtaatcc agaaatggaa
    45901 gtttcaaaat tccctgagcc atcttgagcg aggtcgctgt gataatggtt gggaaggtga
    45961 gggctgagaa ggctggttga aaaccttgtt ttaaaatgtt gggcagacga gttaaaacaa
    46021 agaaaaagaa ggattgagaa gccaaaatca tgacaatcaa gacccaagtc ggcaggctgg
    46081 ttcctcctac tcgaactaga gaagccaaga gtagagagaa aggagcacag tagattcctt
    46141 cttgtccaag caaggctagt gggagtggat gtttctttaa atcgctataa ataaggggat
    46201 agagatagaa ggtcaagaga aaaccaaaac tcaaggtcgc ataggcaatt tcgataatac
    46261 ctaccagagg ataggtcaag gcagccactg ctatccccac atagagaacc gtccaacttg
    46321 gagtggcatg aaccctccgc cctggacaag caaacttgat ggtaaaacca gcaatcaagg
    46381 tcaaatccaa gagaaatgaa aaccaccaaa tcccttgggc taccaaagga agataagaga
    46441 atacgcgaaa gacataggtc gataaaatca tcccagccat aggaaaggtt gccattcctg
    46501 acaaaagagg gggcttggtc aattcttgct tggtttcttt ccaattaaag agatgcagaa
    46561 ttagaaagta aatccataaa accaaaccag tcagactaaa aagatgggat agaaccggca
    46621 acgtatctaa aataagattt ccagctcctg ccaaacctag caaacaacct gaaaatacta
    46681 aggggagttt tttcatccta acctccaata atcatgttag tttcagtata acataaaagc
    46741 gcttaaatga ggatttaaaa aacgaagtcc gcttatttca gacttcattt tactcagata
    46801 tgaattaggc ataaggttgc aattctggat taattggtgt attagctaag ttgttggcat
    46861 agttacagag gattgctagg ctgacaccaa aaaccacatc caaggcattt tgttgagtgt
    46921 agccagcttc taaaaactca gacaaggctt catctcctac acgacccttg gtattgataa
    46981 ctgccaaggt aaacttagct agggtatcca atttaggatc tgtttcaatt ggagtacgat
    47041 tgcgaagagc ttgaatcaag tcatcattca tctggatttg tttgatggaa aaggctgtgt
    47101 gacctgcgac acagaaggca caaccattgg tcacggctgc cgtgatttgc accacttcac
    47161 gctcaacggg tgtcaggctg ttgcgacggt ggatagatga gacaatttgg taggcttcta
    47221 aaacagtcgg ggcattggcc aagagaccga ttaggttggg aatatagcca ttgttgtctt
    47281 tttctactgt ttcaagaatt tctttcactt ctgctggtgc tgactctact gtatggatag
    47341 taaatgttgt cataagatac ctcttttctt attattgaca ctaatattat tggaaaatct
    47401 tataaaatcc tgattcctaa gtttatctaa gataaagctt tattctctca taagattttc
    47461 gttgttatat tagtttatca cacttccaat cacttgcata atatatatta tatatcaggc
    47521 tgataaaaat tatttatagg caaaaaaatc acacgagctg tgtgattcca ttatttgtca
    47581 aaatactttt tagtttcagc aataacgact ggcgacaaga ccaagagggc aatcaagttt
    47641 ggcagagcca tcaaggcgtt aacgatatct gcgataatcc agaccatatc caactcgata
    47701 aatcctccta acaagaccat gagcacaaaa accacacggt agagccagat aaagcgaacc
    47761 ccaaagagga actcaaaaca gcgttctccg taatagttcc aacctagaat cgttgtaaag
    47821 gcaaaaagta caaggaagat ggtcaagagg gcaggcccaa agtgtgaaaa gactgttgag
    47881 aaagctgact gagtcaaggc aaccccattc aagtcaccac tccaaactcc agttaccaag
    47941 atggtcaaac cagttagagt acaaatgatg agggtatcaa taaaggttcc tgtcatggaa
    48001 atcaaacctt gctctactgg ttcatttgtc ttggcagctg cagctgcaat aggagcagaa
    48061 cccagaccag attcgtttga gaacacacca cgcgccacac cattttgaat agccatccga
    48121 acgctagcac cagcaaatcc acctaccgca gcaaggggac taaaagctga ggtaaagact
    48181 aaagcgattg tgccagggat ttttccgata ttaaagaaaa taactgtaag agttcctaag
    48241 atataaatga gggccataaa aggaacaaca gtagttgaaa ccttagaaat agacttgagt
    48301 ccaccaaaga ctgcaatcgc tacaaagaca gacaagacga gagctgtgat ggctggcgaa
    48361 atcgtcgttg tattttggat agattctgta atcgagttga cttgggtgaa ggttccgatt
    48421 cccaagagag caaccaatac tcctgctact gcaaacaaaa cagcaagtgg tcgccacttt
    48481 tctcccatcc ctagaaggat atagtgcatg ggacctcccg ctactgcacc atggtcgtcc
    48541 ttggtgcggt atttgatggc caagagtcct tccgcatact tggtagccat tccaaagaaa
    48601 gccgccatcc acatccaaaa tagagctcct ggtccaccaa ccttgatagc cgtcgcaact
    48661 cctatgatat ttcctgttcc aacagttgat gccaaggctg tacacagagc tgcaaaactg
    48721 gatacatcac catgtccctt atcctggata aaaataagct gaaaggcctt gggcagacgc
    48781 aaaacctgca agagtcctag ccgcatggtt aggtaaatcc ctgttccgac caataaaatc
    48841 aagaggggcg gtccccaagc aaaagcatcg attgatttaa gcaattctaa catttccttc
    48901 tcctatcgtt tcaaccccaa aagaaagagc acatgcaaga cacatgtact ctggaatgct
    48961 tagataaatg ctaaaaagcg gtctatccta gctctgtcct tttacctgag agtttgagca
    49021 gttgcctgcc ttgccccttc ggtgccttta cggtctctcc agagttccgt ccatttacag
    49081 tcatggaaaa tcaaacgatt ccccacttct attaaacttc attcggtgtt ggtatttaat
    49141 tgattctaat tttacaaaaa atgttggctt ttgtcaatgt gtttattagt aaaaattagt
    49201 tcaacagttt ttactttata aagtccagaa tactgctatc ctttaaaagt gacaatagtc
    49261 gcaccactgc ctccagcatt ttgtggggca tagccgaaac tcttgacatg tttgtttctt
    49321 tgcaagtatt tggtaactcc ttcacggatg actcctgttc cgataccatg gatgatatca
    49381 acttgagcca tattgttaag caaggcttgg tcgatgaagg catctagctc attcatggct
    49441 tcttcatagc gcttgcctcg aagatccagt ctagcttgag gtcctcgccc agaagttcgt
    49501 ttcacaacat tgacctgttt cttcttgact ggtttttctt gctgggcttg aacaagatca
    49561 aactctttct cttccaaggt catcttaatc aagccaactt gggcttccca gcgaccgtcc
    49621 ttgagttgac tggtcaaggt accacgctga ccataactga gaaccacgat atcatctccc
    49681 acctttggag ctcgtttttt cttggccttt tgaaggacct tatttttaga caagtccact
    49741 ttttcaggag ccaatttttt caacttggcc ttggcttcaa tgatttcgtg gggcttgagt
    49801 tgggatttac tgtggagatt tttgagaatc tggtcacttt cacttagggc catatccaca
    49861 atctcagcag cctgttcacg cgccttgtta agctcgattt ccttttcacg attaagctcg
    49921 ttgtagagtt tttttagcgc acggttcatc ttgagatttt cttgctccac ctcacggata
    49981 ttgtccaaac gtttgcggct ttccagcgtc tgctcttcta attgctcaat gatacgattg
    50041 acgtcattgt cctgatcgat ctgctgactg gcatctccta cgataacttc agataggcct
    50101 agacgtttgg caatttcaaa ggcattactt cggccaggaa caccctgcat aaagcgatag
    50161 gtcgggcgaa gagttgcagt atcaaactcc atactggcat tttgcacaaa ggctgtctca
    50221 ataccgtagg ccttgagttc tggatagtgg gtcgtcgcca tggtcttgat ttgacgcagg
    50281 cgaaggtcct ccagaatagc catggcaagg gctgctccct cttggggatc agtaccagcc
    50341 cccaactcat ccaaaagtaa gagtgaatgt tggttgacct tgccaagaat atccacgata
    50401 ttggtcatat ggctagagaa ggtagacaag ctctgctcaa tagactgctc atctccaata
    50461 tcagcaaaga tttcttcaaa aataccaaca cgacttccct tgtctgctaa aatcggcaat
    50521 cctgactggg ccatgacctg tgtcaagccc agagttttga gcatgatggt cttcccacct
    50581 gtattgggac ctgtaatgac aatagctgtt aaatcttgac caaaatggac atcatttgcg
    50641 acggcatttt tgaccaaagg atggcagaca tggagcagtt gaatctcttg attttctgac
    50701 agctgaggca cgactgcttg tctttcttgg ataaatcgaa ccttggcacg aatcaagtcc
    50761 agatgaccga taatccaagc atcattagca atctcagccg catgagggcg gacacgctca
    50821 gaaatttctt ggagaatgcg aagcatttca tagcgctcat ctgctcgcag actagcaatt
    50881 tcttcgctca gtttgactac ctcacgtggt tcgatataga cggtgtttcc actagcagaa
    50941 atatcatgaa cgacacctgc aatcttattg cggtaggtgt ttttgactgg taaaacctga
    51001 cggccatttc tgctagcaac aattccttcc gtcaacatct gcgctttttg cttgagcaag
    51061 tcttgtaaaa catcgcgtac ctgactctcg ctatcatgta tttttcgacg gattcgcgcc
    51121 aattcttcac tggcaaaatt ttcaatgaaa cccgcatcat taaaggcctg aagatttcct
    51181 tgtaattgcg gaaaatcatg taatttctca aaccaaaggg ctaattcttc caagctgaca
    51241 ttttccagat tggcgtaaaa actttgaagt tctcggctgg caagaagcac gcgtttcaag
    51301 agtaggaact cctcgatatt gagatccgct cccatctcca acctcttgca gactcctgca
    51361 atttccttag ttgagagaat agtaaaatgc ggttgctcga cgaaaagagc ctgcatttct
    51421 ttcatctcag caaaagcctg tttgatttta tctgctttgg cagtcggagc cagctgtctc
    51481 aattgctcca agccctgctc ggtcaacaaa tgaggctcaa acaaggcctt gaccttatcg
    51541 aactctaatg tttctaatat tttcttattc atctttattt ccttgtcttt gaatgtctag
    51601 gtgtggtagc tttttacatt ctgatagatt cgagtcaaat ctgtaaacaa agggctagga
    51661 aaactcctgc cctttttatc cgattaaatt tgtcacccag atttgtttga gccaactggt
    51721 tgttatcggt atgctctgga tgatgtgttt tgcgacgata ctcttttcaa gaggattttg
    51781 tataactgcc atggggatgg tcgccaagat tgtcaaggcc atttgcaaga caaataaggt
    51841 caccaacatg gacaagatac ctgctgaaac ttggaacaac ttaccaccca gttttttgct
    51901 aggaatcaag tgtaagagaa gaccaagcaa acgaccaatg ctatagacaa tcccaaatac
    51961 aagcaagtag ccgatacctg cataaaagac cttatccagc tgaaagagtt gatccgatgg
    52021 gaaaaagaaa gtcccctgac cttcctgcga atttgcataa gggagcaata aatggaattg
    52081 ctctccaagc cccttataaa actggccagc cataaaagcc gatgccatgg ctgaaatcag
    52141 gtaataaacc tgtaagagca ggcctctccg atagccgata taaaatcccc aaaccaagac
    52201 caatagaaga aggagtgaaa tcataaggaa tcctcaatct tgctctgttc ttgcttacaa
    52261 gtcacaagct tgtgacggag ttcttctagc tcttgctcct tatcgtcaaa ttcaatctca
    52321 cggctgagct gagttgataa acagttgact gccaacaaaa gagcgattgt ttcatcatct
    52381 gcgctaggca tttgttcttt aattgcttgg tatttttctg tcgcaacctt agcgatttcc
    52441 tccataaaaa ggttatcatg ttcgcttgtc aaggttaacg attttttccc gaatgtaaat
    52501 ttgaatcgat ttagatttgc cataaaattc acctcacgat attataccaa aattcgctaa
    52561 ttttgtcagt ttttacaaat tttactgctt ttgtggtaca atagaaacta tggcaagtat
    52621 cacactcaca ccaagcgaaa aggatattca ggcttttctt gaacactatc aaactagtct
    52681 ggctcctagc aagaatccct atatccgcta ctttttgaaa ctacctcaag caacggtttc
    52741 tatctatact tctggaaaaa tcttgcttca gggtgaaggg gctgaaaaat acgccagttt
    52801 ctttggctat caagctgtag agcaaaccag cggacaaaat cttcctttaa ttgggacaga
    52861 tgaggtggga aatggttcct actttggtgg gcttgcagtc gtggctgcct ttgtcacacc
    52921 tgaccagcac gactttttac gaaaactcgg tgtgggagat tctaagactc tgaccgacca
    52981 aaagattcgt cagattgctc ctattctcaa ggaaaaaatc cagcaccagg cactccttct
    53041 ctcacccagc aagtacaacg aggtcatcgg agaccgctac aatgctgttt cggttaaggt
    53101 tgccctccat aatcaggcta tctatctcct ccttcaaaaa ggtgttcagc ctgagaaaat
    53161 tgtgattgat gcctttacca gtgctaaaaa ttatgacaag tacttggcac aagaggccaa
    53221 tcgtttcagc aatcctatca gcttagaaga aaaggctgag ggcaaatact tgtctgtcgc
    53281 agtttcttct gtcattgcgc gtgatctctt tctggaaaat cttgaaaatc tgggacgaga
    53341 actgggttat cagcttccaa gtggagctgg aacggcttct gacaaggtgg ctagccagat
    53401 tttgcaagcc tatggtatgc agggactcaa cttctgcgcc aaattgcact ttaaaaacac
    53461 tgaaaaagcg aaaaaacgct tagaaaggta agttatgaat tcatttaaaa atttcttaaa
    53521 agagtgggga ctgttcctcc taattctgtc attactagct ttaagtcgta tctttttttg
    53581 gagcaatgtt cgcgtagaag gacattccat ggatccgacc ctagcggatg gtgaaatcct
    53641 ctttgttgtt aagcacctcc ctattgaccg ttttgatatc gtggtggccc atgaggaaga
    53701 tggcaataag gacatcgtca agcgcgtgat tggaatgcct ggcgacacca ttcgttacga
    53761 aaatgataaa ctctacatca atgacaaaga aacggacgag ccttatctag cagactatat
    53821 caaacgattc aaggatgaca aactccaaag cacttactca ggcaagggct ttgaaggaaa
    53881 taaaggaact ttctttagaa gtatcgctca aaaagcccaa gccttcacag ttgatgtcaa
    53941 ctacaacacc aactttagct ttactgttcc agaaggagaa taccttctcc tcggagatga
    54001 ccgcttggtt tcgagcgaca gccgccacgt aggtaccttc aaagcaaaag atatcacagg
    54061 ggaagctaaa ttccgcttct ggccaatcac ccgtatcgga acattttaag aaacctaaga
    54121 ggccgagaat caccaatctc agcctcttct tctatcgtga gaaaatgatt ggtaactatc
    54181 taaacttacc agaacagaaa cacctcaact ctcacctatt catgcaaagg aattttatgg
    54241 aagtttattt ttcaggaacc attgaacgga ttatttttga aaatcccagc aatttttatc
    54301 gcatcctcct cctagaaatc gacgatacgg acgcagagga ttttgatgat tttgaaatca
    54361 ttgtcacagg aaccatggct gatgtaattg agggcgaaga ctatactttt tgggggcaaa
    54421 ttgtccagca ctccaagtat ggagaacaac tgcaaatcag tcgttatgat cgcgcaaaac
    54481 caactagtaa gggcttggtc aagtactttt caagtagcca tttcaagggg attggtctca
    54541 agacagctca gaaaatcgtg gatacctatg gcgaaaatac cattgacgaa attttgcaac
    54601 acccagaaaa gttagaaggc atcgcaggac tctctgccaa aaatcgcgag gctttcgtct
    54661 ccactctccg tctcaactac ggaacggaga tggttttgtc caaactagcc aactacggca
    54721 ttcccaacaa actagccttt cagattcaag acttttacaa ggaagaaacc cttgatgtgg
    54781 ttgaaaatta tccctaccag ctggttgagg atatcaaggg tttgggcttt acaattgctg
    54841 accaactagc tgaggaacta ggcatcgaaa gtcaggctcc tgaacgcttc cgcgccggtc
    54901 tagttcacag tctttttcag gcctgtatgg aaacagggga cacctatgtt gaagcacggg
    54961 atttgctgga acaaaccctt actctccttg agtcttcccg tcccgtagaa ctagacccca
    55021 gccaagttgc ccaagagctc tcctacctga tcgaagaaga caaggttcag cagattgata
    55081 ccaagatctt tgacaacagc ctctttttcg ctgaggaagg tatccgcagt cacttgattc
    55141 gtatccttga aaaaggaaaa cagaagagcc aagatttaga aactattcaa aaacatatca
    55201 ctactgtcga gcaagaactg gggattgagt atgataacat tcaaaaacaa gctatttgtg
    55261 acgctatcca gaacaaggtc tttatcctga caggtgggcc tggtactggt aagacaactg
    55321 ttatcaatgg aatcatcgct gtttatgccc ttttagaagg acttgacttc aggaagaaaa
    55381 gcaatctgcc cattcttctt gctgctccaa ctggtcgagc tgctcgtcgc atgaatgaat
    55441 tgacaggttt gcctagcgcg accatacacc gccacttggg aatgacaggt gacgatgata
    55501 ccagtcatct ggaagattac ctagatgctg actttatcat cgtggatgaa ttctctatgg
    55561 tggatacttg gctggccaat caactcttct ccaatatctc ttctaacagt aagatcctca
    55621 tcgtaggtga cagtgatcag ctaccttctg tcagtcctgg acaggttcta gcggatctgc
    55681 ttcatattcc tttgattcct cagactcgct tggaaaaaat ttatcgacaa agcgaagaat
    55741 caaccatcgt caccctagct agtcagattc gacagggcat cttgccagtt gatttcaccc
    55801 aaaaaaaagc tgaccgttcc tactttgaaa ttactagtgg ccatattcct gccaccattg
    55861 aaaaaatctt aggtgccgcc ctcagaaatg gtattcctgc ccgtgatatc caagttctgg
    55921 ctcctatgta ccgagggacg gcagggattg atgctatcaa tcagctcatg caagacctgc
    55981 tcaatccccc acaaaaagat caactcagtt ttgaagctcc tcagtgccac tatcgtaagg
    56041 gagacaaggt cattcatttg gttaacgatg ctgaaatcaa tgtctttaat ggggatttag
    56101 gagctatcac agacctgatt cctggtaaat acaccgaatc gaaacaagac gagattgtca
    56161 ttgattttga tggtaatgaa gtctcttacc cccgtaacga atggtacaag attcgcttgg
    56221 cctatgccat gagtattcat aagtctcagg gaagtgagtt cccagttgtc atcctaccga
    56281 ttactagtgc tagccgacgt atgctggagc gaaatctcat ctacacagcc attacacgtg
    56341 ccaaaagcaa actcatctta ctaggcgaat tacaagcctt cgactatgct acccaacaca
    56401 tcggaactgc ccgaaaaacc tatctgattg aacgcttcag tgatctactg gagaatgttg
    56461 aagaaaagca acaagctgtc tctgaaacag tcacatcaag tgcctctgaa caatcctaca
    56521 tcctaaccga agaaaactgg gacagcattc cagccatgat tgggattaca gacacagacc
    56581 tcaaagagat ttttggaaaa tagtgcatca gaaaaaccac caattcaggt gggttttctg
    56641 tttattaaga ttattttact gttgctgtgc ttgtaatcaa ttcaacagct tttttgatag
    56701 tgatatcatg tttcaacatg tcagctgaaa gcaagttttg aacttgtgca acttccatgt
    56761 tgtagtctgc tgccaattgc tcaacttctt tttggatttc ttcttctgaa gcatcaaatc
    56821 cttcagcttt ggcaactgct tcgataacaa ggttagtctt agtacgtgac tcagcttctg
    56881 cttggtattg gttgtgaagg tcttcttgag tagttccagt aatttggaag tacatgtcag
    56941 ggttgatccc ttgacgttgc aagttcccaa ggaattcatt tactgaacgg tgaacttctt
    57001 cgtggatcat ttcttctgga agttctacga tttcagcatt ttctacagct gtatcaattg
    57061 ctgcaccttc aactgcatct ttgtaagctt cttctttagc agcagccaat tctttgcggt
    57121 atttttcttt caagtcagca agtgtttcaa cttcttcatc aatgtctttt gcaagttcat
    57181 cgtcaagagc tggaacttct ttagctttta cttcgtggat agttgtcacg aatttagctt
    57241 ctttacctgc aaggtcttct gcttggtagt cttctgggaa tgttacgata acatcaacgg
    57301 tttcgccagc tgagtgacct accaattggt cttcgaaacc agggatgaat tgacctgaac
    57361 caagtccaag tgagaagttt tcaccttttc caccgtcaaa ttcaacaccg tcgatagaac
    57421 caacgaagtc gatcacaaca gtgtcgccgt cttcagcagc agcttccttg ataaccaatt
    57481 cagccaggtt gttgcgttcg cgttcgatac gctcttcgac atcagcgtca gttacttctt
    57541 tttctacatc aactgatact tcaaggtttt tgtagtcacc caattttact tcaggttttg
    57601 taacaacttc agcagcgata acccagtctt gacctttttc cattgaagtt acgtcaattt
    57661 ttggttgggc aaccacttca agaccagctt cttttacagc tgcttcataa gcgtttggca
    57721 aaagagcgtt cataacgtct tggtaaagtg attcttcacc aaatttttta tcgaagatag
    57781 gacgtggaag gtgaccttta cggaaacctg gaacattaag agatttcttc actgacttga
    57841 agacacggtc caattctggt ttgatttggt cttgagagat agtgaaagtc aagacaccac
    57901 ggtttgtttc tttgttttca aatgatacag acattctgtc atttctcctt aaaatttttt
    57961 aaatacagtc tattataaca ttttctataa ataaaatcta gactaatgca gacaattata
    58021 gacaacaagc ctttttccta act
//
LOCUS       DAVLVZ010000004        52281 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000004 DAVLVZ010000000
VERSION     DAVLVZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 52281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.004"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            2..74
                     /locus_tag="VYN77_000198"
     tRNA            2..74
                     /locus_tag="VYN77_000198"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            99..171
                     /locus_tag="VYN77_000199"
     tRNA            99..171
                     /locus_tag="VYN77_000199"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Lys,seq:ttt)
     gene            177..258
                     /locus_tag="VYN77_000200"
     tRNA            177..258
                     /locus_tag="VYN77_000200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Leu,seq:tag)
     gene            277..349
                     /locus_tag="VYN77_000201"
     tRNA            277..349
                     /locus_tag="VYN77_000201"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Thr,seq:tgt)
     gene            372..443
                     /locus_tag="VYN77_000202"
     tRNA            372..443
                     /locus_tag="VYN77_000202"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Gly,seq:gcc)
     gene            451..536
                     /locus_tag="VYN77_000203"
     tRNA            451..536
                     /locus_tag="VYN77_000203"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:taa)
     gene            546..619
                     /locus_tag="VYN77_000204"
     tRNA            546..619
                     /locus_tag="VYN77_000204"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Arg,seq:acg)
     gene            635..708
                     /locus_tag="VYN77_000205"
     tRNA            635..708
                     /locus_tag="VYN77_000205"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:669..671,aa:Pro,seq:tgg)
     gene            714..787
                     /locus_tag="VYN77_000206"
     tRNA            714..787
                     /locus_tag="VYN77_000206"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Met,seq:cat)
     gene            801..874
                     /locus_tag="VYN77_000207"
     tRNA            801..874
                     /locus_tag="VYN77_000207"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Ile,seq:cat)
     gene            885..974
                     /locus_tag="VYN77_000208"
     tRNA            885..974
                     /locus_tag="VYN77_000208"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Ser,seq:tga)
     gene            985..1058
                     /locus_tag="VYN77_000209"
     tRNA            985..1058
                     /locus_tag="VYN77_000209"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1019..1021,aa:Met,seq:cat)
     gene            1062..1134
                     /locus_tag="VYN77_000210"
     tRNA            1062..1134
                     /locus_tag="VYN77_000210"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1095..1097,aa:Phe,seq:gaa)
     gene            1154..1224
                     /locus_tag="VYN77_000211"
     tRNA            1154..1224
                     /locus_tag="VYN77_000211"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Gly,seq:tcc)
     gene            1260..1333
                     /locus_tag="VYN77_000212"
     tRNA            1260..1333
                     /locus_tag="VYN77_000212"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1294..1296,aa:Ile,seq:gat)
     gene            1340..1427
                     /locus_tag="VYN77_000213"
     tRNA            1340..1427
                     /locus_tag="VYN77_000213"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1374..1376,aa:Ser,seq:gct)
     gene            complement(1561..1998)
                     /locus_tag="VYN77_000214"
     CDS             complement(1561..1998)
                     /locus_tag="VYN77_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1557739.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKKEPEKFFELIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEA
                     TNNLIKLIKCNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(2205..3140)
                     /locus_tag="VYN77_000215"
     CDS             complement(2205..3140)
                     /locus_tag="VYN77_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1557740.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKA
                     KAASLFKATAPITRNELIIEIWRAFFETPSEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(3137..3997)
                     /locus_tag="VYN77_000216"
     CDS             complement(3137..3997)
                     /locus_tag="VYN77_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1557741.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVNLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            4105..6267
                     /locus_tag="VYN77_000217"
     CDS             4105..6267
                     /locus_tag="VYN77_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1557742.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            6359..7618
                     /locus_tag="VYN77_000218"
     CDS             6359..7618
                     /locus_tag="VYN77_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1557743.1"
                     /translation="MEWYKKIGLLVTTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            7632..8498
                     /locus_tag="VYN77_000219"
     CDS             7632..8498
                     /locus_tag="VYN77_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1557744.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAAFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            8512..9345
                     /locus_tag="VYN77_000220"
     CDS             8512..9345
                     /locus_tag="VYN77_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1557745.1"
                     /translation="MKQDERKALIGKYILLVLGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     K"
     gene            9346..9426
                     /locus_tag="VYN77_000221"
                     /pseudo
     CDS             9346..9426
                     /locus_tag="VYN77_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            9703..11145
                     /gene="gtfA"
                     /locus_tag="VYN77_000222"
     CDS             9703..11145
                     /gene="gtfA"
                     /locus_tag="VYN77_000222"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1557746.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            11283..12476
                     /locus_tag="VYN77_000223"
     CDS             11283..12476
                     /locus_tag="VYN77_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1557747.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            13163..15142
                     /locus_tag="VYN77_000224"
     CDS             13163..15142
                     /locus_tag="VYN77_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1557748.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            15209..16705
                     /locus_tag="VYN77_000225"
     CDS             15209..16705
                     /locus_tag="VYN77_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1557749.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            16705..17631
                     /locus_tag="VYN77_000226"
     CDS             16705..17631
                     /locus_tag="VYN77_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1557750.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            17640..18707
                     /locus_tag="VYN77_000227"
     CDS             17640..18707
                     /locus_tag="VYN77_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1557751.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRSQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            18718..19644
                     /locus_tag="VYN77_000228"
     CDS             18718..19644
                     /locus_tag="VYN77_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1557752.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(19719..21053)
                     /locus_tag="VYN77_000229"
                     /pseudo
     CDS             complement(19719..21053)
                     /locus_tag="VYN77_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(21208..21918)
                     /gene="treR"
                     /locus_tag="VYN77_000230"
     CDS             complement(21208..21918)
                     /gene="treR"
                     /locus_tag="VYN77_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1557753.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKEPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            22103..24070
                     /gene="treP"
                     /locus_tag="VYN77_000231"
     CDS             22103..24070
                     /gene="treP"
                     /locus_tag="VYN77_000231"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1557754.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKIEGDTNLQAEFVAQEEAEFVSHEPVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            24248..25873
                     /gene="treC"
                     /locus_tag="VYN77_000232"
     CDS             24248..25873
                     /gene="treC"
                     /locus_tag="VYN77_000232"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1557755.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEIVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            26129..26377
                     /locus_tag="VYN77_000233"
     CDS             26129..26377
                     /locus_tag="VYN77_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1557756.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26584..27378
                     /gene="racE"
                     /locus_tag="VYN77_000234"
     CDS             26584..27378
                     /gene="racE"
                     /locus_tag="VYN77_000234"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1557757.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            27375..28346
                     /locus_tag="VYN77_000235"
     CDS             27375..28346
                     /locus_tag="VYN77_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1557758.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            28322..28843
                     /locus_tag="VYN77_000236"
     CDS             28322..28843
                     /locus_tag="VYN77_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1557759.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28840..29301
                     /locus_tag="VYN77_000237"
     CDS             28840..29301
                     /locus_tag="VYN77_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1557760.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            29292..30032
                     /gene="xerD"
                     /locus_tag="VYN77_000238"
     CDS             29292..30032
                     /gene="xerD"
                     /locus_tag="VYN77_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1557761.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            30032..30760
                     /locus_tag="VYN77_000239"
     CDS             30032..30760
                     /locus_tag="VYN77_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1557762.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30779..31348
                     /locus_tag="VYN77_000240"
     CDS             30779..31348
                     /locus_tag="VYN77_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1557763.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            31335..32057
                     /locus_tag="VYN77_000241"
     CDS             31335..32057
                     /locus_tag="VYN77_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1557764.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            32057..32299
                     /gene="yidD"
                     /locus_tag="VYN77_000242"
     CDS             32057..32299
                     /gene="yidD"
                     /locus_tag="VYN77_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1557765.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(32524..33489)
                     /locus_tag="VYN77_000243"
     CDS             complement(32524..33489)
                     /locus_tag="VYN77_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1557766.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(33585..34337)
                     /locus_tag="VYN77_000244"
     CDS             complement(33585..34337)
                     /locus_tag="VYN77_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1557767.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(34334..35290)
                     /locus_tag="VYN77_000245"
     CDS             complement(34334..35290)
                     /locus_tag="VYN77_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1557768.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(35280..36239)
                     /locus_tag="VYN77_000246"
     CDS             complement(35280..36239)
                     /locus_tag="VYN77_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1557769.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(36662..37363)
                     /gene="galE"
                     /locus_tag="VYN77_000247"
     CDS             complement(36662..37363)
                     /gene="galE"
                     /locus_tag="VYN77_000247"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1557770.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVG
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            37764..38828
                     /gene="pepA"
                     /locus_tag="VYN77_000248"
     CDS             37764..38828
                     /gene="pepA"
                     /locus_tag="VYN77_000248"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1557771.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            38850..39146
                     /locus_tag="VYN77_000249"
     CDS             38850..39146
                     /locus_tag="VYN77_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1557772.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            39828..40271
                     /locus_tag="VYN77_000250"
     CDS             39828..40271
                     /locus_tag="VYN77_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1557773.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            40275..41039
                     /locus_tag="VYN77_000251"
     CDS             40275..41039
                     /locus_tag="VYN77_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1557774.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            41054..41782
                     /locus_tag="VYN77_000252"
     CDS             41054..41782
                     /locus_tag="VYN77_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1557775.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            41775..43295
                     /locus_tag="VYN77_000253"
     CDS             41775..43295
                     /locus_tag="VYN77_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1557776.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            43640..44446
                     /locus_tag="VYN77_000254"
     CDS             43640..44446
                     /locus_tag="VYN77_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1557777.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLPN"
     gene            complement(44612..45148)
                     /locus_tag="VYN77_000255"
     CDS             complement(44612..45148)
                     /locus_tag="VYN77_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1557778.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            45281..46171
                     /locus_tag="VYN77_000256"
     CDS             45281..46171
                     /locus_tag="VYN77_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1557779.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALSRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            46415..46768
                     /locus_tag="VYN77_000257"
     CDS             46415..46768
                     /locus_tag="VYN77_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1557780.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            46769..47806
                     /locus_tag="VYN77_000258"
     CDS             46769..47806
                     /locus_tag="VYN77_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1557781.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(47852..48859)
                     /gene="galR"
                     /locus_tag="VYN77_000259"
     CDS             complement(47852..48859)
                     /gene="galR"
                     /locus_tag="VYN77_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1557782.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            49023..50201
                     /locus_tag="VYN77_000260"
     CDS             49023..50201
                     /locus_tag="VYN77_000260"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1557783.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGFVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAFDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEVFKEAVGKHYEEVVGYAPSFYIAEVAGGTHVLD
                     "
     gene            50220..51701
                     /locus_tag="VYN77_000261"
     CDS             50220..51701
                     /locus_tag="VYN77_000261"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1557784.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWETYVHYPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            51771..52175
                     /locus_tag="VYN77_000262"
     CDS             51771..52175
                     /locus_tag="VYN77_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1557785.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
BASE COUNT        14949 a         9687 c        11881 g        15764 t
ORIGIN      
        1 aggtcccgta gtgtagcggt tatcacgtcg ccctgtcacg gcgaagatcg cgggttcgat
       61 tcccgtcggg accgtttaag gtaacgcaag ttattttaga ctcgttagct cagttggtag
      121 agcaattgac ttttaatcaa tgggtcactg gttcgagccc agtacgggtc atatatgcgg
      181 gtttggcgga attggcagac gcaccagatt taggatctgg cgcttaacgg cgtgggggtt
      241 caagtccctt aacccgcatt aagatataac aaatgagccg gcttagctca gttggtagag
      301 catctgattt gtaatcagag ggtcgcgtgt tcaagtcatg tagccggcat ttttagatag
      361 aagaaaacag tgcgaacgta gttcagtggt agaacaccac cttgccaagg tgggggtcgc
      421 gggttcgaat cccgtcgttc gcttagagag gccggggtgg cggaactggc agacgcacag
      481 gacttaaaat cctgcgattg gtaacgatcg taccggttcg attccggtcc tcggcataat
      541 atggagcacc cttagctcaa ctggatagag tacctgacta cgaatcaggc ggttagaggt
      601 tcgactcctc tagggtgcat ttttctattt aactcgggaa gtagctcagc ttggtagagt
      661 acttggtttg ggaccaaggt gtcgcaggtt cgaatcctgt cttcccgatt catggcggtg
      721 tagctcagct ggctagagcg tccggttcat acccgggagg tcgggggttc gatccccttc
      781 gccgctataa tgatcttgtc ggacctttag ctcagctggt tagagctctc ggctcataac
      841 cgagtggtcg taggttcaag tcctacaagg tccatttgaa tattggagga ttacccaagt
      901 ccggctgaag ggaacggtct tgaaaaccgt caggcgtgta aaagcgtgcg tgggttcgaa
      961 tcccacatcc tccttttata ttaacgcggg atggagcagc tcggtagctc gtcgggctca
     1021 taacccgaag gtcgtaggtt caaatcctgc tcccgcaata aggctcggta gctcagttgg
     1081 tagagcaatg gattgaagct ccatgtgtcg gcggttcgat tccgtctcgc gccattttat
     1141 tgatagtatt tatgcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
     1201 cgagttcgat tctcgtcacc cgctttgaac tttgttcaaa ttaccaagtt ttttaacttg
     1261 ggcgcgtagc tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt
     1321 ccactcgtgc ccattaattg gagaattact caagaggctg aagaggacgg tttgctaaat
     1381 cgttaggtcg ggtaactggc gcaagggttc gaatccctta ttctccgttt taatgagagt
     1441 ttgtaactct cttttttatt tttagagaca ataaagcttc agatccgtaa aacttaattg
     1501 aattactaat ctgaagttat ttttatggct ctttgtcaac tgtagtgggt tgaagaaaag
     1561 ctaagctcga gaaaggacaa atttcgtcct ttcttttttg atgttcaaag cgataaaaat
     1621 ccgtttttta aagttttcaa agtttcgaaa accaaaggca ttgcacttga taagtttgat
     1681 gagattattg gtcgcttcca gtttggcgtt agaatagtgt agttgaaggg cgttgacaat
     1741 cttttcttta tctttgagga aggttttaaa gacagtctga aaaataggat gaacctgctt
     1801 aagattgtcc tcgataagtt cgaaaaattt ctctggttcc tttttcttca ttagacttcc
     1861 acaatcaggg caagatggag cctcataatc cagcttagcg ataatttctt tgtgggtatc
     1921 catattgatg atatctagaa tcttgatgtt tgggtcttta atatcgagca gttttgtgat
     1981 aaaatgtaat tgttccatat gaatctttct aatgatggtt ttgtcgcttt tcattatagg
     2041 tcatatggga ctttttttct acaacaaaat aggctccata atatctataa gggatttacc
     2101 cactacaaat attatagagc caataaaact tcagatccgt aaaacttaat tgaattacta
     2161 atctgaagtt atttttatat cgtttatatt ataattgtgt tattttactt ccaactattg
     2221 agagaaattt cgccactatt tagccagatt tcttttccgt tatcacattg aactaaaagt
     2281 tttccattct ctgagatgtc tttagcaagt cccttgtagt ctttttgctc tagtgtgaaa
     2341 gtgacttctt ttcctagaat gaatgactgt tttttgtata ggtataatag ctcttctgat
     2401 ggtgtttcga agaaagcacg ccagatttct atgatcaatt cattccttgt tataggagct
     2461 gtagctttaa ataagctggc agcttttgct tttaattcct gagggaagtc tttaatagtg
     2521 aaattgatac ctactccaat aatgatatct gtgactaagc cagtttctac agaggtcatt
     2581 gcttcagtaa ggattcctcc aattttatga ttgtttagat agatatcatt gacccatttt
     2641 atgtcgacat ctattaaagt taggttctta atggctttgt agacagctcc agctacaagt
     2701 agtgtgtagg atggtaattt gtcatagggg agatttggtt taagatggag tgtcatataa
     2761 ataccacctt gtggtgagta gaaggaacgt tgaaaacggc ctcggcctgc tgtttgatag
     2821 gaagctagat agagggtatt tgcttcatgg cctaaatcaa ttgcttcttt tgcatctagt
     2881 tgtgttgatt ttgtttcggg tttaaagctg actttaattg gaagattttc ttctagaatc
     2941 tctggaagaa taaggtcacc attcattagt ttatatcctc tatttttgat actatcaatt
     3001 tcaatgcctt cttgttctag tcgcttgata gctttccaaa ttgatgttcg gcttagggat
     3061 agtttttctg cgattttttc tccgctgata tagtcggttt ctttagatag gatttggtag
     3121 acagcttggt aggatttcat aatgttgcct ttctcactag ttggtattga gagtattctt
     3181 ttcttgtatg acttggagtc tgattaaagt attgtttata agctttcgaa aaatggagtg
     3241 gatctgaaaa acctaccgag tatgcaatta ccttgatgga ctcttgggta ttttcgagaa
     3301 gttgtctagc tcggtgcatt cgaacgtaga gtaggtattc tttgggtgat aaggtattaa
     3361 attctttgaa tacgcttgat aagtagcttc tgtgaacgga tagttctttt gctaaatctt
     3421 gaattgtaag tgattgagga tagtggctat caattaatcg tttgcattca agatagagtt
     3481 ggtgggttga tgaaatattc ttttttttct gattgggagc aatagttccc agatgaaaca
     3541 tcagttcatg aagttgtccc atgatatgga gttgagctaa ttcacttgat tttgtaatct
     3601 gagcgaagcg gacaatgtct gagatgagtt ttgcagtagt ctgggtatga caagtttcag
     3661 attggatgag ataggattga tcagaaattt gagaaagagc aaaataatca ggggatttcc
     3721 ctccagtgat tcctaaccag tagtaggccc aaggttcttt actatctgct tgataaaagg
     3781 ttagttcctc tggttttaat agaaagaaat ctccttcttt taaattaaca attttaccct
     3841 tgtaatgaaa ttttccttgt cctttagtaa tgtaatgtag gacgtatgta tcacgaatgg
     3901 ctggaccaaa agagtaatta ggtgtgcatt cctcatatcc ataaaagctt agggcaaggt
     3961 cgattgttcc agtctggtat tctgaaaaaa ctaacatgtg ctacctccta cctaacattt
     4021 taccatattc ttgagacatt tttctatttt agaaagcgat ttcaggtgat aaaatataat
     4081 caataaagtg atgaggtgaa gtaaatggga gttaggatag agaataatct attttatgtt
     4141 gagagtaaaa atctaagttt gattattgaa aatcgaaatg gctacttact tttgaaacat
     4201 ttaggaaaga ctattaagaa ctataaaggt tccaatagtg tttatgaacg agaccatgcc
     4261 ttttcaggaa atccaacggc tactaatcga acctttagtt tagatactca gcgacagatt
     4321 tttggacaac atggcttagg agattttagg aaaccaacca tacaggttca gcatagtgta
     4381 actgaagtaa cagactttcg atttgtagaa gcaaagattt taaaaggtca gaatggtcca
     4441 cagggcttac cttctccaca tagcatggac gatacagaga ctcttgtctt aatgttagaa
     4501 gattctaagg ctcaacttag tctgactttg tattatacta cttttaataa tgatgcgact
     4561 attgctagct acagtaaatt agataataat agtaatcagg aagttgtcat ccataaagat
     4621 ttttctttta tggctgattt tccagctgca gattacgaaa tagtaactct gcagggtgct
     4681 tatgctcgtg aaaagactgt tagacgtcaa caggtagaac aaggaatctt ttcgattagc
     4741 tcaaaccgag gtgcttctgg tcatgctcaa acaccagctc ttctactatg cgaacaagga
     4801 gtcacagagg atgctgggaa tgtgtttgct attcaactaa tgtatagtgg caactttgaa
     4861 gcttttgttc aaaaaaatca attgaatgaa gttcgggtgg ctattggcat taatccagaa
     4921 aacttttctt ggaagttagc ccctgaggaa tactttgaaa caccggtagc tttagtgact
     4981 cattcagatc agggattaac tggtattagt catgaaagtc agaattttgt actgaagcac
     5041 attattctaa gtgaattttc taaaaaagaa cgtccaattc taatcaataa ctgggaagct
     5101 acttactttg actttcagag agaaaaactg ttagagttag cagatgaagc taagaaagtt
     5161 ggcattgaac tttttgtatt agatgatggt tggtttggca atcgttttga tgataatcgt
     5221 gctttaggtg attgggttgt taatgaggaa aaactgggtg gaagtctaga aagtctgatt
     5281 tcagctatcc atgaaagagg tttgcagttt ggactttggt tagaacccga aatgatttct
     5341 gtagatagtg atttgtatcg tcaacatcct gactgggcta ttcaggttcc tgattatgag
     5401 catacttatt ctcggaatca attagtactt aatcttgcca atcctcaggt agtagaatac
     5461 ttgaaaagtg tcttagatca actcctatct tatcatgaga ttgattacat taaatgggat
     5521 atgaaccgca atatcactaa gctagggaat ggattaactt atctagagac acagatgcaa
     5581 tctcatcagt acatgctggg gctttacgaa ctcgtttctt atctgacaga gaagcacagc
     5641 catattctct ttgagtcctg ctctggtggt ggtggacgaa atgatcttgg tatgatacgc
     5701 tatttcccac aagtctgggc tagtgataat actgatgcca ttgcacgttt accaattcaa
     5761 tacggttcat cctatctcta tccaaccatt tctatggggg ctcatgtgtc agcagtaccg
     5821 aatcatcaga tgggacgaat gacaccatta gaaacacgtg gccatgtagc aatgatggga
     5881 aatttgggct atgagcttga tttgacaaat ttatcagatg aagagaaagc tacgattgct
     5941 aatcaggtga acttgtataa agaattacga ccagtagttc agttaggaca gcagtataga
     6001 ctaattaatc ctgatactgc atccaatgaa gcagctgtac aatttaatta cggaaatcaa
     6061 acgattgtaa cctacgttcg cgttttatct gttgtagaga ccatggaaac aactttaaaa
     6121 ttaaaagatt tggatgaaga gggactatat aaattacagg aaaatggcga agtttactca
     6181 ggtgcagaac taatgtatgc gggcttaact gttatcttat cccaaggaga ttttttgagt
     6241 agacagtata tttttagaaa actatgatat gtactgaccc caaaagttag atttattctg
     6301 tctaactttt ggggtgcagt tcaataaggg tgtataaatt tataaaggag gctttctaat
     6361 ggaatggtat aaaaaaatcg gacttcttgt aactacaggt ttagctttgg ttgggctcgg
     6421 cgcttgctcc aactatggta aatctgcgga tggcacagtg accatcgagt atttcaacca
     6481 gaaaaaagaa atgaccaaaa ccttggaaga aatcactcgt gattttgaga aggaaaaccc
     6541 taagatcaag gtcaaagtcg tcaatgtacc aaatgctggt gaagtattga agacacgcgt
     6601 tctcgcagga gatgtgcctg atgtggtcaa tatttaccca cagtccatcg aactgcaaga
     6661 atgggcaaaa gcaggtgtct ttgaagattt gagcaacaaa gactacctga aacgcgtgaa
     6721 aaatggctac gctgaaaaat atgctgtaaa cgaaaaagtt tacaacgttc cttttacagc
     6781 taatgcttat ggaatttact acaacaaaga taaattcgaa gaactgggct tgaaggttcc
     6841 tgaaacctgg gatgaatttg aacagttagt caaagatatc gttgctaaag gacaaacacc
     6901 atttggaatt gcaggtgcag atgcttggac actcaatggt tacaatcaat tagcctttgc
     6961 gacagcaaca ggtggaggaa aagaagcaaa tcaatacctt cgttattctc aaccaaatgc
     7021 cattaaattg tcggatccga ttatgaaaga tgatatcaag gtcatggaca tccttcgcat
     7081 caatggctct aagcaaaaga actgggaagg tgctggctat accgatgtta tcggagcctt
     7141 cgcacgtggg gatgtcctca tgacaccaaa tgggtcttgg gcgatcacag cgattaatga
     7201 acaaaaaccg aactttaaga ttgggacctt catgattcca ggaaaagaaa aaggacaaag
     7261 cttaaccgtt ggtgcgggag acttggcatg gtctatctca gccactacca aacatccaaa
     7321 agaagccaat gcctttgtgg aatatatgac ccgtccagaa gtcatgcaaa aatactacga
     7381 tgtggacgga tctccaacag cgatcgaagg ggtcaaacaa gcaggagaag attcaccgct
     7441 tgctggtatg accgaatatg cctttacgga tcgtcacttg gtctggttgc aacaatactg
     7501 gaccagtgaa gcagacttcc ataccttgac catgaactat gtcttgaccg gtgataaaca
     7561 aggcatggtc aatgatttga atgccttctt taacccgatg aaagcggatg tggattagga
     7621 gtagagagac tatgaaaaaa gtattacaaa aatattgggc atgggctttt gtggtcatcc
     7681 ccctcttgtt acaagcaatt ttcttctatg tgccgatgtt tcaaggagcc ttttacagtt
     7741 ttaccaactg gacaggattg acttataact acaaatttgt tggcttaaac aactttaagc
     7801 tcctcttcat ggatccaaaa ttcatgaatg cgattggctt taccgcaatc attacgattg
     7861 ccatggtggt tggtgagatt gcactcggga tcttcattgc gcgtgtcttg aattctaaaa
     7921 tcaaaggcca aaccttcttc cgtgcttggt tcttcttccc agctgtttta tctggtttga
     7981 cagtggcttt gatcttcaag caagtcttca actacggtct tccagcgatt gggaatgccc
     8041 ttcatattga atttctccaa accagtcttt tagggactaa gtggggagca atctttgcgg
     8101 ctgcctttgt ccttctttgg caaggggtgg ctatgcccat catcatcttc ctagctggtt
     8161 tgcaatctat tccaactgag attacagagg cagcaaggat tgatggtgcg actagcaagc
     8221 aagttttctg gaacattgaa ttgccttact tgctaccaag tgtctctatg gtctttatcc
     8281 tagccctaaa aggtgggctg actgcctttg accaagtctt tgccatgacc ggtggtggtc
     8341 caaacaatgc cacaacctca cttgggctct tggtttataa ctatgccttt aaaaacaacc
     8401 aattcggtta tgccaatgcc attgccgtaa tcttgttcct cttaattgta gtgatttcga
     8461 tcatccaatt gagagtatct aagaaatttg aaatttaaga ggagaagcat gatgaaacaa
     8521 gatgaaagaa aagccctgat tggcaaatac attctattgg ttctaggatc ggttctgatt
     8581 ttagtgccgc tccttgctac cctctttagt tccttcaaac ccactaagga tattgtagat
     8641 aatttctttg gctttccaac caacttcaca tgggacaact ttagccgtct cttagctgat
     8701 gggattggag gctattattg gaactctgtc gtcatcactg tcttgtcttt acttgcagta
     8761 atgatcttta tccctatggc agcctactcc atcgctcgca atatgagtaa aagaaaagcc
     8821 tttaccatca tgtataccct cttaatcctc ggaatattcg tacctttcca agtcatcatg
     8881 attccgatta cggttatgat gagtaaactc ggtttggcta atacctttgg tttgatcttg
     8941 ctctacttga cctatgcgat tccacagacc ctctttctct atgttggcta tatcaaaatc
     9001 tcgattccag aaagtctgga tgaagcagca gagatcgatg gggctaatca atttacaacc
     9061 tatttccgca tcatcttccc aatgatgaaa ccgatgcatg cgacaaccat gatcatcaat
     9121 gccctttggt tctggaatga cttcatgttg ccactccttg tattgaaccg ggattccaaa
     9181 atgtggactc tgcctttgtt ccaatacaac tacgcaggcc aatatttcaa cgactacgga
     9241 ccaagctttg cctcttacgt ggtcggcatt atcagtatca ccattgtcta tctcttcttc
     9301 caacgccata tcatcgcagg aataagcaac ggggcagtga agtaatactc aatgaaaatc
     9361 aaagagcaaa ctaggaagct agccgcaggt tgctcaaaac actgttttga ggttgtggat
     9421 gaaactgacg aagtcagtaa ccatacctac ggtaaggcga cgctgacgtg gtttgaagag
     9481 attttcgaat agtataagaa gagctcatgg aatctatagt ctttgacaca gaggaagagt
     9541 tcagcttgtt gataattcta tgtagaaaac aagaaagtag tactttgttt atcacagtct
     9601 atattagtac gagtttgatg ataaatagag atttaaaatg aatttgattt acgaagccag
     9661 atcatatgag acttggcttc aattagaaaa aggagagtaa tgatgccaat tcaaaataaa
     9721 accatgttga ttacctattc tgatagcctt ggaaataatc ttaaagactt atatgataat
     9781 ttggaagagc attttggaga tgctattgga ggagttcacc ttttaccatt tttcccatca
     9841 acaggtgatc gtggatttgc gccagttgac tacgacgaag tggactcagc ttttggtgat
     9901 tgggaggatg ttaagcgttt aggtgagaga tattatctta tgtttgattt tatgattaat
     9961 catatttctc gtcaatccaa gtattataag gactatcaag aaaaacatga agccagtgaa
    10021 tttaaagatc tctttttaaa ctgggataag ttttggccag aaaaccgtcc gacacagtct
    10081 gatgtagatt taatttacaa gcgtaaggat cgtgcaccaa agcaagagat tgtgtttgaa
    10141 gatggttcag tggaacattt gtggaatacc tttggtgagg agcagattga tcttgatgtg
    10201 accaaagaag taactatgga atttatccgt aagaccattc agcacttggc aagtaatggg
    10261 tgtgatttga ttcgtctaga tgcctttgct tatgcagtga agaaattgga tactaatgat
    10321 ttctttgtgg aaccagatat ttgggattta ttggacaaag ttcgagatat cgctgccgag
    10381 tatgggacag agcttttacc tgagattcat gaacactatt cgattcagtt taaaatagca
    10441 gaccatgatt actatgttta tgattttgct cttccaatgg tgacacttta tactctttac
    10501 agttccagaa cagagcgttt ggctaagtgg ttaaagatga gcccgatgaa gcaatttacg
    10561 acgctagata cccatgatgg gattggagta gtagatgtca aggatatcct gaccgatgag
    10621 gagattgact atgcttcaaa tgaactctat aaggttggag ccaatgtcaa acgtaagtac
    10681 tctagtgccg agtataacaa cttagatatc taccaaatca attcaaccta ctattcagcg
    10741 cttggagatg atgatgtcaa gtattttctc gctcgtctaa ttcaagcttt tgccccaggt
    10801 attcctcagg tttactatgt gggtctatta gcaggcaaga atgacttgaa attattagaa
    10861 gaaactaaag aaggtcgaaa tattaatcgt cattactata gcaacgagga aatagcaaaa
    10921 gaagtgcaac gacctgttgt gaaggccctt ctcaatctat tttctttccg taaccgttca
    10981 gaagcctttg atctagaagg gactactgag atagagacac caacagctca cagcattgta
    11041 atcaaacgtc aaaataaaga taagtccgta acagcagtag tagaaattga tttgcaaaat
    11101 cagacttatc gggtaattga gaatggagtt gaagtatcat tttgaagcct tgatatggtg
    11161 gataaaatag ggtttttatt ttggaaaacg tttcctttgt tttcaaattg ctaaaaaagt
    11221 ggtacaatat aggatagctt actattatct gaatcagctg atttggagag aaaggattca
    11281 ttttgaaatc aataggcttt attgaaaagc tgaaggggtt gtctagtaaa gagctgattt
    11341 tattgggaat tatcctaagt atctttttac ccttttatct ttttgtagtt gtactctgtt
    11401 tatatattat cagtttgatt tttacaggag acatgaaaag tattcttcag aaaatggggg
    11461 agcatccgat gctgcttctt tttcttagct atagtactgt tatatccatt cttgcacaaa
    11521 attggatggg tcttgtggct tcagtaggaa tgtttctatt tactattttc tttttgcact
    11581 atcagtcgat tttatcccat aaattctttc gattgatttt gcagttcgtc ttgtttggta
    11641 gtgtcttgtc agctgctttt gccagtttag aacatttcca aattgtgaag aaatttaact
    11701 atgcttttct ttcacccaat atgcaggtgt ggcatcagaa ccgggcagaa gtgaccttct
    11761 ttaatcctaa ttattatgga attatttgtt gtttctgtat tatgattgct ttctatctgt
    11821 ttacaacgac caagttgaat tggttgaaag tattctgtgt gattgcaggc tttgttaatc
    11881 tctttggttt gaactttact caaaatcgaa ctgcctttcc tgctattatc gctggagcaa
    11941 ttatctatct ctttacgact attaaaaact ggaaggcctt ttggcttagt attggggtct
    12001 tcgcgattgg tttgagtttc ctcttttcta gtgatttggg agttcgaatg ggtactttag
    12061 actcttctat ggaagaacgc atttctatct gggatgctgg gatggccttg tttaagcaaa
    12121 atcctttttg gggtgaaggg ccattgacct atatgcactc ttatcctcgg atacatgctc
    12181 cttatcatga acatgcccac agtctttata ttgatacgat tctgagttac ggaattgtgg
    12241 gtaccatttt attagttttg tcttctgttg ctcctgttcg cttgatgatg gatatgagtc
    12301 aggagtcggg gaaacgtccg attatcggcc tttatctatc tttccttaca gtggttgctg
    12361 tgcacggaat ttttgacttg gctctcttct ggattcagtc aggctttatt ttcttgctag
    12421 ttatgtgcag tattccattg gagcatcgaa tgttggtatc ggacatgacg gattaagttt
    12481 tataagtttg aaatcttcta ccttgggtag gagatttttt tatacgatgg gcatgtcgtg
    12541 catctgaggt gtaagtcctc tgtagggatc cactaccggt gaatacaata gcgctcccca
    12601 agcctgacta tcgtgaggta gcggattaaa atggtctggg gatagatcgt tttaagtctg
    12661 acgcaggaaa taagaattgt cagagaaagc aatagtgaaa tcgtaggcta tgaatagaaa
    12721 cgtgatagcg aggagcatat agtggataag gtagcgagaa attttaaagt ataaaagata
    12781 gtcttaatct atatatgtaa atcacaaaag taattgaatt cggaataagg ttggtgtgaa
    12841 aagacatagt aaactgaatc ttgaatacta gaatgatact tctacaatat tgttataatt
    12901 taatttgaat tccaaaatca cattgttccg tccgtatttc attttactgt atgtcttcct
    12961 aaactccaaa gtttctgcgt caaatttcct attttcactg tagtctttga cacttcgaac
    13021 gacaatttga atcatatatt caaaaaaaaa tgaaaaattt gtgccaacaa gcctaaatat
    13081 tctgaaaatt tgtttgattt ttaatggata atgtgatata atggttgtca atctataatt
    13141 cacataaaca gaggagtaga aaatgaaaaa aaatcgtgta tttgctacag caggtcttgt
    13201 tttattagca gcaggtgtac ttgcagcatg cagttcttca aaatcatctg attcatcagc
    13261 ccctaaagct tatggctatg tttatacagc agacccagaa accttggact acctgatttc
    13321 aagtaaaaat agtacaacag tagtgacttc aaatgggatt gatggtttat tcactaacga
    13381 taattacggt aatcttgctc ctgcagttgc agaggattgg gaagtctcta aggatggttt
    13441 gacctacact tataagattc gtaaaggggt taaatggttt acctctgatg gagaagaata
    13501 tgcagaggtg acggctaaag attttgtgaa cggtttaaaa cacgcagcag ataaaaaatc
    13561 agaagctatg tatttagctg aaaattcggt taaaggcttg gcagattatc tatcaggaac
    13621 ttcaacagat ttttcaacag ttggtgtcaa ggcggttgat gattatacgt tacaatacac
    13681 tttgaaccag cctgaaccgt tctggaactc taagttgacc tattctattt tctggcctct
    13741 gaatgaagaa ttcgaaacat caaaaggaag cgattttgct aaaccaacag atccgacatc
    13801 cttgctttat aatggtccat tcttgttgaa agggttgact gcaaaatctt ctgtagagtt
    13861 tgtaaaaaat gagcaatatt gggataaaga aaatgtccac ctagatacta tcaatctagc
    13921 ttactatgat ggatcagatc aggagtcgct agagcgtaac ttcactagtg gagcttatag
    13981 ttatgcccgt ctttacccta ccagctccaa ctattctaag gttgcagaag aatacaagga
    14041 caatatctat tacacacaat caggctctgg gattgctggt ctgggtgtga atattgatcg
    14101 ccaaagttac aactatactt ctaaaactac agattcagag aaagtagcta ctaagaaggc
    14161 attgcttaac aaagatttcc gtcaagcctt gaattttgct cttgatcgct cagcttactc
    14221 agctcaaatc aatggtaaag atggagcagc tttagcagtt cgtaatttat ttgtaaaacc
    14281 agactttgtt tcagctggtg agaagacctt tggtgattta gtcgctgctc aacttcctgc
    14341 ttatggtgat gagtggaaag gtgtgaattt agctgatggg caggatggtt tattcaatgc
    14401 tgacaaggcc aaggcagagt ttgcgaaagc taagaaagct ttagaagcag acggcgttca
    14461 gtttcctatt catctggacg ttccagtaga ccaagcatca aaaaactaca tatctcgtat
    14521 tcagtccttt aaacaatctg tagaaacagt tcttggtgtt gaaaatgtcg ttgttgatat
    14581 tcaacaaatg acaagtgatg aattccttaa tattacttac tatgctgcca atgcttcatc
    14641 tgaggattgg gatgtatcgg gaggagtttc atgggggcca gactatcaag acccatctac
    14701 ttacctggat attttaaaaa caactagcag tgaaactaca aaaacatatt taggatttga
    14761 taatccaaat agcccttcag tagttcaagt tggtttgaaa gaatacgata aattagttga
    14821 tgaagctgcc aaagagacaa gcgacttgaa tgtccgttat gaaaaatatg cagcggctca
    14881 agcatggttg acagatagtt cactctttat tcctgctatg gcttcttctg gtgcagcacc
    14941 agtgctttca cgaattgttc catttactgg agcttctgcg caaacaggct ctaaggggtc
    15001 agatgtttac ttcaaatatt tgaaattaca agataaagtg gtgactaagg aagagtatga
    15061 aaaagctcgt gaaaaatggt tgaaagaaaa agctgaatca aatgagaaag ctcaaaaaga
    15121 attggcaagt catgtgaagt aaataatttc taatagaact ttctctccat gaggagaagg
    15181 ttcttttggg atttttaaag gaaataatat gaaaaaatat atttttatgc gtgttttgcg
    15241 gtcattggtt tcgattttct tagtaacgac tttgacctac acgattatct ataccttggt
    15301 tcctcgaaaa ttgattttca agcaggatcc taactataat aaaattgcga caacggctga
    15361 taaacgtgat aactatgaaa atactgtgtt tgagcgtatg ggctacattg agtattacga
    15421 tactaaagag ttgcaagaaa aggcaagtag catggattct tctgtaacag tagaagcaaa
    15481 tgcgaccaat aaagctattt atgaaaagta catcaatcaa ttaggtcatg gttggacttt
    15541 gggagaattt actgaaagtg gtcaattcta tgctactcgt gaaattccaa tttttgaacg
    15601 tgtttttcac ttctatgcta acttgattga cattgaccat acaaataaaa tccaagaccc
    15661 tgaaaatcca gacttgaaac gctaccttcg ttttgaaaat gatccagcta tcggatggtc
    15721 attggtcggt tcaggaacta aacataaata tctcttgtac tttaacagtc agttcccatt
    15781 tgttcatcaa aactttgtga acttgaattt aggtgactct tacccaacct atgctaatac
    15841 accagttctt caggttatta ctcaaggtca aggacaaacc aaaactgccc aagttcagtt
    15901 cccaacaggt aagaaaacgt cttctgtaaa tatttactca agaacctaca agtcacctag
    15961 tcaggctgac tctcgtgaag tagctagcta tgggaaagat gatccttata cagcgactga
    16021 aagtaattac caatatccat ctatgattgt cagctctgct attactggtt tgattggttt
    16081 ggttcttgcc tatgctcttg ccgtgccact tggttcagcc atggctcgtt tcaagaacac
    16141 ttggattgat agcctctcaa cagggacttt gaccttcttg cttgctcttc caacgattgc
    16201 cttggtttac atcgttcgat tgattggatc atctattgcc cttccagatt cattccctat
    16261 cttgggagct ggagattggc gttcttacgt tttaccagca gtcatccttg gtttgttggg
    16321 tgctcctgtt acagccattt ggattcgtcg ttacatgatt gacttgcaat ctcaagactt
    16381 tgttcgtttc gctcgtgcaa aaggtttgtc tgaaaaagaa atttcaaaca aacacatctt
    16441 taaaaatgcc atggttccgc tggtttcagg aattcctggt gccattattg gggttatcgg
    16501 tggtgcaacc cttactgaaa cagtcttcgc cttcccaggt atgggtaaaa tgttgattga
    16561 ctctgtaaaa gcatctaata actctatggt cgttggtctt gtcttcatct ttacatgtat
    16621 ttctatcttc tcacgtcttt tgggagatat ttggatgact attattgacc cacgtattaa
    16681 attgactgag aaaggaggca aataatgtct acaatcgata aagaaaaatt tcagtttgta
    16741 aaacgtgacg attttgcctc tgaaactatt gatgcgccag catattctta ctggaaatca
    16801 gtgtttaaac aatttatgaa gaaaaaatca actgtagtca tgttgggaat cttggtagcc
    16861 atcattttga taagtttcat ctacccaatg ttttctaagt ttgatttcaa tgatgtcagc
    16921 aaggtaaacg actttagtgt tcgttatatc aagccaaatg cggagcattg gttcggtact
    16981 gacagtaacg gtaaatcgct ctttgacggt gtctggttcg gagctcgtaa ctccatcctc
    17041 atttctgtga ttgcgacagt gattaacttg gttatcggtg tttttgtcgg tggtatttgg
    17101 ggtatttcaa aatcagttga ccgtgtcatg atggaagttt acaacgtcat ctcaaacatc
    17161 ccacctcttt tgattgttat tgtcttgact tactcaatcg gagctggatt ctggaatctg
    17221 atttttgcca tgagcgtaac aacatggatt ggtattgcct tcatgatccg tgtgcaaatc
    17281 ttgcgctatc gtgacttgga atacaacttg gcgtcacgta ctttgggaac accaaccttg
    17341 aagattgttg ccaaaaatat catgcctcaa ttggtatctg ttattgtgac aaccatgact
    17401 caaatgcttc caagctttat ctcatacgaa gccttcttgt ctttcttcgg tcttggatta
    17461 ccgattacag tgccaagttt gggtcgtttg atttcggatt attcacaaaa cgtaacaacc
    17521 aatgcttact tgttctggat tccattgaca acccttgtct tggtatcctt gtcccttttc
    17581 gtagttggtc aaaacttagc ggatgctagt gatccacgta cacatagata ggagtagaaa
    17641 tgacaaaaga aaaaaatgta attttgactg ctcgcgatat tgtcgtggaa tttgacgttc
    17701 gtgacaaagt attgacagcc attcgcggcg tttcccttga actagtcgaa ggagaagtat
    17761 tagccttggt aggtgagtca ggatcaggta aatctgtttt gacaaagacc ttcacaggta
    17821 tgctcgaaga aaatggtcgc attgcccaag gtagtattga ctaccgtagt caggacttga
    17881 cagctttatc ttctcacaag gattgggaac aaattcgtgg tgctaagatt gcgactatct
    17941 tccaggaccc aatgactagt ttggacccca ttaaaacaat tggtagtcag attacagaag
    18001 ttattgtaaa acaccaagga aaaacagcta aagaagcgaa agaattggcc attgactaca
    18061 tgaataaggt tggcattcca gacgcagata gacgttttaa tgaataccca ttccaatatt
    18121 ctggaggaat gcgtcaacgt atcgttattg ctattgccct tgcctgccga cctgatgtct
    18181 tgatctgtga tgagccaaca actgccttgg atgtaactat tcaagctcag attattgatt
    18241 tgctaaaatc tttacaaaac gagtatcatt tcacaacaat ctttattacc cacgaccttg
    18301 gtgtggtggc aagtattgcg gataaggtag cggttatgta tgcaggagaa atcgttgagt
    18361 atggaacggt tgaggaagtc ttctatgacc ctcgccatcc atatacatgg agtctcttgt
    18421 ctagcttgcc tcagcttgct gatgataaag gggatcttta ctcaatccca ggaacacctc
    18481 cgtcacttta tactgacctg aaaggggatg cttttgcctt gcgttctgac tacgcaatgc
    18541 agattgactt cgaacaaaaa gctcctcaat tctcagtatc agagacacat tgggctaaaa
    18601 cttggcttct tcatgaggat gctccaaaag tagaaaaacc agctgtgatt gcaaatctcc
    18661 atgataagat ccgtgaaaaa atgggatttg cccatctggc tgactaggag gaaggaaatg
    18721 tctgaaaaat tagtagaaat caaagattta gaaatttcct tcggtgaagg aagtaagaag
    18781 tttgtcgcgg ttaaaaatgc taacttcttt atcaacaagg gagaaacttt ctcgcttgta
    18841 ggtgagtccg gtagtgggaa aacaactatt ggtcgtgcta tcatcggtct aaatgataca
    18901 agtaatggag atatcatttt tgatggtcaa aagattaatg gtaagaaatc gcgtgaacaa
    18961 gctgcggaat tgattcgtcg aatccagatg attttccaag accctgccgc aagtttgaat
    19021 gaacgtgcga ctgttgatta tattatttct gaaggtcttt acaatcaccg tttatttaag
    19081 gatgaagaag aacgtaaaga gaaagttcaa aatattatcc gtgaagtagg tcttcttgct
    19141 gagcacttga ctcgttaccc tcatgaattc tcaggcggtc aacgtcaacg tatcggtatt
    19201 gcccgtgcct tggtcatgca accagacttt gttattgcag atgagccaat ttcagccttg
    19261 gacgtttctg tacgtgccca agtcttgaac ttgctcaaaa aattccaaaa agagctcggc
    19321 ctgacctatc tcttcatcgc ccatgacttg tcggttgttc gctttatttc agatcgtatc
    19381 gcagttattt acaagggtgt tattgtagag gttgcagaaa cagaagaatt gtttaacaat
    19441 ccaattcacc catatactca agccttgctt tcagcggtac caatcccaga tccaatcttg
    19501 gaacgtaaga aggtcttgaa ggtttacgac ccaagtcaac acgactatga gactgataag
    19561 ccatctatgg tagaaatccg tccaggtcac tatgtttggg cgaaccaagc cgaattggca
    19621 cgttatcaaa aaggactaaa ctagtaatgg ttttataatt tccatgtcaa tttttatgga
    19681 aattaggctc tttgtcaact gtagtgggtt gaagtcagct aagctcgaga aaggacaaat
    19741 ttcgtccttt cttttttgat gttcaaagcg ataaaaatcc gttttttgaa gttttcaaag
    19801 tttcgaaaac caaaggcatt gcgcttgata agtttgatga gattattggt cgcttccaat
    19861 ttggcgtttg aatagggtag ttgaagggcg ttgacgattt tctctttgtc cttttgtcct
    19921 ttagaaaggt tttaaagaca gtctgaaaaa taggatgaac ctgcttaaga ttgtcctcaa
    19981 taagtccgaa aaatttctct ggttccttat tctggaagtg aaaaagcaag agctgataga
    20041 gttgatagtg gtgtttcaag tcttccgaat aactcaaaag cttgtttaaa atctctttat
    20101 tggttaagtg catacgaaaa gtaggacgat aaaaacgttt atcactcagt ttacggctat
    20161 cctgttgaat gagcttccag tagtgcttga tagccttgtg ttcatgggat tttcgatgaa
    20221 actgattcat gatttggaca cgcacacgac tcatagcacg gctaagatgt tgtacaatgt
    20281 gaaagcgatc aagaacgatt ttagcattcg ggagtgaaac agtctgggag actgtttcag
    20341 cctgagccta gaaatttgaa agcgaagctg tttagccaag tcatagtaag ggctaaacat
    20401 atccatagta atgattttga cgcgacatcg gacggctcta tcatatttaa gaaagtgatc
    20461 tcggatgaca gcttgtgttc taccttcaag aacagtgatg atatcgagct tttcaaaatc
    20521 ttgcgcaata aagctcatct ccatctcccg attgaaacag tcactccccg gactgtttca
    20581 acgtcccaag acataatctt aggcagattt ctaaaattac actcaaagtg gaagtcattg
    20641 agctttcgaa tgacagttga agttgagatg gccagctgat gggcaatatc ggtcatagaa
    20701 ttcttttcaa ttaacttttg agcaattttt tggttgatga tacgagggat ttggtgattt
    20761 ttctttacca ggggagtctc agcgaccgct atttttgagc actgatagca cttaaagcga
    20821 cgctttctaa ggaggattct agtaggcata ccagttgtct caaggtaagg aatcttagac
    20881 ggtttttgaa agtcatattt cttcatttga cttccgcact cagggcaaga tggggcgtcg
    20941 tagtccagtt tggcgatgat ttctttgtgt gaatccctat tgacgacatc tataatttgg
    21001 gtatttgtgt ctttgatatc gagtagtttt gtgataaaat gtaattgtcc catatgaatc
    21061 tttctaatga gttgttttgt cgcttttcat tataggtcat atgggacttt ttttctacac
    21121 aaaataggct ccataatatc catagggggg gtacccacta caaatattat agagccgaaa
    21181 aaggctgaga tctggtgtct cagtctttta tttcttgcgt tttgcaaaat ctacaaatct
    21241 aaatttttct aacttatggc gactttcggt aaactgaaat tggcgtccgt cttggagata
    21301 gacttttgat ttaatcgaaa cgacataagg ttctttgcca atgtccatga gaatcttgtc
    21361 tcggtcgctt gagtggtcaa tggtgatttc cttctgagca taatcaataa ggagtttgag
    21421 gccattttct atataagaat agatagactg ctcagcaatt tggcgagtga gatttggaat
    21481 tagttccata tccagatagt ctgtatccag aacggatacc agatcatcca ccacacgctg
    21541 gcggaccacc ttccaaacca tccgaaactc tgggaaaccg gttatcagtg aggatttttt
    21601 atcaataata atcttgtcca gactgaccac gttggtttta gagcgcagtc caagttcttt
    21661 aactagttct tggtagctgg ttaggttgga tacagggaaa ttgacggttt cttctttgac
    21721 gacttgagaa ccttgccctc ttatcttttt gatcaatcct tcctcttgga ggagagacag
    21781 ggcctttcgg acggtatcac gactcacttg atattgctcc ataaggtcgt gctcgctagg
    21841 aaggaaatct ccgacagcgt aagtctcgtt ttgaatggtt tcttggattt gcttaaataa
    21901 ttgttggtat ttcttcattt tgcctgctta aataggtttt tagtctagtt taatgaacac
    21961 ctgtatctat tttaccatac aagtgctaga aaatttacaa aaaaacaccc tttttatacc
    22021 aaacaagttg cagacaagtt tggtatcgtt tacaatatac ttatcaaatc aaacttgctt
    22081 tgacaagtat aaggagaatc aaatgggaaa atttgaacaa gaagccaaag atctgcttca
    22141 ggcaattgga ggcaaagaaa atgtgactgc cgtcacccac tgtgcgacac ggatgcgctt
    22201 tgttttagga gatgataaga aggctaatgt taaagctatc gagtcaattc cagctgttaa
    22261 aggaactttt acaaatgcag gtcaatttca ggtaatcatt ggaaatgacg tgcccatctt
    22321 ttataatgat tttacagccg tttcaggtat tgagggtgtt tccaaagaag cagccaagtc
    22381 tgcagctaag agtaatcaaa acgtggtcca aggtgttatg accactctgg cggagatttt
    22441 tactccgatt attccagcct tgatagtcgg aggattgatc ctcggtttcc gtaatgtctt
    22501 ggaaggtgtc cattggtcga tgttggatgg caagaccatc acagaatcct ctcagttttg
    22561 ggcaggggtt aatcacttcc tctggttgcc tggtgaagct atcttccagt tcttaccagt
    22621 agggattact tggtctgttt ctcgtaagat gggaaccagc caaattttgg gaattgttct
    22681 cggaatctgt ttggtatcgc ctcagttgct caatgcctat gcggttgctt caacgccagc
    22741 agctgatatc gcggcaaact gggtttggaa ttttggttat tttactgtta atcgtattgg
    22801 ttaccaagcc caagttatcc cagccttgct tgcaggtttg agtctgtctt atcttgaaat
    22861 tttctggcgc aaacatatcc cagaagtcat ttccatgatt tttgtacctt ttttgtcatt
    22921 gattccagcc ttgattttgg ctcatactgt tttgggacca atcggttgga caattggaca
    22981 aggactttca tcagttgtct tggcaggttt aactggtcca gttaaatggc tcttcggtgc
    23041 aatttttggt gccctctacg ctccatttgt catcacaggt ctgcaccata tgaccaatgc
    23101 cattgataca caattgattg cggatgctgg tggcactgcc ctatggccaa tgattgctct
    23161 ttctaatatt gctcaaggct cagccgtgtt tgcctattat ttcatgcatc gccatgatga
    23221 gcgtgaggct caggtttcac ttcctgcaac catttcagcc tatctcggtg ttacagaacc
    23281 agctcttttt ggggttaacg taaaatatat ttatccattt gttgctggga tgactggttc
    23341 agcccttgca ggcatgttat ccgttacttt taatgtaact gcggcttcta ttggtatcgg
    23401 tggtttgcca ggtattctct ctattcaacc tcaatacatg ctgccatttg caggaactat
    23461 gctagttgcg attgttgttc caatgctctt gactttcttc tttcgcaagg ctggtctctt
    23521 cacaaaaata gagggcgata cgaacttgca ggcagaattc gttgctcaag aagaagcaga
    23581 atttgtgagc catgaaccag tagaacttac ttcggtagaa attatcagcc cactaactgg
    23641 ccaagtgaaa gaattgagtc aagcgacgga tcctgttttt gcatcaggtg tcatggggca
    23701 aggtctggtc attgaaccaa gccaaggtga gttgacctct ccagttaatg gaacagtgac
    23761 ggttcttttc cctaccaagc atgccatcgg cattgtctct gacgagggag ttgaattgct
    23821 catccacatc ggtatggata cagtaggtct tgatggcaaa ggttttgaaa gtcttgtagt
    23881 ccaaggagat cacgttatag ttggccagca actgattcgt tttgatatgg atgtcattaa
    23941 ggctgcaggt ctggtgacag aaactcctgt tatcatcacc aaccaagatg cttatacagc
    24001 gactattact ggaacttatc cgacaacgat ccaagctgga gcatctctca tggtcgctac
    24061 acgaatctaa tactcttcga aaatctcttc aaaccacgtc agctctatct gcaacctcaa
    24121 aatagtgttt tgagcaacct gtggctagct tcctagtttg ttctttgatt ttcattgagt
    24181 ataattgaaa tagcacaaat agggacggaa gactgcttcc tcccttttat cagaaaagga
    24241 gaaacagatg acacttgata aaggaaaagt agtctatcaa atctatccaa aatcttacaa
    24301 agacaccact gaaaacggta ttggggattt ccgtgggatt atcgaaaaaa ttccctattt
    24361 agccaaacta ggtgtggata tggtctggct caatccattc tatccaagcc ctcaacggga
    24421 taatggttac gatatttcag attatatggc agtggatcct ctttttggtg atatggctga
    24481 ttttgaggaa atagtgtgtg tcggtaaaga gcacaagatt gactttatgc tggacatggt
    24541 acttaatcat tgttcgacag agcatgaatg gtttcagaaa gccctagctg gtgataagta
    24601 ttatcaagac tttttcttca tccaagacca accaacagat tggcagtcta agtttggtgg
    24661 ttctgcatgg gcgcctttcg gggatactgg gaaatactat cttcacctat ttgatgagac
    24721 ccaggctgac cttaactggc gcaatcccaa tgtccgtaag gagcttttca aggttgttaa
    24781 tttctggcgc gacaagggtg tcaaaggttt tcgatttgat gtgatcaatt tgatcggtaa
    24841 ggacgaggtt tcagtggact gtcctgaaaa tgaagggaag ccagcttata cagacaaacc
    24901 catcgttcat aactatttgc gtatgatgaa ccaagccact tttggatctg acgatagctt
    24961 tatgacagtt ggggaaatgt cttctaccac catggaaaac tgtgtccttt attcgtcgcc
    25021 tgatcgtcag gaattatcca tgacctttaa ttttcatcac ctcaaggtgg actataagga
    25081 tggacaaaag tggaccttgg ctccctttga ttttgaagag ttgaagagtc tttaccatag
    25141 ttggggtaag gaaatgagtg ataaagacgg ttggagtgcc ctcttttgga acaatcacga
    25201 tcaaccacgt gccttgaacc gttttgtcga tattcaaaac ttccgcaagg aaggagccac
    25261 catgctagca gccagcattc acctgtcacg tgggacacct tatatctaca tgggtgagga
    25321 aatcgggatg attgacccag actatgattc tatggctgat tatgtggatg tcgaatcgct
    25381 taatgcctat cagatgctct tagaagaagg aaagagtcag caagaagcct tccagattat
    25441 tcaagctaag tcgcgtgata attcacgtat tcccatgcag tgggatactt ctgaaaatgc
    25501 aggattttca acaggcactc catggttgaa agttggtaaa tcctataaag atatcaatgt
    25561 agaaaatgaa atcaaaggtc tgattttcac cttctatcaa gacttgattc gactgcgtaa
    25621 ggaaatgcca atcatttcag aaggaaatta caaaccagcc tttgaagata gcaaacaagt
    25681 ctacgctttt gaacgccagt ttgaggatca aaagttacta gtgctcaata acttttatgc
    25741 caaagaagtg gaaatcgact tgccagcagt ctaccaaaat ggacagattt tgatttcaaa
    25801 ctatgaagat gcagaagtgt ctgagaaaat tctactcaaa ccctatcaaa cactggctat
    25861 ttatgtaaat taatactctt cgaaaatctc ttcaaaccgt gtcagtttta tctgtaacct
    25921 caaagcggtg ctttgagcaa cctgtggcta gcttcctagt ttgctctttg attttcattg
    25981 agtataaact tagcaaggag acctcttttt cagaaaagca gggttctttt tgctgtctat
    26041 tccatccttg aaagtgtaga acaaactggt cagggtggct agtttgtggt ataatgaaag
    26101 ggattcaaaa agaaacagga gaaaaatgat ggatttactt ttagcaattg tattgattgt
    26161 gctagctttt ctaggaggag ctcttggagg aatgtacttg gttcgtaagc aaatcgaaaa
    26221 agaatttgct gacaacccac gtttgaatgc tgaagcagtt cgtactcttt tgagtgcaaa
    26281 tggtcaaaaa ccaagcgaag ctaaggtaca acaagtttac caccaaatca tccgccaaca
    26341 aaaggcagcc cttgctaaca ataaaaagaa aaaataaata aggaaaagtc tgggatgaaa
    26401 gttccagact tctcactatg ttggttatgg tttagggatg agactttttc cttgcattct
    26461 attgatattt gattatgatt aagagagagg tagttgacat tctatcaagc tgtcggtcag
    26521 ttcgttcagt acaaggaatc ataatgaacg atcaatcaga aaaaagacta gaaagaagac
    26581 tgtatggata atcgaccaat tggttttttg gattcgggtg tcgggggctt gaccgttgtg
    26641 cgcgagctca tgcgccagct tccccatgaa gaaatcgtct atattggaga ttcggcgcgg
    26701 gcgccctatg gcccccgtcc tgctgagcaa attcgtgaat atacttggca gctggtcaac
    26761 tttctcttga ccaaggatgt caaaatgatt gtcattgctt gtaacactgc gactgcggtc
    26821 gtctgggaag aaatcaaggc tcaactagat attcctgtct tgggtgtaat tttgccagga
    26881 gcttcggcag ccatcaagtc cagtcaaggt gggaaaatcg gagtgattgg aacgcccatg
    26941 acggtacaat cagacatata ccgtcagaaa atccatgatc tggatcccga cttacaggtg
    27001 gagagcttgg cctgtcccaa gtttgctccc ttggttgagt caggtgccct gtcaaccagt
    27061 gttaccaaga aggtggtcta tgaaaccctg cgtcccttgg ttggaaaggt ggatagcctg
    27121 attttgggct gtactcatta tccactcctt cgccctatta tccaaaatgt gatggggcca
    27181 aaggttcagc tcatcgatag tggggcagag tgcgtacggg atatctcagt cttactcaat
    27241 tattttgaaa tcaatcgtgg tcgcgatgct ggaccactcc atcaccgttt ttacacaaca
    27301 gccagtagcc aaagttttgc acaaattggt gaagaatggc tggaaaaaga gattcatgtg
    27361 gagcatgtag aattatgaca aataaaattt atgaatataa ggatgaccag gactggtatg
    27421 ttgggtctta tagtattttt ggtggcgtta acagtttgag cgactataag acagattttc
    27481 ctctgtttga attctccaaa atatttggag atgaagagta tggtttcccg ctttcagtta
    27541 ctgttttacg ctatggttct acctaccgtt tgttctcctt tgtggtagac atgcttaatc
    27601 aagaaatggg acgaaacttg gaagttattc aacgtcatgg ggccctgctc ttggttgaaa
    27661 atgggcaact cttgtatgta gaattgccta aagaaggggt caatgttcat gatttctttg
    27721 agacaagcaa ggtcagagaa accttgttga ttgcgactcg taacgaaggt aaaaccaagg
    27781 aattccgagc tatctttgat aagttaggct acgatgtgga aaatcttaat gactaccctg
    27841 acctgcctga agtagcagaa acaggtatga cctttgaaga aaatgcccgc cttaaggcag
    27901 aaaccatttc tcaattaacg ggcaagatgg ttttggcaga tgattctggt ctcaaagtcg
    27961 atgtccttgg tggcttacca ggcgtctggt cagctcgttt cgcaggtgtg ggagcaactg
    28021 accgtgaaaa taatgccaaa ctcttgcacg aattggccat ggtctttgaa ctcaaggacc
    28081 gctcggctca gttccacaca accctagtcg tagccagccc aaataaggaa agtttagttg
    28141 ttgaagcaga ctggtcaggt tatattaact ttgaacctaa gggtgaaaat ggctttggct
    28201 atgatcccct cttccttgta ggagagacag gtgagtcatc agctgaatta accctggaag
    28261 aaaaaaatag tcaatctcac cgtgccttag ccgttaagaa acttttggag gtatttccat
    28321 catggcaaag caaaccatca ttgtaatgag cgattcccat ggcgatagct tgattgtgga
    28381 agaagtccgt gatcgctatg tgggcaaagt cgatgccgtt tttcataacg gcgattctga
    28441 actacgtccg gattctccac tttgggaggg catccgcgtt gttaaaggga acatggactt
    28501 ctacgccggc tacccagaac gtctggtgac tgagcttggt tcgaccaaga ttatccaaac
    28561 tcatggtcac ttgtttgaca tcaatttcaa ctttcaaaag ttggactact gggctcagga
    28621 ggaagaggcc gctatctgcc tctatggtca cttgcatgtg ccaagtgctt ggttggaagg
    28681 caagatcctc tttctaaatc caggttctat cagtcaacca cgaggtacca tcagagaatg
    28741 tctctatgct cgtgtggaga ttgatgatag ttacttcaaa gtggactttt tgacacgaga
    28801 tcacgaggtg tatccaggtt tgtccaagga gtttagccga tgattgccaa ggagtttgag
    28861 actttcttgt tggggcagga ggaaactttt ttgacccctg ctaaaaatct agctgtgttg
    28921 attgataccc acaatgcgga tcatgcgacc ctcttgctca gtcagatgac ctatacccgt
    28981 gttcccgttg tgacagatga aaaacagttt gttgggacga ttggactcag agatattatg
    29041 gcttatcaga tggagcatga cttgagccaa gaaatcatgg cggatacgga tatcgttcat
    29101 atgacaaaaa cggacgtagc ggttgtttcg cctgatttca ccattacgga ggtcttgcac
    29161 aagctagtag atgagtcctt cttaccggtt gtggatgcag agggtatttt ccaagggatt
    29221 attacgcgca agtccatcct caaggccgtt aatgccctct tgcatgactt tagtaaggaa
    29281 tatgagattc gatgccaatg agagacagga tttcagcctt tttagaggaa aagcagggct
    29341 tgtctgtcaa ttccaagcag tcctataagt atgatttgga gcaattttta gacatggtag
    29401 gtgagcggat ttctgagacc agtctcaaga tttaccaagc ccagctagcc aatctaaaaa
    29461 tcagcgccca gaagcgaaag atttcggcct gtaaccaatt tctatacttt ctctatcaaa
    29521 aaggagaggt ggacagcttt taccgcttgg aattagccaa acaagctgaa aagaagacgg
    29581 aaaagccaga gattctatac ctagactctt tttggcagga aagcgaccat ccagagggcc
    29641 gcttgctagc gctcttaatc ctagaaatgg ggcttttgcc cagtgagatt ttagccatca
    29701 aggttgcgga catcaatctg gattttcagg tgttgcgaat cagcaaggct tcccaacaga
    29761 ggattgtcac tattcccacg gccttgcttt cagaattgga acccttgatg gggcagacct
    29821 atctttttga aagaggagag aaaccctatt ctcgtcagtg ggcctttcgt cagttagaat
    29881 cttttgtcaa ggagaaaggt tttccatcct tatcagctca agtcttacgt gaacagttta
    29941 ttctaagaca aatagaaaac aaggtcgatt tgtacgaaat tgcaaaaaaa ttaggattaa
    30001 aaacagtcct gaccttagaa aaatatagat aatggatatt aaattaaaag attttgaagg
    30061 acccctggac ttgctcttgc atctggtttc taagtaccag atggatatct acgatgtgcc
    30121 cattacggaa gtcatcgaac agtatctagc ctatgtctca accctgcagg ccatgcgtct
    30181 ggaagtgacg ggtgagtaca tggtcatggc tagtcagctc atgctgatta agagtcgtaa
    30241 actccttccg aaggtagcag aagtgacaga cttgggggat gacctggagc aggacctcct
    30301 ctctcaaatc gaagaatatc gcaagttcaa gctcttgggt gagcacttgg aagccaagca
    30361 ccaagaacgg gcccagtatt attccaaagc gccgacagag ttgatttacg aagatgcgga
    30421 gcttgtgcat gacaagacga ccattgacct ctttttggct ttttcaaata tcctagccaa
    30481 gaaaaaagag gagtttgcac aaaatcacac gacgatcttg cgggatgagt ataagattga
    30541 ggacatgatg attatcgtga aagagtcctt gattggacga gatcaattgc gcttgcagga
    30601 tttgttcaag gaagcccaga atgtccaaga ggtcatcacc ctctttttgg caaccctaga
    30661 gttaatcaaa acccaggagc tgatcctcgt gcaagaggag agttttggag atatctatct
    30721 catggaaaag aaggaagaaa gtcaagtgcc tcaaagctag acttgataga gaggaaagat
    30781 gagtacttta gcaaaaatag aagcgctctt gtttgtagcg ggtgaagatg ggattcgggt
    30841 ccgccagtta gctgaactcc tctctctgcc accgacaggc atccagcaaa gtttaggaaa
    30901 attagcccag aagtatgaaa aggacccaga ttccagtttg gctttgattg agacaagtgg
    30961 tgcttataga ttggtgacca agcctcaatt tgcagaaatt ttgaaggaat actctaaggc
    31021 gcctatcaac cagagcttgt ctcgggctgc ccttgagacc ttgtccatta ttgcctacaa
    31081 acagccgatt acgcggatag aaattgatgc catccgtggg gttaactcga gtggagcctt
    31141 ggcaaagttg caggcttttg acctgataaa ggaagacggg aaaaaggaag tattggggcg
    31201 ccccaacctc tatgtgacta cggattattt cctagattac atggggataa accatttaga
    31261 agaattacca gtgattgatg agcttgagat tcaagcccaa gaaagccaat tatttggtga
    31321 aaggatagaa gaagatgaga atcaataagt atattgccca cgcaggtgtg gccagtagga
    31381 gaaaagcaga agagctgatt aagcaaggct tggtgacggt taacggccaa gtggtgcgtg
    31441 aactagcaac cactatcaag tcaggcgaca aggtcgaagt tgaaggtcaa cctatctaca
    31501 acgaagaaaa ggtctactat ctgcttaaca aaccacgcgg tgtgatttcc agtgtgacag
    31561 atgataaggg tcgcaagacg gttgtcgacc tcttgcccaa tgtcaaagaa cgtatttacc
    31621 ctgtgggtcg tttggactgg gatacatcag gtgtcttgat tttgaccaat gatggggact
    31681 ttacagacga gatgattcac cctcgtaatg agattgacaa ggtttatgtc gcgcgtgtta
    31741 aaggtgtggc caataaggac aatctccgcc ccttgacccg tggtcttgag attgatggta
    31801 agaaaaccaa gccagctgtt tatgaaattc tcaaagtgga cccagtcaaa aatcgctctg
    31861 tggtgcagtt gaccatccat gaagggcgta accatcaggt taaaaagatg tttgaagctg
    31921 ttggtctcca agtagataag ttgtctcgga ctcgtttcgg acacctagac ttgacaggac
    31981 tccgtccagg agaatcccgt cgtcttaata aaaaagaaat cagccaacta cacaccatgg
    32041 ctgtaactaa gaaataatga aacgaatttt aatagcgcct gtgcgctttt accaacgttt
    32101 tatctcacca gtctttccac cctcttgtcg ctttgagctg acttgttcca actacatgat
    32161 tcaggctatt gaaaaacatg gttttaaggg ggtattgatg ggcttggctc ggattttacg
    32221 ttgtcatccc tggtcgaaaa caggtaagga ccccgttcca gaccactttt cccttaaacg
    32281 aaatcaagaa ggggaatgag gtggggtaaa tagatttcaa aatgataaaa acgcatccta
    32341 tcaggtttga gtgaatttga taggatgcgt tttagaatgt caaaatttta tactcttcga
    32401 aaatcccttc aaaccgcgtc tgctttcatc tgcaacctca aaacagtgtt ttgagcaacc
    32461 tgcggctagt ttcctagttt gctctttgat tttcattgag tattaaattg agtttgaagt
    32521 ggcttatttc aaagcttttt gtatgtcttc aatcatgagt tttgttgatt caagtccgcc
    32581 tccgcttaga taccagaggt ctggtgttag ttggataatc ttaccatttt tagcagcagg
    32641 tgtttcagcg ataagggcat tttctaggac accgtcgttg ctagagttgt ccccaccgat
    32701 ggcaagggta cggttgatga caaagaggat gtcagggttg atttctttga cactttcaaa
    32761 gctgacttct tgtccgtggc gtgagtcttc aaatgttgta tcagttggtt tgaatttcaa
    32821 ggtttggtac aagaaagaga aacgagattt ggcaccaaag gctgccattt ttccttcatt
    32881 aaggaggatc gcaagggcct ttttgtcaga gctttcattt ttagtagcga cttcttggat
    32941 gctcttgtct agcttggcca attcttcctt ggctttctgt gtaccagttt cgccgaaggc
    33001 acttgctaag gattcgatat tagccttggt agaagtccag tagtcgtcct tgcttgcttg
    33061 gaagagaacg gttggggcga tttctttgaa tttgtctacg aatttttgtg tacgtggtga
    33121 agcgataatc aaatcaggct caagggcggc gatagcttct aaatcaggtt ctttcataga
    33181 accaacattt ttgacagttc ccactaggtc ttttagataa gtcggaacag tttttgtagg
    33241 cattccgacg atattttttt caaatcctaa agcgcgaata gtatccgcag cgccgaggtc
    33301 aaaggtcaca atcttttcag gaactttgga aagtttgacc tcgtccagtg aacttttaat
    33361 ggttacctct gttggagcag agctactggt ctctgtctga ctagtgcttg agtttgtact
    33421 acatgcacca agtaggagca agaagctggc cactagggca gtgaaataaa gtttaaggga
    33481 tgttttcata atttctcctt tttaaaatgt gataacgatt tagggagtct cttgatctta
    33541 ttgattaaga gactgaaggt tctctaactt gagcttttat gttactagct atagatacag
    33601 atctttttgt cattgatatc agctagcgtg atgggaatct cataaagttg gctgagcagg
    33661 tcagcctgca tgatttgatc ggtgcttccc ttgctaaaga cctgaccgtc cttgaaggcg
    33721 acaatttcat ctgcatactg actggccatg ttgatatcgt ggaggacgat gataatggtc
    33781 ttgccgagtt cctccaccag tcgtcgaaga atttgcatca tgctgacgct ttgcttgata
    33841 tcgagattgt tgagtggttc gtccagcaag ataaagtccg tatcctgggc cagtaccata
    33901 gcgataaaga cgcgctggag ttgcccccct gacaggctat tgatgtagcg gtcttttaag
    33961 ttggtcagtt ccaaatagtt cagagtttct cggatttttt cccagtcttc tgatctaagt
    34021 cgacctcggc tgtagggaaa acgtccaaaa ctgaccagtt cttcaacagt caatttggct
    34081 tggtaattga ttttctgttt taggatggtt agttcttggg ccagttcttg cgaattccag
    34141 ctctcgattt cacgtccttt gatactgaga actccctgat ctttcttggt tagtctgctc
    34201 atgatggaga ggagagtcga ttttccagca ccatttggac caataaaggc tgtcagtttt
    34261 tgaggactga cttcaagcga aatgccttgc aaaatatcct gtttttgaat ggatttgtca
    34321 atgttttcca gtttcactga cgagccctcc tatatagtaa gataaagaat aagaagccac
    34381 ccacactctc aatgatcata ctgatacgaa tttccagtgc aaagactcgt tcaatcaagg
    34441 cttgccccaa ggttaagcta ataaatccaa ccagaatggc cactataaag agtaacttgt
    34501 gctgatagtc tttgacaatc aggtaggtga ggttggccag cataaagccg aagaaggcca
    34561 taggtcctac caaggcagtg gccgttgagg tcaaaagcac gattccccag aggagctctt
    34621 tctgttcttt ttcaacatcg agtcccaata tctgagccgt ttctctttgc aggtgcaaga
    34681 catctagaac gactgctttt cgaaagaaaa agattgtcaa agcgaggatg atcagagaac
    34741 cgatggctag gatggaagtg ttaagatgtt gaaaggaggc aaaaagacta ttttgcagtt
    34801 tatcgtattc gtttggatcc attaggactt gaaggaaggt gctgatattt cgaaagagac
    34861 ttctgagcgc tagacagatc agcaggatga agaccaggtc ttgcttcatc agtgtcttca
    34921 agtaaccttg taaggcgaga aagaagaggg actggacaag aagtaagact aggaattcta
    34981 agatagggga tttgccaagt tgaagaaact tgctttcaaa aaccagtagt agggtttgta
    35041 gtaggacgta gaaggattca attcccaaaa tactaggcgt caggaagcga ttttccgtca
    35101 gggtttgaaa actaatggtc gaaatcccag tcgcgatggc taccaagaga taaacgatga
    35161 tcttttggga acgcaacttc caagcaaagg ctgacaagtg agtgatgggc caaaagtaga
    35221 gaagacaagc tccgatggca agaataatga gaatccagaa gagcttggta tgtttgcttt
    35281 tagtctgcat cttttcgtcc ccctctccag agaagtagga taaagacgag actaccgatg
    35341 attcctagca agagactgac agacaactca tagggcctaa tcagaactcg ggataggata
    35401 tcgcaagcca gaactagatt ggcaccaacc agtgcgacca tgagtttggt ttgacttaga
    35461 ttatctccat agcgcttgcg aacaagattg ggaacgataa ctccgagaaa tggtaggcca
    35521 cccacggtaa tcatggtgac gcttgtcgtt agcgccacca gaaagagggc cagtttttca
    35581 agtagggagt aggaaatccc caaactctcg ctggtttctt tccctagatt catgatggtg
    35641 aaggtttggg ataatttcca aacggttatc aggatgatga ggcctaagaa gagccactca
    35701 tactgatggg tctgaatcat ggagaaggag ccctgggtcc aggcagtcat actctgaacc
    35761 agattgaaac gataggcgat aacttctgtg actgagccga taatcccgct atagatgatc
    35821 ccaatcagag gcaacatcca cctttccttt acagtaaaaa tggtcataaa ggctaggaag
    35881 aagagggtga atacgatgga tgaaacaaaa gcgaagagca tcttttgggt cagactagcc
    35941 gatggaaaga caaaaaggct cagcaccatt cccagtttgg cggcttcagt cgttccaact
    36001 gtactcggtg cagcaaactg attttgggta atagtctgca tgagaaggcc tgccatactc
    36061 atactagagg cagtcaggag aatactgata gttcttggga gacgggactc ttgaaagagg
    36121 agccaggtct gctggtcgaa atcaaatagc tttccccatg aaaaatcact ggtcccaatg
    36181 ctaatagaga gaaagactag gagtagaagt aagccaatta aataatgaga aagtttcata
    36241 ccccgtcctt tcatgtagat ttggtatcga aagatatctg cggatataaa tgtaacatta
    36301 tttttctaat ctgtcaataa aatttctgac aatttaataa atacaacaag gagagagcaa
    36361 caagactttc tcctttgtta tcctattcta aaatgttttt accttaatct gataaaataa
    36421 tatcttcgag ggagtagcta gccgtccaat caagatattg tttagctttt gaagcatctg
    36481 ctaggacact ggctgggtca ctagcacgtc gagcaacaat ctcgtgtggg attttttaat
    36541 ttagtaattc ttcagcagtt ttaaagattt ctttgatagt atagcctttt ttagttccta
    36601 agttaaagat ttgagaagaa ctgtcttctt gaaataggta gttcattcct ttaacatgag
    36661 cctatgcaag gtccaagaca taaatgtaat ctcgaataca tgaaccgtca cgtgtatcgt
    36721 agtcatctcc aaatattttt aagctatcat tttgtcccaa tgcggtcttg ttgatatttg
    36781 gaatgatgtg agttggattt ttcacacgca gaccgtttga agcatccatt tcagccccag
    36841 caacattaaa gtaacggaaa ataacatatt tccagtcgta gcgattggcc atccagtaaa
    36901 tcattcgttc gcccatcagt tttgtctctg cataagggtt gacagggtcg agcagggtat
    36961 cttcagtcac cggcttgtca atacagttat ttccatagag agaagcagtc gaagagaaca
    37021 tgattttttg aatgccaact tcagataaga ctttgagaac ttggttcata ccagcaacgt
    37081 tggcagtgaa gtatttactt ggattttcaa tactttcgct cacaacaatt tcacctgcac
    37141 aatggaggac agcatcaatc tgattttctt ctaagtaagc ctttaaggcg ctagcatcat
    37201 aaacatccag ttctttaaag ctagcacgac tacccacagc cgctcgattt cctgtggaga
    37261 gattatcgag gacatgcacc ttataaccag catttaaaag agctttaacg gtatgggagc
    37321 caatgtagct agtcccacct gtaacaagaa ttgttttggt catgattttt tccttttcta
    37381 gttttgtact atagtaaatt gaaataagat atgaacaact ctattaggaa agtcaaatta
    37441 atttctagaa atgttttagc agctacagcg tactattcca aactcaacca actatatatt
    37501 taaagaaaga agaaagggaa gtcaactatt ttctgtaaat ttcatgaaaa acctaaacaa
    37561 ctttgatggt gtattgtttt tttgtttgga aataaatatt cgagttaatt ttggaatgtt
    37621 tactagattt tgcactacat gcaagagaat actatccttt tctaagatag agcaggacta
    37681 agatactaac ttagaaatcc accttcccac tatccttctc ctataaaaag tggtaaaatg
    37741 gatgaaagaa agaaggaatt gaaatgacaa cattattttc aaaaattaaa gaagtaacag
    37801 aacttgctgc agtctcaggt catgaagcgc ctgtccgtgc ttatcttcgt gaaaagttga
    37861 caccgcatgt ggatgaagtg gtgacagatg gcttgggtgg tatttttggt atcaaacatt
    37921 cagaagctgt ggatgcaccg cgcgtcttgg tcgcttctca tatggacgaa gttggtttta
    37981 tggtcagcga aatcaagcca gatggtacct tccgtgtcgt agaaatcggt ggctggaacc
    38041 ccatggtggt tagcagccaa cgtttcaaac tcttgactcg tgatggtcat gaaattcctg
    38101 tgatttcagg ttctgttcct ccgcatttga ctcgtggaaa ggggggacca accatgccag
    38161 ccattgccga tatcgttttt gatggtggtt ttgcggacaa ggctgaggca gaaagttttg
    38221 gcatccgtcc tggtgatacc atcgtaccag atagttctgc aattttgaca gccaatgaaa
    38281 aaaatatcat ctcaaaagct tgggataacc gctacggtgt cctcatggta agcgagctag
    38341 ctgaagcttt atcaggtcaa aaactcggca atgaactcta tctgggttct aacgtccaag
    38401 aagaagttgg tctgcgtggc gctcatacct ctacaaccaa gtttgaccca gaagtcttcc
    38461 tcgcagttga ttgctcacca gcaggtgatg tctacggtgg tcaaggcaaa attggagatg
    38521 gaaccttgat tcgtttctat gatccaggtc acttgcttct cccagggatg aaggatttcc
    38581 ttttgacaac ggctgaagaa gctggtatca agtaccaata ctactgtggt aaaggcggaa
    38641 cagatgcagg tgcagctcat ctgaaaaatg gtggtgtccc atcaacaact atcggtgtct
    38701 gcgctcgtta tatccattct caccaaaccc tctatgcaat ggatgacttc ctagaagcgc
    38761 aagctttctt acaagccttg gtgaagaaat tggatcgttc aacggttgat ttgattaaac
    38821 attattaaac cataagggag gtggtaggga tggcagaacc aaacctagaa agccttataa
    38881 aagatcttta caatcatgct cgacatgatt tgagtgaaga tttagttgct gctctcctag
    38941 agactactaa aaaactgcct actacaaatg agcaattgca ggcagttcgt ctctcaagcc
    39001 tggtcaatcg tgaattgctc ctaaatccca aacatccagc acctgagttg ctcaacttgg
    39061 ctcgctttgt caaaagagaa gaagccaagt acagaggaac tgcgacttct gcgcttatgt
    39121 atgaggaact ttttaaaatg ctttgattcc attgtggatc aaagcatttt ttataagacc
    39181 tatctcgtga gcgttcaaat agtagtaaca aggaatcatg agaagttagc cgagaccata
    39241 gtagtgaaga aacttctgta atggaagtgg agtgaagtga ggaatcgtca aacggtaggg
    39301 gataggctgt ttgaagggtg ctacgaaaaa gatagggtaa ggcgaaatta taacgggatg
    39361 aaataagata tgaacaaatt gattgaggaa attaaatgaa tttatagaaa tcttttagca
    39421 gtcatggtgt cctattccag ttgcaaaatg ctataaatac gaacaaattg attgggaaat
    39481 tcaaatcgct ttctatcaat gttttagaag atacagtgcc ctagtccaac ttcaatatac
    39541 tataaaagaa cagatggaag caattatgat ttacttctat ctttaaaata aatggtctga
    39601 taggaaaaat gtgtcccctc ataaaaaata ggatagtacg gatggaaggc ttttgtagac
    39661 tggaggacga aggagtttag aaactctcgt taaaagttag ttgagccgga gtatggtggt
    39721 gtggataatt atcaaaaatt atttctaaat cgaaatagtt gacagttaga acgatgagtg
    39781 ttacaattgt tttattcatt tcgatatcga aataaattgg aggtgttatg aaagatagtc
    39841 atttgctagc ccatcatatt cgtttgttga atgggcggat ttttcaaaag ttactgagtc
    39901 aagatcctga ggctctttat aggggtgaac agggcaagat tttagcggtt ttatggaata
    39961 gtgaaactgg ctgcgcaact gcgacagata tcgcgcttgc gactgggctt gcgaataata
    40021 cgctgacgac tatgataaaa aagctagagg aacaaaagct tgtaattgtt agtccgtgtg
    40081 gaaaagacaa gcgtaagaag tatttagttt taacggagtt aggcaagtcc cagaaagaag
    40141 tggggcatcg tgtcagtcag aaattggata ctatctttta caaaggattt tcagaggaag
    40201 aaattcacca atttgaaggt tttcaagaaa gaattttggc gaatctgaaa gagaagggaa
    40261 atgaggttta gaatatgtca caacaagtga aaaatgctca caacctatac atccatgcca
    40321 ttcaagacgg acgagttgct gaggctcaag cccagtctgt aggggatacc tatatccaac
    40381 attcgacagg tgttcctgac gggaaagaag gatttgcagc cttctttgca gatttctttg
    40441 agcgtcatcc agagcgtcag attaagattg tccgcaccat tgaggatggc aatctggtct
    40501 ttgttcatgt tcatcaatat ctgaatggtg gagaagctca atgggtgacg acggatactt
    40561 tccgtgcgga tgagaatggt tgtattgttg agcattggga tgtcattgac tactatcgaa
    40621 cacctgaaaa tgaccaacta gatcaaattt ttggagattt tgaaatcaag gatttggata
    40681 agaaagcaga aaataaaaag ctggttcgcc gtttcttgac agaaattttc caaaatgggg
    40741 agctagagca gtggagtgat tatgtggcag acgatttgat tcagcataat catgagattg
    40801 gacaaggaag tgctgcttat aaaaactatg tggctgaata tagtgtcact tttgactttg
    40861 ttttccaact cttaggacaa ggaaactatg tggttagcta tggtcagact cagattgatg
    40921 gcgttgctta tgcccagtac gatatcttcc gtttagagaa cgggaaaatt gtggagcatt
    40981 gggataataa ggaagtcatg cctaaggtag aagacttgac taatcgaggg aagttttaaa
    41041 ttgaggacaa agaatgattg aatacaaaaa tgtagcactg cgctacacag aaaaggatgt
    41101 cttgagagat gtcaacttac agattgagga tggggaattt atggttttag tagggccttc
    41161 tgggtcaggt aagacgacca tgctcaagat gattaaccgt cttttggaac caactgatgg
    41221 aaatatttat atggatggga agcgcatcaa agactatgat gagcgtgaac ttcgtctttc
    41281 tactggttat gttttacagg ctattgctct ttttccaaat ctaacagttg cggaaaatat
    41341 tgctctcatt cctgaaatga aggggtggag caaggaagaa attacgaaga aaacagaaga
    41401 gcttttggct aaggttggtt taccagtagc cgagtatggg catcgcttac ctagtgaatt
    41461 atctggtgga gaacagcaac gggtcggtat tgtccgagct atgattggtc agcccaagat
    41521 tttcctcatg gatgaaccct tttcggcctt ggatgctatt tcgagaaaac agttgcaggt
    41581 tctgacaaaa gaattgcata aagagtttgg gatgacaacg atttttgtaa cccatgatac
    41641 ggatgaagcc ttgaagttgg cggaccgtat tgctgtcttg caggatggag aaattcgcca
    41701 ggtagcgaat cccgagacaa ttttaaaagc gcctgcaaca gactttgtag cagacttgtt
    41761 tggaggtagt gttcatgact aatttaattg caacttttca ggatcgtttt agtgattggt
    41821 tgacagctct atctcaacat ttgcagttgt cacttttgac cttgttacta gctattttgc
    41881 ttgcgattcc cttggctgtt tttcttcgct atcatgagaa gctggccgac tgggtcttgc
    41941 agattgcagg tattttccag accatcccgt ctctggcctt gttgggactc tttattccct
    42001 tgatgggaat tgggacttta ccggcattga cagctctagt gatttatgcg atttttccga
    42061 ttttacaaaa tactattact gggctgaagg gaattgatcc gagtcttcaa gaggctggga
    42121 ttgcctttgg gatgaccaga tgggagcgtc tcaagaagtt tgaaattcca cttgccatgc
    42181 ctgttatcat gtctggaatt cggacggcag cggtcttgat tatcggtacg gctaccttgg
    42241 cgaccttgat tggggcaggg ggactgggtt cctttatcct gttgggaatt gaccgtaata
    42301 atgccagttt gattttgatt ggggcccttt cttctgcagt gctagctatt gcctttaact
    42361 tcctactaaa agtaatggaa aaagcgaaat tgcggacgat tttctctggt tttgccttgg
    42421 tggctttatt actgggtctg tcttatagtc cagccctttt ggctcaaaaa gagaaggaaa
    42481 acttggttat tgctgggaaa ataggcccag aaccagaaat tttggctaat atgtataaat
    42541 tgctgattga agaaaatacc agcatgactg cgactgttaa accgaatttt gggacgacaa
    42601 gtttccttta tgaagctctg aaaaaaggtg atattgacat ttatcctgaa tttaccggta
    42661 cggtgactga aagtttgctt caaccatcac ccaaggtgag tcatgagcca gagcaggttt
    42721 atcaagtggc gcgtgatggt attgccaagc aggatcatct agcctatctc aaacctatgt
    42781 cttatcaaaa tacctatgct gtagctgttc cgaaaaagat tgctcaagaa tatggcttga
    42841 agaccatttc ggatttgaaa aaagtggaag ggcagctgaa ggcaggcttt acgcttgagt
    42901 ttaacgaccg tgaagatgga aataagggct tgcaatcaat gtatggtctc aatctcaatg
    42961 tggcgactat gcagccagct cttcgttatc aggcaattca gtcaggggat attcaaatca
    43021 cggatgccta ttcgactgat gcggaattgg agcgttatga tttacaggtc ttggaagatg
    43081 acaagcaact cttcccacct tatcaagggg ctccactcat gaaagaagct cttctcaaga
    43141 aacacccaga gttggaaaga gttcttaata cattggctgg taagattaca gaaagccaga
    43201 tgagccagct caactaccaa gtcggtgttg aaggcaagtc agcaaagcaa gtagccaagg
    43261 agtttctcca agaacaaggt ttgttgaaga aatgatggga cagagacttt aaacatactg
    43321 aactcaactt ccttaaacga ctagataaaa gaatgctcag acagtttcgt ctgggctttt
    43381 ttgatgtgag aatgactgaa tgtcacttaa aatggaaaga aagagaaaat tttcatgatt
    43441 ttctaaaatt ttactgtaaa gacagttgac tattcataaa ataggtgtat aatattttgt
    43501 gaactactta acaaataaga ggtttcagct catgtcgact aaggttagaa atcttgtgta
    43561 taggcagttc attatctaat agagcgttgc gtccgaaagt ctatccagac acggctcttt
    43621 aaaaacaaaa ggagaaatga tgcatactta tttgcaaaag aaaattgaaa atatcaaaac
    43681 aaccctaggt gaaatgtcag gtggttaccg tcgtatggtt gcggctatgg ctgatttagg
    43741 attttcagga actatgaagg ctatctggga tgacctcttt gcccatcgta gttttgccca
    43801 gtggatttat ttgctggttt taggaagttt tcctctctgg ctggagttgg tttacgaaca
    43861 tcgtattgtt gactggattg ggatgatttg tagcttgaca gggattatct gtgtaatctt
    43921 tgtatcggaa ggtcgagcaa gtaattatct ttttggcttg attaactctg ttatttacct
    43981 tattttggcc ctacagaaag gcttttatgg tgaggtgctg acgacacttt acttcacagt
    44041 catgcagcca attggacttc tagtttggat ttatcaggca cagtttaaga aggaaaagca
    44101 ggagtttgtc gcgcgtaaac tggacggcaa gggctggaca aagtatcttt ccattagtgt
    44161 gctttggtgg ttggcctttg gcttcattta tcagtctatt ggtgccaatc gtccctatcg
    44221 tgattcaatc acagatgcaa ccaatggggt agggcaaatc ctcatgacag ctgtttaccg
    44281 tgaacagtgg atattctggg cggctaccaa tgttttttca atctatctct ggtggggaga
    44341 aagcctgcaa attcaaggga aatatctaat ttatctcatt aacagtctag ttggttggta
    44401 tcaatggagc aaggcagcta agcagaatac tgatttacct aactaggaaa agatgtttga
    44461 aagtgctgtt ttgagatttc gattaaaaca gatatagttg ataatcaagg atttatagta
    44521 tgaaaaagag gatcggcggg tcctcttttg ttgttgaaaa gataaaaaac tcagtaacct
    44581 agaaataaga caactgagct ttactctata ttcaattttt aggaatgaga aggtctagat
    44641 aaaattggac aacttcctgg tctgtgaaat cttgaccttt tttgagccac caggtcaatg
    44701 tctcgataaa gttggacacg accaagtgtt ggaggtaaga agtaggcata ttagggtggg
    44761 cttcttttaa attatcagct agcacggaat agacatggtg ttctagctct ttatggagtt
    44821 gacggaggaa gtagtcattt ttggaaaata gcagactggt gatatggtct tggtttttat
    44881 gaaaatggag aaagaggtgg gcgaggtagt cctcggttga aatggcttgc tctctttcaa
    44941 aaagatgatg gaagaggtag cggcagagct ggtccagaag aagctcctta ctttcatagt
    45001 gacagtaaaa ggtggatctt cccacatctg cgagatcaat gatatcctga acagtagtgg
    45061 cctcgtagcc cttagcattc aaaagttgta taaaagcttg atagatggct tttttggttt
    45121 tgctgatacg gcggtcaatg ttagtcatat ggacacttaa ggcaaattgt tcagaactga
    45181 ataaagctga cgttttgctt ctatcctttc tttgagtttt agtggataat gataatgaac
    45241 aaggtgttca taaacctatt ataacaaagg aatgagaaat atgaaggcaa aatatgctgt
    45301 ttgggtggct tttttcttaa atttgactta tgccattgtt gagtttattg caggtggagt
    45361 atttggttct agcgctgttc ttgctgactc tgtgcatgac ttgggagatg tgattgcaat
    45421 tggaatatca gcttttctag aaacaatctc caatcgtgaa gaagacaatc agtacacctt
    45481 gggctataag cggtttagcc tgctaggagc cttggtaaca gctgtgattc tcgtaacagg
    45541 ctctgttcta gtcattttgg aaaatgtcac gaagattttg catccgcaac cagtcaatga
    45601 tgaggggatt ctctggttag gaattattgc gattactatc aatctgttag cgagtctggt
    45661 ggttggtaag ggaaagacaa agaatgagtc tattctgagt ctgcattttc tggaagatac
    45721 gctagggtgg gtagctgtta tcctgatggc gattgttctt cgatttacgg actggtatat
    45781 cctagatcct cttttgtccc ttgtcatttc tttctttatt ctttcaaaag ccctttcacg
    45841 tttttggtct acactcaaga ttttcttgga tgctgtgcca gaaggtcttg atatcaagca
    45901 agtaaagagt ggcctggaac gattggacaa tgtggccagc cttaatcagc ttaatctctg
    45961 gactatggat gctttggaaa aaaatgccat tgtccatgtt tgtctaaaag aaatggaaca
    46021 tatggaaact tgtaaagagt ctattcgaat tttcctaaaa gattgtggtt ttcaaaatat
    46081 taccattgaa attgatgctg acctagaaac tcaccaaacc cataagcgaa aggtgtgtga
    46141 cttggaacgg agttatgagc atcaacatta gaaaaaagtg aaaaatactt gggtactatc
    46201 ttatttggaa tagagtaatt tctttattat ttaaatattt caaaaattgg taagagaaga
    46261 gcattgtata aactccagat atataattgt taatgataaa aatttttcga ttagatacaa
    46321 aatgcttgac ttggagtcaa ctcaaagtta tataataaga taagtgagtt agaatagcgt
    46381 gaattcagtg aatgaaatga gaggaggtta gcgtgtgaat attaaatctg ccagtgattt
    46441 gttgggaatt tcagcggata cgattcggta ttatgaacgg gttggtcttg tgccaccgat
    46501 tactcgtact gctactggga ttcgtgattt tcaagatcag gatatcgaag cgctggaatt
    46561 tattaagtgt tttcgttcgg cgggtgtctc tgtagatagt ttagttgact atatgtcgct
    46621 ctaccaaaag ggagatgaaa cgagagagga gaggcttggt attttagaag aggaaaagca
    46681 aaaattagag gagcgcttgt ctcagctaca gacagcttta aatcgtttaa atctcaaaat
    46741 taaactttat aaggaaggaa aattttaaat gaaatcagca gtatatacaa aggcaggtca
    46801 ggttggactt gctagcattg aacgtccgca gataatagaa gcggatgatg tgattattcg
    46861 tgtggttcgt gcgtgcgttt gtggttcaga tttatggagg taccgtaatc cagaaacgaa
    46921 agctggacac aaaaatagtg gacacgaagc gattgggatt gttgaagaag ctggggaagc
    46981 cattacgacg gtgaaaccag gtgattttgt gattgtccct tttacacatg gatgtggtga
    47041 gtgtgatgcc tgtcttgctg gatttgacgg ttcttgcgac aatcatattg gcaataattt
    47101 ggggggtgat tttcaggcag aatatattcg cttccactat gcaaactggg cgctggttaa
    47161 aatccctggt caaccttctg actatacaga agggatgctc aagtcccttt tgactcttgc
    47221 agatgtcatg ccgacaggct atcatgcggc gcgtgttgca aatgttcaaa aaggggacaa
    47281 ggttgttgtt atcggtgatg gggctgttgg tcaatgtgct gtcatcgcgg ctaagatgcg
    47341 tggagcatca caaattatcc ttatgagccg tcatgaagac cgtcaaaaga tggctatgga
    47401 gtcaggtgcg acagctgttg ttgcagaacg tggtcaagaa ggaattacca aggtgcgtga
    47461 aatcctcggt ggaggagcag atgcagcact tgaatgtgtt ggtacggagg ctgctataga
    47521 acaggcgcta ggtgttcttc ataatggagg gcgtatgggc tttgtaggag tcccacacta
    47581 taataatcgt gctcttggtt cgacatttat gcaaaatatc tctgtagcag gtggggcagc
    47641 ttctgctaca acatacgata agcaattttt actaaaagcc gtccttgatg gtgatatcaa
    47701 tccaggtcgc gtctttactt caagttataa actggaagat atcgaccaag cctataaaga
    47761 tatggatgaa cgtaagacaa ttaagtctat gattgtaatc gaataaaaaa cgaataggag
    47821 ttttagaact ctattcgttt tttatgttat cctattcttg atttagggta ctttctctta
    47881 atgtcagtct ggttcccagc atggtcaggc taggaatttt gcgaccgtgg aggacttcct
    47941 tgttaagaat atccatacct gctcgcccca tttcttcagt ataaactgtg atgctggaga
    48001 ggggaggata aacctgcttg gtcaggctgg tgtcgttaaa ggaaatgagg ctgacacggt
    48061 ctggtaggct gattccagct tcttggagcg cacgaagggc accgatggct aaactatcgc
    48121 tggctgcgaa aaatgctggt gggagttggt ctcccaagct ctgaatggct tccttcatca
    48181 agtcatagcc agactgggca gtaaagtttc cttgaaagac cagttcatca tgatagattc
    48241 cttttgcttg actgtagttt ctgaaatttt ccagccgctt atcctgaatg atttcttctt
    48301 ggtcagtcgt ttcttcaagg cctgttagaa tcccgatacg gtccattcct tggctgagga
    48361 aataatcgac aacctgtttc atagcagtgt aaaaatccgt gataatacag gtatgtccca
    48421 gggaaagtgt atcactgtct agaaagacca ggggtttttg gtattcttca aaggcagaaa
    48481 tctgagctcg gctaaatttt ccaatgcaga gaatcccaat cacttcctcg cttaaggtaa
    48541 aaggatggtc attaaaatag cgcaagatat catagtccaa ctcttgggct cttttttcta
    48601 ttcctaggcg aatctggtag tagtagaggt cgtccagctc cccttgttcg ctgacccatt
    48661 ggataatggc aatcttttgc ttgggtttat gggactcgcc tgtcttgagg tgcttggtgt
    48721 agcccagctc ttcagcaacg gttaaaatac ggtgtctggt ttcttctgta acagataggc
    48781 tctggtcgcg gttgaggaca cgggatacgg tcgcgataga gacagaggct agctgtgcaa
    48841 tgtcttttaa ggtagccata aatcctcctt gattaggtta gtatatcata tttttctgct
    48901 ttttactgat attttagtaa aattttagta aaaaggattg accttgggaa atcccttgga
    48961 tacaatagaa gaaaacgatt acacgttaag gtggcttaac ggacagtcaa aggagaaatc
    49021 atatgacaca acatcttact gctgaaactc ttcgcaaaga ctttcttgct gtttttggtc
    49081 aagaagcaga ccaaactttc ttttcaccag gtcgtatcaa tttgattggt gaacacacag
    49141 actacaacgg tgggcacgtt tttcctgctg ctatttcctt gggaacttac ggtgcagctc
    49201 gtaagcgtga cgaccaagtt ttgcgtttct actcagctaa ctttgaggac aagggcatta
    49261 tcgaagtgcc tctcgctgac ctcaagtttg aaaaagagca caactggacc aattatccaa
    49321 aaggtgtcct tcatttcttg caagaagctg ggcacgtgat tgacaaaggt tttgattttt
    49381 atgtttatgg gaatatacca aatggtgctg gtttgtcttc ttcagcatcc ttggagctct
    49441 tgacaggatt cgtggcagag catctctttg atttaaaatt agagcgtctc gatttggtta
    49501 aaatcggaaa acaaacagaa aacaacttta tcggagtcaa ctctggtatc atggaccagt
    49561 ttgctattgg tatgggggct gatcaacgtg ctatttacct tgataccaac accttagagt
    49621 atgatttggt gccacttgat ttgaaggaca atgtcgttgt tatcatgaac accaacaaac
    49681 gccgtgaatt ggcggactct aaatacaatg aacgtcgtgc tgagtgtgaa aaagcagtgg
    49741 aagagttgca agttgccttg gatattcaga ccttgggtga attggacgag tgggcctttg
    49801 accaatatag ctatctgatt aaagatgaaa atcgtttgaa acgtgctcgc catgctgtgc
    49861 ttgaaaacca acgtaccctt aaagctcaag cagcccttca agcaggtgat ttggaaacat
    49921 ttggtcgttt gatgaatgcg tcacacgttt ctctggagca tgattatgaa gtaactggtt
    49981 tggaattaga tacccttgtt cacacagctt gggcacaaga aggagttctc ggtgctcgta
    50041 tgacaggggc tggttttggt ggctgtgcca ttgccttggt tcaaaaagat actgttgagg
    50101 tctttaagga agctgtaggc aaacactacg aggaagtagt tggatacgct ccaagcttct
    50161 atatcgctga agttgcaggt ggcactcacg tccttgacta gtcaaaagga ggctctatgg
    50221 tgaccttagt agataaattt gtaacacatg tcatttctga aagttcattt gaggaaatgg
    50281 atcgaatcta cctgaccaat cgtgttttgg cacgagtggg agaaggtgtt ttggaagttg
    50341 agaccaatct ggataaattg attgacctca aggaccagct agttgaggaa gccgttcgat
    50401 tagagacgat tgaggatagt cagactgcgc gtgaaatcct tggtactgaa ctgatggact
    50461 tggtgactcc ttgtccgagt caggtcaatc gtgacttctg ggaaacctat gtccactatc
    50521 ctgagcaagc gatagaggat ttttatcaac tcagtcagaa aaatgactac atcaaactca
    50581 aggccattgc taaaaatatc gcttatcgtg ttccatctga ttatggagaa cttgaaatta
    50641 ccatcaatct ctctaagcct gaaaaggatc cgaaagagat tgtggcagcc aagttggtgc
    50701 aagctagtaa ttatcctcag tgtcaacttt gtttagagaa tgagggttac catggtcggg
    50761 tcaaccaccc agctcgcagc aaccaccgga ttatccgttt tgaaatggtt ggtcaggaat
    50821 gggggttcca gtattcgccc tatgcttact ttaatgagca ttgtatcttt ttagatggcc
    50881 agcatcgtcc catggccatt agtcgtcaga gttttgaacg tctgttggct atcgtagagc
    50941 agtttccagg atattttgct ggatctaatg ccgacctgcc gattgtgggg ggctctattc
    51001 taactcacga tcattatcag ggaggccgtc acgtatttcc tatggaattg gctcccttgc
    51061 aaaagacctt ccgatttgct ggttttgagc aggtcaaggc tggaattatc aagtggccaa
    51121 tgtctgtcct acgcttgact tcggattcca aagaggattt gattaactta gctgataaga
    51181 ttttccagga atggcgtcag tattcagatt ctagtgtgca gattttagca gagacagacg
    51241 ggacaccgca tcacaccatc acaccaatag cgcgtaagcg tgatggacag tttgagttgg
    51301 acttggtctt gcgagacaat cagacttcag cagagcatcc tgatggcatc tatcatcccc
    51361 acaaggatgt ccaacatatc aagaaggaaa atatcggctt gattgaggtc atgggcttgg
    51421 caatcttgcc accacgtctg aaagaagaag tagagcaagt cgctagctat cttgtaggag
    51481 aagctgttac agttgccgat tatcatcagg agtgggcaga ccaactcaaa tcccaacatc
    51541 cagacctaac ggataaagaa aaagcccttg caatcgtcaa ggactctgtg ggtgctatct
    51601 ttgtgcgtgt acttgaggat gcaggagtct acaagcagac agaacaaggg cagacagcct
    51661 ttatgcgctt tgtggaacag gtcggaattt tactagacta ggagctttct ccttgcacag
    51721 tcctataaaa agtagtatca tagtgtcgtt tgaaatatca ggaggaaacg atgaaagact
    51781 ttcattttga cgctatatct gcctttgaaa attacgaaat tgaaaaaatg agagatggtc
    51841 acgttgtggt gacgaccaaa gtagtgaact catcgctcaa ctactatggc aatgcccatg
    51901 gtggctatct attcacgcta tgtgaccaaa tcagtggttt ggtggttatc tcgctgggac
    51961 ttgatggggt gacactccaa tcctctatca actaccttaa ggcaggaaaa ctcgacgatg
    52021 tattgaccat taaaggagaa tgtgtccatc aaggtcgtac aacctgtgtg atggatgtgg
    52081 atatcaccaa tcaagagggc agaaatgtct gtaaagcaac ctttaccatg tttgtcacag
    52141 gccaaaggtc tgaggaaaga cgggtaagta tataaagagt aatttaatag agtgaggtga
    52201 attttggaat ttataagatt tgccttactc attttttgat attgatatga tttaattcta
    52261 aaatagaaaa tttagtaatg t
//
LOCUS       DAVLVZ010000005        51145 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000005 DAVLVZ010000000
VERSION     DAVLVZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51145
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.005"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(224..586)
                     /locus_tag="VYN77_000263"
     CDS             complement(224..586)
                     /locus_tag="VYN77_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1557786.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFETNIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
     gene            600..746
                     /locus_tag="VYN77_000264"
     CDS             600..746
                     /locus_tag="VYN77_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557787.1"
                     /translation="MKTKEQTRKLVSGCSKHCFEAVDRTDEVSNHTHGKATLTWFEEI
                     FEEY"
     gene            complement(877..1014)
                     /locus_tag="VYN77_000265"
                     /pseudo
     CDS             complement(877..1014)
                     /locus_tag="VYN77_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(1233..1922)
                     /gene="rplA"
                     /locus_tag="VYN77_000266"
     CDS             complement(1233..1922)
                     /gene="rplA"
                     /locus_tag="VYN77_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1557788.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2129..2554)
                     /gene="rplK"
                     /locus_tag="VYN77_000267"
     CDS             complement(2129..2554)
                     /gene="rplK"
                     /locus_tag="VYN77_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1557789.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFITKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2686..2823
                     /locus_tag="VYN77_000268"
     CDS             2686..2823
                     /locus_tag="VYN77_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557790.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(2863..3579)
                     /locus_tag="VYN77_000269"
     CDS             complement(2863..3579)
                     /locus_tag="VYN77_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1557791.1"
                     /translation="MKKRYLILTALLALSLAACSQEKSKNEDGEAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KVMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AESGLSLEEYYGFEGGDYVD"
     gene            complement(3590..4051)
                     /locus_tag="VYN77_000270"
     CDS             complement(3590..4051)
                     /locus_tag="VYN77_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1557792.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSLVQEAVAKTFTAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMKGHGLKGRGPVWWVPFEEMTSETCQAKPDEIKRLVKRLSSEVDKLLE"
     gene            complement(4053..5090)
                     /locus_tag="VYN77_000271"
     CDS             complement(4053..5090)
                     /locus_tag="VYN77_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1557793.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREIADYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRTVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSGLVD"
     gene            complement(5294..6619)
                     /gene="brnQ"
                     /locus_tag="VYN77_000272"
     CDS             complement(5294..6619)
                     /gene="brnQ"
                     /locus_tag="VYN77_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1557794.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6790..6882)
                     /locus_tag="VYN77_000273"
     CDS             complement(6790..6882)
                     /locus_tag="VYN77_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1557795.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(6925..7077)
                     /locus_tag="VYN77_000274"
     CDS             complement(6925..7077)
                     /locus_tag="VYN77_000274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1557796.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(7237..7815)
                     /locus_tag="VYN77_000275"
     CDS             complement(7237..7815)
                     /locus_tag="VYN77_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1557797.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKIARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMVKNLWFEAEVVPDLKKRIKTIL"
     gene            complement(7869..9269)
                     /gene="pepV"
                     /locus_tag="VYN77_000276"
     CDS             complement(7869..9269)
                     /gene="pepV"
                     /locus_tag="VYN77_000276"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1557798.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGA
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFDFAGPAKEYLDIAGKILLNDHEGESLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9282..9887)
                     /locus_tag="VYN77_000277"
     CDS             complement(9282..9887)
                     /locus_tag="VYN77_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1557799.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            10058..10858
                     /locus_tag="VYN77_000278"
     CDS             10058..10858
                     /locus_tag="VYN77_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1557800.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIVFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQPQLQ"
     gene            complement(10941..11093)
                     /locus_tag="VYN77_000279"
     CDS             complement(10941..11093)
                     /locus_tag="VYN77_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1557801.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLLLN
                     ENQRAN"
     gene            complement(11083..12927)
                     /gene="uvrC"
                     /locus_tag="VYN77_000280"
     CDS             complement(11083..12927)
                     /gene="uvrC"
                     /locus_tag="VYN77_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1557802.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(12967..13668)
                     /locus_tag="VYN77_000281"
     CDS             complement(12967..13668)
                     /locus_tag="VYN77_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1557803.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13747..14979)
                     /gene="murN"
                     /locus_tag="VYN77_000282"
     CDS             complement(13747..14979)
                     /gene="murN"
                     /locus_tag="VYN77_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1557804.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEYFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFCYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14984..16204)
                     /gene="murM"
                     /locus_tag="VYN77_000283"
     CDS             complement(14984..16204)
                     /gene="murM"
                     /locus_tag="VYN77_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1557805.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(16316..17095)
                     /locus_tag="VYN77_000284"
     CDS             complement(16316..17095)
                     /locus_tag="VYN77_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1557806.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17164..18825)
                     /locus_tag="VYN77_000285"
     CDS             complement(17164..18825)
                     /locus_tag="VYN77_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1557807.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19000..21222)
                     /gene="recJ"
                     /locus_tag="VYN77_000286"
     CDS             complement(19000..21222)
                     /gene="recJ"
                     /locus_tag="VYN77_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1557808.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLIHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21499..22257
                     /locus_tag="VYN77_000287"
     CDS             21499..22257
                     /locus_tag="VYN77_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1557809.1"
                     /translation="MVLVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDRMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            22297..23061
                     /locus_tag="VYN77_000288"
     CDS             22297..23061
                     /locus_tag="VYN77_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1557810.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTNKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23074..23751
                     /locus_tag="VYN77_000289"
     CDS             23074..23751
                     /locus_tag="VYN77_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1557811.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGATLLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23761..24420
                     /locus_tag="VYN77_000290"
     CDS             23761..24420
                     /locus_tag="VYN77_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1557812.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24678..25868
                     /locus_tag="VYN77_000291"
     CDS             24678..25868
                     /locus_tag="VYN77_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1557813.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(26189..27070)
                     /locus_tag="VYN77_000292"
     CDS             complement(26189..27070)
                     /locus_tag="VYN77_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1557814.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(27209..28537)
                     /gene="vncS"
                     /locus_tag="VYN77_000293"
     CDS             complement(27209..28537)
                     /gene="vncS"
                     /locus_tag="VYN77_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1557815.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKWVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(28534..29190)
                     /gene="vncR"
                     /locus_tag="VYN77_000294"
     CDS             complement(28534..29190)
                     /gene="vncR"
                     /locus_tag="VYN77_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1557816.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(29239..29322)
                     /gene="pep27"
                     /locus_tag="VYN77_000295"
     CDS             complement(29239..29322)
                     /gene="pep27"
                     /locus_tag="VYN77_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1557817.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(29502..30881)
                     /gene="vex3"
                     /locus_tag="VYN77_000296"
     CDS             complement(29502..30881)
                     /gene="vex3"
                     /locus_tag="VYN77_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1557818.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30933..31580)
                     /gene="vex2"
                     /locus_tag="VYN77_000297"
     CDS             complement(30933..31580)
                     /gene="vex2"
                     /locus_tag="VYN77_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1557819.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31593..32870)
                     /locus_tag="VYN77_000298"
     CDS             complement(31593..32870)
                     /locus_tag="VYN77_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1557820.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(33228..33398)
                     /locus_tag="VYN77_000299"
                     /pseudo
     CDS             complement(33228..33398)
                     /locus_tag="VYN77_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(33541..34140)
                     /locus_tag="VYN77_000300"
     CDS             complement(33541..34140)
                     /locus_tag="VYN77_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557821.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            complement(34277..35161)
                     /locus_tag="VYN77_000301"
     CDS             complement(34277..35161)
                     /locus_tag="VYN77_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1557822.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(35165..35551)
                     /locus_tag="VYN77_000302"
     CDS             complement(35165..35551)
                     /locus_tag="VYN77_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1557823.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(35544..36887)
                     /locus_tag="VYN77_000303"
     CDS             complement(35544..36887)
                     /locus_tag="VYN77_000303"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1557824.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(36969..37853)
                     /locus_tag="VYN77_000304"
     CDS             complement(36969..37853)
                     /locus_tag="VYN77_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1557825.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARVLFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHIEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDKYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(37865..38482)
                     /gene="cysE"
                     /locus_tag="VYN77_000305"
     CDS             complement(37865..38482)
                     /gene="cysE"
                     /locus_tag="VYN77_000305"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1557826.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(38498..40711)
                     /gene="pnp"
                     /locus_tag="VYN77_000306"
     CDS             complement(38498..40711)
                     /gene="pnp"
                     /locus_tag="VYN77_000306"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1557827.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(41270..42136)
                     /gene="metF"
                     /locus_tag="VYN77_000307"
     CDS             complement(41270..42136)
                     /gene="metF"
                     /locus_tag="VYN77_000307"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1557828.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(42200..44449)
                     /gene="metE"
                     /locus_tag="VYN77_000308"
     CDS             complement(42200..44449)
                     /gene="metE"
                     /locus_tag="VYN77_000308"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1557829.1"
                     /translation="MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLSAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(44710..44976)
                     /locus_tag="VYN77_000309"
     CDS             complement(44710..44976)
                     /locus_tag="VYN77_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1557830.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            complement(45060..45548)
                     /locus_tag="VYN77_000310"
                     /pseudo
     CDS             complement(45060..45548)
                     /locus_tag="VYN77_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            45704..46705
                     /locus_tag="VYN77_000311"
     CDS             45704..46705
                     /locus_tag="VYN77_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1557831.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSDILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            complement(46773..49175)
                     /locus_tag="VYN77_000312"
     CDS             complement(46773..49175)
                     /locus_tag="VYN77_000312"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1557832.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVGSPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIGTDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDTF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGHLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     SVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVIQKLRRQVRTIAEG
                     AGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(49252..49761)
                     /locus_tag="VYN77_000313"
     CDS             complement(49252..49761)
                     /locus_tag="VYN77_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1557833.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(49761..50807)
                     /gene="pheS"
                     /locus_tag="VYN77_000314"
     CDS             complement(49761..50807)
                     /gene="pheS"
                     /locus_tag="VYN77_000314"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1557834.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDVTVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
BASE COUNT        14496 a        11618 c         9609 g        15422 t
ORIGIN      
        1 ataaactaac ctcctcatta ttagcaaggc ttctactgat tataacctaa ttcttagact
       61 acttcaagac taactgtcca ataataatct acagataaat catccactct taatcaaatt
      121 ttattcaatc catctactca ttcccaaaag gttgctgtat aatgcaagac atttttctta
      181 ctgcttttac ttttccttac cctcaattta gtgagctaat aaattattct tcttcaaaag
      241 gttgagattt atctagtaac tctaaaacat taatggtagg tagcaacatt gcttcactgc
      301 tatcaattgc cgaaactatt gaaacaatag aacgtaaata aggataaaga atagcagtac
      361 catttacgta tagaatatcg gtgatatttt catctatatt tgtttcaaaa tatcctatta
      421 cagttacaga tatttttttc atggcatctt tgtcaactaa agaaacacta agttttacgg
      481 ctccatgact ataatcatct gtatgaccaa ccttaactat gggatataat atatcgaagg
      541 taaatgtgaa caaactgatt aggaaattta aaccaacttc taacaatgtg ttatactcaa
      601 tgaaaaccaa agagcaaact aggaaactag tctcaggttg ctcaaaacac tgttttgagg
      661 ctgtggatag aactgacgaa gtcagtaacc atacgcacgg caaggcgacg ttgacgtggt
      721 ttgaagagat tttcgaagag tattagaaag ctcgatggac tattccagat tcattccact
      781 agatttcgaa atcgagtagg ggataatctc tagcccctct cacaccaccg tacgtgccgt
      841 tcggcatacg gcggttcaac taactgttaa cgcatgtcat tcaaggtaat aatccaaaca
      901 cgaaaccagt ccacgttttt caaggactgg ttttgatata gcacgtttaa gtaccgactt
      961 ctgagatact aattgatagt ggtcgcccca gccagatacc ttatccgcta tataggcctt
     1021 attatcatgt tcctgttcgt agagccacga tttcgctatc ccttcctctg acgattctta
     1081 tttcctgcgt cagacttggg aatcgctatt gggttcaccg gtagcaggtg cccaccgagg
     1141 acttacacct cagatatacg acatgcccgt cgtacatcaa aaaaagttga gacatagcgt
     1201 cttcaacttt tttacattac tttaactact gattaaagtg agtttacgtc aactttgata
     1261 ccaacacctt gagtagttgt gatagtcaag tttgttacgt aagttccttt agctgtagct
     1321 ggttttgctt tttggattgt ttcgttgaaa gctttaaagt tttcaaccaa tttttcagct
     1381 tcaaatgata ctttaccgat gattgcttga acgttacctg cacggtcagc acggtaagtg
     1441 attttaccac ctttagactc ttcaactgct ttagcaacat ccattgttac agtaccagtt
     1501 ttagggtttg gcatcaagtt acgtggtcca aggacacgtc caagacgtcc aacaagagcc
     1561 atcatatcag gtgtagcgat aactacgtcg aagtccaacc aaccgtcgtt gattttagca
     1621 acaaggtcat cttcaccaac aaagtctgca ccagcagctt ttgcttcttc agcttttgca
     1681 ccacgtgcga aaacaagaac gcgtgaagtt ttaccagtac cgtttggcaa taccattgct
     1741 ccacggattt gttgatcagc ttttttaacg tcgatgttca agttgtaagc aacttctaca
     1801 gttgcgtcaa attttgcaaa gtttgtttct tttgcaagtg ctacagcttc ttctacgctg
     1861 tatgcttttg tgctgtcgat tttctcaaga gcagcacgaa gttgtttgct ttttttagcc
     1921 attttctatt ctccttgtaa gtggttcaat cgattttcat ctcccacgtc acttctcgaa
     1981 atgatgaagt cttgcgggtt ttcagtctat tagattcttt tcctacggcc ttaagctccc
     2041 ttgctgtacc caagtacaag catcagtcac ttgcctagta tgaaaagcaa ctaatagact
     2101 gctgtgagat tttctactgt gttattgatt agtcaacaac agtgaatccc atagaacgag
     2161 cagtaccttc gatcatacgc attgcagact ctacgtttgc tgcgttcaaa tctggcattt
     2221 tagtttctgc aatttcttgt acttgcgcac gagtaactgt agcaacttta gttttgttag
     2281 gtgtacctga tcctttttca acacctgcag cttttttcaa aagaacagca gctggtggtg
     2341 ttttagtgat gaaagtaaat gatttatctt cgtaaactga gataacaact ggaatgatca
     2401 taccagcttg gtcagctgta cgagcgttga actcttttgt gaatcccatg atgttgatac
     2461 cagcttgacc aagagcaggt ccaaccggtg gagctggtgt agctttacca gcagggattt
     2521 gcaattttac aagtttttcg acttttttag ccatttttaa atcctccttt gtggttttgg
     2581 cggtaattag agatttttac ctcccacaag tatgcttttc acatacccat ctattataca
     2641 ctatttatct ggaattgcaa gagaaaagtt tattttttat actcaatgaa aatcaaagag
     2701 caaactagga agctagccgc aggctgctca aagtacagct ttgaggttgc agataaaact
     2761 gacgaagtca gctcaaaaca ctgttttgag gttgtgaata gaaccgacgc agtcagtagg
     2821 taagacgacg ctgacgtggt ttgaagagat tttcgaagag tattaatcga cgtaatctcc
     2881 gccttcaaat ccatagtatt cttccaaact gagaccactc tcagcaattt ctttagcttc
     2941 ttttccgaca taacgaaggt gccattcttc tgccatatag cctgtttcct tttccttgcc
     3001 tttgagataa cggacaacaa aaccatagtc agccgcatgg tccaaaagcc attgggctgc
     3061 tttttcttct gtcaccaaat caccatcagt cccaatcaca tcaaaggcca agcctgtttg
     3121 gtgttcgcta tagccaggac gggcagagta acggtcagct gctgcctttc catcttgatt
     3181 gacataatct tggtagagtt tggtctgagt ttcataactt ctaaagccac tgtaatggtc
     3241 actgattggg aaacctgcct cttgcatcac tttgatgagt ttaaccaact cagccttggc
     3301 tgttggattt tcccctggat tatagtcttt agacaatgga tagtgtttgt tggctacgat
     3361 gatttcatcg tatttccctt gaatgctgta gtagtctcct ttattgacca cttctgcttt
     3421 cttctgggca gctccttgag acttactgcc gactgtccca tcagctttgg ctgtctgttc
     3481 tgtcttagct tctccgtctt cattttttga tttttcttgt gaacaagctg ctagactcaa
     3541 ggctagcaaa gctgtcaaga tgaggtatct ttttttcatt tctactcctt tattctaata
     3601 gtttatctac ttctgacgat aaacgtttga ctaatctttt aatctcatcc ggttttgctt
     3661 ggcaggtttc tgaggtcatt tcttcaaagg gaacccacca tactggacca cgtcctttga
     3721 gaccgtgacc cttcatatca cctcttcgtc taggaaacag atgccaatgg agatgggcat
     3781 cgccatttcc taaaagttca atattcattt tctcagcagt aaaggtcttg gcaactgcct
     3841 cttggactag actcatttct tcgagaaaac ggagtcttgt ttcctttttc aaatggtgca
     3901 attcggtaac gtgttcctta gctagaaaaa gactatatcc tgcaaaatac tggtggtctc
     3961 caatcacaag atagcctgtt tccaactctt tgacaaagta aggattttct tccttcttga
     4021 tgaggtcaat tctctggcaa atcaggcaca tattagtcta ccaacccact cttaagataa
     4081 gcatcaacca tacgctctgt tgcgaccacc gagtcaatat gagtacgctc ataagaatga
     4141 ctagactcga tacccgcacc aagaagagcg tgtttaactt ctgcccctgc agacatagcc
     4201 gctgaagcat ccgaaccata aaatggatag atgtccagct taaaaggaat atcttgctct
     4261 ttagccaagg caaccaagtg ttgacggaag tcatagtgat aaggacctga agcatccttg
     4321 acacagatag atactgtgta ctcatctgtc tgctggtcat ctcccatagc tcccatatcc
     4381 acagccagat attctactac ctgagcagga atgttagaat tagcaccatg tcctacttct
     4441 tcaaaaactg aaaaagcaaa atgagttgtt actggcaatt caatcttctc tttcttataa
     4501 atacgaagca ggttgagcaa aatcgctgcg ctgaccttat catccaaatg acgagacttg
     4561 ataaaacctg tatcagtcac aacagttcgt ggatcgaagc tgataaaatc accgacctca
     4621 atcccaagag cacgagtttc tttttcattg cttactttta catccaaacg cacttccata
     4681 ttgtcctgcg tgcgctctgc agttcctgca tccttgtaga catggcaaga agtttggtgg
     4741 atgaggatgg ttcctgatac cttttgacct gtgctagcca catgaacggt acagttttca
     4801 ccttcaatca tgttccaagg aaaaccaccg atacggtcca atttaagacg accatctggt
     4861 ttgacagtac gaacaatagc acccagcgta tccacatgag cagtcacata gcgatgttgc
     4921 tcatcatttt gacctttgat ggtcacattg acaccaccct tagctgtgcg aactggctgg
     4981 taaccaaaac cttctagagt cttgactaaa tagtccgcaa tctcacgagt aaagcctgtt
     5041 ggcgacgcaa tggctgtcag ttctttgata tattctactg tttgattcat ttcttcacct
     5101 cttttgctac ctattataac acatttatca tcggataaaa aaagaaaatc actcctgtag
     5161 acttgactac aaaagtgatt ttatactcaa tgaaaatcaa agagcaaact aggaaactag
     5221 ccgcaggctg tacttgagta cgacaaggcg acgttgacgc ggtttgaatt tgattttcga
     5281 agagtattag tgattattcc atttcaaaaa catcactttc ttgcttgttt ggtagaacca
     5341 atgagagcaa gattccaaca atggctggca ccaaccatgg gagagatgcc ttggcaaaag
     5401 gaagaacgct aacaagattt gcaagaaact caaccttaaa cgagcttcct agtacgcttg
     5461 caatggcaat aactgtaacc acagcaattg tcaactgcat acctggtttt gaaagggcaa
     5521 caaatttgtt gacaatgaca atcataacga tagcaatcgt gattgggtac aagataacca
     5581 gtactggaat tgagtacttg ataatcgcat caagacccaa attggcaata gcaaatccaa
     5641 tcaaggtaaa ggctgtcgca taaaccttgt agctgatttg tgggaagcgc tcattaaaga
     5701 actcagctgt tgacacaatc aaaccaacag tcgttgtgaa gcaagttacg gtaaccatag
     5761 ctgcaaggaa gagttgagct gttgagccaa agatttcttg agtggcttgt gacaagatgt
     5821 aaacacctgg tgttccaccc ttcatcgctt cagctggtac tgggaaatga tttccaagaa
     5881 aacctaaacc gatgtaaaga gcgctgaagg caagggcaac aacgatacca acaacccaaa
     5941 tagttgaaat gtattctttc ttacttgaaa atccaagttg tttcaaggtt tgaactgcga
     6001 ttacgctaaa ggccactgag gcaagggcgt ccaaggtatt gtaaccttct aggaaacctg
     6061 taccaaaggc agaagcttga taagcagctg aagcagcttg aggacttgtt ccaccatatt
     6121 tgatagctcc cagaacaacc aagataacaa tcaaaattgc aaagactggc gttaaaatac
     6181 gtccaatgcg gtctaagatt tttgatggat taagtgaaat caaataggct gccgcaaaat
     6241 acagaaccgt aaatacaatc aagccaagtc ctttatttgc atccgacaaa agggggctaa
     6301 tccctacttc gtaagctgtt gtagcagtac gtggggtagc aaagaatgga ccgattgaca
     6361 agtaaagaac tgagaggtaa agagtcgcaa accaaggcgc tatcttcgtt gaaatctcgt
     6421 agatatatcc tttaggattt agcgttccaa taataagggt caagacggcg ataccaacgc
     6481 ctgaaaagac aaaacctgcg atggcaggaa gaaaatgttc tccagataga gcacctagag
     6541 aaggcggaaa aatcaagttc cccgcaccaa aaaatattcc aaacaggagc aaacctgtta
     6601 gggtaccttt tttagccata aaaatctcct tcatatttat aaaatactga cctagtatac
     6661 cacgattagg agtctgaaaa aagcatcacg aggcaaatat taaatgtttt caattttctt
     6721 aaaaataaga attatctaaa aataaaactc tcctacccag tccacaccaa gtaggagaaa
     6781 tgtaaaattt taaagttctt caaaggctaa catgccaccc tggacattga taacattata
     6841 accttgttct aataggaatt ggcaagcacg cgccgatctc attccagatt tgcaaataat
     6901 ataatgcaag cgatctttgt ccaattaatc ataactatca gccaattgac taagcggtag
     6961 gttgtgggca ccttctaaat gaagagctgc aaactcatcc acttctctca catccactag
     7021 agaaagctgg tcgttttggt aaagctggta aaatgcgtca aaggctattt ctttcattct
     7081 ttttcttcta atactcttcg aaaatctctt caaaccacgt cagcgtcgcc tgaccgtgcg
     7141 tatgttaccg acttcgtcag ttttatctgc aacttcaaag ctgtactttg aacagcctac
     7201 gactagcttc ctagtttgct ctttgatttt cattgactat aaaatggttt taattctttt
     7261 tttcaaatct ggcactactt ctgcctcaaa ccaaagattt ttgaccatcc agatttgatt
     7321 tcgtggtgat gggtgaacta gcggaaaata ggctggcaga tagtctttat agtgtttcac
     7381 cctctccgtt accttcccac tgattttctc ctgtaaatag taggcttggg catattgccc
     7441 aatcaagagg gttaactgaa tatcaggcaa ttcctgtaag agctgcggat gccatttttc
     7501 tgcaaaacct gtacgaggcg gaagatcacc cgacttgcca tgtcctggaa agtagaaatc
     7561 cataggcaaa acagcaaaat aacctgaatt gtaaaaggta tcttcatcca cacctagcca
     7621 gtcccgcaag cggtcaccac ttttatcttt ccagtaaagg cctgcttctt gagttttaag
     7681 tcccggagcc tgaccgatga tattgatgcg agcaattttt ggcgctgcaa agagaggctc
     7741 aatgccacgc tctgtatagc tggcattctg cgaatccgcc atgatagcct gtttgattct
     7801 ttcgatttga gacatctact ttccctccaa aaagaagtcc aagcataaga tctcagactt
     7861 ctatcgtttt atttgatcaa ttcgtaaata gcttcggcat aaattgctgc tgctcggaag
     7921 agatcgtcca aggcaataaa ttcattggct tggtgcatgg tatcaattga gtctgggaac
     7981 atagcaccat aggcaactcc gcgctctaac aagcgaccaa aggttccacc accgatgact
     8041 tgttcatgac ctttaaggcc tgtttgtttt tcatagacat tcaacaaggt ttgcacaagt
     8101 ggatcttcca ttggcacata gtgaggcgtg tgaccgtgtt cagaaaggct aacagaaaca
     8161 actggcaagt tttcaaggat tgacttgatt tgttctggac ttgttccttt tggatagcgg
     8221 atgttgaggg caatggtatt atcagcgctt gtttcatcaa agcggaagac gcctgcattc
     8281 atagaaaggg cacccatctt ttcatccaca tgagcaatct tgagactttc accctcatgg
     8341 tcgttcaaga gaattttacc agcgatgtca aggtattctt tggctggacc agcaaagtca
     8401 aactggctaa ggaagagggc taggtaagtc gcaccattga cacctgaagc aggcatagca
     8461 ccgtgggctg atttaccaat gatcgtcacc ttgtatttgc cagcttcttc ttggagttct
     8521 cctctaagtt tgtgttctgc aacaaaggca tctagtttag cttgcaagtc agccaaatca
     8581 cctgaaacga ctgctgttgc tgattctggt accatatttt cacgtaaacc acctgtaaag
     8641 ctgtgaagac gggcagcacc tgtattttct cctgcaaagt ggaggtattc cgtgatattt
     8701 cctttttcac cattgatgat tggaaattca gcatctggag agaaaccaaa atctggtttg
     8761 gcaagtccta cgtgctcaaa gtagtagtcc atgtctgccc agcctgattc ttcgtctgtt
     8821 ccaacgatga agcgaacttt cttagaagtt ggaagaccca attctttgat gattttcaaa
     8881 ccatagtaac aagctgttgt aggaccctta tcgtccgaag ccccgcgcgc ataaaggcga
     8941 ccatctttga tagttggtgt gtaaggatct gtatcccaac cgctaccagc tggaaccacg
     9001 tccatatggg caaagattcc gagaacttct tctccatcac caaactcaaa atgtcctgca
     9061 tagttatcaa catttttagt tggataacca tcacggtctg cgatttcaag gaatttttcc
     9121 aaggctttta ctggaccagc tccaaatgga tgttgggcat cagccttgct atcatcacgt
     9181 tctgagttga tttccaaaag gctaaacaag tcagccaaga ggtcttcttt gcgtttttct
     9241 acttctgctg taaaatcaat tgctatcatt ttttcttctt cctatctttt ttcgatgatt
     9301 tcatctactg gcaagcggta gcttggttcc aatttgtcgt ctgtgtaacc cactgtgatc
     9361 aagagttctg ggcggaaacg gtcttcaatt tccaaaacct cattgacttt tgatttgtca
     9421 aatccaagaa taatgttaga accaattcct tggtctgtca atgcaagaac taagttcatg
     9481 gcaaccaaac ctgcattgag agctaggtag tcgctgactt gttgctcact gtaacgggca
     9541 aactcagctg gcagattttt cataaaatat tgaagttgct cttcagaaaa gttattagca
     9601 ccaccaacac gggcaatctt acgagcacgt ttggctaagt ccgtatctgt aaacaaggca
     9661 atggttacag gcgctgatga tacctgctca aagtttgaac cataagccaa ctttgccagt
     9721 tcagcatttt tctcacgaac aactacaaat ttccaaggct ggctgttgtg ggcgcttggc
     9781 gccaaggttg caatttcgat agccgtacgc acatcttttg gatcaacagg cttatcagtg
     9841 aaatgcttgg ttgcatgacg ttttttattt aattcaagaa atttcataat cgatttcctt
     9901 ttctaattct actgcttcca ttctaccata atttgaaaga gcttgcaggg atttttcatg
     9961 attaagtttt tctatcacag acataaaaga tatatattgg tttatcagga aagtttctga
    10021 taaactagca agtactttac atttgaatgg agacagtatg aaaaaattta gcctattact
    10081 agctatccta ccatttttgg ttgcctgtgg gaatcaagct acacccaaag agactagcgc
    10141 tcaaaagaca atcgtccttg ctacagctgg cgacgtgcca ccatttgact acgaagataa
    10201 aggcaatctg acaggcttcg atatcgaagt tctaaaggca gtagatgaaa aactcagcga
    10261 ttacgagatt caatttcaaa gaaccgcctg ggaaagtatc ttcccaggac ttgattctgg
    10321 tcactatcag gctgcggcca ataatttgag ttacacaaaa gagcgtgctg aaaaatacct
    10381 ttactcgctt ccgatttcca acaatcccct cgtccttgtc agcaacaaga aaaatccttt
    10441 gacttctctt gaccagatcg ctggtaaaac aacacaagag gataccggaa cttctaacgc
    10501 tcaattcatc aataactgga atcagaaaca cactgataat cccgctacaa ttgatttttc
    10561 tggtgaggat attggtaaac gaatcctaga ccttgctaac ggagagtttg atttcctagt
    10621 ttttgacaag gtatccgttc aaaagattat caaggaccgt ggtttagacc tctcagtcgt
    10681 tgatttacct tctgcagata gccccagcaa ttatatcgtt ttctcaagtg atcaaaaaga
    10741 gtttaaagag aaatttgata aagcgctcaa agaactctat caagacggaa cgcttgaaaa
    10801 actcagcaat acctatctag gtggttctta cctcccagat caacctcagt tacaataaca
    10861 ctcttcgaaa atctcttcaa accacgtcag ctttatctgc aacctcaaag ctgtgctttg
    10921 agcaacctac ggctagcttc ctagtttgct ctttgatttt cattgagtag aagaaaaata
    10981 tctcccagaa gccaatattc actggcccct tgatttttag catctgcaat cactgcttct
    11041 agcgccgtcg cattgccatg aacatctgat aaaattgcga ttttatggtt cattgtggtt
    11101 tccttccgtt tggtaatcta ctctttcttc tgctacttga ggcaaggctt ctccctgcgg
    11161 gttcaacttt ctttgcacag cctctgcaac gactctaggt accccaactt cgacaatctc
    11221 atccacactg gcttccttga ttttggtcaa agacttgaaa tgcttcataa gattttgctt
    11281 gcgtttaggt cccagaccat caatcccatc caattgagat gagaaagaat ttttggagcg
    11341 cagttggcgg tggaaggtaa tggcaaagcg gtgcacctca tcttggatgc gttggaggag
    11401 gaaaaattcc tgagaattgc gagacaagtc caccacctca agcggatctc caaagagcag
    11461 ttcatgggtt tggtgcttat cattcttttg cagcccagca attggaatat ccaagcccag
    11521 ttcctcttgg ataacctgct tagcgatatt gacttgacct tgccccccat caatcacaat
    11581 caaatctgga ggagtcaaag cctcacgctg tactcgacca tagcgtctgc gaatgacctc
    11641 tctcatgctg gcatagtcgt ctggtccaac aaccgttttt atcttgtact tacggtaatc
    11701 cttcttactc ggtttaccgt tgacaaagac caccatagcc gaaacagggc tagttcccat
    11761 gatattagag ttatcgaagg actcgatacg tactggggtc gggatttgga gcaaacgtcc
    11821 tagattttca atagctcctt gggtcttctc gacagatttc tctagcaggt taaatttctg
    11881 ctccagacta acacgtgcat tttttatggc tagattgacc agctgttttt tctctccacg
    11941 ttggggtttg agaatcttgg tatccaccaa agccttgaca gcttcttcgt caatatcctg
    12001 cggaatcagt acctcattgg gaactaggtg agatttttct tgatagaatt gtcctacata
    12061 ggtcaaaaaa tcctcatctg gatcattgaa gtaggggaag agattgacat cgcgctcgat
    12121 gagcttaccc tgacggacaa agaaaacctg cacacacatc cagcccttat ccacatagta
    12181 gccaaagaca tcgcgatttt ggagatcctt agccatgacc cgttgcttgg ttcgaagcgt
    12241 tccaatagcc tgaatcaggt cacggtattc cgccgcacgt tcaaactcca tagtctgggc
    12301 tgccgctgcc attttgccct ttagctcatc aatgattttg ttatcctgcc ctttcagaaa
    12361 atcagaaacc tcctgggcca tagacttgaa ataaatctca tccttcttac agatggtgtg
    12421 ggccatgcat tgaccgatat ggtaataaaa acagacctta gagggcgggt tggtacactt
    12481 acgaaaaggg aatatccgat ccagcaaccg cttgatttca ttggctgccc ccacatcggg
    12541 atagggtcca aaataaagac ctccgtcctt tttgacctga cgagtgataa tcaagcgtgg
    12601 atagcgctca ttggtgattt tgatgaaagg ataggacttg tcatccttga gcatgatatt
    12661 gtacttgggc ttgttttcct tgatcaggtt gatttctagg agaagtgcct caatattaga
    12721 ctccgtaaca ataaattcaa aatccacaat ttcagacacc agagcctctg tcttggtatc
    12781 atgacttcca cgaaaatagg accgtactcg attacgcaga tttttagcct ttcctacata
    12841 gataatggtg ccatttttat ccttatgaat gtagcaacca gggctggtcg gcaagagctc
    12901 tagttttgat ttaatcaagt tgttcatagt tccattatag caaaaaaagt agggaaagat
    12961 agtaccctac tcactagtct catatatttt tcggaggttt gcaacatcct cttcctggat
    13021 accataaaag gaacctgctg gttgcatgac agacttctca tctgtatggt ccaagccaat
    13081 cgcatgacct aactcatgtt ctgccgtatg gacaaggcgt tcataggagt agccatagta
    13141 tggattggac aaataataat gattcaaccg caccgttacg gacaagaatt gccctgttaa
    13201 gagattagtt tgactttccg cctctcctgc cacaggagtg cctccgtcgt tcatctccgt
    13261 agccgtaata tccgccttac tagactcagt cacgagttca aagttaaaag caccagtttg
    13321 attccagttc tggattgctt ctaaataagc ctcttgaaag cgtgaatcca tctgcggatc
    13381 caggtaaata cgagcagagg cctgtgccca ccttccttca gaatgctggt gactatctgt
    13441 ctggcttaac ttaggcagtt gccctgtttt ctcccattgg tcaatacttt gttgaccaat
    13501 ttttactgac cgttctgctt gctttagcgc tccttgaaaa tccccgttca gataccagag
    13561 aagtccgaaa gcaagcacac ataagagcac aactatccaa accagacgcc aaaacaaacg
    13621 ccacacaaaa gaaaagaaag cccctatcaa acgaaaaagc cagcgcatgt ctctccacct
    13681 ttctagcaaa taagaattat tttactaaaa gaagctaaaa gaaatctaaa tactgacttt
    13741 ttcatcttaa cgtcctacta tttttttgag taactggata gctttgtatt ttaaaggcga
    13801 tggatggtaa cagaaagtac ccgctttgcg tacaatatag ccattaaaat tctgtttaaa
    13861 acgcaaaaca ccatcacttc catcaaaaat cccttgaatc cctaggaagt tgtatttagg
    13921 tattccacgt tttatgcttt ccaacataac atatttttga agcagtgcag gggcatagaa
    13981 cttattaaac tcagtgtagg aaccactaaa gagataagtc gtttcctgag gcatataaac
    14041 aaataaactc ccagctaaaa caatatcttc ttctccatat ttttcaatca agtctcgcgc
    14101 ttctgctttt cgaacttcaa acgtttcaaa ttgactagaa tattctctca gttgattttg
    14161 ttttttctca gaatgaggat ttttactcaa atcaagtcgc aacttgtcca agttttcttc
    14221 tagtttactt tgttcacctt gcaatttgct catatagtcc gaaaaattta agcttgctat
    14281 gagaaactcc gcttgttctc caaaagtatc ataaaaatac tcataatatt ctaaactttt
    14341 atcactatat tctctacgtt cagaggtttc ttttgttata ttcttaaaaa tcgatagttc
    14401 ttcacgtttt aactttttca accgaatgcc aaaggtttca gcctttttca ccaagggttt
    14461 accctttttg ctaaaacttt taagcaaact cttttcagtt aattcagtta aatctttata
    14521 gtataaccaa tctggttctc cacctgggta acctgttgtt aagccatcaa attgataacc
    14581 taaatcagtc aaaccttgaa taatactttt tttctcagca tctattggat taccttggct
    14641 atcaaaagtt tgataagttt cataaggttt tacaagcaac tctaatacac cattttgctt
    14701 ggcatattct tttaactctg cataaaaaac tggaagagca tcttgttggg tataaatcgg
    14761 ccccgaattg agttccatat gcagaccacc cagcatgggc aagctataaa ccagagctgc
    14821 aacttgaatt tctccttctt gtttcaaagc aagataaaca attcgagccc ctcttttttc
    14881 tagcaaatcc cccatctgga cagattgcat aaaggaacga gaagaaacct gatcagaata
    14941 agtctgaaac tcttctttcg tgagtgttgt tagtgccata tacttacttt ctatgttttt
    15001 ttcttaatgt tttacggaaa tcaagagcaa gtcttaacag aggatagaga ggatgagtgg
    15061 gcattgtaaa ttcacccaag tattcttcaa tcgttggatt aaatttttcc ttaaaatgat
    15121 aaagtccacc attgagagag ttttcaacac cacctaaatt ttgccaaacc atacctcgct
    15181 caaaggcata gcgagccgtt tcataccatg ttaaaattgg tgcattgtaa cgtttaaaat
    15241 catcatccat accagcatat atattgacag aggtagtacc aaattccaaa ctcaaagtag
    15301 ccgctaaagg aactctcgct tgacctacat ctatatattc ctgcaagaag gtcaattcct
    15361 ctaacaaacg ttctttttcc ttcttctgcg cttctacttt tgaagttcga gtcgactcag
    15421 taaacgtctc ttccaaggct ctatttttcg ctaactgttc ttctaactct tgcgaacgtt
    15481 tagaaacatc caaggtggcc aaggtgatat aggccttgtc cttaaaatta tctaacaatt
    15541 ttttataata ggcttcattc ctcaaatgaa tctcttttcg cttctcagtt tttttcatca
    15601 actccgaaaa tgaatctaat agttccagtc caccatattg aatctcaagc cctttgtttc
    15661 gtgctgttcg aatagcctgt tttgttgact tggaaagttt atcttcttca aaattttcct
    15721 tgtatatttt cgcctgaata cgaggttgaa tggtgtctcc catttcctcc gtttttcctg
    15781 accaccttac tcccatttgt tgcaaactat caataatagc cagattttca ggaaattctg
    15841 tcttttcctg attgattaaa ctttgagata ggcaaatact tgggtcaaaa gtcacaaaaa
    15901 ccgctctctt actgcgagca taggacttaa tagactgaat ggcaaaattc aagagttctt
    15961 tatccccata atccaatata ggtcctcttg ggatgtaaaa cattttatag cctagcggaa
    16021 gagttctaat caaaatacta gctgtcgcca gtaatttttc ttccctgtaa acaccaaact
    16081 tctcatgttg ccaattagac ttaacttcct cccaagcact actttgtaat acattggcta
    16141 attcatgttc tttgacaaac tgatcatatt ctaatgtggg aatgccaatt tgataacggt
    16201 acatttccta ctctctttcc tccagtattt tattactatt ctactacttt cgtttgaaaa
    16261 gtctagtgaa aacatatttt attttagttc tactccccct atgctgaagt tcaaactaag
    16321 tcagtctctc ttctaatttg aaatcaattg gtagggttgt taaaaaaact tccaattgtt
    16381 tttcccagta gtaccactcg tgagttccag cgctatggct ataggtcaca tcaaaaccta
    16441 gttttttgag atttttcact gcgagattat tggcttcgta caagaaatcc tgttcgccac
    16501 accacgccca aagtttggtc tttttatccg attttttagc cagactttca agagaatagg
    16561 gactagttgt ccagtcttta atctctccaa aaacacctct ccagtaggct ggacttccca
    16621 gattttgact ttcaggagaa aagttttgaa agctgagggc acctgaaaaa ctagctgcat
    16681 gagaaaaacg atttgtcgca agagccagtt tgaagcagcc gtagcctccc atagaaagac
    16741 cagcgataaa ggtcttttca cgcttgctag tcatattagg gaagaagcgt ttcagaacct
    16801 gtggcaattc ctctgctaga gccgtgtagt agtcaaaacc atactgggta tcggtatacc
    16861 aaccattgct ggtattgggc ataacaacga tgagattagt tcctcgaagc aagcgttcta
    16921 cattggtccg cttaagccaa ctattatgat ttccagacat cccgtgcaaa aggtacaaga
    16981 cgggaatatc ttcacactct ggttcttcca ctcgattggc atcagggtag aggacattca
    17041 ccccccactc catatccaat acttgtgagt aatactcgat tttcattact gccatgattt
    17101 tctccttcta tttttctata agaaaaagcc gaaactcgac tttctctctg tttatttcaa
    17161 cgattatttt gcttccatga ctactggtaa aatagctgga cgacgcttgg tttggtcaaa
    17221 gagatacttg gtcaaattat ctcgaacctt ccctttaaga tctgcccagt caaagtcatc
    17281 tccttgaaga tactcttcta ccgtttggtt aatcaattct gaactttcac ggagaatatc
    17341 gcgactcttc ttgagataaa caaatccacg cgtgtgaaca cgagccctag ccacaatttt
    17401 cttctcacga cggttgactg taatagccac gatgaaaatt ccatcctctg acaagacctt
    17461 acggtcacga agaacaacat ttccaacatc accaatggca ttcccatcaa tcaagatatc
    17521 tcctgctgaa accgatccag ctggaacaaa gtctccattc tcgtaagcca tggtcgtccc
    17581 ctttttagga atgaagatgc gttctggcaa catcccaact gccatggcag ccttagcgtg
    17641 agcatccaac tcacgatact ccccttggac agggaagagg tacttaggtt gcaaaagatt
    17701 gatcatcagc tgcaaatcac gcacatttcc gtgccctgat acatgtaaac tttgggtaat
    17761 caatttgaca accccacctg cctgataaat catattttcc acacgcgcaa caaaggcttc
    17821 tttagcaata gacggagccg tagcaatata gaccaggtcc ccatccttga tttctacata
    17881 acgatggcga ccaatcgaca tcttacgaag tccattgata ggttcaccca tacgacctgt
    17941 ctcaagaata atcaactcat ggtcttcaaa gcgagacata tctttaggct taatcaagag
    18001 aatttcgttg gctaaagaca acttcttaag acgaatcgct gtgcggacga tattttcaat
    18061 atcaaatcct gtcaagacga tacgtcgacc tgttttatcc gcagcgtcaa aaatttgctg
    18121 aatacgagaa agattactgg aaacagctgc aacgatgata cgaccttccc agtcagcaat
    18181 agtttgggta atttcatccc taacttcact ttcactagcc acctgaatat tgctgtctgc
    18241 attggccgaa tcactgagga gagccaggac gccgtcacga ccaatctctg ccaaacgagc
    18301 aaaatcagtt gcataagatt cactagccgt ttggtcaaat ttgaagtcac ctgtataaac
    18361 gatgcttccc tccgatgtct tcaagacaat tcccagactc tctggaacgg agtaagtcgt
    18421 agggaagaag gaaaccactg tcccaccaaa atcaatctcc gtattctcat caatgacatg
    18481 gaaatcatta aatttcttaa cggcatcatt tcctttgaca aagagctttg ccaactcaat
    18541 ggtcaactca gacccaaata caggaacttt agcctctgcc aagagataag gtagagcacc
    18601 aatggcatcc gcatgcccgt gggtcaagaa aaccccagca atacggtcgc tattttcaaa
    18661 aaggtaatcc atgtttggaa tcaccacatc gacccctaat tgttcatttt caggatattt
    18721 taaccctaca ttcaaaacaa aaatggactc tccaatttca gcaatgtaca tattttttcc
    18781 attctcacgc acaccaccaa gtgttgttaa actgatatta ctcatttttc ctccaaaatc
    18841 ttagtttatc ttgattatag ggcaactcta ccctcttatt ctaaaagttc taatactttc
    18901 agccgaatct tttcctacca ttataccatt tttttgatga ttttcaaaat gaaggtctgt
    18961 tttcaaaaaa gagttgattt cccaactctt tcctaacttc tactcttttt ccatcaaaaa
    19021 atcataaatt tcttgcaccg tacccagcgc catcatttct tggtctttaa cggtttgttt
    19081 gagattttgg taaatttgac tttctcctat ctcccgcttt ggcgcctctt tattgactgt
    19141 catcacacca tcttttatcg tcacaaagcc tagttcttca aatacttgaa tcatcttgac
    19201 cagcaagatt tgttgaatat taagatatgc agccaaatct ttcagcttgt agcgaatatc
    19261 aaactctggg aactggtaaa tagtcttgta caatttggca aactgatctc tagtcccata
    19321 acctgtcaga taataggcct tgtcaatatc atttttgaaa tagacagcag agaaatgctg
    19381 ttcctgaaaa atggtcttca gctgagtaat atcctccgga atgtttttta cgacaacagc
    19441 ttcactagcc gcaagatttg gcagttctcc aggaaaatcc aagactggaa caccttctgg
    19501 caagactgca ttttttccac gaatgttaaa aagttgaaca ccttccactc gcgcatccac
    19561 catcatcaac tggagggcag tttggccatt ccattggttg acagacaatt taaccgctaa
    19621 ctctagattc ttggtttgag aaaactctgt cgcccatctg ccttgaccaa aggctaccac
    19681 ttcaaaactc gcctcaccct tggaaatttt cagctttaga tgggcattac ctgcccccat
    19741 agtacgagca ctttcgacct gaaaattctt gatataaaaa ataggtttct gattatccat
    19801 tccaaaagga gctaaacgtt caaaactttt gaccgtttcc aagctaagtg cctccaaatc
    19861 caactcttca tctaggttta acttattctt gccaccagca tctgcacctt tttcacgaac
    19921 ataatcttcc aaaacctgag ataaatctga gagttgctca acttccagcg tcatacccgc
    19981 tgcacctgca tgacctccaa aggcgatgaa gaggtctcga tagggatcca gagcttcaaa
    20041 aatatcgacc gcttccacac tacgagcact gcccttggca cgaccgtctt ctatattaag
    20101 aacaatgact gtctgtccca attcttccaa taaacgacca gccacgattc ctagaacccc
    20161 aggattccag ccttccttgg ccaagacctg aaccttcttc tcaggatcca ccatggtctt
    20221 ggcttcttca tagatagact gaacgatttc cttgcgctct tcgtttttct ggtgaatcat
    20281 aagggcaatc tcatgcgctt cctcatcatc aaatccagtc aacaaatcaa tggcaggatt
    20341 gggatcatcc aagcggccca aggcattcaa acgaggagca atctggaaac caaccgtttc
    20401 ttctgttact tcattagcag caatcccagc catgtccagc atttcttgca gaccaatgcg
    20461 ctgggtatga cccaacattt ccagaccata ttgaactaag atacgatttt catccgtcag
    20521 actcaccata tctgcaatag ttccaatagc gaccaaatca agcaattcca cttgcacttc
    20581 ttctaacagg gcacaagcca acttgaaagc aactccacaa ccagccaaat atttaaaagg
    20641 ataatccgca tctggatgtt caggatggac aatagcataa gcatctggca gggtttcagg
    20701 catggaatga tggtctgtca caatgacatc tactcccata gactgagcca atgcaatagc
    20761 ctcatgacca gcaaccccat tgtccaccgt cacaatcaag gaaattcctt cttgctcgat
    20821 aaagtattta taaacactag cattagggcc atagccatcg gtaaaacgat ttggcaggta
    20881 aactcggcac tcagcaccaa gttgttccaa actttccttc acaatagaag ccgaagtcat
    20941 gccatccgca tcatagtctc cataaacgag aatattttcc ccttcttcaa tagcctgacg
    21001 aatccgctcc actgccttgt ccatatcatg gatcagataa gcatcatgta agtcctccaa
    21061 ggaaggttct aaaaacttct tcagactttc ttggtcctga atccctctct caaacaataa
    21121 ccgagccacc tcaggaccca gtccagcctt cttggctatc tttgtaaaat ccgcatcttc
    21181 tacctgcagg gcaaactgcc attcataagt aggtgttatc aaaaagacat ccactctttc
    21241 taagaattct attgcttcat tataacatga tttagacgtt ttctctatcc agattgcttt
    21301 tttataacat tttatagtat attgaaacta gaatagtaca cctctacttc taaaatattg
    21361 ttagaaatcg atttgactgt cccgatcgat ttttcctgtt cttatttcac tatagtgaat
    21421 tttttcagat atgatttgac aagaacattc ttttattgca gaatcgtgtt ataaaatcta
    21481 acataaagga gttctctcat ggttttagta gaatttaaaa acgtcgaaaa atattacgga
    21541 gactaccacg cactccgcaa catcaatctc cgttttgaaa aaggacaagt tgttgtcctg
    21601 cttggacctt ctggctctgg gaagtccact cttatccgta cgatcaatgg tttagaggct
    21661 gttgacaaag gaagtctcct agtcaatggg caccaagttg ctggtgccag ccagaaagat
    21721 ttggtacctc ttcgcaagga agtcggcatg gtttttcaac attttaacct ttatccacac
    21781 aaaacagtgt tagaaaacgt gacacttgcg cccattaaag ttctaggaat tgataaaaaa
    21841 gaagctgaaa aaaccgccaa aaaatatctg gaatttgtaa atatgtggga caagaaagat
    21901 tcctatcccg ccatgctatc tggtggacaa aaacagcgga tcgccatcgc tcgtggtctt
    21961 gctatgcatc cggaactcct cctctttgat gaaccaacat ctgctcttga tcctgagact
    22021 atcggagatg ttctagcagt tatgcagaaa ctggcgcgtg ataggatgaa catgatcatc
    22081 gttacccacg aaatgggctt tgctcgagag gttgcggacc gcattatctt tatggccgac
    22141 ggagaagttt tagtagatac gacagatgtc gataactttt ttgacaatcc aagcgaacct
    22201 cgtgcccaac aattcctcag caaaattatc aaccacgaaa gtgacaaagt caaataagga
    22261 ggcgcctatg aaaaaaaatt ctttttatca gcattattga ttagcctttt cggccttgct
    22321 gctgccaaac cagtccaggc tgatacaagt atcgcagaca ttcaaaaaag aggcgaactg
    22381 gttgtcggtg tcaaacaaga cgttcccaat tttggttaca aagatcccaa gaccggtact
    22441 tattctggta tcgaaaccga cttggccaag atggtagctg atgaactcaa ggtcaagatt
    22501 cgctatgtgc cggttacagc acaaacccgc ggcccccttc tagacaatga acaggtcgat
    22561 atggatatcg cgacctttac catcacggac gaacgcaaaa aactctacaa ctttaccagt
    22621 ccctactaca cagacgcttc tggatttttg gtcaataaat ctgccaaaat caaaaagatt
    22681 gaggacctaa acggcaaaac catcggagtc gcccaaggtt ctatcaccca acgcctaatt
    22741 actgaactgg gtaaaaagaa aggtctgaag tttaaattcg tcgaacttgg ttcctaccca
    22801 gaattgatta cttccctgca cgctcatcgt atcgatgcct tttccgttga ccgctctatt
    22861 ctatctggct acactaataa acggacagca ctactagatg atagtttcaa gccatctgac
    22921 tacggtattg ttaccaagaa atcaaataca gagctcaacg actatcttga taacttggtt
    22981 actaaatgga gcaaggatgg tagtttgcag aaactttatg accgttacaa gctcaaacca
    23041 tctagtcata ccgcagatta aggaggatac cctatgacag atttatcatc ttggacagcc
    23101 tattttcagg attttggaca atttttcaat ggtttcctct ttactcttgc cctagcgatt
    23161 ggatccttta tcctcgccat ggtcttagga atcttctttg gtgccatgtc aaccagtaaa
    23221 cgtccaattt tacgcatttt agctcgcatc tttgtggaat tttaccaaaa tactcctctc
    23281 ttggtgcagt ttgttattgt tttttatggc ctacctctta tcagtgacca tatcatcatg
    23341 attccaattt attggacagc cgttctctgc gtgggactct atcacggcgc ttatatcgct
    23401 gaggttattc gttcagggat tcagtctatt cctagtggtc agatggaggc cgccttgtcg
    23461 caaggtttta cctatatcag tgccatgcgc ttgattacct tgcctcaggc cttccgcatc
    23521 attctccctc cattgaccaa ccaaattgtt aacctcatca agaacacctc tactgtagct
    23581 atcatctctg gagtagactt gatgtttgtg actaagtctt ggtcggctct caacggaaac
    23641 tatatcccag cctttttagg tgctactctt ctctactttg ccctatgctt ccctgttgcc
    23701 cagtttggtc gcaagatgga gcaagccaac aaaaaagcct attcacttta ggaggttact
    23761 atggaatcta ttttagaagt tttaacccca gataacctag tctttatctt taaaggattt
    23821 ggcttgaccc tctatatttc tctgattgcc atcatcctct ctactatcat cggtacggtg
    23881 ctagctgtca tgagaaatgg caaaaatcct gtcttgcgaa tcatctccag catttatatc
    23941 gaatttgtgc gcaacgttcc caaccttctc tggattttta ctatcttttt ggtgttcaaa
    24001 atgaaatcca caccagcagg tattacagcc tttactctct ttacatcagc agccttggct
    24061 gagattattc gaggcggtct caatgccgta gacaagggac aatacgaagc aggaatgtca
    24121 caaggcttca cctcagccca aatcctctac tacatcattc tcccacaagc catccgcaaa
    24181 atgctaccag ccatcatttc tcagtttgtt accgtgatta aggataccag cctcctctac
    24241 tctgttatcg ccctacaaga actctttgga gccagccaaa ttctcatggg ccgttatttc
    24301 gaaccagagc aggtcttcag tctttgcatc ctgattgccc tcatctactt cagctttaac
    24361 ctagcaattt ctagcctgtc tcatatgcta gccaaacgtt ggcaacaagc tgcagaataa
    24421 tactcttcga aaatctcttc aaaccacgtt agctctatct gcaacctcaa aacagtgttt
    24481 tgagcaactt gcggctagct tcctagtttg ctctttgatt ttcattgagt ataaacagcc
    24541 actctccagt tcaattggag agttttttga tgagagtaga tatttataga cctcaatttg
    24601 acaccataca ttttctatga taaaatgtaa caaatttatt tacaacaaat cgaaatctaa
    24661 atgaaatgga attttctatg aaatcagtca aaggactact ctttatcata gctagtttta
    24721 tcttgactct tttgacttgg atgaacactt ctccccaatt catgattcca ggactagctt
    24781 taacaagcct atctctgact tttatcctag ccactcgtct cccactacta gaaagctggt
    24841 ttcacagttt ggagaaggtc tacaccgtcc acaaattcac agcctttctc tcaatcatcc
    24901 tactaatctt tcataacttt agtatgggcg gtttgtgggg ctctcgctta gctgctcaat
    24961 ttggcaatct tgccatctat atctttgcca gcatcatcct tgtcgcctat ttaggcaaat
    25021 acatccaata cgaagcttgg cgatggattc accgcctggt ttacctagcc tatattttag
    25081 gactctttca catctacatg ataatgggca atcgtctcct tacatttaat cttctaagtt
    25141 ttcttgttgg tagctatgcc cttttaggct tactagctgg tttttatatc atttttctat
    25201 atcaaaagat ttccttcccc tatctaggga aaattaccca tctcaaacgc ttaaatcacg
    25261 atactagaga aattcaaatc catcttagca gacctttcaa ctatcaatca ggacaatttg
    25321 cctttctaaa gattttccaa gaaggctttg aaagtgctcc gcatcccttt tctatctcag
    25381 gaggtcatgg tcaaactctt tactttactg ttaaaaattc aggcgaccat accaagaata
    25441 tctatgataa tcttcaagcc ggcagcaaag taaccctaga cagagcttac ggacacatga
    25501 tcatagaaga aggacgagaa aatcaggttt ggattgctgg aggtattggg atcaccccct
    25561 tcatctctta catccgtgaa catcctattt tagataaaca ggttcacttc tactatagct
    25621 tccgtggaga tgaaaatgca gtctacctag atttactccg taactatgct cagaaaaatc
    25681 ctaattttga actccatcta atcgacagta caaaagacgg ctatcttaat tttgaacaaa
    25741 aagaagtgcc cgaacatgca accgtctata tgtgtggtcc tatttctatg atgaaggcac
    25801 ttgccaaaca gattaagaaa caaaatccaa aaacagagct tatttacgaa ggattcaagt
    25861 tcaaataagt aatcatactc tcctgggatt tcccaggaga gttttttata gccaaatgaa
    25921 ataagacagg aacgatggaa ttttggcatt caaatcaatt gataagaatg ttttatagtg
    25981 gagtgaaata ggatacgaac aaattgatta ggaaaatcaa atgaatttat agaaatcttt
    26041 tagcagttat gttgtcctat tctagtttca aaacgctata gaagcagcat tgtgctagtc
    26101 tagattcagt ttactatact aaaacgagta gcttgaaatc aaaaaaccca ccctcacagg
    26161 caggttttat ctgtattatt cagctagatt atgctttacc ttctgaaccg aatacgtcga
    26221 tacgttcttc aaccgatgct tggatagctt ttacaccgtc agccaagaat ttacgtgggt
    26281 cgaagagttt tttcttgtcg tattctgctt cgtttgcttc gtagtcacga gcaaatttac
    26341 gagttgcgtt agcgaatgcg atttggcatt ctgtgttaac gttaactttg gcaacaccaa
    26401 gtttgatagc tgcttggatt tgctcatcag gaatacctga tccaccgtgc aatacgattg
    26461 ggaatcctgg aagagcttct gtcaattttt gcaagtggtc aaggtctaga ccttcccagt
    26521 ttactgggta agggccgtgg atgttaccga taccagctgc caagaagtcg ataccagttt
    26581 caaccattgc tttagcgtct tcgattggag ccaattcacc tttaccgatg attccgtctt
    26641 cttcaccacc gatagtacca acttcagctt ctactgagat acctttagcg tgtgcttttt
    26701 caacaacttc tttagccaat ttaaggtttt cttcaactgg aaggtgtgaa ccgtcaaaca
    26761 tgattgaagt ataaccaact tcgatacact caagtgcatc ttcgtagtga ccgtggtcaa
    26821 ggtggatagc tactggtaca gtgataccca ttgattcaac aaggttagcg atcaagttgc
    26881 gagcaacttt gtaaccaccc atgtatttag cagcacccat tgaagtttgg atcaaaactg
    26941 gagctttttt agcttctgct gcgcgcaaga tagcttgagt ccactcaagg ttgtttgtgt
    27001 taaatccacc aactgcataa ccgttgtcac gggctgcttg gacaaatttt tctgctgaaa
    27061 cgattgccat tttatcaggc ctcctgtata tttttatggg tcatcccatt tacattgttc
    27121 attttatcac tttttgccaa aaaaatctag tttttcccgc agtttcgatt gattttcttc
    27181 taactccatc tatgtaaacc ctttctccct agtcttggac gacttttgga aaatctataa
    27241 agaaggttaa acgattctcc tccatctcga aacgataagc taatttttca tgttctaata
    27301 gactcttaac cacaaagagc cccataccag accccttgac cttgcgacta gcattgtcag
    27361 aaaaagactg ggctagtttt tcttgttcct ctgagctaca gctattttcg ataaaaagtt
    27421 ctccttctct ttctccaatt cgaactaagc cacctggaac agagtgctta atggcattgc
    27481 tgatgagatt agaaagaatc aacttcataa ctgatgggtt tagataagcc tgctgatggg
    27541 tcaaactatt gtctatctgg agctctcttt cctttgctag caaggcataa tctttgacca
    27601 gattttgcgt catctggagg aggtcaattg tttccctatc atctcgcaat tcctgcacag
    27661 aagagaggga aagtatctgc agaacatggt gattgagttc atccacaatc cccaaggcaa
    27721 ctcccagata ctggtctcta tccttataac gaccgatatt ctctctcata ttttcgatta
    27781 ggattttcaa actagccagc ggtgttttca attcatgaga agctcctcgt aggaattcga
    27841 ccttcatctt ctccagctgg agaatggctt cattcttttc atgcaagtcc gcaataacag
    27901 tcaagagatg ctggtagagg ctattgattt gttccttgag attacctatc tcatccttag
    27961 aatccacgcg caatcgcact tgggaatcca ggtccatcat ccgacgggtc acccacttga
    28021 tttccaaaat cggtgcaaca atagtccgag cgtagatgta ggccaccaag agggaaatca
    28081 gaaaggaggc cagcaaggta tagggaagaa actggagact gatttgctcc gcttcctttt
    28141 gtaaatccat ggaagctaga aactggagaa tcatagtacc accgtcttgc gttttcacct
    28201 cgcgctcctc aataaagaga gaggttgtct ggcggtctgt gtccagagga agactgtcct
    28261 tgacttctaa cttgtcctcg gtcatctcac ctttgacggt ccccttgata tcactagtct
    28321 gggaatacaa gtctaacact tgctcgatac tctgcctatc tttcccttct agggactggg
    28381 caatggctgt tgccttttga ccaatggttt cctgacgatg actcagataa gtcgaaggaa
    28441 aaagaaaata aatagctaaa tgaaggcaga taaccagaac actaaatatc gagaaggtat
    28501 agataaatat ctttgcaaat aaacctgttc gtttcatttt cgctccaatt tataaccaac
    28561 attgcgcaca gtgaggatac aatccaagtc tagctttttc cgcaattcct tgatataaac
    28621 atcaataaca cggtcaaagg gaacctcatc tgtcgctttc cagacagcat cgataatctg
    28681 agaccgagtc aaggcccggc cttcattttt cactagatag tccagaattt ccaactcttt
    28741 ggcattgata ggcacttctt gacctgcgag gcttgcactg tagctttcaa agtccacctt
    28801 ggtatccttg taagaaaaga ttcgtcctgt atcgtagtag cgcttgaaaa tcgcgtccac
    28861 cctcactttt aagagggaga gagagaaagg tttttccaga tagccatctg ccaaagaggc
    28921 aaaggcactc atcttgtatt cctcatcctg aaaagctgtc aacatcaaga caggaacctg
    28981 actggtttta cgaatctcag ctaggacttc taagccgtta agcttgggca tctggatatc
    29041 cagtaaaacc agggccacct catagctaga aaattgctcc agagcttcct gaccgtccgc
    29101 tgcctcaata gtttcatagc cacaatccgt caaataatca ctgaccccct cacggatcat
    29161 ctcttcatct tctacaatta aaattttcat actttaactg ctctctattt tttatttttc
    29221 ttagaataaa tacctactct attttctatt atagtctctt gctggccttt tgtctgtaag
    29281 caactgatca ctagataaaa cgttgtgaaa ttcctttctc ataaattcca taactttagt
    29341 atattatatt taagcactaa agtacaaaga aagcaactga aagcaatgat tttcaccact
    29401 gctttcagat ttattttgaa ttgttaaata gctattccta tccactattc ttgaatagaa
    29461 acacaagatg caatctttat tccagactca ttttttcaaa tttattcacc atccagcaag
    29521 agctcttttg gttgttttct aaggagattg cttgaagcaa gcgccataac gagaaccact
    29581 agaaccaagg caaggacaaa aatgatgata aagtctgatg tctgaatgga aatgtctagg
    29641 ctcgacaagg tcttgctaaa gccatctact tctgcaccgc caccaaggtt agaggcttga
    29701 gccgccttac tagcctgttt ggcaacacct gaagtcacat tggcaaggac agtgtttcca
    29761 attgcacggg cagtgtaatt agctaggaag taagcagaaa ctagagcagg gatagcaatc
    29821 aagatagatt cggtgatgaa ttgacccaag atacttgcct gcttgaggcc gatagagagg
    29881 agaattccca cttccttgcg acgggcgttg atccaaaggc tgagcaagag ggcaaggagg
    29941 agaactgaga agctcaagct accccagaag aggaggttgg ccatcttgta cataccagag
    30001 atagattgct caagagctgg gtagttagag gagctcttga cgagtgtgta gctcttccag
    30061 ttgataccac tgatgccatt caactctttc ataacatcat ccaagttctt gtctgctgtt
    30121 acaaagaagg ttgcgtcccc ataaatggct gtgtcttctg tgtatccata aagttttgca
    30181 gcagtgtgaa tgtctgtaat agctgtgttt tcgtaaagtt cttgtgagta ggttactgct
    30241 gacttattat gaccatcaaa gagtcccttg attgtcactt caactgtttc cttggctcct
    30301 ttttcattat ctgcatcgta gatattagag tccagtttaa ccttgtcccc tactttccag
    30361 ccgtgtttgg ctgccaaatc cttgtgcaag aggattttat ccttgtcgtc gttggttaag
    30421 tgctctcctt cgactagttt ataagaacca gagacaaact tgtcttcttt agaggagtca
    30481 ttgacacctg taatcatcaa gctacttcca aaacgtttgg cacgatcagc agtgagattc
    30541 ttcttggttt ctggcgtttc aatcaggtca tatccagtca aatctccgat agcgttgata
    30601 cgtttgacat aagactcaat ggccttgttt tcggtgattt ttttgatatc ttcacccttg
    30661 atattcccag caccacgagg cgttccttgg ttgacgcgac gattgatttg catggagaag
    30721 ctattggtga tatttttaaa ggtctcctga gaagccttgg cagtagctcc cttgattgac
    30781 aagccgacca aactcaagct cgccatgagg agaataatca ggaagatgac aatcgatttg
    30841 aaaaacttcc ttgtaacata ggcaaatgcg ttgtgtaaca tagattccct ttctagattt
    30901 tgttttaatc attctatcaa aataagctca aattatttac tagtattgcg cgtttcagtc
    30961 agtttcttat cctttaattc aagtgtaata tctgacgctt gtgccacttc tttactgtga
    31021 gttacgacaa tcacacattt acctgttttc tgggcaagtg atttgagtag ttcgacaata
    31081 tctccagcag ttttaggatc cagatttcct gttggctcat cagctagaat aactggagct
    31141 tctgagacca aactgcgagc aatggcaaca cgttgctgtt gaccacctga taactggaga
    31201 acattccgct tgatctggct ttcatccaaa ccaagctcaa gaagtgtatt cttgcttgcc
    31261 tttttgttga ccaatcggat attttccagc ggagaaagat aatctatcaa gttataattt
    31321 tgaaagacca gggaaatatg gtgcatgcga tggtaagagt agcccttctt acgaatatcc
    31381 tctccttgaa aaaggataga accttcaaca ggactatcta gaccagcaag tagggacaag
    31441 agtgtggatt ttcctgctcc tgactcccca ataatactgt aaaattttcc aggttcaaaa
    31501 ttataatcga tctgatatag gactgcttca gcagtattct tataacggta ggtaacatct
    31561 tgtaattgta ataaagtcat gatttctcct tcttaactaa tagatgataa aatttctttc
    31621 ggtgattttc taaataagaa taggaaacaa agggctacag ataagcaact aagcagaact
    31681 agaaaaacat aggattctgc aaaagataag atgctagttg ataaactgct tgctttggct
    31741 agtgtatctt gtaagcttgc ctgatctcca cttgctagta gagtttggag taggtaagtt
    31801 gtgattgcgt ttcctgcaac aaatgctgga agcaaagctc caagagatac caaaactacc
    31861 tctaaacaga attgtaggaa gatcgagctc ttgccttttc caagtgcaag taaaatcccc
    31921 acttcataga cccgttctct caaccagaga gacaaaacca gaattaaggc tccagctcct
    31981 gctatcaaca tcccataaag gaagatggtc aggaaggttt ggaaagttgc aactgagtct
    32041 ttgatttgtt caaaagcctt gttttccttt tcgacttggt agccttgatt ttccaaggcc
    32101 aagttttcta cctgcttcat gagtccgtcc atttccttag gattttctac atagaagcgt
    32161 gctgcactga cttgagcttc actattgccc aaaagggttt ggctactttc atagtctgta
    32221 aagacttgat tttcactgaa gtcagaagac aagcctgtga atttctcttg ttttttacca
    32281 gaaaagatgc cgataatctc aaactctact gtttgtcctt ttccagattc agactgacca
    32341 gcatccaagc caatcttgtc atgaagcgaa agaccgttct tcttagccaa ttcttcgtgg
    32401 ataaggattt tcttggaatc ccctttttga aggtgtcgcc cttcttttag attgaaagcc
    32461 gaactggtaa aggttacatc cttggatgaa tcctcaagag ccgttaagct aaccaagtta
    32521 ttgtctgcag ctgataaatc atcacgctcc acgctctgct cgccagtcac tgcttccttg
    32581 tcttttagtt ttgcgaccgt ctcaagttca ggagagacat tttccagccc cttaatcttg
    32641 cttacagatg ctaggtctga caacttgaat gtctgaccat tctctatctt cttaatagaa
    32701 aaagatgtat tgagtgattt ataaagattg ctttctactg ttttgttgga cttcatcaga
    32761 gtcaaacagg ctgaaattcc ggccaataag accaataaaa tcagaaataa aataaaactt
    32821 ctcagtcgct ttctgctgac ataagcccaa gatctttgga ttggattcat ttgtcacctc
    32881 catatttgta agactattat aaaacccaaa tatgaaatgt ttatgaaata cgaaaaaaaa
    32941 aatatcgagt aggggataat ctctagcccc tctcacacca ccatacgtgc cgttcggcat
    33001 acgacggttc aactaacttt taacgcatgt cgttcaaggt aataatccaa acacgaaacc
    33061 agtccacgtt tttcaaggac tggttttgat atagcacgtt taagtactga cttctgagct
    33121 actatagtag attgaaacta gaatagtaca cctctacttc taaaatattg ttagaaatcg
    33181 atttgacttc cctgatcgat tcgtcctatt cttatttcat tttactataa ttgatagtgg
    33241 tcgccccagc cagatacctt atctgctatc catttaggaa cccctaactt aagcaatccc
    33301 cataatcgtc tcgatttctt cttccattgc ttccagataa tcactcgtag gcgagtacgc
    33361 aagcgctcat ctatgctagt gactatactt ttcatattta taattcattc ctttcgtttc
    33421 actcaaggca caacacagaa tgaaaaagtg ttgtgatctt tattttgttt tataataata
    33481 gtgagaaagc ctatcactac tacaaatcac ggggaggtga ataagtgagt ggtacagcca
    33541 ctacctcgca tattttgtca catcatttaa cggtacataa taagttgtac catctgaata
    33601 agttgctaca atatcatttg catgctctcc ttcaccttta gcaaaggttg gagctcctgc
    33661 tggatgattt ttatttgcct ctttcaattt ttcaataatg gcattttttt ctgtatcttt
    33721 tatattatca ggatttttca ctaagatttt gtctggatat gtcggtttag cagaaacaat
    33781 ttttactgtt atttcttttt tattcgaagc acttgtccag tttccagcat tatctttagc
    33841 atttaatttt acagtaattc ctgaactagg aacttcagta gcaggttgat tatcaacatt
    33901 attcaacttt aatttcaaaa gagctgttgc atcagacgtt ttatcaatcg ttatatataa
    33961 tgatgaattg ttattataac cagtttcttc atatttagct gtttgtgagc tacttgaaac
    34021 agaactgaaa ttatgcccac tacctccctg attatcttca atgcttacat ctaaatgaac
    34081 ttccccacta ttatttggct tagcaacaac tgttatagta aaataacata aaatttgcat
    34141 aaataaatta ggtaaatcaa agcaacttct aggaatgttt tagcagtcac agtgtacttt
    34201 cccagcatca agccactata actctgcaca taaaaatgga gaagatggca atcctcttct
    34261 ccaaatatta acttctttac aaaccaacta tagttgacaa agaacctaaa atcaattgat
    34321 aacacaaggt caggtcggtc aactctttca actgaagccc tgtcaactct tcccatttat
    34381 caatcttgta ttggagagaa ttgcggtgca gatagagttg ctgggctgtt ttagtgagaa
    34441 cagcactatt ttcccaaaga gagagaatga tttcctgaat ctgatcttga tccaaaatca
    34501 tctggtgtag acattccttg attggcttca agtccacgag tctttctccc atactccaaa
    34561 gatagagctg agaaaaagta tgaacacctt ggtgaccctg acgccaccat gtcttgaaca
    34621 aatcccgctc agctttgatt aagtctgata gggcttgatg tcctgtctga gaccaaacct
    34681 gacccaacat gatagaaaga cgaagtccaa agtcatactc aactgcttca atcatatcac
    34741 ttaaaatatc tcttacagaa gtgtatttgt cttgttgaag cacgaaaaca taatcctgag
    34801 ctccgacctg tagcactgtc tgacaattcg gaaaaagagt ccgcatcata tctagccaag
    34861 aagccagatt ttcctgctga aaataagaaa gatggcaata aaccaactga atctttttaa
    34921 aaacttgcgg tgcctgtccc ttgccttcaa ccagatagga ataccaaggg tttagcgaac
    34981 gagcctgctc ctgctgggtc aaaagggcaa ccaactgctt ttcacgctcg ctgagcccag
    35041 cttcctccag caaaatccac tgctgagaag ctaaagggag cgtgagatag ccctctttct
    35101 ctactggttg gtctgaaatc cgagcctcag gaaaccagtc ttgtagttct tttgccctca
    35161 tgttctagcc ctccactttt tggatgcacc atgaaaccaa actctcaaga cgttccagat
    35221 tctcagtcat atggagatag cccataaccg cttcaaatcc cgtggacata cgataagtca
    35281 cgacatctgc atttttagcc tttgtgtggc tattggtatt gcggccacgt ttgtagattt
    35341 cttcttcttt ttccgttagg acctgctcct ccaacatgag agcaatcagg cgagcctgag
    35401 ccttggctga cacatacttg gttgcttctt gatggagttt attgggtttg gtcatacctt
    35461 tgaggatgag gtgacggcga atatacatag aatacaccgc atccccctca aaggctagcg
    35521 caatcccgtt aatgagattg acatcaatca cgtgtccacc tcactccatc cttggtatca
    35581 aggagcttaa ttccttgagt aaccaattgg tctcggattt ggtctgctgt cgcaaagtca
    35641 cgattggcac gcgcctcttg gcgtttttga atcaaatctt caatctctgc atccaaaact
    35701 tcctcaacaa agacaattcc aaaaatttct aacatatctg caagagcttg cttgacactt
    35761 gcatcatagt tccctgagtt gatccatttg gccatttcaa agacaactgt gataccgttg
    35821 gcagcattaa aatcttcatc catagctgct acaaacttat ctttaaagtt ttgtaactct
    35881 tgggcatcca cgtttcctgt aaatggttgt tcgtaagtat tcttcagata cttgagattg
    35941 gtctcggcat cacgaactgc cttttccgtg aagttgatag gcttacggta gtgctgggtc
    36001 gcaaagaaga aacgaagtac ttgcccatca agagttttaa gggcatcgtg taccgtaatg
    36061 aagttaccca aggacttaga cattttgaca ttgtcgatat tgacaaagcc attgtgcatc
    36121 cagtagttag caaaagcctt gcctgtttta gcttcagatt gggcaatttc attggtgtgg
    36181 tgtggaaact ctaggtcagc tccaccaccg tggatatcaa tggtatcacc taaaatctct
    36241 gtcgacatga ctgaacactc aatatgccaa cccggacgtc caggtcccca aggactatcc
    36301 caagaaatct cacctggttt ggaagatttc catagagcaa aatctacagg attttcctta
    36361 cgagccgttt cttcatcggt acgacctgaa gcacctagat ccaaatcttc caaggtttta
    36421 ttagccaatt tagcatagtt gtgggatttt tctacacgga aatagacatc cccttgactc
    36481 tcataggcaa agcctttctc gatcaagtct tccacaaaac ggatgatgtc tgccataaac
    36541 tccactacac gcggatggcg agtcgcaggt ttcacgccta aggccgtcac atcctcacga
    36601 aaggcagcga tgtacttatc cgcaacctcc tgaggcgtga tgccttcttc cctggcacgg
    36661 ttgataatct tatcatccac atctgtaaaa ttggaaatat aggcaacctt atacccacgg
    36721 tactcaaaat agcgacgaat cgtatcaaaa gctaccgtcg aacgggcgtt tcccacgtgg
    36781 atatagttgt acaccgttgg cccacaaaca tacatcttga tcttgccgtc ctcaatcggg
    36841 acaaattctc gcaaatcacg agacatggtg tcatagattt taatcataaa tcataatcag
    36901 gaaagctgaa atccaagaac aattagtttc atcactaaaa gttcaagtaa atttcagtcc
    36961 gaatatctct acacttcgga atcccttgct cctttctcat tcagataaac cacctgagtc
    37021 tgtttgacaa agccaatttt ttcatacaaa cgtttggcac ctacattgct atcttccact
    37081 gcaatctgaa attccttgtc attttgctca attagttggt tgacgaggga ttttgctaag
    37141 tagcttccat agcctttccc acgttcaggt tccgatattg ctaaaccgta gaagtaattc
    37201 gtattagtcg ataaatctac cgtgcaagtc ccaataacct gaccgtcttt taacaaaata
    37261 tatagtcggc tttctggatc cttcagagct tcagcgacat atttatccac aacttctctc
    37321 gattcatgtt cctctgaaaa tgcctgaaat tttaattgac taatttgatc ctgatacgaa
    37381 ctatctgcta acaaaacttc aatatgggaa acatttgcta acggataagg tcttctatcc
    37441 ttacctaacc aagtttctgt ctcttcatcc tcgattagtc cccagttact ggcaaagtca
    37501 ggatgattct ctaaaaaaat acgttctgtc tgaaaagtga ctgatcgaat ggggaaagaa
    37561 gctgtttctc tctcaaaact agtaaacaat acacgcgcaa tcccctgacg gcgatgacct
    37621 ggatgaacca gtatcgtcac ttctacatct tggtcatctg catagacagt taataaacca
    37681 acaagttcgc ctttttcata ataaaggaaa aaggcgggca tgtttgggtc aaaattaagc
    37741 atgttagaga gatagggatc gcgataggta ccgtcatagt tttggcaaca gttaattact
    37801 tttttcgcct cagatagctc ctcttggctt aacttgtttc ttgcttgaat catataggta
    37861 tcctctacaa accagacgat ctgtgactgg catctttagc ctgctcgagt ttattgacat
    37921 aatactctcg tttttcttcg acttcgtgaa tgacaggctc atctttctta ccatgaagac
    37981 ggacaatctt ggccggaata ccgacaaccg tcacatcact aggtacatct gctacgacaa
    38041 ctgctgcagc accgaccttg gcattttcac caatttccac aggtccgata acttgggcat
    38101 gggctgatat gagggctccc tttcgtacag tcggatggcg tttgccacag tctttccctg
    38161 ttcccccaag agtcactccg tgatagagaa gaacgccttt ttcaacaatc gctgtctctc
    38221 caatcaccag accagaacca tggtcaataa agacacctga atcaatctgg gctcctggat
    38281 gaatctcaat ctgagtccaa aagcgccaaa actgactgtg catacgagct aagagtttga
    38341 agccgtgctt ccagagaaaa tgcgagagac ggtgggccgc caaggccttg acacctggat
    38401 aagtcagcaa aacctccaaa gtggtgcggg ccgctggatc attttctttt acgatatcaa
    38461 tggtttcgcg ccaccacccc atacatttct ccttttctta ttctgaatct tttggtgttt
    38521 ctgtaaattc tttcttaggt ttgtgatcct tgtgatgacg tgggcggtga ggacgctcag
    38581 acttttcacc tttttcatca tgctcaggtt ttggcggacg aggtagaaga gccttcatag
    38641 aggcatcgat acggcctttt tcatcaatct tgataacctt aacatcaact tcatccccga
    38701 tttctaccaa atcctctaca cgattggtac gagtccaagc catctcagag atatgaacaa
    38761 gggcatctgt cttatcaaag aggttaacaa aggcaccaaa tttctcgata cgaacgactt
    38821 tagcacggta aacttcatcc actttggctt cacgaaccaa accagcaata atttctttgg
    38881 cacggttaat agcatcttgg tcactagagt agatagacac atttccttct tcgtctatat
    38941 caatcttaac acctgtttca gcgataatct tgtcgatggt ttctccaccc ttaccgatga
    39001 caatcttaat cttgtccaca tcaatcttga tcgtatcaat tttcggagca gttggagcca
    39061 attctagacg aacttctgga atcgttgctt caatgacatc aaggatttca aaacgcgctt
    39121 tcttggcttg agcaagagcc tccgtcaaga tttctgcagt aatcccttga atcttgatat
    39181 ccatttgaag ggctgtaatc ccatcacgag tacctgcaac cttgaagtcc atatctccaa
    39241 agtgatcttc caaaccttgg atatctgtca atactgtgta gttatttcca tctgagataa
    39301 gtcccatagc aataccagct actggcgcct tgattggcac accaccagcc ataagggcaa
    39361 gagttcccgc acagatagaa gcttgagatg aagaaccgtt tgattccaaa acttctgcta
    39421 ctagacggat agcgtatggg aattcttcca agcttggcaa gacttgagca agagcacgct
    39481 caccaagggc accgtgaccg atttcacgac gacctggcgc accgtaacga cctgtttccc
    39541 ctacagaata ttgagggaag ttatagtggt gcataaagcg tttcttgtac tctggatcca
    39601 aaccatcaat gatttgagtt tctcccatcg gagccaaggt caagactgaa agagcttgag
    39661 tttgcccacg agtaaagaga cctgaaccat gtacacgagg aaggaagtca acaaccgcat
    39721 ccaaaggacg gatttcatcg accttacgac catcaggacg caccttgtct tctgtaatta
    39781 aacgtcgcac ttctgcgtgt tccatttgtt ccaagatttc agccacatca cgcataatac
    39841 ggtcaaattc ttcgtggttc gcatattttt cttcgtaaac ggcagtcact tggtctttca
    39901 ctgcttgagt tgcagcttca cgggccaatt tctcttctac ttgaactgcc ttttggaggt
    39961 cactgttgta ggctgcaatg atttcagctt gcaattcagc atccacgtga agcagttcca
    40021 cttctgcttt ttctttaccg acagcagcaa cgatttcttc ttggaaggca atcaattctt
    40081 tgacagcttc gtgcccttta agaagcgctt ccaacatgat ttcttctgac aattctttgg
    40141 caccagactc taccatgttg atagcgtgct tggttccagc tactgtcaat tcaagaagag
    40201 attgctctgc ttgttcttga cttgggttga tgatgatttg gccatctaca tatcccactt
    40261 gtaccccagc aattggtccg tcaaatggaa tatctgaaat agacagtgcc aaagatgaac
    40321 caaacatagc agccattggt gcagatgcat tttcatcata agaaagcact gtattgatga
    40381 cttggacttc attacggaaa ccttccgcaa acataggacg aatcggacgg tcaatcaaac
    40441 gcgctgtcaa ggtcgcatct gttgaaggac gtccttcacg tttcataaag ccaccaggaa
    40501 acttcccagc cgcatacatt ttttcttcgt agttgacttg gagtgggaaa aaatccccag
    40561 ttgccatttt cttagacata acggcagcag tcaagacagt tgactcaccg taacgtacga
    40621 caacagagcc atttgcttgc ttagcaacct gaccagtctc tacaattaac tcacgacccg
    40681 caaaagtcgt ttgaaacact tgttttgcca ttttaatccc ctttggattg atgaaattat
    40741 acgccttgcc tacaaagatc aagataccaa ggacgtcaaa agcaaagtaa aaataggaaa
    40801 ctgacgaagt cttcgatgaa gacaagacag tttatctttt ttacacagct tttcggccgt
    40861 gttcaattac tcaagatgct aagccgtcaa gcaactcaaa ggaaaatagg aaatcgaacg
    40921 acggagcgac tgctcctagg cagatttatc tttttccaca gagttgtagg cgtgttcagt
    40981 tttcaagata catcattaga aaggtttaat attaaagtat ctaaaccttt cacgctaatc
    41041 gctatcgggc gattagctaa atgctttact aactctttcg tcaaataaca tcgatttgac
    41101 tcactcgtgt cgttaaatct tacagtttaa atgcattgta ttatttaata ccttcatctt
    41161 tgtatcaagt acgtacagaa tctattttat catatttttc ttaaaaagtg aggtctttac
    41221 cattaaaaag gaaccattcc cctcacctga gaagaatggt ttgcttttat tatcctagag
    41281 actggtgatt aaacaaggca tgggttgctt gatggatgta ttttgctgta tcagcattat
    41341 tcatcgtata gagatgcaca ccggcaacat cctgagttac caagtccacg atttggtcca
    41401 ctgcataggc aagtcctgct gctctgagcg actcagggtc atgctcatac ttgtctaaga
    41461 tggctttaaa tttgcgtgga agatggatat tctcacaagt cttcaagagt cggagagcct
    41521 gatttcgatt cagaattggc ataattcctg catgaatggg aacatcaatc ccagccaaga
    41581 tgcacttgtc ctgaaaatca tagaagcgct cattgtcaaa gaagagctga gttacgaggc
    41641 tcgaacagcc tgcatccact ttcttcttaa gattttgaat atctgaaatc tgatttggcg
    41701 aatctggatg cccttctgga tagcaagctc caataatatc aaagtgaggg gcttgttcct
    41761 tgataaactc aatcaagtcg gttgcatagc ggaaatcctt ttgtggttct acgtctggaa
    41821 taatatcccc acgaagagcc aagattttct gaaccccaac tttgtccaag tcagcaatag
    41881 tttcagcaac cttgtcctta gttagataaa tagctggcaa gtgggcaatg gtcggaatcg
    41941 ccaagtcatt ttggataaag tcagccaaac gaaccgtcgt ttccttgata ttaaatttat
    42001 tattgctggc agttacactg ataaaatggg gagccaactc ctgcatatcc tgcaaggctg
    42061 aaataatgtt accattaccc acggctgggt ttggagggaa cacttcaaat gagagtgacg
    42121 gtgtttggcg tgacatatgt aataaccttt tctagttgat ttctttttga acaaccactg
    42181 tatggagaga aatccaatct tacaatttct cacgcgcagc tttagctgct tcaacaaggc
    42241 ggatcaagct ttcttttgtt tctgggatac cacgtgtttt caaaccacag tcagggttga
    42301 tccaaacttt cttgcttggc actttagcaa ggatggcttc gattgtgttg tcgatttcgc
    42361 cttcatttgg tacacgaggt gagtggatat cgtaaacccc aggtcccact tctgtttgga
    42421 agtttttcgc tttgagttcg tccaagattt caaggtttga acggctggct tcaaaggaaa
    42481 taacgtctgc atccatgtta tcgatagctg ggatgatatc tgtaaattct gagtaacaca
    42541 tgtgagtgtg gatttgtgtg tctgacgcta ctgttgagtg taccaagcgg aaggcaggaa
    42601 ttgcccagtc aaggtaatct tcgtaccagt cactacgacg gagtggcaat ttttcacgaa
    42661 gagcagcctc atcaatttgg atgattttca caccagcagc ttcaaggtca agcacttcat
    42721 ccttgatagc aagggcgatt tgaagagtag aatccttgat agagatgtct tcacgtggga
    42781 atgaccagtt gaggatggta acaggtccag tcaacatacc tttaacaggt ttgtttgtac
    42841 ggctttgtgc atagctagac catttaacag tgatagggtt aagacgagtg acatcacccc
    42901 agatgattgg tggtttcacc ccacgcatac cgtatgactg tacccaacca tttttagaga
    42961 agaggtaacc tgacaagttt tgaccgaagt actcaaccat gtcattacgc tcaaattcac
    43021 cgtgaacaag gacatcaaag tcaatatctt cttgccactt gatccattcg tcaatcgttt
    43081 cagcaaggaa agcgtcgtac tctttttgag acaattcacc tttacggtaa gccaaacgtt
    43141 tggcacgaac ttcttttgtt tgagggaatg aaccaatcgt tgttgttgga agagctggaa
    43201 gtttgaaagc ttcttcttgg atagcttcac gttctgcaaa ggctggcaaa cgagtgtaat
    43261 ctgcgtctgt caagccagcg atacgcgcac gaagttcagc attttcacca acacgctcag
    43321 tcgcaaagag ttctttgttg gctgcaagag cttctgaacc ttgaccattg cggatagcat
    43381 ccaagtcacg gatttcatcc aatttttcaa ctgcaaaggc aaagtggttc aagagtgctg
    43441 gttcaaattc ttcattagca gttgtaaatg gcacatgaag aagtgagcaa gagcttgtca
    43501 aaacgatgtt ttcagctggg atttgctcaa gaacagccaa gctcttttcg tagttgttgc
    43561 gccagatgtt tttaccattg acaataccta catagagagt cttgtcagct gggaagccac
    43621 ctttaacgag ttcaagagtt ttcttacctt caacaaagtc aagaccgata gcatctactg
    43681 gcaagtttac aaggtcagcg tatacgtcac gaacatcacc gaaataagtt tgaagcaaga
    43741 cttcaagacc ttttttgtca gccaagagtt tgttgtaaag gttcaagaag agagcttttt
    43801 cttcagctgt caagtctttt acaagagccg cttcatccaa ttggatgcga gtcgcaccaa
    43861 gttcagccaa tttagcaaaa acttcttggt aagcaactac taagctatct acgaagtcgt
    43921 ctgctttcac gccttcttca aagtctgaca attgaaggaa agtgaatgga cctacaagaa
    43981 caggacgagt gttcaatcca agttcttttg cttcttggaa ctcatcgaaa atcttgtgac
    44041 cagccaattt tacttgagtg tctttttcaa atttaggaac gatgtagtgg tagttagtgt
    44101 tgaaccattt cttcattgga agggcgcgaa cgtccccttt ttctccctgg taaccacgtc
    44161 ccaaagcgaa gtagcgctca aggtcagaca agtccaagtt ttgaacggat gcaggcacca
    44221 cgttgaaaag gaaagccgca tctaggaagt tatcatagtg agaaaagtca tttgatggaa
    44281 tttcagtgat gcctttttct ttgacaatgt tccagtgttt agcacgcaag tcttttgctg
    44341 ctgataaaag ttcttcctct gagatttctt ttctaaagta tttttcagtt gtaaatttta
    44401 attcacggaa ttcgcccaaa cgagggaaac cgacgattgt agttgacatg atgtgtcctc
    44461 caaaatttgt tgttgaaact atcttaacag aaaagaaagc gtctgtataa ttgtaaaaaa
    44521 ttagggtttg atatagtttg aaactatata tctgtttcgg acaaaagaaa aagacttgaa
    44581 gcaaacgtct caaatccttt gtatagcttg ttttatagct gtattttagt tagaatgctc
    44641 aaacgcgtta ttagtaattc ttataagtga ctatggcttg ttattagaaa agactataac
    44701 tgattctagt caactttttc cctgttcaag tgggacgatt gctagtgtct ttcctaaact
    44761 ggctaggact tttaagactg tatccaactg aggactagtc tttcctgtct ccatcctagc
    44821 tatgacaggc tggcttattc cactgacttc ttccagcttt ttctgactga ttccttgttc
    44881 atacctagcc tcaatcaact cgctcatgat agccactcgc atatcacttt caaggatttc
    44941 ctccttgcta aagagctcag atggacatcc ttccaattac tcccaatagc actattcttc
    45001 atcacttaac cctctttttt ttacgtctat gtatttttaa aaaaatgagc gaattatgat
    45061 tcgatagatt gatcagcggg tttaaaattg ggactaactt gtttcttaac agattttcct
    45121 tttctaggat aaagcaaatc ctgtttgctt aaccccaact ttccatgatg aatccaatag
    45181 taaatggttg aaattcccac gttaacccct ttagccatca ccatcatttc aggcgaaaat
    45241 ttttggttat gatagtggag aatcttttcc tttagtttct tagtcaagct tgatttcttg
    45301 accgagtgct tgcgattatt ttcataagac tgttgagcgt agtcggcaga ataaatctct
    45361 ttgaagcgcc cttttccaag acattgtcgg actgtcccat acttgatttc agtatggata
    45421 gtttgaggag cttttccaag tagagaggca atttctctat ttgattttcc ttcttttttc
    45481 catcgttcga ttaagcgacg gctatcgatt gtcaaatgtt tgccttttgt agtataattg
    45541 tcttgcattt ctgtgccttt taatcatttc aatcttaaat tggacttttt ttacttgggt
    45601 tgtacttaat ctatgaggaa gacaagaaaa agaatatcaa tcaagtaaag tcacaaagtc
    45661 acattagctc cgagcaacca ttgcaaattg aggtactcac acaatgatta aaacatttct
    45721 ctctgccctt tcggtcattc tctttgttat ccctatcata gcttattctt tttttccatc
    45781 ttctaatctt aacatttggc tatctaccca acctatcttg gcacagattt atgccttccc
    45841 cttagctact gcaactatgg ctgctatttt aagtttctta ttttttttcc tatcttttta
    45901 caagaaaaat aaacaaatac ggttttactc tgacattttg ctcttactat cgctcatatt
    45961 actattattt ggaacagata aaaccctttc ttctgcatca aataagacta aaaccttaaa
    46021 attagtaact tggaacgtcg ctaatcaaat agaagcacaa catattgagc gaatttttag
    46081 ccattttgac gccgatatgg ctatattccc tgaactagct accaatatca gaggtgagca
    46141 agaaaaccag agaatcaaac tattgtttca tcaagttgga ctttctatgg ccaactatga
    46201 tattttcact tctccaccta ccaatagtgg aatagctcct gtgactgtga ttgtcaagaa
    46261 aagttatggt ttctatacag aagctaaaac ttttcataca acacggttcg ggacaattgt
    46321 attacattcg agaaaacaaa atataccaga tatcattgcc ttgcatactg ctcctcctct
    46381 gccaggttta atggaaatct ggaagcaaga cttaaacatc attcataatc aattggcttc
    46441 aaaatatcca aaggctatta ttgcaggtga ttttaatgca actatgcgtc atggagcact
    46501 tgcaaaaata agctctcata gggacgcatt aaatgtactg ccaccttttg aaagaggaac
    46561 ttggaatagc caaagtccaa aactttttaa tgcaacaata gatcatattt tattgcctaa
    46621 aaaccactac tatgttaaag atttagacat tgtaagtttt caaaactctg atcatagatg
    46681 tatttttaca gaaatcacat tttaattatt ttatataaaa tcacccctct agtgttcata
    46741 aactagaggg ggaatttgta tcctactatc gtttaacgca cttctgcatt gactttttct
    46801 tcgagagacg cttggatttt ttccatgtag cgtgcgactt cttcgtccgt taagctgtct
    46861 tctggatttt ggaaggtcaa gctataagcc attgacttca taccaagtcc cagtttttca
    46921 cctgagaaga cgtcaaagag tttgatatct gtcaaacgtt tcacgccggc agcttggata
    46981 gcatctacaa cttcttggtg agtcacttct gccttgagga gaagggcaac gtcacggctg
    47041 actgctggga atttggtgat ttccacaaat ggaacagcag gttggagcgc cccttcgatg
    47101 gctgaaaggt taagctcagc tacatacgtt tctggaatat cgtaagcctt ggcagtgact
    47161 ggatgcactt ggccaaggaa accaagaact tggtcaccga gtgaaatcac ggctgtacga
    47221 cctggatgaa ggctagcgat ttcagatgtt gctgtatagg ttacttggag tcccaaacga
    47281 gtaaataggg cttcaaggat tcccttagca tagaagaaat caactggaac tgctgctgtt
    47341 tggaaatctt tttcagcaac caagcctgtc aaggcaaagg caaagctgtt gatctcattt
    47401 ggaagttctt cttttggatt acctgtttgt tcaaagactt ttccaatctc ataaagggcc
    47461 aagtttttat tcttacgtgc cacgttgtag gcaacggtat caaggatccc tgaaatcata
    47521 ttttgacgga ggactgaacg atccacagtc attggccaca tgagttcagt aaggttactt
    47581 ggttgagctg tgaactcaac tgctttttca ggagttgtca gagcataggt gatgatttct
    47641 gtcaaacctg ctccttcagc aatggtacga acttgacggc ggagtttttg tatcacagtc
    47701 aattcaccag ctgtaccatc gtcttttgga aggctggttg gcaagcggtc atatccatag
    47761 atacgagcga tttcttcaaa gagatcagct tcgattgtga tatcccaacg acgacgtggt
    47821 acgctgactg taaagctgtc tgcatttcca gaaagaccaa agccaagacg acggaagacg
    47881 tcttcgacat cagcataaga cagctcagtt ccgaggacac ggttaacatc agcaagagtt
    47941 gaagaaactt ctacatctga agtatcaagc tcacccgctg aaacgatacc cttacgcacc
    48001 gtcgcacctg caagttccgc aatcaggcta gctgccgcat caagggcttc attaactgtt
    48061 gccacattaa ttcctttttc aaagcgagaa gatgactcag aacgaaggtt caggtgacca
    48121 cttgtcttac ggatagattt gccattgaaa acagcagctt caaggacaac acgactagat
    48181 ttttcagaga tttctgttgc ttgaccaccc atgacacctg caagggctac tggcttgtct
    48241 gcaacagtga tgactaggtc attcacgtcc aagtcacgtt cttcaccgtc caaggtcacc
    48301 aatttttcac cagcacgcgc ttcacgcaca cggatgtcag tcccttcaaa tgtgtccaag
    48361 tcaaaagcat gcataggttg accaaagtag agcaggatgt agtttgtcac gtctacaacg
    48421 ttattgatgg gacggatgcc ttcgttcatg agaaggtttt gcaaccattg tggacttggt
    48481 gcgatagtca cattgtccaa gatacgagct gcatagtaag gcgccttgtc tgtcccaatg
    48541 ctgacagaaa gggcatctgc cgcagcttca ttagtttctg ttagagtaaa ttctttaaag
    48601 ttgactgcct tgtcatagat ggctgccact tcgtgagcca ctccacacat agaaagggca
    48661 tctgcacggt ttggtgtgat ggaaagttcg atgatttcat catccaagtc taggtaagaa
    48721 aagacttcct cacctggcac ggcattttct ggcaagattt ggatgccatc tgcgaattcc
    48781 ttaggcacaa ctgagtcaga aattcccaat tcaccaagtg aacagatcat tccaagtgac
    48841 tccaaaccac ggatttttcc ttttttgatt ttgtagttat cagcgatacg agctcctgga
    48901 agagccacca tgaccttgat cccagcacgc acatttgggg caccacaaac gatctgacgc
    48961 tcttcttcgc caacgttaac ctgacaaaca tggaggtgag tctctggcac atcttcgcaa
    49021 gacaagacct caccgacgac aatttttgag agaccagcag ctggtgatcc gacaccctct
    49081 acctcgattc ctgtagttga cattttttca gccaactctt gtgatggcac atcaatgtcc
    49141 accaattctt ttaaccattt ataagataca agcataattt agttctccag aatgaccatt
    49201 gccacactgg ttcttttcct ttcctatcat ttcaatagaa gaatcctctt cttaccttaa
    49261 tttctttctc agtaaccaat ccgtatctac tttttgacca accataaaat gatgttggct
    49321 aaatttttca aaaccatatc ggttataaaa ggcttgagct tttgtattat gctcccaaac
    49381 acctagccaa gcccaagaaa aactattttt tgtagcaagt tcaagtgcga attcaaacag
    49441 ttgcttacct agtccaaatc cttggaattt ttgtagcaca tagaggcgtt gaatttcaaa
    49501 agcgtcctct aattctctct cagtttgagc acttccccag ttgactttga gaaaaccagc
    49561 tatctcctcc tcatgcataa tgaaataggt ttcagagtca ggatttccca actcagttga
    49621 caaagttttt agactataag cctcttcaaa gtattcctgt aactgctctt ccgtattatc
    49681 atacgcaaag gtttcacgaa aggtttgttt ggcaatttta gccaacacct caacatctgc
    49741 catttctact tttctaatca ttatttaaac tgttctgaga agcggacatc tccttggtag
    49801 aatccacgga tatcgttgat tccataacgg agcatagcta cacgctcttg tccaagacca
    49861 aaggcaaagc cagagtatac agtcacatcg ataccactca tttcaaggac acgtgggtga
    49921 accataccgg cccccataat ttcgatccaa cctgttttct tacatacatt acagccttct
    49981 ccaccacact tgaagcaaga aacatccacc tcaacagatg gctctgtgaa tgggaagtaa
    50041 gatggacgca aacgaatttg acgctcttca ccaaacattt tttggacaat caactgaagc
    50101 gttccttgaa gatcagccat agagatattt ttcccaacta ccaagccttc gatttggtgg
    50161 aattggtgac tgtgggtcgc atcgtccgta tcgcgacgga agacacgccc tggcgagatc
    50221 atcttcaaag gacctttaga aaaatcatgg gcatccatag cacgcgcctg aactggagac
    50281 gtgtgggtac ggagcaagat ttcttcagtg atatagaaag tatcctgcat atcacgagct
    50341 gggtggtctt ttggaaggtt catacgttca aagttatagt agtcttgctc cacttcaaaa
    50401 ccatccacga cttgataacc cataccgatg aagatatctt cgatttcttc actggtttgt
    50461 gtcaaaacgt gacggtgacc agtcgcaact ggacgacctg gaagcgtcac atcgatactc
    50521 tcgctagcca gttgagccgc gactttcttt tcttccaaga gcttagctgt ttcttcaaaa
    50581 gcagctgtca agacatcacg agcttcattg acgtgtttcc cgatgattgg acgcatctca
    50641 gcagaaacat ctttcatccc tttgagaatt tcagtgagcg aacccttttt accaaggaca
    50701 gagacacgca aatcttgcat ctctttttca tttccagcag taatctgctt caagctagcc
    50761 agcgtttctt cgcgaagcgc ttttaattgt tcttcaatag ttgacatatt tcctccatca
    50821 gtctctcgta gataaaaaga aaaccacatg ccaaaaactc cactcggagc gttgacacgc
    50881 ggtaccatcc gttttcatct gacaagtcag accttcattt ctaaatccat gcgcaagtga
    50941 attcacccag ctttcatata gagagcttgc agtcacggct ctcctccctg atatacttcc
    51001 cttgggctac tagtctttca gatttctatt caattaccac ttagtttatc agatttttac
    51061 cattcttgca agacctatct tacttctgct tgttagctta ttcttatcta actttatagc
    51121 ggattgaaat aagatgaaaa caaat
//
LOCUS       DAVLVZ010000006        50778 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000006 DAVLVZ010000000
VERSION     DAVLVZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.006"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(105..1466)
                     /gene="dnaA"
                     /locus_tag="VYN77_000315"
     CDS             complement(105..1466)
                     /gene="dnaA"
                     /locus_tag="VYN77_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1557835.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(1679..2437)
                     /locus_tag="VYN77_000316"
     CDS             complement(1679..2437)
                     /locus_tag="VYN77_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1557836.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNYFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(2495..3676)
                     /locus_tag="VYN77_000317"
     CDS             complement(2495..3676)
                     /locus_tag="VYN77_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1557837.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            3859..4338
                     /gene="rlmH"
                     /locus_tag="VYN77_000318"
     CDS             3859..4338
                     /gene="rlmH"
                     /locus_tag="VYN77_000318"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1557838.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            4382..4455
                     /locus_tag="VYN77_000319"
     tRNA            4382..4455
                     /locus_tag="VYN77_000319"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4416..4418,aa:Arg,seq:tct)
     gene            4620..4745
                     /gene="comC"
                     /locus_tag="VYN77_000320"
     CDS             4620..4745
                     /gene="comC"
                     /locus_tag="VYN77_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1557839.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            4766..6091
                     /locus_tag="VYN77_000321"
     CDS             4766..6091
                     /locus_tag="VYN77_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1557840.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYVFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVMVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            6088..6840
                     /locus_tag="VYN77_000322"
     CDS             6088..6840
                     /locus_tag="VYN77_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1557841.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            6892..6963
                     /locus_tag="VYN77_000323"
     tRNA            6892..6963
                     /locus_tag="VYN77_000323"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6925..6927,aa:Glu,seq:ttc)
     gene            6969..7042
                     /locus_tag="VYN77_000324"
     tRNA            6969..7042
                     /locus_tag="VYN77_000324"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7003..7005,aa:Asn,seq:gtt)
     gene            complement(7083..7625)
                     /locus_tag="VYN77_000325"
     CDS             complement(7083..7625)
                     /locus_tag="VYN77_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV1557842.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSIDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            7753..9364
                     /locus_tag="VYN77_000326"
                     /pseudo
     CDS             7753..9364
                     /locus_tag="VYN77_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(9394..11946)
                     /locus_tag="VYN77_000327"
     CDS             complement(9394..11946)
                     /locus_tag="VYN77_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1557843.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTLKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(12008..13630)
                     /locus_tag="VYN77_000328"
     CDS             complement(12008..13630)
                     /locus_tag="VYN77_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1557844.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEDDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            13836..14861
                     /gene="trpS"
                     /locus_tag="VYN77_000329"
     CDS             13836..14861
                     /gene="trpS"
                     /locus_tag="VYN77_000329"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1557845.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            15013..16491
                     /gene="guaB"
                     /locus_tag="VYN77_000330"
     CDS             15013..16491
                     /gene="guaB"
                     /locus_tag="VYN77_000330"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1557846.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SDNLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(16541..17638)
                     /gene="recF"
                     /locus_tag="VYN77_000331"
     CDS             complement(16541..17638)
                     /gene="recF"
                     /locus_tag="VYN77_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1557847.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(17641..18009)
                     /gene="yaaA"
                     /locus_tag="VYN77_000332"
     CDS             complement(17641..18009)
                     /gene="yaaA"
                     /locus_tag="VYN77_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1557848.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            18168..19418
                     /locus_tag="VYN77_000333"
     CDS             18168..19418
                     /locus_tag="VYN77_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1557849.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILDETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVWKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            19415..20698
                     /locus_tag="VYN77_000334"
     CDS             19415..20698
                     /locus_tag="VYN77_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1557850.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDIIEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            20740..21561
                     /locus_tag="VYN77_000335"
     CDS             20740..21561
                     /locus_tag="VYN77_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1557851.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            21572..22117
                     /gene="pgsA"
                     /locus_tag="VYN77_000336"
     CDS             21572..22117
                     /gene="pgsA"
                     /locus_tag="VYN77_000336"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1557852.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            22114..22941
                     /locus_tag="VYN77_000337"
     CDS             22114..22941
                     /locus_tag="VYN77_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1557853.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            22926..23765
                     /locus_tag="VYN77_000338"
     CDS             22926..23765
                     /locus_tag="VYN77_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1557854.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            23758..24552
                     /locus_tag="VYN77_000339"
     CDS             23758..24552
                     /locus_tag="VYN77_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1557855.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            24610..25428
                     /gene="mreC"
                     /locus_tag="VYN77_000340"
     CDS             24610..25428
                     /gene="mreC"
                     /locus_tag="VYN77_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1557856.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRAYNENESLKKQIYQLEVKSNEAESLKTENEQLRQLL
                     GMKSKLQATKTLAADVIMRSPVSWKQELTLDVGKSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGTTTIYGIIVGYDKENEVLKISQLNSNSDISTGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            25428..25922
                     /gene="mreD"
                     /locus_tag="VYN77_000341"
     CDS             25428..25922
                     /gene="mreD"
                     /locus_tag="VYN77_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1557857.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGIATLLFILLGAFLHKLNSVILLNRW
                     TRILAMIVMSFLFDMGTYLFALVVGLTLDSLPIFIVYSLVPSMILNLVWMLIFQFIFE
                     RYYL"
     gene            26016..27203
                     /locus_tag="VYN77_000342"
     CDS             26016..27203
                     /locus_tag="VYN77_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1557858.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLENEITELSKNIVSRND
                     SLEKQARSAQTNGAATSYINTIVNSKSITEAISRVAAMNEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLSDDAQALTTKQAELKAAELNLAAEKATAEDEKASLL
                     EKKAEAEVAAKVAAEAEAAYKAKQVSQQQTVVASGNTTFAAQVQAVASSESATYTPVA
                     VKQRPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVG
                     AIACWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTLGNHRGWFNPTTTSSGFVTYI
                     YAD"
     gene            27428..28207
                     /gene="rpsB"
                     /locus_tag="VYN77_000343"
     CDS             27428..28207
                     /gene="rpsB"
                     /locus_tag="VYN77_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1557859.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAAAVEAEFAASEAQADSIEEIVEVVEGDNA"
     gene            28286..29326
                     /locus_tag="VYN77_000344"
     CDS             28286..29326
                     /locus_tag="VYN77_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1557860.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALTMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVIEGGDEALAKQISMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKSQLTDEVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            complement(29448..30374)
                     /gene="cysK"
                     /locus_tag="VYN77_000345"
     CDS             complement(29448..30374)
                     /gene="cysK"
                     /locus_tag="VYN77_000345"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1557861.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKTANSNIQVYAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     IAKKLGTGKKVLALAPDNGERYLSTALYEFEV"
     gene            complement(30659..31294)
                     /locus_tag="VYN77_000346"
     CDS             complement(30659..31294)
                     /locus_tag="VYN77_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1557862.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            31350..32648
                     /locus_tag="VYN77_000347"
     CDS             31350..32648
                     /locus_tag="VYN77_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1557863.1"
                     /translation="MKVNLDYLGCLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            32645..33307
                     /locus_tag="VYN77_000348"
     CDS             32645..33307
                     /locus_tag="VYN77_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1557864.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            33387..33935
                     /gene="raiA"
                     /locus_tag="VYN77_000349"
     CDS             33387..33935
                     /gene="raiA"
                     /locus_tag="VYN77_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1557865.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            34071..36044
                     /locus_tag="VYN77_000350"
     CDS             34071..36044
                     /locus_tag="VYN77_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1557866.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            36041..36493
                     /gene="rplI"
                     /locus_tag="VYN77_000351"
     CDS             36041..36493
                     /gene="rplI"
                     /locus_tag="VYN77_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1557867.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            36537..37889
                     /gene="dnaB"
                     /locus_tag="VYN77_000352"
     CDS             36537..37889
                     /gene="dnaB"
                     /locus_tag="VYN77_000352"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1557868.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDHGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            37891..38157
                     /locus_tag="VYN77_000353"
     CDS             37891..38157
                     /locus_tag="VYN77_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557869.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            38246..39592
                     /gene="cbpD"
                     /locus_tag="VYN77_000354"
     CDS             38246..39592
                     /gene="cbpD"
                     /locus_tag="VYN77_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1557870.1"
                     /translation="MKILPFIVRGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      39675..39777
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            39848..40138
                     /locus_tag="VYN77_000355"
     CDS             39848..40138
                     /locus_tag="VYN77_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1557871.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            40101..40859
                     /locus_tag="VYN77_000356"
     CDS             40101..40859
                     /locus_tag="VYN77_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1557872.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            40898..41905
                     /locus_tag="VYN77_000357"
     CDS             40898..41905
                     /locus_tag="VYN77_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1557873.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            41905..42633
                     /locus_tag="VYN77_000358"
     CDS             41905..42633
                     /locus_tag="VYN77_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1557874.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            42752..43210
                     /locus_tag="VYN77_000359"
     CDS             42752..43210
                     /locus_tag="VYN77_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1557875.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRDLLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            43212..45644
                     /locus_tag="VYN77_000360"
     CDS             43212..45644
                     /locus_tag="VYN77_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1557876.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            45965..46618
                     /gene="rr06"
                     /locus_tag="VYN77_000361"
     CDS             45965..46618
                     /gene="rr06"
                     /locus_tag="VYN77_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1557877.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            46615..47955
                     /gene="hk06"
                     /locus_tag="VYN77_000362"
     CDS             46615..47955
                     /gene="hk06"
                     /locus_tag="VYN77_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1557878.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            48038..48565
                     /locus_tag="VYN77_000363"
     CDS             48038..48565
                     /locus_tag="VYN77_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1557879.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            48644..>50778
                     /locus_tag="VYN77_000364"
     CDS             48644..>50778
                     /locus_tag="VYN77_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEV1557880.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSKN
                     NLTVTSSGQDISKKYADEVESHLQSILKDVNKNLKKVQHTQNVDFNKKLSRIKTKYLY
                     GLKEKSEAELTLKTKETKEELTAAFEQFKKDTLKSGKKVAEAEKKAKAQKEEDRRNYP
                     TNTYKTIELEIAEAEVGVAKAELELEFAQAQVQIPQDTEKINAAKSKVEAAKSNVKKL
                     EKIKSDIEKTYLYKLDNSTKETPKSRVRRNSPQVGDSRELKETIDKAKETLSTYMVTR
                     LTKLDPSVFWFADLLMDAKKVVEEYKTKLEDASDKKSVEDLRKEAEGKIESLIVTHQN
                     REKENQPAPQPGGQAGGSMVVPPVTQTPPSTSQSPGQKATEAEKKKLQDLIRQFQEAL
                     NKLDDETKTVPDGAKLTGEAGKAYNETRTYAKEVVDKSKKLLSQTAVTMDELAMQLTK
                     LNDAMSKLKEAKAKLVPEVKPQPENPEPKPQPEGEKPSVPDINQEKEKAKLAIATYMS
                     KILDDIKKHHLKKEKHHQIVALIKDLDKLKKQALSEIDNVNTKVEIENTVHKVFADMD
                     TVVTKFQKGLIQNTPQVPEAPKSPEVPKVSDTPKAPDTPQVPEAPKAPDTPQVPEAPK
                     SPEVPKVPDTPKAPDTPQVPEAPKAPDTPQIPEAPAPETPAPAPEAPKTGWKQENGMW
                     YFYNTDGSMATGWLEYNGSWYYLNANGAMA"
BASE COUNT        16132 a         8234 c        10688 g        15724 t
ORIGIN      
        1 tgtgaaatct gtggaaaaca gcctttaaac aagtcatatc aagtttttta gcttgtttaa
       61 aaaatgtgga taaaaaagat aaaaaactaa aagttatcca caaattattt gattttcttt
      121 ttgattgatt caatttctaa acgtaaatta tcgtcttgat caatcaaaga ttttattttg
      181 gcatgggcat gaatgactgt ggtatgatct tttcccccaa attccttccc aatttttgga
      241 agactattat ctgttagttc tctagataaa tacatggcta cttgacgggc caaaacaata
      301 ttttgaaggc gtctacttcc cttcatttct ttgatactaa caccataaaa gttaccaact
      361 tcagtttgga ttttatcaat tgggatgacg agcatttggc taacatcttg tttgcgggct
      421 ctaatggctt ctgcagcaat atcaatagtg atatccttga ttttttttac tctggcaatt
      481 aaagtgatgt cgttgatggc tccctcaaga tctcgaacat ttgaatcaaa ttgcccagct
      541 aggtattcta gagtatcact ttggaaattg tagcctaaat gttccgtttt actttgtaaa
      601 atggcaatac gtgtttcaaa gtcagggggt gtgatagttt gtgtcaatcc ccaactaaaa
      661 cgcgtgacaa gcctctcctc gagcccttct agatgttttg gactacgatc actcgttagg
      721 acaatctgtt tttgcttgtc atgaagggcg ttaaaggtat tgaaaaattc ttcctgagtt
      781 gcgacttttt ttccgctgag tgactggata tcatcgatta acaaaagatc aagactacga
      841 taggtctttt taaacttttc catttcccca agtcttaggt gatcaagaaa gtcattaata
      901 aagctttcgg cagggatata tttaacacgc gcattaggaa tattttttag aatttcattt
      961 ccaatagcgt ttaataagtg agtcttacca aggcctggtc ctccatagat aaaaagaggg
     1021 ttataggtca gagccaaatc ttcagagaca gctaaagcgg ctgatacagc ccaaacattt
     1081 ccatcccctt gaataaagtt atcaaaggta tacttttctt ttaatcccgt atctgaataa
     1141 ggaatagata ctaactttgg actatagtca taaagagtta aatttgtagc ttcttcaact
     1201 tgtgagctag tcgtatcttg aggtttggtg aaaatatagt ggggagttat ttcagcgtca
     1261 taaatttcaa aaccagctac tacaataata tcttttagtt gtttttccca gaccatttcc
     1321 atttcagagc gaggtagaaa tatagtggca acattttcct ctaccttgat gagttcagct
     1381 tgaatagcat agaaatcata catggatcga gtcagtcttt cttgtgcaaa ttctaatata
     1441 cgattccaaa attgtttttc tttcaatcct ttctcctcct ttactattta aaagtttaat
     1501 actagagata ttttaccata gatagcaaga attttccaca agctgtgaaa aataatccac
     1561 aatgttacta aactttatcc acaggttggg gataaaagaa gaaattattg atttattaag
     1621 ctttttatcg aaaaacatag tggataacct tgtgagataa aaaaataaaa gaacagcctt
     1681 atttcaggct gttgataatt ctactatatt cttcttgatt tgaaaaagaa ataatgattt
     1741 ttccactgtc ttttttagat agtttaattt ctacatctaa tccgagtagt tttcttaact
     1801 gtttttcttc attttgtatg aaataattag ttttttgctg tttcttttgt tttttctctg
     1861 tcagaagagc ttctaacttc cttacagaaa tatcttcttc tataatccgt tgaaagaaat
     1921 agtcttgttg ttccttattt aacccaacta gggaacgcgc atgggcttgt gatagtttgc
     1981 cattttctac ttctgagaga atctgttctg gcaaggaaag taaacgaatg gagttgctga
     2041 tatatggacg agacttgccc atcttatctg caatttcagc atgggtgaat cctttctcta
     2101 cgagagattc ataggcgcgt gcttcttcta ttgggtttaa attttctctc tgtaaatttt
     2161 caataatgga ctggaccatc atctcttggt ctgaaatctg tttaacaaca gctgggatag
     2221 accgtagacc agctaaaagt gaagcccgat agcgtctctc tcctgcaagg atttcataac
     2281 caataacagg agattgacga acaataatcg gttgaatgac cccattttct ttgatagact
     2341 gtgctagttc atctagtttt tctctatcaa attcttttcg gggttgatag ggattttttt
     2401 gtatatctgt gatagaaatc atttcaaatt tttccatgat tctacactaa cacatctttt
     2461 ctcttatgta aagctttctt tacatagatg tcaattaaga ttctaaatca cctgaactct
     2521 tgttaagttt gatagaggta gtttcttctt tcccgttacg atagtaggtt atcttaatgg
     2581 tgtctccgat agaatggttg taaagagcac tttgtaagtc tgttgatgaa gcaatctctt
     2641 tgtcatctac ttttgtaatt acatcgtatt tttcaaggtg accattggca ggcatattac
     2701 tttgtaccga acgaacaact acaccagatg taacattact tggaatattg agtcttctga
     2761 tgtcgcttgt actcacatta gataaattaa ccatctggat tcccaaagct ggacgcgtca
     2821 cttttccgtt tttttctaac tgttcaataa tattgatagc atcatttgca ggaattgcga
     2881 aaccaagacc ttctacagat gttcctccat ttgtagcaat tttacttgag gtaattccga
     2941 taacctgccc ttgaatattg atcagtgggc cgccagagtt acctgggtta atagcagtat
     3001 cagtttggat ggcttttgta gaaatagctt gtccatcttc cgattttaag gatacatttc
     3061 tattgagact ggatacgata ccttgagtta cagtatttgc atattcagaa cctaacgggc
     3121 taccgatggc aatagcagtt tctcctacag ttaacttact agaatcacca aactcagcta
     3181 ctgttgtcac tttttctgaa gagattttta cgacagcaat atcagagaaa gtgtcagctc
     3241 cgacaatttc tccaggtact ttagtcccat ctgacaatcg aatatctact ttgctggcgc
     3301 catttataac gtgattgttg gtgacgatgt aagcttcttt atcattcttt ttataaataa
     3361 ctccagatcc ttcactagag attcgctgag aatctgtgtc agtatcatca ttgccaaata
     3421 cgctattttg tctgtttgcc gaataagtaa taacagaaac aacagcatct tttactttgt
     3481 taacagcctg tgttgttgaa ttttcgttct tataggcagt ttgtgtaata gtactattgt
     3541 tgttagagtt gtttacacta cttttttgag ttagttgagt tattgaaaaa ctacccaagg
     3601 ctccactaaa aaagctaatg acgataacga ctaataattg aaaccatttt ttgtaaaatg
     3661 tttttagatg tttcatattt gcctccatat gtttgaatta ctgaaagtat aaactgacta
     3721 gcttaattat aacttaaaca caaaagtttt acacaaactg tggataactc ttttgaaact
     3781 gtgattttct taattgaaat ctatttttta ttttgtgaat aagatgtgaa aaaatagaga
     3841 atatgttaga atagagtcat gaaaattaaa gttgtaacag ttgggaaact gaaagaaaag
     3901 tatttaaaag atggtatcgc agagtattca aaacgaattt ctagatttgc taagtttgaa
     3961 atgattgagt tatcagatga aaaaacacca gataaggcca gtgaatcaga aaatcaaaag
     4021 attttagaaa tagaaggtca gagaatttta tcaaaaattg ctgaccgtga tttcgttatt
     4081 gtgttagcca ttgaagggaa aactttcttc tcagaagaat ttagtaagca gttagaagaa
     4141 acttctataa aaggattttc tactcttact tttattattg ggggaagttt aggattatca
     4201 tcatctgtaa aaaatagagc caatctttct gtcagttttg gtcgcctaac cttgcctcat
     4261 cagttaatga gactagttct tgttgaacaa atctatcgcg cttttacgat tcagcaggga
     4321 ttcccctacc ataaatagaa aattgacttt taattgaatt tttggtagaa taattgtgtt
     4381 aggtctcata gctcagctgg atagagcatt cgccttctaa gcgaacggtc gcaggttcga
     4441 atcctgctgg gatcaatata atagcaaagc tgggaatttt cctggctttt ttcttaaaaa
     4501 agtacacttt gggagaaaaa aatgacagtt gagagaattt tatctaaaac gaaattccat
     4561 tttgtataat ggtttttgta agttagctta caagaaaaaa cattttagga gattttatta
     4621 tgaaaaacac agttaaattg gaacagtttg tagctttgaa ggaaaaagac ttacaaaaga
     4681 ttaaaggtgg ggagatgagg atttcaagaa tcatccttga ttttcttttc ctacgaaaaa
     4741 agtaatgaaa taaggagaaa gagtaatgga tttacttgga tttgggacag ttattgttca
     4801 ttttttaatt attagtcaca gttaccgttt aatttgtaaa ggtcgaataa atagaaaaga
     4861 attatacgtt tttggtgctt atacattact aactgaaata gtacttgaat tttcctttta
     4921 tcttctatat ttagataaaa tagggattga aagattttta tttcctttgg gcttatattc
     4981 ctattttcga tggatgaaac agtatgagag ggatagagga ctattcctaa gtttactact
     5041 atctctttta tatgagagca ctcataactt tctgtccgta actttctcct ctataacagg
     5101 agataatttt gttttacaat atcatttccc attctttttc gttgtgacgg tgttaaccta
     5161 ttttgttaca ttaaaaatca tttattattt ccatttggaa ctagcctatt ttgacaagga
     5221 ctacctttat cctttcttga aaaaagtatt ttttgcttta ctattgctac atattgtatc
     5281 tttcgtttca gatatggtaa gtacgattaa acatttgaat agttttggaa gtattttgtc
     5341 atctattgtc tttatctctc tccttttgac cttctttgca atgaattctc ataaagttca
     5401 aatggagaaa gagattgctt tgaagcagaa gaaatttgaa cagaaacatt tacagaatta
     5461 cacagatgaa attgttggtc tgtataatga aatccgtggt tttcgacatg attatgctgg
     5521 aatgcttgtc agcatgcaga tggcaattga cagtggtaat ttacaggaaa ttgacagaat
     5581 ttacaatgaa gttttagtca aagcaaatca taaattgcgt tcagataagt acacttactt
     5641 tgatttgaac aacatagaag actcagcttt acgaagtttg gttgctcagt caattgtcta
     5701 tgctcgaaat aatggtgtag agtttacact ggaagtaaaa gatacgatta ccaagcttcc
     5761 aattgaacta ttggatttgg ttcgtatcat gagcgtttta ttgaataatg ctgtcgaagg
     5821 atcggctgat agctataaaa agcagatgga agtagcagtt attaagatgg aaactgaaac
     5881 agttatggtg attcagaatt catgtaaaat gacgatgact ccttcaggag atctatttgc
     5941 cttaggattc tccactaagg gaagaaatcg cggagtcgga ttaaataatg tgaaagaact
     6001 actagataag tacaacaata ttattttaga aacagagatg gaaggcagta catttagaca
     6061 aatcattaga tttaagaggg aatttgaatg aaagttttaa ttttagaaga tgttattgaa
     6121 catcaagtga gactagagag aatattggat gaaatttcga aagaatcgaa tattccaata
     6181 tcatacaaga caacgggaaa agtccgtgaa tttgaagaat acattgaaaa tgatgaagta
     6241 aatcagcttt atttcctaga tatcgatatt catggaattg agaaaaaggg atttgaagtg
     6301 gctcagctca ttcgtcatta caatccttac gctattatcg tctttatcac tagtcgatca
     6361 gagtttgcga ctctaaccta taaataccag gtatcagccc tagattttgt tgataaggat
     6421 atcaatgatg agatgtttaa gaagagaatt gagcaaaata tcttctacac gaagagtatg
     6481 ttacttgaaa atgaagatgt tgtagattat ttcgactaca attacaaggg aaatgattta
     6541 aaaattcctt accatgatat tttgtatatt gaaacaacag gggtatctca taaattgcgc
     6601 attattggta agaattttgc aaaagagttt tatggtacca tgacagatat tcaggaaaag
     6661 gacaaacata ctcagcgatt ttattctcct cacaagtcat ttttggtaaa tataggcaat
     6721 atcagagaaa ttgatcgaaa aaacttagaa attgttttct atgaagacca tcgttgtcct
     6781 atttcaagat taaaaattag aaaattaaaa gatattttag agaaaaaatc tcaaaagtga
     6841 ttgacaatta gcaagaaatt gatataatgg ttatatctgc tacagataga aggtccgttg
     6901 gtcaaggggt taagacaccg ccttttcacg gcggtaacac gggttcgaat cccgtacgga
     6961 ctattttatc cgccatagct cagttggtag tagcgcatga ctgttaatca tgatgtcgta
     7021 ggttcgagtc ctactggcgg agtatagata aaagggaaca cagctgtgtt cctctttttg
     7081 tatcaatttg tatcaccaag cattttcata aggaagtctg ttatttcttg agaactttct
     7141 ttttttccat gtgcaatcca agtttggcag acaccaaaaa gtgcatgagt tagatagatg
     7201 ctactatatt ctaattcagt ggtatttaga ttcagttgca taaatcgctt ttgtaaatct
     7261 atactgagca tgatatgaag tttatttcgt aagaaatttt ggatttcttt agtcccattt
     7321 tcagaaagaa gggcagccag aagtggttct gactctagat attcaaagac ttctaaaata
     7381 gcgtctcttt tgtgatgagc atgtttttga aaaatatatt caaatgtatg gaatagtttg
     7441 ctttgatagt gctcaatcat atcatactta tccttatagt gagtatagaa gctggaacga
     7501 ctaattccgg ctttttctac taatttgaca gtagaaattt tatcaaatgg ctgttccatc
     7561 agtaattgta ccatagcatt ttcaatagtt cgctttgttt ttaagcgttt gttactttct
     7621 tgcatatttc ttccttgtaa acaaattaga ctatatgtct aaaaatagat tttttatctt
     7681 gtaatttaga ttttttaatg tataatctat tatatcaaaa ttttagacaa tatgtttaaa
     7741 aaaggagaaa ctatgtttaa agaatggaaa gcaatttaaa aaaacaacct ttattattgt
     7801 catgatcggg atttctctta ttccagctct gtacaatatc atatttttgt cattaatgtg
     7861 ggatccatat gggcaattgt ctgacttacc tgtggcagtt gtaaataatg ataaagaggc
     7921 ttcctataat ggtaatacta tggcaatagg aaaagacatg gtgtccaatt taaaagaaaa
     7981 taaaaccttg gattttcatt ttgtagatga agaggaagga aagaagggat tggaagatgg
     8041 cgattactat atggtagtga ctttaccaag tgatttatct aaaaaaacaa ctacattatc
     8101 caatattcaa tcgacagcag cttatcaatc attgacaagt gagcaacaaa ctgggataag
     8161 tgattctgta tctcaaaatt caactgatag tattcaatcg gctcagtcaa ttgtagcttt
     8221 agtacaaggt ttacagggaa gtttagaaaa cttacaaaat caatcttcta atctttcgac
     8281 tttaaaaaat caagctaatc aagtattacc tattacttct acttctttga taggattgtc
     8341 aagtggatta acagagatac aaggagctgt tactagcaaa ttagttcctg ccagtcagtt
     8401 gattgcatca ggtgtaaacg catatactac aggtgttgat aaagtttctc agggcgcaag
     8461 tcaactaagt gaaaaaaatg ccaccttgac acaaaaatct tctagattga cagcaggagt
     8521 tgataaatta caatcaggat ctgggcaatt agcagacaaa tccagtcagt tactttcagg
     8581 tgcttcttca ttagagaatg gagctaataa attggcagat ggatctggga aactagcaga
     8641 aggtggaaca aagttaactt ctggattgga agatttacag acaggacttg cttctttagg
     8701 acaaggacta ggtaatgcta gtgatcaact caaatcagta tcaacagaat ctaaaaatgc
     8761 agagattttg tcaaatccac tcaatctttc aaaaagcaga caatgatcaa gttcttgtaa
     8821 atggaatcgc aatagctcct tatatgatat cagttgctct ttttgttgca gcaatatcaa
     8881 caaatatgat attttcgaaa ttgccttcag gacgtcatcc agagatccgt tgggcttggt
     8941 tgaaatctcg agctgaaata aatggtatta tagctgtttt ggcaggaatt ttggtatatg
     9001 gaggagttca tcttattggt ttaactgcta atcatgagat gagaatattt attctcatca
     9061 tcctaacaag tttagtattc atgtctatgg tgaccacttt aacaacgtgg aatagccgta
     9121 taggagcttt cttctcactt attttgcttt tactacagtt agcatcaagt gcaggtactt
     9181 atccacttgc tttgacaaat gatttcttta aatctattaa tccctggtta ccaatgagct
     9241 attcagtttc gggattacga caaacaatct ccatcaacaa gtcattttcc tagctgtcat
     9301 actagctcta tttactggtt taggtatact agcctatcaa cataagaaaa tggaagaaga
     9361 ttaaaaaaat cgaccgatta actggtcgat tttttatgcc ttagatgact ttcgtctgtg
     9421 attatagatt ccaaatagta agagagaagt aaaggaacag attgctccag taataaaacc
     9481 attgggaatg aaggaaagtg taatagttcc tttccccttg ggaatgtcaa ctttcataaa
     9541 tccagtttga gcttgtttaa tttctatttt cttaccatct tggtaggcag accaaccttt
     9601 gtcataagga atggtgaaga aaatagatgt atcttgttgg acatcatatg tagcaaaaac
     9661 cttgttttta gaagttgata ctgtgacagg ttgttcttta attttttgaa ttgcctcggt
     9721 gaaagttttg gtatctaaac gatagaaggt aggagattca aatgatactt gtgaatttcc
     9781 agggaaacta acattgatat tgaaagtttt tttctcttta gtatatccta gattaaagaa
     9841 ggagaagaca ttatcagttg taaaagtctt tttttcacca tttacaagga tgtcaacctt
     9901 cttttgttta tcgttagaaa agtgaaggtt tatgaaagag agataaactt ggctgttttc
     9961 tggaacttca atttgatact ggattgctgc atcttcattt gaagaacttg tgacactaat
    10021 caaatcatta gtattttcta ttttttctgt tttttcataa ggtattggag aaaaataatc
    10081 aaaattgacg ttagcaagtt gatttaaaaa tgaggcctga ttatccaagg tatgttcatt
    10141 gaacttgaca tcattgtaaa cagattgact agcaactgca atcggaagag agtattgatt
    10201 ttcatatagg gtaagattat ctttttgata gatatcttta aagccatact tatcaatagg
    10261 actgtctgag atattgtact ggataccaaa taaactatca gccaaaatac tattatttgc
    10321 atatcggaga ttgagattag tcccagagga tttaaaacca agtttatcta aagtagagct
    10381 tgatgaacga tttcgaacag atgaaaattg agagattcca ttgtagttga atttcatact
    10441 gtcatttcct gtctgagttt gtagtttttc agtacgagta aattgatttc caatatatgt
    10501 tgagaaagat tccatagctg ggatatctcg actataagca cttcgagaag caaatcccca
    10561 ttccttagca attccgtcca tttgagatga agcatttaaa ctcatttcaa ccagtataaa
    10621 taaagagatt agaatggcaa atagattcac agatataaac tttttgataa ctgcaaggag
    10681 taaaagagaa tagacaacca aaaattcaag agtaagcaga atattcaaat ctgttaaaaa
    10741 agaataatgc gattttagat agatggtagc taaaaatcct gttactacaa gaaaaagcga
    10801 aactaaaaaa ttccagactt taagttcttt cagacgcttt aagacttctg ctgctgtgta
    10861 aattaacaag gtagagaaaa tccaagcata gcgatgtaaa aacatgtttg gagtatgcat
    10921 gccttgccaa aataagtcaa gagcttctat gtaaaagctt gcaattagaa atgcaaagaa
    10981 tattacatat atgagtttca cgtgaaactt aatagatttc agcgtaaaaa ataaaatggt
    11041 caaaataaag ggaaatagtc caacaaaaat cattgggatg gccccatact ttgttgtgtc
    11101 aaaggaacca atgaattgct tagcaaagag atcaagatac cagctacttt cagtttgaaa
    11161 ctttgtaact tcagtcaatt tttccccatg tgtctgtaaa tcaaatagag tgggaagagt
    11221 cataatcaaa ctagccatac cagctaaaaa ggagataact atgaaatcaa gaacagatga
    11281 ttttagagtc ttaaagtccc acgaaatttg acagagatac cagaaaataa gaaacaatac
    11341 tgtcatatat ccaaaataat aattttgaat aaataagatt gacagacttg taaagtacaa
    11401 taggagtttc ttttcagtta tcagtagatg taaaccagtt ataattaaag gaatcaagat
    11461 aaaaacatct agccaggttt ttatctctaa ttgactgaca gtgaaactca tcagagcata
    11521 ggaagtagat aaggctagtt ttaaagtctg agggatagat tgaaacaatt tattcaaact
    11581 aaaaaaggtt gacagaccaa tcaatccaaa ttttaagaga gtggtcagat agacagcatc
    11641 tggcatattc gttagatcaa aaaagtaaac tagaggcgcg agaaaactac ccaagtaata
    11701 actagatagg gcatagaagt ttagccctag accacttgta aaggtgtaaa acagactact
    11761 atttccatgt aggatatttc gtaaggctac atcaaaaata acgtattgat gaaagccatc
    11821 tcctaataga ggagagttgt cgctattcca gtagatacct tgagatagat atactccaga
    11881 cataatcact acaggaatga tgaaagaaat aaaataggtt cgatatgttt ttaaaaatga
    11941 tttcatgtta cctcgtagaa tgatagaaaa ctcagttggt taacccaact gagttttgaa
    12001 gttttattta gtctttccaa agttctttaa cttttgcttg tacttctgca ttttctagga
    12061 attcatcgta ggtttcatcg atacggtcaa tgacgccatt tttagataag acaatgatat
    12121 ggttagccaa agtttgaata aactcgtggt catggctggc aaagatgatt gattctttaa
    12181 agtttttcaa tccatcattc aagcttgaga tagattccaa gtccaagtga tttgttggat
    12241 catcaagtac aaggacattt gattttaaga gcatgagttt tgaaagcatg acacgaactt
    12301 tttctccccc tgacaagaca tttacaggtt tgttaacttc atctccagag aagagcatac
    12361 ggccgaggaa accacgtagg aaagtattgt catcatcttc tttacttgcg aattgacgca
    12421 accagtcaag aattgattct cctcctgcaa aatcagctga gttatctttt ggtaggtaag
    12481 attgactagt tgtaactccc cacttgacag ttccttcata gtcaatatct cccatgattg
    12541 cacgaattaa tgcagtcgtt tgaatatcat tttgtccaat aagtgctgtc ttatcatctg
    12601 gacgcaagat gaaactaata ttatccaaga tagtttcacc atcaatcttt acagttagat
    12661 tttctactgt caagagatca ttaccaatct cacgttccgc tttaaagttg ataaatggat
    12721 atttacgact agatggtaca atctcttcta gctcaatctt atcaagcatt ttcttacgtg
    12781 atgttgcctg ccttgactta gaagcattgg cagagaaacg agcaacgaat tcttgcaatt
    12841 gtttaatttt ttcttctgct ttagcattac ggtctgctag caatttagca gcaagctcag
    12901 aagattcctt ccagaagtcg tagtttccga catagagttt gatttttcca aagtcaaggt
    12961 cggccatgtg agtacaaact ttgtttaaga agtgacggtc gtgggatact acgataactg
    13021 tgttatcaaa gtcaattaag aagtcttcta accaagtaat cgattggata tccaaaccgt
    13081 tagtaggctc gtccaagaga agaacatctg gtttaccaaa aagtgctttg gcgaggagaa
    13141 cctttacttt ttcaccgttg gccaattcgc tcatgttttg gtagtgtaat tcttctggaa
    13201 tgtttaggtt ttgaagtagt tgagaggctt cactctctgc ttcccaacct ccaagttcgg
    13261 caaactctcc ttcgagttcg gcagcacgaa ccccgtcctc gtctgagaaa tcttccttca
    13321 tgtagatagc atctttctct ttcatgatgc tataaagttt ttcatttccc atgataacga
    13381 catcaatggc acgttcatct tcgtagtcaa agtgattttg acgaagaaca gagagacgtt
    13441 catctggacc aagagagatg tgaccagtag taggttcgat atctccagct aaaattttta
    13501 aaaaggttga ttttccggca ccattagcac cgattaatcc gtaagtattt ccttctgtaa
    13561 atttgatatt gacatcatca aaaagtttgc gatcactaaa acgtagtgaa acatcagata
    13621 ctgtaagcaa tatttttctc ctatatgtgt aatatattta ttctactaga aaatacagaa
    13681 atattcaaat ttttatttgt caattttgtg taaattatat ttacagtatc ctttacacaa
    13741 atctgtaaaa agcaaggctg atttattttg ataaattacg gttatttcat taaaaaaatg
    13801 ctataattga aaggactata tcgaaggaga acaaaatgac taaacccatt attttaacag
    13861 gagaccgtcc aacaggaaaa ttgcatattg gacattatgt tggaagtctc aaaaatcgag
    13921 tattattaca ggaagaggat aagtatgata tgtttgtgtt cttggctgac caacaagcct
    13981 tgacagatca tgccaaagat cctcaaacca ttgtagagtc tatcggaaat gtggctttgg
    14041 attatcttgc agttggattg gatccaaata agtcaactat ttttattcaa agccagattc
    14101 cagagttggc tgagttgtct atgtattata tgaatctagt ttcgttagca cgtttggagc
    14161 gaaatccaac agtcaagaca gaaatttctc agaaaggatt tggagaaagc attccgacag
    14221 gattcttggt ctatccaatc gctcaagcag ctgatatcac agctttcaag gctaattatg
    14281 ttcctgttgg gacagatcag aaaccaatga ttgagcaaac tcgtgaaatt gttcgttctt
    14341 ttaacaatgc atataactgt gatgtcttgg tagagccgga aggtatttat ccagaaaatg
    14401 agagagcagg gcgtttgcct ggtttagatg gaaatgctaa aatgtctaaa tcactaaata
    14461 atggtattta tttagctgat gatgcggata ctttgcgtaa aaaagtaatg agtatgtata
    14521 cagatccaga tcatatccgc gttgaggatc caggtaagat tgagggaaat atggttttcc
    14581 attatctaga tgtttttggt cgtccagaag atgctcaaga aattgctgat atgaaagaac
    14641 gttatcaacg aggtggtctt ggtgatgtga agaccaagcg ttatctactt gaaatattag
    14701 aacgtgaact gggtcctatt cgtgagcgcc gtattgaatt tgctaaggat atgggagaag
    14761 tttataatat gattcaaaaa ggtagtgaaa gagcgcgtga agttgcgggt caaaccctat
    14821 ctgaggtaaa aggggcaatg ggacttcatt actttaacta agtttatttt acaagaaact
    14881 atcatttcta tctcgaagaa aagatagttt tttatttacc cctgtaacat ttgacaaatt
    14941 tttatagaat ggtatgatag atacaatata aaaagagtca agctcaaaaa gaaagaaaag
    15001 aggaaacttc gaatgtctaa ttgggacact aaatttttga aaaaaggttt tacctttgat
    15061 gatgtattgc ttattccagc tgaaagtcat gtgttgccta acgatgcaga tttaacaact
    15121 aaattggcag ataatttgac tttaaatatc ccaattatta ccgctgccat ggacacagtt
    15181 acagagagtc aaatggccat tgctattgct cgtgcaggtg gtctcggagt tatccataaa
    15241 aacatgtcaa ttgctcaaca agcagacgag gttcgtaagg taaaacgttc tgaaaatgga
    15301 gttattattg atccgttctt cttgacgcct gaacatacaa ttgctgaagc agatgagctt
    15361 atgggtcgtt accgcatcag tggtgttcca gttgttgaaa cacttgaaaa tcgtaaattg
    15421 gttggtattt tgacaaaccg agatcttcgt tttatttcag attataatca accaatttca
    15481 aaccatatga ctagtgacaa tcttgttact gctcctgtgg gtacggatct tgcaacggct
    15541 gagagtattc ttcaagagca tcgtattgaa aaacttccgt tggtcgatga agaaggcagt
    15601 ctttctggtt tgatcactat caaagatatt gaaaaagtta ttgagtttcc aaatgcggct
    15661 aaagatgagt ttggtcgtct tctagttgca ggtgcagtag gtgttacttc agatacattt
    15721 gaacgtgcag aggctctttt tgaggcagga gcggatgcga ttgttattga tactgcacat
    15781 ggtcattctg caggtgtctt gcgtaaaatt gccgagattc gtgctcattt cccagatcgg
    15841 actttgattg ctggaaatat tgctactgct gaaggtgcac gtgcccttta tgaagcgggt
    15901 gtagacgttg ttaaggttgg tattggacca ggttctatct gtactactcg tgtgattgct
    15961 ggtgttggtg ttccgcaagt aacagctatc tacgatgctg cagctgttgc gcgcgaatat
    16021 ggtaaaacga ttattgctga cggtgggatc aagtattctg gagatattgt aaaagcactt
    16081 gctgcaggtg gaaatgctgt tatgcttgga tctatgtttg ctggaactga tgaagctcca
    16141 ggcgaaactg aaatcttcca aggacgtaaa ttcaagactt accgtggtat gggatcaatt
    16201 gctgctatga agaaaggttc aagcgaccgt tatttccaag gttctgtcaa tgaagcaaac
    16261 aagcttgttc cagaaggaat tgaaggtcgt gttgcttata aaggagcggc agctgatatt
    16321 gttttccaaa tgattggtgg tattcgctct ggtatgggtt actgtggtgc agctaacctt
    16381 aaagaactac acgataatgc tcaatttatt gaaatgtctg gtgctggttt gaaagaaagc
    16441 catcctcatg atgtgcaaat tactaatgag gcaccaaatt attctatgta aaaacaatga
    16501 aaagaactcc agtgaaaaca ggagttcttt tacaatgttg tcaatttcca tttacagcag
    16561 ctttaccatc ctgaatagtg aagatactta gattttctgg cagattttga agatggtcta
    16621 agcttgttgt tgtgataaag gtttggattg attgagaaat cgtttctaat aattttaact
    16681 gtctagtgtt gtcaagttca ctcatcacat cgtcaagcag taatatcgga gattctgtgg
    16741 taatgctttc cattaattcg atttctgcta attttatcga gaggacgaga ctacgatgtt
    16801 gaccttggct tccgaaacta gcatccatcc catttatata aaaagaaatg tcatctcgat
    16861 gaggaccgac accagtattc tttttaaata aatctctgga tctacttttt tctaaagcaa
    16921 ttttgaaaga ttcggataag ttttgtttgt cagttatatt gacagaagat tgataggata
    16981 ttgacaactc ttcgatctga ttagagagtt caaaatgttt cttacgccca aatgattcta
    17041 gtttttttat gaaatctaag cggtgattca ttacacgaca tccataatca actagctgat
    17101 catctaacac agaaaggaat gtttcatcta ttttttgagc tgattttagg taagtgtttc
    17161 tttgctttag gatgtggtta taattggtta agtcagataa atagattggc ttaatttgcc
    17221 caagttccat atcaatgaat tttcgtcgaa tcgaaggtgc tcctttaatt agttgtaaat
    17281 cttcaggagc aaataagaca acattcatgt gtcctacata atctgaaagg cgtgcctgtt
    17341 ttaagtgatt aacttttgtt acacggcctt tttgtgttag ttcgatttct aggggaatgg
    17401 atccagtttt tttctgaacg agacctgaaa gatgaagttg ttcctcatca aaatgaatga
    17461 gatttttatc tgttcgagtt cgatgactac gcgttaaggc taaaaaatag atagcctcta
    17521 acatgtttgt ttttccttgt gcattacgtc ccaaaaagac attcaattta ggattaaagt
    17581 ctattttcgt ttctttgtaa ttacgaaaag tcttgagaga tagatgttgt agccacatga
    17641 ttatctacca gggaatcgtg gagcttgttg gcttttaggt gatgaagcag gtttcgattt
    17701 gtctttttta actcccttat tcatcttttt gacaagttta gcgatccgtt ctttttcaac
    17761 tttatcagct tggtattcat cttgttcttc agaagtaggt tgtgtcaaca agatgtcaat
    17821 gttcatgtca gggatgtcaa ctttatcgcc aatacgaagt ttttttccac gacgactctc
    17881 taattcccta ttaaagtaaa cagaatgttc agagagaaat gatttaatag ctcctccgct
    17941 atgtgtaatt ccaagttctt tgagtagtgc ttggagggta ataaattctt caaataattt
    18001 gtattccata attcacctca atctttggta ttataccata ttttcctaaa aatagtgaga
    18061 gagacagcga gaaatgtaag cgatttttat ttcaaaagtg ttacagggaa caagaaaatt
    18121 tcaggttttc gtgatataat agaagtctgt atataaggag gtaaatcatg gagttagtgc
    18181 atggaatttc aacacatttt atccaatcaa aaaagtttaa aacaaacaaa attaccgtgc
    18241 gttttaccgc tccattatcc cttgatacga ttgcaggtca catgttgagt gcaagtatgc
    18301 tagagactgc taatcagatg taccccactt ctcaagattt gaggagacac ttggccagtc
    18361 tatacggtac agatatgtca accaattgtt tcagaagagg gcaaagccac attatagaat
    18421 tgacatttac ctatgttcgt gatgagtttt taagtaggaa aaacgtgcta acttctcaga
    18481 ttttggaact tgtaaaagaa actctttttt cacccgtagt agttgataat gggtttgatc
    18541 cggccttatt tgaaattgag aaaaaacaat tgctagcaag tttagcagct gatatggatg
    18601 attcttttta ttttgcacat aaagaattgg ataaattgtt ttttcatgat gaacgtcttc
    18661 aattggaata tagtgattta cgaaatcgta ttttagatga aactccacaa agttcttatt
    18721 cttgtttcca agaattttta gccaatgatc gaatagattt ctttttccta ggtgatttta
    18781 atgaggttga aattcaaaat gtattagaat catttggctt taaaggtcga aaaggagatg
    18841 tgaaggttca gtattgtcaa ccttattcta atatccttca ggaaggtatg gtttggaaaa
    18901 atgtgggaca atccattttg gaattaggtt atcattactg ttctaaatat ggtgatgagc
    18961 aacatttacc catgattgta atgaatggtt tacttggtgg atttgctcac tctaagctct
    19021 ttacaaatgt ccgtgaaaat gctggattag cttataccat ttcaagtgag cttgatttat
    19081 ttagtggatt cttgaggatg tatgctggta tcaatcgaga aaatcgtaac caggctcgta
    19141 aaatgatgaa taatcaactg cttgatttaa aaaaaggtta ttttacagag tttgagttaa
    19201 atcagaccaa ggaaatgatt cgttggtcgt tgttactttc tcaagataat caatcttcat
    19261 tgattgaacg tgcttatcaa aatgccttat ttggaaaatc ttcagcagac tttaaaagtt
    19321 ggattgcaaa gcttgaacaa attgacaaag atgctatttg tagagtagct aataatgtga
    19381 aactacaagc gatttacttt atggaaggaa tagaatgaca aaggttgttt ttgaagaaaa
    19441 atactatcca gctgtaaaag aaaaggttta tcgaactcgt ttggccaacg gattgacagt
    19501 tgctcttttg cctaaaaagg aatttaaaga ggtttacggg agtgtcactg tacagtttgg
    19561 ttcggtagat acgtttgtca cagaagttga cggagatgta aaacaatatc ctggaggaat
    19621 tgctcatttt cttgaacata aattatttga gagagaagat tctagtgatt tgatgtcggc
    19681 ttttacgagt ctaggtgcag atagtaatgc ctttacaagc tttacaaaaa caaactatct
    19741 tttttcagca acggattatt ttttagaaaa tttagattta cttgatgaat tggtaacatc
    19801 agcacacttt actgaagctt ccattctgac agagcaggat attattcagc aagaacgaga
    19861 aatgtaccaa gatgatccag attcgtgttt attcttttca actttagcga atttgtatcc
    19921 tggtacacct ttagcaactg atatagttgg aagtgaggag tccatttccc aaatcaatct
    19981 aactaatttg caagaaaatt ttacaaagtt ttacaaacct gtaaacatgt ctctgttttt
    20041 agttggtaat tttgatgtgg agcgagtaca ggactatttt gaaagcaaag aactgaaaga
    20101 ttcagatttt caggaagtag caagagaaaa gttgttttta cagcctgtaa agccaacaga
    20161 tagtatgaga atggaagtat cttctcccaa actagcgatt ggagttagag gtaagcgaga
    20221 agtttctgaa gcggattgct atcgacatca tattttatta aaattattgt ttgcaatgat
    20281 gtttggttgg acttcggatc gttttcaaaa atgttatgaa tcaggtaaaa ttgatgcatc
    20341 cttatctctg gaagttgaag taacaagtcg ctttcatttt gtcatgttga caatggatac
    20401 gaaagagcca gttgctttgt ctcatcaatt taggaaggct attcgtaatt ttacaaagga
    20461 tttagatatt atagaggaac atttagatat tatcaaaaga gagatgtttg gcgaattttt
    20521 cagtagcatg aactctcttg aatttattgc aacgcaatat gatgcttttg aaaatggtga
    20581 gacaattttt gatttgccga aaattttaca ggaaattact ttagaggatg tccttgatgc
    20641 tggacatcat ttaatagatg atggtgacat agttgatttt acaatattcc catcgtagta
    20701 acctattata atagacacta gaaagaaggg atgacaagta tgagaaaaaa aacaattgga
    20761 gaggttttac gattagctag aatcaatcag ggattgagtt tagatgaatt gcagaaaaag
    20821 acagaaatcc agttagatat gttggaagca atggaagcag acgatttcga tcaacttcca
    20881 agtccttttt acacgcgttc tttcttgaaa aaatatgcat gggctgttga gttagatgac
    20941 caaattgttt tggatgctta tgattctggg agtatgatta cttatgagga agtagatgtt
    21001 gatgaagatg agttgacagg tcgtagacgt tcaagtaaga aaaagaagaa aaaaacatca
    21061 tttttacctt tattttattt tatccttttt gctttatcga ttttaatttt tgtgacttat
    21121 tatgtttgga actatattca aactcaacca gaggagcctt ctctttctaa ttacagtgtg
    21181 gttcaatcaa caagttcaac tagctctgtt ccccactcct caagtagtag ttcttctagt
    21241 atagaatcag ctataagtgt atcaggcgaa ggaaatcatg tagaaatcgc ttataagaca
    21301 agtaaggaaa cagttaaatt gcaattggca gtttcagatg ttacaagttg ggtcagtgtt
    21361 tcagaaagcg aacttgaggg cggtgtaacc ttatcgccaa agaagaaaag tgcagaagca
    21421 acagttgcaa ctaaaagtcc tgtaacaatt acgttaggtg ttgtaaaagg tgttgatttg
    21481 acagtagata atcagactgt tgatttatcg aaattaacag ctcagactgg acaaatcact
    21541 gtaaccttta ctaaaaatta aggaaaaacg aatgaaaaaa gaacaaattc ccaatctctt
    21601 aacaataggt cgaattctct ttatacctat ttttatcttt attttaacga taggaaattc
    21661 gatagagagt catatagttg cagctattat ctttgctgtt gccagtatta ccgactattt
    21721 agatggatat ttagctcgta aatggaatgt ggtcagtaat tttggtaaat ttgcagatcc
    21781 tatggcggat aagttactag ttatgtcggc ttttattatg ttgattgagt taggtatggc
    21841 tccggcttgg attgttgcag tgattatctg tcgtgagtta gctgtgacag gtttaaggct
    21901 tttattggtt gaaactggtg gaacaatttt agcagcagca atgcctggaa aaattaaaac
    21961 ttttagtcag atgtttgcta ttattttctt gctattacat tggactttgc ttggtcaagt
    22021 tctactttat gtagccttat ttttcactat ctactctggc tatgactatt tcaagggtag
    22081 tgcctatgta tttaaaggga catttggttc gaaatgaaat caataattga tgtaaaaaat
    22141 ctttcttttc gctataaaga aaatcagaac tactacgatg tgaaggatat tacgtttcac
    22201 gtgaaacgtg gagaatggct ttcgattgta gggcataatg gtagtggtaa atcaacgacg
    22261 gttcgattaa ttgatggctt actggaagca gaatccggag agattgtaat tgatggccaa
    22321 cgtttgactg aggaaaatgt ttggaatata cgtcgtcaaa tcggtatggt ttttcaaaat
    22381 ccagacaatc aatttgttgg agcgactgtt gaagatgatg ttgcttttgg tttggaaaat
    22441 cagggacttt ctcgtcaaga aatgaaaaag agagtggaag aagctctggc tttagttggc
    22501 atgttggact ttaaaaagag agagccagcg cgtctatcag gtggccaaaa gcaacgtgtg
    22561 gccattgcag gtgttgtagc cctaagacca gctattttaa tcttagatga agcaacgagt
    22621 atgttggatc ctgaggggcg tagagaactt attgggacag taaaaggaat tcgaaaagac
    22681 tatgatatga cggtcatttc tattacccat gatttggaag aagtcgccat gagtgatcgc
    22741 gtattggtca tgaaaaaagg ggaaattgaa tcaactagta gtccaaggga gcttttctct
    22801 cgaaatgatt tagatcaaat tggattagac gatccttttg ctaatcaatt aaaaaaatct
    22861 ttgagccaga atggctatga tttacctgaa aattatttga cagaaagtga gctagaggat
    22921 aagctatggg aattgctcta gaaaatgtga attttacata tcaagaaggt actcccttag
    22981 cttcagcagc tttgtcggat gtttctttga cgattgaaga tggctcttat acagctttaa
    23041 ttgggcacac aggtagtggt aaatcaacta ttttacaact cttaaatggt ttattggtgc
    23101 caagtcaagg gagtgtgagg gtttttgata ccttaatcac ctcgacttct aaaaataaag
    23161 atattcgtca aattagaaaa caggttggct tggtatttca gtttgctgaa aatcagattt
    23221 ttgaagaaac ggttttgaag gacgttgctt ttggaccgca aaattttgga gtttctgaag
    23281 aagatgctgt gaagactgcg cgtgagaaac tggctctggt tggaattgat gaatcacttt
    23341 ttgatcgtag tccgtttgag ctgtcagggg gacaaatgag acgtgttgcc attgcaggca
    23401 tacttgccat ggagccagct atattagtct tagatgagcc aacagctggt ctagatcctc
    23461 tagggagaaa agagttgatg accctgttca aaaaactcca ccagtcaggg atgaccatcg
    23521 tcttggtaac gcatttgatg gatgatgttg ctgaatatgc gaatcaagtc tatgtaatgg
    23581 aaaagggacg tttagtaaag gggggcaaac caagtgatgt ctttcaagac gttgttttta
    23641 tggaagaagt tcagttggga gtacctaaaa ttacggcctt ttgtaaacga ttggctgata
    23701 gaggcgtgtc atttaaacga ttaccgatta agatagagga gttcaaggag tcgctaaatg
    23761 gatagtatga ttttagggcg ttatatccca ggggattcga ttgttcaccg attggatcca
    23821 cgtagcaaat tgcttgctat gatgctactg attttgattg tgttttgggc taataacccc
    23881 ttgacgaatc tgattctttt tatagcgaca gggatattta ttgccttgtc aggagtatct
    23941 ctttcatttt ttattcaggg cttgaagtct atgtttttct tgattgcctt cacaactatt
    24001 tttcaactat ttttcatttc taatgggaat gttttatttg agttttcgtt tgtgagaatc
    24061 acggattatg ctttgcaaca agctgggatt attttttgtc gctttgtatt gattattttc
    24121 ttttcaactt tgttaacctt aacaaccatg cctttaagtt tggcatcagc tgtcgaagct
    24181 ttattagcac ctttaaagcg tgtgaaagtt ccagttcatg aaattggatt gatgctgtct
    24241 atgagtttgc gttttgtccc aaccttgatg gatgatacga ctcggattat gaatgcacag
    24301 aaagctcgtg gagtggattt tggagaagga agcatcgttc aaaaagtaaa ggcgatgatt
    24361 cccattttga ttcctctttt tgcgacaagt ttaaaacgtg cagattcctt ggctatcgct
    24421 atggaagcgc gtggctatca gggtggaaaa ggcagaagtc aataccgaca attgaaatgg
    24481 actctaaagg atacgctgac cattcttgtt attctcgtac ttggttgttg tttatttttc
    24541 ttaaaatctt agtagctttc aggaattgat aaaagttact gtaacagttt ttgatataaa
    24601 ggtagggata tgaaccgttt taaaaaatca aaatatgtca ttattgtttt tgtcactgtt
    24661 ctgcttgtct cagctctctt agcgacgact tattcaagta caattgtgac aaaattagga
    24721 gatgggatct cattggttga tagagttgtg caaaaacctt ttcagtggtt tgattctgtt
    24781 aaatcagatt tggcacattt gacacgagca tataatgaaa atgaaagttt gaagaaacag
    24841 atttaccaat tagaagttaa atcaaatgag gcggaaagtt taaagacaga aaatgaacaa
    24901 ttgcgtcaat tgcttggtat gaagtccaag ttgcaagcta caaagacttt ggcagcagat
    24961 gttattatgc gttctccggt atcttggaag caggaattga ccttagatgt aggtaaatca
    25021 aaaggggctt ctgagaacat gttagctatt gcaaatggtg gcttgattgg gagtgtttca
    25081 aaagtagagg agaactctac tatagtcaac cttctgacta atacggaaaa tgctgataag
    25141 atttctgtta aaattcaaca cggcactacc acaatttatg gaattattgt tggctacgac
    25201 aaggaaaatg aggttcttaa aattagtcaa ttaaatagta atagcgatat tagtacaggc
    25261 gataaggtga caacgggtgg attaggaaac tttaacgttg cggatattcc tgttggtgaa
    25321 gtagttgcca caacgcatag tacagactat ttgacacgag aagtaactgt taaattgagt
    25381 gcagatactc ataatgtgga tgtgatagaa ttagtgggga attcataatg agacagttga
    25441 agcgagttgg agtattttta ttgcttcctt tctttgttct aattgatgcc catattagcc
    25501 agcttctggg ctcatttttc ccccatgtac atttggctag tcattttctt tttctatttc
    25561 tcttatttga gacgatagaa gtgtcagagt atctctacct agtctattgt tttgtgatag
    25621 gcttggttta cgatgtttac tttttccatc taatagggat tgcaactctc ttatttatct
    25681 tattgggagc ctttcttcat aaattgaata gtgttatttt attgaatcgt tggacgagaa
    25741 tcttggctat gattgttatg agttttctat ttgatatggg aacttatctc tttgcattag
    25801 tggtaggttt aactctagat agtctgccga tttttatcgt ttatagccta gtaccatcca
    25861 tgattttaaa tcttgtgtgg atgcttattt ttcagtttat ttttgaaaga tattatctat
    25921 aagaaagaaa caaaaatgta acaaaggcgt aatattcatt aggccttttt ttggtatact
    25981 agtattgtct ttaaaagaag gagtatctac gtaatatgaa gaaaaaaatc ttagcgtcac
    26041 ttttattaag tacagtaatg gtttctcaag tagctgtttt aacaactgcg catgcagaaa
    26101 ctactgatga caaaattgct gctcaagata ataaaattag taacttaaca gcacaacaac
    26161 aagaagccca aaaacaagtt gaccaaattc aggagcaagt atcagctatt caagctgagc
    26221 aatctaactt gcaagctgaa aatgatagac tacaagcaga atctaaaaaa cttgagaatg
    26281 agattacaga actttctaaa aacattgtat ctcgcaatga ttctttggaa aaacaggctc
    26341 gtagtgctca aacaaatgga gctgcaacta gctacatcaa tacgattgta aactcaaaat
    26401 caattacaga agctatttca cgtgtagcag caatgaatga aatcgtatct gctaacaaca
    26461 aaatgttgga acaacaaaaa gcagataaaa aagcaatttc tgaaaaacaa gtagcaaaca
    26521 atgatgcaat taatacagta attgcaaatc aacagaaatt gtctgatgat gcgcaagctt
    26581 taacaacgaa acaagctgag ttgaaggctg cagaattaaa tcttgctgct gaaaaagcga
    26641 cagcagaaga tgaaaaagca agtttgctag agaaaaaagc agaagcagaa gtagcagcaa
    26701 aagtagcagc agaggcagaa gcagcttaca aggcaaaaca agtaagtcaa caacaaacag
    26761 ttgttgcttc tgggaatacg acttttgcag cacaggttca agctgtagct tcttcggaat
    26821 cagcaacata tactcctgtg gcagtcaaac aacgcccaac atacagtaca aatgcttcaa
    26881 gctatccaat tggagaatgt acatggggag ttaaaacatt agcaccttgg gctggagact
    26941 attggggtaa tggagcgcag tgggctacaa gtgcagctgc tgcaggattc cgtacaggtt
    27001 caacacctca agttggtgcg attgcatgtt ggaatgatgg tggctatgga cacgtagcgg
    27061 tagtcactgc tgtagaatca acaacacgta tccaagtttc agaatcaaat tatgctggta
    27121 atcgcacgct tggaaatcat cgtgggtggt tcaatccgac aactacgtct tcaggttttg
    27181 taacatatat ttatgcagat taatttacag agggactcga atagagccct cttttcaggt
    27241 tttactgtga taatctctat taaaaattat atcaaaatag cttgaaaata ttgaaaaagt
    27301 atggtaaaat gaaaattgtc gtgtgaacga taatactcat tcttgatgaa ttgtgaagca
    27361 gttgcccttg ggtcgttttg cgagttgaag tcaagaagag gaaaaaaaac aaaaaggaga
    27421 aatactcatg gcagtaattt caatgaaaca acttcttgag gctggtgtac actttggtca
    27481 ccaaactcgt cgctggaacc ctaagatggc taaatacatc tttacagagc gtaacggaat
    27541 ccacgttatc gacttgcaac aaactgtaaa atacgctgac caagcatacg acttcatgcg
    27601 tgatgcagca gctaacgatg cagttgtatt gttcgttggt actaagaaac aagcagctga
    27661 tgcagttgct gaagaagcag tacgttcagg tcaatacttt attaaccacc gttggttggg
    27721 tggaactctt acaaactggg gaacaatcca aaaacgtatc gctcgtttga aagaaatcaa
    27781 acgtatggaa gaagatggaa ctttcgaagt tcttcctaag aaagaagttg cacttcttaa
    27841 taaacaacgt gcacgtcttg aaaaattctt gggtggtatc gaagatatgc ctcgtatccc
    27901 agatgttatg tacgtagttg acccacataa agagcaaatc gctgttaaag aagctaagaa
    27961 attgggaatc cctgttgtag cgatggttga cacaaacact gatccagatg atatcgatgt
    28021 aatcatccca gctaacgatg atgctatccg cgctgttaaa ttgatcacag ctaaattggc
    28081 tgacgctatc atcgaaggac gtcaaggtga agatgcagca gcagttgaag cagaatttgc
    28141 agcttcagaa gctcaagcag attcaatcga agaaatcgtt gaagttgtag aaggcgacaa
    28201 cgcttaattc atataaatag taattaccta ggagggcggg gcttagcccg gctctcctat
    28261 ttttaaaaaa tataggagaa tcaaaatggc agaaattaca gctaaacttg taaaagagtt
    28321 gcgtgaaaaa tctggtgccg gagttatgga cgctaaaaaa gcgcttgtag aaacagacgg
    28381 tgacatcgaa aaagcgattg aattgcttcg tgaaaaaggt atggctaagg cagctaagaa
    28441 ggctgaccgt gttgctgcag aaggattgac tggtgtttat gttaacggta atgttgcagc
    28501 agttattgaa gtaaatgctg aaactgactt cgttgcgaaa aacgctcaat tcgttgaatt
    28561 ggtaaatact acagctaaag ttattgctga aggaaaacct gctaacaatg aagaagctct
    28621 tgctttgaca atgccttcag gtgaaactct tgaagctgca tacgtatctg caacagcaac
    28681 tatcggagaa aaaatctcat tccgtcgctt tgctttgatt gaaaaaacag atgcacaaca
    28741 ctttggagca taccaacata acggtgggcg tatcggtgtt atctctgtaa tcgaaggtgg
    28801 agacgaagca cttgctaaac aaatttcaat gcacatcgct gctatgaaac caacagttct
    28861 ttcatacaaa gaattggatg agcaatttgt taaagatgag ttggcacaat tgaaccacgt
    28921 aatcgaccaa gacaacgaaa gccgtgcaat ggttaacaaa ccagctctac cacacttgaa
    28981 gtatggatca aaatctcaat tgactgatga agtgattgct caagctgaag ctgacatcaa
    29041 agctgagttg gctgcagaag gcaaaccaga aaaaatctgg gacaaaatca tcccaggtaa
    29101 aatggatcgc ttcatgcttg acaacactaa agttgaccaa gcttacacac ttcttgcaca
    29161 agtttacatc atggatgata gcaagacagt tgaagcatat cttgaatcag ttaacgcttc
    29221 agtagttgag ttcgctcgct ttgaagttgg tgaaggtatc gaaaaagctg caaacgactt
    29281 tgaagctgaa gttgcagcta caatggcagc agccttgaat aactaattat agaaaggaag
    29341 taaatttgct tccttttttc tatgcagtcg ctccttatag ggagaacttc ctattttcaa
    29401 ggtaaaaata aaaagcccta taataagggc caattgtatt taggagacta aacttcaaat
    29461 tcatagagtg ctgtagagag ataacgttca ccattatctg gtgctagagc aaggactttt
    29521 ttacctgtac ctaatttttt ggcaatctcg atggctccgt agatagctgc agctgaagaa
    29581 atccctacaa ggaaaccttc ttttccacca atttcacggc cgagtgctag agcatcgtct
    29641 gacgttacac gaacgatacc atcataagcc tttgtatcca gtgtatcagg aataaatcca
    29701 gctgagatac cttgaatttt gtgaggacca ggtttttcac cagatagaat agcagattca
    29761 tcagcttcca ctgcataaac ttgaatgttt gaatttgctg ttttgagtgc atgagaaaca
    29821 ccagaaatcg ttccaccggt acctactcca gcaacaaagg catctaatcc atctttaccg
    29881 aaagcagcta gtatctcagc tcctgttgtt ctttcgtgta cttctggatt agctggattg
    29941 tcaaattgaa gaggaaggaa accatcacgt tcagcagcga tttcttgagc cttagcaata
    30001 gcacctttca ttccctcgct accaggagtt aggacgagtt cagcaccata agcttggata
    30061 attttacgtc gttctacact catagtttca ggcataacga tgacgacttt atacccttta
    30121 gcagcaccta cccatgaaag tccaatacca gtgtttccac ttgttgcttc aacaatagta
    30181 gaaccaggtt tcagaatacc atcttgttca gctttttcaa tcatgctaag ggcaatacgg
    30241 tcttttacag atgaaccagg attaaatgct tcaagcttta tatagacgtc tgcagcacct
    30301 tctggcacga tgttgttaag tttaacaatc ggtgtttgac cgattaattc agtaatgttg
    30361 ttataaatag acatatgaat acctctttgt ataagatatc tttagtataa cgctatctat
    30421 accatttgta aaatatagaa atcctatcga agcgatagga tttctatata tcatagatca
    30481 aagtcattaa ttttcaagtg attgcgttca gctaattcta gtaaataggt gtgaaggaaa
    30541 acgatatcag gtttcgtttg aattgcttca ttttctagta tattttggta cgcatctttt
    30601 tattttgctc gctaaagtag ttgtgagaat gtcaacgaaa cgctgtatta ttcattgttt
    30661 acactaagtt tacaggaact tcaacctctc gtaaaccttg gtcagttaaa gtgacttttc
    30721 cattaaaaaa ctccacaagt gcagctttaa tagtttcttt ttcttcctta tcaacataaa
    30781 tcatcgtatc gacttgatct gtaaagtttg tatccagctc catgagatca tgttctttaa
    30841 ggaagttact gtactcttgg tactgagcat aagacatttg aatagcaatg ccagcctgtt
    30901 cttttatttc aataatacca atttctttga cagctaaggc gacactgccg gcgtaagcac
    30961 gaattagtcc tccagcgcct agtttaatac caccaaagta gcgtgtcacg accacacaga
    31021 cattggtgag attgtgattt tctagtaccc caagcatggg aacaccagca gtaccactag
    31081 gctcaccatc atcacttgta cgtttaattt cactacgttc tccaataata aaggcagagc
    31141 agttatgtgt cgctttgtag tgttcttttt tgatggcagt aatgaagtca cgagcctctt
    31201 cttcgctata aacacgcttg gcatggcaga taaagcgaga ttttttgatt tcttcttgga
    31261 cttggccgtc ctctttaatt gtcctaaatt ccatgattta attgtactaa aaaatcatct
    31321 aaagtgctag tttttacgaa taaagaagta tgaaagtaaa tttagattat ctcggttgtt
    31381 tatttactga gaatgaatta acagaagaag aacgtcagtt ggcggagaaa cttccagcaa
    31441 tgagaaagga gaaggggaaa cttttctgtc aacgttgtaa tagtactatt ctagaagaat
    31501 ggtatttgcc catcggtgct tactattgtc gagagtgctt gctgatgaag cgagtcagaa
    31561 gtgatcaaac tttatactat tttccgcagg aggattttcc gaagcaagat gttctcaaat
    31621 ggcgcggcca attaactcct tttcaagaga aggtgtcaga gggactgctt caagcagtag
    31681 acaagcaaaa gccaacctta gttcatgcgg taacaggagc tggaaagaca gaaatgattt
    31741 atcaagtagt ggctaaagtg atcaatgcgg gtggtgcagt gtgtttggct agtcctcgca
    31801 tagatgtttg tttggagctg tacaagcgcc tgcaacagga tttttcttgc gggatagctt
    31861 tgctacatgg agaatcggaa ccttattttc gaacaccact agttgttgca acaacccatc
    31921 agttattgaa gttttatcaa gcttttgatt tgctgatagt ggatgaagta gatgcttttc
    31981 cttatgttga taatcccacg ctttaccatg ctgtcaagaa tagtgtaaag gagaatggat
    32041 tgagaatctt tttaacagcg acttcgacca atgagttaga taaaaaggtc cgtttaggag
    32101 aactaaaaag actgaattta ccgagacggt ttcatggaaa tccgttgatt attccaaaac
    32161 caatttggtt atcggatttt aatcgctact tagacaagaa tcgtttgtca ccaaagttaa
    32221 agtcctatat tgagaagcag agaaagacag cttatccgtt actcattttt gcttcagaaa
    32281 ttaagaaagg ggagcagtta gaagaaatct tacaggagca atttccaaat gagaaaattg
    32341 gctttgtatc ttctgtaaca gaggatcgat tagagcaagt acaagctttt cgagatggag
    32401 aactgacaat acttatcagt acgacaatct tggagcgtgg agttaccttc ccttgtgtgg
    32461 atgttttcgt agtagaggcc aatcatcgtt tgtttaccaa gtctagtttg attcagattg
    32521 gtggacgagt tggacgaagc atggatagac cgacaggaga tttgcttttc ttccatgatg
    32581 ggttaaatgc ttcaatcaag aaggcgatta aggaaattca gatgatgaat aaggaggctg
    32641 gtctatgaag tgcttgttat gtgggcagac tatgaagact gttttaactt ttagtagtct
    32701 cttacttctg aggaatgatg actcttgtct ttgttcagac tgtgattcta cttttgaaag
    32761 aattggggaa gagaactgtc caaattgtat gaaaacagag ttgtcaacaa agtgtcaaga
    32821 ttgtcaactt tggtgtaaag aaggagttga agtcagtcat agagcgattt ttacttacaa
    32881 tcaagctatg aaggattttt tcagtcggta taagtttgat ggagacttcc tgttaagaaa
    32941 agttttcgct tcatttttaa gtgaggagtt gaaaaagtac aaagagtatc aatttgttgt
    33001 aattccccta agtcctgata gatatgctaa tagaggattt aatcaggttg agggcttggt
    33061 agaggcagca ggctttgagt atctggattt attagagaaa agagaagaga gagccagttc
    33121 ttctaaaaat cgttcagagc gcttggggac agaacttcct ttctttatta aaagtggagt
    33181 cactattcct aaaaaaatcc tacttataga tgatatctat actacaggag caactataaa
    33241 tcgtgttaag aaactgttgg aagaagctgg tgctaaggat gtaaaaacat tttcccttgt
    33301 aagatgagga aaaaatttgc aaaaatagaa tgaaagcgtt ataataaaat cataaaaaca
    33361 aaaatgttta gaaagaaggt actcatatga ttaaatatag tatccgtggt gaaaacctag
    33421 aagtaacaga agcaattcgt gattatgtag tttctaaact cgaaaagatc gaaaagtact
    33481 tccaaccaga acaagagttg gatgcccgaa ttaacttaaa agtttatcgt gaaaaaacgg
    33541 ctaaagtgga agtaacgatt ccgcttggat ctattactct ccgcgcagaa gatgtatctc
    33601 aagatatgta tggttcaatt gaccttgtaa ctgataaaat tgaacgtcag attcgtaaaa
    33661 ataaaacaaa aatcgagcgt aaaaataaaa ataaggtagc aactggtcaa ttatttacag
    33721 atgctttggt ggaagattca aatattgtcc agtctaaagt tgttcgttca aaacaaattg
    33781 atttaaaacc aatggatttg gaagaagcaa ttctacaaat ggatttattg gggcatgatt
    33841 tctttatcta tgtggatgtt gaagatcaga caaccaatgt gatttatcgt cgtgaggatg
    33901 gcgaaattgg tttgttagag gttaaagaat cttaatttca atatgtgtaa aggtaggttt
    33961 actgaattgt aaactacctt tttcttataa ttgatagaat tactgattac acttttaaaa
    34021 agtcgctttt tgaggggtat tatggtataa tgggtgccaa gaggttttga atgaaaaaat
    34081 tttatgtaag tccaattttt cctattctag taggattgat tgcgtttgga gtcttatcca
    34141 ctttcattat ttttgttaat aataatctgt tgacggtttt aattttgttt ctttttgtag
    34201 gaggctatgt ttttttattt aagaaactga gagtgcatta tacaaggagt gatgtagaac
    34261 agatacagta tgtaaaccac caagcggaag aaagtttgac agctctattg gaacagatgc
    34321 ctgtaggtgt tatgaaattg aatttatctt ctggagaggt tgagtggttt aatccctatg
    34381 ctgaattgat tttgaccaag gaagatggtg attttgattt agaagctgtt caaacgatta
    34441 tcaaggcttc agtaggaaat ccgtctactt atgccaagct tggtgagaag cgttatgctg
    34501 ttcatatgga tgcttcttcc ggtgttttgt attttgtaga tgtatccagg gaacaagcca
    34561 taacagatga attggtaaca agtagaccag tgattgggat tgtctctgtg gataattatg
    34621 atgatttgga ggatgcaact tctgagtcag atattagtca aatcaatagt tttgtagcta
    34681 attttatatc agagttttca gaaaaataca tgatgttttc tcgtcgggta agtatggatc
    34741 gattttatct atttactgac tacacggtgc ttgagggctt gatgaatgat aaattttctg
    34801 ttattgatgc tttcagagaa gagtcgaaac agagacagtt gcccttgacc ttaagtatgg
    34861 gattttctta tggcgatgga aatcatgatg agatagggaa agttgctttg ctcaatttga
    34921 acttggctga agtacgtggt ggcgaccagg tggttgttaa ggaaaacgac gaaacgaaaa
    34981 atccagttta ttttggtggt gggtctgctg cttcaatcaa gcgtacacgg actcgtacgc
    35041 gcgctatgat gacagctatt tcagataaga ttcggagtgt agatcaggtt tttgtagtcg
    35101 gtcacaaaaa tttagacatg gatgctttgg gctctgctgt aggtatgcag ttgttcgcca
    35161 gcaatgtgat tgaaaatagc tatgctcttt atgatgaaga acaaatgtct ccagatattg
    35221 aacgagctgt ttcattcata gaaaaagaag gagttacgaa gttgttgtct gttaaggatg
    35281 caatggggat ggtgaccaat cgttctttgt tgattcttgt agaccattca aagacagcct
    35341 taacattatc aaaagaattt tatgatttat ttacccaaac cattgttatt gaccaccata
    35401 gaagggatca ggattttcca gataatgcag tcattactta tatcgaaagt ggtgcaagta
    35461 gtgccagtga gttggtaacg gaattgattc agttccagaa ttctaagaaa aatcgtttga
    35521 gtcgtatgca agcaagtgtc ttgatggctg gtatgatgtt ggatactaaa aatttcacct
    35581 cgcgagtaac tagtcggaca tttgatgttg ctagctatct cagaacgcgc ggaagtgata
    35641 gtattgctat ccaggaaatc gctgcgacag attttgaaga atatcgtgag gtcaatgaac
    35701 ttattttaca ggggtgtaaa ttaggttcag atgtactaat agcagaggct aaggacacga
    35761 aatgctatga tacagttgtt attagtaagg cagcagatgc catgttagcc atgtcaggta
    35821 ttgaagcgag ttttgttctt gcgaagaata cacaaggatt tatctctatc tcagctcgaa
    35881 gtcgtagtaa actgaatgta caacggatta tggaagagtt aggcggtgga ggccacttta
    35941 atttggcagc agctcaaatt aaagatgtaa ccttgtcaga agcaggtgaa aaactgacag
    36001 aaattgtatt aaatgaaata aaggaaaagg agaaagaaga atgaaagtaa tctttttagc
    36061 agatgttaaa ggaaaaggta aaaaaggcga aattaaggaa gtaccaacag ggtatgcgca
    36121 aaactttctt atcaaaaaga atctagccaa agaagcgact gctcaagctg taggtgaact
    36181 tcgtggtaaa caaaaatcgg aagaaaaagc tcacgctgag atgattgcag aaggaaaagc
    36241 aattaaagca caacttgaag cagaagaaac tgttgtagaa tttgttgaaa aagttggtcc
    36301 agatggtcgt acctttggtt ctattaccaa taagaagatt gcagaagaat tgcaaaagca
    36361 atttggaatt aagattgata aacgtcatat tcaagtacaa gctccgattc gagcggttgg
    36421 tttgattgat gtgccagtga aaatctatca agatatcaca agtgtaatca atcttcgtgt
    36481 gaaagaagga taagtttaca ccttcttgac aagattgtaa aaggaaggga agtctgatgg
    36541 cagaagtaga agagttacga gtacaacctc aagatatctt agctgagcaa tccgttttag
    36601 gggctatctt tattgatgag agtaaacttg tttttgtgcg agaatacatt gagtctcggg
    36661 acttttttaa gtatgcccat cgtttgattt tccaagccat ggtcgattta tccgatcatg
    36721 gtgatgccat agatgcaaca acggttcgta ctatccttga taatcaaggt gatttacaga
    36781 atattggtgg cttgtcttac ttggttgaga ttgttaattc tgtgccaact tctgctaatg
    36841 cggagtatta tgctaagatt gttgcagaaa aagcaatgct acgtcgttta attgccaagt
    36901 tgacagagtc tgtcaaccaa gcttacgaag cgtcacaacc agctgatgaa attattgctc
    36961 aggcagaaaa agggttgatt gatgtcagtg aaaatgcaaa tcgaagcggg tttaagaaca
    37021 ttcgagatgt gttgaatctc aactttggaa atctggaagc tcgctcgcaa caaacgaccg
    37081 atattacagg tattgcgaca ggttatcgtg atttggatca tatgacaaca ggacttcatg
    37141 aggaggagtt gattatctta gcagctcgtc cagcagttgg taagacagca tttgccttga
    37201 atatcgctca gaacattggg actaagttgg acaaaacggt tgctattttt tcactcgaaa
    37261 tgggtgcgga aagcttggta gatcgtatgt tagctgcaga aggcttagtg gagtcacatt
    37321 ctatccgtac agggcaattg acagatgagg agtggcaaaa atatactatt gctcagggta
    37381 atctagctaa cgccagtatc tatatcgatg atacgccagg tattcggatt acagagattc
    37441 gttctcgttc tcgtaaattg gctcaagaaa ctggaaatct tggtttgatt ttgatagact
    37501 atttgcaact tatcacggga actggtcgag aaaatcgtca acaagaagtt tctgaaattt
    37561 ctcgtcagtt gaaaatacta gccaaggaat tgaaggttcc agtaatcgct ctgagtcagc
    37621 tttctcgtgg tgtagaacaa cgtcaggaca agagaccggt cttgtctgat attcgtgaat
    37681 ctgggtctat tgagcaggac gctgatatcg tagcttttct ctatcgcgat gactactatg
    37741 aacgtggtgg tgaagaagag gagggtatcc caaataataa ggtggaagtt attatcgaga
    37801 aaaaccgtag tggagctcgt ggaacagtgg aattgatttt ccaaaaagaa tacaataaat
    37861 tttcaagtat ctcaaagagg gaggcataag atgtcagatg catttacaga tgtagccaag
    37921 atgaaaaaaa tcaaagaaga aatcaaggca catgagggac aagtcgtaga aatgactttg
    37981 gagaatggtc gtaagcgcca aaaaaataga ttgggtaagc taattgaagt ttatccatcc
    38041 ctatttattg tggagtttgg ggatgtggaa ggagataaac aagttaatgt ttacgttgaa
    38101 tcctttactt actcagatat tcttacagaa aagaatttga ttcattatct tgactaaagt
    38161 gagaaatttt ctcacttttt cttttttctc cgaataattt aggtgaaggc aatcatcgct
    38221 ttatattatt tttcaaggag gaagaatgaa aattttaccg tttatagtaa gaggaacaag
    38281 ttattacttg aagatgtcag ttaaaaagct tgttcctttt ttagtagtag gattgatgct
    38341 agcagctggt gatagtgtct atgcctattc cagaggaaat ggatcgattg cgcgtgggga
    38401 tgattatcct gcttattata aaaatgggag ccaggagatt gatcagtggc gcatgtattc
    38461 tcgtcagtgt acttcttttg tagcctttcg tttgagtaat gtcaatggtt ttgaaattcc
    38521 ggcagcttat ggaaatgcga atgaatgggg acatcgtgct cgtcgggaag gttatcgtgt
    38581 agataataca ccgacgattg gttccattac ttggtctact gcaggaactt atggtcatgt
    38641 tgcctgggtg tcaaatgtaa tgggagatca gattgagatt gaggaatata actatggtta
    38701 tacagaatcc tataataaac gaattataaa agcaaacacg atgacaggat ttattcattt
    38761 taaagatttg gatggtggca gtgttgggaa tagtcaatcc tcaacttcaa caggcggaac
    38821 tcattatttt aagaccaagt ctgctattaa aactgaaccc ctagttagtg caactgtgat
    38881 tgattactat tatcctggag agaaggttca ttatgatcaa attctcgaaa aagacggcta
    38941 taagtggttg agttatactg cctataatgg aagctatcgt tatgttcaat tggaggctgt
    39001 gaataaaaat cctctaggta attctgttct ttcttcaaca ggtggaactc attattttaa
    39061 gaccaagtct gctatcaaaa ctgaacccct agttagcgga actgtgattg attactatta
    39121 tcctggggag aaggttcatt atgatcagat acttgaaaaa gacggctaca agtggttgag
    39181 ttatactgcc tataatggaa gtcgtcgcta tatacagcta gagggagtga cttcttcaca
    39241 aaattatcag aatcaatcag gaaatatctc tagctatgga tccaataata gttcaactgt
    39301 cggttggaag aaaataaatg gtagttggta tcatttcaaa tcaaatggtt ctaaatcaac
    39361 aggatggctg aaagacggtt ctagctggta ttatttgaaa ttatctggtg aaatgcagac
    39421 aggatggtta aaggagaatg gctcgtggta ttatctgggt agttcagggg caatgaaaac
    39481 aggctggtac caggtctctg gtaagtggta ttattcttat tcttcaggcg ccttagctgt
    39541 taatacgacg gtggatggct acagagtaaa cagtgatgga gaacgagtat agaaaattgg
    39601 agtaggagaa atttcctgct cttttctgta agcagtttcc ttgacatttt tcttattttg
    39661 cgctatcata tatacatata atatatggga gtctgtgtac agtctgagag gaagtgttaa
    39721 acttcgaccg cacctgatct gggtaatgcc agcggaggga acgatactta gtctaatttt
    39781 gcaccttttc catgtatggt aaaggttttt ctttttttta aaaggaaaac gagaagagga
    39841 ggttcttatg aaagcaagca ttgccttgca agttttaccc ctagcacagg ggattgatcg
    39901 gatagctgtt attgatcagg tcattgctta tctgcaaact caagaagtga cgatggtagt
    39961 gacaccattt gaaacggtct tggaagggga gtttgatgag cttatgcgca ttctaaaaga
    40021 agcgctggaa gtggcagggc aggaggcaga caatgtcttt gccaatgtca aaataaatgt
    40081 aggagagatt ttaagtattg atgagaaact tgaaaagtat actgagacga cacattagtc
    40141 tattgggctt tctcggagta ttgtcaatct ggcagttagc aggttttctt aaacttctcc
    40201 ccaagtttat cctgccgaca cctcttgaaa ttctccagcc ctttgttcgt gacagagaat
    40261 ttctctggca ccatagctgg gcgaccttga gagtggcttt actggggctg attttgggag
    40321 ttttgattgc ctgtcttatg gctgtgctca tggatagttt gacttggctc aatgacctga
    40381 tttaccctat gatggtggtc attcagacca ttccgaccat tgccatagct cctatcctgg
    40441 tcttgtggct gggttatggg attttgccca agattgtctt gattatctta acaacaacct
    40501 ttcccatcat cgttagtatt ttggacggtt ttaggcattg tgacaaggat atgctgactt
    40561 tgtttagtct gatgcgagca aacccttggc aaatcctgtg gcattttaaa atcccggtta
    40621 gcctgcctta cttttatgca ggtctgaggg tcagtgtctc ctacgccttt atcacaactg
    40681 tggtatctga gtggttggga ggttttgaag gtcttggtgt ttatatgatt cagtctaaaa
    40741 aattgtttca gtatgatacc atgtttgcca ttattattct ggtgtcgatt atcagtcttt
    40801 tgggtatgaa gctggtcgat atcagtgaaa aatatgtgat taaatggaaa cgttcgtaga
    40861 attagaatgt ttctgaaaaa gaaaagagga aatcaaaatg aagaaaacat ggaaagtgtt
    40921 tttaacgctt gtaacagctc ttgtagctgt tgtgcttgtg gcctgtggtc aaggaactgc
    40981 ttctaaagac aacaaagagg cagaacttaa gaaggttgac tttatcctag actggacacc
    41041 aaataccaac cacacagggc tttatgttgc caaggaaaaa ggttatttca aagaagctgg
    41101 agtggatgtt gatttgaaat tgccaccaga agaaagttct tctgacttgg ttatcaacgg
    41161 aaaggcacca tttgcagtgt atttccaaga ctacatggct aagaaattgg aaaaaggagc
    41221 aggaatcact gccgttgcag ctattgttga acacaataca tcaggaatca tctctcgtaa
    41281 atctgataat gtaagcagtc caaaagactt ggttggtaag aaatatggga catggaatga
    41341 cccaactgaa cttgctatgt tgaaaacctt ggtagaatct caaggtggag actttgagaa
    41401 ggttgaaaaa gtaccaaata acgactcaaa ctcaatcaca ccgattgcca atggcgtctt
    41461 tgatactgct tggatttact acggttggga tggtatcctt gctaaatctc aaggtgtaga
    41521 tgctaacttc atgtacttga aagactatgt caaggagttt gactactatt caccagttat
    41581 catcgcaaac aacgactatc tgaaagataa caaagaagaa gctcgcaaag tcatccaagc
    41641 catcaaaaaa ggctaccaat atgccatgga acatccagaa gaagctgcag atattctcat
    41701 caagaatgca cctgaactca aggaaaaacg tgactttgtc atcgaatctc aaaaatactt
    41761 gtcaaaagaa tacgcaagcg acaaggaaaa atggggtcaa tttgacgcag ctcgctggaa
    41821 tgctttctac aaatgggata aagaaaatgg tatccttaaa gaagacttga cagacaaagg
    41881 cttcaccaac gaatttgtga aataatgaca gaaattagac tagagcacgt cagttatgcc
    41941 tatggtcagg agaggatttt agaggatatc aacctacagg tgacttcagg cgaagtggtt
    42001 tccatcctag gcccaagtgg tgttggaaag accaccctct ttaatctaat cgctgggatt
    42061 ttagaagttc agtcagggag aattgtcctt gatggtgaag aaaatcccaa ggggcgcgtg
    42121 agttatatgt tgcaaaagga tctgctcttg gagcacaaga cggtgcttgg aaatatcatt
    42181 ctgcccctct tgattcaaaa ggtggataag gcagaagcta tttcccgagc ggataaaatt
    42241 cttgcgacct tccagctgac agctgtaaga gacaagtatc ctcatgaact tagcggtggg
    42301 atgcgccagc gtgtagcctt actccggacc tacctttttg ggcacaagct ctttctctta
    42361 gatgaggcct ttagcgcctt ggatgagatg acaaagatgg aactccacgc ttggtatctt
    42421 gagattcaca aacagttgca gctaacaact ctgatcatca cgcatagtat tgaggaggcc
    42481 ctcaatctca gcgaccgcat ctatatcttg aaaaatcgcc ctgggcagat tgtttcagaa
    42541 attaaactag attggtctga agatgaggac aaggaagtcc aaaagattgc ctacaaacgt
    42601 caaatcttgg cagaattagg cttagataag tagaaaaata gggagttggt gaagattatc
    42661 ctttaccagc gccctttttc ttttaaaaat gagaaaattt cggtataata gtcaaacaag
    42721 gtcaaggttt aaagagagag gtgggtttgt tatgagattt aaaaatacat cggatcatat
    42781 tgaggcctac atcaaggcga ttttagatca atctggtatc gtggagttgc aacggagtca
    42841 gttggcagat acctttcagg ttgttcctag tcagattaac tacgtgatca agacacgctt
    42901 tacggaaagt agaggctact tggttgaaag taagcgtggt ggcggaggct acattcgtat
    42961 aggacggatt gagttttcta gtcatcatga aatgctccgg gatctgcttt actcgattgg
    43021 tgagcgagtc agtcaagaaa tttatgagga tattctccag cttttggttg agcaggaatt
    43081 gatgaccaag caggagatga atttgctaga atcagtagct ttggatcgcg ttttaggaga
    43141 agaagctcca gttgttcgag caaacatgct acgtcaggtc atacaagagg tagatagaaa
    43201 agggaagtaa gatgaactat tcaaaagcat tgaatgaatg tatcgaaagt gcctacatgg
    43261 ttgctggaca ttttggagct cgttatctag agtcgtggca cttgttgatt gccatgtcta
    43321 atcacagtta tagtgtagca ggggcaactt taaatgatta tccgtatgag atggaccgtt
    43381 tagaagaggt ggctttggaa ctgactgaaa cggactatag ccaggatgaa acctttacgg
    43441 aattgccgtt ctcccgtcgt ttgcaggttc tttttgatga agcagagtat gtagcgtcag
    43501 tggtccatgc taaggtacta gggacagagc acgtcctcta tgcgattttg catgatggca
    43561 atgccttggc gactcgtatc ttggagaggg ctggtttttc ttatgaagac aagaaagatc
    43621 aggtcaagat tgctgccctt cgtcgaaatt tagaagaacg tgcaggctgg actcgtgaag
    43681 atctcaaggc tttacgccaa cgccatcgta cagtagctga caagcaaaat tctatggcca
    43741 atatgatggg catgccgcag actcctagtg gtggtctcga ggactatacg catgatttga
    43801 cagagcaagc gcgttctggc aagttagaac cagtcatcgg tcgggacaag gaaatctcac
    43861 gtatgattca aatcttgagc cgtaagacta agaacaaccc tgtcttggtt ggggatgctg
    43921 gtgtcgggaa aacagctctg gcccttggtc ttgcccagcg tattgctagt ggtgacgtgc
    43981 ctgcggaaat ggctaagatg cgcgtgttag aacttgattt gatgaatgtc gttgcaggga
    44041 cacgcttccg tggtgacttt gaagaacgca tgaataatat catcaaggat attgaagaag
    44101 atggccaagt catcctcttt atcgatgaac tccacaccat catgggttct ggtagcggaa
    44161 ttgattcgac tctggatgcg gccaatatct tgaaaccagc cttggcgcgt ggaactttga
    44221 gaacggttgg tgccaccact caggaagaat atcaaaaaca tatcgaaaaa gatgcggctc
    44281 tttctcgtcg tttcgctaaa gtgacgattg aagaaccaag tgtggcagat agtatgacta
    44341 ttttacaagg tttgaaggcg acttatgaga aacatcaccg tgtacaaatc acagatgaag
    44401 cggttgaaac agcggttaag atggctcatc gttatttaac cagtcgtcac ttgccagact
    44461 ctgctatcga cctcttggac gaagcagcag caacagtgca aaataaggca aagtatgtaa
    44521 aagcagacga ttcagatttg agtccagctg acaaggccct gatggatggc aagtggaaac
    44581 aggcagccca gctaatcgca aaagaagagg aagtacctgt ctacaaagac ttggtgacag
    44641 agtctgatat tttgaccacc ttgagtcgct tgtcaggaat cccagttcaa aaactgactc
    44701 agactgacgc taagaaatat ctaaatcttg aagcagaact ccataaacgg gttatcggtc
    44761 aagatcaagc tgtttcaagt attagtcgtg cgattcgccg taatcagtca ggaattcgca
    44821 gtcacaagcg tccgatcggt tcctttatgt tcctaggacc cacaggtgtc ggtaagaccg
    44881 agttggccaa ggctttggca gaagttctct ttgatgacga atcagccctt atccgctttg
    44941 atatgagtga gtatatggag aaattcgcag ccagccgtct aaatggagct cctccaggct
    45001 atgtgggtta cgaagaaggt ggggagttga cagagaaggt tcgcaataaa ccctattccg
    45061 ttctcctctt tgatgaggta gagaaggctc acccagatat ctttaatgtt ctcttgcagg
    45121 ttctggatga cggtgtcttg acagatagca agggacgcaa ggtcgatttt tcaaatacca
    45181 ttatcattat gacatcgaac ctaggtgcga ctgcccttcg tgatgataag actgttggtt
    45241 ttggggctaa ggatattcgt tttgaccagg aaaatatgga aaaacgcatg tttgaagaac
    45301 tgaaaaaagc ttatagaccg gaattcatca accgtattga tgagaaggtg gtcttccata
    45361 gcctatctag tgaccatatg caggaagtgg tgaagattat ggtcaagcct ttagtggcaa
    45421 gtttggctga aaaaggcatt gacttgaaat tacaagcttc agctctgaaa ttgttagcaa
    45481 atcaaggata tgacccagag atgggagctc gcccacttcg cagaaccctg caaacagaag
    45541 tggaggacaa gttggcagaa cttcttctca agggagattt agtggcaggc agcacactta
    45601 agattggtgt caaagcaggc cagttaaaat ttgatattgc ataaaagaat aaaagtacca
    45661 gcatctgacc ataagtcaca gtggagtgaa actcaatgaa aatcaaagag caaactaggc
    45721 agctagccgc aggttgctca aaacactggt ttgaggttgc agatagagct gacgtggttt
    45781 gaagagattt ttgaagagta taagctgcaa gatgaatgat tttcttgtat tgacgttgtt
    45841 gttgacaaaa agtagcggat aaatgaaatc cattccatta tcatagatga taggctggta
    45901 ggaaattttc aaatagcata cagaaaatag atgtatggag ttctggtagt agaaagggag
    45961 agagatgaac attttagttg cagatgacga ggaaatgatt agagaaggaa ttgcagcatt
    46021 tctgacagaa gagggttatc atgtcattat ggctaaggat ggacaagagg tcttggaaaa
    46081 atttcaagat ctccctatcc atctcatggt actggattta atgatgccta ggaagagtgg
    46141 ttttgaagtg ttaaaagaaa tcaatcaaaa gcacgatatt cctgtcatcg tcttgagtgc
    46201 tctgggagat gaaactactc agtcacaggt atttgatctc tatgctgatg atcatgtgac
    46261 aaaacctttt tctttggtac tgcttgtcaa gcgtattaag gcgcttatca gacgttacta
    46321 cgtcatagag gatatttggc gatatcagga tgtaacagtg gattttacct cttacaaagc
    46381 acattataaa aatgaagaaa ttgatctcaa accaaaggaa ttactggtac taaagtgttt
    46441 gattcagcat aaaaatcaag ttttaagtag agagcagata ttggaagaaa tttcaaaaga
    46501 tgtagctgat ttaccttgtg atagggtcgt tgatgtctat attcgtactc ttcgcaaaaa
    46561 attagcttta gattgtatcg tgactgtgaa aaatgttggg tataagatta gcttatgata
    46621 aaaaatccta aattattaac caagtctttt ttaagaagtt ttgcaattct aggtggtgtt
    46681 ggtctagtca ttcatatagc tatttatttg acctttcctt tttattatat tcaactggag
    46741 ggggaaaagt ttaatgagag cgcaagagtg tttacggagt atttaaagac taagacatct
    46801 gatgaaattc caagcttact ccagtcttat tcaaagtcct tgaccatatc tgctcacctt
    46861 aaaagagata ttgtagataa gcgactccct cttgtgcatg acttggatat taaagatgga
    46921 aagctatcaa attatatcgt gatgttagat atgtctgtta gtacagcaga tggtaaacag
    46981 gtaacagtgc aatttgttca cggggtggat gtctacaaag aagcaaagaa tattttgctt
    47041 ttgtatcttc catatacatt tttggttaca attgcttttt cctttgtttt ttcttatttt
    47101 tatactaaac gcttgctcaa tcctcttttt tacatttcag aagtgactag taaaatgcaa
    47161 gatttggatg acaatattcg ttttgatgaa agtaggaaag atgaagttgg tgaagttgga
    47221 gaagttggaa aacagattaa tggtatgtat gagcacttgt tgaaggttat tcatgagttg
    47281 gaaagtcgta atgagcaaat tgtaaaattg caaaatcaaa aggtttcctt tgtccgtgga
    47341 gcatcacatg agttgaaaac ccctttagcc agtcttagaa ttatcctaga gaatatgcag
    47401 cataatattg gagattacaa agatcatcca aaatatattg caaagagtat aaataagatt
    47461 gaccagatga gccacttatt ggaagaagta ctggagtctt ctaaattcca agagtggaca
    47521 gagtgtcgtg agaccttgac tgttaagcca gttttagtag atattttatc acgttatcaa
    47581 gaattagctc attcaatagg tgttacaatt gaaaatcaat tgacagatgc taccagggtc
    47641 gtcatgagtc ttagggcatt ggataaggtt ttgacaaacc tgattagtaa tgcaattaaa
    47701 tattcagata aaaatgggcg cgtaatcata tccgagcaag atggctatct ctctatcaaa
    47761 aatacatgtg cgcctctaag tgaccaagaa ctagaacatt tatttgatat attctatcat
    47821 tctcaaatcg tgacagataa ggatgaaagt tctggtttgg gtctttacat tgtgaataat
    47881 attttagaaa gctatcaaat ggactatagt tttctccctt atgaacacgg tatggaattt
    47941 aagattagct tgtagacaga ttagtttttt attaaagttc atatagggtt aacataagtg
    48001 tgttattctt tgtatagata aaagaaagga tactaatatg gtattagcga ttattttagt
    48061 aacattcctt attcgattga tttttttaaa gcgttcgata gagaatgaga aacgaatcct
    48121 tagcaatggc gggaaagaat ttggagttga gaatacaaaa cgattaacgt tggctcatat
    48181 tgttttttat ctctcttgcc tcgttgaagc aatggtgcac aagacaatgt ttgatggtat
    48241 gagcatggtt ggtttagtct tgcttatttt ttctatgctg atgttgatgt tggtgattca
    48301 cttgttggga gatatttgga cagtgaagct tatgcttgtc aataatcaca aatatgtaga
    48361 tcatatcttg tttaggacag taaaacatcc taattacttt ttaaatattt tacctgagtt
    48421 gattggcttg accttgttga gtcatgccta tatgactttt gttttagttt ttccagttta
    48481 tgcagttatt ttgtatcgac gaatagctga agaggaaaag ctattacatg aagttataat
    48541 cccaaatgga agcataaaga gataaataca aaattcgact catatacagt tcatattgaa
    48601 gtgatatagt aaggttaaag aaaaaatata gaaggaaata aacatgtttg catcaaaaag
    48661 cgaaagaaaa gtacattatt caattcgtaa atttagtatt ggagtagcta gtgtagtagt
    48721 tgccagcttg ttcttaggag gagtagtcca tgcagaaggg gttaggagta agaataacct
    48781 cacggttaca tctagtgggc aagatatatc gaagaagtat gctgatgaag tcgagtcgca
    48841 tctacaaagt atattgaagg atgtcaataa aaatttgaaa aaagttcaac atacccaaaa
    48901 tgtcgacttc aacaaaaagt tgagcagaat taaaacgaag tatttgtatg gattaaaaga
    48961 gaagtcggaa gctgagttga cgttaaaaac aaaagaaaca aaagaagagt taaccgcagc
    49021 ttttgagcag tttaaaaaag atacattgaa atcaggaaaa aaggtagcag aagctgagaa
    49081 aaaagccaaa gctcaaaaag aagaagatcg ccgtaactac ccaaccaata cttacaaaac
    49141 aatcgaactg gaaattgcgg aagcagaagt aggggtcgcc aaggcagagc ttgagcttga
    49201 gtttgcacaa gctcaagttc aaatacctca agatactgag aaaattaatg ctgctaaatc
    49261 taaagtagaa gctgctaaaa gtaatgttaa aaaactagaa aaaattaaat cagatattga
    49321 aaaaacgtat ttgtataaat tagataactc aaccaaagaa acgccaaaat ctagagtgcg
    49381 aagaaattct ccgcaagtag gcgattcgag agaacttaag gaaacgatag acaaagcgaa
    49441 agaaactctg tctacctata tggtaactcg tttaacgaag ctggatccat ctgttttttg
    49501 gtttgcagat cttcttatgg atgctaagaa ggttgtggaa gaatacaaga caaaattaga
    49561 ggatgcttca gataaaaaat cggtagaaga cttgcgaaag gaagcagaag gaaaaataga
    49621 gtctcttatc gtgactcacc aaaatagaga aaaagaaaac caaccagcac cccaaccagg
    49681 aggacaagca ggtggttcaa tggttgtacc accggtgacg caaacacctc catcaacttc
    49741 ccaaagtcca ggacaaaagg cgaccgaagc tgaaaagaaa aagttacaag acttgattcg
    49801 tcaattccaa gaagccttga acaaactaga cgatgaaaca aagactgttc cagatggggc
    49861 taaactcaca ggagaagctg gaaaagccta taatgagact agaacttatg cgaaagaagt
    49921 tgttgacaaa agcaagaagc ttctatcaca gacagcagtg acaatggatg aattggcaat
    49981 gcaattaacc aaattgaacg atgccatgtc taaattgaaa gaagctaaag cgaaattggt
    50041 accagaggtt aaaccacagc cggaaaaccc agagccaaaa ccacaaccag agggtgagaa
    50101 accaagcgta ccagatatta atcaggagaa agaaaaagct aaacttgcta tagcaacata
    50161 catgagcaag attttagatg atataaagaa acatcatctg aagaaagaaa aacatcatca
    50221 gattgttgct cttattaagg accttgataa acttaaaaag caagcacttt ctgaaattga
    50281 taatgtaaat accaaagtag aaattgagaa tacagtccac aaggtatttg cagacatgga
    50341 tacggttgtt actaaattcc aaaaaggctt aattcagaac acaccgcagg ttccagaagc
    50401 accaaagagc ccagaggtac caaaggtttc agatacacca aaggctccgg acacaccgca
    50461 ggttccagaa gcaccaaagg ctccggacac accgcaagtt ccagaagcac caaagagccc
    50521 agaggtacca aaggttccag atacaccaaa ggctccggac acaccgcagg ttccagaagc
    50581 accaaaggct ccagacacac cgcaaattcc ggaagcacca gctccagaaa ctccggctcc
    50641 agctccagaa gctccaaaaa caggctggaa acaagaaaac ggtatgtggt acttctacaa
    50701 tactgatggt tcaatggcaa caggctggct agaatacaat ggctcatggt actatctcaa
    50761 cgctaatggt gctatggc
//
LOCUS       DAVLVZ010000007        49176 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000007 DAVLVZ010000000
VERSION     DAVLVZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.007"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            71..292
                     /locus_tag="VYN77_000365"
     CDS             71..292
                     /locus_tag="VYN77_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEV1557881.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            720..1025
                     /locus_tag="VYN77_000366"
     CDS             720..1025
                     /locus_tag="VYN77_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1557882.1"
                     /translation="MLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSAIIFGYI
                     HINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLLLFSGS"
     gene            complement(1077..1275)
                     /locus_tag="VYN77_000367"
                     /pseudo
     CDS             complement(1077..1275)
                     /locus_tag="VYN77_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1268..2176
                     /locus_tag="VYN77_000368"
     CDS             1268..2176
                     /locus_tag="VYN77_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1557883.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2173..2634
                     /locus_tag="VYN77_000369"
     CDS             2173..2634
                     /locus_tag="VYN77_000369"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1557884.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2624..3511
                     /locus_tag="VYN77_000370"
     CDS             2624..3511
                     /locus_tag="VYN77_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1557885.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3514..5397
                     /gene="cbpE"
                     /locus_tag="VYN77_000371"
                     /gene_synonym="pce"
     CDS             3514..5397
                     /gene="cbpE"
                     /locus_tag="VYN77_000371"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1557886.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIVGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5477..6607
                     /locus_tag="VYN77_000372"
     CDS             5477..6607
                     /locus_tag="VYN77_000372"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1557887.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6617..7879
                     /locus_tag="VYN77_000373"
     CDS             6617..7879
                     /locus_tag="VYN77_000373"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1557888.1"
                     /translation="MVSKQEQFEQVQVVKKSINTASEEVKNQALLTMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHEDKAASFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAIA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            7883..8680
                     /gene="proC"
                     /locus_tag="VYN77_000374"
     CDS             7883..8680
                     /gene="proC"
                     /locus_tag="VYN77_000374"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1557889.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTIMEAVHQAYKRTQELGK"
     gene            8900..9538
                     /locus_tag="VYN77_000375"
     CDS             8900..9538
                     /locus_tag="VYN77_000375"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1557890.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLELHKKVRQGYLSLLEKEGNRIVKIDASLPLEQVVETTKAVLFDGMDLAK"
     gene            9535..10425
                     /locus_tag="VYN77_000376"
     CDS             9535..10425
                     /locus_tag="VYN77_000376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1557891.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10465..10782
                     /gene="yabA"
                     /locus_tag="VYN77_000377"
     CDS             10465..10782
                     /gene="yabA"
                     /locus_tag="VYN77_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1557892.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10785..11654
                     /gene="rsmI"
                     /locus_tag="VYN77_000378"
     CDS             10785..11654
                     /gene="rsmI"
                     /locus_tag="VYN77_000378"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1557893.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFLEIQARIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            11881..12399
                     /locus_tag="VYN77_000379"
     CDS             11881..12399
                     /locus_tag="VYN77_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1557894.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            12508..13190
                     /locus_tag="VYN77_000380"
                     /pseudo
     CDS             12508..13190
                     /locus_tag="VYN77_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(13179..13984)
                     /locus_tag="VYN77_000381"
                     /pseudo
     CDS             complement(13179..13984)
                     /locus_tag="VYN77_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            13979..14233
                     /locus_tag="VYN77_000382"
     CDS             13979..14233
                     /locus_tag="VYN77_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1557895.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            14427..15761
                     /gene="trmFO"
                     /locus_tag="VYN77_000383"
     CDS             14427..15761
                     /gene="trmFO"
                     /locus_tag="VYN77_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1557896.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            15845..16582
                     /locus_tag="VYN77_000384"
     CDS             15845..16582
                     /locus_tag="VYN77_000384"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1557897.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            16591..17148
                     /gene="frr"
                     /locus_tag="VYN77_000385"
     CDS             16591..17148
                     /gene="frr"
                     /locus_tag="VYN77_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1557898.1"
                     /translation="MANIIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            17208..18062
                     /gene="cvfB"
                     /locus_tag="VYN77_000386"
     CDS             17208..18062
                     /gene="cvfB"
                     /locus_tag="VYN77_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1557899.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            18071..18286
                     /locus_tag="VYN77_000387"
     CDS             18071..18286
                     /locus_tag="VYN77_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1557900.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            18372..19340
                     /locus_tag="VYN77_000388"
     CDS             18372..19340
                     /locus_tag="VYN77_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1557901.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(19318..19497)
                     /locus_tag="VYN77_000389"
     CDS             complement(19318..19497)
                     /locus_tag="VYN77_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557902.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            19533..20033
                     /locus_tag="VYN77_000390"
     CDS             19533..20033
                     /locus_tag="VYN77_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1557903.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGFDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            20036..20295
                     /locus_tag="VYN77_000391"
                     /pseudo
     CDS             20036..20295
                     /locus_tag="VYN77_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(20662..20988)
                     /locus_tag="VYN77_000392"
     CDS             complement(20662..20988)
                     /locus_tag="VYN77_000392"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557904.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(20934..21347)
                     /locus_tag="VYN77_000393"
     CDS             complement(20934..21347)
                     /locus_tag="VYN77_000393"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV1557905.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(21348..21773)
                     /locus_tag="VYN77_000394"
     CDS             complement(21348..21773)
                     /locus_tag="VYN77_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1557906.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            21950..22519
                     /locus_tag="VYN77_000395"
     CDS             21950..22519
                     /locus_tag="VYN77_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1557907.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKDRIVTRVHYVLTKEDYFANK"
     gene            22587..23237
                     /locus_tag="VYN77_000396"
     CDS             22587..23237
                     /locus_tag="VYN77_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV1557908.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKSAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTNGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            23221..25461
                     /locus_tag="VYN77_000397"
     CDS             23221..25461
                     /locus_tag="VYN77_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1557909.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSTSYLALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFFTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFIFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNTWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPYQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            25640..25828
                     /locus_tag="VYN77_000398"
     CDS             25640..25828
                     /locus_tag="VYN77_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557910.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            25860..26336
                     /locus_tag="VYN77_000399"
     CDS             25860..26336
                     /locus_tag="VYN77_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1557911.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLA"
     gene            26451..27632
                     /locus_tag="VYN77_000400"
     CDS             26451..27632
                     /locus_tag="VYN77_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1557912.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSVS
                     LERELETRLVDRERVINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAMSS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPS
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     misc_feature    27792..27923
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            27939..28469
                     /gene="infC"
                     /locus_tag="VYN77_000401"
     CDS             27939..28469
                     /gene="infC"
                     /locus_tag="VYN77_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1557913.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            28502..28702
                     /gene="rpmI"
                     /locus_tag="VYN77_000402"
     CDS             28502..28702
                     /gene="rpmI"
                     /locus_tag="VYN77_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1557914.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            28754..29113
                     /gene="rplT"
                     /locus_tag="VYN77_000403"
     CDS             28754..29113
                     /gene="rplT"
                     /locus_tag="VYN77_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1557915.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            29171..29551
                     /locus_tag="VYN77_000404"
     CDS             29171..29551
                     /locus_tag="VYN77_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1557916.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            29801..30601
                     /locus_tag="VYN77_000405"
     CDS             29801..30601
                     /locus_tag="VYN77_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1557917.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPENETVSQRVCEDGPVFRTGTVVL"
     gene            30612..31550
                     /locus_tag="VYN77_000406"
     CDS             30612..31550
                     /locus_tag="VYN77_000406"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1557918.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            31592..33625
                     /locus_tag="VYN77_000407"
     CDS             31592..33625
                     /locus_tag="VYN77_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV1557919.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGAIGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGVKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(33686..35341)
                     /gene="pavA"
                     /locus_tag="VYN77_000408"
     CDS             complement(33686..35341)
                     /gene="pavA"
                     /locus_tag="VYN77_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1557920.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRKQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            35446..35943
                     /gene="ybeY"
                     /locus_tag="VYN77_000409"
     CDS             35446..35943
                     /gene="ybeY"
                     /locus_tag="VYN77_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1557921.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            35924..36319
                     /locus_tag="VYN77_000410"
     CDS             35924..36319
                     /locus_tag="VYN77_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1557922.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            36336..37235
                     /gene="era"
                     /locus_tag="VYN77_000411"
     CDS             36336..37235
                     /gene="era"
                     /locus_tag="VYN77_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1557923.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            37283..38107
                     /gene="mutM"
                     /locus_tag="VYN77_000412"
     CDS             37283..38107
                     /gene="mutM"
                     /locus_tag="VYN77_000412"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1557924.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            38107..38712
                     /locus_tag="VYN77_000413"
     CDS             38107..38712
                     /locus_tag="VYN77_000413"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1557925.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDTQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            38699..39898
                     /locus_tag="VYN77_000414"
     CDS             38699..39898
                     /locus_tag="VYN77_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV1557926.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            39898..40047
                     /gene="rpmG"
                     /locus_tag="VYN77_000415"
     CDS             39898..40047
                     /gene="rpmG"
                     /locus_tag="VYN77_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1557927.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            40087..40320
                     /gene="secG"
                     /locus_tag="VYN77_000416"
     CDS             40087..40320
                     /gene="secG"
                     /locus_tag="VYN77_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1557928.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            40422..42776
                     /gene="rnr"
                     /locus_tag="VYN77_000417"
     CDS             40422..42776
                     /gene="rnr"
                     /locus_tag="VYN77_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1557929.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     AGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDCLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKKYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGESYADVLSMMLLRSMQQARYSEHNYGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            42739..43206
                     /gene="smpB"
                     /locus_tag="VYN77_000418"
     CDS             42739..43206
                     /gene="smpB"
                     /locus_tag="VYN77_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1557930.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKRQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            43222..44082
                     /gene="tehB"
                     /locus_tag="VYN77_000419"
     CDS             43222..44082
                     /gene="tehB"
                     /locus_tag="VYN77_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1557931.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMETVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            44155..45108
                     /gene="coiA"
                     /locus_tag="VYN77_000420"
     CDS             44155..45108
                     /gene="coiA"
                     /locus_tag="VYN77_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1557932.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            45127..46929
                     /gene="pepF"
                     /locus_tag="VYN77_000421"
     CDS             45127..46929
                     /gene="pepF"
                     /locus_tag="VYN77_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1557933.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLKGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALVVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHLYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            46931..47644
                     /locus_tag="VYN77_000422"
     CDS             46931..47644
                     /locus_tag="VYN77_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1557934.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGSLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            47711..48652
                     /gene="prsA"
                     /locus_tag="VYN77_000423"
     CDS             47711..48652
                     /gene="prsA"
                     /locus_tag="VYN77_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1557935.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(48770..49111)
                     /locus_tag="VYN77_000424"
                     /pseudo
     CDS             complement(48770..49111)
                     /locus_tag="VYN77_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        14845 a         8916 c        11158 g        14257 t
ORIGIN      
        1 agtttttttg taagtaggta tgagtatcag attactcaac taatctgaag aataatggag
       61 gaaatatatc atgattttaa tgacaaaaaa tataaatcta acaaatgaag aattagagct
      121 gatacaaggt ggagcagatt catatggtaa aaatcctaat ggtaggtacg attgggaaat
      181 agaaccagta ttaactctgc cggttcatgg attttgtccc agaggcacct atgatttagg
      241 atatattgga ggaggcaatc atctttgcaa aggaagtgct gcgagatttt aagtaaaatt
      301 tattagtaat atgaagaaac aagtggagaa agcagagaat ttaatatgaa aaaacgggct
      361 attcaaattt tactagcatt gtccttaatt ttttacaaat caacttggtt ttggagagtt
      421 ttcaatcacc tcgcaaagcc ctatctacca gcaagtcgtg aattttttca gattctgctt
      481 ttgatggaga gcggagttct tttcttagcg gtcatctatc tactggtttt tgcaggaaag
      541 aaaatttttc atttcaagtg gcagctgagg tacttcatct accttttact gggctacatc
      601 atttcatata tgtctgactt cctcttttcg tatttcatat ccctgtcttc aaatcagatt
      661 tctttgaatg aaacggtaga acggtagaaa tgatggggag acaggagttc ccttatttct
      721 tgctcatcgt ttgcttcatc gcccctattg ctgaggaatt gatttatcga ggtgtgctta
      781 tgacaacctt tttcaaaaac tcaccttggt acggagatgt tttgctttct gctattattt
      841 tcggttatat tcatatcaat tttgctttaa ctcctcttgc ttttttcatt tatgctagtg
      901 gaggtcttat tttagctcta ttgtatcgca tgactaaaaa tctctactat ccaatactag
      961 ttcatattct cattaatatc actgccttct gggatgtgtg gttgctccta ttttcaggaa
     1021 gttagcttac taaaataatg tcggaacttt ccggcatttt cttttttcac aaatagtcaa
     1081 cgtttttctt tccgatactg aagtggtgtg tagccactta tttttttgaa ttgattttga
     1141 aaataagatt ggcgtgagaa aggcagatag tgaagatagt taagaagaat aggatgttct
     1201 tttttccttt ttggaaaact tctaaaatat ggtataatga aaagataaag aagttggggg
     1261 gtagaagatg aacattcaac aattacgcta tgttgtggct attgccaata gtggtacttt
     1321 tcgtgaagct gctgaaaaga tgtatgttag tcagccgagt ctgtctattt ctgttcgtga
     1381 tttggaaaaa gagttgggct ttaagatttt ccgtcggacc agctcaggga ctttcttgac
     1441 ccgtcgtggg atggaatttt atgaaaaagc gcaagaattg gttaaaggat ttgatatttt
     1501 tcaaaatcag tatgccaatc ctgaagaaga aaaagatgaa ttttccgttg ctagccagca
     1561 ctatgacttc ttgccaccaa ctattacggc cttttcagag cgctatcctg actataagaa
     1621 cttccgtatt tttgaatcaa ctactgttca aatattagat gaagtggcgc aagggcatag
     1681 tgagattggg attatctacc tcaacaatca aaataaaaag gggattatgc aacgggttga
     1741 aaaattaggt ctggaggtca tcgaattgat tcctttccat acccatattt atctccgtga
     1801 gggtcatcct ttagcccaga aagaggaatt agtcatggag gatttagcgg atttaccaac
     1861 ggttcgtttc actcaagaga aagacgagta cctttattat tcagagaact ttgtcgatac
     1921 cagcgctagc tcacagatgt ttaatgtgac agaccgtgcc accttgaatg gtattttgga
     1981 gcggacggac gcctatgcga caggttctgg atttttagat agtgacagtg ttaatggcat
     2041 tacagttatt cgtctcaagg ataacctaga taaccgcatg gtctatgtta aacgtgaaga
     2101 agtggagctt agtcaagctg ggactctctt cgtagaagtc atgcaagaat attttgatca
     2161 aaagaggaaa tcatgaaaaa aagagcaata gtggcagtca ttgtactgct tttaattggg
     2221 ctggatcagt tggtcaaatc ctatatcgtc cagcagattc cactgggtga agtgcgctcc
     2281 tggattccca atttcgttag cttgacctac ctgcaaaatc gaggtgcagc cttttctatc
     2341 ttacaagatc agcagctgtt attcgctgtc attactctgg ttgtcgtgat aggtgccatt
     2401 tggtatttac ataaacacat ggaggactca ttctggatgg tcttgggttt gactctaata
     2461 atcgcgggtg gtcttggaaa ctttattgac agggtcagtc agggctttgt tgtggatatg
     2521 ttccaccttg actttatcaa ctttgcaatt ttcaatgtgg cagatagcta tctgacggtt
     2581 ggagtgatta ttttattgat tgcaatgcta aaagaggaaa taaatggaaa ttaaaattga
     2641 aactggtggt ctgcgtttgg ataaggcttt gtcagatttg tcagaattat cacgtagtct
     2701 cgcgaatgaa caaattaaat caggccaggt cttggtcaat ggtcaagtca agaaagctaa
     2761 atacacagtc caagagggtg atgtcgtcac ttaccatgtg ccagaaccag aggtattaga
     2821 gtatgtggct gaggatcttc cgctagaaat agtctaccaa gatgaggatg tggctgtcgt
     2881 taacaaacct cagggaatgg ttgtgcaccc gagtgctggt cataccagtg gaaccctagt
     2941 aaatgccctc atgtatcata ttaaggactt gtcgggtatc aatggggttc tgcgtccagg
     3001 gattgttcac cgtattgata aggatacgtc aggtcttctc atgattgcta aaaacgatga
     3061 tgcgcatcta gtacttgccc aagaactcaa agataaaaag tctctccgca aatattgggc
     3121 gattgttcat ggaaatctgc ctaatgatcg tggtgtaatt gaagcgccga ttggccggag
     3181 tgaaaaagac cgtaagaaac aggctgtaac tgctaaaggg aagcctgcag tgacgcgttt
     3241 tcacgtcttg gaacgctttg gcgattatag cttagtagag ttgcaactgg agacagggcg
     3301 tactcatcaa atccgtgtcc acatggctta tatcggccat ccagtcgctg gtgatgaggt
     3361 ctatggtcct cgcaagactt tgaaaggaca tggacaattt cttcatgcca agactttagg
     3421 ttttactcat ccgagaacag gtaagacctt ggaatttaaa gcagatatcc cagagatttt
     3481 taaggaaacc ttggagagat tgagaaagta agaatgaaaa agaaattaac tagtttagca
     3541 cttgtaggcg cttttttagg tttgtcatgg tatgggaatg ttcaggctca agaaagttca
     3601 ggaaataaaa tccactttat caatgttcaa gaaggtggca gtgatgcgat tattcttgaa
     3661 agcaatggac attttgccat ggtggataca ggagaagatt atgatttccc agatggaagt
     3721 gattctcgct atccatggag agaaggaatt gaaacgtctt ataagcatgt tctaacagac
     3781 cgtgtctttc gtcgtttgaa ggaattgggt gtccaaaaac ttgattttat tttggtgacc
     3841 catacccaca gtgatcatat tggaaatgtt gatgaattac tgtctaccta tccagttgac
     3901 cgagtctatc ttaaaaaata tagtgataat cgtattacta attctgaacg tctatgggat
     3961 aatctgtacg gttatgataa ggttttacag actgcttcag aaaaaggtgt ttcagttatt
     4021 caaaacatca cacaagggga tgctcatttt cagtttgggg acatggatat tcagctctat
     4081 aattatgaaa atgaaactga ttcatcgggt gaattaaaga aaatttggga tgacaattcc
     4141 aattccttga ttagcgtggt gaaagtcaat ggcaagaaaa tttaccttgg gggcgattta
     4201 gataatgttc atggagcaga agacaagtat ggtcctctca ttggaaaagt tgatttgatg
     4261 aagtttaatc atcaccatga taccaataaa tcaaatacca aggatttcat taaaaatttg
     4321 agtccgagtt tgattgttca aacttcggat agtctacctt ggaaaaatgg tgttgatagt
     4381 gagtatgtta attggctcaa agaacgagga attgagagaa tcaacgcagc cagcaaagac
     4441 tatgatgcga cagtttttga tattcgaaaa gacggttttg tcaatatttc aacatcctac
     4501 aagcctattc caagttttca agctggttgg cataagagtt catatggaaa ctggtggtat
     4561 caagcgcctg attctacagg agaatatgct gtcggttgga atgaaattaa gggtgaatgg
     4621 tattacttta accaaacggg tatcttgtta cagaatcaat ggaaaaaatg gaacaatcgt
     4681 tggttctatt tgacagactc tggtgttgct gctaaaaatt ggaagaaaat tgttggaatc
     4741 tggtattatt ttaacaaaga aaaccagatg gaaattggtt ggattcaaga taaagagcag
     4801 tggtattatt tggatgttga tggttctatg aagacaggat ggcttcaata tatggggcaa
     4861 tggtattact ttgctccatc aggggaaatg aaaatgggct gggtaaaaga taaagaaacc
     4921 tggtactata tggattctac tggtgtcatg aagacaggtg agatagaagt tgctggtcaa
     4981 cattattatc tggaagattc aggagctatg aagcaaggct ggcataaaaa ggcaaatgat
     5041 tggtatttct acaagacaga cggttcacga gctgtgggtt ggatcaagga caaggataaa
     5101 tggtacttct tgaaagaaaa tggtcaatta cttgtgaatg gtaagacacc agaaggctat
     5161 actgtggatt caagtggtgc ctggttagtg gatgtttcga tcgagaaatc tgctacaatt
     5221 aaaactacaa gtcattcaga aataaaagaa tccaaagaag tagtgaaaaa ggatcttgaa
     5281 aataaagaaa cgagtcaaca tgaaagtgtt acaaattctt caactagtca agatttgaca
     5341 tcctcaactt cacaaagctc tgaaacgagt gtaaacaaat cggaatcaga acagtagtag
     5401 aaaagaaggt tttagggcct tctttttcct atcaactctt ttctatttcc tgttattcat
     5461 gttataatgg ataaatatga ataatcggag tgagactatg aaatacaaac ggattgtctt
     5521 taaggtgggt acttcttctc tgacaaatga ggatggaagt ttatcacgta gtaaggtaaa
     5581 ggatattacc cagcagttgg ctatgctgca cgaggctggt catgagttga ttttggtgtc
     5641 ttcaggtgcc attgcggctg gttttggagc cttaggattt aaaaagcgtc cgactaaaat
     5701 tgctgataaa caggcttcag cagcggtagg gcaggggctt ttgttggaag aatatacaac
     5761 caatcttctc ttgcgtcaaa tcgtttctgc acaaatcttg ctgacccaag atgactttgt
     5821 ggataagcgt cgttataaaa atgcccatca ggctttgtcg gttttgctca accgtggggc
     5881 aattcctatc atcaatgaga atgatagtgt cgttattgat gagctcaagg ttggggacaa
     5941 tgacactcta agtgctcaag tagcggcgat ggtccaagca gaccttttag tttttttgac
     6001 agatgtggac ggtctctata ctggaaatcc taattcagat ccaagagcca aacgcttgga
     6061 gagaatcgag accatcaatc gtgagattat tgatatggct ggtggagctg gttcgtcaaa
     6121 cggaactggg ggtatgttaa ccaaaatcaa ggctgcaact atcgcgacgg aatcaggagt
     6181 tcctgtttat atctgctcat ccttgaaatc agattccatg attgaggcgg cagaggagac
     6241 cgaggatggt tcttactttg ttgctcaaga gaaggggctt cgtacccaga aacaatggct
     6301 tgccttctat gctcagagtc aaggttctat ttgggttgat aaaggggctg cggaagctct
     6361 ctctcaacat ggaaagagtc ttctcttatc tggtatcgtt gaagcagaag gagccttttc
     6421 ttacggtgat atcgtgacag tatttgataa ggaaagtgga aaatcacttg gaaaaggacg
     6481 cgtgcaattc ggagcatctg ctttggagga tatgttgcgt tctcaaaaag ccaagggtgt
     6541 cttgatttac cgtgacgact ggatttccat tactcctgaa atccaactac tttttacaga
     6601 attttagagg taaactatgg tgagtaaaca agaacaattt gaacaggtac aggttgttaa
     6661 aaaatcgatt aacacagcta gtgaagaagt gaaaaaccaa gccttgctaa ccatggctga
     6721 tcacttagtg gctgctactg aggaaatttt agcggctaat gccctcgata tggcagcggc
     6781 taaggggaaa atctcagatg tgatgttgga tcgtctttat ttggatgcag atcgtataga
     6841 agcgatggca agaggaattc gtgaagtggt tgccttacca gatccaatag gtgaagtttt
     6901 agaaacaagt cagcttgaaa atggtttggt tatcacaaaa aaacgtgtag ctatgggtgt
     6961 catcggtatt atctatgaaa gccgtccaaa tgtgacgtct gatgcggctg ctttgactct
     7021 taagagtgga aatgcggttg ttcttcgtag tggtaaggat gcctatcaaa caacccatgc
     7081 cattgtcaca gccttgaaga agggcttgga gacgactact attcatccaa atgtgattca
     7141 actggtggag gatactagcc gtgaaagtag ttatgctatg atgaaggcta agggctatct
     7201 agaccttctc attcctcgtg gaggagctgg cttgatcaat gcagtggttg agaatgcgat
     7261 tgtacctgtt atcgagacag ggactgggat tgtccatgtc tatgtggata aggatgcaga
     7321 cgaagacaag gcgctgtcta tcatcaacaa tgctaaaacc agtcgtcctt ctgtttgtaa
     7381 tgccatggag gttctgctgg ttcatgaaga caaggcagca agcttccttc ctcgcttgga
     7441 gcaagtgttg gttgcagatc gaaaagaagc tgggttggaa ccaattcaat tccgcctaga
     7501 tagcaaagca agccagtttg tttcaggtca agctgctcaa gcacaagact ttgataccga
     7561 gtttttagac tatattctag ctgttaaggt tgtgagcagt ttagaagaag cgattgcgca
     7621 tattgaatcc cacagtaccc atcattcgga tgccattgtg acggaaaatg ctgaagctgc
     7681 agcatacttt acagatcaag tggactctgc agcggtgtat gttaatgcct caactcgttt
     7741 cacagatgga ggacaatttg gtcttggttg tgaaatgggg atttctactc agaaattgca
     7801 cgcgcgtggt cccatgggct tgaaggaatt gactagctac aagtatgtgg tcactggtga
     7861 tggacagata agggagtaag agatgaagat tggatttatc ggtttgggga atatgggtgc
     7921 tagcttggca aaatctgtct tgcagactag gacgtcagat gagattctcc ttgccaatcg
     7981 tagtcaagtc aaggtggatg ctttcatcgc taacttcggt ggtcagactt ctaacaatga
     8041 agaaatattc gcagaagcag atgtgatttt tctaggtgtt aagcctgctc agttttctga
     8101 actgctttct caataccaga ccatccttga aaaaagagaa agtcttcttt tgatttcgat
     8161 ggcagctgga ttgaccttag aaaaactagc aagtcttatc ccaagccaac accgaattat
     8221 tcgtatgatg cctaataccc ctgcttctat cgggcaagga gtgattagtt atgccttgtc
     8281 tcctaattgc agggctgagg acagtgagat cttttgtcag cttttagcca aggctggtct
     8341 cttggttgaa ttaggagaaa gcttaatcaa tgcagcgaca ggtcttgcag gttgtggacc
     8401 agcctttgtc tatctcttta ttgaggcctt ggcagatgca ggtgttcaga cgggattacc
     8461 acgagaaaca gccttgaaaa tggcagccca aactgtggta ggagctgggc aattggtcct
     8521 agaaagccag caacatcctg gagttttgaa agatcaagtt tgtagtccag gcggttcgac
     8581 tattgctggc gtagcaagcc tagaagcgca tgcttttcga ggaacgatca tggaggcagt
     8641 tcatcaagcc tataaacgaa cacaagaact aggtaaataa gaggtagttt tatctgcctc
     8701 ttttatggtg gttgatactc ttcgaaaatc aaattcaaac tgcgtcagct tcaccttgcc
     8761 gtactcaagt acagcctgcg gctagcttcc tagtttgctc tttgattttc attgagtatg
     8821 aaatgagaag acaaaaagat tgtcacaaac ccctattttt ttgatagaat agaagtagta
     8881 aaaaagaaat gagttagaca tgtcaaaagg atttttagtc tctcttgagg gaccagaggg
     8941 agcaggcaag accagtgttt tagaggctct gctaccaatt ttagaggaaa aaggagtaaa
     9001 ggtgctgacg acccgtgaac ctggcggagt cttgattggg gagaagattc gggaagtgat
     9061 tttggatcca agtcatactc agatggatgc taaaacagag ctacttctct atattgccag
     9121 tcgcagacag catttggtgg aaaaagttct tccagccctt gaagctggca agttggtcat
     9181 catggatcgt tttatcgata gttctgttgc ctatcaggga tttggtcgtg gcttagatat
     9241 tgaagccatt gactggctca atcagtttgc gacagatggc ctcaaacccg atttgacact
     9301 ctattttgac atcgaggtgg aagaagggct ggctcgcatt gctgcgaata gtgaccgcga
     9361 ggtcaatcgt ctggacttgg aaggtttgga attgcacaag aaagtccgtc aaggctacct
     9421 ttctctcctg gaaaaagaag gaaatcgcat tgtcaagatt gatgctagtc tccctttgga
     9481 gcaagttgtg gaaactacca aggctgtctt gtttgacgga atggacttgg ccaaatgaaa
     9541 caagatcaac taaaggcttg gcaaccagct cagtttgacc gttttgtccg tatcttagaa
     9601 caagaccagc tcaatcacgc ctatctcttt tcaggtttct ttggaagctt ggaaatggcg
     9661 caatttttag ctaagagcct cttttgtacg gataaagttg gcgtcttacc atgtgagaaa
     9721 tgccgaagtt gcaagctgat tgaacaggaa gagtttccag atgtcacctt gattaagcca
     9781 gtcaatcagg tcatcaagac agaacgcatt cgggaattgg tgggacagtt ttctcaagca
     9841 gggattgaaa gccagcaaca ggtctttatt atcgagcaag cggataaaat gcatcccaac
     9901 gcagccaatt ctctgctcaa ggtcatcgaa gaaccccaga gtgaagttta tattttcttc
     9961 ttgactagcg atgaggaaaa gatgttaccg acaatccgaa gtcggactca gatcttccac
    10021 tttaaaaagc aagaagaaaa acttatctta ctcttagaac aaatgggact tgttaagaaa
    10081 aaagcgactc ttttagctaa gtttagtcaa tcgcgagctg aagcagaaaa gttggctaat
    10141 caggcaagtt tttggacctt ggtcgatgaa agtgaacgcc tgctgacttg gttagtagct
    10201 aagaaaaaag aaagttatct acaggttgcc aaattagcca acttggcaga tgataaggaa
    10261 aaacaggatc aggttttacg gattcttgaa gttctctgtg ggcaggacct cttgcaggta
    10321 agagtaagag tgattctaca agatttacta gaagctagaa aaatgtggca agctaatgtc
    10381 agctttcaaa atgccatgga atatctggtc ttgaaagaaa tataaactca aaaatgaatg
    10441 ataaagaaag gaaagggctg ttttatggac aaaaaagaat tatttgacgc gctggatgat
    10501 ttttcccaac aattattggt aaccttagcc gatgtggaag ccatcaagaa aaatctcaag
    10561 agcttggtag aagaaaatac agctcttcgc ttggaaaata gtaagttgcg agaacgcttg
    10621 ggtgaggtgg aagcagacgc tcccgtcaag gccaagcatg ttcgcgaaag tgtccgtcgt
    10681 atttaccgtg atggatttca cgtatgtaat gatttttatg gacaacgtcg agagcaggac
    10741 gaagaatgta tgttctgtga tgagttgtta tacagggagt aagcatgcaa attcaaaaaa
    10801 gttttaaggg gcagtctccc tatggcaagc tgtatctagt ggcaacgccg attggcaatc
    10861 tagatgatat gacttttcgt gctatccaga ccttgaaaga agtggactgg attgctgctg
    10921 aggatacgcg caatacaggg cttttgctca agcattttga catttccacc aagcagatca
    10981 gttttcatga gcacaatgcc aaggaaaaaa ttcctgattt gattggtttc ttgaaagcag
    11041 ggcaaagtat tgctcaggtc tctgatgccg gtttgcctag catttcagac cctggtcatg
    11101 atttagttaa ggcagctatt gaggaagaaa ttgcagttgt gacagttcca ggtgcctctg
    11161 caggaatttc tgccttgatt gccagtggtt tagcgccaca gccacatatc ttttacggtt
    11221 ttttaccgag aaaatcaggt cagcagaagc aattttttgg cttgaaaaaa gattatcctg
    11281 aaacacagat tttttatgaa tcacctcatc gtgtagcaga cacgttggaa aatatgttag
    11341 aagtctacgg tgaccgctcc gttgtcttgg tcagggaatt gaccaaaatc tatgaagaat
    11401 accaacgagg tactatctct gagttattag aaagcattgc tgaaacgcca ctcaagggcg
    11461 aatgtcttct cattgttgag ggtgccagtc agggtgtgga agaaaaagac gaggaagact
    11521 tgttcttaga aatccaagct cgtatccagc aaggtgtgaa gaaaaaccaa gctatcaagg
    11581 aagtcgctaa gatttaccag tggaataaaa gtcagctcta cgctgcctac cacgactggg
    11641 aagaaaaaca ataaagggag acaggatgta ataattctgt ctgtttctgt ttaacttaat
    11701 tagtgatggt aatataaaga tgtatcactt ggtatagaag ctttggtatt aagtttttta
    11761 ttaagcccat acggaatacc gatggttgga gcagcagtta tagcgttctt agaaggtata
    11821 aatagaaaaa taaggtcatt ttaaatcaaa ggattgataa atcagaaaga aggtgatttt
    11881 ttgcgaacat acgaaaataa agaagaacta aaagctgaga tagagaaaac atttgagaaa
    11941 tatattttag aatttgataa tattccagaa aatttaaaag ataagagagc tgatgaagtt
    12001 gacagaactc cagcagaaaa ccttgcttat caggttggtt ggaccaactt ggttcttaaa
    12061 tgggaagaag atgaaagaaa ggggcttcaa gtaaaaacac catcggataa atttaaatgg
    12121 aatcaacttg gtgaattata tcagtggttc acagatacct acgctcattt atctctgcaa
    12181 gagttgaaag caaaattaaa tgaaaatatt aattctatct ctgcaatgat tgattcgttg
    12241 agtgaggaag aattatttga accgcatatg agaaagtggg ctgatgaagc gactaaaaca
    12301 gcgacttggg aagtgtataa gtttattcat gtaaatacgg ttgcaccttt tggaactttc
    12361 agaactaaaa tcagaaaatg gaagaagata gtattataaa ttatattttt aactttaaaa
    12421 aatttcataa aaatggttac caaaggcgat agaagaaaaa ctatcgtctt tttctttgca
    12481 aatttttaag aagggaggtg atcttgcatg gactttgaat atttttataa cagagaagcg
    12541 gaaagattta acttcttaaa agtaccggag atattagttg atagagaaga atttcggggc
    12601 ttatcagcag aagcaattat cctttattcc atacttctta aacagacagg aatgtcattt
    12661 aagaataact ggatagacaa ggaaggcaga gtatttatct attttactgt cgaagaaatt
    12721 atgaaaagaa gaaatatctc aaagccaact gccataaaaa cattttttac taattttaag
    12781 tgtagcataa tacctccaaa gatgagcttg atgtaaaaaa aggaatagga ctgatcgaaa
    12841 gagtaaggct tggacttggt aagccgaaca tcatttatgt taaagacttt atgagtatat
    12901 ttcaggtaaa agaaaatgac ttacagaagt caaaaaactt aacttcagaa gtaaaatatt
    12961 ttaacctcag aagtaaagaa aatgaacttc aagaggttaa gaaccttgac tctaactata
    13021 tagagaataa taagagtaag tatagtaaga gagaatatag ttttggtgaa aacggacttg
    13081 gaacatttca aaatgtgttt ttagctgctg aagatatatc ggatttacaa atcataatga
    13141 actcacagct tgagaattac attagacttc ctgcaaaact agaatcctag ttcatgattg
    13201 ataataccag cactcaaatt cattcgtaat ccgaagcgtt tacgatgatt tcgataggtt
    13261 gttgaaaaca ttttaaacgt ttttactttg gcaaagatgt tctcaacctt gcttctctcc
    13321 ttggatagcg catggttaca ggctttatct tcagctgtta gcggcttgag tttgctggat
    13381 ttacgtggag tttgtgcttg aggatatatc ttcatgagcc cttgataacc actgtcagcc
    13441 aagattttac cagcttgtcc gatatttctg cgactcattt tgaacaactt catatcataa
    13501 caaatagttc acagcgatat ccaaagaaac aattctccct tgacttgtga caatcgcttg
    13561 agccttcata gcgtggcatt tctttttacc agaatcattc gctaattctt tttttagggc
    13621 gattgatttg tacttctgtc gcatcaatca ttaccgtgtc ctcagagctg agaggagttc
    13681 ttgaaatcgt aacaccactt tgaacaagag ttacttcaac ccattggctc cgacggatta
    13741 agttgctttc gtggatacca aaatcagccg caatttcttc ataagtgcga tattctcgca
    13801 catattgaag agtagccata agaaggtctt ctaggcttaa tttaggtttt cgtccacctt
    13861 ttgcgtgttt aagttgataa gctgttttta atacagctaa catctcttca aaagtcgtgc
    13921 gctgaacacc aacaaaacgc ttaaatcgtg catcagttag ttgtttactt gcttcatcat
    13981 tcatagaact actataccat attttgtttc gcaggaagtc tattggaaag taagaaatat
    14041 tgaagctgag gctattagaa gaaattgtga gcgtggtgct attttttcag gtaaaataaa
    14101 atatcacgaa gattcacagt ttaaaggaga tcactatgtt gaatgttatg ctgttttaga
    14161 taatacggtt atagcaagag atagaataac agtccctatc gatccgttat gtggaaaaga
    14221 ttttatagag tagcatataa ttgattctta actggaatac tcactatctc tttacatcaa
    14281 gaaaatgact aaacagggaa gtttgccttc ttcccttttt ttgttatact agtagaagaa
    14341 aaaattagaa agatttgtgg gtgtcaaaca gtccagtggg gtgttttaat atggacttag
    14401 gtcccaccca aagaggtatt agtgtcgtgt ctcaatctta tatcaatgtt atcggtgctg
    14461 gtttggcagg ttctgaagca gcttaccaaa tcgcagagcg tggtattcca gttaaactat
    14521 atgaaatgcg tggtgtcaag tctacacccc agcataaaac agacaatttt gctgagttgg
    14581 tttgttccaa ttctttgcgt ggggatgctt tgacaaatgc agttggtctt ctcaaggaag
    14641 aaatgcgtcg cttgggttct gttatcttgg aatctgctga ggctacacgt gttcctgccg
    14701 gtggagccct tgcggtagat cgtgatggtt tctcccaaat ggtaaccgaa aaagtggtca
    14761 accacccctt gattgaagtg gtgcgtgatg aaattacaga attgccaaca gatgttatta
    14821 cagttgtggc gaccggtccc ttgactagcg atgccctggc tgaaaagatt catgccctta
    14881 atgatggcga tggcttctat ttctacgatg cagcagcgcc tattatcgat gtcaacacta
    14941 tcgatatgag taaggtctat ctcaaatctc gttatgataa gggagaagca gcctatctca
    15001 atgctccaat gaccaagcaa gagtttatgg atttccatga agccttggtc aatgcggaag
    15061 aagcaccgct caattctttt gaaaaagaaa agtactttga aggatgtatg ccaatcgaag
    15121 ttatggccaa acgtggcatt aaaactatgc tttatggccc tatgaagcca gtcggtcttg
    15181 agtatccgga cgactacaca ggacctcgtg atggagaatt taaaacacct tatgcggttg
    15241 tgcaacttcg tcaggataat gcagctggta gcctctacaa tatcgttggt ttccagaccc
    15301 acctcaaatg gggagaacaa aagcgtgtct tccaaatgat tccgggtctt gaaaatgcgg
    15361 agtttgtccg ttatggtgtc atgcatcgca attcttacat ggattcacca aatcttcttg
    15421 agcagactta ccgttccaag aaacaaccaa atctcttctt tgctggtcag atgacggggg
    15481 tggaaggcta tgttgagtcg gcggcttcgg gcttagttgc gggaattaac gcagctcgtc
    15541 tcttcaagga agaaagcgag gttattttcc ccgagacgac agcgattgga agcttagctc
    15601 attacattac ccatgccgac agcaaacatt tccaaccaat gaatgtcaat tttgggatca
    15661 tcaaggagtt ggaaggcgag cgtatccgtg ataagaaggc tcgttatgaa aaaattgcag
    15721 agcgtgccct tgccgactta gaggaatttt tgactgtcta atttttttga aagaattgct
    15781 catgatacta taaaaatctt agaaattgtg ataaaatagg taggatgaaa gaaggagagt
    15841 gaaaatggcg aatcccaagt ataaacgtat tttaatcaag ttatcaggtg aagcccttgc
    15901 cggtgaacgt ggcgtaggga ttgatatcca aacagttcaa acaatcgcaa aagagattca
    15961 agaagttcat agcttaggta tcgaaattgc ccttgttatt ggtggaggaa atctctggcg
    16021 tggagaacct gcagcagaag caggtatgga ccgtgttcag gcagattaca ctggaatgct
    16081 tgggactgtt atgaatgctc ttgtgatggc agattcattg caacaagttg gggttgatac
    16141 gcgtgtacaa acagctattg ctatgcaaca agtggcagag ccttatgtcc gtggacgtgc
    16201 ccttcgtcac cttgaaaaag gccgtatcgt tatctttggt gctggaattg gttcaccata
    16261 cttctcgaca gatacaacag cggcccttcg cgcagctgaa atcgaagcag atgccatcct
    16321 catggctaaa aatggcgtcg atggtgtgta caatgccgat cctaagaagg acaagacagc
    16381 cgttaagttt gaagaattga cccaccgtga tgttatcaac aaaggtcttc gtatcatgga
    16441 ctcaacagcc tcaaccctct caatggacaa cgacattgac ttggttgtct tcaacatgaa
    16501 ccaatcaggc aacatcaaac gtgtcgtatt tggtgaaaat atcggaacaa cagtttcaaa
    16561 taatatcgaa gaaaaggaat aagaaagaat atggctaaca taattattga aaaagctaaa
    16621 gagagaatga cccagtctca ccaatcactt gctcgtgaat ttggtggtat ccgtgctggt
    16681 cgtgccaatg caagcttgct tgaccgtgta catgtagaat actatggagt cgaaactcct
    16741 cttaaccaaa tcgcttcaat tacgattcca gaagcgcgtg ttttgttggt aacaccattt
    16801 gacaagtctt cattgaaaga catcgaacgt gccttgaacg cttctgatct tggtatcaca
    16861 ccggctaatg acggttctgt gattcgcttg gttatcccag ctcttacaga agaaactcgt
    16921 cgtgaccttg ctaaagaagt gaagaaggtc ggcgaaaatg ctaaagtggc tgtccgcaat
    16981 atccgtcgcg atgctatgga cgaagctaag aaacaagaaa aagcaaaaga aatcactgaa
    17041 gacgaattga agactcttga aaaagatatt caaaaagtaa cagacgatgc tgttaaacac
    17101 atcgacgaca tgactgctaa caaagagaaa gaacttttgg aagtctaaaa ataaacagaa
    17161 aaactcagtt ggcattgctg gctgagtttt attcgaaaga aggaaatatg aatacaaatc
    17221 ttgcaagttt tatcgttgga ctgatcatcg atgaaaatga ccgtttttac tttgtgcaaa
    17281 aggatggtca aacctatgct cttgctaagg aagagggcca acatacagta ggggatacgg
    17341 tcaaaggttt tgcatacacg gatatgaagc aaaaactccg cctgacaacc ttagaagtga
    17401 ctgccactca ggaccaattt ggttggggac gtgtcacaga ggttcgtaag gacttgggtg
    17461 tctttgtgga tacaggcctt cctgacaagg aaatcgttgt gtcactcgat attctccctg
    17521 agctcaagga actctggcct aagaagggcg accaactcta catccgtctt gaagtggata
    17581 agaaagaccg tatctggggc ctcttggctt atcaagaaga cttccaacgt ctcgctcgtc
    17641 ctgcctacaa caacatgcag aaccaaaact ggccagccat tgtttaccgt ctcaagctgt
    17701 caggaacttt tgtttaccta ccagaaaata atatgcttgg ttttattcat cctagcgagc
    17761 gttacgcaga gccacgtttg gggcaagtat tagatgcgcg cgttattggt ttccgtgaag
    17821 tggaccgcac tttgaacctc tccctcaaac cacgctcctt tgaaatgttg gaaaacgatg
    17881 ctcagatgat tttgacttat ttggaaagca atggcggttt catgacctta aatgacaagt
    17941 catctccaga cgacatcaag gcaacctttg gcatttctaa aggtcagttc aagaaagctt
    18001 taggtggtct tatgaaggct ggtaaaatca agcaggacca gtttggtaca gagttgattt
    18061 agggaggctt atgagaaaat cattttacac ttggctcatg accgagcgca atcctaaaag
    18121 taacagtccc aaagcaattt tggcagacct cgcttttgaa gaggcagctt ttccaaaaca
    18181 tacagatgat tttgatgagg tcagtcgctt tttggaggag catgccagtt tctcttttaa
    18241 cctaggagat tttgacagca tttggcagga atatctagaa cactagcatt tattcattgg
    18301 gtttgggcta gtaatttctc catccctctg ctataataaa aagaaataaa aggattagag
    18361 aggttcttta tttgaaggaa cattcaatag acattcaact gagtcatcca gatgacctgt
    18421 ttcatctttt tggttccaat gagcgccatc ttcgtttgat ggaagaagag ctggatgtgg
    18481 tgattcatgc tcgtacggag attgtccagg ttttgggaga agagtctgct tgtgaggaag
    18541 cccgtcaggt tattcaagct ctcatggtct tggtgaaccg tgggatgacc gttggtacgc
    18601 cagatgtggt gactgcaatt agcatggtca aaaacgatga aattgataag tttgtcgccc
    18661 tttacgaaga agaaattatc aaagataata ctgggaaacc gatccgcgtc aaaaccttag
    18721 gtcaaaaact ttatgtggac agtgtcaaac agcatgatgt gacctttgga attgggcctg
    18781 cagggacggg gaagaccttt cttgcagtta ctttggcagt gactgccctt aaacgtgggc
    18841 aagtcaagcg gattatcttg acacgtccag cggtggaagc aggagagagt ctaggttttc
    18901 ttccgggtga tcttaaggag aaggtagatc cttaccttag acctgtttat gatgccttgt
    18961 atcagattct ggggaaagac caaacaactc gactcatgga gcgtgaaatt atcgagattg
    19021 cgccccttgc ctatatgcgt ggacggacct tggatgatgc ctttgtcatt ctcgatgagg
    19081 cgcaaaatac gaccatcatg cagatgaaga tgttcttgac tcgtttaggt tttcattcta
    19141 agatgattgt caatggagat attagtcaga ttgacctgcc acgtaatgtc aagtccggtt
    19201 tgattgatgc tcaagagaaa ctcaagaaca tccatcagat tgactttgtt catttttcag
    19261 ccaaggatgt ggttcgccat cctgttgtcg ctcagattat ccgagcctat gaatattcta
    19321 ctgaagttgc acacgactga ttttgaggaa gttcgcctgc aaaagaatat acttttgaga
    19381 gaggaaaatc cagttgtata ggctaaaggt tttatccaaa ggtttgagac aacgattagg
    19441 cacgatggaa agaactttta tgtggctgat gacgatcagt gcatcttcct gtgtcataat
    19501 cacagggcac aagaaagtag gaatttgaaa agatgattga ccaactatct aagtattaca
    19561 gttgtaggat actaactgaa aaggatattc caagtatttt atctttatat gaaagtaatc
    19621 ctctgtattt tcagcattgt ccaccagagc caaattttgc aactgtaaaa gaggacatgc
    19681 tttgtctacc tgaaggtaaa gctaaggctg ataagttttt tgttggattt tggaatggat
    19741 ttgaccttgt ggctgttatg gattttgtct atgcatatcc tgatgaggag actgttttta
    19801 ttggtttgtt tatggttgat caagcctatc agaggaaagg gattggtagt catattgtga
    19861 cagaagcact agcttatttt gctaagaact ttcgaaaggc acgtttggct tatgttaagg
    19921 gaaatccgca atctcagcat ttttgggaaa agcagggctt taaatcaatt ggatgcgagg
    19981 ttaagcaaga actctatacg gttgttatcg ttgaacagag cctagaagat tagaaatggc
    20041 atcaagtaag aactatttgg aatttgtttt ggaacaatta tcaggattag atgatgtgac
    20101 ttaccgttcc atgatggggg agtatattct ttacttccgc ggcaagatta ttggcggcat
    20161 ttatgacgat cgcttttagt taaacccgtg caagcagtct tagataagat tgaccaatct
    20221 tcttttgagt ttccatacaa aggtgccaaa gaaatgattt gagtggaaga acttgataat
    20281 aagatgtttc tataagacct gattttagct atgtataacc aactgccaac gcccaaacct
    20341 aaaaagaaaa agcaagggtg aacgaagtaa aaaagaagtc tgctaaggcc ctgtctttgc
    20401 acgggtaaaa ttttatatat aaaaagaagc tgggactaaa gagctcaact tcctttggtt
    20461 tatataattg tcattacaag acgaagtggt tgggcgaaac tctgttgact ttattcaatt
    20521 tagagtttct tatgcacaat tgagtctgga acgaaagtct ccagttgcaa agtatacagt
    20581 acaataaacc aacgatgtaa tagctgatga cacaaagcac agtgggtagg acttgcgaag
    20641 tcaccctttt cttttcaaaa tttatactaa atcattgata tcagtgtagt cacgattaag
    20701 tccttgagca actggtaggc tagtcaagta accttgataa gtggtcacac cttgacgcaa
    20761 gccttcatct tcagagattg cttgtgcgaa tcctttgcca gccaaagctt cgatataagg
    20821 aagagtgaca ttggttaggg cgatggttga agtgcgggca accgcaccag ggatattggc
    20881 aacggcatag tggagaacac cgtgtttttc atagacgggt tcatcgtgcg ttgtcacacg
    20941 gtcagctgtt tcgataacgc caccttggtc aacagcaacg tcaacgatac agagcctgga
    21001 cgcatttgtt tgaccatctc atctgtcacc aattccggtg cttttgcacc agggatgaga
    21061 atggctccaa tcaccacatc agcatctctc atacttgctt caatgttgaa tgaattagat
    21121 ataagagttt gaatttgact tccaaagact tcttctagaa ctgagagacg cttggcacta
    21181 atatctaaaa tagtcacttg agcaccaaga ccaagggcga tgcgggcagc atgtgtaccg
    21241 acgacaccat caccgatgat agttactttt ccttttggaa cacctggtac accaccaggt
    21301 agaacaccag agccaccagc ttgcttagta aggaagtgag ctccgattta aacagccata
    21361 cgacctgcaa cctcactcat aggaacgagg agcggtagtt gtccttgatt gtcacgaaca
    21421 gtttcatagg caattcctgt tgtttttgct gctaacatag catctgctaa ttctggagca
    21481 gcggccatgt gcaagtaggt gaagagaaga agatcgtcgc gcaagtaacc gtattcagaa
    21541 cttaaaggtt cttttacttt cacaaccaac tctgctgccc aagcttcacc agcagtagcg
    21601 acaatctcag ctccttgctt ttgatagtca gcatcagtaa agccagaacc gagaccagca
    21661 tttgtttcga taaggacacg atgaccacga ctaactaagc tatgaacacc tgcaggtgtg
    21721 agggcgacac ggttttcgtt atttttaatt tcttttggga ttccgattaa cattgagata
    21781 acctaccttt caattgacgg tcttgttttg gttgtcacat tccagttcat aaatcaaaaa
    21841 tgtgacggtt tcattgtata tgaaaccgct tcaaaaatca agaaaaactt gtcatccaaa
    21901 ttttttatgc tagactagtg aaaatcaagc tttgattggt tggaaaagta tggaatcaat
    21961 ttttgtaaaa cttgcccagt atccgtctat agaaacggag cgtttattgc ttagacctgt
    22021 aactttggat gatgcggaag caatgtttga ctatgcctcg gacaagggta atacacgtta
    22081 cacttttcca accaatcaaa gcttggaaga aaccaagaat aacattgctc agttctattt
    22141 ggctaatccc ttgggacgtt ggggaataga actaaaaagc aatggtcagt ttattggaac
    22201 cattgacttg cacaagattg attctgttct taagaaggca gctattggct acattatcaa
    22261 taaaaagtat tggaatcaag gattaacgac agaagccaat cgtgctgtga ttgagctagc
    22321 ttttgagaag atagggatga ataagttgac tgcccttcac gataaggaca atcctgcgtc
    22381 aggaaaggtc atggagaaat caggcatgcg tttttcccat gcagaaccat atgcttgtat
    22441 ggaccagcat gaaaaagatc gaatcgttac aagagttcat tatgtcttga ccaaggaaga
    22501 ctattttgca aataaataag cagttgaaaa gaaatttttc gactgttttt tcttcctctt
    22561 acgaataatc taagagagga gaaaatatgg aagcaattat cgagaaaatc aaagagtata
    22621 aaatcatcgt catctgtact ggtctgggct tgcttgtagg cggatttttc ctgctaaaac
    22681 cagctccaca aacacctgtc aaagagacga atttgcaggc tgaagtcgca gctgtttcca
    22741 aggatttggt atccgaaaag gaagtgaaca aggaagaaaa ggaagaaccc cttgaacaag
    22801 atctaatcac agtagatgtc aaaagtgctg tcaaatcgcc agggatttat gacttgcctg
    22861 taggtagtcg agtcaatgat gctgttcaga aggctggtgg cttgacagag caagcagaca
    22921 gcaagtcgct caatctagct cagaaagtta gtgatgaggc tctggtttac gttcctacta
    22981 atggagaaga agcagttagc caacagactg gtttggggac agcttcttca ataagcaagg
    23041 aaaagaaggt caatctcaac aaggctagtc tggaagaact caagcaggtc aagggactgg
    23101 gaggaaaacg agctcaggac attatcgacc atcgtgaggc aaatggcaag ttcaaatcag
    23161 tagacgagct caagaaggtc tctggcattg gtgccaagac tatagaaaag ttaaaagact
    23221 atgttacagt ggattaagaa tttctctatt cccctaattt acctgagttt tctgttactt
    23281 tggctttact acgccatttt ttcaacatcc tatcttgctt tgttgggatt tgtttttctg
    23341 ctagtctgtc tctttttcca atttccttgg aaatctgcta gcaaagttct agtgatttgt
    23401 ggaatctttg gattttggtt tgtttttcaa aattggcaac agagtcaagc gagtcaaaat
    23461 ctggcggatt ctgttgaaag ggtacggatt ttgcctgata ctattaaggt taatggtgat
    23521 agtctatcct ttcgtggcaa gtctaacggt cgtgctttcc aagtctatta taaactccag
    23581 tccgaggagg agaaagaagc ctttcaagct ttaaccgacc tgcatgagat aggactagaa
    23641 gggaagcttt cggagccaga agggcagaga aattttggtg gctttaatta ccaagcctat
    23701 ctgaagactc agggaattta ccagactctc aatatcaaaa caatccagtc acttcaaaag
    23761 attggcagtt gggatatagg agaaaacttg tccagtttac gtcgaaaggc tgtggtttgg
    23821 attaagacgc actttccaga ccctatgcgc aattacatga caggactctt gctgggacat
    23881 ctggacaccg actttgagga gatgaatgag ctttattcca gtctaggaat tatccacctc
    23941 tttgccctat ctggcatgca ggtaggtttt ttcatgaatg gatttaagaa acttctcttg
    24001 cgattgggct tgacccaaga aaagttgaaa tggctgactt atcccttttc ccttatctat
    24061 gcgggactaa ctggattttc agcatcggtt attcgcagtc tcttgcaaaa gctactggct
    24121 caacatgggg ttaagggctt ggataatttt gccttgacgg tgcttgtcct ctttattgtc
    24181 atgccaaact ttttctttac agcaggagga gtcttgtcct gcgcttatgc ttttatcctg
    24241 accatgacca gcaaagaagg ggaggggctc aaggctgttg ctagtgaaag tctagtcatc
    24301 tccttgggca tattgcccat tctatccttc tattttgcgg aatttcaacc ttggtctatc
    24361 cttttgacct ttatcttttc ctttctattt gacttgacct tcttaccgct cttgtctatt
    24421 ttatttgtcc tttcctttct ctatccagtc attcagctga actttatctt tgaatggtta
    24481 gagggcatta ttcgcttggt ctcgcaggtg gcaagtagac cgcttgtctt tggacaaccc
    24541 aatacatggc ttttaatcct attgttaatt tctttggctt tggtctatga tttgaggaaa
    24601 aacattaaaa agctaacggt attgtgctta ttgattacag ggctctttct cctgaccaag
    24661 catccactgg aaaatgaaat caccatgctg gatgtggggc aaggagaaag tattttccta
    24721 cgggatgtaa ctgggaaaac cattctcata gatgtaggtg gtaaggcaga atcttataag
    24781 aaaatcaaaa aatggcaaga aaagatgacg accagcaatg cccagcgaag cttgataccc
    24841 tatctcaaaa gtcgaggagt agctaagatt gaccagctaa ttttgactaa cacggacaag
    24901 gagcatgttg gagatttgtc agagatgacc aaggctttcc atgtagggga gattctagta
    24961 tcaaaagaca gtctgaaaca gaaggaattt gtggcagaac tacaggcgac tcaaacaaag
    25021 gtgcgtagta tgatagtagg ggagaacttg cccatttttg gaagtcagtt agaagttcta
    25081 tctccaagga aaatgggaga tggaggacac gatgataccc tagttctgta tgggaaattc
    25141 ttggataagc aatttctctt cacgggaaat ttggaggaga aaggagagaa ggacttgctg
    25201 aagcactatc cagacttgaa agtaaatgtt ttgaaagcta gccaacatgg caataaaaaa
    25261 tcatcaagtc cagcctttct agaaaaactc aaaccagagc ttactcttat ctcagttgga
    25321 aagagcaatc gaatgaaact cccctatcag gaaacattga cacgactgga aggtatcaat
    25381 agcaaagttt atcgaactga ccagcaagga gctatacgtt ttaaggggtt ggatagttgg
    25441 aaaatcgaaa gtgttcgata ggaaggataa atgttgtaga ttagtgaaat aaactaaaaa
    25501 tttgttgcat aataatgata aaaatggtat aatgaaaacg tattcaatat tgaggatata
    25561 aaatcattaa aaatcagcaa aagttgtttt attagttagt ttataatcta ttggtcttct
    25621 tcagtccagt gtatctgctg tgacagtcac taaaagttac aagtatgatt ggaatacggt
    25681 tttggaatat agtaccaact atcacgacca tcagtatgct tggattccgg aatggtctcg
    25741 ttattacagc tattctgagt ataaagttgg cggaggctgg aactacgctc gttatgaggt
    25801 cataaactac tatactggag gttattaatc cttaaagagt gagaaaagga gggctggata
    25861 tgttaaatct tactcatgtt accttaaaaa cgcgacaagt catcttgcaa gatgcggatt
    25921 ttacctttaa aaagggtagg atttatggcc ttcttgctat caatggctcg ggaaagacga
    25981 cactattccg agctatgagc aagttgcttc cccttagtag tggacacatc gcagttcctc
    26041 cttctttgtt ttattatgag agcgttgaat ggctggatgg aaacttaagt gggatggact
    26101 accttcgtct cataaaaaac atctggaagt cagacctaaa cttgagagat gaaatcgcct
    26161 actgggaaat ggctgactat atcagtcttc ccatccgcaa gtattcctta gggatgaagc
    26221 aacgcttggt gattgctatg tattttctca gtcaggccaa atgctggctc atggatgaga
    26281 ttacaaatgg cttagacgag tattatcgac agaagttttt tgataggcta gcataaatcg
    26341 atagacaaga acagctggtt cttttaagtt cccactataa ggaagagttg gttgatatct
    26401 gcgatagagt agtaaccatt catcaggggc agatagaaga ggtttagttt atgaaagatg
    26461 ttagtctatt tttattgaaa aaagttttca aaagtcgttt aaactggatt atcttacttt
    26521 tatttgcatc tgtactcggt gttacctttt atttaaatag tcagactgca aactcagtca
    26581 gcttggagag agagttggaa actcgtcttg tagaccgtga gagagtcatc aatgaaaatg
    26641 aagagaaact ctcccaaatg tctgatacca gctcggagga ataccagttt gctaaaaata
    26701 atttagacgt gcaaaaaaat cttttgacgc gaaagacaga aattctgact ttattaaaag
    26761 aagggcgctg gaaagaggcc tactatctgc agtggcaaga tgaagagaag aattatgaat
    26821 ttgtatcaaa tgacccgact gctagctctg gcttaaaaat gggggttgac cgcgaacgga
    26881 agatttacca agccctgtat cccttgaaca taaaagcaca tactttggag tttccgaccc
    26941 acgggattga tcagattgtc tggattttag aggttatcat cccaagcttg tttgtggttg
    27001 cgattatttt tatgctaaca caactatttg cagaaagata tcaaaatcat ctggacacag
    27061 ctcacttata tcctgtttca aaagtgacat ttgcaatgtc ttctcttgga gttggagtgg
    27121 gctatgtaac tgtgctgttt atcggaatct gtggcttttc ttttctagtg ggaagtctga
    27181 taagtggttt tggacagtta gattatccct acccgattta tagcttagtg aatcaagagg
    27241 taactattgg aaagatgcag gatgtattat ttcctagctt gctcttagct ttcttagcct
    27301 ttatcgtcat tgtggaagtt gtgtacttga ttgcttactt tttcaagcaa aaaatgcctg
    27361 tcctctttct ttcactcatt gggattgttg gcttattgtt tggcatccaa acaattcagc
    27421 ctcttcaaag gattgcacat ctgattccct ttacttactt gcgttcagtg gagattttat
    27481 ctggaagatt atctaagcag attgataatg tcgatctaaa ttggagcatg gggatggtct
    27541 tacttccttg cctgattatc cttttgttag tggggattct atttatcgaa agatggggaa
    27601 gttcacgtaa aaaggaagtt tttaaagttt agttttccta tagggaagct aaagaaaaac
    27661 taacataggg agagaatcaa cttgattctc tctttttgat tcgaaaacca aaccaaaata
    27721 caaacacaaa cttttcaaaa aataactttt tatcttgaca agagctagaa aacttggtat
    27781 catataaaag ttgagaaaag cagaagtgag agcttctcgc cttgtgacat taagttgcct
    27841 ggccctacgg atgaaaagtt tcgaagaaac gctatcataa cgtgcgggct tgtatattta
    27901 cgagtccgct attgtttttc tctaataaaa caaaagaggt gaaaaccata gcaaagcaag
    27961 acttattcat caatgatgag attcgtgtac gtgaagttcg cttgattggt cttgagggag
    28021 aacagctagg catcaagcca ctcagtgaag cgcaagcttt ggctgataac gctaatgttg
    28081 acctagtatt gattcaaccc caagccaaac cgcctgttgc aaaaattatg gactacggta
    28141 agttcaaatt tgagtaccag aagaagcaaa aagaacaacg taaaaaacaa agcgttgtta
    28201 ctgtgaaaga agttcgtcta agtccaacta ttgacaaggg tgactttgat acaaaacttc
    28261 gtaatgcacg taagttcctt gaaaaaggaa ataaagttaa ggtatctatt cgctttaagg
    28321 gtcgtatgat tacccataaa gagattggtg caaaagtttt agccgagttt gctgaagcaa
    28381 ctcaagatat tgccatcatc gaacaacgtg ctaaaatgga tggacgtcaa atgttcatgc
    28441 aattggcgcc agcgactgac aaaaaataat tgtcagaaag ttaaataaag gagaaaaaat
    28501 catgccaaaa caaaaaacac accgcgcatc agctaaacgt ttcaaacgta caggttctgg
    28561 tggacttaaa cgtttccgtg cttacacttc tcaccgtttc cacggaaaaa ctaagaaaca
    28621 acgtcgtcat cttcgtaaag catctatggt gcattcagga gattacaaac gtatcaaagc
    28681 aatgcttact cgcttgaaat aatagcttga ctgtaagtaa acaattctag gaaatatttg
    28741 gaggaaataa aacatggcac gtgttaaagg tggcgttgta tcacgcaaac gtcgtaaacg
    28801 tattcttaaa ttagcaaaag gttactatgg agctaaacac atcttgttcc gtactgcaaa
    28861 agaacaagta atgaactctt actactatgc ataccgtgac cgtcgtcaga aaaaacgtga
    28921 cttccgcaaa ttgtggatta ctcgtatcaa tgcggcagct cgtatgaacg gactttcata
    28981 ctcacaattg atgcatggtt tgaaattggc tgagattgaa gttaaccgta aaatgcttgc
    29041 tgacttggct gttaacgatg cagtagcttt cacagctctt gcagatgcag ctaaagcaaa
    29101 acttggtaaa taacaactga tgagactgga acaggattgt ccagtctttt taaaaataaa
    29161 ggagaaagat atggcttcaa aaatgctaca cacttgctta cgagtagaaa atcttgaaaa
    29221 atcaatcgca ttttatcaag atgcttttgg ttttaaagaa ttgcgtcgca gagattttcc
    29281 agaccatgcc ttcacgattg tctatctagg tcttgagggt gatgactatg agttggagtt
    29341 gacttataac tacgatcacg gtccgtatgt ggttggtgat gggtttgccc atatcgccct
    29401 cagtacacct gatcttgagg cacttcatca agaacacagt gcaaaaggct atgaagtgac
    29461 tgagccaaat ggtctaccag gaactacacc aaactattac tttgtcaaag accctgatgg
    29521 atataaggtc gaagtcattc gtgaaaaata atttcgtgca agttaagtag aatattcgtt
    29581 ttctacttga ctttttttag ctgaaagagt atactaataa aaattcaacc tttaagggga
    29641 gtccagagag actcacaagg tgtcagataa aagaatagtg caattttcta gaggagactt
    29701 tttgagtgtg ctctcttgtg ttgtacgatt ttaactgagg ccttgcacta gcaaggtctt
    29761 ttctttatct ggtcccctta aaatttaagg aggaaaagtt atgaatccca catgtaagaa
    29821 gcgtttgggt gtcattcggt tggaaaccat gaaggtggtt gcacaagagg aaatcgcgcc
    29881 agcaatcttt gaattagtcc tagaaggaga aatggttgaa gccatgcgag caggccaatt
    29941 tcttcatctg cgtgtaccgg acgatgccca tctcttacgt cgtcctattt caatttcgtc
    30001 tattgacaag gcaaacaagc agtgtcacct catttatcgg attgagggag ctgggactgc
    30061 aattttttca accttaagtc agggagacac tcttgatgtg atggggcctc agggaaatgg
    30121 ttttgacttg tctgaccttg atgagcagaa tcaggttctc cttgttggtg gtgggattgg
    30181 tgttccaccc ttgcttgagg tggccaagga attgcatgaa cgtggagtga aagtagtgac
    30241 agtcctcggt tttgctaata aggatgctgt tattttgaaa acggaattgg ctcagtatgg
    30301 tcaggtcttt gtaacgacag atgatggttc ttatggcatc aagggaaatg tttccgttgt
    30361 tatcaatgat ttagatagtc agtttgatgc tgtttactcg tgtggggctc caggaatgat
    30421 gaagtatatc aatcaaacct ttgatgatca cccaagagcc tatttatctc tggaatctcg
    30481 tatggcttgt gggatgggag cttgctatgc ctgtgttcta aaagtaccag aaaacgagac
    30541 ggtcagccaa cgcgtctgtg aagatggtcc tgttttccgc acaggaacag ttgtattata
    30601 aggagaaaat tatgactaca aatcgattac aagtttctct acctggtttg gatttgaaaa
    30661 atccgattat tccagcatca ggctgttttg gctttggaca agagtatgcc aagtactatg
    30721 atttagacct tttaggttct attatgatca aggcgacaac ccttgaacca cgttttggga
    30781 atccaactcc aagagtggca gagacgcctg ctggtatgct caatgcaatt ggcttgcaaa
    30841 atcctggttt agaggttgtt ttggctgaaa agctaccttg gctggaaaga gaatatccaa
    30901 atcttcctat tattgccaat gtagctggtt tttcaaaaca agagtatgca gctgtttctc
    30961 atgggatttc caaggcaact aatgtaaaag ctatcgagct caatatttct tgtcccaatg
    31021 ttgaccactg taatcacgga cttttgattg gtcaagatcc agatttggct tatgatgtgg
    31081 tgaaagcagc tgtggaagcc tcagaagtgc cggtttatgt caaattaacc ccgagtgtga
    31141 ccgatatcgt tactgtcgca aaagctgcag aagatgcggg agcaagtggc ttgaccatga
    31201 tcaatactct ggttggaatg cgctttgacc tcaaaaccag aaaaccaatc ttggccaatg
    31261 gaacaggtgg aatgtcaggt ccagcagtct ttccagtagc cctcaaactc atccgccaag
    31321 tagcccaaac aacagacctg cctatcattg gaatgggggg agtggattcg gctgaagctg
    31381 ccctagaaat gtatctggct ggggcatctg ctatcggagt tggaacagct aactttacca
    31441 atccttatgc ctgccctgac atcatcgaaa atttaccaaa agtcatggat aaatacggta
    31501 ttagcagtct ggaagaactc cgtcaggaag taaaagagtc tctgaggtaa actgcaatca
    31561 atctgttctt gattttttat tagtttgtaa tatgaattta ggagaatttt ggtacaataa
    31621 aataaataag aacagaggaa gaaggttaat gaagaaagta agatttattt ttttagctct
    31681 gctatttttc ttagctagtc cagagggtgc aatggctagt gatggtactt ggcaaggaaa
    31741 acagtatctg aaagaagatg gcagtcaagc agcgaatgag tgggtttttg atactcatta
    31801 tcaatcttgg ttctatataa aagcagatgc taactatgct gaaaatgaat ggctaaagca
    31861 aggtgacgac tatttttacc tcaaatctgg tggctatatg gccaaatcag aatgggtaga
    31921 agacaaggga gccttttatt atcttgacca agatggaaag atgaaaagaa atgcttgggt
    31981 aggaacttcc tatgttggtg caataggtgc caaagtaata gaagactggg tctatgattc
    32041 tcaatacgat gcttggtttt atatcaaagc agatggacag cacgcagaga aagaatggct
    32101 ccaaattaaa gggaaggact attatttcaa atccggtggt tatctactga caagtcagtg
    32161 gattaatcaa gcttatgtga atgctagtgg tgtcaaagta cagcaaggtt ggctttttga
    32221 caaacaatac caatcttggt tttacatcaa agaaaatgga aactatgctg ataaagaatg
    32281 gattttcgag aatggtcact attattatct aaaatccggt ggctacatgg cagccaatga
    32341 atggatttgg gataaggaat cttggtttta cctcaagttt gatggcaaaa tggctgaaaa
    32401 agaatgggtc tacgattctc atagtcaagc ctggtactac ttcaaatccg gtggttacat
    32461 gacagccaat gaatggattt gggataagga atcttggttt tacctcaaat ctgatgggaa
    32521 aatagctgaa aaagaatggg tctacgattc tcatagtcaa gcttggtact acttcaaatc
    32581 cggtggttac atgacagcca atgaatggat ttgggataag gaatcttggt tttacctcaa
    32641 gtctgatggc aaaatggctg aaaaagaatg ggtctacgat tctcatagtc aagcctggta
    32701 ctacttcaaa tctggtggct acatggcgaa aaatgagaca gtagatggtt atcagcttgg
    32761 aagcgatggt aaatggcttg gaggaaaagc tacaaatgaa aatgctgctt actatcaagt
    32821 agtgcctgtt acagccaatg tttatgattc agatggtgaa aagctttcct atatatcgca
    32881 aggtagtgtc gtatggctag ataaggatag aaaaagtgat gacaagcgct tggctattac
    32941 tatttctggt ttgtcaggct atatgaaaac agaagattta caagcgctag atgctagtaa
    33001 ggactttatc ccttattatg agagtgatgg ccaccgtttt tatcactatg tggctcagaa
    33061 tgctagtatc ccagtagctt ctcatctttc tgatatggaa gtaggcaaga aatattattc
    33121 ggcagatggc ctgcattttg atggttttaa gcttgagaat cccttccttt tcaaagattt
    33181 aacagaggct acaaactaca gtgctgaaga attggataag gtatttagtt tgctaaacat
    33241 taacaatagc cttttggaga acaagggcgc tacttttaag gaagccgaag aacattacca
    33301 tatcaatgct ctttatctcc ttgcccatag tgccctagaa agtaactggg gaagaagtaa
    33361 aattgccaaa gataagaata atttctttgg cattacagcc tatgatacga ccccttacct
    33421 ttctgctaag acatttgatg atgtggataa gggaatttta ggtgcaacca agtggattaa
    33481 ggaaaattat atcgataggg gaagaacttt ccttggaaac aaggcttctg gtatgaatgt
    33541 ggaatatgct tcagaccctt attggggcga aaaaattgct agtgtgatga tgaaaatcaa
    33601 tgagaagcta ggtggcaaag attagtacta taagtgaata tgatttgagt gaatagtaag
    33661 ttaaaaatcc tgatttcaag taaaatcagg attttttcat ggatgcaatt tttttggagt
    33721 ctggtgtgac gcggagggtc ttttgtcctg tgtaagtgac aaagccgggt tttccaccag
    33781 ttggtttatt gagttttttg acttcaatca tatctacctg caccagattc gacaggcgcc
    33841 cttgagagaa gtaggcagct aactctgctg cgtctgtctt gactgcatca gatgggtcaa
    33901 gatttcctga gatgacaaca tggcttccag gaatgtcctt agcatggaac caaagttcct
    33961 ccttgcgggc cattttaaag gtcaattcct cattttgaag attgtttcgt ccgacataga
    34021 tgatggtttt gccatcgctt gctagatatt gttctagttt tttgcgtttc tggattttct
    34081 cccgttgttt tctgcggata aaacctgttt gaatcaattc ttcgcggatt tcagcgattt
    34141 cttccagtcc agcttggttg aggacggttt ctacactttc cagatagaga atagtggctt
    34201 tggtttcttc aatcaaatca gtcaagtatt tgacagcttc tttgagtttc tgataccgtt
    34261 taaaatagcg ttgggcattc tggttgggag tcagagcctt atcaagcgca atcatgatag
    34321 gttggttggt atagtagttg tctaggataa cctggtcttg gtcgttaggc acttggtgta
    34381 ggaaggttgt cagcaattct cctttttgac gaaattcttc agcgttgtct gtcgccagta
    34441 actctttttc ctgttttttg agtttgtgtc ggtttttctg aagttcattt tcaacacgac
    34501 gaatcagttc actggcctgc tgtttgacgc ggtcgcgctc agccttatcc ttatagtagg
    34561 tgtccaacaa atcagaaaga tttgcaaaag gctctcccac ctgatttgca aaaggaactg
    34621 gactgaagga agtctcagtc aagcatggct tggtttcttg attgaaaaaa tttcggaaag
    34681 cggaaagttt ttcactaacc agtatccttt ccaattcatt tgccgtatcg cgtcccagac
    34741 cttgaaagag gctttgaaga ttttttgctg ttagttcttg ggtttgcagg atttcaaaga
    34801 gcttttcatc tttgatagta aaaggattga gagattctgt acttggcgga gcgatatagg
    34861 tcgatcctgg aagtaaggtg cggtagctat tttgtgaaaa accgacgtgt ttgataactt
    34921 cgaggatttt atgactgctt ttatcgacca gtagaatatt actgtgtttc cccataattt
    34981 caataatcaa ggtagcctgg atatggtctc caatctcgtt tttattggaa actgtaattt
    35041 ccacaatacg gtcattttcc acttgctcaa tcgactcaat cagggccccc tgcaaatact
    35101 ttctcaaaac catgataaag gtagaaggtt gagctggatt ttcaaaagtc gtttgggtca
    35161 gctgaatgcg tccaaaaact ggatgggcag aaaggagcag gcgatggctt tggcgattgc
    35221 tgcggatttg caagaccaac tcttgttcaa aaggctgatt gattttctgg atgcgaccat
    35281 tcactaattc gcttcgcaat tcctcaacta tgtggtgtaa aaaaaatccg tcaaatgaca
    35341 tcgttctctc cttgtgattg tattccatag tattatatca aaaaggtaga ataaaatcat
    35401 ggaaatgtgg tataataaag ccaagtaaag agaaacgaga agcacatgta tattgaaatg
    35461 gtagatgaaa ctggtcaagt ttcaaaagaa atgttgcaac aaacccaaga aattttggaa
    35521 tttgcagccc aaaaattagg aaaagaagac aaggagatgg cagtcacttt tgtgaccaat
    35581 gagcgtagtc atgaacttaa tctggagtac cgtgacaccg accgtccgac agatgtcatc
    35641 agccttgagt ataaaccaga attggaaatt gcctttgacg aagaggattt gcttgaaaat
    35701 ccagaattgg cagagatgat gtctgagttt gatgcctata ttggggaatt gttcatctct
    35761 atcgataagg ctcatgagca ggccgaagaa tatggtcaca gctttgagcg tgagatgggc
    35821 ttcttggcag tacacggctt tttacatatt aacggctatg atcactatac tccggaagaa
    35881 gaagcggaga tgttcggttt acaagaagaa attttgacag cctatggact cacaagacaa
    35941 taaacgaaaa tggaaaaatc gtgacttgat atccagttta gaatttgcca tcacagggat
    36001 ttttactgct atcaaggaag aacgaaatat gcgaaaacat gcagtgacgg ctctagtggt
    36061 catccttgca ggttttgttt ttcaggtgtc acgaatcgaa tggctctttc ttctattgag
    36121 tattttcttg gtagtagcct ttgagattat caactctgct attgaaaatg tggtggattt
    36181 ggccagtcac tatcactttt ccatgctggc taaaaatgcc aaggatatgg cggccggcgc
    36241 ggtattagtg gtttctcttt tcgcagcctt aacaggcgca ttgatttttc tcccacgaat
    36301 ctgggattta ttattttaaa cagtaagagg aaattatgac ttttaaatca ggctttgtag
    36361 ccattttagg acgtcccaat gttgggaagt caaccttttt aaatcacgtt atggggcaaa
    36421 agattgccat catgagtgac aaggcgcaga caacgcgcaa taaaatcatg ggaatttaca
    36481 cgactgataa ggagcaaatt gtctttatcg acacaccagg gattcacaaa cctaaaacag
    36541 ctctcggaga tttcatggtt gagtctgcct acagtaccct tcgcgaagtg gacaccgttc
    36601 ttttcatggt gcctgctgat gaagcgcgtg gtaaggggga cgatatgatt atcgagcgtc
    36661 tcaaggctgc caaggttcct gtgattttgg tggtgaataa aatcgataag gtccatccag
    36721 accagctctt gtctcagatt gatgacttcc gtaatcaaat ggactttaag gaaattgttc
    36781 caatctcagc ccttcaggga aataacgtgt ctcgtctagt ggatattttg agtgaaaatc
    36841 tggatgaagg tttccaatat ttcccgtctg atcaaatcac agaccatcca gaacgtttct
    36901 tagtttcaga aatggttcgc gagaaagtct tgcacctaac tcgtgaagag attccgcatt
    36961 ctgtagcagt agttgttgac tctatgaaac gagacgaaga gacagacaag gttcacatcc
    37021 gtgcaaccat catggtcgag cgcgatagcc aaaaagggat tatcatcggt aaaggtggcg
    37081 ctatgcttaa gaaaatcggt agcatggccc gtcgtgatat cgaactcatg ctaggagaca
    37141 aggtcttcct agaaacctgg gtcaaggtca agaaaaactg gcgcgataaa aagctagatt
    37201 tggctgactt tggctataat gaaaaagaat actaagtaga ggtgggctca tgcctgcttc
    37261 ttgttttaca gaaggaggac ttatgcctga attacctgag gttgaaaccg tttgtcgtgg
    37321 cttagaaaaa ttgattatag gaaagaagat ttcgagtata gaaattcgct accccaagat
    37381 gattaagacg gatttggaag agtttcaaag ggaattgcct agtcagatta tcgagtcaat
    37441 gggacgtcgt ggaaaatatt tgctttttta tctgacagac aaggtcttga tttcccattt
    37501 gcggatggag ggcaagtatt tttactatcc agaccaagga cctgaacgca agcatgccca
    37561 tgttttcttt cattttgaag atggtggcac gcttgtttat gaggatgttc gcaagtttgg
    37621 aaccatggaa ctcttggtgc ctgacctttt agacgcctac tttatttcta aaaaattagg
    37681 tcctgaacca agcgaacaag actttgattt acaggtcttt caatctgccc ttgccaagtc
    37741 caaaaagcct atcaaatccc atctcctaga ccagaccttg gtagctggac ttggcaatat
    37801 ctatgtggat gaggttctct ggcgagctca ggttcatcca gctagatctt cccagacttt
    37861 gacagcagaa gaagcgacag ctattcatga ccaaaccatt gctgttttga gccaggctgt
    37921 tgaaaaaggc ggttccacca ttcggaccta taccaatgcc tttggggaag atggaagcat
    37981 gcaggatttt catcaggtct atgataagac tggtcaagaa tgtgtacgct gtggtaccat
    38041 cattgagaaa attcaactag gcggacgagg cacccacttt tgtccaaact gtcaaaggag
    38101 ggactgatga gaaaaatcat cggaatcact gggggaattg cctctggtaa gtcaactgtg
    38161 acaaattttc taagacagca aggctttcaa gtagtggatg ccgacgcagt cgtccaccaa
    38221 ctacagaaac ctggtggtcg tctgtttgag gctctagtac agcactttgg gcaagaaatc
    38281 attcttgaaa acggagaact caatcgccct ctcctagcta gtctcatctt ttcaaatcct
    38341 gaagagcaaa aatggtctaa gcaaattcaa ggggagatta tccgtgagga actggctact
    38401 ttgagagaac agttggctca gacagaagag attttcttca tggatattcc cctacttttt
    38461 gagcaggact acagcgattg gtttgctgag acttggttgg tctatgtgga ccgagatacc
    38521 caagtagaac gcttaatgaa aagggaccag ttgtccaagg atgaagctga gtctcgtctg
    38581 gcagcccagt ggcctttaga aaaaaagaaa gatttggcca gccaggttct tgataataat
    38641 ggcaatcaga accagcttct taatcaagtg catatccttc ttgagggagg taggcaagat
    38701 gacagagatt aactggaagg ataatctgcg cattgcctgg tttggtaatt ttctgacagg
    38761 agccagtatt tctttggttg taccttttat gcccatcttc gtggaaaatc taggtgtagg
    38821 gagtgagcaa gtcgcttttt atgcaggctt agcaatttct gtctctgcta tttccgcggc
    38881 gctcttttct cctatttggg gtattcttgc tgacaaatac ggccgaaaac ccatgatgat
    38941 tcgggcaggt cttgctatga ctatcactat gggaggcttg gcctttgtcc caaatatcta
    39001 ttggttaatc tttcttcgtt tactaaacgg tgtatttgca ggttttgttc ctaatgcaac
    39061 ggcactgata gccagtcagg ttccaaagga gaaatcaggc tctgccttag gtactttgtc
    39121 tacaggcgta gttgcaggca ctctaactgg tccctttatt ggtggcttta tcgcagaatt
    39181 atttggcatt cgtacagttt tcttactggt tggtagtttt ctatttttag ctgctatttt
    39241 gactatttgc tttatcaagg aagattttca accagtagcc aaggaaaagg ctattccaac
    39301 aaaggaatta tttacctcgg ttaaatatcc ctatcttttg ctcaatctct ttttaaccag
    39361 ttttgtcatc caattttcag ctcaatcgat tggccctatt ttggctcttt atgtacgcga
    39421 cttagggcag acagagaatc ttctttttgt ctctggtttg attgtgtcca gtatgggctt
    39481 ttccagcatg atgagtgcag gagtcatggg caagctaggt gacaaggtgg gcaatcatcg
    39541 tctcttggtt gtcgcccagt tttattcagt catcatctat ctcctctgtg ccaatgcctc
    39601 tagccccctt caactaggac tctatcgttt cctctttgga ttgggaaccg gtgccttgat
    39661 tcccggggtt aatgccctac tcagcaaaat gactcccaaa gccggcattt cgagggtctt
    39721 tgccttcaat caggtattct tttatctggg aggtgttgtt ggtcccatgg caggttctgc
    39781 agtagcaggt caatttggct accatgctgt cttttatgcg acgagccttt gtgttgcctt
    39841 tagttgtctc tttaacctga ttcaatttcg aacattatta aaagtaaagg aaatctagtg
    39901 cgagtaaaaa ttaatctcaa atgctcctct tgtggcagta tcaattacct aaccagtaaa
    39961 aattcaaaaa cccatccaga caagattgag gttttaaagt attgtcccaa ggaaagaaaa
    40021 gtaaccctac atcttgaatc taagtagatt ttatggtaaa ataataggga ttttaaggag
    40081 tttgatatgt ataacctatt attaaccatt ttattagtat tatctgttgt gattgtgatt
    40141 gcaattttca tgcaaccaac caaaaaccaa tccagcaatg tatttgatgc cagttcaggt
    40201 gatttgtttg aacgcagtaa agctcgcggt tttgaagctg taatgcagcg tttgacaggg
    40261 attttagtct ttttctggct agccattgcc ttagcattga cggtattatc aagtagataa
    40321 gaaaataatg ggcaggacta ggtctttgcc tctttttatt tttaaaggat gtttgagaag
    40381 gttttacagt aaaagaaaat taaaaaatct agaaagaaaa tatgaaagat agaataaaag
    40441 aatatttaca agacaaggga aaggtgactg ttaatgattt ggctcaggct ttgggaaaag
    40501 acagttccaa ggattttcgt gagttgatta aaaccttgtc cttaatggaa agaaagcacc
    40561 aaattcgttt tgaagaagat ggtagtctga cattagaaat taagaaaaaa catgagatta
    40621 ccctcaaggg gatttttcat gcccataaaa atggctttgg ctttgttagt ctggaaggcg
    40681 aggaggacga cctttttgta gggaaaaatg atgtcaacta tgctattgct ggtgataccg
    40741 tcgaggtagt gattaagaaa gtcgctgacc gcaataaggg aacagcagca gaagccaaaa
    40801 ttattgatat cctagaacac agtttgacaa cagttgtcgg gcaaatcgtt ctggatcagg
    40861 aaaaacctaa gtatgctggc tatattcgtt caaaaaatca gaaaatcagt caaccgattt
    40921 atgttaagaa accagcccta aaattagaag gaacagaagt tctcaaagtc tttatcgata
    40981 aatacccaag caagaaacat gatttctttg tcgcgagtgt tctcgatgtg gtgggacact
    41041 caacggatgt cggaattgat gttcttgagg ttttggaatc aatggacatt gtatccgagt
    41101 ttccagaagc tgttgttaag gaagcagaaa gtgtgcctga tgctccgtct caaaaggata
    41161 tggaaggtcg tctggatcta agagatgaaa ttacctttac cattgacggt gcggatgcca
    41221 aggacttgga cgatgcagtg catatcaagg ctctgaaaaa tggcaatctg gagcttgggg
    41281 ttcacatcgc agatgtttct tattatgtga ccgaggggtc tgcccttgac aaggaagccc
    41341 ttaaccgtgc gacttctgtt tacgtgacag accgagtggt gccaatgctt ccagaacgac
    41401 tatcaaatgg catctgctct ctcaatcccc aagttgactg cctgacccag tctgctatta
    41461 tggagattga taaacatggt cgtgtggtca actataccat tacacaaaca gttatcaaga
    41521 ccagttttcg tatgacctat agcgatgtca atgatatcct agctggcgat gaagaaaaga
    41581 gaaaaaaata tcataaaatt gtaccaagta tcgaactcat ggccaagctt catgaaactt
    41641 tagaaaacat gcgtgtgaaa cgtggagctc tcaattttga taccaatgaa gcgaagattt
    41701 tagtggataa acaaggtaag cctgttgata tcgttcttcg gcagcgtggt attgccgagc
    41761 ggatgattga gtcttttatg ttgatggcta atgaaacagt tgccgaacat ttcagcaagt
    41821 tggatttgcc ttttatctat cgaattcacg aggagcctaa ggctgaaaag gttcagaagt
    41881 ttattgatta tgcttcgagt tttggcttgc gcatttatgg gactgccagt gagattagtc
    41941 aggaggcact tcaagacatc atgcgtgctg ttgagggaga atcttatgca gatgtattgt
    42001 ccatgatgct tcttcgctct atgcagcagg ctcgttattc ggagcacaat tacggccact
    42061 atggactagc tgctgactat tatactcact ttaccagtcc aattcgtcgt tatccagacc
    42121 ttcttgttca ccgtatgatt cgggattacg gccgttctaa ggaaatagca gagcattttg
    42181 aacaagtgat tccagagatt gcgacccagt cttccaaccg tgaacgtcgt gccatagaag
    42241 ctgagcgtga agtcgaagcc atgaaaaagg ctgagtatat ggaagaatac gtgggtgaag
    42301 agtatgatgc agttgtatca agtattgtca aattcggtct ctttgtcgaa ttgccaaaca
    42361 cagttgaagg cttgattcac atcactaatc tacctgaatt ttatcatttc aatgagcgtg
    42421 atttgactct tcgtggagaa aaatcaggta tcactttccg agtgggtcag cagatccgta
    42481 tccgtgttga aagagcggat aaaatgactg gagagattga tttttcattc gtacctagtg
    42541 agtttgatgt gattgaaaaa ggcttgaaac agtctagtcg tagtggcaga gggcgtggtt
    42601 caaatcgtcg ttcggataag aaggaagaca agagaaaatc aggacgctca aatgataagc
    42661 gtaagcattc acaaaaagac aagaagaaaa aaggaaagaa acctttttac aaggaagtag
    42721 ctaagaaagg agccaagcat ggcaaagggc gagggaaagg tcgtcgcaca aaataaaaag
    42781 gcacgccacg actatacaat cgtagatacg ctagaggcag ggatggtcct gactggaact
    42841 gaaatcaaga gtgtacgagc tgctcgaatt aatctcaagg atggctttgc tcaagtgaaa
    42901 aatggagaag tttggctgag caatgttcat atcgcgcctt acgaagaggg caatatctgg
    42961 aaccaggaac cagaacgtcg tcgtaaactc ctgctccata aaaggcaaat tcaaaaattg
    43021 gaacaagaga ccaaagggac aggaatgacc ttagttcccc ttaaggtcta tataaaagat
    43081 ggctacgcta agcttctttt aggacttgcc aaagggaagc atgactatga caaacgggag
    43141 tctatcaaac gtcgtgagca aaatcgagat atcgcgcgtg tgatgaaagc tgttaatcag
    43201 cgataaaaag aggaattgaa aatggaaaaa ttagttgcct ataaacgcat gcctttgtgg
    43261 aataaacaaa caatgcctga agctgttcag caaaagcaca atacaaaagt tgggacttgg
    43321 gggaaaatta ctgtcttgaa gggagctctc aagtttattg aattgacaga agaaggggaa
    43381 gttctagctg aacacctctt tgaagcaggg gcagacaatc caatggccca acctcaagcc
    43441 tggcaccgag tggaagctgc cacagatgat gtggaatggt acttggaatt ttattgtaaa
    43501 cctgaggatt attttgctaa aaaatacaat accaatcctg ttcattcaga ggtcctagag
    43561 gccatggaga cagtgaaaca agggaaagct ttggatttgg gttgtggtca ggggcgtaat
    43621 tctctttttc tagcccagca agattttgat gtgacggctg tagatcaaaa tggactagct
    43681 cttgaaatct tgcaaagcat tgtggagcag gaagatttgg acatgcctgt tggcctttac
    43741 gatatcaatt cagctagcat tgaacaagaa tatgatttta tcgtttcaac agttgttctc
    43801 atgtttctac aagcggaccg cattccagct attattcaaa atatgcagga gaaaaccagt
    43861 gttggtggtt acaaccttat cgtttgtgcc atggacacgg aggattatcc ttgctcggtt
    43921 aacttcccat tcacctttaa agaaggagaa ctggcagact attacaagga ttgggaattg
    43981 gttaagtaca atgaaaatcc aggccatttg catcgtcgcg atgagaatgg caatcgtatt
    44041 caactacgct ttgcgacctt actagctaag aaaatcaagt aaacacacat gaagattagg
    44101 aattttcctg atcttttttc ttttttacga atgatataga aaaggaggga attcatgttt
    44161 gttgcgagag atgctagggg agaattggta aatgtgttag aggataaact tgagaagcaa
    44221 gcatacacct gcccagcttg tggaggccag ctccatttgc gtcaaggacc aagtgtacgg
    44281 acgcattttg cccataaatc cttaaaagac tgtgattttt tctttgaaaa tgaaagtcca
    44341 gaacacctgg ccaataagga atccctctat cactggttga aaaaagagac aaaggttcaa
    44401 ttagagtacc cgctttcaga acttaaacag attgcggatg tatttgtaaa tggcaatcta
    44461 gctctagaag ttcagtgtag tcccttgcct cagaaagtcc ttaaagagcg aagtgagggc
    44521 tatcgtagtc agggttacca agtactgtgg ttgctgggtc aaaaactgtg gctcaaggag
    44581 cgtttgactc gtctacagca aggttttctt tatttcagtc aaaacatggg cttttatgtt
    44641 tgggaattag acaaggaaaa acaagtttta agactcaaat acctgattta ccaggatctc
    44701 cgcggtaaac tccattatca aatcaaggaa ttttcctatg gtcaaggtag tttattggaa
    44761 atattgcgtc ttccctataa gagacaaaaa atatctcatt ttacagtttc tgaggacaag
    44821 gacatctgtc gctatatccg gcaacaactt tattatcaaa atctcttttg gatgaaagaa
    44881 caagcagaag cctatcaaaa gggagaaaat atcctgactt atggactgaa agaatggtat
    44941 ccacaaattc gaccaatagt gggcaaattt ttccagattg aacaagactt gactagctat
    45001 tatcagcact tttataccta ttaccaaaaa aatcctcaaa atgattggca aaagctttat
    45061 ccaccagcct tttatcagca atatttcttg aaaaatatgg tagaatagaa aggatggagg
    45121 aatctaatgg tattacaaag aaatgaaata aatgaaaaag atacatggga tctatcaacg
    45181 atctacccaa ctgaccaggc ttgggaagaa gccttaaaag atttaacaga acaattggag
    45241 acagtagccc agtatgaagg ccatctcttg gatagtgcgg ataacctact agaaatcact
    45301 gaattttctc ttgaaatgga acgccagatg gagaagcttt acgtttatgc tcatatgaag
    45361 aatgaccagg atacacgtga agctaagtat caagagtact atgccaaggc catgacactc
    45421 tacagccagt tagaccaagc cttttcattc tatgatcctg aatttatgga gattagcgaa
    45481 aagcagtatg ctgacttttt agaagctcaa ccaaagctgc aggtttatca acactatttt
    45541 gacaagctct tgaaaggcaa ggatcacgtt ctttcacaac gtgaagaaga attattggct
    45601 ggagctggag aaatctttgg ttcagcaagt gaaaccttcg ctatcttgga caatgcggat
    45661 attgtgttcc cttatgtcct agacgatgat ggtaaagaag ttcagctatc tcatgggact
    45721 tacacacgtt tgatggagtc taaaaaacgt gaggttcgcc gtggtgccta tcaagctctt
    45781 tatgcgactt acgaacaatt ccaacacacc tatgccaaaa ccttgcaaac caatgttaag
    45841 gtgcaaaatt accgtgctaa agttcgtaac tacaagagtg ctcgtcatgc agccctcgca
    45901 gcgaattttg ttccagaaag tgtttatgac aatttggtag cagcagttcg caagcatttg
    45961 ccactcttac atcgctatct tgagcttcgt tcaaaaatct tggggatttc agatctcaag
    46021 atgtacgatg tctacacacc gctttcatct gttgaataca gttttaccta ccaagaagcc
    46081 ttgaaaaaag cagaagatgc tttggtagtc ttgggtgagg attacttgag ccgtgttaaa
    46141 cgtgccttca gcgagcgttg gattgatgtt tacgaaaatc aaggcaagcg ttcaggtgcc
    46201 tactctggtg gttcttacga taccaatgcc tttatgcttc tcaactggca ggacaatctg
    46261 gacaatctct ttactcttgt tcatgaaaca ggtcacagta tgcattcaag ctatactcgt
    46321 gaaactcagc cttatgttta cggagattac tctatctttt tggctgagat tgcctcaact
    46381 accaatgaaa atatcttgac ggagaaatta ttggaagaag tggaagacga cgcaacacgc
    46441 tttgctattc tcaataactt cctagatggt ttccgtggaa cagttttccg ccaaactcaa
    46501 tttgctgagt ttgaacacgc cattcaccaa gcagatcaaa atggggaggt cttgacaagc
    46561 gatttcctaa ataaactcta cgcagacttg aaccaagagt attatggttt gagtaaggaa
    46621 gacaatcctg aaatccaata cgagtgggct cgtattccac acttatacta taactactat
    46681 gtatatcaat attcaactgg atttgcggcc gcctcagcct tggctgaaaa aattgtccat
    46741 ggtagtcaag aagaccgtga ccgctatatc gactacctca aggcaggtaa gtcggactat
    46801 ccacttaatg tcatgagaaa agctggtgtt gatatggaga aggaagacta cctcaacgat
    46861 gcctttgcag tctttgaacg ccgtttaaat gagtttgaag cccttgttga aaaattagga
    46921 ttggcataaa atggttgaat cgtatagtaa gaatgctaac cataacatgc gtcgtcctgt
    46981 cgtcaaagaa gaaattgtag acttgatgcg tcagcgtcaa aagcaagtca caggttcctt
    47041 gaaagaattg gaagactttg cccgcaagga aaatattcct attattcccc atgaaacggt
    47101 tgcttatttc cgttttctta tggaaaccat gcagcctaaa aatattctgg aaattgggac
    47161 ggctatcggt ttttcagctc tcttgatggc tgaacatgcg ccaaatgcta agattacaac
    47221 tattgatcgt aatccagaaa tgattggttt tgccaaggaa aattttgccc agtttgacag
    47281 tcgcaagcaa atcactctcc tagagggaga tgcggtggat gtcttatcta cactgacaga
    47341 gtcttatgat ttcgtcttta tggattctgc caagtctaaa tacatcgtct ttctgccaga
    47401 aatcctcaaa catttggaag ttggtggtgt ggttgtcttg gatgatattt ttcaaggtgg
    47461 tgatgttgcc aaggatatta tggaagtccg tcgtggtcag cgaaccattt atcgaggcct
    47521 tcaaaaatta tttgatgcaa ccttagacaa tccagaactc accgcaacat tagtgccttt
    47581 aggagatggt attctcatgc ttcgtaaaaa tgtagcagat gttcaactgt ctgaaagcga
    47641 atgattttca gaaaaattta agaaaaaata gtaaaataga tagagtaaca cttatctcaa
    47701 aggagtagac atgaagaaaa aattattggc aggtgccatc acactattat cagtagcaac
    47761 tttagcagct tgttcgaaag ggtcagaagg agcagacctt atcagcatga aaggggatgt
    47821 catcacagaa catcaatttt atgagcaagt gaaaaacaac ccttcagccc aacaagtctt
    47881 gttaaatatg accatccaaa aagtttttga aaaacaatat ggctcagagc ttgatgataa
    47941 agaggttgat gatactattg ccgaagaaaa aaaacaatat ggcgaaaact accaacgtgt
    48001 cttgtcacaa gcaggtatga ctcttgaaac acgtaaagct caaattcgta caagtaaatt
    48061 agttgagttg gcagttaaga aggtagcaga agctgaattg acagatgaag cctataagaa
    48121 agcctttgat gagtacactc cagatgtaac ggctcaaatc atccgtctta ataatgaaga
    48181 taaggccaaa gaagttctcg aaaaagccaa ggcagaaggt gctgattttg ctcaattagc
    48241 caaagataat tcaactgatg aaaaaacaaa agaaaatggt ggagaaatta cctttgattc
    48301 tgcttcaaca gaagtacctg agcaagtcaa aaaagccgct ttcgctttag atgtggatgg
    48361 tgtttctgat gtgattacag caactggcac acaagcctac agtagccaat attacattgt
    48421 aaaactcact aagaaaacag aaaaatcatc taatattgat gactacaaag aaaaattaaa
    48481 aactgttatc ttgactcaaa aacaaaatga ttcaacattt gttcaaagca ttatcggaaa
    48541 agaattgcaa gcagccaata tcaaggttaa ggaccaagcc ttccaaaata tctttaccca
    48601 atatatcggt ggtggagatt caagctcaag cagtagtaca tcaaacgaat agtccaaatc
    48661 aatgagtcag ggaaaaaact cgacttcagg aaaaaatgaa gcaaacattc ccacaataaa
    48721 acgcatagta caaggtttgt actgcccccc caaaagttag acaattaatt tatccgaagg
    48781 atttagttct gtattgcaca gagctaagtc cttttagttt tatcttaatt ctcttgttgt
    48841 tgtaataatc aatatagtct ataatggctt gttccaattg attaagtgat ttaaatgttt
    48901 tctcatagcc ataaaacatt tcggatttta aaataccaaa gaaagattcc atcctaccgt
    48961 tgtcttggct gtttcccttt cgtgacatag atgcttgaat tcccttactc tctaggaagc
    49021 gatgataaga atcgtgttgg tattgccagc cttggtcact atggagaatc gtattctcat
    49081 agtgcttctc tttgaatgcc tgttccaaca ttaacgatca atcaatttaa tcatgtacct
    49141 aagattagaa ttgtttatct caaatttatt tgaaag
//
LOCUS       DAVLVZ010000008        47960 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000008 DAVLVZ010000000
VERSION     DAVLVZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47960
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.008"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(480..2207)
                     /gene="ezrA"
                     /locus_tag="VYN77_000425"
     CDS             complement(480..2207)
                     /gene="ezrA"
                     /locus_tag="VYN77_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1557936.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKGIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2289..4235)
                     /gene="gyrB"
                     /locus_tag="VYN77_000426"
     CDS             complement(2289..4235)
                     /gene="gyrB"
                     /locus_tag="VYN77_000426"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1557937.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4250..4813)
                     /locus_tag="VYN77_000427"
     CDS             complement(4250..4813)
                     /locus_tag="VYN77_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1557938.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4898..5452)
                     /locus_tag="VYN77_000428"
     CDS             complement(4898..5452)
                     /locus_tag="VYN77_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1557939.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGNLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5463..6686)
                     /locus_tag="VYN77_000429"
     CDS             complement(5463..6686)
                     /locus_tag="VYN77_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1557940.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPRLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6723..9172)
                     /locus_tag="VYN77_000430"
                     /pseudo
     CDS             complement(6723..9172)
                     /locus_tag="VYN77_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
     gene            complement(9372..10301)
                     /locus_tag="VYN77_000431"
     CDS             complement(9372..10301)
                     /locus_tag="VYN77_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1557941.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQVFLLDEEVCKLHPKNRFLDTGFYDYDPDMVR"
     gene            10441..10728
                     /locus_tag="VYN77_000432"
     CDS             10441..10728
                     /locus_tag="VYN77_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1557942.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10762..12096)
                     /gene="ciaH"
                     /locus_tag="VYN77_000433"
     CDS             complement(10762..12096)
                     /gene="ciaH"
                     /locus_tag="VYN77_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1557943.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12086..12760)
                     /gene="ciaR"
                     /locus_tag="VYN77_000434"
     CDS             complement(12086..12760)
                     /gene="ciaR"
                     /locus_tag="VYN77_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1557944.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12869..15415)
                     /locus_tag="VYN77_000435"
     CDS             complement(12869..15415)
                     /locus_tag="VYN77_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1557945.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENNVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15538..15978)
                     /locus_tag="VYN77_000436"
     CDS             complement(15538..15978)
                     /locus_tag="VYN77_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1557946.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTSESKVVLEEFRKVYPL"
     gene            complement(15953..16822)
                     /locus_tag="VYN77_000437"
     CDS             complement(15953..16822)
                     /locus_tag="VYN77_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1557947.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDDDQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16800..17300)
                     /locus_tag="VYN77_000438"
     CDS             complement(16800..17300)
                     /locus_tag="VYN77_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1557948.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17311..18009)
                     /locus_tag="VYN77_000439"
     CDS             complement(17311..18009)
                     /locus_tag="VYN77_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1557949.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPSGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18002..18268)
                     /locus_tag="VYN77_000440"
     CDS             complement(18002..18268)
                     /locus_tag="VYN77_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1557950.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18334..19260)
                     /locus_tag="VYN77_000441"
     CDS             complement(18334..19260)
                     /locus_tag="VYN77_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1557951.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19335..20150)
                     /locus_tag="VYN77_000442"
     CDS             complement(19335..20150)
                     /locus_tag="VYN77_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557952.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20154..20474)
                     /locus_tag="VYN77_000443"
     CDS             complement(20154..20474)
                     /locus_tag="VYN77_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1557953.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20681..20761)
                     /locus_tag="VYN77_000444"
                     /pseudo
     CDS             complement(20681..20761)
                     /locus_tag="VYN77_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20805..22802)
                     /gene="metG"
                     /locus_tag="VYN77_000445"
     CDS             complement(20805..22802)
                     /gene="metG"
                     /locus_tag="VYN77_000445"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1557954.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22926..24185)
                     /locus_tag="VYN77_000446"
     CDS             complement(22926..24185)
                     /locus_tag="VYN77_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1557955.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24187..24888)
                     /locus_tag="VYN77_000447"
     CDS             complement(24187..24888)
                     /locus_tag="VYN77_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1557956.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24872..26071)
                     /locus_tag="VYN77_000448"
     CDS             complement(24872..26071)
                     /locus_tag="VYN77_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1557957.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADCHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26357..27703
                     /gene="gor"
                     /locus_tag="VYN77_000449"
     CDS             26357..27703
                     /gene="gor"
                     /locus_tag="VYN77_000449"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1557958.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNRESYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            27900..28436
                     /locus_tag="VYN77_000450"
     CDS             27900..28436
                     /locus_tag="VYN77_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1557959.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            28487..28573
                     /locus_tag="VYN77_000451"
                     /pseudo
     CDS             28487..28573
                     /locus_tag="VYN77_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(28642..28857)
                     /locus_tag="VYN77_000452"
     CDS             complement(28642..28857)
                     /locus_tag="VYN77_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1557960.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28955..29275
                     /locus_tag="VYN77_000453"
     CDS             28955..29275
                     /locus_tag="VYN77_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557961.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29305..29643)
                     /locus_tag="VYN77_000454"
     CDS             complement(29305..29643)
                     /locus_tag="VYN77_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557962.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29655..30374)
                     /gene="trmD"
                     /locus_tag="VYN77_000455"
     CDS             complement(29655..30374)
                     /gene="trmD"
                     /locus_tag="VYN77_000455"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1557963.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30364..30882)
                     /gene="rimM"
                     /locus_tag="VYN77_000456"
     CDS             complement(30364..30882)
                     /gene="rimM"
                     /locus_tag="VYN77_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1557964.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(30913..31701)
                     /locus_tag="VYN77_000457"
     CDS             complement(30913..31701)
                     /locus_tag="VYN77_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1557965.1"
                     /translation="MKRFEVSTEIGSLSVAYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(31805..32048)
                     /locus_tag="VYN77_000458"
                     /pseudo
     CDS             complement(31805..32048)
                     /locus_tag="VYN77_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            complement(32278..32517)
                     /locus_tag="VYN77_000459"
     CDS             complement(32278..32517)
                     /locus_tag="VYN77_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1557966.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32537..32809)
                     /gene="rpsP"
                     /locus_tag="VYN77_000460"
     CDS             complement(32537..32809)
                     /gene="rpsP"
                     /locus_tag="VYN77_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1557967.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            32979..33188
                     /locus_tag="VYN77_000461"
     CDS             32979..33188
                     /locus_tag="VYN77_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557968.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33664..33876
                     /locus_tag="VYN77_000462"
     CDS             33664..33876
                     /locus_tag="VYN77_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557969.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            33959..34762
                     /locus_tag="VYN77_000463"
     CDS             33959..34762
                     /locus_tag="VYN77_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1557970.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(34819..36360)
                     /locus_tag="VYN77_000464"
     CDS             complement(34819..36360)
                     /locus_tag="VYN77_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1557971.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHELDFYEGWIDQIWD
                     FNNLT"
     gene            complement(36362..36844)
                     /locus_tag="VYN77_000465"
     CDS             complement(36362..36844)
                     /locus_tag="VYN77_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1557972.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(36844..37929)
                     /gene="rlmN"
                     /locus_tag="VYN77_000466"
     CDS             complement(36844..37929)
                     /gene="rlmN"
                     /locus_tag="VYN77_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV1557973.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRGVTFEYIMLNEVNDGVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(37950..38480)
                     /locus_tag="VYN77_000467"
     CDS             complement(37950..38480)
                     /locus_tag="VYN77_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1557974.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(38636..39241)
                     /locus_tag="VYN77_000468"
     CDS             complement(38636..39241)
                     /locus_tag="VYN77_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1557975.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(39414..40451)
                     /locus_tag="VYN77_000469"
     CDS             complement(39414..40451)
                     /locus_tag="VYN77_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1557976.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(40485..41420)
                     /locus_tag="VYN77_000470"
     CDS             complement(40485..41420)
                     /locus_tag="VYN77_000470"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1557977.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(41973..43163)
                     /locus_tag="VYN77_000471"
     CDS             complement(41973..43163)
                     /locus_tag="VYN77_000471"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1557978.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(43378..44721)
                     /locus_tag="VYN77_000472"
     CDS             complement(43378..44721)
                     /locus_tag="VYN77_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1557979.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(44861..45676)
                     /locus_tag="VYN77_000473"
     CDS             complement(44861..45676)
                     /locus_tag="VYN77_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1557980.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEVVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            complement(45728..47908)
                     /locus_tag="VYN77_000474"
     CDS             complement(45728..47908)
                     /locus_tag="VYN77_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1557981.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEV
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     NAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
BASE COUNT        14227 a        10493 c         8677 g        14563 t
ORIGIN      
        1 atcctatctt ctagaatgtc ttccaaacga gaaaactctc gtaaacaaag aggttttaga
       61 ggcctattta ccgtgggact aaagttgtac aagaaaagtg caaataagaa atctccagat
      121 taggaactat ccgtgagttc tctagtctgg agatttttca atagacttcg ttattggacg
      181 gttaccaagt tactcaaagt acagatttga agctgcagat aagactgacg aaatcacttt
      241 aatactaaat gaaaatcaaa aagcaaacta agaagctagc ctcaggctgt acttgagtac
      301 ggtaaggcga cgctgacatg atttgaagag attttcgaag agtataaaac actgttttga
      361 ggtggtggat aaatcggctg ggtaaaaact caatctcagg agaaaatgaa gtaaatctcc
      421 ccacaactat agaaaaagat ttgattctgc tcctcacaca ataaaatctt tttcttttat
      481 taaaaacgaa tcgtttcacg tgttttctca tatgaggtaa caaagcgatt ggttacacca
      541 ggctctgcca cttccaatgc ttgagaaatc ttgtcaaatg aagcgtgata atcaaattct
      601 ttttcaaaaa tatctaaagc ttcgttaaat gcttcttgaa tgcgttcatc aaatgagcga
      661 tagcggttag aatattgcaa gagttgctct gtcaaagttg catattgtac aatattataa
      721 gtttccgttt ctaaagcttc catatcattc gttgcaattt caagaactcg ggtaacagat
      781 tcaatgttaa tcattttttg ttctaactca accattaaat cctcggtatt attgcttgcc
      841 gtaaagaata acttcaagaa agtttgtgga atacctggca gattgcgttt ttccatgtat
      901 cgcttgatag tatggagacg attgacataa acattggcct tttgacgtgc attaatatca
      961 tctttctcaa tttgtgtcag gcgctcacta actgaaattt gctcatcttc aatacctttt
     1021 agttgagttt gtaaatcctc aagattttct tcaagaactg aataagcttg ggttggttct
     1081 tcttgatttg aagttacctc aacaatagct gcctcaaaac tctctaattc tgtctgaata
     1141 cgacgaacat ggcttgcagc tgtctcagga agtaaatagg tcttgttcaa acgtgcaata
     1201 tcttctccca ataaagtatt attctctttc atatgttgaa ggtaagttgg aagagttgca
     1261 agtagatttt ccactacttt ctgagcagca atttctcgag taaaaatatc atacaaggca
     1321 ttgatttcct cttgtgcctg tccattctca tattcggcat tatccaattc caactgacga
     1381 atattctctt ggtttttctt gaatgcttca taaagcaagt ggaaacgcgc ttcaatatcc
     1441 gtttcaacaa aatgataatt agcatcaatt agtttacgat aaccggcttc caaatcctgt
     1501 aattgatctg gcaattctgt agaaagcgtc gtaaccaagg ctggaacacg atccacaata
     1561 tgacttaagg ccaaaatgtg attttctgta ttatccaaaa tcactgcggc ttccacaggg
     1621 tcacccgatg aattcaaggt tacaaattgt gaaaattcag attggatatt ttctaattgt
     1681 ttttcaattt catccaaggc ttgaccatac tgttctgaat tttcagcaac tctatgctga
     1741 agttcctcaa ataaatccaa agcatgaaga acacgaccac tatttttaga ttcttgcttc
     1801 tctaagtctg ccaaagcatt gcgaattgcc gcaatatctt cttcaatcaa agtaatttga
     1861 ctctcaattt ggtcaatttg atgactggcc ttgagaaaac gaaatgaatg gttatagcct
     1921 tctgcttcaa agagattatt ttcaatatcg gcaaaagagt tgagagataa atcgacccat
     1981 ttttgattcc attcacggaa agccacttga ctttgtccaa tcaagtgcat attttttaca
     2041 gcttctactt catcatttac tggaagattg tatagttctt cttttctttc ttctagcgcc
     2101 tctaatctcc cctcgtttcg cttacgtaga aagattgcca ctacataagc cagaactaaa
     2161 atgactgcaa ttgcaaccat taaataaatt agttgtccat tagacatatc aaactccttt
     2221 tttacttgaa acaatcgtaa cgattatatc atatttttta gaccaaggga actacttctc
     2281 cctatttttt agacatcaag tgtactatag acagcatttt cttcgataaa ctcacgacga
     2341 ggctctactc gatcccccat caacatatca aagattttat ctgcttctgc agcatcatct
     2401 acagaaactc tagccatcaa gcgatgttcg ggatccatgg ttgtttccca cagctgatga
     2461 tcgtccattt cacctagccc cttataacgc tgaatagtcg gtttggtacg accttcacta
     2521 taacgggcta aagcttcttg gagtttgatt tcttgatctg cacccggctg gatatattct
     2581 ttaatctcgc ttccaacctt gacaccatag attggtggtt gggcaatata aacataacca
     2641 gcttctagga ttggtttcat ataacgataa atcaaggtta aaagaagggt acgaatatgg
     2701 gctccatcga catcggcatc ggtcatcaaa acgagttttt ggtaacgggc tttcgaaaca
     2761 tcaaattctg cgccaaatcc tgttcccatg gctgtgaaaa gactacgaat ttcttcgtta
     2821 gctagaatct tatccatact tgctttttca acgttcaaaa tcttaccgcg aattggaagg
     2881 atagcctgaa actcacggtt acgaccagat ttggctgatc caccagctga gtctccttcg
     2941 acgatgaaga gttctgtttc agcagggtta ttagaagaac agtctgctag tttccctgga
     3001 aggttggaaa tttccaaacc agatttttta cgtgtgactt cacgcgcacg cttggcagcc
     3061 acacgagcct tggcagctaa aatccctttt tccacgatac gtttggcaat ctgtggattt
     3121 tccatgagga aatcggagaa ggcttcactg aagaggcgat tggtaatctt gaccacttcg
     3181 ctatttccca atttggtctt ggtttgtcct tcaaactgtg gatttgggtg tttaactgag
     3241 ataactgcag ttaagccttc gcgaacatct tcccctgtta aattatcttc attgtctttc
     3301 agtaacttat tcttacgagc ataatcattg ataacacgag tcagtgccgt acggaaacct
     3361 tgttcatgcg ttccaccttc atgggtatga atattattgg cgaaactcat gacattttca
     3421 tggtaacctg ttgtgtactg catggctacc tcaactgtga tatcatccat ctcaccgtct
     3481 gtatagattg gtgtatcaaa gattacatcc ttgttctcgt tgatatattc aacgtaacta
     3541 gcaatcccac cttcataatg ataatgcttg gtttgttcca aaccttggcg cttatcagtg
     3601 atagaaattt gaagaccgcg atttagaaag gccaactctt gaatccgttt atttaattta
     3661 tcaaaatcaa aggttgttgt ttcagtaaag atttttgggt ccggtatgaa gtgaaccgtt
     3721 gttccagttc tatctgtatc cccaaccacc tcaaggtctg caacaacatg accacgacgg
     3781 tattcttggt aatgaatctt accatttttg tgaacatgaa cgtctaattg agtggaaagg
     3841 gcattaacta ctgacgaccc caccccgtga agaccacctg aaaccttgta tccaccaccg
     3901 ccgaactttc ctccagcgtg aaggactgta aagacggtct caacagcagg acggcctgtt
     3961 ttttcctgaa tatcgactgg gataccacgc ccatcatcca caacagtaat cgaatcatct
     4021 ggctcaataa aaacttgaat atggctggca aatcctgcca aggcctcgtc aattgagtta
     4081 tcaacaattt cccagactag atggtgaaga ccttcttttg aggttgatcc aatgtacatc
     4141 cctggacgca tacgaacagc ctctaagccc tctaaaactt gaatttgact ggcatcataa
     4201 tcctgtgcct gcagattttt gatttcttct gtcatcttat tcctttttct tatatgtcta
     4261 atacttgtct taaattcacg atactggtaa acaagtgggt atcaagtcct gcagcttgac
     4321 ctgcttcgat atcaatcggc cgatcaccaa tgacaagacc agagctaatc tgatactttt
     4381 ctcttaaata aagcatggat tcgggatttg gctttctctt aaagcctgag ctagaagtca
     4441 ccacttctgt aaaataagct gctatagagg ttttttctaa aatttccaaa acctgatcat
     4501 ttcgatgaga gaccaaaaaa tgacggccac cttgatttga aatgacttcc aataggtcag
     4561 aaactccttc aaataaaatc gggtgttcaa gctctctggc ttcattttcc ttgtactttt
     4621 ctaaaaaatt ctctaaattg ggagcgaatg tctcaatcgc aaaaggagta gaaaccttta
     4681 aagcttgata gacactgtca tggtcttgtg tgataccata cagtgccaat gtttcaacaa
     4741 atgcagctgt tgaagtttca taattatcca gtaaagttcc acctaaatcc cagatgtaat
     4801 cgtgatattt catacccttc attatagcat ttttttatga aaaaagcgat gatttccctg
     4861 agttttccac ataaaaaacg agagaataac tcccgtttta ctgattttta ccaaagacat
     4921 ctcgatagag cattcctgtt cgtaaactct ctgcgtcccc tcctagttgc tccaccaact
     4981 cgtaggcaaa ggcaagggct gttgaaggac cccgactggt tgtcaactga ccatctacca
     5041 ctactgtttc cttgacgtag tgaccatcaa ggatttgctc ttgaacgccg tcataacaag
     5101 tgtatcgctt atttttcaat atctctgctt gattgagggc aattggtgcc gcacaaatgg
     5161 ctgctagttt cttcccttct tgctcgaagc tttgcaattc ttgaatcaag gtctgattat
     5221 cacgtaaatg tgcagaacca ggcatacctc caggaagaac aatcatatca tagtctgata
     5281 aatttccatc aaagacatga tctgctctta cttggattgc atgcgaaccc gttacttgct
     5341 cttcaaaacc aaccatatca catgtgatat tggctcgacg caagacatct acaactgtca
     5401 aggcttcaat ttcttcaaag ccctgagcta acataactgc tacttttacc atgattttct
     5461 ccttatttaa tttgttttaa tacaaccttt ttccgtttga atgggacttt tccgctcttt
     5521 tcttcagcta gattagtctg gtaactcatc gataaaagca aaccaacacc aatcagatta
     5581 ctgataatag ctgatccccc ttgcgaaatg aaaggcaagg gaatccccgt caaaggaagt
     5641 agtccagtca cagcaccgat attctcaaag atgtggaaga gcaacatcat aatcaaacct
     5701 gtggaaatat aagtgtagaa ctggttattt gatttaagag taatcttcaa catacggtaa
     5761 atcaacatga gatagagggc aataaccagg acagagccga taaagccaaa atcttctgca
     5821 ataaccgtaa aaatcatatc tgactctcga actgggataa gcagattcga agcattaaat
     5881 ccctgaccaa ataagccacc actcccaatg gcaatctgcc cttgagcctg ctggtaagtc
     5941 gttgtttggg caaactcaaa gggattgagc caagccaaaa tccgattgat ttggtaggtc
     6001 ggcattccaa tctggtgaag aaaagctcgt ccgtccttgc taataaagat agctaagaaa
     6061 ccagcaactc ctgttacagc agtcacaaat actgggataa taattttcca agaaacccct
     6121 gataataaaa cgattcctga gaaaatggct acaaaaacca aagccgtccc caagtcactt
     6181 tgaagtgcta aaagaactag gactggaatg gtaaagagaa tcatccagaa aattaacaaa
     6241 aagtccagcg gaaccgtgcg tctccattcc ttatgtttct ttgtaaattg gacaatgaca
     6301 cgagccaaca tgaggatata agatatcttc ataaattctg acggttggaa tagggtaatt
     6361 ccatttattg atacccagtt tttggcaccc gttgatgcaa ctaatcttgg attataaaat
     6421 acaatcggca agatcataag tcccaagcct aaaatatata gaaagggggt caccttccaa
     6481 agaaattctg tattaaagag catgaccaca aaaccaatca caagccccaa ggcaatccag
     6541 gcgacctgct gccctaaaat gggcagaata ttattgggat aatcatgact aacggctata
     6601 tagatagcca ccacaccgat gaccagtaga aaaaatactg gcaagagcaa actataatcg
     6661 actctagagt cgagagaacg tttcatatat actaacctta tactttcata caatactatt
     6721 tatcaaagtt cattaaaaaa tctatcaata gcctcgtcaa cttcggatcg agagatggtt
     6781 ttaacagttg cttcttctgc tagagatgct actatttgtt taccgtatcg ttttccgacg
     6841 attctcctat ccaaaataag agttaaggaa cgttggtatt cacgtctcat acttcttccc
     6901 aaagcctgtt ttaaacgaat aatggccatt ggcaattgat aatcataaaa ggcatttttc
     6961 ccttcttgat tcagttcttg attaatcttt ttcgtcaagg attcttgagg attttggaaa
     7021 ggaagtctcg gtacaacttg aatcacaaaa ggatggcttg aaaaatcaac tccctcccag
     7081 aaacttgctg caccaagcaa gatttgttgt tcaccttttt caaagcgttt ctttagctga
     7141 tgaacatccc catttttata ctgggccaag tggctaactg taagtaaatc cgatactgct
     7201 agaagcatgt ctttagcggt aaagagaacc aaaatcggtt gctggaaagc ttgaatttcc
     7261 actagtaaag cagctacctc tctggcatag acttctaagg aggtttctgt taccagaggg
     7321 aaatctttga ccataaccac ttcttgttcc tgttttcccc gagattcaat cttgacaaat
     7381 ttggcttcag gatagcctaa aaggtctgcc aaagaaaccc tctgactaat ctcaagagta
     7441 gccgatactc ccaggacttg gcaactctca ggaagtaaag aggacagaag agtacggcct
     7501 gattttgtag aagaaatctg aattttcttt tgactcgttt cagttacttc aagccagtaa
     7561 tcaccttcag ctgtaaaata gcgaaccagc tcatcaaagc cttccacttc taattctgaa
     7621 aaatactgat ggagattgtc cagagaatct aagatatttt tcctagattt gccagactga
     7681 aattgttcta tcaagtagag acactcaaag cgaatacttt ctagtatccg ttgttgaacc
     7741 ctgttttctt ctcctactaa agccttatca attaaatcga taatagattg tatatcgtag
     7801 gtctcttgaa gcagattttc tagagctaac aaaatctttt ggacttcatc aataatcagt
     7861 aaacggtcac tgacaaattc aggattatct tcaagtctgg tcacgagata ggcatgatta
     7921 gtcactaaaa gcttgcaagt ctctgccctt tcttgactac gtttccaaaa atcttccgtc
     7981 acaaataagc tctgggatga taaattccca tcatgacgaa ggtctgctag aaaatgttgg
     8041 taacggtaga gttgcccgat ttcatccaaa tctcctgtct ctgtctcagt aagccagacc
     8101 aagacttgca ttttaaagcg tctaaataag cgattttcat cattttcctg caaggaatga
     8161 taaaaggcat ccaacttcag ataattttgt ggtcccttta agctatgaat atctgtatgg
     8221 aacacttcct tgagacgttt accttcttct tccatgattt gattttgaag aatctttgtc
     8281 ggaacactaa gaacaatttg tcgctctttg gattgagata aagcgggtaa gagatagcca
     8341 taggttttcc caatccctgt cggcgcttga atcagagaga caggttcatc tttcaatagc
     8401 aagccaacct ctttagcaaa actttcttgt tcctccctca cttcaaggtt caacagagaa
     8461 atatttttag agaagtcttg agatagtttt cgtagctcca gagaagctgc cgttttctta
     8521 aaatacagac cttgaacttg gaccaagtct ggagaactca ggatagattg gttgcgataa
     8581 gtttcctcaa taaccaggta ggactcatat aggagagcgt cagccatttc cagcaagcgt
     8641 tccaagagac ctttaggaag ctgggtcatc ttttcccgta aaaaagaagt aattctgctg
     8701 tagcttgggc atctgaaagg gctgtgtgtg cgtgtttaag aggaattcct aattctcgac
     8761 acaaaatcgg caagctatat ttttccagtt cagggaaaaa gacctgggcc aattcgaccg
     8821 tatcaacacg agggtttctt agctcatagc cttcaaaaaa taaattttcc gccaagagat
     8881 tagcatcaaa ctgaacatta tgggctacaa aaatcccatc ctccaccaag tcaaatattt
     8941 ttctggcaac ttgcgaaaaa tcaggtgctt gcgccagacg ttggtctgtc aatcctgtca
     9001 gttctttgat atgagcatcc aagggttcat gtggattgac atccgtcgta tagtgatcga
     9061 cgatttctcc gtcctcaatc acgacaattc ccacttggat aattttagcc ttactacctg
     9121 tgctagttgc ctctaaatcc accacaacat agcgattacc aatcgttttc attataaaaa
     9181 attataccaa aaaaagggcc atttggcacc tgcaaacata ggataatttt caaactcaag
     9241 aacactactt ttcttaaaaa tcccatcgaa tagagcattc tagcctgctg atttttgtaa
     9301 aatagtgaag aaatacgaaa aattgagaga agaaatgttt gcatcttcct tctctcaacg
     9361 aatcatgtat tttatctaac catgtcagga tcgtagtcat aaaatcctgt atcaaggaaa
     9421 cggtttttag ggtgaagttt acaaacttct tcatccagca agaagacttg atcatggcta
     9481 aagttgccct cttgaatcaa gttacgcgct tggataaaga gttttgcaat ctcaacaaag
     9541 ttctgaccag gagtgtagag cccttctagt ttccagcgag taaagccatg ctccaccaat
     9601 tctgttaatt tgatcatcaa atcaaggtca ttgttggcaa agatatgggt cccatgatta
     9661 tcttcaaaaa tggaatagtg gctctctgga tcacttggct cagccaagaa gaggtcacgt
     9721 ttatgcgtct tttcatcatc gatatgtgta aagttatagt agttttgcaa gagtggacgt
     9781 ttagaatgat ggatgacgct agcaccgtaa accaaaactt cagcaggaat ttccaaaatc
     9841 tctggcattt tgaaaagttc agctgatgga atttcacgcg ccaaaacagc ctcagatgcg
     9901 ccagcctttt gtccccagaa gttaatctga cgactgctag ttaccatggt tgaagcatcg
     9961 tagatggtct taaatgaata accatcgcgg ttaactacgt aaaagacgcc tgcatcccca
    10021 atcgtaatat agtctgtctt gatttcttcc aagaagtcta agaaaggctt gatacggtcc
    10081 atcatatctt ggtgcatgag agcattgacc gcaacgagca attccttacc agcatcatga
    10141 accaacttag cgatttcacg taattggtca taactaaagg tcgttggcag acgaaggcca
    10201 aaatctttct cacctacata gatacggtct acgccagctt cgagtagttg ttcaacttgt
    10261 tcaatacttt cagcagttgc tgtaatgaca atcttttcca taagaaaaat tataccacat
    10321 ttctccaaaa tcagccatgt tttgaaaatg aaaagggact ttattctttt tgtaaccttt
    10381 ctgtaataat tctctgccga aaaaatgata aaataggtat gtactgttaa ggagagaatc
    10441 atgcccgtaa gaaaattaca atcctatgag gtagactatc aagaagaatt aaaccagcag
    10501 cttcctcatt accaagctta tacacctgaa gcgcaatctg atgccaatct caaggaaatc
    10561 ctatttttta ttaatatcgc tgttttttgt atctgtattg ctatctttag ttttatcttt
    10621 ttagcattaa aattatcaac tgctcttgcc tttgccgcag caatcggatt cagcttactt
    10681 gttttaaaag ttcaacggtc tattatcaaa cgaaaacgta gaagataaat ccaaaatatc
    10741 gccccaattg gagcgatatt tttatttttt ctttttagat ggtgtctgaa tggcaatctt
    10801 cacttcaaag attgttccct tgggtttatt atctttgaca gtaacagttc cttttagagc
    10861 atctacaatt tgcttggcta gggataatcc taatccaaaa ccaccttttt gccgggttct
    10921 agccttgtct actcgataaa aacggtcaaa aattttcttt ttatcttctg tcgaaatacc
    10981 gattccatta tcagaaacaa gtaaataaag attgcgatcg gtcgccgaga taagaaaatc
    11041 aatttcacca tcctcctcag tatacttgac ggcattatcg aaaagaatgg tcatcagttg
    11101 tttcagaaga agctgatctg tgacaattgt tcgatggata cgattttcaa aacggaagac
    11161 acgattattt tccgaagcaa tcatctcata gtttgtgaaa gttgtattaa aaaagctagt
    11221 tggaacttct gcaagctccg gcttaatccc atcatctctc cgagctaagt tcagcaagct
    11281 tgtcgttaaa aaacgcatat ttcggacttc ttccaaactc gatgcaatgc tttcgctcac
    11341 atccataatg gtagcttctg gcttacgaaa aagggtctct aagcgatttt gcaaaactgc
    11401 gagtggagtt cgtaactcat gactggcatt ttccacaaaa gactgttgct tctgcatact
    11461 ctcaagcagg ggcctaacac tgaccctagc tagatagaga ctggcaagta aagacaaaat
    11521 ccagaaacta gccatcacga ccacaatcaa ttgctcatgc ttttgactag cctgctccaa
    11581 ctgactggta ttaatcaaga cagcagcata cttgatattg gttgaaaccg aactaatatt
    11641 ggtctccatc aaaatcacac gatagatttc ttcctgccca tagctattaa aaacctgaat
    11701 ctggtagata tgtcctagtt ctttcttttc taacttaatc ttatccaagc ccaaaaatcg
    11761 atttccagaa agaagttgag taaagtcttt atcaaagaga atgacttccg tattggaact
    11821 gacattaggt tttatttcta ctttactagc gtccgcccta gcattttcta aatctttaat
    11881 ctcttctgtt gccctattta tagccagctg aataactgct tgaggatttt cactcagtcc
    11941 atgaagctta tcgtccaccg aagtataaag actcgaatgc atgacttgta aaataatcag
    12001 agtcattgta gaaaaaatca gggtgaagac accgaagttg cggataaaat aactaaagtc
    12061 atccgcatac catgtttttt taagtttact gaacatcttt taaaagatac ccaacactac
    12121 gcaaggtttg caaattctct gcaaaagtgg ttccctttaa tttcttacgg acttttgaaa
    12181 catagacttc gacaaccgaa atcgttgtat cactatcaaa tccccataga cggtcaaaaa
    12241 tctgcgtctt aggcaaaatc acattttgat tttgaaggaa ataaactagt aaatcgaact
    12301 ctttccccag caattcgaca ggagtatctt caactttaac ggtattggtt gataaattaa
    12361 ccacgatatt cccataagtc aaggtgtttt cattaaactt ccctgaacgt ttgagaaggg
    12421 cctgaattcg cattttaagt tcttctaggt agaaaggttt ggtcagataa tcatccgctc
    12481 ccagttcaaa tccatgtccc ttgtcatcca aactttcctt ggcagtcata atcagaactg
    12541 gtgtcgtaat tcccttttca cgcaattctt ttaagacttg gaaaccattt ttttctggca
    12601 acatcaaatc cagcaaaatc aagtcataga caccactctc agcttcgtag agaccttctt
    12661 ctccatcaaa tacctgcata acatccgcaa aatcgtctaa aaagtcaaat actgaatttg
    12721 acagacctag gtcatcctca accaataaga tttttatcat gagaaactcc tccttattaa
    12781 aactattata ccaaatttgc cttaaaaaaa actcaactct ctgcatttta catgagatag
    12841 ctgagttttc tttttatttt aggcttattt atgcatttcc gtattgaaga acaactgctt
    12901 cgactgcagc tttttcacgg ctaatcaagt caacacgtgc tgcaatttcc ttgattccca
    12961 taccgatgtt acggctaaga gcaaggtcag aaagttgcgg ctcaaagaac tccttgtatt
    13021 ccgccaagcg ttgctgagtc ttaaatacat gagcaggaag gataacaaag ctatcaaagc
    13081 tcatatctcc tccaagggct gccttaatcc aagcccagtt ttcacgcgcc caagaccaag
    13141 ctgttttctg agttgcttga tgagctagga attggtaata ccaagcagac aagtcctgtg
    13201 gtttgaccac aaatttgtcc ttccaagaag taatcaagtt ttggatatta tccgcatctg
    13261 tactgtatgc aagagctgct gccaactggc gtttaaagac agcatctgtt gcgtgagtat
    13321 aagtatcaag ataaagtgct aacaagtctt tagtctcatg atgtttcatc tcattaatca
    13381 gaacttgtga acgaatagct gctgggagtt ctgcaagatt ctccttgtgt gttgcgaaga
    13441 tttggctagc gacttgacga gcttctgcat catttgagcg aatcatcatc gaaacagcca
    13501 gctgacgaac caattcatcc tcatctgatt ctccgtcttt agcttcaaaa ccaagacggt
    13561 catagttatg acgagccaat ttagcaacca gtcctttgaa ggctgtttca gcatccgttc
    13621 cttcatcaat aaagcgctca agggctgaaa tcacttgaga aacagctgaa accaccagat
    13681 aagactcttc cttagcaagt ttatcaagaa ctggaagcaa gtctgcataa gaaatgtgcc
    13741 ctgcctcagc caacaaacga cgttcttgaa caatttgcag tttgcttgtg ttatcaagtg
    13801 tctctagctc agcaagaaca gcttctaaca agtctccttg atagtcggta atatagtggg
    13861 cagtattttc agtgttgaga cgaagagctc cttcattttc agcaagaaga gctgcgtagc
    13921 cagggatttc gatactttca gtttcgagtg tatcaggcaa gcctttccag ttgctattga
    13981 ggggcaccac ccagagacgg ttcttgtctt cgttctcacc gatgaagaat tgtttttgtg
    14041 aaatcttcaa gacattattt tcaactttga cagtaagaac tgggtaacca ggctgttcca
    14101 accaagaatc catgaaggct gcgacatcac gtcctgacgc ttgaccaagg gcatcccaaa
    14161 ggtcactacc aatggtgttg ctgtattggt gtttttcaaa gtaggcatgc aaacctttag
    14221 caaaatcagc atctcctagc caacggcgaa gcatgtgcat gagacggctt cctttggcat
    14281 agacgatagc gccgtcaaag agtgtattga tttcatctgg atgtttaact tcgacgtgga
    14341 cagactgaac gccatcagta gcgtcacgtt caagagcaag aggtactcca cctgtttgga
    14401 aatcttcaaa gatattccag cttggttcga tggtatccac acagacgtat tccatcatat
    14461 tagcgaaact ttcattgagc caaaggtcat cccaccattt catagtcacg aggttcccaa
    14521 accattggtg agccaattca tgggccacaa caagggcaac ttgttgacgg ctagcaaatg
    14581 tagagttctc atcgacaacc aagtaaactt cacggtaggt cacaagaccc cagttttcca
    14641 tagcaccagc tgagaagtca ggaagggcga tgtggagaga ttgaggaatt ggatacttaa
    14701 ctccatagta atcttcgtaa aactcgatag agcgaacagc gatatccagt gagaaatcaa
    14761 gatttgaaag tggatgtgct ttggttgagt agacacctac cagggtacca tttttagttt
    14821 tagcggtcac cccttgcaaa tcaccagcaa caaaggccaa caagtaagaa gacatgcgag
    14881 gtgttgtctc aaacttccag atacctgttt ccttacggtt ttcaacatcg atttctggca
    14941 tgtttgacaa ggccaattca ccttctgctt ggtcaaagcg aagagagagg tcaaaagttg
    15001 ctttggcttc aggctcatcc acacatggga aagcttcgcg cgcaaaatgg ctctcgaact
    15061 gagtagacaa gacctccttc ttgactccat caactgtata ataagaaggg taaatccctg
    15121 tcatgttgtc tgtaatttta ccagaaaagg caagaaccaa ttcaacttga ccagcctcag
    15181 ccaattcgat atgaagggct tcattgtcat ggtcaactgt aaatggacga gcttgacctg
    15241 caacttctac agaggtgatt tccaagtctt tttggtggag ggagatgcgg tcactctgtg
    15301 cttgaccagt gatggtcact ttcccagaaa aagtcttggt ctcacgactc aaatctaaaa
    15361 ataaatcata atgttcagga acaaattgct taataaaatg ttcaactgct tgcatagttt
    15421 tctcctattc taagtttaag agctgctgct cttcttcaaa caaacttatt atatcatatt
    15481 ttgcttgaaa aaaaaggatt ggacagcgat ttccaaaact ttcttccttc taatagtcta
    15541 cagtgggtag accttgcgaa attcttctaa aacaaccttg ctttcagatg taaaagtcag
    15601 ccctacttca cccaaacagt ctgtgaaaaa gtcctcatga acctggcgcc aataggcaag
    15661 tgatttgtct ccttcacctt ccttaaaggc atgttgcgca gaaacttgat tgaagagttc
    15721 aacagaaacc tttgtaattt cgacaatgca gacagactga ttttgactat ctaaaatgat
    15781 atcgaaggtc cctacttggg gaagtggttc gtcttctagt acatagaggt cataggctga
    15841 tgctgttgct gtcttttctc ctttaaacac caaatccgct aaaagatctg gttcaactcc
    15901 aaaagcccaa gcatcgattt catctccgat caaaggattg atttgcttgt atttattcca
    15961 catttcttgc ggtatcatgg gtactccttt gtaatttttt actttcttct tttatgtgtt
    16021 taagatgatc tggatggtca atctctaaat caaaaatctc tggaatagaa ctgtagtgga
    16081 taatgcactt gatacccaac tgattcattt tttgtatgaa agaagtattc agatagcctg
    16141 ctacagcaaa atcaatcttg ttctttcttg ctttatcctg catatctctt agcatatcta
    16201 acattattgg actttccata tcatgccatt gactgtttct catagtcgca aaaacaaagg
    16261 aagtcaaatc attcattcca actacaatct ttgaaatgcc cgtttccagt atactagata
    16321 agtcaaaata cgctgacggt aattcaatca tcgttccgac tttcccagta aaaccctgct
    16381 gacgcaatac tgtaatagct tgttttaatt ggtcgtcatc attgacaaaa ggaaagataa
    16441 cagatagatt ggggttggtt tgataaactt ctgtaacgac atgtgcttca gcctgaaatt
    16501 catccaaaca cgccagtaaa cgcctggttc ctctatagcc aaacaaggga tgcccttcgt
    16561 caaaaaactc tctagtcccc actaaacaat tggcttctgt attcgttaat tcagaaaaac
    16621 gataccaaac ttcttcatct gagtacaggg agcagatagt ctctagataa tcttttacaa
    16681 attgctgaca acttgctaat atgatgtttt gattaagctc tctcaacaaa tattccccac
    16741 gaatcatgcc gacgtggtga aatagttgag gataaatttt ttcaagaatt ttttcgccac
    16801 taagggcaag ttgatttctc atcattcacc ttccaattca tgtaagaagt cttgtccagt
    16861 tctggaaatc ctaataattc agacttaacc ttcaagacta atggcgatgc attttcttct
    16921 gtaatctctt gaatatccat ccaaatatat ccaagtgaat cattcgcacc atcagacaca
    16981 gcttccgaaa tcgtaacttg aggtgcactc tcattcattt caacatcata caaggccatg
    17041 acatggtgaa ccataaaatt tgttaactct tctctgacga aaacatcgta gattcgagga
    17101 ttagagtagc ttctaacagt aaatcccgtc tcttccataa cttctctagt cagcgtttcc
    17161 gtcagtcctt caccaagttg ctgactgcct ccaggtagat cataccgatg ttgataaggg
    17221 cctctcgttt tttcaatgca aagtaacttt ccattttcaa agcaaacaca gtagacccca
    17281 aagtgatttt tgatttccat ccaactcctc ctacttcaaa gaccagccac catctattgt
    17341 caagatttgt ccttgcatgg cgctcgcttt tccacttgct aaaaaaagac taagctctgc
    17401 tatttcctct ggttcaatcc agcgcttgat tggtgtttca ctagccaccc agtcagctaa
    17461 gccacttggt tcaaaatccg cagcggtcat agctgtcttg actgctcctg gagcgatacc
    17521 aaagacctga atcccagctt cagcatagtc tagagccaac tgcttggtga agccagccaa
    17581 ggcatgcttg gatgaagtat aggcgtgacc acctccacct gctaggctag aagcaatgga
    17641 acacatattg atgatgattc cctttttatt ttccagcatt tgtgtcaaat aataccgagt
    17701 caactctact ggaataatgt agttgatttc aaaaatctct tgaatgtcct gcgccgtttg
    17761 ttccaacagt ggtttgtaat catccaaaac tccagcagta ttacacaaaa catccacctg
    17821 agggcaccag tcaaaaatag gttccaagtc caaggtcaaa tctctctgta aaaagtgaaa
    17881 atcaccctct aagagtggct tttcaccttg gtcaactcca taaacttgat agcccttctc
    17941 taaaaagagg cgagcttgag ccaatccgat ccctgaactc actcctgtaa tgagtacacg
    18001 tttagtcatg cacttctacc caatccgttg ccaaaacatc acaaactgtc gggctccaca
    18061 tggaaaaacc ttctccttcg ccagaaacgt tgattaggaa ataaggtgtc atttcaagtg
    18121 caagcccatt ttgctcgatg gtatcaaaga gttggacata gttttccgca cctccccaac
    18181 cagttcgtac atattttctc ttagccttta acccaggcag gatctcttca aatgtcatgt
    18241 ttttctcctt taattctaca ttcttcattt aattatagca aaaaaccgct ttatacggct
    18301 ttttgaatgt gagttattca aacctgctac tacttacggc aaattattcc ctgcagcaag
    18361 ataaatttca taccattctt ttcttgttaa gctaaagttt gccgctcggc taacttctct
    18421 caagtgctta ggatttgttg tacctacgac tgcctgcatt tttgctggat aacgcaatat
    18481 ccaagaaatg gcaatagttg aagaggttac tccatattta atagctaaac gatcaagtac
    18541 ttgatttaaa gcttgaaatt tctcatttcc aacaaaattc cctttaaaat acccgaattg
    18601 taagacagac catgcttgaa tgaccacatc gtgtaattgg caatattcaa aaatgctgcc
    18661 atctcgcata gctgcttgac tatcttccat attaacatga aaagctgatt caaatcctgg
    18721 agtaaaagcc gcactcaatt gtagctgatt aacagctaac ggctgcttga catctttttt
    18781 aagcaactcc atcatcatag gattttgatt agaaactcca aaatctcgaa ctttaccttg
    18841 tttataaagg agattaaagg cttctgctac ttggtcagat tccatcaaag catctggtcg
    18901 atgaaggagc aagctatcta gatgatcaat cttcaatctt tgcaaaatac cgtctactga
    18961 ttttataata tagtccttag aaaaatcaaa ataggtaaat tcttcaatgc gaatgccaca
    19021 tttggactga atccacatct tttctcttaa atctggacga ttttttagga caagacctaa
    19081 cagttcttca caacgaccac gaccataaat atcagccaaa tcgaaggcat tgattccaac
    19141 agaaagtgct gtttctacaa gctcttcaac ttcttttaca gacttatctt ttattctcat
    19201 cattccgaga acaatttctg ataattcttt gtcatcttga ccaagagtta tgtatctcat
    19261 caaatttttc tcctttaatt tctaacattc ttcccttcat tataacaaaa aaccgctttg
    19321 caacggcttt ttgactatac ttcactccat tttatcttct taaacccacg gaacaagaca
    19381 aagattccaa taaagaggac agctaaagga ataacttttg taaggaaaac atttgaaatt
    19441 cccatccact cataataacg gagcagagaa cccaccacaa gatgggcaat aatcatactg
    19501 acaaatggac gaaagaccgc ttctttccaa ttccaaatac cgataactag cgaaatcgta
    19561 aagacagaca aactatccca gggagccgga atataaaagg ctccttcttg tatgaagctt
    19621 gccattccta catatcctaa aacaactaga agaactatag tcccaacaac aatgtaagtg
    19681 ccaattttca ttttaggaga atcttggact aaacttcttc gtaaaattgt ggccacaagt
    19741 ccaaatccaa tcagaaaaat aagaagttgc cctaaaaatg tgagcaaatt gactgttaag
    19801 agaggacctt tagaaaaatc acttagtagt tgataataac gtaataccgc caggacaaga
    19861 attggcgtca aaagggactc tttgatagaa ctgcgaggtg ctcccttgag aatctctttc
    19921 attatttttt taggattctt acctagataa tcctctgcac tcatgccatc tcgttctgct
    19981 tctgagaaat ctagcatcat caaatagatc tgctctctga gatagtcttc atcatagaga
    20041 aatccagcaa gattaaaact ttcccacaac tcctcaaaat acttttgatt ctcctcagaa
    20101 aactcatgta gcaaagcgct tgtttcttcg taatacttca ttttcttcat ggtttaaccc
    20161 ccattcttaa tcccttctac tttttgactc aaatcgtccc attgttgcca aaagactgag
    20221 acacgctctt ctccttcttt cattaatgaa aaatacttcc gatctggacc atctggcgac
    20281 gggcgcatgt cgcctcttat ccattgattt ttttctaact tttgcaacaa aggataaata
    20341 gttcctggaa cgatagtatc aaatccagcc tctcgcaaag tctgaaccaa ctcataacca
    20401 taccgctctt tttgaccaat catatccaag acacaacctt caagaacacc ttttaatagc
    20461 tgagtttctt tcatcacttc tcccttctaa tctattttgt aatacctact agtaacttca
    20521 cctatagtat atcacttcta cactagtttg taaagcataa tagttaatac tcttcgaaaa
    20581 tctcttcaaa ccacgtcagc gtcgccctac cgtatgtatg gttactgact tcgtcagttt
    20641 catctacaac ctcaaaactg tgttttgagc tgacttcgtc agtttcatct acaacctcaa
    20701 aactgtgttt tgagcaacct gcggctagct tcctagtttg ctctttgatt ttcattgagt
    20761 ataaataaaa aaacagaact agcctgaact agtcctgtct acttttaccc aatcacactt
    20821 ccatttggta cagctggatc aactgtgaga agggttaatt tgccatcatg ttcagctgag
    20881 agaatcatac cctggctgac atattttttc atcattttac gtggtttgag gttagcaatg
    20941 atttgaactt tcttgccgac caattcttgt tcatttggat agtattttgc aattcctgaa
    21001 agaatctgac gatcttctcc atcaccagca tccaagcgga attgaagcaa cttatctgaa
    21061 ccttctactt tagacacttc tttgacttct gcgacacgga tttcaacctt gtcaaagtct
    21121 tcaaacttga tttcatcctt gtttagtttg agctcaactt cgtccggatt ccattctttt
    21181 tcgactgctg gtttattgcc ttccatttgt tccttgatat aggcgatttc ttcttccata
    21241 tttagacgtg gaaagatagg tgttcctttg gcaactacag tcacatctgc tgggaagtca
    21301 gccaaactca agttttcaag actagaaact tcttccaaac caagttgagt caaaactgca
    21361 cgactagttt ccatcataaa tggttcaatc aagtgagcaa ctacacgaat gctggctgcc
    21421 aagtggctca tgacacttgc caattggtca cgaagagctt catccttggc caagacccat
    21481 ggtgcggtct catcgatgta tttattggta cgagagatca gagtccagac tgcttcaagc
    21541 gcacgtggat agtcaactgc ttccatgtgt gtatggaagt ctgcgattga ttgttctgca
    21601 acctcagcaa gaacatgatc aaattcagtc acaccttcta cataggcagg gatttgtcca
    21661 tcaaagtact tattaatcat ggaaaccgta cggttaagga ggttcccaag gtcattagcc
    21721 aattcatagt tgatacggcc gacatagtct tcaggagtaa aggttccgtc tgaaccaact
    21781 ggaaggttac gcatgaggta gtaacgaagt ggatctagtc cataacgctc taccaacatt
    21841 tcagggtaaa cgacattccc ttttgactta gacatttttc cgtctttcat gacaaaccaa
    21901 ccatgggcaa tcaaacgatc aggtaattta acatccaaca tcataagaag gattggccag
    21961 tagatagagt ggaagcgaag gatatctttt cctaccatat ggaagactgt tccattccag
    22021 aacttgtcaa agttaccatg ttcgtcttga gcgtagccaa gagctgtcgc atagttaaga
    22081 agggcatcaa tccaaacgta gacaacgtgt tttggatttg atgggacagg cactccccat
    22141 gtaaaggttg tacgagatac cgccaaatct tccaaacctg gctcgatgaa gttgcgtagc
    22201 atttcattaa ggcgaccatc tggcgtgata aattcaggat gagctttgaa aaattcgacc
    22261 aaacggtctt ggtatttgct aaggcgaagg aagtatgatt cttcagaaac ccattcaacc
    22321 tcatgacctg atggagcaat accaccagtc acatttccag cttcatcacg gaaaacttct
    22381 gccagctggc tttctgtaaa gaattcttcg tctgatactg aataccaacc agagtattca
    22441 cccaagtaga tatcatcttg agcaagtaag cgttcaaaga cctgtgcgac aactttttca
    22501 tggtagtcat cagttgtacg gataaattta tcgtatgaga tatctagtaa ttgccagagt
    22561 tctttaactc caaccgccat tccatcaaca taggcttgag gtgtaatacc agcttcttcc
    22621 gctttctgct ggattttctg accatgttca tcaagacctg tcagataaaa tacatcgtag
    22681 cccatcaggc gtttgtaacg tgctaggaca tcacatgcga tagttgtgta ggcagaaccg
    22741 atatgaagtt tcccagatgg atagtaaatc ggcgttgtaa tataaaaatt tttttcagac
    22801 ataatttttc ctttccaggc aaatgaaacc tgtttttcta acacttcatt atatcacatt
    22861 tttaatgaat ttcgataggg aaatccatac aaaaacaaga tagacgagtg tccatcttgt
    22921 tgatctcatt cataacgaag ggcttcaatt ggatcaagtt tcgatgcctt gttggctggc
    22981 aagactccaa aaatcatacc aacactagcc gaaactgcaa gactaaatag ggcaactggg
    23041 attgatactc caacttctat accttctatt aaaccttgca gtaacaaacc tgctaaggca
    23101 gttaaaccac ttgcaattgt caagccaatt aagccaccta acaaggtcaa aatcatggat
    23161 tcaatcaaaa actgaattaa aatattggca cgtgttgcgc ccaaagcctt acgaagacca
    23221 atctcacgag tgcgctctgt caccgaaacc agcatgatgt tcatgacacc agttcctcca
    23281 acaaagagag aaatccctgc gatggaacta ataatcgtcg tcataaaact aaacgattgt
    23341 tgaatttctg caaatacaac ggactcatct gccacctggt attctccctg ttgtaagcct
    23401 gcaagctctg tcatttttcg tgccagttct ggacccagag ttggggttaa actggtatca
    23461 ttcactcgaa agacaatact agctatttca tctatattaa aattcgcagc aagggagata
    23521 ttggtagtaa taggcaagcc accaaaccca tatatttttg accttttagc ctccggacta
    23581 gtataaaccc caatgacccg gtaactaaat ccattgactt ctacaacctt gttaatagcc
    23641 tcttgaggag attcaaataa actaatggac aattcctcat ctagcaaaat aacacttgca
    23701 aactctttga aatcttgctc tctcagacta cgacctgcaa taatttcatt cttaacagcg
    23761 tccatgtaag ttctgtttcc acctgtcaaa ttagcattct caaccttttt atcttgatag
    23821 gtcaagatgg cattcgttga attggttaca tagtaactat ccactccctt cagtttagct
    23881 gcctcttgga cccaggattc ttgcggtttt ggcggttcaa caggaatttc ctcttccttt
    23941 ccagaaaccg taaaagatga ttgtttctga gtaaaagaac catctttact ttttttagga
    24001 gagaaaaaga cgctaatatt tttctgagat ttagtcatat ctttattgac ttgacgagat
    24061 agggaatcac ccaaagccat aatcacaaca actgatgaaa caccgataat aatcccaatc
    24121 atagtaagca aagaacgcat cttgtgagcc atgatagatg aaaaggcaaa tttcagattc
    24181 tgcatcttag ttttcctcct ttcctaactg agcactgtca gacgaaatga ccccatcccg
    24241 aatgacaatc tgacgtttag cataggcagc aatctcaggc tcatgcgtta ccatgataat
    24301 ggtttttcct tctttattca aatcaaccaa taattgcata atttggttac ctgttttggt
    24361 atccaaggct cctgtcggtt catccgctag gataatagaa ggattgttta ccaaggcacg
    24421 cgcaatggct acacgttgct tttgaccacc agataattct gaaggtaaat ggtgactacg
    24481 ttctgtcaat tcaaccttgt ctaaatattc ctcagccaac ttgcgacgtt ttgaagacga
    24541 aactcctgcg taaatcaagg gcaattctac attttgcaga gcattgagct tcgatagaag
    24601 aaagaactgc tgaaagacaa aaccgatttg ttggttacgg accttagcca gttgtttttc
    24661 accaagccca gccacttctt gaccttcaag ataatattct ccactggttg gtgtatccaa
    24721 catgccaatc gtattcatca gagtggactt accagaccca gatggtccca tgatggctac
    24781 aaattcaccc tcattcactt ctagattgat atttttgaga acctgcagtt cttggtcacc
    24841 attacggtag cttctgaaga tattttttag actaattagt tgcttcatca gccttcacct
    24901 cttttccttc ttccaaggaa gatgttggat tactgatgac cttagcacca ttcgttaaac
    24961 cagaagtgat ttcttgattt tctgcgtcag catttcccaa tgaaacctca acttttttag
    25021 ccttttgttg ttcatccaca atccagacat aatttttact atcatccatt actagactgc
    25081 taacaggaac aagaatagcc ttagttttgc ttttaacctc aatgttgaca gaaaaaccct
    25141 gtttcaaatc accaacctcg cctgtcacat caatagtata agggtattta gaacctgtat
    25201 tattcccggc tgctggacta gctgcttcac cattgttttt aggatagtca gaaatatagc
    25261 ttaatttccc agtccatttt ttatcaggat acactttaga agtaaagctt acttcttgac
    25321 ctacagaaag gttggctaga ttgtactcag acaattctcc cttgacttgt aaattttcat
    25381 tgctgacaat atgaaccata acttggctcg cccctgttgg agatttagaa acattgctat
    25441 tgacttcgac tacagttccc tctagggtac tgagaacagt tgttgcatcc aattgacttt
    25501 gagccttgct taattgcgcc gcagcatctg cacgcgcatc acgggcatca cccaattgag
    25561 catcaataga agcaacagaa tttccagcca ctggagttgg gctttgcacc gttgcatctt
    25621 ctcctcctac tggcgctggt aactgtggag ccggagctga agcggcttca tttcgtgctt
    25681 gattgagttc attgatatga caatctgccc tagctactgc tcgactagct gaatcatagg
    25741 ccgcctgcgc ttctgaacta ctgtacttga ctaaagcctg cccttcgctg accttatcgc
    25801 ccacagaaac aaggatttca tctaaatcac ccttactagc atcaaaataa acatattgtt
    25861 cattttttgc tgttactgtc cctgacaata aaacagagga ggccacgctt ccttccttgg
    25921 caacaacaag atgagtaggc tcatctttta gagcagtctg agaaggttgt ctaaagagta
    25981 aaatcccccc ggcacccaat acaactacac tcgcagcacc gattgctgca tacagttgcc
    26041 actttttagc tttaccattc tttttcttca taatgaaact ccttttcttt tttacaatac
    26101 tttgctatta taccaaattt ccctccagca aacaatgcag ttcagatgga aataatggca
    26161 gttcaggatt aaacaatcgt tcggaatttt gcttttctat tgtactagat atataataca
    26221 ccatcttgac ttatttggca agcttttttg caattttttg tgcgtagcag atttttgcaa
    26281 tcaaatcaaa aaaagaataa aatataacta tcaaaaaaga cgctctacta tttaaaagat
    26341 tacaaaggag ttttcaatga gagaatatga tatcattgct atcggtggag gtagcggagg
    26401 aatcgctacc atgaaccgtg ctggtgaaca tggagccaaa gcagccgtta ttgaggaaaa
    26461 gaaattaggt ggaacttgtg tcaacgtcgg ttgtgttcct aaaaaaatca tgtggtacgg
    26521 ggcacaaatc gctgagactt tccatcaatt tggagaagac tacggcttta agactactga
    26581 tcttaacttt gactttgcaa ccctacgtcg caatcgtgaa tcctacattg atcgcgctcg
    26641 ttcttcttat gatggtagtt ttaaacgcaa cggtgtagac ttgattgaag gtcatgctga
    26701 atttgtagat tctcatactg taagcgtaaa tggtgaactg attcgtgcta aacatatcgt
    26761 gattgctaca ggtgcccatc caagtattcc taatattcct ggtgctgagc taggtggctc
    26821 ttctgatgat gtatttgcct gggaagaact tccagagtca attgccattc taggcgctgg
    26881 ttatatcgcc gttgaattag ctggcgtact ccacactttt ggtgtcaaga cagatctctt
    26941 tgttcgccgc gatcgtcctt tacgtggttt tgattcctac atcgttgaag gtttggtcaa
    27001 ggaaatggaa agaacaaact taccacttca cactcacaaa gtccctgtca agttagaaaa
    27061 aactactgac ggcattacca ttcatttcga agatggtact agtcacacag ctagccaagt
    27121 tatctgggct acaggtcgcc gtccaaacgt taagggcttg caacttgaaa aagctggagt
    27181 gactctgaac gaacgtggct ttatccaagt ggatgaatac caaaatactg ttgttgaggg
    27241 aatctatgct ctaggtgatg taacgggcga gaaagaactg actccagttg caatcaaggc
    27301 cggacgtacc ctatctgaac gtctctttaa cggaaaaact actgcaaaaa tggattactc
    27361 aactattcca actgttgtct tttcacaccc tgctatcgga actgttggtt tgacagaaga
    27421 gcaagctatt aaagaatacg gtcaagacca aatcaaggtt tataaatcaa gctttgcatc
    27481 tatgtactct gcttgcactc gcaaccgtca agaatcccgt ttcaaactca taacagctgg
    27541 ttcagaagaa aaagttgtcg gacttcatgg aattggctac ggcgttgatg aaatgattca
    27601 gggatttgcc gttgctatca aaatgggagc aaccaaggct gactttgatg caactgtggc
    27661 aattcaccca actgcatctg aagaatttgt aaccatgcgt taatcgaaac aaaagaagct
    27721 gatataaata tgtctgcttc ttttttaatg attttcgaat caacaaaaaa attatccaac
    27781 ttgactagtc acgaaaatcg gctgttacag tttttgttac cttgttttta aaaaatggtt
    27841 gacatcgact cctatatgag tataatagtt actatacatt agaaaagagg ttaactattt
    27901 tgaaaaaagc tcacgtttat gctatccctg ctattggggc tgctctcatt gctgctttgg
    27961 cacaaatcag tcttccaatt ggacctgttc ccttcactct gcaaaacttt gcaattggct
    28021 tgattgctac tgtctttaga ccgagagagg ctgtactttc tgctggactc tatcttcttc
    28081 taggtgctat cggtcttcct gtctttgcag gaggtggagc tggttttcag gctttagttg
    28141 gccctactgc aggctatctt tggttttatc tcgtttactc tggacttact tcctctctaa
    28201 ccaacagcaa gagtggtgtt gttaagattt ttcttgcaaa cctcttgggt gatgcccttg
    28261 tctttgtcgg cgggattctc agcttgcatt tcctagctgg aatggcattt gaaaaagctc
    28321 ttgctgtggg ggttcttccc tttatcattc cagaccttgg caaacttctg gctattagtt
    28381 ttattagccg tcccctactt caacgcctta aaaatcaggc ttactttact aactaaaaaa
    28441 ggatatcgag ttatcatgac tcaatatcct tttcttttat tttgaaaact tatactcaat
    28501 gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgc tcaaaacact gttttgaggt
    28561 tgtggatgaa actgacgaag tcagtaacca tacatacggc aaggcaaagc tgacgcggtt
    28621 tgaagagatt ttcgaagagt attagttgcc tttaaaggca tccaccatcg tttgaaattc
    28681 ttcatttgag agagtaatcc ctttgcccat tttagtatgg tctggactcc aagcacgaat
    28741 atcaaacttt gcaggggcac cattaaagct cacacggtta atttccttgg tccaaccttt
    28801 ttcgttttca gaaagagtca acaagtgctc ttcgatttca aatgtaaatt ctgccatttt
    28861 cttctccttt tttagtttca ttagtttatt cgtaaaatct tgtagatttt aggaaaattt
    28921 tatataatat tgatataaaa gaagggaggc caatatgaga cataaattcc agcaagttct
    28981 aaataaaata catgattttt taaatggata tgaccaacct gaccagactg aaaccaactc
    29041 ccttacagcc actattgaag aggctatcca gaaacaaacc gctgttcacc ttatcttgtc
    29101 tgagacaagc tttacaggtg acatcatcaa atatgatcag caaggccagc aaattatcgt
    29161 gaaaaatttt tccaaaaatg tgagccggat tatccgtata agcgatattc aacgcctgcg
    29221 atttgtcccc tcaactgtcc aaacagccca aaaaaataga tttaagaaag agtgagatgt
    29281 agttgcttca tcccactctt tttcttagcg aatttgttca aaatgtaaat gaactgcaat
    29341 atgatctcca taaccacttc tttccaagtc acgttgtaaa cgataggaaa tgtagtgttc
    29401 tgcaatggta atgtaacctg cgcccaataa acgatgttca accatagatt gaatcatact
    29461 gatagtcgca cgttccacct tggcttcttg taaatccaaa actaccttct tagtgacttg
    29521 agcaagattt tgacgcaaat catctgtcaa aacataaaca gtttgggctg ccttcaagat
    29581 ggcttggtaa atcttatctg gattaaattc agcaatttcg ccattacgtt tgattacttg
    29641 cataggtttc tcctttattc tttgttttct ttgatttctg ccagcatttt ttcttcttct
    29701 actgtcagtt gataatgttc aagtaaatct ggtctgcgct cgtaggtttt ctttaaactc
    29761 tcgtacaatc gccactgacg aatcttttca tggtggccac tcatcaatac atctggcacg
    29821 accatgcctc gataatcata gggacgtgtg tactgaggat attctaaaag acctgaagaa
    29881 aaactatcat cttggtggct agactccttg ccaatcactt ctggaatcag gcgaactgta
    29941 gcatcaatca tggtcatagc tgccaattct ccaccagtga ggacatagtc gcctagggaa
    30001 atctcatctg ttaccaaggt cttaatgcgc tcatcataac cctcatagtg cccacagata
    30061 aagattagct cttcctcttg agccaaatct tcagcataag cctgatcaaa ctgctttcca
    30121 gcaggatcga ggagaataac gcgcggattt ttcttttcaa tagcatcaaa ggaatcgaaa
    30181 ataggttgtg ctctgagcaa cataccctga ccgcctccgt agggctcatc atctacatga
    30241 cgggcctttt cagcattttc tcgaaaatta tgatactgga tatccaagag ccctttttct
    30301 cgagcctttc caacgattga gtgctccagt ggagaaaaca tctctggaaa gagggttaaa
    30361 atatcaatct tcatcgtcta acccttctaa gatttccaca tcgacccgtt tatttggaat
    30421 atcaacattg agaaccactg gtgggatata aggtaaaagc aaatcacgtt tgccttttcg
    30481 tttgaccacc cagacatcat tagcacctgg ttgcaggatt tccttgatgg ttccaaccaa
    30541 gctatcaccc tcatagactt ccaaaccgat aatctcgtga tagtaaaatt caccatcgtc
    30601 taggtcattc aaatcttcct cagcgacctt gagactatat cccttgtact tttcgatagt
    30661 attgatatgg tacatatctt tgaatttaat aatgtcaaag ttcttctgtt tacggtggct
    30721 agcgatggtc actgtttgga caaactgatc tttttcatca aacaaagcca gctcagctcc
    30781 ttttttaaac cgttcttctg caaaatccgt cacagacaag actcgcatct caccctgtaa
    30841 tccctgcgta ttaacgattt tcccaacatt aaagtagttc atcttgtctc ctgtaatctc
    30901 cttttttcca tcttattctg acaattctcg aataatagcc gcaatttttt ccgattctga
    30961 ccattgtaaa taatggtgat tccctcctaa aatgagttta gtattggaag tccaatattc
    31021 tgattctctg tactcttttt ctctataagg ctgacaaaaa acaaatacag gaatatgagc
    31081 ttctatagat acatcctcaa aatcttcctc agtaatctct ccagatatct gaaattctgg
    31141 atcttgattt tccaactcta agcctttttc ttgcatcaat tcccagattt ttttattcgt
    31201 ttcaggacta aatgttgctt gagttaagtt cttaaaataa agttcaggac cacactcgtc
    31261 aatcagcctc atctgctctt ccatttctgg ataaggattt tctgaaaaat cagcaaacat
    31321 gactttttta gttgtcggtt caattgctac taaagtctga cacttaattg gtttctcgag
    31381 taatttgcaa gctaaaattc cactccaact atgtgcacaa agtatatatt cagaaatccc
    31441 taattcttca agtacttcat aaaccacatc tgcaagatta tctagatttt ttccagcttg
    31501 gtcatgaatc ggactcctac ctgtgttcgg aaaatcaatt gtcaaataac caattgtagg
    31561 aggaggtttt tcaagtataa gtgaaaaatt ttcataactt ggtagcaaac ctgctccgtt
    31621 tagacaaact agcactttct tttgcttttg ataagcaaca gagagactac caatttctgt
    31681 agatacttca aacctcttca taaagaaatc cactcattct atataatgaa ttattaaaaa
    31741 tccttatcct ttattttatc acgttccaag gattttctca agttggagga aggggacaac
    31801 atctctactt tcccttcaaa aatccttcca aatcatgttt atgttggtaa ttaacggctg
    31861 cggttttgtc tttctcaaag acagtcttgg taaggtcaat atgattaata gctacgattg
    31921 cgacggtgta gtaaatgata tcagccagtt ctctggcaag ttcctcgttc gaatcctatc
    31981 ccttcttttc gaccagagcg cctattcaaa acctcggcta cttctccgac ttcctccact
    32041 aacttcataa agagaccttc atcagtccga gactgctgtt aatgttcgag taagtagtct
    32101 tggaattgcc taaacgttca atcttttata gtatattgaa actagaatag tacaccttta
    32161 cttctaaaac attgttagaa atcgatttga ctgtcctgat cgatttgtcc tgttattatt
    32221 tcattttact atatcttcta ttccacacaa aaaagcgaga catccgtccc gcccttctta
    32281 tttttcgtca ataacgattc ttactttttt gtattcagtt gggacagagt agacaatcgt
    32341 tcttatcgca gaaatagtgc gacccttacg accgattaca cgacccacat cgctttgatc
    32401 aagattcaaa tgatattcca aaaattctgg tgtatcttca atcttgatag ttaaggcatc
    32461 tggttgtgaa attaagggtt tcacaatcgc aataatgaga ttttcaatcg tatccatctg
    32521 tcaacctact ttaaacttat tttgagaatt tagaatcgtg gaattttttc aatacgcctt
    32581 cttttgaaag gatgttacgt actgtgtctg aaggttgagc tccattagcc aaccatgcaa
    32641 gaacgcggtc ttctttcaaa gttacttggt tttcagcaac aagtgggttg taagttccaa
    32701 ctgtttcgat gaaacgtccg tcacgtggtg aacgtgaatc tgctacgttg atacggtaga
    32761 aaggtttttt cttagaaccc atacgagtca aacggatttt aactgccatt tttaaagtct
    32821 catttcttta attttttatt tcggtgaaat agctgagcta tttagcacat gttctattat
    32881 agcagatttc tggcaggtgt caagaaaaaa acttgacaga ctataaattt cttaaagcat
    32941 ttaaaaattt gttagaatag gaaataaatg tttgaaagat gctatccgtg aatactattt
    33001 tagaaaaatt ttataaagaa caccaagtca aacccttcat atcgcctgaa cgagatttgg
    33061 atacttggct tctaagtccc aagcccgttc ccaagcgaaa tatggactta ttagtagatg
    33121 attcactagc aggagatatc attttactat ggagaatcca gtttggtact tttaccactg
    33181 aaacatgatt ttaaaaactc ccattcgaat atgattcaaa attcgcttag aatcaatgtt
    33241 ttcccacttt ttcaaaaaat aaattttcat ctaatttaaa taactttcaa ttaagtgtga
    33301 attttgctta aaatcattaa aaaacacccc atggtgtgaa ccatgaagtg ttgtaaatgc
    33361 tgtgttgtag ctagttctta gcgtttagag aactggctag ctttacgagc tttcttaaga
    33421 tctggtttcc aaaatacttt gagtacactt atggtattga tgcccctaaa catctaaaaa
    33481 ctctggtaga aaaagggtat gctcttgtcg aaacttcttt tgactcactt gaccatctaa
    33541 atactaccat gaaaaagaat atcctgaaga agggagttac tggactttct aaaatgaaat
    33601 cttgcgacct ggatcaagcc cttcatgagc atttttcaga agaagaatta gctggtcact
    33661 ttcatgtcct tctatggact tttttacaat ggcattgcta tcacacccaa tacctatcta
    33721 agcgcctggt tcgtaaactt tattgcagct cttcctctaa atttcctaat tgttgaacca
    33781 attgcccgtt ttatactaag ttcttttcag aaaccattta ctggggaaga agttgaagat
    33841 tttcaagatg atgatgaaat cccaactatt atctaagcca gttctgtaaa ctactaatat
    33901 ttgaaatcca cttcctttta gggtacaatg gtataaatga atttttgaga ggatcagaat
    33961 gaaaaaacta gcaacccttc ttttactgtc tactgtagcc ctagctgggt gtagcagcgt
    34021 ccaacgcagt ctgcgtggtg atgattatgt tgattccagt cttgctgctg aagaaagttc
    34081 caaagtagct gcccaatctg ccaaggagtt aaacgatgct ttaacaaacg aaaacgccaa
    34141 tttcccacaa ctatctaagg aagttgctga agatgaagcc gaagtgattc tccacacaag
    34201 ccaaggtgat attcgcatta aactcttccc taaactcgct cctctagcgg ttgaaaattt
    34261 cctcactcat gccaaagaag gctactataa cggtattacc ttccaccgtg tcatcgatgg
    34321 ctttatggtc caaactggag atccaaaagg ggacggtaca ggtggtcagt ccatctggca
    34381 tgacaaggat aagactaaag acaaaggaac tggtttcaag aacgagatta ctccttattt
    34441 gtataacatc cgcggtgctc ttgctatggc taatactggt caaccaaaca ccaatggcag
    34501 ccagttcttc atcaaccaaa actctacaga tacctcttct aaactcccta caagcaagta
    34561 tccacagaaa attatcgaag cctacaaaga aggtggaaac cctagtctag atggcaaaca
    34621 cccagtcttt ggtcaagtga ttggcggtat ggatgttgtg gataagattg ctaaagccga
    34681 aaaagatgaa aaagacaagc caactactgc tatcacaatc gacagcatcg aagtggtgaa
    34741 agactacgat tttaaatctt aaaaaccaaa aaaaatacag tatccacatt cggtactgta
    34801 tttcttttac tctcattctt aagttaaatt attaaaatcc catatttggt ctatccagcc
    34861 ttcataaaag tctagctcgt ggcagaccat aaggatagat cccctatatt ctttgagagc
    34921 gcgtttgagc tcatcctttg catccacatc caaatggttg gtcggctcgt ccagcactaa
    34981 aacgttgttt tcacgattca tcaagagaca gaaacgaacc ttggcttgct ctccccctga
    35041 taatacttga atctggcttt caatatgttt ggttgtcaaa ccacaacggg caagggctgc
    35101 acggacttct gcttgattaa gggcaggaaa ggcattccag acagcttcaa gaggagtttg
    35161 gcgattaccg ccttctactt cctgttcaaa ataaccaagt tctaaataat ctccacgctc
    35221 cacttcccca gcgattggcg agataatgcc caagagactc ttcaagagag ttgtttttcc
    35281 aataccatta gcaccaataa tcgcaacctt ttgattgcgt tcgaaggtaa gatttaaagg
    35341 cttagtaaga ggacggtcgt aaccaatttg caagttcttg gcttggaaga taaagcgccc
    35401 tggtgtacga gctggtttga aatcaaagga tggttttggt ttctcacttt ggagttcgat
    35461 aatatccatc ttatccaatt tcttttgacg agacatagcc atattacgag ttgcaacacg
    35521 ggctttatta cgagccacaa agtccttgag gtctgcaatc tctttctgct ggcgttcgta
    35581 ggctgcctct agctgagatt tcttcatagc ataaacttct tggaactggt agtagtcacc
    35641 agagtaacgc gtcagctgtt gattttccac atgatagaca atattaataa cgtcattgag
    35701 gaatggaata tcgtgcgaaa tgagaacaaa ggcattctca tagttttgga gatagcgctt
    35761 gagccaatca atatgctcag catccaagta gttggtcggc tcgtccaaca gcaagatatc
    35821 aggcttttca aggagaagtt ttgccaaaag caccttggtt ctttgcccac ctgacaaaga
    35881 agttacatcc gtatccatgc caaagtccat aacaccaaga gcacgcgcta cttcgtcaat
    35941 cttagcatcc aaggtataga aatcacgact ctccagacgg tcttgaagtt ctcctacttc
    36001 ttccatgaga gcatcaacat ccgcgccgtc ttcagccatt ttcatataga ggtcattgat
    36061 acgagcttca gctttgaaaa gctcatcaaa agccgtacgg agaacatcac gcaccgactg
    36121 tctttcagca aggacagagt gctgatccaa gtaaccagcc gtcacatatt tggaccactc
    36181 aacctttcct tcatctggca gcattttacc agtcacgata ctcataaagg ttgattttcc
    36241 ttcaccattg gcaccgacca ggccgatatg ttctcccttg aggagacgga aggacacatc
    36301 ttcaaaaatt gcacggtcac caaaaccgtg actcagattt ttaacttcta aaatactcat
    36361 tttaattcct tatcttgttt ttatgtaatc gtttataaag gagccaagcc agatagccac
    36421 ccaaagtgtt ggtccacaaa tcatcaatct caaagacgcg attgaaatca aagaaaaagt
    36481 ctaagattaa ttgcgtacac tcgattccaa gactcacaag aaaactaaaa agaaggacct
    36541 tttttgtttt ccgcaaattt ggaaatagat aaaggagttg gaaaatcaga ggaaaaaaca
    36601 agaagacatt gaggatattt tgtaaaaaaa tccaacataa ttgtccaatg tcactcactt
    36661 cgcccagttt ccagagagaa ttgaaaggag tcaaaagaaa aaccaggcgt ccaagatgct
    36721 ggatacctgg agttcccact cccacggtag attgttcttg aggagtaaag caaaaacaga
    36781 caatgcaaat gctatagaaa atgactcccc agaccaaaat atgattataa gtcttcttca
    36841 tcattaagga tttaccgctg cgactgcctt ctggcggtca cgtttcattg tgttagagcg
    36901 caattgtcca caagctgcgt caatatctgt accatgctct tgacgaacca cacagttgac
    36961 cccttttttc ttaagcgtat catagaaagc caacacgcgc tctttgggac tacggctata
    37021 ttggtcatgc tcactaactg ggttataagg aatcaagttt acataagaca atttcttgat
    37081 gttcttgagc aattcagtca attccaaggc ttgttctaca ccgtcgttga cttcattaag
    37141 catgatatat tcaaaggtta caccacggtt tgttgtctca atgtagtatt caatagcagc
    37201 aaagagtttt tcaatcggaa aggcacggtt aatcttcatg atacttgaac gaagttcatt
    37261 gttaggtgcg tgaagagaca cggcaagatt gacctgaacc ccttcatcag caaagtcacg
    37321 aattttatga gccaaacctg aggttgaaac cgtgatgtga cgagcaccga tagccattcc
    37381 tttatcatca ttgatagtac gaaagaaatt caagacattg ttgtaattat caaagggctc
    37441 accgattccc atgacaacga tatggctgac gcgttcatcc tgaccacgct cagcaaagta
    37501 tttctgaacc agcatgattt gcgctacgat ttcaccgtta ttgaggtcac gttgcttctt
    37561 aatcaaacca gaggcacaga aggtacaacc gatattacag ccgacctgag tggtcacaca
    37621 gacagataaa ccatagtgtt gacgcatgag tacagtctca attaacatac cgtcgggcaa
    37681 ttcaaagaga tatttgactg taccatcagc agactcttgc acaatacgtt gtttcaaggg
    37741 attgaccaca aactggtcat tgagcttagc aatcaaatcc ttggaaaggt tggtcatttc
    37801 ttcaaatgac tgcacacgtt tacggtagag ccattcccag atttgatctg cacggaattt
    37861 cttttctccc tgctccaata cccattcctg catggtttga tgtgccaaac tatgaattga
    37921 gggtttcatt tcttctcctt attctctact cacttctgac gaatgacaaa atgacgttgt
    37981 cccttgtcct ctttctgaga atgctggtct ttctgacgac gtctattttt cttatctgca
    38041 ttcgactttc gtttagtttg agtcggtttc tttccttttc tagaaggtgt ttcttcttcc
    38101 gtcttacgca ttttcttgtc aaatgatgct cgcttagggg cttcattttc taagacaaaa
    38161 taggcacaac cataactaca atactctaaa aggtagtctt gtaaacgact gattttttca
    38221 agtttttctt ctgttcggtc atccttgtaa aaacctcgta ggcgaagctg ttcgttgctc
    38281 cagtccccca cgatataatc aaacttggtt aatacttctg aaaaacgctg attaaaagtc
    38341 gtcacatcaa aggcatcctt gatattttca accaaggaaa aagctatccc ttccgtttcg
    38401 accttgtccc cgtgtaaatg gaactccgga ccaggaaact tgttatagtt gtataattca
    38461 ggtgcaattt cttttcgcat agatatcctt ttttcacgat tacttaatac tttattctac
    38521 cataatttct agcagttagc acgtttctca taaaaatgaa aaaagtctga cgattttgtc
    38581 agaccagaat cttataacct aaaaagagaa gaacaattct tccctccaac tatcattatt
    38641 tagcagctgc gtacaattca tctactttat tccagttgat tactgaaaag aaagctttga
    38701 tgtagtcagg acgcacgttg cggtatttca cgtagtaagc atgttcccaa acgtccaagc
    38761 ccaagattgg ttttttacct tctgagattg gtgtgtcttg gtttgctgtt gaagtcactt
    38821 caagtttccc ttctttgttg acaaccaacc atgcccaacc tgaaccaaaa cgagttgttg
    38881 ctgctgcagt gaaggctgct tggaattctt caaatgaacc aaatgttgca tcgattgctg
    38941 ctgccagttc tgctgaagga gctgttttct cgggagtcat caattcccag aaaagagcgt
    39001 ggttcaagtg tccgccacca ttgttgataa gtgcttgacg gatatcagct gggatagatt
    39061 ctacatcagc aagcaaggct tcaaggtctt caccgatttc agggtgtttt tctaaagctg
    39121 cattggcatt gttgacataa gtttgatggt gtttgtcatg gtgcaaatgc attgtttccg
    39181 catcgatgta tggttccaaa gcgtcgtatg catatggaag ttctggtaag ataatagcca
    39241 tctgtaatac ctctttttct ttctatatga aaatgataac gcaaacattc acttttttca
    39301 agttttttgc ttatagataa ggaaatcact gacatacttt ctaataaaat aagcaaatca
    39361 gcaagaaacc cacgacaaga tagtcctgac gcgggttgta gtttcaacaa atgtcaattg
    39421 acctgactag caatctgtaa gagtgccttt tcaaaaagga aacctttttc ataaagacct
    39481 gtcttaatct gatagtctgt ctcaatcaaa taggaaatag cttgcttcaa aaagctcaaa
    39541 gaaagtcctc tcgaatctct taatgcaaac ttgatttgat aaggatttgg gttacgtccc
    39601 agataactac ctaaactact tgcaatctgc gattctgttt ggccagactc cgccaaaatc
    39661 ttcacctgag taaaagtccg aaattgtccc agcatgactg caatcagttt gatttcatct
    39721 tccccttgca aggtcaagtc tctcaccaaa tcgcgcgcct gatccatctt tttagtcaga
    39781 ataaactgag ttaaatcaaa aatattgtcc tgcaaggtct tgggaattgc gttaacaata
    39841 tcctcttcct caataacaga attcgcctta taggactgta aaaagagaag atttttctgg
    39901 atttcgctaa attgaaaccc cgacttgatg aggagatttt caaaagaatg attggtaaac
    39961 tgcagacctt gtttctgact ccacttttgg aaatactggc gcaattcttg ttcttttact
    40021 tctactgcat cgaagacctt ggcatcacgc ttaagtaatt tgactaaccg tcttttgcta
    40081 tccagctttc cttctgcaaa gattatcaac ttggttgttg gagaagaatt gtcaaggtat
    40141 tcctcaaatg acttaagctc atcatctgtc aaaaagcgtt tcttagcagt cgtgatatcc
    40201 ataaaataat ctaatatcac gattttctca tccgcaaaga aaggaagact gaccaactcc
    40261 agttccacat ccttgtaaac tacttctttc atatcaaagt aggcaaagtt gaggtcagca
    40321 gaatcatacc caatctgttt caacacttga ctcttcatca cttcaaactg accctgatct
    40381 gtccctgtaa ataggctcag gctcggtaaa tttgataaag tcaacttctg actttcttca
    40441 atggctagca tcgtctctcc tttcttcaga tttttcgatt taatttagtc aatatagcgc
    40501 aatttcccac ggaaatcttc taagctctcg tagccttttt ccaccatgat tgctttcagt
    40561 tcattggtaa tgcggtcaaa agcactgacg ccttctttgt gaagggtcgt tcccacctgc
    40621 accatacttg ctccacagag gatgtgttca aaggcatctc gaccagtcag aacgccacct
    40681 gttccgataa tttggatttg aggatttaaa cgttgataaa aggcgtgaac attggctaga
    40741 gcagtcggtt tgatgtattc tccaccaatt ccaccaaaac cattcttagg ccgaataacg
    40801 acagattcgt cttctatata gaggccgttt ccgatagagt taacgcagtt gacaaacttg
    40861 agcggatatt tgttgaaaat agctgccgct tgatcaaagt gaacaatatc aaaataaggt
    40921 ggcaatttaa ttccaagagg tttggtgaag taagcaaaca cttctgccaa aatccggtct
    40981 gttgtctcaa aatcataggc aatctgaggt ttacctggaa catttggaca ggaaagattt
    41041 agctcagtca gaccacgaaa atcactctct tggacttttt tcaaaatagt atgggtttcc
    41101 tctggagaca tgccgaccag agataagaag aaagttcggt tcgactcttt ttcctgcaaa
    41161 tccaaaagat aatccaaata atagtctaag ccattatttg gcaagcccat agagttgatg
    41221 gaaccaagtg gaacatcttg gtagcgtggc tcaggattcc cctgacggaa gtccaaggtc
    41281 gctgtcttag taacaaaggt tcctgccgct gagtttttga cctcttctaa ctcctctatc
    41341 gtcatacaag ccacacctgc tgcattcatc aagcaattgt caaactcaaa accagcaatt
    41401 tgtgttttcg ttgataccat gattctaatc ctccagattt cttttattgc taatattata
    41461 ccataatttt aattctaggt ttactagatt ctgtggttct atctatttat tcggaaaaga
    41521 aagtctagta cagaagtcat gctatggaaa cagctagtgt attagaagtt tgaacaaggc
    41581 ttgggtaaca gacttttgaa tgaagtccaa gcaacaagga gaaatctcaa ccatgagtcc
    41641 attccatgac tctgttgctc tagaaaacct tgcaatagtc aagtgataac gaataaaact
    41701 ttggattgaa atatccccaa gattatctat tacgaggaga aatcttaacc tttatcattt
    41761 ttaaagaaat atagtaaaat gaaacaagaa caggagaaat cgatcaggac agtcaaattg
    41821 attgctaaca atgttttaga agttgaggtg tactattcta gtttcaatct actatacatg
    41881 taaatctcta ctttcgcaaa atggattttc agaacagtat cccagtatag ttaaaaacta
    41941 taaaaaagag aggacgttcc cctcttaaaa tcttatttta ctgcttcttt caaagcatct
    42001 accttatcca aacgttccca tggaagatca atatctgtac gtcccatgtg accgtaagcc
    42061 gatgtttgac ggtaaattgg acgcttgagg tccagcattt ggataatccc tgcagggcga
    42121 aggtcaaaga tttgacgagc cgctttttca agttgacttt cagctactgt tcctgtaccg
    42181 aaagtatcga tacgaacaga aacaggttgc gcaacaccga tagcataggc caactgcact
    42241 tctgccttct tagcaaggcc tgctgcaacg atattcttgg caatatagcg agccgcataa
    42301 gaggctgaac gatccacctt agtcgcatct ttaccagaga aggcaccacc accatgacga
    42361 gagtagccac cataagtatc tacaataatc ttacgaccag tcaaacctga gtccccttga
    42421 ggaccaccga ttacaaaacg acctgtcgga ttgataaaga attttgtctt atcatcaaga
    42481 taagaagatg gaataacttc tttgatgacc ttgtcaatca catcttgatg gatttgttca
    42541 ttagtggcct ctggatcatg ctgagtagaa ataacgactg tatctacacg taccggacgg
    42601 tcattttcat cgtactcaac tgtaacttgt gattttgcat ctggacggag atagctaatt
    42661 tctccagact tacgaagttc tgccagacga cgaaccaatt tatgactgag tgcaattggc
    42721 aatggcataa gctcttctgt ttcatctact gcaaatccaa acatgagccc ttggtcacct
    42781 gctccaatca agtccagtgg atcttgatca gcatttccgc gaacctccaa ggcttcgtta
    42841 acaccttgag cgatgtcagg agattgttcc accaaagatg ggtgtactcc caccgtctca
    42901 gcagaaaatc catattctgt attggtataa ccaatctctg caatggtatc acgaaccaca
    42961 cggttaatat ccacataggc atttgtagaa atttcaccaa aaacgtggac agaaccagta
    43021 tatacagctg tttcagcagc aacgtgcgcc tctggatcct ttgctaaaat agcatccaaa
    43081 atcgcatctg aaatttggtc tgcaatctta tccggatgcc cctcagatac agattcagac
    43141 gtgaataatt tacgttctga cataaaaatg tcccccctta aaaaatattg ttatagagtt
    43201 acaaagtact gataggaaaa ctcttcgtaa taaataccta ccatcttatc gggactatat
    43261 aactttatca ttataccatt tttaatcgca atttgcagtc tttaatgtag tgtttcggtt
    43321 tagatagttc gttctcataa agcaaaaagt tggagctcag tactccaaca atttctatta
    43381 aaatgtttgg cgtttagctt tacgctctgc acgaccgcga gcgcgatttt cagcacgctt
    43441 agttttacgg cgcttttcat caaccgccca ttgaattttc ttcttataac ccggtttgac
    43501 ttttttcttt ttctttttaa ccaaaccaat catttcgata tcaagtttat cttgtttttt
    43561 ctcacggttg gcacgacgat cacggtcata ggtatcttga aattccccat ctttgaccat
    43621 cttaggacta aacttgattc ccaatttctc caactcacgg atatccgagt catcacttgg
    43681 ctgataaagg gtaatagctg tacctggtag gccatttcgt ccagtacgac caacacggtg
    43741 aacaaaaaaa gataagtctt gcggaatggc atcattgatg acatggctga caccttcaat
    43801 gtcaatccca cgcgctgcta aatctgttgc gacaatatac tcaaaatcca gattttgcac
    43861 ctgattcatg attcgcttgc gttcacgagg ggcaatatcg ccatggattt ttgcaacctt
    43921 caagccttga gcagtcagat atgaatgcaa ttcatcagca cgcgttttag tgttaacaaa
    43981 aatcattgcc aaatacggct gcatcaactg agtcaactgg taaatttgag cattcttatc
    44041 atgtcccttg gtcgaaatca accaattatc aatggtgtca gaaataaccg ttttagtctt
    44101 aattttctcc ataacaggat ttgataagta ttttttcaag aatggttgca gtttttgtgg
    44161 gatagtcgct gagaagacca tgaattgcaa gtcttttgga agactgccag caatcttatc
    44221 aacagtttcc aagaatccca tatccaaggt catatctgct tcatcaacaa caaatgtctt
    44281 ggctttatga atagctaaat caccagattt aaccaagtcg tagatacggc ctggtgttcc
    44341 aataacaata tgaggctgat tgctttccaa tttctcaatc tggcgagcct tatccgtacc
    44401 acccacataa ttaaccacac gaacttcgac atctgagtga gctgaaatct gacgcgctac
    44461 ttggtaaatt tgagtagcca actcacgact cggtgcagta atcactgctt gtacactatc
    44521 gctagcttca tctaattgct ggaaaatcgg taacaagaaa gtgtgagtct tacctgaacc
    44581 tgtttttgat tctcctacta ggtcacgacc tgccaaaaca ataggaatca acttgtcttg
    44641 cacctctgtt ggagttgtaa attttaactc cttcaaggct tctctaatat agtttttaaa
    44701 ttgaaatttc gtaaatgaca taacatcctc gattctatct atcttatcaa ttataccata
    44761 ttttattcca ttacagtagt ctcacttatt taggctattt ccagtagctt ctctagtaag
    44821 aaaaggctgg aattttatag ttccaacctc ttttcagtta ttatttccag tttaacatag
    44881 cattcaagcc atagtgatca ctcacttgtg gactcttgtt accatcaaat acgacatgta
    44941 aattttccac cgctaactct ttggtagtaa agacataatc gattcgaagg ggttcagtgt
    45001 tccctttcca gccatcaatt tcaggcggaa cagtatagct accacttttc tcttgagcaa
    45061 cttcaaatgc gtcttgtaag cctaatggac tagctaaaat agcttggtaa ccttcctgac
    45121 ctgctgggtt gttaaaatct ccagctagta aaagtggctt gttcaatttt ttcaagacaa
    45181 cctcaaatcg tgcccattct tcttgaaaac ctttatccca ccaagagaga tgaacactgg
    45241 caactgctag ctccttgcca tcgactacag tttcagctag ggcaacacgg cgagtatgat
    45301 agtctgttgg atcatccaca tctgaaacca aaatttctct ggcttcaata ggtgttttag
    45361 acaagatagc cacaccttcg tggtagcggt catagccgat atggttatag gcccaggtcc
    45421 agtagtaatt tttcccttgc tcagataact tttcaaccaa gagtctaaca tagtgatctt
    45481 ggtgaatagg ctcagctgct ggcaaagctt gataaaggtc attaacctcc acctctgacg
    45541 aggtcatctc ctgattaatt tcttggaaac aaatcaaatc atagtccttt tcaagaatat
    45601 cttcaagcaa aatctggaat ttttcctctg cttctttctc catccaacta tgagtattga
    45661 gtgttaaaaa tttcattctt ttctcccaaa ataagaggtt ggaatacagc ttccaacctc
    45721 aagtatatta caattctact ttagcaactg ctgttttagc tgcaagagaa cctgtttttt
    45781 ctaatttaac tgatttaatt gcatcaccat ttgtgaagac aactactgtt gaagtttcac
    45841 gtcctgctgc acggatagca ttcaagtcag ctgtgacaag gagatctcct gctgcaactt
    45901 tttgtccttc agcaacatga actgtaaatg gtttaccttc aagacttact gtgtccaaac
    45961 caatgtgaac caatacttca agacctgctt ccgtcacaat accaaaagca tgttttgttg
    46021 ggaagatgct tgatacagta cctgaaactg gagatacaat gtttccattt gcaggttcta
    46081 ctgcaaatcc atcacccatc attttttgag caaatactgg atcctttact tgttccaaag
    46141 caacaacttg accgtctgct actgagtaaa cttcctcagt aagatctttg aagtgaacag
    46201 tgttttgttg tacttcagtc atttggcttg gaagagtttc aggaatgatt tcacctgaat
    46261 caaggatatc ttggatatca gatttcaaaa tgtcagcttt tggaccgtag atagcttgaa
    46321 ccccttgtcc tttcatcaca agacccatag ctccttctgc tttccattgc tctgcatttc
    46381 ctactttatc tgcatcttta acagttacac gaagacgagt catacatgca tcaacatcaa
    46441 cgatgtttac acgtccacca agaaggttga taatgtttac agcttgagag cctgctgcaa
    46501 ctttcacttc gctgctggtt tcttctgaac cttcagcagt ttcgtagttt ccgttgcgcc
    46561 ctggagttgc gtagttgaat ttttgaatca tgaagtttgc gataaagtac atgattacag
    46621 caaagagaac agttacccaa acgaagttaa cgatatccat accaatacca gcactgattg
    46681 caataggtgt acgagtcaag aactcgattg aaccgaatga gtgcatacgt aggtttacga
    46741 cgtcagccat agcgaaggca gcaccttgaa caagtgagta aacaagatac ataggtgttg
    46801 cgatgaacat gaacatgtat tcgattggtt cagtaacccc tgtcaagaat gttgcaagag
    46861 ctgttgcaat catcatacct ttgtatttat gtttcttgtc agcatcaaca ttacggtaga
    46921 tagcaacaat cacacccatc aagataccga atgaaccgat catttgtcca actttgaaac
    46981 gagctggatg tactgtatct aacaagtgtt ggtattgact agcatcagta cctttaaggt
    47041 ttacaaggtc tgttacccat gcaagccata gtgggtcttg accgaatact tgagtacctt
    47101 tagctgcacc agttaaaatg tcataagtac caccaagagc tgtgtagttc attgggatag
    47161 tcaacatgtg gtgaagacca aatggcaaga gcaaacgttc caaagtacca tacaagaatg
    47221 gtgcaagaat tggagcagtt tcttgtgagt tggcaatcca gataccgaaa ttattgatac
    47281 ctgtttgaac tactggccag aaagcagcaa gtagaattgc agcgattgct gaacgaagaa
    47341 taactacaaa tggtacgaaa cgtttcccgt tgaagaatga aagtgcatca ggaagtttac
    47401 ggaagttgta gtatttgttg taagcagttg cccctacaaa acctgagata atccctacga
    47461 atacccccat gttcaaggct ggagcttcaa gaacactgat aaagtaatca gcaactttga
    47521 ttgaaccacc aaagaaagta gttaccatag catctggatt tttcaacata tcgcctgata
    47581 caccaaagat tgtaccagtg atacggttaa tcaagatgaa ggcaagacca gcggcgaaag
    47641 caccaccagc acgttcttta gcccagcttc ctccaatggc tagggcaaac aaaatgtgaa
    47701 ggttaccgat aaccccccaa ccgatttgct caagaattcc acctgtgatg acaagtggtg
    47761 caaaggttgg gttaatcatc acgatagact taccgattga aatcatcaaa ccagcagccg
    47821 gcataactgc gataactacc atcaaagcct taccgaattt ttgccaaaat tcgaaagaca
    47881 agacattttt gaatgtatct ttcatcattc aaatctcctt tatttttatt taggcaaacg
    47941 ttttacgaaa acgtttgcac
//
LOCUS       DAVLVZ010000009        45576 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000009 DAVLVZ010000000
VERSION     DAVLVZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.009"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            573..1025
                     /locus_tag="VYN77_000475"
     CDS             573..1025
                     /locus_tag="VYN77_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1557982.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFIYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1044..2381
                     /locus_tag="VYN77_000476"
     CDS             1044..2381
                     /locus_tag="VYN77_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1557983.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2460..3344
                     /locus_tag="VYN77_000477"
     CDS             2460..3344
                     /locus_tag="VYN77_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1557984.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3344..4177
                     /locus_tag="VYN77_000478"
     CDS             3344..4177
                     /locus_tag="VYN77_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1557985.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4195..6288
                     /gene="nanB"
                     /locus_tag="VYN77_000479"
     CDS             4195..6288
                     /gene="nanB"
                     /locus_tag="VYN77_000479"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1557986.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     NETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6300..7403
                     /locus_tag="VYN77_000480"
     CDS             6300..7403
                     /locus_tag="VYN77_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1557987.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7577..8275
                     /locus_tag="VYN77_000481"
     CDS             7577..8275
                     /locus_tag="VYN77_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1557988.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8294..9811
                     /locus_tag="VYN77_000482"
     CDS             8294..9811
                     /locus_tag="VYN77_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1557989.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALAVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9936..11264
                     /locus_tag="VYN77_000483"
     CDS             9936..11264
                     /locus_tag="VYN77_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1557990.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11517..12383
                     /locus_tag="VYN77_000484"
     CDS             11517..12383
                     /locus_tag="VYN77_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1557991.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12399..13238
                     /locus_tag="VYN77_000485"
     CDS             12399..13238
                     /locus_tag="VYN77_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1557992.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13511..13963
                     /locus_tag="VYN77_000486"
     CDS             13511..13963
                     /locus_tag="VYN77_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1557993.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14105..14413
                     /locus_tag="VYN77_000487"
     CDS             14105..14413
                     /locus_tag="VYN77_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1557994.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LNRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDAHDKIPNNGRINF
                     "
     gene            14697..15341
                     /locus_tag="VYN77_000488"
     CDS             14697..15341
                     /locus_tag="VYN77_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1557995.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTAPPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15510..16427
                     /locus_tag="VYN77_000489"
     CDS             15510..16427
                     /locus_tag="VYN77_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1557996.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLVGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16445..17329
                     /locus_tag="VYN77_000490"
     CDS             16445..17329
                     /locus_tag="VYN77_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1557997.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17345..18196)
                     /locus_tag="VYN77_000491"
     CDS             complement(17345..18196)
                     /locus_tag="VYN77_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1557998.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18396..20453
                     /gene="pbp2b"
                     /locus_tag="VYN77_000492"
     CDS             18396..20453
                     /gene="pbp2b"
                     /locus_tag="VYN77_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1557999.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELGNGGAKYSEGVYAVALNPKTGAVLSMSGLKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQPIVFQGSAPIYSWYKLAYGSFPITAVEALEYSSN
                     AYMVQTALGIMGQTYQPNMFVGTSNLETAMGKLRATFGEYGLGAATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVAGGQEANNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYNQHH
                     PMN"
     gene            20464..21060
                     /gene="recR"
                     /locus_tag="VYN77_000493"
     CDS             20464..21060
                     /gene="recR"
                     /locus_tag="VYN77_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1558000.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSDDDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21236..22279
                     /locus_tag="VYN77_000494"
     CDS             21236..22279
                     /locus_tag="VYN77_000494"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1558001.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSQTPGQEDRLMTNATIDWDKQVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLTYPVFTKPSNMGSSVGISKSENQEELRQALKLAFQYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRRNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPDLIEHLVDL
                     AKESFDKREAHLL"
     gene            22364..23737
                     /locus_tag="VYN77_000495"
     CDS             22364..23737
                     /locus_tag="VYN77_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1558002.1"
                     /translation="MKLTIHEVAQAVGAKNNVSIFEDIQLEKVEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTTYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELAHPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMAPGSLLLAP
                     ADSIVEAYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRQALQNLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTDDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLEHEDK"
     gene            23724..24335
                     /locus_tag="VYN77_000496"
     CDS             23724..24335
                     /locus_tag="VYN77_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1558003.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRGLLSEML
                     NQASDLDAEEVAEVLNPTSAYATPLMDVRAWIVEEEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKFLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIKILWQVYQGQREQYLD"
     gene            24426..25130
                     /locus_tag="VYN77_000497"
     CDS             24426..25130
                     /locus_tag="VYN77_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1558004.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPCPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25349..26722
                     /gene="ftsA"
                     /locus_tag="VYN77_000498"
     CDS             25349..26722
                     /gene="ftsA"
                     /locus_tag="VYN77_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1558005.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26739..27998
                     /gene="ftsZ"
                     /locus_tag="VYN77_000499"
     CDS             26739..27998
                     /gene="ftsZ"
                     /locus_tag="VYN77_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1558006.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVSPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28003..28674
                     /locus_tag="VYN77_000500"
     CDS             28003..28674
                     /locus_tag="VYN77_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1558007.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28684..29223
                     /locus_tag="VYN77_000501"
     CDS             28684..29223
                     /locus_tag="VYN77_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1558008.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29223..29486
                     /locus_tag="VYN77_000502"
     CDS             29223..29486
                     /locus_tag="VYN77_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1558009.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29483..30268
                     /locus_tag="VYN77_000503"
                     /pseudo
     CDS             29483..30268
                     /locus_tag="VYN77_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            30277..31065
                     /gene="divIVA"
                     /locus_tag="VYN77_000504"
     CDS             30277..31065
                     /gene="divIVA"
                     /locus_tag="VYN77_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1558010.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPVPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVEAPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31413..34205
                     /locus_tag="VYN77_000505"
     CDS             31413..34205
                     /locus_tag="VYN77_000505"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1558011.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFADAVAEGFEEK"
     gene            34614..34976
                     /locus_tag="VYN77_000506"
     CDS             34614..34976
                     /locus_tag="VYN77_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558012.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            35511..36203
                     /locus_tag="VYN77_000507"
     CDS             35511..36203
                     /locus_tag="VYN77_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1558013.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            36256..38667
                     /locus_tag="VYN77_000508"
     CDS             36256..38667
                     /locus_tag="VYN77_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1558014.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASNGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     RGQEYIEAARASLNDREDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSAMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            38917..39618
                     /locus_tag="VYN77_000509"
     CDS             38917..39618
                     /locus_tag="VYN77_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558015.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNSDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            39620..42328
                     /locus_tag="VYN77_000510"
     CDS             39620..42328
                     /locus_tag="VYN77_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1558016.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQAEQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSVASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(42424..42915)
                     /gene="tpx"
                     /locus_tag="VYN77_000511"
     CDS             complement(42424..42915)
                     /gene="tpx"
                     /locus_tag="VYN77_000511"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1558017.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(43038..43967)
                     /gene="psaA"
                     /locus_tag="VYN77_000512"
     CDS             complement(43038..43967)
                     /gene="psaA"
                     /locus_tag="VYN77_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1558018.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(43999..44847)
                     /locus_tag="VYN77_000513"
     CDS             complement(43999..44847)
                     /locus_tag="VYN77_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1558019.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(44844..45566)
                     /locus_tag="VYN77_000514"
     CDS             complement(44844..45566)
                     /locus_tag="VYN77_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1558020.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT        13840 a         8164 c        10036 g        13536 t
ORIGIN      
        1 aattgaaaac ggtaacaaaa ctaatttaga gaatgaaata tagagtattt ctctcttaaa
       61 agtttttggt gaaacgagat gtagaaagga gatttagcca aagagtctat tagtgctaga
      121 ataatagatt agaattattt tagaaaaacg aagtgagcag cttataaatt caagtcccca
      181 aatagattca tactagtatc ttttgcaaaa aataaagggc gacttccttc atgaatatca
      241 atttcatcta taaggaaggt agctaattga actaacttat ttattctgtt tgtcgctaga
      301 aaaatcagac ctccttgtga agattgagga gatactgaat gaaaatcaaa gaagaaacta
      361 gcaagctagt agcagattgc ccaaaacacc gctttgaggt tgtagataag actgacgtat
      421 ataatccaag gtgaagcgac tgtggtttga agagattttc aaagagtata ggctagagag
      481 tagtgttttt atgtccttct agtagaaaat gctagacaga agaatgggga acttggatag
      541 gaaaaataga ttgagaaagg aggttagaag agatgattat tacaaaaatt agccgtttag
      601 gaacttatgt gggagtcaat ccacattttg caacattaat agattttcta gaaaaaacag
      661 gactagaaaa tttaacagaa ggttcgattg ctatcgatgg taatcgattg tttgggaatt
      721 gctttattta tctagcagat ggtcaagcag gggctttctt tgaaacacac caaaaatatt
      781 tggatattca tttagttttg gaaaacgaag aagccatggc tgttacatcg ccggaaaatg
      841 taagcgttac ccaagaatat gatgaagaga aagatattga attatacaca gggaaagtgg
      901 aacagttggt tcatttgaga gctggtgaat gcctcatcac ttttccagaa gatttacatc
      961 aacccaaggt tcgtataaat gatgaacctg tgaaaaaagt tgtctttaaa gttgcgattt
     1021 cttaatgtag aaagagaaga acgatgaaaa aaatgagaaa gtttttatgt ctagctggaa
     1081 ttgcgctagc ggctgttgcc ttggtagctt gttcaggaaa aaaagaagct acaactagta
     1141 ctgaaccacc aacagaatta tctggtgaaa ttacaatgtg gcactccttt actcaaggac
     1201 cccgtttaga aagtattcaa aaatcagcag atgctttcat gcaaaagcat ccaaaaacga
     1261 aaatcaagat tgaaacattt tcttggaatg acttctatac taaatggact acaggtttag
     1321 caaatggaaa tgtgccagat atcagtacag ctcttcctaa ccaagtaatg gaaatggtca
     1381 actcagatgc tttggttccg ctaaatgatt ctatcaagcg tattggacaa gataaattta
     1441 acgaaactgc cttaaatgaa gcaaaaatcg gagatgatta ctactctgtt cctctttatt
     1501 cacatgcaca agtcatgtgg gttagaacag atttgttaaa agaacataat attgaggttc
     1561 ctaaaacttg ggatcaactc tatgaagctt ctaaaaaatt gaaagaagct ggagtttatg
     1621 gcttgtctgt tccgtttgga acaaatgact taatggcaac acgtttcttg aacttctacg
     1681 tacgtagtgg tggaggaagc ctcttaacaa aagatcttaa agcagacttg acaagccaac
     1741 ttgctcaaga tggtattaaa tactgggtta aattgtataa agaaatctca cctcaagatt
     1801 ctttgaattt taatgtcctt caacaagcta ccttgttcta tcaaggaaaa acagcatttg
     1861 actttaactc tggcttccat atcggaggaa ttaatgccaa cagtcctcaa ttgattgatt
     1921 cgattgatgc ttatcctatt ccaaaaatca aagagtctga taaagaccaa ggaattgaaa
     1981 cctcaaacat tccaatggtt gtttggaaaa attcaaaaca tccagaagtt gctaaagcat
     2041 tcttagaagc actttataat gaagaagact acgttaaatt ccttgattca actccagtag
     2101 gtatgttgcc aactattaag gggattagcg attctgcagc ctataaagaa aatgaaactc
     2161 gtaagaagtt taaacatgct gaagaagtaa ttactgaagc tgttaaaaaa ggtactgcta
     2221 ttggttatga aaatggacca agtgtacaag ctggtatgtt gactaaccaa cacattattg
     2281 aacaaatgtt ccaagatatc attacaaatg gaacagatcc tatgaaagca gcaaaagaag
     2341 cagaaaaaca attaaacgat ttatttgagg ctgttcagta gatgtaaaag actagaaaat
     2401 aggtgggcta gtgatctgaa aagctctagc ccaattttgt aaaagaaggg agaaggagaa
     2461 tggttaaaga acgtaattta actcgctgga tatttgtttt gccagctatg attatcgtag
     2521 gattactctt tgtttatccg tttttctcga gtatttttta tagctttacc aataagcatt
     2581 tgattatgcc taattataaa tttgttggtt tggctaacta taaagctgtg ctatcagatc
     2641 ccaacttctt taatgcgttc tttaattcaa ttaagtggac cgttttctca ttagttggtc
     2701 aagttttagt agggtttgta ttggctttag ctcttcacag agtacgccac ttcaagaaat
     2761 tatataggac cttattgatt gttccttggg catttcctac catcgttatt gccttctctt
     2821 ggcagtggat tctaaacggg gtttatggct acttacctaa tctaatcgta aaattaggtt
     2881 taatggaaca tacacctgca tttttgacag atagtacatg ggcattccta tgtttggtgt
     2941 ttatcaacat ttggtttgga gcaccaatga ttatggttaa tgtgctttca gctttgcaaa
     3001 cagtaccaga agaacaattt gaggctgcta agatagatgg tgcttcaagt tggcaggtgt
     3061 tcaagtttat cgtctttcca catattaaag tggttgtagg acttctagtt gttttgagaa
     3121 ctgtatggat ctttaataac tttgacatta tctacctcat tactggtggt ggaccagcca
     3181 atgctacaac gacgcttcca atttttgctt acaacctggg ctggggaact aaattgttgg
     3241 gtcgtgcttc agcagttaca gtactgctct ttatcttctt ggtggcgatt tgctttatct
     3301 actttgctat catcagtaag tgggaaaagg agggtagaaa ataatgaaga agaaatccag
     3361 tatttattta gatattctct cacatgtact tttagttggt gcgaccatcg ttgcagtttt
     3421 cccattggta tggattatca tatcttctgt caaagggaaa ggggaattaa ctcagtatcc
     3481 aacacgattt tggcctgaac agtttacatt agattatttc actcatgtta tcaacgattt
     3541 gcacttcatt gataacattc gaaacagttt aatcattgcc ttggctacaa cccttattgc
     3601 gattattatt tctgctatgg cagcctatgg tattgttcga ttctttccta aattgggagc
     3661 aatcatgtcg agactactcg tcattaccta cattttccca ccaattttgt tagcaattcc
     3721 ctattcaatt gccattgcta aagttgggtt aacaaatagt ttatttggct tgatgatggt
     3781 ttatctatct tttagtgttc catatgcagt ttggctctta gttggatttt tccaaacagt
     3841 tccaattgga attgaagaag cggctagaat tgatggtgca aataaatttg ttacgtttta
     3901 taaagttgtg ctaccgattg tagcaccagg tattgtagca acagctattt atacatttat
     3961 caatgcttgg aatgaattcc tgtatgcctt gattttgatt aacaatacag gaaagatgac
     4021 agtagcagta gcccttcgtt cacttaatgg ttcagaaata ctagactggg gagatatgat
     4081 ggcagcgtct gttattgtag ttcttccatc aattattttc ttctctatca tccaaaataa
     4141 gattgcaagt ggattatcag aaggatctgt gaagtagacg aaagaaggaa aaaaatgaat
     4201 aaaagaggtc tttattcaaa actaggaatt tccgttgtag gcattagtct tttaatggga
     4261 gtccccactt tgattcatgc gaatgaatta aactatggtc aactgtccat atctcctatt
     4321 tttcaaggag gttcatatca actgaacaat aagagtatag atatcagctc tttgttatta
     4381 gataaattgt ctggagagag tcagacagta gtaatgaaat ttaaagcaga taaaccaaac
     4441 tctcttcaag ctttgtttgg cctatctaat agtaaagcag gctttaaaaa taattacttt
     4501 tcaattttca tgagagattc tggtgagata ggtgtagaaa taagagacgc ccaaaaggga
     4561 ataaattatt tattttccag accagcttca ttatggggaa aacataaagg acaggcagtt
     4621 gaaaatacac tagtatttgt atctgattct aaagataaaa catacacaat gtatgttaat
     4681 ggaatagaag tgttctctga aacagttgat acatttttgc caatttcaaa tataaatggt
     4741 atagataagg caacactagg agctgttaat cgtgaaggta aggaacatta cctcgcaaaa
     4801 ggaagtattg atgaaatcag tctatttaac aaagcaatta gtgatcagga agtttcaact
     4861 attcccttgt caaatccatt tcagttaatt ttccaatcag gagattctac tcaagctaac
     4921 tattttagaa taccgacact atatacatta agtagtggaa gagttctatc aagtattgat
     4981 gcacgttatg gtgggactca tgattctaaa agtaagatta atattgccac ttcttatagt
     5041 gatgataatg ggaaaacgtg gagtgagcca atttttgcta tgaagtttaa tgactatgag
     5101 gagcagttag tttactggcc acgagataat aaattaaaga atagtcaaat tagtggaagt
     5161 gcttcattca tagattcatc cattgttgaa gataaaaaat ctgggaaaac gatattacta
     5221 gctgatgtta tgcctgcggg tattggaaat aataatgcaa ataaagccga ctcaggtttt
     5281 aaagaaataa atggtcatta ttatttaaaa ctaaagaaga atggagataa cgatttccgt
     5341 tatacagtta gagaaaatgg tgtcgtttat aatgaaacaa ctaataaacc tacaaattat
     5401 actataaatg ataagtatga agttttggag ggaggaaagt ctttaacagt cgaacaatat
     5461 tcggttgatt ttgatagtgg ctctttaaga gaaaggcata atggaaaaca ggttcctatg
     5521 aatgttttct acaaagattc gttatttaaa gtgactccta ctaattatat agcaatgaca
     5581 actagtcaga atagaggaga gagttgggaa caatttaagt tgttgcctcc gttcttagga
     5641 gaaaaacata atggaactta cttatgtccc ggacaaggtt tagcattaaa atcaagtaac
     5701 agattgattt ttgcaacata tactagtgga gaactaacct atctcatttc tgatgatagt
     5761 ggtcaaacat ggaagaaatc ctcagcttca attccgttta aaaatgcaac agcagaagca
     5821 caaatggttg aactgagaga tggtgtgatt agaacattct ttagaaccac tacaggtaag
     5881 atagcttata tgactagtag agattctgga gaaacatggt cgaaagtttc gtatattgat
     5941 ggaatccaac aaacttcata tggcacacaa gtatctgcaa ttaaatactc tcaattaatt
     6001 gatggaaaag aagcagtcat tttgagtaca ccaaattcta gaagtggccg taagggaggt
     6061 caattagttg tcggtttagt caataaagaa gatgatagta ttgattggaa ataccactat
     6121 gatattgatt tgccttcgta tggttatgcc tattctgcga ttacagaatt gccaaatcat
     6181 cacataggtg tactgtttga aaaatatgat tcgtggtcga gaaatgaatt gcatttaagc
     6241 aatgtagttc agtatataga tttggaaatt aatgatttaa caaaataaag gagaaaaaca
     6301 tggttaaata cggtgttgtt ggagcagggt attttggagc tgaattggct cgctacatgc
     6361 aaaagaatga tggagcagag attactcttc tctatgatcc agataatgca gaggcgattg
     6421 cagaagaatt gggggcaaaa gtagcaagtt ccttagatga gttggtttct agcgatgaag
     6481 tagattgtgt tatcgtcgca actccaaata atcttcataa ggaaccggtt attaaggctg
     6541 cacagcatgg taaaaatgtt ttctgtgaaa aaccaattgc gctttcttat caagattgtc
     6601 gcgagatggt agatgcgtgt aaagaaaaca atgtaacctt tatggcagga catattatga
     6661 atttctttaa tggtgttcat catgcaaaag aactcattaa tcaaggagtt atcggagacg
     6721 ttctatattg tcatacagct cgtaatggtt gggaagaaca acaaccgtca gtatcatgga
     6781 aaaaaattcg tgaaaaatca ggtggtcact tgtatcacca catccatgaa ttggattgcg
     6841 ttcaattcct tatggggggc atgcctgaaa ctgtaaccat gacaggtgga aatgtggccc
     6901 atgaaggtga acatttcggt gatgaagatg atatgatttt tgtcaatatg gaattttcta
     6961 ataagcgttt tgctttgtta gaatggggtt cagcttatcg ttggggtgaa cattatgtct
     7021 taatccaagg aagcaaaggt gccatccgct tagacttatt caactgtaaa ggaactctta
     7081 agctagatgg gcaagaaagc tatttcttga ttcacgaatc gcaagaagaa gatgatgatc
     7141 ggactcgtat ctatcatagt acagagatgg atggagcaat tgcttatggt aaaccaggta
     7201 aacgtactcc attatggcta tcatctgtca ttgataaaga aatgcgctat ctgcatgaga
     7261 ttatggaagg agctccagta tcagaagaat ttgcaaaact tttgacaggt gaagctgccc
     7321 tagaagcaat tgctactgca gatgcttgta cccagtctat gtttgaagat cgcaaagtaa
     7381 aattgtcaga aattgtaaaa taaattttgg tattctccta tttataggtc gacttgctcc
     7441 tctgaaagta cttttagagg agctgtttga ctttgctagt ttttgaaact gaaatctatt
     7501 atactacaaa ctattgaaag cgttttaatt ttaaggtata ataatctcat agaaataaag
     7561 aaaaggagga aagaggatgc cacagattag caaagaagcc ttgattgagc aaatcaaaga
     7621 tggaatcatc gtttcttgtc aggctcttcc tcatgaaccg ctttatacag aagcgggagg
     7681 ggtgattccc ttgctggtca aagcggctga gcaaggtgga gcagtcggta tccgagcaaa
     7741 cagtgttcgc gatatcaagg aaattaagga agtcactaaa cttccaatca ttgggattat
     7801 caaacgtgat tatccacctc aggaaccctt catcacggct actatgaaag aagttgatga
     7861 attggcagaa ctggacatcg aggtgattgc tctggattgt accaagcgtg aacgctacga
     7921 tggtttggaa attcaagagt tcattcgtca ggttaaggag aaatatccta atcagctttt
     7981 gatggctgat actagtatct tcgaagaagg gctagcagct gtagaagcag gaattgactt
     8041 tgtcggaaca accttatcag gctacacatc ctacagtcca aaagtagacg gtccagattt
     8101 tgaattgatt aagaaactct gtgatgctgg tgtagatgtc attgcagaag gaaaaattca
     8161 tacaccagaa caagccaaac aaatccttga atatggagtg cgaggcatcg ttgttggtgg
     8221 cgccattact agaccaaaag agattacaga acgcttcgtt gctagtctta aataagatgt
     8281 gagggggagt tttatgttta aagttttaca aaaagttgga aaagctttta tgttacctat
     8341 agctatactt cctgcagcag gtctactttt ggggattggt ggtgcacttt caaacccaac
     8401 cacgatagca acttatccaa tactagacaa tagtattttt caatcgatat tccaagtaat
     8461 gagctctgca ggagaggttg tattcagtaa tttgtcacta cttctctgtg tgggattatg
     8521 tattggctta gcgaaacgag ataaaggaac cgctgcgtta gcaggagtaa ctggttactt
     8581 agttatgact gcaacgatca aagctttggt aaaacttttt atggcagaag gatctgcaat
     8641 tgatactgga gttattggag cattagctgt cggaatagtt gccgtatatt tgcacaaccg
     8701 atataacaat attcaattac cttccgcttt aggattcttt ggaggttcac gcttcgttcc
     8761 tattgttaca tcgttctctt ctatcttgat tggctttgtc ttctttgtta tttggccacc
     8821 tttccaacaa cttcttgttt ctacaggtgg atatatttct caggcgggtc caattggaac
     8881 ttttctatat ggatttttaa tgagactttc tggagcagta ggcttacatc atataattta
     8941 ccctatgttt tggtatactg aacttggtgg tgttgaaact attgcaggac aaacagtggt
     9001 tggagctcaa aaaatatttt ttgctcaatt agccgatccg gcccattctg gattatttac
     9061 agaaggaaca aggttttttg caggtcgttt ctcaacaatg atgttcggtt taccggctgc
     9121 ctgtttagcg atgtaccata gtgttcctaa aaatcgtcgt aaaaaatacg cgggtttgtt
     9181 ttttggagtt gctttaacat cttttattac cggtattaca gaaccaattg aatttatgtt
     9241 tctattcgtc agtccggttc tatatgttgt tcacgcattc cttgatggtg ttagcttctt
     9301 tattgcagac gtcttaaata tttcaatagg aaacacattt tcaggaggtg taatcgattt
     9361 cactttattt ggaattttgc aggggaacgc taagacgaat tgggttcttc agattccaat
     9421 tggacttatt tggagtgttt tgtattatat tatttttaga tggttcatta ctcaattcaa
     9481 cgttctaacg ccaggacgag gagaagaagt agattctaaa gaaatttctg aatccgcaga
     9541 ttcaacttca aatactgcag attatttaaa acaggatagc ctacaaatta tcagagcgtt
     9601 gggtggatca aataatatag aagatgtaga tgcttgtgtg acacgtttac gtgtagctgt
     9661 aaaagaagtt aatcaagttg ataaagcact tttaaaacaa attggtgcag ttgatgtctt
     9721 agaagtgaag ggtggcattc aagcaatcta tggagcaaaa gcaatcttat ataaaaatag
     9781 tattaatgaa attttaggtg tagatgatta agtacttact gacttaataa aaaacagagg
     9841 agagtgatgg atgagtagga tgaaatgaaa tcgcatacaa gaaataaaga actcattatc
     9901 caagttggat acgcttatta cataggagaa tacaaatgaa atttagaaaa ttagcttgta
     9961 cagtacttgc gggtgctgcg gttcttggtc ttgctgcttg tggcaattct ggcggaagta
    10021 aagatgctgc caaatcaggt ggtgacggtg ccaaaacaga aatcacttgg tgggcattcc
    10081 cagtatttac ccaagaaaaa actggtgacg gtgttggaac ttatgaaaaa tcaatcatcg
    10141 aagcgtttga aaaagcaaac ccagatataa aagtgaaatt ggaaaccatc gacttcaagt
    10201 caggtcctga aaaaatcaca acagccatcg aagcaggaac agctccagac gtactctttg
    10261 atgcaccagg acgtatcatc caatacggta aaaacggtaa attggctgag ttgaacgacc
    10321 tcttcacaga tgaatttgtt aaagatgtca acaatgaaaa catcgtacaa gcaagtaaag
    10381 ctggagacaa ggcttatatg tatccgatta gttctgcccc attctacatg gcaatgaaca
    10441 agaaaatgtt agaagatgct ggagtagcaa accttgtaaa agaaggttgg acaactgatg
    10501 attttgaaaa agtattgaaa gcacttaaag acaagggtta cacaccaggt tcattgttca
    10561 gttctggtca agggggagac caaggaacac gtgcctttat ctctaacctt tatagcggtt
    10621 ctgtaacaga tgaaaaagtt agcaaatata caactgatga tcctaaattc gtcaaaggtc
    10681 ttgaaaaagc aactagctgg attaaagaca atttgatcaa taatggttca caatttgacg
    10741 gtggggcaga tatccaaaac tttgccaacg gtcaaacatc ttacacaatc ctttgggcac
    10801 cagctcaaaa tggtatccaa gctaaacttt tagaagcaag taaggtagaa gtggtagaag
    10861 taccattccc atcagacgaa ggtaagccag ctcttgaata tcttgtaaac gggtttgcag
    10921 tattcaacaa taaagacgac aagaaagtcg ctgcatctaa gaaattcatc cagtttatcg
    10981 cagatgacaa ggagtgggga cctaaagacg tagttcgtac aggtgctttc ccagtccgta
    11041 cttcatttgg aaaactttat gaagacaaac gcatggaaac aatcagtggc tggactcaat
    11101 actactcacc atactacaac actattgatg gatttgctga aatgagaaca ctttggttcc
    11161 caatgttgca atctgtatca aatggtgacg aaaaaccagc ggatgctttg aaagccttca
    11221 ctgaaaaagc aaacgaaaca atcaaaaaag ctatgaaaca atagtcctta gttattctat
    11281 gaaaaagtaa ttttttaaag aacctaagag tgtatacccc cttttccctc tacacagata
    11341 gtgtaagaaa agggggcttt tgtttaaaat gtaagaaact gtcacgaaat taaaatgaag
    11401 ttcttacata agcgaaccat aaaaaatttc attttgattt taaaacagtt caagaaagtc
    11461 aaaaaattat tctatttgaa agagaggtgc cgactgtgaa agtcaataaa atccgtatgc
    11521 gggaaacagt gatttcctac gctttcctag caccagtatt attcttcttt gtcatatttg
    11581 tgttggctcc gatggtgatg ggcttcatta caagtttctt taactactca atgactaaat
    11641 ttgagtttgt aggcttggat aactatatcc gtatgtttaa agatcctgtc tttacaaaat
    11701 ctctgattaa cacagttatt ttggttattg gatctgtacc agttgttgtt ctattctcac
    11761 tctttgtagc atctcagacc tatcatcaaa atgtcattgc cagatccttc taccgtttcg
    11821 tcttcttcct tcctgttgta acgggtagtg ttgccgtgac agttgtttgg aaatggattt
    11881 atgacccact atcagggatt ctaaactttg tccttaagtc cagccacatc atcagccaaa
    11941 acatttcttg gttgggagat aaaaactggg cattgatggc gattatgatt attctcttga
    12001 ccacttcagt tggtcagccc atcatccttt atatcgctgc catggggaat attgacaatt
    12061 cactggttga agcggcgcgt gttgatggtg caactgagtt tcaagttttt tggaagatta
    12121 aatggccaag ccttcttcca acaactcttt atattgcaat catcacaaca attaactcat
    12181 tccagtgttt cgccttgatt cagcttttga catctggtgg tccaaactac tcaacaagta
    12241 ccttgatgta ctacctttac gaaaaagcct tccaattgac agaatacggc tatgccaaca
    12301 caattggtgt cttcttggca gtcatgattg ctatcgtaag ctttgttcaa tttaaagtac
    12361 ttggaaacga cgtagaatac taaagaaagg agacagctat gcaatctaca gaaaaaaaac
    12421 cattaacagc ctttactgtt atttcaacaa tcattttgct cttgttgact gtgctgttca
    12481 tctttccatt ctactggatt ttgacagggg cattcaaatc acaacctgat acaattgtta
    12541 ttcctcctca gtggttccct aaaatgccaa ccatggaaaa cttccaacaa ctcatggtgc
    12601 agaaccctgc cttgcaatgg atgtggaact cagtatttat ctcattggta accatgttct
    12661 tagtttgtgc aacctcatct ctagcaggtt atgtattggc taaaaaacgt ttctatggtc
    12721 aacgcattct atttgctatc tttatcgctg ctatggcgct tccaaaacaa gttgtccttg
    12781 taccattggt acgtatcgtc aacttcatgg gaatccacga tactctctgg gcagttatct
    12841 tgcctttgat tggatggcca ttcggtgtct tcctcatgaa acagttcagt gaaaatatcc
    12901 ctacagagtt gcttgaatca gctaaaatcg acggttgtgg tgagattcgt accttctgga
    12961 gtgtagcctt cccgattgtg aaaccagggt ttgcagccct tgcaatcttt accttcatca
    13021 atacttggaa tgactacttc atgcagttgg taatgttgac ttcacgtaac aatttgacca
    13081 tctcacttgg ggttgcgacc atgcaggctg aaatggcaac caactatggt ttgattatgg
    13141 caggagctgc ccttgctgct gttccaatcg tcacagtctt cctagtcttc caaaaatcct
    13201 tcacacaggg tattactatg ggagcggtca aaggataata ctctgcgaaa atcgaatgca
    13261 aactacgtca gcttcacctt gccatactta agtattgcct gtggttagct tcctagtttg
    13321 ttcttcaatt ttcattgagt ataggaaaat caatctatca aaatacagaa gtatatttta
    13381 tagatttaga gaatatagaa gttataagtg tctacaaaat ggagggtatg cagttacttt
    13441 atgaagtttt gtcagacact tatcaactta agaatggttt tagttaacta tcagaaacga
    13501 aggaaagagt atgatttttg acgatttgaa aaacatcacc ttttacaaag ggattcatcc
    13561 caatttagac aaggctatcg actatctcta ccaacatcgt aaagattcat tcgaattagg
    13621 aaagtatgag attgatggag ataaagtctt tctagttgtt caggaaaatg tcctcaatca
    13681 agttgagaat aatcaatttg aacaccataa gaactatgca gatttgcatt tgctggtaga
    13741 aggacatgaa tattcgagct acggttcacg tatcaaagac gaggcagtag cattcgacga
    13801 agcgagtgac attggctttg ttcattgtca tgaacactac ccactcttgt tgggttatca
    13861 caattttgcg attttcttcc caggtgagcc acatcagcca aatggttatg caggcatgga
    13921 agaaaaggtt cgaaaatatc tctttaaaat tttgattgat taaaaatagg atgaattgtt
    13981 tttttgtaaa gctttgacaa tactctacca tgaaattgat ctttgtgagg tagggaaatg
    14041 agaataaaat atttaaaaat tggtatcttc ttgtcagtct gtgtatgcta caagagctag
    14101 tttcttggat ggacagggaa ttacagttga tgagatggct tggataatta ggggcattgt
    14161 gaatgcattg attggtagat acataaaatt aggtacttat gcggctaagt atggtattag
    14221 tatggcgcgt tctatcttaa atagggcagc tgcaactgcg gcagcaagag taggattatt
    14281 gactaagatt tttggatgga ttttacgaat agctgtgctc gcagctgatg tatatggtaa
    14341 ttttgccaac aatattgctg tagcttggga tgcacatgat aaaatcccta acaatggtcg
    14401 tataaacttt taaaatgcga gaatgaaagc actttgtatt tttttattga atatgttagc
    14461 ttggacagtg cttgcaatga taatccgtgg agggctagat ggatttgata ggcatacttg
    14521 gagtactatt ttaattgctt cgctgttcgg ggtatatgat tataagccca tagataaaaa
    14581 tagaaaaaag tccaaaagaa aaaagaaaaa atagatttgt tcatggtagg gacttatgaa
    14641 agctttactg acaaaaaaga aaacagttta caaagaaaaa tgatggagga gcaaacatgg
    14701 cacaaaaagg agtaagcctt atcaaggcag catttgatac agacaacttt ctcatgcgtt
    14761 ttagtgagaa agtcttggac atcgtgacag ccaatcttct ttttgtcgtc tcttgtttgc
    14821 ccatcgtgac gattggagtg gctaaaatca gcctctacga gaccatgttc gaagttaaga
    14881 agagcagacg ggtgcctgtt tttaaaatct atctaagatc tttcaagcaa aatctgaaac
    14941 taggtcttca gctgggttta atggagttag gaattgtgtt tcttaccctt tcagatctct
    15001 atcttttctg gggtcaaaca gctccgccct tccaattgct gaaagccatt tgtttaggta
    15061 ttctgatttt tcttactatc gtgatgctgg ctagttaccc tatcgcggca cgttatgacc
    15121 tatcttggaa agaaattctt caaaaaggat tgatgttggc tagttttaac tttccttggt
    15181 tcttcctcat gttagccatt cttgtcctca ttgtgatggt tctttatctg tccgccttca
    15241 gtctactctt aggtggctca gtcttcttac tttttgggtt tggactattg gtctttatcc
    15301 agactggatt gatggagaaa attttcgcaa aataccaata ggagccttat ttctgaaact
    15361 actttcaaac gctccaaacg ctattctata agcgagaaac taaaatctaa gtgtctataa
    15421 gatattgaaa gccttttcgg taaagtgtat actaaagaag taaagaaaaa tctgctttag
    15481 cagaaaataa aaataatagg agaaaatcta tgtcagattt gaaaaaatac gaaggtgtca
    15541 ttccagcctt ctacgcatgt tatgatgatc aaggagaagt aagcccagaa cgtacgcgtg
    15601 ccttggttca atacttcatt gataaaggtg ttcaaggtct ttatgtcaat ggttcttctg
    15661 gtgaatgtat ctaccaaagc gttgaagatc gcaagttgat tttggaagaa gtcatggcgg
    15721 tagccaaagg taaattgacc atcattgctc atgttgcctg caacaatact aaagatagta
    15781 tggaacttgc tcgccatgct gaaagcttgg gagtagatgc tattgcaacg attccaccaa
    15841 tttatttccg cttgccagaa tactcagttg ccaaatactg gaacgatatc agttctgcag
    15901 ctccaaacac agactacgtg atttacaaca ttcctcaatt ggcaggggtt gctttgactc
    15961 caagccttta cacagaaatg ttgaaaaatc ctcgtgttat cggtgttaag aactcttcta
    16021 tgccagttca agatatccaa acctttgtca gccttggtgg ggaagaccat atcgtcttta
    16081 atggtcctga tgagcagttc ctaggaggac gcctcgtggg tgctagggct ggtatcggtg
    16141 gtacttatgg tgctatgcca gaactcttct tgaaactcaa tcagttgatt gcggataagg
    16201 acctagaaac agcgcgtgaa ttgcagtatg ctatcaacgc aatcattggt aaactcactt
    16261 ctgctcatgg aaatatgtac ggtgtcatta aagaagtctt gaaaatcaat gaaggcttga
    16321 atattggatc tgttcgttca ccattgacac cagtgactga agaagatcgt ccagttgtag
    16381 aagcggctgc tgccttgatt cgtgaaacca aggagcgctt cctctaatct aaaaggaggt
    16441 atttatgaca tactacgttg caattgatat cggtggaacc aacatcaagt atggtttggt
    16501 tgatcaagag gggcaacttc ttgaatcgca tgaaatgcca actgaggcgc ataagggtgg
    16561 acctcatatc ttacaaaaga ccaaagatat cgtagctagt tatttagaaa aaggcccagt
    16621 agcaggtgtt gccatatctt ctgctgggat ggtggatccg gataagggtg agattttcta
    16681 tgctgggcct caaatcccta actacgcagg cacccagttc aaaaaggaaa tcgaagaaag
    16741 ctttactatt ccttgtgaga ttgaaaatga tgtcaactgt gcaggtcttg ctgaggcagt
    16801 atctggttca ggcaagggag caagtgtgac actttgcttg accattggaa ccggtatcgg
    16861 tggttgcttg attatggata ggaaagtctt ccatggtttt agcaattcag cctgtgaagt
    16921 cgggtatatg catatgcagg atggagcttt tcaggatctc gcttctacaa cagctttagt
    16981 gaaatatgta gctgaagccc atggagaaga tgttgatcag tggaatggcc gacgtatctt
    17041 taaggaagct actgaaggaa acaaaatctg catggaaggt attgaccgta tggttgacta
    17101 tctaggaaaa ggtctggcaa atatttgtta tgtggccaat ccagaagtgg ttattcttgg
    17161 tggtggtatc atggggcaag aggctatcct caaacctaag atccgcacag ccttgaaaga
    17221 ggcattggta ccaagtttag cagaaaaaac acgattagaa tttgcccatc accaaaatac
    17281 agcagggatg ttgggtgcat attatcattt taagacaaaa caatcctagt ttggctcagc
    17341 caaactagga ttttcttaca cgtttttgtc tacgatagcc gttgagtttt ttattttccc
    17401 agtagctatt aaagattttt tccttgcttt cgcgattgat ttccaaaaag taggcataaa
    17461 tcaaatcgat aaagaagagc ataggaagtt gagcggatat tcgttggatg taggagggtt
    17521 ggctgtgggt ggctacaaga acagtctctg tataggtctg gctatcttta ttgggaacac
    17581 ttgtaaagag tacagtcttt gcccccatct ccttggcatc taatagacta tctaaaatag
    17641 aaggagttga gcctgaaagt gagaacccca gtactagaca attttcatcc atgatgctgg
    17701 ttgtccaggc aaagccgtct tggtctgtca aagcttcgca gaccacacct agtcgcataa
    17761 aacgtaattt catttcacgg gcgacgaggc cagaactccc tgttccaaag aagtagatac
    17821 gctcagcatc ttcgattagc tgggcaattc gctctagttg gatttcgtca atcaagtctt
    17881 gtgtttgttc cctcatattg ctataacttc tgaggactcg tttggtcagt ggactatgct
    17941 tggagacttg gttggcttga ttttctgcct gatgttggta ttggaaaata aattctcggt
    18001 agccagtaaa gccacacttt ttagcaaagc gggtcaaagc agcttgagaa atatgtaatt
    18061 tttgggtgac ttgttgagaa gataaatcat ctgtaatcgt ttcagcttgc aaaaaatagc
    18121 gagcgatttc ttgctctagg tctgtcattt cttcaaaatg tgaatcaatg atagttgcga
    18181 tatctggttt gtccataggg aaagctcctt tacatgggtc atactggaag actagatcag
    18241 ggaatagtca cacttcatta taacacataa tataaggata gataaataaa aacgcatctc
    18301 tgttttaaaa acgaaaaaat cgaaaaagct tctctctttt ccataatttt ctactcaaat
    18361 tgtggtacaa ttaagagtaa gattttaagt tagaaatgag actgatttgt atgagaaaat
    18421 ttaacagcca ttcgattccg attcggctta atttattgtt ttcaatcgtc attttactct
    18481 ttatgaccat tattggtcgt ttgttgtata tgcaggtttt gaacaaggat ttttacgaaa
    18541 aaaagctagc ctcagctagt cagaccaaga ttacaagcag ttcagcccgt ggggaaattt
    18601 atgatgctag tggaaaacct ttggtagaaa atacgttaaa gcaggttgtt tcctttacgc
    18661 gtagcaataa aatgacggct acagacttaa aagaaacagc taaaaagtta ctgacttatg
    18721 tgagcatcag ttctccaaat ttgacagaac gccagctggc ggattactat ttggctgatc
    18781 ctgaaatcta taaaaaaaca gtggaagctc tcccaagtga gaaacgcttg gattcagatg
    18841 gcaatcgtct atccgaatca gaactgtata acaatgcggt cgatagtgtc caaacaagtc
    18901 aactaaacta tacagaggat gaaaagaaag aaatctatct ttttagtcag ttaaatgctg
    18961 ttggaaactt tgcgacagga accattgcga cagatcctct aaatgattct caggtggctg
    19021 ttattgcctc tatttcaaag gagatgcctg gcattagtat ttctacttct tgggatagaa
    19081 aggttttgga aacttccctt tcttctatag ttgggagtgt atccagtgaa aaagctggtc
    19141 tcccagcgga agaagcagaa gcctatctta aaaaaggcta ttctctaaat gaccgtgttg
    19201 gaacctcgta tttggaaaag caatatgaag agaccttaca aggaaaacgc tcggtaaaag
    19261 aaatccatct ggataaatat ggcaatatgg aaagcgtgga tacaattgag gaaggtagta
    19321 agggaaacaa tatcaagctg accattgatt tggccttcca agatagcgtg gatgctttgc
    19381 tgaaaagtta tttcaattcc gagctaggaa atggtggagc caagtattct gagggtgtgt
    19441 atgcagtcgc ccttaacccc aaaacaggtg ctgttttgtc tatgtcagga ctcaaacatg
    19501 acctgaaaac gggagagttg actcctgatt ccttgggaac ggtaaccaat gtctttgtcc
    19561 caggttcggt tgttaaggcc gctaccatca gctcaggttg ggaaaatggt gttttatcag
    19621 gaaaccaaac cttaacagat cagcctattg ttttccaagg ttcagctcca atttattctt
    19681 ggtataaatt ggcatatgga tcttttccta ttacagctgt ggaagccttg gagtattcat
    19741 ccaatgctta catggttcaa accgctcttg gaatcatggg ccagacctat caaccaaata
    19801 tgtttgttgg aaccagcaat ttggaaacag ctatgggaaa acttcgtgcg acctttggcg
    19861 aatatggctt gggggctgcg accggaattg acctaccaga tgaatctact ggatttgttc
    19921 ccaaagagta tagctttgct aattacatca ccaatgcctt tgggcagttt gataactata
    19981 cgcccatgca gttggctcag tatgtagcaa ctattgcaaa tgatggtgtt cgtgtggctc
    20041 ctcgtattgt tgaaggcatt tatggtaata atgataaggg aggactgggt gacttgattc
    20101 agcaactgca accgacagag atgaataagg tcaatatatc cgactccgat atgagtatct
    20161 tgcaccaagg tttttatcag gttgctcatg ggactagtgg attgacaact ggacgtgctt
    20221 tttcaaatgg tgccttggta tccattagtg ggaaaacagg tactgccgaa agttatgttg
    20281 caggtggtca agaagccaac aataccaacg cggtggccta tgccccatca gataatcctc
    20341 aaatcgctgt tgccgttgtc ttccctcata acaccaatct aacaaatggt gttggacctt
    20401 ctattgcacg cgatattatc aacctctata accaacacca tccaatgaat tagaaaggaa
    20461 cttatgcttt atccaacacc tattgccaag ctaattgata gttattctaa gttaccgggt
    20521 atcgggatta agacggctac gcgtctggcc ttttatacca ttggaatgtc tgatgacgac
    20581 gtcaatgaat ttgccaaaaa tctcctttct gctaagagag agctgaccta ctgttctatt
    20641 tgtggtcgat tgactgatga tgatccttgt tctatctgta ccgatccgac tcgtgaccag
    20701 acaacgattt tggtgctaga ggatagtcgt gatgtggcgg ccatggaaaa tatccaagaa
    20761 taccatggac tttatcatgt cctgcatggt ctcatttctc ccatgaatgg cattagtcca
    20821 gatgatatca atctcaagag cctcatgacc cgtcttatgg atagtgaggt ttcagaagtg
    20881 attgtagcca caaatgccac agcagatggg gaagcgactt cgatgtacct ttcccgcctg
    20941 ctcaagccag ctgggataaa ggttactcgt ctggcgcgag gcctcgctgt gggagcggat
    21001 atcgagtatg cggacgaagt gaccctctta cgagctattg aaaatcggac agagttgtaa
    21061 gtctagacaa attaacaaac tcaaattcat ttatacaaaa atcacggaga ccgacagttg
    21121 ttttatcggt ctctttttag atttcttgaa caccagtatc aggttcaagt ttcaaaaaag
    21181 aagcaaatat gatatactaa agaacgagta ttctattaga attggaacaa ataatatgaa
    21241 acaaacgatt attcttctat acggtggacg tagtgcagag cgcgaagtct ctgttctttc
    21301 agcggaaagt gtgatgcgtg cggtcaatta cgatcgtttc acagtcaaga ctttctttat
    21361 cagccagtca ggtgacttta tcaaaacgca ggaatttagt cagactccag gtcaagagga
    21421 ccgtctcatg accaatgcga ccattgattg ggataagcag gttgcaccaa gtgccatcta
    21481 cgaagaaggc gcagtggtct ttccagttct tcatggtccg atgggagaag atggctctgt
    21541 tcaaggattc ttggaagttt tgaaaatgcc ttatgtcggc tgcaacatct tgtcatccag
    21601 tcttgccatg gataaaatca cgactaaacg tgttttggaa tctgctggta ttgcccaagt
    21661 tccttatgtg gccatcgttg aaggtgatga tgtgactgct aaaattgctg aagtggaaga
    21721 aaaattgact tatccagtct tcaccaaacc atcaaacatg ggttcaagtg tcggtatttc
    21781 taagtctgaa aatcaagaag aactccgtca agctttgaaa cttgccttcc aatatgacag
    21841 ccgtgtcttg gttgagcaag gggtgaatgc ccgtgaaatc gaggttggtc tcttgggcaa
    21901 ctacgatgtc aaaagcacgc tgccaggaga agttgtcaaa gatgttgcct tttatgacta
    21961 tgacgccaag tatattgata acaagattac tatggatatt ccagccaaga tcagtgatga
    22021 tgtagtagct gtcatgcgtc gaaatgcaga aacggccttc cgtgcgattg gtggactagg
    22081 tctctctcgt tgcgatttct tctatacaga caagggcgag attttcctaa acgagctaaa
    22141 taccatgcca ggtttcaccc agtggtctat gtacccactg ctttgggata atatgggaat
    22201 cagctaccca gacctaatcg agcatttggt tgaccttgcc aaggaaagtt ttgacaagcg
    22261 cgaagcgcat ttgctataaa aatgaaagag agggtagaag tcagaaccat cactgccagg
    22321 tgatcagagt tctcggactt caaccctttt taaaggagta gaaatgaaac ttacaattca
    22381 cgaagttgcc caagctgttg gagctaaaaa taatgtcagc atctttgagg acatccagtt
    22441 agaaaaggtt gagtttgaca gtcgtttgat tgggactgga gatttatttg tgccactcaa
    22501 gggagcgcgt gatggtcatg actttatcga aacagcattt gaaaatggtg cagcagtaac
    22561 cttgtctgag aaagaggtcg caaatcatcc ttatatttta gtagacgatg tgttgactgc
    22621 ctttcaacaa ctagctgcct actatcttga gaaaactgct gttgatgtct ttgcagttac
    22681 aggttcaaat ggtaagacga cgaccaagga tatgttggcg cacttgcttt caacaaccta
    22741 taaaacctac aaaacacaag gaaactacaa taacgagatt ggccttcctt acacagttct
    22801 acacatgcct gaggggacag aaaagttggt tttggagatg gggcaggatc acttgggaga
    22861 tattcatctc ttgtcagaat tggcccatcc aaaaacagcc attgtgacct tggttggaga
    22921 ggctcatttg gcctttttca aagatcgttc ggagatcgct aaaggaaaaa tgcaaattgc
    22981 agatggtatg gcaccaggtt ctttgctttt ggcaccagct gactcgattg tagaggccta
    23041 tttgcctaca gataaaaaag tggtccgttt tgggcaagga gcagagttgg aaatcacaga
    23101 cttggttgag cgcaaggata gtctgacctt taaggctaat ttcttggagc aagcccttga
    23161 cttgccagtg actggtaagt acaatgccac taatgctatg attgcgtctt atgttgccct
    23221 acaagaagga gtatcagagg agcaaattcg tcaggcctta caaaatcttg aattgacgcg
    23281 taaccgtacc gagtggaaga aagcagccaa tggagcagat attttgtctg atgtatacaa
    23341 tgccaatccg actgctatga agctgatttt ggagactttc tctgccattc cagctaatga
    23401 agggggcaag aaaatcgctg tcttggcaga catgaaggaa ctcggtgacc agtctgttca
    23461 gctccataac cagatgattt tgagtctctc gccagatgtg ttggataccg ttattttcta
    23521 cggagaagac attgccgaat tggcccaact ggctagtcaa atgttcccaa ttggccacgt
    23581 ttactacttc aagaaaacag atgaccaaga ccaatttgaa gacctagtca agcaggtcaa
    23641 ggaaagcctt ggggctcatg atcaaatcct gctcaaaggc tctaactcta tgaacctagc
    23701 taagctggta gaaagtttag aacatgaaga caagtgattt tgtcaagtat ttgcaaagaa
    23761 tgattgccat tacagatact ggattaacct ttacaaagga tccgtttgac cgtgagcgct
    23821 acgaagactt gcgaggcctt ttatctgaaa tgttgaatca agcatcagac cttgatgccg
    23881 aagaagtggc agaagtcttg aatccaactt ctgcttatgc gactccgtta atggacgtcc
    23941 gtgcttggat tgttgaggag gaaaagattt gtctggttag gggacaagga gagaatgatt
    24001 gggctttgcc aggtggcttt ggtgaggttg gctattctcc aaccgaaaat attctcaagg
    24061 aaattgaaga agaaaccggt tttaaagcca aagttgaaag actgctagct gtttttgata
    24121 ccaatcgttt ccaactacag agcaaacaat atgcaaagtt tgtttttgaa tgtaagtttc
    24181 tcgatggaca atttcaagaa aatcaagaaa tcgctgacct tcaatttttt gccattgacc
    24241 agctgccgaa cttgtctgaa aaacgcatta ccaaggagca aatcaagatt ctttggcagg
    24301 tttatcaagg tcagagggag caatatcttg actaagaaga tgattatcgt atttctaaat
    24361 ccatttttga caactagcat ggtataataa tatgcaggaa aattttgaat catgaggaag
    24421 actagatgaa tttatgggat attttcttta cgactcaggc aaccgagccg cccaaatttg
    24481 accttttttg gtatgttagc ctatttacgc tcttagcctt aaccttttat acagcccatc
    24541 gctatcgtga aaagaaggtt taccaacgat ttttccaaat cttgcagact gttcagttaa
    24601 tccttcttta tggttggtac tgggtcaatc atatgccgct gtcagaaagc ctaccctttt
    24661 accattgccg tatggctatg tttgtggtac tcttgcttcc tggtcagtcc aaatataaac
    24721 aatactttgc attattggga acatttggga cattagcagc ctttgtttat ccagtgccag
    24781 atgcttaccc ttgtccacat atcaccattc tatcctttat ctttggtcat ttagcactct
    24841 tggggaactc tctagtttat ctattgagac agtataatgc gcgattgctg gatgtgaagg
    24901 gaatttttct catgaccttt gccctaaatg ccttgatttt tgtggtcaat ttggtgacag
    24961 gtggcgatta cggatttttg acaaaaccgc cattggttgg ggatcacggt ctagtagcta
    25021 attatttact tgtttcaatt gtgctggtag ctactatcag tttgactaag aaaatcttag
    25081 aattcttttt agctcaagaa gcagaaaaaa tgattgcaaa ggaagcttaa cacagagctt
    25141 tcttttttgc tcttagagag tttttacaag cagcttataa aataagaatt tctgaataga
    25201 caaactcaaa aaatggctgg gaaatttagg aaaaaagcaa gcacgattaa attttttgtg
    25261 ttataatatt ttgtgaatag ctatgcctat gtttagctat ggaataatac gaagtgcgaa
    25321 acttggaaga tagagaggaa gcgatgtaat ggctagagaa ggctttttta caggtctaga
    25381 tattggaaca agctctgtca aggtgcttgt ggccgagcag agaaatggtg aattaaatgt
    25441 aattggcgtg agtaatgcca aaagtaaagg tgtaaaggat ggaattattg ttgatattga
    25501 tgcagcagca actgctatca agtcagccat ttcccaagcg gaagaaaagg caggcatttc
    25561 gattaaatca gtaaatgtcg gcttgcctgg taatcttttg caggtagaac caactcaggg
    25621 gatgattcca gtaacatctg atactaagga aattacggat caagatgttg aaaatgttgt
    25681 caaatcagct ttgacaaaga gtatgacacc tgaccgtgaa gtcattacct ttattcctga
    25741 agaatttatt gtggatggtt tccaagggat tcgtgaccca cgtggcatga tgggggttcg
    25801 ccttgaaatg cgtggtttgc tttatacagg acctcgtact atcttgcaca atttgcgtaa
    25861 gacggttgag cgtgcaggtg ttcaggttga aaatgttatc atttcaccac tagcaatggt
    25921 tcagtctgtt ttgaacgaag gggaacgtga atttggtgct acagtgattg atatgggggc
    25981 aggtcaaacg actgtcgcta caatccgtaa tcaagaactc cagttcacac atattctcca
    26041 agaaggtgga gattatgtaa ctaaagatat ctccaaggtt ttgaaaacct ctcgcaaatt
    26101 agcggaaggc ttgaaactga attacgggga agcctatccg cctcttgcaa gcaaagaaac
    26161 cttccaagta gaggttattg gagaagtaga agcagtcgaa gtgacggaag cctacttgtc
    26221 agaaattatt tctgcacgaa tcaagcacat ccttgaacaa atcaagcaag aattagatag
    26281 aaggcgtcta ttggacctcc ctggtggtat tgtcttaatc ggtgggaatg ccattttacc
    26341 aggtatggtt gagcttgctc aggaagtctt tggcgtccgt gtcaagcttt atgttccaaa
    26401 tcaagttggt atccgtaatc cagcctttgc gcatgtgatt agtttatcag aatttgcggg
    26461 tcaattaaca gaagttaatc ttttggctca gggagcgata aaaggtgaga atgacttaag
    26521 tcatcagcca attagttttg gtgggatgct gcaaaaaaca gctcagtttg tacaatcaac
    26581 gcctgttcaa ccagctcctg ctccagaagt agagtcggtg gcgcctacag aaccaatggc
    26641 ggatttccaa caagcttcac aaaataaacc gaaattagca gatcgtttcc gtggcttgat
    26701 cggaagcatg tttgacgaat aaagaggaaa aataaattat gacattttca tttgatacag
    26761 ctgctgctca aggggcagtg attaaagtaa ttggtgtcgg tggaggtggt ggcaatgcca
    26821 tcaaccgtat ggtcgacgaa ggtgttacag gcgtagaatt tatcgcagca aacacagatg
    26881 tacaagcatt gagtagtaca aaagctgaga ctgttattca gttggggcct aaattgactc
    26941 gtggtttggg tgcaggaggt caacctgagg ttggtcgtaa agccgctgaa gaaagcgaag
    27001 aaacactgac ggaagctatt agtggtgccg atatggtctt catcactgct ggtatgggag
    27061 gaggctctgg aactggagct gctcctgtta ttgctcgtat cgccaaagat ttaggtgcgc
    27121 ttacagttgg tgttgtaaca cgtccctttg gttttgaagg aagtaagcgt ggacaatttg
    27181 ctgtagaagg aatcaatcaa cttcgtgagc atgtagacac tctattgatt atctcaaaca
    27241 acaatttgct tgaaattgtt gataagaaaa caccgctttt ggaggctctt agcgaagcgg
    27301 ataacgttct tcgtcaaggt gttcaaggga ttaccgattt gattaccaat ccaggattga
    27361 ttaaccttga ctttgccgat gtgaaaacgg taatggcaaa caaagggaat gctcttatgg
    27421 gtattggtat cggtagtgga gaagaacgtg tggtagaagc ggcacgtaag gcaatctatt
    27481 caccacttct tgaaacaact attgacggtg ctgaggatgt tatcgtcaac gttactggtg
    27541 gtcttgactt aaccttgatt gaggcagaag aggcttcaca aattgtgaac caggcagcag
    27601 gtcaaggagt gaacatctgg ctcggtactt caattgatga aagtatgcgt gatgaaattc
    27661 gtgtaacagt tgttgcaacg ggtgttcgtc aagaccgcgt agaaaaggtt gtgtctccac
    27721 aagctagaac tgctactaac taccgtgaga cagtgaaacc agctcattca catggctttg
    27781 atcgtcattt tgatatggca gaaacagttg aattgccaaa acaaaatcca cgtcgtttgg
    27841 aaccaactca ggcatctgct tttggtgatt gggatcttcg ccgtgaatcg attgttcgta
    27901 caacagattc agtcgtttct ccagtcgagc gctttgaagc cccaatttca caagatgaag
    27961 atgaattgga tacacctcca tttttcaaaa atcgttaagt aaatgaatgt aaaagaaaat
    28021 acagaacttg tttttcgaga agttgcagag gctagtctga gtgctcatcg agagagtggt
    28081 tcggtctctg tcattgcagt taccaagtat gtagatgtac cgacagcgga agccttgctt
    28141 ccgctaggtg ttcatcatat cggtgaaaat cgtgtagata agtttctgga aaaatatgaa
    28201 gctttaaaag atcgagatgt gacttggcat ttgattggta ccttgcaaag acgtaaggtg
    28261 aaagatgtca ttcaatacgt tgattatttc catgcattgg actcagtaaa gctagcaggg
    28321 gaaattcaaa aaagaagtga ccgagtcatc aagtgtttcc ttcaagtaaa tatttctaaa
    28381 gaagaaagca aacacggttt ttcgagagag gaactgctgg aaatcttgcc agagttagcc
    28441 agactagata agattgaata tgttggttta atgacgatgg caccttttga ggctagcagt
    28501 gagcagttga aagagatttt caaggcggcc caagatttac aaagagaaat tcaagagaaa
    28561 caaattccaa atatgcctat gaccgagtta agtatgggaa tgagtcgtga ttataaagaa
    28621 gcgattcaat tcggttccac ttttgttcgt ataggtacat cattttttaa gtaggagaga
    28681 accatgtctt taaaagatag attcgataga tttatagatt attttacgga ggatgaggat
    28741 tcaagtctcc cttatgaaaa aagagatgag cctgtgttta ctccagtaaa ttcttcacag
    28801 gaaccggctc tcccaatgaa tcaaccttca cagtcggctg gtacaaaaga gaacaatatc
    28861 accagacttc atgcaagaca acaggaattg gcaaatcaga gtcagcgtgc aacggataag
    28921 gtcattatag atgttcgtta tcctagaaaa tatgaggatg caacagaaat tgttgattta
    28981 ttggcaggaa acgaaagtat cttgattgat tttcagtata tgacagaggt gcaggctcgt
    29041 cgttgtttgg actatttgga tggagcttgt catgttttag ctggaaattt gaaaaaggta
    29101 gcttctacca tgtatttgtt gacaccagtg aatgttattg taaatgttga agatatccgt
    29161 ttaccagatg aagatcaaca gggtgagttc ggttttgata tgaagcgaaa tagagtacga
    29221 taatgatttt tttaattcgt atgatttata atgcagtgga tatttactcc ctgattttgg
    29281 tagccttcgc tgtcatgtct tggtttccag gtgcctacga atccagttta ggtcgttgga
    29341 ttgtagcgtt ggtgaaacca gtgcttgctc ccttgcaacg cctgccttta cagatagcgg
    29401 gtcttgattt atctgtttgg gttgcgattg ttttggttcg atttttagga gaaaacctag
    29461 tgcgttttct ggcgatgata ggatgaataa agggatttat cagcatttct ccatagaaga
    29521 tcgtccattt cttgacaagg gaatggaatg gataaagaag gtagaagata gctatgctcc
    29581 ttttttaact ccttttatca atcctcatca ggagaagcta ttaaagattt tggccaaaac
    29641 ctatggtctt gcttgtagca gtagtgggga attcgtctcg agtgagtatg ttcgagtttt
    29701 attataccca gattatttcc aaccagagtt ttcagatttt gaaatatctc tccaggaaat
    29761 tgtgtattcc aataaatttg aatatttaac gcatgctaag attttaggga cagtcatcaa
    29821 tcaattaggg attgaacgga aactttttgg agatatccta gtagatgaag aacgggcgca
    29881 gattatgatt aatcagcagt ttcttcttct cttttaagat ggactaaaga aaattggtcg
    29941 tatacctgtt tcgctggagg aacgtccttt caccgagaaa atagataagc tagaacagta
    30001 tcgagaactg gatttatctg tgtctagttt tcgattagat gttcttttat caaatgtttt
    30061 gaaactatct aggaatcaag caaaccagtt gattgaaaag aaacttgtcc aagtaaatta
    30121 tcatgtggta gacaaatcag attacactgt tcaagttgga gacttgatta gtgtgagaaa
    30181 atttggtcgc ttgagattac ttcaagataa gggacaaacg aaaaaagaga agaaaaaaat
    30241 aaccgtccag ttattattaa gtaagtgagg aatagaatgc caattacatc attagaaata
    30301 aaggacaaga cttttggaac tcgattcaga ggttttgatc cagaagaagt cgatgaattt
    30361 ttagatattg tggttcgtga ttacgaagat cttgtgcgtg cgaatcatga taaaaatttg
    30421 cgtattaaga gtttagaaga gcgtttgtct tactttgatg aaataaaaga ttcattgagc
    30481 cagtctgtat tgattgccca ggatacagct gagcgagtga aacaggcggc gcatgaacgt
    30541 tcaaacaata tcattcatca agcagagcaa gatgcgcaac gcttgttgga agaagctaaa
    30601 tataaggcaa acgagattct tcgtcaagca actgataatg ctaagaaagt cgctgttgaa
    30661 acagaagaat tgaagaacaa gagccgtgtc ttccaccaac gtctcaaatc tacaattgag
    30721 agtcagttgg ctattgttga atcttcagat tgggaagata ttctccgtcc aacagctact
    30781 tatcttcaaa ccagtgatga agcctttaaa gaagtggtta gcgaagtact tggagaaccg
    30841 attccagttc caattgaaga agaaccaatt gatatgacac gtcagttctc tcaagcagaa
    30901 atggaagaat tacaagctcg tattgaggta gccgataaag aattgtctga atttgaagct
    30961 cagattaaac aggaagtgga agctccaact cctgtagtga gtcctcaagt tgaagaagag
    31021 cctctgctca tccagttggc ccaatgtatg aagaaccaga agtagctcca gtgcatccga
    31081 caggtccaac accagctaca gaaactgttg attcagcacc gggatttgaa gcaccgcaag
    31141 aatctgttac aattttataa gaaatattct gagaacaata tcttatcctt atattcccag
    31201 cgagcaggag atggtgtgag tcctgcattc cctatcgata agattatcct ctcaaaaact
    31261 caagtctgaa tctagtaaga tttgacgttc cccacgttac gggataagag ggagaaagac
    31321 taaaactttt tccgaataaa ggtggtacca cgattttcgt cctttttgga agtcgtggtt
    31381 tttaatttgt tattatttat aaaggagata ccatgaaact caaagacacc cttaatcttg
    31441 ggaaaactga attcccaatg cgtgcaggcc ttcctaccaa agagccagtt tggcaaaagg
    31501 aatgggaaga tgcaaaactt tatcaacgtc gtcaagaatt gaaccaagga aaacctcatt
    31561 tcaccttgca tgatggccct ccatacgcta acggaaatat ccacgttgga catgctatga
    31621 acaagatttc aaaagatatt attgttcgtt ctaagtctat gtcaggattt tacgcaccat
    31681 ttattcctgg ttgggatact catggtctgc caatcgagca agtattgtca aaacaaggtg
    31741 tcaaacgtaa agaaatggac ttggttgagt acttgaaact ttgccgtgag tacgctcttt
    31801 ctcaagtaga taaacaacgt gaagatttta aacgtttggg tgtttctggt gactgggaaa
    31861 atccatatgt gaccttgact cctgactatg aagcagctca aattcgtgta tttggtgaga
    31921 tggctaataa gggttatatc taccgtggtg ccaagccagt ttactggtca tggtcatctg
    31981 agtcagccct tgctgaagca gagattgaat accatgactt ggtttcaact tccctttact
    32041 atgccaacaa ggtaaaagat ggcaaaggag ttctagatac agatacttat atcgttgtct
    32101 ggacaacgac tccatttacc atcacagctt ctcgtggttt gacggttggt gcagatattg
    32161 attacgtttt ggttcaacct gctggtgaag ctcgtaagtt tgtcgttgct gctgaattat
    32221 tgactagctt gtctgagaaa tttggctggg ctgatgttca agttttggaa acttaccgtg
    32281 gccaagaact caaccacatc gtaacagaac acccatggga tacagctgta gaagagttgg
    32341 taattcttgg tgaccacgtt acgactgact ctggtacagg tattgtccat acagcccctg
    32401 gttttggtga ggacgattac aatgttggta ttgctaataa tcttgaagtc gcagtgactg
    32461 ttgatgaacg tggtatcatg atgaagaatg ctggtcctga atttgaaggt caattctatg
    32521 aaaaggtagt tccaactgtt attgaaaaac ttggtaacct ccttcttgcc caagaagaaa
    32581 tctctcactc atatccattt gactggcgta ctaagaaacc aatcatttgg cgtgcagttc
    32641 cacaatggtt tgcctcagtt tctaaattcc gtcaagaaat cttggacgaa attgaaaaag
    32701 tgaaattcca ctcagaatgg ggtaaagtcc gtctttacaa tatgatccgt gaccgtggtg
    32761 actgggttat ctctcgtcaa cgtgcttggg gtgttccact tcctatcttc tacgctgaag
    32821 atggtacagc tatcatggta gctgaaacta ttgaacacgt agctcaactt tttgaagaac
    32881 atggttcaag catttggtgg gaacgtgatg ccaaagacct cttgccagaa ggatttactc
    32941 atccaggttc accaaacggc gagttcaaaa aagaaactga tatcatggac gtttggtttg
    33001 actcaggttc atcatggaat ggagtggtgg taaaccgtcc tgaattgact tacccagccg
    33061 acctttacct agaaggttct gaccaatacc gtggttggtt taactcatca cttatcacat
    33121 ctgttgccaa ccatggcgta gcaccttaca aacaaatctt gtcacaaggt tttgcccttg
    33181 atggtaaagg tgagaagatg tctaaatctc ttggaaatac cattgctcca agcgatgttg
    33241 aaaaacaatt cggtgctgaa atcttgcgtc tctgggtaac aagtgttgac tcaagcaatg
    33301 acgtgcgtat ctctatggat atcttgagcc aagtttctga aacttaccgt aagattcgta
    33361 acactcttcg tttcttgatt gccaatacat ctgactttaa cccagctcaa gatacagtcg
    33421 cttacgatga gcttcgttca gttgataagt acatgacgat tcgctttaac cagcttgtca
    33481 agaccattcg tgatgcctat gcagactttg aattcttgac gatctacaag gccttggtga
    33541 actttatcaa cgttgacttg tcagccttct accttgattt tgccaaagat gttgtttaca
    33601 ttgaaggtgc caaatcactg gaacgccgtc aaatgcagac tgtcttctat gacattcttg
    33661 tcaaaatcac caaactcttg acaccaatcc ttcctcacac tgcggaagaa atttggtcat
    33721 atcttgagtt tgaaacagaa gacttcgtcc aattgtcaga attaccagag gctcaaactt
    33781 ttgctaatca agaagaaatc ttggatacat gggcagcctt catggacttc cgtggacaag
    33841 ctcaaaaagc cttggaagaa gctcgtaatg caaaagtaat cggtaaatca cttgaagcac
    33901 acttgacagt ttatccaaac gaagttgtga aaactctact cgaagcagta aacagcaatg
    33961 tggctcaact tctgatcgtg tcagacttga ccatcgcaga aggaccagct ccagaagctg
    34021 cccttagctt cgaagatgta gccttcacag ttgaacgcgc tgcaggtgaa gtatgtgacc
    34081 gttgccgtcg tattgaccca acaacagcag aacgtagcta ccaggcagtt atctgtgacc
    34141 actgtgcaag catcgtagaa gaaaactttg cggatgcagt cgcagaagga tttgaagaga
    34201 aataagattg aaaagtctag gcaaaattca atttgagaag aaacgacaac taattttata
    34261 gtctattaaa cgcattgtat cacgtttttg aacacctgat atgatgcgtt tttatttatt
    34321 ttgaaaattt gtgaggtgtg actttttata ctcaacaaga atcaaagaga aacttagcaa
    34381 gctaacagta gtaagataaa ataggaattt gatattaggg ataagattgg taaatagtgt
    34441 aatattttta caacaataaa tttatatagt tatttctggt ttctgaaaag tattatatta
    34501 tattttattt catattatac aaatttttat tttataatat cagaaaatac tttttttaaa
    34561 aagcaaatat gatacaattt tatttgaaaa aaaataaaaa aggagatttt attatgaaat
    34621 caagtggtat cgtcggtctt gtgggaggaa ttttacttct tatttgtcct tttgtcttgt
    34681 tgggaacagc tgtaaataca gctgctacaa ctcttaatgg aggagctact gcaggggctt
    34741 tttcaggtgt tgccttactc ttgaatgcct tgaagattgc aaatcttgtt cttggtatca
    34801 ttgctattgt ttactataaa ggagataagc gtgtaggtgc agctccgtct gtactaatga
    34861 ttgtttctgg tgaagttagt ctcattccat tcttaggatg ggttgggggg attcttgcta
    34921 ttatcggagg atctctattc cttgcaacat tgaagaaatt caaatcagaa gaataaaagg
    34981 tattttagca tgaaaagaac aaaaaagttt atcggtatag gagtagctct attatctctt
    35041 tctcttctag ttgcatgtgg aacataaagt tcaaagaata cttcaacaag taatgatgag
    35101 aagacagtag caacatccaa tagttcaaaa gaaacaatca ctttcgatac accggttgta
    35161 acagacgatg cgattgaatc aatacgcact tatgcagatt atatagatct ttataaaaat
    35221 atttttgatg attattttac taaaactgag gaaggtttca aaggcacagc tatggaaaat
    35281 aatgactcgt ttactaaact aaaagagtca actcaaaaat tattcgatgc gcagaaaaaa
    35341 aggttaaata atgaagatag aatagaaaca accaaaaaca atgtgattgc caaacattgt
    35401 caaacagtcc tttccttttt ggttttgact agcttttttg tgaaaaattg tgtaaaatag
    35461 aatagataaa cgaggggaaa cctcggaaaa tttaaaggag aatccatcta atggtaaaat
    35521 tggtttttgc tcgccacggt gagtctgaat ggaacaaagc taaccttttc actggttggg
    35581 ctgatgttga tttgtctgaa aaaggtacac aacaagcgat tgacgctggt aaattgatca
    35641 aagaagctgg tatcaaattt gaccaagctt acacttcagt attgaaacgt gctatcaaaa
    35701 caactaactt ggctcttgaa gcttctgacc aattgtgggt tccagttgaa aaatcatggc
    35761 gcttgaacga acgtcactac ggtggtttga ctggtaaaaa taaagctgaa gctgctgaac
    35821 aatttggtga tgagcaagtt cacatctggc gtcgttcata cgatgtattg cctccaaaca
    35881 tggaccgtga tgatgagcac tcagctcaca cagaccgtcg ttacgcttca cttgacgact
    35941 cagttatccc agatgctgaa aacttgaaag tgactttgga acgtgctctt ccattctggg
    36001 aagataaaat cgctccagct cttaaagatg gtaaaaacgt attcgtagga gctcacggta
    36061 actcaatccg tgcccttgta aaacacatca aaggtttgtc agatgacgag atcatggacg
    36121 tggaaatccc taacttccca ccattggtat tcgaattcga cgaaaaattg aacgtcgttt
    36181 ctgaatacta ccttggaaaa taaaaaattg taagtctaga attgatttct aggcttttta
    36241 tgttagtatg gaagtatgat aaggaataaa aaacaagatt atgtactggc ctacaagcaa
    36301 ccagcttcaa ccacttacat gggttgggaa gaagaagctt taccgatagg caatggttct
    36361 ttaggagcaa aagtatttgg ccttataggg gctgaacgga ttcaatttaa tgaaaaaagt
    36421 ctctggtctg gaggtccact tcctgatagt tcagattatc agggtggaaa tcttcaggat
    36481 cagtatgttt ttttagctga gattcggcag gctttggaga agagagatta caatctggct
    36541 aaggaactgg ctgagcagca cctaattggg ccaaaaacga gtcaatatgg gacctatctg
    36601 tcttttgggg atattcacat tgagttcagc cagcaaggta cgactttgtc tcaggtgacg
    36661 gactatcaga gacagctgaa tattagtaag gcacttgcga cgacttctta tgtctataag
    36721 ggaacgcgat ttgaacgtga agcttttgcg agttttccag atgatctctt ggttcaatgt
    36781 tttactaagg aagggttgga aactctagat tttactatag aactatcctt gacctgtgat
    36841 ttggcttcta atggaaagta tgagcaggaa aaatctgatt acaaggagtg taagttggat
    36901 attactgatt ctcatatctt gatgaaggga agagttaagg ataatgatct gcggtttgct
    36961 agttatctag cttgggaaac ggatggagat attagagttt ggtcagatag ggttcagata
    37021 tcaggagcca gttatgccaa tctcttcttg gccgctaaga cggattttgc ccaaaatcct
    37081 gctagcaatt atcgcaagaa actagattta gagcaacagg tgatagactt ggtggacaca
    37141 gctaaagaaa agggctatac ccaattgaaa tcaaggcata tcgaggacta ccaagcctta
    37201 ttccagcgtg ttcaattgga tttggaagct gatgttgacg catccactac agatgatttg
    37261 ttaaaaaatt ataagccaca agaagggcag gctttggagg agctgttctt ccagtatgga
    37321 cggtatttat tgattagttc gtccagagac tgcccagatg ctctaccagc taacctacag
    37381 ggagtctgga atgcggtcga caatcctcct tggaattcgg actatcactt aaatgtcaat
    37441 ctgcagatga attattggcc agcctatgtt accaatctcc tagaggcggt ctttccggtc
    37501 atcaactatg tagatgattt gcgtgtctat ggtcgtctag cggctgtaaa gtatgcagga
    37561 atcgtctctc aaaaaggtga ggaaaatggt tggttggttc atactcaagc gactcccttt
    37621 ggttggacgg cacctggttg ggattactat tggggttggt caccagctgc caatgcgtgg
    37681 atgatgcaaa ccgtttatga agcctattca ttttataggg accaagacta tctcagagag
    37741 aaaatttatc ccatgttgag ggaaacggtt cgtttttgga atgccttttt acataaggat
    37801 cagcaggcgc agcgttgggt gtcttctccg tcttattccc cagaacatgg gccgatttcg
    37861 attggcaata cttatgacca atctctgatt tggcagttat ttcatgattt tattcaggct
    37921 gctcaggaat tgggactgga tgaggacttg ttgactgagg ttaaggagaa gtctgattta
    37981 ctaaatcctt tgcaaatcac tcaatctggt cgaatcaggg agtggtatga ggaggaagag
    38041 cagtattttc aaaatgagaa agtggaggcc cagcatcggc acgcttccca tctagtggga
    38101 ctctatcctg gcaatctctt tagctacagg ggacaagagt atattgaagc ggcacgtgct
    38161 agcctcaatg atcgtgaaga tggcggcaca ggctggtcca aggctaataa gatcaatctc
    38221 tgggcgcgtt tgggagatgg caatcgagcc cataaattat tggcagagca gttaaaaaca
    38281 tccaccttgc caaatctttg gtgtagccat cctccttttc agatagatgg taattttggt
    38341 gctactagtg ccatggcaga aatgttactc cagtctcatg cagcttatct ggtacctcta
    38401 gctgccctac ctgatgcttg gtcaacaggt tctgtttcag gcttaatggc acgtggacat
    38461 tttgaagtga gcatgagctg ggaagataaa aaactcttac agttgaccat tttatcaagg
    38521 agtggaggag atttgcgagt ttcttatcca gatattgaga agagtgtgat taaaatgaat
    38581 caagaaaaaa taaaagcgaa atgcatgggg aaagattgta tttcggtggc aacagcagaa
    38641 ggtgatcttg ttcaatttta tttttaagaa gatgttataa ggcagtaatt tgaaactgcc
    38701 ttttaataag gatttaagaa tataagcagt tttcaactag ttgaaaaagc gttataatga
    38761 taataggaag taatactcaa tgaaaatcaa agagcacaaa ctaggaagct agccgcaggt
    38821 tgctcaaaac agtgttttga ggttgcagat ggaagctgac gtggtttgaa gagagagttt
    38881 cgaggagtat aatttgtttg atagagggtg ggtctgatgg cttatattga gatgaaacac
    38941 tgttacaagc gttatcaggt tggggacacg gagattgtgg ccaatcgtga tgtgaatttt
    39001 gagattgaaa agggggagct ggttattatc cttggtgctt caggtgcagg caagtcaaca
    39061 gttcttaacc ttcttggggg aatggatacc aatgatgaag gggaaatctg gattgatggt
    39121 gttaatattg cggattatag ttcccaccag cgcaccaatt accgtagaaa tgatgtgggg
    39181 tttgtttttc agttttataa tctagtttct aatctgacag ctaaggaaaa tgtggaactg
    39241 gcttctgaaa ttgtgacaga tgccttgaat tctgatcagg tcttgacaga tgtaggtctg
    39301 gctcatcgtc taaataactt tccagcccag ctttctggag gggagcaaca gcgagtctcc
    39361 attgcacgcg cggtagccaa aaatcctaaa attcttcttt gtgatgaacc tactggagcc
    39421 ttggattatc agacgggcaa gcaggttttg aaaattctcc aagatatgtc tcgtcaaaag
    39481 ggagcgacgg tgatcatcgt gactcataat ggagctttgg cgcccattgc tgatcgcgtg
    39541 attcatatgc acgatgccag tgtcaaggat gtggtgctca accagcatcc tcaggatatt
    39601 gacagtttgg agtactagta tgatcaagcg aaaaacttat tggaaggact taattcagtc
    39661 cttcacaggc tccaaggggc gttttttatc catcttgatc ctgatgatgt tgggatctct
    39721 agccttagta ggcctcaaag taaccagtcc caacatggag gcgacagcta atgcttattt
    39781 aacaactgct caaaccttgg atttggcagt catgtctaac tatggcttgg atcaagcaga
    39841 gcaagaagaa ctaaaacaga cggagggcgc agaggtcgag tttggctatt tgacagatgt
    39901 gactatggat aatgggcagg atgccattcg gctgtactcc aaaccagagc gaatttcaac
    39961 ctttcagcta agaaagggac gacttcctca gtcagacaag gaaatcgctt tggccactca
    40021 tttgcaaggc caatacagcg tgggacagga gattagtttt aaagaaaaag aagagggtca
    40081 ttcctcttta aaagaccata cttataccat tactggtttt gtggattcgg ctgaaatcct
    40141 ctcccagcga gatatgggct acgcaggaag tggaagtggg actctgacag cctatggggt
    40201 gattttacct agtcaatttg atcagaaagt ctacaatata gctcgtttga aatatcaaga
    40261 tttagcaggt ttaaatgcct tttcatcagc ttatgaagaa aaatctaagc aacatcagga
    40321 agagcttgaa caaattttat cagataatgg caaggtacgt ctgcaacttt tgaaaaaaga
    40381 aggacaagag tctctagaca aggggcaaga gacccttgac aaggctcaga ctaatttgca
    40441 ggaaggcaag cgtcgtttag cagctgctca agctcgtata caggctcaag aaagtcaact
    40501 agccttgttt cctcaagttc agagagagca ggctagtgct caacttaccc aagccaagca
    40561 ggaattgggc aaggaagagg acaaactaaa gcaagctgaa caaaatctag cccaagaaaa
    40621 ggaaaaatta gaaaaacatc agcaagtctt ggacgatttg gcggagccaa ggtatcaggt
    40681 ctataatcgt cagaccatgc caggtggtca gggctatctt atgtatagca atgcttcatc
    40741 cagtattcga gcagtgggca atatctttcc tgtggtactt tatgccgtag cagccatggt
    40801 gacctttacg accatgactc gctttgtaga cgaagagcga actcatgcag ggatttttaa
    40861 ggccttgggt taccgtagta aggatattat cgccaagttt ctcctttatg gactagtagc
    40921 tgggactgtc ggaacggctc taggtagtat acttggtcat tatttgctag ccagtgtaat
    40981 ttcaagtgtc attacaaaag gcatggtggt gggagaaacc cagattcagt tctattggac
    41041 ctatagctta ctagctcttg tcttgagctg gttggcgagt gtgttaccag cctatctggt
    41101 ggcttggagg gaacttcatg acgaagcagc ccagcttcta cttcctaaac ctcctgtcaa
    41161 aggagctaaa atcttattgg agcgtatcgg ttttatctgg cgtcgtctca gttttactca
    41221 taaggtaaca gcccgcaaca tctttcgtta taagcagaga atgttgatga caatctttgg
    41281 tgtggcaggt tctgtagctc tgctctttgc aggtttggga atccaatctt ctgtagcagg
    41341 agttccgtct aaacagtttc aacaaatcca acagtatcag atgcttgtct ctgaaaatcc
    41401 tagtgcgacc aatcaggaca aggtagagct agcagaagtg ttgaaagggc aggagatact
    41461 agcctaccag aaaatctatt ctaaaacgct agacaaggat ttcaaaggca aggctggtct
    41521 tcaaaacatt actcttatga tgatagagaa ggaagatttg actcccttta tccatcttca
    41581 acatcatcag caggagctga cattaaaaga tggcatcgtt attacagcta aactcgccca
    41641 gctggcaggt gtcaaggttg ggcagacttt agaaattgaa ggtaaggaac taaaggtcgc
    41701 tgctattact gagaactacg ttggtcactt tatttatatg agtcaggcta gctatgagca
    41761 actttacgga cagctacccc aagccaacac ttatctggtc tcattaaggg ataccagtgc
    41821 aactagtatc gaaagtcagg cgggcttgct tatgaatcaa tctgcggtgt ctagcgttgt
    41881 ccaaaatgct tcagccattc gactcttcga ctctgtcgct agctcactca atcagaccat
    41941 gaccatcttg gtcatcgtat cggttctatt agctattgtc atcctttaca atctgaccaa
    42001 tatcaacgta gctgagagaa tccgtgaact ctccactacc aaggttcttg gttttcataa
    42061 taatgaagtc accctctaca tttaccgtga gacgattgtg ctatcccttg tgggaatcgt
    42121 acttggtctg gtagctggtt tctatttaca ccaatttttg attcaaatga tttcgcctgc
    42181 gactattctc ttttatccgc aggtaggctg ggaagtctat gtaatcccag tggcagcagt
    42241 aagcatcatt ttgaccttgc ttggtttctt cgtcaattat tatctgagaa aggttgatat
    42301 gttagaagcc ctgaaatctg tagagtaagg tagttatttt tagctgattg aacttctatt
    42361 tactaatatt caaaaatcct ccgtttcaaa gagcagggaa ctctttgtga cagaggattt
    42421 tttctatagg gctttagcag ctgcaattgc ggcttcgaag tttggctcag aattgatatt
    42481 atccacgtat tcaacgtagc gaatcgtatt gtcagtatcg aggacaaaga ctgcgcgtgc
    42541 taataggtgc cattcgttga tcaagagggc ataatcgcgc ccgaaagaat ggtcaaagta
    42601 gtctgaaagc ataatggcat tgtcaaggcc ttcagcaccg caccaacgtt tttgagcaaa
    42661 aggtaggtcc attgaaacag tcaatacgac cgtgttgtcc agtccagcta attcttcatt
    42721 aaaacgacgt gtttgagttg agcagatgcc tgtatcgata gaaggaacga cactcaagac
    42781 ttttttcttg ccatcaaaat cagccagaga ttttttagaa agatctgttg tagtaagaga
    42841 aaaatcaagc gccttgtcgc cgacttgtag ttgtttacct gtaaagctca caggatttcc
    42901 gagaaaagtt accataggat actccaatct tttttcttcc attgtatctg aaacagtcag
    42961 aattttccaa tgatttgacc ggaaatgtgg gcatagaaaa aacgccagct cacatgagaa
    43021 tgacgttttt cagaggctta ttttgccaat ccttcagcaa tcttgtcaag gttgtatttc
    43081 atcatgctgt agtagctgtc gccttcttta ccttgttctg cgatagagtc agtaaagatt
    43141 tgtgcgtaga ttgggatgtt tgtgtcttga gaaacagttt tcattggacg gtcatccaca
    43201 cttgattcta caaagagtga tggaactttt gtttggcgaa gtttttcaac caaggtcttg
    43261 atttgttcag gagttccttc ttcttcagta ttgatttccc agatgtaggc acttggaaca
    43321 ccataggctt tagagaagta tttgaatgct ccttcgctgg ttacaatgag tttcttttca
    43381 gcagggatct tattaaattt atccttactt tctttatcaa gtttgtctaa cttatcagta
    43441 tattctttga gatttttttc atagaattct ttattgttag ggtctttggc gctcaattgt
    43501 ttagcgatat ttttagcaaa aataataccg ttttcaaggt taagccaagc gtgtgggtct
    43561 tcttttcctt tttcattttg accttcaagg tagataacat caacgccgtc gctgactgcg
    43621 aagtagtctt tgttttcagt tttcttggca ttttctacca attttgtaaa ccaagcattg
    43681 ccacctgttt caaggttgat accgttatag aaaatcaaat cagcctcaga agttttctta
    43741 acgtcttcag gaagtggttc gtattcgtgt gggtcttgcc caatcggaac gatactatga
    43801 aggtcaattt tgtcaccagc aatattttta gtaatatcag cgatgattga gtttgtagca
    43861 acaactttta gtttttgacc agaagttgta tctttttttc cgctagcaca tgctacaaga
    43921 atgattgcag aaagaaagag aacgagtaat gtacctaatt ttttcattag atcctccaat
    43981 ttattagggc tttgcccctt attttaacaa atgtttattt ttcagtttca aatatcgttg
    44041 tttgggagcg ataaagaagc taatgagaaa gaaactagca gctgtaagca cgatactaga
    44101 acctgccgca acattaaaac tatagccaat aaagagtccc aaaactgaag cagtagctcc
    44161 gaaggttgag gaaaggaaaa tcatgctttt caaactatta gcatacagat aagcagttgc
    44221 agctggggta atcagcatgg ctacaatcag gatagttccg acactttgca tggctgtcac
    44281 agacacgaga gtcaggagta ccatgagaag gtagtgatag aaattgacag gcattcccat
    44341 ggctttagcc aagagttcat caaaggaagt tatcaagagt tgcttgaaga aaatccagat
    44401 taacaagagg atggctgccc ccacacccat agtaataaac atatccgtat cttggacggc
    44461 caggatatta ccaaaaagga tatggaaaag gtcagttgaa cttttagcga caccaatcaa
    44521 gatgataccg agggctaaga aagaagaaaa ggtaatgccg atggcggtat cgcttttgat
    44581 aatcgagttt cctttgatgt aggtaatgat gatggcagct agcaatccaa agacaatggc
    44641 tccgataaag aagtcaaggc ccaagatgaa ggagagggct acacctggta agacagcatg
    44701 tgaaatggca tctcccatga gtgacatccc gcgtagaata atgaaacatc ccacagctcc
    44761 agctacaatc ccgacgacaa tagctgttat caaggcattt tgtaggaaat ggaatttttg
    44821 caatccatcg ataaattctg caatcatagg tcacctccat tgaaaaagag ttgattaccg
    44881 taagcttctt ttagattggt ttcggtaaaa gtttcttttg ttggaccaaa ggcaatcact
    44941 tctcgattga caagtaagac ttgatcgaag tagtggggaa tcttgctgag gtcgtggtga
    45001 acgatgagaa ccgtcttccc agcttttttc aaatctctca gcgtattcat gatgatttcc
    45061 tcactgacag agtcaatccc agcaaagggt tcatccaaga ggatatagtc ggcttcctgc
    45121 actaaacatc tggcaatcaa aacccgctgg aattgacctc cagacagttg actaatttga
    45181 cgttcagcgt agtcagctag gccgacgatt tcaagggcct cttgcacttt cttccaatgt
    45241 ttagccttta aacttcgaaa gagaggaata gagggaaata gtcctaacga gacgcattcc
    45301 ttgaccttga tgggaaagtt gtagtcgata ttgatttttt gttcgacata ggcaattcgg
    45361 tgtaaggatt ttttaacttc cttgtcatcg agaaatgcct gaccttgatg tgggataatt
    45421 cccagcatac cttttaatag tgttgatttc ccagcgccgt ttggaccaat gatgccggta
    45481 attgttggtc catggagcac tagtgaaata tccttaagtg ccaacgtttc tttgtaggag
    45541 acactgaggt tttcgatacg tatcataaac ttgtat
//
LOCUS       DAVLVZ010000010        43110 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000010 DAVLVZ010000000
VERSION     DAVLVZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43110
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.010"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(68..265)
                     /locus_tag="VYN77_000515"
     CDS             complement(68..265)
                     /locus_tag="VYN77_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1558021.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(340..1116)
                     /locus_tag="VYN77_000516"
     CDS             complement(340..1116)
                     /locus_tag="VYN77_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV1558022.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            1238..1984
                     /locus_tag="VYN77_000517"
     CDS             1238..1984
                     /locus_tag="VYN77_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1558023.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1997..2977
                     /locus_tag="VYN77_000518"
     CDS             1997..2977
                     /locus_tag="VYN77_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1558024.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3172..3492
                     /locus_tag="VYN77_000519"
     CDS             3172..3492
                     /locus_tag="VYN77_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1558025.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3538..3846
                     /locus_tag="VYN77_000520"
     CDS             3538..3846
                     /locus_tag="VYN77_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1558026.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3863..5161
                     /locus_tag="VYN77_000521"
     CDS             3863..5161
                     /locus_tag="VYN77_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1558027.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            5312..7750
                     /locus_tag="VYN77_000522"
     CDS             5312..7750
                     /locus_tag="VYN77_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1558028.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERAHKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7769..8437
                     /locus_tag="VYN77_000523"
     CDS             7769..8437
                     /locus_tag="VYN77_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1558029.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            8455..9543
                     /locus_tag="VYN77_000524"
     CDS             8455..9543
                     /locus_tag="VYN77_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1558030.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            9998..12499
                     /locus_tag="VYN77_000525"
     CDS             9998..12499
                     /locus_tag="VYN77_000525"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1558031.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTS
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWIGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGDVKEAAYTEDGLHVNSDFLDELNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            12560..13621
                     /locus_tag="VYN77_000526"
     CDS             12560..13621
                     /locus_tag="VYN77_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558032.1"
                     /translation="MRTQSHKEIVEEYSASRGSYEDCLNYVENTVKNIIKSQSINVHE
                     IIGRVKTVESLEGKVKRKNYSNLAEITDLCGIRIITYFSDDVDKIAELISQEFEVDVE
                     NTIDKRKSEDPTKFGYVSLHYVVGLKEENTSPTLYGRFKNIKLEIQIRTVMQHAWAEI
                     EHDLGYKSKEDIPDQYRRQFARLAGLIELADDDFVQLKNNINNYEQEIKKKLPTSKKE
                     LPIDSSTLMTYVKEDQNYIELLNTIKSSDMDIDFNIDSSFLSTMIQRAKKLDLRTIGD
                     IAELFEKYKDIFLKVLRIEEDIEPCMSISALTPLLHITNVLESVNNINISDEEFMKVS
                     DDEFSDVFGISYPIDVYEG"
     gene            13848..14543
                     /locus_tag="VYN77_000527"
     CDS             13848..14543
                     /locus_tag="VYN77_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1558033.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMEELVSLLNQMLARKDRFYYAIIDRSTGKVLG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAF
                     NLPSRRAAERLGFIYEGTFRQAVVYKGRTRDTDWLSMTDKDWPKVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            14555..14971
                     /locus_tag="VYN77_000528"
     CDS             14555..14971
                     /locus_tag="VYN77_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1558034.1"
                     /translation="MITIKKQEFVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLAI
                     YVALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEQTERTLGFYCSMGFEILSTYDCIGMTWINREK"
     gene            complement(15823..15915)
                     /locus_tag="VYN77_000529"
     CDS             complement(15823..15915)
                     /locus_tag="VYN77_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1558035.1"
                     /translation="MMELILKTIIGPIVVGVILRLVDKWLNKDR"
     gene            complement(16256..16348)
                     /locus_tag="VYN77_000530"
     CDS             complement(16256..16348)
                     /locus_tag="VYN77_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1558036.1"
                     /translation="MMELVLKTIIEPIVVGVVLRLVDKWLNKDK"
     gene            16508..17479
                     /gene="ruvB"
                     /locus_tag="VYN77_000531"
     CDS             16508..17479
                     /gene="ruvB"
                     /locus_tag="VYN77_000531"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1558037.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNDLEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGDGVINDVITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRMATAKAYEHLGYEYNEK"
     gene            17460..18033
                     /locus_tag="VYN77_000532"
                     /pseudo
     CDS             17460..18033
                     /locus_tag="VYN77_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
     gene            18264..19022
                     /locus_tag="VYN77_000533"
     CDS             18264..19022
                     /locus_tag="VYN77_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1558038.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            19031..19834
                     /locus_tag="VYN77_000534"
     CDS             19031..19834
                     /locus_tag="VYN77_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1558039.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFVLTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            19856..21115
                     /gene="rseP"
                     /locus_tag="VYN77_000535"
     CDS             19856..21115
                     /gene="rseP"
                     /locus_tag="VYN77_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1558040.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIESILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            21128..22981
                     /locus_tag="VYN77_000536"
     CDS             21128..22981
                     /locus_tag="VYN77_000536"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1558041.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTSEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            23080..24459
                     /gene="bglA"
                     /locus_tag="VYN77_000537"
     CDS             23080..24459
                     /gene="bglA"
                     /locus_tag="VYN77_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1558042.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            24651..26459
                     /gene="glmS"
                     /locus_tag="VYN77_000538"
     CDS             24651..26459
                     /gene="glmS"
                     /locus_tag="VYN77_000538"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1558043.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLSLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHSYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            26578..27627
                     /locus_tag="VYN77_000539"
     CDS             26578..27627
                     /locus_tag="VYN77_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1558044.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(27727..31524)
                     /gene="spuA"
                     /locus_tag="VYN77_000540"
     CDS             complement(27727..31524)
                     /gene="spuA"
                     /locus_tag="VYN77_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1558045.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYRTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            31941..32354
                     /gene="rpsL"
                     /locus_tag="VYN77_000541"
     CDS             31941..32354
                     /gene="rpsL"
                     /locus_tag="VYN77_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1558046.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            32374..32844
                     /gene="rpsG"
                     /locus_tag="VYN77_000542"
     CDS             32374..32844
                     /gene="rpsG"
                     /locus_tag="VYN77_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1558047.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            33269..35350
                     /gene="fusA"
                     /locus_tag="VYN77_000543"
     CDS             33269..35350
                     /gene="fusA"
                     /locus_tag="VYN77_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1558048.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            35454..39845
                     /locus_tag="VYN77_000544"
     CDS             35454..39845
                     /locus_tag="VYN77_000544"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1558049.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVSRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            39947..40210
                     /locus_tag="VYN77_000545"
     CDS             39947..40210
                     /locus_tag="VYN77_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1558050.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            40203..40481
                     /locus_tag="VYN77_000546"
     CDS             40203..40481
                     /locus_tag="VYN77_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV1558051.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            40502..40654
                     /locus_tag="VYN77_000547"
     CDS             40502..40654
                     /locus_tag="VYN77_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558052.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            40676..41917
                     /locus_tag="VYN77_000548"
     CDS             40676..41917
                     /locus_tag="VYN77_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1558053.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEAAPVKAWEEHAAILKSKANMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            41927..42157
                     /locus_tag="VYN77_000549"
     CDS             41927..42157
                     /locus_tag="VYN77_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1558054.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            42380..43102
                     /locus_tag="VYN77_000550"
     CDS             42380..43102
                     /locus_tag="VYN77_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1558055.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT        12814 a         7599 c         9765 g        12932 t
ORIGIN      
        1 agagatgttt acatcttatt tcaattcgct atattagagt aaaattctct acaaaaagaa
       61 gaatagccta ttttactatt cttccgagtg atttcaattt ctttggggaa atatggagat
      121 actttttaaa tactttggca aaatatttat agtctgaaaa tccaacttgg tcagagatag
      181 cgcttaaaga ggagttggga gattcagcca tcttatctat agcttgtttc aggcgatatt
      241 gtaagatata ttcatttaga gtcatgggga agtcttcttt gataacctta tatagatgct
      301 tgttatctgc aaatagctag agcatttcaa ggaaataaac tagaaataac aattaatatg
      361 gtggttcaga aatacctttt gataatcaat aagatcttca ggatgaagtg cgttgattcc
      421 atccatttca tatttccgat ttaataaacg atatttgttt tcaccaaatt gatgaaaagg
      481 gagtagttga acttggtcga tatttaatga gttaaataga gtagcgaatt tttctgcatc
      541 ctctaaacta ttgttaaaat taggaataac tgggattctt aaaacgatag ttttattttg
      601 agaaaaagca taatgaatgt ttttaataat catttgatta aaaaccccag tcactttttt
      661 atgttttata gaattataat gttttaggtc tgtgtagata aaatccacat attgaattaa
      721 atcgataaat ttttcatgat caacaaaggc agtagtttca atggcagtgt gtatgtgatg
      781 ttctttagct gattttaaga tggctttagc aaattcaaac tgagcaaata tttcacctcc
      841 tgataaagtt aaacctccac cggattcttc gtaaaattct ttgtctttta atacctctgt
      901 aataatttct tctacagtct tttcttcacc gactaaggtg aatttctctc gttgagcatc
      961 tttcattttt tcaggtttca ttctttgaga ttcaggatta gaacaccatg gacagcgcag
     1021 aggacatcct tttaaaaaaa cagttgtacg aatacccggg ccgtcatgaa ttgaaaagtg
     1081 ttgaatatta aaaataattc ctttagatat ttccataaga gttcctcctc ttcataagtc
     1141 tatcctatca caaaaacgaa agaaaaacaa ttacgaacga aaggttaatt tgttgttttt
     1201 tgctttttat tttggtagaa taaaacggag gtgtaaaatg aaaagattgg aacaaattat
     1261 taaattagta tcagaatatg aaaagatcga cgttaataca ttatcggaaa aattaaatgt
     1321 atcgaaagta acgattagaa aagatttaga taaattagag tcaaaaggtt tattacacag
     1381 agagcatgga tatgctgtat taaatagtgg agatgactta aatgtacgtt tgtcaattaa
     1441 ttatgaaatt aagagaaaaa ttgttcagga agcagtaaaa ttggtgtcag ataatgaaac
     1501 aataatgata gaatctggat cgacctgtgc tttacttgct gaggaaattt gcaagcaaaa
     1561 aagaaatgtt acgattgtaa caaattcatt ttttatagca aattttgtga gagcttatga
     1621 ttcatgtcgt attattgttc ttggtggaga gtttcagaag gattcacagg ttactgtggg
     1681 acctttatta aaagaaatga tacagacttt tcatgtgcat caagcttttg ttgggacaga
     1741 tggttacgat aaagagatgg gctttacagg aaaagattta atgcgcagtg aggtagttca
     1801 atatatttca gcagcgtcgg ataaagtcat tgtactaact gactcaagta aatttgataa
     1861 aaggggtaca gtaagaagat ttgctttaag tcaagtctat gaagtaataa cagacgaaaa
     1921 actttctaaa caaaatatag ctacattaga aaatgctggg ataatggtta aggtagtttc
     1981 gtaagaggtt aagtgtatga atcaagatag gaataaactg ctctctaaaa ttgcttatct
     2041 gtattatatt gaaaacttaa atcagtcaca aatagcagca aaattaggaa tttatagaac
     2101 ctctattagt agaatgttaa cagaagcaag gaatgcagga attgttaaaa ttgaaataga
     2161 gaattttgat accaatatgt ttaagttgga aaattatgta aaagaaaaat atggtctgga
     2221 aagtttagaa attattccaa atgaatttga tgataatcca acaattttat ctgaaagaat
     2281 ttctcaagtt gcagcaggtg tccttaggaa tctaattgat gataatatga aaattggctt
     2341 ttcttggggg aaaagtttaa gtaatttagt agatttaatt cacagtaaaa gtgtccgaaa
     2401 tgttcacttc tatcctctag caggtggtcc tagtcacata cacgctaaat accatgtgaa
     2461 tacactgatt tatgaaatgt ctagaaaatt tcatggagag tgtacattta tgaatgcaac
     2521 gattgtgcaa gaaaataaat tgttagcaga tggtattttg caatcaagat attttgaaaa
     2581 tttgaaaaat agttggaaag atttagatat agctgtagtc ggaattggtg attttagcaa
     2641 taaaggaaag catcaatggt tagacatgct tacagaggat gactttaaag aattgaccaa
     2701 agttaaaact gtaggagaaa tttgttgtcg attttttgat tcaaaaggta aagaagtcta
     2761 tgaaaactta caggaaagaa cgatagcaat ctctttggaa gatttaaaaa atattcctca
     2821 aagtttagct gttgcttacg gtgatacgaa agtatcttcg attctttctg tcttgcgtgc
     2881 taatttagta aatcatttga ttacagacaa aaatacaatt ttaaaagttt tggaagaaga
     2941 tggggatttg acttttagag agattctagg tgagtgaaaa tgatagactg attcagttta
     3001 tcgtttttct ttttagttga ttgcacattt gtgcttatat aaacaaaaat agtttatctg
     3061 ttgtttttgg attgacaact ttattatgta gttgtattct atagttacaa aagaaaattt
     3121 taaaatttca aatgaaaaaa gctttttaca tagtgaaatg aggaggaatt tatggaaatt
     3181 attgttccag atcaaattat catgggttta attttatatg ctggcgatgc gaaacaacat
     3241 atttataaag cattagatta cataaagaac ggtacatgtg aacgatgtga agaagaaatc
     3301 cagttagctg atgcagcctt attagaagct cataatctac aaacaaaatt cttggcacag
     3361 gaagcgtctg gtacaaagac agaaattaca gctctctttg ttcattcaca agatcatctc
     3421 atgactagta tgacggagat taatttaatc aaagaaatta ttagtttgag aaaagaactt
     3481 cataaaaaat aatactagag tattatcatt gttattaaca tagaggagga aaacataatg
     3541 gtgaagattg gtttgttttg tgcagcaggt ttttctactg gtatgcttgt aaataatatg
     3601 aaaattgcag cgcaatctag tggagttgag gcagaaatag aggcgttttc tcagtctaaa
     3661 ttagcggatt atgcgccaaa tatagatgtt gcactattgg gtccacaagt tgcttataca
     3721 ttagataaat caaaagaaat ttgtgataag tgtgatgttc cgatagctgt tattccgatg
     3781 atggactatg gtatgttaga tgggaaaaaa gtattagatt tggccctatc tttgattagt
     3841 gggtaagaaa aggagattta ttatgtcaaa gatggatgtt cagaaaatca ttgcaccgat
     3901 gatgaagttt gtgaatatgc gtggcattat agctctaaaa gatgggatgt tagcaatttt
     3961 gccattgaca gtagttggta gtttgttctt gattatggga caattgccgt tcgaaggatt
     4021 aaataagagc attgctagtg tttttggagc taattggaca gagccgttta tgcaagtata
     4081 ttcaggaact tttgctatta tgggtctaat ttcttgtttt tcaattgcct attcttatgc
     4141 taagaatagc ggagtagagg ctttaccagc tggagttcta tctgtatctg cattctttat
     4201 tttgctaaga tcatcttata tccctaaaca aggtgaggcg attggggacg ctattagtaa
     4261 agtttggttt ggaggccaag gaattatcgg tgctatcatt ataggtttgg tagtaggaag
     4321 tatttatacc ttctttataa agagaaaaat tgttattaag atgccagaac aagttccaca
     4381 agctattgcg aaacagtttg aagcaatgat tccagcattt gtaattttct tatcttctat
     4441 gattgtatat attttagcga agtcattgac taatggcgga acattcatag aaatgattta
     4501 ttctgctatt caagttccgt tgcaaggttt aactggatct ttgtatggtg ctattggaat
     4561 tgcattcttt atatcatttt tgtggtggtt tggtgttcat gggcaatcgg tagtaaatgg
     4621 agtagtgaca gctctgcttt tatctaatct tgatgctaat aaagctatgt tagcctctgg
     4681 taatctatca ttagaaaatg gcgcacatat tgttactcaa caatttttag attcattttt
     4741 aattctatca ggttcaggga ttacgtttgg tcttgtagtt gccatgcttt ttgcagcaaa
     4801 atcaaaacaa taccaagcct taggaaaagt tgcagctttt ccagcaatat ttaacgtaaa
     4861 tgagccagtt gtatttggat ttccgattgt catgaatcca gttatgtttg tacctttcat
     4921 tcttgttcct gtacttgcag ctgtgatagt atatggagct attgcaacag gtttcatgca
     4981 gccattctca ggggtaacat tgccttggag tacaccagct attctatcag gatttttggt
     5041 gggtggatgg caaggtgttg tgactcagtt attagtattg gtgatatcta cagtggttta
     5101 ctttccattc tttaagatac aagatcgttt agcttaccaa aatgaaatca aacaatctta
     5161 gaggtatttg tgtgttactg ttaaactcac acatttgtgc taaaaattag agagttaaaa
     5221 tttttctagt taaaagcttg aaaatttcta taaaaatcgg tattatattt tcgaaagaaa
     5281 taaaaatatt ttcgaaagaa aggtggttac gatggtaaat acagaagtag caagaacaac
     5341 aatcaagaca gaatattttg gcagccttac tgaaaggatg aacaaatatc gagaagatgt
     5401 tttaaataaa aaaccttata ttgatgctga gagagcagtt ctagcaacac gcgcctatga
     5461 acgatacaag gaacaaccta atgtcctaaa acgtgcatat atgctgaaag aaattttgga
     5521 aaatatgtct atctatattg aagaagaatc tatgattgcg ggaaatcaag cttcttccaa
     5581 taaagatgct cctatttttc cggaatatac gctagaattt gttctcaatg agttggatct
     5641 ttttgaaaag cgtgatggag atgttttcta tattacagaa gaaacaaaag aacaacttag
     5701 aagtattgct ccgttttggg aaaataataa tttacgtgct agagctggtg ccttattacc
     5761 tgaagaagtg tctgtttata tggaaacagg attcttcggt atggaaggta agatgaattc
     5821 tggagatgct cacttagcag ttaactatca gaaacttttg caatttggtt taagaggttt
     5881 tgaagagcgg gctcataaag caaaagtagc tctagattta acagatccag caagtattga
     5941 taaatatcat ttttacgact ctatatttat cgtaatcgat gctattaaag tatatgcaaa
     6001 gcgctttgtt gctcttgcta aaagtttagc cgaaaatgca aatcctaaac gtaagaaaga
     6061 attacttgag attgcagata tttgctctag agtcccatat gaaccggcaa ctacttttgc
     6121 agaagctatt caatcagttt ggtttattca atgtatttta caaattgaat ctaatggcca
     6181 ctctctttca tatggccgtt ttgatcaata tatgtatcca tatatgaagg ctgatttaga
     6241 aagtggtaaa gaaacagaag atagcattgt tgaacgtctg acaaatcttt ggattaagac
     6301 aattacaatt aataaggttc gcagtcaatc acatacattt tcttcagcag gaagtccttt
     6361 atatcaaaat gttacaattg gtggacagac tcgagataag aaggatgctg ttaacccatt
     6421 atcttatttg gtattaaaat cagttgcaca aacccatcta ccgcaaccta atctaactgt
     6481 acgttaccat gcaggtttag atgctcgttt catgaatgag tgtattgaag tgatgaaact
     6541 tggttttggt atgcctgcat ttaataatga tgagattatt attccttctt ttattgcaaa
     6601 aggagtattg gaagatgatg cttatgatta cagtgccatt ggatgtgttg aaacggcagt
     6661 tccagggaaa tggggctatc gttgcacagg tatgagttat atgaacttcc ctaaggttct
     6721 acttatcacg atgaatgatg gaattgatcc ggcttcgggt aaacggtttg caccaagctt
     6781 tggtcatttt aaggatatga agaacttttc tgaattagaa aatgcttggg ataaaacact
     6841 aagatatttg acacgaatga gtgttattgt tgaaaattct attgatttat cattggaacg
     6901 agaagttcct gatattctat gttcagcatt gactgatgat tgtattggtc gtggaaaaca
     6961 ccttaaagaa ggtggagcag tatatgatta tatatcagga ttgcaagttg gaattgcaaa
     7021 tttgtcggat tcgttagctg caattaaaaa attggtgttt gaggaagaac gtataagccc
     7081 aagtcaactt tggcatgcac tggaaacaga ttatgccgga gaagaaggta aggtcattca
     7141 agaaatgttg attcatgatg cacctaagta tggtaatgat gatgattatg ctgacaaatt
     7201 ggttactgct gcttatgaca tttatgttga tgaaattgct aaatatccta atacacgtta
     7261 tggaagaggg cctattggag gaattcgtta ttcaggaaca tcttctatat cagccaacgt
     7321 agggcaggga cgtggaacat tagcaactcc agatggacgc aacgcgggta caccgttagc
     7381 agagggttgt tcaccatcac ataatatgga tcaacacggc cctacatctg ttttaaaatc
     7441 tgtttcaaaa ttaccaacag atgaaatcgt aggtggggtt ctcttaaatc agaaagtaaa
     7501 tcctcaaacg ttagccaaag aagaagataa attaaaacta attgctttgt tacgaacatt
     7561 ctttaatcgt ttacatgggt accatattca atacaatgtt gtttccagag agacgctgat
     7621 tgacgctcag aaacatcctg aaaaacacag agacttaatt gttcgtgttg caggatactc
     7681 tgcattcttc aatgttcttt ctaaggcaac ccaagatgac attataggac gtactgagca
     7741 tactttgtaa aataaagagg ttctttttat ggaatttatg cttgacacat taaatttaga
     7801 tgagattaaa aagtggtctg aaattttgcc gctagctggg gtaacttcaa atcccactat
     7861 tgcaaaaaga gagggttcta ttaatttttt tgaacgaatc aaagatgtaa gagaattgat
     7921 tggctctaca ccctctattc atgttcaggt gatttctcaa gattttgaag gcatcttaaa
     7981 ggatgctcat gaaattcgaa gacaagcagg agatgatata tttatcaaag tacctgttac
     8041 tccagctgga ttacgtgcaa taaaggtgct aaaaaaagag ggctaccata tcactgcaac
     8101 agctatttat acagttattc agggattatt agctatcgaa gcaggagcgg attacctagc
     8161 tccatattat aatagaatgg aaaatctgaa cattgattca aattctgtca ttcgtcaatt
     8221 agctcttgct attgatagac agaactctcc tagtaagatt ttagctgcat cctttaaaaa
     8281 tgtagcacaa gtaaataatg ctttagctgc aggtgcgcat gctgttacag caggagcaga
     8341 tgtttttgaa tcagctttcg ccatgccatc tatccaaaag gcggttgatg atttttctga
     8401 cgattggttt gttactcaaa atagtcgttc catttagata gagaggaaat atatatgaga
     8461 atttttgcta gtccttctag atatattcag ggggaaaatg ccttgtttga aaatgccaaa
     8521 tcaattttgg atttgggaaa ttaccctatt ctattatgcg atcagttggt ttatgatatt
     8581 gttggaaaac gatttgaaga ttacctacat aggtatggtt tccatattgt tctggcgcta
     8641 tttaatggtg aagcttctga caatgaaatc aatcgagttg ttgccttggc tgagaaagaa
     8701 aattgtgata gtattatcgg tcttggtggg ggaaagacga ttgatagcgc aaaagctatt
     8761 gcagatttga ttgaaaagcc tgttattatt gctccaacaa ttgcatcgac cgacgcacct
     8821 gtatctgctt tatctgttat ttatacagat gaaggtgcat ttgatcatta tctattttat
     8881 tctaaaaatc cagatttagt tttggttgat acaaaagtta tttcacaagc ccctaagcgt
     8941 ttattagcgt ctggtattgc agatggttta gcaacttggg ttgaggcgcg tgcggttatg
     9001 caggcaaatg gaaaaactat gttgggacaa cagcaaacat tggctggagt tgcaattgcg
     9061 aagaaatgtg aagaaacgct gtttgcagat ggtttacagg ctatggcagc ttgtgaagct
     9121 aaagtggtga caccagcatt agaaaatatt gttgaagcta atactttatt gagtggtcta
     9181 ggttttgaaa gtggaggatt agctgcggcg catgcaattc ataatggttt tactgcattg
     9241 acaggtgaca ttcatcattt aacacatggt gaaaaagtag cttatggaac tttagtacaa
     9301 ctattattgg aaaatagacc taaagaagaa cttgataagt atattgagtt ttacaaaaaa
     9361 attggtatgc caacaactct aaaagaaatg catttggatc aagttggata tgatgattta
     9421 ataaaagttg gtaaacaagc aactatggag ggtgagacaa ttcatcagat gccgtttaag
     9481 atttcgcctt cagatgttgc tcaagctatt atcgctgtag atgcctatgt aaattcaaaa
     9541 taaacaataa ggactactgt tttccaaatg gtagtctttt attgatcctc gtattgaatt
     9601 ctatagcaga ttgaaataag atgagaacaa atcgattggg aaagtaaaat taatttctat
     9661 aaatgtttta gcaattgttt cgtactattt cagattcagt ccactatacg ttcttcataa
     9721 atcaaaaagc gacataggta gtcggctatt tattgtgaat acattaatta gcattccagt
     9781 tttatcttcg gtctaaaata agtattttgt gctatacgag ataagcgtct tgacttactc
     9841 cttgatttac tgcataacaa tgggataaaa agtgggagat agagcaattc atagtcatca
     9901 aaattaatga gatacagtat acagtttttt ctttaaacac atttcaaatt ccctcaaaaa
     9961 tggtataata gtaacatcac aaaattggag agagaccatg agtttttaca atcataaaga
    10021 aattgaacct aagtggcagg gctactgggc agaacatcat acatttaaga caggaacaga
    10081 tgcatcaaaa cctaagtttt atgcgcttga tatgttccct tatccgtctg gagctggtct
    10141 gcatgtagga cacccagaag gttatactgc aaccgatatc ctcagtcgtt acaaacgtgc
    10201 gcaaggctac aatgtccttc acccaatggg ttgggatgct tttggtttac ctgcagagca
    10261 atacgctatg gatactggta atgacccagc agaatttaca tcggaaaaca ttgccaactt
    10321 caaacgtcaa atcaatgcgc ttggattttc ttatgactgg gatcgtgaag tcaacacaac
    10381 agatccaaac tactacaagt ggactcaatg gattttcacc aagctttacg aaaaaggctt
    10441 ggcctatgaa gctgaagtgc cagtaaactg ggttgaggaa ttgggaactg ccattgccaa
    10501 tgaagaagtg cttcctgacg gaacttctga gcgtggaggc tatccagttg tccgcaaacc
    10561 aatgcgccaa tggatgctca aaatcacggc ttacgcagag cgcttgctca atgacttaga
    10621 tgaactagat tggtcagagt ctatcaagga tatgcaacgc aactggattg gtaaatcaac
    10681 tggtgccaat gtaactttca aagtaaaagg aacagacaag gaatttacag tctttactac
    10741 tcgtccggac acacttttcg gtgcgacttt cactgtcttg gctcctgaac atgaattagt
    10801 agacgctatc acaagttcag agcaagcaga agctgtagca gactataaac accaagccag
    10861 ccttaagtct gacttggctc gtacagacct tgctaaagaa aaaactggtg tttggatagg
    10921 tgcctatgcc atcaaccctg tcaatggtaa ggaaattcca atctggattg cggattacgt
    10981 tcttgctagc tatggtacag gtgccgttat ggctgtgcct gcccacgacc aacgtgactg
    11041 ggaatttgcc aaacaatttg accttccaat cgtcgaagta cttgaaggtg gagatgtcaa
    11101 agaagctgcc tacacagagg atggcctgca tgtcaattca gacttcctag atgaactcaa
    11161 caaagaagac gctattgcca agattgtggc ttggttggaa gaaaaaggct gtggtcagga
    11221 gaaggttacc taccgtctcc gcgactggct ctttagccgt caacgttact ggggtgagcc
    11281 aattccaatc attcattggg aagatggaac ttcaacagct gttcctgaaa ctgaattgcc
    11341 gcttgtcttg cctgtaacca aggatatccg tccttcaggt actggtgaaa gtccactagc
    11401 taacttgaca gattggcttg aagtgactcg tgaagatggt gtcaaaggtc gtcgtgaaac
    11461 caacactatg ccacaatggg ctggttcaag ttggtactac ctccgctata ttgatccaca
    11521 caatactgag aaattggctg atgaggacct cctcaaacaa tggttgccag tagatatcta
    11581 cgtgggtggt gcggaacatg ctgtacttca cttgctttac gctcgtttct ggcacaaatt
    11641 cctctatgac ctcggtgttg ttccgactaa ggaaccattc caaaaactct ttaaccaagg
    11701 gatgattttg ggaacaagct accgtgacca ccgtggtgct cttgtggcaa ccgacaaggt
    11761 tgaaaaacgt gatggttcct tcttccatgt agaaacaggg gaagagttgg agcaagcgcc
    11821 agccaagatg tctaaatcgc tcaagaacgt tgttaaccca gacgatgtgg tggaacaata
    11881 cggtgccgat acccttcgtg tttatgaaat gtttatggga ccactcgatg cttcgattgc
    11941 ttggtcagaa gaaggtttgg aaggaagccg taagttcctt gaccgagttt accgtttgat
    12001 tacaagtaaa gaaatccttg cggaaaacaa tggtgctctt gacaaggctt acaacgaaac
    12061 agtcaaagct gttactgagc aaattgagtc tctcaaattc aacacagcta ttgcccaact
    12121 tatggtcttt gtcaatgctg ctaacaagga agataagctt tatgttgact atgccaaagg
    12181 ctttattcaa ttgattgcac catttgcacc tcacttggca gaagaactct ggcaaacagt
    12241 cgcagaaaca ggtgagtcaa tctcttatgt agcttggcca acttgggacg aaagtaaact
    12301 ggttgaagac gaaatcgaaa tcgttgtcca aatcaaaggt aaagtccgtg ccaaactcat
    12361 ggtcgctaaa gacctatcac gtgaagaatt gcaagaaatt gctctagcgg atgaaaaagt
    12421 caaagcagaa attgacggta aggaaatcgt gaaagtgatt agtgtaccaa ataaactcgt
    12481 taatatcgtt gtgaaataat tgtaaattat agttaattaa aaagagagac agtttttgtt
    12541 cactttttgg gaggtattaa tgaggactca aagtcataag gagattgttg aagaatattc
    12601 tgcttcacgg gggagttatg aagattgctt aaattatgta gagaatactg taaagaatat
    12661 tattaaaagt caatctatta acgttcatga aataattggg agagtaaaaa ctgtagagtc
    12721 tcttgaaggc aaagtaaaaa ggaaaaatta ttctaattta gctgagatta ccgatttatg
    12781 cggtattcgt atcatcacgt actttagtga tgatgttgat aaaatagctg agttaatcag
    12841 tcaagaattc gaggttgatg tagagaatac tattgataaa cgtaaaagtg aggatccaac
    12901 aaaatttggt tatgtttcac ttcattatgt ggtgggttta aaagaagaga acacttcacc
    12961 tactttatat ggtagattta agaatataaa attagagata caaattagga cagttatgca
    13021 acatgcctgg gctgaaattg aacatgattt gggatataaa agtaaggaag atatacctga
    13081 tcaatacagg agacaatttg ccagattggc gggactaatt gaattggctg atgatgattt
    13141 tgttcagttg aaaaataata ttaataacta tgagcaggag ataaagaaga aattacctac
    13201 ttcgaaaaaa gaattaccga tagatagcag cacgcttatg acttatgtaa aagaagatca
    13261 aaattacatt gaactattga atacgattaa atcatcagat atggatatag attttaatat
    13321 tgatagttct tttctatcta ctatgattca gagggcgaaa aaattggatt tgaggactat
    13381 aggagatata gctgaattat ttgaaaaata taaggatatc tttctaaagg tattgagaat
    13441 tgaggaggat attgaacctt gtatgagtat atcggcattg actccattat tgcatatcac
    13501 taatgtgtta gaatcagtta ataatattaa tatttctgat gaagaattta tgaaagtttc
    13561 tgatgatgaa ttttcagatg tatttggtat atcgtaccca attgatgttt atgaaggtta
    13621 aaaagtaatt ttttaaatat gaaaagaggg gtttcctatt tgactaagtt gaataattcg
    13681 tagtgttgct ctttctaaag tgaaggtaac taaactcgtc aatatcgttg ttagataaga
    13741 aataaatcct tcagagtaga atctggagga ttttttgaat cttcttatga aagtatgata
    13801 tactatgggc aactataaag tttgaaaagt gaaacaagga gaataagatg ccagtaaatg
    13861 aatatggtca gatgattggg gagtcaatgg aaggttatac accaggtgaa ctgccttcta
    13921 ttgatttctt agaaggacgt tatgctcgga tagaggctct ctcggtagaa aaacatgcgg
    13981 aggatttgtt agctgtttat ggcccggata ctcctcggga gatgtggacc tacctctttc
    14041 aggaaccagt agcagacatg gaggaactgg tcagcctttt aaatcagatg ttggctcgta
    14101 aggaccgttt ttactatgca atcatagaca ggtcaactgg taaggttttg ggaacttttt
    14161 ctctcatgcg cattgaccag aataaccgag taatagaagt gggagctgtc actttttctc
    14221 cagaactcag ggggacacgg atagggacag aagcccagta tctactggct cgctatgtct
    14281 ttgaagagct taactatcgt cgctatgagt ggaaatgcga tgcttttaat ctgccatcaa
    14341 gacgagctgc agaacgtttg ggctttattt atgaaggaac cttccgtcag gcagtggttt
    14401 ataaggggcg tacgagggat acggattggt tgtctatgac tgataaggac tggcccaaag
    14461 tcaaagatcg tttggaaaca tggttgcgtc ctgaaaattt tgataaaaat ggacagcagt
    14521 acaagagctt gagagagctt taagaggtgt tgagatgatt actattaaaa agcaagaatt
    14581 tgtcaagcta gaggatgttt tgcatctcta tcaggctgtc ggttggacaa attataccca
    14641 tcaaccagag atgctggagc aggccttatc tcattcatta gcgatttatg tggcgcttga
    14701 tggcgatgct gtggtgggct tgattcgttt ggttggagat ggattctcat cagtattggt
    14761 tcaggattta atcgttttac caatctatca acgtcaaggg attggtagtg ccctaatgaa
    14821 agaggcttta gaggattaca aagatgccta tcaagtccag ctggtaacag aacagacaga
    14881 aagaaccttg ggcttctatt gttctatggg ctttgaaatc ttatccacct atgattgtat
    14941 aggaatgact tggataaaca gagaaaaata ataaaacttg gttgttctta agcaaagttt
    15001 aaggctggtc tagtatcata tagtcattaa ataaagacct cctaacttta tttaatgaaa
    15061 tcctaaaact ttttttcatc ataatctcct aatgaagtca cctaatcagg tggctttttt
    15121 gtagggtgag gtgatgctga tagaaatttt tttaaaaatg gtataatttg agaaaagtta
    15181 agctagtttt aagcttcgtc ttgtatcata tagttattaa ataaagacct cctaacttta
    15241 tttaataaaa tcctaaactt ttctttttca taataatctc ccttaactcc acccaatcag
    15301 gtggagtttt ttggctctat ttcaggcttt tggggactat tctaaaaata atttttcgat
    15361 atttttcggt atttttcgga ttttggtcgg ggaattggcg gggacttttt gagattttgg
    15421 cggggatttt tttagcgaat atgactaaga aataggtcgg ttgtcgcttc agcaagttcg
    15481 tcctcaactt gattgtaagt tgctgttaat tgaaggactg gaactgctgt aataatgatt
    15541 ttgcttgtcc agtagtggcg tattaaatga ggagtgacat ataagattgt ctcttcgttt
    15601 actaaatcaa aatttctatg aaattgatag aatccctaac tttctcttaa aattgaaagt
    15661 cttgttgtaa agcggttaaa gcttgagaag ctgaggatgt aaagttttga caaattttgt
    15721 gaactgtggg ataatggaga ggaattaagg attagttcta tatcatggac tagaaaagac
    15781 cccaagctaa cttccacatt aagcttgggg tcttttttga cactatctat ctttgtttag
    15841 ccatttatcg actaagcgaa ggatgacacc gaccacaatc ggtccgatga tagttttaag
    15901 gattagttcc atcatgggct atctcacctc ctttcgcaag cggtgtagca gtgccgtaga
    15961 atattatacc acataagtgc taaactcggg agtcacccaa ccaggtggag ttttttgctc
    16021 tatttcaggc ttttggggac tattttaaac atcattttcc gataactttc acttaatctt
    16081 ataaacttta ttggacagat tctaaagttg gagaagctga atggatgaaa atataaaact
    16141 ttgacaaaat gtgtgaacta tgggataata aaaaggaatt gagtactagt tctatatcat
    16201 aggctagaaa agaccccaag ctcacgacca aataagcttg aggtcttttt tgacactatt
    16261 tgtccttgtt tagccattta tcgactaatc gaagaacgac accgaccaca attggttcga
    16321 tgatagtttt gagtactaat tccatcatgg gctatctcac ctcctttcgt aggcggtgta
    16381 gaagtgccgt ataatattat accacataag tgctaaactc gggaagcacc aatccagtga
    16441 cttttttgtt tgtggcttgg ttttttgata taatagagcc atgagtagaa ttttagataa
    16501 tgagataatg ggggacgagg agttagtaga acgcacgctc cgtcctcagt acttacgtga
    16561 atatattggg caggataagg ttaaggacca gcttcaaatc tttatagaag ctgccaaaat
    16621 gcgagatgaa gcgctggatc atgtcctctt atttgggcct ccaggtttgg ggaaaacgac
    16681 catggctttt gttattgcca acgaactagg tgtcaatctc aagcagactt cgggtccagt
    16741 cattgaaaaa gccggagatc tggtagcaat tttgaatgac ttagagcctg gggatgtcct
    16801 ctttattgat gagattcatc gcttgcctat gtcggtggaa gaggtgcttt atagtgccat
    16861 ggaggacttt tacatagata ttatgattgg ggctggtgaa ggcagtcgca gtgttcattt
    16921 ggagttacca ccttttacct tgattggtgc gacgactcgg gctggtatgc tctcaaatcc
    16981 actacgggca cgttttggga ttacaggtca tatggagtat tatgctcatg ctgacttgac
    17041 ggaaattgtc gagcggacgg cagatatttt tgagatggaa atcactcatg aggcagcgtc
    17101 tgagttggcc ctacgtagcc gtggaacccc tcgtattgcc aatcgtctcc tcaagcgcgt
    17161 gcgcgatttt gcccagatta tgggggatgg ggttatcaat gatgttatta cggataaggc
    17221 tttgaccatg ctggatgttg accatgaagg tctggactat gtggaccaaa aaatccttcg
    17281 gaccatgatt gagatgtaca gtggagggcc tgttggttta ggaactcttt ctgtgaatat
    17341 tgccgaggag cgcgagacgg tggaggacat gtatgaacct tacttaatcc aaaaaggttt
    17401 tatcatgcgg acacggtctg gacggatggc gactgctaag gcatatgagc atttggggta
    17461 tgagtacaat gaaaaataag caagaaattc tagaggcttt tagaaaaaat ccagatatga
    17521 tggctattct gactatcatt caaaacctgg agttgacaga ttcctggttg gcagcaggtt
    17581 ctgtcagaaa tttcatctgg aatcttttgt cagacaaatc cccttttgat catgaaacag
    17641 atatagatgt gattttcttt gatccagatt tttctgtatg aggaaacctt attactggag
    17701 aaaaagctga gagaggattt tcctcagtac cagtgggaat tgaaaaatca agtctatatg
    17761 caccagcaca gccctcatac tgctccctat accagttccc gtgatgctat gagtaagtat
    17821 ccagaacgat gtacggctgt tggactgcgc ttgaatgaag aatccgcttt ggaactcttt
    17881 actccatacg gtctggagga tattttgaat tttcaagttc gtccaactcc tcatttttta
    17941 gagaatcaag aacgaatggc tctctataaa acacgtttat ccaagaaaaa ttggcaggag
    18001 aaatggaaaa atttgatttt taaaaatact taaggaaact ttaagctagg gagtgtacac
    18061 taagttcata agttaagaag accttaactt aaactcctaa aactttttca taataatctc
    18121 cctataaaaa taaagtcgcc caatcaggcg gcttattttt ttgaaaaatg ggtatggtgc
    18181 ctgagaataa atagcttagt gatagaagaa aatggggaaa tatggtataa tgaaacgata
    18241 gatttttgaa taggaataag atcatgtttg gattttttaa gaaagataag gctgtggaag
    18301 tagaggttcc gacacaggtt cctgctcata tcggcatcat catggatggc aatggccgtt
    18361 gggctaaaaa acgtatgcaa ccgcgagttt ttggacataa ggcgggcatg gaagcattgc
    18421 aaaccgtgac caaggcagcc aacaaactgg gcgtcaaggt tattacggtc tatgcttttt
    18481 ctacggaaaa ctggacccgt ccagatcagg aagtcaagtt tatcatgaac ttgccagtag
    18541 agttttatga taattatgtc ccggaactac atgcgaataa tgttaagatt caaatgattg
    18601 gggagacaga ccgcctgcct aagcaaacct ttgaagcttt aaccaaggct gaggaattga
    18661 ctaagaacaa cacaggattg attcttaatt ttgctcttaa ctacggtgga cgtgctgaga
    18721 ttacacaggc gcttaagttg atttcccagg atgttttaga tgccaaaatc aacccaggtg
    18781 acatcacaga ggaattgatt ggtaactatc tctttactca gcatttgcct aaggacttac
    18841 gagacccaga cttgattatc cgtactagtg gagaattacg tttgagcaat ttccttccat
    18901 ggcagggagc ctatagtgag ctttatttta cggacacctt atggcctgat tttgacgaag
    18961 cggccttgca ggaagctatt cttgcctata atcgtcgtca tcgccgattt ggaggagttt
    19021 aggaggaaat atgacccagg atttacagaa aagaaccttg tttgcaggga ttgccctggc
    19081 tattttccta ccaattttaa tgattggggg cctcttgctt cagatagcaa tcggaatcat
    19141 agccatgcta gccatgcatg aacttttgaa gatgagaggt ctagagacca tgacgatgga
    19201 gggcctcttg accctctttg caacctttgt attgaccatt cccctggaaa attacctgac
    19261 ttttttgcca gttgatggga atgtggttgc ctatagtgtt ttgatttcaa tcatgttagg
    19321 aacgaccgtt tttagcaagt cttatacgat tgaggatgcg gttttccctc ttgctatgag
    19381 cttctacgtg ggctttggat ttaatgcttt actagatgct cgtgttgcag gtttggacaa
    19441 ggctctctta gccttgtgta tcgtctgggc gacagacagt ggtgcctatc ttgttgggat
    19501 gaactatggg aaacgaaagt tagcaccaag ggtatcgcct aataaaaccc ttgagggtgc
    19561 cttgggtggt attttaggag caattttagt aaccattatc tttatgatag ttgacagtac
    19621 agttgctctt ccatatggaa tttacaagat gtcagtcttt gctattttct ttagcattgc
    19681 tggacaattt ggtgatttac tagaaagttc gatcaaacgt cattttggtg ttaaggattc
    19741 tgggaaattt atccctggac atggtggtgt tttggatcgt ttcgatagta tgttgcttgt
    19801 atttccaatc atgcacttat ttggactctt ttaatcaaaa gacggaggaa acgctatgct
    19861 cggaatttta acctttattc tggttttcgg gattattgta gtggtgcacg agttcgggca
    19921 cttctacttt gccaagaaat cagggatttt agtacgtgaa tttgccatcg gtatgggacc
    19981 taaaatcttt gctcacattg gcaaggatgg aactgcctat accattcgaa tcttgcctct
    20041 gggtggctat gtccgcatgg ccggttgggg tgatgataca actgaaatca agacaggaac
    20101 gcctgttagt ttgacacttg ctgatgatgg taaggttaaa cgcatcaatc tctcaggtaa
    20161 aaaattggat caaacagccc tccctatgca ggtgacccag tttgattttg aagacaagct
    20221 ctttatcaaa ggattggttc tggaagaaga aaaaacattt gcagtggatc acgatgcaac
    20281 ggttgtggaa gcagatggta ctgaggttcg gattgcacct ttagatgttc aatatcaaaa
    20341 tgcgactatc tggggcaaac tgattaccaa ttttgcaggt cctatgaaca attttatctt
    20401 aggtgtcgtt gttttttggg ttttaatctt tatgcagggt ggtgtcagag atgttgatac
    20461 caatcagttc catatcatgc cccaaggtgc cttggccaag gtaggagtac cagaaacggc
    20521 acaaattacc aagattggct cacatgaggt tagcaactgg gaaagcttga tccaagctgt
    20581 ggaaacagaa accaaagata agacggcacc gactttggat gtgactattt ctgaaaaggg
    20641 gagtgacaaa caagtcactg ttacacccga agatagtcaa ggtcgttacc ttctaggtgt
    20701 tcaaccgggg gttaagtcag attttctatc catgtttgta ggtggtttta caactgctgc
    20761 tgactcagct ctccgaattc tctcagctct gaaaaatctg attttccaac cggatttgaa
    20821 caagttgggt ggacctgttg ctatctttaa ggcaagtagt gatgctgcta aaaatggaat
    20881 tgagagtatc ttgtacttct tggcaatgat ttccatcaat attgggattt ttaatcttat
    20941 tccgattcca gccttggatg gtggtaagat tgtgctcaat atcctagaag ccatccgccg
    21001 caaaccattg aaacaagaaa ttgaaaccta tgtcaccttg gccggagtgg tcatcatggt
    21061 tgtcttgatg attgctgtga cttggaatga cattatgcga ctctttttta gataatcgag
    21121 gaatattatg aaacaaagta aaatgcctat cccaacgctt cgcgaaatgc caagcgatgc
    21181 tcaagttatc agccatgctc ttatgttgcg tgctggttat gttcgccaag tttcagcagg
    21241 tgtttattct tatctaccac ttgccaaccg tgtgattgaa aaagctaaaa acatcatgcg
    21301 ccaagaattc gaaaagattg gtgctgttga gatgttggct cctgcccttc ttagtgcaga
    21361 attgtggcgt gaatcaggtc gttacgaaac ctatggtgaa gacctttaca aactaaaaaa
    21421 ccgtgaaaaa tcagacttta tcttaggtcc aactcacgaa gaaaccttta cagctattgt
    21481 ccgtgattct gttaaatctt acaagcaatt gccactcaac ctttatcaaa ttcagcccaa
    21541 gtatcgtgat gaaaaacgcc cacgtaatgg acttcttcgt acacgtgagt ttatcatgaa
    21601 ggatgcttat agtttccacg ctaactatga tagtttggat agtgtttatg atgagtacaa
    21661 agcagcctat gagcgtattt tcactcgtag tggtttagac ttcaaggcta ttattggtga
    21721 cggtggagcc atgggtggta aggatagcca agaatttatg gccattacat ctgctcgtac
    21781 agaccttgac cgctgggttg tcttggacaa gtcagttgcc tcatttgacg aaattcctgc
    21841 agaagtgcaa gaagaaatca aggcagaatt gctcaaatgg atagtctctg gtgaagatac
    21901 cattgcttac tcaagtgagt ctagctatgc agctaactta gaaatggcaa caaacgagta
    21961 caaaccaagc aaccgtgttg tcgctgaaga agaagttact cgtgttgcaa cgccagatgt
    22021 taaatcaatt gatgaagttg cagccttcct caatgttcca gaagaacaaa cgattaaaac
    22081 cctcttctac attgcagatg gcgagcttgt tgcagccctt ctagttggaa atgaccaact
    22141 caacgaagtc aagttgaaaa atcacttggg agcagatttc tttgacgttg ctagcgaaga
    22201 agaagtggcg aatgttgttc aagcaggatt tggttcactt ggaccagttg gtttgccaga
    22261 gaatattaaa attattgcag atcgtaaggt gcaagatgtt cgcaatgcag ttgtcggtgc
    22321 taacgaagat ggctaccact tgactggtgt gaacccaggc cgtgatttta cttcagaata
    22381 tgtggatatc cgtgaagttc gtgagggtga aatttcccca gatggacaag gtgtccttaa
    22441 ttttgcgcgt ggtattgaga tcggtcatat tttcaaactc ggaactcgct attcagcaag
    22501 catgggagca gatgtcttgg atgaaaatgg tcgtgctgtg ccaatcatca tgggatgtta
    22561 cggtatcggt gtcagccgtc ttctttcagc agtgatggag caacacgctc gcctctttgt
    22621 taacaaaacg ccaaaaggtg aataccgtta cgcttgggga atcaatttcc ctaaagaatt
    22681 ggcaccattt gatgtgcatt tgattactgt caatgtcaag gatgaagaag cgcaagcctt
    22741 gacagaaaaa cttgaagcaa gcttgatggg agctggttat gaagtcttga cagatgaccg
    22801 taacgaacgt gtcggtgtta aatttagcga tagtgacttg attggtcttc caatccgtat
    22861 cactgttggt aaaaaagcgg ccgatggcat tgtagaagtt aagatcaagg caactggtga
    22921 caccattgag gttcatgcag ataacgtgct tgaaacgctt gaaatcctaa gcaagaaata
    22981 aaaactataa tcagaagaaa aacaaggaaa aaatgtaact agtttttacc ttgttttttc
    23041 tgtataatgg gagaaatgag tagataaaga ggtaaatgta tgctaagatt tccaaaggat
    23101 tttgtctggg gatcctctac ttctggaccg cagacagaag gacgtgtagc tggtgacggt
    23161 aagggagaca atctctggga ttactggttc caagtggagc caaatcgtta ctataatggg
    23221 attggttcag ataagacatc gactttttat gaaaattggg agcgggatat tgagcttttg
    23281 ttagagactg gtcacacagc ctttcggact tctattcagt ggtcacggat ttttccacaa
    23341 ggctgtggaa aagtcaaccc tcaaggtgtg gatttttatc gtaaggtctt tgaggctatt
    23401 aaggctaaag gaattcgtct gttagtcaat ctctatcatt ttgatttacc ttttgccctt
    23461 caagaggatg gtgatggttg ggaaaataag gcgacagtct cagcctatga agactatgct
    23521 cgtttttgtt ttgagactta tggagattta gtggatcaat ggattacctt taacgagccc
    23581 atcgttcctg tagaatttgg ttatttttat gatgcccatt atccacataa ggtggatgca
    23641 gaggcagctg ttaaggtagc ctatcataca caattggcca gcagccgggc tgttaaggct
    23701 tgccatgaac ttttgcctga ttccaagatt gggattgtcc tcaacttgac accggcttat
    23761 ccacgtagcc agcatcctgc tgatgtcaag gcagctcgta ttgcggccct ttttcaggcc
    23821 caatctttct tagatccatc tgtcttgggg acttatccac aggagttggt agaaatcttg
    23881 catgaacacg gtcttttacc tgatgctaca gaggaagagt tggaactcat tcgtgataat
    23941 acggtggact tccttggtgt gaactactat caacctttgc gtgttatggc tcctcgattt
    24001 gctaagcatc cagatagtcc actcttacca gaacattttt acgagcctta tgtgatgcct
    24061 ggacgtaaaa tcaattctca ccgtggttgg gagatttatg agcaagggat ttatgacatc
    24121 gcccaaaata tcaaggaaaa ttatggcaat attgaatgga tgttgactga gaatggtatg
    24181 ggtgttgaag gggaagaaaa attccgtcaa gatggaatga ttcaagatga ttaccgtatt
    24241 gactttgtaa aaggtcatct tcgtgaactt caccgtgcca ttgaagatgg tgccaactgt
    24301 aagggctact tgatttggac ctttattgac tgctggtcat ggctcaacag ctataaaaat
    24361 cgctatggtt tggtcgaatt agacttggaa acgcaagaac gtcgtctgaa gaaatcaggc
    24421 cactggttca aagaattaag cgataataat ggattttaaa aaggactctg actgattatc
    24481 ccaatcggtc agagtttttt gcttacagga gcttaatttg aactatacca atttttactc
    24541 ttgacaagtg aaagaaacaa gtatatactg tttttgctga ttctataaaa gatgaattag
    24601 actataccaa ttttaaggag aaagcacagc ttgcctgtgt cgtatatact atgtgtggaa
    24661 ttgttggtgt tgttggaaac acaaatgcaa ctgatatttt gattcaaggg cttgaaaagc
    24721 ttgaataccg tggctatgat tctgcgggaa tttttgtcct agatggtgct gataaccatt
    24781 tggtgaaggc ggttggtcgt attgcagaat tgtctgccaa gacagctggt gttgagggaa
    24841 caactggtat cggacatact cgttgggcaa ctcatgggaa accaacggaa gacaatgctc
    24901 acccacaccg ctctgagaca gaacgttttg tcttggttca caatggggtg attgaaaact
    24961 accttgaaat taaagaagaa taccttgctg ggcaccactt caaagggcaa acagatacgg
    25021 aaatcgccgt tcatttgatt ggaaaatttg cggaagaaga agggctctca gttcttgaag
    25081 ccttcaaaaa agcccttcac atcatccgtg gttcttatgc ctttgccttg gttgactcac
    25141 aagatcctga agtcatctac gtagctaaaa ataaatcacc acttttgatt ggtcttggag
    25201 aaggctataa catggtctgc tcagacgcca tggctatgat tcgtgaaact aaccaataca
    25261 tggaaatcca tgaccaagag ttggtaatcg tcaaggctga tagtgtggaa gttcaagact
    25321 atgatggaaa tcgtcgtgag cgtgctagct acactgctga acttgacttg tcagatatcg
    25381 gtaagggaac ttacccttac tacatgctca aggaaattga cgagcaacca actgttatgc
    25441 ggaaactcat ccaagcctac acagatgagg ctggtcaagt tgtcgtagac ccagatatca
    25501 ttaaggctgt tcaagacgca gaccgcatct acatccttgc agctggaaca tcttaccatg
    25561 caggatttgc ttctaagaaa atgttggaag aattgacaga tacaccagtt gaacttggaa
    25621 tctcatctga gtggggctac ggtatgccac ttctcagcaa gaaaccactc ttcatcttta
    25681 tcagccaatc tggtgaaaca gcggatagtc gtcaagtttt ggtcaaggct aatgaaatgg
    25741 ggattccaag cttaacagtg acaaacgttc caggttcaac cctctcacgt gaagccaact
    25801 ataccatgct ccttcacgca ggtcctgaaa ttgccgtggc atcaactaaa gcctatacag
    25861 cgcaaatcgc agcccttgcc ttccttgcaa aagcagtcgg agaagcaaat ggtaatgcta
    25921 aagcgcaagc ctttgacctg gttcatgaat tgtcaatcgt agctcagtct atcgaatcaa
    25981 ctctttcaga gaaagaaacc attgaagcca aggttcgtga acttcttgaa acaactcgta
    26041 acgcctttta catcggacgt ggtcaagatt actacgtagc catggaagca agtctcaaac
    26101 tcaaagagat ttcttatatc cagtgtgaag gttttgcggc aggagaactc aagcacggaa
    26161 ccattgcctt gattgaagaa ggaacgcctg tcttgtctct cttgtcagat ccagttcttg
    26221 ccaaccatac tcgtggaaat atccaagagg tcgcagcccg tggtgccaaa gtcctcacta
    26281 tcgcagaaga gaatgtagca aaagataccg acgatatcgt ccttacgacc gtacattcat
    26341 acctctcacc aatttcaatg gtcgtaccaa cgcaattagt cgcttacttt gcaaccctcc
    26401 accgtggcct cgatgtggat aaaccacgta accttgctaa atcagtaacg gtagaataag
    26461 ctaaaaaagt ctagttatct aggctttttc taatgagcaa attggttgct tgtacttaag
    26521 attcgtgtta gaataatttt tcaaattgaa ggacagagat agacaaggag aatcacaatg
    26581 gtagaattgg gaatttcaac atttggggaa ataacggagc ttgaagggac tggacaaact
    26641 tacagtcatg ctgaacgcat tcgccagttg gtggcagaga ttgagctggc tgacaaggtt
    26701 ggtttggacg tatatgggat tggcgagcac catcgggcgg attttgcagt atcagcccca
    26761 gagattgtcc tggccgctgg ggcagtcaat accaagaaaa tccgtttgac cagtgcagtc
    26821 agcattctgt caagcatgga cccgattcgt ttgttccaac agtatgccac tatcgatgct
    26881 ttgtcaaatg gacgatcgga gattatggct ggaaggggct ctttcacgga atctttccct
    26941 ctgtttggtt atgacttgaa agactacgac tctctttttg atgagaaatt agacttgctt
    27001 cagttagtca atgaaaagac caagttagac tggcaaggtc gattgactca aacgattgct
    27061 ggtaaagaag tttatcctcg tccagttcag gacaaattgc ccttgtggat agctacaggt
    27121 ggtcatgtcg aatcaacagt gaagattgct caggctggtc taccgattgt atatgctatt
    27181 attggtggta atccacgtta ttttaaaaag ttgattcagg cttatcgtga gattggaagc
    27241 gaagcgggcc atgctgacaa agacttgaaa gtgggagccc attcttgggg gtggattgcg
    27301 gaagatggtg agcaggcggt gaaagattat ttccatccga ctaagcaagt ggtggatgct
    27361 atttccaaag accgtccgca ctggcaggaa ttgcgttatg agcaatattt ggagcaggta
    27421 gggtcaaatg gcgccatgtt tgttggcaat ccagatcagg tggccgaaaa attgattcgc
    27481 atgatagagg atttaggatt ggaccgcttc atgcttcatc taccgcttgg ttctatgcct
    27541 catgaccagg ttctgagagc tattgaactc ttcggaacac aagttgcacc caaagtacga
    27601 gcttattttg ctatgaaaga ggcttaataa aaaatcctaa tcaagtttat taggacaaca
    27661 aatattgtac gaaaattaat cccccgagct ataatgcttg ggggattttt ctataggagg
    27721 gctagtttag ttctcttttt tattttttag taagaaaccg agaccaagga gcgcaaggag
    27781 gctgattcct gcaaatagga gtttgttttc gtttttgatt cctgtatttg gaagttcagc
    27841 ttgtttatct ggggttgcag gtatattttc cttgctagag ttttctaccg attcgtttcg
    27901 aaccgcttct tttacagatg atgcttgtgc ttcttgtgct ggttgttcag cttgtgaatc
    27961 agctgtagct tggctaggtt tattttccgc atcagctact ttggcatctg gtttagtaga
    28021 atcaggtcta gcttctggag ctgggtcttg atgtgcaggg tgagaagctt cattttgatg
    28081 ttcaggcttg gaaggggttg agtctggttt ctcttctgca gttgactcat ggctagttcc
    28141 attttgagag actcgaagaa cagtagctgt aagggcattc aatttcaagc ctttttcagt
    28201 ccattcaagt cctttcgggt tggcaattcc gactggtcct gcttggtttt catctgccaa
    28261 aacttccgca tttcttagat gtgcaaaggc agttcccaaa ttaaattcgc gagctttttc
    28321 atccgcattg acaaagactg cgtagatatc gccgtttgga gcagtgattt ggtagccaat
    28381 cactacatcc tctttttcca caccattttg gcctgggaca gtgatgaggt ggacacggtc
    28441 tttgatatct tgaagactct taagtcggaa ggcatctgta gattgacgaa gggcaatcaa
    28501 acctttcata tagtcacggc tcttgacatt ttcaggataa gctttaccat ctgtagcctt
    28561 agtccagtca aacttgttga ctgcatcact agaatcgtaa gagtcatgga tgaagtaagg
    28621 atagtcaaat gggttgccgt ccttatcacg caacaagtga gatttgtttg gaaccttatc
    28681 ctctgctaca ggagttctgt aggctgggtc acggaattgt ttggtacgtc catattcctg
    28741 gccagagtgg ataaacggag ttccttgagc tgtcaagacc atgagatttc caagtctcaa
    28801 acgacggtgg atttcagcat agttctcagc cttgcttggg tcttttttga tagactgggc
    28861 aatgatgtca aagagggtca agttatcatg ggctgcgatg tattggatga catctccagg
    28921 gctgtcagct tcaaagttag ttggctgagc aatgagattt ttaaagatag tgttaacatc
    28981 acgcttgcca cctgtgataa aggcaggttg accttcgttt ggataaccag atttgaggtt
    29041 gttacggatg tcatctgaaa agacagcgac agtatcggta tgtttcatcc aatcttggtc
    29101 agcagcttta gtaggcatgt tttcatcacc ggcataggtt ctccaacctt cgcctaacat
    29161 gattagattt ggattgaggg cgcgtgcagc cttgtaagct tcttcgatag aagcggcgtc
    29221 atggtctccc atcatatcga aacggaatcc atccactttg taggtatcaa ctaggtattt
    29281 gatagagtca actaggagcc gtttggtcat atggtgggtt gtccccaagc gtccaccacc
    29341 aaagctagtt cgaggtgtgc catcggcatc cataaagtgg tagtagtttg gctctaggtc
    29401 ttcaaagata tcgactttgg ctgtgtggtt ataaacgaca tctaggatag ctcccatacc
    29461 acgtttgtgg atttcgttga tgaggttttt aaattctgcg attcgttttt ctggattctt
    29521 aggatcgctt gagtacatac cagtcaagga gaagtagttt tgagggtcat atccccagtt
    29581 gtagttgctg ttgcttgaag catagtcaga caagcgttca tggtttttca attcattgac
    29641 aaagtagtaa gacaagactg gaaggagctg gatatgggtt acacccaagt ctttgagata
    29701 gtctagtttt tcaatgaagg cttcaaaagt cccaaatggt ttggtcaagt cttttgcaat
    29761 ggcaggatct gaagtgaagt cacgcacatg agcttcgtag ataacggcgt cttcacgagt
    29821 cttgaaattg tgaatcttac cataagtcaa gtcttgaggt ccgagtttag ctggatctac
    29881 aaaggcggct ttagccactt tatgggcatc gtcaatcttg gcatcgtcgc tattccaagc
    29941 agcaagagat ttagcgtaag gatcgagtgc aagaacagtt ttaccttgac gctcgatttg
    30001 gtattgataa tagtagccag tgaaatctgt gattccgagt ttgtttgtgc tgtctagagt
    30061 ttgtttccaa gttcctcttt cccctttttc gagagcgaca gttccaacta ctttgtcagg
    30121 gtcattcttg tcgtagacaa caacagaaac cttatcagca cttggtgacc aaagggtcaa
    30181 atcaacttgt tttccttctt cttttaggtc agctcccagt ttgccatcat agctgtatgt
    30241 ctcatctttc aggcgccagc ttgttttggt agtgaattgg tcggaattgt agctaacagt
    30301 ataaggatgt tttgtgtcag agaaatctcc gctgtaggtc actttcttac cagcttcatc
    30361 gattgcaaca tcggtaatag ttaccttgtt tcctaggtga ttagtgatgt tggagtgttt
    30421 gaggatatct tcttttttag caccgacaag tgttgaaaag ctactttcaa tgctagaagt
    30481 gcctacgtgt tgggctcctg tcatacggat atcatggaca tagtatggat ttgtgtaaat
    30541 cgattcatca tcgtctttta ggaaaatttg gctatgattt ttcaaatctg tgaacttata
    30601 attttcttta cggattttca cgtcgtctcc ttgtttgctc tcatctagta ataaaaatcc
    30661 aaattctctt gcggcttcat taagaggaat gtcgatatag cggccatatt tgcctgtagc
    30721 cgtaaagtct gttccgtcag gccattgagc gctacttgga ttttttacat ctccccagta
    30781 ccagagagat ttcttgtcat agttgccatc tgtgcggtag tagttgacgc gaacagttcc
    30841 tgcaggctgt ggctcgtaag agaaaacctt gtaatcttgg tctaaccaag cctcgttcat
    30901 ttttggaact agtttttcta cagatttatc gccggttaga ttttttccag ctgtattgtt
    30961 gatgaggaag ctaattttct tggcttgttc tccctttaat ttgacatcta ggtaatagcc
    31021 gtagtcatct ttcttggcat ccttgaagga caaagctccg tttggccagt tttcagatgg
    31081 tttttcaaca tcgtcccaag tccatagtcc ttgagcatcc ttgttttctt caggaagttt
    31141 tttgacatgg atacggaagt agttatcttc gatgggctct tctgccttag tttcagttgt
    31201 tactggacta gtagaagtag ttggctctgt tgcaggtttt tcttctgttt tctctaggga
    31261 agcgtttgca tttctaggtg aagaaatatc actttcgttc ttaccagagc cagctgctgt
    31321 atcagtaaga ggttgggcaa gggtggttgt atttctgttg ttgggttcgt tagtggttgg
    31381 tgtactttcg tcggatgaga tagttggtgt agccatggca agcaagacaa ggcttgcgcc
    31441 gataaggaca gaaccagttc catttttgag ggaacggatg ctgtagacca tttttttctc
    31501 agtgtgagat ggtgtttttc tcataatgat tctccaatca ataaatttct atatcagtat
    31561 agcatgtagt aaaacaatat gcaagcgttt tctttgattt gtgaaaaaaa ttcttacttt
    31621 tgagaagttt tctgtactat tttatatatc agtataaatg acggagataa aaatgtcaca
    31681 taagccaagc cgtattatta ggaagatgat gtgttcccag ctatttttat tcagaaattg
    31741 atctctaaca ttaccaataa aaaagaaaaa tatcttgaca agcaagggaa aattttgtta
    31801 caataataga cggtactttt tacttttggt ctctcaagag tgtacaggga cgtgctgaca
    31861 aatgttgcaa aagtacacac agatgatatc tgtcaccaag tgtatcatca ccaaaaataa
    31921 aaaaacacag gagaatgtag atgcctacaa ttaaccaatt ggttcgcaaa ccgcgtaaat
    31981 caaaagtaga aaaatctaaa tcaccagctt tgaacgttgg ttacaatagt cataaaaaag
    32041 ttcaaacaaa cgtttcttca ccacaaaaac gtggtgttgc aactcgtgtt ggaacaatga
    32101 cacctaaaaa acctaactca gcccttcgta aattcgctcg tgtacgtttg agcaacctta
    32161 tcgaagttac tgcctacatc ccaggtatcg gacacaactt gcaagagcac agcgtggtgc
    32221 ttcttcgcgg tggacgtgta aaagaccttc caggggtacg ttaccatatc gtccgtggtg
    32281 cacttgatac tgcaggtgtt aacgatcgta aacaaggccg ttctaaatac ggtactaaac
    32341 gtccaaaagc ataaggaaag gggataaaga gaaatgagtc gtaaaaatag agctccaaaa
    32401 cgtgacgtat tgccagatcc gctatacaat tcacaactag ttactcgtct tatcaaccgc
    32461 gttatgcttg atggtaaacg tggtactgct gcttcaatcg tttacggtgc ttttgagcaa
    32521 atcaaagaag ctactggcaa cgatgcactt gaagtatttg aaacagctat ggaaaacatc
    32581 atgcctgtac ttgaagtacg tgcacgtcgt gttggtggtt ctaactacca agtcccagtt
    32641 gaagttcgtc cagaacgtcg tacaacactt ggacttcgtt ggttggtaac aatcgctcgt
    32701 cttcgtggtg aacacacaat gcaagaccgt cttgcaaaag aaatcttgga tgctgctaac
    32761 aacactggtg cagctgttaa gaaacgtgaa gatactcacc gtatggctga agctaaccgt
    32821 gcattcgcac acttccgttg gtaagatagg atgcgaaagc gttaagaaag tcccagagaa
    32881 aatagggaat cgaagcaggt tgcggttgca accaatgaga ttcatctttt tctccagact
    32941 tttagcttga gctcaactaa atcatgatgc taggaacggt aaggatgcaa ggtgaaaata
    33001 ggaaactgac gcagtattcg acgaatacaa ggagttttat ctttttcacg cagcatcccg
    33061 ttccagctca catcggctaa ctaactttag cccgggttca aattagctaa atcgattagt
    33121 attagctata actcagctta ccatctcgta agttgaaacc aacaatagca tgaaaacatt
    33181 gagaacgggt aggtcctgcc tatccgtttt tattaaaatc gtgttataat agaatagaaa
    33241 tcaaaaataa ataggagaaa caaacctcat ggcacgcgaa ttttcacttg aaaaaactcg
    33301 taatatcggt atcatggctc acgtcgatgc cggtaaaaca acaactactg agcgtattct
    33361 ttactacact ggtaaaatcc acaaaatcgg tgaaactcac gaaggtgcgt cacaaatgga
    33421 ctggatggag caagagcaag aacgtggtat cacgatcaca tctgctgcga caacagctca
    33481 atggaacaac caccgcgtaa acatcatcga cacaccagga cacgtggact tcacaatcga
    33541 agtacaacgt tctcttcgtg tattggatgg tgcggttacc gttcttgact cacaatcagg
    33601 tgttgagcct caaactgaaa cagtttggcg tcaagcaact gagtacggag ttccacgtat
    33661 cgtatttgcc aacaaaatgg acaaaatcgg tgctgacttc ctttactctg taagcacact
    33721 tcacgatcgt cttcaagcaa atgcacaccc aatccaattg ccaatcggtt ctgaagatga
    33781 cttccgtggt atcattgact tgatcaagat gaaagctgaa atctatacta acgaccttgg
    33841 tacggatatc cttgaagaag acatcccagc tgaatacctt gaccaagctc aagaataccg
    33901 tgaaaaattg attgaagcag ttgctgaaac tgacgaagaa ttgatgatga aatacctcga
    33961 aggtgaagaa atcactaacg aagaattgaa agctggtatc cgtaaagcga ctatcaacgt
    34021 tgaattcttc ccagtattgt gtggttcagc cttcaaaaac aaaggtgttc aattgatgct
    34081 tgatgcggtt atcgactacc ttccaagccc acttgacatc ccagcaatca aaggtattaa
    34141 cccagataca gacgctgaag aaattcgtcc agcatctgac gaagagccat ttgcagctct
    34201 tgccttcaag atcatgactg acccattcgt aggtcgtttg acattcttcc gtgtttactc
    34261 aggtgttctt caatcaggtt catacgtatt gaatacttct aaaggtaaac gtgaacgtat
    34321 cggacgtatc cttcaaatgc acgctaacag ccgtcaagaa atcgacactg tttactcagg
    34381 tgatatcgct gctgccgttg gtttgaaaga tactacaact ggtgactcat tgacagatga
    34441 aaaagctaaa atcatccttg agtcaatcaa cgttccagaa ccagttatcc aattgatggt
    34501 tgagccaaaa tctaaagctg accaagacaa gatgggtatc gcccttcaaa aattggctga
    34561 agaagatcca acattccgcg ttgaaacaaa cgttgaaact ggtgaaacag ttatctcagg
    34621 tatgggtgaa cttcaccttg acgtccttgt tgatcgtatg cgtcgtgagt tcaaagttga
    34681 agcgaacgta ggtgctcctc aagtatctta ccgtgaaaca ttccgcgctt ctactcaagc
    34741 acgcggattc ttcaaacgtc agtctggtgg taaaggtcaa ttcggtgatg tatggattga
    34801 atttactcca aacgaagaag gtaaaggatt cgaattcgaa aacgcaatcg tcggtggtgt
    34861 ggttcctcgt gaatttatcc cagcggttga aaaaggtttg gtagaatcta tggctaacgg
    34921 tgttcttgca ggttacccaa tggttgacgt taaagctaag ctttatgatg gttcatatca
    34981 cgatgtcgac tcatctgaaa ctgccttcaa gattgcggct tcactttccc ttaaagaagc
    35041 tgctaaatca gcacaaccag ctatccttga accaatgatg cttgtaacaa tcactgttcc
    35101 agaagaaaac cttggtgatg ttatgggtca cgtaactgct cgtcgtggac gtgtagatgg
    35161 tatggaagca cacggtaaca gccaaatcgt tcgtgcttac gttccacttg ctgaaatgtt
    35221 cggttacgca acagttcttc gttctgcatc tcaaggacgt ggtacattca tgatggtatt
    35281 tgaccactac gaagatgtac ctaagtcagt acaagaagaa attattaaga aaaataaagg
    35341 tgaagactaa tccgtcctca ctctagaagg aagtcactta gtggcttcct tttgtcttta
    35401 gaaaatacct ctaaatatgg taaaatagta gaagaataat gtgaggaaaa tgaatgtcaa
    35461 atagttttga aattttgatg aatcaattgg ggatgcctgc tgaaatgaga caggctcctg
    35521 ctttagcaca ggccaatatt gagcgagttg tggttcataa aattagtaag gtatgggagt
    35581 ttcatttcgt attttctaat attttaccga ttgaaatctt tttagaatta aagaaaggtt
    35641 tgagcgaaga attttctaag acaggcaata aagctgtttt tgaaattaag gctcggtctc
    35701 aagaattttc aaatcagctc ttgcagtcct actataggga agctttctct gaaggtccat
    35761 gtgctagtca aggttttaag tccctttatc aaaatttgca agttcgtgct gagggtaatc
    35821 agctatttat tgaaggatct gaagcgattg ataaggaaca ttttaagaag aatcatcttc
    35881 ctaatttagc caaacaactt gaaaagtttg gttttccaac ttttaactgt caagtcgaga
    35941 agaatgatgt cctgacccaa gagcaggaag aggcctttca tgctgaaaat gagcagattg
    36001 ttcaagctgc caatgaggaa gcgctccgtg ctatggaaca actggagcag atggcacctc
    36061 ctccagcgga agagaaacca gtctttgatt ttcaagcgaa aaaagctgca gctaaaccca
    36121 agctggataa ggcggagatt actcctatga tcgaagtgac gacagaggaa aatcgtctgg
    36181 tatttgaagg ggttgttttt gatgtggagc aaaaagtgac tagaacaggt cgtgttttaa
    36241 tcaactttaa aatgacggac tatacttcaa gtttttctat gcaaaagtgg gttaaaaacg
    36301 aggaagaggc ccagaagttt gacctcatca agaagaattc ttggctccga gttcgaggga
    36361 atgtggagat gaataacttc acacgcgatt tgactatgaa cgtacaggat ctgcaggaag
    36421 ttgttcacta tgagcggaag gatttgatgc cagaaggtga gcgtcgggtt gagtttcatg
    36481 ctcatactaa catgtcgact atggatgctt tgccagaggt cgaagagatt gttgcaacag
    36541 ctgctaagtg gggacacaag gcggttgcta tcacagacca tgggaatgtc cagtcctttc
    36601 cacatggcta taaggcggct aagaaagcgg gaatccagct gatctatggg atggaagcca
    36661 atatcgtgga ggaccgtgtc cctatcgtct ataacgaagt ggagatggac ttatcagaag
    36721 caacctacgt ggtctttgac gtggaaacga cgggactttc agctatctat aatgacttga
    36781 ttcaggttgc ggcctctaag atgtacaagg ggaatgttat tgctgaattt gatgaattta
    36841 tcaatcctgg gcatcccttg tcagctttta ctacagagtt aactggaatt acagatgatc
    36901 atgtcaaaaa tgccaaacca ctagaacaag ttttgcaaga attccaagaa ttttgcaagg
    36961 atacggtcct agttgcccac aatgctacct ttgacgttgg ctttatgaat gctaattatg
    37021 agcgtcatga tcttccaaag attagtcagc cagttattga tacgctggag tttgctagaa
    37081 acctctatcc tgagtataaa cgccatggtt tggggccttt gaccaagcgt tttggtgtgg
    37141 ccttggaaca tcaccacatg gccaactacg atgcggaagc gactggtcgt ctgcttttca
    37201 tctttatcaa agaggtagca gaaaaacatg gtgtgaccga tttagctaga ctcaacattg
    37261 atctaatcag tccagattct tacaaaaaag ctcggatcaa gcatgcgacc atctatgtca
    37321 agaatcaggt aggtctaaaa aatatcttta agctggtttc cttgtctaat accaagtatt
    37381 ttgaaggagt gtcacggatt ccgagaacgg ttctagatgc ccatcgagag ggcttgattt
    37441 taggttcagc ctgttcagag ggtgaagttt ttgacgtggt cgtttctcaa ggtgtggatg
    37501 cggcggttga ggtggccaag tattatgact ttatcgaggt catgccaccg gctatctatg
    37561 cgcccttgat tgctaaagag caggtcaagg atatggagga actccagacc attatcaaga
    37621 gtttgataga ggttggagac cgccttggca agcctgtttt ggctacggga aatgttcact
    37681 atatcgaacc ggaagaagag atttatcgtg aaattatcgt ccgtagtttg ggacagggtg
    37741 cgatgattaa ccgaactatc ggtcatggtg aacatgccca accagcacca cttccaaagg
    37801 ctcattttcg aacgactaat gagatgttgg atgaatttgc ctttttggga gaggaactgg
    37861 ctcgtaaact ggttattgaa aacaccaatg ccttggcaga aatatttgaa cccgttgaag
    37921 tcgttaaggg tgacttgtat acgcctttca tcgacaaggc tgaagaaaca gttgctgagt
    37981 tgacctataa gaaagctttt gagatttatg gaaatccgct gccagatatt gttgatttgc
    38041 ggattgaaaa agaattaaca tccatactgg ggaatggatt tgctgtgatt tatctggcat
    38101 cgcagatgct ggtgcaacgt tctaatgaac ggggttattt ggttggttct cgtgggtctg
    38161 tcggatctag tttcgttgcg accatgattg ggattacgga ggtcaatcct ctctctcctc
    38221 actatgtctg tggtcagtgt cagtacagtg agtttatcac agatggttcg tacggttcag
    38281 gatttgatat gccccataag gactgtccaa actgtggtca caaactcagt aaaaacggac
    38341 aggatattcc gtttgagacc ttccttggct ttgatgggga taaggttcct gatattgact
    38401 tgaacttctc gggagaagat cagcctagcg cccacttgga tgtgcgtgat atctttggtg
    38461 aagaatatgc cttccgtgcg ggaacagttg gtacggtagc tgccaagact gcctatggat
    38521 ttgtcaaggg ttacgagcga gattatggca agttttatcg tgatgcagaa gtagaacgcc
    38581 tcgctcaagg agcggcgggt gtcaagcgga caacaggcca acacccgggg ggaatcgttg
    38641 ttattccgaa ctacatggat gtctacgatt ttacgcctgt ccagtatcca gcagatgatg
    38701 tcacggctga atggcagacc actcacttta acttccacga tatcgatgag aacgtcctca
    38761 aactcgatgt actgggacat gatgatccga ccatgattcg aaaacttcag gatttgtctg
    38821 gtattgaccc taataaaatt cctatggatg acgaaggcgt gatggcactc ttttctggga
    38881 ctgatgtgct aggggtaaca cctgaacaaa ttggaacgcc tacgggtatg ttggggattc
    38941 cagagtttgg aacaaatttc gtacgtggaa tggtagacga aacccatccg acaacctttg
    39001 cggaattgct tcagctgtct ggtctgtccc acggtactga tgtttggttg gggaatgctc
    39061 aggatctgat taagcaagga atagcggacc tatcgactgt tatcggttgt cgggacgaca
    39121 tcatggttta cctcatgcat gcgggtctgg aacctaagat ggcctttact attatggaac
    39181 gggtacgtaa gggtttgtgg ctaaagattt cagaagagga gagaaatggc tatatcgaag
    39241 caatgaaggc taataaggtg ccagagtggt atatcgaatc ctgtgggaaa attaagtaca
    39301 tgttccctaa ggcccatgcg gcagcctacg ttatgatggc cttgcgtgta gcttacttca
    39361 aggttcacca tcctatttat tactactgtg cttacttctc cattcgtgct aaggcttttg
    39421 atatcaagac catgggtgcg ggcttggagg tcatcaagcg cagaatggaa gaaatctctg
    39481 aaaaacggaa gaacaatgaa gcctctaatg tggaaatcga tctctataca actcttgaga
    39541 ttgtcaatga gatgtgggaa cgaggtttca agtttggtaa attagatctc tactgtagtc
    39601 aggcgacaga gttcctcatc gacggggata cccttatccc accatttgta gcaatggatg
    39661 gtctgggaga gaacgttgcc aagcaactgg tgcgggcgcg tgaagaggga gaattcctct
    39721 ctaaaacaga actacgcaag cgtggtggac tctcatcaac cttggttgaa aagatggatg
    39781 agatgggtat tcttggaaat atgccagagg ataaccagtt gagtttgttt gatgagttgt
    39841 tttaaaaaat tgcttaataa tctattaaaa gaggctaacg tatatccaat agatttacat
    39901 tagctttctt ttttgttaaa atagtctatg gaaagagggt gagagtatgt caaagatgag
    39961 tataagcatc cgtctggata gtgaggttaa ggagcaggcc caacaggtgt ttagtaatct
    40021 gggaatggat atgacaacag ctattaatat tttccttcgt caggcaattc aatatcaggg
    40081 attacctttt gatgttagac tagacgaaaa tcggaagttg ctccaagtgt taacggattt
    40141 agaccaaaat cgtaatatga gccagtcttt tgaatcagtc tcagatttga tggaggactt
    40201 acgtgcttaa gattcgttat cataaacagt ttaaaaaaga ttttaagttg gctatgaagc
    40261 gtggtttgaa ggcagaatta ttagaagaag ttttgaattt tctggttcaa gaaaaagaac
    40321 atcctgccag atatcgtgat cattcattga cggcatccaa gcattttcaa ggagttcgtg
    40381 aatgccatac ccagccagat tggcttttgg tttataaagt agacaagtcg gaattgattt
    40441 taaatttgct gaggacaggc agtcacagtg atttatttta atctatttta agggggttct
    40501 catgaaacta agaatatttg cggaagataa gccggctaag aaggtatttg aatatcaatt
    40561 agaacttgct gatcgtacaa ttcttctatc gacagcactc ttgtcaggtg ctattgcttt
    40621 agcaggaatc ttttctgctt tgaaagaaaa ataaaaatag aaaagagaaa acagaatggt
    40681 tttaccaaat tttaaagaaa atctagaaaa atatgcgaaa ttgttggttg cgaacggaat
    40741 taacgtgcaa cctggtcaca ctttggctct ctctattgat gtggagcaac gtgaattggc
    40801 acatctaatc gtgaaagaag cttatgcctt gggtgcgcat gaggtcatcg ttcagtggac
    40861 agatgatgtg attaaccgtg agaaattcct ccatgccccg atggagcgtt tggacaatgt
    40921 gccagaatac aagattgctg agatgaacta tctcttggag aacaaggcta gccgtcttgg
    40981 agttcgttca tctgatccag gtgccttgaa cggagtggac gctgacaagc tttcagcttc
    41041 tgctaaagct atgggacttg ccatgaagcc tatgcgtatc gcaactcaat ctaacaaggt
    41101 tagctggact gtagcagctg cagcaggact tgagtgggct aagaaagtct tcccaaatgc
    41161 tgcgagcgac gaagaagcag ttgatttcct ttgggaccaa attttcaaaa cttgccgtgt
    41221 ctacgaagca gctcctgtta aggcttggga ggaacatgca gccattctca agagcaaggc
    41281 caatatgctt aataaggagc aattttcagc ccttcactac acagcgccag gaacagattt
    41341 aacacttggt ttgccaaaga accacgtttg ggaatcagct ggtgctgtca atgcacaggg
    41401 cgaagaattc ttgccaaata tgccaacaga agaggtcttc acagcgcctg acttccgtcg
    41461 tgcagatggt tatgtcactt ctacaaaacc gcttagctac aacggaaata tcattgaagg
    41521 cattaaggtg acctttaagg atggacaaat cgtagatatc actgctgaga agggtgatca
    41581 ggttatgaaa gaccttgtct ttgaaaatgc gggtgcgcgt gccttgggtg aatgtgcctt
    41641 ggtaccagat ccaagtccaa tttctcagtc aggcattacc ttctttaaca cccttttcga
    41701 tgaaaatgcg tcaaaccact tggctatcgg tgcagcctat gcgactagcg ttgttgatgg
    41761 agcggagatg agcgaagagg agcttgaagc tgcagggctt aaccgttcag atgttcacgt
    41821 agactttatg attggttcta accaaatgga tatcgatggt attcgtgagg atggaacgcg
    41881 agtacctctt ttccgtaatg ggaattgggc aaattaagga gataatatgt taggaagtat
    41941 gttcgttggt ctcttagtgg gatttttagc aggtgctatg accaatcgtg gagagcgaat
    42001 gggatgtttt ggaaaaatgt ttctcggttg gatcggagcc tttctaggtc acttgctctt
    42061 tggaacttgg gggccagttt tatcaggaac agctattatc ccagcgattt taggagccat
    42121 gattgtttta gctatttttt ggagacgagg aagttaattt cctaaatctg atactcaatg
    42181 aaaatcaaag agcaaactag gaaactagcc gcaggttgct caaagcactg ctttgaggtt
    42241 gcagatagaa ctgacgaagt cagtaacata tatacggcaa ggcgacgttg acgcggtttg
    42301 aagagatttt tgaagagtat gaaacgaaaa ggaggttggt cattgtacta gcctcctttt
    42361 gatatgatat aatagttcta tgagattaga taaattttta gttgcctgtg cggtgggaag
    42421 tcggactgag gtcaaaaact tgctcaaggc tgggcgcgtg actgtaaatg gtaaaaaaga
    42481 aaaatcagct aaattgcaga ttgatgaaaa aatagatgag attcgctttg atgggcaagt
    42541 gttggagtat gaagagtttg tctactacat gatgaacaag cccaaaggag ttatctcagc
    42601 gactgaggat cccaagcaca gaaccgttct ggacttgttg gatgacttgg cgcggagtaa
    42661 ggaagttttc ccagtaggac gcttggatat tgacacgcat ggtcttttgc tcttgaccaa
    42721 tgatggtcag ctggctcatg ttcttctttc gcccaagcgt catgtggaca agacttatct
    42781 ggcacaagtc aagggaatca tgacccaaga agatgtggag acatttgctg agggtattcc
    42841 tctcaaagac tttacctgtc aacccgctat actggagctt gtatccatag atacagaaaa
    42901 gaatcaaagc caaatccgtg tgaccattgc agaagggaag tttcatcaga tcaagcgtat
    42961 ggtgggctac tgtggcaagg aagtggtgga cttgcaacga ttgactatgg gaactttagt
    43021 attagatgag aacctggaac gaggggaatg gcgtcgcttg accaaggaag aattagaaat
    43081 tctccgcgct aatattattt aatttagttt
//
LOCUS       DAVLVZ010000011        43092 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000011 DAVLVZ010000000
VERSION     DAVLVZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.011"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(18..1277)
                     /locus_tag="VYN77_000551"
     CDS             complement(18..1277)
                     /locus_tag="VYN77_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1558056.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPEESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     gene            complement(1624..3372)
                     /locus_tag="VYN77_000552"
     CDS             complement(1624..3372)
                     /locus_tag="VYN77_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558057.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLVIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(3374..5098)
                     /locus_tag="VYN77_000553"
     CDS             complement(3374..5098)
                     /locus_tag="VYN77_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558058.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLSKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQKLWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFIILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(5156..6094)
                     /gene="msrB"
                     /locus_tag="VYN77_000554"
     CDS             complement(5156..6094)
                     /gene="msrB"
                     /locus_tag="VYN77_000554"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1558059.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKGSLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(6242..6313)
                     /locus_tag="VYN77_000555"
                     /pseudo
     CDS             complement(6242..6313)
                     /locus_tag="VYN77_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(6389..7258)
                     /locus_tag="VYN77_000556"
     CDS             complement(6389..7258)
                     /locus_tag="VYN77_000556"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1558060.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            complement(7260..8546)
                     /locus_tag="VYN77_000557"
     CDS             complement(7260..8546)
                     /locus_tag="VYN77_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1558061.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(8697..9434)
                     /gene="mecA"
                     /locus_tag="VYN77_000558"
     CDS             complement(8697..9434)
                     /gene="mecA"
                     /locus_tag="VYN77_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1558062.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(9634..10668)
                     /locus_tag="VYN77_000559"
     CDS             complement(9634..10668)
                     /locus_tag="VYN77_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1558063.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(10697..12130)
                     /locus_tag="VYN77_000560"
     CDS             complement(10697..12130)
                     /locus_tag="VYN77_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1558064.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            complement(12146..13132)
                     /locus_tag="VYN77_000561"
     CDS             complement(12146..13132)
                     /locus_tag="VYN77_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1558065.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(13134..14237)
                     /locus_tag="VYN77_000562"
     CDS             complement(13134..14237)
                     /locus_tag="VYN77_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1558066.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(14227..15072)
                     /locus_tag="VYN77_000563"
     CDS             complement(14227..15072)
                     /locus_tag="VYN77_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1558067.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(15351..16625)
                     /gene="psr"
                     /locus_tag="VYN77_000564"
     CDS             complement(15351..16625)
                     /gene="psr"
                     /locus_tag="VYN77_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1558068.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(16622..17470)
                     /gene="pheA"
                     /locus_tag="VYN77_000565"
     CDS             complement(16622..17470)
                     /gene="pheA"
                     /locus_tag="VYN77_000565"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1558069.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEGVTIPLQSQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(17467..17943)
                     /locus_tag="VYN77_000566"
     CDS             complement(17467..17943)
                     /locus_tag="VYN77_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1558070.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(17936..19219)
                     /gene="aroA"
                     /locus_tag="VYN77_000567"
     CDS             complement(17936..19219)
                     /gene="aroA"
                     /locus_tag="VYN77_000567"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1558071.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSASFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(19311..19649)
                     /locus_tag="VYN77_000568"
     CDS             complement(19311..19649)
                     /locus_tag="VYN77_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1558072.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(19660..20763)
                     /locus_tag="VYN77_000569"
     CDS             complement(19660..20763)
                     /locus_tag="VYN77_000569"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1558073.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLESIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITKNTDYTVVIK"
     gene            complement(20773..21939)
                     /gene="aroC"
                     /locus_tag="VYN77_000570"
     CDS             complement(20773..21939)
                     /gene="aroC"
                     /locus_tag="VYN77_000570"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1558074.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLLLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(21949..23016)
                     /locus_tag="VYN77_000571"
     CDS             complement(21949..23016)
                     /locus_tag="VYN77_000571"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1558075.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(23035..23889)
                     /locus_tag="VYN77_000572"
     CDS             complement(23035..23889)
                     /locus_tag="VYN77_000572"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1558076.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEAHLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            complement(23879..24556)
                     /locus_tag="VYN77_000573"
     CDS             complement(23879..24556)
                     /locus_tag="VYN77_000573"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1558077.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(24553..25716)
                     /locus_tag="VYN77_000574"
     CDS             complement(24553..25716)
                     /locus_tag="VYN77_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1558078.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(26401..28035)
                     /locus_tag="VYN77_000575"
     CDS             complement(26401..28035)
                     /locus_tag="VYN77_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558079.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMIICFSNIAIHFPFEEIPIYFSLMIAILLVIAFSTSLTAFYNVFYESK
                     DLISYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVDPFSTEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKEFFRMVLRYHLTLLGQGTGVVTVLFTSAFFPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(28016..28765)
                     /locus_tag="VYN77_000576"
     CDS             complement(28016..28765)
                     /locus_tag="VYN77_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558080.1"
                     /translation="MIEFQNVSKLYGDKKALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            28944..30398
                     /locus_tag="VYN77_000577"
     CDS             28944..30398
                     /locus_tag="VYN77_000577"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1558081.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(30488..33106)
                     /gene="alaS"
                     /locus_tag="VYN77_000578"
     CDS             complement(30488..33106)
                     /gene="alaS"
                     /locus_tag="VYN77_000578"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1558082.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNHNASQLSSKLVAIVADNAEVEAVSEGTTSLIFAETPFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(33128..33613)
                     /locus_tag="VYN77_000579"
     CDS             complement(33128..33613)
                     /locus_tag="VYN77_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1558083.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(33888..34127)
                     /locus_tag="VYN77_000580"
     CDS             complement(33888..34127)
                     /locus_tag="VYN77_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558084.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(34388..35458)
                     /locus_tag="VYN77_000581"
     CDS             complement(34388..35458)
                     /locus_tag="VYN77_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1558085.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGSEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(35455..36228)
                     /locus_tag="VYN77_000582"
     CDS             complement(35455..36228)
                     /locus_tag="VYN77_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1558086.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(36225..37031)
                     /locus_tag="VYN77_000583"
     CDS             complement(36225..37031)
                     /locus_tag="VYN77_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1558087.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(37012..38169)
                     /locus_tag="VYN77_000584"
     CDS             complement(37012..38169)
                     /locus_tag="VYN77_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558088.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(38330..39235)
                     /gene="murB"
                     /locus_tag="VYN77_000585"
     CDS             complement(38330..39235)
                     /gene="murB"
                     /locus_tag="VYN77_000585"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1558089.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(39347..40099)
                     /locus_tag="VYN77_000586"
                     /pseudo
     CDS             complement(39347..40099)
                     /locus_tag="VYN77_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
     gene            complement(40109..40840)
                     /gene="budA"
                     /locus_tag="VYN77_000587"
     CDS             complement(40109..40840)
                     /gene="budA"
                     /locus_tag="VYN77_000587"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1558090.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELEERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     VGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            40982..42253
                     /locus_tag="VYN77_000588"
     CDS             40982..42253
                     /locus_tag="VYN77_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1558091.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(42273..43088)
                     /locus_tag="VYN77_000589"
     CDS             complement(42273..43088)
                     /locus_tag="VYN77_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1558092.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
BASE COUNT        12747 a         9729 c         8210 g        12406 t
ORIGIN      
        1 ttttcgtaat ttttctttta aagttctact gtcaggactt gaccttgctt aacaacctgt
       61 tctccggcga tataaacatc atctacatca ctagatttaa ctgcataaac caggtgagac
      121 agcatatttt cctgaggttg gagatgaatt ttcccttgtg gttgaatgac cagaaaatct
      181 gcttgcttgc cgacttccag acttcctatc tgattttcca ttccaaggac cttagcccct
      241 tcgattgtca gtaccttgag agctgtttcg attggaaact ggctggcatc cccacttttc
      301 atcttctgaa gaagagctgc agtccttcct tcctcaaaca tatctagatt gttattggaa
      361 gcaaccgagt cagtcgcaat tccgactgct actcccgctt tttggagctg gataattgga
      421 gcaattcctg atgccagttt gaggttactg ataggattgt gggcgatagc cacttgagaa
      481 gatgccaagc gttcaatttc tctctcgttt aattcgaccc cgtgagcaaa tacggacgga
      541 tgatctaaat aacccagttc ttcaagaaaa gcaagggggc gtttgccgta tcgtttgagg
      601 ataattcctg actcctcctt ggtctccgcc acatggacat ggagcggaat atttagctct
      661 tttgccattt ccaaactcgc ttccagcaag tctctactgc agctatacgg agaatgaggt
      721 gctaccataa ccttgaaatt tggattttta tattttaaga tttcatctat gatggatcga
      781 gttctgctta tagtctcagc agttgtttct gtctctgaag aaaagagagt cggagaaaaa
      841 taacaacgca tcttggaagt tttcaccacc tgataaattt gctggatatc cacaccattg
      901 ggattataca tatcgttaaa ggttgttgtt cctgactgga gcatctctgt tagggcttct
      961 ttaaccgcat tggtagtcat gtcgggagta aactcagatt cttctggcca gatatagtca
     1021 ttgagccatt catggagatt gctgtcatct cggatccctc tcagacctgt cattgcagaa
     1081 tgggtgtgac aattgaccaa accaggcata atccaagctc cctgatagtc tataatctgc
     1141 tcagcttgtt ctaaaaacgc tggcttatct tgaccgacat agacgatttg agaatccttg
     1201 actgctaaga ttccatcaag ataaacatgg aaattttgat cacaagtcac gatatttaca
     1261 tgctgaaaga ctttcattat aggctccttt tctaaaagac aagttgtact ctcttcaaac
     1321 cacttcagat tcactttgcc gtaggtgtgg ttactgactt cgtcagttct atctgcaacc
     1381 tcaaaacagt gttttgagct gacttcgtca gttctatctg caacctcaaa acagtgtttt
     1441 gagctgactt cgtcagtctt atctacaacc tcaaagctgt gctttgagca acctacggct
     1501 agcttcctag tttgctcttt gattttcatt gagtattaca gtgaacaatt tttctagcta
     1561 ttgtaacaaa aaagtcactc gaaggcaact tttttcgtcc ataaactttc aaaagagaat
     1621 ggcttattca gaactaaaag ccgcagcttt ttgcatttgg taatacttac cctttctatc
     1681 catgagttct tgatggttac catattcaac aatatcacca tctactaaga caagaattaa
     1741 atccgcatcc tgaatggttg acaaacggtg agcaatgatg aagcttgtgc ggcccttcat
     1801 gagttttgca aaggcatcct gtaccagcac ttctgtccgt gtatcaatgg aagaagttgc
     1861 ctcgtctaag ataagaatct ttggaataac cagaaagact cgggctatgg tcaagagctg
     1921 agcttggccg acagagagag attctccagc attttccaac ttggtatcgt atccctgtgg
     1981 caactgttgg atgaaaaagt ctgcattagc tgctttggca gcagcaatta cttgctctcg
     2041 actggcttca ggattgccaa aggcaatatt atcatgaatg gtcccttgtg tgagccaggt
     2101 ttcttgaagc accataccaa actgctgtct caatgatact cgtgtataat cataaatgga
     2161 ttgcccatcc agcaagatat ctcccgagct aatgggataa aaacgcataa ggagattgat
     2221 aagagttgat tttccagcac ctgtcggacc aacgatggct atcttactac cagctggaat
     2281 atcgatagac aagtccttaa tcaaaatttt ttcaggatgg tagccaaaag agacatgttt
     2341 aaaggaaata gctcccttaa cttggtcact ggtcaagact tccttacctg tttcagccac
     2401 ttcagggcta tctaagactc catagatacg ctctacgcaa gccagagcac tttgcaactc
     2461 agctagcact gaagaaatat cattaaaggg cttggtgtat tgctgaacat agttcaaaaa
     2521 agtcactaaa cgaccgacgg tcaaggttga acccatcatg atacgataag ctcctactcc
     2581 agctaaaagg gcataaatga gtgcatttac aaagcgagtc gaaggattga ccgttgaaga
     2641 ataaaagatg gctgactgag aatagcctga gtagttgtca tgagcctcac gcaatctttg
     2701 gataaattct gtttgagcat tgaaggactg gattatagtc tgctggctaa gcgattcttc
     2761 aatcaactga gtctgaattc ccctcgtctc tgtttgcttc tggaagagat gataggatct
     2821 cttggcaata aagcgtgaaa tcaccatgga cagtggcgtc aacagcaaga ctaagagagt
     2881 catgaggaga tgaatttgga gcatggctag aatactaacc aaaatcatca aaacaccaat
     2941 gaaaaattgg ttaaaaatca tggtcaagcc agctgccaac tgttcgatgt ccgtggttac
     3001 acgactaacc atctctccac taccttgcct atctacaaaa gcaatcggta aacgatggag
     3061 cttatggatg attcgctctc gcaaatctct agtataagag aagattagac gattatagag
     3121 gagaggattg gcccattgta ccagagtatt tcctattacc accaagagca tctggagaaa
     3181 aatctgccaa aaaactggtg atgaaccagc cactaggact tggtcaatga cctgcccaat
     3241 cagaataggt aggtaaattg ataagccaac ttgggcaata gttcctagaa aggctaggaa
     3301 aaggaggaag ggatggtttg ctaaatctac ggctaaacgt ttgagcgtct ggtttacagt
     3361 ttgtcgtttc attctagtcc tcctttccat gttgggatgc attgatttca cgatagactt
     3421 ggctggattt catcaagtca tcgtgcttgc caacagctag caactcacct ttttccaaga
     3481 ggagaatctg gtccgccatc tgtaaagttg aggttcgttg agagatcaaa attaagctcg
     3541 tgtttggaaa attttctcta atagctttca agagcttgga ctctgtaatg gtatccagtg
     3601 ccgaggttgc atcatctagg atgataaacg gagcttggcg caagactgct cgggcgatag
     3661 acaacctttg tttttgtcca cctgagaaat ttcgcccccc tgcctcaact agggcatcca
     3721 aaagtccttc cttatcactg acaaaatcct tagcttgcgc aatctccaag gcctgccaga
     3781 gtttctggtc agatacttct tgattgaaac ctagagtcaa gttggaacga atggttcctt
     3841 taaagagttc gaccttttga ggtacatagg caatccaaga ccgccactgc tccaaattaa
     3901 gaggactacg tccattttga taaaggtcaa tgttcccctt gtctactgga taaagtccaa
     3961 gtaagagttg caccaagctt gatttaccag aaccagtccc cccaatgata ccaaggattt
     4021 gtccttgagt catatcaaag gaaatgtctc tcagagaagg ctgggccgca tcaggatagg
     4081 taaaagtcaa ttcttggact tgtaaaaccc gaccactggt aacttgcttt tgttctaatt
     4141 ctgaatggat gttttcggga gattccgcaa agacctcctc gattctcttg gctgagatat
     4201 aggactggtt gagggaattg atcagcatgg caagcttgac caattccacc aaaatctgta
     4261 agaggtaatt gataagagca atgagagcac cttgactgag cactcctcct tgaattgaaa
     4321 tatagccttg ccagataata acgagaagag ttccattgac aatcagatag gtcagaggtg
     4381 ttaataaact agaccagaaa cctgtctttt cttgtaatct agcataaact tggttaaggg
     4441 tttgaaaaat ctgtaactct cgtttttctt gaccaaaagc acgaataacc cgcatccctt
     4501 gcaattgctg gcgcgtttcc tgaaccagtt ggtccgtttt ctttctgaga ctactgtaga
     4561 gaggattgac caatcgagat agccctacaa tgacaatggt caaaatggca accatgacta
     4621 agaaccagaa agtcaactca gctgagattc gataagccat aaaaatggca ccaaaaacga
     4681 taatgggcgc tcgcaaaaag agacgcagga attgattgat accagtctga atctggtagg
     4741 tatccgaagt caagcgagtg accaaactag aagttgtcag acggtctctg ctgtccttgg
     4801 acaaggaaag aatatgacga taaagatcgt ttgtcaattc cttagcaaag cctactgctg
     4861 cctttgctga gtaaaattga gctatcaagg ccactaaaac gccaattact gcaaagataa
     4921 ggagcaggcc aatctgcatc cagagatgac cttgatctcc ctgaggtaaa gattggtcaa
     4981 caatcccagc aatcaccatg ggaaccaaga gctcaaaaac agcttctaac agcttgaaca
     5041 agggggctaa aattgattcc ttgatgtagg gtttgaagta agataatagg tgtttcataa
     5101 atcccttcta ttcatattct agaaatgaag aaagtgggaa gccccactct ctgttttatt
     5161 tgtttaagta aggcaataga tagccatatc ctgctttttc catctcatcc ttggccacaa
     5221 agcgtaaaga agcagaattg atacagtaac ggaggccgcc taactcccgc ggtccatctg
     5281 tgaaaacatg acccaagtga gcactgcctg aacgagaacg aacttcaatt cgctccattc
     5341 catggctcag atccttgtaa taatgaatca actctttgga aatcggacgg ctaaaacttg
     5401 gccaaccaca acctgaagca aacttatcct tggcaaaaaa gagtggctca cctgtcgtaa
     5461 tatctacata aatcccctct tcaaaggttt ggtcataggc attggtaaat ggagcctctg
     5521 tagcagcttc ttgtgtgaca cgataagact cttcagatag actgcccttc aacacctctt
     5581 gactaggctt ttcatagttt gctgcatcaa tcaatggctt atcagcatcg gtcacatcga
     5641 tatgacagta acctgaagga ttcttcctga gatagtcttg gtggtagtct tcagccagaa
     5701 tgtagtggcg taattgctcc acttctactg caatctttcg acccagcatg cgttcctgct
     5761 cctgtaccac tgtgtagata gctggcaaat ctgcttcatc ctgataataa atcccagttc
     5821 gatattggcg accacggtca ttcccttgtt gattgataga tagaggatcg ataactcgga
     5881 aataataaag taaaatctct ctgagtgaca cttccttctc atcgtaaatc acttggaccg
     5941 tttctgcatg gtctgtttcc ttgatcaact ggtaattggt cgtttcgact tgaccattag
     6001 cgtagccaac actggtttct agcactccag aaatgcgtga aaaatattcc tctaggcccc
     6061 aaaaacaacc acctgctaga taaatttctg ccatctttat ttctccttta tcacgttttt
     6121 aactaatact cttcgaaaat ctcttcaaac cacgtcagcg tcaccttacc gtatatatgt
     6181 tactgacttc gtcagttcta tctgcaacct caaaacagtg tcttgagctg acttcttcag
     6241 ttctatccac aacctcaaaa cactgttttg agcagcctgc ggctagcttc ctagtttgct
     6301 ctttgatttt cattgagtat aaacttcttt tcacaaaaag gatatgaaac cctctggtca
     6361 agagtttccc catcctatct tctattcttt attttgcttc tacacggaca ccttgggtat
     6421 caactctcaa gtcatgcagt tttcctttga aaggttgctt ttccaattct gccttaattg
     6481 ttggcatctt gtcatgagaa gccagaacca taactgtcgg cccagcacca gaaaggtagg
     6541 ttgcataggc cccattttct ttggtcactt gcttaatcat cgcaaattct cttaccaagt
     6601 cctgacgata gcgctcatgg aagaggtctc cctcgattgc ttgcccagcg gtcaccatgt
     6661 ctcctgccaa caaggcagca accgctacat tggcgataga acttgcagca acagcttcct
     6721 tataagacaa ttttttaggc aagacactac ggctgtcgcg agtacgtaat tcatagtttg
     6781 gaatgtaagc tagaaaatca cactctggaa agtctgctac gatagcagag acttgccctt
     6841 caacagaact tgcaataacg agattaccat aaatggctgg agccacattg tcaggatgcc
     6901 cttcaatctt ggtcgctaac tgcaattttt catggtctga taagttgagt tgacccagtt
     6961 ggttggctag ttcaatccca gcaacgataa ccgagctgga agaacccaaa ccgcgcgcca
     7021 aagggacatc actggtcatt ttcaagcgtc ttggttgcaa gtctggtaca atttgcaaag
     7081 cgattttgag caagagatta cgctcatcat gtggaatcca tttgccaatc tggtgttcaa
     7141 tcagccactc atctcgttct tcgcagacct caatttgaag atacttggtt acagctacac
     7201 cgaccgagtc aaaacctggc ccgatattgg cactggttgc aggtacaata atcttcatct
     7261 tattctccta gcaccttgaa ggtattcaag aggtcgaatt ctgaaacctt cttcaattca
     7321 gctgagacat tttcaagctg ggctttatta atcttgtgtg tgatgataac gacacgcgcc
     7381 ttgtcaccct ctttgccatc ttgaaggatt tgcttaaagg aaatatcttg agcattgaag
     7441 atttcagcca acttcaagac ctgacctttt gagtctggag ccaagattga gaaatagtag
     7501 tttgctttga catcttcagg atttgccaag accaagtcac ggctatattc gttgaagtct
     7561 ttgccaatgg taccatcatt caaacgacga acgatacgga caatatcagc tacaacactt
     7621 gttgcagttg gtttttgacc cgcacctggt ccgtagtaca tagactcacc aataccgata
     7681 gattctacaa agacagcgtt cattacgcca ttcacactag caagtgggtg cgctttcggt
     7741 aggaaggttg gagtcacttc tgcagcaata cctgaagaag tttcctcaat agaaccaacc
     7801 aatttcacta cgtaaccaag ctcttgagct acagctacgt cttctggtgt gatattgcgg
     7861 attcccttgt gggctacatc atcaaaggca atcttcatgc caaaggcaaa ttggctcaaa
     7921 ataaccatct tgtaggctgc atcaatccca tctacgtcat tcgtcggatc gctttctgca
     7981 aatcctagac gttgtgcttc cgcaagagca tcatcgtaag accagccttc ttccaccatc
     8041 ttggtcacca tgaagttgga agttccgttg actactccaa gcacgcgcgt aattttatca
     8101 gaagccaagg aatttgctaa agtacgaaga attggaatcc caccagcaac tgctgcttcg
     8161 tagtaaagtg ctaccttgtt agcttgagcg atttctagca attctgcgcc atggacagct
     8221 aaaaggtcct tgttagcagt aacaacgtgt tttccagctt ccaaggcacg agtgataaag
     8281 gttttagcag gctcaatacg ccccatcaat tccactacga tagtaatatc ctggtctgat
     8341 aaaatatcat ccacattggt tacaaagtta aagtcattcc ctgctgcaag caagcgattt
     8401 ttttcatctt catccttgac caatacctta gcaactttga tatctgaatg tgctgattga
     8461 ttgatttttc ctccattttc ctttaggagg aaaggcacac cacttgcaac ggtaccaaat
     8521 cctagtaaag caattttaac tgtcatcttt atctcctcga aattttctaa tatagccatt
     8581 ataacagaat tttgtgaaaa ttcctattat agtaaatcac tatttcagta taaaaagaaa
     8641 aaacgaatca gacgattcgc tcttcttaaa atctgaaaat agctttccag aaaggattag
     8701 ccgatttttt gcagattgag cactgcatcg tgactcatca agacttgacc atactcttgt
     8761 aagactgagc gactgatatc actatcgtct gcaaactcgc gcatacgggc caacagccaa
     8821 gctggatatg ggcttggatg attttcaata tccactaaaa tggtcaaata atagcgctcg
     8881 ttcattttgt agagttcaga agtttccatt tcaaaagtca ctgtcttggc aaaagctacc
     8941 aagtcagcca acttagcaaa agaaaggatg tagtagatgt aaggttcttt cttactctca
     9001 gcttcttgtt cagcctgctc ttgctcttct tccttggctt caacttgctc aagagattga
     9061 atggcttcga tatcatcctt ggttttgtct gcgatgcttt tttccagggt tttgataaat
     9121 tcatctggag acatttgagc caattcttcc atatctggca aatccgataa gtcttcaaaa
     9181 tctagatttt ggtcaatctt tgacttggtc acaaagacat ctaccttatc aggttttgga
     9241 gtcacacgga agctcaacat gcctgtatcc agaaagctat caggcatctc tagctcatcc
     9301 aagatagcat aaaagaactc ttctgttttt tcttgaggaa cgagaaagtc agcaatctcc
     9361 attccacgat ccatcaaatc ctctaaagac atcgtgattt ttaaagttgt atcactaatt
     9421 tgtttcattt tcattgctag taacctcata ctttcagttc tatctattat actagatttt
     9481 tacgatttta tcaaaagaag gctcctctat acggatagat tttccctagg gtctttctat
     9541 aggagactcc aagagaaaat ttctgcagac agatagaaaa agccttcaaa atcggctctt
     9601 agccgatttt gaagacctta tacatcagaa tacttataat ttaaaggttg ctacaccgag
     9661 gatagaacga tttaagtttc tgagaatttg aagactttgc tcaaatttct tataacgagt
     9721 cactccgtac tcttcaacaa gaaggactgt atctctttcc aaaagagatg atacatcctg
     9781 caaatctaca aaatgcattc cttttaaagc ttcttgactc tgtttcaatt tatctaggat
     9841 agctttattt gagctaacga tggtcaattc ctgtccagta tttttgtatg acaaaacatc
     9901 tgctaggtta gcaattgttg taatctctgt tacaaaatca atttgatact gagaaaaatc
     9961 acctactcta ttgattgttg gattaaagag ataaactaac acatttccca tcacaaccaa
    10021 aatcacacaa accactccaa taacaactaa acgaagaatc agatttttca catttaagcc
    10081 aagcgctgtt tcaccatttg cgttcaattc tttagagttg atggtttcca gtttttcaat
    10141 tttcacattt gcataagcat gtttaaattt ctcaatcaac ccatcaattt ttttctctaa
    10201 caagtcattg gcatctttac ttgatgtcaa aattttcaca ccaacccctg catcgtcaat
    10261 catatagtag acggtcaatt ttttccacca atagtcattc gttgaatttt tcaaggttgt
    10321 ttccgtcgtg tctaattcac tggcaatttt tttcaactca ctgggttcta catcattgaa
    10381 aagataagct ccattcaaat taccatcaat caatttccca taaaaatcac tataaccacc
    10441 aatttgatga ttcaaaatcg ttttggccga atcttctgga ggagtgattt tatagataag
    10501 ataagttgaa taacttgttg tatctttgac agtgttttta ttcctaactg ctttaattgt
    10561 aaatggtaca gcaatgagag caaataaagc gatgagagct aaaatatttg cttttcgttt
    10621 tttataaaga tttgcaaaca aatcagctac tgaataatgt tcaaacatga tttttttctc
    10681 ctttgtttag tagatactag ttttcctttg taagcatttt tgctacaaat ataatcacaa
    10741 gaacaattcc ccagaattgc attgtaaata aattgaagaa actttctgaa aagctgcttc
    10801 ttggcataaa gaatagatta ttcaagatga gtagggataa agcaaatagg attgtccttg
    10861 agcgataggc tacttgcagc atggctataa ataatacgcc gagtaagaaa ctaagcagaa
    10921 agactccaat cataccatag tcggtataca actccatgat ataactactt ccgataccat
    10981 gccctttcaa gtattccttg ttcaagacaa gataggatag attgtgggca taactattac
    11041 tatcaatagc tagttccaca ctattggttg tatgttcaaa ggcttttcct ccgaaaatgg
    11101 ctcccaaact cccccttgca aaataatcaa gaacaggacc aaaagtaaaa ttacggaaat
    11161 ctcggtaagg gaggctactg ttaaatagaa aacctcgagc cagaacacca aaactagtcc
    11221 cttgtttata gataaagtca agtaagatat cccagaaacc tgtatgagaa acttggacat
    11281 tatcccgtac ataattgagt actcccatcg ctaacatgag aataggagaa cctacaaaaa
    11341 tcgctaactt ttctttaaac ccaatccatt ttcctttttc agtttgctcc cgcataaagt
    11401 aataaacaaa agcaaataaa atacttaaaa taaagggatt tcgtgtccca attgccaaat
    11461 gaatagtatt agctgcaata aaggagacaa gcactgctgt ggcctgcaat ttctttggct
    11521 tggttgccag atacatacac attgcataga ccgtaaaggt agacaaaatg taggtaaaat
    11581 aaggcagttt actttcaaaa tttgcatagt aggcatagta ggaagtctgc aaacgataca
    11641 agagccgttc aaataaccga atgaaataga aaggataggt tagaagaaaa actcctagtg
    11701 atacaaagcg taaccgcttg atataaacct cttttagaga atttcctata tttgctactt
    11761 ttattttctt cctagctatg aagtaacgag ccagaatgcc tcctgtggtc aagcccagaa
    11821 tcgaaaccat gacaactata aaggcaaaac gataggctat tggatgatag gtatccaaag
    11881 caccatccct aaaataatca atggtcggtc ttgataccag aaatacaaaa atggttaaat
    11941 agaaaataaa atggattaag taatacttga tatcattcca acaagcaatt aagctactaa
    12001 ccaacaagaa caataaagta gaaagtaagc taacattatt attattaaac agatacacaa
    12061 ttccacttac tagcgtcaag gcataactga ctatggtcaa actaaataat aatcgtttcc
    12121 catcaatcac ttggtcaccc ccgttctaat gtaatttttt agatttttca atatttttca
    12181 gtaataagaa tcgatataag gaaatattta tgaatagggc caaagcacta attcttctcc
    12241 ccttacggaa aattggattc ctagaaatag caaaggcatg gccttttaaa aaacgatgaa
    12301 tctgagaata ggcttcaaac tgtttatact gatcatctag caacatctta tccagaataa
    12361 agaagtgggc ataggccaat ctgaaaaaag cgacctcttt caagtcagga tagtttttca
    12421 caacttcatt ataaaacttt tggtagatat caatataggc taaatccttc tctgcatagg
    12481 gtttggtcgt aatactatcc cctctatgga aatagtaata atagggttta gtattaacca
    12541 catacttctt ggccaacttg attaaatcaa aatggtaata ggcatcttcg taaatcaacc
    12601 ccttaggaaa ggatagggca gttgcaatct gtctcttgat tagcttattg caaatcgtcc
    12661 caggtatttt ttcacctatg aggtattcct ttagaaatgt ttgagaatca cagacaaaat
    12721 agtcatcctg attggctgac tgtgggcttt catcattagc atagacattc atgacaccac
    12781 agcttgaaac atccgcatct tcttgaacta attgctcata taagctctga atcatttctg
    12841 gatggatata atcatctgag tcaataaaaa tcagataatc cccgtgagcc tgcttcatcc
    12901 catcatttcg tgcttgcgac aatccttcgt tctttttatg aagcactgac accctgtcat
    12961 cttgttcagc gattgaatca cacaagcgac cactttcatc tgttgcacca tcatcaacaa
    13021 gaataatttc cagattttga taggtctgct tctgaatgga agctatcgat ttttctaggt
    13081 actgcgccac attatagact ggcacaatca cactaattaa tgcagtttcc atgctactcc
    13141 tctaatagtt tttctacttg ttcgatttgt tttgtaattg taaattgttg aatgaattgg
    13201 ctagcctcat cgacatcaaa gtttgaggca gaagtcatgt aattagtaat cgcctgagct
    13261 gcctcttgat tgctctcaat gatttgtcca aatcgtcctt cttgggataa ttcctcagcc
    13321 cctccaacgt ccgtagagat aaaagggagt cccagactca aggcctccac atacactcca
    13381 ggaaaacctt cttgtttaga catagacaag agaactttcg tctgagatag atactgataa
    13441 ggattttttt gataaccaag gaaatgtaca tagtcctcaa tcccatactc tttgactcgt
    13501 tttttcagtt cctcttccat atcaccagcc ccgataaaat agagatgata gttttttccc
    13561 tcttggtgta ataatcgtat cacttccact acacggtcag aacccttatt ttcctcaatc
    13621 cgtccgatag tacagatact ttgaggagca atctcgatat cgatcttctc ttgagatttt
    13681 tctagaatag tctgaaaatc atatccattg tagattgtct gtaatttaga agcataatct
    13741 ggataaactt ccttgataga attgctggtc ttttttgaaa tccctacaat tgtattcgca
    13801 gcatccaact ggcttctatg tgattctctt ttagagctat ccttaagaag ttcttcaata
    13861 cttccatgaa tccaagatat cttcttgact tctcttcttt tagagaacaa cagtggtgga
    13921 ttcataatgg taaaagaaac ttcaacatca taatcatctt ttacaagcaa acgacgagcc
    13981 agtcttggaa aataaattct cattctccac aaaaaagctc gtaaccatct ggtttggcga
    14041 taatcttgaa gggattttaa aatgcgtaca tgctttggaa cagattcata tcccttgtca
    14101 aagtgctcca tttcaagaat atcaatatca tacttttcta gatccagatt tgaaacaatg
    14161 gttgatagaa tcttctctgc accacctcca agagaaaaag accacataaa aaataagatt
    14221 tttttcttag ccaccatatt ctcccttgta ttctgtataa gacttatcca tatcagcgat
    14281 gacagcatca tgatgcggta cctgcttgtc tgctggtgga ggcgtcatat aatccccaaa
    14341 agcagttctg agatagacat catagccgat tggaataggc atctctgttc cttcaaatgg
    14401 caagaaaaga ttgtcttcaa aagatgtgat tgggtacttg tttctcatgt agccaggacc
    14461 tgagcataat tctgtaatgc catcacaatc agccaaatca tacttagtca tttctttctc
    14521 agcttttttc cagatgcgat aacggagaga ttttggagtc aaacccagta aaatgcgact
    14581 tccccatttc atgagagcac catgcttttc tggaatagtt tgcgcacaaa agagtgaata
    14641 aatcaaggcc caacgaacct gttttttccg ctcagctgga tctttcggat aataatccaa
    14701 aggcaaaaca tccaaggcca gaccatgtgg caaatccaaa tcctgctgat aaggcttgat
    14761 acaggtggtt tccttgtcac gaatggtaat aaaaagatta cgatcaacaa aatccttgta
    14821 actctttgac aagaaataac gttcatctgc ataacgaggc cataattctg ctaatttctc
    14881 ataatcttta cgaggcataa aaaagtctag gtcgtcgtcc caaggaataa atcccttgtt
    14941 tcgaagggta ccaatagcgc ctccgccaca gagataacag agcaaatcat gttctttaca
    15001 aaaggccaca aaatattcag ccatctccag actacgagcc tgaattgctt ttaaatcagt
    15061 catattgttc attattcttt ctatcgtatc gtttcattat accacaaaca aggggtgaaa
    15121 atctattgca gactgtaaaa aatcaaagcc tgactgctat ccaaatagct atcaaacttt
    15181 gatttttctg tcttatactc ttcgaaaatc tcttcaaacc acgtcagctt caccttgccg
    15241 taggtatagg taactgactt cgtcagtctt atctacaacc tcaaaactgt gtttttagca
    15301 gcctgcggct agcttcctag tttgcacttt aattttcatt gagtattatc ttatctcaag
    15361 cccatttgag cgagcttggt ttgatatttg ttttgatcaa ccagcaagcc caagcctcca
    15421 taaacatcat aggcatctac ccagtcaccc agttctggaa tcgtcaattt ttcaatacca
    15481 ttttttgctc catccaaaac agataaaccg tttgttagga ggaaagtata gggtacgttg
    15541 gttgaggtca tagcaaaaac ctttccaaga gcttcagaac cagtgaaaag tttagtggga
    15601 tctttaattt gctctaaaat tgctgttaaa acttgttgct gtctttttgt acggccgtaa
    15661 tccgcctcat catcatcacg gaaacgagca taattgagca gggtcgagcc attcatctgc
    15721 tgttttccga ctttaatggt ttgggttgga gactcagtct cggtagcata taaatcatct
    15781 ccgactgtag cttctgttag gggacgccca ttcaatgttg aaaattgagc atcaatcgtc
    15841 accccatcag ggaaaagcgt gtcaatcgct gtggcaaagg cctggaaatc aaccaaggcg
    15901 tagtacttaa tgtccaagtc aaaattatct ttcaagactt ggcgaaccat ttctgcccct
    15961 ttttgcccct cttgttctcc taactcgtag gctacgttta acttgttatc tgtctgtttt
    16021 ctaccattaa tcacttgact ataaccatct atatagacca aattatcacg catgaaactg
    16081 actagcttca ttttcttatc tgagcccccg acatttaata ccataataga gtcagttcgt
    16141 gtctcaacac tgttctggcc gattcgacca tcagtaccca tgattaaaat attaactcca
    16201 tctctagtgt cctgaccatt aaagacttct acttgagctg cccgggcatc agcagttttc
    16261 tttgcgctag catcttggta accacgcaaa aacatgaata ccatggccaa agccacacag
    16321 accaaaagtg aaaaaatcac cataaaaatt cgtttaagac ggagcttccg tcttttcttt
    16381 tttggaggga aagagagtgc ttgtgatttg gattgtgagc gactccggtt cgcatagctt
    16441 ggtaagtcaa cctgctcttc tctttcttgt tccaagctag agctactatt tcccctagca
    16501 agagttagct tttcttgcaa ataggcaaac tcattttttt ctctctcatt gagatagtga
    16561 atatttttta gcaaataatc ataacgcaac tgctcatgat gacttaaggg attttcttta
    16621 ctcatcttct ctcctttcca tggtctgata ttggataaat aggataggca cccagaattt
    16681 tatactggat tccaatcgct tctaattctt tttgggcaaa gtggaccaag tccttatcgg
    16741 tataatccac atcgataatg aaaaagtatt cacccagtgc tgtcttgagt ggacgacttt
    16801 caatttttgt caagtcaatt cctcgccaag caaaggtcga cagggcttta taaagtgcac
    16861 ctggaaggtt gtcaggtagg gtcaaggcta aactcatttt ttcagtttgt gattgcaagg
    16921 gaatagttac accttcagct cccaaaaccc aaaaacgtgt gaaattggct tccatttcct
    16981 gaatatcctc ggcaatcagt tccaatccat attcttcagc agaacttcta ggtgcaactg
    17041 ctgcaaaggg ctgatctgga tgttcggaaa taaaacgggc cgcataagct gtactagctg
    17101 ttacctcgat ttgagcctct ggatattgtt catcgatgaa tttctttcct tgagccaagg
    17161 cctgtggatg tgaaaaaatc ttttcaatct tagtatggcc tggaaccacc atcaactgct
    17221 gatgaatagg ctgaacgatt tctgctactg cttggatgtg agcctgatga aaaagatagt
    17281 ccaaggtttc atgaacacta ccctcaatag aattttcaac tggcaccaca gaatagttca
    17341 ctaatccttg ctcataagcc ttgatgacat ctgtaatgtt ggcaaaagcc tgcaattcct
    17401 catgaggaaa agctgtctgc acaacgtggt gtgaaaatga tcccttggga cctagataag
    17461 ctattttcat cttagttcct ctataatttc ctctgggctt agcttggtca catccaaaac
    17521 ccgactagcc acttcctcat accaagcctg tctttcttga aaaatagcta ctagttcttc
    17581 cttgctatta tttagaaaaa gcggtcgctg attgtcctta tcagctgcga tacgtaggta
    17641 gagggtttca aaatctgctt tcaggtagat gttatctgta ttagtcttga gtaagtcacg
    17701 atttctctga gaaataacca ctcctcctcc agttgacacg acttggtctg tttgtagtaa
    17761 atcagctagg acttctgatt ctacctgacg aaaggctgct tctccctttt cagcgaaaaa
    17821 attcgcaatg gacataccta ggcgcttctc aatcagagca tccatatcaa ggtaattagg
    17881 gtccaagcct cttgcaatag tcgattttcc agcccccata aaccctaata acaccttagc
    17941 catgaatcaa gctctccaaa tcatcaaaga aactaggata gctggtattg atggcttctg
    18001 cacggtcaag ctccacctct ccatctgcaa ccaagagggc tgcgatagct gtcatcatgc
    18061 cgatacggtg gtcaccaaac gtattgactc tagcaccgtg aagagctgat ttccctttga
    18121 taatcatccc atctgccgta ggagtaatat ctgctcccat actatttaag gcgtctgcca
    18181 caacctgaat acggtctgtt tccttgacct tgagctcctc agcatccttg ataactgtta
    18241 caccttgggc ttgagtcgca agcagggcaa taatgggcaa ttcatcaatc aatcgtggaa
    18301 tcaaagcgcc accaatctct gttcctttca agtcagaaga ctcaacaatc aaggtagcag
    18361 atttagcgac tggatcgatt tcagttattt ccaattttcc acccatggca cgaatgacat
    18421 caataatacc ggtgcgagtt tcgttgatcc ccacattctg cagcactaaa cgagaatttg
    18481 ggacaatcaa acctgcgact aaccaaaagg atgcactgga aatatctcct ggtacgacca
    18541 ccttttgtcc agtcagtttt tgaggtcctt gaactgtgat tttcttgcca tccacactta
    18601 aatggccacc aaattgttgc aacatatctt cagtatgatt acgggtgcac tctttttcga
    18661 taataactga ctccccctta gcttgtaagg ctgcaaacat caaggctgac ttgacttggg
    18721 cagaggcaat tggcaactca taatgaatag gtcttaggtt tttcgtccct tttaagcgaa
    18781 ggggaggcaa gtctcgttca gtttgccctg agatgctgac gcccattttt ttcagtggaa
    18841 gggtcacacg gtccatagga cgtttggaaa gactatcatc tccaaacatc tctacttcga
    18901 aatctgcacc agcaaggaca cctgaaatca ggcgaatcga ggtgccagaa tttcccatat
    18961 taagggcatt ttgcggcgct tttaagccag ccatgcctac accttgaatg gtaataaccc
    19021 catctttatc ctcaatttca acaccaaggt cacgaaaaac ctgcatggtc gaaagaacgt
    19081 cttcacctcg cagaatatca taaaccttgg tctcaccctc agccaaactt ccaaagataa
    19141 tggaacggtg gctgatagac ttgtcacctg ggacgcggat actaccatgt aaatggcgaa
    19201 tatttgtttt tagtttcata ctggacctca tacttgcaat acttttacct attttatcat
    19261 aaaaagccag aaattcctta aaaattcctg actttaggat agttcttttc ttatttcagc
    19321 aattctgaaa ctggttcgaa aacaattttt tcaatgtcag aaaggtaaat ggccaattgt
    19381 tgttgcttgg taaagaattc tgacaagagg ctatttcctt gaatctgttt accaaagcct
    19441 tccatcttag cttggaagga cgcatctggc atttgacctg tctgtgctag tttttgaatt
    19501 tcctcttgaa aggcaagata ttctgtaaag attttgcttg cctcagcatc tgctgcaatc
    19561 gcatctttag ctgctttaac agccttgtat tctggtaatc cgcgtagacc gcgactgagt
    19621 tcgtttgcac tatcgtaaat atttgacatg ttcttctcct tatttaatga cgactgtata
    19681 gtcggtattt tttgttatga gatgctcggc tctttccaag tcttgagcat ttttaaatga
    19741 aatttgtagg attccgtgaa tatcctcacg attttcctcg ttgatgtgga tgttaaccaa
    19801 ggaagtccca cgtagcagtt ccaaaatccg caggatgaca tcttcttcat caggaacgtc
    19861 aacatagagg tcgtaagagc tatccacacc accacgctta tggatttcca tggtctggcg
    19921 ttgttcacgc gcttggttaa aaaagttcca aatttgctct tcatctccct tactaatggc
    19981 ctgaccaatc gattccaaac gttccttgaa atcctcaatt ctatccagaa tggtctcgct
    20041 attggacaag agaatggagg tccacattcc tggctcgctt tccgcaattc gggtcatatc
    20101 tcgaaaacca cctgccgcaa agcgccttgc catctcatgc tcttgagcat agaccgcagt
    20161 ctgctccatg agactagaag ccaaaatatg aggaaaatgg ctaatctgag aagtgacacg
    20221 atcatgctcc ttggcatcaa tctcgataaa acgagcatga agacctgaaa gcagatcctt
    20281 catttcctta agcgtgtcct gacttgtcag gcttgaaggt gtaaagatat aataggcatt
    20341 ttcaaaaaga ttgacatctg ccgaagcagc ccctgtcttg tgactaccag ccatgggatg
    20401 ggccccgaca aagcgaacag acttgccagc caaatactgc tccgccgcat ccacaatggt
    20461 tgacttggtc gaaccagcat ctgaaataat aacgccttct cgcaaatcca aattggccaa
    20521 ctccttaatg aaagcaatag tttgtttgat tggcaagctg aggataatga catctgccaa
    20581 aggagcaaaa ctagcaaaat catccgttgc acggtcaatc atgccttctt tcaaggcgat
    20641 atctctcgaa gcttgactac gattataacc taaaatttca taatctggat gatcgcgttt
    20701 gataccaagt gccatagagg ctccaatcaa cccaagacct gcgatataga ttgtttttgc
    20761 cataggaact ccttaatagt tctttgtata gtctcggtgt ttggctaccg cttcttttag
    20821 ttcctcaaga ttatctgatg agaatttttc gaggatctct tgcgccagaa ccgttgctac
    20881 aactgcttcc atgaccattc ctgcagctgg aagagcagtc ggatcacttc tctccacggt
    20941 tgccttgtaa ggttcgtggg tttcgatatc cacactcata agaggtttat aaagagtagg
    21001 aatgggtttc atgaccccac gaacaacgat gggttgccca ttagtcatac caccttcaaa
    21061 accacctaga ttattggtac ggcgagtata accgtcttct ttagaccaga gaatttcatc
    21121 cataacttgg ctgcctttac gataaccagc ctcaaagcca agaccaaatt ccaccccttt
    21181 aaaggcattg atagagacaa cagcttgagc caatcttgca tccaattttc tatcccattg
    21241 gacataggaa ccaagaccaa ctggaactcc tccgacgact gtctccacaa ccccaccgat
    21301 ggtatcacca tcacgtttga tttggtcaat atagtccttg atttcctgtt ctcgttcttg
    21361 gttgacaata gaaacttcag actgggcagc tctttgctta atttcagcga ctgtcagatt
    21421 ttcaggaaca tctatttcct taccaccaaa gaccacgaca tggttggcaa tctccatatc
    21481 cagctcagcc aagaggcgtt tggctactgc accaactgcc acccgcatgg tggtttcacg
    21541 agctgatgaa cgctccaaag aatttcgcaa atcatcaaaa cggtacttaa tccccccaac
    21601 caaatcggca tgacctgggc gaggatgagt aattttccgc ttgcttttaa ggcggtcttc
    21661 aatgtcctcc gcagacatga tgtccagcca tttctggtgg tccttattga tgacatccat
    21721 agtaataggc gcccctgtcg tcttcccgtg gcgaacaccc gaagtaaaga caacctggtc
    21781 actctcaatc ttcatacgac caccacgacc gtagccaccc tgacggcgtc taagatcctc
    21841 attgatatcc tcagctgtca atagaagtcc agctggaatt ccctcaataa tagctgttag
    21901 acgggggccg tgtgattctc ctgcagttaa atatctcata cactctcctt attttaccaa
    21961 gtagtctttc atctcttcca gagaaactgg gtgaatggtc gctgaaccaa gctctggcac
    22021 caagaccaat ttcaaggtgt taccacgcgc tttcttgtca tgagtaagag cctgataaag
    22081 cttgtcaact ttccaatttt catagtcaac aggcaagccg aatttctgac acatctctgt
    22141 gatagattgg gtaatgccag ctggcatgag gcctttttcc tcagcaacct tggaaatctg
    22201 taccattccc atggcaacag cctctccatg catgaccttg ccataaccgg cagtcgcttc
    22261 gatggcatgg ccaatagtgt ggccaaaatt gaggtaaaga cgaataccat tgtccaactc
    22321 atcttcaacc accatcttgc gcttcacctg acaagaatgt tcaatcaagg tctctgcatg
    22381 ttccaaaata ctctcaacag aaccattcag tcccgtcaag agagcccaca gttctggatc
    22441 ctcaatcaag ccatacttga taacttcacc catcccttca atcaactctc tttttccgag
    22501 ggtttcaaga acaagtggat caatcagaac cccatctggt tgggcaaagg tccccaccat
    22561 atttttagca aatggtgtgt taacgcctgt ctttccaccg atagaagaat caacctgagc
    22621 tgtcaaacta gtcggaatct gaacaaagtg aataccccgc atataggtag aggctacaaa
    22681 tccagccagg tccccaacaa cgccaccacc aagagcaacg attccatcgc tacgagtcag
    22741 accttgcttg actagaaatt catagacttt ctgaacagta gttaaattct ttctttcttc
    22801 accttctaag aaatcaaaaa cagctacctg aaaaccagca tcttctaggc tgagcttgac
    22861 cttctctgca tagagagagg ctacatggtt atctgtcaca atgactacct tttgcggttg
    22921 ccagagttct cgcaaccact gaccagcctg ggccatacaa cctttttcaa tctgaatatc
    22981 ataaggatga tgaggaatat cgattctgat tttcatagga gagtctccct ttctttattg
    23041 gtatttttct gttaaagact gccaaatctc ttctgtcggc atttccttgc ctgtccacag
    23101 ttgaaaagct tctgcagctt gatagagcaa cattcccaga ccattgactg ctggattgcc
    23161 ctgacttcta gcccatttca aaaacggtgt ttcaaagggt tggtatatga tatctgcaac
    23221 taagagagtt tctggtaaga ctatgttttc aggaacagga gaggattggc catccatgcc
    23281 cacactggtg gcattaacta gcaaatccga ctcggcaatc cttgcttgca gttcagaaac
    23341 atattctaaa gcacacaaat ccactttaaa acctgtctgc tcctgtaact tgtctaggta
    23401 aggtcttgtt ttttccatag aaacggaacg aacaaagacc gaaatctgac tgacgccatc
    23461 caaaatagcc tgtgccaaga ttgatttagc cgcaccacct gcacccagca gggtcatctt
    23521 tttacctgaa attgtaaaag aaggcaagca cttaaaaaat cccttgccat ctgtattata
    23581 tccaattaaa ttgccattct cattgacaac cgtattaacc gcaccaatca agtgcgcttc
    23641 atcgctcagc ttatccaaat aaggaatcac ctgctcctta tagggcatgg acagattgat
    23701 gccaaacatc tggtagcgac gaatattggc cactgtttct gccaagtcac tcgcttcaat
    23761 ctcccaagcc acataagcac cgttggtagc tgtcgcctca aaggctctat tgtggatgaa
    23821 gggagaaata gaatgcttaa taggattggc aacaactgca gctaaacgtg tatagccatc
    23881 aagcttcatc caaaatctcc ctgatttttt tcatgctagc tagagaaatc tgcccagggg
    23941 cactaacctc atccagactg gcaaaagacc aactcgaacc agtcacatcc gcagtgatac
    24001 gagagacctt gcccacctta cccatagaaa tggtcacata ttcctgttca ggattgaggg
    24061 ttttaaagcc tcgtgtatag ttcatcaagt ctaagacatc ctgctccgtg tgagccatca
    24121 ccgcaacctt aacaagtttt ggatttagga tcgtcaactc tgacaagatt tccatcatgt
    24181 tctcaggtgt ttcttggaaa ttatggtaac tcaaaacaag atttgggaag tccagcattt
    24241 cctcaaaaac atccttgtag ctatagtact caaaatcaat atagtctggt tgatagagtt
    24301 gcgcaacttc cttgattaga tggatatact cttctggaga aaggtcgatt tctccacctt
    24361 cggagcgagt tcgtagcgtg aaaaccaact cacggcctgc gaatttttca aaaatggctg
    24421 gagctacctg caaaatcgct tctttaggca gatagtcggc acgccattca atgatgtcgg
    24481 catccaggta cctcgtggca tccagagcct gagcctcctc taaacttctt ggcattactg
    24541 aaacgattaa tttcatttac taaccttcat actaatcacc ttgaggtaat tactactttc
    24601 atctttttta ttataggcaa aatctgctgg aagaccatat ttgtttaaaa tctggtaact
    24661 tcttcctgca aaacctttat caatttgttc tgtaaatttc tgacgggaaa cattggcagc
    24721 attggtactg gcaatgataa tccctcccgg atttaaaatc tcaagactct gggaaatcaa
    24781 cttgtgataa tccttggcca cagagaaagt ttgtttttta ttccgagcaa agctaggcgg
    24841 atctaggaca atcacatcgt aggtcaagtc tttgcgtttg gcatatttga aatactcaaa
    24901 gacatccatg actataaaac gatgctcgtc tgtgctgagc ccatttgcct gaaaatgcgc
    24961 ttgagacaat tctcgtgaac gtttggctag atcaacagaa gttgtctggc tagctcctcc
    25021 catggccgca gctactgaaa aagccgctgt gtaggaaaac atattgagca aggatttacc
    25081 catagccaag ccgtcaacta aactaccgcg aacctcatgc tggtctagga aaattcctgt
    25141 catcaagcca tcattcataa agacttgata caggacacca ttttctaaaa cagtgaaaaa
    25201 gtcaggtgct tcttgaccat aaacatgggc agattcatag tccaaaccct taaagcggat
    25261 tttctcataa gctcctaaaa cctcagggaa aacctgtcta aaggcttctg atatagtctg
    25321 acgaatctga taaacataag agttatacca agaaaagacg gcgtagtcgc cataaaggtc
    25381 cactgtcaga cccccaaagc catctccctc ttgattaaag agacgaaagg cagttgtcaa
    25441 atcatcttga tagtaggcgt ttctcttttc tttggctttt ctaaacaacg tttcaaagaa
    25501 agcttgattg aaggccacct tgtctttgct gataaaccag cccaagccct tgttttgctg
    25561 agaaaggtag gcagtcccaa gaaagtttcc ttcctgaccc tgcacctcta cttcctgatc
    25621 cttaagattg acattctcaa gatcactggc ttctagtaaa actagcccct tagcaagctt
    25681 cttttcaacc cttttgctga ctcttattct attcataact accattatat caaactttta
    25741 gacaattctc aaaaaagaaa ctacccttgc ttttttactc ttcttttaaa aaatggtata
    25801 ctagacttcc tgcaaaacta ggaagtaaat gtgtaagaat cacagtaaaa aatgctcttc
    25861 cgtcttggag gagcatttct ttttatcaac gaaaatcaaa tagcaaacta tgaaactagc
    25921 ctcaggttaa ccgtgagatt ataggtagag aggttgtatc agcaatatgt gtctgtcaaa
    25981 tttagtgaca aaggtagtag aagaaagatg aagaaataaa tcagcttcag tagatatctg
    26041 gaaaatttga ttttatagaa aagccttttg ttacaaactc aatatactat caataaataa
    26101 tattatagaa gcaacaataa ttaaaatttc acctatttgc atcattctat ttcgaactct
    26161 aaatatatgt tctatcaaaa atacttggat cacacacatt ataggaatta acgtttttga
    26221 aattgaaaaa tatcccaata aataaactat aaacaacaat aatagaacta tattatattt
    26281 cttattcaaa acattcctcc ctatattttt gattaccaat cttaatcatt tacaactaca
    26341 ttctaacaaa ctataaaagc gtttgtcgaa ttgaatttat caagcaagca accaaccagc
    26401 tcatcttttt tctatttctg ccaatatacg tgacaggtaa taatgatagc caaaaatagc
    26461 aagagcaagc aagacgataa gagctcctac tcccaagttg atggcaagga taggggagag
    26521 agactgaacc aagaatatgc tcccaattac aagggccatc aggattgcac tataaataaa
    26581 caataaaact atggcgacta tgccatttga acgattcacc aggtccgtaa tgctactcca
    26641 attggttgac agatttttaa cgtccttaaa gtaatggtgg caagaaagga tgacactggc
    26701 aaggatccat gccacaataa ggtaaatcat cgaaatgatg ggcaagccta gatatagaga
    26761 aagaccaagc aaagtcagaa ctggtaaaaa ggactggaca gcatatataa tccaaaattt
    26821 cactttcaca taacgagcaa agtcaaaggg taaactctta agaaaatcaa cattttccct
    26881 ctccaaggac aaggcaattg aatgcaggct ggtgatattg ttattgacaa ctgctataaa
    26941 cactcctaca aaaaacaagg gtaaccagta tggaggatga atgtctggaa ctatctgaga
    27001 atctcggatt ttggaaatca gaccgatcat catgagataa ggaaagaaag cacttgtaaa
    27061 aagcactgta accacgccag taccctgtcc caagagggtg aggtggtagc gtaaaaccat
    27121 gcggaaaaat tcctttttag tggttgaaat tctctccttg ctgcgacgtt cttttttgac
    27181 cttctcctca ctattaagca ggatcacgtc ataaaaacga ggaaggacct tctttttggt
    27241 cagataaagc aggaagagag ttagtcctat ccaagcgagc agacccaata tggcttctgt
    27301 cgaaaaagga tccactgcta ttttgtaaaa gatatgaaga ggataaagga gaaatggaat
    27361 gtctctaact ttgtcaacaa tacttccaaa agtcgactga agaaagaaga taaatattaa
    27421 aggtatgaga actcctatcc caatcatcac attcgaaaaa atagactgat actttctgaa
    27481 gaccctagtt tgagccaaga aatgcactgc cactaccatc actagagcca cagagacaaa
    27541 taataaggtc aaggacagta gcatcaaagg caaacccagc catagagaag gagctagcct
    27601 aatgtagagg accagaaaat aagctaggat tggtacaatt ccagttagag ctggcaaaag
    27661 gacagacagt cctttagcaa ttataatctc tgattcttta aaggcatagg gcctatacga
    27721 tatcaaatcc ttactctcat aaaagacatt gtaaaaggcc gttaaagaag ttgaaaaggc
    27781 aatcactagt aaaatagcaa tcatcaagct aaaataaata ggtatttcct caaaaggaaa
    27841 atgaatggct atattactaa aacagatgat catcaagaga ctggaaaaaa tgtaagaact
    27901 taagactcta gcggaaacat ttactttttt cccaggattc ttagcctgct tctttcgtag
    27961 attagccaga ttagcttctt gagatgaata aaggatattg atatcaacta attttttaat
    28021 gaccttgaga cgcatccgca acctcctctt ttctaccagc aagactaagg tagatacttt
    28081 ccaaagactg gtctgggtaa tcttttctca agtcctctac actaccacaa taaatcaaat
    28141 gccccttttt caaaatggca atccgatcac agacttgctc tgccacctct aggacatgag
    28201 ttgaaaacaa gactgtcttc cctttttgtg catgttcctt catcatctgt ttcaaatcaa
    28261 aggcagcctg gggatccaaa ccagtcaagg gttcatccaa gacccaaata tcgggatcag
    28321 acaagagtgc tccgatgaca aagactttct gacgcattcc gtgagaaaga gtttcaataa
    28381 cctgatagcg attttcagca aaatcaaaaa cgttcaatag cctagctaga ctagcctcca
    28441 agtcagatct actcagatca taggatgagg cgatcaattc ccaaaattca ttggccgtta
    28501 agcgtaaaaa taagtcaggc gagtctgcta cgtagccaat ctttcgttta atagccaagc
    28561 gattttccga taactcctga ccgtctacca aaatacgacc actgctgggt gaaatgatac
    28621 tgactaggga ttttatagtg gtcgattttc cagccccatt atgaccaatc aagcccataa
    28681 tctctccatt ttcaatctgc aaattgagat tactcaaggc ctttttatca ccatacaact
    28741 tactaacatt ttgaaattca atcatgggac aactcctatc tttatctgta ttatatatcg
    28801 ctatactagc actttgatac aaaaaaatca atactttatt ttaagtagtt aaaatagttt
    28861 ccttctatct tacgcaaacg tttgcgctaa tagatacttt atgatagaat aaagaacaag
    28921 attgacaagt aagaggaaac attatgcaaa atcaaacact catgcaatac tttgaatggt
    28981 atctgcccca cgacggtcaa cactggacgc gtctggctga aaatgctcca cacctagctc
    29041 atctggggat cagtcacgtc tggatgccac cagccttcaa ggcaaccaac gaaaaagatg
    29101 tcggctatgg ggtctatgac ttatttgact taggagagtt caaccaaaaa gggactgtcc
    29161 gcaccaagta tggtttcaaa gaagactatc ttcaagccat tcaagccctt aaagcgcagg
    29221 gaattcaacc tatggccgat gtggttctca accacaaggc tgctgctgat cacagggaag
    29281 cctttcaagt tattgaagtg gatcctgtag accgtacagt tgaacttgga gaacccttca
    29341 ccatcaatgg ctggactagt tttaccttcg atggtcgcca agatacctac aatgacttcc
    29401 actggcactg gtaccacttc acaggtacag actatgatgc caaacgccgt aagtctggga
    29461 tttatctgat ccaaggggac aacaagggct gggctaacga ggaattagtc gataacgaaa
    29521 acggaaacta cgactacctc atgtatgccg acctagactt taaacatcct gaagtcatcc
    29581 aaaacatcta tgactgggct gattggttca tggaaacgac tggtgtagct ggtttccgtt
    29641 tggatgccgt taagcatatt gactctttct ttatgcgcaa cttcatccgc gatatgaagg
    29701 aaaaatacgg tgacgatttc tatgtttttg gtgaattttg gaacccagac aaggaagcca
    29761 atctggacta tctcgaaaaa acggaagaac actttgacct tgtcgatgtt cgtctccatc
    29821 agaatctctt tgaagccagt caagctggcg caaactatga ccttcgtggc attttcacag
    29881 atagcctggt tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac cacgataccc
    29941 aacgaggaca agcccttgag tctaccgttg aagaatggtt caagccagca gcctatgccc
    30001 tcattttgtt acgccaagac ggccttccat gtgtctttta cggagactac tatgggattt
    30061 caggccagta tgctcaagaa gatttcaaag aaatccttga ccgcctccta gccatccgaa
    30121 aagatttggc ctatggagaa caaaatgact actttgacca tgctaactgt atcggttggg
    30181 tacgttcagg tgctgaaaat caatccccaa tcgcagtcct tatctcaaat gaccaagaaa
    30241 acagcaagtc aatgtttgtc ggtcaagaat ggactaatca aacctttgta gatttacttg
    30301 gtaaccacca aggtcaagtt acaattgatg aggaaggtta tggacaattc cctgtctcag
    30361 ctagatccgt aagtgtctgg gcagtcaata ccatctaata gctcataata accaagctag
    30421 gtccaagcgg atttggcttt tttgtattca caaaaagacc tacccaaatg gatagatctt
    30481 tacttgatta caatttacct gctactgcat ccaacaattc ttggatctta ggttggttgc
    30541 ttcctcctgc catggccatg tctggtttac caccaccacg tccatcgatg attggtgcta
    30601 attctttgac aaggtttcct gcatgaaggt cttttgtctt gcttgctaca aggacattga
    30661 ctttgtcacc gatagcggca actaggacaa gaagatcaga gtagtctttt tgtttccagt
    30721 tatctgcaaa ggtacgaagg gcacctgcat cggatacaga cacttgacta gcaatgtaac
    30781 gatgaccgtt gacttcctta acatctttga agatatcgcc tgcggctgca gctgcggctt
    30841 tttctttcaa ctcagcattt tctttttgaa gttgacgaag ttgttcttga agtccttcta
    30901 ccttgtgagg tacttccttg acttgaggtg ctttcaaggt tgctgcgaca gctttaagag
    30961 catcctcttg ttcacgatag gcttcaaagg cttccttacc agtcactgcc aagatacggc
    31021 gagttcctga accgattcct tcttctttga caattttgaa gagaccaatc tcagaagtgt
    31081 tgccaacatg agtaccacca caaagttcaa tagagtagtc accaatagtc acgacacgaa
    31141 cttccttgcc gtatttctca ccaaagaggg ccatcgctcc catttcttta gcagtgtcaa
    31201 tatccgtttc aactgtctta acttcaagag cttcccagat tttttcgttg acttgctgtt
    31261 caatcgcacg caattcttca gcagttacag cttggaagtg ggtaaagtca aagcgaagga
    31321 attcaacttc gttaagagat cctgcctgtg ttgcgtggtt tccaaggata ttgtgaaggg
    31381 cagcgtgaag caaatgagtc gcagtgtggt ttttcatgac acggtgacgg cgattgctat
    31441 caattgccaa ggtatattct tggttcaagg caagcggtgc aaggacttca actgtatgaa
    31501 gggcttgacc atttggggct ttctgaacat tggtcacagt agccacaacc ttacctgact
    31561 catccaagat ttgtccgtgg tcagcgacct gtccacccat ttcagcatag aatggcgttt
    31621 ccgcaaagat aagtgaggta gttccttctg atacagcttc tacttctgca ttgtcagcaa
    31681 cgatagccac caatttagaa gacaattggc tagcattgtg gttgaagaca ctttctacag
    31741 tgatgttttg aagagtttca ttttgcatac ccattgagcc acccttgaca gctgacgcac
    31801 gcgcgcgttc ttgttgttct ttcatggctg cttcaaaacc ttcacggtct acagtcatac
    31861 cagcctcttc agcaatttct tcagtcaatt caactgggaa tccgtatgta tcatagagtt
    31921 taaagacatc ttgaccagca atgacagatt gacctttttc tttcaagtct gccacaatgc
    31981 cttgggcaaa gtgttgacct gagtgaaggg tacgggcaaa tgattcttct tcgctcttaa
    32041 cgattttctc aataaagtca cgtttctcaa gcacttctgg gtagtagctt tccatgattt
    32101 ttccaacagt tggaacgagt ttgtaaagga aaggctcgtt gatacccaat ttttgaccat
    32161 gcatagaagc acgacggaga agacgacgaa gaacataacc acgaccttca tttcctggaa
    32221 gggcaccatc accgatagca aatgaaagag aacgaatgtg gtctgcgata accttgaagc
    32281 tcatgttgtc gccatcttgg tcataaacct taccagacaa tttctcgact tcacggataa
    32341 tcggcatgaa gaggtccgtt tcaaagttgg tcttagcccc ttggataacg gccaccaaac
    32401 gctccaaacc agcgcccgta tcaatgttct tatgtggcaa ttccttgtat tcgctacgag
    32461 gaacagcagg gtctgcgtta aattgtgaca aaacgatgtt ccagatttcg atgtaacggt
    32521 cgttttcaat atcttctgca agcaggcgaa gaccgatatt ttctgggtca aaggcttccc
    32581 cacggtcaaa gaaaatctct gtatctggtc cagaaggtcc cgcaccaatt tcccagaagt
    32641 tgtcctcaat tggaatcaag tgacttggat ccactcccac ttcaatccag cggttgtaag
    32701 aatctttatc atctggatag taagtcatgt aaagtttctc agctgggaag tcaaaccatt
    32761 cagggcttgt caaaagctca taagcccaag tgatggcttc atcacggaag taatccccga
    32821 tagagaagtt ccccaacatt tcaaacatgg tatggtgacg cgcagtcttc cctacgtttt
    32881 cgatgtcgtt ggtacggata gccttttggg cattggtaat acgtggattt tcagggataa
    32941 tggtcccgtc aaagtatttc ttgagggttg ctaccccaga gttgatccac aaaagagttg
    33001 ggtcatttac aggaaccaaa cttactgatg gttctactga gtgacctttg gtcgcccaga
    33061 aatcaagcca catttggcgt acttgtgcac tagatagttg tttcatattg tctccttatt
    33121 tacttgttta atgtgattgg ctttccagca tttccacata gtcaatcgcg acacagaggg
    33181 aaatgactag gtctgcataa gcgtcttcaa gaaccgttac ggtataggta gaggtcagat
    33241 ggaagagttc cttcttaatt tccgcaatca actgatcgcg atcatccagc aatttgaaat
    33301 tcaaatccca gatattgccc tcgatacgaa gacctagatt atcaaactca tacttatctc
    33361 gccaaaaggt caacttctta cgaatgacaa aactcgagcc atcccgaagc tgaatctcaa
    33421 aacgaggaag caaggtcaag atttctttac tgatctgact gacttgttca ccagccgcat
    33481 catagatggt aaaagttttg ggaatcttaa aaaatgatcc ctccacctga taggcaattt
    33541 ctcccctgtc atccttgata gcgaagcgtt cgcctccaag acgaaacttt tgtttgacaa
    33601 gaaatgtttt catcaacacc tccaaaaatc aaaagacaag ctcatatcac gaagggcgaa
    33661 aaaccgcggt accaccttca ttcaatgaac ttgtcattct cttgttctta tgcaattgta
    33721 tgattgagta gcatgacttt ctagcttaga tggctcgcag caccgccatt tctctggact
    33781 aagacaagtg aaaattaatt ctcaactttc ttattataac gtttttttaa gcttgcgtca
    33841 actggaaatg atctccgttg aattagacca attccctaca tctctgatta ctttttcagg
    33901 atatattttt tcttactgcc gtttttcttt ttatcccaaa ttttcatatt actaaacaca
    33961 gctactagaa tatttccaaa tataaaggtg cctatcaccc aatatatgga ctcagttgtt
    34021 aggtattgtc gatccaagcc atcctttaaa tggaatagta taacagtttg gttaacaatc
    34081 ataaaggttg gccagaaact ttttttgaaa aaagtagaca ttttcattat ttgttgccgc
    34141 tttctgtaag gttaatactc aataaaaatc aaaaagcaaa ctaggaagct agcctcaagc
    34201 tgtacttgag tacggcaagg caacgctgac atggtttgaa gagtataggc ttagtatact
    34261 actaggcaag caaataagca aataagcaaa taaacaacta gaatagaaaa agatagggct
    34321 ctaaaaactg acttctattc cttaaaaacg aaccagcttg actgattcgt cttcttacgt
    34381 ttatctccta cttccgatac attttaaact gtaggaagag gtcgctatat ttccctgtcc
    34441 atttatggtc aaatttctca taaacttcta ggtgtttcat ggtttcaaca tcgggataga
    34501 aggccttatc ttcctttgtt tcctctggga gcaattcctt cgctggtagg tttggtgttg
    34561 aatagccgac atactccgca ttttggagag cattttcagg tttcaacata aagttgataa
    34621 aggcataggc tgagttttgg tttttaactg ttttgggaat gaccatattg tcaaaccaaa
    34681 gattgctggc ctctgtcggt accacataac gtagattttc atttttttct aacatttggc
    34741 tggcttcacc agagaaggtc acgccgattg caacattatt ctgaatcata tagcccttca
    34801 tctcgtccgc aacgatagcc ttgatatttg gagtcagttt gtagagctta tccactgtct
    34861 cttccaactg ctgcagatcc ttggagttga ggctgtagcc gagggaattg agtcctagtc
    34921 ccagcacctc acgcgcccca tcaaagagca tgatagaatt cttatactcc ggcttccaaa
    34981 ggtcatccca atgctcaggc gcttcatcta ccatggtttc gttgtagaca attcctaagg
    35041 ttccccagaa gtaagggatg gagaatttat tacctgggtc aaaggactgg ttgagaaact
    35101 ctgatccgat attttcgatt ccttcaattt ttgaataatc aagcggaacc aagaggtctt
    35161 cgtccttcat cttgttaatc atgtattcac ttggaatggc aatatcgtag gtcgttccac
    35221 cctgctttat cttagtgtac atggcttcgt tggagtcaaa agtctcgtac tgaacttgaa
    35281 ttcctgtttc ttctgtaaac tgagtcaaga gttcaggatc gatatagtct ccccagttat
    35341 agataaccaa tttttgacta tctcgactat tgattttact atctaaatga gtcgcaattc
    35401 cccacaagac aaggataatc gctgcaattc ctgctaaaaa tgaatagatt tttttcatgc
    35461 ttgctcctcc ttctcacgag agataaagta ataacctaca actaggataa tactaaagag
    35521 aaagactaga gcagacaggg cattgatttc taaggaaatc cccttgcgag cacgagagta
    35581 aatctcgact gatagggttg aaaagccatt tcctgttaca aagaaggtca cggcaaagtc
    35641 atctaacgaa taggtgaagg ccatgaaata accagtaatg atagacggag tcaggtaagg
    35701 aagcatgatt tccttgaaca tctgaaattg actagctccc aagtcatagg ccgcatgaat
    35761 catgtcgcca ttcatttcct tgagtcgagg caagaccatc aagaccacga taggaatgga
    35821 gaaggccacg tgactagata gaacggtcaa aaagccaagt gaaaacttga gttgggtaaa
    35881 gagaatcaag aagctagcac caatcataac gtcaggcgca accatgagga tattattgag
    35941 tgatagaaag gcttcttggt atttcttacg agactggtag atgtaaatgg caccaaaagt
    36001 cccgataatg gtcgctatca aggctgatag gaaggccaag aaaaatgtct gagccaaaat
    36061 cagcatgagt ctcccatctc caaacatggt ttcaaagtga gtccagctaa aacctgtaaa
    36121 gctattcata tcatcaccag cattaaaggc atagccaatc aagtaaaaga taggcaggta
    36181 gaggaccaga aagaccagtc ccagataaag gttggcaaat tttttcatcg ttctctcctt
    36241 tccttagtca cccacatggt gatgaacatg gtcaggatga gaatcacacc gatggttgaa
    36301 cccataccat agttgtcatt ggttagaaaa ttctgctcaa tagccgtccc caaggtgata
    36361 acgcggttcc caccaatcaa acgggtcagc atgaagagac tcaaacttgg gataaagacc
    36421 gactgaaccc cacttcgcac accgttcata gatagaggga agatgacatg acggaaggtc
    36481 tcccacttag ttgcaccaag gtcataacta gcattgatga gattattatc catatcgtct
    36541 aagacattga aaatcggcaa aatcataaag gggagctcga tgtagcttgc gacaaagata
    36601 aaggaaaaat cggtaaaaag caactgttgt gaaccaattc cgataaattc caagaattgg
    36661 ttaatagagc cattttgacc aaaaatcccg ataaaagcat aggctttaag gagcaaattg
    36721 atccaggtag gaaggataat cagcatgagc cagagttgac ggtgcttgag acgggtcaaa
    36781 aagagggctg ttggatagct gataagcaag gtcacaaagg tcacaatccc tgcataaagc
    36841 actgagttga aactcatttt gaggtaggtc aagttttgtg acgcaaagta agatttataa
    36901 ttttctaaac taaactggcc ttcaatgttg aaaaaggatt gtccgaaaat caagaccaag
    36961 ggtgccaata caaagagggc aatccaaagc atgtagggca ctacaaagag tttagagctt
    37021 gttttcttca tctctttcct cctcgatcgc attgatcaga cctgcttctt gctcttcgat
    37081 ttccacgtat tcttcgatac gagcatcgaa ctcttcttcg gtttcattga gacgcatgat
    37141 gtggatgtct tctgattcaa agtccagacc gatttcctca cccacgatgg ccttacgggt
    37201 tgagtggatc atccattcat ttccaagttc gtcataggcg ataatttcat aatgaactcc
    37261 acggaagagc tgggtgtcga ccttaacttg gagtttgcct tcttcaggaa gggtaatgcg
    37321 caagtcctct ggacgaataa cgacctcaac aggttcattt ggcttcatcc caccatcaac
    37381 cgcttcaaag cgtttgccgt taaattcgac caagtagtcc tcaatcatgg tacctggcaa
    37441 gatgtttgac tccccgataa aggtggcaac aaagtggttg attggctcat cgtagatgtc
    37501 cacaggggtt ccagactgga caatctcgcc atcattcata acgaaaatcc agtcactcat
    37561 ggcaagagct tcttcctgat cgtgagtgac aaagacaaag gtaatgccca atcgttgttg
    37621 taattcacgc aattcgtact gcatgtctgt tctcaatttc aagtccagcg ctgataaagg
    37681 ctcgtccaac aagaccacac ggggttggtt gatgatagca cgggcgatgg ccacacgctg
    37741 acgttgtcct ccagaaagtt tgcggatgga acgtttttca taaccttcca actgaaccat
    37801 cttgagaact tccgctacgc gttgctcgat ttctttcttg tcaatcttgc gcaagcgaag
    37861 tggaaaggca acattttcaa acacattcat atgtgggaac aaggcatagg attggaagac
    37921 agtatgtacg tcgcgcttgt tggttggaat atcattgata cgaacaccgt ctagcatgat
    37981 atctcctgtc gtcgcatcca gtaaacctgc aataatgttt aggatagttg atttccccga
    38041 accagatgca cctagaaggg tgtagaattt cccttcttcc aactcaaagt tgatgtcttt
    38101 gagaaccttg gtgttgctgt cttcaaaaac tttagagacg tttttgaatt cgataattgg
    38161 ttttttcaat tggcataaat tccttctttt tcatagatta accgatcggg gctctgtcag
    38221 gtccccacta cctcttgcag ggagtaaaac cacctgcata catcttcgct accgataggc
    38281 tttcaccccc ctttccatga catagcagca gcttttcaaa tacagcttcc tacttgcttt
    38341 cacccaagat ccggacttct ctttcaagcg taatacctga gtgttccttg actttttcga
    38401 taaccgattg gatcaagtcc tcgtagtctt tggccgttcc atctgcgaca ttgatcataa
    38461 atcctgcatg cttttctgac acttctacgc caccgatacg atagcctttc aagccagctt
    38521 ctgaaattaa ctgacctgca aaatgcccga ctggacgctt aaagaccgag ccacaagatg
    38581 ggtattccaa aggttgcttg agttcacgta ggtgcgtcaa gcggtccatt tcctgcttga
    38641 taacctgatg ggttcctggg gctagggcaa atttaactga caagacaact gcaccagact
    38701 cctgaatagc tgaatggcgg taaccaaaag ccaagtcttt agcagacagg gtttcgattt
    38761 ctccatcctt ggtcaagacc ttacaagact gcaatatgtg agcaatctcg ccaccatagg
    38821 cacccgcatt cataaagaca gcaccgccaa cgcttcctgg aataccacaa gcaaactcaa
    38881 agccagtcaa actatgacgg agggcaatgc gagttgtttc aatcaagtta gccccagctt
    38941 ctgcttcaat ggtatagcca tcaacagaaa cgttattgag cttgtcacac aagatgacaa
    39001 atccacgaat cccaccatca cgaacgatga tattgcttgc attgccaaga accatccaag
    39061 ggatattttc ttggttggca aatttcacaa ggcgagccaa ctcaaaacga tttcgtggaa
    39121 agaccaaata atcagcctct ccacctactt ttgtataact atagctatgc aagggttcct
    39181 gaaaacggat atcaattcct tctaagattt caagcatttt ttctcttaca gacatgtcac
    39241 tcttcctttt acaaaattca ttccattata ccatttttag agacatttga cgaccataaa
    39301 aataccttgt ttggattttg cataagaaaa agaggttccc ctctttttat gattttttac
    39361 aaaagatttc cttggttcca taggcgacca gaacgagctc cagtgctaga atcacttcaa
    39421 ccaagactgg atttgtcaac cagcctactt ggaaaagaga tggtgccaga tcaaagaagg
    39481 catgcaagcc ataggctgtt aggagataaa tccatttctt ctggcgaaca gcttgataaa
    39541 cccaaactgt caaaagtaat tggaaaccaa gcgccaagat tcgctcaaaa ccaagcaaat
    39601 aaatctgcca gaccgaaagt gactgaatgg tttttaacat attttcagac agtaattgca
    39661 taacctgtgg attctgagtt tgaactgccg aaagaacaat gtaaagattg agtaaactag
    39721 taaggcctag aaaaatcaac tccaagccac catgccccaa tccataggcc aaggcgtctg
    39781 ccttttccaa acttctcttt ttctccaacc atttgaagaa aacaagacga gccgtttcct
    39841 caaaaaatgc taccatggct aggccataga taatatagat aagcggatga acttacaaga
    39901 gggcaatact accgtctttt tgaggatgca aaatcaagat atgcaccagt ttttccaaaa
    39961 tctgcgaaga gacaaagaaa gcgactgcac ccaagtccaa gacagctaga ttaatctggt
    40021 atttcttttt ggcaaaccaa atgcttccta tcagaaaagc tagcaacagc aacatggtaa
    40081 taatgatatg aatggtcatt ttcttctcct attctgcctt ttcaatatct tttttcatct
    40141 cgtcaacatt gaacttagca aacaagtatt gacggtcttg gactgggaaa cgttggtcca
    40201 actggtcaac tgctcccacc tcgataatgc cttccttgat gacaaaatcc atgacatgtc
    40261 caccgaaggt caaatcatct gatatgaagt gcagatggta gcctgccaca ctgaccccat
    40321 ggaaaatctc aggcgtccag aaaccaacaa tagtccccac aacattgtca cgactatatt
    40381 ccggttgatg ggttgcgaca tcagcaaact tggtatcggg tgttgactta ggaatcatac
    40441 gtacatgcat atgtgaaaat tccccccgaa tcttgataga gcggaaaaga ttttccccat
    40501 cataataaga ctcaattcgt tcttccaatt ccttgtctgt catctcaaag cgctggcgga
    40561 aaatgacctc tgcctgatgc ggtactactg ctgcataagg aataagggca tctggtgaca
    40621 cttccacaat ttctggctgg tctcctgacc ctttggcctg ataagccttg ccatccaaga
    40681 caatcaattc cccatcaata gaatccaagg ttcccaaacc aaggtcacca tgctctagca
    40741 attctcccac tgtcatggta ccaccataaa ggccagccat caaggctcca agagtattgt
    40801 attgaaataa tttcactggt tcctgcacgt tcttatccat tcactttctt gtctgttatt
    40861 ctataaatct tactcctaag tataccacat ttgcccctag atgtgaacga gagaaacgct
    40921 ctagacattg ccaagaagga aaaaaaaggg tacaatgtaa caaaatcaag ggaggtctgg
    40981 aatgaagaaa caaagcaagt acaaagaggt cgtttcctat ctgaaaaatg gtatcgagtc
    41041 tggacgattt ccgacgggta gtcgcctgcc ttctatccgt caactgagcc ttgactttca
    41101 ctgcagcaag gacaccattc aacgagccct gctggaatta cggcacgaac aatatctcta
    41161 tgccaagcct cagagtggct actatgtatt agaacaaggg caacatcaag acctagaaat
    41221 cgaggttacc gacgaacatg ccagtgccta tgacgatttc cgactctgtg tcaatgaaac
    41281 cttgattggc cgagaaaact acctcttcaa ctactatgac aatcaagaag gattagaaga
    41341 cctaagacag tccattcaca aactcctctt tgagcaagct ctctactgca aggctaacca
    41401 actagtactg acttctggaa cccaacaagc cttgtttatc ctctctcaaa tatcctttcc
    41461 tagacaagcc aaggaaatct tggtggaaca gccaacctac catcggatga atcgcctctt
    41521 gattgcacag gggctggact atcaaacgat tgaacgaggc attgatggga ttgacttgga
    41581 ggagctggaa ggccacttca aaacaggaaa aattaaattt ttctacacca ttcctagatt
    41641 tcactatcct ctgggacatt cctattctga gcaggacaaa cgatctattc ttaacttagc
    41701 tgccaagtat gatgtctata tcgtagagga cgattatctg ggtgatttgg actccaagaa
    41761 gggccaaacc ttccactatc ttgatacaga ggagcgtgtc atttatatca agtccttctc
    41821 gaccagcctt tttcctgccc ttcgtattac agcactcatt cttccaaatg ctatcaaaga
    41881 agcatttgtg gcctacaaaa atatcctaga ctacgacagc aacctcatta tgcaaaaggc
    41941 cctgtcactc tatattgaca gtcaattgtt tgaaaaaaat cgtttggctc gcttgaccaa
    42001 tcatgaatct taccaaaaac aaatcgagga aaggataact aaaacacctt gtccccttcc
    42061 tcattatccc ctacacgatg gtttattgct agacctgaga cagtatccta aaatcgccag
    42121 tctcaaacac agtcaactgg gcttggactt ctttgaagag gcctatttaa gcacctgtcc
    42181 ttatcaattt gccaaggtgt ccctagacaa tctggaaaat gttttaaact atttaaaagc
    42241 agaattggaa tgacatccaa ctctgctttt tcttattctt caacttcagt taaacttgct
    42301 gctttttcaa gatagctttg gtaagttcct tcatccttaa gtttttggat gaccttgtct
    42361 accacttctt tcaaatcatc actatttttt ctaagagcaa cggcattggc gtcgccgtcc
    42421 ttcatcttca agctgacagt tgcgacagct aagccagcgt ttttagcagc ataactaagt
    42481 gcaacaggct catccatatg aacagcatct acttttccag cctgcaattc attgactgct
    42541 tcacccatat tagttaggga agttaattga gcttttggca attgttcctt gaccattgat
    42601 tctggaacag tccctttttg ggctgcaata ttagcacttt ctaggctagt taaatccttg
    42661 tatttttcca catcagcctt acgaaccaag aaactaatct tgttttcata gtatgggatt
    42721 gaaaaatcaa agacttcttt tctctcgtca gtagcactaa ttcctgcaac tgctaggtca
    42781 gccttaccag tttgaagact ggtcaaaaca ttatcaaaac tcatgcttga gatttccaac
    42841 ttaaccccaa gttcatcagc gatagcctga gccatgtcga tgtctgcacc gactacctgg
    42901 ttctttccat caaccaatga ttgaaattca aagggtgcat agtcaggact ggttgccaca
    42961 actaatttcc ccttttgctt aatggcctca acagctgact gagaaccatt agaacctgac
    43021 tggcaagcta ctaagaagaa acttgcaaga aaattacata aaacaaatat ccattttttt
    43081 gatttcataa at
//
LOCUS       DAVLVZ010000012        40424 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000012 DAVLVZ010000000
VERSION     DAVLVZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.012"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            145..6846
                     /locus_tag="VYN77_000590"
     CDS             145..6846
                     /locus_tag="VYN77_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1558093.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVEEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEVPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSDLPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7094..7950)
                     /locus_tag="VYN77_000591"
                     /pseudo
     CDS             complement(7094..7950)
                     /locus_tag="VYN77_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8162..8521)
                     /locus_tag="VYN77_000592"
     CDS             complement(8162..8521)
                     /locus_tag="VYN77_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1558094.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            8613..9569
                     /locus_tag="VYN77_000593"
                     /pseudo
     CDS             8613..9569
                     /locus_tag="VYN77_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9553..10110
                     /locus_tag="VYN77_000594"
     CDS             9553..10110
                     /locus_tag="VYN77_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1558095.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10103..10357
                     /locus_tag="VYN77_000595"
     CDS             10103..10357
                     /locus_tag="VYN77_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558096.1"
                     /translation="MDKQYLREKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10369..12423
                     /locus_tag="VYN77_000596"
     CDS             10369..12423
                     /locus_tag="VYN77_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1558097.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWVALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12602..13462
                     /locus_tag="VYN77_000597"
     CDS             12602..13462
                     /locus_tag="VYN77_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1558098.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13681..14391
                     /gene="ccdA2"
                     /locus_tag="VYN77_000598"
     CDS             13681..14391
                     /gene="ccdA2"
                     /locus_tag="VYN77_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1558099.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14411..14977
                     /locus_tag="VYN77_000599"
     CDS             14411..14977
                     /locus_tag="VYN77_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1558100.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14988..16100
                     /gene="msrB"
                     /locus_tag="VYN77_000600"
     CDS             14988..16100
                     /gene="msrB"
                     /locus_tag="VYN77_000600"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1558101.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKTVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            16368..17105
                     /locus_tag="VYN77_000601"
     CDS             16368..17105
                     /locus_tag="VYN77_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1558102.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            17102..18793
                     /locus_tag="VYN77_000602"
     CDS             17102..18793
                     /locus_tag="VYN77_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1558103.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            18909..19472
                     /locus_tag="VYN77_000603"
     CDS             18909..19472
                     /locus_tag="VYN77_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1558104.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            19494..24962
                     /locus_tag="VYN77_000604"
     CDS             19494..24962
                     /locus_tag="VYN77_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1558105.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAEAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAQPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKSAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQEDNSKEDRGAEETPKQEDEQPAEAPE
                     IKVEEPVESKEETVNQPVEQPKVETPAVEKQTEPTEEPKVEVTTVETREEVIPFETKE
                     QEDDTLKRGTRQVVQKGVEGKKQITETYKTIRGEKTNEAPIVKETVIEQPQDEIIKKG
                     TKGLEKPTLQWANTEKDVLKKSATASYTLTKPAGVEIKSIKLALKDKDGQLVKEVTVA
                     ENNLNATLDKLKYYQGYTLSTTMVYDRGEGEETEKLEDKQIQLDLKKVEIKNIKETSL
                     MNVDAEGNETDKSLLSEKPTDVSQLYLRVTTHDNKVTRLAVSSVEEVVVDGKTLYKVV
                     AKAPDLVQRRADDTLSEEYVHYFEKQLPKVNNVYYNFNELVKDMQANPTGEFKLGADL
                     NAANVKPNGKSYVTKPFKGKLLSNDGGRFTIHNIERPLFANIEGGKIHDINLANVNIN
                     MPWADKVAPIANVIKNNATIENVKVTGNVLGKDWVSGFIDKIDGSGKLINVAFIGNVT
                     SVGTGGNFLTGIVGENWKGYVERAYVDAHIKGKRAKAAGIAYWSQNQGNNFTIGSEGA
                     IKKSVVKGTIDVEKPIEVGGAVGSFTYHGSIEDTVSMMKVKNGEIFYGSKDIDDDPYY
                     TGNHVNRNYVVIGVSEGTSTYRYSNQHNRIKPITQSEADVKIAETAITADKFTITDPI
                     VNKLNALTTRDNEYRTTQDYEATREQAYRNIEKLQPFYNKEWIVNQGNKLVEGSNLLT
                     KEVLSVTGIKSGQFVTDLSDIDKIMIHYSDGAKEELNVTRQESNVQQVREYSITDLDI
                     LYTPNMVEKDRAQLMTDVKSKLSSVELESDGVRQLLVKRDTKKDANANSVGRQNGYIR
                     DLFLEESFSEVKANLDKLVKQILENEDHQLNDNELAERALLKKVEDNKAKIMMGLAYL
                     NQYYAFKYDELSIKDIMMFKPDFYGKTASVIDRLINIGSAENNLKGDRTQDAYRGIIS
                     GATGKGSLHDFLTYNMKLFTNETDINVWFKKAIEKNAYVVEQPSTNPAFANKKYRLYE
                     GINNGQHGRMILPLLNLKNAHLFMISTYNTISFSSFEKYGKDTDEKREKFKSEINKRA
                     KEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHNSPNGWASRHGHIDGKPDYAP
                     IREFFGRINKYHGYKYGYGAYAYIFAAPQPMDAVYFVMTDLISDFGTSAFTHETTHVN
                     DRMAYYGGHWHREGTDLEAFAQGMLQTPSVSNPNGEYGALGLNMAYERQNDGNQWYNT
                     NPNDLTTRAEIDNYMKGFNDTLMLLDYLEGEAVLNKANQDLNNAWFKKVDKQLRGAST
                     KNQYDKVRDLTADEKNITLNSVDDLVDNNFMTKHGPGNNVYDPTGFGTAYVTVPITAG
                     IYGGNTSEGAPGSMSFKHNTFRMWGYYGYEKGFLNYASNMLKNESRQAGHNTLGDDFI
                     IKKVSDNKFSTLEDWKKAYFKEVVDKAKAGFNPVTIDSTTYSSYDDLKNAFAAAVEKD
                     KATLKNGSVKSDNTVALKEKIYKKLLQQTDSFKTSIFR"
     gene            25023..26744
                     /gene="pabB"
                     /locus_tag="VYN77_000605"
     CDS             25023..26744
                     /gene="pabB"
                     /locus_tag="VYN77_000605"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1558106.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKNLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            26745..27362
                     /locus_tag="VYN77_000606"
     CDS             26745..27362
                     /locus_tag="VYN77_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1558107.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            27412..28410
                     /locus_tag="VYN77_000607"
     CDS             27412..28410
                     /locus_tag="VYN77_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1558108.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLTSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTV
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            28537..29496
                     /locus_tag="VYN77_000608"
     CDS             28537..29496
                     /locus_tag="VYN77_000608"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1558109.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTVGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            29593..30432
                     /locus_tag="VYN77_000609"
     CDS             29593..30432
                     /locus_tag="VYN77_000609"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1558110.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRIDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(30480..30650)
                     /locus_tag="VYN77_000610"
     CDS             complement(30480..30650)
                     /locus_tag="VYN77_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1558111.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            30771..31655
                     /gene="miaA"
                     /locus_tag="VYN77_000611"
     CDS             30771..31655
                     /gene="miaA"
                     /locus_tag="VYN77_000611"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1558112.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVLHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHFVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            31648..32886
                     /gene="hflX"
                     /locus_tag="VYN77_000612"
     CDS             31648..32886
                     /gene="hflX"
                     /locus_tag="VYN77_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1558113.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKFQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDS
                     EVITGYISEKNKWRLEEFYD"
     gene            32879..33502
                     /locus_tag="VYN77_000613"
     CDS             32879..33502
                     /locus_tag="VYN77_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1558114.1"
                     /translation="MTDIKALALKYGGYTSLDKVYLVQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     DSDGLGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            33517..34446
                     /locus_tag="VYN77_000614"
     CDS             33517..34446
                     /locus_tag="VYN77_000614"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1558115.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTVRPRKISKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTNASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            34467..35222
                     /locus_tag="VYN77_000615"
     CDS             34467..35222
                     /locus_tag="VYN77_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1558116.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(35276..36184)
                     /locus_tag="VYN77_000616"
     CDS             complement(35276..36184)
                     /locus_tag="VYN77_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1558117.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLSHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            36248..36517
                     /locus_tag="VYN77_000617"
     CDS             36248..36517
                     /locus_tag="VYN77_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1558118.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            36517..36897
                     /locus_tag="VYN77_000618"
     CDS             36517..36897
                     /locus_tag="VYN77_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1558119.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILRARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            36986..37213
                     /locus_tag="VYN77_000619"
     CDS             36986..37213
                     /locus_tag="VYN77_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558120.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLEYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(37253..37978)
                     /locus_tag="VYN77_000620"
     CDS             complement(37253..37978)
                     /locus_tag="VYN77_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV1558121.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            38140..39981
                     /gene="typA"
                     /locus_tag="VYN77_000621"
     CDS             38140..39981
                     /gene="typA"
                     /locus_tag="VYN77_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1558122.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            40001..40255
                     /locus_tag="VYN77_000622"
     CDS             40001..40255
                     /locus_tag="VYN77_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1558123.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT        12413 a         7518 c         9023 g        11470 t
ORIGIN      
        1 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgctgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caagcttcaa gtcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggta ctttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgcagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatacgat atcaccaaat accttcataa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaat
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttat ggatcagaaa catcttcagc tacccgtaca
     2101 cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt ctgactatgg gaatgatcgt gttggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctacctcta ccaaagccaa
     2401 tgggtttctg ttaaaaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt gttcttgaat ggaaaatctc ttggtcttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccgggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagtcg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc acccttactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaag aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtgc caaaagtaca gaccattatt ggagaggcac ctgaaatgcc taccactgtt
     3181 ccgtttgtat acagtgatgg tagccgtgca gaacgtcctg taacctggtc ttcagtagat
     3241 gtgagcaagc ctggtattgt aacggtgaaa ggtatggctg acggacgaga agtagaagct
     3301 cgtgtagaag tgattgctct taaatcagag ctaccagttg tgaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcctatgttt tgactgatgg aagtgttgaa
     3421 gagtatgaag tggacaagtg ggagattgcc gaagaagata aagctaagtt agcaattcca
     3481 ggttctcgta ttcaagcgac cggttattta gaaggtcaac caattcatgc aacccttgtg
     3541 gtagaagaag gcaatcctgc ggcacctgca gtaccaactg taacggttgg tggtgaagct
     3601 gtcacaggtc ttactagtca aaaaccaatg caataccgca ctcttgctta tggagctaag
     3661 ttgccagaag tcacagcaag tgctaaaaat gcagctgtta cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggccc tcttcaaacc
     3781 tatgcaattc aattccttga agaagtgcca aaaattgctc acttgagctt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgat aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctgaac
     4021 gctgaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagtgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtcca
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaagg tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagactt gccacttgcc tttgcttcag actcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgtcc tattcggaga ttcaggtatt
     4741 ttgagcaaac gttcagttga taacttaagc gttggcttcc atgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtga gagcagacaa caaactagga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa gcaaggacaa
     5101 acaagaatcc aagttgacgg taaagactta gcaaacttca accctgatct gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagat
     5461 ttgtcagttg aatgggaaga agttccagca gaaaatctga caaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcg
     5821 caagccaaac ttcacttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagcagat aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac caagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaataat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc acttgactaa ggaaaaacca gtttctgaga agacagttgc tgctgtacaa
     6421 gaagatcttc caaaactcga atttgttgaa aaagatttgg cctacaagac agttgagaaa
     6481 aaagattcaa cactgtatct aggtgaaact cgtgtagaac aagaaggaaa aactggtaaa
     6541 gaacgtatct ttacagcgat taatcctgat ggaagtaagg aagaaaaact ccgtgaagtg
     6601 gtagaagctc cgacagaccg catcgtcttg gttggaacca aaccagtagc tcaagaagct
     6661 aaaaaaccac aagtgtcaga aaaagcagat acaaaaccaa ttgattcaag tgaagctagt
     6721 caaactaata aagcccagtt accaaataca ggtagtgcgg caagccaagc agcagtagca
     6781 gcaggtttag ctcttctagg tttgagtgca ggattagtag ttactaaagg taaaaaagaa
     6841 gactagaaat tttgatacct tctttatcat taaagaccaa agaaaaactc ttgtcctaaa
     6901 tagaagataa agagtctgga acaaaatagt tctcaaccac aaaaaagcta gatatttcca
     6961 attgcggaac tctagctttt taattttgag tcgttctctt attgtatagt atattgaaac
     7021 tagaatagta cacttctact tctaaaacat tgttagaaat caatttgact gtccttatcg
     7081 atttgtcctg ttcttatttc atttcactat gttttaggag gttattggcc ataagtacca
     7141 atcccatgtc aattctcact tgacgcttcc ctctcagatt acatctcttg taacccaaac
     7201 aagcctttat ctgcccaaag acaggttcca catcaatctt gcgttgagcg aaaatctgtc
     7261 taccttgggg agataaaagc gcctgacatt ctttagcttt caagttttga tagcgttcgt
     7321 tcatatacag tcccttttga ggggctgatt caggttcatc agcgtagtaa accttgattt
     7381 cctgttgaaa gtctgtctgt gttttctgat gtttggtatg gtgaaaacga taataccagc
     7441 catcaggatg tgtgtagcta tcctccttgt cattatagtg ccaattcgct aagtttttag
     7501 ctgactcttt ataccctctc ttttgtttct tatcaaacat ggcatattta atcagaaggg
     7561 gagtgatttc ttcttgaaaa tagacctgta tctgttcttg aagtttggtg gagaatttct
     7621 ctgtcgtttt cttccacaca aaactatact tgttggcatt ggcttcaatc ttagttccgt
     7681 caataaacaa acaatctaag gtcactaact cttccatttt tcaatttttc gaccagagaa
     7741 aatccctttt gaataggcaa atagaagagt agatacaagc attttagggt gataagacgg
     7801 gcgatcaaag gcatgatagg aggtgtagaa gtgacgttcc tccaaggtat tcaccacttt
     7861 ttcaatagta aagacgagat ggtcttgtgg taagaaggaa ctgatttcta gtggtaaagt
     7921 tgtttgattt gtgttatagt gaatatgcat gggatagcct ttttaaatgg tttattaagc
     7981 aattctattt taccaagact atcgtaacca tgcaaaaaaa gcaacggcaa atccgttgct
     8041 ttttttggag ataagagact attgtcccag actcttttct ttgtataaaa ttgtatagtg
     8101 ttattgtcta tactattttg tagagaaagc aaaaatccct agaaaatgct tagggattcg
     8161 actatttcat taagaacaat tcaacaatca tttctatata catgataagg gttaataaga
     8221 atcctgcacg ccagaagaat ttgataaatt taggatagta aaagctgcgt tttttcaaaa
     8281 ggaagaaaaa gacgagaata atggctagga tcgagagggc tagccccagt ctagggagga
     8341 aattatgggt aaaggtttta gctgtaatca agtaatactc aaataccaag actggaaaag
     8401 ccaaatccgc aaagtttcgt cctagttttt ttaatctaaa aagtttggtt aggataatgc
     8461 agacgactaa ggtcagtatc aataataaaa tagatgctaa tttcattaaa atcataccca
     8521 tattgtatca taaaaaaagg ctaatggaaa cgagaaacag aggaatttat aggaaagtca
     8581 ggcttttgag attgtgggtg atttttgtta taatgaaagt catgaaatct tataatacct
     8641 tgaatgatta ttatcgaaaa ctctttggag aaaagacttt taaagtccct attgatgcgg
     8701 gatttgactg tcccaatcgt gatggaactg tggctcatgg aggcagtact ttttgtacgg
     8761 tttcaggttc tggagatgct attgtagccc cagatgcgcc tatccgtgag caattttata
     8821 aggaaattga ctttatgcac cgtaagtggc cggatgttca gaagtatctg gtttattttc
     8881 aaaattttac caacacccat gaaaaggtgg aagttattcg agagcgttat gaacaggcta
     8941 tcaatgagcc aggtgtggta ggtatcaata ttggaacgcg cccagactgt ttaccagacg
     9001 aaaccatcga atatttggct gagttatcgg agtgcatgca tgtgacggtt gaattgggct
     9061 tgcagaccac ttatgaagca acctctgacc tgattaaccg tgtgcattct tatgaattgt
     9121 aggtggaaac ggtcaagcgt ttgagaaagt atcccaagat tgagattgtt tcccatttga
     9181 tcaatggttt gcctggtgaa acccatgaga tgatggttga aaatgtccgc cgttgtgtca
     9241 cggataacga tattcaaggg attaaactgc atttgctcca tcttatgaca aatactcgta
     9301 tgcaacgaga ttaccatgag ggacgtttac aattgatgag tcaggacgaa tatgtcaggg
     9361 tcatctgtga ccaactggaa attattccca aacatatcgt catccatcga attacaggag
     9421 atgcgcctag ggatatgctg attggtccta tgtggagcct caaaaaatgg gaagtcctca
     9481 acagcattga gatggagatg cgacgtcgtg gaagtgttca aggatgcaag gctgtaaaac
     9541 aggagtttga aaatgaaaag accacttgag atggcacatg attttttggc tgaggtcgtg
     9601 acaaaagagg atgtcgtagt ggatgcgact atgggaaatg gtcatgacac gcttttttta
     9661 gccaagctag ccaagcaagt ctatgccttt gatattcaga agcaagcctt ggaaaagacc
     9721 caagagcgtt tgcatcaggc tggcttgaca aatgcccagt taatcttgca aggccatgag
     9781 acactggacc agtttgtgac aaaagctaag gcagggattt ttaatctggg ctatttgccg
     9841 tcagctgata agtctgtcat cacccgaccg cagacaacga ttgaggcatt agaaaagcta
     9901 tgtggcttac ttgtcaaagg tggacgaatt gctattatga tttactatgg tcatgaagga
     9961 ggcgacctcg agagagatgc tgtcttggat tttgtaatcc agttgaacca acaagagtac
    10021 acagctgcca tttaccgaac tttaaatcaa gtcaacaacc cgccgttttt agtgatgatt
    10081 gaaaaattag agagatatag acatggataa acaataccta cgtgaaaagc tggatgccat
    10141 gcgccagaat tttgttgaat caacccacca cgaacgagcg gtgggcgtgc tagaccaagc
    10201 gcatatgagc aaaaaaatgc ttaaaatcaa gaaaaaatta gttgctcttg aaatggaacg
    10261 gtgccagaga aaaattgagc acaaagactg ttccaagatt gaccaaaaaa tcaaagagca
    10321 gaaggagata tttgaatcct gttgtaaaaa agattaagga ggacgtgcgt ggaattactg
    10381 atttacctca tcctattttt actggtcttg attgtctcaa gtacaaccaa taagctcctg
    10441 ccctttttgc ctctcccttt ggtgcagatt cttttgggaa ttgtgattgg tctcttttta
    10501 cccaatactg actttcatct taatacggag ttgtttttgg ccctggttat cggacccttg
    10561 cttttccgag aggctgaaga agcagatgtt acggctattt taaaacactg gcgaatcatt
    10621 gtttatctca tatttccagt gatttttatc tcgaccctga gtttgggtgg cttggcccat
    10681 cttctttggt tcagccttcc cttggcagct tgcttggctg ttggggcagc ccttggtcct
    10741 acggacttgg tggcctttgc ctctctttcg gagcgtttta gctttcctaa gcgcgtgtcc
    10801 aatattctta agggcgaagg actcttgaat gatgcttctg gtttggtggc ttttcaggta
    10861 gctttgacag cttggacaac tggagctttt tctctggggc aagctagcag ttcgctcatc
    10921 ttttcaatcc taggcggttt tttaattgga tttttaacag ccatgaccaa ccgcttcctc
    10981 cataccttct tgctaagtgt gcgcgcaacg gatattgcca gtgaactttt attagaattg
    11041 agtttgcctc tagtgacctt ctttctggca gaagaagtcc atgtttcagg gattattgcc
    11101 gtcgtagttg ctggaatttt aaaggcaagt cgcttcaaga aaatcacgct cctcgaagcc
    11161 caagtggata cggtgaccga gacggtctgg catacagtga cctttatgct caacggttct
    11221 gtctttgtga ttttagggat ggagttggaa atgatagcag aacctatctt gaccaatcca
    11281 atctataatc ctctactttt attgctatct ctcatcgccc ttacctttgt cctctttgtc
    11341 attcgtttta ttatgatcta tggctattat gcctatagaa cccgacgcct caagaaaaag
    11401 ctaaataagt atatgaagga catgtttctc ttgacctttt caggtgttaa gggaacggtg
    11461 tcgattgcta cgattctctt gataccaagt aatctagaac aggagtatcc tctcttgctt
    11521 ttccttgttg caggtgtgac gcttgtcagc tttttaacag gtctcttggt cttgcctcat
    11581 ctttctgatg aagaggaaga aagcaaggat tatctcatgc atatcgccat tttgaatgaa
    11641 gtaacgctag agttggaaaa agagttggaa gacaccagaa ataaacttcc cctctatgcg
    11701 gctattgaca attatcatgg acgtattgaa aatctcattt taagccaaga aaaccaggat
    11761 gatcaagaag actgggttgc tttgaaactc ttaattctta gtattgaaag tgatggtttg
    11821 gaacaggcct atgaagaggg gaacattagc aatcgtgctt accgagttta ccaacgttat
    11881 ctgaaaaata tagaacaagg aatcaatcgt aaacttgcct caagactgac ctattatttt
    11941 cttgtttcct tgaggatttt acgttttctt cttcatgaag tttttactct tggaaagacc
    12001 ttccgtagct ggaaggacaa ggagcaaagc cgtctccgtg ctcttgatta tgaccaaatt
    12061 gcagagctct atcttgccaa tacagagatg attattgaaa gtttggaaaa cctgaaggga
    12121 gtctacagac gctctttgat tagttttatg caggagtctc gtcttcgaga aacagctatt
    12181 atcagcagtg gtgcctttgt cgaacgggtt atcaatcgtg tcaaacccaa caatatcgat
    12241 gaaatgctga gaggctatta tctggagcgc aagttgattt tcgaatacga agaaaaacga
    12301 ttgattacga ctaagtatgc caagaaatta cgacaaaatg taaataactt agagaactat
    12361 tccttgaagg aagctgccaa taccctgccg tatgatatgg tggaattggt aaggagaaat
    12421 tagttaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttgg attatatatg
    12481 tgactgactt cgtcagtttc atctacaacc tcaaagcagg gctttgagca acctgcggct
    12541 agcttcctag tttgctcttt gattttcatt gagtataaga ttgtaagtga aggagtgtga
    12601 catgaaaaaa tggtggaaag agctgataga caagccttta ttaaaagctt ttttgcatta
    12661 ttatcaagca tcagatagtg agttgaccag tgtcgcagta gcctactatt ggttgatttc
    12721 gattttcccc ctgcttttgg tggtggtcaa tatcctccct tattttcaga ttcctgtggg
    12781 agaatttctg ggctttatga aagacgtcct gcccccaagc ctctatgaag gtgtggaaaa
    12841 aatagccaga gaagtcttga cgcaaccttc aacaggttta ttgagttttt ctgttttatc
    12901 ggcgttatgg agtttttcaa aatctatgaa tttcttgcaa aaagctttta acaaggctta
    12961 tggcgtcgaa aagagtcgtg gacttatttc ccatcagatg ctgagtctcc tagtcagttt
    13021 tggcttgcag ctcctctttg cgtttgcctt gtttttgatc ttatttggtc agatgatttt
    13081 ggctttactt gctcattatt ggacaaaaga tggcattatc tatcaggctt tacaagggtt
    13141 ggcaggtcct ctgatttacg ccctcctctt tgctatcttg gtcatgcttt attattttct
    13201 tcccaatcta tctaacagga aaattagcta taccctacca ggcagtgcct ttgttctctt
    13261 agtgatttta ggtttactaa ctctgttctc tagttatctc aattactatg tccatcattt
    13321 ggtggatgtc cgaattttag gttccgttct tcttgttgtt atgatgtttt ggtttatctt
    13381 gattgctaaa attgttatcc taggcgcggt tatcaatgcc agtatgcaga gtttgaaaga
    13441 tccggtcttt aaaaaagact aactatttat ccattataaa agcgcatagt atcaagactt
    13501 tatagaacct tgatactatg cgcttttgcg cttttgtgct ttgacagttt tttgtatagt
    13561 ttttgtccaa gatttctaaa tcatctgatt aaattcctat tttcccctat cattgtccct
    13621 gttttttcga aaatggaagg agtataataa atctatcaat aaaatgatag gaggaacttt
    13681 atgggtcata ttttcttttt tctaagtgtc tttttggcag ggattctatc cttcttttct
    13741 ccttgtatct tacctttgtt accggtctat acaggagtgt tactagatga taaggatggt
    13801 gctcaggctt ctagcggcaa attttcaatc tcagttacta gtttattacg aactctggcc
    13861 tttatagcag gaatttcctt tatatttatt ttgttgggct atggagctgg ttttttaggc
    13921 gatttgcttt atgcttcttg gttccaatat cttactgggg caattattat ccttcttggt
    13981 ttgcaccaaa tggagattct acactttaag gggctttata aggaaaagag gctacaactg
    14041 caaggacagg ggcaaaatgg taagggctat agtcaggcat ttttattggg cttgaccttt
    14101 agttttgctt ggacgccttg cgtggggccg gttctggggt ctgttttggc cttggcggct
    14161 tcaggtggtt caggagcttg gcagggagct ggtctcatgt tggtgtatac gctgggcttg
    14221 gcgctaccat tcttgcttct agctctgacc tctagttatg ttttgaaaca tttccgaaaa
    14281 cttcatccct atctcggaat cctcaaaaaa gtgggtggtt ttctcattat tgtgatggga
    14341 ctcttggttc tgtttggaaa tgcttcaatt ttaagtcaat tatttgaata aaatggaaag
    14401 gaatatcaat atgaaaaaat ggcaaacatg tgttcttgga gcaggttcgc tcctttgttt
    14461 gacggcttgt tcaggcaagt ccgtgactag tgaacaccaa acgaaagatg aaatgaagac
    14521 ggagcagaca gctagtaaaa caagcgcagc taaagggaaa gaggtggctg attttgaatt
    14581 gatgggagta gatggcaaga cctaccgttt atctgattac aagggcaaga aagtctatct
    14641 caaattctgg gcttcttggt gttccatctg tctggctagt cttccagata cggatgagat
    14701 tgctaaagaa gctggtgatg actatgtggt cttgacagta gtgtcaccag gacataaggg
    14761 agagcaatct gaagcggact ttaagaattg gtataaggga ttggattata aaaatctccc
    14821 agtcctagtt gacccatcag gcaaactttt ggaaacttat ggtgtccgtt cttacccaac
    14881 ccaagccttt atagacaaag aaggcaagct ggtcaaaaca catccaggat tcatggaaaa
    14941 agatgcaatt ttgcaaactt tgaaggaatt agcttaggag gtgtcttatg aatgataagt
    15001 taaaaatctt cttgttgcta ggagtatttt ttctagccat aaccggtttc tatgttctat
    15061 tgatacgaaa tgcagggcag acagatgcct cgcaaattga aaaggcggca gttagccaag
    15121 gaggaaaaac agtgaaaaaa acagaaatta gtaaagacgc agacttgcac gaaatttatc
    15181 tagctggagg ttgtttctgg ggagtggagg aatatttctc acgcgttccc ggggtgacgg
    15241 atgccgtttc aggctatgca aatggtagag gagaaacaac caagtacgaa ttgattaacc
    15301 aaacaggtca tgcagaaacc gtccatgtca cctatgatgc caagcaaatt tctctcaagg
    15361 aaatcctgct tcactatttc cgcattatca atccaaccag caaaaataaa caaggaaatg
    15421 atgtggggac ccagtaccgt actggtgttt attacacaga tgacaaggat ttggaagtga
    15481 ttaaccaagt ctttgatgag gtggctaaga aatacgatca acctctagca gttgaaaagg
    15541 aaaacttgaa gaattttgtg gtggctgagg attaccatca agactatctc aagaaaaatc
    15601 caaatggcta ctgccatatc aatgttaatc aggcggccta tcctgtcatt gatgccagca
    15661 aatatccaaa accaagtgat gaggaattga aaaagaccct gtcacctgag gagtatgcag
    15721 ttacccagga aaatcaaaca gaacgagctt tctcaaaccg ttactgggat aaatttgaat
    15781 ccggtatcta tgtggatata gcaactgggg aacctctctt ttcatcaaaa gacaaatttg
    15841 agtctggttg tggctggcct agttttaccc aacccatcag tccagatgtt gtcacctaca
    15901 aggaagataa gtcctacaat atgacgcgta tggaagtgcg gagccgagta ggagattctc
    15961 accttgggca tgtctttacg gatggtccac aggacaaggg cggcttacgt tactgtatca
    16021 atagcctctc tatccgcttt attcccaaag accaaatgga agaaaaaggc tacgcttatt
    16081 tactagatta tgttgattaa tactcttcga aaatctcttc aaaccacgtc agcgtcgcct
    16141 taccgtatgt agggttactg actccgtcag ttttatctac aacctcaaag ctatactttg
    16201 agctgacttc gtcagtttta tctacaacct caaagcggta ctttgagaaa cctgtggcta
    16261 tcttcctagt ttactttttg atttttattg agtataagag gtctttccta agcagttaga
    16321 ggagaatttt gctatactga tactagtaag tggtaaagga gcagagcatg acctacacaa
    16381 tcttaatcgt agaagatgaa tatctggtaa gacaaggttt gactaaactg gtcaatgtag
    16441 cagcctacga tatggaaatc atcggtcagg ctgaaaatgg aaggcaggct tgggaattga
    16501 tccaaaagca ggttccagat atcattttaa ccgatatcaa catgcctcat ctaaatggca
    16561 tccagttggc cagtctggta cgagaaacct atcctcaggt tcatttggtc tttttaacag
    16621 gttacgatga ttttgattat gccttgtctg ctgtcaaact aggtgtggac gactacctgc
    16681 tcaaaccctt ttctcgtcag gatattgagg aaatgttggg gaaaatcaaa caaaaactag
    16741 acaaggaaga gaaagaagag cagttacaag atttattgac taataggttt gaaggaaaca
    16801 tggcccagaa aatccagtct catctggctg atagccaatt tagtttaaag tctttagcca
    16861 gtgacttagg ttttagtccg acctatctga gttccttgat taagaaagag ttgggcttgc
    16921 cttttcagga ttatctggtg agagaacgtg ttaaacaagc caagctcttg cttttaacta
    16981 cagatctgaa gatttatgag atcgcagaga aggttggttt tgaagatatg aactatttta
    17041 cccaacgttt taagcagatt gcaggtgtga cacctcgtca gtttaagaag ggagaagacc
    17101 gatgaagcgt tcttctcttt tagttagaat ggttatttcc atctttctgg tctttctcat
    17161 tctcctagct ctggttggaa ctttctacta tcaatcaagt tcttcagcca ttgaggccac
    17221 cattgagggc aacagccaaa cgaccatcag ccagactagc cactttattc agtcttatat
    17281 caaaaaacta gaaaccacct cgactggttt gacccagcag acggatgttc tggcctatgc
    17341 tgagaatccc agtcaagaca aggtcgaggg aatccgagat ttgtttttga ccatcttgaa
    17401 gtcagataag gacttgaaaa ctgttgtgct ggtgaccaaa tctggtcagg tcatttctac
    17461 agatgacagt gtgcagatga aaacttcctc tgatatgatg gctgaggatt ggtaccaaaa
    17521 ggccattcat cagggagcta tgcctgtttt gactccagct cgtaaatcag atagtcagtg
    17581 ggtcatttct gtcactcaag aacttgttga tgcaaaggga gccaatcttg gtgtgcttcg
    17641 tttggatatt tcttatgaaa ctctggaagc ctatctcaat caactccagt tggggcagca
    17701 gggctttgcc ttcattatca atgaaaacca tgaatttgtc taccatcctc aacacacagt
    17761 ttatagttcg tctagcaaaa tggaggctat gaaaccctac atcgagacag gtcagggtta
    17821 tactcctggt cacaaatcct acgtcagtca agagaagatt gcaggaactg attggacggt
    17881 gcttggcgtg tcatcattgg aaaagttaga ccaggttcgg agtcagctct tgtggacctt
    17941 gcttggggcc agtgtcacat ctcttcttgt ctgtctctgc ttagtgtggt tcagtcttaa
    18001 acgctggatt gctcctttga aggatttgag agaaaccatg ttggaaattg cttctggtgc
    18061 tcaaaatctt cgtgccaagg aagttggtgc ctatgaactg agagaagtaa ctcgccaatt
    18121 taatgctatg ttggatcaga ttgatcagtt gatggtagct attcgtagcc aggaagaaac
    18181 gacccgtcag taccaacttc aagccctttc gagccagatt aatccacatt tcctctataa
    18241 cactttggac accatcatct ggatggctga atttcatgat agtcagcgag tggtgcaggt
    18301 gaccaagtcc ttggcaacct atttccgctt ggcgctcaat caaggcaagg acttgatttg
    18361 tctctctgac gaaatcaatc atgtccgcca gtatctcttt atccagaaac aacgctatgg
    18421 agataagctg gaatatgaaa ttaatgaaaa tgttgccttt gataatttag tcttacccaa
    18481 gctggtccta caaccccttg tagaaaatgc tctttaccat ggcattaagg aaaaggaagg
    18541 tcagggccat attaaacttt ctgtccagaa acaggattcg ggattggtca tccgtattga
    18601 ggatgatggc gttggcttcc aagatgctgg tgatagtagt caaagtcaac tcaaacgtgg
    18661 gggagttggt cttcaaaatg tcgatcaacg gctcaaactt cattttggag ccaattacca
    18721 gatgaagatt gattctagac cccaaaaagg gacgaaagtt gaaatatata taaatagaat
    18781 agaaactagc taactcccag tcaattctgg gagttttatg cgtaattggg caaattttct
    18841 aggttgtcta tcttgctaaa aacaagaaaa aacggtatga tagaagagtg acaacagagg
    18901 taacagagat gaacgagaca cagattcaaa gagaaacacg tcaggtagtg gaagatgttt
    18961 tagaaaagac caatttgaag cagggggccc tttttgtttt aggtctatct tctagtgagg
    19021 tgctaggtgg tcagattggg aaagaatcca gtcaagagat tggtgagctg atagtggaga
    19081 ctatcttggg tattctaggt agtagaggta tccatttagc agttcaaggt tgtgagcatg
    19141 tcaatcgggc tctcgttgtt gaacgtcagg tggcagagca gtttgatctg gaaattgtca
    19201 gtgtccatcc tactcttcat gcaggaggtt cggggcagtt ggcagccttt aagtttatgc
    19261 aggatccagt tgaggttgaa tttatcaagg ctcatgctgg attggatatc ggagacactg
    19321 caattggcat gcatgtcaag catgttcagg ttccgattcg ccctattttg agagagattg
    19381 gtcatgccca tgtaacggca ctggctagtc gtccaaaatt aatcggaggt gcgcgtgcgc
    19441 actatccgca agacgctatt agaaagtctt gagaatgaat aaaggagata aaagtgttta
    19501 aaaaagaccg tttttcaatt cgtaagatta agggagttgt aggctctgta tttcttggaa
    19561 gccttttgat ggctccttct gtagtggacg cagccaccta tcactatgta aataaagaga
    19621 ttatttcaca agaagctaaa gatttaattc agacaggaaa gcctgacagg aatgaagttg
    19681 tatatggttt ggtgtatcaa aaagatcagt tgcctcaaac agggacagaa gcatctgttt
    19741 tgacagcttt tggtttgctg actgttggga gcttgctttt aatctacaag agaaagaaaa
    19801 ttgctagcgt ctttctagtt ggagctatgg gattggtagt tcttcctagt gcagaggctg
    19861 tagacccagt tgcgacccta gcgctggcta gtcgagaggg tgttgttgaa atggatggct
    19921 atcgctatgt tggttatcta tcaggtgaca tcctcaaaac gcttggcttg gacactgttt
    19981 tagaagaaac ctcagcacaa cctggagagg tgactgtagt cgaagttgag actcctcaat
    20041 caacaacaaa tcaggagcaa gctaggacag aaaaccaagt agtagagaca gaggaagctc
    20101 caaaagaaga agcacctaaa acagaagaaa gtccaaagga agaaccaaaa tcggagataa
    20161 aacctactga cgacaccctt cctaaagtag aagaggggaa agaagattca gcagaaccag
    20221 ctccagttga agaagtaggt ggagaagttg agtcaaaacc agaggaaaaa gtagcagtta
    20281 agccagaaag tcaaccatca gacaaatcag ctgaggaatc aaaagttgaa ccaccagtag
    20341 aacaagcaaa agtcccagaa caacccgtgc aacctacaca agctgagcaa ccaagtacac
    20401 caaaagaatc atcacaagaa gacaattcta aagaagatag gggagcggaa gagacaccga
    20461 aacaagaaga tgaacagcca gcagaagccc cagaaatcaa ggttgaagaa ccagtagaat
    20521 caaaagagga gactgttaat caacctgttg aacaaccaaa agtggaaacg cctgctgtag
    20581 aaaaacaaac ggaaccaaca gaggaaccaa aagttgaagt aacaacagtt gaaacaagag
    20641 aagaagtcat tccttttgaa acaaaagaac aagaagatga cactttaaaa cgcggaacaa
    20701 gacaagtagt tcaaaaaggt gtagaaggta agaaacaaat tactgaaact tataaaacaa
    20761 ttcgtggaga aaaaacaaat gaagctccaa tagttaagga aacagtaatt gaacaacctc
    20821 aagatgagat tatcaaaaaa ggtacaaaag ggttagaaaa accaacatta caatgggcta
    20881 atactgaaaa agatgtcttg aagaagagtg ctacagctag ttatacattg actaaaccag
    20941 ctggtgtaga aattaaatca attaaattag cgctaaaaga taaagatgga caacttgtta
    21001 aagaagtaac ggtagctgaa aataatttaa atgctacttt agataaattg aaatattatc
    21061 aaggttacac cctatctact acaatggttt atgatcgtgg agaaggtgaa gaaactgaga
    21121 aattagaaga taaacaaatt caattagacc ttaaaaaagt tgaaatcaaa aacattaaag
    21181 aaacaagctt aatgaatgtt gatgctgagg gtaacgaaac tgataagagc ttattatctg
    21241 aaaaaccgac agatgtttcc caattgtatt tacgtgtgac aactcatgac aacaaagtga
    21301 caagacttgc ggtaagttca gttgaagaag ttgttgttga tggaaaaaca ttgtacaaag
    21361 ttgtagccaa agctccagac ttagttcaac gtagagctga tgatacactg agtgaagaat
    21421 atgttcatta ttttgaaaaa caattaccaa aagtaaataa tgtatactac aacttcaatg
    21481 aacttgtaaa agatatgcaa gctaatccaa cgggtgagtt taaacttggt gcagacttga
    21541 atgcagcaaa tgtaaaacct aacgggaaat cttatgtaac gaaaccattt aaagggaaat
    21601 tactaagtaa tgacggcggt agatttacaa tccataatat agaacgtcca ttattcgcta
    21661 atattgaagg tggtaaaatc cacgatatta atcttgctaa tgtaaatatt aatatgcctt
    21721 gggctgataa agttgctcct attgcaaatg taatcaaaaa taatgcaacc attgaaaatg
    21781 tcaaagttac tggtaatgtc ctgggtaaag actgggtatc tggatttata gacaaaattg
    21841 acggcagtgg taaattaatt aatgttgcat ttatcggtaa tgttacatct gttggtactg
    21901 gaggcaactt cttaacaggt attgtgggag aaaactggaa aggatacgtt gaaagagcat
    21961 atgttgatgc tcatattaaa ggtaagcgag ctaaagctgc gggtatagcc tactggtcac
    22021 aaaaccaagg gaataacttt actattggat ctgagggggc tattaaaaaa tctgttgtta
    22081 aaggtacaat agacgttgaa aaaccaattg aagttggtgg cgctgttgga agttttactt
    22141 atcatggttc tattgaagat actgtttcta tgatgaaagt taagaacggt gaaattttct
    22201 atggttctaa agatattgat gatgatccat attacacagg taaccatgtt aatcgaaact
    22261 acgttgttat aggtgttagc gaaggtactt ctacatatag atattctaat cagcataatc
    22321 gtattaaacc tataacacaa tcagaagctg atgttaaaat tgcagagaca gctatcacag
    22381 cagataaatt tacaattacc gatccaattg taaataaact taacgctctc acaacacgtg
    22441 ataatgagta tagaacaaca caagattacg aggcgactcg cgaacaagct tatcgcaata
    22501 ttgaaaaact tcaaccgttc tataacaaag aatggattgt caaccaaggt aacaaacttg
    22561 tagaaggttc aaacttgttg acaaaagaag tattatctgt tacaggtata aagagcggtc
    22621 aattcgtaac tgatttgtca gatattgata aaattatgat tcactattct gatggagcaa
    22681 aggaagaatt gaatgttact cgtcaagaat caaacgttca acaagtgcgt gagtacagta
    22741 ttacagattt agatatactc tatacaccaa atatggttga gaaagatcgc gctcagttga
    22801 tgactgatgt aaagtctaag ctatctagtg tagaattaga gtctgatgga gtgcgtcaac
    22861 ttctcgttaa gagagatact aagaaagatg cgaatgcaaa tagtgtaggt cgtcaaaatg
    22921 gatacattag agatttattc cttgaagaaa gtttctctga agttaaagca aatcttgaca
    22981 aattggtaaa acagatactt gaaaatgaag atcatcaact caacgataac gaattagctg
    23041 aacgtgcttt actgaagaaa gttgaagata ataaagctaa aatcatgatg ggactggcat
    23101 acttaaatca atattatgct tttaaatacg atgagttatc aattaaagat attatgatgt
    23161 ttaaacctga tttttatggt aagacagcaa gtgttattga tagattgatt aatattggtt
    23221 ctgcagaaaa taatctaaaa ggagatagaa cgcaagatgc ttatcgtggt ataatttcag
    23281 gggcaacagg taaaggtagt ttacatgact tcttgaccta caatatgaaa ttattcacaa
    23341 atgaaacaga cataaatgtt tggtttaaga aagctattga aaagaatgct tatgtagttg
    23401 aacaaccatc aacgaaccct gcatttgcca acaaaaaata tcgtctatac gaaggaatta
    23461 ataatgggca acatgggcgc atgattttac ctcttcttaa cttgaaaaat gcacatctat
    23521 ttatgatttc aacatacaac actatctcgt ttagttcgtt tgaaaaatat ggcaaagaca
    23581 cagatgaaaa acgtgaaaag ttcaagagtg agatcaataa acgtgctaaa gagcaggtta
    23641 attacttaga tttttggtca cgtcttgcta ctgataatgt ccgtgataaa cttcttaaga
    23701 gtcaaaatgt agttccaact cctgtatggg ataatcataa tagcccgaac ggatgggcta
    23761 gtagacatgg tcatatagat gggaaaccag attatgctcc aattcgtgag ttctttgggc
    23821 gaatcaataa gtatcatggt tacaaatatg gatatggcgc atatgcatat atttttgcag
    23881 cacctcaacc gatggatgca gtttatttcg ttatgacaga tttaattagt gactttggaa
    23941 catctgcatt tacacatgaa actactcacg ttaatgatcg tatggcttac tatggtggtc
    24001 attggcatcg tgaaggtacg gatcttgaag catttgctca ggggatgtta caaacgccat
    24061 cggtatcaaa tccaaacgga gaatatggcg cattaggtct aaatatggcg tatgaacgtc
    24121 aaaatgatgg aaatcaatgg tataacacaa atccaaacga ccttacaact cgtgcagaaa
    24181 ttgataacta catgaaaggt ttcaacgaca cattaatgct tcttgattac cttgaaggag
    24241 aagctgtcct caataaagca aatcaagatt taaacaacgc ttggttcaaa aaagtggata
    24301 agcaacttcg tggagctagt acgaagaacc agtatgataa agttcgtgat ttaacggcag
    24361 atgagaaaaa tatcactcta aattcagtag atgatttagt tgataataat tttatgacga
    24421 aacacggacc tggaaacaat gtatatgatc caactgggtt cggaacggcc tacgtgactg
    24481 tacctattac tgcaggtatt tatggaggta acacaagtga aggtgctcct ggttctatgt
    24541 cattcaaaca taacacattc agaatgtggg ggtactacgg atatgaaaaa ggattcttaa
    24601 actatgcttc taacatgctg aaaaatgagt ctagacaggc tggtcacaat actctaggtg
    24661 atgatttcat cattaagaaa gtttctgata ataaattcag tacattggaa gattggaaga
    24721 aagcatactt caaagaagtt gtagataaag ctaaagcagg atttaaccct gttacaatcg
    24781 acagtactac atatagtagt tacgatgact tgaagaacgc atttgctgca gcagttgaaa
    24841 aagataaagc tactcttaaa aatggatcag ttaaatctga caatactgtt gcacttaaag
    24901 agaaaatata caaaaaactt cttcaacaaa cagatagctt taaaacttct atctttagat
    24961 aatcgaaatc tctcatctgc tttgcggatg agggttttct tattttatgc tatacttaag
    25021 gtatgcatag aaaaacagtg attgatttta ggactttagg ggagagatat acctttaccc
    25081 agcctattaa agagttgaaa acgagaaatg tagaagaagt ggcagatttg ctggcacaag
    25141 tggaaagcta ccaagagcaa ggttattatg tggtggggta tgtcagctac gaggctgcac
    25201 ctgcttttga ggaaaaatta gcagttcaca aggatcccct actgggagag tacctgcttt
    25261 attttactgt tcatgatagc gtagaaactt cccctattcc tctgacttat gaggatattg
    25321 atttgccctc aaattggcag gaagtaacgt ctgcagcaga ctatgaaaag gccattgccc
    25381 agatacacca tcacttgagg cagggagaca cctatcaggt caactacacc gtccaactca
    25441 agcaaaagtt aagtgccaat ccttttgcca tctacaatcg tatggtggta gagcaggagg
    25501 cgggctacaa tgcctatgtg gaacatgacg agatggcagt gatttccatg agcccagagc
    25561 tcttttttga gcaaaatgat cgcgagttga caacacgacc aatgaagggg acgactcagc
    25621 gtggggtaac tgaccaagaa gatcttgaac aggccagttg gttggaacag gatcccaaaa
    25681 atcgctctga aaatatgatg attgtggacc tcttgcgcaa tgatatgaac cgtatttctg
    25741 aagttgggag cgagcacgtg gagcgtctgt gccaggtaga gcagtattca actgtttggc
    25801 agatgacttc gaccatcaag agtcagttgc gagaggatgt ggaccttgtt gaaatcttcc
    25861 gctcactctt tccttgtggt tccataacgg gtgcaccgaa aattgcgaca atggagatta
    25921 tcaagaactt ggagcctcaa ccgcgtggag tctactgtgg aacgattggt ctcttgcttc
    25981 caaatggacg acggattttt aatgtggcca ttcgtaccat tcaacttcac aaaggtcaag
    26041 ccatttatgg agttggcgga gggattactt gggatagcac atgggaatct gaataccgag
    26101 aggttcatca aaaggcagca gttctttatc gtaaacaagc tcgcttccaa ctgattacga
    26161 caggggaaat cagccagaag aacctgctgt ttgaagatca acatctggaa agactgagaa
    26221 aagctagtcg ttattttgcc tttccttttg atgcagaaga cttgggacac aagattgagg
    26281 aagagtgtca ggattgtgaa gctaatcaag attaccgctt gcgaatcagc cttagcaaat
    26341 ctggagagat agaagtcaat cgtcaagtat taacccctct cagtacaagc ttttgtcagg
    26401 cccaagtctg ccttcaggaa gctgctttga atcaatcctt tacctacttt aaaaccactc
    26461 accgaccgca tttgagccta ggagaacaag agaagattta ccacaataag tcaggagaac
    26521 tgcttgaaac ctctatagga aatttggttc tgaaaatcgc tggaaaactc tacacaccgc
    26581 ctatccgact tggaatcttg ccaggaattt accgtcagca tttgctagaa acaggacagg
    26641 tagaagagaa agtcttgacc ttggcagatt taacccaagc agaagctatt tacggctgta
    26701 atgcagtgag aggcttgtat gaactaagcc ttgaggagaa ctagatgaaa ttgatatttt
    26761 tgcatgggtt agggcagtca gcagagtctt ggaaagaagt tcggaatcta ctgacagatt
    26821 atccttctga ggctatcgag ttatttcctt ctggagttag taactatcaa caagctaaag
    26881 agcgagttta tcagcaccta gctcaagaga cagaaccttt tgttttgatt ggattgtcct
    26941 taggtgctgc cctgacacta gaactttcca gttacgattt accaaatctt cgagctttga
    27001 ttctgtcagg atgcccgctt aaattagcag ggaatatcct attttatctt cagttgctga
    27061 tttttaaact acttcccaaa agagtatttg aaaaacaggg agcagacaag actcttatgg
    27121 tcggagtttc tgaggaattg aagacacttg atttaacgga tatagcaggg acttgctcct
    27181 atccaacctt actaatttgt ggcagcaaag ataaaccgaa tcttagttct atgaaagctc
    27241 tccatagact gttaacagat tcgcagtttc agatcattcc tgatggccct catgtcttga
    27301 ataaggcaaa accaaaggag tttgtagaaa agaccagaag tttccttgaa ttgctgaaat
    27361 aagtttgata aaataatatt gaaatcgata tcataattat gggagaaaga aatgaataaa
    27421 cgtctatttt taaaaatgag tctggtgacg ttgccaattt tagccttgtt ttcacaacca
    27481 gttttggcgg aagaaaacat ccatttttcg agctgtaagg aagcttgggc gaatggctat
    27541 tcggatattc acgagggaga acctggttat tctgccaagt tagaccgtga tcatgatggt
    27601 gtggcttgcg aattgaaaaa tgctcctaag ggtgctttta aagcaaaaca gtcaacggct
    27661 attcaaatca acacaagttc agcaacaaca agtggttggg ttaagcagga cggcgcttgg
    27721 tactactttg atggaaatgg aaatctagtg aaaaatgcat ggcagggaag ctattacctg
    27781 aaagctgatg gtaaaatggc acagagtgaa tggatttatg actcttctta tcaagcttgg
    27841 tattatttga catcagatgg ttcttatgca aaaaatgcat ggcaaggagc ttattacctt
    27901 aaatcaaacg gtaaaatggc acaaggtgag tgggtttatg attcttctta ccaagcatgg
    27961 tattacttga aatcagatgg ttcatatgct cgcaatgcat ggcaaggaaa ctactatttg
    28021 aaatcagatg gtaaaatggc taaaggtgaa tgggtttatg atgccaccta tcaagcttgg
    28081 tattatttga catcagatgg ttcttatgct tacagtacat ggcaaggaaa ttactatcta
    28141 aaatcggatg gtaaaatggc tgtcaatgaa tgggttgatg gtggacgtta ttatgttggc
    28201 gctgacggag tttggaagga aggtcaagca agtacagttt cttctagtaa tgatagcaat
    28261 agtgaatatt ctgctgcttt aggaaaggca aaaagttata attcgttatt ccacatgtca
    28321 aaaaaacgta tgtatagaca attaacttct gattttgata aattttcaaa tgatgcagct
    28381 caatatgcca ttgatcattt agatgattaa cagatactgg taggcgaaaa aattccttat
    28441 aattcattta cgcaaacctt tgcataagtg aatggatttt gatatactag tactgtaagc
    28501 attttcaatt actacacaat gaaaaaggag aatatgatga gtcaaaagat tattgggatt
    28561 gaccttggtg gaacttctat caaatttgca atcttaacaa cagtaggaga aatccaagga
    28621 aaatggtcaa tcaagaccaa cattttggat gagggaagtc atatcgttga tgatatgatt
    28681 gagtctattc aacatcgttt ggacttgctt ggattggcag cagcggactt ccaaggcatt
    28741 ggaatgggat caccaggtgt ggttgaccgt gacaaaggga ctgttatcgg tgcctacaac
    28801 ttgaactgga aaacccttca accaatcaaa caaaagattg aaaaagcttt gggcattcca
    28861 tttttcatcg ataatgatgc caacgtagca gctcttggtg agcgctggat gggtgctgga
    28921 gataaccaac cagacgttgt ctttatgaca ctcggtactg gtgttggtgg tggtattgtc
    28981 gcagaaggca aattgcttca tggtgttgct ggtgcagcag gtgaacttgg tcatatcact
    29041 gttgactttg accaaccaat ctcatgtact tgtggtaaaa aaggctgcct tgagacagtt
    29101 gcttcagcaa cagggattgt caacttgact cgtcgctatg ccgatgaata cgaaggcgat
    29161 gcagccttga aacgcttgat tgataacgga gaagaagtaa ctgctaagac tgtctttgat
    29221 ctcgcaaaag aaggagacga ccttgctttg attgtttacc gtaacttctc acgttacttg
    29281 ggaatcgctt gtgctaacat tggttcaatc ctaaacccat caacaatcgt catcggtggt
    29341 ggtgtgtcag ctgcgggaga attccttcta caaggtgttc aaaaagttta cgatgaaaat
    29401 agtttcccac aagtacgcac atctactaaa ttagctcttg caactctagg aaatgacgct
    29461 ggagttatcg gagcagcatc acttgtattg caataagata atgagagagg aaaatgctaa
    29521 cctagagtta gtaatgttcc tcttttattt tttatgctat actagttagg acaatgaatg
    29581 aggtgaaacg aaatgacaaa agcagatacg atttttaaag agaatattga acgcattcta
    29641 aaggaaggtg ttttctcaga gcaggctcgt cctaagtaca aggatgggac tgttgctaat
    29701 tccaagtatg taactggtgc cttttccgag tacgacttat ctaaaggaga atttcctatc
    29761 acaaccttgc gtcccattgc aatcaaatcc gccatcaaag aagttctctg gatttaccaa
    29821 gaccagtcaa atagcttaga agtgcttaat gacaagtaca atgttcacta ctggaatgac
    29881 tgggaagttg gagacacggg aaccattggt gagcgctatg gtgccgttgt taagaaacac
    29941 gacattatca ataagcttct caaacagttg gaaaccaatc cttggaaccg ccgcaatatt
    30001 atttcgctct gggattacca agctttcgaa gaaacagatg ggctgctccc gtgcgccttt
    30061 cagaccatgt ttgatgtccg ccgtatagat ggagaaatct atctggatgc gaccttgacc
    30121 cagcgctcga atgatatgct ggtggcccac cacatcaacg ctatgcagta tgtggctttg
    30181 cagatgatga ttgccaaaca ttttggctgg aaggttggga agttcttcta cttcatcaac
    30241 aacctccata tctatgataa tcaatttgaa caagctcagg aattgctccg tcgggagccg
    30301 tcaaactgcc aaccacgctt ggttttaaat gttcctgatg ggactaattt ctttgatatc
    30361 aaagcagaag attttgagtt ggtggattat gaccctgtta agccacagtt gaagtttgac
    30421 ctagctattt aaaagaatag aaaaaagaag ttgagaataa tcccaacttc ttttgtttct
    30481 taacgtgata ctcggcgacg agctgctttt ttacggtttt cttcgatgaa agctgctttt
    30541 tgctcttctg gttcgattac tttcttttta aatgcgtata ctgcacctgc aacggcagcg
    30601 acagttcctg cgacacctgt tacaagacct ttagcgaatc ctttagccat gagtcttcct
    30661 cctttatatt ctcaatcagc cagcctcctc aagaggtcac atttttctga ctgacctttt
    30721 tgtgttataa taatagtaac gaaaaaatgg gaatttttca aggaaaaaag atgaaaacaa
    30781 aaataattgt gattgttgga ccgactgctg ttggaaagac ggctctagct attgaagttg
    30841 caaagcgttt taatggcgaa gtggttagtg gagatagcca acaagtctat cgaggacttg
    30901 atattgggac agctaaggct agtccagaag agcaggcagc tgttcttcat catttaatcg
    30961 atgttagaga gataaccgag tcttactcgg cttttgattt tgtttcagaa gctaagatga
    31021 ctattgagga tattcacagc cgtggcaagc tagccattat cgccggtggg actggacttt
    31081 atatccagag cttgctagaa ggttaccacc taggtgggga gactcctcat gaggagattt
    31141 tagcttatcg agctagtttg gagccgtatt cagatgagga attagcccat tttgtggagc
    31201 aagcaggcct tgagattccc cagtttaatc gtcgtcgtgc tatgcgtgcc ttagaaattg
    31261 cccattttgg tcaggatttg gaaaatcaag agaccttgta tgaaccgctg attatctgct
    31321 tggatgatga acgtagtcaa ctttatgagc gtatcaacca ccgagtggat ttgatgtttg
    31381 aggctgggct tttggatgag gccaagtggc tgtttgacca ttcccctaat gtacaggctg
    31441 ctaaaggaat tggctataag gaactctttc cttatttccg tggagagcag acctttgagg
    31501 aagctagtga gagtcttaaa caggcgaccc gtcgttttgc caagcgtcag ctgacctggt
    31561 tccgtaatcg catgcaggtc accttttatc agattggaga atctggtgta caagaccgca
    31621 ttttaagcca gatagaggag tttttagatg attgaaacgg agaaaaaaga ggagcgagtc
    31681 ctgctgattg gtgtggaatt gcagggtatg gacagttttg acctctccat ggaagaattg
    31741 gctagtttag cgaaaacggc aggggcagtc gttgtagata gctacagaca aaaacgtgaa
    31801 aaatatgatt ccaagacctt cgtcggctct ggtaagttgg aagagattgc gcttatggtg
    31861 gatgcagaag aaatcactac tgtcatcgtc aacaaccgtc tgaccccaag gcagaatgtc
    31921 aatctagagg aagttctcgg tgttaaggtc attgaccgta tgcagttgat tttggatatc
    31981 tttgccatgc gggctcgaag ccatgaaggg aagttccaag tccacctagc ccaactcaaa
    32041 taccttttgc ctcgcttggt tggtcagggg attatgctca gccgtcaggc agggggaatt
    32101 ggttcccgtg gtcctggtga aagccagctg gagctgaacc gtcgtagcgt tcgcaatcaa
    32161 atcacggata tcgagcgcca gctcaaggtg gttgagaaaa atcgtgcgac tgtcagagaa
    32221 aaacgtttgg agtctagcac ttttaagatt ggtttgattg gttataccaa tgctgggaaa
    32281 tcaactatca tgaacatctt gaccagtaag acccagtatg aagcagatga gctctttgcg
    32341 actctggatg cgacaaccaa gagtattcat ctgggaggca atctccaagt aactttgaca
    32401 gataccgttg gctttatcca agatttgccg acagagttgg tgtccagttt caagtcaacc
    32461 ttggaagaaa gcaagcatgt ggaccttctg gttcatgtta tcgatgctag caatccttac
    32521 cacgaggagc atgaaaaaac ggttctctcc atcatgaaag acctggacat ggaagatatt
    32581 cctcacttga cgctttataa taaagcggat ttggtggagg atttcacgcc tacccaaacg
    32641 ccatataccc tcatttctgc caagtctgag gacagtcgtg aaaacttgca agcattattg
    32701 ctagataaga ttaaggaaat ttttgaagca tttaccctgc gagtgccttt ttcaaagtcc
    32761 tacaagattc atgatttaga gagtgttgca attctggaag aacgtgatta tcaggaagac
    32821 agcgaagtga ttacaggcta catttccgaa aaaaataaat ggaggttaga agaattttat
    32881 gactgatatt aaagctttgg ctctaaagta tggaggttat acaagtctgg acaaggtcta
    32941 tctggttcag cttctagctg gtaaaacaga gcaggagcag ttaacactta ttacacctcc
    33001 gccaagtgtt gttaatgcct actttgcaga actctatcag aaaaagagtc ctcaggctgc
    33061 gacggattat tttgcagaac tcagtcagga actgaatctt tacaatactg agccaagttt
    33121 caccctagaa aataagcctt ttattcggct taatctgtct ggtaaatcct ttggtttttg
    33181 ttatgatagt gacggccttg gtcggatttt ctctgaaaat gaggagaaaa tctcggatga
    33241 cttgctcttt gaaattgcgc aaattttccc ccatcaatta gtctttgaag agtctggtaa
    33301 gatttacatg aagcctgtcg aggacgagga agttgtcagc ctagaaagtc tcacagcttt
    33361 gactgatttg gaaagattgg ctgatggtcg aaaacgtctc aaaggctaca gccaagagga
    33421 tttattgcaa gaagctgctg ctttttctgg caaacgctat ttccgatcgg aaaaccgcac
    33481 agccatgtta tatattgatt aattagaaag tatcgaatgg atattcaatt tttaggaacg
    33541 ggggctggtc agccctctaa agctcgcaac gtttcaagtc tcgccctgaa acttttggat
    33601 gagattaacg aagtttggct ctttgactgt ggagaaggta cgcaaaatcg cattctggaa
    33661 accacagttc gaccacgtaa gatcagcaaa atctttatta cccatctgca tggagaccac
    33721 atttttggtt tgccaggttt cctttctagc cgtgcctttc aggccaatga agagcagaca
    33781 gatttggaaa tctacggacc tcaaggaatc aagtcatttg tcttaaccag ccttcgtgtg
    33841 tcaggttctc gtctgcccta ccgcattcat ttccatgagt ttgaccaaga ttctctagga
    33901 aaaatccttg agaccgataa attcactgtg tatgcagagg agctggacca tactattttc
    33961 tgtgttggct atcgtgtcat gcaaaaggat ctagaaggga cgctggatgc tgaaaaactc
    34021 aaggctgctg gtgttccgtt cggcccgctt tttggtaaaa tcaaaaacgg ccaggatctt
    34081 gttttggaag acggaactga aatcaaggcg gctgactata tctcagcgcc acgtccaggt
    34141 aagattatca ctattttagg agacactaga aaaaccaatg ctagtgtgcg tctggctgtc
    34201 aatgcagatg tcctagttca tgagtccact tatggcaagg gtgatgaaaa aattgctcgt
    34261 aaccatggtc actcaactaa tatgcaagct gcacaagtag cggtagaagc aggtgccaaa
    34321 cgcctcctac tcaaccatat cagtgcccgt ttcctctcaa aagatattag caaactcaag
    34381 aaagacgctg ccacaatttt tgaaaatgtc catgtggtca aagacttgga agaagtggaa
    34441 atctagcagt cacagaaagg ataagtatgc ctactattct cattaccgga gctagcggtg
    34501 gtctagctca agaaatggtc aaactcctgc ccaatgacca actcatcttg cttggtagaa
    34561 ataaggaaaa attagcccaa ctctacggaa attattccca tgcagaattg attgaaattg
    34621 atattaccga cgattcagcc ctagaagctc tggtaactga tctttatctc cgctatggca
    34681 agattgatgt cttgattaac aacgctggtt acgggatttt tgagggattt gaccagattg
    34741 ctgataaaga cattcaccag atgtttgagg tcaatacctt tgccctgatg aatctgtctc
    34801 gtcaccttgc ggctcgtatg aaggaaagca gcaaagggca tatcatcaac atcgtcagca
    34861 tggcaggtct aatagctact ggcaagtcta gtctttactc agcgaccaag tttgcggcta
    34921 ttggtttttc aaatgctctg cgactcgaac ttatgcccta tggagtctat gtgacaacag
    34981 tcaatccagg tccaatccga acaggatttt ttgaccaagc tgacccagat ggaacttatc
    35041 ttaaatcggt tgaccgcttc ctcttagagg cagatgcagt ggctaaaaag attgtcaaga
    35101 ttataggaaa aaataaacga gaactcaatc tcccgatttt gttgaaccta gcccataagt
    35161 tttatactct ctttcccaag ctagctgata agttggcagg ggaaactttt aattataagt
    35221 aaaaagaacc aatgtgcagg ttgttgctag cctacatatt ggttcttatc atttctcaac
    35281 tatcaaactg aacttcttct agtagatagt cgataaagcg ttcgcccatc ttagataggc
    35341 tggttttctc atgctggata tagaccagct caatcgggtc atcaatatcc agtgggatag
    35401 aaacgatatt gtctccgttt aggttgctgt tcaaaatccc tgtcgcaatg gtataaccat
    35461 ccaaaccaat caagagatta aagagggtgg cacggtcact gaccacaatg gatttcttgt
    35521 ggtgttcttg agaaagaatc tcttctgaaa agtagaagga gttgtgcgtc ccttggtcat
    35581 agctgaggta agggaaattc tccaaatcag acagtttcac cttgtctttc tttgccagag
    35641 ggttggtctt gctgacaaag atatgcggtt gcgctgtgaa gagatggtgg gatagcaggt
    35701 gattgtcatc cagcatcttg gttaaaacat cacggttgta actgtttaag aagaggaccc
    35761 cgacctcact gcggaagttc ttgacgtcgt cgataatctc ccaagtccga gtttcacgaa
    35821 ggaagagttc gtatttctcc atatcgcttt tcttgagcaa agagacaaag gcattgacca
    35881 caaaggcata gtgttgagac gaaacgctaa agagttcgcg gtgggcgaca ggatttttat
    35941 agcgttcctc cagaagctga gtttgctcga caacctgacg ggcataagag agaaactcca
    36001 tcccatcacg ggtcaaggta atacccttgg gattgcggat aaagatttca atgcccattt
    36061 cattttccaa atctcgcact gcattggaga gactgggctg agtgataaag agttgcttgg
    36121 ctgcctcatt catagagcca gtttcgacga ttttgataat ataatgtaat tgttgaattc
    36181 tcatgttttt attgtaccac acttgcctta gaatcgccaa agaagatagg aaaataagga
    36241 gcattgtgtg agtctcttct tgaaaaaaac acaaaatttt gctagaatga gtcttatgaa
    36301 aacattctat gatgtgcagc aattcctcaa acgatttggt attattgttt acatgggaaa
    36361 acgcttatat gatattgaac tgatgaagtt ggaactatct cggatttacg atgcagggtt
    36421 gatggataaa ttagactatc tagaggcaga agcagttctt cgcagagagc acaaggtaga
    36481 attggattat attgagaaaa atggagaaaa gaactaatgg ttacttggat tttgtgggca
    36541 cttatactag caatgttggc gtggatgggc tttaactatc ttcgtattcg ccgtgcggct
    36601 aaaattgtgg acaatgagga gtttgaagcc ttgattcgta cgggtcaatt gattgatttg
    36661 cgcgacccag cagaattcca cagaaaacat atccttcgtg cacgcaatat tccttcaagt
    36721 cagttgaaaa ctagtcttgc agcccttcgt aaagataaac ctgtccttct ctacgagaac
    36781 caacgtgcgc aacgagttac aaatgcagct ctttacttga aaaaacaagg tttttctgag
    36841 atttatatcc tttcttatgg cttggattct tggaaaggga aagtgaagac tagctaacct
    36901 tatttccaaa ttagtcgaat gataggatga agctattgtt ttatagtata attaatttgt
    36961 ataaaattta aagagttctt agaacatgaa agaaaagaag aaaaatccac tttttgtggg
    37021 tatcttgtcg attattcttg gtttgttatt tccaattgta ggcctgattt tgggtatcat
    37081 aggattggtc ttggctattt cataccaaaa agagtcacaa ttagagtata aaatagaaaa
    37141 gattcttaat atcttaggaa ttgtgatttc tgtagttaac tggattgtag caatcgcctt
    37201 aatttttcgg taaataaaag aaaaaagctt tagttttaaa gctttttttg ttttatcgac
    37261 tcatctctaa atagttttta atgatttgta actctttttg gttcaaaggg cggtagttac
    37321 cttctgctaa atctgggttc aatgtgaagt ccccaaattg gattcttttg aggctagtca
    37381 ccttaacacc aaccgagagg aacattttct tgatttgatg aaattttcct tctgaaatgg
    37441 tgatagaggc ttgactgagg gagggacttg cagatagaat ctctagtttt gcgggtttac
    37501 agacagtacc atctaaaaag acaattccct tttgaaaggc ttggatatgg tcaggtgtta
    37561 gaagtccatt aacctcaact tgataagtct tatcgacatg atattgggga tggaggagtt
    37621 ggaagcccaa gggaccgtta tcggtcaaga gaaggagtcc tgttgtatct cggtccagtc
    37681 ggccgacggc atagagcttg tcagactgga tgtcagatgg aagcaagtcc atgacggtcg
    37741 gaagttcctt gtctttgttg gctgtaacga caccagcagg tttatgaagc ataagatagg
    37801 tgtgttcata gccttgaatg attcgatcct gaaaaaggag ttcttgtagt cctgtatcga
    37861 tattttgagc tagggagcgg gctgggcaac catcgactag aatttcccct ctgaggagtg
    37921 cttgcttcat ggcctttcgg ctaacttttt cttgggctaa taaattatct aaacgcatac
    37981 tgtgcctatc ttatcataag tcgcttgctt tgaaaaggct tgacgtgaaa agaaaagcgg
    38041 agtgaaaaca tttacacttg cttgagttat gttatttatt tgaaattatg gtataatcgt
    38101 tcagttagaa aataaatttt gaatattata gaggaaatca tgacaaaatt aagagaagat
    38161 atccgtaaca ttgcgattat cgcccacgtt gaccacggta aaacaaccct ggttgacgaa
    38221 ttattgaaac aatcagaaac gcttgatgca cgtactgaat tggcagagcg tgctatggac
    38281 tcaaacgata tcgaaaaaga gcgtgggatt accatccttg ctaaaaatac tgccgttgct
    38341 tacaacggaa ctcgtatcaa cattatggac acaccaggac acgcggactt tggtggagaa
    38401 gttgagcgta tcatgaaaat ggttgacggt gttgtcttgg tcgtagatgc ctatgaagga
    38461 accatgccac aaactcgttt cgtattgaaa aaagccttgg aacaagacct tgtcccaatc
    38521 gtggttgtta acaaaatcga taagccatca gctcgtccag cagaagtagt ggatgaagtc
    38581 ttggaacttt tcatcgagct tggtgcagat gacgaccagc ttgatttccc agtggtttat
    38641 gcttcagcga tcaacggaac ttcttcattg tcagatgatc cagctgacca agaagcgact
    38701 atggcaccaa tctttgacac gattatcgac catatcccag ctccagtaga taactcagat
    38761 gagcctttgc agttccaagt gtcacttttg gactacaatg acttcgttgg acgtatcggt
    38821 atcggtcgtg tcttccgtgg tacagttaag gttggggacc aagttaccct ttctaaactt
    38881 gacggtacaa ctaaaaactt ccgtgttaca aaactcttcg gtttctttgg tttggaacgt
    38941 cgtgaaatcc aagaagccaa agcgggtgac ttgattgccg tttcaggtat ggaagacatc
    39001 tttgtcggtg aaaccatcac tccgacagat gcagtagaag ctcttccaat cctacacatc
    39061 gatgagccaa ctcttcaaat gactttcttg gtcaacaact caccatttgc tggtaaagaa
    39121 ggtaaatggg taacttctcg taaggtggaa gaacgcttgc aggcagaatt gcaaacagac
    39181 gtttcccttc gtgttgaccc aactgattca ccagataaat ggactgtttc aggacgtgga
    39241 gaattgcact tgtcaatcct tatcgaaaca atgcgtcgtg agggctatga acttcaagta
    39301 tctcgtccag aagttatcgt aaaagaaatc gacggtgtta aatgtgaacc atttgaacgt
    39361 gtacaaatcg acactccaga agaataccaa gggtctgtta tccaaagcct ttctgaacgt
    39421 aaaggtgaaa tgttggatat gatttcaact ggtaatggtc aaactcgttt ggtcttcctt
    39481 gttccagcgc gtggtttgat tggatactca actgagttct tatcaatgac tcgtggttac
    39541 ggtatcatga accatacctt cgaccaatac ttgccattga ttccagggga aattggtgga
    39601 cgtcaccgtg gtgcccttgt ttctatcgat gctggtaagg ctacaactta ctcaatcatg
    39661 tctatcgaag aacgtggtac gatctttgtc aacccaggta ctgaggttta tgaaggaatg
    39721 atcatcggtg aaaactctcg tgaaaatgac ttgacagtta acatcacgaa ggcaaaacaa
    39781 atgaccaacg ttcgttcagc tactaaggac caaacagctg ttatcaagac gcctcgtatc
    39841 ttgacacttg aagagtctct tgagttcttg aacgacgatg agtacatgga agtaacgcct
    39901 gagtctatcc gtttgcgtaa acaaatcctt aacaaagcag agcgtgagaa agctaacaag
    39961 aagaaaaaat cagctgaata agagctagaa agagagaaag atggtctatt tagtcctagg
    40021 aattttactg ctcctactct atgtatttgc gacaccagaa agcattaaag ggactgtcaa
    40081 tatcgtcgct atggtatgta ttttagtggc actcttgatt ttattggttc tatcttttct
    40141 gaaaattttt caattaccaa cagaaatatt cctagcaata gccatgttga tcctagctta
    40201 ctttagtgtt agagacatca cactcatgcc agtcaaaaaa agtaaaagaa gataaaaaag
    40261 agagtttcgg ctctcttttt gctaggaatt ttacaatagt ttgaattgct ttttagttaa
    40321 caccatctct tttacttgta tagtatgaat gggaaaaact tgaataaaat tcgttaatgg
    40381 catctatttt atatttggtt agtactggtt gcatattatt ttga
//
LOCUS       DAVLVZ010000013        40312 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000013 DAVLVZ010000000
VERSION     DAVLVZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40312
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.013"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            5..75
                     /locus_tag="VYN77_000623"
     tRNA            5..75
                     /locus_tag="VYN77_000623"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VYN77_000624"
     tRNA            110..183
                     /locus_tag="VYN77_000624"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VYN77_000625"
     tRNA            266..355
                     /locus_tag="VYN77_000625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            369..442
                     /locus_tag="VYN77_000626"
     tRNA            369..442
                     /locus_tag="VYN77_000626"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Met,seq:cat)
     gene            446..518
                     /locus_tag="VYN77_000627"
     tRNA            446..518
                     /locus_tag="VYN77_000627"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:479..481,aa:Phe,seq:gaa)
     gene            531..611
                     /locus_tag="VYN77_000628"
     tRNA            531..611
                     /locus_tag="VYN77_000628"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:565..567,aa:Tyr,seq:gta)
     gene            616..686
                     /locus_tag="VYN77_000629"
     tRNA            616..686
                     /locus_tag="VYN77_000629"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:648..650,aa:Trp,seq:cca)
     gene            701..773
                     /locus_tag="VYN77_000630"
     tRNA            701..773
                     /locus_tag="VYN77_000630"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:734..736,aa:His,seq:gtg)
     gene            784..855
                     /locus_tag="VYN77_000631"
     tRNA            784..855
                     /locus_tag="VYN77_000631"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:Gln,seq:ttg)
     gene            865..948
                     /locus_tag="VYN77_000632"
     tRNA            865..948
                     /locus_tag="VYN77_000632"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:899..901,aa:Leu,seq:caa)
     gene            1055..2331
                     /locus_tag="VYN77_000633"
                     /pseudo
     CDS             1055..2331
                     /locus_tag="VYN77_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
     gene            2407..3891
                     /locus_tag="VYN77_000634"
     CDS             2407..3891
                     /locus_tag="VYN77_000634"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1558124.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     NKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNTQGQYELTDFDAEILELFVAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEALTGKVGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTIVAAADMQVAVEAYLGL"
     gene            3952..5232
                     /locus_tag="VYN77_000635"
     CDS             3952..5232
                     /locus_tag="VYN77_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1558125.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMNLKG"
     gene            5236..5856
                     /locus_tag="VYN77_000636"
     CDS             5236..5856
                     /locus_tag="VYN77_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1558126.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6022..7164
                     /locus_tag="VYN77_000637"
     CDS             6022..7164
                     /locus_tag="VYN77_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1558127.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPAEKQETQTSPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7570..7908
                     /locus_tag="VYN77_000638"
     CDS             7570..7908
                     /locus_tag="VYN77_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1558128.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            7919..8287
                     /locus_tag="VYN77_000639"
     CDS             7919..8287
                     /locus_tag="VYN77_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV1558129.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYNALLGVFLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            8394..9004
                     /gene="pcp"
                     /locus_tag="VYN77_000640"
                     /pseudo
     CDS             8394..9004
                     /gene="pcp"
                     /locus_tag="VYN77_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(9065..9922)
                     /locus_tag="VYN77_000641"
     CDS             complement(9065..9922)
                     /locus_tag="VYN77_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1558130.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYQA
                     VLARKRPKA"
     gene            10052..11194
                     /gene="tgt"
                     /locus_tag="VYN77_000642"
     CDS             10052..11194
                     /gene="tgt"
                     /locus_tag="VYN77_000642"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1558131.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11292..13109
                     /locus_tag="VYN77_000643"
     CDS             11292..13109
                     /locus_tag="VYN77_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1558132.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPSYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHRMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13262..14413
                     /gene="nagA"
                     /locus_tag="VYN77_000644"
     CDS             13262..14413
                     /gene="nagA"
                     /locus_tag="VYN77_000644"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1558133.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14576..15634
                     /locus_tag="VYN77_000645"
     CDS             14576..15634
                     /locus_tag="VYN77_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1558134.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15785..16150
                     /locus_tag="VYN77_000646"
     CDS             15785..16150
                     /locus_tag="VYN77_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1558135.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16226..17167
                     /locus_tag="VYN77_000647"
     CDS             16226..17167
                     /locus_tag="VYN77_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1558136.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17115..18131
                     /locus_tag="VYN77_000648"
     CDS             17115..18131
                     /locus_tag="VYN77_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1558137.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVSRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18133..18459
                     /locus_tag="VYN77_000649"
     CDS             18133..18459
                     /locus_tag="VYN77_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1558138.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18452..18856
                     /locus_tag="VYN77_000650"
     CDS             18452..18856
                     /locus_tag="VYN77_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1558139.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18861..19121
                     /locus_tag="VYN77_000651"
     CDS             18861..19121
                     /locus_tag="VYN77_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558140.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19084..19545
                     /locus_tag="VYN77_000652"
     CDS             19084..19545
                     /locus_tag="VYN77_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1558141.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19523..19969
                     /locus_tag="VYN77_000653"
     CDS             19523..19969
                     /locus_tag="VYN77_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1558142.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNTLNPE"
     gene            20072..20666
                     /locus_tag="VYN77_000654"
                     /pseudo
     CDS             20072..20666
                     /locus_tag="VYN77_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20727..21680
                     /locus_tag="VYN77_000655"
     CDS             20727..21680
                     /locus_tag="VYN77_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1558143.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELEQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQVNHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21731..22921
                     /locus_tag="VYN77_000656"
     CDS             21731..22921
                     /locus_tag="VYN77_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1558144.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23070..23441
                     /gene="rnpA"
                     /locus_tag="VYN77_000657"
     CDS             23070..23441
                     /gene="rnpA"
                     /locus_tag="VYN77_000657"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1558145.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23416..24240
                     /locus_tag="VYN77_000658"
     CDS             23416..24240
                     /locus_tag="VYN77_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1558146.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24259..25245
                     /locus_tag="VYN77_000659"
     CDS             24259..25245
                     /locus_tag="VYN77_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1558147.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSRTFYVTINVNDYVEHRAEVLQTYAQKL
                     ATRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25296..25919
                     /locus_tag="VYN77_000660"
     CDS             25296..25919
                     /locus_tag="VYN77_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1558148.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLEIPTVKGIYYLTYSDGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26050..27507
                     /locus_tag="VYN77_000661"
     CDS             26050..27507
                     /locus_tag="VYN77_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1558149.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFIFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27530..27811
                     /locus_tag="VYN77_000662"
     CDS             27530..27811
                     /locus_tag="VYN77_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1558150.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27888..28373
                     /locus_tag="VYN77_000663"
     CDS             27888..28373
                     /locus_tag="VYN77_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1558151.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28390..29055
                     /locus_tag="VYN77_000664"
     CDS             28390..29055
                     /locus_tag="VYN77_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1558152.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29059..29922
                     /locus_tag="VYN77_000665"
     CDS             29059..29922
                     /locus_tag="VYN77_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1558153.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDIYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29924..30628
                     /locus_tag="VYN77_000666"
     CDS             29924..30628
                     /locus_tag="VYN77_000666"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1558154.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYRAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30807..32480
                     /locus_tag="VYN77_000667"
     CDS             30807..32480
                     /locus_tag="VYN77_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1558155.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFALIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNESKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32592..33683
                     /gene="ulaG"
                     /locus_tag="VYN77_000668"
     CDS             32592..33683
                     /gene="ulaG"
                     /locus_tag="VYN77_000668"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1558156.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33797..35773
                     /gene="tkt"
                     /locus_tag="VYN77_000669"
     CDS             33797..35773
                     /gene="tkt"
                     /locus_tag="VYN77_000669"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1558157.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35890..36189
                     /gene="yajC"
                     /locus_tag="VYN77_000670"
     CDS             35890..36189
                     /gene="yajC"
                     /locus_tag="VYN77_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1558158.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36235..36663
                     /locus_tag="VYN77_000671"
     CDS             36235..36663
                     /locus_tag="VYN77_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1558159.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36665..37075
                     /locus_tag="VYN77_000672"
     CDS             36665..37075
                     /locus_tag="VYN77_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558160.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37375..40026
                     /gene="adhE"
                     /locus_tag="VYN77_000673"
                     /gene_synonym="adhC"
     CDS             37375..40026
                     /gene="adhE"
                     /locus_tag="VYN77_000673"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1558161.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELIKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12202 a         7227 c         9099 g        11784 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attattaacg cgggatggag cagctcggta gctcgtcggg ctcataaccc gaaggtcgta
      421 ggttcaaatc ctgctcccgc aataaggctc ggtagctcag ttggtagagc aatggattga
      481 agctccatgt gtcggcggtt cgattccgtc tcgcgccatt tatatatttt ggaagggtag
      541 cgaagaggct aaacgcggcg gactgtaaat ccgctccttc gggttcgggg gttcgaatcc
      601 ctccccttcc attttacggg catagtttaa aggtagaact aaggtctcca aaaccttcag
      661 tgtgggttca attcctactg cccgtgttaa tagaattatg gcgggtgtgg tgaagtggtt
      721 aacacaccag attgtggctc tggcatgcgt gggttcgatc cccatcactc gcctatttta
      781 tattggggta tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga
      841 atccagctac cccagttact atttgccggc gtggcggaat tggcagacgc gctggactca
      901 aaatccagtg tccgcaagga cgtgccggtt cgaccccggc cgccggtata gtatagtgtt
      961 aggaacgttg ttattcttcg ttcctttttt atattatttt tggtataatt atagttattc
     1021 aaattttatt tagattaaga aagtgtaggg gagtatgtct tgttctatcg atttattaaa
     1081 acatcggtat ttgaaaaata ttaaagaaaa tcctgaattg tttgtcggaa ttgagttgga
     1141 gtatcctgtt gcaagtttag aaggggatgc tacagatgtt gaagttataa aggatctatt
     1201 tcattattta gtttctactt tggatctcac cgtagcaaag gtagatgatt ttggcaatct
     1261 gatccagtta gtagatccga taagtcagga tgctatttta tttgaagttt cctatacaac
     1321 gattgagttt gcatttggta aggctgaaac gattcaagag gtcgaaaatc gtttcaataa
     1381 ttatatgaat gtaattcaga gaaagttagc tgaatcaaat catgctattg ttggctgtgg
     1441 tatccatccc aactgggata aaaatgagaa ttgtccagtg gcttatccac gctatcagat
     1501 gttgatggat tatttgaatt tgagtagaaa tattattaaa tcagatttac atcatttccc
     1561 tgaatatggt acttttatct gtgggagcca ggttcagctg gatatttcaa aaaccaacta
     1621 cttgcgggtg attaatgctt ttactcaaat tgaagcggct aaggcttatt tatttgcaaa
     1681 ctctgagttt tcgggtgcgg attgggatac gaaaatttca agggatattt tctgggaaga
     1741 atctatgcat ggtatctatc cagagaatgt tggggtcaat gctagactcc ttaatgatga
     1801 agctgatttt ttgactatct aaatcattct gcgattttta ctgcggaacg tgatgggcag
     1861 acctattatt tttatcctat tcaggctggg gactatttgg ctacgcctga aatccaagca
     1921 tttgctctga atggggatga ggttattatt tacccccaag agaaggattt tgaaactcat
     1981 cgtagttacc agtaccaaga cttaacgact cgaggaacag ttgagtttcg tagtgtgtgt
     2041 acacagccac ttgataggac ttttgcttct gcagcttttc acttgggatt attggttaat
     2101 ttagacaagt tagaagctta cttagaaaca gcacctttct ttaaagtatt tggttatgat
     2161 tacaagtttt taaggagaca attttctaag aaaaatctta cagatgagga agaaactatg
     2221 attattgaat tttccaaaga cttactccta ctagctgagg agggactagt ggtgagaaat
     2281 aaggaagaaa tgacctattt acagcctttg agagaagaat tgagcctata atttctctta
     2341 taaagggaga attttctgaa aaatcatgat ataatggacg agactataga taaaggatag
     2401 agagtaatga cattagttta tcaatcaacg cgtgatgcca acaatacagt aactgccagc
     2461 caagcaattt tgcaaggttt ggcgacggac ggcggtttgt ttacaccggt tacttatcca
     2521 aaggtagatt tgaactttaa caaattgaaa gatgcttctt accaggaagt tgctaagcta
     2581 gttttgtcag catttttaga tgactttaca gctgaggagt tggactactg tatcaacaat
     2641 gcctacgata gcaaatttga tactccagct attgcaccat tagtgaaatt agatgggcaa
     2701 tacaatttgg aacttttcca tggttcaacg attgccttta aggatatggc cttgtctatt
     2761 ttgccatact ttatgacgac tgctgctaag aaacatggtt tggagaacaa gattgttatc
     2821 ttgacagcga catctggtga cacggggaaa gctgctatgg cggggtttgc ggatgtgcct
     2881 ggtactgaga ttatcgtctt ttatccaaag gatggtgtca gcaagattca agagttacaa
     2941 atgaccactc aaactggcga caatactcat gttattgcta ttgatggtaa ctttgacgat
     3001 gcgcaaacaa atgtgaagca catgtttaac gacgtggctc ttcgtgaaaa attgactacc
     3061 aacaagttgc aattttcatc agctaactct atgaacattg gtcgtctggt gccacaaatt
     3121 gtttattatg tttatgctta cgctcaattg gtcaagactg gtgagatcgt ggctggtgat
     3181 aagattaact tcacagtacc aacaggaaac tttggaaata tcttggctgc cttttatgcc
     3241 aaacaaatcg gtctcccagt tggtaaattg atctgtgctt caaatgacaa caatgttttg
     3301 acagacttct tcaagacacg tgtttacgac aagaaacgtg agtttaaggt aacaaccagc
     3361 ccatctatgg atatcttggt atcttcaaac ttggagcgtt tgattttcca tcttttgggg
     3421 aatgatgcgg ttaagacggc tgaactcatg aatgccttga atacacaagg acaatatgaa
     3481 ctgactgatt ttgatgcaga gattctggag ctctttgtag ctgaatatgc gactgaggaa
     3541 gaaacagcgg cagaaatcaa gcgtgtttat gagttagatt cttatattga ggatccacat
     3601 acggcggttg cctcagcagt ttatagaaaa taccaagcgg ttactgatga tgcgactaag
     3661 acagtgattg cttcaacagc tagtccctac aagttcccag tagttgcagt agaagcgcta
     3721 acagggaaag ttggcttgac tgattttgaa gctttggctc aattacatga aatctcaggc
     3781 gttgcagtgc caccagcagt tgatgggctt gagacagctc ctgttcgtca caagacaata
     3841 gtggcagctg ctgacatgca agtagcggtt gaggcttatt taggacttta agatagaggg
     3901 agtaaactcg gttgggaaac caactgagtt tcttttcatc aggaggagag attgtttaag
     3961 aaaaataaag acattcttaa tattgcattg ccagctatgg gtgaaaactt tttgcagatg
     4021 ctaatgggaa tggtggacag ttatttggtt gctcatttag gattgatagc tatttcaggg
     4081 gtttcagtag ctggtaatat tatcaccatt tatcaggcga ttttcatcgc tctgggagct
     4141 gctatttcca gtgttatttc aaaaagcata gggcagaaag accagtcgaa gttggcctat
     4201 catgtgactg aggcgttgaa gattacctta ctattaagtt tccttttagg atttttgtcc
     4261 atcttcgctg ggaaagagat gataggactt ttggggacgg agagggatgt agctgagagt
     4321 ggtggactgt atctatcttt ggtaggcgga tcgattgttc tcttaggttt aatgactagt
     4381 ctgggagcct tgattcgtgc aacgcataat ccacgtctgc ctctctatgt tagtttttta
     4441 tccaatgcct tgaatattct tttttcaagt ctagctattt ttgttctgga tatggggata
     4501 gctggtgttg cttgggggac aattctgtct cgtttggttg gtcttgtgat tttgtggtca
     4561 caattaaaac tgccttatgg gaagccaact tttggtttag ataaggaact gttgaccttg
     4621 gctttaccag cagctggaga gcgacttatg atgagggctg gagatgtagt gatcattgcc
     4681 ttggtcgttt cttttgggac ggaggcagtt gctgggaatg caatcggaga agtcttgacc
     4741 cagtttaact atatgcctgc ctttggcgtc gctacggcaa cggtcatgct gttggcccga
     4801 gcagttggag aggatgattg gaaaagagtt gctagtttga gtaaacaaac cttttggctt
     4861 tctctgttcc tcatgttgcc cctgtccttt agtatatatg tcttgggtgt accattaact
     4921 catctctata cgactgattc tctagcggtg gaggctagtg ttctagtgac actgttttca
     4981 ctacttggga cccctatgac gacaggaaca gtcatctata cggcagtctg gcagggatta
     5041 ggaaatgcac gcctcccttt ttatgcgaca agtataggta tgtggtgtat ccgcattggg
     5101 acaggatatc tgatggggat tgtgcttggt tggggcttgc ctggtatttg ggcagggtct
     5161 ctcttggata atggttttcg ctggttattt ctacgctatc gttaccagcg ctatatgaac
     5221 ttgaaaggat aggaaatgca aaaaacagct tttatttggg atttagacgg gactttattg
     5281 gactcttacg aagcgatttt atcagggatt gaggagactt ttgctcagtt ttctattcct
     5341 tatgataagg agaaggtgag agagtttatc ttcaagtatt cggtgcaaga tttgcttgtg
     5401 cgggtggcag aagatagaaa tctggatgtt gaggtgctaa atcaggtgcg tgcccagagt
     5461 ctggctgaga agaatgctca ggtagttttg atgccaggtg cgcgtgaggt gctagcttgg
     5521 gcagacgaat caggaattca gcagtttata tatactcata aggggaacaa cgcttttacc
     5581 attctcaagg acttgggggt ggaatcctat tttacagaga ttttaaccag tcagagtggc
     5641 tttgtgcgga agccaagtcc agaagcggct acctatctgc tagataagta tcagttgaat
     5701 tctgataata cttattatat aggggatcgg actctggatg tggaatttgc ccagaatagt
     5761 gggattcaaa gtatcaactt tttagagtct acttatgaag ggaatcacag gattcaagcg
     5821 ttagcagata tttcccgtat ttttgagact aagtgataaa aagattgtgt cagttttgtg
     5881 acagagacct aacaaactat ttcaagtaac ctagtttgtt acaaggaata gacagttctg
     5941 ttaaataggc ccgagagggc tttttttcta cattttttgt gttatcatag acaggtactc
     6001 atttgaaagg aatttgaaag aatgaagaaa agaatgttat tagcgtcaac agtagccttg
     6061 tcatttgccc cagtattggc aactcaagca gaagaagttc tttggactgc acgtagtgtt
     6121 gagcaaatcc aaaacgattt gactaaaacg gacaacaaaa caagttatac cgtacagtat
     6181 ggtgatactt tgagcaccat tgcagaagcc ttgggtgtag atgtcacagt gcttgcgaat
     6241 ctgaacaaaa tcactaatat ggacttgatt ttcccagaaa ctgttttgac aacgactgtc
     6301 aatgaagcag aagaagtaac agaagttgaa atccaaacac ctcaagcaga ctctagtgaa
     6361 gaagtgacaa ctgcgacagc agatttgacc actaatcaag tgaccgttga tgatcaaact
     6421 gttcaggttg cagacctttc tcaaccaatt gcagaagctc caaaagaagt agcatcaagt
     6481 tcagaagtta caaagacagt gattgcttct gaagaagtgg caccatctac gggcacttct
     6541 gtcccagagg agcaaacggc cgaaacaagc agtgcagttg cagaagaagc tcctcaggaa
     6601 acgactccag ctgagaagca ggaaacacaa acaagccctc aagctgcatc agcagtggaa
     6661 gcaactacaa caagttcaga agcaaaagaa gtagcatcat caaatggagc tacagcagca
     6721 gtttctactt atcaaccaga agaaacgaaa gtaatttcaa caacttacga ggctccagct
     6781 gcgcccgatt atgctggact tgcagtagca aaatctgaaa atgcaggtct tcaaccacaa
     6841 acagctgcct ttaaagaaga aattgctaac ttgtttggca ttacatcctt tagtggttat
     6901 cgtccaggag acagtggaga tcacggaaaa ggtttggcta tcgactttat ggtaccagaa
     6961 cgttcagaat taggggataa gattgcggaa tatgctattc aaaacatggc cagccgtggc
     7021 attagttaca tcatctggaa acaacgtttc tatgctccat tcgatagcaa atatgggcca
     7081 gctaacactt ggaacccaat gccagaccgt ggtagtgtga cagaaaatca ctatgatcac
     7141 gttcacgttt caatgaatgg ataaacccga cttgataaca tcattttgac gaatgagatc
     7201 tagctttcgt gatggaaagc gattctcgtt cgttttttct ttgtcatact cttcgaaaat
     7261 ctcttcaaac cacgtcagtt ttatctgaaa cctcaaagct gtgctttgag caacctgcga
     7321 ctagctttct agtttactct ttgattttca ttgagtatca atttgaatgg aaaatggaaa
     7381 gttattatct tgtaataatc caagcaacat tcttgcaatc tattttactt tatatcacaa
     7441 ttgattggtc aactattgat aagtcaatgg ataggaggaa gaaatgatag agattcaaga
     7501 tttactgtat caactccgct tgtctgagca agcgagtacg caattgtttg aaaaaggctt
     7561 gggattagtt tgacacggta tcagatttta ctgtttttgc tggagcattc tccttgtaac
     7621 caaatggcgg ttcaggagcg tttgaaaatt gatcaggctg ctttgacacg gcatttcaag
     7681 attttggaaa cggaaggttt ggtggagcgt catcgtaatc ctgaaaatca gcgggaagtg
     7741 ttggtagagg ctgcgaagta tgccaaggag cagttagtgg tgaatccccc tctgcaacat
     7801 atcaaggtta aggaagagat ggaaagtatc ttaacagagt ttgagagaac agaactcagc
     7861 cgtttattaa ataaattggt tttgggtatt gaaaatatag aaatttaagt agaaatagat
     7921 gtcaattatt acaattattt taacaacgat cgttgctttg gagcattttt acatttttta
     7981 tttggaaagt attgccacgc aatcagatgc gactagtcgt gtatttaata tggaaaagga
     8041 agaattggct catccgtcag taagttcatt gttcaaaaat caaggaattt ataacgctct
     8101 gctaggagtc tttctcttgt atggcattta tttctcacag aatttagaaa ttgtgactat
     8161 ttttgtctta tttgtgattg gtgctgcgac ttacggctct ttaacagcgg ataaaaaaat
     8221 tattttgaaa caaggtggat cagctatttt ggccttgatt agtattttac tctttaaata
     8281 cacttgaagg tcgattctaa tctcgctaat cctttttaat ccagaataag ggaaatatgt
     8341 tatacttgtt tttaagaaaa aagtctcatt gaattggttt tgaggagtta gaaatgaaag
     8401 tattagtgac aggttttgag ccctttggag gggaaaaggg caatccagct ttggaggcca
     8461 ttaaaggttt accagctgaa atccatggtg ctgaggtccg ttggctagag gtgccgacag
     8521 tttttcacaa atctgctcaa gtattggaag aagagatgaa tcgttatcaa cctgactttg
     8581 tcctttgtat tgggcaagct ggtggaagaa ctagtttgac acctgaatga gtggccatta
     8641 atcaagacga tgcacgcatt tctgataacg aagataatca accgattgac cgtcccattc
     8701 gcccagatgg tgcttcggcc tactttagta gtttgccgat taaagcgatg gttcaagcta
     8761 taaaaaagaa ggattaccgg cctctgtttc caatacggca gggacttttg tctgcagcca
     8821 tttgatgtat caggctctct atttggtaga aaagaaattt ccatatgtta aggcaggttt
     8881 tatgcatatt ccttatatga tggaacaggt ggtgaacaga ccgactactc caactatgag
     8941 tttagtggat attcggcgag ggatagaagc agcaatcggc gctatgatag aacatggaga
     9001 ttaggaactc aagttggtag gcggagaaac tcattgatag aaaaaagctt gaggggaaaa
     9061 accttcaagc ttttggacgt tttcgagcca atactgcttg gtaaaacata attttagtgc
     9121 attggatata aggtaggagt gaaaaactag caatgccaaa ggtaatccaa ttgaggaagt
     9181 accaaggaag aagctgtaaa tctaggacaa agtgctggaa cttgtagccc ttcataaagg
     9241 aacggctagt ttttaggatt cgtcttggtg ggacctgtcc taggtctaga ctataacaga
     9301 gaagaaattc cacctgtgaa taggcataat actgtggaat atagaggata ttccctacaa
     9361 tgatcaagat gagacttgca agaaagtaga gtccaaagac catgaggaaa cgctcggttt
     9421 caactgatga gagatctaga tttggaaact caggatgtag ggtgacgaat tttttggcta
     9481 aaaagctact ataaaagagg aggtaaatcc caagtaaatt agggatactc cataaaaaga
     9541 gatagaaacg tttgagaagt agggtcaaaa aggtttgaga aaagcgctcc tcatcaaaga
     9601 gagctaggct gttttttaca gatggctccg ttttagaatc tttcatgagt gtcagtgttg
     9661 catagacgga actggtcaaa agaatagtcc cgataaagga gactagtaga ggaaagaggt
     9721 aggcttgaag tatttggcca agtatgctga aaaatggctg ttctaaaaca gtcccgtgga
     9781 tccgagataa gggattaaga aaactagata agatgaccag catactggga aggatataga
     9841 ggagaaagag acggggggtg tcagcctgaa aatgttttga ctcctgacga attgttttta
     9901 aatcaatttt tggatagttc attctcttat tataccatag ttcttataca tagttcgtga
     9961 cagttcctac tttttttgat aaaatcatac agtgtgtcct tgggcacact gtatgaactg
    10021 ggactgtctt tcccagcttc ggaggtaaaa aatgtcagat tcaccaatca aatatcgttt
    10081 gattaagaaa gaaaaacaca caggagctcg tctaggagaa atcatcactc cccacggtac
    10141 ctttccgaca cctatgttta tgccagttgg gacacaagcc actgtcaaaa ctcagtcacc
    10201 tgaagaattg aaggagatgg gttcgggaat tatcctatca aacacctatc atctctggct
    10261 tcgccctgga gatgaactca ttgcacgcgc tggtggtctc cacaagttca tgaattggga
    10321 ccagcctatc ttgacagata gtggtggttt tcaggtttat tctttagcag atagccgtaa
    10381 tatcacagaa gaaggagtaa cctttaaaaa tcatctaaat ggttctaaga tgttcctatc
    10441 cccagaaaaa gccatctcta ttcagaataa tctgggttca gacatcatga tgtcctttga
    10501 tgaatgtcct cagttttatc aaccttatga ctacgttaag aaatcgatcg agcgtaccag
    10561 ccgttgggct gagcgtggtt tgaaggctca ccgtcgtcca catgaccaag gtttgtttgg
    10621 aattgtgcaa ggtgcaggat ttgaagacct tcgccgccaa tcagctcatg atcttgtcag
    10681 catggatttc tcaggctact ctatcggtgg tttggcagtg ggagaaaccc atgaagagat
    10741 gaatgcggtc ttggacttta caactcaact gctacctgaa aataaacctc gttatctgat
    10801 gggtgtggga gcgccagata gcttgatcga tggggtcatt cgtggggtgg atatgtttga
    10861 ctgtgtctta ccgactcgaa ttgctcgtaa cgggacttgt atgaccagtc aaggacgttt
    10921 ggttgtgaaa aatgcccagt ttgctgagga ctttacgcca ctggatcctg agtgtgactg
    10981 ctacacatgt aataactata cacgcgctta ccttcgtcac ctgctcaagg ctgatgaaac
    11041 ctttggtatc cgcttgacta gctaccacaa tctttacttc ttgcttaacc tgatgaagca
    11101 agtgcgacaa gccatcatgg atgacaatct cttggaattc cgtgagtatt ttgtggaaaa
    11161 atatggctat aataagtcag gacgtaattt ctaaaatgga attgatataa aaaaatccta
    11221 agttttctct taggattttt cttctttttt tgatagaata aagtgtacaa tgaaaggaag
    11281 aataaactcg tatgcgcatt aaatggtttt ccttgattag gattataggt ttacttttgg
    11341 tactcttgta tcacttcttt cagacgatct ttcctggagg atttttcggg gtagatgtct
    11401 ttttcacatt ttcaggtttc ctgattacag ctctactcat tgaagaattt tctaagaaca
    11461 atgagattga tttgatagga ttttttagaa gacgctttta tcggattgtg ccacctgtgg
    11521 ttttgatggt cttggtgacc atgcctttta ctttcttggt tcgccaagac tatgttgctg
    11581 gaattggtgg ccagattgcg ggcgtcttag gctttatgac caacttctat gaactcctaa
    11641 caggtgggag ttatgaatct cagttcattc ctcatttgtt tgttcataat tggagtttgg
    11701 cagttgaggt tcactactat attctttggg gattggcagt ttggttctta tccaaacaag
    11761 ctaaatcaaa tggtcagttg aaggggatgg tctttctctt atctgctgct gccttcttga
    11821 tcagtttctt ctccatgttt attggtagtt ttctagtgac ctcttattcc tctgtttatt
    11881 tctccagttt aactcatgtc tatccattct ttttgggaag tatgttagca actattgtag
    11941 gcgttcgtca gacgacttcc ctcgtcaagc agttggataa aatctgggat ttacgaaaga
    12001 ctttggtagt ttttggagga ggctttggtt tcctagttct tttgactttc tttgtcaaat
    12061 tcacttatct ttttgcctat cttatcggct tcttgcttgc cagtcttgca gctcttgcca
    12121 tgattctggc ggcgcgtgtc ttacatgaaa agacacatca tatacaggag ccgaagatta
    12181 tcagcttttt agcggatact agctatgcgg tttatctttt ccattggcct tcttatatca
    12241 ttttctcaca gttgacatca aatcttcttg ctgtattact gactctgatt tgttcttatg
    12301 gctttgccag tctgtcattc tatgtattgg aaccttggat tgcaggcaag aacacaccta
    12361 ttttacaaac tcttcgtccc ctgccttata ttcacgcaat tcttgcagca ggtacaggaa
    12421 tcttgacctt cattgtctgc atggtgacct tgttggcacc acaagtggga gcgtttgaga
    12481 cagacttgac tgttaatggc ttgaagcaag ctgcaacaaa tattggccag accaaggtga
    12541 tggcagaacg ggcagatgca aacagtttgg gaattgctga tggcactatg ttaattggtg
    12601 actcagtggc tttaagggca aatacagcac tacagacagc tcttcctgga gcacagatta
    12661 acgcgcaggt cagcgtaaca accaagaccg caaatgaaat catgctaaat aatagccaga
    12721 ataaattttt acctaagacg gtggtcattg cgactggggt aaataatcct gagaattaca
    12781 aggatgactg ggacagtatc gtgaaaaatc ttcctaaggg acaccgtatg attttggtga
    12841 ctccttatga gggagataag acaaaagaga cctatgccat cgttgagaag gctgctgcct
    12901 atatgagaga attggcagag aagacacctt acattacgat agcagattgg aatcaagttg
    12961 cgaaagagca tccagaaatt tgggcaggaa cagaccaggt tcatttcggg agtgagagta
    13021 gcactatcga agcaggagca aaattgtatg cagatacgat tgccacagct ttgcagacag
    13081 ctcaagacaa gccggttaaa tcaaaataac ttgtattaaa aaagagaaga gttggagaaa
    13141 tccagctctt ttaaaatatc tccaaaaaat aggtctatac catttacaaa taaaaaagaa
    13201 aggtttataa tgtaattgac ataataaatt gtagaatcaa tcttttaagg aggttaacat
    13261 tatgcctaac tatattaaag cggatcagtt tttctaccca cacggagttc gtcgaggtgg
    13321 ttacttggaa cttgtggacg gcaagtttgg gaaacatgta gagcagattc ctgaaggggc
    13381 tgaggtgatt gactatacag gttatagcat tgccccaggt cttgtggata ctcatattca
    13441 tggatatgca ggtgtagatg tgatggacaa caacattgaa ggtacattgc atactatgag
    13501 tgaaggactt cttagtaccg gtgttaccag tttcttaccg acaactttaa cagccactta
    13561 tgagcaattg cttgcagtca ctgaaaatct tggaaaccat tataaagaag caacaggtgc
    13621 taagattcgt gggatttatt atgaaggtcc atatttcaca gaaactttta agggggcaca
    13681 aaatccaact tatatgagag acccgggtgt tgaggagttt cattcttggc aaaaagcggc
    13741 aaatggcttg cttaataaaa ttgcccttgc accagaacgt gatggggtgg aagactttgt
    13801 tcgtacagtt acgggcgaag gtgtgacggt tgctcttgga cattcaaacg cgacttttga
    13861 tgaagccaaa aaagcagtcg atgctggagc gagtgtttgg gtgcatgcct acaatggaat
    13921 gcgtgggttg actcaccgtg aattgggtat ggttggagcc atgtaccaat tgccacatac
    13981 ctatgcagag ttgatctgtg atggtcacca cgtagatcca aaggcttgcg aaattcttat
    14041 caaacaaaaa ggaacagaaa acatcgctct tatcacagac tgtatgacag ctgggggatt
    14101 ggaagacgga gattatatgt tgggagaatt cccagttgtc gttgcaaatg gaactgcacg
    14161 cctcaaatcg acaggtaact tggcaggttc tatcctcaaa ctcaaagatg gtttgaagaa
    14221 tgtggtcgaa tggggaattg cgaatccgca tgaagcagtc atgatggcca gcttcaaccc
    14281 agctaaatcc gttcacatcg atgacgtctg tggccaaatc cgtgaaggct acgacgctga
    14341 cttcatcgta ttagataaag atttggaatt ggtagcaacc tacctagatg gcgtgaaacg
    14401 ttatcaagca taagagagaa ggcagaagtt agaaactagc ttctgctttt ttatacaggg
    14461 tactttactg gtaaataaaa agttaaaaaa atcacaaaaa aagcttgaag aaacaaagaa
    14521 aaaggcttac aattgaacta taaatattac aaataaaaaa acggaggagt gctttatgaa
    14581 agcctatact tatgttaaac caggacttgc ttcttttgtt gatgtagaca aaccagttat
    14641 tcgcaagcca acagacgcta ttgtgcgtat tgtaaaaacc actatttgtg gaacagacct
    14701 ccatattatc aaaggggatg ttcctacttg ccaaagtggt accattcttg gccacgaagg
    14761 gattgggatt gttgaagaag ttggggaagg agtttccaac ttcaaaaaag gtgacaaggt
    14821 cttgatttct tgcgtctgtg cctgtggtaa atgctactac tgtaaaaaag gaatttatgc
    14881 ccactgtgaa gacgaagggg gctggatttt cggtcacttg attgatggta tgcaggctga
    14941 atatctacgt gtccctcatg cagataatac tctttaccat actccagaag acttgtcaga
    15001 tgaagctttg gttatgctgt cagacattct gcctactgga tatgaaattg gtgtcttaaa
    15061 agggaaagta gaacctggtt gcagcgtagc cattattggt tcaggtccag ttggattggc
    15121 tgctctttta acagcccaat tctattcacc agctaaattg attatggtag acctagacga
    15181 taaccgcttg gaaactgccc tatcattcgg tgcgactcat aaggttaatt cttcagaccc
    15241 tgaaaaagcc attaaagaaa tttatgattt gacagatggt cgtggtgtgg atgtcgctat
    15301 cgaagctgtt ggtattcctg aaacatttga tttctgtcaa aagattatcg gtgtagacgg
    15361 aacggttgcc aactgtggtg tgcatggtaa accagttgaa ttcgatttag ataaactttg
    15421 gattcgcaac atcaatgtaa caactggttt ggtatctaca aatacgactc cacaattgtt
    15481 gaaagcactt gaaagtcata agattgaacc agaaaaattg gtaactcact atttcaaact
    15541 cagtgaaatt gaaaaagcct atgaagtctt cagtaaggca gcagaccacc atgccattaa
    15601 ggtcattatc gaaaacgata tctcagaagc ctaagtagta aaaatatttt tgtacataag
    15661 taaatagaaa ttcagtcatc catcagatgg ctggattttt tatcaaaaaa ttaagaaatg
    15721 agcatatttc tttccttgtc tggcggaatt ggttataata tacggtacaa aggaatgaat
    15781 gaatatgtat cgtgttatag aaatgtacgg agattttgaa ccgtggtggt tcttagaagg
    15841 ttgggaagaa gatattgtag caagtagaaa atttgaccag tattatgatg ctctcaaata
    15901 ctacaaaact tgctggttta gattggaaca agaatcccct ctttataaaa gtagaagcga
    15961 cttgatgacc attttttggg acccggaaga ccaacgctgg tgtgatgaat gtgatgagta
    16021 tttacaacaa taccattctt tggctctttt gcaggatgag caggttatcc cagacgaaaa
    16081 actacgctca ggctatgaaa aacaaaccag tcaggaaagg aatcgttctt gccgtatgaa
    16141 attaaaatag agaaaagtaa cttttttgga gttgcttttt ttatttttct aacgctttgc
    16201 gaatagtata ggtgaggagg taagtatggt tcaagaaatt gcacaagaaa tcattcgttc
    16261 agctcggaaa aaagggacgc aggatattta ttttgtccct aagttagacg cctatgagct
    16321 tcatatgagg gtaggagacg agcgctgtaa aattggtagc tatgattttg aaaagtttgc
    16381 agccgttatc agtcacttta agtttgtggc gggtatgaat gtgggagaaa aaagacgtag
    16441 tcaactgggt tcctgtgatt atgcctatga ccagaagatg gcgtctctac gtttatctac
    16501 tgtaggcgat tatcgggggc atgagagttt ggttatccgt ttgttgcacg atgaggagca
    16561 ggacttgcat ttttggtttc aggatattga agaattaggc aagcagtaca ggcaacgggg
    16621 actctatctt tttgctggtc cggttgggag tggtaagacg accttgatgc atgaattgtc
    16681 caagtcactc tttaaaggac agcaagttat gtccatcgaa gatcctgtcg aaatcaagca
    16741 ggacgacatg cttcagttgc agttgaacga agcaatcggc ctaacctatg aaaatctaat
    16801 caaactttcc ttgcgtcatc gaccagatct cttgattatc ggagaaattc gtgacagcga
    16861 gacggcgcgt gcagtggtca gagctagttt gacaggtgcg acagtctttt caaccattca
    16921 cgccaagagt atccgaggtg tttatgagcg tctgctggag ttgggtgtga gtgaagaaga
    16981 attggcagtt gttctgcaag gagtctgcta ccagagatta atcgggggag gaggaatcgt
    17041 tgactttgca aacagagatt atcaagaaca ccaagcagcc aagtggaatg agcaaattga
    17101 ccagcttctt aaagatggac atatcacaag tcttcaggct gagacggaaa aaattagcta
    17161 cagctaagca aaaaaatatc atcaccctat ttaacaatct cttttctagc ggttttcatc
    17221 tggtggagac tatctccttt ttagatagga gttccttgtt ggacaagcag tgtgtgaccc
    17281 agatgcgtac aggcttgtct caggggaaat cattctcaga aatgatggaa agtttgggat
    17341 gttcaagtac cattgtcact cagttatctc tagctgaagt tcatggcaat cttcacctga
    17401 gtttgggaaa gatagaagaa tatctagaca atctggccaa ggtcaagaaa aagttaattg
    17461 aagtagcgac ctatcctttg attttgctgg gttttcttct cttaattatg ctggggctac
    17521 ggaattacct gctcccacaa ctggatagta gcaatattgc cacccgaatt atcggtaatc
    17581 tgccacaaat ttttctaggc atggtagggc ttgtttccgt gcttgccctt ttagcactaa
    17641 ctttttataa aagaagttct aagatgagtg tcttttctat cttagcacgc cttcccttta
    17701 ttggaatctt tgtgcagacc tacttgacag cctattatgc acgtgaatgg gggaatatga
    17761 tttcacaggg aatggagctg acgcagattt ttcaaatgat gcaggaacaa ggttcccagc
    17821 tctttaaaga aatcggtcaa gatctggctc aaaccctgaa aaatggccgt gaattttctc
    17881 agacgatagg aacctatcct ttctttagga aggaattgag tctcatcata gagtatgggg
    17941 aagttaagtc caagctgggt agtgagttgg aaatctatgc tgaaaaaact tgggaagcct
    18001 tttttacccg agtcagccgc accatgaatt tggtgcagcc actggttttt atctttgtgg
    18061 cactgattat cgttttactt tatgcggcaa tgctcatgcc catgtatcaa aatatggagg
    18121 taaattttta aaatgaaaaa aatgatgaca ttcttgaaaa aagctaaggt taaagctttt
    18181 acattggtgg agatgttagt agtcttgctg attatcagcg tgcttttctt gctctttgta
    18241 cctaatctga ccaagcaaaa agaagcagtc aatgacaaag gaaaagcagc tgttgttaag
    18301 gtggtggaaa gccaggcaga actttatagc ttggataaaa atgaagatgc tactctgagc
    18361 aagttacaag gagatgggcg catcacggaa gaacaggtta aagcttataa tgaatactat
    18421 actaaaaatg gaggggcaaa tcgtaaagtc aatgattaag gcctttacca tgctggaaag
    18481 tctcttggtt ttgggacttg tgagtatcct tgccttgggc ttatccagct ctgtccagtc
    18541 cacttttgca gcagtagagg agcagatttt ctttatggag tttgaagaac tctatcggga
    18601 aacccaaaaa cgcagtgtag ccagtcagca aaagactagt ctaaatttag atgggcagac
    18661 gcttagcaat ggcagtcaaa agttgacagt tcctaaagga attcaggcac catcaggcca
    18721 aagtattaca tttgaccgag ctgggggcaa ttcgtccctg gctaaggttg aatttcagac
    18781 cagtaaagga gcgattcgct atcaattata tctaggaaat ggaaaaatta aacgcattaa
    18841 ggaaacaaaa aattagggca gtgattttac tggaagcagt agtcgctcta gctatctttg
    18901 ccagcattgc gaccctcctt ttgggacaaa ttcaaaaaaa taggcaagag gaagcaaaaa
    18961 tcttgcaaaa ggaagaagtc ttgagggtag ctaagatggc cctgcagacg gggcaaaatc
    19021 aggtaagcat caacggagtt gagattcagg tattttctag tgaaaaagga ttggaggtct
    19081 accatggttc agaacagttg ttggcaatca aagagccata aggtcaaggc ttttaccttg
    19141 ttagaatccc tgcttgccct cattgtcatc agtgggggat tactcctttt tcaaactatg
    19201 agtcagctcc tcatttcaga agttcgctac cagcaacaaa gcgagcaaaa ggagtggctc
    19261 ttgtttgtgg accaacttga ggcagaatta gaccgttcgc agttcgaaaa agtagaaggc
    19321 aatcgcctat acatgaagca agatggcaag gacatcgcca tcggtaagtc aaagtcagac
    19381 gattttcgta aaaccgatgc tagtggacgg gggtatcagc ctatggttta tggcctcaaa
    19441 tcagctcaga ttacagagga caatcaactg gttcattttc gtttccagtt tcaaaaaggc
    19501 ttagaaaggg agttcatcta tcgtgtggaa aaagaagaaa gttaaggcag gtgttctcct
    19561 ctacgcagtc accatagcag ccatctttag tcttttgttg caattttatt tgaaccgaca
    19621 aatcgcccac tatcaagact atgctttaaa taaagaaaaa ttggttgctt ttgccatggc
    19681 taaacgaacc aaggataaag ccgagcaaga aagtggggaa caggttttta atctaggtca
    19741 ggtaagctat caaaacaaga aaacaagctt ggtgacgact gttcgtacgt ctaagagcca
    19801 atatgagttt ctgtttcctt cagtcaaaat caaagaagag aaaagagata aaaaggaaga
    19861 ggtagcgacc gattcaagcg aaaaagcgga gaaaaaaaat cagaagagaa gcctgaaaag
    19921 aaagagaatt cctagtcaat tcaactataa tacgttgaat ccagaatagt ccactgtagt
    19981 ttctagaaaa ttgctggaaa tggatgttaa gctccaattc atttgtttat atcttatttc
    20041 agtttactat actttgtgct aaattaaaga tatgaaacat gattttaacc acaaagcaga
    20101 aactttcgat tcccctaaaa atatcttcct cgcaaacttg gtatgtcaag cagccgagaa
    20161 acagattgat cttctatcag acaaagaaat tttagatttc ggtggtgaca cgggtctatt
    20221 agccttgccc ctagccccta gccaagcagg ctaagtcagt cactcttgta gacatttctg
    20281 agaaaatgtt ggagcaagct cgtttgaaag tggagcagca agcaatcaag aatatccagt
    20341 ttttggagca agatttaccg aaaaatccct tggagaaaga gtttgatttc cttgctgtta
    20401 gtcgggttct tcatcatatg cctgatttgg atgcggctct ctcactgttt catcaacatt
    20461 tgaaggaaga tgggaaactc atcattgctg attttaccaa gacagaagct aatcatcatg
    20521 gatttgattt agctgaactg gaaaacaagc taattgagca tggtttttca tctgtgcata
    20581 gtcagattct ctatagcgct gaagacctgt ttcaaggaaa tcactcagaa ttctttttaa
    20641 tagtagccca aaaatcactc gcctagtcag ggagtgattt ttctataagg atggaaaaaa
    20701 gaagggaaat ttgataagat aggaatatgg attttgaaaa aattgaacaa gcttatatct
    20761 atttactaga gaatgtccaa gtcatccaaa gtgatttggc gaccaacttt tatgacgcct
    20821 tggtggagca aaacagcatc tatctggatg gtgagactga actagaacag gtcaaagaca
    20881 acaatcaggc ccttaagcgt ttagcactac gcaaagaaga atggctcaag acctaccagt
    20941 ttctcttgat gaaggctggg caaacagaac ccttgcaggt caatcaccag tttacaccgg
    21001 atgctattgc tttacttttg gtgtttattg tggaagagct gtttaaagag gaggaaatta
    21061 ctatcctcga aatgggttct gggatgggaa ttttgggcgc tactttcttg acctcgctta
    21121 ctaaaaaggt ggattatttg ggaatggaag tggatgattt gctgattgat ttggcagcta
    21181 gtatggcaga tgtaattggt ttgcaggctg gctttgtcca aggagatgcc gttcgtccac
    21241 aaatgctcaa agaaagtgat gtggtcatca gcgacttgcc tgttggctat tatcctgatg
    21301 atgccgttgc gtcgcgccat caagttgctt ctagccaaga acatacttac gcccatcact
    21361 tgctcatgga acaagggctt aagtacctca agtcagacgg atacgctatt tttctagctc
    21421 cgagtgattt gttgaccagt cctcaaagtg atttgttaaa agaatggctg aaagaagagg
    21481 cgagtctggt tgctatgatt agtctgcctg aaaatctctt tgctaatgct aaacaatcta
    21541 agactatttt tatcctacag aagaaaagtg aaatagcagt ggagcctttt gtttatccac
    21601 ttgctagctt gcaagatgca agtgttttaa tgaaatttaa agaaaatttt caaaaatgga
    21661 ctcaaggtac tgaaatataa aatagatttt gttataatag ttgaaaacgc ttaaaaaggg
    21721 gtatcatgtt atgacaaaaa caattgcaat caatgcagga agttcaagtt tgaaatggca
    21781 attatactta atgccagagg aaaaagtgtt agccaaaggt ttgattgaac gtatcggttt
    21841 gaaagattca atttcaactg taaaatttga cggccgttct gaacaacaaa ttttggatat
    21901 cgaaaatcat acacaagctg ttaaaatttt attggatgac ttgattcgtt tcgatattat
    21961 caaggcttat gacgagatta caggtgttgg ccaccgtgtt gtagcaggtg gagaatattt
    22021 caaagaatca acagttgttg agggagatgt tttagaaaaa gttgaagagt tgagtttgtt
    22081 ggctcctcta cacaacccgg ccaatgcagc aggtgttcgt gctttcaagg aattgttgcc
    22141 agacattacc agtgtagttg tttttgatac ttccttccac acaagtatgc cagagaaagc
    22201 ttatcgctac cctctaccaa caaaatatta cacagaaaac aaggttcgta aatacggtgc
    22261 tcatggtaca agtcaccagt ttgtagcagg agaagctgca aaactcttgg gacgtccatt
    22321 agaagacttg aagttaatta cctgtcatat tggtaacgga ggctcaatta cagctgtgaa
    22381 agccggcaaa tctgtagaca cttctatggg gttcactcct cttggtggta ttatgatggg
    22441 aacgcgtaca ggggatattg atccagctat cattccttat ttaatgcaat atacagagga
    22501 ttttaacaca ccagaagata tcagtcgtgt tcttaaccgt gaatcaggtc ttttgggagt
    22561 ttctgctaat tctagcgata tgcgcgatat agaagcagct gtagcagaag ggaatcacga
    22621 ggctagcttg gcttatgaaa tgtatgttga ccgtatccaa aaacatatcg gtcagtacct
    22681 tgcagtgcta aatggaacag atgccattgt tttcacagca ggtgtcggtg aaaatgcaga
    22741 gaatttccgt cgtgatgtaa tctcagggat ttcgtggttt ggttgtgatg ttgatgatga
    22801 aaagaatgtc tttggcgtta caggagacat ctcaacagag gcagctaaaa tccgtgtctt
    22861 ggttattcca acagatgaag aattagtcat tgcccgtgac gttgaacgct tgaaaaaata
    22921 agtgaaacta aaaaaatatt caatacaagg agttgggaaa gttatttttc cagcttcttt
    22981 ttctgatgaa attgtccaaa accttgctat gattggcttt tttgaaaaat atggtataat
    23041 agtagtaatt taatagatgg agttgagttt tgaagaaaaa ctttcgtgta aaaagagaga
    23101 aagattttaa ggcgattttc aaggagggga caagttttgc taatcgcaaa tttgtggtct
    23161 accaattaga aaaccagaaa aaccattttc gagtaggtct atcagttagc aaaaaactgg
    23221 ggaatgccgt cactagaaat caaattaagc gacggattcg gcatattatc cagaatgcaa
    23281 aagggagtct ggtagaagat gtcgactttg ttgtcattgc tcgaaaagga gtcgaaacct
    23341 tgggatacgc agagatggag aaaaatctac tccatgtatt aaaattatca aagatttacc
    23401 gggaaggaaa tgggagtgaa aaagaaacta aagttgacta gtttgctagg actgtctctg
    23461 ttaatcatga cagcctgtgc gactaatggg gtaactagcg atattacagc cgaatcggct
    23521 gatttttgga gtaaattggt ttacttcttt gcgaaaatca ttcgcttttt atcgtttgat
    23581 attagtatcg gagtggggat tattctcttt acggtcttga ttcgtacagt cctcttgcca
    23641 gtctttcagg tgcaaatggt ggcttctagg aaaatgcagg aagctcagcc acgcattaag
    23701 gcgcttcgag aacaatatcc aggtcgagat atggaaagca gaaccaaact agagcaggaa
    23761 atgcgtaaag tatttaaaga aatgggtgtc agacagtcag attctctttg gccgattttg
    23821 attcagatgc cggttatttt ggccttgttc caagccctat caagagttga ctttttaaag
    23881 acaggtcatt tcttatggat taaccttggt agtgtggata caacccttgt tcttccgatt
    23941 ttagcagcag tattcacctt tttaagtact tggttgtcca acaaagcttt gtctgagcga
    24001 aatggcgcta cgactgcgat gatgtatggg attccagtct tgatttttat ctttgcagtt
    24061 tatgcgccag gtggagtcgc cctatactgg acagtgtcta atgcttatca agtcttgcaa
    24121 acctatttct tgaataatcc attcaagatt atcgcagagc gcgaggccgt agtacaggca
    24181 caaaaagatt tggaaaatag aaaaagaaaa gccaagaaaa aggctcagaa aacgaaataa
    24241 ataaggagga atctggtaat ggtagtattt acaggttcaa ctgttgaaga agcaatccag
    24301 aaaggattga aagaattaga tattccaaga atgaaggctc atatcaaagt catttctagg
    24361 gagaaaaaag gctttcttgg tctatttggt aaaaaaccag cccaagtgga tattgaagcg
    24421 attagtgaaa cgactgttgt taaagcaaat caacaggtag taaaaggcgt tccgaaaaaa
    24481 atcaatgatt tgaacgagcc tgtgaagacg gttagtgaag aaaccgttga ccttggtcat
    24541 gtggttgatg ctattaaaaa aatagaggaa gaaggtcaag gtatttctga tgaagtcaag
    24601 gctgaaatct taaaacatga aagacatgcc agcactatct tagaagaaac tggtcacatt
    24661 gagattttaa atgaacttca aatcgaggaa gcgatgaggg aagaagcagg cgctgatgac
    24721 cttgaaactg agcaagatca aactgaaaat caagacttga aagagatggg cttgaaggtc
    24781 gagcaaagtt atgatattgc ccaggtggct acggatgtga ctgcctatgt tcaagcgatt
    24841 gtggatgaca tggatgttga agctacactt tcaaatgatt ataaccgtcg tagcatcaat
    24901 ctacaaattg acaccaacga accaggtcgt attatcggct accatggtaa agtcttgaag
    24961 gccttgcaac tgttggctca aaattatctt tacaaccgct attccagaac cttctacgtt
    25021 acaatcaatg tcaatgatta tgtcgaacac cgtgcagaag tcttgcagac ctatgcgcaa
    25081 aaattggcga ctcgtgtttt ggaagaaggg cgcagtcata aaacagatcc aatgtcaaat
    25141 agcgaacgca agattatcca tcgtattatt tcacgtatgg atggcgtgac tagttactct
    25201 gaaggtgatg agccaaatcg ctatgttgtt gtagatacag aataagtaaa atcaggttta
    25261 tcctgatttt ttgctagtta gaggaggtta aactgatgtt gaataagata agagactatt
    25321 tagactttgc tggtttgcag taccgtaatc ctgataaagc gggagcagag cgagagaaga
    25381 tgctggcatt ccgccacaaa ggacaagagg cccgaaaggc ttttacagaa ctggccaaag
    25441 cctttcaagc aagccatcca gaatggcaac tccaacagac tagccagtgg atgaatcagg
    25501 cccagcgttt gagaccacat ttttgggttt atctacagag agacggacaa gtgacagaac
    25561 ctatgatggc cttacgtttg tatgggacat ctactgactt tggaatttct ttggaagtca
    25621 gtttcatcga acgtaagaag gatgagcaaa cactgggcaa gcaggccaaa gttttagaaa
    25681 ttccaaccgt taaagggatt tattatctaa cctactctga tggtcaaagt caacggtggg
    25741 aggcgaatga agaaaagcgt cgtactttac gcgagaaggt gagaagtcaa gaagttcgaa
    25801 aagttttagt gaaggtagat gttcctatga cagaaaattc gtctgaagaa gaaatcgtag
    25861 aaggcttatt gaagtcttat tctaaaattc ttccctatta tctagctacg agaaaataag
    25921 ataatttgta aaacatcata aatcatacag tccaagagtg aacagtccgc tgtgtaattc
    25981 ttggtctttt tgtttgcgct ttcgcattat ataataaact tacaaaaaca attcaaaagg
    26041 agaacaatta tggaagtcgt ttcaagtgtt ctaaattggt tttctagcaa tattttgcag
    26101 aatcccgcat ttttcgtagg tttattggtg ttgataggat atgcactttt gaaaaaacct
    26161 gcccatgacg ttttttcagg gtttgttaaa gcaacagtag ggtatatgtt gcttaacgtg
    26221 ggtgctggtg gtttggttac aacctttcgt ccaatcttag cggctcttaa ctacaaattc
    26281 caaattggtg cagcggttat cgacccttac tttggacttg ctgcagcaaa caacaaaatt
    26341 gcagcagagt ttccagattt tgttggaact gcgactacag ctctattgat tggtttcgga
    26401 ataaatatct tgctcgtagc tcttcgaaag attacgaagg taagaaccct ctttattact
    26461 ggtcacatca tggtacaaca agctgcaaca gtatctctta tggttctatt cttagtacca
    26521 caattgcgca atgcttacgg tacagcagcg attggtatca tctgtggact ttactgggca
    26581 gttagttcaa atatgactgt tgaggcaact caacgcttga ctggtggtgg cggatttgcg
    26641 attggtcacc aacagcaatt tgcaatctgg tttgtagata aagtagcagg acgctttggt
    26701 aagaaagaag aaagtttaga caatcttaaa ttacctaagt tcctctcaat cttccacgat
    26761 acagttgttg catctgctac cttgatgctc gtattcttcg gagccattct tttaatcttg
    26821 ggtccagaca ttatgtctaa taaagaagtc atcacttcag gaactctatt caatcctgct
    26881 aaacaagatt tctttatgta cattatccaa acagccttta ccttctcagt ttacttgttc
    26941 gttttgatgc aaggtgtccg aatgttcgta tctgagttga caaacgcctt ccaaggtatt
    27001 tcaaacaaat tgttgccagg ttcattccca gcggttgacg ttgcagcttc ttatggattt
    27061 ggttctccaa atgctgtcct gtcaggattt atctttggtt tgattggtca attgattaca
    27121 attgttttgc tcatcgtctt taaaaatccg attcttatta ttacaggatt tgtaccagtg
    27181 ttctttgaca atgcagccat tgcggtctac gctgataaac gcggcggatg gaaagcggct
    27241 gttatccttt cctttatatc aggtgtcctt caagttgctc taggagctct ttgtgtggcc
    27301 cttctcgatt tggcatctta tggtggctac catggaaata tcgactttga attcccatgg
    27361 cttggatttg gatatatctt caaatacctt ggtattgttg gttatgtact tgtgtgtctc
    27421 ttcttgcttg ttattcctca acttcaattt gccaaagcaa aagataaaga gaaatattac
    27481 aacggtgaag ttcaagaaga agcttagtat ctagaaaagg agaaataaaa tggttaaagt
    27541 attagcagcg tgcggaaatg gaatgggttc atcaatggtt atcaagatga aggttgaaaa
    27601 tgctctccgt aagcttaatc aaacagattt tacagtcaat tcatgcagtg tcggtgaagc
    27661 taaaggttta gcagtaggat atgacatcgt aatcgcttct cttcatttga ttcaagaatt
    27721 ggaagggcga actaatggga agttaattgg gcttgataac ttgatggatg ataaagaaat
    27781 caccgaaaaa ctcagtcaag cactacagta aaaggttgga gggggctgga cagaaactga
    27841 gagttatcgt ttctgtcctt ctccctcttt aaataaagga ggcagatatg aatttaaaac
    27901 aagctttaat tgacaacgac tcgatccgac taggtttaga ggctaacgat tggaaagaag
    27961 cagtcaaggt agcagtagat cccttgattg aaagtggggc aattttgcca gagtattacg
    28021 atgctatcat tgaatcgact gaagagtatg ggccttacta tatcttgatg ccaggtatgg
    28081 ctatgcccca cgctagacct gaagctggtg tgcaaagtga tgccttttca ttaattacct
    28141 tacaaaatcc tgttgtattt tcagatggga aagaggtatc tgttttgttg gcactagcag
    28201 caacaagttc aaaaattcac acaagtgtag ccattccaca aattattgcc ctgtttgaat
    28261 tagaagattc tattgcacgt ttacaggctt gccagactaa agaagatgtc ttggctatga
    28321 ttgaagaatc taaggatagc ccttatctcg aaggattgga tttggaaagt tagaaagagg
    28381 aataaagaaa tgacaaaaag aatacctaat ttacaagttg cattagacca ttcagacttg
    28441 caaggagcga ttaaagcagc tgtttctgtt ggtcaggaag tagatattat cgaagctgga
    28501 actgtttgct tgcttcaagt tggaagtgaa ctggctgaag tcttgcgtag ccttttccca
    28561 gataagatta ttgtggcaga cacaaaatgt gctgatgctg gtggaacagt tgctaaaaat
    28621 aatgcggttc gtggagcaga ctggatgact tgtatctgtt gtgcaaccat ccctactatg
    28681 gaagcagctc taaaggctat caagactgaa cgaggagaac gaggcgaaat ccagatcgag
    28741 ctttatggcg attggacttt tgaacaagct cagctttggc tagatgcagg tatctcacaa
    28801 gctatttatc accaatctcg tgatgctctt cttgctggtg aaacttgggg tgaaaaagac
    28861 cttaataagg ttaaaaaact cattgacatg ggcttccgtg tatctgtaac aggtggtcta
    28921 gatgtagata ctctcaaact ctttgaaggt gttgatgtct ttacctttat cgcaggtcgt
    28981 ggaattacag aggctgcgga tccagcagga gcagcgcgtg ccttcaagga tgaaatcaaa
    29041 cgaatttggg ggtaaatcat ggtacgtcca attggaattt atgaaaaggc aaccccaaca
    29101 cactttactt ggctagaacg tttaaatttt gccaaggagt taggctttga ttttgtcgag
    29161 atgtctattg acgaacgtga cgagcgttta gcaagacttg actggagtaa ggaagaacgc
    29221 ttggaagttg tcaaagcaat ctatgaaact ggtgttcgta ttccttctat ctgtttttca
    29281 ggccatcgtc gctacccatt gggttcaaaa gatccagttc tagaggaaaa atctctagaa
    29341 ctcatgaaaa aatgtatcga attagctcaa gacttgggag ttcgtacgat tcaattagct
    29401 ggttacgatg tttactatga ggaaaagtca ccccagacac gccaacgttt tatcaaaaat
    29461 ttgagaaaag cctgtgactg ggctgaagaa gctcaggtgg tacttgctat tgaaattatg
    29521 gatgatcctt tcatcaatag catcgaaaaa tatttggcta tagaaaaaga gattgactct
    29581 cccttcctct ttgtatatcc agatattggt aatgtgtctg catggcataa tgatatctat
    29641 agtgagtttt atcttggtca tcatgccatc gcagctctcc atctcaagga tatttatgca
    29701 gtgacagaaa gttcaaaggg ccagttccga gatgtacctt tcgggcaagg ttgtgtcaaa
    29761 tgggaagaag ctttcgatat tttaaaggaa accaattata atggaccttt cctaatcgaa
    29821 atgtggtctg aaaattgtga aacagtagaa gaaacacgcg cagccattca agaggcgcaa
    29881 gcttttctct atccactcat taagaaagca ggtttgatgt aagatgaatc aagtaatcaa
    29941 tgctatgcgt aaacgagtct gtgatgccaa tcaatcattg ccaaaacatg gacttgtcaa
    30001 atttacctgg gggaatgtat ctgaagtcaa tcgcgaactc ggtgtcattg ttatcaaacc
    30061 atcaggcgtg gattatgacg aattgacacc tgaaaacatg gtagtgactg atctagatgg
    30121 taagatccta gaaggggatt taagaccatc ttccgacctc ccaactcatg tgcaattata
    30181 tagggcttgg tcagaaattg gtagtgtggt tcacacccat tcgacagaag ctgttggttg
    30241 ggctcaggca ggtcgtgata ttcctttcta cggaacaacc catgcagatt atttctacgg
    30301 ttcaatccct tgcgcccgta gtttgaccaa ggacgaagta gaagtggcct atgaaaaaga
    30361 tactggcctg gttatcgtag aagagtttga acatcgcgga cttaacccgg ttgaagtacc
    30421 aggaattgtt gtacgcaatc acggtccatt cacctggggc aaaaatccag agaatgctgt
    30481 ttatcactct gtcgtactag aggaagtatc aaagatgaat cgctttacag aacaaatcaa
    30541 tccaagagtt ggacctgctc cccagtacat actagaaaaa cactaccaac gtaaacatgg
    30601 accaaatgct tattatggtc aaaagtaaga acgatgaagg aggagaaaaa gataaattta
    30661 gctcctcttt ttacatttga tttttattga gagtaaagtt ggagttgaag taattttaaa
    30721 agatttttta gaaatagcgc ttgatatata tatggtaaaa taaaaagaat tgctgtgata
    30781 tcaatagatt tgggggattt tttaatatgg tactggataa ggcaagttgt gatttgcttc
    30841 aatatttgat ggatcaagaa acgtccaaaa cgattatggc gatttcgaaa gatttgaaag
    30901 agtcaagaag gaaaatttat tatcacattg acaaaatcaa tgctgctctg ggtgacgagg
    30961 cgcttcacat cattagtatt ccacgaattg gtattcactt aacggaagag cagagagatg
    31021 cttgttgtaa actattatcg gaagtagatt cgtacgatta tatcatgagt gcgcatgaac
    31081 gtatgatgat aatgttacta tggataggta tttctaaaga acgtattacg attgaaaaat
    31141 tgatagagtt aacagaggta tctaggaata ctgttctcaa tgatttgaat agtattcgtt
    31201 atcaactaac tttggaacaa tatcaggtga tcttgcaagt gagcaagtca cagggatacc
    31261 accttcatgc ccaccctctt aataaaattc agtatcttca atcgcttcta tatcatattt
    31321 ttatggaaga aaatgccact tttgtatcta ttttagaaga taagatgaaa gagaggttag
    31381 atgatgagtg tttgctttct gttgaaatga accaattttt taaggaacag gttcctttag
    31441 ttgaacaaga tttagggaag aaaataaacc atcatgaaat aacttttatg ttgcaggttc
    31501 taccttattt gctgttaagc tgtcataatg ttgaacagta tcaagaaaga catcaggata
    31561 tagagaaaga atttgctttg ataagaaaaa gaatagagta tcaggtgtct aagaagttag
    31621 gagaacggtt gtttcaaaag tttgaaattt ctttgtcagg acttgaagtt tctcttgtag
    31681 ctgttctcct cctctcctat cgtaaagatt tggatattca tgcagaaagt gatgattttc
    31741 ggcaattaaa acttgcttta gaagaattta tctggtattt tgaatcacaa atccgaatgg
    31801 agattgagaa caaggatgat ttgttacgaa atttgatgat ccactgtaaa gccttgttat
    31861 ttagaaagac ttacggtatt ttttctaaaa atcctctaac aaaacaaatt cgatccaagt
    31921 atggagaatt atttttagtc actagaaaat ctgcggaaat tttagaagga gcatggttta
    31981 ttcggctaac agacgatgat attgcctatt tgacgatcca tattggagga tttttaaagt
    32041 atacaccatc gtctcaaaaa aatatgaaaa aagtttatct cgtttgtgat gaaggtgttg
    32101 cggtttcgag acttttgctg aaacaatgca aactttattt tccaaatgag caaattgaca
    32161 ctgtatttac aacagaacaa tttaagagtg tggaagatat tgcacaagtt gatgtagtga
    32221 ttactactaa tgatgatttg gatagcagat ttccgatttt aagggttaat cctatccttg
    32281 aagcagaaga tattttgaaa atgctagact atcttaaaca caatatattt cgtaatgaga
    32341 gcaaaagttt cagtgaaaat ctttctagtc ttatttcgtc ttatattgta gatagcaagt
    32401 tggctagtaa gttccaagaa gaggttcaaa cacttataaa tcaagaaata gtagttcaag
    32461 cttttttgga agatatttga aggacagtcc aatgatgaac acaaacctgt gtttttcttg
    32521 gtctttttta gtgttttgaa gggtggtata ctaatctcaa agataacaat tatatccaaa
    32581 ggaggcaaca tatgccaaac gtcaaagaaa ttacaagaga gtcatggatt ttagccactt
    32641 tcccagagtg gggaacatgg ttgaacgaag aaatcgaaga agaagtcgta cctgaaggca
    32701 actttgccat gtggtggcta ggcaactgtg gtacttggat taagacacca gctggtgcta
    32761 acgttgtcat ggacctttgg tcaaaccgtg gaaaatcaac caaaaaagtg aaagatatgg
    32821 ttcgtgggca ccaaatggca aatatggcag gtgttcgtaa gttgcaacca aacttgcgtg
    32881 ttcagccaat ggttatcgat ccatttgcta tcaacgaact agactattac ttagtttcac
    32941 acttccacag tgatcatatc gacccataca cagctgcagc aattctcaac aatcctaagt
    33001 tagagcatgt taagtttatc ggtccttacc actgtggacg aatctgggaa ggatggggtg
    33061 ttccaaaaga acgtatcatc gttgttaaac caggtgacac tatcgaatta aaagatatga
    33121 agattcatgc agtagaatca tttgaccgta cttgcttggt aactctccca gtgaacggtg
    33181 ctgatgagac aggcggtgaa cttgctggct tggctgttac agatgaagaa atggctcaaa
    33241 aggctgttaa ctatatcttt gaaacaccag gtggaaccat ctatcatggt gcagattctc
    33301 acttctcaaa ctattttgca aaacatggta aagactttaa aattgatgtt gctttgaata
    33361 actatggtga aaatccggta ggtatccaag acaaaatgac atctatcgac cttcttcgta
    33421 tggcagaaaa tctgcgtacc aaagtcatta tcccagttca ctatgatatc tggtctaact
    33481 tcatggcttc tactaatgag attctagaac tttggaaaat gcgaaaagat cgcttgcaat
    33541 acgatttcca tccatttatc tgggaagttg gcggtaagta cacttatcct caagatcaac
    33601 acttagtaga ataccatcat ccacgtggtt ttgatgattg ttttgaacaa gactctaaca
    33661 ttcaatttaa agctttgcta taatataaaa atattctttg gaggttatcc gtatgttgca
    33721 tgatacggat aattttcata taatagtaaa atagaatgtg tgattcaata atcacctcaa
    33781 atagaaagga aattctatgt caaatctatc tgttaatgca attcgttttc taggtattga
    33841 cgccattaat aaagccaact caggtcatcc aggtgtggtt atgggagcgg ctccgatggc
    33901 ttacagcctc tttacaaaac aacttcatat caatccagct caaccaaact ggattaaccg
    33961 cgaccgcttt attctttcag caggtcatgg ttcaatgctc ctttatgctc ttcttcacct
    34021 ttctggtttt gaagatgtca gcatggatga gattaagagt ttccgtcaat ggggttcaaa
    34081 aacaccaggt cacccagaat ttggtcatac ggcagggatt gatgctacga caggtcctct
    34141 agggcaaggg atttcaactg ctactggttt tgctcaagca gaacgtttct tggcagccaa
    34201 atataaccgt gaaggctaca atatctttga ccactatact tacgttatct gtggagacgg
    34261 agacttgatg gaaggtgtct caagcgaggc agcttcatac gcaggtttgc aaaaacttga
    34321 taagttggtt gttctttatg attcaaatga tatcaacttg gatggtgaga caaaggattc
    34381 ctttacagaa agtgttcgtg accgttacaa tgcctacggt tggcatactg ccttggttga
    34441 aaatggaaca gacttggaag ccatccatgc tgctatcgaa acagcaaaag cttcaggcaa
    34501 gccatctttg attgaagtga agacggttat tggatacggt tctccaaaca aacaaggaac
    34561 taatgctgta cacggcgccc ctcttggagc agatgaaact gcatcaactc gtcaagccct
    34621 cggttgggac tacgaaccat ttgaaattcc agaacaagta tatgctgatt tcaaagaaca
    34681 tgttgcagac cgtggcgcat cagcttatca agcttggact aaattagttg cagattataa
    34741 agaagctcat ccagaactgg ctgcagaagt agaagccatc atcgacggac gtgatccagt
    34801 cgaagtgact ccagcagact tcccagcttt agaaaatggt ttttctcaag caactcgtaa
    34861 ttcaagtcaa gatgccttga atgttgtggc agctaaatta ccaaccttcc taggtggatc
    34921 agctgacctt gctcactcaa acatgactta tatcaagacg gatggacttc aagacgatac
    34981 caatcgcttg aatcgtaaca ttcagtttgg tgttcgtgaa tttgcaatgg gaacaatctt
    35041 gaacgggatg gcccttcatg gtggacttcg tgtatacggt ggaactttct tcgtcttctc
    35101 tgactatgtg aaggcagctg tccgcttgtc agccttacaa ggacttcctg tgacttatgt
    35161 ctttacccat gattcaatcg cagttgggga agatggtccg actcacgaac cagttgagca
    35221 tttagcaggt cttcgtgcta tgccaaatct aaatgttttc cgtccagcag atgcgcgtga
    35281 aacgcaagca gcttggtacc ttgcagtgac aagtgagaaa acaccaactg cccttgtctt
    35341 gacacgtcaa aacttgactg ttgaagatgg aacagacttc gacaaggttg ctaaaggtgc
    35401 ttatgttgta tatgaaaatg cagccgactt tgataccatc ttgattgcga caggttcaga
    35461 ggttaatctt gctgtctcag ctgctaaaga attggctagt caaggcgaaa aaatccgcgt
    35521 agtcagcatg ccatctacag atgtctttga taaacaagat gcagcttaca aggaagagat
    35581 tcttccaaat gcagtccgcc gtcgtgttgc agtcgaaatg ggtgcaagtc aaaactggta
    35641 caaatatgtt ggtctcgatg gtgccgttct aggtattgat accttcggag cctctgcccc
    35701 agcaccaaaa gtattggcag aatatggctt tactgtagaa aatcttgtaa aagttgttcg
    35761 aaacttgaaa taatcctaaa aatcagggcg taagctctgg tttttcttac cagaaaagta
    35821 aggtacaatc ttgtaaaagt agctgaaatt tgatatagta gtcctatgta aaagacaaag
    35881 gagaaaacaa tgaatccaaa tattactttt ttaatcatgc ttgtaggtat gatggccttg
    35941 atgttcttta tgcaacgctc tcaaaagaaa caagctcaaa agcgtatgga aagcttaaat
    36001 aaactacaaa aaggctatga agtgattaca atcggtggac tttatggaac agtcgatgaa
    36061 gtagatacgg agaagggaac aatagttctt gacgtagatg gagtttactt gacttttgaa
    36121 ctagctgcta tcaagacagt attaccgctg aaagaaacag cttcactaga aggcgcaatt
    36181 gaaaaataag acgggatcac gaactcccgt tttctataaa agaaaggaaa tgggatgaaa
    36241 aaattagtct ttgtctgtct gggaaatatt tgccgtagcc ctatggccga gtttgttatg
    36301 aaatcaatga cagataacta cgaaatccaa agtcgagcaa cttcctcttg ggaacatggc
    36361 aatccgattc ataaggggac tcagggaatt tttcaagagt atgagattcc ttatgacaag
    36421 aacaagacat cgcttcagat tagtaaggaa gattttgaag cctttgatta tattatcgga
    36481 atggacgctt caaatatttc tgacttacgt cagatgtgtc cagtagactg tcaagataag
    36541 atttactcat tttcatctga aagtgttcca gacccttggt atacaggaga ttttgaagaa
    36601 acctatcgac gtgttcaaga gggctgtcaa gcttggttag aacgtttaga aaaggagagt
    36661 taaggtggaa aatcttacga atttttacga aaagtatcgt gtctatctga ctcgtccacg
    36721 tttagagctt ttggcagtag ttaccattgt tttctgtgct gtactcgtct tttttctaaa
    36781 tattccagga aaaggtgtct taaaactcga taatggaacg attgtttatg atggcagtct
    36841 tgtccgtggt aaaatgaatg gtcaaggtac cattaccttc caaaatggag accaatatac
    36901 aggtggcttc aacaatggag ccttcaacgg aaaaggtacc tttcaatcta aagaaggctg
    36961 gacctacgaa ggtgattttg taaatggtca ggctgaagga aaagggaaac taacaacaga
    37021 acaagaagtc gtttatgaag gaacttttaa acaaggcgtt tttcaacaaa aataaagcct
    37081 ccttatcaaa ggaggtatta ttagaattac aaggtaagcg cttacctgta aatccctttc
    37141 tttccaaatc cctcttccaa gcaagtttgt gaaataaaaa atatttgaaa taaatttcac
    37201 aaacttcaaa gataaaacct gataagaaaa gaaaatgaga aaagtttcac aagagtttaa
    37261 aaattctttg tgaaatgttt atgaaactgt ttacaaagtt gaaataatac gttataataa
    37321 acttgtgaaa aaattaacaa aggatatcgt tccttgaaag ctatggagga aaatatggct
    37381 gataaaaaaa ctgtgacacc agaggaaaag aaactcgttg ctgaaaaaca cgtagatgag
    37441 ttggttcaaa aagctctagt tgcccttgaa gaaatgcgta aattggatca agagcaagtt
    37501 gactacatcg ttgccaaagc atcagtagca gctttggatg cccacggaga attggcttta
    37561 catgcctttg aagaaacagg acgtggtgta tttgaagata aagcaactaa gaacttgttt
    37621 gcctgtgaac acgtagtaaa caacatgcgt cacactaaga cagttggcgt tatcgaagaa
    37681 gacgatgtaa caggattgac tcttattgct gaaccagttg gtgttgtttg tggtattact
    37741 ccaacaacaa acccaacatc aacagcaatc ttcaaatcat tgatttcatt gaagacacgt
    37801 aacccaatcg tctttgcctt ccatccatca gcacaagaat catctgctca tgcagctcgt
    37861 atcgtccgcg atgcagctat cgcagctggt gctcctgaaa actgtgtgca atggattact
    37921 caaccatcta tggaagcaac aagtgccctt atgaaccacg aaggtgttgc gacaatcctt
    37981 gcaacaggtg gtaatgccat ggttaaggcg gcttattcat gtggtaaacc agctcttggg
    38041 gtaggtgccg gaaacgttcc agcttatgtt gaaaaatcag caaacattcg tcaagcagca
    38101 cacgatatcg tcatgtctaa atcatttgat aacggtatgg tctgtgcatc tgaacaagca
    38161 gttatcattg ataaagaaat ttacgatgaa tttgtagcag agttcaaatc ttaccacact
    38221 tactttgtaa acaaaaaaga aaaagctctt cttgaagagt tctgcttcgg cgtcaaagca
    38281 aacagcaaaa actgtgctgg tgcaaaattg aacgctgaca tcgttggtaa accagcaact
    38341 tggattgcag aacaagcagg atttacagtt ccagaaggaa caaacattct tgctgcagaa
    38401 tgtaaagaag ttggcgaaaa tgagccattg actcgtgaaa aattgtcacc agttattgca
    38461 gttttgaaat ctgaaagccg tgaagatggt attactaagg ctcgtcaaat ggttgaattt
    38521 aacggtcttg gacactcagc agctatccac acagctgacg aagaattgat taaagaattt
    38581 ggtaaagctg ttaaagctat tcgtgttatc tgtaactcac cttctacttt tggtggtatc
    38641 ggggacgttt acaatgcctt cttgccatca ttgacacttg gatgtggttc ttacggacgc
    38701 aactcagttg gggataacgt tagtgccatt aacctcttga atatcaaaaa agtcggaaga
    38761 cggagaaata acatgcaatg gatgaaactt ccttcaaaaa catactttga acgtgattca
    38821 attcaatacc ttcaaaaatg tcgtgacgtt gaacgtgtca tgatcgttac tgaccatgcc
    38881 atggtagagc ttggtttcct tgatcgtatc atcgaacaac tggaccttcg tcgcaataag
    38941 gttgtttacc aaatctttgc ggatgtagaa ccggatccag atatcacaac tgtaaaccgt
    39001 ggtactgaga ttatgcgtgc cttcaaacca gataccatca tcgcactcgg tggtgggtct
    39061 ccaatggatg ctgccaaagt aatgtggctc ttctacgagc aaccagaagt ggacttccgt
    39121 gaccttgtcc aaaaattcat ggatatccgt aaacgtgcct tcaagttccc attgcttggt
    39181 aagaagacta aattcatcgc gattccaact acatctggta caggatctga agtaacacca
    39241 tttgccgtta tctctgataa agcaaacaat cgtaaatacc caatcgctga ctactcattg
    39301 acaccaactg tggcaatcgt agatcctgct ttggtattga cagttccagg atttgttgct
    39361 gctgatactg gtatggacgt attgactcac gcgacagaag catacgtatc acaaatggct
    39421 agtgactaca ctgatggttt agcacttcaa gccattaaat tggtctttga aaatctcgaa
    39481 agctcagtta agaatgcaga cttccactca cgtgagaaaa tgcataacgc ttcaacaatc
    39541 gctggtatgg cctttgccaa tgccttccta ggtatttctc actcaatggc ccataagatt
    39601 ggtgcgcaat tccacacaat ccacggtcgt acaaatgcta tcttgcttcc atacgttatc
    39661 cgttacaacg gtacacgtcc agctaagaca gcaacatggc ctaagtacaa ctactaccgt
    39721 gcagatgaaa aataccaaga tatcgcacgc atgcttggac ttccagcttc tactccagaa
    39781 gaaggggttg aatcttacgc aaaagctgtc tacgaactcg gtgaacgtat tgggatccaa
    39841 atgaatttta gagaccaagg aattgacgaa aaagaatgga aagaacattc tcgtgaatta
    39901 gccttcctgg cttatgaaga ccaatgttca ccagctaacc cacgtcttcc aatggtagac
    39961 catatgcaag aaatcatcga agatgcatac tatggctaca aagaaagacc aggacgccgt
    40021 aaataattgt ttatcagtct agaagcaaga caaaaactca atttgaggga aagatccagt
    40081 aatttttcta tgataaaagg catcctatca aggtttttga acacctgata ggatgccttt
    40141 ttatgatatt gaggcctttt tgcccttttt gaaaaactag aatagaaaca aaatatataa
    40201 tagattgaaa ctagagtagt acacatctgc ttctaaaaca ttgttagaat tcgatttgac
    40261 tgtcctgatc aatttgtcct gttcttattt cattttaaca tataaaaaat at
//
LOCUS       DAVLVZ010000014        39760 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000014 DAVLVZ010000000
VERSION     DAVLVZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.014"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            29..373
                     /locus_tag="VYN77_000674"
     CDS             29..373
                     /locus_tag="VYN77_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558162.1"
                     /translation="MPLPTGKIDLNEYGKHLAEKSAEIYKEKTKQAVDAMERHRQECL
                     ARDAKWQAVIDRSDELLKEKKEREHQQEIERLMEEKRKEVDREVSQRLSMPNEHQKKL
                     NKAMSEMLKDLF"
     gene            571..1869
                     /locus_tag="VYN77_000675"
     CDS             571..1869
                     /locus_tag="VYN77_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558163.1"
                     /translation="MTLKGMGYLRKKLANYKMGVDTRYNQYAMQHNDIDVGITIPPQI
                     RQQYRAVLGWTAKGVDSLADRLVFREFANDEFGANEIFAQNNPDVFFDSAILSALIGS
                     CCFVYISQGDDDDAPRLQVIEASNATGVLDPITGLLIEGYAVLKRDDNGYAVLEAYFT
                     SDVTWFYPKDGKPFAIENPTGVPLLVPVIHRPDTVRPFGRSRITKAGMYYQRYAKRTL
                     MRSDVTAEFYSFPQKYVLGLSQDAEAIDTWKATVSSLLTFTKDDEGEKPNVGQFTTSS
                     MSPFTEQLRTAAAGFSGEMGLTLDDLGFVSDNPSSVEAIKASHENLRLAGRKAQRSLG
                     SGLLNVAYVATCLCDEFPYLRKQFNKTVVKWEPLFEADANMLTLIGDGVIKLNQAVPG
                     YMDAETIRDLTGIKGSDKPAPVVKEGADGGRYVSESTPEN"
     gene            1878..2135
                     /locus_tag="VYN77_000676"
     CDS             1878..2135
                     /locus_tag="VYN77_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558164.1"
                     /translation="MKKARLDSEILEELLEKNKVNYLDANQYAIEIGEILAKVLGAYL
                     SNINQEDVERRKQIGIESAEERKRKRDERIAKFGNPRRRLN"
     gene            2172..2564
                     /locus_tag="VYN77_000677"
     CDS             2172..2564
                     /locus_tag="VYN77_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1558165.1"
                     /translation="MELDKFKTMMNVRKRMTYFLRFQRMAGSENQVRIDEEAWKLVLP
                     DQWHLSGEHEKAIREGLEIFAHDINSIENERARKYFIIHYCYMRKKTMSECVEMAGTS
                     STSYHRYKQISVLNFARIHQNGELEAYK"
     gene            complement(3520..4368)
                     /locus_tag="VYN77_000678"
     CDS             complement(3520..4368)
                     /locus_tag="VYN77_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1558166.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLACLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            4520..4798
                     /locus_tag="VYN77_000679"
     CDS             4520..4798
                     /locus_tag="VYN77_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558167.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            4800..6863
                     /locus_tag="VYN77_000680"
     CDS             4800..6863
                     /locus_tag="VYN77_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1558168.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(6941..8197)
                     /locus_tag="VYN77_000681"
     CDS             complement(6941..8197)
                     /locus_tag="VYN77_000681"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1558169.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            8339..10804
                     /locus_tag="VYN77_000682"
     CDS             8339..10804
                     /locus_tag="VYN77_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1558170.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLCFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLATADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            10869..11780
                     /locus_tag="VYN77_000683"
     CDS             10869..11780
                     /locus_tag="VYN77_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1558171.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            12010..12708
                     /gene="dapD"
                     /locus_tag="VYN77_000684"
     CDS             12010..12708
                     /gene="dapD"
                     /locus_tag="VYN77_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1558172.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            12776..13906
                     /locus_tag="VYN77_000685"
     CDS             12776..13906
                     /locus_tag="VYN77_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1558173.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            13918..14457
                     /locus_tag="VYN77_000686"
     CDS             13918..14457
                     /locus_tag="VYN77_000686"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1558174.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVAYDPQQLVKTSFALLEPQ
                     GDVEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            14441..15118
                     /locus_tag="VYN77_000687"
     CDS             14441..15118
                     /locus_tag="VYN77_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1558175.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(15185..16084)
                     /gene="galU"
                     /locus_tag="VYN77_000688"
     CDS             complement(15185..16084)
                     /gene="galU"
                     /locus_tag="VYN77_000688"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1558176.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYKRTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(16106..17122)
                     /locus_tag="VYN77_000689"
     CDS             complement(16106..17122)
                     /locus_tag="VYN77_000689"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1558177.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            17334..17774
                     /locus_tag="VYN77_000690"
     CDS             17334..17774
                     /locus_tag="VYN77_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1558178.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVFDK
                     IFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEILSFW"
     gene            complement(17897..18313)
                     /locus_tag="VYN77_000691"
     CDS             complement(17897..18313)
                     /locus_tag="VYN77_000691"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1558179.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(18381..19031)
                     /gene="phoU"
                     /locus_tag="VYN77_000692"
     CDS             complement(18381..19031)
                     /gene="phoU"
                     /locus_tag="VYN77_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1558180.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(19046..19798)
                     /gene="pstB"
                     /locus_tag="VYN77_000693"
     CDS             complement(19046..19798)
                     /gene="pstB"
                     /locus_tag="VYN77_000693"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1558181.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(19800..20615)
                     /gene="pstA"
                     /locus_tag="VYN77_000694"
     CDS             complement(19800..20615)
                     /gene="pstA"
                     /locus_tag="VYN77_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1558182.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(20608..21471)
                     /gene="pstC"
                     /locus_tag="VYN77_000695"
     CDS             complement(20608..21471)
                     /gene="pstC"
                     /locus_tag="VYN77_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1558183.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(21589..22464)
                     /locus_tag="VYN77_000696"
     CDS             complement(21589..22464)
                     /locus_tag="VYN77_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV1558184.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(22631..23962)
                     /locus_tag="VYN77_000697"
     CDS             complement(22631..23962)
                     /locus_tag="VYN77_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1558185.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(23963..24670)
                     /locus_tag="VYN77_000698"
     CDS             complement(23963..24670)
                     /locus_tag="VYN77_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1558186.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(24754..25230)
                     /locus_tag="VYN77_000699"
     CDS             complement(24754..25230)
                     /locus_tag="VYN77_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1558187.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKSTIWGSTVDKDDALVSCLVAPGFEFEDFELFERADLLATYPEHKEMIEHLTRY"
     gene            25237..25764
                     /locus_tag="VYN77_000700"
                     /pseudo
     CDS             25237..25764
                     /locus_tag="VYN77_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26079..27770)
                     /locus_tag="VYN77_000701"
     CDS             complement(26079..27770)
                     /locus_tag="VYN77_000701"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1558188.1"
                     /translation="MNTKELIASELASVIDSLDQEAILNLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKINSQSFEKVVATGPYVNFFLDKSAISAQVLQAVITEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGNEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFSRLYNELQVEFDSYNGEAFYNDKMDAIVDILSEKGLL
                     VESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAISR
                     AKAQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQAFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            27906..28352
                     /gene="argR"
                     /locus_tag="VYN77_000702"
     CDS             27906..28352
                     /gene="argR"
                     /locus_tag="VYN77_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1558189.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVFVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLANETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDANKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            28403..30937
                     /gene="mutS"
                     /locus_tag="VYN77_000703"
     CDS             28403..30937
                     /gene="mutS"
                     /locus_tag="VYN77_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1558190.1"
                     /translation="MATEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVAIDRDGSQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKEGFEDLHLLDSRLASVEQA
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIIQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDGIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRRVLREGTGWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            31083..32777
                     /gene="patA"
                     /locus_tag="VYN77_000704"
     CDS             31083..32777
                     /gene="patA"
                     /locus_tag="VYN77_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1558191.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(33107..33370)
                     /locus_tag="VYN77_000705"
                     /pseudo
     CDS             complement(33107..33370)
                     /locus_tag="VYN77_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            33568..35334
                     /gene="patB"
                     /locus_tag="VYN77_000706"
     CDS             33568..35334
                     /gene="patB"
                     /locus_tag="VYN77_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1558192.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEIRHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            35473..36162
                     /locus_tag="VYN77_000707"
     CDS             35473..36162
                     /locus_tag="VYN77_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1558193.1"
                     /translation="MARTVVGVTANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            36166..36345
                     /locus_tag="VYN77_000708"
     CDS             36166..36345
                     /locus_tag="VYN77_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558194.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            36332..37681
                     /locus_tag="VYN77_000709"
     CDS             36332..37681
                     /locus_tag="VYN77_000709"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1558195.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            37805..39265
                     /locus_tag="VYN77_000710"
     CDS             37805..39265
                     /locus_tag="VYN77_000710"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1558196.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            39279..39713
                     /locus_tag="VYN77_000711"
     CDS             39279..39713
                     /locus_tag="VYN77_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1558197.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACRKFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT        11640 a         7537 c         8825 g        11758 t
ORIGIN      
        1 taacaaatag aaagatgagg tatatagaat gcccctacct actggtaaga ttgatttaaa
       61 cgaatacggg aagcatttag cagaaaagtc agctgagatt tacaaagaga aaactaaaca
      121 agcagttgac gcgatggagc gacatagaca agaatgttta gcaagagacg ccaagtggca
      181 agctgtcata gatcgttccg atgaactatt aaaagaaaag aaggaaagag agcaccagca
      241 agagattgaa cgcttgatgg aagagaagcg caaagaggtt gatagagaag ttagtcagcg
      301 cctatcaatg ccaaatgaac accagaaaaa actgaacaaa gcgatgagtg aaatgttgaa
      361 agatttattt taaagagaac ataaaaagac tccataaaaa aataattaaa aagtttttta
      421 ataaagtgaa tcggaaaaat attttttgtg aaagagattg gagaagcaag ggagaaggag
      481 gggggatacc cccctccccc tcggagctcc cgaacttccc accatcactg tacatttttt
      541 ctcgcgggaa atgaaaggta gttgtataaa atgacattga aaggtatggg ctatctcagg
      601 aagaagctag ccaattataa aatgggtgta gatactagat acaatcagta tgctatgcaa
      661 cacaatgaca tagatgttgg tattacgata ccacctcaaa tcaggcaaca atatcgagcg
      721 gtcttaggtt ggactgctaa gggtgttgac agtcttgcag atcgtttggt ctttcgtgag
      781 tttgccaatg atgagtttgg agcgaatgaa atctttgctc agaacaatcc agatgtattc
      841 tttgatagcg cgattctttc ggctctgatt gggtcgtgtt gttttgttta catctcgcaa
      901 ggggacgatg atgacgctcc tcggttgcag gttatcgagg cgagtaatgc aactggtgtt
      961 ctggatccta tcactggctt gttgatagag ggctatgccg ttttgaaaag ggatgacaat
     1021 ggttatgccg tgcttgaggc ttattttact agtgatgtga cttggttcta tccgaaagat
     1081 ggtaagccgt ttgcaatcga aaatccgacg ggtgttcctt tgctggtgcc agtcattcat
     1141 aggcctgata ctgttcggcc gtttggtcgg tcacgaatta ctaaggctgg gatgtactat
     1201 cagagatatg ctaaacgaac gcttatgcgg tcagatgtta ctgctgagtt ctattcattc
     1261 cctcagaagt atgtgttggg attgagtcaa gatgctgagg cgattgatac ttggaaagca
     1321 actgtatcta gcttgctgac gtttacgaaa gatgatgagg gggaaaagcc gaatgtggga
     1381 caattcacca cgtccagcat gtctcctttt actgagcagt tacggactgc agcagctgga
     1441 ttttctgggg aaatgggatt gaccttggat gatcttggtt ttgtttctga caatccgtca
     1501 tccgttgaag ccatcaaggc tagtcatgag aacttgcgtt tagctggtcg gaaggctcag
     1561 cgctctctgg gttctggtct gctgaatgtg gcttatgtcg ctacttgttt atgtgatgag
     1621 tttccttatt tgaggaaaca gttcaataaa acggtcgtga agtgggagcc tttatttgag
     1681 gcggacgcta acatgctgac cttgattggt gatggtgtta tcaaactgaa tcaagcggtg
     1741 cctggctata tggatgctga aaccatccgt gacttgactg gaattaaggg gtcagacaag
     1801 cctgctccag tagtgaagga gggtgcagat ggtggaagat atgtttccga gtctactcca
     1861 gaaaattaag gacgaatttg aaaaaagcta ggcttgatag tgagattctg gaagagttac
     1921 tagaaaagaa taaagttaat tatctggacg ccaatcaata tgctatagaa attggtgaaa
     1981 tactcgcaaa ggtcttagga gcttacttga gtaatatcaa tcaagaagat gtggagcgtc
     2041 gaaaacagat aggtatagag tctgctgaag aacgtaaaag aaaacgagat gaaaggattg
     2101 ccaagtttgg aaatcctaga agaagattaa actaggcata agttacagga gtagaaaggt
     2161 tgtcgttaaa aatggagctg gataagttta aaacgatgat gaacgtcaga aagcggatga
     2221 cttactttct gagatttcag aggatggcag gtagtgaaaa ccaagttagg atagatgaag
     2281 aggcttggaa acttgtctta cctgatcagt ggcatttgag cggtgagcat gaaaaagcaa
     2341 tccgtgaggg gttggagata ttcgcccacg acatcaacag catagagaac gagagagcca
     2401 gaaaatactt cattatccat tattgctata tgagaaagaa aactatgagc gaatgcgtag
     2461 aaatggcagg tactagctcc actagctacc accgatacaa acagatatcc gtcttaaact
     2521 ttgcaagaat ccaccagaac ggagagctag aagcatataa gtaggctgag gctgcatatt
     2581 cgccccctta tttcgctttg tttcgtactg gacaactaaa acaccataga taaactagaa
     2641 gcccttagaa acgaatctgg ggggcttttg tgaggtcata atcttataaa ctacgcgctt
     2701 catggtataa tatacctatc agcaatcagc aataaaaaag cacgtttgac cgtgctagtt
     2761 tcttgcctgc tgaactcgtc atttattgcc cttttgtggg gctttttttt gtggactttt
     2821 tgagaatttt taagaacttt taatgatttc taatcaaatg agatttttca aaaagtcagt
     2881 aatatcaagg gtttaggcgc tatattgagg tgtgaaatga tgcctaagtc aaattattat
     2941 atactgttat tatagtcaag aaaaatatgc tattatgtta aaaaaatatt tttcaaaata
     3001 taaatggacg gatttatttt ggattttatt tgttattttg acctgcctct atattggtaa
     3061 ccatgatttg tttactctca atcatcaaga attctctttt cgtggtagcg tttggggtct
     3121 ggtactggcc ttatatcact tattgttcat tgataagttt gtaatttcga atcgaaaata
     3181 aagattagag ctatgcttga ctgtgtactt ttaggattga ttttggagga agattttgtc
     3241 tctattattt attattttaa atttatttat tttgtataag atctattctt tgaggcggga
     3301 aaaatcgaaa tacttgattt atacggccta tatcatattt ggggtaaatg tagtatatgg
     3361 tattcaatgg ttattaagag aactgatttc aactatttcc ccttaatgtg tatggaaact
     3421 ataacaaaac gcataagatt aaggtttgtg tacctgttct tatgcgtttt tgtcatattg
     3481 aagattttcg gtaagtttta tacctttggg aagtctagtt tagaatgctt tcccaaccag
     3541 aatctctgct tcgatggtaa tctctgtcag ttggctccag tcaatcttgc tctggtcaac
     3601 atgaaagagg agaggagtca tactgagtag ggcttggagt tgttctgctg tgatagtctt
     3661 agtcagagag gcagtttgac tagataggat ggtaaagtgt tcctggaaat gctccttgat
     3721 atcttggtta gaatactcct tgtttgtcag ctggtcctgt actctttgac ggatttcttt
     3781 gaggtgattt tcagttggga taacctttat caagataccg tctttggata aaacgcgacg
     3841 aaattctcca tagttggcag gtgagaagat atcaagcaga atatccatgt tagcgtcttt
     3901 tataggaagg catgccaagt cgccaacgaa ccaattgact gcccagttgg gttcactttt
     3961 agcagcgatt tggactgaat ctttggagat gtcaaaggca tagaaagttt tttcagagtg
     4021 actttcttgt agtttgcgag aatagaatcc ttcaccacaa ccgatatcca aaattgttgt
     4081 ggtagttttt gagcttgcaa gcaagtcaga tacagcatct aagatagctt ggtaaaagcc
     4141 ggcttctagg atttgttgac ggttttgaaa attttccttg tcatagttag cagattgctt
     4201 gatttgaggt gctagattga catagccaaa tttcgccaag tcaaaagaat gacggttgca
     4261 gcacttgaaa ttagtctcta acagagtcag attttcttga cagataggac aggcaaaggc
     4321 agtcgcagaa gcaaaacgct gaagtttggg cttgagattt gtattcatag tttaagtgta
     4381 tcaaaagagg caaatgaaag caactttagg aataagtaga tgggagattc agtagaaata
     4441 aaactaattc tccaatttat agaatgaaat tgcttttata tctaaattgc tatataataa
     4501 tgataaggag gaaataagta tgttaaaaga agtattaacc gttgcaaaag ttgcgaaaaa
     4561 atcttcatta tttttgggtg gtgtcgcatt tggtaccctt ggtttgaaaa tcttagcaag
     4621 taaggaagct aaaaaaggtt attctaaagc tttggctaag gcttacaagt tgaaagacga
     4681 gctagatgca tctgtttctg ttgtgaagca acatggagac gatgtcttgc aagatgccaa
     4741 atatttgtac gagcaagaga aaaaagaaga gcaattagat agccttatag gtgaataata
     4801 tgtcttttaa agtgctacat agaggatacc aacatatccg actatcatct tctttttcac
     4861 ttaccttgga tattcaagac tatcttcgtt ccttggcgag agatgaaaag gggattgagt
     4921 ctatccagtt ttacatggat caacagcact ttactctacg cataaaagaa ggcttttctg
     4981 tattagataa tgcagaagcc tttttaaaaa gaattgataa agggaaagtt tctgagttga
     5041 tgactcttcc cattcgtaga gaagagagtg cttattctat tgtttcaggt gcagcggtta
     5101 agcgtgtact ttttcgtagt tttgtgccgt atcctattcg ctatatatgg acttgttatc
     5161 aggctttggg ttatattaga gaagcctatc aaacactagc gcgtaaggaa ctaacgatgg
     5221 aagtcttgga ctgttcggct attttattgt ccttgtttat gaaccaatcc aagacagcta
     5281 gcaatatcat gtttatgctt gatttgggga atcatttaga tcagtggtcc ttgaaaaaaa
     5341 ctgcaacaga tttagaacag agtcttcttg caaaagagag cgatgtattc ctagtacagg
     5401 gcgatacggt tgttagtata aagagttccg atgttcaaat aggagatgtc ttgatcttat
     5461 ctcaaggaaa tgaaattctg tttgatgggc aagtagtttc aggtttaggt atggtcaacg
     5521 aaagttcctt gacaggagag agttttccag ttgaaaaaag agagtctgat ttggtttgtg
     5581 caaatacagt attagaaact ggagagttac gcattcgtgt aacagataat cagatgaaca
     5641 gccgtatttt acagctgatt gagttgatga agaaatctga agaaaacaag aaaacgaaac
     5701 aacgctattt catcaagatg gcggataaag tcgtcaaata taatttcttg ggggctgggc
     5761 tgacttacct attgacaggt tctttttcta aggctatttc tttcctattg gtcgatttct
     5821 cctgcgcttt gaaaatctct actcctgtag cttatttgac agttatcaag gaggggttga
     5881 accgtgaaat ggtgattaag gatggagatg ttctggagaa atatctggaa gttgatactt
     5941 tcttgtttga taagacagga acaatcacaa ctagttatcc tatagttgaa aaggtgttac
     6001 cttttggaga ctatagtgag gaagatattc tcagaatcag tgcctgtctt gaggaacaca
     6061 tttatcatcc tattgctaat gccatcgtca agcaagctga gatagaggga attgaacatg
     6121 aggaaatgca tgggaaactc caatatatcg caagcaaggg gatcaaatct catatagatg
     6181 gccaaccagt tcttattggg aattatgtct tgatgcagga tgagcagatt catatcagtt
     6241 cagaacaaaa tgctttaatt gaagagtaca agagtcacta caatctctta ttcttggctt
     6301 atcaaaatga attgattgga atgttctgca ttcatactcc tttgagaaaa gaagcaaaaa
     6361 cagccttgga taaacttaag gcacagggga aaaaattgat tctggcaaca ggagacacct
     6421 tgattaggac agaggaatta gtcaaagatt tgccctttga tcaggtctat acagacttga
     6481 aacctgatgg gaaatttgag ttagtagaga aactgcagaa agcaggtcac actattttga
     6541 tggttggaga tggcttgaat gactcagcgg ctttaactct atcagatatc ggtgtggtga
     6601 tgaatgagag tgcagatatt tctaagcaga tgagtgatat cttattgtta gataatcgtt
     6661 tggatttctt ccaagagttg gattcgttat catcatcttt gcaaacactc atcaagaaaa
     6721 atattcagga taccgttgtc gtaaatagta gtttgattgg ctttggctta tttaattggt
     6781 tcagtccttc aaatctctct atcttacata atctaacaac cttgcgcatt gtcctgcgta
     6841 gcctgtctat taaaaataga tagatgagag ttattaacag caaaaaggag ttacctttct
     6901 cgaggttaac tcctttgttt atagataaat gttgaatagt ttagtaagtc aatacaaagt
     6961 attttttctt cccacgacgg ataacagtca gttcattttc taacttatca gcgtcactca
     7021 agacatagtc aaggtcttgg atgcggtcgc cgtttacgta gatggctccg ttttggacgt
     7081 cttcacgggc ttggcgtttt gagttaacta taccagatga gacgagcagt tccacgatat
     7141 tgttgttttc gtctgcctgt acttggtagt ttggcacacc acgaagtcct tgtttgagct
     7201 ctttgacaga aaggtttttg atgtttcctg caaagagttg ctcagtgatg ttaagtgctt
     7261 ctttgtaggc ttcttctccg tgaacaagtg taacaacttc acgagccaag actttttgag
     7321 ccaagcgttc gtgtggcgct gcttcaaatt gtttacgaat atcttcaatc tcatcaagtg
     7381 acaagaaagt aaagattttc aagaagcgaa cagcgtcagc gtccatcacg ttcatccaga
     7441 attggtacat ttcgtatgga gaagtctttt cgggattgag ccagacggca tttccttctg
     7501 atttaccaaa tttcttacca gttgcatctg tgattagtgg aacagtgata acgtgaccag
     7561 tcttgtccgc cttacgacga agcaattcgg taccagctgt catatttccc cactggtcag
     7621 aaccaccgat ttgaagagtg acattatggt cttggttaag gacgaagaag tcgtaccctt
     7681 gcatgatttg gtaagcgaac tcagtgtaag aaattcctgt ttcgatccgt tttttaacag
     7741 attccttact catcatgtag ttgaccgtga agtattttcc aatatcacgg aggaagtcaa
     7801 tgaagctgat gctgccaaac cagtcgtagt tgttgaccat gacagccttg ttttcgccat
     7861 tttcaaagtc aagaaaacga gaaagttgtc cttggataga cttgacccag ccatctactg
     7921 tgtcttttgt ttggagacta cgttcagcat ctttgaagga cggatctccg atgagacctg
     7981 tagcaccgcc aacgagcgca taaggtttgt gacctgctag ttgcaagcga cgacttgtca
     8041 agattgcgac aaggtggcct aggtgaaggc tgtcagcagt tggatcgtag ccagtatagt
     8101 aagaaacttg accttcttct agggctttac gcaaagcttc ttcatcagtc gtttgaaata
     8161 tcaaaccacg ctcttttagc tcatcaaaaa tgtgcatgtg tcttttctcc tttttaaaat
     8221 tattgtttct acctattgta tcacaaactt gggcaatagc ctagcagaat agggaagaaa
     8281 gtggctattt tattgaaaat ttcccttttg tggtataata gataaagtga ggaaatccat
     8341 gcaaaatcaa ttaaatgaat taaaacgaaa aatgctggaa tttttccagc aaaaacaaaa
     8401 aaataaaaaa tcagctagac ctggcaagaa aggttcaagt accaaaaaat ctaaaacctt
     8461 agataagtca gccattttcc cagctatttt actgagtata aaagccttat ttaacttact
     8521 ctttgtactc tgttttctag gaggaatgtt gggagttggg attgctttgg ggtacggagt
     8581 ggccttattt gacaaggttc gggtgcctca gacagaagaa ttggtgaatc aggtcaagga
     8641 catctcttct atttcagaga ttacctattc ggacgggacg gtgattgctt ccatagagag
     8701 tgatttgttg cgcacttcta tctcatctga gcaaatttcg gaaaatctga agaaggctat
     8761 cattgcgaca gaagatgaac actttaaaga acataagggt gtagtaccca aggcggtgat
     8821 tcgtgcgacc ttggggaaat ttgtaggttt gggttcctct agtgggggtt caaccttgac
     8881 ccagcaacta attaaacagc aggtggttgg ggatgcgccg accttggctc gtaaggcggc
     8941 agagattgtg gatgctcttg ccttggaacg cgccatgaat aaagatgaga ttttaacgac
     9001 ctatctcaat gtggctccct ttggccgaaa taataaggga cagaatattg caggggctcg
     9061 gcaagcagct gagggaattt tcggtgtaga tgccagtcag ttgactgttc ctcaagcagc
     9121 atttttagca ggacttccac agagtcccat tacttactct ccttatgaaa atactgggga
     9181 gttgaagagt gatgaagacc tagaaattgg cttaagacgg gctaaggcag ttctttacag
     9241 tatgtatcgt acaggtgcat taagcaaaga cgagtattct cagtacaagg attatgacct
     9301 taaacaggac tttttaccat cgggcacggt tacaggaatt tcacgagact atttatactt
     9361 tacaactttg gcagaagctc aagaacgtat gtatgactat ctagctcaga gagacaatgt
     9421 ctccgctaag gagttgaaaa atgaggcaac tcagaagttt tatcgagatt tggcagccaa
     9481 ggaaattgaa aatggtggtt ataagattac tactaccata gatcagaaaa ttcattctgc
     9541 catgcaaagt gcggttgctg attatggcta tcttttagac gatggaacag gtcgtgtaga
     9601 agtagggaat gtcttgatgg acaaccaaac aggtgctatt ctaggctttg taggtggtcg
     9661 taattatcaa gaaaatcaaa ataatcatgc ctttgatacc aaacgttcgc cagcttctac
     9721 taccaagccc ttgctggcct acggtattgc tattgaccag ggcttgatgg gaagtgaaac
     9781 gattctatct aactatccaa caaactttgc taatggcaat ccgattatgt atgctaatag
     9841 caagggaaca ggaatgatga ccttgggaga agctctgaac tattcatgga atatccctgc
     9901 ttactggacc tatcgtatgc tccgtgaaaa tggtgttgat gtcaagggtt atatggaaaa
     9961 gatgggttac gagattcctg agtacggtat tgagagcttg ccaatgggtg gtggtattga
    10021 agtcacagtt gcccagcata ccaatggcta tcagacctta gctaataatg gagtttatca
    10081 tcagaagcat gtgatttcaa agattgaagc agcagatggt agagtggtgt atgagtatca
    10141 ggataaaccg gttcaagtct attcaaaagc tactgcgacg attatgcagg gattgctacg
    10201 agaagttcta tcttctcgtg tgacaacaac cttcaagtct aacctgactt ctttaaatcc
    10261 tactctggct actgcagatt ggattgggaa gactggtaca accaaccaag acgaaaatat
    10321 gtggctcatg ctttcgacac ctagattaac cctaggtggc tggattgggc atgatgataa
    10381 tcattcattg tcacgtagag caggttattc taataactct aattacatgg ctcatctggt
    10441 aaatgcgatt cagcaagctt ccccaagcat ttgggggaac gagcgctttg ctttagatcc
    10501 tagtgtagtg aaatcggaag tcttgaaatc aacaggtcaa aaaccaggga aggtttctgt
    10561 tgaaggaaaa gaagtagagg tcacaggttc gactgttacc agctattggg ctaataagtc
    10621 aggagcgcca gcgacaagtt atcgctttgc tattggcgga agtgatgcgg attatcagaa
    10681 tgcttggtct agtattgtgg ggagtctacc aactccatcc agctccagca gttcaagtag
    10741 tagttctagc gatagcagta actcaagtac tacacgacct tcttcttcaa gggcgagacg
    10801 ataattctct aaatgaagtg gccaatcaat ggattgccac ttttttcttt tcgtgttaca
    10861 ataagggtat gaatcagtat cagaaaaaga ttgttaacgg aaaaatttat tcgctcctat
    10921 ccggcttaat atggggaatc tgtggaattt taggagagta cttctttact cattatcagg
    10981 tgtcttcggg ctggattacc tctatgcgtt tgacactggc agggagtctt gtactcattt
    11041 ggtctgcaat acaattaaaa tcgcaagtgc tagatatttg gcgagacaag aaaaattacc
    11101 tgcccttttt agcctatgct attttgggga ttttttcagt tcagtatttt ttctatctct
    11161 gtgtagaata ctcaaatgct acgacagcaa ctattttaca gtttattagc cctgtcttta
    11221 tcctctttta caatcgcttg gtttatcaaa aacgagcgtc aaaaagcgct gttttctatg
    11281 ttttggttgc catgctgggt gtttgcttga tggcgacaaa gggagacctc tctcagttat
    11341 ccatgacgcc gctagctctt ataacaggtt tgctgagtgc catgggtgtt atgtttaatg
    11401 ttatcttgcc ccaacctttt gctaagcgtt atggttttgt tcctacggtt gggtggggga
    11461 tgattttggc aggtttgttt agcaatgtcc tctcgccggt ttatcagctt tcctttactc
    11521 ttgatatttg gagtatcttg atttgcctca ttatcgcttt ctttggaacg gcttttgctt
    11581 ttttcatttc catgaaggct gtgtccttgg tttctccttt ggtggttgcc gttatcagtg
    11641 ccagtgaacc tctctcttct gctctcttga gtgttttgtt cttaggatta gtagtggatt
    11701 ggtccctcct tctagctata gccttgatta ttttacccat gatatttttg tctatagaag
    11761 aagcgaaaga aagtagataa aaagtctttt cttaattcta aaagtgtgct atactggaat
    11821 agtcaataaa acacggggag atagttgtga agagtgtttt taggttgtat cggtaggggc
    11881 ttgcttttac cgacagcacg ttttatctca catccctaaa aaatcatacc taaaaacaga
    11941 caaaaaggtt tcaaatttga ggcctttttt ggtagaatag gtatcattat aacgaaccag
    12001 gaggcaccta tgactgctac aaaaatgaac gctcaagaaa ttatccaatt tatcgccaat
    12061 gctgaaaaga aaaccagtgt caaagtaacc tttgaggggc aactcgcaac tgctgtgcct
    12121 agctctgttg tcaaactagg aaatgtccta ttcggagact ggaaggacgt ggctccgctt
    12181 cttgaaggtt tggtagaaaa tcaagattat gttgtcgagc aagatgctcg taattctgca
    12241 gttcctttgc tagataagcg tgctatcaac gctcgtatcg agccaggtgc tattatccgt
    12301 gaccaggtgg aaattggtga caatgctgtt atcatgatgg gagctgttat caatatcggt
    12361 gctgaaatcg gtgctggaac catgattgac atgggtgcca tccttggtgg ccgtgccatc
    12421 gttggaaaaa atagccacgt tggtgcaggt gcagttttgg caggtgtgat tgagccagct
    12481 agtgctgaac cagtccgtgt cggagacaat gttctcatcg gggctaatgc agtggttatc
    12541 gaaggagtcc aaatcggtag tggttcagtt gtcgcagcag gagctattgt tacccaagat
    12601 gtcccagaaa acgtggtagt agcaggtgtt ccagctcgta ttatcaaaga aattgatgcc
    12661 caaactcaac aaaaaacagc gctagaggat gcgcttcgta ccttgtaatt gtaaaagtaa
    12721 aaaagaggcg gaaccctttt tccagcctct ttctgctata tcggaggaca gatagatgtt
    12781 agatttgatt cagactagac gagatttaca ccagattcca gagattggct tggaggagtt
    12841 caagactcag gcttatttgc tggatgtgat tgagaaattg actacgggca aggattttgt
    12901 tcaaattcgt acttggcgga cagggatttt ggtctacctg cagggaagtc agccggagcg
    12961 aaccattggt tggcggacag atattgatgg cctgcctatc gtcgaacaaa caggtctacc
    13021 ttttgcttct caacaccaag gtcgcatgca tgcctgtggt catgattttc atatgaccat
    13081 tgccttaggc tgtcttgagc gcgcccttga ggagcaaccc aagaataatc tgctctttct
    13141 atttcagcct gctgaagaaa atgaagctgg tggtatgctc atgtatgagg acgatgcttt
    13201 tggagattgg ttgccagatc aattctatgg tctccatgtt cgtccagatt tgaaagtcgg
    13261 ccagattgcg actaatactc atacactctt tgcaggaact tgcgaggtga agatccgttt
    13321 caaaggcaaa ggtggccacg cagcttttcc tcatgaagcc aatgacgcct tggtggcggc
    13381 tagttacttt gtgacccaag tgcagtcagt tgttagccgc aatgtcaacc caatcgaggg
    13441 agcagtggtg accttcggcg tttttcaagc tggaaccacc aacaatgtca tcacagacac
    13501 agctttttta catggaacta ttcgtgcctt gacacaggac atgagtctct tggtgcaaaa
    13561 gagggtaaaa acagttgcag aaggggttgc agctgccttt gatatggaag tcgaagtgga
    13621 actcaaacaa ggtggatacc tacctgttga gaacaatcca gccttggcgc gtgaactgat
    13681 ggacttcttt gacgagaaag acggaatcga gttgattgat atcgaacctg ctatgacagg
    13741 cgaggacttt ggttatctcc tttcaaaggt tgatggcgtg atgttttggc taggtatcga
    13801 tagtccctac gctcttcatc accctcaaat gagtcctaag gaagaagtct tagctattgg
    13861 ggtggctgcg gtgtctagtt tcctgaaaaa gaaggcagca gaatagaggg cttgtctatg
    13921 aaatcggaat tacgcaagca agtcttgcat gaaatgaagg ctatatctca agagcaaaaa
    13981 caggctatag accaagcttt aaccgagcgg cttttacacc atcccttcta ccaagaagcc
    14041 aaggtcatcg caacctacct ctctttttct catgagtttc aaacgcggga actgattgag
    14101 caggcgctga aggacggcaa gaaggtttta atacccaaaa cttatcccaa ggggcgcatg
    14161 gagtttgtgg cctatgatcc gcaacagttg gtaaaaactt cctttgcctt actggagcca
    14221 cagggagatg tggaagtggt ggatgcatct cagattgatt tgattcatgt tcctggtctg
    14281 gcttttacga cgaaaggata tcggatagga tacggtggag gctattatga ccgctatctg
    14341 gaacattttt ctggtcgtac tttgagtacg atatatcctt gtcaaattca ggactttata
    14401 cctgaaaacc atgatattcc tgttcaggag gtattaattg atgaaggaaa tctttgatag
    14461 acgttaccct gtgacgagtt tcttcctctt agtgacggcc ttggtatttt tactaatgtt
    14521 ggtcactgca ggcggaaact ttgacagggc agatacatta tttcgatttg gagccatgta
    14581 tgggccagct attcgcctct ttcccgagca ggtttggcgt ctcttgtctg ccatttttgt
    14641 tcatattggg tgggaacatt tcattgttaa tatgctttca ctttattatc ttggaaggca
    14701 ggtagaggag attttcggtt ctaagcagtt tttctttctc tatcttttat caggaatgat
    14761 gggcaatctc tttgtttttg tatttagtcc taaatcctta gcagcaggcg cctctacctc
    14821 tctttatggg ctatttgccg cgattattgt tcttcgctat gcaactcgca atccttatat
    14881 ccaacagcta gggcaatcct atctgacact ttttgtggtt aacattattg gaagtgttct
    14941 gattccagga atcagcctag caggccatat cggtggtgca gttggtggcg catttctagc
    15001 agttatcttt ccagttagag gagaaaaacg gatgtataac accagccaga gattaggagc
    15061 ggtagtcttg ttcgtaggac tcgccatttt gcttttctac aagggaatgg gaatgtgacg
    15121 aagttgaaag aaaatttgtg taatgaaaaa agataaggca ttttttgaac cttatctttt
    15181 tattttattc cttctcagtc aattcttttc caagttggat gaggtaattc ttcaaatcat
    15241 ctttgacttg tggatgtttg agggcgtagt caatagatgt tttcataaag ccaaacttat
    15301 ccccaacgtc gtaacgagct cctgtaaatt cacgggcaaa aacacgctgt gttttattaa
    15361 gagtatcgat tgcatcagta agttggattt catttccagc accaggagct tggttttcga
    15421 ggatttggaa aatttcaggc gtgaggaggt agcgtccgat aatagcaagg tcgctaggag
    15481 cgtcctctgg agctggtttt tcaacaaagg tttcaacact gtaaagacca tcttttcctt
    15541 cgccttgcgg agcaataacc ccgtaagcag atacttcgtc atgagggact ggcatgacag
    15601 cgatagtaga cgcgtgggta cgcttgtagt catccatgag ttgtttggta agtggaacag
    15661 ccttttcgtc tgtgatatcc atcaagtcat caccaagcat aacgacaaaa ggttcatttc
    15721 cgacgaaagc cttggcttgc aaaacagcat ctccgagacc gcgtggatga gtttggcgga
    15781 taaaatgcag acgcatgcca gttgtttcat caactagctt caaaagatct gttttccctt
    15841 tttctttgag gttatattcc aattcgaagt ttgaatcgaa gtggtcctca atagaacgtt
    15901 ttgatttacc agtgacaact agaatatctt caatacctga tttgagagcc tcttccacga
    15961 taaactggat agttggtttg tctacgattg gcaacatttc tttggcaagg gccttggttg
    16021 ctggtaaaaa tcgagttcct agtccagcag cagggataac tgcctttcta acttttgatg
    16081 tcataaaaat cctttctata gagggttaag accactcatt ttctgcttta aattcattgt
    16141 tcatgatgtc ataaatggca tctttgatat tggttccgtg ataaataact tggtaaatag
    16201 cctgtgtaat gggcatatag actccaagtt cttgggctag ttcataggct gctcgagtcg
    16261 ttgaaattcc ttcgattacc atgcccatat tagcttctat atcagctagg gattctcctc
    16321 gtccgagagc atctccagct ctccagttac gagagtggat ggaagttccc gttacgatca
    16381 aatctcccac accagataag ccgctatagg tcaatggact ggccccgagt gctaccccta
    16441 ggcgggtgat ttctgctaaa cctcgagcga tgatggctgc cttagcatta tcaccaaatc
    16501 caagaccatg taaagctcca gcaccgacag caataatatt tttaagagca ccagcagttt
    16561 caaccccgat aacatccgta ttggtataaa gtcggaagta gtgattacta aatagctcct
    16621 gaacgtattg agctgtttgt aaatctttag aagcagcagt tattaaagtt aggtcacgca
    16681 caatggtctc ttctgcatga ctaggccctg aaacaacgac gatatcacta cggagatgtt
    16741 caggaatttc ttcttcaaga atggttgata atcgtttatg gctatcaggt tctaatccct
    16801 ttgatgcgtg catgatgata accttatggt ccaaggtttg tgcaacttgc tgggcaacaa
    16861 gtcgtgtcac ttttgttggg acaacaaaca aaatcgcatc cacatttttc aatgtttctg
    16921 ctaagtcggt gtaggcaatg atattttcgt ctagaacgac atctttaaag tagtgcttat
    16981 tagtatggtg tgtattaatt tcattgattt gctcgggaag atttccccaa atacgtacct
    17041 cgtgtccatt gtcatttaag acttgtgaaa gggcggttcc ccaagaacca ggccccaaga
    17101 cggcgacggt ttgtttttcc atgatttcct ccttgataga tttctttctc ttattctatc
    17161 atattctagg ggattttgca cttgcattac tggggtatgg tggaaaaata cacaaagacc
    17221 gagatgaagg gataaactct tagtctttag ttaaagtgag aagaaatctg atagatagtt
    17281 tgattcagta tatgcttata atagaggtaa acaactcagg aagttcttgt aggttgcgag
    17341 agtttggcga aaaaattaaa agattacgtt tggctaaaaa aatcagtcgt tcagaatttt
    17401 gtggtgatga gtctgaatta agtatccgtc aattaattag aattgaaaat ggagaatcca
    17461 gaccaacact aacaaagtta aaatatattg ctgaacgttt ggaggttgaa gattacaagt
    17521 tgatgccaag ttatatagag ttggataagg aatacctaga attgaagtat ttcttgatga
    17581 ggactcctac atacgaagat gaaactatcg cccaaaagaa agagagtgtt tttgataaga
    17641 tttttgaaga gtattatgat aggctacctg aggaagaaag atttatcatc ccaaattatt
    17701 catatctagc actagcgaac tacacagttc aaaaattacc agaaaagcta gttgaaatac
    17761 tgtccttctg gtgattttgc ttaatttctg atgttcgaga catgattgag gagatttgtc
    17821 ttgatttttt cctaaaaaag ggcagactcc tcccttggtt tgatgctcga tttttaaaat
    17881 aaggtgactt attctgttag gcagttttcc cctgccctct agctctatat ggaacaggga
    17941 gtatcagatt tatctggcag attgtatcaa ataaggcttg aaactgtttt gaataggcgt
    18001 atagacttga gaatttgaga atatgtcgtc tagcagtaga gacatatttt ccggcaatat
    18061 gaaggaagag acgtcgaaaa cgcttgatag tcaaggcctt tacttcatca ccagctagct
    18121 gctttaaaaa gaggtagaga ttataagcca gacagcccat catcatacgt acttcattct
    18181 taatcatggt cgaactatct gtcttatccc caaagaatcc tgctttcctt tctttgataa
    18241 agttttcagc attccctcgt tcacgataga attgaaacag ctcctcacta tctacactgc
    18301 ttaaattggt caaatgaact tttttacaac cataaaaaag agcacttaat cgtactcttc
    18361 tcttttaagg attagttgaa ttaattcaaa tccactagtt ctcctgtttc taggtagact
    18421 aggcgttcac aaatgttagc aatgtaatca gcgaagcgtt ccagatgccc tatgatataa
    18481 aggtattgag ttccattggg aattgaggtt tcttggtctt tcataagtcc aatgatttcc
    18541 tttgataagg cataataata ttggtcaatc tgttcatctt tttgagcaat actaatagct
    18601 tttgaggctt ggtgcaaagg aaaggcaacc aataaatcgg ctagcatgct gagggataat
    18661 ttacccattt ggtgtaactg ttcttcgtca ggggctagtt gattttcttt tagttgcaaa
    18721 acagctttgg caatgcctgc catatggtct cccatacgtt caaggtctga acaagaagac
    18781 atgatgctaa tcacaaatcg aaggtcagac acttgtggct gctgcaaggc caacaaacgg
    18841 gcacaggtca attcgatagc gctttgacct tggttgatag catgatcctt attgataatt
    18901 agctctgcca tctccttgtc tttggaggct aaggccagta aggcttttga agctgtttca
    18961 aggacaagtt gccctagtcc taaaaaggat tgttctaatt catgcaattc taagtcaaat
    19021 tgatttctca taggtttttc cttccttatc cgaaccgtcc tgaaatatag tcttctgtgc
    19081 gctgatcttt tggattggta aacacgtcaa cggtatctcc aaattcgcaa atttctcctg
    19141 ttaagaaaaa agcagtttta tctgaaatac gtgaagcttg ttgcatgtta tgggtaacaa
    19201 tgataatcgt ataatccttt tttagttgct gaatgaggtc ttcaattttt aaagtggaga
    19261 tagggtctaa ggctgaagtc ggctcatcca ttaacagaat atcaggttct actgctaaag
    19321 ctcgcgcaat gcaaaggcgt tgctgctgac cgccagataa ggacatagca ctctttttaa
    19381 gatcatcttt gacttcttcc caaatggctg ccccttttaa agatttctcc actaaggcat
    19441 ctaattgttt tttgtctcga ataccatgtg tccttgggcc ataagccacg ttatcataga
    19501 tagacatggc aaagggatta ggctgttgaa aaaccatccc tacacgctta cgtagctgat
    19561 taaggttgaa tttgctacta taaatatctt gctcatctaa gaggacttgg ccttcaatat
    19621 ggcaagaagg aaccaaatcg ttcatccggt taagggtttt tagaaaagtt gatttgccac
    19681 aaccagatgg gcctatcaag gcagtaatct gtctttctgg taattgaatc gaaatatttt
    19741 ttaaggcttg aaaatccccg taaaataagt ctaggtgtct gactgaaaat gttcccatac
    19801 taggaagctc ctttcacaag ttttcgagat aataagcttg atagagtatt tatcattaaa
    19861 acagtaataa tcaaaatcac accggtagca taggcttcat tgacatgtag cccctcactt
    19921 gacagcatat acatatgcag ggctagagaa cgacctgaag acatgagact acttggcgta
    19981 ttggtagagg tacctaatgt atacatgagg gcagctgtct caccaacgat acggccaata
    20041 gctagtatca ctccagctaa aatacctggc atggcaactg gtagaacaat tctaaaaaca
    20101 gtccgtaact tacctgcccc aagtccataa cttgcttgac gcatgctatc actaacagat
    20161 aaaagggctt cttctgttga gcgaataatg actggcaaca ccatgatgac tgaggttaag
    20221 attcctgata acagagagta ttgaaaacct aagaagacta caaagaagag catgccaaac
    20281 agaccaaaaa caatggaagg aatcccagac aaggtatctg aggccaatcg catgatttta
    20341 acacaaaggg aatctttttt tgtatattcc acaagataaa aaccagcaaa aatccctatg
    20401 ggcaaggcta aaagaagagc accaaagacc agaataacgg tggaaataat cgctggcata
    20461 agggaaatgt tctcagaagt ataagtccaa gaaaagaggg atagacttag atgaggtaag
    20521 cctttgatga ggataaaacc aatgattaaa aagagagagc caaaggttaa agctgaaaaa
    20581 caataaacga gaagttttag caggtattta ctcataagat gattttcctt tcaagtaggc
    20641 aaagtaggca ttaatcaaga gaataaggaa aaagagaact gctgaggttg caataagggc
    20701 ttccctatgc tgacctgatg cgtaagccat ttccagaaca atattggttg ttaaggttct
    20761 ggttcctgaa aagagtccac ttggaataat cggctggttg cctgccacca aaataactgc
    20821 catggtttca cctactgcgc gaccgattcc taaaataact gctgataaaa taccagatct
    20881 cgcagctggc aagatgacac taaaaatact ccgttcatga ctagctccta gagccaagct
    20941 accagaataa tacgttttgg gaactgttcg gatagcagat tctgacaaac tgataatggt
    21001 tggcaaaatc attattccta atagtaacga agcggttagg acactcatgc catttcctaa
    21061 aaagcttcta atccaaggca ccaataattg taggccgaaa aaaccataaa caatagatgg
    21121 aatggccgcc atcaagttga tagctgattt taagaagcca tagacgggct ttggacaata
    21181 ataaaccata aacaccgatg tcaagatgcc tgttggcacc ccaatcacaa tcgctcctaa
    21241 ggtaattaat aaggaaccaa cgatcattgg taaaatacca tagcttgccg gaatgttcgt
    21301 tggcgaccaa tcactgccta ataaaaaacg ggcaaagccg tagttagcca tgaaaggtaa
    21361 gccattacta aaaataaaga aacagattag caaaatagct acaacagcta ctgttgcact
    21421 catgaaaaaa attgccctaa aaactgcttc tttgaaggct tgttttatca catcttgtcc
    21481 tttctagtga agaaagtaag ggagatacga cacctcccta cttgccttct ttatcttatt
    21541 gtacgatgaa acgtctgcat ctctttagag atttatggag caaacatttt atttaatctt
    21601 gtcccaggtg gttaatttgc cactaaaaac gtctgcaagt tcagccatac tgacttggct
    21661 tgccttattg tcattattga ccacaacagc aataccgtct aaagcaatag catcatgggt
    21721 gagactctta ccttcttcag gagttaattc cctagaaacc ataccaatat cagcggtttt
    21781 ctccttaaca gcggtaatac ctgctgaaga cccattagag gtaatatcaa tcgtaacttc
    21841 tggattttct tttttataag cttctgctaa tttttccatt aaagaagata ctgaagtgga
    21901 acctacaaca gacaacttgc ctgataagtg ttggcttgta tattctgtgg tttcagtttt
    21961 agcttcaata aatttattat ctgtgaccac ttgttgacct tgtttggagt ggataaagct
    22021 gataaaatct tgacctagct tggaaagatt agaagaccaa acaatgttga agggacgttg
    22081 aagagggtat tcaccatcta aaactgtgtc tcgactagcc ttgacaccat caatctctaa
    22141 agccttgaca gatttcgtta aagatcccaa ggagatgtag ccgatagcat tagcattccc
    22201 ttgaactgct gagagaacac cttctgtact attttgaatc acagctgttt tggcagtgtt
    22261 gtcaattttt ttatcaccgt cttttttgag aatccctgtg atttctgtga aggcaccccg
    22321 tgttccagag ccattttctc gtgaaatcac ctcaatcgtt cctgaagctg actgtttgga
    22381 agcagctgac tgattgccac aggcaacaag cccaaatcct gataagccaa tggctgcaag
    22441 agtaagcatt tttttgaatt tcataataat cacctttatc tctatgtatt tttcttgtgt
    22501 aggcttacta catttatagt ctaacaagtc tttgtaaagg tttatccctg attcatgtaa
    22561 agattgtgta aagaatcaaa aaaagccact tttgaaaaat ggctgctcct aaaaatagct
    22621 ttaaaaatta ttagtcctgt gcgaaagatt ggttaggaag aaaaatcgtg aagcaactgc
    22681 ctctgccaag ctgacttgtc accgtgactt ggccacctaa taattgactg agttctttga
    22741 caatggcaag gccaagacca gtgccaccag tttgtctgct tcgaccttta ttaactcggt
    22801 aaaaacgttc aaaaatacga tcctgctcta attgactaat accaatccct gtatctgata
    22861 cagaaatctt aatgccttcg ttcacctttt gggtcttgac ctcaattttt cccccttgtt
    22921 cagtgtaacg gatggcattg gataaaagat tgagtaagat ttgggaaagt aattgactat
    22981 ctgatacgag ggtgacatca tctggcacct gcacctttag ctgtaaatcc ttcttcttga
    23041 gctgaggttg caagctttga gtcaaatcct gtacaaattc tgccaaagaa agggtcgtcc
    23101 attgtatagg catttgttga gccttagata aggtaagaag atgctcaaca atatgctcaa
    23161 gacgcaagct ttctttgtaa ataatgtcta gaaagtcatc cttgagcgct tcttcttcag
    23221 ctgacatccc cttaatggtt tcagcaaagc ccttaatcga agtaactggt gtcctcaatt
    23281 catgggaggc atttgagaca aaggctaaat ttaacttttc ataagttcta atcgttgtta
    23341 aatcatatag caagacgagc acagcttcca cagattgggt ggggctaaaa acgggaactg
    23401 ctgtcacttc taaaatcaag tcaccctcat gaaacccact tacttcttgt tttaaccttg
    23461 ttttttgatc aaaggcttgg tgaactaaat ttcgaatatc catccgtttg aggtcatcaa
    23521 gtgaacttat gtcgccgtcc acatcgggaa aataatgagg cagagagcga ctggataata
    23581 acatctgacc ttgagcggaa actaaaaacg tccccatggt taggtgcgac agaagaacct
    23641 ccattgtttc ggctaggtcc ttgtattgct gatcctgttg ggagactttg gtttttaggc
    23701 cagacacata ctgagccaaa gactttaagt cttcttgccc tttttctaaa aagtattcac
    23761 tactggtcaa gagaggttgg tgcaaggtct caaaagcaac ttcccatttc caaaggcaaa
    23821 agagccagta gccaccttgt cccaaagaaa gggctagaag aaagagaccg atgcctttac
    23881 tgatccaagt taatgccatc cctgcaatca gaatgaggct aacacttaga ttgactagcc
    23941 aaaattgaag gtagcgtttc atctataact ccttgaactt ataaccataa ccccgaatgg
    24001 ttcgaataaa ttgaggggct ttaggattgt cttcaatttt ttccctcaac ttaccaatat
    24061 gaacgtccac caaacgtgtt tcctgcccaa agtcataccc ccagatacgt tccaaaagac
    24121 gctctctagt cagtgtcatg ttgggatgtt tcataagata gagcaagagt tcaaattctt
    24181 ttggggtcaa actcagtaac ttattcgcct tgtagacttc atgacgctca gggtatactt
    24241 tcaaggtccc aaatagccaa gaatcgtcag cgatattatc tgaatcatct ccttcttgtt
    24301 ctcctttagt tcgcctgagg acagccttga cacgcgccag caattctcta gggctaaaag
    24361 gcttggtcag gtagtcatca gcccctaatt ccaaggccaa aaccttatca aattcatcac
    24421 ttttcgcaga aaccatcata attggagttt tgacgccttt ggctctcagc cgcttacaaa
    24481 cttccatgcc atctaattgt ggtaacatga tatcaagcaa gataaaatca aagggttctg
    24541 tttctgccaa agctaaggcc ttccgtccat ttgttaccaa ttgagtagaa aagccttcct
    24601 tacttaaatg gtagtcaagc aatttcagaa tgtgttcttc atcatccact aataagactt
    24661 gttttgtcat ctattatctc ctattggtaa cattataaca caattatcag aaatcctaac
    24721 attgctaaat tagattaaat ttgcttatca agactagtat ctggtcaaat gctcaatcat
    24781 ctccttgtgc tctggatagg tcgccagtag atctgccctt tcaaataatt caaaatcctc
    24841 aaattcaaaa ccaggagcaa caagacaaga aaccagagca tcatccttat caactgttga
    24901 tccccaaata gtgctcttag gaacacagta gtgaagttgt tgccctttgg atatgtccag
    24961 gcctaaagtg actgcttcgt agtgaccatc tgctgtaatc atgtgaacag taagtgggga
    25021 tcctgcatga aaataccaga tttcatctgc tgtcaatcgg tgaaaatgtg aaggattcgt
    25081 ttcttctaat aagaaataaa tactggtata aagcgccctt cccttaccag caaggtttat
    25141 agtgtctgaa gctttttttg tttgtctaaa atagccacct tcaacatggg gagctaactc
    25201 tagagttctt atcaagtctt ctttatccat cggggccaat gggttgaagt aactcttgtt
    25261 caaagtggtt ttacgatttc aagaactcct ctcagttctg aggacacggt aatgattgat
    25321 gcgacggaag taaaaatcaa tcgccctaaa aaaagaatta gcgaatgatt ctggtaaaaa
    25381 gaaatttcac gctatgaagg ctcaagcgat tgtcacaagt caagggagaa ttgtttctat
    25441 tgccatgata tgaagttgtt caaaatgagt tgcagaaata tcggacaagc tggtaaaatc
    25501 ttggctgaca gtgattatca agggctcatg aagatatatc ctcaagcaca aactccacgt
    25561 aaatccagca aactcaagcc gctaacagct gaagataaag cctgtaacca tgcgctatcc
    25621 aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatgttttca
    25681 acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat tgctggtatt
    25741 atcaatcatg aactaggatt ctagttttgc aggaagtcta ttatttggtt aggtgaatta
    25801 gtgaagtgtt taggcaagtg tctctggtta cgacgtcatg gcctctaaat cgattatatt
    25861 taggggtcat gactagtgaa gcagttagct agttcgcata taagcggcta gcgtctaaca
    25921 attaggaact ttagttccaa taactttaag attacgacgt tttaggacat aaatcgatca
    25981 tatttatgtc ctaaaactag tgaagcgcct agccaaagtc cgaataggat ttggcgttag
    26041 ttacttagat tgctttgcaa tcaagtaact ttggcgattt acatcttctc tggcgcttct
    26101 actccgagca agcgaagggc ttctttgaga acgactgcgg ttgcgtagct gagggctaga
    26161 cggctgtcgc gttctgggct ttcatccaag atacgcgtgt gtgcatagta tttgttaaag
    26221 gcttgagcca ggctaattgc aaatttggca atgatagaag gttcaaagtt atctgccgca
    26281 cgattgataa tacgtgggaa gtcttggatg agtttgatga tttcccagct ttcagtatca
    26341 ttcaagctat agttgccggc tgtttctggt ttgaagtctg ctttacgtaa gatagattgg
    26401 atacgagcgt aggcatattg tacataagga ccagtttccc cctcgaagga taccatagct
    26461 tctaggtcga aatcgtatcc atttgtacgg tcggtcttga ggtcatagaa cttaatggct
    26521 ccaaccccaa cagcatgtgc tacctgatct ttattttcca attcaggatt ttttgcttca
    26581 atttgggctt tggcacgact aatagcctct gcaacagtgg gctctagcaa gatgacattt
    26641 cctttacgag tagagagttt tttcccttct tttgtaacca aaccaaaagg aacgtgggta
    26701 atgtcgtcac tccagtcgta gcccatctct tgcaaaacag ctttgagttg tttaaagtgg
    26761 gcagattgtt cttgaccaac gacgtagata gatttggcaa attggtattc gtttttacgg
    26821 taaagggctg cagccaagtc acgtgtgata tagagagttg ctccatcaga tttcttgata
    26881 agggctggat gttcaattcc atatttctca agatttacaa cctgggcacc ttctgattca
    26941 acaagaaggc ctttttcaga aagaatgtct acaattgcat ccatcttatc gttgtagaag
    27001 gcttctccgt tgtagctgtc aaattcaacc tgcaattcat tgtaaaggcg gctaaattcc
    27061 accaaacttt catcacggaa ccattgccaa agagcgagag cttcctcgtc tccattttca
    27121 agtttacgga accattcgcg tgcttcttca tccaagctag ggtcattttc agcttcagcg
    27181 ttgatgcgga catagagttt aagaagttca tcgattggat gagcttttac agcttcttcg
    27241 ttcccccatt ttttgtaggc aacaatcaac atcccaaact gtttacccca gtctcccaaa
    27301 tggttgacct tgaccgtttg ataaccgatt ttttggaaaa tatgtgacaa gctatctccg
    27361 ataacagttg agcgcagatg accaatagag aatggtttag cgatattggg actagacatg
    27421 tcgataacaa cattttcttg tttaccaatg ttttggtcag catagtgttc tttttcagtg
    27481 ataacagctt gtaatacttg agcagaaata gcagatttat caaggaaaaa gttgacgtaa
    27541 ggtcctgttg cgacaacctt ttcaaaggat tggctgttaa tcttttcagc tagttcagcc
    27601 gcaatcattt gaggtgcttt acgttcgact tttgcaagag aaaaagcagg gaaagcaatg
    27661 tctcccattt ctgagttttt aggggtttcc agtaaattta aaatagcctc ttggtccagg
    27721 ctatcaatga cgctagccaa ctcgctagca atcaattctt ttgtattcat taagagctcc
    27781 tttttggact tttctactat tttatcacaa ttttaaagaa agaagaaaaa atttttgaaa
    27841 tctcctattt ttttggtata atatagttat aaaaatagtt ataaatattc acataagagg
    27901 attttatgag aaaaagagat cgtcatcagt taataaaaaa aatgattact gaggagaaat
    27961 taagtacaca aaaagaaatt caagatcggt tggaggcgca taatgttttt gtgacgcaga
    28021 caaccctgtc tcgtgatttg cgcgaaatcg gcttgaccaa ggtcaagaaa aatgatatgg
    28081 tgtattatgt actagcaaat gagacagaaa agattgattt ggtggaattt ttgtctcatc
    28141 atttagaagg tgttgcaaga gcagagttta ccttggtgct tcataccaaa ctgggagaag
    28201 cctctgtttt ggcaaatatt gtagatgcaa acaaggatga atggatttta ggaacagttg
    28261 ctggtgccaa taccttattg gtcatttgtc gagatcagca cgttgccaaa ctcatggaag
    28321 atcgtttgct agatttgatg aaagataagt aaggtcttgg gagttgctct caagacttat
    28381 ttttgacaag gagagacgga aaatggcgac agaaaagcta tcacccggca tgcaacagta
    28441 tgtggatatt aaaaagcaat atccagatgc ttttttgctc tttcgtatgg gtgattttta
    28501 tgaattattt tatgaggatg cggtcaatgc tgcgcagatt ctggaaattt ccttaacgag
    28561 tcgcaacaag aatgccgata atccgatccc tatggcgggt gttccctatc attctgccca
    28621 acagtacatt gatgtcttga ttgagcaggg ttataaggtg gctatcgcag aacagatgga
    28681 agatcctaaa caagcagttg gggttgtgaa acgagaggtt gttcaagtca ttacgccagg
    28741 gacagtggtc gatagcagta agccagacag tcagaacaac ttcttggttg ccatagatcg
    28801 tgatggcagt cagtttgggc tagcttatat ggacctggtg acgggtgact tttatgtgac
    28861 aggtcttttg gatttcacgc tggtttgtgg ggaaatccgt aatctcaagg cgcgagaagt
    28921 ggtgctgggc tatgacttgt ctgaggaaga ggaacaaatc cttagtcgtc agatgaatct
    28981 ggtactttcc tatgaaaaag aaggctttga agaccttcat ttactggatt cacgattggc
    29041 atctgtggag caagcggcat ctagtaaatt gctccagtat gttcatcgga ctcagatgag
    29101 ggaattgaac cacctcaagc ctgttatccg ctatgaaatc aaagattttt tgcagatgga
    29161 ttatgcgacc aaggctagtc tggatttggt tgagaatgct cgttcaggca agaagcaagg
    29221 cagtcttttc tggcttttgg atgaaaccaa aacagccatg ggtatgcgtc tcttgcgttc
    29281 ttggattcat cgccccctga ttgataagga gcgaatcatc caacgtcaag aagtggtgca
    29341 ggtcttcctc gaccatttct ttgagcgcag tgatttgaca gacagtctca agggtgttta
    29401 tgacattgaa cgcttggcta gtcgggtttc ttttggcaag accaatccca aggatctctt
    29461 gcagttggcg actaccttgt ccagtgtgcc acggattcgt gcgattttag aagggatgga
    29521 gcaacctgct ctagcctatc tcatcgcaca actggatgga attcctgagt tggagagttt
    29581 gattagtgca gcgattgctc ctgaagctcc tcatgtgatt acagatgggg gaattatccg
    29641 aactggattt gatgagactt tagacaagta ccgtcgcgtt ctcagagaag ggactggttg
    29701 gattgctgag attgaggcta aggagcgaga aaactctggt atcagcacgc tcaagattga
    29761 ctacaataaa aaggatggct actattttca tgtgaccaat tcgcaactgg gaaatgtgcc
    29821 agcccacttt ttccgcaagg cgacgctgaa aaactcagaa cgctttggaa ccgaagaatt
    29881 agcccgtatc gagggagata tgcttgaggc gcgtgagaag tcagccaacc tcgaatacga
    29941 aatatttatg cgcattcgtg aagaggtcgg caagtacatc cagcgtttac aagctctagc
    30001 ccaaggaatt gcgacggttg atgtcttaca gagtctggcg gttgtggctg aaactcagca
    30061 tttgattcga cctgagtttg gtgacgattc acaaattgat atccggaaag ggcgccatgc
    30121 tgtcgttgaa aaggttatgg gggctcagac ctatattcca aatacgattc agatggcaga
    30181 agataccagt attcaactga ttacagggcc aaacatgagt gggaagtcta cctatatgcg
    30241 tcagttagcc atgacggcgg ttatggccca gctgggttcc tatgttcctg ctgaaagcgc
    30301 ccatttaccg atttttgatg cgatttttac ccgtatcgga gcagcagatg acttggtttc
    30361 gggtcagtcg acctttatgg tggagatgat ggaggccaat aatgccattt cgcatgcgac
    30421 caagaactct ctcattctct ttgatgaatt gggacgtgga actgcaactt atgacgggat
    30481 ggctcttgct cagtccatca tcgaatatat ccatgagcac atcggagcta agaccctctt
    30541 tgcgacccac taccatgagt tgactagtct ggagtctagt ttacaacact tggtcaatgt
    30601 ccacgtggca actctggagc aggatgggca ggttaccttc cttcacaaga ttgaaccggg
    30661 accagctgat aaatcctacg gtatccatgt tgccaagatt gctggcttgc cagcagacct
    30721 tttagcaagg gcggataaga ttttgactca gctagagaat caaggaacag agagtcctcc
    30781 tcccatgaga caaactagtg ctgtcactga acagatttca ctctttgata gggcagaaga
    30841 gcatcctatc ctagcagaat tagctaaact ggatgtgtat aatatgacac ctatgcaggt
    30901 tatgaatgtc ttagtagagt taaaacagaa actataaaac caagactcac tagttaatct
    30961 agctgtatca aggagacttc tttgacaatt ctccactttt ttgctagaat aacatcacac
    31021 aaacagaatg aaaaggagct gacgcattgt cgctcccttt tgtctatttt taaggagaaa
    31081 gtatgctgat tcagaaaata aaaacctaca agtggcaggc cctggcttcg ctcctgatga
    31141 caggcttgat ggttgctagt tcacttctgc aaccgcgtta tctgcaggaa gtcttaggcg
    31201 ccctccttac tgggaaatat gaagctattt atagtatcgg ggcttggttg attggtgtgg
    31261 ccgtagtcgg tctagttgct ggtggactca atgttgtcct cgcagcctat attgcccaag
    31321 gagtttcatc cgaccttcgg gaggatgcct tccgtaaaat tcaaaccttt tcttatgctg
    31381 atattgaaca atttaatgcg ggaaatctag tcgttcgaat gacaaatgat atcaaccaga
    31441 ttcagaacgt tgtcatgatg accttccaga ttcttttcag acttcccctc ttgttcatcg
    31501 gttcgtttat cctagcggtt caaaccttac cttctctgtg gtgggtgatt gttctcatgg
    31561 tagtcttgat ttttggtttg actgctgtca tgatgggaat gatggggcct cgttttgcca
    31621 agtttcaaac ccttcttgag cgtatcaatg ccattgccaa ggaaaattta cgtggcgttc
    31681 gtgtggtcaa gtcctttgtc caagaaaaag agcaatttgc taagtttaca gaggtctcag
    31741 acgagcttct tggtcaaaac ctttacattg gttatgcctt ttcagtagtg gaacccttta
    31801 tgatgttggt tggttacggg gcggtcttcc tctctatttg gctggtcgcg ggaatggttc
    31861 agtcggatcc gtctgttgtt ggttccatcg cttcttttgt taattaccta agccagatta
    31921 tctttaccat tgttatggtt ggatttttgg gaaattctgt cagccgtgcc atgatttcca
    31981 tgcgtcgtat tcgagaaatt cttgacgcag agccagctat gaccttcaag gatatcccag
    32041 atgaagagtt ggttggaagc cttagctttg aaaatgtgac ctttacctat ccaatggaca
    32101 aggaaccgat gctgaaagat gtgagcttta ctattgaacc tggtcaaatg gttggtgtag
    32161 ttggagcgac tggtgcagga aagtcaactt tggctcaatt gattccacgt ctctttgatc
    32221 cacaggacgg ggccattaaa atcggtggca aggatattcg agaagtgagt gaaggaaccc
    32281 tgcgtaaaac agtttccatc gttctccaac gtgccattct ttttagtgga acgattgcag
    32341 ataacttgag acaggggaag ggaaatgcta ctctatttga aatggagcgc gcagccaata
    32401 ttgcccaggc tagtgaattc attcatcgta tggagaaaac ctttgaaagt ccagttgaag
    32461 aacggggaac caatttctct ggtggacaaa aacaaaggat gtcgattgcg cgtgggattg
    32521 tcagcaatcc acgtattctg atttttgatg attcgacctc agccttggat gccaaatcag
    32581 agcgcttggt gcaagaagct ttgaataagg acttgaaggg tacgacaacc attattattg
    32641 ctcaaaaaat tagctcggtt gtccatgcag acaagatctt ggttctaaat caaggacgat
    32701 tgattggtca aggcacgcat gcagacttgg ttgccaacaa tgccgtttac cgtgaaatct
    32761 atgaaacaca gaaatgaaag acaaactata agaaaagtca atagttttat ctaaactatt
    32821 tcttatttca atttgatgat ttggcgatga ttttagagca cggcaaaaag cccttgaaaa
    32881 agtccatttt ttcaaaggta atcctgtgtt gatttcagaa attacatcac tttttgttcg
    32941 tcaaatggca gctctttttt aggatataaa acagggttcg gataagtttt tttgcaaggt
    33001 ggatgatggc tacattgtaa tgttttcctt attctaactt agtcttaaga taggtcttga
    33061 aagtaggtga aaagcgaggg catgctttgg cagcttgtat gagtacctac cgcagatgag
    33121 gggaaccccg tttgaccatt cttccagcta aatcaatctg acctgactga taaatagaag
    33181 aatccagtcc agcgaaagct tgtaattgag caagattatc aaaggcatga atatttcgaa
    33241 tctcggctaa aatgaccgcc cctaaacgat tcccaatccc agtaaccgtc gtgatgatcg
    33301 agttgaactc agccatcaag tcattgacac atgtttccgc cttgtcaatg agcctcttgt
    33361 aatgtttgat gttttcatta cacgagataa aacgtttatg cgttatcaaa ctcatgacca
    33421 attgaaacaa aaagctgtgg ttagatcctt tcggaaattg tcaagcgatt ggaggaaatg
    33481 aactaatcca cagcggctta ttccaagtat accacttggg ctttggcagt agctaactgc
    33541 gctaaatata atataaggag gagtaaaatg aagacagttc aatttttttg gcattatttt
    33601 aaggtttaca agttctcatt tgtagttgtc atcctgatga ttgttctggc gacttttgcc
    33661 caagccctct ttccagtctt ttctggacaa gcggtgacgc agctagccaa tttagttcaa
    33721 gcttatcaaa atggcaatcc agaacttgta tggcaaagcc tatcaggaat catggtcaat
    33781 cttggcctgc tggttttggt tctatttatc tctagtgtaa tatacatgtg tctcatgacg
    33841 cgcgtgattg cagaatcgac caacgagatg cgcaaaggcc tctttggtaa gcttgctcag
    33901 ttgacggttt ctttctttga ccgtcgacaa gatggcgata tcctgtctca ttttaccagt
    33961 gatttggata atatcctcca agcctttaac gaaagcttga ttcaggtcat gagcaatatt
    34021 gttttataca ttggtctgat tcttgtcatg ttttcgagaa atgtgacgct ggctctcatc
    34081 accattgcca gcaccccatt ggctttcctt atgctgattt tcatcgtgaa aatggcacgc
    34141 aaatacacca acctccagca gaaagaggta gggaagctca acgcctatat ggatgagagc
    34201 atctcaggcc aaaaagccgt gattgtgcaa ggaattcaag aggatatgat ggcaggattt
    34261 cttgaacaaa atgagcgcgt gcgcaaggca acctttaaag gaagaatgtt ctcaggaatt
    34321 cttttccctg tcatgaatgg gatgagcctg attaatacag ccatcgtcat ctttgctggt
    34381 tcggctgtac ttttgaatga taagtctatt gaaacaagta cagccctagg tttgattgtt
    34441 atgtttgcac aattttcaca gcagtactac cagcctatta tccaagttgc agcgagttgg
    34501 ggaagccttc agttggcctt tactggagct gaacgaattc aggaaatgtt tgatgcagag
    34561 gaggaaatcc gacctgaaaa ggctccaacc ttcactaagt tgcaagaaag tgttgaaatc
    34621 cgtcatatcg atttttcata cttgcctgat aaacctattt tgaaagatgt cagcatttct
    34681 gcccctaaag gccagatgac agcagttgtt gggccgacag gttcaggaaa aacgactatt
    34741 atgaacctca tcaatcgctt ttatgatgtt gatgctggtg gtatttattt tgatggtaaa
    34801 gacattcgtg gctatgactt agatagtctt agaagcaagg tgggaattgt attgcaagat
    34861 tcggtcttgt ttagcggaac gattcgagac aatatccgat ttggtgtgcc agatgctagt
    34921 caggaaatgg ttgaggtagc agcaaaagca acccacattc acgactatat cgaaagtttg
    34981 cctgataagt acgatactct tattgatgat gaccagagca tcttttcaac agggcagaag
    35041 caattgattt caatcgctcg aaccctgatg acagatccag aagttctcat tctcgatgaa
    35101 gcaacttcaa acgtagatac ggtgacagaa agcaagattc agcatgccat ggaggtggtt
    35161 gtagcaggta gaactagttt cgtcattgcc caccgcttga aaaccattct caatgcagat
    35221 cagattattg tccttaaaga tggagaagtc attgaacgtg gtaaccacca tgaacttttg
    35281 aagctaggtg gcttttattc agaactctat cacaatcaat ttgttttcga ataagaaaga
    35341 agttgtccta tgtgggcagc tttttcttgt ccataaaaaa tgtttatcac agccttaaaa
    35401 aaaacatatt agacgaaagt cattttgagt gatatgatag gactatcgtt agcattcgaa
    35461 aggagaggca tcatggctag aacggttgta ggagttactg caaatctatg tcccgtagac
    35521 gcagaaggca aaatcattca ttcatctgta tcttgtagat tcgcagagat cattcgtcaa
    35581 gtcggtggtc tccctttagt cattcctgtt ggtgatgagt cagttgtacg tgattatgtg
    35641 gaaatgattg acaaactcat tttgacagga ggccaaaatg ttcatcctca gttttatgga
    35701 gagaaaaaga ccgtcgagag cgatgattac aatctggtcc gtgacgaatt tgaattggca
    35761 ctcttgaagg aagcgcttcg tcagaataaa ccaattatgg caatctgtcg cggtgtccaa
    35821 cttgtcaatg ttgcctttgg tggaaccctc aatcaagaaa tcgaaggtca ctggcaagga
    35881 ctacctttcg ggacatctca ctctattgag acagtagaag gaagcgtggt ggctaagcta
    35941 tttgggaaag aaagtcaggt caattcagtc catcgtcaaa gcattaaaga tttggcaccc
    36001 aatttccgtg taactgctat tgattcaaga gaccaaacta ttgaagcgat tgagtctatc
    36061 gacgagcacc gcattatcgg tttacagtgg catccagagt ttctggttaa tgaagaagat
    36121 ggcaatttag aattatttga gtatttattg aatgaactgt aacgattgga acatctggtc
    36181 gttctttctt ttattttttc aaaattttta gaatgtggta ttttacgcaa acgtttgaat
    36241 tctgataaaa attggagaaa ttcgacaaaa aaacttgaaa aaaacgaagg taagcgttat
    36301 gatagaaaag aagaaatatt ggaggaagaa catgtcacat attaaatttg attattcaaa
    36361 agttttagac aaatttgttg caccacatga agtggaatac atgcaatcac aagtaacagc
    36421 agcagatgaa ttgatccgta aaggaactgg tgctggtagc gacttcttgg gatggttgga
    36481 ccttcctgaa aaatacgacc gcgaagaatt tgaccgcatc ttgaaagctg ctgagcaaat
    36541 caagtcagac agcgatgttt tggttgtgat cggtattggt ggatcttacc ttggtgcaaa
    36601 agcagcaatc gacttcttga atcaccattt tgctaacttg caaacaaaag aagaacgcaa
    36661 agctccacaa atcctttacg caggaaactc aatctcatct acttaccttg ctgacttggt
    36721 agagtatgtt gcagacaaag acttctcagt aaacgtaatt tctaaatcag gtacaacaac
    36781 tgaaccagcg attgctttcc gtgtctttaa agaactcttg gttaagaaat acggtcaaga
    36841 agaagctaac aaacgtatct atgcaacaac tgaccgccaa aagggtgctg ttaaggttga
    36901 agcagacgct aacggttggg aaacatttgt tgttccagat gatatcggtg gacgcttctc
    36961 agtattgaca gccgttggtt tgcttccaat cgcagcatca ggagctgaca tcaaagctct
    37021 tatggaaggt gcgaatgcag ctcgcaaaga ctacacttca gacaaaatct ctgaaaacga
    37081 agcttaccaa tacgcagctg ttcgtaacat cctttatcgt aaaggctatg caactgagat
    37141 cttggtaaac tatgagccat cacttcaata cttctcagaa tggtggaaac aattggctgg
    37201 tgaatcagaa ggaaaagacc aaaaaggtat ctacccaact tcagccaact tctcaactga
    37261 cttgcactca cttggtcaat ttatccaaga aggaactcgt atcatgtttg aaacagttgt
    37321 ccgtgttgac aaacctcgta aaaacgtgct tattcctact ttggaagaag accttgacgg
    37381 acttggttac cttcaaggaa aagacgttga ctttgtaaac aaaaaagcaa ctgacggtgt
    37441 tcttcttgcc cacacagatg gtgatgtacc aaacatgtat gtgactcttc cagagcaaga
    37501 cgctttcact cttggttaca ctatctactt cttcgaattg gcaattgccc tttcaggtta
    37561 cttgaatgct atcaacccat ttgaccaacc aggtgttgaa gcttataaac gtaacatgtt
    37621 tgcccttctt ggaaaaccag gatttgaaga attgagcaaa gaacttaacg cacgtctata
    37681 atagaagaaa agagtggttt acccactctt tttactctct ttatccatag aaattggact
    37741 cagtcaagac ttgtgatata atatagaaag caaaaaggca gatgcctaga taataggaga
    37801 aactatgtca aaagatatcc gcgtacgtta cgcaccaagt ccaacaggac tactacacat
    37861 cggaaatgct cgtacagcat tgtttaatta cttgtatgcg cgccatcatg gtggaacatt
    37921 tctcatccgt atcgaagata ctgaccgtaa acgccatgtc gaggatggtg aacgttcaca
    37981 acttgaaaac cttcgctggt taggcatgga ttgggatgaa agtccagaat cacatgagaa
    38041 ttatcgccag tctgagcgtt tagacttgta tcaaaaatac attgaccaac tattagctga
    38101 aggaaaagcc tataaatctt acgttacaga agaagagttg gcagctgaac gcgaacgcca
    38161 agaagtagct ggcgaaacac cacgctacat caatgaatac cttggtatga gtgaagaaga
    38221 aaaagcagct tacatcgcag aacgtgaagc agcagggatc atcccaactg ttcgtttggc
    38281 tgtcaatgag tcaggtatct acaagtggca tgatatggtc aaaggcgata tcgaatttga
    38341 aggtggcaat atcggtggtg actgggttat ccaaaagaaa gacggttacc caacttacaa
    38401 ctttgccgtt gttatcgatg accacgatat gcaaatctct catgttatcc gtggagatga
    38461 ccatattgct aatacaccaa aacagcttat ggtctatgaa gctcttggtt gggaagctcc
    38521 agagttcggt cacatgacct tgattatcaa ctctgagact ggtaagaagt tatctaaacg
    38581 tgatactaat acacttcaat ttatcgaaga ttaccgaaag aaaggttacc ttccagaagc
    38641 agtctttaac tttattgctc ttcttggttg gaacccaggt ggtgaagatg agattttctc
    38701 tcgtgaagaa ctcattaaac tttttgatga aaaccgcctc agcaagtcac cagcagcctt
    38761 tgatcagaaa aaactagact ggatgagcaa tgattatatc aagaatgcag accttgaaac
    38821 catctttgaa atggcaaaac cattcttaga ggaagcaggc cgtttgactg acaaggctga
    38881 aaaattagtt gagctctata aaccacaaat gaaatcagta gatgagatta tcccattgac
    38941 agatcttttc ttctcagatt tcccagaatt gacagaagca gagcgcgaag tcatgacggg
    39001 tgaaacagtt ccaacagttc ttgaagcatt caaagcaaaa cttgaagcga tgacagatga
    39061 taaatttgtg acagaaaata tcttcccaca aattaaagca gtccaaaaag aaacaggtat
    39121 taaagggaaa aatcttttca tgcctattcg tatcgcagtt tcaggcgaaa tgcatgggcc
    39181 agaattacca gatacaattt tcttgcttgg acgtgaaaaa tcaattcagc atatcgaaaa
    39241 catgctaaaa gaaatctcta aataaagagg atacaataat ggacatctgg gaaaagatgt
    39301 acgaagaagc acagaaacta tacaatccac atgaagtatc agactttgtt tatgccaatc
    39361 atgttgtagc tgcagtagaa gcagaagatg gacaaatatt tacaggattc tgtatggagg
    39421 gaacctgtgg tgttttccat ctctgcgcag aacgggcggc cctcttcaat atgtaccaat
    39481 tttcaggaca aactaaggtt aagaaagtat tagcctttcg agataaacca ccttatggtg
    39541 gaagttcagc catgccttgc ggtgcttgta gaaaattcct tttagagctt aatgctgaaa
    39601 ataaagatgc agagttcatg atggactata atataagaaa aacagttaaa gtcgcagaac
    39661 taatccctta ttggtgggga gaagaacgtg cttctaagtt taatgagcga tagaagagtc
    39721 agtataattc tggctctttt tactataagt tgaaaaaata
//
LOCUS       DAVLVZ010000015        39574 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000015 DAVLVZ010000000
VERSION     DAVLVZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.015"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            643..2796
                     /locus_tag="VYN77_000712"
     CDS             643..2796
                     /locus_tag="VYN77_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1558198.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMAFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2809..4158
                     /locus_tag="VYN77_000713"
     CDS             2809..4158
                     /locus_tag="VYN77_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1558199.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQSEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4328..5035
                     /locus_tag="VYN77_000714"
     CDS             4328..5035
                     /locus_tag="VYN77_000714"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1558200.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5237..8962
                     /locus_tag="VYN77_000715"
     CDS             5237..8962
                     /locus_tag="VYN77_000715"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1558201.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELKAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9055..10497
                     /locus_tag="VYN77_000716"
     CDS             9055..10497
                     /locus_tag="VYN77_000716"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1558202.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFFFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPFSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10534..11556
                     /locus_tag="VYN77_000717"
     CDS             10534..11556
                     /locus_tag="VYN77_000717"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1558203.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYIKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11553..12098
                     /gene="purN"
                     /locus_tag="VYN77_000718"
     CDS             11553..12098
                     /gene="purN"
                     /locus_tag="VYN77_000718"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1558204.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12182..12691
                     /locus_tag="VYN77_000719"
     CDS             12182..12691
                     /locus_tag="VYN77_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1558205.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12716..14263
                     /gene="purH"
                     /locus_tag="VYN77_000720"
     CDS             12716..14263
                     /gene="purH"
                     /locus_tag="VYN77_000720"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1558206.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKASPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMALGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14385..15647
                     /gene="purD"
                     /locus_tag="VYN77_000721"
     CDS             14385..15647
                     /gene="purD"
                     /locus_tag="VYN77_000721"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1558207.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            16050..16538
                     /gene="purE"
                     /locus_tag="VYN77_000722"
     CDS             16050..16538
                     /gene="purE"
                     /locus_tag="VYN77_000722"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1558208.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16525..17616
                     /gene="purK"
                     /locus_tag="VYN77_000723"
     CDS             16525..17616
                     /gene="purK"
                     /locus_tag="VYN77_000723"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1558209.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPVADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17626..17853
                     /locus_tag="VYN77_000724"
     CDS             17626..17853
                     /locus_tag="VYN77_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV1558210.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17916..19214
                     /gene="purB"
                     /locus_tag="VYN77_000725"
     CDS             17916..19214
                     /gene="purB"
                     /locus_tag="VYN77_000725"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1558211.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19269..23207)
                     /gene="strH"
                     /locus_tag="VYN77_000726"
     CDS             complement(19269..23207)
                     /gene="strH"
                     /locus_tag="VYN77_000726"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1558212.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEALKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23522..24238)
                     /locus_tag="VYN77_000727"
     CDS             complement(23522..24238)
                     /locus_tag="VYN77_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1558213.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDREH"
     gene            24586..26373
                     /locus_tag="VYN77_000728"
     CDS             24586..26373
                     /locus_tag="VYN77_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1558214.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26370..26846
                     /locus_tag="VYN77_000729"
     CDS             26370..26846
                     /locus_tag="VYN77_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1558215.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPIEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26873..27778
                     /locus_tag="VYN77_000730"
     CDS             26873..27778
                     /locus_tag="VYN77_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1558216.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERYDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQHVVDLVTEYQWIADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27765..28580
                     /locus_tag="VYN77_000731"
     CDS             27765..28580
                     /locus_tag="VYN77_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1558217.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATMINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFAFGM"
     gene            28587..28991
                     /locus_tag="VYN77_000732"
     CDS             28587..28991
                     /locus_tag="VYN77_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1558218.1"
                     /translation="MTKSLILVSHGRFCEELKGSTEMIMGPQDNIYTVALLPEDGPED
                     FTAKFEAAIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIELYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29091..30257
                     /locus_tag="VYN77_000733"
     CDS             29091..30257
                     /locus_tag="VYN77_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1558219.1"
                     /translation="MLHYTKEDLIELGAEITTREIYQQPDVWKEAFESYQEKREEIAA
                     FLQGIADKHDYIKVILTGAGTSAYVGDTLVPYFKEVYDERKWNFNAIATTDIVANPET
                     YLKKDVATVLVSFARSGNSPESVATVDLAKALVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAASNDAGFAMTSSFTSMMLTALLVFDPTEFAVKAERFEVVSSLARKVLDNA
                     EDVKELVDLDFNRVIYLGAGPYFGLSHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INEDTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30373..31410
                     /locus_tag="VYN77_000734"
     CDS             30373..31410
                     /locus_tag="VYN77_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1558220.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            31618..32472
                     /locus_tag="VYN77_000735"
     CDS             31618..32472
                     /locus_tag="VYN77_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1558221.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            32609..32866
                     /locus_tag="VYN77_000736"
     CDS             32609..32866
                     /locus_tag="VYN77_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV1558222.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(32908..33270)
                     /locus_tag="VYN77_000737"
     CDS             complement(32908..33270)
                     /locus_tag="VYN77_000737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558223.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFWGSVHSTPDTMR
                     FFNFRDFLQLLLTHIKLF"
     gene            complement(33324..33620)
                     /locus_tag="VYN77_000738"
     CDS             complement(33324..33620)
                     /locus_tag="VYN77_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1558224.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(33610..33771)
                     /locus_tag="VYN77_000739"
     CDS             complement(33610..33771)
                     /locus_tag="VYN77_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV1558225.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            33841..34605
                     /locus_tag="VYN77_000740"
     CDS             33841..34605
                     /locus_tag="VYN77_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1558226.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(34845..34997)
                     /locus_tag="VYN77_000741"
     CDS             complement(34845..34997)
                     /locus_tag="VYN77_000741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558227.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            35215..36594
                     /locus_tag="VYN77_000742"
     CDS             35215..36594
                     /locus_tag="VYN77_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1558228.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            36608..37273
                     /locus_tag="VYN77_000743"
     CDS             36608..37273
                     /locus_tag="VYN77_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1558229.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            37387..37482
                     /locus_tag="VYN77_000744"
                     /pseudo
     CDS             37387..37482
                     /locus_tag="VYN77_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            37673..38122
                     /locus_tag="VYN77_000745"
     CDS             37673..38122
                     /locus_tag="VYN77_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1558230.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLMIGGEQVEILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGVVNCGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDAFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            38279..38689
                     /locus_tag="VYN77_000746"
     CDS             38279..38689
                     /locus_tag="VYN77_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1558231.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            38805..>39574
                     /gene="pavB"
                     /locus_tag="VYN77_000747"
     CDS             38805..>39574
                     /gene="pavB"
                     /locus_tag="VYN77_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1558232.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRA"
BASE COUNT        10935 a         7675 c         9254 g        11710 t
ORIGIN      
        1 gaaaaaaatt tttaaagtct tcggagaaga aaacagaaaa atctgttaaa aaataatcaa
       61 aactataaat gatgaatctg aatcaaaatt attttgcgca tgtaaagagg agtcttatag
      121 taacgagtca aaaaaggagt aactatgaat cgtaatttag aacggtgtta tctattctga
      181 ctaggaatag atcataccag aggtagctta gaaatagcag agacattaga aattgaagta
      241 ataaatagga tgtcgtaagt gttactatca atgatttatt tgtttcaagc ttgcctaggg
      301 tgacagtaaa aaatcaattt cctttcaata gcatattttt agtgggcagg actcttgttc
      361 tgcctatttt tttatccaaa aagtgcagtt gggagggaga taggctcatt tgggaaggaa
      421 gtccagtttt tgtttagtga ttgaggtaag atagttgtta tcagatgagt ttatactctt
      481 cgaaaatcaa attcaaacca cgtcaacgtc gccttgacgt atatatgtga ctgacttcgt
      541 cagtcctatc tacaacctca aaacagtgtt ttgagcagcc tgcggctagt ttcctagttt
      601 gctctttgat tttcattgag tattagggaa aaggagatga atatgaaatt tgggaaacgt
      661 cactatcgtc cgcaagtgga tcagatggac tgcggtgtag cttcattagc catggttttt
      721 ggctactatg gtagttatta ttttttggct cacttgcgag aattggctaa gacgaccatg
      781 gatgggacga cggctttggg cttggtcaag gtggcagagg agattggttt tgagacgcga
      841 gccattaagg cggatatgac gctttttgac ttgccggatt tgacttttcc ttttgttgcc
      901 catgtgctta aggaagggaa attgctccac tactatgtgg tgactgggca ggataaggat
      961 agcattcata ttgccgatcc agatcccggg gtgaagttga ctaaactgcc acgtgagcgt
     1021 tttgaggaag aatggacagg agtgactctt tttatggcac ctagtccaga ctataagcct
     1081 cataaggaac aaaaaaatgg tctgctctct tttatcccta tattagtgaa gcagcgtggc
     1141 ttgattgcca atatcgtttt ggcaacactc ttggtaaccg tgattaacat tgtgggttct
     1201 tattatctgc agtctatcat tgatacctat gtgccagatc agatgcgttc gacactaggg
     1261 attatttcta ttgggctagt catcgtctac atcctccagc aaatcttgtc ttacgctcag
     1321 gagtatctct tgcttgtttt ggggcaacgc ttgtcgattg acgtgatttt gtcctatatc
     1381 aagcatgttt ttcacctccc tatgtccttc tttgcgacac gcaggacagg ggagatcgtg
     1441 tctcgtttta cagatgctaa cagtattatc gatgcgctgg cttcgaccat tctttcgatt
     1501 ttcctagatg tgtcaacagt tgtcattatt tcccttgttt tattttcaca aaataccaat
     1561 ctctttttca tgactttatt ggcgcttcct atctacacag tgattatctt tgcctttatg
     1621 aagccgtttg aaaagatgaa tcgggacacc atggaagcca atgcggttct gtcttcttct
     1681 atcattgagg acatcaacgg tattgagact atcaagtcct tgaccagtga aagtcagcgt
     1741 taccaaaaaa ttgacaagga atttgtggat tatctgaaga aatcctttac ctatagtcga
     1801 gcagagagtc agcaaaaggc tctgaaaaag gttgcccatc tcttgcttaa tgtcggcatt
     1861 ctctggatgg gggctgttct ggtcatggat ggcaagatga gtttggggca gttgattacc
     1921 tataatacct tgctggttta ctttaccaat cctttggaaa atatcatcaa tctgcaaacc
     1981 aagcttcaga cagcgcaggt tgccaataac cgtctaaatg aagtgtatct agtagcttct
     2041 gagtttgagg agaagaaaac agttgaggat ttgagcttga tgaagggaga tatggccttc
     2101 aagcaggttc attacaagta tggctatggt cgagacgtct tgtcggatat caatttaacc
     2161 gttccccaag ggtctaaggt ggcttttgtg gggatttcag ggtcaggtaa gacgactttg
     2221 gccaagatga tggttaattt ttacgaccca agtcaagggg agattagtct gggtagtgtc
     2281 aatctcaatc agattgataa aaaagccctg cgccagtaca tcaactatct gcctcaacag
     2341 ccctatgtct ttaacggaac gattttggag aatcttcttt tgggagccaa ggaggggacg
     2401 acacaggaag atatcttacg ggcggtcgaa ttggcagaga ttcgagagga tatcgagcgc
     2461 atgccactga attaccagac agaattgact tcggatgggg cagggatttc aggtggtcaa
     2521 cgtcagagaa tcgctttggc gcgtgctctc ttgacagatg cgccggtctt gattttggat
     2581 gaggcgacta gcagtttgga tattttgaca gagaagcgga ttgtcgataa tctcatggct
     2641 ttggacaaga ccttgatttt cattgctcac cgcttgacta ttgctgagcg gacagagaag
     2701 gtagttgtct tggatcaggg caagattgtc gaagaaggaa agcatgctga tttgcttgca
     2761 cagggtggct tttacgccca tttggtcaat agctagaaag aggagaggat gaaaccagaa
     2821 tttttagaaa gtgcggagtt ttataatcgt cgttaccata atttttccag tagtgtgatt
     2881 gtacccatgg cccttctgct cgtgttttta cttggctttg caactgttgc agaaaaggag
     2941 atgagtttgt ccactagagc tactgtcgaa cctagtcgta tccttgcaaa tatccagtca
     3001 actagcaaca atcgtattct tgtcaatcat ttggaagaaa ataagctggt taagaagggg
     3061 gatcttttgg ttcaatacca agaaggggca gagggtgtcc aagcggagtc ctatgccagt
     3121 cagttggaca tgctaaagga tcaaaaaaag caattggagt atctgcaaaa gagcctgcaa
     3181 gaaggggaga accactttcc agaggaggat aagtttggct accaagccac ctttcgcgac
     3241 tacatcagtc aagcaggcag tcttagggct agtacatcgc aacaaaatga gaccatcgcg
     3301 tcccagaatg cagcagctag ccaaacccaa tccgaaatcg gcaacctcat cagtcaaaca
     3361 gaggctaaaa ttcgcgatta ccagacagct aagtcagcta ttgaaacagg tgcttccttg
     3421 gccggtcaga atctagccta ctctctttac cagtcctaca agtctcaggg cgaggaaaat
     3481 ccccaaacta aggttcaggc agttgcacag gttgaagcac agatttctca gttagaatct
     3541 agtcttgcta cttaccgtgt ccagtatgca ggttcaggta cccagcaagc ctatgcgtca
     3601 gggttaagca gtcaattgga atcccttaaa tcccaacact tggcaaaggt tggtcaggaa
     3661 ttgacccttc tagcccagaa aatcttggag gcagagtcag gtaagaaggt acagggaaat
     3721 cttttagaca aggggaaaat tacggcaagt gaggatgggg tgcttcatct taatcctgag
     3781 accagtgatt ctagcatggt tgcagaaggt gccctactag cccaacttta tccatctttg
     3841 gaaagagaag ggaaagccaa actcacagcc tatctaagtt caaaagatgt agcaagaatc
     3901 aaggtcggtg attctgttcg ctatactacg actcatgatg ccgggaatca actttttcta
     3961 gattctacta ttacaagtat tgatgcgaca gctactaaga ctgagaaagg gaatttcttt
     4021 aaaatcgagg cggagactaa tctaacttcg gagcaggctg aaaaacttag gtacggggtg
     4081 gaaggccgct tgcagatgat tacgggcaag aaaagttatc tacgttatta tttggatcaa
     4141 tttttgaaca aagagtaatg ttcgtgtttt tagagttaaa taatttttaa actgtgagaa
     4201 agattcttct tgcagttttt tctttacatt ttttgaaaaa catctgctat ttattcggtt
     4261 aaattcttgt gttttttggt tttttgtggt aaaatgtgct caagtaatac gaaaggcgaa
     4321 ctttaaaatg tcaaaacaat tgatctattc gggaaaagct aaagatatct atacaactga
     4381 ggatgaaaat cttattattt caacttacaa ggaccaggcg actgctttca acggtgtcaa
     4441 gaaggagcag attgcaggta agggagtctt gaataatcag atctcatctt ttatttttga
     4501 gaaattaaat gcggctggtg tggcgactca ctttgtggag aaactttcag acacggaaca
     4561 actcaataaa aaggttaaga ttattccttt ggaagtcgtg ctccgcaact atactgctgg
     4621 ttccttttca aaacgttttg gtgtggatga gggaatcgcc ttggagactc cgattgtcga
     4681 attttactac aaaaatgatg atttggatga tccatttatc aatgatgagc atgtgaaatt
     4741 cctacagatt gcggatgacc agcagattgc ctacttgaag gaagaaacgc gtcgtatcaa
     4801 tgaactattg aaagtctggt ttgctgagat tgggcttaaa ttgattgact ttaagctaga
     4861 gttcggtttt gacaaggatg gcaagattat cttggcagac gaattttcac cagataactg
     4921 ccgcttgtgg gatgctgatg gcaaccacat ggataaggat gttttccgta gaggattggg
     4981 agaactaacc gacgtttatg agattgtttg ggaaaagttg caggaattga aataatctgt
     5041 ttgcaatgga aaaccttcgt ctctcaacta aaaggactca ggctgaaaag gtcccccaga
     5101 ccttttcact ctgtagagaa ctaggtgaac caaccgatgt ttacgaaatt gtctgggaaa
     5161 agttgcaggg tttaaaataa caacctcaag gctgtttggg aatattgcaa gagctgaaat
     5221 aaaggaataa gaattgatgg ataaacgtat ttttgttgaa aaaaaggctg attttcaggt
     5281 caagtcagag agtttggtta gagaactcca gcacaacttg ggactgtcaa gcttgaaaag
     5341 tattcgtatt gtgcaagtat atgatgtatt tgacttggtt gaggacttgt ttgcacctgc
     5401 agagaagcac attttctctg agcaggtaac cgaccatgtt ttagatgaag tatctgtgca
     5461 ggcggatctt gctaactatg ctttctttgc cattgaaagt ctgccagggc agtttgacca
     5521 gcgtgcagct tcgtcacagg aagccttgct tttgttggga agttcgagtg acgtgacagt
     5581 caacacagcc caactttact tggtgaataa agatattgat gcgactgagt tgaaagctgt
     5641 caaaaactac ctgctcaatc cagttgattc tcgtttcaag gatatcacga cagggattgc
     5701 caagcaggag ttttcagagt cagacaagac cattccaaaa ttgactttct ttgaaagcta
     5761 tacagcagaa gactttgctc gctacaaggc cgaacaaggg atggccatgg aagtggatga
     5821 tttgctcttt atccaagact actttaagtc aatcgggcgc gtgccaactg agactgaact
     5881 taaggttttg gacacttact ggtctgacca ctgccgtcat acgacttttg agacagagtt
     5941 gaaacacatc gacttttcag cttctaaatt tcaaaagcaa ttgcagtcaa cctatgacaa
     6001 gtatattgcc atgcgcgagg aattaggtcg gtctgaaaaa ccacaaacct tgatggatat
     6061 ggcgactatt ttcggtcgtt atgagcgtgc taatggacga ttggatgata tggaagtctc
     6121 tgacgaaatc aatgcctgct cagttgaaat tgaagtggac gttgatggtg tcaaggaacc
     6181 ttggctcctc atgtttaaaa acgaaaccca caaccatcca acagaaattg agccatttgg
     6241 tggagcggct acctgtattg gtggagctat tcgtgatccg ttgtcaggcc gttcctatgt
     6301 ttaccaagcc atgcgtattt caggtgctgg tgatattaca gcaccgattt cggaaactcg
     6361 tgctgggaaa ttgccacaac aagtcatttc taaaacagca gctcatggtt attcttcata
     6421 tggtaaccag attgggcttg ccacaaccta cgttcgtgaa tacttccacc caggctttgt
     6481 agctaaacgt atggaacttg gtgccgttgt tggtgcggct cccaagggca atgttgtccg
     6541 tgaaaaacct gaagcaggtg atgtgatcat ccttctcgga ggcaaaacag gtcgtgatgg
     6601 tgtcggtggt gcgacgggct cttctaaggt tcaaacagtt gagtctgtag agactgctgg
     6661 tgctgaggtt caaaaaggaa atgccatcga agaacgcaag attcagcgcc tcttccgtaa
     6721 tggcaatgtc actcgtctga tcaagaagtc caatgacttt ggtgcaggcg gcgtctgtgt
     6781 ggctatcggt gaattggcag acggtcttga aatcgacctc aacaaggtgc ctcttaaata
     6841 ccagggcttg aatggtacag aaattgccat ctctgaatca caagaacgga tggcggtcgt
     6901 ggttcgtcct gaagatgtgg atgccttcgt tgccgaatgt aacaaagaaa atattgatgc
     6961 tgttgtggtg gcgacagtaa ctgaaaaacc aaatcttgtc atgcactgga atggtgagac
     7021 aatcgttgac ttggagcgtc gtttccttga caccaatggt gtgcgcgtgg ttgtcgatgc
     7081 caaagttgtg gacaaggatg tcaaactccc agaagagcgt caaacatctg ctgaaacact
     7141 ggaatcagat acccttacgg ttctatctga cctcaaccat gcaagtcaaa aaggattaca
     7201 gactgtcttt gactgctctg ttggacgctc aacggttaat cacccacttg gtggtcgtta
     7261 ccaactcaca ccaactgagg catctgtgca gaaattgcca gttcaacacg gtgtgactca
     7321 tactgcgtcg gtcattgctc aaggtttcaa cccatatgta gctgaatggt ctccatacca
     7381 cggtgctgct tatgcggtta tcgaagcaac tgctcgtttg gtggctgctg gtgccaactg
     7441 gtccaaggct cgtttctctt accaagagta tttcgagcgt atggataaac aagcagagcg
     7501 tttcggtcag ccagtagctg ctcttctagg ttctattgaa gcacaaattc aacttggctt
     7561 gccatctatc ggtggtaagg actccatgtc tggtaccttt gaagaattga ccgttccgcc
     7621 aaccttggtt gcctttgggg tgacgacggc agatagccgt aaggtgctct ctccagaatt
     7681 taaagctgtt ggggaaaata tctactacat cccaggtcaa gccctctctg cagagattga
     7741 ttttgacttg attaagaaaa attttgctca gtttgaagcc atccaagctg accataaagt
     7801 gacatctgca tcagctgtca aatacggtgg tgtagttgaa agtttggctc ttgctacctt
     7861 tggaaactat attggtgcag aggtgacctt gcctgaactt aaaacagctt tgacagctca
     7921 attaggcggc tttgtcttca catctcctga agaaattgct ggagtagaga aggttggaca
     7981 aacgaaagca gactttacac tgactgtcaa cggtgtgaag ctagatggac acaagcttga
     8041 cagtgcattt caagggacat tggaagaagt ttacccaaca gaatttaccc aagcgaaaga
     8101 actagaagaa gtaccagctg tggcatcaga tgttgtgatt aaagccaaag aaaaggttga
     8161 aaaacctgtg gtttacatcc cagtctttcc aggaaccaac tcagaatatg attcagctaa
     8221 ggccttcgaa aaagaaggtg cagaggtcaa tttggtgcca ttcgtgacct tgaatgaaga
     8281 agctattgtc aagtcagttg aaactatggt tgacaatatc gacaagacta atattctctt
     8341 ctttgccggt ggattctcgg ctgcggatga accagatggt tcagctaagt ttatcgtcaa
     8401 tatcctgctt aatgaaaaag tgcgtgtggc tattgatagc tttatcgccc gtggtggctt
     8461 gattatcggt atttgtaatg gattccaagc cttagtcaaa tcgggtctcc taccctacgg
     8521 aaactttgaa gatgctaaca atactagccc aaccctcttc tacaatgatg ccaaccaaca
     8581 cgtggccaag atggtggaaa ctcgcattgc caataccaac tcaccatggt tggctggtgt
     8641 gcaagtgggc gatatccacg ctattcctgt ttcgcacggt gaagggaagt ttgtcgtgac
     8701 ggctgaggaa tttgcagagc tccgtgacaa tggacaaatt ttcagccaat acgttgactt
     8761 taacggtaaa ccaagtatgg attctaagta caatccgaat ggttctgtcc atgccatcga
     8821 aggaattacc agcaagaatg gtcaaatcat cggtaagatg ggccattcag aacgttatga
     8881 ggacggtctt tttcaaaata tcccaggcaa taaagaccag cacctgttcg cgtcagcggt
     8941 taaacatttc actggaaaat aagacttaca gattttctaa tagatagtat cagtaatgta
     9001 aaagtcatgt aaatctagct cttgatgatt acaaatgaaa attaggtata aaaaatgaca
     9061 tacgaagtaa aatctcttaa tgaagaatgt ggtgttttcg gtatttgggg acatccagat
     9121 gctgctaagt tgacctattt tggactccac agtcttcaac accgtggtca ggagggggca
     9181 ggaatcctct ccaatgatca aggacaactg aagcgccatc gtgacatggg gcttttatca
     9241 gaagttttca gaaatccagc taatttggat aaattgacag gagctggtgc gattgggcat
     9301 gtgcgttatg cgactgctgg cgaagcttct gtagataaca tccagccctt cttcttccgt
     9361 tttcacgata tgcagtttgg cttggctcat aatgggaatc tgaccaatgc agcctctctc
     9421 aagaaagaac tggaacaaag aggagcaatt ttcagcgcta cttcggactc tgaaatcttg
     9481 gctcacctca ttcgtcgtag tcataatcct agcctgatgg gcaaaatcaa ggaagcgctc
     9541 agccttgtca aaggtggttt tgcctatatc ttgctgtttg aggacaagtt gattgcggct
     9601 cttgacccaa atggattccg accgttttcg attggtaaaa tggctaatgg agcagttgtt
     9661 gtatcttctg aaacctgtgc ttttgaggtc attggtgccg agtggattcg tgatttgaag
     9721 ccaggtgaga ttgtgatcat tgatgacgag ggcattcagt atgacagcta tacagatgat
     9781 acccagttgg cggtttgttc tatggagtat atctactttg ctcgccctga ttctaatatc
     9841 cacggtgtca atgtccatac ggcacgtaag agaatgggag cacaattggc gcgagaattt
     9901 aagcatgagg cagatattgt agttggtgtg cccaattctt ccctaagcgc ggctatggga
     9961 tttgcggaag aatcaggctt accaaatgaa atgggtctga tcaaaaacca atacacccag
    10021 cgaactttta tccaaccgac tcaagaattg cgggagcaag gagtgcggat gaaactgtct
    10081 gctgtttcgg gtgttgtcaa aggcaaacgt gtggtcatgg tggatgattc cattgtacgt
    10141 ggaacaacct ctcgtcgtat cgttcagctc ttgaaagaag cgggtgcgac tgaggttcac
    10201 gttgccattg gaagtcctgc actagcgtat ccatgtttct acgggattga tatccagacc
    10261 cgtcaggagc tgattgcagc caatcatacg gtcgaagaaa ctcgccaaat cattggtgcg
    10321 gacagtctga cttatctttc aattgatggc ttgattgagt cgattggtat cgaaacagat
    10381 gcgccgaacg gtggtctctg tgtcgcttac tttgacggtg actacccaac gcctctctat
    10441 gactacgaag aagactatcg tagaagtttg gaagaaaaga ccagttttta caagtaggcg
    10501 acagattctc cattaaagaa aaggaaaaaa caaatgacaa ataaaaatgc atatgcccag
    10561 tcgggtgtgg atgttgaagc gggttatgaa gttgttgaac ggattaaaaa gcacgtggcc
    10621 cgtacggagc gtgcaggtgt catgggagct cttggtggct ttggtggtat gtttgacctt
    10681 tccaagactg gggttaaaga acccgtcttg atttcaggga ctgacggtgt cggaaccaag
    10741 ctcatgttgg ctatcaagta cgacaagcac gataccatcg ggcaggactg tgtggccatg
    10801 tgtgtcaacg acatcattgc tgcaggtgcg gaacccctct attttctcga ctacgtagcg
    10861 acagggaaga atgaaccagc taagctagaa caagtggttg ctggtgtggc agaaggttgt
    10921 gtgcaggctg gtgctgccct catcggtggg gaaacggctg aaatgccggg catgtacggc
    10981 gaagacgact atgacttggc tggttttgcg gtcggtgtgg ctgaaaaatc tcaaatcatt
    11041 gacggttcaa aggtggtaga gggagatgtt cttctcggac ttgcttcaag tgggattcac
    11101 tcaaatggtt actctttggt tcgtcgtgtc tttgcggatt acacaggtga ggaagtccta
    11161 ccagaattgg aaggcaagaa acttaaggaa gttctacttg agccgactcg tatctatatc
    11221 aaggctgtct tgccgctcat caaagaagag ttggtcaacg gcattgccca catcacaggt
    11281 ggtggcttta tcgaaaatgt ccctcgtatg tttgcagatg acctagctgc tgaaattgat
    11341 gaaagcaaag ttccagtgct tccaattttc aaagcccttg aaaaatatgg tcagattaaa
    11401 cacgaagaaa tgtttgaaat cttcaatatg ggtgtgggac ttatgttggc ggtcagccct
    11461 gaaaatgtag agcgtgtaaa agaattgttg gatgaagcag tctatgaaat tggtcgcatc
    11521 gtcaagaaag aaaacgaaag tgtcattatc aaatgaaaaa aatagcggtt tttgcctctg
    11581 gtaatggctc aaattttcag gtgattgccg aagaatttcc agtggagttt gtcttttcag
    11641 accatcgtga tgcctatgtg cttgagcgtg caaagcagct cggcgttctg tcctatgctt
    11701 ttgaactcaa ggagtttgag agcaaggcag actacgaagc agcccttgtc gaactcttgg
    11761 aagaacacca gattgacttg gtttgcctag caggctacat gaaaatcgtt ggaccaacct
    11821 tattgtcggc ttatgaaggt cggattgtca acattcatcc agcctacttg ccagaatttc
    11881 caggagctca tgggattgag gatgcttgga atgctggcgt gggtcagtct ggtgtgacca
    11941 ttcactgggt ggattcgggt gtggatacag gccaggtcat caaacaggtt cgtgtgccac
    12001 gactagctga tgataccatt gacagatttg aagctcgcat ccatgaagca gagtacaggc
    12061 tgtatccgga agtagtgaag gctctattta cagattgact ttttgatgat tcatatgata
    12121 tctttgattt taaattggag tcagtgtttg ttgaagacgg cttcaaacgg aggtatttgt
    12181 aatgttagaa tctaaaaaaa caactcgata tgtattttat gtctatctga tgttattaac
    12241 ttggggaatc ttatttaagt ttgaaacaaa tcctgaattt atagcatttt tcttagctcc
    12301 aaggtatatc aattggattc cattttcaga accactaata gtcgatggaa aaattgtttt
    12361 tgctgaaatg ttatttaatc tgatttcctt tattccatta ggtgtttgtt tccctttgat
    12421 aaaaactaat ttatctagtt taagaatagt cgggacaggt ttcttgatta gtttattgtt
    12481 tgagtgctta cagtatattt tagcaatagg tataacagat ataacggatt tgactttaaa
    12541 tacgctaggt gtctgtgtag gcttactgat ttatcaaatt tttataagag tgttcaaatc
    12601 acagactaga aaatggatca atatcttagg tatgcttagc cttggttttg cttatcttgt
    12661 tttactgtta ctgcatttaa ttggtgttta actaatgatt aaaaaggaga atataatgac
    12721 taaacgcgtc ttaatcagcg tctcagacaa agcgggcatt gttgaatttg cccaagaact
    12781 caaaaaactt ggttgggaga ttatctcaac aggtggaact aaggttgccc ttgataatgc
    12841 tggggtggat accattgcta tcgatgatgt gactggtttc ccagaaatga tggacggtcg
    12901 tgtgaagacc ctccacccaa atatccacgg agggcttctc gctcgtcgtg acttggatag
    12961 ccacttggaa gcggctaagg acaacaagat tgagctcatt gaccttgtgg tggtcaacct
    13021 ttacccattt aaggaaacta tccttaaacc agatgtgact tatgctgatg cagttgaaaa
    13081 tatcgatatt ggtgggccat ctatgcttcg ttcagcagcg aaaaatcatg ccagtgttac
    13141 agttgtggta gatcctgctg actacgctgt ggttttggac gaattggcag caaacggcga
    13201 aacctcttat gaaactcgcc aacgtttagc agccaaagta tttcgtcaca cagcggctta
    13261 tgacgccttg attgcagaat acttcacagc tcaagtgggt gaaagcaagc ctgaaaaact
    13321 cactttgact tatgacctca agcaaccaat gcgttacggt gagaatcctc aacaagacgc
    13381 ggacttttac cagaaagctt tgcctacaga ttactctatt gcttcagcca aacagctcaa
    13441 cgggaaagaa ttgtcattta ataatatccg tgatgcagat gctgctatcc gtatcatccg
    13501 tgacttcaaa gatagtccaa ccgttgtggc tctcaaacac atgaatccat gtggaattgg
    13561 tcaagctgat gacatcgaga ctgcttggga ctacgcttat gagtctgacc cagtatctat
    13621 ctttggtggg attgtcgtcc tcaaccgtga ggtggatgct gcgacagctg agaagatgca
    13681 cggcgttttc ctcgaaatca tcattgcacc aagctatacg gatgaagcgc tagctatttt
    13741 gatcaataaa aagaaaaact tgcgtatcct tgccttgcca tttaatgctc aagaggctag
    13801 cgaagtggaa gcagaataca caggtgtagt cggtggactt ctcgtgcaaa atcaagacgt
    13861 ggtcaaggca agcccagctg actggcaagt ggtgaccaaa cgtcagccaa ctgagacaga
    13921 agcgactgct cttgagttcg cttggaaggc tatcaagtac gtcaaatcaa atggtattat
    13981 cgtgaccaac gaccacatgg cacttggtgt tggtccaggt caaaccaacc gtgtggcttc
    14041 tgttcgcctt gccattgacc aagccaaaga tcgtctgaac ggggcggtcc ttgcttcaga
    14101 tgccttcttc ccatttgcgg ataacgtgga agaaatcgcc aaagcaggaa ttaaggccat
    14161 catccagccc ggtggctctg tccgtgacca agaatccatc gaagcagcgg ataaatacgg
    14221 cttgactatg gtctttacag gtgtgagaca ttttagacat taagaagata aaagggaaga
    14281 aaacagtttc tttccttttt tggcttaaaa tgctaactga aacaagatta aaacgaactt
    14341 ttttgatata atattggtaa ataattcgca aaagaggttg aggaatgaaa ctgcttgttg
    14401 tcggttcggg tggtcgtgag catgcgattg ctaaaaagtt acttgaatca aaagacgttg
    14461 aaaaagtctt tgtagctcct gggaatgatg ggatgactct ggatggtttg gaattggtaa
    14521 atatctctat ttccgaacat tctaaattga ttgacttcgc aaagaccaat gatgttgctt
    14581 ggacctttat cggtccagat gatgcccttg ctgctggtat cgtggatgat tttaaccaag
    14641 ctgggcttaa ggcctttggt ccgactaggg ctgcagcgga gctggagtgg tccaaggatt
    14701 ttgccaagga aatcatggtc aaatatggtg tttcgacagc agcctatggc acattttcag
    14761 atttcgagga agcaaaggcc tatatcgaaa agcatggtgc gcctatcgta gtcaaggcgg
    14821 atggcttggc gcttgggaag ggtgtcgtcg tttctgagac ggttgagcaa gcggtcgaag
    14881 ccgctcatga gatgcttttg gacaataaat ttggtgactc aggtgcgcgc gtggttattg
    14941 aggaattcct tgaaggagag gaattttcac tctttgcctt tgttaatggt gataagttct
    15001 acatcatgcc aacggctcag gaccataaac gtgcctacga tggcgacaaa gggcctaaca
    15061 cgggtggtat gggtgcctat gcgccagttc cacacttacc acagagtgta gttgatacag
    15121 cggttgacac cattgtcaag ccagttctag aagggatgat taaagaaggt cgcccttatc
    15181 tgggagttct ttacgcaggg cttatcctga cagctgatgg accgaaagtc attgagttca
    15241 acgctcggtt cggagatcca gaaactcaga ttatcttgcc tcgcttgacc tctgactttg
    15301 ctcaaaatat cacagatatc ctggatagca aggagccaaa tatcatgtgg acggacaagg
    15361 gtgtgactct gggtgtggtt gtcgcatcca agggctaccc gctagactat gaaaggggcg
    15421 ttgagttgcc agccaagaca gaaggcgatg tcatcaccta ctatgcaggg gctaagtttg
    15481 cggaaaatag cagagcactg ctctcaaacg gcggacgagt ttatatgctc gttaccacag
    15541 cagataccgt caaagaagcc caagccagca tctaccaaga actataccaa caaaaaatag
    15601 aaggactctt ctaccgaaca gatatcggaa gcaaggcaat taagtaaaga tataagaata
    15661 acgcgccgta gtcgccaaac acgataatgg tcgtcgtggt gaaaagacca gaacagtgaa
    15721 tgttctggtc agggggaaac ttggagaccg taggctcaaa gtttaggaat gaaaccgaag
    15781 gtttgcttcc gcctccatca cctaagacca ttatcaaaaa gaaaaataaa aattcacaaa
    15841 atacgttaat gatcgtatgg tttgcgagcg ttagcgagct aatatagaac aatcaccgcc
    15901 gttgtgaaag aacgattgga tgataatcca atcgttcagg gaaattggaa gaccttgggt
    15961 ttccaattta ggcatgagac acctttggtg gctgctgccg tccctcacaa gctaaggtga
    16021 ttgttgaaaa agaggaaaaa ggagaagaaa tgaaaccagt aatttccatc atcatgggct
    16081 caaaatccga ctgggcaacc atgcaaaaaa cagcagaagt cctagaccgc ttcggtgtag
    16141 cctacgaaaa gaaagttgtt tccgcacacc gtacaccaga cctcatgttc aaacatgcag
    16201 aagaagcccg tagtcgtggc atcaagatca tcatcgcagg tgctggtggc gcagcgcatt
    16261 tgccaggcat ggtagctgcc aaaacaaccc ttccagtcat tggtgtgcca gtcaagtctc
    16321 gtgctcttag tggagtggat tcactctatt ctatcgttca gatgccgggt ggggtgcctg
    16381 ttgcgaccat ggctatcggt gaagctggag cgactaacgc agctctcttt gccctccgtc
    16441 tcctctctgt agaagataag tccattgcgg atgcacttgc caactttgct gaagaacaag
    16501 gaaaaatcgc agaggagtcg tcaaatgagc tcatctaaaa caatcggaat tatcggtggc
    16561 ggtcaactgg gtcagatgat ggccatttct gctatctaca tgggccacaa ggttatcgcg
    16621 ctggatcctg tggcggattg cccggcctct cgcgtggcgg aaatcattgt ggcaccttat
    16681 aacgatgtgg acgccctccg tcagttggca gaccgttgcg atgtcctcac ttatgagttt
    16741 gaaaatgtcg acgctgacgg tttggatgcc gttatcaagg atggacaact ccctcaagga
    16801 acagatctgc tccgcatttc gcaaaatcgt atttttgaaa aggacttttt gtcaaacaag
    16861 gctcaagtca ctgtggcacc ctacaaggtc gtgacttcta gcctagactt ggcagatatc
    16921 gacttgtcga aaaactatgt cctcaagact gcgactggtg gctacgatgg tcatggacaa
    16981 aaggttattc gttcagaagc agacttggaa gcagcctatg cgctagcaga ctcagcagac
    17041 tgcgtcttgg aagaatttgt caactttgac cttgagattt ctgtcatcgt gtcaggaaat
    17101 ggcaaggagg tgacgttttt cccagttcag gaaaatatcc accgcaacaa tatcctgtct
    17161 aagaccatcg taccagcccg catttctgaa agtctagtag acaaggctaa agctatggca
    17221 gtgcgaatcg cagaacaact caacttgtct ggaactctct gtgtggaaat gtttgcgact
    17281 gatgatgacg ttattgtcaa tgaaatcgcc ccacgaccac ataactctgg gcactattct
    17341 attgaagcct gtgatttctc tcagtttgac acccatattc tcggtgttct cggagcacca
    17401 ttaccagtca tcaaactcca tgcgccagcc gttatgctta atgtcctcgg tcagcatgtc
    17461 gaggctgctg agcaatatgt gacagaaaat tcaagcgccc acctccacat gtatggtaaa
    17521 atagaagcga agcacaaccg caagatggga catgtgacct tgtttagtga tgtaccggat
    17581 agtgtggatg agcttgggga agggattgat ttttaggaca agtctatgat acaaattatc
    17641 gttaatacat ttattgaaaa ggataagact ggagcagttg ttgaagtgtt gtatgccagt
    17701 gctgaccaag ataaggtaca agctaaatat gaagaactag ctgcacaata ccccgaaaat
    17761 tatttagcta tctatgatgt accgctggat acggatttga atacactaga tcattacccg
    17821 tctgtgttta ttgaaaaaga ggagtttgag tagaaatctt ggtttaccta gatagcttat
    17881 tcccaacagc ttaagaagaa aggaaaaatt aacacatgat caaccgttac tctcgccctg
    17941 agatggcgaa tatttggagt gaagaaaata aataccgtgc ttggcttgag gtggaaatcc
    18001 tctctgacga ggcatgggct gagttggggg aaatccctaa ggaagatgtg gctttgattc
    18061 gcaagaaggc ggactttgac atcgaccgta ttttggaaat tgagcaggag acgcgccacg
    18121 atgtggtggc tttcacgcgt gcggtttctg agactcttgg tgaagagcgc aagtgggttc
    18181 actatgggtt aacttctacc gacgtggtgg atactgctta tggttacctc tacaagcagg
    18241 ccaacgacat catccgtcgt gaccttgaaa acttcactaa tatcatcgct gacaaggcca
    18301 aggagcacaa gttcaccatc atgatggggc gtactcatgg tgtgcacgct gagccgacaa
    18361 cctttggtct taaattggca acttggtaca gcgaaatgaa acgcaatatc gagcgtttcg
    18421 agcatgcagc tgctggtgta gaagctggta agatttctgg tgcggttggg aactttgcca
    18481 atatcccacc atttgtagag gagtatgtct gcgataaact tggcatccgt gcccaagaaa
    18541 tctctacaca agtccttcct cgtgaccttc acgctgagta ctttgcggtt cttgccagca
    18601 ttgcgacttc aatcgaacgt atggcgactg agattcgtgg tctacaaaaa tctgagcaac
    18661 gcgaagtaga agagttcttt gctaaagggc aaaaagggtc ttcagcaatg cctcacaaac
    18721 gcaacccaat cggttctgaa aatatgactg gtctggcgcg tgtcattcgt ggtcacatga
    18781 ttacggctta tgaaaacgtc gctctctggc acgaacgcga tatttctcac tcatcagctg
    18841 agcgtatcat cacaccagat acgaccattt tgattgacta catgctcaac cgttttggaa
    18901 atatcgtcaa gaacttgaca gtcttcccag aaaatatgat ccgaaacatg aactcgactt
    18961 ttggtcttat ctttagccaa cgggctatgt tgacattgat tgaaaaaggc atgacccgtg
    19021 agcaagccta tgacttggtg caaccaaaaa cagcctactc ttgggacaac caagtagact
    19081 ttaaaccact tcttgaggca gattcagaag taacatcacg tctcactcaa gaagaaatcg
    19141 atgaaatctt caacccagtt tattacacca aacgagtgga tgatatcttt gaacgtcttg
    19201 gactaggtga ttaattaaaa aataaacagc gagcttcaat ctcgctgttt attttttatc
    19261 gaaaagactt agtcttcttt tcttttagtg agtccatagg ctgctagtgt ggacatgagt
    19321 cctgcgacta ctagtcctgc agaatcgtga gttcctgttt caggaagttt tttctctgtt
    19381 accacaggag ctggatcttg aggaagagct ttgctttcct cagcgggagc agttgatgga
    19441 gctggttggc ttgggatttc tagttttggt ttttcttcag caatagcggc ttgtccgttt
    19501 tcatcgccta catgtgttac catagttccg acttcgacta tttgagtaac ggcttcctgt
    19561 gctacgacac tatttacaag tgttttcact tccttaccat cggcagaagt gctcacagag
    19621 tagaagttgc tacgatgtcc attgacgccc ttagtaatga cttgtgtttt tcctttgagt
    19681 aagagtggat tttcacgagt cactgtggta aatggaattt cttctttttg gatatccagt
    19741 ctaggtttta cctcagtagt tggtgcaaga ccactttcat cacccttgtg agttacagga
    19801 gcgccaactt caaccacttg gtttataact tctttggtta cctggctatc aaggactgtt
    19861 tctgttgttt ttccattttc agtgagtaca gagatgtaat gagttcgttc acctttgact
    19921 cctgctgtga taatattttc ctgaccagct gggaggttag gattttcttt cttgataact
    19981 tcaaatggaa tttcttcagt tcttgtgatg agttctggtc tggtttcaac attggcagcc
    20041 acttcatttt catccaggct tcctgaatga gttgcagctg gtttgaggcc ttgaagagcg
    20101 gcttttaggt tggctacaag tgtgtcaagc tcagcttgtt tattacggtt gaggttgtaa
    20161 tttagagctg ttttagctgc gtcaagggcc tcaagacttt ctttactata tccttctaag
    20221 tttgtaggaa ttttagctaa ttcttcgcgg agagcattat aattagcacg gaagtagtct
    20281 ttgttgtggt ctgcaaaggc agtcatgagt tcaaagattt cctcttcctt gtactcagcg
    20341 cttggtctat ctgcccagat tgaaagcata cttccgactg ttggaagatc tacttcagga
    20401 tatttggtag aagctagttg attgaatggt gtttttccag tattctcaat agctttcttg
    20461 aggaaaccac caccatcttc tggtttttga ccaagaatgt agtaccagtc accgttggta
    20521 ttcaagaatt tatagccttt gcttgctagg tattgaggtg atgcgaggtt atatccccac
    20581 cagcctttag accagtaaga aatcaagaca tctttgtcaa actgaacatc gtccttgtct
    20641 tcatagtaga agccatcgtt gaaggccatt ggttgaagcc ctctttcttt ggccatagct
    20701 gcgagggtgt tggcatattc ggcaaatttg ccatagagtt gataccactt gaggtagtac
    20761 cagccttggg cactagtcgc atcgttggcg tattcgtcag taccaaagtt gaaaatcttt
    20821 gttttacctg caaagaagtc catgtattta ccgatgaggg cttttacaaa gttcatcgct
    20881 tcttcgtttt tcaagtccat agttgttttt gaaactttat caaagtgggc ttgaggattt
    20941 ttaataccta atttttccat ggcaaccagc atagcatcca tgtgacctgg actgttaata
    21001 gctgggatga gaccgatgtc cttagattta gcgtattcaa ttagctctgt tacttctgcc
    21061 tgtgttagtg cagtaccgtt tggatcgtcg tagtaagctt tagttccttc gataatagct
    21121 tttttaacgt catcactagc ataggttttt ccgttggcag taatggtcat atcatcgagt
    21181 agaaagcgaa gtccgtcatt tcctagaagg agatggacat cagaatatcc gagctcactg
    21241 gccttgtcta cgatgcgttt gagctggttc agagtaaagt atttgcgtcc agcatcgatt
    21301 gagattacct tgtttttggc aagtttttca acctcacgtt tagcttcttc ttcttttaga
    21361 gcttcaggcg tgagggtcaa gttgttgaca gtttcttgaa gtttagcaat ggcttgatca
    21421 atcgtatctt gttgggcacg gctaaggttg ctatcgagag agcgaatagc tttttcagct
    21481 tcttttacgg ccgtgacgct ttctgcagta taacggttca ggtcttttgg tacctcgtta
    21541 agtgcttgct ctgcagattc ataatcagct gcgaagtatt cagcgttggc atttgcaaaa
    21601 tgacgcatga gtttgaagag gcgtgatggt gaataacgtg cagatggagt gtcagcccaa
    21661 gcagctacca taccaccgat gattgggata tcagctcctt ctgtttttgg tacagaagtg
    21721 attggtgtgt ttttaatacc attgagcccc tgatcgagat tgtaccagcc ttggccatca
    21781 gcgtttcgtc caagaacgta gtaccaagca tcattggtat taaggatttg gtgacctttt
    21841 tcagctagta gtttagaaga agcgacatcg tagcctcccc aaccaccagt ccacatagaa
    21901 acgatgatgt ctttgtcaaa actaccaaag cttgtgtcgc tattgtagta gataccgtcg
    21961 ttaaaagcca ttggtttgag accgtgcgat tttacaatac gagcgaggtc attggcgtag
    22021 gcaataaatt tttcatagcc ttttacaggg tagccttcgt ttggatagta tttatcagct
    22081 tgaagcacac tccaaccttt agcatctgtc gcatcattgg catattcatc aagtccgatg
    22141 ttgaagattt cagtcttttt cgcgaaataa gcagcatact tgtcgataag ggcttttgta
    22201 aaagcgacag cttgttcgtt gtcaagatcg acagtacggg ctgatttctt cccaaaatag
    22261 ctaaagttag ggttttggat tcccaattct ttcatggcat tgagaatcgc atccatgtgt
    22321 ccaggactat ttactgtcgg aatgagaccg atacctttat ctttggcata gttaatcaga
    22381 tctgtcattt gactttctgt taagtgattg ccgtttggat cgttgtaata atcatttgta
    22441 cctttttcaa tggcgcgttt gacatcgtca ctggcatagg tcttgccgtt agctgtgatg
    22501 ctcatatcgt ccaacatgaa acggagtcca tcatttccga ctaataggtg taaatcagtg
    22561 tagccataat gtttcgcttt atcgatgatt tccttgagtt gttctggtga gaaatattta
    22621 cgtccagcat caatagaaac aattttcttt ttcgctagtt tttcatttac agttgcagca
    22681 cgttcctttc ctgcctctgt ttccggtttg tcagcctctg ctttcgcttc atctttttta
    22741 gctggtttat ccttgtcagt cttgtctgta tttgactctt tagaatcaac ctctttcgct
    22801 tcttcctttt tagggctagc ttcttctgcc tttttattag cagtttcttt ttcagcagaa
    22861 gttggagtta ccacttctgc tttatcacta ggagttgaac taacttcctc ttgtggtttt
    22921 tcttctgttt ttggaagact agctacctta tcagtagctg gagtttctgt ttctacagtt
    22981 tttggagctt ctggttgaag cactgcttta ggtgtttcct cagtccgatt ttcggatgat
    23041 tgaggggaat cagaaaccgt atggatggtc ggttggtttt ctgtagtagt aggagtaact
    23101 ccatcggctg caacagtctg tgcttggaag gcaaatccaa ttagaacaga agctgctcct
    23161 acagcgtatt tacgaataga aaaacgctgt tgtttttcat gtttcattgc aaaacctcct
    23221 gattgcattg ttatattgat agcgattata taaatcaacg cctttatttt atttcttgtt
    23281 ttaacgagag tcaagaggag atgacaaaaa actataataa gtataaaaaa atataaaatt
    23341 taaacttaag atttcagatt ggtcggaaaa aatacgtata tatatctagt ataatttttg
    23401 gttctatttc tataaaatat tccacaaatt atagaatttt ccaaaaatag gtaagcgcta
    23461 cctttttggt gtggtataat aagcatagaa aaagcccaag cgattagctc aggctttctt
    23521 cttagtgttc acggtcacat gagataaatt taatcttgta gtaatcagat cgtttgtaag
    23581 tttcactgta ttctaaaact tggccagttg attcgagttt ggtgatttta gtttgtagga
    23641 cagtagggaa ttgttcatcg actccgagga ctgaagccgc atgttctgga gttggaaaga
    23701 ctatttcgtt gatttcttca aagtgttcat cattcatgtg aatgtggtag tctaacttga
    23761 aacgattata gatagaacta tagtattcaa ggtttggata atttgcgttg atatattgtt
    23821 ctgggatgta ggatgtatgg tagatataaa cgacaccgtt tgattcgcgg atacgttcaa
    23881 tcttgtagta gaattgatcg ccacgtagac ccaatttttc caagtaaaca agcttgtttc
    23941 cgcgttcaat tgaaagaaca gttaccttat catctttagc attgaagagt tcaatatctg
    24001 aaaactctac aagcttgtgt ttgcgtgcac gtgaaacgaa agttcctttt ccttgttggc
    24061 ggacaatata gccatctttg gcaaggtcgt ttaaggcgcg aacaactgtg atagagctga
    24121 catcgtacat tgaaatgagt tctgcttcag tgtaaaattt atctccactg ctaaactgcc
    24181 cagagatgat tttatttttt aattcgtctt ttatgtattg atatttggga attgccatat
    24241 ttttacctct attttccttc tccatagaaa attttaccat aaaagcgaaa aaaatagtag
    24301 tcttttgaat aaaaaattag aataatagtt tgaaaaatta agcgatatag ggattgctca
    24361 tattttataa atgcattcta tacaaaatta ttttaggtac tatattccaa atttaaagag
    24421 cgtttgaaat gggtctaaaa atgtttgaaa ctaattgaat gtgtgtataa tagtaaaaat
    24481 gcagtaaata tcccgacaat ttctattgac aattcaaaca gattggttta taattaatat
    24541 aacaacaaat gaaagcgcaa actttctcgg tcggaaggta gttttatgac acgatttgag
    24601 atacgagatg atttctatct cgatggaaaa tcatttaaga ttttatctgg tgccattcat
    24661 tattttaggg ttcctccaga ggattggtat cattcgctct ataacttgaa ggctcttggt
    24721 tttaatacgg tagagactta tgttgcttgg aatttacacg agcctcgtga aggtgagttt
    24781 cattttgaag gtgatctgga tttagagaaa tttctccaaa tagcgcagga tttgggtctc
    24841 tacgcaattg tgcgtccgtc tccatttatc tgtgcggaat gggaattcgg tggcttacca
    24901 gcttggctct tgaccaagaa catgcgaatt cgctcatccg acccagcata tatcgaggca
    24961 gttggtcgct actatgatca gttattgcca agactggtgc ctcgtttgtt ggacaatggt
    25021 ggcaatattc tcatgatgca ggttgaaaat gagtatggtt cttacggaga agataaggct
    25081 tacctgagag cgattcgaca gctaatggaa gagtgtggcg taacctgtcc cctctttaca
    25141 tcagatggtc catggcgagc tactctgaaa gctggaacct taattgaaga ggacctcttt
    25201 gtaacaggaa actttggttc taaggcacct tacaactttt cgcagatgca ggaattcttt
    25261 gatgaacatg gtaagaaatg gccactcatg tgtatggagt tctgggatgg ttggttcaat
    25321 cgctggaaag aaccgattat cacacgggat cctaaggaat tggcagatgc agttcgagag
    25381 gttttggaac aaggctctat caatctttac atgttccacg gtggtacaaa ctttggtttc
    25441 atgaatggtt gctcagctcg aggaactttg gacctgccac aagttacgtc ttatgattac
    25501 gatgcccttc tggatgaaga aggaaatcca actgctaaat atcttgcagt caagaagatg
    25561 atggcaacac atttttcaga gtatccgcag ttggaaccac tctacaaaga gagtatggag
    25621 ttggatgcta ttccactagt tgaaaaagtt tctttgtttg aaaccttaga tagcttgtca
    25681 agtcctgtag aaagtctcta tcctcaaaag atggaggagc tgggacaaag ttatggctac
    25741 ctactttatc gaacagaaac aaactgggat gcagaagaag aaagacttcg tatcattgat
    25801 ggtcgagata gggcccagct gtatgtcgat ggtcagtggg ttaaaactca atatcagaca
    25861 gagattgggg aagatatttt ttatcaaggt aaaaagaaag ggctatctag gttagatatc
    25921 ttgatagaaa atatggggcg tgtcaactat gggcataagt tcttagcgga tacgcaacgt
    25981 aagggaattc ggacaggggt ctgtaaggat ctgcatttct tactaaactg gaaacactat
    26041 ccactcccac tagacaatcc tgagaaaatt gatttttcaa aaggatggac tcaaggacaa
    26101 ccagcctttt acgcttatga ctttacagtc gaagagccaa aagatactta cctagacttg
    26161 tctgagtttg gtaagggggt tgcctttgtc aatgggcaga atctaggacg tttttggaac
    26221 gttggcccaa ctctctcact ttatatccct catagctatc tcaaggaagg tgccaaccgc
    26281 atcattatct ttgaaacaga aggtcaatat aaagaagaga ttcatttaac tcgtaaacct
    26341 acactaaaac atataaaggg ggaaaactta tgacaattgt aggatgccgt atcgatggac
    26401 gtttgatcca cggacaagta gccaatcttt gggctggaaa actaaatgtt tcacgcatta
    26461 tggttgtaga cgacgaagtt gtcaacaacg atattgaaaa gagtggtttg aaacttgcga
    26521 caccaccagg tgtgaaattg agtattttgc caattgaaaa agcggcagcc aatattcttg
    26581 ctggtaagta cgatagccaa cgtctcttta tcgtggctcg taaaccagac cgcttccttg
    26641 gcttggtcga agcaggtgta ccacttgaaa ccctcaatgt tgggaatatg tctcaaacac
    26701 cagaaactcg ttctattaca cgttctatca acgtagtaga caaggatgtg gaagatttcc
    26761 acaaactggc agaaaaaggt gttaaactta ctgctcaaat ggttccaaat gatccaattt
    26821 cagacttttt gagcttatta aaataggaaa aaatttttag gaggtcattg ttatgataca
    26881 atggtggcaa attttacttc tcactttgta ctcagcttat caaatctgtg atgagttgac
    26941 gatcgtttca tctgcaggtt cccctgtatt tgctggtttc attactggtt taatcatggg
    27001 agatgtgact actggtttgc ttatcggtgg taacttgcaa ctgttcgttc ttggggttgg
    27061 taccttcggt ggtgcttctc gtatcgacgc aacttctggt gcagttcttg cgacagcctt
    27121 ctctgtttca caaggaattg atgcaccgct tgcaattact acaatcgctg taccagtagc
    27181 agctctcttg acttacttcg acgttcttgg acgtatgact actaccttct tcgctcaccg
    27241 tgtggatgct gcaatcgaac gctatgacta taaaggtatt gaacgcaact acttgcttgg
    27301 tgcgattccg tgggctctat ctcgtgccct tccagtcttc tttgcccttg cttttggtgg
    27361 tgcctttgta caacatgtag tagacttagt gacagaatac caatggattg cagatggttt
    27421 gacactcgca ggacgtatgc ttccaggtct tggatttgca atcttgcttc gttaccttcc
    27481 agttaaacgt aaccttcact accttgctat gggatttggt ttgacagcta tgttgactgt
    27541 tctttactca tatgtaacag gtcttggtgg cgctgttgct ggtatcgtag gtactcttcc
    27601 tgctgaagtt gctgaaaaaa ttggtttcgt gaacaacttc aaaggtttgt ctatgattgg
    27661 tatttctatc gtaggtattt tccttgcagt gcttcacttc aaaaatagcc aaaaagtagc
    27721 tgtagcagca ccttctacac catcagaaag tggggaaatc gaagatgacg aattctaatt
    27781 acaaactaac aaaagaagat tttaatcaaa tcaacaaacg tagcttgttt actttccaat
    27841 taggttggaa ctacgaacgt atgcaagctt ctggttacct ttacatgatc ttgcctcaat
    27901 tgcgtaaaat gtatggggat ggaactcctg aattgaaaga aatgatgaaa gttcatactc
    27961 aattcttcaa tacttcacca ttcttccata ccattatcgc tggttttgac cttgccatgg
    28021 aagaaaaaga tggtgtgggt tcaaaagacg ccgttaatgg tatcaagaca ggtttgatgg
    28081 ggccattcgc tcctcttgga gatacaatct ttggttcact tgtacctgcc atcatggggt
    28141 cagtcgcagc aactatggct atcgctggcc aaccttgggg tatcttcctt tggattgcag
    28201 ttgcagttgc ttatgacatc ttccgttgga aacagttgga atttgcttac aaagaagggg
    28261 ttaaccttat caacaacatg caaagtacct tgacagcttt gattgacgct gcatctgtac
    28321 ttggtgtctt catgatgggt gctcttgtag caacaatgat caactttgaa atttcttaca
    28381 agttgccaat cggtgaaaag atgattgatt tccaagacat cttgaaccaa atcttcccac
    28441 gtttgcttcc agcaatcttt actgccttta tcttctggtt gcttggtaag aaaggtatga
    28501 actctactaa agctatcggt attattatcg tacttgcttt ggctctttct gcccttggtc
    28561 actttgcatt tggaatgtaa ttccttatga ctaaatcatt aattttggtg agccatggtc
    28621 gcttctgtga ggagcttaaa ggtagcacag aaatgatcat gggcccacaa gacaatattt
    28681 acacagtagc tcttcttcca gaagatggcc cagaagactt tactgcaaaa tttgaagctg
    28741 ctattgaagg attggatgat ttcctagtct ttgcggatct tctcggtggg acaccttgta
    28801 acgtggtcag tcgtttgatc atggaaggtc gtgacatcga actttatgca gggatgaatc
    28861 tcccaatggt gattgaattt atcaatgcga gcctgacagg cgcagatgcg gactacaaga
    28921 gccgtgctgc agaaagcatt gtgaaagtta atgacctgtt agcaggcttc gatgatgacg
    28981 aagatgaata agatgtgaca acgtgaacat cgtcagaaca acttatatag aatatacatg
    29041 ggaatggggc ttactcccat tcccatattt aatagaaaaa gagggaatca atgctacatt
    29101 atacaaaaga agacttgatt gaattgggtg cagaaatcac gacacgtgaa atctaccaac
    29161 agcctgatgt atggaaagaa gcttttgaat cttatcaaga aaaacgtgaa gaaattgcag
    29221 ccttcctaca agggattgcg gataaacatg actatatcaa ggttattttg acaggtgctg
    29281 ggacttctgc ttatgtggga gataccttgg tgccttactt taaggaagtc tatgacgaac
    29341 gcaaatggaa tttcaatgct attgcgacaa ctgatatcgt tgccaatcca gaaacttatt
    29401 tgaaaaaaga tgtggcaact gtccttgtgt cttttgctcg tagtgggaac tcgcctgaaa
    29461 gtgtggcgac tgttgattta gcaaaagcct tggtggatga gctttaccaa gtgacgatta
    29521 cttgtgcagc agatggtaaa ttggctcttc aagcccacgg cgatgaccgt aatctattgc
    29581 tcttgcaacc agctgcttca aatgacgctg gatttgccat gacttctagc tttacgtcta
    29641 tgatgttgac agcactcttg gtctttgatc ctacagaatt tgctgtgaaa gctgaacgtt
    29701 ttgaagttgt atctagtctt gctcgtaaag ttttagataa tgcagaagat gtcaaagagc
    29761 ttgttgacct agactttaat cgtgtcatct atctaggcgc tggtccttac ttcggccttt
    29821 ctcatgaagc gcaacttaag attttagaat tgacagctgg tcaagttgca actatgtatg
    29881 aaagccctgt tggcttccgt cacggtccaa aatcattgat caacgaagat acagtcgttt
    29941 tagtttttgg tacaacgaca gattatactc gtaagtatga tttggatttg gttcgtgaag
    30001 ttgcaggtga tcagattgct cgtcgtgttg tgcttttgag tgatcaagct tttggtcttg
    30061 aaaatgtcaa agaagtggca cttggttgtg gcggtgtctt gaatgatatt taccgtgtct
    30121 tcccttacat cgtttatgcc caactctttg ctttattgac ttcactcaag gtagaaaata
    30181 aaccagatac accgtctcct acaggtacag taaaccgtgt agtacaaggt gtcataattc
    30241 acgaatatca aaagtaagac agtgtttatg aattcttgac aagaggattt gtaaattatc
    30301 agataaacca tagattgtca gtacgctttc tatggtttgt ttgcttgaga gaaatagtaa
    30361 aaggagaaca gaatgaaagc atacacagag cgtgtatttg gaaatgttga gggtgaggat
    30421 gtcttggcct atcgatttga gacagacggt ggctaccaac ttgaggttat gacttatggt
    30481 gcgactatct tgcgctatgt cgcacctgac aaggctggaa attttgccaa tgttatcttg
    30541 ggatttgatg actttgatag ttatgtaggc aatagtccca agcatggagc aagtgtaggt
    30601 cctgtagcgg gtcgtattgc aggtgcgacc tttgagctca atggtaagac ctatgacctt
    30661 gaggttaata atgctagcaa ctgtaatcac agtggttcaa ctggttggga ttccagcttg
    30721 tttgaagttg aagaagtaag cgatcatggc ttgactctct acacagagcg tacagatggg
    30781 acaggagggt tccctggaaa tctcaagatt tggatcagtt atcacttgga agaaactggt
    30841 gcctatgaaa tcagctacaa ggtaacgacc gatcaggata cgctggtcaa tccaaccaac
    30901 cacagctatt tcaacttgtc tggtgatttc acgcagacga ttgaccgtca tgtcttccaa
    30961 ctaaacacag agggcattta ctcaatcgct cctgacggtg ttcctgccaa aactccagaa
    31021 gccaaccgtg atgtggtcaa acacgtctac aatggtacct tgttgaagga tatctttgca
    31081 gaagaagatg agcaaatcca gctggcatca ggtttggatc atccatttgc ccttcctgca
    31141 ggccatgaca atgctggatt cctttatgac caaaattcag gtcgcttcct gcttttcaag
    31201 acagaagctc cttgctttgt ggtctacaca gcaaactttg tggatgaaag tgtcatcata
    31261 ggaggtcagc caatgctaca gcacaatggg attgctcttg aagcgcaagc tttaccagat
    31321 gccattcaca gtgaccttaa aggccaagtc attcttaaag ctggtcaaac cttcaccagt
    31381 aagacacgtt atgaacttgt tgtgaaataa aaaaagctgg tcaaaaagtc tttaaaatta
    31441 aaataacgca tattgtcagg tgatgaaaag tttgataata tgcgttttat tatgaaatga
    31501 tttactttat attatcctga aatggtgtta tttttgagtt atctggcttg actttcttga
    31561 tggaaattat ataatagttg taacaataat aattacaact agaaaagagg accatttatg
    31621 acctatgaat acaagagtca catttatttg gcagagacag ctttaaatgt aaaggatttg
    31681 gccagtcaaa cagcctttta tcagcaagtt attggtttag aaatcttatc tcaaactgag
    31741 acagagtcca ttctaggcct tggtggaaaa gtcttggtac agctgattca agcacaagag
    31801 agcggagaag tgagggaata ttatggtctt taccatttgg ccattctctt gcccacacgc
    31861 aaggctttag ctgatgtctt gaaacacctg acagatttac aaattcctct tgtcggcggt
    31921 gcagaccatg gttacagtga agctatttac ttggaagatt tggagggaaa tggcattgaa
    31981 ctctatcgag ataagccagt ttccacatgg gatattcgag aagatggacg cattatcgga
    32041 gtgacggagg ctcttgcggc gcaggacatt tatgagttgg gggaaagagt agagcccttt
    32101 atcctggcag aaggtacaag aatggggcat attcatcttt ccgtcaagga tagtcgaaag
    32161 tccagccagt tttatcaaaa ggtgttaggg ctagagaata aattcagtgt gcctggcgct
    32221 agttggattg cggctggaga ttaccatcat catttagcag ttaacgaatg gggaggaaaa
    32281 ggtctggctt cgcgtaagca aggcttacca ggtttagcct actatgtcat cgaagtcgca
    32341 cataaaaaag aactgttaac gattgcccaa cgagcacaag aagttgacgc accaatcaaa
    32401 tggatgacat cgatccaatt ggaaatcaca gactcagatg gcatcgtgac ccgtattcgt
    32461 ttagcaaggt aaaattagga atttttaata agttataagt attgctagtt tgctttttga
    32521 acggtctttg gtaacagaac tgcgtgagaa ttttgacaaa gaagctgatt agagatataa
    32581 tgaagaaaaa tcgagcaagg aaaaagaact gacaaattca aagtatatta cttgtttgaa
    32641 acgttcagag ggccagttgt gtgggattca aaagatgatt gaaggagatc gtgactgtgc
    32701 tgatattgtg acgcaactga cagcagtgaa atctagtgta gagcgcgtga ttgagatgat
    32761 aattaccgaa aatcttactg aatgtatcaa tcagccccta gatgattctg aagctcaaaa
    32821 ggaacgccta gaaaaggcta tccgatactt gattaaacgg aaataaatga atgttttgag
    32881 acgatatgtg caaaggtcta ttaactatta gaatagttta atgtgagtca aaagaagttg
    32941 taaaaaatct ctaaaattaa aaaaacgcat agtatcaggt gttgaatgta ctgaccccca
    33001 aaagttagat tttttctgtc taacttttgg ggtgcagttc ataagaacct tggtaatatg
    33061 cgttttatta tgtagagact tatactcttc gaaaatctct tcaaaccacg tcagcttcac
    33121 cttgccgtag atatggttac tgacttcgtc agttctatcc acaacctcaa aacagtgttt
    33181 tgagctgact tcgtcagttc catccacaac cttaaaacag tgttttgagc agcctgcggc
    33241 taacttccta gtttgcactt tgattttcat tgagtattac ttcatctttt cctgaaattg
    33301 agtttttgcc caactcctct tgattattct acttgaccgg gccaagcatt cataccacct
    33361 tctacgttgg taacggtgag gccttgggag ctgagaaact ggcaggtaga tgcagagcgg
    33421 actcctcctt gacaaatgac atagtattca tggtcaggtt tgagttcttt gtagccttgc
    33481 tctaaggtac ttaatggaag atttttggca cctggtgcat gtcctgcttg gaattcatgt
    33541 gcttcacgga catcgataag ttctagattt tcattttggt attttccata aaagtcagcc
    33601 atgctgatac tagtttccat attctactcc ttctgggtta accattttgt atagtgaata
    33661 agcgccgtca aggttttggg cggtaaatcc tgcttgcttg aggataggct ctgcgatata
    33721 gctgcgcaaa ccactgtggc agctgacgat gtaggcttgt tttttgtcca gttcatcagt
    33781 ttcctccttg acactttgtc agtttttgat acaatagtac aaaattagag gaggcaggct
    33841 atgattcaga aacatgcgat tcctatttta gagtttgatg acaatcctca ggcggttatc
    33901 atgcccaatc acgaggggct ggacttgcag ttgccaaaga agtgtgttta tgcattttta
    33961 ggtgaggaga ttgaccgcta tgcaagggaa gtaggggcgg attgtgtcgg cgaattcgtt
    34021 tctgctacca agacctatcc agtctatgtc atcaactaca agggtgagga ggtctgtctg
    34081 gctcaggctc ctgttggctc cgctccagca gcccagttta tggattggtt gattggctat
    34141 ggtgtggagc agattatctc tactgggacc tgtggtgtcc tagctgatat agaggaaaat
    34201 gcctttctag tccctgttcg cgctttgcga gatgagggag ccagttacca ctatgtggca
    34261 ccttgtcgtt atatggaaat gcagccagag gctattgctg ctattgagga agttttggaa
    34321 gacagaggga ttccttatga agaagtcatg acctggacga cagacggttt ttaccgagaa
    34381 acggctgaaa aggtggctta tcgtaaggaa gaaggctgtg ctgttgtgga gatggagtgt
    34441 tctgctcttg cggcagtagc tcaattgcgt ggggttctct ggggtgaatt gttgttcaca
    34501 gcagattctc tagcggactt ggaccagtac gacagtcgtg actggggctc ggaagctttt
    34561 aataaggcgc tagaactgag tttagcaagt gttcaccacc tttagttgta ctggcaaagg
    34621 atttgtttta tcataaaatg tctagctcat acttttcaaa aatatgttta aacgaggtca
    34681 ccttcctctt gtcctaggca tgttgaggtt gggaaaaatc tttaaaatca gaaaaacgta
    34741 tcatatcagg tgatgaaaat tttgatacta tgcgttttat gttggtaaga tttagagtga
    34801 gatgaaatga tactcttcga aaatctcttc aaaccaggtc agcttcacct tgccgtatag
    34861 atgttactga cttcgtcagt tctatctgca acctcaaaac agtgttttga gctgacttcg
    34921 tcagttctat ccacaacctc aaaacagtgt tttgagcaac ctgtgactag ctttctaagc
    34981 gatgccttgg ttctcattgc ctataatcaa aaagagaaat tttctcctga aaagcatata
    35041 gagtagctgg cgttaaaagc tcctgtcttg cttttttgac ctatagtcac atctatcaag
    35101 tattgttctt gcctaagcta tcaataaaaa ggtggcattt tttaggcttg gtgttagtga
    35161 attttgcctt atcctatcta agtcatttcg agctttttat ggtacaatgg aaacatgtta
    35221 ttcaaattat ctaaggaaaa aatagagcta ggcttatctc gtttatcgcc agcccgtcgt
    35281 atttttttga gttttgcctt ggtcatttta ctaggctctc ttcttttgag cttgcccttt
    35341 gtccaagttg aaagctcacg agcgacttat tttgatcatc ttttcactgc tgtctctgca
    35401 gtctgtgtga caggtctctc aacccttcca gtagctcaca cctataatat atggggccaa
    35461 ataatctgtt tgctcttgat tcagatcggt ggtctagggc tcatgacctt tattggggtt
    35521 ttctatatcc agagcaagca aaagcttagt cttcgtagcc gtgcaactat tcaggatagt
    35581 tttagttatg gagaaactcg atctttgaga aagtttgtct attctatttt tctcacgacc
    35641 tttttggttg agagcttggg agctattttg cttagttttc gccttattcc tcaacttggc
    35701 tggggacgtg gtctttttag ttccattttt ctagcgatct cagccttctg taatgccggt
    35761 tttgataatt tagggagcac cagtttattt gcttttcaga ccgatttact ggtcaatctg
    35821 gtgattgcag gcttgattat tacaggcggc cttggtttta tggtctggtt tgatttggct
    35881 ggtcatgtag gaagaaagaa aaaaggacgt ctgcactttc atacgaagct tgtactattg
    35941 ttgactatag gtttgttgtt atttggaacg gcaactactc tctttcttga gtggaacaat
    36001 gctggaacga ttggcaatct ccctgttgcc gataaggttt tagttagctt ttttcaaaca
    36061 gtgacgatgc gaacagctgg cttttctacg atagattata ctcaggctca tcctgtgact
    36121 cttttgattt atatcttaca gatgtttcta ggtggggcac ctggaggaac agctggggga
    36181 ctcaagatta cgacattttt tgtcctcttg gtctttgcac gaagtgagct tctaggcttg
    36241 cctcatgcca atgttgcgag acgaacgatt gcgccgcgaa cggttcaaaa atcctttagt
    36301 gtctttatta tctttttgat gagcttcttg ataggattga ttctgctagg gataacagcc
    36361 aaaggcaatc ctccctttat ccacctcata tttgaaacca tttcagctct tagtacagtt
    36421 ggtgtaacgg caaatctgac tcctgacctt gggaaattgg ctctcagtgt tatcatgcca
    36481 cttatgttta tgggacgaat tggtcccttg accttgtttg ttagcttggc agattaccat
    36541 ccagaaaaga aagatatgat tcactatatg aaagcagata ttagtattgg ttaagaaagg
    36601 aaagagcatg tcagatcgta cgattggaat tttgggcttg ggaatttttg ggagcagtgt
    36661 cctagctgcc ctagccaagc aggatatgaa tattatcgct attgatgacc acgcagagcg
    36721 catcaatcag tttgagccag ttttggcgcg tggagtgatt ggtgacatca cagatgaaga
    36781 attattgaga tcagcaggga ttgatacctg cgataccgtt gtagtcgcga caggtgaaaa
    36841 tctggagtcg agtgtgcttg cggttatgca ctgtaagagt ttgggggtac cgactgttat
    36901 tgctaaggtc aaaagtcaga ccgctaagaa agtgctagaa aagattggag ctgactcggt
    36961 tatctcgcca gagtatgaaa tgggacagtc tctagcacag accattcttt tccataatag
    37021 tgttgatgtc tttcagttgg ataaaaatgt gtctatcgtg gagatgaaaa ttcctcagtc
    37081 ttgggcaggt caaagtctga gtaaattaga cctccgtggc aaatacaatc tgaatatttt
    37141 gggtttccga gagcaggaaa attccccatt ggatgttgaa tttggaccag atgacctctt
    37201 gaaagcagat acctatattt tggcagtcat caacaaccag tatttggata ccctagtagc
    37261 attgaattcg taaagaggga tgacccctct tttttgatgc ccaagatggc aaatagagac
    37321 agaagcccct tgtcttctag taaaagttct tcaaaggctg gactttatgg taaaatagaa
    37381 ggaagtgaca agagagagta atactcaatg aaaatcaaag atcaaactag gaaactagct
    37441 gcgggctgct caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagctcaaaa
    37501 cactgttttg aggttgtgga tagaactgac gaagtcagct caaaacactg ttttgaggtt
    37561 gcagatagaa ctgacgaagt cagtaacatc tatacggcaa ggcgacgttg acgcggtttg
    37621 aagagatttt cgaagagtat aagaaaaaat cagtccccta aaggagcaga ttatgaagtt
    37681 attgtctatc gccatttcta gctataatgc agcagcctat cttcattact gtgtggagtc
    37741 gctaatgatt ggtggtgagc aagttgagat tttgattatc aatgacgggt ctcaggatca
    37801 gactcaggaa atcgctgagt gtttagctag caagtatcct aacatcgtta gagccatcta
    37861 tcaggaaaat aaaggccatg gcggtgtggt caattgcggt ttggcagagg cttctgggcg
    37921 ctattttaaa gtagttgaca gtgatgactg ggtggatcct cgtgcctact tgaaaattct
    37981 tgaaaccttg caggaacttg agagcaaggg tcaagaggtg gatgcctttg tgaccaattt
    38041 tgtctatgaa aaggaaggtc agtctcgtaa gaagagtatg agttacgagt cagtcttgcc
    38101 tgttcggcag atttttgact aggaccaggt cggaaatttc tccaaaggcc agtatatcat
    38161 gatacactcg ctgatttatc ggacagattt gttgcgtgct agccagttct aactgcctga
    38221 acatactttt tatgtcgatg atctctttgt ctttacgccc cttcagcagg tcaagaccat
    38281 gtactatctg cctgtcgatt tctatcgtta tttgattggg cgtgaggacc agtctgtcaa
    38341 tgagcaagtg atgattaagc gcattgacca gcaactcaag gtcaatcgac tcttggtaga
    38401 ccagcttgat ttgtcccaag tgagtcatcc taaaatgcga gaatatctgc tgaatcatat
    38461 tgaaatcacg acggtgattt ccagtaccct gctcaaccga tctgaaacag cggagcatct
    38521 ggcaaaaaaa cgccaattgt ggacctatat tcagcagaaa aatccagaag tctttcaggc
    38581 tattcgtaag accatgttga gccgtttgac caaacattct gtcttgccag atcgcaaact
    38641 gtccaatgtc gtctatcaaa tcaccaaatc tgtttatgga tttaattaat ataagtgttt
    38701 tataagaggg atttaagaaa aattttaact ttttcttagt cctttttaat ttcaggagat
    38761 tatactagag tcatcaaata aagaaagact ctaaggagaa tcctatgaaa ttcaatccaa
    38821 atcaaagata tactcgttgg tctattcgcc gtctcagtgt cggtgttgcc tcagttgttg
    38881 tggctagtgg cttctttgtc ctagttggtc agccaagttc tgtacgtgcc gatgtggtca
    38941 atccgacccc aggtcaagtc ttacctgaag agacatcggg aacgaaagag ggtgacttat
    39001 cagaaaaacc aggagacacc gttctcactc aagcgaaacc tgagggcgtt actggaaata
    39061 cgaattcact tccgacacct acagaaagaa ctgaagtgag cgaggaaaca aactcttcta
    39121 gtctggatac actttttgaa aaagatgaag aagctcaaaa aaatccagag ctaacagatg
    39181 tcttaaaaga aactgtagat acagctgatg tggatgggac acaagcaagt ccagcagaaa
    39241 ctactcctga acaagtaaaa ggtggagtga aagaaaatac aaaagacagc atcgatgttc
    39301 ctgctgctta tcttgaaaaa gctgaaggga aaggtccttt cactgccggt gtaaaccaag
    39361 taattcctta tgaactattc gctggtgatg gtatgttaac tcgtctatta ctaaaagctt
    39421 cggataatgc tccttggtct gacaatggta ctgctaaaaa tcctgcttta cctcctcttg
    39481 aaggattaac aaaagggaaa tacttctatg aagtagactt aaatggcaat actgttggta
    39541 aacaaggtca agctttaatt gatcaacttc gcgc
//
LOCUS       DAVLVZ010000016        38984 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000016 DAVLVZ010000000
VERSION     DAVLVZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.016"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            216..392
                     /locus_tag="VYN77_000748"
                     /pseudo
     CDS             216..392
                     /locus_tag="VYN77_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            575..781
                     /locus_tag="VYN77_000749"
     CDS             575..781
                     /locus_tag="VYN77_000749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1558233.1"
                     /translation="MREYRTYEEIAADFGIHESNFIRRSQWVEVTLVQSGFTVSRTPL
                     SSEDTVMIDATEVKINRPKKTISE"
     gene            807..1196
                     /locus_tag="VYN77_000750"
     CDS             807..1196
                     /locus_tag="VYN77_000750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1558234.1"
                     /translation="MKAQAIVTSQGRIVSLDITVNYCHDMKLFKMSCRNIGQAGKILA
                     DSGYQGLMKIYPQAQTPRKSSKLKPLTVEDKACNHALSKERSKVENIFAKVKTFKIIS
                     TTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(1248..1610)
                     /locus_tag="VYN77_000751"
     CDS             complement(1248..1610)
                     /locus_tag="VYN77_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558235.1"
                     /translation="MPKTKTLAKLADVILWSFDFANDHAHAFFMDNVEWSHADSYFFS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1964..3844)
                     /locus_tag="VYN77_000752"
     CDS             complement(1964..3844)
                     /locus_tag="VYN77_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1558236.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKINTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RHAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3838..4707)
                     /locus_tag="VYN77_000753"
     CDS             complement(3838..4707)
                     /locus_tag="VYN77_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1558237.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAGKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNCNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4790..7435)
                     /locus_tag="VYN77_000754"
     CDS             complement(4790..7435)
                     /locus_tag="VYN77_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1558238.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDMATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7526..8806)
                     /locus_tag="VYN77_000755"
     CDS             complement(7526..8806)
                     /locus_tag="VYN77_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1558239.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFVELDLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8977..11061
                     /locus_tag="VYN77_000756"
     CDS             8977..11061
                     /locus_tag="VYN77_000756"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1558240.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNPLMLFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDVAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11101..12780)
                     /locus_tag="VYN77_000757"
     CDS             complement(11101..12780)
                     /locus_tag="VYN77_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1558241.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDAALPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQVLPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            13535..14764
                     /gene="arcA"
                     /locus_tag="VYN77_000758"
     CDS             13535..14764
                     /gene="arcA"
                     /locus_tag="VYN77_000758"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1558242.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELILSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            14822..15838
                     /gene="argF"
                     /locus_tag="VYN77_000759"
     CDS             14822..15838
                     /gene="argF"
                     /locus_tag="VYN77_000759"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1558243.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            16003..16950
                     /gene="arcC"
                     /locus_tag="VYN77_000760"
     CDS             16003..16950
                     /gene="arcC"
                     /locus_tag="VYN77_000760"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1558244.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVIVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            17159..18670
                     /locus_tag="VYN77_000761"
     CDS             17159..18670
                     /locus_tag="VYN77_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1558245.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            18692..20023
                     /locus_tag="VYN77_000762"
     CDS             18692..20023
                     /locus_tag="VYN77_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1558246.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVKLLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(20429..20719)
                     /locus_tag="VYN77_000763"
                     /pseudo
     CDS             complement(20429..20719)
                     /locus_tag="VYN77_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20707..20958)
                     /locus_tag="VYN77_000764"
     CDS             complement(20707..20958)
                     /locus_tag="VYN77_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1558247.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFDGMGDQPVQDTE
                     TSSALILSHYLDEQDLSEKLKSELQWLELENKLLNLWEH"
     gene            21162..21239
                     /locus_tag="VYN77_000765"
                     /pseudo
     CDS             21162..21239
                     /locus_tag="VYN77_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(21537..22421)
                     /locus_tag="VYN77_000766"
     CDS             complement(21537..22421)
                     /locus_tag="VYN77_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1558248.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(22566..23717)
                     /locus_tag="VYN77_000767"
     CDS             complement(22566..23717)
                     /locus_tag="VYN77_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1558249.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(23863..25629)
                     /locus_tag="VYN77_000768"
     CDS             complement(23863..25629)
                     /locus_tag="VYN77_000768"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1558250.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAATSHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTRHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGVSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(25642..26370)
                     /locus_tag="VYN77_000769"
     CDS             complement(25642..26370)
                     /locus_tag="VYN77_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV1558251.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIRCYEEREDVRAVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            complement(26385..29402)
                     /locus_tag="VYN77_000770"
     CDS             complement(26385..29402)
                     /locus_tag="VYN77_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEV1558252.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETA
                     RVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVISYDISGQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYSSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGVYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSGSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKVNENVKQTGKLKFNSLEMDVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSN
                     AQDANQAKKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTN
                     NSYDQPSLNFDQKNHMVTITINSNGNLEFELHF"
     gene            complement(29413..31629)
                     /locus_tag="VYN77_000771"
     CDS             complement(29413..31629)
                     /locus_tag="VYN77_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1558253.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLDPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLSTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLFQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSRFDEFFYGDELMNAGIKVSLSNLALCVPEYLTKLFVIEEVVCKY"
     gene            complement(31651..33522)
                     /locus_tag="VYN77_000772"
     CDS             complement(31651..33522)
                     /locus_tag="VYN77_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HEV1558254.1"
                     /translation="MKIENKNVRRNFFWGDGRFYTTDIVNKRAGVMIKNVSKEEFTIT
                     LENGIRLSSNHFSAIVREEGDTRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTEV
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYLGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRDGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKHLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKRILEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTVVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDIQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMDHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANARAVKWIEENYQTLKYSKKIGGSPEKFEIYGYKCH
                     NQKTSTEILSLRNPAQIKQKIKIENLSIENFTRVIGDFTIQEDEIELAPYSIVILKK"
     gene            complement(33528..34847)
                     /locus_tag="VYN77_000773"
     CDS             complement(33528..34847)
                     /locus_tag="VYN77_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1558255.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNGNTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            complement(34844..35683)
                     /locus_tag="VYN77_000774"
     CDS             complement(34844..35683)
                     /locus_tag="VYN77_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1558256.1"
                     /translation="MNTHINGISKKGKVLIYGYMLLTILISIFPIAWIFLSSLKADPM
                     KNPGISLPTDFTLEGYINVFTKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARME
                     FRGKKLVTSMLYSTLFIPATAMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFII
                     KNYFEIIPKELEEAAEIDGATYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASM
                     LVIDKNELTVPALLGQFTTSFNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGA
                     VKG"
     gene            complement(35686..36615)
                     /locus_tag="VYN77_000775"
     CDS             complement(35686..36615)
                     /locus_tag="VYN77_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1558257.1"
                     /translation="MAQKIMSLQNRKNQKRRFIFLFLLPTLICFFLFYFYSVVTIFLT
                     SFAKWDYTNLNTPEFLGFDKLFENYRYVFKEYPFFTEALINSVRWAVIGVIIQVPLAV
                     SVAITLSKKLKGWKISRNLYIVPSIISSAAMGLIFLQIYNPNYGVVNQIIHLFNPSFK
                     DSVLLTPGLNIVAMTGAYIFFAGASTIMILGQIFAIPEEVQEAAILDNITGWRKEWYI
                     TIPMIKGTIKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFVIRELAVASSRTQYAR
                     ANTIGVIQILGGMLIIVCINILFRERKRLKGGK"
     gene            complement(36703..37995)
                     /locus_tag="VYN77_000776"
     CDS             complement(36703..37995)
                     /locus_tag="VYN77_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1558258.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLAAIEQKLVLDLKPYIDSNKLEKSVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPDQWNTWDDFTQAMASIRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYPAGVAISSSGGGITISSKMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKAVVPTVSDVW
                     GGDVHTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
     gene            complement(38024..>38984)
                     /locus_tag="VYN77_000777"
     CDS             complement(38024..>38984)
                     /locus_tag="VYN77_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1558259.1"
                     /translation="TNKILLMPDLFNYLLTGKFATEKSIASTTQLFDPRSQNWNQNIL
                     KLFELDSSLLPEIVSEGNVLGRIKKEYGLGDIPVVNVCSHDTASAIVSVPKTEGSLFI
                     SSGTWSLVGVELTSPILTTESFSYGFTNEVGKDGVITFLKNCTGLWIIEELRRSFERR
                     GKAYSFDDIRTMVEKEKENLPLIDTESTEFATESDMHKTLTEYLAYHHETREWTDGQL
                     FKIVYESLAETYRKAIELLEELTHKVYKRIYVIGGGARASYFNQMIADRTGKEVLTGS
                     TEGTAVGNIVVQLLSQGKINEDTELKDIMTNIADTQYYYPQLS"
BASE COUNT        11622 a         8414 c         6897 g        12051 t
ORIGIN      
        1 tgtcatctct ccataaacac agtatatcat ttttaaattt taagtcaagg aaaattaacc
       61 cactttaatt tcttttttaa cccgtgttaa aaaagaaatt aaagtgggtt aaaaaagaaa
      121 ttaactgagt aaaaactctc gaaattcaat tcgctatatt atcactcaac ttgaacaaat
      181 accagaacca caaaaaacag atgtaaagga aaaatatgga cttaaaattt gaaggggttg
      241 atttagaata caaaaaagct aaaaacaatc ttccagagtc tttctgggaa acctactcag
      301 catttgcaaa tacaaatggc gggaaaataa ttctcgggat agatgaaaaa aacattgaca
      361 cctaccaaag agtcaataga ctccctgcga aacaaaatta tgaagcaagt aaacaactaa
      421 ccgatgcacg atttaagcgt cttgttggtg ttcaacgcac gacttttgaa gagatgttag
      481 ctgtattaaa aacagcttat caacttaaac acgcaaaagg tggacgaaaa cctaaattaa
      541 gcctagaaga ccttcttatg cccactcttc aatagtgcga gaatatcgaa cttatgaaga
      601 aattgcggct gattttggta ttcacgaaag caactttatc cgtcggagcc aatgggttga
      661 agtaactctt gttcaaagtg gttttacggt ttcaagaact cctctcagtt ctgaggacac
      721 ggtaatgatt gatgcgacgg aagtaaaaat caatcgccct aaaaaaacaa ttagcgaatg
      781 attctggtaa aaagaaattt cacgctatga aggctcaagc gattgtcaca agtcaaggga
      841 gaattgtttc tttggatatc actgtgaact attgtcatga tatgaagttg ttcaaaatga
      901 gttgcagaaa tatcggacaa gctggtaaaa tcttggctga cagtggttat caagggctca
      961 tgaagatata tcctcaagca caaactccac gtaaatccag caaactcaag ccgctaacag
     1021 ttgaagataa agcctgtaac catgcgctat ccaaggagag aagcaaggtt gagaacatct
     1081 ttgccaaagt aaaaacgttt aaaataattt caacaaccta tcgaaatcat cgtaaacgct
     1141 tcggattacg aatgaatttg agtgctggta ttatcaatca tgaactagga ttctagtttt
     1201 gcaggaagtc tattgaaata ctaagcctac acagactgat ttcaatttta ccattcttca
     1261 tagtcaaaac catcataatc tggatattgt tctggcgacg ttccaaccga acgtacgaga
     1321 taagcttctt cgtcctctgt ctcgatttct ctcagcactt ggcattcaaa acgccattca
     1381 tcaccgaagt cgaaaataaa cttaaatttt tgtttgacac ttaggctatc cagatagaca
     1441 ttttctgtgt aacgttcttc aacatcgtca ctaacaaagc taaaaaagta agaatctgca
     1501 tgactccact caacattatc catgaaaaat gcgtgagcat gatcatttgc aaaatcaaaa
     1561 ctccataaaa tcacatctgc tagtttcgct aacgtttttg tttttggaat ctatatatgt
     1621 cggtagcaac cttttttaaa tttgacggaa atgacccaag cttctatttc ttgttttact
     1681 aattttaagt gtagcataat acctccaatt gctcatcgat agaattgctc ttaggaaaca
     1741 ttcactttct gctattttat cataagtttg tctaactgta gcatttgaaa ccagataatt
     1801 cttctgtttc acgcgaaaca ccctgcgcca agcaaggtgt tcttgttttt atactcaatg
     1861 aaaatcaaag agcaaactag gaagctagct gcaggctgct caaaacactg ttttgaggtt
     1921 gtagatggaa gctgacgtgg tttgaagaga ttttcgaaga gtattaagtc gtataaatcg
     1981 tcgacgctgt cgcaatggta tgccactggt tagctgtagt tgctgcgaag tccttgtctg
     2041 cgtagaagtc agtaaatggt aggatttcaa cttccagctc gtcgatgcgg tcaagctgac
     2101 cagccagata aacctcgata cggctttctg ctcgtttgat gcgttgcaag agtccgccca
     2161 tacggatgtc aactgtatcc aaaccaaaga ccttgttttc tttcagccat tggtggctaa
     2221 agagggcatg gaagtcttca atttggcttc taagttctgg taattcttgt ctggcgattt
     2281 gttgtaaact ttctttatca tccgcttggt aggcatgacg aatgcgtcgt cccacatcta
     2341 ctttgctact taaaatagca ttcaactggg cctgagtttc aaagagataa gcatagtttc
     2401 cagctttttc tttaatgtta gcaagcgtct cagcagcctg agcgaagtgc ggtttgtcct
     2461 gttcaggtgt catgtgttga tcaagaatcg gacaaagaat atcctgataa aaaacatagc
     2521 ggttgggatt gataccgctg agattgcctg gtaggtctgg taagaggttg gcaaggtcaa
     2581 tctgcataaa atcctcaacc gttagaccag tattgatctt gaagtgcgca gacaaaccat
     2641 ctaggtcatt gcgatagctg agttctgccc agatttgcaa gcttggtagg atagagaact
     2701 gggcagtttc accaccattg tctccccaac ccgttacgat gacttcttta atctgattgg
     2761 cacggcaggc tttattagcc tcgatagcca ctagacggct aaaatggttg tgaggtgtaa
     2821 agccaatcca cttccaagct ccccctgcaa atgcaaggtc atggctaatc ttgtgatgat
     2881 tgcggaaatt acggttgtat ttttcctcgc tatcctgata ataatcccag taaaccagag
     2941 tcacacggtc tttgagacgg tctaggtaga cacgagtttc ctctggaatt tccacatcac
     3001 gatcgtactg gccatccgct gacatgagtt tgaagaacat atcactccac atctggcagt
     3061 ggaaaccata tttgtcagca atatccagca cgcgctccaa gtgttggcac atgaggagac
     3121 tacgatccac aacaccgttc aaaatcaggt agcgtcccaa accaaccaag tgggcttcgt
     3181 ccatcccgat attgaccttg cgagtcttca gtttagacaa cgtggcaaac atgccatcaa
     3241 tcaggtcata aaccttttct tcgccaatga gaagaatgtc ctctacatca cggagctcct
     3301 gcacttcctt gacaccccat ttgacaaagg ccgacaagtg ggccaaggtc tggatgcatg
     3361 gtacaaaggt catgtcaaac tgttgagcat aggcttcgat ttcctgcaac tcctctgctg
     3421 aataagctcc acggaaatag ccaaagtaag gctgcccttc aatctggtaa gtgtcttcca
     3481 tgtaaagctc aaaggttgag tagcccatga gagccaagac ctcaatcatc tgcttggcag
     3541 aagccacatt cagcaccgca tttcgagaac agtcaaccat gtaagccaaa tcttcgtaag
     3601 ctgcttgttc ctcaatctct actttatcag cttctgctag aactgttacc aacaaggata
     3661 aggcacgata aagttggtga ggtttgcggt aggtcaattg atagtgacct tcctcaccct
     3721 ggatagagat agaggcttgg tcagactgag tgacagccac ttccacatct ggtagagaaa
     3781 tgtgaccttt taaaacctca atagcttgcg tttgtttggg actaagtcct gtaaatctta
     3841 ccattgcttt tcctcctgta gccagttgac aagggcaccg tagagatttg catctgcgtg
     3901 ataggtgcag gcctggataa ctggggcgac cgtgtattct tcgtaggtat cgacaaagtt
     3961 atcaacagcc ttcttgacac cttggataaa atctggattt tgactgatag atccacccag
     4021 actgatgaca tctggatcga tcagatactg gatattgagc aagccttgcg ccagattgca
     4081 gttcatgcgc tcaatggctt cttgacaaag agcattacca gctgcggcct cttggtaaat
     4141 cttgcgaccg tcccaatcag tatgaccaga tttttcaatt acgtatcgta ccatattccc
     4201 agttgatgct agttgcgacc agttattaag ttttccagca ggggcaaggg ttgtcatgta
     4261 gccaaattct ccacccagac cgtggcgacc tcgatgaagt ctaccattga taatcatggc
     4321 tccgccaatc cctgtcccaa tcacgacaca ggctgcattt tcaagctctg gatgagctag
     4381 tagttcactg agtccaacgc agttggcatc attttctaaa tggacaggta gctgataaga
     4441 gctaagagcc tcataccaag aaaagccatg gatgtagggc accgcactga agccatcaat
     4501 cacacctgtc tcttgattga ctgcacctgg aacgctcata gcaatcccac tgtaatcctg
     4561 ttctgacaag cgttgatcta gccacgctag taaatcctcc aagttttcag gcgttgaaat
     4621 acttgtctta tccagtattt tcccatcagg agtcagactg gcaaacttaa tcccagtccc
     4681 tccgatatca atcgttgcaa tggtcattgt ttttaccata aaaaaggcga atcagcagtt
     4741 agttcttact ttttcgcccc tcctttcttt tgatgtttac tttttgaaat taaatttctt
     4801 cttttttgat gaattctgta cgaatctctt gtggagccaa tagtcctgaa tgaactggat
     4861 atggtcgctc aagtaagtca agaatggttt gttgatgttc tgatatgcgc acattttctt
     4921 gactcatatt atagtaacgg aggacatagc cttcttcatt ttccgccact ttaaaggctg
     4981 ttggacaaac ttgcggtatg ctgagaacag aatggctcaa gaggctacca gtcgcaacca
     5041 cgcttccttc ctgtctagca agctgaaggc tggtaaacgg tgtctgcaag gctttggcac
     5101 gacgataggc tgagaagcgt tcttgggctt ggtggcattc aagtgcaaac tcgacttcaa
     5161 actcacgcaa gcattgtgct tctggcgttg ggaagtagcc ccagtcacct agctcacctg
     5221 atgcacgcaa aatggtcacg gcaatggtgt tatccccaag gatttcgtat tcattcaatc
     5281 ccttgttgga tacagtcacc cctttttcat cgtcatacag actgacaaaa gcttgttggt
     5341 gttgaggatt ttcaggattt tcccatgaag cagctggttt gtttggtcgt gtcaccacct
     5401 catagatact ttcagaatca ttgcttggac gcgtgttatg agtcttgacc aagagacgga
     5461 tacggtgatc cttggcagtg ttagtaaagc gagtcttgaa gcggatttgt ggattgtcaa
     5521 cgaagacagt caactcagtt tccagaggaa tgtttgtcaa ttcttctgac cgtccagcct
     5581 cacgcttcat aaactcgatg ataccttgtt gctcttcttc tagcttttca tccgcactga
     5641 caggcacggt caattcatgt ttgagcaaga ttttagcata gcgatctgtg ttttccaata
     5701 cctcgtggcc cttaagctct gcaaagattg gctctgttcc ttttggttgg aaatagatat
     5761 actcgtttcc gatgtcaccc cggtcttcaa agcggataaa gtcttcatag gcttcgtgag
     5821 ttgtcttgtc atagactgtg atgttgtcat ccacactcac cgttacgaat ggtgtatcaa
     5881 tcactccgtt ttggtaaata ccctcacggt gttcttgttt tccttccagc aattggaagg
     5941 ttgtccaaga aagcggtgct aggtgaactg gaatggtcac acgcacttgt cgagcaatac
     6001 gagcttggcg gaacttatct tttggtaaat cgtattcaaa attagctccg aggtcttcga
     6061 ttgtagcctc tacaggacga ccatccaagt cctccacacg gtaacttggc aagataagag
     6121 cagccatctt tttgtagcct tctgttggat gcaattcctt gaaatcacaa gtcgccatat
     6181 caatcactgt gctgacagta tcgaccttat catgcaagcc tgtgttaatg acagtaaaga
     6241 gatagtcact ttgagcctta tccgtagcaa ttttaccctt ccactcgttg agcaaattac
     6301 ttttaacaaa gtttcctact tggttgacct tggcaaaacg cgtttccatc tcgcggtgaa
     6361 cttcgtccac gctacagcca cagatactat catgtggcgc attctgcaaa agtgttttcc
     6421 aagcataggt caactggtcc ttgtggttgt gtccaccagt gataatagtc aagggttcta
     6481 ccacttgctc taggaggttg ctattttctt ggaaggcttg ttttaggtaa atgcgggatg
     6541 aagaagtgtt ggcaagtgtg taccagccat ctgtttcctg actggtcaac tcgcctgtaa
     6601 cagttgataa gtgttcagga agcgcacctt ctacagcttg aacatattca tcaaaagaac
     6661 tatgaacaaa gattacatcc gggaagagtt catttgccac acgaatggct tcgctcagat
     6721 ttttctgtac aggctggtgg tcacagccgt tcatcatcaa ccattggttg gtcgaagcgt
     6781 aggcacgcac atctgacaat ttttgtttcc agaaggtcaa ggcctcatct ttgtcaactg
     6841 ggatttcatt cccattactg taccagttgg caaagagaat acctaaaaca cgactaccat
     6901 ccacaccctg ccagtacatt tctgaaaact gggatgtaaa ctgctcatct tcaaggactt
     6961 ggttgtcaaa tccaatcggc ttcacaccac gaccaaaggc cgccacgtga atgcctgatt
     7021 tttgaagaat ttgaggcgct tgtcccatat ttccaaaggt atctggaaag tagccaatct
     7081 gggttgattt accccatttg gcagcttctt gttgaccaat caaggtattg cggacattgg
     7141 cttcactgga gatcaagtag tcatcctgca agatgtaaaa gggaccaatt ttaagtttgc
     7201 cctcgtcaat gtagcgttgg accttgtcgc gattttcagg gcgaatttgt aagtagtcat
     7261 caaggacaat agtttgtcca tccaagtgga aactcttgaa ctcagggtca ttttcaaaga
     7321 gatcaaagag attgtcaaac aattccacca actgcatacg atggctttca aaaggcaagt
     7381 accactcacg gtcccagtga ctatgtgaga taatatgtac aacaacattt tccatgaagt
     7441 aaaacctcat tctaaattta aattttaacg ttttaaatgt aaacttgtga ttctgataag
     7501 aatcggttga gctaaagcga gccccttagc gaatatccaa gtaatccaag accaactcac
     7561 agaacatcat gttagcccag gagaaccatt cacgagagta gagggtcgga tcatctacgt
     7621 ggaagctttc gtgcatgaca cctgtaccac catcgcaggc aaccagctga tccagcaaga
     7681 attttttctc tgccttatct cttgttgtca atccttggat agaaagggcg attggccaga
     7741 tatagcgata gaaggtatga gaacttccga gaccgctagc gtattctcct tggtagaagt
     7801 atggattttc agagctcaga atggtacggc gagtggcttg atacacttca tcatcgatcg
     7861 aacagtagcc cagatagggc gcagctagta gacttggtac atttggatca tccatgatgc
     7921 tggcatttcc taggccatct acttcaaaag cgtaaatctt ttcgcccttg ctgttggtgg
     7981 tgtaagcgta gttttcgatt ccttcttgga tttcatcctg aagacgcttg gcatcagcaa
     8041 taacactctg gctatcagct aggtctaatt cgacaaagat ttcttgcaca taacccaaga
     8101 ctactacggc aaacatatta gacggaatca agtaactata ctgacagcaa tcgtcactcg
     8161 gacgaaaagc tgaccaagtc atacctgtca ctgcaaagtc aggtccaaag ccatcattta
     8221 ccaaggtgtc ttccttacgg tccgtatctc ggacaaaacg ataaggagag ttcttgtggt
     8281 cttgttcaac tgtccataga tggagaattt ccttagtcgc tgcgacaaaa gtttcatcaa
     8341 actgactagt ctcgccagtc tctttccaga ggagataagc caactgcaaa ggatagcaaa
     8401 gcgaatccac ctcatacttg cgctcccaga tccagccgtt aaggtctgtg tggtcagtct
     8461 cgtggtgccc tttccagttc tcctcaatgt tgaaggagtt agcataggga tccttgagta
     8521 ccaaggtcat ctgacgtttg accaaacctg caatggtctg acgcaggagg gcatctcttt
     8581 tagctacatg aaggtagggt ctgagttggg ctgtcgaatc tcgaagccac atggcaggaa
     8641 tatccccagt caagacaaaa gttgaaccat cttctaagat ttcaaccgta ttgtccaagg
     8701 tatcggtgta gcaacgctcg aaaacatcca cccactctgg atggtcctta gcccgctctg
     8761 ctacttcatc tagccattct ctaacaattt ctttcgaata aatcatcgtg caatttcctc
     8821 tttcaaacaa gtttcatttt cagtatatag cttttgagac agaaaataca tacacaaatg
     8881 atagtcattt ttctacaaaa tagttatttt tgaaactcct tttctaaagg ccctcttttt
     8941 ctgatataat gtagaaaaac agctaaagga aagactatga aaccactact tgaaaccatc
     9001 gatacccgct ttggtactgc tagcaagcat gccttctctc ggggaaatac cctgccatac
     9061 acaggcgtgc cttttgggat gaattatttt gtgccccaga ccagtgacca ggatggatct
     9121 tggttttttg atccgcatct gcctatcttt caggggattc gattaactca ccagcccagt
     9181 ccttggattg gggactactc ttggctcctt ctgacacctg tcaccagtca actgggggga
     9241 gacagcctct ttcaccgcca gtcttcttat gatatagata aagcttgctt ccagcctcac
     9301 tatttgaagc tcttttccct gcgctatcaa attgaaaccc agctcacacc gacttgctat
     9361 ggtgcttcta ttcgtttgaa gcaaaagcaa ggcaaagccc tctccctcta tcttcacgca
     9421 gcagatgaac taacagttga gcaggtagat aagcggactc tggctctcag acaagaaggg
     9481 aaaacagaga ccaataaaaa tccactgatg ctcttcactg ccctgcaaat gaacacggat
     9541 attcttgcta tcagccaaga agctggagac tggcgaattg acttagcaag tagtcaaacc
     9601 gagatgcaac tagccacttc tttcatctct ccttctcaag ctctaatcaa tctacctcaa
     9661 gaagattttg atagctgtaa aacaagtgcc caagcggatt gggaaaatct cctccatcgt
     9721 tttgacatta tagagacagg agaggctgac cgaaccttct ttgaccactg cctctacaga
     9781 ctcttcctct tcccacagac tttttatgag gttgatgagt cagggcaagc catccacatg
     9841 gatctggcta ctggtactgt caagcctggt gtcctcttta gcaacaatgg tttctgggat
     9901 accttccgca ccaccttccc cctctttgcc cttatcatac cggagcacta tcaacgcttt
     9961 ttagaaggtt tcctcaatag ctaccgcgat actggtttcc ttccaaaatg gctggctcca
    10021 gatgaacggg gtatgatgcc aggtacactt ttagacggta ttatcgcaga tagcgcctgc
    10081 aaggacgtgg cccctgacct agaaggagaa ctcttccaag ccatgctcga aacagccagc
    10141 aaagccgacc ctctcggaat caatggccgc cacggactag cccaatacca agaactaggt
    10201 tacctctcta ccgaccacca cgaaagtgtt agccatactc tagactatgc ctatagtgac
    10261 ttttgtatcg ctagctgtgc caaaaaacta gagaacatag aaatcgctga aacctacaag
    10321 gctgcttcac aaaattaccg ccagctattt gacgctgaga caggttacat gcgagcacga
    10381 gacaatcaag gaaactttca ccctgacttc tctccttata gttgggggcg agactacgct
    10441 gaatgctctg ccattcaagc tactttaggc gttctccacg acatccctgg cttaatccaa
    10501 ctgatgggtg gaaaagaaac ctttagcaac tatcttttga aagcttgtca agatgctccc
    10561 ctctttgaaa caacaggcta tggttacgaa attcacgaaa tgagcgagat ggctactgct
    10621 ccttttggac aaatagccat ttccaaccag cctagtttcc acattcctta tctcttccgc
    10681 tacagcgatt accctgacta cactgccctt cttatcaaga cgctccgtca gaaagctttt
    10741 cacccaagtt gggaagccta tcctggagat gaagacaatg gtagtctctc tgcttggtac
    10801 atctggtcag ctctcggatt ttatccaacc tgtccaggaa aaccaagcta tgacctcgga
    10861 atccctctct ttgaccatct acgagtctac ctcgctaaag aagataaatg gctggatatc
    10921 catactaaac aaaaccacaa ccattttaac tttgtcaaag aatgccgact ggacaaaaca
    10981 ctcgtatcaa ctattcaaca ccaagacctc ttaaaagctg aacaactaac tttcaccctc
    11041 agctggttac caagtcacta ataaccaaaa gaacctttgc atcgcgcaaa ggttcttctt
    11101 ttatgaaact tggacttgaa gttgatccac aagctgacca tccaccgtta ggttcagata
    11161 gaaatccaag gttttatctc ctgcgaaata cagggttggt agatgcaaat cttttttgct
    11221 ttcccctgct tggaattgaa ggacttgaat ctcatcctga taggcgacac catggacacc
    11281 tgtccccggt tgaatcgaaa tcttagcctc taaagaggag ctactttcac tacgttcaat
    11341 cctaaaatga agagtctctc cctttgccac agctaggctt ttttctgcaa atgctagccc
    11401 ctgaacagct actgtttgtg ataacctcgg agttttatag agtgaaatct tggtcaacaa
    11461 aggcaaaacc tgtgattctg taatgactac acgtatcttc tgtgcttcaa caactgctcc
    11521 tcgtaagaga cgtttgtaac caacagtatg acccgaacca aactcctgcc agacaccatc
    11581 cacctctact tgcacatgaa aagcagcgat tcgttgccct agcttcaaat cttctcttaa
    11641 ctcaattaca tcaaaagttt taggagaacc taagtcgagt tctaactgga tgggcaaggc
    11701 tgcatcgttt gcccaagagc tggtctcaag gccgtctgtc aaatggcgac aagcaaagtc
    11761 tgcggaaaga gctggaccag atacctcagc tcccagagcc aaatcttctt tatagagctc
    11821 attgcgatag gtcgcaaatt cataaagtcg ttcaatatcc tttgcatcaa agagcccagc
    11881 ttgattcggc ggaatattaa gcaagagtgg agttcctcgc cctactgagt gaaagtagat
    11941 ttcgaccaac tcctcgagag acttaggatc ctgatcctca tggtagaacc agcctggacg
    12001 gatggaaaca tctgcctctc cgattgaaaa aatcgtgccc gagggatccc cgtgctgaag
    12061 atagttcagc tctgcttctg ttcctagttt atcaggattc accttttgcc acagtggatc
    12121 acctgcatac cctcgttcat tgccaatcca gcggatactg gtgccttctg ttgaaaaaat
    12181 caagcaagcg ccctgcaggt cacgaatggt ttcaaaccat ttttcaaatt cataattaac
    12241 cttttgcgcg ccctctcctc tggcaccatc catccaaacc tcagcgaact taccagcatt
    12301 cccatagtta ggatttgata agatttcctt caactgagcc agataatagg cattgtagtc
    12361 cgcttctcgg tccacatgat agaggggact atgggcatcc cacggtgaca ggtagacccc
    12421 catatccata tcaaactctg tggcagcttg ggatacttca aggagcaagt cgccctttcc
    12481 tctcctccaa ggactgacct taaccgaata atctgtgtga gctgtcggat aaaggacaaa
    12541 gccatcgtgg tgcttgacca ccaaaatcaa ctttttgaag cccgtttcct tgagcacacg
    12601 aacccactca cgcgcatcca actgactcgg gttaaagcgc tcaggatcct cctgtccagt
    12661 cccccattct tggtcataaa aggtattagg accgaagtgg ataaaagctg ctagttcatc
    12721 tcccagataa gctagctgag tctgacttgg taacggtcca tgcggtttaa ttttcttcat
    12781 cgttatatat ctccttcttt agcaacttct cttgttcaca aggttatcca caggttgtgg
    12841 ataataaacc atgatctaac cgccagctct ttccaactcc aactggtagg ctcaggattt
    12901 ttctcctcaa tattccctct tcaaacagct ctgtcatatg gttctgctaa cacctctcag
    12961 aatgctactc tccataatta ttctcaggct tctttcaggt cattgtgtga agaccattca
    13021 ctctccagac ttttccacag attgtgaata agtgattatt ctaaactatc acctcgtcga
    13081 ctataacaaa aaataaaaag agggtcaata agataattat ctcttcctaa atactcagaa
    13141 aagattctat aatttgtaca attgaacaag aaatgcttct ccaaaatcta tcttcaaaag
    13201 aatgttgcat ctttttatcc tttcacacaa acatttctct ttatacagat ccaagtgata
    13261 accagatgat aattggacac tttttggtca tttgtatatt ttttgaaaaa ttttttaaaa
    13321 aaattttata tcctttttat agaagtttat gcatttgtat ctatgtattc tatcctatgt
    13381 tatgtataat aacataaaat tgtgctaaac atcataattt atattatttt ccgagaaatt
    13441 atagccattg acattaccta ggaattgcgt tataacatac atgtaagcgg tacccaaaat
    13501 ggtgccaagt caaaattttt aaggaggaaa atacatgtct tctcatccaa ttcaggtctt
    13561 ctcagaaatt gggaaactga aaaaagttat gttgcaccgt ccaggcaagg agttagaaaa
    13621 cttgttgccg gactatcttg aaaggcttct ttttgatgat attcctttct tggaagatgc
    13681 tcaaaaagaa catgatgcat ttgcccaagc tcttcgcgat gaaggaattg aggttctcta
    13741 cctagaacaa ctcgctgctg aatcattgac ctctccagaa atccgcgatc aatttatcga
    13801 ggaatactta gacgaagcca acatccgtga tcgtcaaacc aaggttgcta ttcgtgaatt
    13861 gcttcacggc atcaaggaca accaagaatt ggttgaaaaa acaatggctg ggattcaaaa
    13921 agttgaattg ccagaaattc ctgacgaagc taaagatcta actgacttag ttgaatcaga
    13981 gtatccattt gcaattgacc cgatgccaaa cctctatttc actcgcgacc catttgcaac
    14041 aattggaaac gccgtatcgc ttaaccacat gtttgcagat actcgtaacc gtgaaacact
    14101 ctacggtaag tatatcttca aataccaccc aatctatggc ggaaaagtgg atttggtcta
    14161 caaccgtgaa gaagatacgc gtatcgaagg tggagacgag ctaattcttt ctaaagacgt
    14221 ccttgcagta ggtatctctc aacgtacaga cgcagcttct atcgaaaaac ttttggtcaa
    14281 catcttcaag aaaaatgttg gcttcaagaa agttttggcc tttgaatttg ctaacaaccg
    14341 taaattcatg cacttggata ctgtcttcac tatggtagac tatgacaagt tcactattca
    14401 cccagaaatc gaaggcgacc ttcacgttta ctcagttact tacgaaaacg aaaaacttaa
    14461 aatcgttgaa gagaaaggtg acttagctga acttcttgct caaaaccttg gtgtagaaaa
    14521 agttcatttg attcgttgcg gtggtggcaa tatcgtagca gctgcgcgtg aacaatggaa
    14581 cgacggttct aacactttga ccatcgcacc tggtgtggta gttgtttatg accgcaatac
    14641 cgtgaccaat aagattttgg aagaatacgg gcttcgcttg attaagattc gcggaagtga
    14701 attggttcgg ggccgtggtg gacctcgttg tatgtctatg ccatttgaac gtgaagaagt
    14761 gtaatcgctg ttcggcattc gtcaatagaa aatgtaaaaa atagaaagag gaaataataa
    14821 aatgacaaat tcagtattcc aaggacgcag cttcttagca gaaaaagact ttacccgtgc
    14881 agagttagaa taccttattg gtctttcagc tcacttgaaa gatttgaaaa aacgcaatat
    14941 tcaacaccac taccttgctg gcaagaatat cgctctccta tttgaaaaaa catctactcg
    15001 tactcgtgca gcctttacaa ctgcggctat cgaccttggt gctcacccag aatacctcgg
    15061 agcaaatgat attcagttgg gtaaaaaaga atctactgaa gatactgcta aagtattggg
    15121 acgtatgttt gacgggattg aattccgcgg attcagccaa cgtatggttg aagaattggc
    15181 agaattctca ggcgttccag tatggaacgg tctaactgac gaatggcacc caactcaaat
    15241 gctcgctgac tacttgactg ttcaagaaaa cttcggtcgc ttggaaggct tgacattggt
    15301 atactgtggt gatggacgta acaacgttgc caacagcttg ctcgtaacag gtgctatcct
    15361 tggtgtcaat gttcacatct tctcaccaaa agaactcttc ccagaaaaag aaatcgttga
    15421 attggcagaa ggatttgcta aagaaagtgg cgcacatgtt ctcatcactg aagatgctga
    15481 tgaagcagtt aaagatgcag acgttcttta cacagacgtt tgggtatcaa tgggtgaaga
    15541 agacaaattc gcagaacgtg tagctcttct taaaccttac caagtcaata tggacttagt
    15601 taaaaaagca ggcaatgaaa acttgatctt cctacactgc ttgccagcat tccacgatac
    15661 tcacactgtt tatggtaaag acgttgctga aaaatttggt gtagaagaaa tggaagtaac
    15721 agacgaagtc ttccgcagca agtacgctcg ccacttcgat caagcagaaa accgtatgca
    15781 cactatcaaa gctgttatgg ctgcgacact tggtaacctt tatattccta aagtataatt
    15841 ttagataata aaccgtctac caacagctat gagggctgcg actaatagct ttagtccggt
    15901 cctcttttat ataatggtaa tctattattt cttataaaat atgtgaaaaa tcattaaatt
    15961 gaaatctaaa cgcattctat tgagtgtgat aaaggagaat ttatggcaaa tcgtaaaatt
    16021 gtagtagctt tgggaggaaa tgcgattctt tcttctgacc catcagcaaa ggctcaacaa
    16081 gaagctttag ttgaaacagc taagcatctt gtaaaattga ttaaaaatgg agatgatttg
    16141 attatcactc acggtaatgg acctcaagtt gggaatctct tgctccaaca tttggcatca
    16201 gactctgaaa agaaccctgc cttcccactc gactcacttg tcgctatgac agaaggtagc
    16261 atcggtttct ggttgaaaaa tgctttgcaa aatgctctct tggatgaagg catcgaaaaa
    16321 aatgttgcct ctgttgtaac gcaagttatc gtagataaaa atgatccagc ttttgttaac
    16381 ttgagtaaac caatcggtcc tttctattca gaagaagaag caaaagcaga agccgaaaag
    16441 agcggagcga ctttcaagga agatgctggc cgtggctggc gtaaggtcgt tgcctcacca
    16501 aaacctgttg acatcaaaga aattgaaacc atccgtactc ttttaaataa tggtcaagtc
    16561 gtcgtagctg caggtggtgg cggtattccc gtcgtcaaag aaaacaatgg acatttgact
    16621 ggtgtcgaag cggttattga taaagacttc gcttcccaac gtttggcaga attggttgat
    16681 gcagacctct tcatcgtttt gacaggtgta gattatgtat ttgttaacta caacaagcca
    16741 aaccaggaaa aattggaaca tgtgaatgtt gcccagctgg aagaatatat caaacaagat
    16801 cagtttgcac caggtagcat gcttccaaaa gtagaagcag ctatcgcttt tgtcaatggt
    16861 cgtccagaag gaaaagcagt tattacttcc cttgaaaatc taggcgcctt gattgaatct
    16921 gaaagcggaa caattattga aaaaggataa gttgttttac taataagatg tattctattt
    16981 ctagtatctt tatatcaaat tagaaattat tcttgaaaac atgtacaata tttcaaaaga
    17041 tactagtttt agactttaat atggtaaaac aaatataaat agaaagcgtt ttcttgaatg
    17101 tttatttaag aaagtagttg gttttttaca ctttgttaga catcaggagg aaaaacaaat
    17161 gagtgaaaaa gctaaaaaag ggtttaagat gccttcatct tacaccgtat tattgataat
    17221 cattgctatt atggcagtgc taacttggtt tatccctgcg ggggccttta tagaaggtat
    17281 ttacgagact cagcctcaaa atccacaagg gatttgggat gtcctcatgg caccgattcg
    17341 ggctatgcta ggtactcatc cagaggaagg ttcgctcatt aaagaaacga gcgcagcgat
    17401 tgatgtagcc ttcttcatcc ttatggttgg gggtttcctt ggcattgtca acaaaactgg
    17461 tgctcttgac gtagggattg cctctatcgt gaagaagtat aagggccgcg aaaaaatgtt
    17521 aattttggta ctgatgcctt tgtttgccct cggtggtaca acttatggta tgggggaaga
    17581 aacaatggcc ttctatccac tccttgtgcc agttatgatg gccgttggtt ttgatagcct
    17641 gactggtgtt gcaattattt tgctcggttc tcaaatcggc tgtttggcat ctactctgaa
    17701 tccatttgcg acaggtattg cttcagcgac tgcgggagtt ggtacagggg acggtatcgt
    17761 acttcgtctg atcttctggg ttaccttgac tgctcttagt acttggtttg tttaccgtta
    17821 tgcggataag attcaaaaag atccgactaa gtcactggtt tatagtactc gcaaagaaga
    17881 tttgaaacac tttaacgtag aagaatcttc atctgtagaa tctacactta gcagcaaaca
    17941 aaaatcagtt ctcttcttat ttgtgttgac attcatcttg atggtattga gcttcattcc
    18001 atggacagac cttggcgtta ccatttttga tgactttaat gcttggttga ctggtcttcc
    18061 agttattggt aatattgtcg gttcatctac ttctgcacta ggtacttggt acttcccaga
    18121 aggcgcaatg ctctttgcct ttatgggtat cctgattggt gttatttatg gtcttaaaga
    18181 agataagatt atctcttcct tcatgaatgg tgctgctgac ttgctcagtg ttgccttgat
    18241 cgtagcgatt gctcgtggta ttcaagttat catgaacgac ggtatgatta ccgatacaat
    18301 cctcaactgg ggtaaagaag gcttgagcgg tctatcttca caagtcttta tcgttgtaac
    18361 ttatatcttc tatctaccta tgtcattctt gatcccatct tcatctggtc ttgccagcgc
    18421 aactatgggt atcatggctc cacttggaga atttgtaaat gtccgtccta gcttgattat
    18481 cactgcttac caatctgctt caggtgtctt gaacttgatt gcaccaacat ctggtattgt
    18541 gatgggagct cttgcacttg gacgtatcaa cattggtact tggtggaaat tcatgggcaa
    18601 actcgtagtc gctattattg tagtgaccat cgcccttctt ctccttggaa ccttccttcc
    18661 attcctataa aatagtgagt gaggtgattc catgaaaata gatataacaa atcaagttaa
    18721 agatgaattt cttatatcat taaaaacctt gatttcctat ccttcagtac tcaatgaagg
    18781 agaaaatgga acaccttttg gacaagcaat ccaagatgtc ctagaaaaaa ctttagagat
    18841 ttgtcgagac ataggtttca ctacctatct tgaccctaaa ggttattacg gatatgcaga
    18901 aatcggtcag ggagcagagc ttctggccat tctctgtcat ttggatgttg ttccatcagg
    18961 tgatgaagca gattggcaga caccgccatt tgaagcaact atcaaagacg gctgggtatt
    19021 cggacgtggt gtccaagatg ataaaggtcc ttcgcttgca gctctctatg cagtaaaaag
    19081 cttgctggac caaggtattc agttcaaaaa gcgcgtacgc tttatctttg gtaccgatga
    19141 ggaaaccctc tggcgctgca tggcacgcta caataccatc gaagaacagg ccagcatggg
    19201 ctttgcacct gactcatctt ttcctctgac ctatgctgaa aaagggcttc tacaggtcaa
    19261 acttcatggc cctggatcgg atcaactaga gcttgaagta ggaggcgcct ttaacgttgt
    19321 accagacaag gccaactacc aaggtcccct ctatgaacag gtttgtaacg atctcaaaga
    19381 agctggttat gattaccaat ccactgaaca aaccgtaacg gttctcggag tgccaaagca
    19441 tgctaaggat gctagtcaag gtatcaatgc tgtcatccga ctagctacca ttcttgctcc
    19501 tctccaagaa caccctgctc tcagctttct tgcaacacaa gcaggtcaag acggcacagg
    19561 aagacaaatc tttggtgata tagcagatga accttctggt cacctatcct ttaatgtcgc
    19621 aggtctcatg atcaatcatg aacgttctga aatccgtatt gacattcgga ctcctgtctt
    19681 agctgacaag gaagaactag taaagttgct tacaagatgt gcacaaaact accaactccg
    19741 ctacgaagag tttgactatc tagcgcctct atacgtcgca aaagacagta aactcgttag
    19801 cacactgatg caaatctacc aagaaaagac tggcgataac agtcctgcta tttcatccgg
    19861 tggtgccact tttgctcgca ccatgccaaa ttgtgtagcc ttcggcgcct tattcccagg
    19921 agcgaagcag acagaacatc aggcaaatga atgtgccgtt ctagaagatt tgtaccgtgc
    19981 tatggatatt tatgccgaag ccgtctatcg acttgcaact taatcaggca actgtttcta
    20041 caaaaaaaaa tcgaccgatt aatgaactgc accccaaaag ttagacagaa taaatctaac
    20101 ttttggggtg ttttattatg aaattgagtt atgaagataa agttcagatc tatgaactaa
    20161 gaaagcaagg acaaagcttc aaacagcttt caaaaagatt tggtgtggat gtttctggtc
    20221 taaagtcatc tgaatctttg agatgagctt tataaatcgc ttttttcagt ttttgcactg
    20281 gtatttcgat aaactcaaac tttttagccg ttgtattgcc tgattttata gtatgttgaa
    20341 actagaatag tacacctcta cttctaaaac atttttagaa atcaatttga ctgtcctgat
    20401 tgatttgtcc tgttcttatt tcattttact atatttgagc cacttcgtct ttaacggctt
    20461 tattcataag ctcttgtaat ttttctttac tatcaattac ttctgatttt ccgttgtaat
    20521 ttattgtaat aggttttaac ttacctaatt tctcgacacg ctcattaatt tgatcttttt
    20581 tgaaggctgc atatgttttt cctaatattt tttcgaaaat atatttatca gatagcggtt
    20641 tgtcttcttc ttcagcttgg tttttgtatt aatttgaaac ataaggaaca aatccttcat
    20701 agtaacctaa tgctcccata agttcaaaag cttgttttct aattcaagcc attgcaactc
    20761 agatttcagc ttttcagata aatcctgctc atccaaataa tgacttaaaa ttagtgctga
    20821 actcgtttct gtatcctgta caggctgatc acccatacca tcaaaaaata aactcgttcc
    20881 tagcaagacc gaacaagctc ctattgcata tggcctcaaa gaaaaacgct gctttctctc
    20941 aaattgaaat tctttcatcc catctcccat cattcattat tactgtatat tttgtatatc
    21001 agaaatagtt tgtattcaca aatctttcta gttattccct tatcattcct aattaagaga
    21061 gataacatac aataattttt agttaaatgt atatcgatgt tttttgtttt tcttaataaa
    21121 cgcaatacaa aaagagcctg ttgccaagct ctttgtactc aatgaaaatc aaagagcaaa
    21181 ctaggaaact agccacaggt tgctcaaaac accgttttga ggtagtagat aagactgacg
    21241 aagtcagctc aaaacaccgt tttaaggtag tagataagac tgacgaagtc agctcaaaac
    21301 actgttttga ggttgcagat agaactgacg aagtcagctc aaaacaccgt tttaaggtag
    21361 tagataagac tgacgaagtc agctcaaaac actgttttga ggttgcagat agaactgacg
    21421 aagtcagctc aaaacactgt tttgaggttg cagatagaac tgacgaagtc agtaacatct
    21481 atacggcaag gcgacgctga cgtggtttga agagattttc gaagagtatt agtctattat
    21541 ttcttctcag cgcgaagggc tgacaagatt tgtgttcgga tatcatccac accatttggc
    21601 gtatttggta aaaagatggt ttgatttcct ttagaggcaa aggtattcaa ggtatccaaa
    21661 tactggttag tcaagaggat agacatgatt tgttcttctg tcatgccaac attggcttcc
    21721 ttgagttcgg tgatagactc tgccaatcca tccacaatcg ccttacgttg ttgggcaatc
    21781 cccacaccat gaaggcggtc tttttctgct tcggcttcag ctgcagtgac aattttaatc
    21841 ttgtcagctt ccgccaattc ttgtgctgcg acccgcttac gttgcgccgc attgatttca
    21901 ttcatggatt gcttaacttc tgcatccggt tcgactttgg taatcaaggt tttcacgata
    21961 atgtagccgt aagtggtcat ttcttctgct acttggtgtt gaacctcaag ggcaatctca
    22021 tcttttttct caaacaattc atccaaggtt aattttggaa cagaagagcg aagagcatct
    22081 tcgatataag atttaatctg agattctgga cgtatgagtt tatagtaagc atctgtcacg
    22141 ctctgctcgt tgacacggta ctgagtcgct acattcatca taacgaacac attgtccttg
    22201 gtcttagtct caaccacaat atcactttgc aacaagcgca actgaatccg tgctgcaatc
    22261 gagtcaatcc caaaaggcaa gcgaatatga ataccgctat tagcaacctt ttggtatttc
    22321 ccaaagcgtt caataatcgc caccgactgc tgacgaacca cataaactgt actcagtgtg
    22381 actatcacca ataggagcac acaaacaatc agaaaaatca tgaaaaatat tgccataatg
    22441 gaacctccac aagtattttt ctagtattat agcacattta aagaaggctg tgccgttttt
    22501 actgcgattt ttcctgaaat gtcaataatt agaggtgaat tgtcctattg tcgtctaatc
    22561 tcttgctaaa ataactcttt ataaaaggca atcgtttctt ctaaggttgg cataaatgga
    22621 tttcctggtg cgcaggcatc aatcaaggca ttcttagaaa ggtattcaaa gtcgaaatct
    22681 ttttcttcaa taccaagttc agtcaatttc ttaggaatac ctactgtctc agaaagcttc
    22741 tcaatctcag caatcgcata atcggcacat tcttgatctg atttaccttc tacatgaagt
    22801 cccaaggctt tggcaacatt gcggaaagct tctggtacac gtttagcatt ttcacgttct
    22861 ataactggta gcaacatggc acagcaaaca ccatgtggca aattatatac tgcaccgagt
    22921 tggtgagcca ttgagtgaac ataaccaaga ccagcattat taaagctcat accaccaagg
    22981 aagatggcat tgaccatacc ttcacgcgct tcaatatcat gtccattttc tacagcacga
    23041 ggaagatatt ccttgataag ctcaatagca ccaatagaca gtttcttggt tacatcataa
    23101 gcaccaggtg ttaccaaagc ttcaacagcg tgagtcagag catccatacc agtagcagca
    23161 gtcaggcctt taggtttgga aagcatgagt tccgggtcat taacagagat aagagcaagg
    23221 ctattcttgt caaccattac catcttaacc ttgcgttctt catcagtaat cacatagtta
    23281 atagtgattt ctgcagaagt tcctgccgtt gtattgatag ccacaactgg caagcctttt
    23341 ttagcagact tatgaagccc ttcataatcc tgtggttttc caccatttgt agccatgata
    23401 gaaatacaac tagctgcatc ctgtggagaa cccccgccaa gactgatgat aaagtcacag
    23461 ccatgttctt gcaaagcagc taccccatct gtgacatttt tacaagtagg gtttggctct
    23521 acatcgctaa agatgacata ttcgattcct tctgtatcca gtggttttaa agtcttaggt
    23581 aaaatatcac tgccttcgat gtacttatct gtcaccaaaa gtgccttttt atagccaagt
    23641 tccttgatat aaggacccac ttcatttaca acaccttttc caataaggtt gactgccgga
    23701 acataaaatg tagccatata attttcctcc tgcgcctccg cgctatctat ttaacatcat
    23761 tatattactt tttgtattcg tttttaatta tttgtgaatt gtttaacaaa tctttttaaa
    23821 aggataggag ggacgtaagt tcacctccta tataaacaag ttttatttat gcagtggtcc
    23881 caacaactga catgcacgat agtctgctga ttctagatct tctgttccaa ataaggacca
    23941 atttttaggt ctaaagatat cttcctcagg tacattatgc atattgacag gaattctcaa
    24001 catagaagcc aaggtaatca agtctgctcc aatgtgtcca tatgttatgg ctccgtgatt
    24061 agctccccaa ttattcatga cgtcatagac agacttgaaa gcaccttttc ctgtcaaacg
    24121 tggagcaaac caagtagttg gccatcctgg atctgtacga ttatctaaag tatggtgaac
    24181 atcttcagga agttcaagtg tataaccttc tgcaatttgt agcactggac caaccccttt
    24241 tagaagattg agacgtacca ttgttactgg catatccccc ttcgtcaaga aacgagttga
    24301 gactcctcct ccacggaagt attcgcggtt tgctggtggg aagtctgtat tttcaagcat
    24361 agcctgcact tcactttctt ccaactccca gaagggtttc ataacaggtt tgccatctcg
    24421 agtagcttga cctgtaccat ccaatgtaca agaaccagag ttgattagat gtaagaagcc
    24481 agctgcagca cgaccctcta aagtatgtct cgttacacgt ttaacagcct ctggactcca
    24541 ataagtacgc acatcagcaa agatttgtgg agtatttgtt aatagataat taaagagcat
    24601 agacacacca tttagtgaat cattctctgt cgcaaataca aatggttttc gaataccatt
    24661 ccagtcaaac tgagtattga ggaaagtttc cataaagtcc ccatttggaa aatggtctgt
    24721 ccactggcgt tgaccttgga aaccagctac taaagcatgg tgaccaactg cttcttcttc
    24781 aaaaccaagt tcagcaagtc ttgggttacc aaccattaag tcacgtccaa tcatgaacat
    24841 cttaataaca aattcccatt gtctatcttt ttcttcacgg cttaaaacaa ggtcttcacg
    24901 gttatggtcg aatccttctt ttacgttttc tttcacccat ttgagcgcac gttcaaactc
    24961 ttctgggtca taaataccac ggtccatacg gcgcgtgaac tccgtcatat ctaccgattc
    25021 atttcgcatt cctaagtatt cttggaagaa atctggattt acaatagaac cgccaatccc
    25081 catcgaaaca ctacccattg ataggtaagc agtgtctctc atcaagccag ttgcaagagc
    25141 tgcacgcgca taacgtaaaa gtttttcttt gacatcttct ggaatatctg tatcattagc
    25201 ttcctgaaca tctctaccat aaatcccaaa ggctggaatt cctttttgag catgtgaagc
    25261 tagtacagct gcaagataga cagctcctgg gcgttctgtc ccattaaatc cccaaatagc
    25321 atgaggaata tctggagaca tatccatagt ttcactacca taacaccagc atggtgtaac
    25381 tgtaattgtt gcgcaaacat ttgacttttt aaacaactca tgggaagtcg cagcctctgg
    25441 aacacgacca atggtagatg gagaaatcac gcattccaca ggttccccat ctggatattt
    25501 caatgtgctt gaaatcaaat ctgccacact tttagccatg ttcattgttt gcacttcaag
    25561 tgattcgcgt acaccttgac gacgaccatc aatagtcgga cgaataccaa tacgtggatg
    25621 ttgaatcata ctattttcct cttatttttc tgtttctttc atactaccat ctccattata
    25681 tttacggtag cctgggtgac gacctgtaac cttataattt tctcgcattg agaatagacg
    25741 ttctaaagtc ggacgagcaa tttcttgctc ctcaatgccc tttgtagaaa gtaacattct
    25801 tccgtggaag gttgtctttg cgactaattc taaagtttcc atacggtagt atgctgtaat
    25861 gacatcgctt ccgacagtca gagctccatg attttctaat agcatgacat catgatcggg
    25921 cagataaggt gtaattgctt ctggcacttc cattgtagac ggtactccaa atggggtaat
    25981 aggaattgcc ccaaccacaa tcgcgctctc aattagtgaa taagtatcta aaggaatatg
    26041 tgcaagagca aatcctgttg caatcggtgg atgggcatga acaaccgcac ggacatcctc
    26101 acgttcttcg taacaacgaa tgtgcatttt aatttcacta gaaggacagt aatcaccttc
    26161 tgcttctaaa atctctcctt taagatttaa cttcaccagc ttttctggtg taataaaact
    26221 tttgctgata ccagtaggtg ttgcaagaat ggtatcctca tctaatcgaa cagatacatt
    26281 cccatcgtta gcagcaaccc aaccaagttg ccacatctta tgacaaacat cacaaatctg
    26341 ttcacgaatt aatttttcat cttgagacat aaactcactc ctttctaaaa atgaagttcg
    26401 aattcaagat ttccgttact gttaatggta atagtaacca tatgattttt ctgatcaaaa
    26461 ttgagagatg gctgatcata ggaattattt gttcctgata atgaatgaat agtaggaagt
    26521 ttatctatac ctcttaatac tatctttgta acccttttct ctccaaactg agtatctttg
    26581 atataatgtt cttcaatcca cttaatcgca cctttcttgg tcaattgtgg tagtttcttt
    26641 gcctgatttg catcttgagc attagaccat agtgaatctt tatttgttct aaaattattt
    26701 aaattcactt tcaatccatt acttattctc tcaaaaatac cataggtatg aggttcaaat
    26761 tcaacatcca tttctaaaga attaaatttt aatttcccag tctgtttaac attctcattt
    26821 accttgtaat tatagacaaa ccatctatta tctaatttct gagcaaagat atctccttca
    26881 taattcatag gatacagctt atttaagtat tcttttctag cctgagtaga gctaaattct
    26941 ggagaagtga tttccttaat ctgaatacgg ctaccagaaa cagactcttt caatttatct
    27001 gtttttaaaa cacctggaat agctggaata acattataac gaccagtcat gtataatgga
    27061 gtttgttctc tatcagtatt tacgtttaca aagaacttac catttccttt atttgaatag
    27121 tctccatgta tgaatacttt tgtatcctca agtacctttt ctttggatgg tgctggatgc
    27181 gctattacat atctaaaaaa ttcttttatt acttcgctaa agagtaagct ttctttatta
    27241 tttacaccat aggtataagc tgggtgttca aaattataaa ctacacctcc attaaggtaa
    27301 acccctaaag cttcttctcc tagcatagac tctggttcag tcaaccattg tctatctcct
    27361 tgactttttc cgatattacc acttgcaaac agtttccatt tacctgtctc ataccacttc
    27421 caagtatcca tcaaaccacc ccattgataa gtatgatttg aaagccaaag tcccttcata
    27481 taactttctg tagttgaatc atttccctca gcagcaggtg tatttttgaa catgaaaata
    27541 aggttcttcc agtacttatc aacagatttc tgaaatgcta tcttaccgtt ttttccaaat
    27601 gctttttcta ttgcagaacc attattttgt tcagcccata taaaatatcc tccattttta
    27661 gctgatactt ttaagtaatc tgccgcctta ttttcaatat catttgccca aatccaataa
    27721 ttctctgtac tgaaaatacc atgcaaattt ggatattttt gatacatttt atcaatccag
    27781 ctggtactta accaatgtgc cgaagtataa taaggcatat taccagcagt gtaaaccgtc
    27841 agaataactg gaattggttc attttttcca gtcttaggat ttacatattt cgctgcttct
    27901 tctaacatat gttcataaaa atctctagca gctccatctc ttttaggaag atcatctgga
    27961 tgtaactgta taacagtata tggttttaag tcatctggta tattctccca cttacctgtt
    28021 aatgtatctc ctccccaaaa agtatatttc ccttggttaa actcttctcc gttagcatat
    28081 aaaggcatca taagtaacgg atgttcatta ttaatttcac gacgtttatc aacttctgac
    28141 caatcattag gattcttaaa tttaccattt gccataaaca aggctccacc taaaaccaat
    28201 tcgtcagccc atgtatgttt ccctgaatat gatttaagtt ctattgtctg tgtatctttg
    28261 ggaatttcaa catcaaccag aaatgccgat gtatcatact taattccttt tggatatttt
    28321 gaatctgatg agtaaatgac tttaccatcc gcaacaactt caaatttttg aatatctgcg
    28381 taatctgaat ttaaatgatt tgcactccta tcaattccga cataggaaat aaatcttgta
    28441 acattagcac ccgaaacatc atattttata ctagacgggt tgcttgctac agttcccaat
    28501 cccttattaa attcgacttc cttaccgtca attagtaatt ttattttatt attagacccg
    28561 ttattacctg gtgtaaaagg cttatctttc tgaacagttt ttgatttatc gtcatcacca
    28621 tgagtagcat caacccattc taaatcagaa agatatactc ctccattaat tcctttatta
    28681 agaagatctg gagttatagt ttgagtacta acagttttaa taagtttagc atcagcaaat
    28741 acaacctcat caccccatgt atgttctcct gcgaatgatt ttaaggatat ttttttagcg
    28801 ttttgtggaa ttgtcacact aataaattgt gcctgggtat tatatctgaa tccttctgga
    28861 ttagttacac tactagaata gacaaccttt ccatcaattt ctatttctat tctatccact
    28921 actgcatggt ctgatctaga actattagca ctttgatcaa ttcctatata agtttcaaat
    28981 ttttcaaacc cttgaccgct gatatcatag ctgattactg atggagaatc tgcgactgtt
    29041 cctattcctt tatcaaaata tttaacttta tcatcagaag tgagcaatga aatcttagta
    29101 tgctctcctt tatttcctgt tgtaaatgga gcatcttttt gtactgtttt tgttttatct
    29161 atatcaccat gagttgcaga agaccaatcc atatctgaaa gatatgtcat ctctttatca
    29221 atttctttat ctttagtaat aagtttttct aagttattcc ctgttactct agcagtttca
    29281 gtactagctc tgttattaga attactttga actacatctg cataaaccaa attacttcct
    29341 aaaaaacttg ttaaaacagc cgtagctatt aaaatctgtt ttgctatttt tttactttcc
    29401 atttttattt cctcaatatt tacatacaac ttcttcaata acaaataatt ttgtaagata
    29461 ttctggaaca caaagtgcta aattacttaa actgactttt attccagcat tcattaattc
    29521 atctccataa aagaactcat caaatctaga gacggaatag catttatctt tatctaaacc
    29581 actaaaataa actcttgtat aacctggatt caacttacta ttcaattgag catacaataa
    29641 aatcgattgg catttatctt ttgaaacaac ttgccaacta gcagaatcac aactaaaagg
    29701 actgtttatc ctatagaaat ccccatattg aaacaatgaa cgatattttt tataaaactg
    29761 aatttgttct ctcacttgtt ctttttcttc taccgacaat ctcgtaacat ctaattcata
    29821 accgaaagaa ctaaaataag ctacatttgt cctagtgtcc aaactcgttt gtcttccact
    29881 ttgttcattt ggagaaatag aaacatgggc tgtcatcatt gatggagaat atccataaga
    29941 agttccatgc tgaattttca atctttctat cgggtccgta tcatcactcg tccacgcttg
    30001 cggtgcataa tacataagtc ctaaatcaaa acgtccacca cctgaagaac aagattcaaa
    30061 tagaactgaa gggaacttag ttattaaacg atctaataac tgataaagac ctaagatata
    30121 tcgatgacca aattccatct gctgttcaga atcaagttca atactataaa tatctgttat
    30181 atgacgattc atatcccatt tgatataatc cagatttgtt ttgattatga ttttagaaat
    30241 agaatcaaaa aggtaatcaa caacttcctg tctcgtcaaa tccaacacat attgatttct
    30301 tcctactgac ttctctctgt ctaacaaatg gatagtccaa tcggcatgat tcttgtacaa
    30361 atcactatca atagaaatca tttcaggctc aaaccacaaa ccaaattgta aacctatttt
    30421 gtgaatttca tctgcaagaa atccaatacc ttcaggaagg cgacttctat ctgttaccca
    30481 atcacccaga gaacttttgt catctttcct atgaccaaac caaccatcat ccagtacaaa
    30541 taattctatc cctaaatctt tagcagcctt tgctaaatct aaaatttttt ctgtactcaa
    30601 gtcaaaataa aatgtttccc aactattgag tattatagga cgactagctt gagaaaaatt
    30661 aggattaact aaatgatgtc gataaaaatc tgatagttgt tggctcatct gattcatccc
    30721 ttgatcagaa tagatcacaa ttgcttctgg tgtcacaaaa ctttcggcag gatctaataa
    30781 aaaacgaaat tctactggat ttattcctac cgttactcta gcggtgtcaa aatggtcaac
    30841 ttcaatcata ttttgaaaat ttccagaata gataaagtta aaaccataaa cctcaccact
    30901 atgctctgta gtttccctcc ttgatagcat aaagaaagga ttttgctgag gacttgatga
    30961 gtatctcaag ctatctattg cttgaatacc tggtctaagc gaagttctat ataactgacg
    31021 ttccctcagc catgctccag aaaattgaat aaagtcatat tgactatcag gtagatctag
    31081 ctgtaagctc attgctttca aaagagtcaa cttattattg ctattattta aaaattccgt
    31141 atttctaact aagacattgt attccggaaa aattgtgtaa tttagtctaa cagttaaatc
    31201 taatatggaa tctgttaaaa taatcgttaa tgtttccgca cactgaccaa tatcatcaaa
    31261 tgttgaaggt agagagttaa gacgattttt ccctttttca agttcaaaac cttgatattt
    31321 tagtgtcgtt atcctactac cattcattcc ttgaacttga aaggctggat gccgaaaatc
    31381 tcctttccca tatgaagaat attcattttg tatcatagac tgttgaatat cctcaaattc
    31441 ttcagaaaaa gatacttcaa atcctctttt ttcataggcc atcaattggt tataattttc
    31501 cttgacatga atccttttcc caaaatgtag atgttgaagt tccccatttt ctaaaattct
    31561 aaaaatataa ctactatctt tcattgccaa atgaaaaacg gtctcgtcct tattaatata
    31621 aatcgtcatc tctacctcac aattttttct ttacttcttc agtatcacga tagagtacgg
    31681 tgccaattct atctcatctt cttgaatagt aaaatctcca attactctgg taaaattctc
    31741 tatcgaaaga ttctctattt ttattttttg tttaatctga gcaggatttc tcagagagag
    31801 aatttcagta gaagtcttct gattgtgaca cttataacca taaatttcaa atttttcagg
    31861 acttcctcct atttttttac tatattttag tgtttgataa ttctcctcta tccatttgac
    31921 ggcacgagca ttggctttcc agcgttcttc atcaaacata tcaaaagaat agtgaaactc
    31981 ccaaaaagca ttccctcttg ttgaaataaa taatagataa gcttcaaaat ctggtattga
    32041 tgcaaacatt tgatgatcca tgtaccaggt atttgcactg acagcataga ttggttcatg
    32101 attataaagc gaccacatag gtaactgtat ctcacgtttt tccaaaaatt cttgatactg
    32161 aatatctcgg taagttatca tacgattgat atcattacct gcattctctg taaagcctac
    32221 atcttgagat atttgaatcc ataaactatt gacccactgt aaaaaccatg gactaggatt
    32281 tacataagaa gtcaagttta accaacaatc ttttcctcct ctctcctttc ttagatctat
    32341 caacagttga attaagaact cataaaccac tgtcatggta tacataccgt gcggtccact
    32401 cttatcaggt ttgtcaggtt gaagtaacca gccatcaatt ttccaatagc tgatatcgaa
    32461 ttctttttga tattccaata tccttttctt catttgattg agatagttaa aatcagccac
    32521 gtttacatca tttgaaatca aatttttaga tccaatattt aactctggat gtgcttctaa
    32581 ccaatcactc ataatgattt ctgtcccatt ataaccacct cggggaccaa tccacaatcc
    32641 taggcttgaa ccaaatccat ttacaagatg tttaatattt ctcaaaccat ttgggaattt
    32701 atgattgaat tcccaaactg attgatagtt tgtccaacca tcatcaacaa cataagcatc
    32761 taaatgaact ccatgatttt caaatccatc tcgaatctca gaaaaacttt ttaaaatacc
    32821 ttcctctgta atatcggtca tatggtcata ccaggaatta tactgtttac ggaaataact
    32881 aggttgagct attccttcaa catattcaaa aaatgcctct tgaatctctt cttttttata
    32941 agaacatgct cccccaataa tacaagacca caaactcttt tcttgattta caacagttcc
    33001 caagtaatat cttgatacat agtgtctacc atctaccttg ttttcagaca ttggaaattc
    33061 cattcctaag aataaagaat tcgcataaac aggttgtccc aattctacat aataaccgga
    33121 aaaattcgcc atttccttaa tacaatcttg tcttttcgga tagaaaatat tgtcttctgt
    33181 ttcaaactca aaactctcta cctctacaga tttaataacc tcagtagatg attctaaaac
    33241 taattgcttc gaaagaacat catccctcga ttcaaagatt aaacgtaaac gaatggaagg
    33301 acaaacaaaa gaaacctgaa ttcgcgtatc tccctcttcc ctaacgattg ctgaaaaatg
    33361 atttgaagaa agtcttatac cattctccaa tgtaatggta aattcttctt tcgaaacatt
    33421 ttttatcatt actcctgccc gtttgtttac gatatcagta gtataaaatc gaccatctcc
    33481 ccaaaagaaa ttacgtctta catttttatt ttcaattttc atataaacta ctctctcaac
    33541 tcaattttga ttacgctatc aatcaagtct ggtaatggat aggtaaaatg tggaacttct
    33601 ccaaactgtg caaaacaaat tcctttgtag gcattggtcg tccagctttc tgaaattttc
    33661 acctcacttc catcatgaag aaagctcatt ctttttacgt tttctttact aataccaaga
    33721 agagctaaag gacctatagg ttgttcaaat acatgagcat aaaccgtatt tccattacga
    33781 gtatagtatc cccaatctgg ttttggtaaa ttttcatctc cactacatcc ataaattgcc
    33841 tcaccatttc gagacatcca tcgatgaaaa ttatctaata ttttcttact tgaatcatta
    33901 attcttccca aagcatctgg gcctacatta agaatcatat ttccgttttt actaacacat
    33961 tccactaact ttcttatcaa agtttgacta gacttatata gataatctgt aggattataa
    34021 gcccaattgt tattcatagt tagacaaagt tcccaaggaa caggttcacc tttaaagttt
    34081 cgaatacctt cgtgaggaac aatttgctct ggactaacaa aatctccagc gtaagaggta
    34141 atttcatctg taacgatact tccgaatcct tcacctgatg tttctaatct attatctacg
    34201 ataacgtttg gctgataatg acgaaccatg tctattaatt ttgaagctcc ccatttttca
    34261 cccaccatgt cttcataaga ataatcaaac cataatatgt caatctgacc ataaccagtt
    34321 acaatttctt taacttgatt gtgtaaatac tccaaataac tgtcaaagtt aattttctca
    34381 tctcgatagg cttcatttcc tctcattgga tgatttagat cagcatattt agggaaatca
    34441 ggatggtgcc agtcaatcaa actaaaatat aagcctactt ttaaaccttc agctcgaaca
    34501 gcatctacaa attctttaac taagtctcta cctgcaggtg tatttgttgc tttataatct
    34561 gtaaattttg aatcaaacaa acaaaaccca tcatgatgtt tagccgttaa aaccatatac
    34621 ttcatccctg cagcttttgc ttgtttagcc cattctctag ggttatattc cttaggatca
    34681 aaagctcgaa aatatggttc ataatcttca atagataatt tctgatgact acgaatccat
    34741 tccccttttc ctggaataga atataacccc caatgaatga acatcccaaa tcttgcttct
    34801 ctaaaccact gagttctact attgatagtt tcaagtgtat gtttcatcct tttactgctc
    34861 ctcctcctaa agcttcaatg aagtatttac ttgtaaatgc gaatagaatg attggtggta
    34921 atacgattac aacaatagct gaaaatagac cattataatt agtattaaaa cttgtagtaa
    34981 attgacctag taatgctggt actgttaatt catttttatc aatcaccaac atagatgccc
    35041 aataatattc attccagtta ttaataaagg caagtactgc tgctgttaaa attcctggtc
    35101 tagcaattgg caacatcact ttccaaaaag tttgagcata tgtcgcacca tcaatctctg
    35161 ctgcctcctc caattctttt ggaataattt caaaatagtt ctttataata aagaaactca
    35221 tagcaattcc actacaagag tagaccagaa ttagagcaac tggggtatta taaatcccca
    35281 actcatttac taatctataa actggaaaag tcatagctgt tgctggaata aatagagtag
    35341 aatacagcat agaagtcacc aacttcttcc ctctaaattc catcctagca ataacatatg
    35401 aagacatgga gatcataaca atgctaataa taactgatat agacactact ttaaagctat
    35461 tccaaaagta agtaaaaaca tgaagttttg taaaaacatt tatataacct tcaagagtaa
    35521 agtcagtcgg taaactaata cctggatttt tcataggatc tgcttttaat gatgataaaa
    35581 aaatccacgc aatagggaaa atagaaatta aaatggtaag gagcatataa ccatatataa
    35641 gaactttgcc ttttttacta ataccattta tatgtgtatt cataatcatt tcccaccttt
    35701 cagtcttttt ctttctctga ataaaatatt aatacaaacg ataatcaaca ttccacctaa
    35761 gatttgtata actccaattg tatttgcacg agcatactga gttcgtgagc tagccactgc
    35821 taattctcga ataacaaaac tgatactttt tgttcctgca gcaccatttg tcaaaaagaa
    35881 tacttcgtta tagagcaaaa atcctgaagt tgctgccata attgaaacag ttttaattgt
    35941 ccccttaatc atcggaatcg taatatacca ctctttcctc caaccagtaa tattgtctaa
    36001 aatagcagct tcttgaactt cttctggaat agcaaaaatt tgcccaagaa tcataatggt
    36061 tgatgctcct gcaaagaaga tataagcgcc agtcatagct actatattta atcctggagt
    36121 caacagtact gaatctttaa acgatggatt aaatagatga ataatttggt taacaacacc
    36181 atagtttgga ttatagattt gaagaaaaat caagcccata gcagcactag aaatgatact
    36241 tggtacaata taaagattcc tagatatctt ccagcctttt aatttcttag ataatgtaat
    36301 tgcaacagat acagctaaag gaacttgaat aataacacca attacagccc aacgaacaga
    36361 attaattaga gcttctgtaa aaaatgggta ttctttaaaa acatacctat aattttcaaa
    36421 taatttatca aatccaagaa actcaggggt gtttaaattt gtataatccc atttagcaaa
    36481 tgaagttaaa aagattgtta ctacagaata aaaataaaat agaaagaaac aaatcaacgt
    36541 tggcaaaaga aataaaaaga taaatcgtct tttttgattc ttcctatttt gtaggctcat
    36601 aattttttgt gccataaaca aactcctttt tttcaactat ataagtaaaa tcaagctcag
    36661 ttggcagtgc caactgagct tcgttttcac tccctaaaac aattaaccaa tcaatgattt
    36721 caacacatct tgagtagatt ttactttttg atcaacatct acattttcag cagcactttc
    36781 tgtcaatgca ttgataatag ctgtgtgtac atcgccaccc caaacatcac ttacagttgg
    36841 aacaactgct tttgcattct taacttgagt gattgcttgt cctaaaattt tagtagtagc
    36901 ttctgaactc ttttctgaaa gttcttttac atttacattt tcattagaag gatttgctcc
    36961 tactttttca aaaattactt tttgtacatc atcgctagtc atatatttca aaaactcaag
    37021 agctagtttt tgtttagctt cagacatctt acttgagata gtgataccac ctccagaaga
    37081 actaattgct acacctgcag gatagattcc aggtgcaaga cttgggttct tagacatctc
    37141 accagaagcc cacacaccat taaagaatac tgcagattta ccttcttgga aatctttgga
    37201 ataagcattg gcatcgccac cagcattctt agaaccatta gcttgaattt ctttcataac
    37261 cattttcaaa gcatctgcaa attctttaga ttcaattcct tcttttgtta atggtttatc
    37321 taataattta cgtccatttt ctgtggttcc taatactgta ttaaacaagc gaatagatgg
    37381 ttctccagca ccaaatgcat acactccatc ttgttttcta atgcttgcca ttgcctgagt
    37441 gaaatcatcc caagtattcc actgatcagg tgtcttagca cccgcttttg caaatatgtc
    37501 cttgttgtac cacaaaccca ttgtaaataa ctgttcgtga actgtataaa tttttccatc
    37561 tttttggttt tgcttatagt tcaaaccaac acttttttca agcttatttg aatcgatata
    37621 tggtttcaaa tctaaaacaa gtttttgttc aatcgcagct aatgatactg cattacctgc
    37681 taggtcaatc acatcaggaa attcaccact agcaaccttg ttattcatta tatccttcaa
    37741 atcaccattt acaggagttg gtttaaattc tacaccacta tctttatgtt cttctgcaaa
    37801 tttatcatat aaatctcgca tagtcttcgc tacaggccat tcatcttcac tatgatgata
    37861 cccatgataa aattctaata cttctttacc agaagaactt ccaccgcttg catccttaga
    37921 tgttccacaa gctgctaaac caaaggtagc tactaacccg ataacagcgc atttcaaaag
    37981 actttttttg ttcatgacaa aacctccgtt tttttatttt tgtttatgat agctgaggat
    38041 aataatattg tgtatcagca atatttgtca taatatcctt taactctgta tcctcgttta
    38101 ttttaccttg actcaatagc tgcacaacaa tattccccac agctgtaccc tcagtcgaac
    38161 ctgtaagaac ctctttacca gttctatcag caatcatttg gttaaagtaa ctggctctag
    38221 cacctcctcc aatcacatat atcctcttat aaaccttatg agttagttct tctagtaact
    38281 ctatcgcttt cctatacgtt tcagctaggc tttcataaac aatcttaaat agttgtccat
    38341 ctgtccactc tctagtttca tgatgataag ctagatattc tgtcaaagtc ttgtgcatat
    38401 cagattctgt tgcaaattca gttgattcag tatcaatcag aggaagattt tctttttctt
    38461 tctccaccat tgtcctaata tcatcaaaag aataggcttt ccctcttcgt tcaaatgaac
    38521 gtcttagttc ctctatgatc cacaaccctg tacaattctt cagaaatgta atcactccat
    38581 ctttaccgac ttcatttgta aatccataac tgaaggattc ggtagtaaga atcggtgaag
    38641 taagttccac tccaaccaaa gaccaagtac ctgatgaaat aaataaacta ccttctgtct
    38701 taggtactga gacaatcgca cttgctgtat catgactaca aacattcaca acaggaatat
    38761 cgcctaaacc atactccttt tttatccttc caagaacatt tccctctgaa acaatttcag
    38821 gaagtaaaga tgaatccaat tcaaatagtt ttaagatatt ctgattccaa ttttgactcc
    38881 taggatcaaa taattgagtt gttgaagcaa tgcttttttc tgtagcaaac ttacctgtca
    38941 agagataatt aaacaaatct ggcattaaaa gaatcttatt ggtc
//
LOCUS       DAVLVZ010000017        36684 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000017 DAVLVZ010000000
VERSION     DAVLVZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36684
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.017"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(99..449)
                     /gene="rbfA"
                     /locus_tag="VYN77_000778"
     CDS             complement(99..449)
                     /gene="rbfA"
                     /locus_tag="VYN77_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1558260.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(700..3492)
                     /gene="infB"
                     /locus_tag="VYN77_000779"
     CDS             complement(700..3492)
                     /gene="infB"
                     /locus_tag="VYN77_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1558261.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKTEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(3509..3808)
                     /locus_tag="VYN77_000780"
     CDS             complement(3509..3808)
                     /locus_tag="VYN77_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1558262.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(3801..4094)
                     /locus_tag="VYN77_000781"
     CDS             complement(3801..4094)
                     /locus_tag="VYN77_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1558263.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(4116..5252)
                     /gene="nusA"
                     /locus_tag="VYN77_000782"
     CDS             complement(4116..5252)
                     /gene="nusA"
                     /locus_tag="VYN77_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1558264.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(5296..5775)
                     /gene="rimP"
                     /locus_tag="VYN77_000783"
     CDS             complement(5296..5775)
                     /gene="rimP"
                     /locus_tag="VYN77_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1558265.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(5901..6536)
                     /gene="trmB"
                     /locus_tag="VYN77_000784"
     CDS             complement(5901..6536)
                     /gene="trmB"
                     /locus_tag="VYN77_000784"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1558266.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(6533..7327)
                     /locus_tag="VYN77_000785"
     CDS             complement(6533..7327)
                     /locus_tag="VYN77_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1558267.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(7488..8099)
                     /locus_tag="VYN77_000786"
     CDS             complement(7488..8099)
                     /locus_tag="VYN77_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1558268.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            complement(8129..8377)
                     /locus_tag="VYN77_000787"
     CDS             complement(8129..8377)
                     /locus_tag="VYN77_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558269.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKNDV"
     gene            complement(8419..9108)
                     /locus_tag="VYN77_000788"
     CDS             complement(8419..9108)
                     /locus_tag="VYN77_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1558270.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFSGVMIWILVKTGHGYLFRIPRKKWLWYSILTVLVLVLQISFNVQIAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     FLPSILFALPHFSSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            complement(9160..9543)
                     /locus_tag="VYN77_000789"
     CDS             complement(9160..9543)
                     /locus_tag="VYN77_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1558271.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            complement(10172..10546)
                     /locus_tag="VYN77_000790"
     CDS             complement(10172..10546)
                     /locus_tag="VYN77_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1558272.1"
                     /translation="MFLARLFLGQLPLLVSTYLFLSRQFLNFSLVFQFLLVVINLTSI
                     LVTVYLTREMRIRKFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAAESYQQIIAY
                     IGAVLCLLIMLLLIWGLKYYKK"
     gene            complement(10572..10736)
                     /locus_tag="VYN77_000791"
     CDS             complement(10572..10736)
                     /locus_tag="VYN77_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558273.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKAEKK"
     gene            complement(11034..11153)
                     /locus_tag="VYN77_000792"
     CDS             complement(11034..11153)
                     /locus_tag="VYN77_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1558274.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(11257..11406)
                     /gene="blpO"
                     /locus_tag="VYN77_000793"
     CDS             complement(11257..11406)
                     /gene="blpO"
                     /locus_tag="VYN77_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1558275.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(11650..11853)
                     /gene="blpN"
                     /locus_tag="VYN77_000794"
     CDS             complement(11650..11853)
                     /gene="blpN"
                     /locus_tag="VYN77_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV1558276.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(11869..12123)
                     /gene="blpM"
                     /locus_tag="VYN77_000795"
     CDS             complement(11869..12123)
                     /gene="blpM"
                     /locus_tag="VYN77_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV1558277.1"
                     /translation="MNTKMMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVALWAGVTGATGGF"
     gene            12415..12993
                     /locus_tag="VYN77_000796"
     CDS             12415..12993
                     /locus_tag="VYN77_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1558278.1"
                     /translation="MKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKTNKE
                     GTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQTKK
                     DYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPSSDF
                     QTKISHCLHCSLKLIGHYYQYRWFLLSTRNLG"
     gene            12920..14563
                     /locus_tag="VYN77_000797"
     CDS             12920..14563
                     /locus_tag="VYN77_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1558279.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            14574..15935
                     /locus_tag="VYN77_000798"
     CDS             14574..15935
                     /locus_tag="VYN77_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1558280.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFINFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            15992..16120
                     /gene="blpC"
                     /locus_tag="VYN77_000799"
     CDS             15992..16120
                     /gene="blpC"
                     /locus_tag="VYN77_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1558281.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(16191..17531)
                     /locus_tag="VYN77_000800"
     CDS             complement(16191..17531)
                     /locus_tag="VYN77_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1558282.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLHTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(17545..18282)
                     /locus_tag="VYN77_000801"
     CDS             complement(17545..18282)
                     /locus_tag="VYN77_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1558283.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITLNSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRPHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(18287..18625)
                     /locus_tag="VYN77_000802"
     CDS             complement(18287..18625)
                     /locus_tag="VYN77_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1558284.1"
                     /translation="MKHMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEKASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKVKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            18901..19230
                     /locus_tag="VYN77_000803"
     CDS             18901..19230
                     /locus_tag="VYN77_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1558285.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKTETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(19398..20447)
                     /locus_tag="VYN77_000804"
     CDS             complement(19398..20447)
                     /locus_tag="VYN77_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1558286.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQDFPENHWPILLFVGITSVLLFLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLIYVLLLGVGKYFLFRQKASKFFTETG
                     LNWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSILAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQAVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(20444..21175)
                     /locus_tag="VYN77_000805"
     CDS             complement(20444..21175)
                     /locus_tag="VYN77_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558287.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLTPYSGSININGLTLQEDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLDQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAIADLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFNMPEASLNDIYLALTKEEEL"
     gene            21243..21653
                     /locus_tag="VYN77_000806"
     CDS             21243..21653
                     /locus_tag="VYN77_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1558288.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTPGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            21663..21950
                     /locus_tag="VYN77_000807"
     CDS             21663..21950
                     /locus_tag="VYN77_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558289.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(22330..23466)
                     /gene="dnaJ"
                     /locus_tag="VYN77_000808"
     CDS             complement(22330..23466)
                     /gene="dnaJ"
                     /locus_tag="VYN77_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1558290.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(23739..24095)
                     /locus_tag="VYN77_000809"
     CDS             complement(23739..24095)
                     /locus_tag="VYN77_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEV1558291.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKGFIDNIPVECLTRY
                     REIILAEHNSCIKNDEAVRNFVTSVLLSAFVSAIVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(24097..25920)
                     /gene="dnaK"
                     /locus_tag="VYN77_000810"
     CDS             complement(24097..25920)
                     /gene="dnaK"
                     /locus_tag="VYN77_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1558292.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVKRIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(26389..26913)
                     /gene="grpE"
                     /locus_tag="VYN77_000811"
     CDS             complement(26389..26913)
                     /gene="grpE"
                     /locus_tag="VYN77_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1558293.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(26940..27974)
                     /gene="hrcA"
                     /locus_tag="VYN77_000812"
     CDS             complement(26940..27974)
                     /gene="hrcA"
                     /locus_tag="VYN77_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1558294.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKTLQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(28138..28647)
                     /locus_tag="VYN77_000813"
     CDS             complement(28138..28647)
                     /locus_tag="VYN77_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1558295.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDR
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLSGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            29254..31587
                     /locus_tag="VYN77_000814"
     CDS             29254..31587
                     /locus_tag="VYN77_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1558296.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATPKETKN
                     VSNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            31600..33063
                     /locus_tag="VYN77_000815"
     CDS             31600..33063
                     /locus_tag="VYN77_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1558297.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNSVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            33063..34631
                     /locus_tag="VYN77_000816"
     CDS             33063..34631
                     /locus_tag="VYN77_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1558298.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIADTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLAEQQRIIEAIESALEKVDEYAESYNRLEQLDKKFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYGNKDETTSYPIYEIPESWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWVSISDMP
                     ISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPATHNEAIISIF
                     PYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPISNHEEMKRIIS
                     KVDLLFQKVSQLFE"
     gene            complement(34579..35184)
                     /locus_tag="VYN77_000817"
     CDS             complement(34579..35184)
                     /locus_tag="VYN77_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1558299.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(35195..35992)
                     /gene="psrA"
                     /locus_tag="VYN77_000818"
     CDS             complement(35195..35992)
                     /gene="psrA"
                     /locus_tag="VYN77_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV1558300.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            complement(36049..>36684)
                     /locus_tag="VYN77_000819"
     CDS             complement(36049..>36684)
                     /locus_tag="VYN77_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1558301.1"
                     /translation="FEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDI
                     TSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDL
                     MWNSTGLGTLGRLAIYHENKNPYAWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQS
                     VIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
BASE COUNT        11492 a         7576 c         6541 g        11075 t
ORIGIN      
        1 gcgtactatt ctagtttcaa tatattttaa aagaatattt ttccatttca aattcaacct
       61 attttcctcc accaaaaaag aggggcaacc cctcttcttt agttcttatc cagattgcgt
      121 agcatctcgt caatcttgtt tccatactcg atggactcgt ctttgacgaa ggtcaaatct
      181 gggattttgt acaatttcaa attgcgacca agttcacgtt tgatggtacc agttgctttt
      241 tcaagcccga tttgggcttt ttggttatcc gaagcaaggt tactcaaaat ggtgtaataa
      301 accttggcaa cagacaagtc acccagcatc tgaacatctg tgatggtcac accttggaca
      361 cgtggatcac ggactttctt ttgcaaaatc tcattgactt cacgcttgat ttccatgccc
      421 acacgatctg tacggaaatg atttgccatg atattccttt ctctactttg aaccaaaagc
      481 taggccagca gacctagcta ttttaaactt cttgattttc agtttaaatt gaaacgaagt
      541 tcaatttgaa gtcatctgct tctttcgccg tggtgaaagt gatggaactg atttatcagt
      601 cccatcacag gggatttttg agaccctagg ctcaaaaata agcaatgaaa ccatcggttt
      661 gcttgcgtcc ctcaccacct aagaaaggag caaaaaatct tatctcttga tttcttccat
      721 gacatacgcc tcaatcacat catccatctt aatatcattg tagccgtcga tcatcaatcc
      781 accttcacgg ccgtttgtaa cttccttaac gtcgtcttta tagtgtttca agcttgcgag
      841 ttcaccatca tagataacga caccatcacg gataacacgg actttagagt cacgggcaac
      901 cttaccgttg ataaccataa atccaccgat agttcccact ttagacacct tgaaggtttc
      961 acggataacc gcttcaccaa taactttttc ttcaaattct ggatcaagca tccctttcat
     1021 agcttcttcc atctcttcga taaccttgta gataatgctg tgaagacgga tttccacatc
     1081 gtcagcttct gcttgttgac gagcttgtgg tgtagggcgt acgttgaaac caacgataaa
     1141 ggcatttgaa gcttcggcaa gggtcacatc tgattcgttg atagcaccga ccgctgagtg
     1201 gacgatagtc actttgacac cttccacgtc aatcttttga agtgaggcag aaagggcttc
     1261 aacagaacct tgtacatcgg ccttgatgat aacattaaca gatttgagtt ccccagcttt
     1321 aagggtatca aagaggtttt caaggctaac acgttgggta gcttgacgtt gtttcattag
     1381 ggcacgtttg gcacgctctt cacctgctgc acgcgcagat ttttcatcct cgtaaacggc
     1441 aaagtggtca cccgccatcg gtgcttcgtt caaacctgtg atagagactg gtgttgatgg
     1501 tccagcaact ttaacacgac gaccaaggtc gttggtcata gcacggacac gaccgaaggt
     1561 atttccgaca acgattgggt cttgaacatt caaggtacct tgttgtacaa gaagggttgc
     1621 gaccgcacct tttcctttat ccaagcgcgc ttcgataacc gtaccgattg cacgcactgt
     1681 tgggtctgcc ttgagttctt ggatttcagc tacaagaagg actgtttcca acaattcttc
     1741 gatattttgg ttgaatttag ctgagatttc aacaaattca gaatctccac cccaagcagt
     1801 tgacataact ccatgctctg ccaattcacc gataacgcgt tctgggttag cacctggttt
     1861 atcaatcttg ttaatggcta cgatgattgg aacgttggcc gcttttgagt ggttaatggc
     1921 ttcgatagtc tgaggcataa ccccgtcatc tgccgctacg accaagatcg taatatcggt
     1981 aacagaagca ccacgcgcac gcattgatgt aaaggccgcg tgtcctggtg tatcaaggaa
     2041 ggtaatcttc ttaccatttt ccacgatttg gtaggcaccg atatgctgag taataccacc
     2101 tgcttcacct gtcgcaacac gtgagttacg aagagtatcc aaaagggttg ttttaccgtg
     2161 gtcaacgtgt cccatgatag taacaactgg tggacgctca accaattcat cttcattgag
     2221 ataaccatct tcgacaaaga aacgttcgat gtcagcatta tccacttcaa ccttttgttt
     2281 ggcttcgata ccgtaatcca ccatgaggag ttcaattgtt tccccatcca aggattggtt
     2341 ttgtgtggcc atgacaccca tcatgaaaag tttcttaaca atttcagctg gttcacgttt
     2401 gatacgtttt gcgatttccg caacggtcat accatctgta tattcaaatt ctgttggcaa
     2461 ttcatggaat ttacgctccg taacaggttt tggagtctga ttacggttgt tcttgttatt
     2521 gccttttttg ttctttttgt tgttattcca gttactattc ttttgatttc tcacttgatt
     2581 ttgactactt cgattctttt gttgttttct aggaccatct tcttcatgat cataatcgtc
     2641 acgatttttg tctggtcgag cttgtttttt acgacgtgta tccactgcag gttctttttt
     2701 ctcagggacg acttcaacca ctgcttgaac ttgctgtgct tgttcagcta acttagccgc
     2761 ttcttcaaag atttcctctg gttccttgcg tttgttagct tgagccaagg cttctttagc
     2821 agcctgatac tgcttgaagc gttcctcact tgaacgagcg tactctgcat tttgctctgc
     2881 ttttagggct gctgcacggg ctttaaagtc aatacgtgga gccgcttgat ttgaacgttt
     2941 gtcctcttgc tgacggcgct catttctacg tttttgctga ccttgctgct tcttagcttg
     3001 gtcattaaag cgacgattgt cgcggttgct ttgaccttgt tttccaccat tacggccatc
     3061 gtttttctga cggtttccgt tttgttgttg gtcacggtta ttgcccttat tttgcttgcg
     3121 tcgttctgcc tgctctttag cacgggcttc acgctcagcc ttgaaattac ggctttgcgg
     3181 tttcgctgct actttttctg ttttcggagc aactggctca gccggttttg cctcttcctt
     3241 agctacagct ggtttagctg gctcagattt ttcggttttc ttttctgcac ttacttttgg
     3301 tgctgcaggt tttgcttcta ctttcggagc agctgcaggc ttaaagctgg cagcaatttt
     3361 tgcagcgaca gcttcttcca cacttgatga gtggcttttc acatccaagc ccaactcttt
     3421 tgcacgcgct acaacttctt tactttcttt tccaagttct tttgcgattt cgtacaatct
     3481 tttcttagac aaatcatgtc ctcctcttct attccataag agacctcatt ttctttgtaa
     3541 atccagcatc tgttacagcc aaaacctttc tcgatttccc gactgctatg cttaattcca
     3601 gtgttgaaaa cacggttaca atttctactt gataataatg acttttatct tgaatcttct
     3661 tggtcagatt gggtccagca tcatgagcta gaaagaccaa cttggccttg ccgtcttgaa
     3721 tggccttgac caccaattct tcacccgata tgatgcgccc tgctcgctga gcaagcccca
     3781 agagattact tatcttttgc ttattcaagt cccaactctc ttcttttcac tttgtgatcc
     3841 acataagcga tcaactcgtc ataaaagctt tcttccactt ccatgctaaa gctgcggtta
     3901 aagaccttct tctttttcgc ctctagggct tctgcattgt ctagtttgat ataagcgccg
     3961 cggccattgg ccttgcccgt aggatcaata aagacttgtc cttccttgtt cttgacaatg
     4021 cggagcaaat cacgcttatc aatcacttcg ttagacacaa cagacttgcg caaagggatt
     4081 tttcttgttt tcatctttcc ctcctctagc agcttttatt cttctacagt atcgttttct
     4141 acttccaact ctactgaagc agcgtcttcc atggcttcaa attcgctagc agacttgata
     4201 tcgatacggt aaccagtcaa gtgagccgcc aagcgcacgt tttgtccacg acgaccaatg
     4261 gcaagagaaa gcttgttatc tggaacaacc accaaggcac gtttgctgtc gttttcatca
     4321 aagataactt ggtcaacctc agcaggagcg atggcattgt agataaattc agctggatct
     4381 gctacccact cgataacatc gatattttct tcgattggta ccatgcggtc atttttagca
     4441 tcgtaacgag ctgggtggaa tttgctagta atcttcttga tattagcacc accacgtcca
     4501 acgattgtac cgatagcatc cacgtttgga ttgtggctac gaacagcaac cttcgtacgg
     4561 tcacctgctt cacgagccac gctcatgatt tcaacagttc catcataaac ttctggaatt
     4621 tcttgctcca tcaaacgttt gatcatttct ggatggctac ggctaacaaa gacgttcaca
     4681 ccacgagggt tgtcttcaac cttgtaaaca taaacttcga tacgatcatg agaagcaaaa
     4741 acttctccag gaatttggtc ttgttttgac aattgggctt cgatgctacc aaggttgaca
     4801 tagataaagc ggttgtcaaa gcgttctact gtaccagaca tgatttcttg ctcatgttct
     4861 ttgtaagtat tgtaagtgat ggcacgtgtt tgcttgcgca ttttttccat gatggtttgt
     4921 ttggcagatt gggctgctac acgaccaaac tcagctggtg cttcttcaaa cttgattttg
     4981 tctccaagtt cataagctga attaatggca agagcatctt tcaagctgat ttccaaacgg
     5041 ctatcaaata cttcatcaac aacttcacgg acagtataaa ctgtaaagtc acctgttttt
     5101 tcgttgaagt caatagctac gctgtctgac tgaccatagc gtctgcgata agcggaacga
     5161 agcgactcta ctactgcgtc gatgatatct tcttttttga ttcccttgtc ttcttccaaa
     5221 atgcggaagg cctctagcat ttctttactc atgttttctt cacttttgaa tcctcaaaag
     5281 ctatcctttc tttttctata atttaactgc taaacgtgct tttgatacta aactgtatgg
     5341 aatttggacg gttttcttac gcgtcttgtc catatattcc atagtcaact cgtcctcttc
     5401 gaaggccaac aaggttcctt caaagacctt ttgcttatcg atggcttggt agagcccgac
     5461 atggatgtat tttccaaccg ctccagcgac ggcatccttg gttttcaaag gacgttccaa
     5521 gcctggactg gtaatttcta ggaaatattg ttctgggaag ggatctggct tgatggtgtc
     5581 taggacagga ctgatcattt ctgttaagtc tgccgtgtcg ttcaaggtaa ttccttcggg
     5641 tttatctaca aaaatactga gaatcatgtc actgccaatc tttccatact cgatatccac
     5701 gagttcaaaa ggagcttcta tgacaggttc tacaacttct ctgactaatt ctacgattgt
     5761 tgcgattgcg tccacctcct cataagcaag aggcgaagat atttccccgc ctcacttttc
     5821 atattcttac tatcagtata gcacgtttgc caacttatgt aaagcaaaag cacttgtaca
     5881 gcctaatttt aggctatctc ttaaaattct gcctcaactc ggtagataac ttgccccttg
     5941 tttgagaatt tttgctcgta ttctgtcatg acattgcctt caaaatcact ggcatgcaaa
     6001 tctagccaga caccattgag tttcatgcca tattgagaaa agctcactaa actgtactca
     6061 aacaagccac ggttatccgt cttgaaatga atttctccat tttcaggcaa gatacgtttg
     6121 aaggtgtcca aaaaggtctt gtaggtcaaa cgacgctttt catggcgttt tttcggccat
     6181 ggatctgaaa agttcagata caagcgatca atctcaccgt cttcaaagta gtcagtcaag
     6241 tcagaaccat ctacccacaa gagcttgatg ttaggcactc caacttcaag caccttgtcc
     6301 aaagcgtagc tcaaaacaga cttttgaata tcaatcccga tatagttgat gtcagggttt
     6361 tgcttggcca tacctgaaac aaaggcaccc tttccacttc caacttccac atgaatggga
     6421 ttatcattgc caaacaagtc ccgccatttt gccttggctt ccaagggatt gaggaccaca
     6481 tactggggat ttgcctctag taattctgtt gcccctttac gatttctaac tctcatcttc
     6541 tctttccata cttgtctcgg aaatgacgca aaccatgaat ctcccgattg acattttcta
     6601 aatcttggtt catataatac ttggaaatct gactcaaata agacaattga ccataccaat
     6661 acaatttact taataccgtt tgattgtact tgtaaccgta gtaggtcaac cattccttcc
     6721 actgatgttc tgaaatataa tggcagagca tatgggccac atcaaacatg cgatcggtca
     6781 agcgaaccga atcccaatct actaaataaa tcaagccact atctgtctca atccaattac
     6841 tatgtcgtac atctccatgg acaatggtcg catggtcctc tctaaatcct ggaatagtct
     6901 gacgtaaatc agccatcact tcactgataa aatgattttt acgcaaagca tctggagccg
     6961 tttcttgcca agactgtagt aaatctacag gtgtttccat ggcatagccc aaacgactca
     7021 attgtgtcat caacggacgt gagcgatgaa gacgggttaa aatattgacg atttgcttac
     7081 gattcatatc atagggggtc aatatcttgc ctgtcaacca ttcttgagca cacatatcac
     7141 gcccatctgc caaacgacga ctccataata attgtggagc aatttgttct ctagctagac
     7201 caggtaggat tggagaggtg ttcattttta caaagatgcg cttcccatca ggatagctac
     7261 ccatataagc cttgccactt ttcccaggta tgggagtcag tgttagctca ttatcaccca
     7321 aatccatttc tttcctccgt ataaagtttc cttactattc tactagtttt tggcctattc
     7381 gtcaaccact ctaacccgat tctcaaagaa aagcctctgg agtggtttgg gtatcattat
     7441 agcttgaaaa aggtaagcac cgtcttctgc ttaccttttc ataattttta taaataatat
     7501 aggagtggcc aactgttgta aaacatgtga actagagtag aaacccataa gttttggctc
     7561 tttttataag caaggcccag caacaagccc ccaagtagat aaaaaataaa cgaagggaca
     7621 ttagaaggtc catgcatgaa agaaaagaga gaggaagtta gcacaagccc taaccaagaa
     7681 ttgtcaaaaa ccgcaccttg aagaagtcct ctgaaaatga gttcctcctg gatggggcca
     7741 aaaactgagg tagtcaaaat caaagacagg gaaattcctc tgctgacttc cgccaagtgt
     7801 tggacccctg agccagaaga aacagcgaag aaatgaatat aaaccagcgt ctcaagcaca
     7861 cttagaagaa agattgcaat gaaaagcaaa agttgtttct tgcttaacat cccaaaagaa
     7921 atcatttcaa actttctagc ataagcaaca ctcagcgagg ttacaagaag acttacaata
     7981 atcaatgcag attcattttg aaaaaaatcc ccatggttaa tcgtataagg aactgtaatg
     8041 acaattatta gtactaaaaa tccaaaacac aaggcatgaa atttatcaaa aaactccata
     8101 aaattatcct cctcaacaaa cagacttcct acacgtcatt ctttagttct agcctttcca
     8161 aaacaaacca ttttagcaac caaaatccga ccacatagcc agcccctatg aatatagcca
     8221 ttaaagctag catggaattt aggaaattaa aaaccaccgc agatacaaag gttagcacaa
     8281 aaacattaaa agcaatggtg tcagaagcca agactagaat atagggtgtc aaccgatcta
     8341 aagttttgga atctaggaaa aataagtgtt tatacatgat gacctcctct atggctgaaa
     8401 agcaagcctt ttgttttttt accccaagac cctatgtaga aaagtgagca aaaacgggaa
     8461 ggtcgctaca atattattga tcacatgcac cgcataggat ggataaatac tcttggtata
     8521 gcgggtcaaa ccggcaaaga tgattccaaa tgttgcaaag acgaaaatat ctaacaaact
     8581 aggtaggctt gaaaaatgag gaagagcaaa taaaatggac ggaagaaaca aatcaaggcc
     8641 aaatcgcgaa tgcttaaaga aggcgtgttg cagtaatccc ctataaatca actcttccat
     8701 tagtggagtc aagaaaaaca gggttacata gatacctagc tcagcaaagt tggtcccact
     8761 ataaccgatc aatacggccc aaccttcagc agttgactga acatgtttag ctatctgaac
     8821 gttaaaagag atctggagca ctagcactaa tactgtcaaa atcgaatacc aaagccattt
     8881 ttttcttgga atgcgaaaga gataaccatg gcctgtctta accagaatcc aaatcatgac
     8941 tccgctaaat agtaaactca agatattttg aatccagacg aaattaccaa tctgggaaga
     9001 aaattgccaa taattttgga cgataagcgt cagctgagaa agaccaaata cgaaaaataa
     9061 gtaagagaag actgcactta ttttgaatag aagttgatac tttttcatag aaatcctccc
     9121 tactatgacc tcaccttgtc aggctctact gctgtaagat taagaagaca gtttgttttt
     9181 tttaaggcta acctgactac tagataatag atacattaag gcattaaaga caatgaaaat
     9241 atgtccatag aataaaatca acctcgcatc caaaccaaga taaagtttga ttatcaaaaa
     9301 gatgagcaaa agaatttgaa accataaggt ttttccaaaa ataaatttaa agcgatttcg
     9361 aatatctact tccttgattt ttaccgccac ccctttatta gcaagaagga aaactcctgc
     9421 ttcaaacaaa ccactgtaaa gaacaagcca cccaatagat acgatagaga tttgtaaaaa
     9481 tgtccctaaa agaatatcca acacactact caagaaaata acaaaaaata atctgtattt
     9541 catattaaat acctccattc atttatttca ctaacaattt aatagagcct tctactcaaa
     9601 tatcctgtca gaaaaggata gaaagctact ttttataata cttcaagccc cacatgagca
     9661 gaagcgtgat aaacaagcag agaatacacc tatataagcg attagttgtt gatagaattc
     9721 tgtttctgaa atacctctat acaaacaaat gacaaacata aaatctgcca agccgataaa
     9781 cataagttga ttggttctag gactaaccaa atcatcattt acttatattt aagagtatct
     9841 cttttatgtt aatgtatgtt agcactgaaa agcaagacag gccaataata tttaaaatga
     9901 acagtaacgg ggttaagtct ctaaaaaaat tatctactga cactacaaga aatactatac
     9961 atattatagt cgaaactatc tttttcttat ccataattat ttactccttt cctaacaaat
    10021 ccagcttatc aatcaagagc gatttttaac ataatgtagc agcacccgtt gcaactttga
    10081 caagtttagt atatcattgt tttttaaaat ttttcatcca aatcttgaat tgtcatcgaa
    10141 acctcctaaa ttgcaaaaac ttcattagcc cctacttttt ataatacttc aaaccccaaa
    10201 tgagcaaaag catgataagc aagcagagaa ccgcgccaat ataagcgatt atttgttgat
    10261 aggattctgc tgctgtgata cctctataca aacatataat agacataaaa cctgtcaagc
    10321 cgataaacat gagttgattg gttctaggac taaccaaatc atcatcttca aactttctta
    10381 tcctcatttc cctagtgagg taaacagtga ccaaaataga agttaaatta ataaccacta
    10441 aaagaaattg aaaaactaag gaaaaattta aaaactgacg agatagaaat agataagtag
    10501 agacaagcaa gggcaactga cctaagaaca atctcgcaag gaagatgttc cgtttttagc
    10561 aagaaaagtt ttcatttctt ttctgctttc tttttattga tagcaaaata gatcataact
    10621 gcaatcacat aggctatggt ataaaatagc tgataccaag cactctccct aagcggatat
    10681 agaaagatgg acatgattag atacagaacg aaaataatca gtattttttt cttcataaga
    10741 tttcctccta aatgtgcgat ttatcttagt tgagcaagaa catttacact gctagtatag
    10801 cacttatttt gaccttggat cactcaaatc ataaatggtc atcaaaacct cttgaattgt
    10861 aaaaattaaa aaaaccaagc atgaaaaaca tactttcctc tttatattgt attgatacca
    10921 acttgtttgt agacttttca tcctgctatc acatatcatt ttgacaggcg aaacaatatt
    10981 aaagaaactc ccctgtaaat taagctagca aatacagggg agaaatttat tttttagaga
    11041 gtactatccg tatccttttt ggaagatttt gaaaatattt ttctaattaa gtcatccata
    11101 taaggaccaa atataccaac tactaaacca ataataaaac ttttaaaatc cataattacc
    11161 accaacatgt tgctgcatag gctacacctc caagtatagc tccacctgca gcaccagtta
    11221 cacctattcc tatagcaaat ggtcccaata gaaatgtcaa accgttgttg cacacccatc
    11281 aattgcgcca tatgcaaccc ctgctgcaca actaattttt cttccccaat caatatctcc
    11341 accttcaacg caagcaagca tttcattatc cataacagaa aattgtgaca tcatttttgt
    11401 attcatatag tatgtcactt ttcagttaca gaacaagttt aatataaaaa ttatcaaaaa
    11461 aacataagca ataaagagaa aaattaattt atcatagatt agaaataata tgacaaaaca
    11521 attcaatgat gttaattcaa tagtcttttg ttttttatca gagatactta tggatagata
    11581 aataagatag gtttgaaaag cgaagagaat aataaagaat atagccttca taaaatttag
    11641 ctttcatttt tatgatgtag cggtataggc taaatatcca caaaccactg ctcctccaat
    11701 tcctcctatt gcagcgcccc atggtcctag aattttccca tatttcactc cacccgctgc
    11761 acaacctaaa gcagcaacta cagctgctcc tccggaatta cctccgtaaa cctcactcag
    11821 cattgtttca tttatattac aataagtatt catacaagtc tccttttgtt aaaatccacc
    11881 cgttgcccct gttactcctg cccaaagagc cacaccaaat ttagctccta tgtatccaca
    11941 tgctcccata aatggcgctc caacaccact cgcagcacaa atagctgtcc ctaaccccca
    12001 gccaccaaaa gcagcaccac caccttctaa gacattagtt tgccaattat tcttacctcc
    12061 ttcaatacta gataacatag ttatatccat ttcatgaaat tgttccatca tttttgtatt
    12121 catgacaaat actccttttt tcttttttta tttttgtctt gttgtaactt tgataagttt
    12181 agtatatcat cgttttttaa aatttttcat ccagatcttg aattgtcatc gaaacgtctt
    12241 gaattagctt ttttatttca agccacctct aaatgtttaa aaaaaataat ttctaatcac
    12301 ttttttacca ttcaggaagt tttaatgact attcaagatt tcataaaata tgaacttagt
    12361 tttatgacat aatagaccta tccactatat gaaaggaatt gccaatgact tcttattaaa
    12421 cgtacatttg ttcctcaaat agatgcgaga gactgtggtg tcgctgcctt agcctcgatt
    12481 gctaaattct atggttcaga tttttctcta gctcacttga gagaacttgc aaagaccaat
    12541 aaagaaggga cgactgctct tggcattgta aaagccgctg atgaaatggg ctttgaaaca
    12601 agacctgttc aagcagataa aacgctcttt gacatgagtg atgtccccta tccatttatc
    12661 gttcacgtta acaaagaagg aaaactccaa cattactatg ttgtctatca aacaaagaaa
    12721 gactatctga ttattggtga tcctgaccct tctgtaaaaa tcactaaaat gtcaaaagaa
    12781 cgctttttct atgaatggac tggagtagct atttttctag ctaccaaacc cagctatcaa
    12841 ccccataaag ataaaaagaa tggtctacta agcaagcttc cttcctctga ttttcaaaca
    12901 aaaatctctc attgcttaca ttgttctctc aagcttattg gtcactatta tcaatatagg
    12961 tggttcttac tatctacaag gaatcttgga tgaatacatt ccaaatcaga tgaaatcaac
    13021 tttaggaatc atctcagttg gtctggttat cacctatatc ctccaacaag tcatgagctt
    13081 ctccagagat tatctcctaa ccgttctgag tcagagatta agtattgatg tgattttatc
    13141 ctatattcgc catatttttg aacttcccat gtctttcttt gcgacacgtc gtacaggaga
    13201 aatcatttca cgattcacag atgctaactc tattatagat gccttggctt ctaccattct
    13261 ttctcttttt ctggatgttt ctattctgat tcttgtagga ggcgtcttac tggcacaaaa
    13321 ccctaatctc ttccttcttt ctcttatttc cattcctata tacatgttca tcatcttttc
    13381 ttttatgaaa cctttcgaaa aaatgaacca tgatgtcatg caaagtaatt ctatggttag
    13441 ctctgccatt atcgaagata tcaacgggat tgaaactata aagtcgctca cgagtgaaga
    13501 aaatcgctat caaaatatag acagcgaatt tgtagattat ttggaaaaat cctttaagct
    13561 cagtaaatat tctattttac aaacgagttt aaagcaggga acaaaattag ttctgaatat
    13621 ccttatccta tggtttggcg ctcaattagt catgtcaagt aaaatttcta tcggtcagct
    13681 gattaccttt aacacacttt tttcttactt tacaactcct atggaaaata ttatcaacct
    13741 ccaaaccaaa ctccaatctg cgaaggtcgc taataaccgt ttgaacgaag tctatctagt
    13801 cgaatctgaa tttcaagttc aagaaaaccc tgttcattca cattttttga tgggcgatat
    13861 tgaatttgat gacctttctt ataagtatgg ttttggacga gataccttaa cagatattaa
    13921 tctcacgatt aaacaaggag ataaggttag cctagttgga gttagtggtt ctggtaaaac
    13981 aactttagcc aaaatgattg tcaatttctt tgaaccctac aaaggacata tttccatcaa
    14041 tcatcaggat attaaaaaca ttgataaaaa agtcttgcgc cgtcatatta attacctacc
    14101 acaacaagcc tatatcttta atggctctat cttggaaaac ttaactttgg gcggtaatca
    14161 tatgattagc caagaagata ttctaagagc ttgtgaatta gctgaaattc gtcaagacat
    14221 tgaaagaatg cctatgggct atcaaactca gctctctgat ggagctggtc tatcaggagg
    14281 acaaaagcaa cgaatagccc tcgctcgtgc tcttttaact aaatctcctg ttttaatact
    14341 agacgaagct actagcggtc ttgatgtctt gactgagaaa aaggttatag ataatcttat
    14401 gtctctaact gataaaacca ttctctttgt agcccatcgt ctcagtatag ccgaacgaac
    14461 caaccgtgtc attgttcttg accaggggaa aatcattgaa gttggtagtc accaagagtt
    14521 aatgcaggcg caaggtttct accatcatct attcaataaa taaggagaat gtcatgaatc
    14581 ctaatctttt tagaagcgtc gagttttatc agagacgtta ccataactat gcgacagtgt
    14641 taattatacc tctttcatta ctatttactt tcatcttgat tttctccctt gttgccacaa
    14701 aagaaattac tgttacttcc caaggagaaa tcgcccctac aagtgtcatt gcctccattc
    14761 agtcaaccag tgataatcct atcctagcta atcatttagt ggcaaatcaa gtagttgaaa
    14821 aaggggactt actcatcaaa tactctgaaa caatggaaga aagtcagaaa actgccttag
    14881 caactcaatt acaaagactt gagaagcaaa aagaaggact tggaattttg aaacaaagct
    14941 tagaaaaagc gactgatctt ttttctggcg aggatgaatt tggctaccat aataccttta
    15001 taaattttac taaacaatcc catgatattg aactgggtat cacaaagact aacaccgaag
    15061 tttcaaatca agctaatctt tccaatagca gttcatcagc tattgaacaa gaaattacaa
    15121 aagttcaaca acaaattgga gaatatcaag agttgagaga tgctatcata aataacagag
    15181 cacgcttacc aactggcaat ccgcaccagt caattttgaa tcgttatctt gtagcctcac
    15241 aaggacaaac acaaggaact gcagaggagc catttttatc tcaaattaat caaagtattg
    15301 caggtcttga atcatctatc gcaagcctca aaattcagca agctggtatc ggaagtgtag
    15361 caacttatga taacagttta gcaaccaaaa ttgaagtact ccgcactcag tttttacaga
    15421 cagcctcaca gcaacaacta actgtggaga atcaattaac agaattaaaa gtacaactag
    15481 atcaagcgac acagcgctta gaaaataata ccttaaccgc tccaagtaaa ggtatcgttc
    15541 atctgaacag cgaatttgaa ggtaaaaata gaattccaac tggtacagaa attgctcaaa
    15601 tattccctgt catcacagat acaagagaag tactaatcac ttactacgta tcatctgact
    15661 atctcccctt actagataaa ggacaaactg taagattaaa actggagaag attggaaatc
    15721 acggcaccac catcatcggc caacttcaga gtattgatca aactcctacc agaacagagc
    15781 aaggcaatct ctttaaatta accgctcttg caaaactatc taacgaggat agtaaactca
    15841 tccaatatgg cttacaaggt cgcgtcacta gtgtaactac aaagaaaaca tattttgatt
    15901 atttcaaaga taaaatttta acacattctg attaattttc agataacact ctataactat
    15961 ttattatctt atcaaaaagg agaatcataa catggataag aaacaaaacc taacttcatt
    16021 tcaagaacta acaactaccg aactcaacca aattacaggt ggaggattgt gggaagattt
    16081 attatataac attaatagat atgctcatta catcacataa gaacttcatc atccaataca
    16141 actataaaaa aataagaccg agaaacaagt actctcggtc ttatttttca tcattctgta
    16201 tgtatcacag taagtacctg acgaaagact tgattttgac aggtggtatt tagactggta
    16261 ttaggatggc tttccacaat cttcatgacg gtatggagac caactcctct ctcctcccct
    16321 ttagaactgg ctccaaagga gaagatttca gaaatatcga tgccctcttc tttgatggag
    16381 ttttcgatga taaaggtctc ctgtgctcca ttttttaaaa aggcgattga aacatgaggt
    16441 tgactagctt ccacactggc ttcaatagca ttgtcacaaa ggatagacac aatggttaga
    16501 aaatcaagta gactcatccc ctcgacctga atctcctcag gaacttcgac attaaagaca
    16561 atgttcttat ctctggcttt taaaaatttc cctgctagaa gacttttgag ggctttatca
    16621 cgaatattta ccaatctgcc caggtcatat ttattgttct gcaatttctg actggaatcc
    16681 tttaagacgg agccatagac ctcttttatc tgctccatat cctcctcttc aatgcccaga
    16741 cgtaagctag tcaagaggtt ggtataatca tgacgaaagc tccgtacttc cttgtaaagc
    16801 tcctctatat gccgactata gcgttccata tctctatagc gcagggcctg ctcttgttcc
    16861 aatctctcat agagtttttc cttcaaatag gtatccaatt tcttgataac ccccataaaa
    16921 aagagtaggt aaaagactag gatgagatgg cgaacagtct ttgattgaat actttgttca
    16981 tattcaaaaa aagacagact ttccatgact agatagtagc cacccattat ccagttaatc
    17041 tgagtcaggg acttttgaaa ggctttatcg agaatctcct ttctcaagct agtaaaatcg
    17101 tagtccaacc atttcaaaaa agctagagaa atgaagaaat tgaaaattat tatacataac
    17161 ccagtaaatg agtagccatc atatacttgc ccttgtccca aaaatggaag cacaaaatag
    17221 gagactcctc tataaaagag attcaccaat atcattggaa agagaccata aaagaaaagg
    17281 agttttttag gaagccctct caataataag aaagataagc ctatgccgta caagggttcc
    17341 ataaaataag ataggtaaac atttcctact atatagctaa tcatcacaaa aacaaaggcc
    17401 aacagtatct taaaaagaaa ggccttaaaa atcctctcga aagtaagatc aattccatcc
    17461 accttaaaga agatgacaat ttctagtcca ttagtaacaa gtgtatacaa caatatccaa
    17521 gcaatgttca taaattctcc tagctcagtg taatttattg atggcctcag acacttccct
    17581 gaccttataa cgcgcgatca gacaacttcc accattggga aagaaaagga gtttttcttt
    17641 cttatccaaa tgcaccacat ttgcaggatt gatgagaaaa gagcgatggc actgcaagag
    17701 acggggttcc tgcttgaaaa cctcctctaa actcgctgta aattccagcc tgtctgtctt
    17761 ggtatagaga ataacacgat ggggtcttgg ggatgtttcg agatagtaaa cctctttaaa
    17821 aggatactga aattgggcaa attttgattt aaagtaaaag caatcttccg ccagactttt
    17881 actatcttga ctattggcat agaggagagc tgtctcgatt cgagattcaa actcctctgc
    17941 cgaaagggcc ttatcaatat agtccagagc agacacttgg tagcgaaagg acaggggcat
    18001 aaactccgag tgagtcgtca caaagacaat cagggcatac ggatcccgat cccgaatctt
    18061 tctagccact tccagaccct tcatctcctc atttcgaatc tcaatatcca aaaagaatag
    18121 ctgatgggcc cccttctcat gcacctctgc cagcaattga tctggcttac caaaaacttc
    18181 aaaagaattc agagtgatat gatgttcctt caagagtttc tcaatcgtcg tttcaattct
    18241 agtctgttgg gaaaaatcat cttctaaaac aaatattctc atcttcttac tctccttctt
    18301 tcaaccattt ttgacgaatt tctctatagc gacgtctggc atacttgaca gcgtattgac
    18361 cttcttctcc aagaaaaagg attctttctt gaaaatcaat cgattttact ttatcaagat
    18421 tgaccaaaca atttcgatga catctcatca aggtttcccc atattggttc tcaagattac
    18481 ttaaattttg aagcatatta aaactagctt tttctgtaac aatctgtacg gtatgggctt
    18541 tagttggatg tgtttggata tagtagatat cgtcgatgtt tactttatag actgttttct
    18601 gcgtctgaat aatcatatgt ttcattggat tctcctccta agaaaagaat aacagaaaag
    18661 ttctagccca agcccaccca atcctaaaca gtctttgaaa actcctaaaa ggattatttt
    18721 ctacaacatc atgatgagct tacaactatt ttatcataaa attcactcag cgcaattcaa
    18781 gacatttcaa tgacaattaa agatttgaat aaaaaaaaag aattaaaagt gataaactaa
    18841 cagtatcaaa acacagttgc cagaagtaag actggcaccc agattaaagg aggcattccc
    18901 atgacagata cagaccccat caaaagagct catactttga ttactgactt aaacaaagcc
    18961 taccaagcat gcaaacaggc aagcgctgac gacgtccgct ttcaggagca attaaactct
    19021 attcttggtt ttctagccaa gactgaaaca gtggataatc gattcttgat tgaactagaa
    19081 aaattttacc agacttccag tcttctcatg ggactcagcg ctcttgatcc agatgctcca
    19141 actcgcgccg cttggcgggc ctatgaccgc ttccactttg accaagtcaa gaccaagtta
    19201 atactcaatg agaatcaaag agcaaactag gaagctagcc gcaagctgta cttgagtacg
    19261 gtaaggcgac gctgacgtgg tttgaagaga ttttcgaaga gtataagtct ctacggacca
    19321 accattatcc tgtagtaaca aaaaagaagc tgagacaaac tgaactcaac ttctttttta
    19381 gtgtcatata ggcaagatta atcctgcatc tgacgtttta cctgataagg caaatacaag
    19441 actagtaaaa ccaaaccagc tcccagtaag gctagaaggg ctagtttttc ttggaaggta
    19501 atcaacccaa caactaattc caccagtaaa acaaaactgg tcaatcctcg gactacagcc
    19561 tgtaagcctt tttccttttg ccccttgtcc agtggaaaga gttgggtcaa atactggtag
    19621 tcaaaggcat gatagagggc cagcaactga aagagcaaga ggtagttaaa gagaaccacc
    19681 actgctgtcg caatccaagc ttgctcgata aatacctgcg ccagtatgga aagcaagagc
    19741 agacggagac tgagcgcaaa gaggtctcca tttcgcagat aagaacgtag atagagattt
    19801 tgccaaatct ttccaggcac cttctgaaca gcctttagga taaagtccag ataggcacga
    19861 cgcttgacgc tgtttgaaat tcccttgacc tgcgtaaaga gggcaaagaa acgaagcaag
    19921 acttgcttgc gcttgctttc ttgagaaata acatagtccc agttcagtcc agtttccgtg
    19981 aaaaatttgc tggccttttg acgaaagagg aaatattttc ctactcccaa taaaagcaca
    20041 tagattagga agacaggcaa accataaccc atggctaaaa ataagggcgc aaataacagc
    20101 aagaaaaggg tctggacaaa gagccaaaag actagggaaa tgccagtttg acgcttgaga
    20161 tggagtttga tttcctcttc tccgactaag agaaagagct tgtctggagc ctccatatag
    20221 gtggcaattc caccccaaag aaaaagtaaa acagaggtaa ttcctacaaa taaaaggata
    20281 ggccaatgat tttcaggaaa atcttgcaag agttgactgt actggtaggc tagaaagccc
    20341 agcaagacaa gcaggaacaa gacaaagtgg tcattgagaa catagcgcag ataaccaaga
    20401 cactccttac gaaaagcctg ctttctcttt aaaaacaagt ctttcatagc tcctcctctt
    20461 tggtcagagc caagtaaata tcattcaaac tagcctcagg catgttaaag gcttcgcgca
    20521 gttgcaggag atttcccttg gcacgcacct ctcccttatg aagaataaca aaggcatcac
    20581 acatcttctc cgccgaatcc agcacgtggg tactcataag aatggacttg cccttttgct
    20641 tttccacttc caaaagctga atcaagtctg caatcgccag cggatccaga ccaaggaaag
    20701 gctcatccac gatgaaaaga ctcggatcta ccacaaaagc acagataatc atgaccttct
    20761 gcttcatccc ttttgaaaaa tgaacaggaa accagtctaa tttctggtcc agacggaaca
    20821 tttttaacaa gggctctact cgctcaaaag ccactttctg ctcaatacca taagccatgg
    20881 caaccgtttc gatatgctct ctgagggtca attcctcata cagactaggt gtctcaggaa
    20941 tgtagccaat ttgcttgcgg taactagtcg catcttcttg cagggtcagg ccattgatat
    21001 tgatggaacc actataaggt gtcaacagac cgataatctc attaatcgtc gtcgatttcc
    21061 cagcaccatt gagaccaatc aaaccaacca actgcccact ttcaacagta aaagacacat
    21121 ctttcaagac agggacatga acatagccac ctgtcaggtt tttaatttct aacatatttt
    21181 ctccaaatct ggtataatgt agctatatta tatcaaaatt caatacagta gaggtagatt
    21241 ttatgtcaga ttgcattttt tgtaaaatca tcgcagggga aattcctgct tcaaaagtat
    21301 atgaagatga gcaggttctt gcctttcttg atatctctca agtgacacca ggacacacct
    21361 tggtcgtgcc caaagaacac tatcgcaatc ttttggagat ggatgctacg agcgccagcc
    21421 aactctttgc ccaagtacca aaagtagctc aaaaagtcat gaaagtcact aaggctgctg
    21481 gtatgaatat catttccaac tgtgaagaag tcgctggtca aacagttttt catactcacg
    21541 ttcaccttgt gcctcgctac agtgctgacg atgacctcaa gattgatttt atcgcccacg
    21601 aaccagactt tgacaaactt gctcaagtcg ctgaaaccat caaaaacgct taaggagttg
    21661 ctatgaaatt atccaaccta ctgctatttg caggagctgc agccggaagt tatctggtta
    21721 caaaaaatcg ccaaaccatc acagatgaag tcttgaatac cactgaccgc gttcaagcta
    21781 tcaaggacga tgtggatatt atccaaaata gcctgcaaat catcgaccag caaaaagaac
    21841 ttatcaagga ataccaagaa gacttgactt acaagtttaa ggtcttggaa aaggatatcc
    21901 agactagact agctgtgata aaagaaatgc agggaactga agataagtaa aaagagcccc
    21961 ccggctcttt tttaaaacgt aaacaagtta tagtgaattg aatttagaat agcacaccat
    22021 agtttctaaa gcattgttag agattacttt aaaatcctct tatcaatttg ttcatattct
    22081 atttcaatct attatatata tcccatcaac tatgcatcat aatttaggta actcatactc
    22141 aataaaaatc aaaaagcaaa ctagaaagct aggcacagac tgctcaaaac accgttttaa
    22201 ggttgtggat agaactgacg aagtcagctc aaaacactgt tttgaggttg tggatagaac
    22261 tgacgaagtc agtaaccata cccatgacaa gccgacgctg acgtggtttg aagaaatttt
    22321 tgaagagtat tattctccat caaaggcatc tttaatatgg tcaaagaagc cttttttctt
    22381 tggatttact ttcaagtcac cagcagccgc gaattctttc aaggctactt tttggcggtc
    22441 gttcaagcct gtcggtgtta cgacattaac agtaacgtat tggtcaccaa ctgcaccgcc
    22501 acgaaggctc ggtgccccct tactacgtag gcggaatttc ttaccagtct gagttccctc
    22561 tggaataacc aattcaacat caccgtgaac agttggaata tctactgtat caccaagagc
    22621 cgcttggaca aagttgaggt tgagattgta gaagatagtc gttccttcac gttcaaactt
    22681 gtcgctagct tctacagaaa ctactacata caagtcacca taaggtccac cgttaaagcc
    22741 tgcttcacct tgaccagcga ggcgaatttg ttgacctgtt tccacaccag cagggatttt
    22801 cacatgtacg ctatgagctt gtttctcatg acctgttcca tgacaggttg tacatggata
    22861 tttgatttct tttcctcgac cgtgacagac atcacaggtt acttggcgac gcatcatacc
    22921 aagaggagtc tgcgtatcga cgttaatgac accagcgcca tgacagcgtc cacaagtgac
    22981 tggacttgtc cctggcttag caccagagcc attacatgta cgacagccag cttcacgatg
    23041 atacttaact tccttctcag ttccgaagat agcttcttca aaggtcaaat tgacacgata
    23101 ctggagatca tctccttggc gaggagcgtt tggattgcgc gaagaaccgc ctccgccgaa
    23161 gaaacttgag aaaatatcct caaaaccacc gaaaccacct gccccattga aaccgccgaa
    23221 accaccagct ccaccaaaac caccattggc gcctgcagca ccatactggt cataggcagc
    23281 acgtttttgg tcgtcactca aagtctcata ggcttcttga acttccttgt acttgtcctc
    23341 agcaccaggc tccttgttga tatctgggtg atattttttg gaaagcttac gataagcctt
    23401 tttgatttcg tctgccgaag cgtttttgga tacccccaga cgatcataaa attcagtatt
    23461 gttcatacaa gataccaaga ccgtaaaatt cgactgaaaa ataggaaatc tgacgctgga
    23521 gcgacgctcc tagacagatt tctctttttt ccgagaattt aggtcgggtt caattccttt
    23581 cttttatatt gatgagataa tactccagcc actcggccct atcccttttt atcgaaaaac
    23641 agaggctggg ttgcaacctc tgcaaataag taaattaact ttattttgat tttaaatatc
    23701 ctataagtac agaaatattt atatattttt gtctcttctt agtatttgcc tatataattc
    23761 caattgatca taagaaatac aactactgtc caaatcatac caattatgaa cggtattaca
    23821 aatttatatg tttgtatttc taatgatatc atagctgata ctatcgccga tacaaatgca
    23881 gacaataata ctgaggtaac aaaattcctt accgcttcat catttttgat acaactatta
    23941 tgttcagcta atataatttc tctatatcta gtcaaacatt ctactggaat attatctata
    24001 aaaccttttg atgttctata gttatcagct tgattacaaa aatcattata aacttttcgt
    24061 gtatttttta gatactcttc atccaataca ctcatcttac ttttccgtaa actctccgtc
    24121 tacgacgtca tcgcctgcgt ttcctgttgc ttgtgcgcct tctgctcctt cttgagcttg
    24181 ttgcgctgct gcggcttgtt cgtagagttt aacagcaagt ccttgagctt tttcgttcaa
    24241 tgcttcaagt tttgctttca tgtcgtccaa gttgttgtct tcttgagctt tcttaaggtc
    24301 atcaagggca gcttgggcag cgtcacgttc tgcgtcgaag cctttacctt cagtttcctt
    24361 gattgtcttt tcagtcgcaa agattgcttg gtctacttca ttacgaaggt ctacttcttc
    24421 tttacgtttc ttatcggctt cagcgtttgc ttctgcatct ttcatcatgc ggtcgatttc
    24481 ttcgtcagtc aaacctgagt tcgattggat gacaatagtt tgttcttttt gagttccaag
    24541 gtctttggcc ttaacagaca cgataccgtt cttgtcgatg tcaaatgtta cttcgatttg
    24601 aggaattcca cgaggtgcag ctgggatatc agtcaattgg aagcgtccaa gagtcttgtt
    24661 atctgctgcc attgggcgtt caccttgaag aacgtggata tcaacggctg gttggttgtc
    24721 tgctgctgtt gagaagactt gtgatttaga tgttgggatt gtagtgttgc gatcgataag
    24781 ttttgtaaat actccaccca ttgtttcgat accaagtgac aatggcgtta catcaagaag
    24841 gacaacgtcc ttgacatcac cagtaatcac accaccttgg atagccgcac ccatagcaac
    24901 tacttcatca gggtttactg atttgtttgg ttctttacca gtttcagctt taacagcttc
    24961 aacaacggca gggatacgag ttgaaccacc aacaaggata acttcgtcga tttctgacaa
    25021 gctcaaacct gcatctgaaa gggcttgacg aactggaact tttgtacgtt caacaaggtc
    25081 acgagtcaaa tcatcaaatt tcgcacgagt caaagtcatt tccaagtgaa gaggtccagc
    25141 ctcacctgca gtgataaatg gcaagctgat ttgtgttgaa gttacaccag aaaggtcttt
    25201 cttcgctttt tcagccgcat ctttcaaacg ttgcattgcc atcttgtcag tagacaagtc
    25261 gataccgttt tctttcttga attctgctac caagtggtca atgatttttt ggtcaaagtc
    25321 gtcaccacca agtttgttgt cccctgcagt tgacaatacg tcgaagacac cgtcacccaa
    25381 ttcaaggata gagacgtcga atgtaccacc accaaggtca aataccaaga ttttttcttc
    25441 tttgtcagtc ttgtccaaac cataagcaag agctgctgca gttggttcgt taacaatacg
    25501 ttttacttca agaccagcaa ttttaccagc gtcttttgtt gcttgacgtt gagcgtcgtt
    25561 gaagtaagcc ggaactgtga taacagcttt ggttactttc tcaccaaggt agtcttcagc
    25621 gtagcctttc aagtattgaa ggatcatagc tgagatttct tgtggagtgt attcttttcc
    25681 atttgcagaa actttttcag aagttcccat cttagatttg atagagataa ctgtatctgg
    25741 gtttgtgact gcttgacgtt ttgcagcatc accaacgatg atttctccgt ttttgaatga
    25801 gactacagat ggagttgtgc ggtttccttc tgggtttgcg atgattttgc tttcagttcc
    25861 ttcaagaact gcaactgctg agtttgttgt acctaagtca ataccgataa ttttagacat
    25921 gtgtttttct ccttgttagt taattttcta tttttctttt tgatactctt cttaagtggc
    25981 gacgccgtca gagcttgact tcgtcaatct ctttgactaa actttgagcc taaggtctca
    26041 aaatttgcgc aaatagcgcc acggcgaaga gtatcgtctt tggttcgctt cgctcactat
    26101 tacaaagcca aacatatttt atctttcttc atagtttatt gtcgtttcgg acaagttttc
    26161 tattatgtaa attgcgacac gaggagtcga aatcgatttt atttcgacga cgagttagta
    26221 aggaagctag acaaacgcca tagcgattgc cgttttctga cgagttgctt tagctaccgt
    26281 cagaattgcc gaatcgaaca cgccctaagc tctgagtaaa aaagatagat tttcttgtgt
    26341 tcatcgaaca cttcgtcaat ctcctatttt tactgcgggc tttgtatctt agttatacac
    26401 cactaccatt gctgggcgta ggatgcggtc atggagtttg tagccttttt gaaagacttg
    26461 ggcgatggta tctactgggt gttcatcgtc tgctgggaga gtttggatgg ccatatggta
    26521 gttatggtca aattcgccat ctgctgcgat ttcttcaatt ccttcttctt tcaaagcgtg
    26581 aatcaagctt tcttgcacca tccccaagcc cttcttcaca tcatctgtca aaccttcaac
    26641 tgcaagtgca cgctcaaggt tgtcaagaga tggtaagatt gcttttgcca agtcctggct
    26701 acgataacgt tgcaagtttt gacgttcttc attggcacgg cgttggatat tttgcatttc
    26761 tgcatgagcg cgaagatatt tgttttcgaa ctcatctgca cgttcatttg ccaagtccaa
    26821 ctcagacttt tcaggagttg tttcttcagc tgtttccaca acttcctctt cttgaacttc
    26881 ttctacttct tcatttttta tatcttgggc catgttcgcc tcctttaatg atttcaatct
    26941 taatgtactt cataatgatt actgctgagg tagcggtaaa aatctgtcaa cttcatggtc
    27001 aaaacacggt tgaccacatt gacttgattg attagctgtt ggtaatccag attaactgga
    27061 ccgataatgg ctagaattcc aactccccga taaggaatga ggaacttact actaatcacc
    27121 gctaggtcag ctaaacagga ctcttgaccg tctgcaacac gaacattttg catctgatcc
    27181 tcacgcaacc cctcacgaat ctccaaggcc accttttgcg gttggtcaaa gaactgatag
    27241 gctgctagat tggcaaaatt caagagatgg accttgcccg ccatcacaat gttttcgttg
    27301 aacatttcct taaagatgtg ttcaaagaga tcgatgacat tatccgttgt tgtaaagtaa
    27361 cgctggataa tctgcggaat ctccgtccga atcttgtagt gaatatctaa aacggtgtga
    27421 ccgaggaaac gttcctgaat gatgctcttc agtttcagca aatcctcctg caagaagttc
    27481 cttggaatca gaaactgact agtaaccgtt cgcgactcgt ctagggtaaa taccgccaag
    27541 gctgtatgtt gccccaaaac aacgatatca aaggctgtca aacgttgcct gctcggctca
    27601 acatccagtg ccactaccgt acagccactc aggtctgtta gcaagttagc agcctcttgc
    27661 agaatatcct ccaatttgaa gaattcttga tcaaaggctt tgacaatctc atatacctca
    27721 ttttcagcca gtcggtcaaa atccagtgag tgtttcacat agtactgaaa accagcaaca
    27781 cttggcatcc gaccgcttga agtatgagcc ttctcaagca acccttgttt ttctagttcc
    27841 gccatgtcat tacgaatggt tgcactgcta gagttaatag actcttgcaa ggtttttgat
    27901 ccgacaggtt cgtgcgtttt ggtaaagatg tcaataatca gatttaaaat atcctgctga
    27961 cgctctgtaa ccatctcatc actcctctct gtctgatcaa ttagcactca atgcatccaa
    28021 gtgctaactt atgattctat tatacaccct tagaagagaa tgtcaagaca aaaactcaaa
    28081 aaattagcac tcttttatca agagtgctaa aaatcaatct gaagacctag aaccttgcta
    28141 ttcatctact cggtatttcc tttttagtct gaaataacca tgaattagat aagaaatcag
    28201 aaaagcatat aaaacaaaaa taagaaagaa agacatagaa agatcctctc taaacaaact
    28261 catacaaaca acaagaccac ttaagaaagt agctgtacag gaacgaagtc tagatcgcat
    28321 aacttcccat ttgagcccct tactcatata gacttgatcc tctggaagta aatcagaaga
    28381 cgatttacga agaatcgaac aagaacctgc tcctatcaat tcccaacctc tatctctaaa
    28441 atcttgcagt tcatgcttat attttttaag aaatctagaa tcatagatac ggtagatgac
    28501 atcgtctggt tgacatcggt caaaatagaa caaaccaaaa cgactcgttc tatacctcca
    28561 acctttcaaa tgcatctcat gtaaatattc ttcttccttg tccaaatcaa caatagtgaa
    28621 aatccgaaat tctactctgc tattcattgt cttaccccaa aattagaaaa catgcctggc
    28681 gttatttatt agataattct ttccactttt gactcaatct ccaaaaaata taagaaatct
    28741 gaatcgcaaa aactatcaat aaaacccaat ctattatgaa agtcaaaaac acttcccaac
    28801 tgaaagaact acctccagtg acaaactttg agaaaaacgg tagtagagct aaaaagagaa
    28861 gcaaaatagg aagcatccgc attgttaaaa tccttttggc ataaaaaaat ctttatttaa
    28921 acgaaaatat tatggcaaaa tttacgccag tttttgaacg gctgatgtag atattttata
    28981 ctttcaaaat gtttaaatgt gattatttat ttttgaaaaa tagctcacca gcccgactga
    29041 aagtgcttat aaatgataat aagtcgcctg ccgaaaacag cgaaaaatag cggtgttatg
    29101 cggagataat ctgacgcgat gcgaaagtat attgcatact tattttcaac aatttagcag
    29161 agtattttta taagtgtgat ataatagaag tataatttgt tctgatagtt tattttatgg
    29221 agaagtagat ttttagaatg cggagggttc aatatggttg agtttataaa gtctaagaaa
    29281 gaaatgagtg aggaggatat taaagcaaat ttcatcactc ctgctattgt atccaaagga
    29341 tggaaaaatg gtgagcatat cgcttacgaa gaatacttca ctgatggtcg aatcgaagtt
    29401 agaggagata aggctcgtcg taaagaagga aaaaaatcag actattcact gtattaccaa
    29461 tttggaactc gaattgcaat tgttgaggca aaggataata aacacagcgt tcgagcagga
    29521 ttacaacaag ctattgaata tggagagatt ttagatgttc catttgttta ttcttcgaat
    29581 ggtgatggct ttattgaaca cgaccgtatc acgagagaag aacgtgagct ggagttagac
    29641 gaattcccta ctcgtgaaga attattttct cgtatgacga aggaaaaagg attgacgtac
    29701 gaaattacag aagctatctc aactccatac tatacagacg ccttctcaat gaaaacgcca
    29761 cgctattatc agcaaatagc tatcaaccgt actattgaaa cagttgccag aggacaaaaa
    29821 cgagtaatgt ttgtgatggc aacaggaacg gggaaaacgt tcatggcttt tcaaattatt
    29881 catcgccttc gaaaagctgg tttggctaaa cgagttttat tcttagcaga tagaaacatc
    29941 ttagtagacc aaacgatggc tgaagacttt aggccattcg aaaaggtaat gacgaaaatt
    30001 acaccaaaac ttttgactgc tcctgaaaaa ttaaattctt ttgaaattta tctagggctt
    30061 tatcagcaac taactggtga agatggaact gaaacacatt atcaaaaatt tgacaaagac
    30121 ttctttgatt taatcgtaat tgatgaagcg caccgtggtt cagctaagga aaacagtaac
    30181 tggcgtaagg taattgatta tttcagttct gcgacacaga ttgggatgac cgctactcct
    30241 aaagaaacca agaatgtttc caatacggaa tactttggtg agccaatcta tacttatagt
    30301 ttaaaacagg gaatcgagga tggttttttg gctccatatc gtgttatgag ggttaattta
    30361 gatgtggatg tggatggtta tcgtccagaa actggaaaag ttgatgctaa cggacaatta
    30421 atagaagata ggtactacgg caggaaagat tttgataaaa ccattgtcat tgatgataga
    30481 acgcaaagag ttgccaagtt tgtttctgat tatatgaagc aaaacaatgc acgatttgat
    30541 aaaacaattg ttttttgtgt tgatattgac catgccgagc gaatgcgtgc tgcacttgta
    30601 aaagagaatc tagacttagt ccaagaagac tatcgttatg tcatgcaagt aactggtgac
    30661 aacgctgaag gaaaagctca actggataac tttatggatg tcaattctaa ttttcccgct
    30721 attgtaacaa cgtctaaatt attaacgaca ggagttaatg ctaaaacatg tcgtttgatt
    30781 gttttagact ctaatatcca atccatgact gaatttaaac aaattattgg tcgtggcaca
    30841 cgtctttatc ctcaaaaggg gaaagaattt tttacgatta ttgattttcg aaatgttacc
    30901 aatttgtttg ctgaccctga ttttgatggt gatccagtga aggtgctgga aacaggtgcg
    30961 aaaacagtca gtggttctac gcccggtttc gtagatgagg aaggtgaccc agtagaaaaa
    31021 tatatcgtta cagacaagca ggttaccatt cttaattcta ctgttcaagt attggatgaa
    31081 aacgggaaac tgattaccga aagcctgacc gactacactc gaaagaatat cttaggtagc
    31141 tacgccactt tgaacgattt tatcacagtt tggcatacgg cagataagaa gaagcttatc
    31201 ttagacgaac tttataaaaa aggagtttat ctagatgcta ttcgagagtc ggagggaata
    31261 tcagaacaag aaatcgatga ttttgattta ctcctaaaac ttgcctatgg tcaaaaagaa
    31321 ttaaccaaaa cggaacgtat caataaactc aaacaaagcg gatatttata taaatatagt
    31381 gaggaagcgc gtgctgtttt ggaaatttta ctgaacaaat acatggataa aggtattgga
    31441 gaactcgaaa gcattgaaac attaaaactt ccagaatttc agatatatgg tggaaccttc
    31501 aaaatcatca atacttattt tggagataaa aaacgatatt tacaagcaat taaagaattg
    31561 gagcaagagc tatttacagt agcttaatga aaggaaagta tgtcaattac atcatttgta
    31621 aaaagaattc aagatatcac tcgaaacgat gctggtgtta atggtgatgc tcaacgtatt
    31681 gagcaaatgt cttggttatt attcttaaaa atttatgata gccgtgaaat ggtttgggaa
    31741 ttagaagaag acgagtatga gtcaattatc ccagaggaat taaaatggcg aaattgggct
    31801 catgctcaaa atggggaacg ggtattgaca ggcgatgaat tacttgattt tgtcaataac
    31861 aagttattca aagagttgaa agagcttgaa ataacttcaa ttatgcctat tcgaaaaacg
    31921 attgttaaat cagcttttga agatgcgaac aactatatga aaaatggcgt cttgttacgc
    31981 caagtcatca atgttattga tgaagttgat ttcaatagcc ctgaagatcg tcattcgttt
    32041 aatgatattt acgaaaaaat tcttaaagat attcaaaatg ctgggaactc aggagaattt
    32101 tatacgccac gtgcagcgac tgattttatt gccgaagttc ttaacccaaa acttggagaa
    32161 tcaatggcag accttgcttg cggaacagga ggcttcttga cttcgactct gaaccgttta
    32221 agtagtcaac gtaaaactag tgaagatacc aaaaaatata atacagctgt ttttggtatt
    32281 gaaaagaaag catttcctca tcttttagca gttacaaatc tgtttcttca cgaaattgat
    32341 gaccctaaaa ttgttcatgg aaatactttg gagaaaaatg ttcgtgaata tacggatgat
    32401 gaaaaatttg acattattat gatgaatcca ccttttggag ggtcagaatt agaaacaata
    32461 aaaaataact ttccagcaga attacggagt tctgaaacag ctgatttatt tatggctgtc
    32521 attatgtatc gtttgaaaga aaatggtcgt gttggagtta ttttacctga tggttttcta
    32581 tttggtgaag gtgtaaaaac tcgcttgaaa caaaaactgg tagatgagtt caacttgcat
    32641 acgattatta ggttgcctca tagtgtcttt gcaccgtata caggaatcca tacgaacatt
    32701 cttttctttg ataaaacaaa gaaaacagaa gaaacttggt tttatcgttt agatatgcca
    32761 gatggttata aaaatttctc gaaaactaag ccgatgaagt cagaacactt caattctgtt
    32821 cgtgactggt gggaaaatcg tgaagagatt ctggaaggta agttctacaa atctaaatca
    32881 tttacaccta gtgaattggc tgagttgaat tataatttag accagtgtgg ctttccaaaa
    32941 gaggaagagg aaatcttaaa tccctttgag ttgattcaga attatcaagc ggaaagagca
    33001 actttaaatc ataagattga taatgtatta gctgatattt tgcagttgtt ggaggacaaa
    33061 taatgacacc agaacaactt aaagcaagta ttctccaaag agcgatggaa gggaaattag
    33121 tgccgcaaaa tcccaatgac gaacctgcaa gtgaattatt aaagagaatt aaagctgaaa
    33181 aagaaaaact tatcagtgaa ggaaaaatca aacgagataa aaaggaaact gagatatttc
    33241 gtggtgatga tgggaaacat tatgggaagt ttgctgatgg aagcactcaa gaaattgatg
    33301 ttccttatga tattgctgat acttgggagt gggtgaggtt ttctacattg gttgaaattg
    33361 tcagaggtgg ctctccacga ccaatcaaag attatcttac ttctgaagta gatggaataa
    33421 attggataaa aataggtgat actgaaaagg gtgaaaagta tataaataat gttaaagaaa
    33481 aaatcaaaaa atcagggctt aacaaaacta gatttgtaaa aaaaggtaca tttttgttaa
    33541 ctaattctat gagttttggt agaccttata ttttgaatgt tgatggtgca atacacgatg
    33601 gatggttggc tatttcgaac tatgaaaact cattaaataa agattaccta ttctatattc
    33661 tttcatcaaa tgtagtttat tctcaatttc tatctctaat tagtggagct gttgtgaaaa
    33721 acttgaatag tgataaagtt gcttctattc ttatccctct ccctccgcta gctgaacaac
    33781 aacgcataat agaagcaatc gaatcagctt tagaaaaagt agatgaatat gctgaaagtt
    33841 ataatagact agaacagcta gataaaaaat ttccagataa actaaaaaaa tctattcttc
    33901 aatatgctat gcaaggaaaa ttagttgaac aagacccaaa tgatgaatca gtcgaagttt
    33961 tacttgaaaa aatacgagca gaaaaacaaa aactatttga agaaggcaag attaaaaaga
    34021 aagatttgga catttctatt gtttcccaag gagatgataa ctcttattat gggaataaag
    34081 acgaaacaac ctcttatcct atatatgaaa tcccagaatc atggagatat attaaatttg
    34141 ctagcctagt taacttcaga ataggaaaaa ctcctccacg cagtgaagct actttttggg
    34201 gaactgaaat accttgggta tctatatcag atatgcctat atctggttat gtaactaata
    34261 cgagagaatc tatttctaaa ttagcattaa aatcaaagaa aatagacatt tcacctaaag
    34321 gaactttatt aatgagtttt aaattatcta ttggaaaagt tgccatattg gatattcctg
    34381 ccactcataa tgaagccatt atatcgatat tcccctatgc taataaagaa aatattatca
    34441 gagattactt aatgatattt ttgccactta tctctacttt aggtgattca aaagatgcta
    34501 tcaaagggaa aacattaaat agtaccagta tctccgaatt attaatccct atttctaatc
    34561 atgaagagat gaaaagaatc atttccaaag ttgatttact ttttcaaaaa gtttctcaac
    34621 tttttgagta ataagttcct gttcctcaaa aggagctaac ggaattaata gctcgcttag
    34681 tgtagtttta ggaatattat ataaagcttg acctgatagt ttagttattg ctttcaattg
    34741 tttataaaat aacggagagg acaagttaaa taatagaaat tttgaaataa tctctgaact
    34801 ttcgaatggt gttaattgga aaataaatcc accagccaca acaccatcat agtctttatc
    34861 gattcttgca aactttccaa tatgttctaa agaggttgat acaggtgtta ttagctgatt
    34921 atgttttaaa taaacttgct cagaggagat gaattgtgta tcaatgtagt aatcgttatc
    34981 caacagagaa aattctaaag gcttaatatt accaccacgt ataattctaa cacctttatt
    35041 attaatgctt aaatcgccct tcttgtaaga aagacctgta ttcattgaaa aaatatcttt
    35101 tatttttata acaacccaat tcataggtat attcccataa taagagccat aatcacaaaa
    35161 atagcaggta gtcagtttga ccacctgtta ttttttacca attaacaatt ttatctacaa
    35221 tattttgttg ttcagtagct gttttcctta gataaattcg agtagtttct atactttcgt
    35281 gtcccatcaa atctgcaagc aaggcaatat cattatactt cgctaaaaaa ttcttagcaa
    35341 ataaatgcct aaaagaatga gggtaaatta ctttaggatt cattttgtat ttatcagcat
    35401 aattttttaa ctgttgagca actcctcttg ctgtaattgg ttcgttaaat ttattcaaaa
    35461 ataaataacc acttcggcga ttttctgatt ctaaccaact aagacaacta tttcttaatt
    35521 ttttaggaat gtacagtcta cgaattttac caccttttga gtaaatgtca aaataaccga
    35581 tttctacatg ctctactttt agtttaataa gttcacttac acgagcccca gttgcaccta
    35641 aaaaccaaac gacaaaatgc cattttaaaa taccatcttt tttcaaacta cgtttaagaa
    35701 aaaggtaatc agcatggcta atgacatctt ctaaaaacgg tttttgctgt actttgacaa
    35761 attttaattt caaatcatca tgaccaataa aagccagata tttatttact ccttgtagtc
    35821 gcaaattgac agttttaggt ttaaaattgt ctaataaata tcctttgtat tcaaataaat
    35881 cttccatttt gagttcgtaa ttctccaaga aaaatcgaac accataaagg tacgaacgca
    35941 cagtattttc agctaaacca gctttcttca aatgtaattc aaaatctttc aacgtaaaac
    36001 tcctatctta tgtttgatag aaattccacc gcacgtaaaa ctattatact aaattagtgc
    36061 gtcaatatgg gcgaaaaatt gttcgatttt atcaacgatt ctggattgtt caggaagggg
    36121 tgggaggggg attaaatatt cttttatagt tttcgttaat aattcttttt gttttgtact
    36181 acccgacgct ttttcttcaa taactgactg aacaatagga gaggaaagaa aattatagat
    36241 gaaatggcaa ttaataaccc ccgataagac tcttataact gtaacatggc tatctgcaac
    36301 agcccaggca taaggatttt tattttcatg gtaaatagct aatcgtccta acgtacctag
    36361 acctgttgaa ttccacatta aatcaccatc ccttagtaat ctttctttct ggtaactatg
    36421 aactgtttcg ggatcaataa atcttgctaa gtcaatagaa aagccagacc attgattaca
    36481 tttctgagca atcacagggt atataggaat atttgaatat tttggagact tccctctttg
    36541 aatgtaggag gttatatcgt ttaacctcac ccattcccaa ctttctggta tttcacaagg
    36601 tacttcctca taataagagt tatcatctcc ttgggaaaca atagaaatgt ccaaatcttt
    36661 ctttttaatc ttgccttctt caaa
//
LOCUS       DAVLVZ010000018        34062 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000018 DAVLVZ010000000
VERSION     DAVLVZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34062
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.018"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(54..1115)
                     /locus_tag="VYN77_000820"
     CDS             complement(54..1115)
                     /locus_tag="VYN77_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1558302.1"
                     /translation="MNKRVQAFLAKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLFPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            complement(1108..3939)
                     /gene="uvrA"
                     /locus_tag="VYN77_000821"
     CDS             complement(1108..3939)
                     /gene="uvrA"
                     /locus_tag="VYN77_000821"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1558303.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALHIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            4071..5015
                     /locus_tag="VYN77_000822"
     CDS             4071..5015
                     /locus_tag="VYN77_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1558304.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRAYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            5027..5710
                     /locus_tag="VYN77_000823"
     CDS             5027..5710
                     /locus_tag="VYN77_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1558305.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            5965..6996
                     /locus_tag="VYN77_000824"
     CDS             5965..6996
                     /locus_tag="VYN77_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1558306.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(7133..7810)
                     /locus_tag="VYN77_000825"
     CDS             complement(7133..7810)
                     /locus_tag="VYN77_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1558307.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(7807..8370)
                     /locus_tag="VYN77_000826"
     CDS             complement(7807..8370)
                     /locus_tag="VYN77_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1558308.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            complement(8380..8973)
                     /gene="ruvA"
                     /locus_tag="VYN77_000827"
     CDS             complement(8380..8973)
                     /gene="ruvA"
                     /locus_tag="VYN77_000827"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1558309.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKIAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     regulatory      9130..9244
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9378..10478
                     /gene="ribD"
                     /locus_tag="VYN77_000828"
     CDS             9378..10478
                     /gene="ribD"
                     /locus_tag="VYN77_000828"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1558310.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGSHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMTMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     gene            10463..11098
                     /gene="ribE"
                     /locus_tag="VYN77_000829"
     CDS             10463..11098
                     /gene="ribE"
                     /locus_tag="VYN77_000829"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1558311.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDANAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            11118..12323
                     /locus_tag="VYN77_000830"
     CDS             11118..12323
                     /locus_tag="VYN77_000830"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1558312.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYRITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            12324..12791
                     /locus_tag="VYN77_000831"
     CDS             12324..12791
                     /locus_tag="VYN77_000831"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1558313.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(13116..15065)
                     /gene="mutL"
                     /locus_tag="VYN77_000832"
     CDS             complement(13116..15065)
                     /gene="mutL"
                     /locus_tag="VYN77_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1558314.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLASA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            15421..15861
                     /locus_tag="VYN77_000833"
     CDS             15421..15861
                     /locus_tag="VYN77_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1558315.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            15858..16316
                     /locus_tag="VYN77_000834"
     CDS             15858..16316
                     /locus_tag="VYN77_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1558316.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            16626..17888
                     /locus_tag="VYN77_000835"
     CDS             16626..17888
                     /locus_tag="VYN77_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1558317.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(18181..18651)
                     /gene="nrdI"
                     /locus_tag="VYN77_000836"
     CDS             complement(18181..18651)
                     /gene="nrdI"
                     /locus_tag="VYN77_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1558318.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(18729..19262)
                     /locus_tag="VYN77_000837"
     CDS             complement(18729..19262)
                     /locus_tag="VYN77_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1558319.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            complement(19314..20600)
                     /locus_tag="VYN77_000838"
     CDS             complement(19314..20600)
                     /locus_tag="VYN77_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1558320.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            complement(20612..22258)
                     /locus_tag="VYN77_000839"
     CDS             complement(20612..22258)
                     /locus_tag="VYN77_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1558321.1"
                     /translation="MKHFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            complement(22260..22874)
                     /locus_tag="VYN77_000840"
     CDS             complement(22260..22874)
                     /locus_tag="VYN77_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1558322.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLVGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            23009..23578
                     /locus_tag="VYN77_000841"
     CDS             23009..23578
                     /locus_tag="VYN77_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1558323.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPHFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            complement(23607..24299)
                     /locus_tag="VYN77_000842"
     CDS             complement(23607..24299)
                     /locus_tag="VYN77_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1558324.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(24301..25362)
                     /locus_tag="VYN77_000843"
     CDS             complement(24301..25362)
                     /locus_tag="VYN77_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1558325.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            complement(25355..26728)
                     /locus_tag="VYN77_000844"
     CDS             complement(25355..26728)
                     /locus_tag="VYN77_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1558326.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            complement(26828..27682)
                     /locus_tag="VYN77_000845"
     CDS             complement(26828..27682)
                     /locus_tag="VYN77_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1558327.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            complement(27836..28666)
                     /locus_tag="VYN77_000846"
     CDS             complement(27836..28666)
                     /locus_tag="VYN77_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1558328.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKDLYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            complement(28770..29093)
                     /locus_tag="VYN77_000847"
     CDS             complement(28770..29093)
                     /locus_tag="VYN77_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV1558329.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLAF"
     gene            complement(29083..29739)
                     /locus_tag="VYN77_000848"
     CDS             complement(29083..29739)
                     /locus_tag="VYN77_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1558330.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTLVEMGLMSLFVYAGSAQFAML
                     ALIAVKAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAILFATLLGCSMGVVLDGQ
                     "
     gene            29810..29887
                     /locus_tag="VYN77_000849"
                     /pseudo
     CDS             29810..29887
                     /locus_tag="VYN77_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(30194..31420)
                     /locus_tag="VYN77_000850"
     CDS             complement(30194..31420)
                     /locus_tag="VYN77_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1558331.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLFVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKIALYASLLLQLLLLVGFATAHFGLILIFSFLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(31492..32157)
                     /locus_tag="VYN77_000851"
     CDS             complement(31492..32157)
                     /locus_tag="VYN77_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1558332.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(32225..32425)
                     /locus_tag="VYN77_000852"
     CDS             complement(32225..32425)
                     /locus_tag="VYN77_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEV1558333.1"
                     /translation="MLNLQFAETMELTEAELETVYGGECGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            complement(32725..33588)
                     /locus_tag="VYN77_000853"
     CDS             complement(32725..33588)
                     /locus_tag="VYN77_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1558334.1"
                     /translation="MIERMELGEFYKELRLARKLKQSDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRKVVDRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
BASE COUNT        10360 a         7416 c         6543 g         9743 t
ORIGIN      
        1 ttaatttttt ttgacttttt tcgtcatttt cgtacttttt tcttgacaaa caactaaatg
       61 actatcaact cttttggagc tagggtcaat aattcacaac ctgtctctgt aatcaggata
      121 tcatcctcga tacgaacgcc atatttgcct tcgatataga tacctggttc atcggtcaag
      181 gccatacctg tcttaatagt ttctgtagaa gtctggctaa agtagggttc ctcatggata
      241 tccagaccaa taccgtggcc aatgccgtga gtaaagtagt cgccataacc tgcctcaatg
      301 ataatatcac gagggatttt gtcaaagtca cggaaaccta agcctgcctt agcttggtca
      361 atcaaggctt ggttagcttt tagaaccgta ttgtaaatct ctgcctgctc atcgctaaca
      421 tgccctagat agatagtccg ggtcatatca ctgacatagt ggtcatagag acagccgaag
      481 tccatggtaa tggcttctcc caactccact ggtttgtgca ttggatgggc atggggttta
      541 gaagaattga taccgctagc taggatcgta tcaaaagata agccagatgc tcccaactca
      601 cgcatgcgga agtcaaggaa gttggcaatc tcaatttcag tttttcctgg tttgataaag
      661 tcaagcgcat cgcggaaagc ttggtctgag atagaacaag ccttgcgaat cgctgcaatc
      721 tctgcctcat ccttaatcat acgaagacct tccacaaact gagtttgcgg aaacaagtcc
      781 aaacctgcaa aagctgcctg catacggtgg taataagaca ctgaaatctc atcttcaaaa
      841 ccgatacgag tcaagcccat gtccttaaca attcctgcaa tgacagccaa ttcatcacga
      901 tcagccacaa tctcaaaacc actggtttct tgcttagctg cgatgatata gcgagagtct
      961 gtcactaaga cctgacggtc acggctgata aagactgttc cgtttgagcc ccaaaaacca
     1021 gtcaaataat agacgttttt aagattgttg atgatgacac catctagttc tttttcttgc
     1081 attttagcta gaaatgcttg tacgcgttta ttcatgatgt aactttcctt tcaaatagtg
     1141 tcctgtatag ctggcttcgt tggcagctac ttcttctgga gttcctgtta cgatgatggt
     1201 tccaccaccg acaccgccct caggtcccaa gtcaatgata tggtctgccg tcttgataac
     1261 atccagattg tgctcgatga cgaggactgt attgccatcg tctacaaagc gagctaaaac
     1321 cttgagcagg cgagcaatgt cctctgtatg aagccctgtc gtcggctcat ccagaatgta
     1381 gaaagatttt cctgtcgagc gtttgtggag ttcgctagct aacttcatac gttgggcttc
     1441 tcccccagaa agggtggtag ctggctgtcc caaggtcaca tagcctagcc ctacatcctt
     1501 gatggtctgg agtttgcgtt gaattttcgg aatgtgttgg aaaaattcta ccgcatcgtt
     1561 gaccgtcata tccaagacct gcgaaatatt cttttccttg tagtgaactt ctagggtttc
     1621 actgttatag cgggttccgt ggcaaacttc acaagccaca taaacatctg gcaagaagtg
     1681 catctcaatc ttgataatcc cgtcacctga gcaagcttca cagcgacctc ccttgacgtt
     1741 gaaactgaag cgccccttct tgtagcctcg aatcttggct tcatttgtct gagcaaaaag
     1801 gtcacgtata tcgtcaaaaa ctcctgtata ggtagctggg ttagacctcg gcgtccgtcc
     1861 gatagggctc tggtcaatat caatcaaacg gtcgacatgc tcaatccctg taatagtctt
     1921 aaacttacca ggtttgtctg aattacggtt gagcttctgg gcaatggctt ttttgagaat
     1981 gctgttgatt agagtcgatt tccctgaacc cgacacacct gtcactgcga taaattttcc
     2041 tagtggaaag cgagccgtga cattttgcaa gttgttctca cgcgctccta tcacttcaat
     2101 aaaacgacca tttccgacac ggcgctcttc tggtactggg atgacacgtt tgcctgacaa
     2161 gtactgacct gtgatagact tgctgttgcg agccacttgc ttaggtgtac ctgctgcaac
     2221 aatctcacca ccaaaaacac cggcaccagg accaacgtca atcagataat cagcctcacg
     2281 catggtatct tcgtcgtgtt ccaccacgat aagagtattg cccaagtcac gcatcttttt
     2341 cagactggca atcaggcgat cattgtccct ctggtgaaga ccgattgacg gctcgtctag
     2401 gatatagagg acacctgata ggttggaacc aatctgggtt gccaaacgaa tgcgctgact
     2461 ttccccacct gaaagggttc ctgctgaacg tgacagggtt agatagttaa gacccacatt
     2521 attaaggaag gtcaaacgat ccttgatttc cttgagaatg ggacgagcaa tgatggcttc
     2581 attttcagac aaagttaact ggctcaccaa gtccaagtgg tcagcgatag acaggtctga
     2641 gatttctcca atatgtggcc cttgctggcc gcccacacgg acagacaagg cctggtcatt
     2701 gagacgatag ccttgacagg ttccgcaggt cagctcattc atgtagagac gcatctgagt
     2761 gcgagtgtaa tcgctattgg tttcatggta acgacgtttg atattattga taactccctc
     2821 aaacggaatg tcgatatcgc gcacgccacc aaattcattc tcatagtgga aatggaattc
     2881 cttaccatct gacccataga gaatcaagtt cttatcttct tctgacaggt cctcaaaagg
     2941 cttatccata gccactccaa agactttcat ggcctgctct aacatgtttg gatagtagtt
     3001 ggatgagata ggattccaag gtgctagcgc tccctcacgt aaggttttgc tagcatctgg
     3061 cactaccaaa tcagtatcca cctccagctt gatgcccaag ccgtcacact cactacaaga
     3121 gccaaaagga gcattgaaag aaaagagacg aggctctaac tctgggacag taaaaccaca
     3181 aactggacag gcataatgct cagagaacaa caactcagag tcgtccatgg tgtcgataat
     3241 gacataacct tctgcaatat gaagggcagc ctcaatggaa tcaaagagac gactacgaat
     3301 gccctccttg ataacaatac ggtcaaccac gacatcgata ttgtgttgct tgctcttaga
     3361 caactctggc acttcggtca catcatagac ttccccatcc acacggacac gaacataccc
     3421 gtctttctga accttctcga taacactctt atgttggcct tttttcttgc ggatgacagg
     3481 agccaagatc tgcaagcgct ggcgttcagg taactccaaa accttatcaa cgatttgctc
     3541 cacagaagaa gcattgatag ctccatgtcc gttgatacag taaggcgtcc ccacacgtgc
     3601 gtagaggaga cgcagatagt cattgatttc agtcgtcgtt cccaccgtcg agcgaggatt
     3661 tttactagtc gttttctggt cgatggaaat agctgggctg agaccatcaa tggcatctac
     3721 atctggtttt tccatatttc ccaagaactg acgagcgtag gcggacaaac tctctacata
     3781 gcgacgttgt ccctccgcat agagagtatc aaaagccaga ctggacttcc ctgaacctga
     3841 caagccagtc acgacaacca acttgtctcg cggaatctcc acatcaatat tttttaaatt
     3901 atgggcacgc gccccatgaa tgacaatttt atcttgcatc tttgttcttt ctagtccatt
     3961 attgcttacc attataccaa aaaaagtgag attctattac ccaaaaggcc gattttgtag
     4021 tataatagta cagtgtgaaa aaatctgaaa aatgagaaag gataagggat atgaaacaag
     4081 tttttctctc tacaacaact gaatttaaag agatcgatac gcttgaaccg ggtacttgga
     4141 tcaatctcgt caatccgact caaaatgaat cactcgaaat cgccaacacc ttcgatattg
     4201 atattgccga ccttcgagca ccgctcgatg cggaagaaat gtctcgtatt accattgaag
     4261 acgagtatac cctgattatc gtagacgtgc cggtcacgga ggaaagaaat aaccgcgcct
     4321 actacgtaac catcccgctt ggtattatca tcactgagga aaccattatc actacgtgtt
     4381 tggaaccact acctgtcctt gatgtcttta tcaaccgtcg attgcgtaat ttctatacct
     4441 tcatgcgttc acgttttatc tttcaaattc tttatcgcaa tgcagagctt tacctaacag
     4501 cccttcgttc aatcgaccgc aagagtgaac aaatcgaaag tcaactgcat caatcaactc
     4561 gtaatgaaga attgattgag ctcatggaat tggaaaaaac tatcgtctat ttcaaggcct
     4621 ccctcaaaac aaatgagcgc gtgattaaga aattgaccag ttcaaccagc aatatcaaga
     4681 aataccttga ggacgaagac ctgcttgaag acaccctgat tgaaacccaa caggccatcg
     4741 agatggcaga tatttatgga aacgtcttgc attctatgac agagaccttt gcctctatca
     4801 tttctaacaa ccagaacaac atcatgaaaa ccttggccct tgtgaccatc gtcatgtcca
     4861 tcccaaccat ggtcttttct gcctacggga tgaactttaa ggataatgaa attcccctaa
     4921 acggagagcc aaatgccttc tggttaatcg tctttatcgc ctttgctatg agtgtctcgc
     4981 tcactctcta tctcatccat aaaaaatggt tctaagagga gttcctatgt ctcaaattga
     5041 tctacaaaaa ttaactaaga aaaaccaaga gtttgtccac attgctaccc aacaattcat
     5101 caaagatggg aaaacagacg ctgaaatcca gactattttt gaggaagtca ttccccaaat
     5161 ccttgaggag caatctaaag gtacaactgc ccgttcccta tacggcgcac caactcattg
     5221 ggctcatagc ttcactgtca aagagcagta cgaaaaagag catccaaaag aaaatgatga
     5281 tccaaaactg atgattatgg actcagctct tttcatcact agcctctttg cccttgtcag
     5341 cgccctcaca accttctttg cggcagatca agctttcggc tatggattga ttactcttct
     5401 attagttgga ctggttggtg gatttgcctt ctacttgatg tactactttg tttaccaata
     5461 ctatggacca gatatggatc gcagtcaacg tccacctttc tggaaatctg tactagttat
     5521 cctagcttct atgttccttt ggttgcttgt cttctttgca acaagcttcc taccagctag
     5581 ccttaaccca gtactggatc cattgccact agctattatt ggagcagccc tcctagccct
     5641 tcgcttctat ctcaagaaac gcttgaatat ccgtagtgca agtgcaggac caacacgcta
     5701 tcaagaataa gaaaacgata aaagcaactg caggtgcggt tgctttttca cttacttttt
     5761 tgagttgtac tcaatgaaaa tcaaagagca aactaggaag ctagctgcag gttgctcaaa
     5821 gcacagcttt gaagttgcag ataaaactga cgtggtttga agagattttc gaagagtatt
     5881 aaaagtattc ttctgaaatc ccacatagct ttctcttata ttttgtgata aaataggctc
     5941 aatctatttc taggaggata agatatggtt tctactattg gtattgttag tttatctagt
     6001 ggcattatcg gagaggattt tgtcaaacac gaagtggact tgggtatcca acgtctcaag
     6061 gatctgggac tcaatcccat ctttttgccc cattcgttaa aaggattaga ctttatcaag
     6121 gaccatcctg aagctcgtgc agaggatttg attcatgcct tttctgatga tagcatcgac
     6181 atgatcctat gtgccatcgg tggagacgat acctatcgct tgctacctta tctttttgaa
     6241 aatgaccaac tccaaaaggt tatcaaacaa aaaatttttc ttggcttctc ggatacaacc
     6301 atgaaccacc tcatgttgca taaattagga atcaagactt tttatggtca atccttttta
     6361 gcagacattt gtgaattaga caaagaaatg ctagcctata gccttcacta ctttaaagaa
     6421 ttgattgaga cgggaagaat ttcagaaatc cgccctagtg acgtttggta tgaggaacga
     6481 actgatttta gtcccacggc cctgggaaca cctcgtgtca gtcatacaaa tacaggtttt
     6541 gacttgttgc aaggaagtgc tcagttcgag gggaaaatcc tcggtggttg cctcgaatcc
     6601 ctctacgata tctttgacaa ctctcgatac gcagatagca cggaactctg ccaaaaatac
     6661 aaacttttcc ctgacttgtc agactgggaa gggaaaatcc tcttgctcga aacaagcgaa
     6721 gaaaagccta agccagaaga cttcaaaaag atgttgctaa ctttaaagga cactggcata
     6781 ttcgcggtca tcaatggact cttggtcgga aaacctatgg atgaaacttt ccatgacgac
     6841 tataaagagg cactattgga tatcattgac agcaatatcc cgattgtcta taatctgaat
     6901 gtcggccacg caactccaag agcaattgta ccctttgggg tccatgctca tgtagatgca
     6961 caggaacaag tcattctctt tgactataac aaataaagct gggaaaagtt tctcagcttt
     7021 tttctcggta tcattactgg cattttcatc acatattaca gtatattgaa actagaatag
     7081 tacacctcta cttctaaaac attgttagaa atcgatttgt cctgttctca tttcatttta
     7141 ctatactgat agctaaaatg attaaggaga ggctgttgct taacatgtga acaagtaggg
     7201 gatagtagat attctctttt tctttcacat aaataataga aaaggcaagg cctccaccta
     7261 agtaaccaac tgcaccaatc cactctgata gagccaaact gtgcatatga gtcagagcaa
     7321 aaacaagtcc taccagaata atgcttagta aacctgacaa ccgttcctgc aagtaatgca
     7381 ataagacctg acggaaaaat aattcttcta ccagaggtcc aatgacacaa gcaaaaacag
     7441 ctatcagtag tggttgttct tgaaaggtac tttgaatatt agactgattt agaccttgtc
     7501 catctagtcc cacaaactgc ttcaacatct ctgatacaaa ttcaaagaca acagtcatca
     7561 gaatgagaaa gagccatcct gttaagactc caaagaagaa ttttctttta gtctttctaa
     7621 tctccttcca ttgttggatc aatctatcct tgaacaagaa ggagccatat aaagctaaaa
     7681 ctacataaac aagataattt gcaatggcag atggcattaa agaagccagc aatttaacgc
     7741 catcaaacac aaagaggact gtatagagaa tcgcaaaaat cagaattccc ttatttttgt
     7801 tagacatcat ttaagacctt tccactcaca atcattctcg tggtcatcaa ctagccctgc
     7861 agcctgtaga aaagacaata cggcgactgg gcctgtgaac ttgaagtctc gttttttgag
     7921 atctttggct aatttctcag ataagggtgt tttagctggg gcttggcgat aatcaggaac
     7981 atcgttaacg acagttttcc cctcaacaaa agaccaaaga taggcatcaa aagagccgta
     8041 ctctgcctgt aactgtagaa aggcttgggc gttagcgcgt gtagcaaaaa tcttggctct
     8101 atttcgaatg atagctggat tctccagcat ggcttccaat tcagtgtcag tcatctctgc
     8161 gactgagtga atttgatagc tatgaaaggc ttctcggaaa gcttggcgtt tgttgagtac
     8221 cgtttcccaa gacaggcctg cctgataggt ttccatacac aacaactcaa acaacgcttg
     8281 gtcatcatgg aggggctggc cccactcctc atcatgatag gcgatgtaga gtgggttggt
     8341 catcttgacc cacgaacaac gttttgtcat tctctgctcc tatttgacca acattttaag
     8401 ggccgacttg atatagttct cagctgtatc tgtcgttcct tcaaagaatt tcttgatttt
     8461 cttgagctct gttgccttgt agcccagagc caacatggct tccatagctt cttccaattc
     8521 ttggttttca gcacttgctt gcactgcgat cttggcagga aggtcatctc ctgcaactac
     8581 taccttgcct tccaagtcca gcaccatctg ctgggctgtt ttcttgccaa ttttagggaa
     8641 cttggtcaag taggtgatgt tcttggtttc aatggcttga accaagccag cattgtcatc
     8701 agcagcgata atagcaagag ctgatacagg accaatccca gagaccgaaa tcagactaag
     8761 aaagagcttt ttctcatcct ctgagcgaaa tccataaagc aaatgggcgt cctcacgcac
     8821 aacctgatgc acataaatct gagcctcctg attaacctga cctgaatagg cataaggatt
     8881 ggccacatgc aggatataac caataccatt ggtttcaaga acaatgtatt tggcagtaat
     8941 tttggtaatg attcctttta aatatgcgta catagtgttc cttctctctt atttcgttta
     9001 tctagtatat catattttct ttatccgagg agcgagaatc gattatcaca aatacagtat
     9061 atcaaaaaca atcggtattt atctgttgac atattcaaaa ttaagtacta taatcatctg
     9121 taattgaata actatcttca gggcagggtg caattcccta ccggtggtat agcccacgag
     9181 ccgaaaggca tgatttggtg aaattccaaa gccgacagta tagtctggat gaaagaagat
     9241 aaaagtatat ttgtgctttt tgcgtgctct gaaatgatta cttgtcattt cagagcattt
     9301 ttgttaatcg cataagtttt cttttgtatg ccttgagtag tctcctattc aaggtatatt
     9361 tttttggagg tagtgagatg agcgattcaa aatatatgaa attagcaata aaactggcac
     9421 aaaaaggggc tggttacgtc aatcccaatc ctatggtcgg cgcagttatt gtaaaagata
     9481 atcacatcat cggacaaggt tatcatgagt tttttggtgg ctcacatgct gagagaaatg
     9541 ctcttaaaaa ctgtagagaa tcccctgtcg gagcgacgct ttatgtaaca cttgaaccct
     9601 gttgtcactt cgggaaaaca cctccttgta tagatgctat aatcgatagt ggtattacaa
     9661 gagtagtcat tggaagccta gactgtaatc ctattgtatc tggaaaagga gtaaagatac
     9721 ttgaggaaaa taatcttcaa gttactgttg gaattttaga aaatgagtgt cttaacttaa
     9781 taaaaagttt tagaaagtat attacccagc atgtacccta tgtttttatg aaatatgcaa
     9841 tgacaatgga tggaaaaata gccactaaaa caaatcaatc caaatggatt actgaagaag
     9901 aggcaagaaa gcatgtgcat cagttacgac accatgttag tgcaattatg gtgggagtca
     9961 atactgttat tcaagacgat cctttgctga catgtagatt ggaggaaggg aaaaatccta
    10021 tccgtatcat atgcgataca catttacgaa ctcctcttac ctctaaaatc gtaaaaacag
    10081 caaatgatat taaaacttac attgccactt cctctgaaga caaaaataaa atgaagctat
    10141 atcaaaatca tggctgtgaa atactttcca taaagaaaaa aggcaatcat atagacttat
    10201 cgagtttaat gcaacatcta ggaaacatgc agattgatag cctagttcta gaagggggca
    10261 gtctaatgaa ttggagtgct ttggaacaac aaattgttga tgagctgaaa atatatattg
    10321 caccaaaaat ttttggaggc agtgccaagt ttcctgtcgg aggtgaaggc atttctttgc
    10381 caaatgacgc tattagattg aaaccttatg ctttttctca aataggaaat gactatctca
    10441 tagaaagtga agtgatttat ccatgttcac aggaataatt gaagaaatcg gaaaagttga
    10501 aagaatacag aaagactctc gtaattgtaa actatcaatt aaagcctcaa aaatattaac
    10561 ggatatccat ttaggcgata gtatagcagt aaatggtatc tgtcttacag ttactcattt
    10621 caatcatcaa tcctttacag ttgatgtaat gaatgaaaca tggagtcgaa cagctcttac
    10681 tctattaaaa catggaagtg aggtgaatct agaaagagcc ttatctgtca acggtcgact
    10741 tgggggtcac gtcgttacag gacacattga tggtacagga aaaatctcgt caataaaaaa
    10801 agatgctaat gctgtatggt atcaaatcaa cacacaaaaa gaaattttag atttaatagt
    10861 tgaaaaagga tctattacaa ttgacggcat tagtctgact gtcgctaaag tctccaaagt
    10921 aaacttttca gtatctgtta tccctcatac cttggaacaa accattctta agagtaaaca
    10981 agtcgggagt acagtaaatc ttgaaaatga tatcttaggt aaatatgtgc aaaaactgat
    11041 ggataactct ccaaaatcag aaatatctaa ggaactatta tatcaaaatg gattttagca
    11101 gaaaggataa tcagccaatg gaatatcgaa aaatacaaga agcattagaa gcattgcaga
    11161 agggacgact tgttcttgtt atagacgaca aggatagaga aaatgaagga gacttaattt
    11221 gttctgcaca agcagctaca acagaaaatg ttaattttat ggctacttat gccaaaggat
    11281 taatttgtat gcctatgagc gaaagtttag ctaatcaatt aatgctttca cctatggttg
    11341 aaaacaatac agataatcat aagactgctt ttacagtttc aattgattat aaagaaacga
    11401 ccacaggtat ttctgccgag gagagaggac tgaccgcacg tatgtgtgta gctgaagata
    11461 taacaccctc tgattttcgc aggccaggac acatgtttcc tttaattgca aaaaaaggtg
    11521 gtgttctaga aagaaatgga cacacagaag caactgttga tttattaaaa ttagctggac
    11581 taaaagagtg tggcctatgt tgtgaaataa tgaatcatga tggcaaaatg atgagaacag
    11641 atgatttaat tcagttctcg aagaaacaca acattccact aattaccatc aaagaattac
    11701 aagaatatag aaaagtatat gatcagctgg tagaacgagt ttcaactgtc aatatgccta
    11761 ctagatacgg taatttcaaa gcaattagct atatagataa actaaatggg gaacatcatc
    11821 ttgctcttat tatgggaaac atagaggatg aagccaatgt attatgtcgg gtccactccg
    11881 aatgtttaac aggggatgtt ttaggctctt tacgttgcga ttgtggacag caattcgata
    11941 aagctatgaa aatgattgtt gagaatggtt cgggtgtctt actttacttg cgacaggagg
    12001 gacgaggaat tggacttatc aataaattaa aagcctatca tttacaagat caaggcatgg
    12061 atacgcttga tgccaatctt gcattaggct ttgaaggtga tttaagagaa tatcatattg
    12121 gagcacaaat gcttaaagat ctgggacttc agtcacttca tttactgaca aataatcctg
    12181 acaaggttga acagttagaa aaatatagaa ttaccatttc cagtagaata tcaatcgaaa
    12241 tagaagccaa tccttacgat agtttttatt tagaaacaaa gaaaaatcga atgggtcaca
    12301 ttttaaatat ggaggaaaaa taaatgaaca cttatgaagg taatttagta gcaaacaata
    12361 ttaaaatagg tattgttgta gcgagattta atgaatttat aacttcaaaa ttattatctg
    12421 gagcactaga taatctcaaa agagagaatg taaacgagaa agatatagag gtagcctggg
    12481 ttccaggagc ttttgaaata ccactgattg catcaaaaat ggcaaaaagt aaaaaatatg
    12541 atgcaattat ctgcttggga gctgtcatta gagggaatac aagtcattat gattatgtat
    12601 gtagcgaggt atctaaagga atcgcccaaa tcagtttaaa tagcgaaatt cctgttatgt
    12661 ttggtgtgct aacgacagat acaattgaac aagccataga acgagctggt actaaagcag
    12721 gaaataaggg ttctgagtgt gcacaaggag ctattgaaat ggtcaaccta attcgtacat
    12781 tagacgcata gaacagactc tacttttaga cgaataaaga aacttggcaa aatcgcaatt
    12841 taatactctg tgaaaatcat agagcaaact aggaaaccag tcgcagagtg gtaaaacaca
    12901 acttcgatgt tacagacaaa actggcaaaa ttagctcaaa acactgtttt gagattactg
    12961 ataaaactga cgtcgtttaa aaagagtttc gaaaagaatt aggaaaaaat gaagtaacta
    13021 tttccatgat aaaacgcata aaatcaaggt ttttgaacac ctgattttat gcgtttttct
    13081 gattttgaaa atttttccca gactttttta tacttttaat atttccccaa ctcacggaga
    13141 ctggtgtgat tttcctgaat acgtcggaac atcttttcca tatccgactt ggtaaaatgc
    13201 accaaaacag gacgtccgtg aggacagtta tagggattgt cacattgaga aagctgatag
    13261 aggagttgtc tagctgaatg atcatcaata cgatgattgg ccttgataga tcgcttgcaa
    13321 gacatcatga tagccagctc tgctcggtat ttcttgatag aaacttcctt ggtcaaaagg
    13381 agcatgtcgc acatctcata gatgcctgat tcaatctctt cttctgccat ccaaatagga
    13441 tgttcacgta gaataaattg attttctccg tactctgcta gaaagacgcc cacttcctct
    13501 aagagaggca ttctttcctt gagacgcagg gcatcatccg caggaaattc aaagatatag
    13561 ggcactagga gttgctgctg gctctggtca acattgccaa tgctttcacg gtactcctcg
    13621 tacttgaccc gttcctgagc agcgtgctga tctatgatgt aaagtccatc tcgcccttgg
    13681 gcaaagagat aagtcccgtg catttgtccg aaaaactcca actctgggaa gctggatgct
    13741 tcttctcgct ccagtttgtc ataagcctta tcgatgctag caagatctaa ctctggatgg
    13801 tctagctggt cgtagttagc aggctttctc tctgcaaaat gcagttttgc tggcttggtc
    13861 aatcggtcca aggtttcctt ggcaaacagg gtcaaatcct gcccttcatc agtcaattct
    13921 acctgataat cagctacttc agtttgacta ggtcttgacg gctcagtttt ctcatagtag
    13981 agcgtatttt ctttgagtgg gagaatagtt tgctccacct tttcacgatt gcgcacggtc
    14041 gatttggcaa gattttccaa ggcatctgga atcaaggttt gttccttgag actatttgca
    14101 atagcttctg aaaccagagt catcagttct ttttccttgg aaatccgcac ctcttgctta
    14161 gttggatgca cattgacatc cgctagataa gggtcgatat ggatgtgaat gacagccagt
    14221 ggaaaacgtc caaccataag cttgcttccg aaaccatcca aaatagcacg attgagcagg
    14281 aagttcttga tataacggcc attgatgaag aggctgatat aattgcggtt agcccgagtc
    14341 aactcaggca aggacacaaa acctgaaatt tcgaaatcta ggtcagagtt ctcaatttca
    14401 atcatcttct tggcactcgc caaaccgtaa atccctgcga ttgcttggcg cagttgacca
    14461 gtccctgctg tccgcgtcat ttccttgcca tcactaatca agctaaaaga aatctcagga
    14521 tgggccaagc ccagacggtt gacaatatca atgatatgag acaactccgc ttgctggctc
    14581 ttcatatact tgagacgggc aggcgtgttg aaaaagagat cctccacaca aaccttggtt
    14641 cccacaggac tagtcgctgg gatgacttcc tcaacttcac ccccacgtgc gactaacttg
    14701 gttccatgac tagcaccatc caccgccgtt aacagagtca agacactaac agacgcaata
    14761 gaaggcaagg cttcaccacg aaaaccaagc gtccgaatcc gaaagagatc tgcttgattt
    14821 tttatcttac tggtcgcatg gcgacgcagg gccaactcca cctcatcgtg ggcaattcca
    14881 tgaccgttat ccgtgatttg aaccttcttg agaccagctt cctcaatctc aatgataatc
    14941 tgactagagc ccgcatcaat ggcattttct accaactctt tgaccacact ggcaggacgt
    15001 tcaatgacct ctccagccgc gatttggttt gccagcatct ctggcaattc aataatatga
    15061 gacatctttc agcccctttc cgtatctatt ttcctagaaa ttgattagtg ctattatacc
    15121 atatttcaga aagaacagaa aagctccacc acataggagc ccaatctctc cacataggca
    15181 aagtcccttg ccaaggggca taaaatagtg gtatactcaa taaaaatcaa agagcaaact
    15241 agaaagctag gcgcagacag ttcaaaacac tgttttgagg ttgtagataa aactgacaaa
    15301 gtcaacaact atcctatggc aagacgacgc tgacgtggtt tgaagagatt tttgaagagt
    15361 attaataaag gtgtacaaga agtgagatta attttgtatg aaaaacaagg agagaaattt
    15421 atgaaagtag aactacagat tagtgagact tacgaggagg aaaagctgat tgtccaagca
    15481 cctcagccga cagataaagt ccagaaagtc atcgagttcg cagaaaatct ggaccaaaga
    15541 gaaaaaatca aagggaaaat cgatgatcag gtctatctag ttgagattgg caagattcag
    15601 cgcttctata tcgagaatcg gaaggttcta gcagaaaccg cgagtcagac ctacagcatt
    15661 gatttgcgac tctatcaggt tcttaaactc ttgccaacca attttatcca aatttcccaa
    15721 tcagaaatca tcaatatcga ttccatctct cacctcaagc tcacgccaaa cggtctggta
    15781 gaaattttct tgaaaaacga aagcttcacc tactcttcac gccgttatct aaaaaccatc
    15841 aaggagaaat tagaactatg aaaaaacaag tatttcacga tgcagctacc ggtgttctta
    15901 tcggcctcat cctctctatc ctcttttcac tcatttatgc accaaatacc tacgcaccac
    15961 taaatcccta ctctctcatc ggccaagtga tggatcagca tcaggttcac ggtgccctgg
    16021 tcttgctcta ctgcacactt atctgggcaa ccatcggtat gctcttcaac tttggcaacc
    16081 gcttatttag ccgtgactgg agcatgcttc gtgccactct gactcatttc ttccttatgc
    16141 tggctggctt tgtcccacta gcaactcttg ctggttggtt ccctttccac tggattttct
    16201 acctccagct cattatcgag tttgcgattg tctatctcat catctgggct attctctata
    16261 aaagagaggc taaaaaagta gatcacatca atcaactctt ggagcataga aaataggaac
    16321 ctcataataa gcaaatccga gaaagaaact tctcggtttt tctttatcct gctgcacctt
    16381 actatagtga aatgaaataa gaacaggata aatcgatcag gacagtcaaa tcgatttcta
    16441 acaatgtttt agaagcagag gtgtactatt ctagtttcaa tcctctatat cataacagtt
    16501 atttcagatc ttctttgtaa attttctatc ttttttcaaa atactcttgc atttttccaa
    16561 aattttttgt agaatataga ttacatatac agattattct gaaaattcaa aaaggagtat
    16621 ttatcatgtc gcaagctatt tccttaaatc aatcaacctg ggcaagcaag ctaaaagcaa
    16681 tgggacctgg aatcctaatg gcaactgccg ctgttggagg ttcccacatt gtatcctcaa
    16741 ctcaagctgg cggttcttac ggttggtctc tacttctctt ggtcatctta gccaatgtct
    16801 ttaaatatcc atttttccgt tttggtgctg aatacacagc tgatactgga aagactttgg
    16861 ttgaaggtta tgccgaaaaa ggaaaactct atctctggat tttctttatc ctcaatgtct
    16921 tttcggctat ggtcaacacg gctggtgttg ccattctgtg ctcagctatc atcgccagtg
    16981 ccttcccaat gattggactt agtattactc agtggtccct cattctcgtt gcaatcattt
    17041 gggctatgct actctttgga ggctacaaac ttttagacgg catggtcaaa tggattatgt
    17101 ctgccttaac cattgcgact gttctggcag ttatcattgc ggcggtcaag catccagaat
    17161 acagttctga ttttgtcgag aagacacctt ggcaaatggc agctctgccc ttcatcgtct
    17221 ccctcctagg atggatgccg gctcctattg aaatttcagc catcaattca ctttggtcag
    17281 ctgaaaagag aaagaccgtc aactttaaca cagaagacgc tctgtttgac tttaacactg
    17341 gttatattgg aacagctatc ctagccgtct tctttgtggc actgggagca ctgattcagt
    17401 atcctacagg gcaggcggtt gaagctgctt cagccaaata catctctcaa ttcgtgggca
    17461 tgtatgcctc tgttcttggc gaatggtccc gttacttgat tacctttatt gccttcctct
    17521 gtatctttgg aacagttata actgttatcg atggctattc tcgcgttaat caggaatctc
    17581 tccgactgct aatcagtcaa aaagaggaca atcgtaaatc tttgaacatc tggatgacca
    17641 tcactgctat catcggtatc gtcattatca agttcttcgc tggtcaggtt tcaaccatgc
    17701 tccgctttgc catgattgga tctttcctga caacaccttt ctttgctctt ttgaattacg
    17761 ccttggtaac gcgtgaaaac aaaaatcttc cttcttggct caaacacctt gccattgcgg
    17821 gattgatttt cctctttggc ttcgccatct tctttatcta cgcactcgca atcggaaaag
    17881 cagggtaagg gacaaccgcg agatgaagat aaggtttcat ttcaagagaa aattcagcaa
    17941 atatttctat gataaaaagc ataagaacaa ggttttgaag acctgaactt atgctttttt
    18001 acgttcttaa agactgttta tactcaaaaa acagttgaac aacttcaacc acctcttata
    18061 agaactttat actattcgag aatctcttca aaccacgtca gctctatctg caacctcaaa
    18121 gctgtgcttt gagcaacctg cgactagctt cctagtttgc tctttgattt tcattgagta
    18181 ttaattctcc ttttccaact catacaaatc tgcgataata gctgcgacat gtttgatatc
    18241 ttccagcatg cctcgcattt caaagtcagc caatacaggg aagccaaagc gttgactgta
    18301 ttgcttggct gttaggcagt attggttatt aaagttacga tttcctgacc caaccacacc
    18361 aaaacactta ctagcattgt taccataggc aataaaatct cccaccggtg tcgtcaaaat
    18421 ctcaacatct ccgttatcca cgccattccc accttcgaga taggtcggca aaaaagcgac
    18481 atagggatgg tccatttcat agaaattttt gccttccttg accaaatcct tgatatgaat
    18541 cttttgaacc tcaatccctt tgtactggga caagagatag tctttcaagc gcgtcacaaa
    18601 actttcagtg ttgccactca aactaatata aactaaagaa attgtcttca tatgtacctc
    18661 catgatttta tctataatca aaaaacaact accaagattg tatcttgata gttctttttt
    18721 gtcaatactt aggcaagctc ttctgtttcc tcttcttctg ccaaggcttt ctgttgtcgc
    18781 cacatcttat agaagacaag ggctaggaag agaacattga tgacgatata gaaaatgcta
    18841 acagcttttg aaccaatact cactgtcaaa cgcatgctct catcttgaac caactgcaca
    18901 gcgtcttgta aaccaacata gccataaatc gaaccaataa tagctaacaa aacatagcca
    18961 acgtaagtat agtttgcata ttgcaaattt ttacgaacaa ggaagacaat cgctacgacc
    19021 actaaaatag tagatagcac aatcaaagca acattaaaaa tagaatgaga tgcttctgct
    19081 actctatagg tataattaag ctggtcttct aattgctgag cattgacccc tgcaacttgt
    19141 ttctgagcag cacgaagggc ttctttacta ggcaagggac tcacaattcc aaacagagac
    19201 ggaactgaaa taagggcaga caagattagc aagacccata gataaattgg tttctttttc
    19261 atgatagaac ctcctgataa tattatagca ccttttgtaa aggaatacaa attttatagt
    19321 atagtttcta cctgttttcg gtaggctttt ggcgattttc cgcacaattt tcggaaaact
    19381 ttatagaaat atccaaggtt actgtaacca acagcataac aaatatcttc gatgctgtca
    19441 ctagttgaaa gcaaaagttg ttgggcagcc ttaatgcgtt gtttgtttag taactctgca
    19501 aaagtcgaat cggtttcacg cttaatcaac tgccctagat agacaggatt gataaagaga
    19561 gccttactga tatccttgag ggaaagctct ttttggtaat cacgaccaat gacttccagc
    19621 acactgacca cattttcatt catacggtat tgaccgaaaa agctgctcag agtttccttg
    19681 atataaggaa ccaattcatc gaaggattga atagcatgaa tggttttgac gatgtctgtc
    19741 aggtcatcag ctttgagatg ttcaaataaa tggaaaacat ccatgacaaa ctgaataaaa
    19801 agctgtttag taatcggaac acgcggcgta ttttcaagaa ctaccttctc caagagattt
    19861 aactcttcta cgatttgatt gagatttccc tgaatgataa ccctataaat cggttcgtaa
    19921 taggcaaaga gactctcaga ctgctgcaga tttacagaac cgtaaaagag ggttttctca
    19981 gcattcagct tccagcgttc aaagtgttct tgataaggag cttcaaaagg tgtaacgact
    20041 aaaccatcta ggggttggtc agaaatgaaa atctgccagt cttgacccaa gacatagtag
    20101 ggaatggtga aggaaccttg tttttccttg gatagaccta tccaccaatt ctccttatcc
    20161 cctaaataac taacaaatcc agcctcgtct aattcttgac taagagtctg acttttcttc
    20221 cctctctcgc cgagctgacc tgcaatcttc tccagcagat ttcccagctc taccttatca
    20281 acaggcttga ccaaatagtc caccacacta aggttcattg ctctttttac ataatcaaac
    20341 tcctgataac ctgagagcag gatataggca gcatctggta agatctcttt catctcccga
    20401 atcatatcaa gccctgtttt gtctggcata ttgacatcgg aaatgatgac atcaacagga
    20461 ttttcctgaa catattctag agcttcatcg gcatgactgg ctgttgcgac gacctccata
    20521 tcccacttat caaagggaat caaacgcttc agaccttctg tcaccatgta ctcatcatct
    20581 actaataata ctttatacat tttctccctt tctactcatc ttgaattgta atacgatact
    20641 gaacaccggc ttgctctgca gactctatcg taatggcata gcggtctcca aaatagagca
    20701 caaaacgctc gtgtacattg acaatcccga tagactgcct ttgatcactg tagctggctt
    20761 ggtgttcaaa atatctctga cttaattttt ctcggatatt tgccaacttt tcagccgaca
    20821 ttcctctacc attatcgacc accaaaattt ccacaaaccc atcctgttta agagccttga
    20881 tgctaatcac attatctgtc cgcctgtggt caacaccatg cgcgaaatag ttttctacca
    20941 gcggttgcaa ggtaaacttg ggaatcttca tattctctaa ctctggatct atcttgaaac
    21001 cataggcaat ggacttggga tagcgaacca tgcagagata gctgtattta cggcaaaatt
    21061 ctaattcctg tttgaggagg gtctctcttt cgtcggaaat attgttacgc aagagactac
    21121 tgaattcata aatgatatct gccaactcat cttgactctg catgactgcg tacatacgca
    21181 agaactccag cgtattatac atgaagtgag ggttgatttg cgcctgcaag gcccgcatat
    21241 tggcatcttt ttgactgagt tctaactggt aaatatcatg gatattcttt tccaatcgat
    21301 ccaacatatc attggtcgtc tccgcgatta ggagcaattc ctgatccttt tcaagcgtat
    21361 caatgcgaag accttcttgt ccttgggcaa tagcttggat ggaatccacc aaatccacta
    21421 cctgcttttg ataattagca aaggtctgcc tcaaagtcag ataaagaatg acaataaaga
    21481 gaaggctcaa gcccacaatc aaagcagagc tggtcaccgt tccttgtaaa acaaagtttt
    21541 tgggaactgc cacctgaacc tgataaccat gagaagttaa gccaactagc caattttcac
    21601 tctccttatc aactgtctca ccaatatgaa aaatctccgt atcaaaaggt gaggtcactg
    21661 ttactgccat cggagtatga cctctagtat tatcaatggc atggtataaa acagcaggat
    21721 ccaaggagat gtaaatcact cctaagtctt gatctgacac tggatctgac accggaatgg
    21781 caaaactatt tcctgctggt ttgaaatcct cagccctgat tttttctcca ctacgtttgt
    21841 ctttagtaga aacatagact tccttgtaat cttgaaaagc aatggccact ccagttacaa
    21901 aatcatttcg aacatagagg tcatcaacat tttcatacag agagactgat atataaggcg
    21961 aagctttccg ctctaattgc cagtaaaaat agtctggcat gctaaggcta aagtatttat
    22021 agattccctc aatacgagct tggttttcaa ctaaagcctg agctatctgg gttgactctc
    22081 tatggtaata ttcaatttca ctcactgttc tagcagttac acgctgggct actctctggg
    22141 cttccttttc acgtgaatcc cagtcagcgt aagagagtaa gattgcaaaa ctcgcaatga
    22201 taatgatcat cactgaacta taggttctta gaagcgtatg tagccaaaag tgcttcattc
    22261 tattttgtcg ccaatttttc atggatgatt tcatagacag gttccaacat atcactgata
    22321 aagaaatgcc acatcccaat tcctacaaaa aatagcaggg caggcatata gtaaccaatt
    22381 gctacaagta gcacagtccc aaggagaacc ttggccacag ctgctagact cataaagatg
    22441 ccaatcaagg ataatttgag actatttcga taggacaagg caaaataaac ttgtaatttt
    22501 aaagatactg tataagccaa aaagatcagg gcaactacta ggacattcaa aagggtcgca
    22561 atcaaatgaa caatggtctg atgaggcaat tgcaccaata gatacaaacc ataggtcaaa
    22621 actagaccca cacctaaaaa gctatagaaa atcaggttgc ttgagacaaa attttgcttg
    22681 taaagagacc aagcctcctt caaactgtat tcccgaaagc tataaccatg ttctgcatat
    22741 aagctcatca aggtggcact agctggcgct agaccaagga taatccctcc tactaaagtt
    22801 aatagccaaa agaaggctgt cgcaatcatc cctaaaaaga aacgattaaa gacaaagtct
    22861 aaaaatctac ccatgatatt cccctaaaaa taactatgaa accattatat catatttcaa
    22921 gcgttttcaa aaacttctag tatccattta caatcccacc aaaccgtggt acaatgagaa
    22981 ttgtgaaaaa attatcagga gacaattcat gaagaaatct atcttaacta cactgctttt
    23041 tgcagttctt tacttcctct gcatggggat tggtgtcctt ttgggcaatc tctttgacca
    23101 aactggaaac atgttttatg cgcctgcctt tactgccctt gtcggcggta gcgtctatat
    23161 gatcctagtc gcaaaagttc cgcactttgg agccattacc actatcggcc ttgtcattgc
    23221 cctctttttc ttgggaacta aacacggtgc tggttccttc cttcctggaa ttatctgtgg
    23281 cctcctagca gatggagtag ctcatttagg aaaatacaag gacaaaacaa agaacttcct
    23341 ttctttcatt attttcgcct ttagtacaac aggaccaatc ttgcttatgt ggattgcgcc
    23401 caaagcctat atggctactc ttctagcaag aggaaaatcc caagaatata tcgaccgtat
    23461 catggtcgct ccaaaccctg gaactgtcct tctatttatc gcaagtattg tcatcggagc
    23521 cctagtgggt gccttgattg gacaagcctt gagtaaaaaa tttgcacaga aaatctgatc
    23581 agttaaaaag agccacgcgg ctctttttat ttatgactca atttcttagt caagaaatct
    23641 cccaagaatt ggattgcaaa gataatcaaa atgataataa tggttgccaa gatggtcaca
    23701 tcgtgattgt agcggttaaa tccataagcg atggctacgt taccgatacc accagctcca
    23761 accgcaccgg ccatagctgt ttccccaaca agggaaatca aggtcacagt cgtcacacgg
    23821 atcaaatctg gaagaccttc tgataggtaa acacccacga tgtcccagaa agtcgctccg
    23881 ctcgcttgag ccgcctcaat gacaccgcca tccaattcag ccaagacaac ctgcacctga
    23941 cgggcaaaga aggcaaagac tgcaaaagaa agtgggacaa gggctgcatt tggcccgata
    24001 cttgttttaa caatcaagtg agaaagtggt gacaagattg ccaagaggat gataaaggga
    24061 accgcacgaa aaattgaggt aattttgtct aaaatccaga atacgacttt attctccaag
    24121 acaccacctg gcgctgtcaa gacaaggaaa agacctgcca ctagccccaa gaagcctccg
    24181 ataatgaagg aaagaactgt catataaaga gttaagtaga tagccgttcc ccagcctgcc
    24241 tgaccagccc aacccatttt atagacattt ggtaaatagg tttgaatcaa tgattccatc
    24301 ttactgtact cccttcaata cttttagttg tacacctgct tgacgaatgg cttcttgggc
    24361 acctgccaac gctgcttttt cacctgacaa gaccaccacc aattctccaa caggagtacc
    24421 atcgagaatt tcgatattcc catagagaat attagccatt acttggtaat gcttgtacaa
    24481 ttcattcaaa agtggctcgt ctgttgaagc tccagcgtac ttgagttgca ccaagagact
    24541 gttttcagac aagtgttcca cgatttcttg cttctcgatt ttgaccatgg cttcgtcaat
    24601 acctgtggct gttgagataa agtcttgagt caaaggttgt ttagggtttg agaagatttc
    24661 aaggacactt ccctcttcaa tcaaatgccc atcctgcata accgccacac ggttggcaat
    24721 gtctttgaca atctgcattt catgcgtaat caagacaact gtcaatccta atttttggtt
    24781 caaatcttgc aacaaggcca aaatctgctt ggttgtctta gggtcaaggg cagaagttga
    24841 ctcgtctgaa atcaagattt ttggatcatt ggccaaggca cgcgcaattg ccacacgctg
    24901 tttttgccct ccagataatt gtgaagggta gttttcagca cgatctgcca aaccaaccaa
    24961 gtccaacaac ttagctactt tagccttctt ttcttccttg ctgagtccag agtgtttaag
    25021 ggcaaaggct acattctcct ctgctgtctt ttggctcatc aggttaaaat gctggaaaat
    25081 catcccgata tcttgacgtt tacgacgcaa ctgctctgcc gtcaaggtga ccttaccatc
    25141 aaaaatcaca tcgtcgtcaa tggtaatttt ccctgcagat ggtttttgca agaggttaat
    25201 cacccgtaca agggttgatt ttcctgctcc agaatatcca acgattccgt agatatcccc
    25261 ttcttggatg tgaatggtca catccttaac cgctgtaatg gttctcttct tttggtgaaa
    25321 agtcacatcg atctgatcta acttgataat atctctactc atagcttcta atcagctcct
    25381 ctactaattc gatatgggtg taataatcag cgattcgcac attttcatct ccaccgtggt
    25441 ctcggctatt ggcatttcct agaccgaatg caaccattgg tacctctagg gcatcaaaga
    25501 ccgtatgcat aggtcctgtc cccgctgtcg tcggcaagac tgaaacgccc tgtggataga
    25561 atttcttggc caactcgatc acattgagaa tggctggtgc gctcatatcg cttcgatagc
    25621 tcatctctcc caaggtatag tataattcta ccttatcaaa gccatttttg tctagttgtt
    25681 tccgaatttt ttccagaaca tcatgcggtt ctaggcccgg aaccagacga acctctagct
    25741 tggcactggc ttctgcaggt aaaatagtct taacaccctg accttgataa ccagactgga
    25801 ttccttcgat attaagcgct ggctcgaaaa agaaacgttt tagaaaggcc atccgctcct
    25861 cctgtaagag aggcaactcc aatccataaa tccgactaac ttcctctggg tttcgttgac
    25921 cataagtttc tagcaaggcc atttctcgtt cattgggctc ttgtacttct tcgtacaagc
    25981 cttcaaccaa gatacggcca tccgcagcac gaagagactg taaggcttgg aggagatacc
    26041 aaggagctga ttccacaaca ccaccataac tcgagtggat atccacatca gcgcttttta
    26101 ccttggcatc aaaggtcaca attcccttat tgccaccaga aatttccagc tgttccaagg
    26161 catttttggt cccttgttcc cagaccaaca aatccgcccc acggagtttg tctgcatgct
    26221 tttccaaata cttatctagg tctgttgaag ccgattcctc cgctccctcc atgataaagc
    26281 tgatattgac aggtaaatca tcatggtgct gcatatattt tctcaaagca ctcaagcgag
    26341 ctgtgatatg acccttgtcg tcatcaaccc cacgcccata catgaagcca ttgcggaccg
    26401 aaagcgtaaa aggatcctct gtccagacct gatccccatc cgctggcaca gtgtcatagt
    26461 ggttatagaa aatcaaggtc ttggcatctg gacgcgaact cttgaaatgt gccatgacaa
    26521 agggtgctgt atagctctca tcaatctcca cttcagctcc aacacgcttg aaaatctcac
    26581 ccagataatt tgcgacttcc ttgagtccaa cctgctgggc aaagactgat ttcttagaaa
    26641 tcaaggtacg caaaacctca aaataatgtt ggactacatg atccttttca aatttttcaa
    26701 tctgttcttg ttcgctagga aaaaccatat tctctctacc tttctatgaa cgaatagtct
    26761 tctttacaat ctatactcta tacaaaagca agaggtggaa ttttctctcc cacctccctg
    26821 tttcttatta ccaaactggt tgatccaaac catctgatga ttcttcgata acttttttca
    26881 cgtcatctgt gtggtaagct gcgattactt tcttgatagc atcagcctta ggtgatgttt
    26941 cccaatcttt ttttgcaaca atgatgttgt accattgttt tgagttttca tcagcttgtt
    27001 ctttgaaaag tgctttcttg tagtccaatt ttgcttctgt aacgaaggta ttgtttacaa
    27061 cggcagcgtc aactgatgac aatgaacgag ctgtttggct agcgtccaat tcagtgattt
    27121 tcaagttctt tggattttct ttgatgttgg caactgttgc aagagcagtt ccagaaacat
    27181 ccaatttaat caagccagct gattgaagca aataaagcgc acggctttcg tttgtagcgt
    27241 cattcggtac agcgatttct ccgtttgctg ggatgtcttc tactttagtg tacttgttgg
    27301 cacttccatt caaacctgaa taaaggcgga ttggagagat gtaagtatct gcaatcgcta
    27361 caagatcttt tccgttttct ttgttccagt tattcaagaa gttatagtgt tggaaagcgt
    27421 tcaaatctac ttcgccatca gcagttgctt tgtttggttg tgagtagtct gtgaactctg
    27481 taaattccaa ggtaattccg tcttttttaa ccaattcttg gattttgtcc caacgttttt
    27541 cttcagaacc gctacggtta acagttgcga ttttgatagt tgttgcattg tctgctttct
    27601 tttctgagtt tccgcaagct gcaagagcca aacctgcgac tgtagcaagg gctgctaggc
    27661 caagccattt tttgattttc atgattcttt ctccttaaaa ataataccgt actagtatct
    27721 cacaatttgt ttgatttcac aaattgttat tagatagtca gatatatagt ttaaaactat
    27781 atccctaaaa actgagaaac cacccacctt actcagaatg gatgatttca agaaattatt
    27841 taatatcagc ttctgccggt aggtaagtgt ctccgaagaa ttgtttagac aatttttcaa
    27901 gagttccatc tttataaagg tctttgatgc gtttgtctac aaatgatttc aactcatctt
    27961 gaccttgagc aagaagtggg taaacgtacg gttgttggtc gcttggaagt tcgataactt
    28021 tcaagttgtc caaaccttgg ttcttgatca ctgtttcaac accgatttta tcaaaaatct
    28081 tatagtcaaa ttgtccatcg ctcaaacgta ccatgatttg ttggaaatct gccttagtat
    28141 agttaaggat agttgggttg tccgtgtgtt cagcgttgta tgcttctaac tgcttagctg
    28201 atgtagtggc ttgaacgact tccgtcgatt ttccaccgat atcatcgaga gacttgatac
    28261 tagagtcatc tttcttcacg acaaggacat taggattttg ggcaattggt gcggcataga
    28321 ggtatttctc cgcacgttct ttagtgtagc taagattgtt gacagccata ttgtaacgat
    28381 cagcgtcaag accagcaaag acacctgacc attctgtctt ttcaaacttg acatcatatt
    28441 tgtcagaatc tttaaagata gcgcgaacga cttcaatctc gtaaccagtc aattcgccat
    28501 tttcttcata gataaatggc cttggtgatc cattggttgc aacgatgatt tctttcttgc
    28561 tagctgcttc tccttctttc ttagcacccc ctgagcaagc cgcaagcaca cctgcagcaa
    28621 caagtcctag ggcagcaaga gatgagtatt taacgatttt tttcatgtca tttcctccaa
    28681 aatagaatac cttataatct taacagaaaa agaccattta cgccattata tgatatctat
    28741 ctctgtgata agttttcttt atagttgatt taaaaggcca aacgcaagat ggcaatcaag
    28801 actactccaa agagaactgt tccgactaga ttgcgatagc gaaaggccac ccaagctgtt
    28861 ggaaagacgg ctaagaagtc cagccatttg atttgaggaa gactgccaac cttgcctgtc
    28921 actacgcttg aaagaatcaa ggcaaagata atggaaacgg gcaagaactt caaaaaacgc
    28981 tcaacgattg caggcaagcc cttatacttg actaagatga agggaatcat acggggaatc
    29041 caagtcacca agccagagaa aataactgct aataaaagat acttactgac catctaaaac
    29101 cacccccatg ctacaaccaa gtagcgtcgc aaacagaata gctagtgact gagacaccac
    29161 tgtcaagagc aaaaagaagg acaccgcaac aactgctagg ataatgagca gattgcggac
    29221 aggaatccgt ctttgcataa tctgaaattg cgaagcaaaa ataccaataa acatcccaac
    29281 cagggcaaaa tccaagccaa agatttctgg atttggtagc aggccaccca gagccgttcc
    29341 gactactgtc cccacaaacc aagccacata gctgttaaga ttgtttccgt gcatccacat
    29401 aggatttacc ttgtctgtat gggccaattc acccatcaaa acgccatagg tctcatctgt
    29461 caagatacta gacataccga tattgtacca aagactggta tgacggaaat aggtcgacgc
    29521 atgtaagctc agcaaaaaga gacgcaaatt aatcaaaaag accgtcatag caatagctgc
    29581 cacaggcgct ttaaccgcaa tcagtgccag catggcaaac tgggcactcc cagcataaac
    29641 aaagagactc atcaagccca tctcaacaag tgtcacatag ggcgcaccga taattccaca
    29701 ggccaggccg atactgacat agccaagagc cgttggcatg gctgcctgcg ccccctccta
    29761 aaatcctttt tctttcatct ttctcctcat attgtcttaa taatactcaa tgaaaatcaa
    29821 agagcaaact aggaaactag ccgcaggttg ctcaaaacac tgttttgagg ttgtggatag
    29881 aactgacgaa gtcagtaacc atacctacgg caaagtgaag ctgacgtggt ttgaagagag
    29941 tttcgaagag tacaagtagg ctgaaaagaa tccagccaca gcatggacta ttatatagca
    30001 gattgaaata agatgagaac aaatcgattg ggaaagtaaa attaatttct ataaatgttt
    30061 tagcaattgt ttcgtactat tttagattca gtctactata acacattcag aaaagagaaa
    30121 aaagtctgtt gattttgacc atcataaaaa gactggcaat ccagtctcaa acatatatta
    30181 tagaaattct ccactaaata ctttcacgaa tattcagaag catgacaaag gcaactagaa
    30241 gaaatagtaa taaaacaaag ctaactgcca gagttccaaa gctagtagca atggttacca
    30301 aagctattgt aaataagcta ggtaaaacaa ccgtaatggc accgatagaa gattgaactg
    30361 ctcccattga ctcctcaggt atttgtttaa agacgagttc ttgcaatcta ggagagagaa
    30421 tacccgcgaa aaaggcatcc aaaaagctaa agatcagaat caagccaaaa tgagctgtcg
    30481 caaagcccac taggagtagt aattggagta ggagtgaagc atacagggct atttttatgg
    30541 aaatggtatg ttgcagatag ccactcatca gacttcccaa aataagagct gacaattcta
    30601 gtgtggctaa caaggcaaga gattgacctg tttgtaaatt caaaaagggc tggttcctta
    30661 aaaatagagt ggaaatagga accgtaacat ttatcactgc ttgactagta gagataataa
    30721 acaaaaccaa gagcacctta ttcatattcc atatcaattt cgatgattgg agcaaatgct
    30781 ggcaaaagga ttttacaaag agtccttctt gatagctaat cgttttttct actttcaaga
    30841 ggtcagtttt tatgaagagg atacctaaaa atgcgattaa aaaggtaaga gcgttcagta
    30901 aggaaataaa ctggatggat agaatgccta gtaagactcc tcctaggata ttactgattg
    30961 ttttcactaa actaacagtt gactgtttaa agccaatagc ttctgccaga tggtcttgcc
    31021 caataattct aatgaaaatc ggagtgagca tggcgcctga aaaataactc aatgtgtcag
    31081 acaagaggtt aatcagacaa ataaatgcta ctagcaacaa ggagaaagac tgccctgaaa
    31141 gtgataaaga cactatagag taaagcaaaa attttgcaaa actaatgact gtgtatttca
    31201 agacacgatg atgttgaaaa tccgccaaaa ctcccagaaa gatttgtaga acttggggca
    31261 gggtttctga aatcgtgatg agtaaaatcg ccaaaggggc aaaagatgca tctgccacat
    31321 aattcaggaa ggccagataa aaaatcgtat ccccaagcgt tgaaatccac tggttgatgg
    31381 ttaattgtct aaaatctcta ttttgaagaa atactttcat cacaactcct tcttaaattc
    31441 aaatgggaag ctttccccaa ggatagaccg cgatactact aacaaccaaa attacagtaa
    31501 catcaaaagc tgaccaatac cattgtagac tatatgcagt ccaataggcc aataaattga
    31561 ctttgtcatt ctaaataaga ctgcaaatat aagacctcca cccatataga agacaaagtc
    31621 tgtcaagacc caaccgtgat tactaatgtg cgagacccca aataaaacag cggaaccaag
    31681 tacatctagc ccccatttct ttcctttttc cagagcagtc atcactaatc cacgataaat
    31741 catgtcttca aaaatgggac ctgcaatcac aggataaaaa aaatacatca aaaatgccgt
    31801 agctcctgta aaagtaggag cagcatgttg ataagaaatt tcatttcgag taggtgggaa
    31861 aagaaaaaag gtgacaaaat tccaagcaac aaaagcaagc agagctagga aggaatagaa
    31921 aagataggat cctttaaact ttctactatt gattttctgc catttccccg accaaatcat
    31981 agcaataaga gcaaataaaa ccacaagaaa attcaacatc atatccgaca gataataggc
    32041 aaagtcagat agcccagcaa caaggtcgct gcgtaaaact aaaacactga acttctggtc
    32101 agcaataact agtagaaaaa ctataataaa gtagcggtgt gagattatct ttttcattct
    32161 atttcctcct atacatatga aataaagaca gcccctaccc aagaaaaggc aagggctttg
    32221 gatattaata acgacggcgg tcagaatcaa agtcaggtct atcgttaaaa tttttcttcc
    32281 agaaattagt gattgaccaa ggtgtcccga accctcccca agcaccggct gggataacag
    32341 cattattccc acattcccct ccataaactg tctccaactc agcttctgtc aattccattg
    32401 tttctgcaaa ttgtaaattt aacatctttt acactccttc aattatcttc atttgtaaac
    32461 cacttctgcg acctaggatt tgcttcaagt gctttacaag tacagtataa cacgaaaatt
    32521 ataacacgaa aattggctta ttttagaaaa tcgcatattt gatatttttt cttatagaaa
    32581 tttcagattt gcgattttgg tagatttgat tatttccctg gtataataaa gttattacta
    32641 atgaggaggg aagtaatatg aagaaacaag ttttaacatt attgacgatc gttgctgata
    32701 ttattatctt tttcccattt ctaactaatc gataagctct ttgtattgct gcaaacgcaa
    32761 ttcaaaaagg gctgtcaatt gtggattttc taatacttgc agagattgga taaagtgttc
    32821 aatctctttt tgattgcttc ctttggtttg aagaaaaatc tgcattttct ttaaaaactg
    32881 ccacaatact ttttcaaaaa catcgtatgg acggagcatg cgctccagtt cagcttcaaa
    32941 gattggaata taagaaaata gcttgcgctc cataagttct gagatgatat taagataacc
    33001 acttttcata taccatcgat tgtgtactaa ctctttaaat tccttggatt tttcgagtaa
    33061 ggaggttgat aaaaagatca gatcttgatt gctcaagaag ggcatagtat tgcaaaaaag
    33121 atagagttca aaccaggtcc aagactcaat agcgtagagg taggtagtca aaaactcgct
    33181 atcctcctct gttagcgggt agcttttatc taaggaatgg agtgcatttt taatcacaat
    33241 ggcattcaaa cgacgatagg tctgcgccat ctgttcttga tcgacttcct ccaatagctg
    33301 ctctaaagca gctatatcct gatgggcaaa gcgatccaca acctttcgac cgattcgcat
    33361 atgtggagat tcttgatagt tgttgagctt gtgcccaaac tcatcaaaat tcatattgat
    33421 cccttggatg gctaatatga gcttatctgc agatagcata gactgtccta gttcaaactt
    33481 ggacaactga gaagctgtta gaccctcaca ggccacatct gactgcttaa gttttctcgc
    33541 caagcgcaat tccttgtaaa attctcccaa ttccattctc tcaatcatct gaccacctcc
    33601 tagcttttgc atattatatc attatttaca atcatttgtc aaaatattct gatatttaag
    33661 agggcaaaaa agccagcctt aagctgactc taaaaggacc gagccttgta caatacagaa
    33721 ctcaatcctc gttctaaata gaatgtctaa cttttttggg cagtacacga gcttggggaa
    33781 cagccactgg aacgatgagg tgtgagctca aaatatcctc cagttatgtt tttcctaata
    33841 gtataccgga agagtgaaag gattttataa tggagcggtt acaaagaacc tactttctat
    33901 taaacagtat actatgaaaa tgtgaaaatt taacattttt ttgtacaaat tttataaatt
    33961 attgcctttt taatatcaat agttaatctc ttatccagat cccccttgtg taaactttat
    34021 ctttataagc ttcaaggccc ctatcccatc tatttacaac aa
//
LOCUS       DAVLVZ010000019        31356 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000019 DAVLVZ010000000
VERSION     DAVLVZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31356
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.019"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(1..1036)
                     /locus_tag="VYN77_000854"
                     /pseudo
     CDS             complement(1..1036)
                     /locus_tag="VYN77_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1396..3366)
                     /gene="tkt"
                     /locus_tag="VYN77_000855"
     CDS             complement(1396..3366)
                     /gene="tkt"
                     /locus_tag="VYN77_000855"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1558335.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPIVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            complement(3403..4098)
                     /locus_tag="VYN77_000856"
     CDS             complement(3403..4098)
                     /locus_tag="VYN77_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1558336.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            complement(4109..5194)
                     /locus_tag="VYN77_000857"
     CDS             complement(4109..5194)
                     /locus_tag="VYN77_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEV1558337.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            complement(5220..5531)
                     /locus_tag="VYN77_000858"
     CDS             complement(5220..5531)
                     /locus_tag="VYN77_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEV1558338.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            complement(5559..6008)
                     /locus_tag="VYN77_000859"
     CDS             complement(5559..6008)
                     /locus_tag="VYN77_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1558339.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            complement(5986..6414)
                     /locus_tag="VYN77_000860"
     CDS             complement(5986..6414)
                     /locus_tag="VYN77_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1558340.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKPPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSGIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            complement(6407..7870)
                     /locus_tag="VYN77_000861"
     CDS             complement(6407..7870)
                     /locus_tag="VYN77_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1558341.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKG
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFKMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFSELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSAFLTEGDKQRLQAKIQEINYE"
     gene            complement(8399..10735)
                     /locus_tag="VYN77_000862"
     CDS             complement(8399..10735)
                     /locus_tag="VYN77_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV1558342.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAHKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLILLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            10868..11617
                     /locus_tag="VYN77_000863"
     CDS             10868..11617
                     /locus_tag="VYN77_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1558343.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            complement(11909..12523)
                     /locus_tag="VYN77_000864"
     CDS             complement(11909..12523)
                     /locus_tag="VYN77_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1558344.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(13338..13607)
                     /gene="rpsO"
                     /locus_tag="VYN77_000865"
     CDS             complement(13338..13607)
                     /gene="rpsO"
                     /locus_tag="VYN77_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1558345.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(13886..14377)
                     /locus_tag="VYN77_000866"
     CDS             complement(13886..14377)
                     /locus_tag="VYN77_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV1558346.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVIGGAGS
                     LYIDETKMTRLLDTPDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGN"
     gene            15235..15456
                     /locus_tag="VYN77_000867"
     CDS             15235..15456
                     /locus_tag="VYN77_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1558347.1"
                     /translation="MELQELVERSWAIRQAYNELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIW"
     gene            15616..17559
                     /gene="thrS"
                     /locus_tag="VYN77_000868"
     CDS             15616..17559
                     /gene="thrS"
                     /locus_tag="VYN77_000868"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1558348.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(17608..18582)
                     /locus_tag="VYN77_000869"
     CDS             complement(17608..18582)
                     /locus_tag="VYN77_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1558349.1"
                     /translation="MLDWKQFFLAYLRSRSRLFVYLLSLAFLVLLFQFLFASLGIYFL
                     YFFLLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(18575..19252)
                     /locus_tag="VYN77_000870"
     CDS             complement(18575..19252)
                     /locus_tag="VYN77_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1558350.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSIFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            19408..20481
                     /locus_tag="VYN77_000871"
     CDS             19408..20481
                     /locus_tag="VYN77_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1558351.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYHAWKNR"
     gene            complement(20572..20874)
                     /locus_tag="VYN77_000872"
     CDS             complement(20572..20874)
                     /locus_tag="VYN77_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558352.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(20943..21380)
                     /locus_tag="VYN77_000873"
     CDS             complement(20943..21380)
                     /locus_tag="VYN77_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1558353.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(21456..22298)
                     /locus_tag="VYN77_000874"
     CDS             complement(21456..22298)
                     /locus_tag="VYN77_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1558354.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            22410..23060
                     /locus_tag="VYN77_000875"
     CDS             22410..23060
                     /locus_tag="VYN77_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1558355.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLTLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            23241..23888
                     /locus_tag="VYN77_000876"
     CDS             23241..23888
                     /locus_tag="VYN77_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV1558356.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            24019..24432
                     /locus_tag="VYN77_000877"
     CDS             24019..24432
                     /locus_tag="VYN77_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558357.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            24451..24636
                     /locus_tag="VYN77_000878"
     CDS             24451..24636
                     /locus_tag="VYN77_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558358.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(24903..25346)
                     /locus_tag="VYN77_000879"
     CDS             complement(24903..25346)
                     /locus_tag="VYN77_000879"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1558359.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(25376..27598)
                     /locus_tag="VYN77_000880"
     CDS             complement(25376..27598)
                     /locus_tag="VYN77_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1558360.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            28594..29223
                     /locus_tag="VYN77_000881"
     CDS             28594..29223
                     /locus_tag="VYN77_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1558361.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSYWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(29346..31238)
                     /gene="pepO"
                     /locus_tag="VYN77_000882"
     CDS             complement(29346..31238)
                     /gene="pepO"
                     /locus_tag="VYN77_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1558362.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         9312 a         7069 c         5715 g         9260 t
ORIGIN      
        1 tattctcgta gtgcttctct ttgaatgcct gttccaacat tgtttgtact tgttctaaat
       61 taggcgaaca agaaagatta taggcgataa tttcgctgtt aaagccatct aaaactggtg
      121 ataagtaaag cttttgagta cttgctggaa tggcaaattc agtcacatct gtgtagcact
      181 gttccattgt tttagagcct tcaaattggc cttgaatgag attctctgcc ttcttaccaa
      241 cgtctccttt attttcgttt ctgtcgcatt ttagcttgta aattgagtac tttcatcaag
      301 ccttgaactc ttttatgatt taccagataa ccacgatttc ttagttctaa atgaatacag
      361 cgataagcat aatttccctt gtgttcgata aaaatggatt gaatttcagc tttaagctct
      421 tggtccttat ctggtttgtc tagctgtttc aagtgatagt agtaggtcga acgagctagt
      481 ttaatggctt ttagaagaag atctgacgaa aactcagtca ttaattcttg aacaatttct
      541 gtctttcttc tttctctttt tcctccttca atcggagttc tcttaacttt tttaggatgg
      601 cattctccgc tctcaggtac tcattttctg cttgaagatg ttctaattct gtcctctctt
      661 caggtctcgt ttttggctta cgtcccattt taggtactct ctctcttgtt ttctcaacaa
      721 tagtataccc gtttttcctg tattgtgcta gccagttaag aagtatcgta cgacttggga
      781 gaccgtattc aagagaaact ctatctttag tccagccttc atgtcagact ttattaatca
      841 tttcttgttt taaatcagga gaatagtaac gattttttcc ttttttgacg aactctattc
      901 cgtaacgatc aatcaattta atcatgtacc taatattaga attgtttatc ccaaatttat
      961 ttgaaaactt ctctaagcta tatccttgtt ttctaagttc atagatctga actttatcat
     1021 cataagttaa cttcatgata aaaacacccc aaaagttaga ttttttctgt ctaacttttg
     1081 gggggcagtt catcatttca acatcctata ctatgcgttt tttaatttta gagaccttct
     1141 ctctagcttt caaaaaactt acaatatcct aagtcaatct gtggatgctg tgtttattga
     1201 ttaaagaaac tatctagatc agtagcaaag tgattagcgt acttcgcaac cgtttcaggg
     1261 tatactatct actataactt tcgtagataa attattacat tctcattata gtttttaaat
     1321 aaccaatcat ctgattagtg tctatcacta catctgctag tctgacaaca gctgtatctt
     1381 cattgtaatt ggtttctata gggacttaac ttgagcaacg atattctcta ccgtaaatcc
     1441 ataattatca atcacagtct gagctggggc agacgcaccg aagatgtcaa taccgatgac
     1501 cgcgccatcc aaaccaacat acttgtacca actttgggtc gctgccattt caatggccac
     1561 acgacgacga gtcttagatg gtaaaatgtc ttccttgtag gtagcatctt gagcatcaaa
     1621 tagttcggtt gagggcatag ataccacacg tactttacca ccttgtaaaa ccaattcctt
     1681 agcagctttg atagctagat tgacctcaga tcctgtagca atgataatag tatcaaatcc
     1741 cggggtatca tacacgacgt aggctccttt agcgacctta ccaaagtctg tcccttcttc
     1801 aactaccaag ttttgacggg ttaagacaat gacaattgga gtggtggtac tggtcaaggc
     1861 atgatgccaa gccgcttgag tttcacgggc atccgctgga cggataacag tcaagtttgg
     1921 cattgagcgt aaacctgcca aatgttcaac tggttcatga gttggaccat cttcaccaac
     1981 ggcaattgaa tcatgggtaa agacataagt tacaggcaac tcctgaatgg ctgatagccg
     2041 aatagcagct ttgacgtagt cagagaaaac aaagaaggtt ccgccataaa ctcgtaaacc
     2101 accatgaaga gccattccat tgaggattgt tcccatggca aattcacgta ccccaaactg
     2161 aatattgcgg tttaatggat tatatttatc ttgtaagcca tctgccttga tgtaggtcat
     2221 gttggagtga gctaagtctg ccgatccacc taagaaggtt ggtaaaactg ctgctgctgt
     2281 attaatagca tcttgggacg aattacgagt tgcttgagag aagccattct catagacagg
     2341 gaagtctttt tcagtaatgg ttacagggga ttttccagct acaatagcgt caatctcact
     2401 agcaacttcg ggataagcaa ccttgtaatc agacaccaaa ctagcccaag catcgtatgc
     2461 ctcctgacca cgatccgcta catttgtctt gaaatcagaa tatacttcct ctggtacttc
     2521 aaatggatcg taatcccatc ccaaaaactt acgagttgct cctgtttctt ctgctcctag
     2581 tggtgcacca tgaacagcat ttgtaccact tttattgggt gagccgtaac caattaccgt
     2641 tttcacttca atcaaactcg gcttaccaga aaacttggcc gtctcaattg ctgtagaaat
     2701 tgctgctaaa tctgttccat cttctaccag aactgtatgc caaccataag catcgtaacg
     2761 ggcgcgaaca ttttcagaga aagtatcttt ggtctcacca tccaagcaga tgtcgttgga
     2821 gtcgtagagg acgataagct tatctaaagc ttgatgacct gcataagaag ccgcctccgc
     2881 agacactcct tccatgaagt caccgtctcc agcgataaca taagtataat ggtcaaaaat
     2941 agggaaacca tctttgttgt acttagcagc taaaaaacgc tctgcttggg cgaaaccaac
     3001 ggcagtagaa atcccctgac caagcggacc agatgtcgca tccacaccag acgtatgcgt
     3061 cacttcagga tgaccaggtg tcttagatcc ccattgccgg aaatttttaa tctcgtccat
     3121 ggatacatcg ttataccctg ttaaatgcaa gagagcatac agtagcattg atccatgacc
     3181 cgcagacaag ataaagcgat ctcggttaat ccagtttggc tgctcaggtg taattctaag
     3241 gtgctttgta aataggctat aagccattgg cgcagcaccc atgacgattc ccgggtgacc
     3301 agaattagat ttgttaatag catctacgcc aagaaaacga atggcgttaa ctgattgtag
     3361 tgacatatat tcctcctaac taataaattt cttctcaaac cattatttga ttggcattgt
     3421 ttttccggtc atttcttcgt aatctctaga acagatatcc caggctttgg caatatcgtc
     3481 atccaaacca aataatccac tgcgacctat aacataaata tctggtcctg ccacatcaat
     3541 ttgtttgaaa gtcttacgac tcgaagaacc atccatctca atgatgtagt gataaccatt
     3601 ctgaactcta agctgacgga gttcttggat tttatacaag gtagactcca aaaagcgttg
     3661 tcctgcaaaa cctggatcta cagtcataat agttactttg tcaagtaaat caatgtaggg
     3721 aaagattgta gaaacaggtg tttcaggatt aaggacaaca ccagccttta gacctgcatc
     3781 atgaatttta tcaatcaaac gaaaagcaag accattcaga acttcagcat gaatacaaat
     3841 atactcacat tgtaaatcga gaacttgatc tacccaaaag gttgggtctg tgaccatcag
     3901 atgaactgat aaaggtgtgt cactaatttt ttgaacttct tgaatgaacc aaggagacaa
     3961 ggtaatattg ggaacaaaat ggccatccat aatatcgata tgataagatg ctactttatc
     4021 attcaaaaaa gtaatctgct ctttgaattt gtccaaatcc atggtcatca aagatggtga
     4081 aaattcaatt cttgacatcg ttaccctctt acaaaatttc aatatctgac aaatcaatct
     4141 cttcttcaac agtcataact ggttctgtat gttttattgg ttttttcaaa atcgcgtaga
     4201 caagtcctgt gactacaatg ttggctaaca aagcacaaat accagctact ggacgagtca
     4261 tggttggtaa cataagcact ccaccaaatg gcacggcaga atcagcaccc attgtcaaag
     4321 aaacagcacc accaacagca ccaccgatac ctgttgcaat gagacctgga accaagttat
     4381 tcataacaat cggaattaca ccttcaacaa tattgacaat ccccatagga acagccgatt
     4441 tcaatgtttc gatttcctct tgagtataga tatttttttt gagtaatttc gcgataaaat
     4501 aggccaatcc aaatccaact ggtgtagcag tattcaccaa ttgcaaagca gtcaatggtt
     4561 ctttcacacc ttcagcctgt aaagtcaaca caaaggcata gactgtttta ttaagtgggc
     4621 caccaaagtc aacagcactg agaactccaa taactgcccc catcaaacca tttgaagcac
     4681 ttcccaagct ttgtaataag ctcgtcaacc agttggtaaa ggctgcgata ggcgctccaa
     4741 taatataaat cataatcaaa ctacttacca aagaggctac aaaaggaata atcaaggttg
     4801 gcattaaacc tttaatccag tttggtactt tgaccttttt aataatcgct tgaaccaaga
     4861 aaccagctat ataacctccc aagataccac cgataaaccc tgaaccaaca gaattggcaa
     4921 ttagaccaac aacaaaacct ggtgcaatcc ctggcttacc agcaatcgag taagacaaac
     4981 ctgtagcaat aacaactggc aagagaccaa gggctttacc acccatagtt gctaaagcat
     5041 cccagatagt gaattttcct gctacaagag cgtcaggaac accacccccc attgctaaac
     5101 caatggcaac taagaatcct gcaccacaaa caattggaat catataggaa atggctgtca
     5161 ataagtgacc ttttaagttt aagtgtttca acatatattt tctccttaaa tattttcagt
     5221 tatttcgtaa caatctcaac agctttagca atcagtttat tgggagattt gactgccact
     5281 tctgttggaa ccttgataat ctttttaccc tcaaagcgtt ccataccaga aatcttaaca
     5341 tcaactgcta aaataactac atccgctgca tcaatctgct cttgactcaa ttcattttct
     5401 acccctattg tcccctgagt ctcaacatga atcacatgtc cagctacctt tgcggcattc
     5461 tctaattttt cctgtgcaat ataagtgtgg gcaattccca cagtacaagc tgcaacacca
     5521 acaattttca tacggatacc ctccaaaatt ttttcttatt aacaaaaagc tgcaatcaca
     5581 tcatcagatg tctgagcccg aactaatttg gcaacaactt cgtcattacc aagttttcga
     5641 gcaaagagtg ataaggtctt caaatgctcc ctagcagctt ctgtatcatc accaactgca
     5701 aagagtacaa ttactttgac ccctttccca tcaatggtct cccaaggaat ctcattgtga
     5761 tttatagcta tgactacccc cgccttctcc acagcagaac tcttgctatg gggaatagca
     5821 atataattcc caataccggt ctgtccttct gcctctctct gataaagacc ttcgataaat
     5881 tggtctctat cagacacata acccgtctca accaatagtt gagctaatgc ctcaaaaacc
     5941 tcttctttgc tctgcatctg taaatccgtc tggatcagac tcacattaag aatatctttg
     6001 atttccatat attatctccc gtaattcttc ttttgttaac tgttttaatt gatttatgaa
     6061 tgattcatct gctagtcttc tcatcaatgt tttaatacct gacttgtcct gtgatactgc
     6121 aatggccaaa ccaataataa ggtcaacaca ctggatatcc ttcgaccatt ctctgatagg
     6181 tggttttaat ctagtaatca ctaagacatg atgttgaaag tttccttcac aatgtggtag
     6241 aagaacacct ttagcaacct ctatacttcc ctgtctctca cggtaatata gaagctcttc
     6301 tattttttct gtatcttcag aaacaagaag gctgatttga tttgctaatt ctttgtaggc
     6361 ttcttgacga ttttgaacag atatatccat aaggacaagc gaaagattat tcatagttta
     6421 tctcctgaat ttttgcttga agacgttgtt tatcaccctc ggttagaaaa gcactaacta
     6481 ggacaaacgg gacacttgct ggttcctgca aagctaccgt cgtcacaatg aaatctaaat
     6541 ctggatatag atttatcagc tcatctaatt gatgataagc aactacatca ataatatcca
     6601 attcagaaaa ttgcttttct aatcgtgctc gtaaaagctc tgaagttccg acacctgatg
     6661 tacacatcac tactgttttt agaggacgtg ctcgcttttc ttgaaaccgt gcaaaatata
     6721 agactagaaa accaatctcg tccagactca aggacgcaaa accaaatact agactcactt
     6781 ctttagaaat agttgttaac tctttaacca gattaggata ggttaaaaga atttgtgata
     6841 aaagactatt cttaatctgt actctattat ctaatctcct cagtaaggga ctgatatgac
     6901 tcgccaagtc aacaaagtca ggatctgtcg tttcaatctc tctattgtcc atacacatac
     6961 ggctaaaata gtaatgagtg acatctaaaa ctcgctgaga aaaaggaagc ttcccggaag
     7021 aaaatggttt ttgcaatctc gaagatacaa cgtattgata aagatagtca atctcaactg
     7081 catcaacctt catcttaaaa tattgttcaa tttcttgaat aattttttga cagacactgt
     7141 aaattttctc atcaacaata gtaggttttg aaggtgctac aacatgaata ctagtactac
     7201 gacgattcct actgataaaa atatacaggt gagagaaaat atttatatta tatggatagg
     7261 gaatgtttac ccccaattct ctctcaagtg taatcagtaa tcccaaacaa aagtgagcaa
     7321 gttctccgtc aagaggctga accttattct gtgtaatttg ctctaaatcc aactgattaa
     7381 acattggcag tagatttaga atggctgaac gaatttgagc ctcatcccca tcaataaaaa
     7441 gctttcgttg tctcattttt aaatctaacc cataaattgc tagactctct tgtaatatct
     7501 gacgatcttt tagtactact gactcgctta cgtagaattc ctctcctaaa tcataaatag
     7561 aatgtggctt aggagcagtc aacaagagac gttctagtaa tttttcacgc cttacttcag
     7621 gatcaaaagc ctctgtaaaa ttcccatcaa cgtccacgag ggaacttctt ggatttaatt
     7681 tgaagcctct gcctttttca gataaaatga atgcttctac cggacaatct ttgttgattc
     7741 ccttgacaag gcgataaacc gttttatcag agcaatttaa tttttctccc aatactttag
     7801 cagatatata atccttacta tctcgcaaaa ggagcaacaa cgtttccatt tgttcttgtt
     7861 ttctagacac gatacacctc ctttatgatt ccattatatc gctttcaaaa aataatatac
     7921 ttcgcatttt gtcctttgca atggacattt ttgtttttat caaaaatatg atatcaaata
     7981 gaacagaacc tcactatgcc aaaatcattt taccttttct tcataacatc tattttagtt
     8041 gaaatattta agttgctttt cgtatttgat aagaaataaa ccaaacgatt ttctaataaa
     8101 taacactatg ttccatttaa caatagatag catcttcatt ttcaaactac caacctatcc
     8161 gaccatttgt tctagcaata gaagagagtt aaaattactt caatctgtcg ctttctcttt
     8221 cttacatcaa acttcaaata gtctaactac agatatttaa atctattacc tcaatccaag
     8281 caggcttgag aatcactctt gggttcaccg atagataata gacatgtagg ccttgcacat
     8341 cagatctacg atagatccat cgtataaaaa actgcaatcc acagattgca gtctttcttt
     8401 attttctagc ttgatagcga ctagtcagga tgaaaatcac tgtaaagacc aacatcatga
     8461 caccataaac aggcaacgtt tgttctgtca aggttgaaat ttcaagcagt ttttggattc
     8521 ttgggaagag agctgtggct aggaagcctc ctactgacca gacaatcaag aggactcgcc
     8581 atagagtgaa tggcatgcag gctctaaata cggataagaa accaattgac cccaagagat
     8641 aatagaggag agttgagatt tctaactcag accaaccttg actcgttcca aagattttca
     8701 caaaaagaac gctaaacacg accatgaggg cacttggtag ggcacgaagc atggattttc
     8761 tgaggaaatt tggctcaaca ggtttgatat ttcgctcaaa agtcagaacg aatggtggga
     8821 aaccttccac aaactggtca atcatggtaa tctggattgg aatgaagggg aaaatcaaga
     8881 tccattcaga ccgacctagt aaagcactgg cgatacagat aactgcgagc aagaatgagt
     8941 agatggtctt tatcaagaaa atcggggcga tgtgggcaat gttattgacc acgcgacgac
     9001 cctcgaagag aatctcagga acatcattaa agtctgagtt caagagaatc agattggcaa
     9061 tctgacgggt tgctggatcc ccctccgcca tcacgataga acaatccgcc tcacgaaggg
     9121 ccaagatatc attaaccccg tcccctgtca tagccgttgt atgacccgct tttttcaacg
     9181 tttggatgat gagtttcttt tgatgagggg aaacacgtcc gaaaatagct gtctcctccg
     9241 ccatggccat caattcctca tcggtgattt ttgagcaatc tacatagctg tgatagtccg
     9301 caaaaccagc cttatgggca atgctggaca ccgtaactgg attgtcacca gagataatct
     9361 tgagtcccac ctcctgagaa cggagatagt ccagcgtctc tgctgctccc tctcgaatgg
     9421 ggtccaagat ttccagcaag gctagagctt gaatatcaga tggtttctgt ggtttgtgat
     9481 ggtctaattt ctcctgactg agagctaaga ccaagacacg tgatcctctc tccaaggcct
     9541 ccctggcttc tgggacttca gaatccagca acatctcagg tgcccctaag aaaactgtcc
     9601 ccaagccttc taactccata gccccccact tgcggtcgct cgagaaggga agattggaaa
     9661 tcataggata agcaacatct cccacaaaac gctggcgaat ggcttgggca gttggattct
     9721 tatcctcact atgggccatg tagctagtca agatactggc aatagcctct tcaccatacg
     9781 tttccgtcaa cggaagaaca gcctccacct gcatctttcc ttgggtgatg gtacccgtct
     9841 tgtccagaca gagcatatcc acgcgcgcca aggtctcaac agagtacatc tcctgcacca
     9901 agaccttttt caagcccaac ttaatcactg cagtcaagag cgaagtaatg gtcaaaaggg
     9961 caattccctt aggcaacatt cccaaaagag ctgtcgacga gtttacaacg gatgacttga
    10021 gaggcaggcc ttttaaaagc aaggcttcca gcaagagagc cagaccaaag ggaatgataa
    10081 tcttcccagt aaaaccagcc aacttgtcca gcgatttcat gatacgggag ttgatgggtt
    10141 taacggtctt agcctcaagc atgagtttgg cagcatagtt gtctgcaccg acatggtgaa
    10201 cttgagataa aactgaccca ctggctagga aacttcctga caaaagtaag ccgtcaactt
    10261 ccttttgcac caaatcactt tctcccgtta acatggcttc attgacttcc gcaaagcctt
    10321 ccaaaaccaa ggcatcacta ggaatctgct ctcctgcaga caaacgaatg acatctccta
    10381 gcactaattc ttcaggatta agagcaactt cctgaccatc acggatggtt ttgacctttt
    10441 ccttggtcat gagattgagc ttgtccacca tgtgtttggc tcgtagctcg gtcacaatcc
    10501 cagaaaaagc gttaaagcag ataacagcaa agaagaccag attgctccaa gcctgcacaa
    10561 aggcaagagc caaagcaatg gcaaagttca aagcgttaaa aagggtaaag acatttcgtt
    10621 taacgatttg ccaagtgctg gtactggctg atgcggtaaa gtcattgacc aaaccctcag
    10681 cctgtctttc cttgacttct ctttgggtta atcccataat cttattttta tccataaatt
    10741 ctattatatc atttttttta acagaattct aatttcatcc taaatatgta tctaatcaag
    10801 ggaaaagttt gccagccctc ctttgataag gtataataat aacaagaaaa caatcgaagg
    10861 agatcgcatg ttttatactt atttgcgtgg attagttgta ttgctcttat ggtccatcaa
    10921 tggcaatgct cactatcata atactgataa aattcctaat caagatgaaa attatatttt
    10981 agttgcgcct caccgtacct ggtgggatcc tgtttatatg gcctttgcga ccaagccaaa
    11041 acagttcatc tttatggcaa aaaaagaact ctttaccaac cgtatctttg gttggtggat
    11101 tcgtatgtgt ggcgcctttc ccatcgaccg tgaaaatccc agcgcctcag ccatcaaata
    11161 tcctatcaac gttctcaaaa aaagtgaccg ctctctcatc atgtttccaa gtggtagccg
    11221 ccactcaaac gatgtcaagg ggggcgcagc actgattgcc aaaatggcca aggtccgtat
    11281 catgccggtt acctacaccg gtcccatgac tttgaagggc ttgattagcc gtgaacgtgt
    11341 cgatatgaac tttggaaatc caatcgatat ctcagatatc aagaaaatga atgatgaagg
    11401 cattgaaaca gtcgccaatc gtattcaaac agaattccaa cgtctggacg aagaaacgaa
    11461 acaatggcac aatgataaaa aaccaaatcc actctggtgg tttatccgca tccctgccct
    11521 catccttgct attatcctcg ctatcctaac catcatcttt agctttatcg caagcttcat
    11581 ctggaaccca gataagaaaa gagaagaact tgcatagaag aaatgaacct tggccaaaca
    11641 gctaaggttt tcatttatat agtagattgg aactagaata gtacacctct acttctaaaa
    11701 catttttaga aatcgatttg actgtcctga tcgatttgtc ctattcttat ttcattttac
    11761 tatatttata agaatctcct aatcaaatca aaagccactc tacaatagag taactttaag
    11821 attgtccatt tatactcaat gaaaaagccc tctgaaaatc agagagctga tttgagctcg
    11881 agctaaaatc ctagtgaaac aaaaaaatct acacgatcag aaaagtctct atcgtgccat
    11941 tttcatacat gatgtatagt ccaagtagaa tgaatactaa gggcacaatg attcgctcgt
    12001 atttttcaat tgtctcgaat attaacggaa tagaggataa cacccgacta atctcgcaaa
    12061 agataattat gccgattaca aacacaagca aggccacgag ggtctgtgac caatctaacg
    12121 aagcaaaata aggtatatag atacctaaat tatctccgcc agacgcaatt gtcagcaatg
    12181 taactgtcca aaacagttga tttgccttgc tttgttctaa tctttcaata atttcttcct
    12241 cttcttcttc cgcatcttct ccaacaattg caaagcgaat ccctaaatag atagggatta
    12301 aaccaagcaa tccaaccatc cattcttcag gcacgaaatt aacgacataa gcagcaacta
    12361 aactcgcccc tacaagtaag cctgtgccta gatattgccc cgcataaata tgccatttct
    12421 gtttattctg tgatagctgt gcaaataaaa taattaaaat aattaaataa tcgatactgg
    12481 tggaaatata aacaccaata gcagatatga ttgtctgtcc cataaaaaaa actcctgtat
    12541 tagtcagtaa taaatcagca gattttagga gagcacagac acattaattt agctatcgct
    12601 agtgggtaat gtcgaacgta ggaaagcatt ttactcctcc tcaaaacgta gttacaaagt
    12661 aatttctaac tggaattcag tgactacttc catgtaaagt ataacatact atactttact
    12721 ttgtaaagag tgggctcttc tagggggctt gcatacagct actcggcttg aaaagccgag
    12781 taactgcgca cctttatggt gtaattagct gataccgttt gaggtttttg tagtctccaa
    12841 aattgttacc gataaaacct caataaaaaa gccctctgaa gtcagagagc tgatttgagc
    12901 ccgggctaaa atcctagtga aaaagatgaa actccttgtg ctcatcgaac actgtgtcat
    12961 ttccctattt tcatacggat ttttgacgcc cttagcatca tgattcttgc tagataaatc
    13021 gtttatagac taggcgacga ggcgaagatc gtacgaaact ttcagtgaaa caaaaaaaat
    13081 ctccccgaag ggagaaaagc tggtttattt taccttacag tgctaggaac cgtaaccaaa
    13141 gattatactt gagtatatcg aggtaaggtg acaacgcaaa aaagctaccc aatttagagt
    13201 agctttatta tcaagatatg ctcaattgaa cacggcctaa gcacttggca aaaaagataa
    13261 aatctcctag aaactgaagt ttcttcgtca attttcctat ttttgctttg tgcttttgac
    13321 ggcctttgta tcttgaatta gcgacgaagt cctagagagt tgatcaactc acggtaacgg
    13381 ttaacgtcgt ttttacgcaa gtatgcaagc aagttacgac ggcgaccgat ttttttcatc
    13441 aatccacggt aagtagcgtg gtctttttta tgttgtttga tgtgttcgtt aaggtgattg
    13501 atttcccaag taaggacagc aacttgaacc tctactgaac ctgtatcacc ttcgtgacgt
    13561 gcatattgtg caatgatttc attttttttc tcttttgaga ttgccatgat gtttctcctt
    13621 tttatttggc ttcatccgag tgacaggttg gcatgcctgt aaccaagaag aagttatttg
    13681 tctttacgac aatccctatt ttaccaagat ttaaattttc tgtcaaacat taatactctc
    13741 atagttatta tttttcaagt aaagagatac gttcttttat atgatgacct ttttcaagtt
    13801 catctaccaa tccaatagca taatctgcat aactaatctg gcttattcct tttccattag
    13861 tagtaaaaat ttctcctgct agaatttagt ttcctgtttt ttcaccatcg ggaataaaat
    13921 ctacagcagg actgacaaaa gtccagttga gattattttt tgtacgaaga agatccaatt
    13981 catccgcttg agcttttgct agtggcttaa attcttcagg aaaatcaggc gtatctaaaa
    14041 gtcgtgtcat ttttgtttca tctatataaa ggcttcctgc tccccctata actagaaaac
    14101 gtgtctgtgt tccagcaaga agctgattaa atagttcgat tgatttgctg tggagcggta
    14161 gcgtatctgg tgtataagca ccaaacgctg aaataacagc atcaaatcca gtaagatcat
    14221 cttttgtcaa ctcaaataaa tcttttttaa taatagactc agattgactt ttgttttcag
    14281 aacgaacaat agccgttact tcatgtcctc gtttgactac ttcttcaaca attgctttcc
    14341 ccgcttgtcc atttgctgca ataactgcta gtttcatttt ttatacctct cttgttgtaa
    14401 ttattttagt tacagcaatt gtgacactct taataatcaa tgtcaatagt cttgcttaat
    14461 tattatcaaa atatttctac caagaaaact aaccatgatt ctagtgaaaa gaaaaatctt
    14521 ctttgtcaac aaatttactt tcttgtttta aacatgctat aataatcata gcaagagatc
    14581 taagttgtct gtttttttaa aacgaggtga ttatcatgcg tagattctat tcccatctcc
    14641 cctactatct ggtcatatta ttcttttatt ggccacttta tgagttgttc ttgctagttg
    14701 tttctgaccc ccttacacta tataaacaat cttctcttct ttacacctct ggtaatcttg
    14761 attgtatcgt tactctatag ctaccgtttc cgtttctcac tttgatggtt agttggtaac
    14821 ggactgctct tttactttac tatcataacc tttggtgagt ttatactaat ttacttgcta
    14881 atctatgaaa cagttgctct ggtgggcatg gattctggta ttagcatcaa gcatattcta
    14941 caaaaaatga aaaacaaaaa actttcacaa aatccttgaa aaatctcaca atcatgctat
    15001 aataatccat agagacaagt cacttagtcc ctttctacta gagagtgcgt ggttgctgga
    15061 aacgcatagg aagtctaaac tgatactact cttgagtttt ttatgaaaac ataaaacggt
    15121 ggccacgtta gagccaatca gaggtgtccc tctcttttga ggtacataaa tgaaggtgga
    15181 accacgttgc gacgtccttt cgaggatgtc gcatttttta ttaggatact aattatggag
    15241 ttgcaagaat tagtggagcg cagttgggca atccgacaag cttataacga actggaagtt
    15301 aagcatcatg attccaagtg gacggtagaa gaagacctct tggctttatc taatgatatt
    15361 ggaaatttcc aacgactggt gatgacaaaa caaggacgct actatgatga aacaccctac
    15421 acactggaac aaaaactttc agaaaatatc tggtgactat tagaactttc tcaacgtttg
    15481 gatatagata ttctgacgga aatggaaaac ttcctctctg ataaagaaaa gcaattgaac
    15541 gttaggactt ggaagtagtc tgctgataaa aaatcaatgc ttagaaacta tgaaataata
    15601 aaaaaggaga acatcatgat taacattact ttcccagatg gcgctgttcg tgaattcgaa
    15661 tctggcgtaa caacttttga aattgcccaa tctatcagca attccctagc taaaaaagcc
    15721 ttggctggta aattcaacgg caaactcatc gacactactc gcgctatcac tgaagatgga
    15781 agcatcgaaa ttgtgacacc tgatcacgaa gatgcccttc caatcttgcg tcactcagca
    15841 gctcacttgt tcgcccaagc agctcgtcgt cttttcccag acattcactt gggagttggt
    15901 ccagccatcg aagatggttt ctactacgat actgacaata cagctggtca aatctctaac
    15961 gaagaccttc ctcgtatcga agaagaaatg caaaaaatcg tcaaagaaaa cttcccatct
    16021 attcgtgaag aagtgactaa agacgaggca cgtgaaatct tcaaaaatga cccttacaag
    16081 ttggaattga ttgaagaaca ctcagaagac gaaggcggtt tgactatcta tcgtcagggt
    16141 gaatatgtag acctctgccg tggacctcac gttccatcaa caggtcgtat ccaaatcttc
    16201 caccttctcc atgtagctgg tgcgtactgg cgtggaaaca gcgacaatgc tatgatgcaa
    16261 cgtatctacg gtacagcttg gtttgacaag aaagacttga aaaactacct tcaaatgcgt
    16321 gaagaagcta aggaacgtga ccaccgtaaa cttggtaaag agcttgacct ctttatgatt
    16381 tcacaagaag tgggacaagg tttgccattc tggttgccaa atggtgcgac tatccgtcgt
    16441 gaattggaac gctacatcgt aaacaaagag ttggcttctg gctaccaaca cgtctacact
    16501 ccaccacttg cttctgttga gctttacaag acttctggtc actgggatca ttaccaagaa
    16561 gacatgttcc caaccatgga catgggtgac ggggaagaat ttgtccttcg tccaatgaac
    16621 tgtccgcacc acatccaagt tttcaaacac catgttcact cttaccgtga attgccaatc
    16681 cgtatcgctg aaatcggtat gatgcaccgt tacgaaaaat ctggtgccct cactggcctt
    16741 caacgtgtac gtgaaatgtc actcaacgac ggtcacctat tcgttactcc agaacaaatc
    16801 caagaagaat tccaacgtgc ccttcagttg attatcgatg tttatgaaga cttcaacttg
    16861 actgactacc gcttccgcct ctctcttcgt gaccctcaag atactcataa gtactttgat
    16921 aacgatgaga tgtgggaaaa tgcccaaacc atgcttcgtg cagctcttga tgaaatgggc
    16981 gtggactact ttgaagccga aggtgaagca gccttctacg gaccaaaatt ggatatccag
    17041 attaaaactg cccttggaaa agaagaaacc ctttctacta tccaacttga tttcttgttg
    17101 ccagaacgct tcgacctcaa atacatcgga gctgatggcg aagatcaccg tccagtcatg
    17161 atccaccgtg gggttatctc aactatggaa cgcttcacag ctatcttgat tgagaactac
    17221 aagggggcct tcccaacatg gctggcacca caccaagtaa ccctcatccc agtatctaac
    17281 gaaaaacacg tggactacgc ttgggaagtg gccaagaaac tccgtgaccg cggtgtccgt
    17341 gcagacgtag atgagcgcaa tgaaaaaatg cagttcaaga tccgtgcttc acaaaccagc
    17401 aagattcctt accaattaat tgttggagac aaagaaatgg aagacgaaac agtcaacgtt
    17461 cgtcgctacg gccaaaaaga aacacaaact gtctcagttg ataattttgt tcaagctatc
    17521 ctagctgata tcgccaacaa atcacgcgtt gagaaataag agtctagcat aaaagcctcc
    17581 aatctggagg ctttttctca tctattttta ctcaaggact aagttcactt gagcaaactg
    17641 aatccgcact gtcgttcctt tcccgacctc agactcgata cgaatctgat gccccagttc
    17701 ttcagaaatt ttcttagata gataaaggcc aagtccagag gactgctggg tcaaacggcc
    17761 attgtatcct gaaaagccac gttcaaatac tcggagaaca tcactgtttt ttatcccgat
    17821 tcccgtatct ttgatacaaa gctcttggtc atccatataa atctccagac caccttcctt
    17881 ggtgtacttg agactgtttg agatgatttg ctcaataacc actagcagcc actttttatc
    17941 cgtcacgatt tctttatcaa ggtcatgtag attgacattt aagccttttt gaataaagaa
    18001 aagagcatat ttacgaatta tttccttgac caagtcctca atttgaacct gctttaagag
    18061 caaatcatca tggaaacttt ctaaacgcag gtactgtaaa actaggttgg tataggagtc
    18121 gattttgaaa atttcctgtt ctagctgctg cttcagttgg cggtcgacca cttctgcaac
    18181 taagagttga ctggctgcaa tgggggtctt tatctgatgg acccacaagg tatagtaatc
    18241 cagcaaatcc gtcagttttc tttctgcttt tgacctctgc tgatagagtt ccatctcacg
    18301 cgcttctaat ttttctgcta aagctatttc caaaggagac ttggcttccc tctctccata
    18361 gagaagttcc tggcgataga cctgcatttc caccaatatg tcccaagtga aaaataagat
    18421 ggttacaaag caacacaaga ggaaaaagta gaggaagtaa attcctagac tggcaaataa
    18481 aaactgaaag agtaagacca gaaatgccaa agaaagcaga tagacaaaaa gacgactacg
    18541 ggagcgcaga taggctagaa aaaattgttt ccaatcaagc atgcttcaat ccgtacccta
    18601 ttcctttctt ggtctcgata aatcctacca atccctgctc ctccaacttt ttacgcaaac
    18661 gagccacatt gacagagagg gtattatcat caatgaaaaa gtcactgttc caaagttctc
    18721 gcatcaggtc gtcacgtgct acgatgttgc ctgcatgctc aaataacacg cgtaaaatct
    18781 ggaattcatt cttggtcaaa ttcaagactt gcccttgata atgtaaatcc atggatttgg
    18841 tattgaggat aacaccagca tattccagca aactctcatc acgcccaaac tcataggaac
    18901 gacgcaacaa gccctgaacc ttagctaaaa gaacctgctg gtcaaaaggc ttggtcacaa
    18961 agtcatccgc ccccatattg attgccatga caatatccat agcctggtct ctcgaagaaa
    19021 gaaacatgat aggtaccttg gaaatcttgc ggatttcctg gcaccagtga taaccattaa
    19081 acaagggcaa accaatatcc atgaggacca gatgaggttc cgactgaaca aatatactca
    19141 aaacttccat aaagtcttct accaggacca cttcaaatcc ccattcagag agcattttcc
    19201 caatctgttg acgaatgacc tgatcatctt ctattaataa aatcttgtgc atacgcttct
    19261 ccttttccat tattataaca gatttttcca tgctagatgg tctgaaactg aatttgaaat
    19321 agcctgtttt tagccagtac aaacaggcta tgctactagc taatttgagg gaaatttgct
    19381 aagataaata aaaagaaagg agctcttatg gccaatattt ttgactatct gaaagatgtc
    19441 gcatatgatt cttattacga ccttcccttg aatgagttag acattctaac cttaatagaa
    19501 atcacctacc tctcctttga taatctggtc tccacacttc ctcaacgtct tttagatcta
    19561 gcacctcagg ttccaagaga gcccaccatg cttactagca aaaatcgcct tcaattatta
    19621 gatgaattgg ctcaacacaa gcgcttcaaa aattgcaaac tctcccattt tatcaacgac
    19681 atcgaccctg aactgcaaaa gcaatttgcg gctatgactt atcgtgtcag cctcgatacc
    19741 tatctgattg tctttcgtgg gacagatgac agtatcattg gctggaagga agatttccac
    19801 ctgacctata tgaaggaaat tcctgctcaa aagcacgccc ttcgctattt aaagaacttt
    19861 tttgcccatc atcctaagca aaaggttatt ctggctgggc attccaaggg aggaaatctc
    19921 gctatctatg ctgctagcca aattgagcaa agtttgcaaa atcagatcac agcagtttat
    19981 acatttgatg cacctggtct ccatcaagaa ttgacacaga ctgcgggtta tcaaaggata
    20041 atggatagaa gcaagatatt cattccacaa gggtctatta tcggtatgat gctggaaatt
    20101 cctgctcacc aaatcatcgt tcagagtact gccctgggtg gcatcgccca gcacgatacc
    20161 tttagttggc agattgagga caagcacttc gttcaactgg ataagaccaa cagtgatagc
    20221 cggcaagtag acacaacctt taaagaatgg gtggccacag tccctgacga agaacttcag
    20281 ctctacttcg acctcttctt tggcactatt cttgatgctg gtattagctc tatcaatgac
    20341 ttggcttcct taaaggcgct tgaatacatt catcatctct ttgtccaagc tcaatccctc
    20401 actccagaag aaagagaaac cttgggtcgc cttacccagt tattgattga tactcgttac
    20461 catgcatgga aaaatagata atactcttga aaattaaatg tatacaaaac aaaagaccta
    20521 gaatacatac tttcatgtgc attctaagtc tttttaaata gaatctaata gtcaataaaa
    20581 atcaaagagc attgagagat aatggggctt ggaacgtccc tctcgtttca acaaaatgac
    20641 cccattatag attaaaaaga tgccacttag aaaaagcaaa aaaggaagta agacaaaggc
    20701 aaatacataa aaagctaact gaacattctc gtatccattt ttataaaaaa ggtaggatag
    20761 ataaaaataa cttgaaatga gggataataa aaataatact ggattccaca aacttctatt
    20821 atccttccaa aatgacacta taaaggctaa tacaatttct ataacgagat acatttctta
    20881 ctcctttaat agctacattt tatcataatt atccaaagaa aaaaagaggg catttatccc
    20941 tcttaatcct tcatctgact ctctgcatcg gccacgactt tttctagact ggtctgacca
    21001 agttctgcct ccatagtcaa ctgaattctc tccaattttt gatccaaaac atcatgaata
    21061 tgagctccta cagggcaatt tggattcgga ttgtcatgga aactgaagag ttgacctgtc
    21121 ttaccaagac attcgaccgc ctgataaaca tctaaaagac taatatcctt aaggtccttg
    21181 acaatctctg ttccgcccgt tccacgcgct actgaaatca gctctgcctt cttcaactgg
    21241 gacaagatct ttctgataat gacaggattg accccaacac tagcagccag aaaatcactg
    21301 gtcaccttgc tttccttccc ctcgagggca atgattatca gcatatgagt cgcaatggta
    21361 aatctacttg gaatttgcat cctcttctcc tttttacgag gctaccctgc ctctactctt
    21421 ctttttctat tattataccc tttttagttg taatgtcaat cgttaccact tttcaaccag
    21481 tcgtctaact cccgatcgca accctctttc tgagccaatt ctctcaaaaa ttcctgatga
    21541 tgagtatggt ggatcccatt gaccagactt tcatagtaaa cctcaaaata gggaagtctc
    21601 aggtctttag ccagctgcaa ttcagctgct acatcgtagt ctacccgtcg gaagtccata
    21661 tctaccaggc ctttgtcatc aaactccaaa atcatatact gggcccgcaa gtccttccgt
    21721 agctgagcgt ccaaaaagaa aggttggcca atcgaacccg gattgacaat caattgccca
    21781 ccagtcccgt aacgaagcaa ctgctggtga atatgtccat aaacagcaat atcacaggga
    21841 ggatgagtca ccaagcggtc aaactcctct tgtttgccag tatgaatcaa ctctcgcccc
    21901 cagttcttat caggcagatg atggctaatt cccaccgtca aatccccaaa ctgacgatga
    21961 atttgaagag gttgattgtg gagcacttca atttcttcta gggaaatttc ctctaaaaca
    22021 tactggcact ggcgcaagag atagcgttga ctggggcgag tactatccaa ttccttacgg
    22081 acaccatgcc aaagactgtc ttcccagttt cccaaaactc tagccgtaat cggtagttga
    22141 tccaacaagt ccaaaatcct tctacgccct gtccctggca tgagaatatc tcccaaaagc
    22201 cagtattcat ccactcctat ctgccgagca tctgccaaaa cagcctccaa ggcggtggta
    22261 tttccatgaa tatctgaaag aagagctatt ttcgtcatat ccatctcctc gttttttctc
    22321 ttgcaataag tataacataa aaagtcacag ctagagaaat ctagcttttt ttgatatact
    22381 agataaagat attagacaag aggaaacgaa tgaccccaaa caaagaagac tatctaaaat
    22441 gtatttatga aattggcata gacctgcata agattaccaa caaggaaatt gcggctcgca
    22501 tgcaagtctc tccccctgcc gtaactgaaa tgatcaaacg aatgaaaagt gaaaatctca
    22561 tcctaaagga caaggaatgt ggctatctac tgactgacct cggtctcaaa ctggtctctg
    22621 agctctatcg taagcaccgc ttgattgaag tttttctagt tcatcattta gactatacaa
    22681 gtgaccagat tcacgaggaa gctgaggtct tggaacacac tgtctctgac ctgttcgtgg
    22741 aaagactaga taaactgcta ggtttcccta aaacctgccc ccacggagga actattcctg
    22801 ccaagggaga actcctcgtt gaaatcaata acctcacact agctgatatc aaggaagctg
    22861 gcgcctaccg cctgactcgg gtgcacgata gttttgacat tctccattat ctggacaagc
    22921 actcacttca catcggtgac cagctccaag tcaagcagtt tgatggcttc agcaatacct
    22981 tcactatcct cagtaacgac gaggatttac aagtgaatat ggacattgca aaacaactct
    23041 atgtcgagaa aatcaactaa tttctcaagt cccctaccaa ccctgaaagt tttattttca
    23101 gggttttatt tctatcattt gattttactt tcatttggtg gtagagtatt tgaaaaaaaa
    23161 aaagaaagat agtttaacct atgaaaaaat cactaaaaat ttttgctaca tctaaatagt
    23221 ttgacctctt cggggttgct ttggtcgttg ggattgcgat tgcatctggt tacctcaact
    23281 cacacctcga taaatttgta gactggggat catggacagc tcttgtaccc tttggattga
    23341 tttccgtaac caacgttggg atttccatgt tgtccactcg tttcacgggg aaattaagca
    23401 aatggggaaa ttactttggt attgttaata ccattttgtc cggtgctatt gattatatcc
    23461 ttggaaataa ggcggccatc attacctatc ccgtcacctt cctcatttat acctttgcta
    23521 taaagaagtg ggaagcttcg caagaaggca gacccaacca aatgagccaa aaacaggtaa
    23581 aattggcggc catcatcatt tccatcatcg ccttcctctt tgcctttgtg accaactata
    23641 tcggatatgg gggcaagatg aatctccttg cctacgtaac aactattgcc tttgcactgt
    23701 ccctcattgc caatgctttg aatgcattaa aactaacaac tcagtggggc ttttggttga
    23761 tttacaattt cgttcagctg acaaaagctg gtattcaagg aaacttcgcc aatatcggta
    23821 aatacatctt ttatatcctc aatgcaatcg gagctttatt tgtctggaat gatgaagaag
    23881 ttagataata gaaggaaact caaataaaga atactagcca acttaagaga atctcccttc
    23941 tgctattttt atcattttag ttgtaactcc cattcttttg gagtagaatg aagttagata
    24001 aaagagggag gtcttgtcat gaaaaaactc tatagaatcc attttatagc aattgcagtt
    24061 attgatttgc tactatttgc attttttatc actagactcg agacatcttt tgagtggctc
    24121 ttgctctctg gtttaatttt cttccttgct caaggactcc tgctatttct cttggtcgtc
    24181 cgactcaaac atcaattcgc tgagatttat cctcaaatca ataaaaagat tcgcttctac
    24241 tatttagggg ttctcaccat tgattttcta ttttttgttc tcttagcctt cattagttct
    24301 cagcgttttt catctcttat gccaatcatc actgcttgcc attctacttt ttattatatg
    24361 acagctgact acctaagaga aaactatcca gacttttacg acaaacacat ctctttatgg
    24421 gagtgtctct aaggaaaagg aggttttagc atgaaaaaaa tcatcttcat caaaaccatt
    24481 caactccttg tcattgatgg aatcatgctg gcatttttga catttaaaag ggggcttact
    24541 tgggactgga ttttgattta tagcggttgg ctcattttct ttcatcctgt gctattgacc
    24601 tatctttcaa accaactttg tgaccacttt agttaactct attcccagat tagaccgaga
    24661 ttctggcgtt ttgctttaca aattctccta tgggatagcc tgatgattct ctccttggtg
    24721 tctttaagtg atattccact tttccttcag ggaactctcc tcatcctagg acatctcatc
    24781 ccttcctatc gcatctgcca aagcctgaaa agagacttcc cccaagcata tcaagaaccg
    24841 atttcttttt ggagtatttt atgatcgatg agaaagacca agccgactgg gcttggtctt
    24901 tcttatctct ttttagtatc taggataatg gtaacaggtc cattattaac cagctcaacc
    24961 tgcatatctg ctccaaagat gcctgtctga acgggcactt cttgcgctaa tttttgattg
    25021 aaagcatcat agaagtctga tgccatatca ggtttagctg cccctgtaaa ggctggacga
    25081 ttgcctttct tagtatccgc aaagagggta aactgagaaa tagagaggat ttctccttca
    25141 atatctttga cagacaggtt catcttgcct tctgcgtctg aaaaaatccg catattgacc
    25201 agttttctca cagcatagtc caaatcttcc tcttggtcct ctggtccaac accaaccagc
    25261 aataaaagtc cctgattgat ttttccctga atctggcctt ctatactcac ttgggctttt
    25321 ttaacccgtt ggataatgat tttcataata gcctttctag taagagctag gacaactagc
    25381 cgttggtccg tttgacagag taaacttctg gcacactctt aattttatcg acaaccgtgg
    25441 tcagtgtaga gaggttggca ataccgaagg acacatggat attagcaaac ttcatatcct
    25501 tggttggttg ggcattgacc gttgaaatat tcttggttgt atttgaaaga acttgcagta
    25561 catcgttcaa cagtcctgta cggttgagac cgtagatatc gatatgggcc atatactcct
    25621 tatttgagct agagtactgg tcttcccatt ccacatcaag gagacgttgc tcgtagtttt
    25681 cttgggcacg caggttcata cagtccacac ggtgaatagc cacaccacga cccttggtaa
    25741 tgtagccaac aatatcgtca ccaggcacgg ggttacaaca cttagcaatc cgcactagga
    25801 gaccagaagc accttcaata accactcccc cctcatgctt gaccttgagg gtttctttat
    25861 tttcaacctt gacctcgcca cctttgacaa gctcctctgc ctcagctttg gccttggcac
    25921 gctcttcctc acggcgttcc ttttcagtca gacggttaaa gacggtaatc gcaccgattt
    25981 ccccaaaacc aatggccgca aagagggagt cttctgtctt gtaactggtc ttttgcagaa
    26041 cttgatccat gtggcgcttg tccataaatt tatttgccac atagccattt tcttggaact
    26101 gagccatcag catctcacga cccttgttga cagacaattc cttatcttgg tttttaaaga
    26161 actggcgaat cttattgcgc gccttgctag tcttgaccat attgagccag tcacggctag
    26221 gtccaaagga gttcgggttg gcgataattt caacctgatc ccctgtcttt aacttggttg
    26281 tcagtggaac catgcggcca ttgaccttgg caccagttgc tttttcaccg accttggtat
    26341 ggatttcgta ggcaaaatca atcggtcctg aatctttggg aagggaacgg acagctccat
    26401 ctggggtaaa aacgtaaatc tcctcagcca aatagttttc cttaacagag tccacaaatt
    26461 ccttagcatc atcagcctgg tcttggagct ccatcatctc cttgatccag ttcattccaa
    26521 tagctgattc cttgctgtta acttgcccct ttataccttt cttataagcc cagtgagccg
    26581 caaccccgta ctcagccacc tcgtgcattt ccttggttcg aatctggaat tcaatcggcc
    26641 cttttggtcc ataaacagtc gtatggatag actgataacc attggccttg cggttggcaa
    26701 tatagtcttt gaagcgacct ggcatcggtt tccaaaattc atgcacgtaa ccaagcatgg
    26761 cataaacatc actttgggta tctaaaatac aacgaatagc aatcagatca tagatttcct
    26821 caaaccgttt tctcttgtcc tgcattttgc ggaaaattga gtaaatatgc ttgggacgac
    26881 cataaatctt ccctttcaag tgacgttctg tcgtatactc ctctaatttt gtgactacct
    26941 catccaccaa ggcctcacgc tccctgcgct tttccttcat catatgggta atcttgtaaa
    27001 actccgttgg attgagataa cggaaagaca agtcttctaa ttcccatttg acactggaaa
    27061 tccccaaacg atgggcaagt ggggcataga tttccatggt ttctttggaa atacgctcct
    27121 gcttgtcttt tcgaagatgt ttcagggtcc gcatattgtg caagcggtca gacagtttga
    27181 ccaaaataac gcggatgtcc tcagacatgg ccatgagcat cttgcgatga ttttccgcta
    27241 attgctcctc gatcgatttg tactcgacct tgccaagctt ggtaactccg tcaacaatca
    27301 cccgcacatc aggaccaaac tctctttcca aatcgtccaa ggtcgcatct gtatcttcca
    27361 ccacatcatg caagaatcca caagctactg ttacagcatc cagctttagc ttagctaaaa
    27421 tacctgccac ttggataggg tgaatgatat aaggctcgcc tgatttgcga tattgaccac
    27481 tgtggcattc aacagcatag accaaggcct tatggacaaa atgaacatcc tcttccgtta
    27541 aatattcttt ggttaaagcg acaacttctt cgcctgttaa attcacttct ttcggcatct
    27601 ctactctcca attcttccta ccattttatc acttttttaa gaatatgaaa actagattgg
    27661 aacagaataa gaaaaaaata attcaaaatt gcttgataat tctgaattat tggcccataa
    27721 tatactacga agttagattt taaacttagg tgatagaagg agagatagaa gaacggaaac
    27781 catattgtaa cccaaagact ttctgacttc cccaattcca ttgaagatac gaaagataaa
    27841 cggtggaact cgtatcacat acactggtac cttgactgga ttttagaatt aatactcaat
    27901 gaaaatcaaa gagcaaacta ggaaactagc cgcaggttgc tcaaagcact gctttgaggt
    27961 tgtagataag actgacaaag tcagtcacat atatacggca aggcgacgtt gacgcggttt
    28021 gaagagattt tcgaagagta taaaaatcct caagatactt tcttctatcc tttagtttat
    28081 aaggagaata cctatgaaaa aaactgctat ttctatcttt gctctcctaa tgttaggagt
    28141 ttgctgcctg ttcctattca gccagcaaag ctataaaaaa cagtcgttca atactatgct
    28201 aacgaccaga acctacccag taggataact tatagtgaat atagcgacaa atgagaagcc
    28261 aactacggta gcactctaaa catcacgtct atcaaacaag ctaatgacgg agtttatgca
    28321 acctatgaag ggcaattgac acctttccaa tattgataaa ttgataacca gcctgtcttc
    28381 atctagtcat gctggttttt aagttcattt taaatcctta cctattctcc ctaactgtgc
    28441 tatacttaat ttatactcaa tgaaaatcaa agagcaaact agaaagctag ccgcaggctg
    28501 ttcaaagcac tgctttgagg ttgcagataa agttgacgcg gtttgaagag attttcgaag
    28561 agtattagta cattctttga gattggagct agtatgaaaa tccataaaac cgtgaatcct
    28621 gttgcctatg aaaataccta ttatctagaa ggcgaaaaac acctcatcgt cgtcgatcct
    28681 ggtagttatt gggaagccat tcgtcagaca atcgagaaga tcaacaaacc gatctgtgct
    28741 attctcttga cccacgccca ttatgaccat atcatgagtc tggacttggt tcgcgagacg
    28801 tttggcaatc ctcctgtcta tatcgcagag agcgaagcca gctggctcta cactcctgtc
    28861 gataatctat ccggtctccc tcgccacgat gatatggcag atgtggtcac aaaacctgca
    28921 gaacacacct ttgtctttca cgaagaatac caactagagg aatttcgttt taaggtttta
    28981 ccgaccccag ggcactctat cggtggtgtt tccctagtct ttcctgatgc tcacctagtc
    29041 ttgacgggag atgctctatt ccgcgaaact atcggacgga ccgaccttcc gactggtagc
    29101 atggagcaac tccttcatag tatccagacc caactcttca ccctaccaaa ctacgatgtc
    29161 tatccaggac atggtccagc tactactatc gctcacgaaa aggccttcaa tccctttttc
    29221 tagcaagatg atgacaatcg aaatttaagt aaactatcca gcaaatcttt ctattacaaa
    29281 aggcatccta tcaaggtttt cacacgtgat tggatgcctt ttctctgatg actagatttt
    29341 ttgtattacc aaataatcac gcgctcctct ggtgaacgcc acattccgtc tccttctttg
    29401 acatcatagg ttgtaaagaa atcgtcgaag tttggtactt gcacattgac acggagtttg
    29461 gctggtgcgt gcacatcgac gctagccaaa agtttcataa attctggacg acctttcatg
    29521 cgccagatgc gaccgaagtt gtagaagaac tcttctgctg agaagtctgc ttctctctta
    29581 gctgcttcaa gcgctgctgc gattcctccc aagtcagcca cgttttctga tacagtcaat
    29641 ttaccgttaa tggttgctcc ataagaatcc tgtccatcaa attggtcaat gactttttgt
    29701 gttttctcct tgaaggcagc atagtcgctc tctgtccacc aatccttgag actaccattt
    29761 tcgtcaaagg aagccccgtt agtatcaaag gcgtgggaaa tttcatgggc aatcactgcc
    29821 ccaataccac cgtagttagc agaagatgac tgatgcaagt catagaaagg cgcctgtaaa
    29881 atggccgctg gaaagacaat caggttcttc tgaggattgt agtaggcatt gaccatatga
    29941 gcaggcatgc cccattcctt atagtctaca ggctggttcc acttactcca actgtgcttg
    30001 atttccacac gcgcaaaggc tagagcattc tcaaaaagac tggcagtttc attcactacc
    30061 ttatccttgt aacgtgcagg caattcttct ggatagccaa tataaggttt gatcacattg
    30121 agcttcacga tagcctgttt acaggtttct ggagtgagcc agtcattctt aagcagacgc
    30181 tccttataga catcaatcat ggttgccact tttttctcca catccgcctt ggcttctgga
    30241 gagaacttct cacgggcgta ccaaagcccc agggcttgct tgaaaggttc ctgtgctaga
    30301 tgataagctg ctttgacctt atcttttgcc tctggaactc cagaaagggc acggctgtag
    30361 gcaccagaca aaacacggat atcctctgtt aaatagctgg ttgaaagatt gacaacactc
    30421 aaaatcaagg ttgctttaag gagagaccag gcttcctcac tgtaaaattg ctctgctgct
    30481 tgccagaaac gttcctcgtc tacaataacc ttgtctggta attgcccaat aactgctttg
    30541 aagaagtcat ccaaaggtag ggcaggcgcg aatttcttga aatcttcgta agaatatgga
    30601 tgatagagtt tagcatattc tgaactttct tcattagaga gcaccactgc cgcaactcgg
    30661 cggtccaatt caagtctttt ttctagcaag tcttcaattt cttcatcaga gaaatcataa
    30721 gccttgagga gatttgcgct actttctttc caaagagtca agagctcttc gcgctgagga
    30781 tgttcttctg catagtaggt cgtatctggc aagattgtgc ttggagcgct agcccataga
    30841 acattgattc tagcatccat aaagtctggc gatacaccaa aaggaaggaa gtttggtttt
    30901 cctgcaagct caaactctgc tagtttagct gtaaaatccg caaaagtttc caattcttgg
    30961 aattctttaa ggagtggtaa gacaggtgtg ataccgtcag cttctctctt gtcaaaatca
    31021 cgaactaggc ggtggtattt gacaaagttt tccaagatag catcctcagg cacttcttca
    31081 cctgctaacc acttgtctgt tgtcgccagc atcaggtctt caatttcctg gtctaaatca
    31141 acaaaacctc ctgtttgaga cttatctgct gggatttcag ctgtctgttg ccattctcca
    31201 ttgatagcat cataaaaatc atcttgataa cgtgtcatct tgttctcgct ttcatttgta
    31261 tttgcattta tcttaacaaa aatcactagg gaatgcaatt aataaccatt taactattat
    31321 tttttagcta ttataaaaaa cctttccatg ataaaa
//
LOCUS       DAVLVZ010000020        29891 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000020 DAVLVZ010000000
VERSION     DAVLVZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.020"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            95..778
                     /locus_tag="VYN77_000883"
     CDS             95..778
                     /locus_tag="VYN77_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1558363.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            778..1512
                     /locus_tag="VYN77_000884"
     CDS             778..1512
                     /locus_tag="VYN77_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1558364.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGISLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKAKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            1666..2523
                     /locus_tag="VYN77_000885"
     CDS             1666..2523
                     /locus_tag="VYN77_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1558365.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            2665..3537
                     /locus_tag="VYN77_000886"
     CDS             2665..3537
                     /locus_tag="VYN77_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1558366.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPESMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            3819..4502
                     /gene="rpiA"
                     /locus_tag="VYN77_000887"
     CDS             3819..4502
                     /gene="rpiA"
                     /locus_tag="VYN77_000887"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1558367.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            4516..5727
                     /locus_tag="VYN77_000888"
     CDS             4516..5727
                     /locus_tag="VYN77_000888"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1558368.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            5729..6271
                     /locus_tag="VYN77_000889"
     CDS             5729..6271
                     /locus_tag="VYN77_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV1558369.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFASIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELKNLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            6287..7096
                     /locus_tag="VYN77_000890"
     CDS             6287..7096
                     /locus_tag="VYN77_000890"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1558370.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            7572..8552
                     /locus_tag="VYN77_000891"
                     /pseudo
     CDS             7572..8552
                     /locus_tag="VYN77_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            8577..9089
                     /locus_tag="VYN77_000892"
     CDS             8577..9089
                     /locus_tag="VYN77_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1558371.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            9747..10457
                     /gene="deoD"
                     /locus_tag="VYN77_000893"
     CDS             9747..10457
                     /gene="deoD"
                     /locus_tag="VYN77_000893"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1558372.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(10480..11022)
                     /locus_tag="VYN77_000894"
     CDS             complement(10480..11022)
                     /locus_tag="VYN77_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV1558373.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCRMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTI
                     LRNQRELDQFLDKKRKSYNS"
     gene            complement(11075..11311)
                     /locus_tag="VYN77_000895"
     CDS             complement(11075..11311)
                     /locus_tag="VYN77_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1558374.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(11379..12299)
                     /locus_tag="VYN77_000896"
     CDS             complement(11379..12299)
                     /locus_tag="VYN77_000896"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1558375.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            12497..12937
                     /locus_tag="VYN77_000897"
     CDS             12497..12937
                     /locus_tag="VYN77_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558376.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIAAGSYCFLVLWIATEGYFF
                     RMSLEDLIRLWTSLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            13153..13734
                     /locus_tag="VYN77_000898"
     CDS             13153..13734
                     /locus_tag="VYN77_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1558377.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETSGDLTIVIQKKQ
                     GAPSAKSKMEGVFGNCEILKKDKGYYILRSVKE"
     gene            13731..15008
                     /locus_tag="VYN77_000899"
     CDS             13731..15008
                     /locus_tag="VYN77_000899"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1558378.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILVPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            15026..15688
                     /gene="deoC"
                     /locus_tag="VYN77_000900"
     CDS             15026..15688
                     /gene="deoC"
                     /locus_tag="VYN77_000900"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1558379.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            15675..16064
                     /locus_tag="VYN77_000901"
     CDS             15675..16064
                     /locus_tag="VYN77_000901"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1558380.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            16154..17206
                     /locus_tag="VYN77_000902"
     CDS             16154..17206
                     /locus_tag="VYN77_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1558381.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            17350..18885
                     /locus_tag="VYN77_000903"
     CDS             17350..18885
                     /locus_tag="VYN77_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558382.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            18878..19936
                     /locus_tag="VYN77_000904"
     CDS             18878..19936
                     /locus_tag="VYN77_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1558383.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            19939..20895
                     /locus_tag="VYN77_000905"
     CDS             19939..20895
                     /locus_tag="VYN77_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1558384.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            20980..22327
                     /locus_tag="VYN77_000906"
                     /pseudo
     CDS             20980..22327
                     /locus_tag="VYN77_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(22370..23011)
                     /gene="plsY"
                     /locus_tag="VYN77_000907"
     CDS             complement(22370..23011)
                     /gene="plsY"
                     /locus_tag="VYN77_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1558385.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            23147..25090
                     /gene="parE"
                     /locus_tag="VYN77_000908"
     CDS             23147..25090
                     /gene="parE"
                     /locus_tag="VYN77_000908"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1558386.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTALKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILMPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            25510..27981
                     /gene="parC"
                     /locus_tag="VYN77_000909"
     CDS             25510..27981
                     /gene="parC"
                     /locus_tag="VYN77_000909"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1558387.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLRIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKNFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            28111..29133
                     /locus_tag="VYN77_000910"
     CDS             28111..29133
                     /locus_tag="VYN77_000910"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1558388.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            29189..>29891
                     /locus_tag="VYN77_000911"
     CDS             29189..>29891
                     /locus_tag="VYN77_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1558389.1"
                     /translation="MVLSTLAILVACGKTDKEADAPTTFSYVYAVDPASLGYSITTRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPIVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
BASE COUNT         9006 a         5380 c         6829 g         8676 t
ORIGIN      
        1 taaagatttt taaataaatt ggataaaaaa tttaaatttt aattcaaaac atgttataat
       61 catacagtat tctattttag aaagcagtgt gactatgaat ttttcttttt tacctaagta
      121 tttaccttat tttaactatg gggctgttgt gacgattctt atttctatct gtgttatctt
      181 tttgggaact attttgggtg ttgtcttggc ttttgggcaa cgttcaaagt ttaaaccgct
      241 tgtttggttg gccaacttgt acgtttggat tttccgtggg acaccgatga tggttcaaat
      301 tatgattgcc tttgctctta tgcatatcaa tgctccgact attcagattg gaattttagg
      361 tgttgatttt tcgcgtctga ttccagggat tttgattatc tctatgaata gtggtgctta
      421 tgtttcggag actgttcgtg ccggaatcaa tgcggttcca aaaggtcagc tagaggcggc
      481 ttattcgcta gggattcgtc ctaaaaatgc gatgcgttat gtgattttgc cacaagcaat
      541 caaaaatatc ttgccagcat tggggaacga atttatcacc attatcaagg acagctccct
      601 cttatcagct attggggtca tggagttgtg gaatggggct acaacagttt ctacaacaac
      661 ctatctacct ttaacaccac ttttatttgc agcattttac tacttgatta tgacctctat
      721 tctgacagta gccttgaaag cttttgaaaa acatatggga caaggagata agaaataatg
      781 acagaaacct tgataaaaat tgaaaattta cataaatctt ttggaaagaa tgaagtattg
      841 aagggcatca gcctcgagat taaaagagga gaagttgtcg ttatcatcgg tccttcaggg
      901 agtgggaaat ctaccttgct tcgctctatg aatttgttgg aggaagcaac caaggggaag
      961 gttatctttg agggagtcga tattacggac aagaagaatg acctgtttgc catgcgtgag
     1021 aagatgggca tggttttcca acaatttaac ctctttccta atatgactgt gatggaaaat
     1081 attaccttgt cacctatcaa ggctaaaggt gaaagcaggg aagttgctga gaagagagcc
     1141 caagagcttt tggaaaaagt tggtttgcca gataaggcag acgcttatcc acagagtctg
     1201 tcgggtgggc aacaacaacg gattgccatc gcgcgtgggt tggctatgga accagatgtt
     1261 ttgctctttg acgaaccaac ttcagccttg gatcctgaaa tggtaggtga ggtattggct
     1321 gttatgcaag accttgccaa gtcagggatg actatggtta tcgtaacaca tgagatggga
     1381 tttgcccgtg aggtggcaga tcgtgttatc tttatggcag acggtgtggt tgttgaagat
     1441 gggacacctg agcagatttt tgaacaaacc caagaacaac ggactaaaga tttcttgagt
     1501 aaggttttat aatctatttt aaaatttcaa gacaagatta gtgaaaactt caaccagagc
     1561 tttttcttat agtttgaaac tataggataa cctaggaaag aagtgttaga gggcactagc
     1621 agtttttggt ataatggaag atatttgaaa gaaaagagaa gtgatatgac acagattatt
     1681 gatgggaaag ctttagcggc caaattgcag gggcagttgg ctgaaaagac tgcaaaatta
     1741 aaggaagaaa cagctctagt gcctggtttg gtagtgattt tggttgggga taatccagcc
     1801 agccaagtct acgttcgcaa caaggagagg tcagctcttg cggctggttc ccgtagcgaa
     1861 gtagtgcgag ttccagagac cattactcaa gaggaattgt tagacctgat tgctaaatat
     1921 aatcaggatc cagcttggca tgggattttg gtccagttgc cattaccaaa acatatcgat
     1981 gaagaggcgg ttttattagc cattgaccca gaaaaggatg tggatggttt ccatccccta
     2041 aacatgggac gtctttggtc tggacatcca gtcatgattc cctcgacacc tgcaggaatt
     2101 atggaaatgt ttcatgaata tgggattgac ttggaaggta aaaatgcggt cgtaatcggt
     2161 cgttcaaata tcgttggaaa acctatggct cagcttcttt tggctaaaaa tgcgacagta
     2221 accttggccc actcacgaac tcataatctt gccaaggtgg ctgctaaagc agatattctt
     2281 gtagtcgcaa tcggccgcgc caagtttgtg actgctgact ttgtcaaacc aggtgcggtt
     2341 gtcattgacg ttgggatgaa tcgagatgaa aatgggaagc tctgtggaga tgttgattat
     2401 gaggcagttg caccacttgc tagccatatc acgccagtcc ctggaggtgt cggtcctatg
     2461 accattacca tgctgatgga gcaaacttat caagcagcac ttcggacatt ggatagaaaa
     2521 taagagaaac attcgattaa tagagtgtat tttctatagc tatatctaaa atggcagaaa
     2581 tgaatattaa attttagata taagattaca aaaggaggtc tgcgtctcct ttttattgta
     2641 taataaaagt gagaggaaaa aaggatgaaa gtgattaatc aaaccttatt agaaaaagtt
     2701 attattgaac gttctcgttc aagtcataaa ggagattatg gtcgtctgct gctgcttggt
     2761 ggtacttatc cttatggagg tgccatcatc atggctgctt tagcagctgt aaaaagcggt
     2821 gcaggattgg tgaccgttgg aacggacagg gaaaatatcc cggctctgca cagccattta
     2881 cctgagtcta tggccttttc tcttcaagac cagcaattgt taaaagagca attggagaag
     2941 gcagaagttg tcttgctggg gcctggttta cgagacgatg cttctggaga aaatctagta
     3001 aaacaggtct ttgttaattt aagccaaaat cagattttga ttgtagacgg aggtgccttg
     3061 accattcttg ctaggacaag tttgtcattt ccctcaagcc agcttatcct aactcctcac
     3121 caaaaagaat gggaaaaatt atctggaatt acgattgaaa agcaaaagga agatgcaacg
     3181 gctagtgttc tgacttcctt ccctcaagga acaattttgg tggagaaagg cccagctact
     3241 cgtatttggg aagttggcca atctgattat taccagttac aggttggtgg tccttatcag
     3301 gcgactgggg gaatgggaga tactctggct gggatgatcg caggatttgt aggccaattc
     3361 cgacaggcca gtctctacga gcgtgtggca gtagcgaccc atcttcattc agccatagcc
     3421 caagaactat ctcaagaaaa ttatgtggtc ttaccgacgg aaatcagcag gtatcttccc
     3481 aaaataatga aaattatttg tcaacaagag agggtaagca aagataaact tgtttaaaat
     3541 agacagataa atctacttcc ttctcttaat ttatgattct tgaaatgcgc ctgctttaag
     3601 gctaaactag acgtatcaaa gttaagagtt tgtttttgta actggaaaga gatgaacaaa
     3661 tcaaatacat aaaagataaa ctcattttta ttcgtttggt aaaaataaaa tgcatatttt
     3721 taaagaaaga ttgatgaaaa atgtagattt gatgttttta cttgaataaa aagcggtttc
     3781 atagtaaaat aagaactgag aatgatggag gtaaatcggt ggaaaatctg aagaaaatgg
     3841 caggtatcaa ggctgctgag ttcgtgagtg atggaatggt cgttggactt ggaacgggct
     3901 cgactgccta ttattttgtc gaagaaatcg gtcgtcgtat caaggaagaa ggattgcaga
     3961 ttacagctgt aacaacttct agtgtgacca ctaaacaggc agaaggcctt aatatcccgc
     4021 tcaagtctat tgaccaagta gactttgtcg atgtgacggt tgatggcgcg gatgaagtgg
     4081 atagtcagtt taatggaatt aaaggcggtg gtggtgccct tctcatggaa aaggtggtcg
     4141 caacaccatc aaaagaatac atttgggtgg tggatgaaag caagctggtc gaaaaactag
     4201 gtgcttttaa attgccagta gaagtggttc agtatggtgc agagcaggtc tttcgtcatt
     4261 ttgaacgagc tggctacaaa ccaagtttcc gtgaaaaaga cggccaacgt tttgtgacgg
     4321 atatgcagaa ttttatcatt gacctcgcct tggatgtcat tgaaaatcca attgcttttg
     4381 gacaagaatt ggaccatgtc gttggtgttg tggagcatgg tttattcaac caaatggtgg
     4441 ataaggtcat cgttgctgga cgggatggag ttcagatttc aacttcaaaa aaaggaaaat
     4501 agaagggggc ataagatgtc taaatttaat cgtattcatt tggtggtact ggattctgta
     4561 ggaatcggtg cagcaccaga tgctaataac tttgtcaatg caggggttcc agatggagct
     4621 tctgacacac tgggacacat ttcaaaaaca gttggtttga atgtcccaaa catggctaaa
     4681 ataggtcttg gaaatattcc tcgtgaaact cctcttaaga ctgtagcagc tgaaagcaat
     4741 ccaactggat atgcaacaaa attagaggaa gtatctcttg gtaaggatac tatgactgga
     4801 cactgggaaa tcatgggact caacattact gagcctttcg atactttctg gaacggattc
     4861 ccagaagaaa tcctgacaaa aatcgaagaa ttctcaggac gcaaggttat tcgtgaagcc
     4921 aacaaacctt attcaggaac ggctgttatc gatgattttg gaccacgtca gatggaaact
     4981 ggagagttga ttatctatac ttcagctgac cctgttttgc agattgctgc ccacgaagac
     5041 attattcctt tggatgaatt gtaccgtatc tgtgaatacg ctcgttcgat tacccttgag
     5101 cgtcctgccc ttcttggtcg catcattgct cgcccttatg taggtgaacc aggtaacttc
     5161 actcgtacgg caaaccgtcg tgacttggct gtatctccat tttccccaac tgttttggat
     5221 aaattgaatg aggctggtat cgatacttat gctgtgggta aaatcaacga tatctttaac
     5281 ggtgctggta tcaaccatga catgggtcac aacaagtcaa atagtcatgg aattgataca
     5341 ctattgaaga ctatgggact tgctgagttt gaaaaaggat tctcattcac aaacctagtt
     5401 gactttgatg ccctttacgg ccatcgtcgt aatgctcacg gttaccgtga ttgcttgcat
     5461 gagtttgatg aacgcttacc tgaaattatc gcagctatga gagagaatga ccttctcttg
     5521 attactgcgg accatggaaa tgacccaacg tatgcaggaa cggatcacac tcgggaatat
     5581 attccattgt tggcctatag ccctgccttt aaaggaaatg gtctcattcc agtaggacat
     5641 tttgcagata tttcagcgac tgttgccgat aactttggtg tggaaactgc tatgattggg
     5701 gaaagtttct tagataaatt ggtataagat gacgcgctat gctttgctgg tgagaggtat
     5761 caatgttggt ggtaagaata aggtcgtcat ggcggagctt cgtcaagaat tgacaaactt
     5821 gggactggaa aaggttgaga gctacatcaa cagtggcaat attttctttg cttcgataga
     5881 ttccaaagcc caattggttg aaaagctaga gactttcttt gcagtccatt atccatttat
     5941 tcagagcttt tctttactga gtctagagga ctttgaggcg gaacttaaaa atctaccagc
     6001 ttggtggagc agagacttgg cacgaaaaga ttttctcttt tacactgagg gtttggatgt
     6061 ggaccaagtc atcgcgacag ttgaaagttt agagctgaaa gatgaagtgc tttattttgg
     6121 aaaacttggg attttctggg ggaaattttc tgaagaatcc tattctaaga ctgcctatca
     6181 gaagtacttg ctgaaggtgc ctttctaccg ccacattact attcgtaatg ctaaaacctt
     6241 tgacaaaatt ggtcaaatgc taaaaaaata ataaaggaga cacacaatga catttttaaa
     6301 caaaatccat gaaactgcta ctttcctgaa agaaaaggga attgcagccc ctgagttcgg
     6361 tctaatcctt gggtcaggac ttggagaatt ggcagaagaa atcgaaaatc cagttgtagt
     6421 agactatgct gagattccaa actggggccg ttcaacagta gtcggtcatg ctggtaaatt
     6481 ggtatatggt gaactggcag gtcgcaaggt cttggctctt caaggtcgtt tccatttcta
     6541 tgaagggaat cctctggaag tggtgacttt cccagttcgt gtgatgaaag ttcttggatg
     6601 tgaaggtgtt attgtaacca atgcagctgg cggtatcaga tttggtcctg gtaccttgat
     6661 ggctatctca gaccatatca acatgacggg gcaaaatcca ttgatgggtg aaaacttgga
     6721 tgactttggc ccacgtttcc cagatatgtc tagggcctac acaccagaat accgtgccac
     6781 tgcccatgaa gtggctaaaa aacttaatat caagcttgat gaaggtgtct atatcggagt
     6841 tactggtccg acttatgaaa caccagcaga aattcgttcc tataagacac tgggagcaga
     6901 tgcagttggt atgtctacgg ttcctgaagt tatcgtggca gcccactctg gcttgaaagt
     6961 tctgggaatt tcatgtatca ctaactttgc ggctggtttc caagaagaac tcaatcacga
     7021 agaagttgta gaagtgactg aacgtgttaa aggtgatttc aaaggcttgc ttaaagcgat
     7081 tcttgctgaa ttgtaagaaa aaagatttaa aaggggtagt gtctctgtct tttcaggatt
     7141 gactgcctat ccgtattaaa gaagaaacag aggaatacta tgagcttctt cctgctctta
     7201 taactgaaag aagcggaaga ataggtatgt ctgatctgat agccagcatt gtgaaagaca
     7261 agattctagg atactagcat tagctttcta gccaagcaga ctagtatgat aaggagagat
     7321 gagaatgaat tgactttctg aatttctcag tcttatcata tatagcacaa tgagatttcg
     7381 cttgagtctg cttgtaaata aacgaaaaga aagataataa ataatgaaaa ttggtcaacg
     7441 aattatgcgc tttggcataa aaaattaagt atcggagttg tatctgttgt agtcggcttt
     7501 gatttctagc tccagctgga atttcagcca atgaagtaaa gcaagatgta acatctgaag
     7561 tggtaatagg tgtgctagat tctaaggagg aattgaaaga gtcagaaaat gatgctccaa
     7621 aactagaaac tcctcttaga gaggagccaa gactagctcc tcaaacgctt ccggaagcaa
     7681 gtgaagttct tgaaaacaaa agggaagagt caaaagtaga gataacagag ccagctcaag
     7741 cggatgatat ccgcaaggtt gttggggaat tagccaagga tataagtatt actaagttgt
     7801 atatgacagg tcattctctt ggatgttacc tagctcagat tgcagcggtt gaagattacc
     7861 aaaaatatct tgatttttat aaccatgtat tgaggaaagt gacaactttc agtgctccta
     7921 aagtgattac ttccagaact gtttgggatg ctaagaatgg tttctgggat gttggtttgg
     7981 aaagtcgtaa attagctgtt agtggaaaaa ttaagcatta tgtggttgat aatgacaatg
     8041 ttgtgactcc cttgattcat aataatcgtg atattgttac atttacaggt aattcacgct
     8101 ttaaacaccg ttctcgtggc tattttgaaa gtccaatgaa tgatattcct aactttaata
     8161 ttggtaaaca agctaccttg gataaacatg gttatcgtga tccgaaattg gataaagtgc
     8221 gattctttaa gaaacaggct ctgcctcgat cttctagtca accaagcgct gaaccaatgg
     8281 aaaatattgc cttaggaaaa caggttactc aaagttcgac agctttcgga ggagatgcta
     8341 gaagagctgt ggatggcaaa gtcgatggta actatggtca caattctgtc actcatacaa
     8401 acttccaatc taagccttgg tggcaagtag atttggctaa agaagaaacc attcgccaaa
     8461 tcaatattta caactgaaca gacactgccc aggatagatt ggcaaacttt gatgtcattc
     8521 ttttagacag ttctggtaaa gaaattgagt gaaaacgtat aacatctcct aaagatgtgt
     8581 cagcacaaat tacgattaac cataaaaaag cgcgctatgt tcggattgag ctagaaggct
     8641 ataatgccct cagtcttgca gaagttgaag ttttccgctt tatagctacg aatggtgaaa
     8701 cggcgataca agtttctaag ccagttcaac caatcagtca gactcctgtg aaggataaaa
     8761 cattgacaat tcaacacagt ggagcttaca ttgcccgcta ctccataact tgggaagaag
     8821 ttccagtaga taaagatgga aaccaagttg ttcgtagtca ttcttgggaa ggaaacggtc
     8881 gcaaccagac tgcaggtttt gtcctcaacc tcccaatcaa agaaaatatg agaaatctgc
     8941 gagttaagat tgagaaaaag acgggcctac tatggaatag atggcaaaca atctatgaaa
     9001 acagaccaat tttagctcaa ccccaccgta aaattaccca ttggggtacg acattgaatt
     9061 ccaaggtgag tgacgatgat gtcttgtaat ctgatggtag aatgacagtt agtttgtcta
     9121 gtttataaga aagtactacc tgagcttgaa tagaactcag gtagctctct atgaaagaac
     9181 aaaattaata ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggttgcttaa
     9241 agcactgctt tgaggttgta gataagactg acgaagtcag tcacatatat aatccaaggc
     9301 gacgttgacg tggtttgaag agattttcga agagtataaa cagaaaggta gagcgcgtgt
     9361 tctaatttga acacgagtag aaaacttttc taaaaacaaa aacgaaagga tgggtaaact
     9421 gtattcgctg aactgaatac gggcgactct cctctaaatc aaaattaaga aagaaaggaa
     9481 ttgaacccac cctaaaagca gtgggaaaaa gatagttggt ctagcgagca tcgctcactg
     9541 cgcccaactc ctattttccc ttcgcttttt gatgggtttg gtatctttct caatataaaa
     9601 tataaaataa agaaaggtag agcgtgtgtt ttgatttgaa cacgagcgga aaactcggaa
     9661 aatagataat ctgactgaaa aatcaggatt tctcgtcagg ttcctaattt tcagtcgttt
     9721 tcttctcgct ctttgtatca taaattatgt ctatccatat tgctgctcag cagggtgaaa
     9781 ttgctgataa aattcttctt cctggggatc ctcttcgtgc taagtttatt gcggagaatt
     9841 tccttgatga tgctgtttgt tttaacgaag tgcgtaacat gtttggttac actggtactt
     9901 acaagggtca ctgtgtatct gtcatgggaa ctgggatggg aatgccatct atttcgattt
     9961 atgcgcgtga gttaatcgta gactacggtg tgaagaaatt gattcgtgtg ggaactgcag
    10021 gttctttgaa tgaagaggtt catgttcgtg aattagtttt ggcgcaggcg gctgcaacca
    10081 actcaaacat cgttcgtaat gactggccac agtacgattt tccacaaatt gctagctttg
    10141 atttgcttga taaagcctac catatcgcca aaaaacttgg tatgactact cacgttggga
    10201 acgttttgtc atctgatgtc ttttactcaa attactttga aaagaatatc gagcttggta
    10261 aatggggagt caaggctgtg gaaatggaag cagcagctct ttactatctt gctgcccaat
    10321 accatgttga tgcgctagct atcatgacca tctctgatag cttggtcaat ccagacgaag
    10381 acacaactgc agaagaacgt caaaatacct tcactgatat gatgaaggtt ggtttggaaa
    10441 ccttgattgc agaataatta tagccaaaaa ggaagccctt tatgaattgt aggacttcct
    10501 tttcttatcc agaaattgat ctagctctct ctgatttcga aggatagtga ctttatgtga
    10561 atattcttgg caaagttttt ggtaattttc tttttgagtt ttgctacgcc catcccaaag
    10621 aatccatctg ataaactccc actcaaagcg ttcagggcaa tctgccgcca tacggcaatc
    10681 tgccgccata ctttctctga cttttccacg gtatttaaga taacgcttaa aggctctaaa
    10741 gagacaggtc aatggcaaaa aattgagaaa gatgatttgg tcagcttctt gcattcgttc
    10801 ttggtagtag caccaagaat aattaccatc gatgacccaa gctttatgct tggtgagaaa
    10861 gttttttatc tcggttaaca tccattcgca gtcactgtct tgccaaccag gttgaaattg
    10921 gagtgtgtcc atgtgcagtt ttggaatgga gtagtagtta gataactttt ctgctagagt
    10981 tgacttacca gaaccagaat atccgataat tgtgattttc attttctacc ttttcctatt
    11041 tggagacaaa aaaacagcct ctatggactg tttcttattt agcaagttta gctgaaagac
    11101 gagctttatc gcggcttgct ttgtttttgt gaatcaaacc tttagtttct gctttatcga
    11161 tagctgagct agcagcacgg aaaagttctt cagatgggtt tgcttcgaaa gcttttatag
    11221 cagtacgcat agctgatttt tgagctgagt tcttttcgtt ttgtttaacg ttcaattcag
    11281 cgcgtttgat agctgattta atgtttgcca atgttcttac ctccatattt actaactata
    11341 ccattatata tgaaaactta cgttttgaca agggaaaatt atttttttaa gtaaatttca
    11401 tcgatttcat ggttcttgct tttatgaaga atcacttctg cacgatttct ggttggttca
    11461 atataatttt gtagatttgt gagattgata ctggtccaga cctgatgggc aaaggcttcc
    11521 acttccccaa tcggcatctg agtaaaacga taatagtagc tatcagggtc gttttgggct
    11581 agactcagca ttttcaagaa acggtccaga taccaacttt caatatcatc cactccagca
    11641 tctacataga tggaaaagtc aaagaagtca gtgatataga gacgatcgtt ttgtggattt
    11701 tgaaagacat tgattccctc aacaattaca aaatcagcag ctttgacact ttgtttctct
    11761 tcgggtacga tgtcgtaaac ttcatgagaa tagacaggaa tatctacatc ttgtccattt
    11821 ttgatgcggt ccaagaagtt gagaagagct tccatatcat agctttcagg aaatccttta
    11881 cgatttaaaa tcccctgctc aatcaaggtt tgattgggat agagaaaacc atcagttgta
    11941 accaactcaa ccgtagcatc tgtaaacgta cgggacagta ggatttgaag taggcgactg
    12001 gttgtggatt ttccaacggc aacactccca gaaaccccaa taataaaagg ttgagattta
    12061 ctttcacgtt ggaggaaaat tccttttgaa aaggctaaat cttccttagt tcgcttgtaa
    12121 atctgaatca gatgagccaa ggggagatag atatctgtaa cgtcttggag actgatttgg
    12181 tcattaaaac tcttgataga ttccaattct tcttctgtca aaggaggtgt tgtctttcga
    12241 tgtaaagatt gccaagtctg gcggctgatt ttttcaaaat gtaaaaattc gttggtcatt
    12301 tgttttcccc tagatatttt gtctatcata ccataaaaat ctaaaaaaat aaagcggttt
    12361 cttgaatgtg gtaagtcaat ggtatatagt ataagtgatc aatttattga tgaaatagga
    12421 gatagattct actgatttat aagtttataa gcttataaag ggggcttttt agttcacaac
    12481 ttataggagg tgtactatga aaatcttaaa acgttacata ttggaactct gttttatttt
    12541 aagttttgct ttacctttta taaaaggagc caatgcagat aatggtagat gctttgtgga
    12601 aacctattac ggttttactt ttttgatgga acatgctatt gtaacagctg tctttatcta
    12661 ttcgttttta attgctttct tactaaaaaa acgatgggcg aaatggattg ctgctggtag
    12721 ttattgcttt ttagtcctat ggattgctgc tggtagttat tgctttttag tcctatggat
    12781 tgcgacagag ggttatttct tccgcatgtc actagaagat ttgatacgac tttggacaag
    12841 tttggaaatc ctgacacaaa cgtatcagtt gggcttttat ctaaacattt tgttgggaat
    12901 tttgttgata ataaagtatt ttaaggttaa gcaatagaaa taataatctg cctgattata
    12961 gatgataaac tttttgtttt tatctggact gtgatattta tagttctaaa tggtttatta
    13021 tttataagtg ttgaaagaga ttgaagaaaa tggcttcgtg actcgctttc ttcggtcttt
    13081 ttcgttttat tatcaatttg taaacgattt acaatttgag tcaaattatg gtaaaatagt
    13141 gtcatgagta aaatgtatta tgcagaaaat cctgacgctg ctcacgacat tcatgagttg
    13201 agagtagagt tgttgggaca aaaaatgacc tttttaacgg atgcgggtgt ttttagcaag
    13261 aaaatagttg actttggaag tcaactattg ctcaagtgtc ttgaggtcaa ccaaggagaa
    13321 actgtccttg atgtaggctg tggttacgga cctttgggtt tgtccttagc taaggcttat
    13381 ggagttcagg ctactatggt tgatatcaac actcgtgcct tagatttagc tcggagaaat
    13441 gctgaaaaaa ataatgcaaa agcgacgatt ttccagtcca acatctatga acaagttgaa
    13501 ggacattttg atcatgtcat ttccaatcct cctatccgtg cgggcaagca agtcgttcat
    13561 gaaatcattg agaaaagtaa agatttcttg gaaactagtg gggatttgac aatcgtcatc
    13621 cagaaaaaac aaggggctcc aagtgctaag tccaagatgg agggtgtgtt tggtaattgt
    13681 gaaatcctta aaaaagacaa gggatactat atccttagaa gtgtgaaaga atgagagcag
    13741 ttgatttaat acaaaaaaaa cgagacggtc aagaactgac ttcaagtgaa attgaatggc
    13801 tggtagaagg ctatgtgtca ggaactgttc ctgattatca gatgtctgcc tttgctatgg
    13861 ctgtttattt taaaggaatg acgactcgag aaatctctga tttaacgatg aatatggtta
    13921 agactgggca agagtttgac ctgtcagcca ttgatggtgt gaaagttgac aagcattcta
    13981 ctggtggtgt cggcgataag gtaaccttga ttttagttcc tcttgttgct agcttcggtg
    14041 tgcctgtagc aaaaatgagt ggtcgtggtc ttggccatac aggcggaaca attgataaat
    14101 tggagtccat taagggctat caagtagaac gtagtcaaga ggattttatt cgtcaggttc
    14161 aggatattgg agtatctgtc attgggcaat cagaccagct ggtcaaagca gataagcttc
    14221 tctatgccct tcgtgatgtg accgcaactg tcgacacgat tcctttgatt gcgagttcgg
    14281 tgatgagcaa gaaaattgcg gcaggggcgg atgctatttt gctagacgtg actgtcggtg
    14341 agggtgcctt catgaagacg gttgatgagg cgcgtgagtt ggctcaaact atggtagatc
    14401 ttggtaaggt tgttggacgg aagacagtag cagtgattac ggatatgagc cagcctttgg
    14461 gacgagcgat tggaaatcgt ctggaaatcc ttgaagcatt ggagattttg caaggtcaag
    14521 gacgtcagga tattacccac tttatttgtg aattggctca aattatgctt ggtttggcaa
    14581 atgttaacaa aactgttgaa gaagttcgcc aacatcttga gaatggtcaa gcactggcta
    14641 agtttgagga aatggtgcaa gcccaaggtg gagacttgga agacctctat cgtcctgtaa
    14701 atgtagctca tgtggtggaa atccctgctc aggaaacggg tgtcatttca gctcttccag
    14761 ctatggactt tggtctttat gccatgaggc tgggtgctgg tcgtgcagtt aagtctgatg
    14821 ccttggacta tgaaacagga attgtttttg aaaagaagat tggagactcc gttcaaaagg
    14881 gtgaaattgt tgcaaaagta tacacaaatg aaaaaattcc acctcaacta gttacagatt
    14941 tccaaaaatg tgttaaaata agtgatgaag taaaaaaaat ccgtgaaatt gtagaaatca
    15001 tctcctaaat ggagggaaaa ccgaaatgaa attaaataaa tatatagatc atacgctttt
    15061 aaaacaagat gcaaagaaaa aacaaattga tagtttgttg tctgaggcta gagagtatgg
    15121 ctttgccagt gtttgcgtta atccgacctg ggttgaacat gctaaaaaag gacttgaagg
    15181 cacagatgtt aaggtttgca cagtagtagg tttccctttg ggagcaacaa cttcagccgt
    15241 gaaagcattt gagacaaaag aagctatcca aaatggtgca gatgagattg atatggtgat
    15301 caatgttgga gctctcaaat caggtaattt agccttggtt gagtcagata ttcgcgcagt
    15361 agtggaagca agtggtgata agttagtgaa agtcattatt gaagcttgcc ttctgacaga
    15421 ccaagaaaaa attgttgttt gccaattggc ccaaaaagct ggggctgact ttgttaaaac
    15481 atctactggt ttttcaactg gtggtgctac gatagcagat gttacattaa tgcgtgaaac
    15541 agttggatct gatatgggag tcaaggctgc tggtggagct cgttcttatg cagatgctct
    15601 tgcctttgtc gaagcaggtg cgacccgtat cggaacgtca gctggggtag ctattttaaa
    15661 aggagaattg gcagatggcg actactgagt tgattgaact ggcaattgaa accagcaaac
    15721 atgcctatgt cccctattct cactttccta tcggagcagt tttagtagcc aaagacggaa
    15781 gtgtttacac gggagtgaat atcgaaaatg ctagctatcc tttgaccaat tgcggtgaga
    15841 gaacagctat ttttaaggct atatctgaag gccaaagaga attttcagaa ttgattgtct
    15901 atggtcaaac tgaaaaaccg atctcgccat gtggtgcttg tcgccaagtg atggctgaat
    15961 tttttgaaca agatctaaaa gtgaccttag tcgcaaaaga taaatcgacg gtcgagatga
    16021 cggtcgggga attacttcca tactctttta cagacttaaa ctagtctgag tcgctctctg
    16081 agtggcacgg tccttgtggc caaccaatcc atacttgcaa catcgttgca catcttattt
    16141 aggaggttca gtaatgaaca agaaacaatg gctaggtctt ggcctagttg cagtggcagc
    16201 agttggactt gctgcatgtg gtaaccgctc ttctcgtaac gcagcttcat cttctgatgt
    16261 gaagacaaaa gcagcaatcg tcactgatac tggtggtgtt gatgacaaat cattcaacca
    16321 atcagcttgg gaaggtttgc aggcttgggg taaagaacac aatctttcaa aagataacgg
    16381 tttcacttac ttccaatcaa caagtgaagc tgactacgct aacaacttgc aacaagcggc
    16441 tggaagttac aacctaatct ttggtgttgg ttttgccctt cataatgcag ttgaagaagc
    16501 agctaaagaa cacactgact tgaactatgt cttgattgat gatgtgatta aagaccaaaa
    16561 gaatgttgcg agcgtaactt tcgctgataa tgagtcaggt taccttgcag gtgtggctgc
    16621 agcaaaaaca actaagacaa aacaagttgg ttttgtaggt ggtatcgaat ctgaagttat
    16681 ctctcgtttt gaagcaggat tcaaggctgg tgttgcgtca gtagaccctt ctatcaaagt
    16741 ccaagttgac tacgctggtt catttggtga tgcagctaaa ggtaaaacaa ttgcagccgc
    16801 acaatacgca gccggtgcag atattgttta ccaagtagct ggtggtacag gtgcaggtgt
    16861 ctttgcagag gcaaaatctc tcaacgaaag ccgtcctgaa aatgaaaaag tttgggttat
    16921 cggtgttgac cgtgaccaag aagcagaagg taaatacact tctaaagatg gcaaagaatc
    16981 aaactttgtt cttgtatcta ctttgaaaca agttggtaca actgtaaaag atatttctaa
    17041 caaggcagaa aaaggagaat tccctggcgg tcaagtgatc gtttactcat tgaaggataa
    17101 aggggttgac ttggcagtaa caaacctttc agaagaaggt aaaaaagctg tcgaagatgc
    17161 aaaagctaaa atccttgatg gaagcgtaaa agttcctgaa aaataatgga tggaagtcat
    17221 ttcttaggaa gcggccaagg gccgcttttt taagagttga gacaaagtca ttttgtctca
    17281 gtaaagcttg ctactagaca aactagcaag ttttattgaa ataaaataag actctgaaag
    17341 gaagagcaca tggcacacga aaatgtcatt gagatgcgtg atattaccaa ggtgtttggt
    17401 ggatttgttg ccaacgacaa aatcaacttg cacctacgaa aaggtgaaat tcatgcactt
    17461 ttaggagaaa atggggctgg taagtccacg ctaatgaaca tgttagcagg ccttcttgaa
    17521 ccaactagtg gtgaaatcgc ggtcaacggt caagttgtca atctcgactc cccatctaaa
    17581 gcagctagct tgggaatcgg gatggttcac cagcacttta tgttggttga agccttcaca
    17641 gtggctgaaa acatcatttt aggtagtgaa ttgactaaaa atggtgtgct agatatcgct
    17701 ggagctagca aagaaatcaa ggctctttct gaacgttatg gcttagctgt tgacccttct
    17761 gccaaggtag cagatatctc agttggagcc caacaacgtg tagaaatttt aaaaacactt
    17821 tatcgggggg ctgatatcct tatctttgac gaaccaacgg ctgttttgac tccatcagaa
    17881 attgatgagt tgatggctat tatgaaaaat cttgtcaaag aaggaaaatc aattatcttg
    17941 attacccaca aattggatga aattcgcgca gtttctgacc gtgttacagt tatccgtcgt
    18001 gggaaatcaa ttgaaaccgt tgaaattgca ggggctacca atgctgattt ggcggaaatg
    18061 atggtaggac gttctgtttc ctttaaaaca gagaagcaag cctctaaacc aaaagaagtg
    18121 gttttgtcta tcaaagattt ggtggtcaat gaaaaccgtg gtgttccagc tgttaaaaat
    18181 ctgtccttgg atgttcgtgc tggagagatt gttggtattg cggggattga tggaaatggt
    18241 cagtctgaac tgattcaagc cattacaggt cttcgtaagg ttgaatctgg tagcattgag
    18301 ctaaaaggag attcaattgt aggcttgcac ccacgtcaga ttacagaact aagtgttggg
    18361 cacgttccag aagaccgtca ccgtgatggc ttgattttgg aaatgatgat atctgaaaat
    18421 attgcccttc aaacctacta taaagaacca catagtaaaa atggaatttt gaattattca
    18481 aatattactt cttatgctaa aaagctgatg gaagagtttg atgttcgcgc tgccagtgaa
    18541 ttagttcctg cagctgcact ctcaggagga aatcaacaaa aagcaattat tgctcgtgaa
    18601 attgatcgag atcctgatct ccttatcgtt agccagccaa ctcgtgggtt ggatgtcggt
    18661 gccattgagt atatccacaa acgcttgatt gaagagcgtg ataatggcaa ggctgtcctt
    18721 gttgtcagct ttgaattgga tgagatttta aacgtctcag accgtattgc cgttatccac
    18781 gatggtaaga ttcaaggtat tgtatcacca gaaacaacca ataaacaagg acttggtgtc
    18841 ttgatggctg gtggaaactt gggaaaggag aagagtgatg tctaaaaaat tacaacaaat
    18901 ttcggttccc ttgatttctg tattcctagg aattttactc ggagccattg tcatgtggat
    18961 cttcggttat gatgctattt ggggctacga agaattgttc tatacagcct ttggcagtct
    19021 gcgtgggatt ggagaaatct tccgtgctat gggtcctctg gtcttgattg gtcttggttt
    19081 tgcggttgcc agtcgagctg gtttctttaa cgtcggactt cctggtcagg ctttggcagg
    19141 ttggattctc agtggttggt ttgccctgtc gcatccagat atgccccgtc ccttgatgat
    19201 tctagcaacc atcgtgattg ccttgattgc tggtgggatt gtcggagcga ttccaggtat
    19261 tcttagggcc tatctaggga cgtcagaggt tattgtaacc atcatgatga actacattgt
    19321 cttgtatgta gggaatgcct ttatccatgc tttccctaaa gacttcatgc aaagtacaga
    19381 ttcgaccatt cgtgttgggg ctaatgcaac ctatcagaca ccttggttgg ctgagttgac
    19441 tggtaactca cggatgaata ttggtatttt ctttgccatc attgccgttg cagttatttg
    19501 gttcatgctc aagaaaacaa ctcttggttt tgaaatccgt gcagttggtc ttaatccaca
    19561 tgcttcagaa tatgctggta tttctgccaa gcggactatt atcctatcta tgattatttc
    19621 aggtgccttg gcaggtcttg gtggagctgt tgaaggtttg ggaaccttcc agaacgtcta
    19681 tgttcaaggt tcgtcattag ctatcggatt taacggaatg gcggttagtt tgcttgcggc
    19741 caactcacca attggtatac tctttgcagc cttcctattt ggcgttctcc aagttggggc
    19801 tcctggtatg aatgcggcgc aggtaccatc tgagcttgtc agcattgtaa cagcgtctat
    19861 tatcttcttt gtcagtgttc attaccttat cgaacgcttt gtcaaaccga aaaaacaagt
    19921 taaaggaggt aagtaaagat gtctattata accttgctcc ccctcttggt gtcttctatg
    19981 ctgatttact cagcacctct catctttaca agtatcggtg gtgttttctc tgaacgtggt
    20041 ggtgtggtaa acgttggcct tgaaggaatt atggttatgg gtgccttttc tggagttgtc
    20101 tttaaccttg aatttgcaga acaatttgga gcagcaactc catggctatc cttgcttgta
    20161 gcaggattgg ttggtagtgt tttctctatc atccacgcag cagcgacggt tcatttccgt
    20221 gcagaccatg ttgtcagcgg tacggtattg aacttgatgg cgcctgcctt ggctgttttc
    20281 ttagttaaag ttctttataa caaaggacaa accgacaacc taagtcaaac ttttggacgc
    20341 tttgatttcc cagtcttggc aaatatccca gtgattggtg atatcttctt caagtcaact
    20401 agtctacttg gttatctggc gattgccttc tcattccttg cttggtttat tctctttaag
    20461 actcaatttg gtcttcgtct ccgctctgtc ggtgaacatc ctcaagcagc ggataccttg
    20521 ggaatcaacg tctacaagat gagatattta gggattatta tttcaggttt tctaggtgga
    20581 attggcggag cgatttatgc gcaatcaatc tcagttaact tctcagtgac aactattgtt
    20641 ggacctggat ttatcgccct tgctgcgatg atctttggga aatggaatcc aatcggtgct
    20701 atgctttcta gtctcttctt tggactttca caaagtttgg ctgttatcgg ttctcaattg
    20761 ccgttcctac aaggagtgcc agcggtttat cttcaaattg caccttatgt tttgacaatt
    20821 cttgtcttag cagccttctt tggaaaagca gtcgcaccaa aagcagatgg tatcaactac
    20881 atcaaatcaa aataagcata caaaaaaacg tcagtccaat gactgaactg ccccccaaaa
    20941 gttagacaga aaaaatctaa cttttggggt gtttttatta tgaaattaag ttatgatgat
    21001 aaagttcaga tctatgaact aagaaagcaa ggatatagct tagagaagct ttcaaataaa
    21061 tttgggataa acaattctaa tcttaggtac atgattaaat tgattgatcg ttacggaata
    21121 gagttcggca aaaaaggaaa aaatcgttac tattctcctg atttaaaaca agaaatgatt
    21181 cataaagtct gacatgaagg ctggactaaa gatagagttt ctcttgaata cggtctccca
    21241 agtcgtacga tacttcttaa ctggctagca caatacagga aaaacgggta tactattgtt
    21301 gagaaaccaa gagggagagt acctaaaatg ggacgtaatc caaaaacgag acctgaagag
    21361 aggacagaat tagaacgtct tcaagcagaa aatgagtacc tgagagcgga gaatgccatc
    21421 ctaaaaaagt taagagaact ccgattgaag gaggaaaaag agaaagaaga aagacagaaa
    21481 ttgttcaaga attaatgact gagttttcgt tagatcttct tctaaaagcc attaaactag
    21541 ctcgttcgac ctactactat cacttgaaac agctagacaa aacagataag gaccaagagc
    21601 ttaaagctga aattcaatcc atctttatcg aacacaaggg aaattatgct tatcgtcgga
    21661 tttatttaga actaagaaat cgtggttatc tggtaaatca taaaagagtt caacacttga
    21721 tgaagtactc aatttacaag ctaaaacgcg acagaaacga aaatattctt ctcataaagg
    21781 agacgttggc aagaaagcag agaatctcat tcaaggccaa tttgaaggct ctaaaacaat
    21841 ggaacagtgc tacacagatg tgactgaatt tgccattcca gtaagtactc aaaagcttta
    21901 cttatcacca gttttagatg gctttaatag cgaaattatc gcctataatc tttcaacttc
    21961 acccaactta gaacaagtac aaacaatgtt ggaacaggca ttcacagaga agcactacga
    22021 gaatacgatt ctccatagtg accaaggctg gcaatatcaa cacgattctt atcatcggtt
    22081 cctagagagt aagggaattc aagcatctat gtcacgcaag ggaaacagcc aagacaacgg
    22141 tatgatggag tccttctttg ggattctgaa atccgaaatg ttttatggct atgagaaaac
    22201 atttaaatca cttaaccaat tggaacaagc cattatagac tatattgatt actacaacaa
    22261 caaacgaatt aaggtaaaac taaaaggact tagccctgtg caatacagaa ctaaatcctt
    22321 tggataaatt atttgtctaa ctgtttgggg gcagtacaga actggcgttt tattttttag
    22381 gatcttgatg ggttaggttc aatccccaag ggaccaaatt ttcagtttta tttttgatac
    22441 gagctatatt gtccttatga cgaatgataa tcaaactagc aagtgctaag ataatagcga
    22501 tgaagagaga gtcatagtta ctcaggataa aaccaaaaag tggaaagagc agaaccccga
    22561 taacagccgc gatcgatgct gtgacactag acagtgaaat catactgcca agatagagag
    22621 ctccaaagaa gataatcgca aggtagagac agaagatagg cgcaaatccg aaaatcactc
    22681 cagcactggt tgcgacagcc ttaccacctt taaatcctgc aaagataggg aaggtatggc
    22741 cgataacagc caaaagtcca aagatgagag gagaaacgcc ttgtagatga aaaataatcg
    22801 gaagcagcgt tgctagggtt cctttgaaaa agtcaatcac aaaggttgcc ataccagctt
    22861 tcttacctaa aatgcggaag gtgttggtcg ttccagtgtt accagaacca tgctcgcgta
    22921 gattgatttg aaagaatact tgtccaatcc agagaccaga tggaatcgaa cccagcagat
    22981 aggctaggat taataaaact attgtaatca tactcctatt atatcatgaa ttgggaaaga
    23041 aaagcagaga atctctgctg aaattgtcac atcggagaaa gaaaaatttt gcaaaatcct
    23101 tggaaaacct gtagaatagt aaagatgaac gaataggagg ttccttgtgt caaaaaagga
    23161 aatcaatatt aacaattata atgatgatgc tattcaggtg ctagaagggt tggatgcggt
    23221 ccgaaaacgt ccagggatgt atattggatc gaccgatggc gctggtcttc atcacctagt
    23281 ttgggaaatc gttgataatg cagtcgatga agccttgtct gggtttggtg atcgtattga
    23341 tgtaactatc aataaagacg gtagtctaac ggttcaagac catggacgtg ggatgccgac
    23401 aggtatgcac gctatgggaa ttccaactgt tgaggttatc tttaccattc ttcatgccgg
    23461 agggaaattc ggtcaaggtg gctataagac atcaggtgga cttcacggag tgggttcttc
    23521 cgttgttaac gccctttcta gctggttaga agttgaaatt acccgtgatg gcgcagttta
    23581 caagcaacgt tttgaaaatg gtggaaaacc tgtcacgact ttgaagaaaa tcggtacagc
    23641 actcaagtct aaaacaggca ccaaagttac ttttatgcct gacgcgacta tcttttctac
    23701 gacagatttc aagtacaata ccatttcaga gcgccttaat gaatcagcct ttctcttgaa
    23761 aaatgtgacc ttgtctttaa cggacaagcg aacagatgaa gcgattgagt tccactatga
    23821 gaatggagta caagattttg tttcttatct caacgaagat aaggaaatct tgatgccagt
    23881 tctttacttt gaaggggaag acaatggttt tcaagtggaa gtagccctcc agtacaatga
    23941 cggattctca gataacattc tatcctttgt caataacgtt cgcaccaagg acggtggaac
    24001 gcacgagaca ggactcaagt ctgccattac caaggtcatg aatgactatg cacgtaaaac
    24061 aggtcttctc aaggaaaaag ataaaaacct tgaaggttca gactatcgtg agggactagc
    24121 ggccgttctt tctatcttag ttcctgaaga acacttgcag tttgaaggac agaccaagga
    24181 taaactagga agccccctag ctcgcccagt tgtggatgga atagtggctg ataagttaac
    24241 ctttttcctt atggaaaatg gggaattagc ttctaacctc atccgcaagg ctatcaaggc
    24301 ccgtgatgct cgtgaagcag cacgtaaggc gcgtgatgag agccgaaatg ggaagaaaaa
    24361 caagaaagat aagggcttgt tgtctgggaa attgacccca gcccaatcta agaatcctgc
    24421 taagaatgaa ctctatctag ttgaggggga ctctgccggt ggttctgcca aacaaggtcg
    24481 tgaccgcaag ttccaggcta ttctacctct tcgtggtaag gttatcaata cagccaaggc
    24541 caagatggcg gatatcctca aaaatgaaga gatcaatacc atgatttata ccattggtgc
    24601 gggtgttgga gcagacttct ctattgaaga tgccaactat gataagatca ttatcatgac
    24661 cgatgcggat accgacggtg cccatatcca gaccttgctc ttgacatttt tctaccgtta
    24721 catgcgtccg ctagtcgagg caggtcatgt ctatattgcc ctcccacctc tttacaagat
    24781 gtccaaaggt aaaggcaaga aagaagaagt ggcctacgct tggacggacg gagaactaga
    24841 agaactccgt aaacagttcg gtaaaggcgc taccctccaa cgatacaaag gacttggtga
    24901 gatgaatgcg gaccagctct gggaaacaac catgaaccca gaaacacgta ccctcatccg
    24961 tgtcacaatt gaagatttag cgcgcgccga acgccgcgtc aatgttctca tgggagataa
    25021 ggtagaacca cgccgtaaat ggattgaaga taatgtcaag tttacgctag aagaagcgac
    25081 agtgttttaa tgaaagaaaa taatatgtta gtaaactgga aattagagtc ggatgtgaat
    25141 gactatgtga aaaagcagtt tgaaaatctt ggtttgaaaa aattgcagga ttattaatga
    25201 agaatctgct atgagtgctt atcttaaaga ggcgcttaag ggagcggtta agacacaaac
    25261 taagactaat tttggaaaac cagattttca tattgaaaaa tacaaacagg atagtttggt
    25321 tacttatcgt aactgaacac gggacgaatt cccgtaaaat atcttttact gcataaaaac
    25381 ctactctaca ttctttgaaa ggagttgaac acgccctaga tactgtgtga aaaagataaa
    25441 ttctcttgtg agtttgctta cttcaagaat tttctatttt cacttggtat tttatgggct
    25501 ttgtatctta tgtctaacat tcaaaacatg tccctggagg acatcatggg agagcgcttt
    25561 ggtcgctact ccaagtacat tattcaagac cgggctttgc cagatattcg tgatgggttg
    25621 aagccggttc aacgccgtat tctttattct atgaataagg atagcaatac ttttgacaag
    25681 agctaccgta agtcggccaa gtcagtcggg aacatcatgg ggaatttcca cccacacggg
    25741 gattcttcta tctatgatgc catggttcgt atgtcacaga actggaaaaa tcgtgagatt
    25801 ctagttgaaa tgcacggtaa taacggttct atggacggag atcctcctgc ggctatgcgt
    25861 tatactgagg cacgtttgtc tgaaattgca ggctaccttc ttcaggatat cgagaaaaag
    25921 acagttcctt ttgcatggaa ctttgacgat acggagaaag aaccaacggt cttgccagca
    25981 gcctttccaa acctcttggt caatggttcg actgggattt cggctggtta tgccacagac
    26041 attcctcccc ataatttagc tgaggtcata gatgctgcag tttacatgat tgaccaccca
    26101 actgcaaaga ttgataaact catggaattc ttacctggac cagacttccc tacaggggct
    26161 attattcagg gtcgtgatga aatcaagaaa gcctatgaga ctgggaaagg gcgcgtggtt
    26221 gttcgttcca agactgaaat tgaaaagcta aaaggtggta aggaacaaat cgttattact
    26281 gagattcctt atgaaatcaa taaggccaat ctagtcaaga aaatcgatga tgttcgtgtt
    26341 aataacaagg tagctgggat tgctgaggtt cgtgatgagt ctgaccgtga tggtcttcgt
    26401 atcgctattg aacttaagaa agacgctaat actgagcttg ttctcaacta cttatttaag
    26461 tacaccgacc tacaaatcaa ctacaacttt aatatggtgg cgattgacaa tttcacacct
    26521 cgtcaggttg ggattgttcc aatcctgtct agctacatcg ctcaccgtcg agaagtgatt
    26581 ttggcgcgtt cacgctttga caaagaaaag gctgagaaac gtctccgtat cgtcgaaggt
    26641 ttgattcgtg tgatttcgat tttggatgaa gtcattgctc ttatccgtgc ttctgagaat
    26701 aaggcggacg ccaaggaaaa cctcaaagtt agctatgatt ttacggaaga acaggctgag
    26761 gctatcgtaa ctttgcaact gtaccgtttg accaataccg atgtggttgt cttgcaggaa
    26821 gaagaagcag agcttcgtga gaagattgct atgctggcgg ctattatcgg tgatgaaagg
    26881 actatgtaca atctcatgaa gaaagaactt cgtgaggtca agaagaactt tgcaactcct
    26941 cgtttgagtt ctttagaaga cactgcgaaa gcaattgaga ttgatacagc tagtcttatc
    27001 gctgaggaag atacctacgt cagcgtgacc aaggcaggtt acatcaagcg taccagtcca
    27061 cgttcctttg cggcttccac cttggaagaa attggcaagc gtgatgatga ccgtttgatt
    27121 tttgttcaat ctgccaagac aacccagcac ctcttgatgt tcacaagtct tggaaatgtc
    27181 atctacagac caatccatga gttggcagat attcgttgga aggacatcgg agagcatctg
    27241 agccaaacca tcacaaactt tgaaacgaat gaagcaatcc tttatgtgga agtactggat
    27301 cagtttgacg atgcgacaac ctactttgca gcgactcgcc ttggtcaaat caaacgggta
    27361 gagcgaaaag aattcactcc atggcggacc tatagatcta agtctgtcaa gtatgctaag
    27421 ctcaaagacg atacagatca gattgtagca gtggctccga ttaaactaga tgatgttgtc
    27481 ttggttagtc aaaatggtta tgccctgcgt ttcaatatcg aagaggttcc ggttgtcggt
    27541 gctaaggcag caggtgtcaa ggctatgaat ttgaaagaag atgatgtcct ccaatctggc
    27601 tttatctgta atacttcgtc cttctacctc ttgacccagc gtggaagctt gaaacgtgtt
    27661 tccattgagg aaattctagc aaccagccgt gccaaacgag gattacaagt cttgcgtgag
    27721 ttgaaaaaca aaccgcatcg tgtcttcttg gcaggagcag ttgcagagca aggatttgtt
    27781 ggcgatttct tcagtacgga agtggatgtg aacgaccaaa ctctgcttgt ccaatccaat
    27841 aaaggaacaa tctatgaaag ccgattgcaa gacttgaact tgtcagaacg cactagcaat
    27901 ggaagcttca tttctgacac gatttcagat gaagaagttt ttgacgctta tcttcaggaa
    27961 gtagttactg aagataaata agtgaaatga agaatcagtt ctaagatggt ttttattgga
    28021 ggaattttct gaaaattaca aaatatactt gctattttag aaaaatagtg tagaatataa
    28081 gaaataggtt tttagaataa ggagtggaat atgacagtaa cgattgattg ggaaaacctc
    28141 ggtttttcct atatgaaatt accttatcgc tatcttgctc atttcaaaaa tggacaatgg
    28201 gatcaaggag agcttacaga ggatgcaact ttgcatattt cagagtcttc tccaagtctt
    28261 cactatggac aacaagcatt tgaaggtttg aaagcttatc gtactaagga tggcagtgtt
    28321 caactgttcc gtcctgatga aaatgctaaa cgcctgcaac gtacatgtga ccgtctcttg
    28381 atgccacaag ttccgacaga catgtttgta gaagcttgta aagcagttgt ccgtgcgaat
    28441 gaagaatacg taccaccata cggaataggt ggaaccttat atcttcgccc tcttttgatt
    28501 ggtgtcggag atattatcgg ggtaaaaccg gcagaagagt acattttcac catctttgct
    28561 atgccagttg gaaattactt taaaggtggt ttggtcccaa ccaacttctt gattcaggat
    28621 gagtacgacc gtgcagcacc aaatggtaca ggtgcggcta aggttggtgg aaactatgct
    28681 gcaagtctct taccaggaaa aatggccaag tcacgccatt tctcagatgt tatctatctg
    28741 gacccatcaa ctcatacaaa gattgaagaa gtcggatcag ctaatttctt tggaattaca
    28801 gctgataatg aatttgtaac accattgagt ccatctatct tgccatctat taccaagtat
    28861 tccttgcttt atttggcaga acatcgcttg ggattaactc ctattgaggg tgatgttcca
    28921 attgataatc ttgaccgttt tgtagaggca ggtgcctgtg gtacagcagc ggttatttct
    28981 ccaattggag gtattcaaca tggtgatgat ttccatgtat tctatagtga aacagaagta
    29041 ggtcctgtga cgcgtaaatt atataatgaa ttgacgggta ttcagtttgg cgatattgaa
    29101 gcgccagaag gttggattgt aaaagtagat taaaataaac caaaggagat tttttatgaa
    29161 atagaaaaag tggctcttaa cagcaggagt ggtcctgagc acgttagcta ttttagtggc
    29221 ttgtggaaaa actgataaag aagcagatgc accgacaaca ttttcttatg tctatgcagt
    29281 agatccagca tcattgggct acagtataac gactcgaaca tcgacgacag acgttattgg
    29341 aaatgttatt gatggtttga tggaaaatga taaatacggc aatgttgctc cttctcaaaa
    29401 agactatgat ttgaatagta caggatgggc tccaagctat caagatccag cgtcttactt
    29461 gaatattatg gatccaaaat ctggttctgc catgaaacac cttggtatta cgaaaggaaa
    29521 agataaggat gttgtagcta aacctggttt ggataaatat aagaaattgt tagaagatgc
    29581 tgtttctgag accactgacc tagagaagag atatgaaaaa tatgccaaag ctcaagcttg
    29641 gtcgacagat agttcattat tgatgccaac agcttcatct ggtggttctc caattgtaag
    29701 taacgtagta ccattctcaa aaccatactc acaagttggt attaaggggg aaccatatat
    29761 ctttaaagga atgaaattgc aaaaagatat tgttacaaca aaagaatata acgaggtttt
    29821 taaaaaatgg caaaaagaaa aattggaatc caatagcaaa taccaaaaag aactagaaaa
    29881 acacattaaa t
//
LOCUS       DAVLVZ010000021        29103 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000021 DAVLVZ010000000
VERSION     DAVLVZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.021"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(52..1389)
                     /locus_tag="VYN77_000912"
     CDS             complement(52..1389)
                     /locus_tag="VYN77_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1558390.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(1399..1665)
                     /locus_tag="VYN77_000913"
     CDS             complement(1399..1665)
                     /locus_tag="VYN77_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1558391.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAIVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(1930..2316)
                     /gene="rplQ"
                     /locus_tag="VYN77_000914"
     CDS             complement(1930..2316)
                     /gene="rplQ"
                     /locus_tag="VYN77_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1558392.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(2328..3263)
                     /locus_tag="VYN77_000915"
     CDS             complement(2328..3263)
                     /locus_tag="VYN77_000915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1558393.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(3306..3689)
                     /gene="rpsK"
                     /locus_tag="VYN77_000916"
     CDS             complement(3306..3689)
                     /gene="rpsK"
                     /locus_tag="VYN77_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1558394.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(3707..4072)
                     /gene="rpsM"
                     /locus_tag="VYN77_000917"
     CDS             complement(3707..4072)
                     /gene="rpsM"
                     /locus_tag="VYN77_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1558395.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(4090..4206)
                     /gene="rpmJ"
                     /locus_tag="VYN77_000918"
     CDS             complement(4090..4206)
                     /gene="rpmJ"
                     /locus_tag="VYN77_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1558396.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(4231..4449)
                     /gene="infA"
                     /locus_tag="VYN77_000919"
     CDS             complement(4231..4449)
                     /gene="infA"
                     /locus_tag="VYN77_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1558397.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(4566..5204)
                     /locus_tag="VYN77_000920"
     CDS             complement(4566..5204)
                     /locus_tag="VYN77_000920"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1558398.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(5355..6665)
                     /gene="secY"
                     /locus_tag="VYN77_000921"
     CDS             complement(5355..6665)
                     /gene="secY"
                     /locus_tag="VYN77_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1558399.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(6678..7118)
                     /gene="rplO"
                     /locus_tag="VYN77_000922"
     CDS             complement(6678..7118)
                     /gene="rplO"
                     /locus_tag="VYN77_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1558400.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(7263..7487)
                     /gene="rpmD"
                     /locus_tag="VYN77_000923"
     CDS             complement(7263..7487)
                     /gene="rpmD"
                     /locus_tag="VYN77_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1558401.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(7459..7953)
                     /gene="rpsE"
                     /locus_tag="VYN77_000924"
     CDS             complement(7459..7953)
                     /gene="rpsE"
                     /locus_tag="VYN77_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1558402.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(7971..8327)
                     /gene="rplR"
                     /locus_tag="VYN77_000925"
     CDS             complement(7971..8327)
                     /gene="rplR"
                     /locus_tag="VYN77_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1558403.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTAGRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(8411..8947)
                     /gene="rplF"
                     /locus_tag="VYN77_000926"
     CDS             complement(8411..8947)
                     /gene="rplF"
                     /locus_tag="VYN77_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1558404.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(9139..9537)
                     /gene="rpsH"
                     /locus_tag="VYN77_000927"
     CDS             complement(9139..9537)
                     /gene="rpsH"
                     /locus_tag="VYN77_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1558405.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(9822..10091)
                     /gene="rpsN"
                     /locus_tag="VYN77_000928"
     CDS             complement(9822..10091)
                     /gene="rpsN"
                     /locus_tag="VYN77_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1558406.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(10109..10651)
                     /gene="rplE"
                     /locus_tag="VYN77_000929"
     CDS             complement(10109..10651)
                     /gene="rplE"
                     /locus_tag="VYN77_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1558407.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGSKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(10675..10980)
                     /gene="rplX"
                     /locus_tag="VYN77_000930"
     CDS             complement(10675..10980)
                     /gene="rplX"
                     /locus_tag="VYN77_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1558408.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(11058..11426)
                     /gene="rplN"
                     /locus_tag="VYN77_000931"
     CDS             complement(11058..11426)
                     /gene="rplN"
                     /locus_tag="VYN77_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1558409.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(11452..11712)
                     /gene="rpsQ"
                     /locus_tag="VYN77_000932"
     CDS             complement(11452..11712)
                     /gene="rpsQ"
                     /locus_tag="VYN77_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1558410.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(11737..11943)
                     /locus_tag="VYN77_000933"
     CDS             complement(11737..11943)
                     /locus_tag="VYN77_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1558411.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(11953..12366)
                     /gene="rplP"
                     /locus_tag="VYN77_000934"
     CDS             complement(11953..12366)
                     /gene="rplP"
                     /locus_tag="VYN77_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1558412.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(12370..13023)
                     /gene="rpsC"
                     /locus_tag="VYN77_000935"
     CDS             complement(12370..13023)
                     /gene="rpsC"
                     /locus_tag="VYN77_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1558413.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(13036..13380)
                     /gene="rplV"
                     /locus_tag="VYN77_000936"
     CDS             complement(13036..13380)
                     /gene="rplV"
                     /locus_tag="VYN77_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1558414.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(13392..13673)
                     /gene="rpsS"
                     /locus_tag="VYN77_000937"
     CDS             complement(13392..13673)
                     /gene="rpsS"
                     /locus_tag="VYN77_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1558415.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(13777..14610)
                     /gene="rplB"
                     /locus_tag="VYN77_000938"
     CDS             complement(13777..14610)
                     /gene="rplB"
                     /locus_tag="VYN77_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1558416.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(14628..14924)
                     /locus_tag="VYN77_000939"
     CDS             complement(14628..14924)
                     /locus_tag="VYN77_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1558417.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(14924..15547)
                     /gene="rplD"
                     /locus_tag="VYN77_000940"
     CDS             complement(14924..15547)
                     /gene="rplD"
                     /locus_tag="VYN77_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1558418.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(15572..16198)
                     /gene="rplC"
                     /locus_tag="VYN77_000941"
     CDS             complement(15572..16198)
                     /gene="rplC"
                     /locus_tag="VYN77_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1558419.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(16415..16723)
                     /gene="rpsJ"
                     /locus_tag="VYN77_000942"
     CDS             complement(16415..16723)
                     /gene="rpsJ"
                     /locus_tag="VYN77_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1558420.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(16990..17607)
                     /locus_tag="VYN77_000943"
     CDS             complement(16990..17607)
                     /locus_tag="VYN77_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV1558421.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(17604..18194)
                     /gene="nrdG"
                     /locus_tag="VYN77_000944"
     CDS             complement(17604..18194)
                     /gene="nrdG"
                     /locus_tag="VYN77_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1558422.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(18187..18693)
                     /locus_tag="VYN77_000945"
     CDS             complement(18187..18693)
                     /locus_tag="VYN77_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1558423.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(18737..18877)
                     /locus_tag="VYN77_000946"
     CDS             complement(18737..18877)
                     /locus_tag="VYN77_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558424.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(18889..21096)
                     /gene="nrdD"
                     /locus_tag="VYN77_000947"
     CDS             complement(18889..21096)
                     /gene="nrdD"
                     /locus_tag="VYN77_000947"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1558425.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHTAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(21216..22754)
                     /locus_tag="VYN77_000948"
     CDS             complement(21216..22754)
                     /locus_tag="VYN77_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1558426.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(22791..22934)
                     /locus_tag="VYN77_000949"
     CDS             complement(22791..22934)
                     /locus_tag="VYN77_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558427.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(23012..24544)
                     /gene="cls"
                     /locus_tag="VYN77_000950"
     CDS             complement(23012..24544)
                     /gene="cls"
                     /locus_tag="VYN77_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1558428.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(24690..25232)
                     /locus_tag="VYN77_000951"
     CDS             complement(24690..25232)
                     /locus_tag="VYN77_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1558429.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            complement(25229..26479)
                     /locus_tag="VYN77_000952"
     CDS             complement(25229..26479)
                     /locus_tag="VYN77_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1558430.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(26891..27196)
                     /locus_tag="VYN77_000953"
     CDS             complement(26891..27196)
                     /locus_tag="VYN77_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1558431.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(27212..27631)
                     /gene="ruvX"
                     /locus_tag="VYN77_000954"
     CDS             complement(27212..27631)
                     /gene="ruvX"
                     /locus_tag="VYN77_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1558432.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(27635..27901)
                     /locus_tag="VYN77_000955"
     CDS             complement(27635..27901)
                     /locus_tag="VYN77_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1558433.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(27988..28557)
                     /locus_tag="VYN77_000956"
     CDS             complement(27988..28557)
                     /locus_tag="VYN77_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1558434.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGTATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLVGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(28623..29021)
                     /gene="spx"
                     /locus_tag="VYN77_000957"
     CDS             complement(28623..29021)
                     /gene="spx"
                     /locus_tag="VYN77_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1558435.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT         8143 a         6504 c         5392 g         9064 t
ORIGIN      
        1 tatagtatac acatctaatc ttaaatagaa cttaaaattt ctcctatttt cttaattttt
       61 aaaactatga attggtgctg ggatttgtcc accgcgagag atgaagtcga cagacgaagc
      121 cccattaacc ctcataacgg gtgcagttcc taatagacca ccaaactcaa tcatatcgcc
      181 ttcttttcct ttgggaatga tacgaacagc tgttgttttc atgttgataa caccgattgc
      241 tgcttcatcc gcaatcatag ccgcaatagt ttcagcaggc gtatcttctg ggatggcaat
      301 catatccaat ccaacagaac agatagccgt catagcttct agtttttcta aattaagaga
      361 gccattttgc actgcagcaa tcattccttc atcctcagaa acagggataa aggcaccaga
      421 taaaccaccg acctggttac aggccatcac tccacccttt ttaacttggt cgttcaagag
      481 ggccaaggca gccgtcgttc catgcgtgcc aactgtttct agccccattt cctcaaggac
      541 acgtgccaca gagtctccaa ccgcaggggt tggtgccaaa ctcaagtcca caataccaaa
      601 ctccacaccc agtctctcac tggccatttg accaaccaat tgaccgatac gagtgatttt
      661 aaaggcagtt ttcttaactg tttcggctac tacatcaaag ctctgtccac gaactttttc
      721 caaagcacgt ttcacaacac caggaccaga aactccgaca ttgatgataa catctgcttc
      781 cccaacacca tgaaaggcac ccgccataaa tggattgtcc tcaacagcat tagcgaatac
      841 aaccaacttg gccactccca tatctgaaag atttgctgtt tccttgataa ttcgtcccat
      901 atctgccaca gccgtcatat taataccaga cttggttgag ccgatattga ctgacgagca
      961 gaccttatcc gtctcagcca aagcgcgagg aatggaattg atgagaatct catctccctt
     1021 ttgataacct ttttgtacta aggcagaaaa accaccaata aagtccacac caatctcttt
     1081 cgcagcctta tcaagcgctt ttgccagaac cacgtagtcc gtcgcatctg tcgctgcccc
     1141 aatcagagaa ataggtgtca ccgatacacg cttattaacg ataggaattc ccaactcagc
     1201 cgcaatttca tcaccaacag ctactaaatt agccgccttt gtcgtaattt tttgatagat
     1261 tttctccgca gcacgattga tatctggatc gatacagtcc aaaagagaaa tccccatggt
     1321 aatggttctg atatcgaaat tttgctcctc aatcatggcg atggtttcag taacttgtct
     1381 aatatccatg ataacctcct agatattata catagcttcg aaaatcgctg cactctgaat
     1441 attgattttt acattcaaag tttgcccaaa agcttcaaat tcattacgaa gataggtaaa
     1501 atcttgcttt tcatcactag atacaatagc catcatcgta aaatattcat ccaagacagt
     1561 ttgagagata tcgtcaatat tcaatcctaa ttctgcaatt ttaccagaaa cacctgcaac
     1621 aattccagat ttatctttac caacaacagt tataatcgct ttcataagca aactccaatt
     1681 cttcttaaaa taagaaacct taacattaaa caggattctt ttcaaatagt atagcataat
     1741 tctaacgaaa atactcaaaa acgcctgctt acgaagttgt tcttaagaaa aaaacaattt
     1801 cgtaaacaag cgtctactat cccaacttat gatgagtgtt cccgctagga ccgaaatcct
     1861 agcggtagac cagagctaga ctaagagcac aagactccat catcataaca ctcaacaaaa
     1921 ttgatgattt tatactaatt cgatgatcgc cattggcgct gcatcaccac gacgtggttc
     1981 agttttaagg atacgagtgt atccaccgtt acgttcagca taacgaggtg cgatttctga
     2041 gaacaatttt tgaagtgctg tagtagaagt gtacttatca gttgcttcat catagttttc
     2101 agatgcgatt tcattacgta cgaaagcagc tgcttgacga cgtgcatgca aatcaccacg
     2161 tttacctaga gtaatcattt tttcaacagt tttacggatt tctttagcac gagcttcagt
     2221 tgtcacgatt gattcgttga tcaaaaggtc agttgtcaaa tcgcgaagca ttgctttacg
     2281 ttgtgagcta gtgcgtccta gtttacggta agccatgtat tcctccttta tttatctttt
     2341 aatccaagac ccaaatcaat gagtttgagt ttcacttctt ccaaactctt gcgtccaaga
     2401 tttcgtactt tcatcatctc tgcttcagat ttttctgtca aatcatgcac agtattgata
     2461 ccggcacgtt ttaaacagtt gtatgaacgc acagacaagt ccagttcctc aatcgtacga
     2521 tctaaaatac ggtcgtcaga ttcagtatca gcttctttca tcacttcagt tgacttagca
     2581 atctcagtaa gatttgtaaa caaatcaaga tgttctgtca aaatacgtgc tgaaagccct
     2641 aaagcatctt ctggaataat tgttccattt gtcaagattt caagggttaa tttgtcgaaa
     2701 ccatcattgc tacctacacg agcaggttcc acttgatagt tgacttttgt aactggtgta
     2761 taaatagaat ctacagcaag tgttccaact ggtgcattat cctttttatt ttcatcagca
     2821 ggtacatatc cacgaccact gttaacagtc atagtcgctt ttagagaaga accttcaccg
     2881 attgtaaaga gataatgatc tggatttaca atttcaatat cgctatctgt caaaatgtca
     2941 ccagctgtta cttcagcagg accttcaaca tccagttcga tgattttttc gtcttcaacg
     3001 tacgatttca ctgcaattcc tttaatgttc agaatgattt gcatcacgtc ttcacgaaca
     3061 cctggaactg tgtcaaactc atgtaacaca ccatcaatgt tgatagatgt cacagctgct
     3121 cctggtagag aagctagaag tacacgacga agagagttac caagagttgt accgtagcca
     3181 cgttcaagtg gttcgattac aaacttgcca taatctttat tttcatcaat ttttgttata
     3241 tttggttttt caaactcgat catttagtta ctccctctta aacgaaaagc agtgtaatgc
     3301 gatgattata cacggcgacg ttttggagga cgagcaccat tgtgtggcac tggagtcaca
     3361 tcacgaattg ctgttacttc aagaccagcg gcagcaagcg cacgaatagc tgactcacga
     3421 ccagaacctg gaccttttac agtaacttca actgatttaa gaccgtgttc ttgtgcagat
     3481 ttagcagcag cttcagaagc catttgagca gcgaatggtg tagatttacg agaacctttg
     3541 aaaccaagag caccagctga tgaccaagca attgcattac catgcacatc agtaatcata
     3601 acaatagtgt tattaaatgt agcgtgaata tgagcaatac cagattcgat attctttttc
     3661 acacgacgtt tacgtgttgg tttagccaag acttttacct cctatattat tttttcttac
     3721 cagcaatcgc aacagcttta cctttacgag tgcgagcgtt gtttttagtg ttttgtccac
     3781 ggacaggaag tccacgacgg tgacggatac cacggtatga accgatttcc atcaaacgtt
     3841 tgatgttcaa gtttacttca cgacgaaggt caccttcaac tttgattgca tccacttcac
     3901 gacggatagc atcttcttga tctgatgtaa gatcacgtac acgaacatct tctgagattc
     3961 cagcagcagc caaaattttc ttagatgttg caagtccgat accataaaca taagtcaatg
     4021 agattactac gcgtttgtca tttggaatat caactccagc aatacgagcc atgttttctc
     4081 ctttctatct tatccttgac gttgtttgtg ttttggattt gctgggcaaa ttaccataac
     4141 acgaccatta cgacgaataa ctttacagta ttcgcaaatt ggtttgaccg atggtcttac
     4201 tttcatttct tatccctcca agtttttcga ttatttaaag cggtaagtga tacgtccacg
     4261 tgtcaagtca tatggactca tttcgacagt aacacgatct cccgctaaaa tacgaatata
     4321 gtttttacga attttaccag aaactgttgc taaaatctga tgtccatttt caagttcaac
     4381 cgtaaacatt gcattcggca ttgtatcaac tactttgcct tcaacttcaa tcacatcgtc
     4441 ttttgccacg caaaagcacc tccataaatt tcgattcgat gcctctagac acagagacaa
     4501 caattataag tcagactatt tcagtataac atttgtagcg gatttttgca agtgtgaaaa
     4561 acgctttatt tcaaatttgt caatactttt tcaatatctg agaagacatc attgatatct
     4621 tgattacctt cgatgtcatg aaccaaacct ttggcacggt agtgagcaat gattggttct
     4681 ccttgagcaa tattaacatc caaacgacgt tttactgtct caggcttatc atcttcacgt
     4741 tggtagtaat cttcttcttt atagtcaact ggtgggttaa agaccttgtg gaaagtttct
     4801 ccagttacgc ggtggatgat acggccactc aaacgttcca agaggctgtc agggttcact
     4861 tcaatattga taataccttc tagttcaatg ccaagttcag ccaatgtttt gtccaaggca
     4921 tgagcttgtt caattgtacg tgggtaacca tccaataaga atcctgtttc tttaatatca
     4981 tcttgtgaaa ggcgttcttt tacgattcca tttgtaactt cgtcaggaac caattcaccc
     5041 ttgtcaatat atgacttagc aagaacaccc atttcagttt gatttgccat tgcagcgcgg
     5101 aacatatcac ctgttgagat atgtgcaaca tggaattgtt ctacgatttt tgctgcttga
     5161 gttcccttac ctgcaccagg taagcccata atcaaaagat tcatgatttg atctccttat
     5221 tttgttttta aatagaagca aaaagttttt tcaccccgat ttaaaaacaa aatagaagag
     5281 gggagatcca actcttacaa atgtcagagt ttaaacctcc actccctcag tatttactga
     5341 ttcagtaaat acttttattc tgttctgtcc atgaaaccaa catacttacg tttcaatagg
     5401 taaccttcca attgcttgat tccttcgata cctgtagaga taatgatcaa gagacttgtt
     5461 ccaccaaagg caacaacatc agaaagacca aatacatctt tagctgcaat cggtaaaatg
     5521 gaaatcacac caaggaagag ggaaccaaca gttgcaagac gacgaagaag tttagacata
     5581 tattcttctg tacctttacc aggacgaact ccatggatat aggcaccact cttttgtagg
     5641 ctctctgctg ctttttcagg attaatctgt acaaacgtat agaagaatgt aaagagaata
     5701 atcaacaaag catacatggc aataccagtt ggagaagtag ttgccaacat ctcttgtgct
     5761 acccttaccc aagcccaatc atgacctgtg gcactcaaaa actgaagaat agccgcaggc
     5821 gctgcagtaa tcgaactggc aaagataaca gggataactc cagcaggatt tacctttaac
     5881 ggaaggtaag agctagatgg agcaccttgt gcaaccttag tatattggat tggaattttg
     5941 tattctgctt gttgaacata agttgtaaag taaataatca acaatacagt aataatcaaa
     6001 atgattacga aaatgataga tgaagtgata cggctacttg ggacgttcac aaagtagtcc
     6061 acatagatgc cctgaatcat ctctggaatt gaggaaacaa tcccggcaaa gataatcatg
     6121 gaaacaccgt ttccgtatcc cttatctgta atttgctcac ccaaccaagt gacaatcata
     6181 ctaccagctg ttaagatgat accaatcgtc agaaaaactt gtggagttaa agcagtttta
     6241 atcaattgag ctccagccaa ggtattaaaa ccagctgtaa tcccgataga ttgcacaaaa
     6301 gcgagaacta gagcaatata acgagtagct tgattcaatt ttcttcgacc tacttcccct
     6361 tgtttacccc actctacaaa cttgggtaaa atatccattt gcaagagttg gacaacaata
     6421 gaagcggtga tatagggact aactcctagg gcaaaaatcg aaaagttttt tagggcattc
     6481 cccgacacca agctcaacat gtttaagaag gataatccac ttaaagcatt caagctattg
     6541 gcattcacac caggaactgt aatgctagtt ccgatacgaa agaccaaaac gataaaaatt
     6601 gtaaataaaa tttttgatcg aacctgcttg actttaagag cttctcttaa taatttaaaa
     6661 aacataggtc acctctctta gatgacttct actgaaccac ctttagcagt gatagcttct
     6721 tcagctgatt tagagaattt agctgctttc acagtcaatt tcttagtcaa ctcaccgtta
     6781 ccaagaattt taatacctga cttttcagct ttaacaattc ctgcttcgat aagaacaact
     6841 ggagttactt cagcaccatc ttcaaagacg ttcaattggt caaggttcac aattgcgtat
     6901 tctttagcgt tgatgttagt gaatccacgt tttggaagac gacggaacaa tggagtttgt
     6961 ccaccttcaa aaccaaggcg aactccgcca ccgctacgag ctttttgacc tttttgacca
     7021 cgaccagatg ttttaccgtt acctgatgaa gtaccacgac caacgcggtt acgtacttta
     7081 cgagaacctt ctgcaggttt caattcatga agtttcattt ttattttctc ctcttttgta
     7141 aaatgctagc gccgataagg gagaaaaggt tgtctccccc atcaactcgc ctatacgatg
     7201 tcatcataga tgactatatc tagttttagg ggatggtaca gtgcacatcc cctaaaactt
     7261 cattagttta cttcttcaac tgttactaag tgagatactg ctgtaatcat accacggata
     7321 gcagcgttat cttctttaat aacagagctg ttcaatttgc caagtccaag tgctacaaca
     7381 gttttacgtt gtgatggaat gcgtccgatt ggagacttag tcaaagtaat tttaatttga
     7441 gccattttat cccctttctt atgccaaatc agaaactgaa ataccacgaa gggcagcaat
     7501 ttcttcagcg cgtttcaatt gtttcaaacc ttcaacagtt gcacgaacaa tgttgattgg
     7561 agtgttagaa ccaagtgatt tagatgtaat atctgccaca cctgccaatt ccacaacggc
     7621 acgaactgca ccaccagcgg caactccaga accttctaca gcaggtttca acaatacttt
     7681 agctccaccg aattctgaaa gaacttcgtg tgggattgtt gttccaacca taggaacttc
     7741 gatcaagttt ttcttagcat catctactgc tttacggatt gcttctggaa cttcttgagc
     7801 tttaccagta ccaaatccta cgcgaccatt gtggtcacca acaacaacaa gagctgcgaa
     7861 acgaagacga cgtccacctt taacaacttt tgtaacacgg ttgacagcaa ctacgcgttc
     7921 ttctaattca actgcattgt ctttaaatgc cattttctag tgtcctccta ttagaatttc
     7981 aatccgtttt cacgagctgc atcagccaaa gctttcacac gtccgtgata tagatatcca
     8041 ccgcggtcga acaccacttc tgaaatacct ttagcgtttg cacgttctgc aacgagttta
     8101 ccgacagcaa cggcttgttc agttttagtt ccttttgaaa cttctttatc aagagttgaa
     8161 gcacttgcga gcgttacacc cgctacgtca tcaatcactt gagcgtagat gcctgtatta
     8221 gaacggaata cgttcaaacg tgggcgacca gcagttccag agagttttcc gcgaacgcga
     8281 cggtggcgtt tttggcggag tttgttttta tctggttttg aaatcacagt tttcacctct
     8341 ttagttttaa atcgtgtgct atgcacaaag ttggaaaata ggttggtggt tgatgatcaa
     8401 ccactcaaca ttatttacct gttttacctt ctttacgacg aacgaattca ccaacgtaac
     8461 ggataccttt acctttatat ggttctggtg aacgaaggct acgtacgtaa gcagctgttt
     8521 gaccaactac ttcttttgaa attccgctaa caacgattgt tgttgggttt ggaagttcaa
     8581 aagtaattcc ttctggagct tcaacttcgt ctggatgaga tttaccaaca gccaaaacaa
     8641 gtttagatcc ttgaagctgt gcacggtaac caaccccacg catttcaagt tctttcttga
     8701 atccttctga tacaccaaca accatgttgt tcaaaagggc acgagtagtt ccgtggatag
     8761 ttttcatttc ttttgaatcg tttggacggt gaagagttac ttcagtacct tccacacgga
     8821 tttcaatatc ttttgagaac tcacgagtaa gttctccttt aggtcctttt acagttacaa
     8881 cgttgtcatt gttagcgagt tcaacaccag caggcaacac gataacttta ttaccaatac
     8941 gtgacatgtt tattttctcc tgttaaattg tcaggccaaa acggccagtt ttcacggggt
     9001 tcagatactt atttagttca agagctgaac tgaacacgct ctaagaactg tgcaaaatag
     9061 ataagttggc ttgtttgtaa acaaacttct ccaacttcct aattttgctt tgttctttac
     9121 gagctttgta tcttgatttt accaaacgta agcgataacc tcaccaccaa cattcttttg
     9181 gcgtgcttct ttatcagtaa gcaaaccttc agaagttgaa aggatggcaa ttccaagtcc
     9241 gttaagaact tttggaaggt cttcacgttt tttgtagaca cgaagtcctg gtttagaaac
     9301 acgtttcaag ttagtgataa ctttttcacc atttggtccg tatttaagaa atacacggat
     9361 gacgccttgt ttgtcatctt cgatgatttc aacgtttttt acaaaacctt cgcgtttaag
     9421 gatttcagca atcccttttt tgatgtttga tgcaggtact tcaagtactt cgtgtttagc
     9481 ttggttagca ttacgaatac gagttaggaa gtctgcgatt gggtcagtca taaccatttt
     9541 gtttttctcc tcttactagt agtttgcaag ttgcacttgc tagttaatac atgatacaaa
     9601 gagcgtaaca gcacgagcaa aaatagacaa tttaatgcag aagcaatgct ccaaagcaaa
     9661 ttggtctttt ttgccaaagc tgtagctcgt gttcaaattg gcaagcccaa ttgaacccgg
     9721 gctaaactct gtgtgaaaaa gataaacttt cctagaaact ttagtttctt cgtcaagttt
     9781 cctattttta cttggagttt tgacgccctt gatatcttaa attaccaaga tgcttttgtt
     9841 acaccaggaa tttgaccttt atgcgcaagt tcgcgaaaag cgatacgact cagaccaaat
     9901 ttgcggtaaa ctgaatgtgg gcgccccgta actctacaac gattatgtaa acgagtcggt
     9961 gaggcgttgc gaggtaattt agataaacct tcgtagtccc ctgccgcctt taatgcagca
    10021 cgtttttcag cataacggtc aacaattttt tggcgtttag cctctctagc taccattgat
    10081 tttttagcca ttagatttac ctcctatatt attttgcaaa aggcattcca aggcctgtaa
    10141 gcaatgcacg tgactcttcg tcagtgttag cagttgttac gataacgatg tcaagaccac
    10201 gagttttgtc aacgtcatcg aagttgattt ctgggaagat taattgttct ttcacaccaa
    10261 gtgtgtagtt cccgcgtcca tcaaatgatt ttgttggaac accgtggaag tcacgtacac
    10321 gtggaagtga aactgatacc aatttatcca agaattcgta catacgttca ccacgaaggg
    10381 taacttttga accgatcgca acaccttcac gaagacggaa gccggcgatt gattttttag
    10441 ctttagtgat aagtggtttt tgacctgaga taagtgccaa ttcttcagca gctttttcaa
    10501 ggcttttagc gtttgataca gcttcaccaa cacccatgtt caaaacaatc ttatctactt
    10561 taggcacagc catcactgat gagtagttga attgttctgt caaagcagga actacttcat
    10621 taagatattt ttcttttaaa cgatttgcca ttatacttct cctttccttc gtgattaatc
    10681 aagcacttcg cctgattttt tgttgtagcg aactttttta ccgtctacaa atttgtaacc
    10741 aacacgacca gctacaccat ttttgtccaa aacttgaacg tttgatacgt ggatagctgc
    10801 ttctttctcg atgataccac cttgaggaag ctcgttagtt ggacgttggt gtttcttaac
    10861 aatgttaaca ccttcaacga taactttgtt tacttttgga agggcagtaa ggacaacagc
    10921 ttctgttccc ttatctttac cagcgattac gcgaactttg tcgccttttt ttacaaacat
    10981 taggtttctc cttgattttt cttacgccca taagggcacc ctagcttgaa gctaggggac
    11041 tagtttgttc ctaaaaatta aagtacttct ggagcaagtg acacgatctt catgaagcca
    11101 ccttcacgca attcacgtgc aactgggcca aagatacgtg ttccgcgagg ggttttgtct
    11161 tcacggatga taactgctgc gttttcgtca aatttgatgt atgaaccatc agcacgacga
    11221 gcacctgatt tagtacgaac gataactgct ttaacaacgt cacctttttt aaccgcacca
    11281 ccaggagtag cttgttttac agatgccacg ataacatcac cgatgtttgc aaatttacgt
    11341 cctgaaccac caagaacttt gatagtcaag atttcgcgag caccgctgtt gtctgcgact
    11401 ttcaaacgag tttctgtttg aatcatttca gttttctcct ttcaggtttg attagatgat
    11461 gaccgcttct tcaacaactt ctacaagacg gaaacgtttt gtagctgaaa gcgggcgagt
    11521 ttccatgata cgtacgatat cgccttcttt ggcaacattg ttttcatcat gagctttgta
    11581 ttttttagag tagttaatac gtttaccata gactgggtgg ttacgttttg tttcaactac
    11641 aactgtgatt gtcttgtcca ttttgtcaga tacaacacgt ccaacaagaa ctttacgatt
    11701 attgcgttcc attgaaattt ctccttccct agtctattat ttcgcttcag attgaactgt
    11761 tttgatacga gcgatttgtt ttttaacttc tttcaagcga gctgtttgtt ccaattgacc
    11821 agtagcagct tggaaacgaa gttcaaacaa ttcttttttc aattcgtttt cgcgcttcgc
    11881 gagttcttct tgagaaagac cacgaagttc tttaacaaat tcttttactt cattaagttt
    11941 catgccttct ccttattctg cttcacgttt tacgaattta catttaactg gcaatttgtg
    12001 gctagcaagt cgaagcgctt cacgtgcaat ctcttcagat acaccagcga tttcgaacat
    12061 cactttacca cgtttaactg gtgctaccca accttcaggt gcccctttac cagatcccat
    12121 acgcacaccg atagctttag cagtgtatga tttgtgtggg aagattttaa tccaaacttt
    12181 accaccacgt ttcatgtaac gagtcatggc gatacgagca gcttcgattt ggcggttagt
    12241 gatccagtgg ctagttgtag cttgaagacc gtattcaccg aatgctactt cttttccacc
    12301 ttttgcttca ccgcgcattt ttccacggaa ctcacgacgg tgtttaacac gtttaggtac
    12361 taacattggt tatttacctc ctttagtgtt tttacgagct ggaagaactt caccacggta
    12421 gatccatact ttaacaccaa gtttaccgta tgtagtatct gcttcttccc aagcgtaatc
    12481 gatatctgca cgaagtgtgt gaagcggaac agttccttca gagtatcctt cagcacgggc
    12541 gatatctgca ccgttcaaac gacctgatac ttgagttttg attcctttag ctccagcacg
    12601 cattgcacgt tggattgctt gtttttgtgc acgacggaaa gcaacacgtt gctccaattg
    12661 acgagcaatt ccttcaccta caaggtgagc atccaaatca ggttgtttga tttcgatgat
    12721 gttgatgtgt acttgttttc cagtcaattt gttaagtttt gcacggagtg catcaacgtt
    12781 agcaccacct ttaccgataa ccatacctgg tttagcagtg tgaagtgaaa cgttaacttt
    12841 gtttactgcg cgttcgattt caatagttga aactgctgcg tcagcaagtt ctttttgaac
    12901 gaatttacgg attgcaagat cttcatgaag gtaatccgcg tattcttttt cagcatacca
    12961 tttggcatcc caatcacgga tgatgccgac acgcatacca attggatgta ctttttgacc
    13021 cacgatttta cctccttatt tttctgcaac agctacagtg atgtgagctg tacgtttgtt
    13081 gattggtgaa gctgaacctt tcgcacgtgg acggaaacgt ttcatagttg gtccttcgtt
    13141 tgcgaatgct tcagatacta ccaaattagc tttatccaaa ccaaagttgt tttcagcgtt
    13201 agctacagct gagttcaaaa ctttcaagat gatttcagca gctttgtttg gagtgaatgt
    13261 caagattgcg attgcatcgg ctacgctttt accacggatg ttatcaagaa caagacgtga
    13321 tttacgaggt gaaacacgta ctgtacgagc cattgcttta gctgaagtaa tttctgccat
    13381 ttatgttctc cttattttct acgtgttttc ttgtcgtctg cagcgtgacc tttgtaagta
    13441 cgagttggtg caaattcacc aagtttgtgg cctaccatgt cttcttggat gtaaacaggt
    13501 acgtgtttac gtccgtcata aactgcaata gtgtaaccaa tgaaacttgg gaagatcgtt
    13561 gaacgacgtg accaagtttt aataactttt ttcttttcgt cgttagcttg agcttcaact
    13621 tttttcatca aatgctcatc gacgaaaggt ccttttttaa gactgcgtcc catttttata
    13681 ttttctcctt taaatgttgt accacagcgg cttgcactcc tatggagcgc taccgagctg
    13741 gcggatttac tagttgctta agcgactagt ttaatattat ttctcgttgc gacgacgaac
    13801 gataagtttg tcagatttcg ctttcttgtt acgagtttta agaccaagag caggtttgcc
    13861 ccatggagta gatggtgctt tacgaccaac tggtgcttta ccttcaccac caccgtgtgg
    13921 gtgatcgtta gggttcatta cagaaccacg aactgttggg cggatacctt tccaacggct
    13981 acgtcctgct ttaccaaggt ttacaagtcc atgttgttcg tttccgacaa caccaactgt
    14041 agcacggcaa gttccaagaa tcatacgaac ttcacctgat tgaagacgaa caagaacata
    14101 tttaccttca gaacccaata cttgagcaga tgcaccagca gcacgtacca attcaccacc
    14161 acgacctggt ttcaactcga tgttgtgaat caaagtacca actgggatgt tagcaagtgg
    14221 aagagcgttt ccgactttga tatctgcttc tggacctgaa acgatacgtt gacctacttc
    14281 aagacctttt ggagcgatga tgtatgcttt cacaccgtca gtgtagtgta caagagcgat
    14341 gtttgcagaa cggtttggat cgtactcgat tgttttaaca actgcttcaa cgttgtcttt
    14401 attacgtttg aagtcaacca aacggtaaaa acgtttgtgt ccaccacctt ggtgacgaac
    14461 tgtgatacga ccgttgttgt tacgaccagc cttgctcttc aatgcaacaa gcaatgattt
    14521 ttcaggagtg cttgttgtga tttcagcgaa atccaaagaa gtcatattac ggcgaccgtt
    14581 tgttgttggt ttataaacac gaattcccac gatatttcct ccttagatta ttcagcttca
    14641 gcagcaaaca actcgattgc tttagaatca gctgtaagtg tgatgatagc ttttttagtt
    14701 ttgttagtaa aaccagtgta acgtccaaca cgtttagctt ttggttttac gttgattgtg
    14761 ttaacattgg caactttaac accttcgaaa gcagcttcaa cagcttgctt gatcaaaagt
    14821 ttgtgtgcac gagtgtcaac ttcaaataca tattttcctg cttcaagttg agccattgag
    14881 ctttcagtga tgacaggttt tttgataaca tcatacaaat tcattatgca agaacctcct
    14941 cgattttaga gatagctgct tgtgtgacaa gaagtttgtc gctatttgcg atgtcaagaa
    15001 cacttgcagt tgtagcagtt gcaactttca cgtttggaag gttacgagct gaaagagctg
    15061 cgaattcatt tccttcttca aggataacaa gaactttaga atcgatgctc aatgctgcaa
    15121 gaacttttgc aaattcagca gtttttggag ctgtaaatga aagagcgtct acagctacga
    15181 atttgttttc agcaactttt tcagagtaaa ctgatttaag agctaggcga cgaacttttt
    15241 gtggaagttt gtagccgtat gaacgtggag ttggtccgaa gacaacacca ccaccacgcc
    15301 attgtggtga gcggatagaa ccttgacgag cacgtccagt tcctttttga cgccatggtt
    15361 tgcgtccacc acctgatact gcagagcggt ttttaacagc gtgtgttcct tgacgaaggc
    15421 ttgcgcgttg gctgatgatt acatcaaaca caactgattc atttggttcg ataccaaata
    15481 ctgcatcgct aagaacaact tggccagctt ctttaccagt ttggtcaaat aatgttacgt
    15541 ttgccattgt gactgatttc ccctttcttt attatttacc agctttaact gctgatttga
    15601 tagtgataag agatttctta gcacctggta cgttaccttt gataaggata acgttctttt
    15661 ctggaacaac ttgtacaact tcaaggtttt gaattgttac gcggtcgcca cccatacgtc
    15721 ctgcaaggtt tttacctttg aatacgcggt taggtgcaac aggccccata gaacctggac
    15781 gacggtggta acgagaaccg tgagccattg gtccacgtga ttgtccgtgg cgtttgataa
    15841 caccttggaa acctttacct ttagaagtac ccgttacgtc aacaacgtct ccagctgcga
    15901 atgtttcaac tgtaatttca gcaccaactt ccaagccttc aacgtttttg aattcacgaa
    15961 tgaagcgctt aggagccgtg ttagctttcg ctacatgtcc tttagcaggt ttgttgctca
    16021 atacttcgcg tttgtcatcg aaaccaactt ggatagcgtt gtatccgtct gtttcaacag
    16081 ttttaacttg aagaacaacg tttggagttg cttcaataac tgttacaggg atcaattcgc
    16141 cagcttcagt gaagatttga gtcattccca cttttttccc taagattcct tttgtcatga
    16201 gaaaatagtt ccttttctat attttttatt caaaaagttt ttaacgagcg ttttttatgc
    16261 tcaagtctaa gatacaaagg gcgtcaaact caacgtgaaa ataggaaact gacgcagtgt
    16321 ctagcagaca ctaggaagtt tatctttttc acaccgagtt tagcccgtgt tcaattagat
    16381 tgaaacacca gcctctgctc ttttatattt tagattaaag tttgatttct acgtttacac
    16441 cacttggaag atccaatttc atcaaggcat caactgtttt ttgagttggg ttaacgatat
    16501 cgatcaaacg tttgtgtgta cgcatttcaa attgttcgcg agagtctttg tatttgtgag
    16561 tcgcacgaat gattgtgtag aggctacgtt cagttggaag tgggattgga cccgcaactt
    16621 gtgcacctgt acgagtagct gattctacga tttttgcagc cgctgtgtca agcgtacggt
    16681 gttcgtaagc tttcaaacgg atacggattt ttttgtttgc catctttttc tccttttcgt
    16741 ctatttaaga taataggcta gctccacaag aaaaccgacg cggtgttgcg tggcaatgca
    16801 accgagcgtg tcgcaacctc ttgcatcaaa actaaggctg taatttacag caccataata
    16861 gaataacaca aagcccctgc gattgcaagg gatttgttgc tgttttttcg tttttttggg
    16921 atgaaactgt tttcctactt tttctttcta aaattaggga ttttcttcta tatttcttct
    16981 atacataagt taagtcctct tgacatcaaa tttatcttct gattggtcca ctaagatatc
    17041 cgccttagac tcagtttctt tatagtatcg cagatactgc tcccgtctca tctgatggct
    17101 agccaatata aaggatgcat cgcgatttct cacagtcgta tctctagcaa ggcgtcgctt
    17161 taattcggtc tcctcatccg tgtagaaaca gatggttttt tcaaagagtt ccttgggtag
    17221 aaagccaaca gacatccctt cgacaatcaa aattggtttg gctccagaca agacctcact
    17281 agccttccaa ggttcttcaa ttgtcaagac atccataccc gcctgcaagg caaggatatc
    17341 tctctgcaaa ctctccagtt catgcgccac tggcagactg gctgtcacct tttgattcgg
    17401 cgcgtccttg ggtactacca gatggcgtcc tgaggtgata taaggatctg tctctagcaa
    17461 atttactgta gtagaatcta aagcttggta caattcctgt gcaaaggttg atttacctga
    17521 agctccatga ccgtatattc ccagtgtcct gactttcccc gtctcgatct ctgagactag
    17581 ttggtctact aagtcttttt tcttcattct ctcttcacct gttcatagct ttcttttccg
    17641 tcattgagct tgtcccaaat cactacttgc ccacttttga gcgatttttg cacatcgata
    17701 attcgttggt tggacgaacc tcgaaactgg agcatgagat ttctcttagt tcgatcatat
    17761 cttccatcga caagaatgtc aatcaatgat aagagttcca gtttatctgg agtttccaac
    17821 atcatttctt cccaagtgta gcccgtccag gaccaaatgt ccttgtctgg caattccttt
    17881 cggatgcgtt taactagagg caagagaatg ccagtattga gaaaaggctc ccctcccagc
    17941 aaagtcaagc cttgaacata gggttgggca aggtctgcca taatctgctc ttctaattct
    18001 gctgtatagg gaatgccagc attaaaagac caagtcgcaa cattataaca tccctcgcag
    18061 tgaaacatac agcctgatac atagagagag ttgcgcacgc cttcgccgtc cacaaagtta
    18121 aaggccttgt agtcaatgat acgaccttga ctaagttcct cgcttttcca ttcttgtggt
    18181 tttggattat tcattcgcta cctctatcca ataacgctcg actccattgc gagcatcctc
    18241 aaatattcca ccatttgcta gaatgactgc tctgctagca ggattattca cgctacaggt
    18301 caccagagct ttcttgatgt tcttttcctt agcaacttgc aagccctgac ggagagtctc
    18361 ttttgcataa cccttgcctc tttcagatgg acgaatggag tagccaatgt ggccaccttc
    18421 ttctagtaga aagttactga ggcgcaaccg gagattaaga aatccaactg cttgaccttt
    18481 ctcagaaaaa gccactaact gaattgcagg aacccatcct tcaggcagat taatccccat
    18541 ttcctgttcc tgattgcttt ctaaccagtc ttcatacaca aagttctctg tatcccagaa
    18601 accgccgtcg tgaggcgact gaaatttttc aaactctgtc atcatatcta aaacagcttt
    18661 cttatccgct aatcttggtc tgcgtaattc catccttacc tcccactatg agaaaagcga
    18721 gagctggctc tcacttttat tttttcttgt tcttgtttaa aaattgttct ctgaagctaa
    18781 gcaagcgttc tttcttacca gctccacctt tagagtgttt ctcgtgataa cgggtcactt
    18841 gtgcgcgccc cttatcgtct agttgatatt ttcccatttc atttctttct aatttgttac
    18901 ttgatgccca gctattttaa tcgttgaacc attcatatgt ttgacacgcg cagcgatttc
    18961 cttgtgacgc ccgttaacca ttggtctcgc ttgaggattg ccaagatagc cacaagtccg
    19021 tttaacaacg tctactgttt taggatcgct attgccacag tttggacaag caaaccctct
    19081 ctcagttggt tcaaaatccc cttcaaagtc acacttgtag caacggtcaa tcggagtatt
    19141 ggtgcctaga tagcctacac ggtcataagc ataatcccag acagcttcca aggcctttgg
    19201 attttgctga aggactggat actcacaata atggatgaaa ccacctgacg cacctgcttc
    19261 tggatagact ttctcaaagt ccaatttttc aaacggtgtt ggatttttac gaacatcgta
    19321 gtggaaagag ttggtgtagt attccttgtc tgtgatatca ggaatagagc caaacttgtc
    19381 tatatctagt cggcagaaac ggtctgtcag actttcggat ggtgttgagt agatagagaa
    19441 atggtagcca tattggtctg accactcttc tacacggcgt ttcatatcgt gaatgatgtc
    19501 tagcgtgaat tccttagcat ctggattact ttcccagctg ttaccaaaga aaactgtcgc
    19561 tacttcatac aagccgatat agcccagcga aacggtcgcc cgacgattct taaagagctg
    19621 gtcaacactt tcttctttac ctagacgatg gccaaaagca ccgtactgat aaaggatagg
    19681 agcattcgct ggtgtcgcct ctttagtgcg ttcgacacgg taaacaagag catcttctgc
    19741 gatattcatt cgctcgttga agatttccca gaacttattc atatcacctt cagactcaag
    19801 agcaatacga ggcagattaa ccgtcacaac acccagattc atgcgacctg aattgacttc
    19861 tacaccattt tcatccttcc acccttgaag gaaagaacgg cagcccatag gcaccttgaa
    19921 agaacctgtc aaatcaacaa tcttatcata agacaagacg tctggataca tccgcttggt
    19981 tgcacactct agagccaact gcttgatgtc atagttggga gttccttcct ctaagttgag
    20041 gcctctttta agcgtaaaga taagtttagg aaagatagcc gtacggtgtt ctgaaccaag
    20101 acccttgatg cgaatgttta aaatagcttt ttgaatttct cgttcaaaac gactggttcc
    20161 cagaccaaaa cctaacgaag taaaaggtgt ttgtccattt gaagtgaaga gagtattgat
    20221 ttcatactca agagattgca tggcatcgta gatgtccttt tgcgctttct tccaagcgta
    20281 atcttcctgt ttttcaggca atacccactc ttctgcatct ttgagatgtt tttgataatt
    20341 cttctctgca taaggcgcca aaatttcatc gatacggtca gctgaacagc caccgtactg
    20401 gctagaagca acgttggcaa tgatttgaga aatctgtgct gtcgcagtct ggatagactt
    20461 gggactctct acctctgcat ttccaatctt aaaaccattt tccaacatac ccttaaaatc
    20521 aatcaaacag cagttggtca taggggtata gggactgtag tccaaatcgt gatagtggat
    20581 atcccccttt tggtgggcat tggctacgtg cttaggaagc atttgcagtc cgattgattt
    20641 cccaacaatc cctgctgtca aatcacgctg agtgttaaag acatcactgt ctttattagc
    20701 gttttcattg acaactgtct ggtctttgtt gagaagttta tgaatactaa agttgatatc
    20761 cgtcgctttt gagcgctcaa aatccctctg tgtccgataa gtaatatact cctcagccag
    20821 cgcatattct ttggcttcaa ggagttcatg ttctacgata ttttgaattt cgtaaatctt
    20881 aattccctgt ggaaagcgac tatgaatttc tgtaataatt cgctcagtca gatcattgag
    20941 gcgtttttca accaggggtg tcacatccat aaccttgtca gccgccgtgt ggagagcctt
    21001 gtcaatcttg tccacatcaa atacaacacg tctcccatct cgtttctcga cgaagagagt
    21061 tggcaaaatt gtaatttttt cttctagtgc aatcatacgc atgctctttt ctaaaatgtt
    21121 ctttctaaaa agtattatac cactctaaaa agaaaaatca atatcttgtg ttaaaaatcc
    21181 taaaattttt aaaaatacac aagatattga tttggttatt taattttata gactttaaac
    21241 tctgagtaat cagtctttac ttgttggtaa ttttctttta agagtgtttc cacttcagac
    21301 cagactacca ccttgtcatt taccacaatc accttaggtt gtttttcttt caagtcatta
    21361 agtaacttat tacgattttc ctcagttgct gtatagtgca acggggacaa aatcgccgat
    21421 ggcgacaagc gctcactctt acgataaaga gtcgcagtat catcccatgc atagatagca
    21481 tcttccgtat ttgtctcctc ttttaccaaa tcagcaagac gattacgttc ttgataaggt
    21541 actggataaa ggacaaactt catcaaaaaa ggaacagaca aaagatagac aaacccaaaa
    21601 actagcagat atagatttcc tttgaagaaa cgactccata ggctaactgg cgtttctctt
    21661 cggcttcttc tagcactacg ccccctctgt tgactcttaa tattggttac caataaaagt
    21721 aggaaaacag ggaagacgac catcaaataa gaagcgtgca gaggctcttg tgaaaagaaa
    21781 agtaaaatca aacctaaaat taccacaaga cttgcaggaa ccgaaatgac gtatagttta
    21841 aagggtttgg attgaaagag acctaaaaag atacaaacca caaatcccaa accaagggac
    21901 aacaagccat aaaaaaacat attctctaaa attttagaag taaaatcaaa gcgaatactg
    21961 tcaataggat aacgaatacc actaatcgca tccccaaaac ttcctgttgc agcactatag
    22021 taggcagttg gataaaagac aagtgaaaaa cctaaagcca ctgcaagaaa ctgataaaac
    22081 ccatgcacaa aacgtctatg cccaaggtta aagaccaaca agcctaaact cactacagca
    22141 ataaacagga gcgatgataa gggagcaaag aaaaatccgc ctgccaaagc tagcccaatc
    22201 cgtacaaatc ccttatcatg gcttggattg cttaggtaag ccgcaactaa actaaaggct
    22261 gcgaataaga aaggaagcgc taaaagagtc gcatagcctc caccaaaagc aagacctgta
    22321 actagcatgt aaaaaatagt caccagatgt ccagcttggt ctccttgctc tgtcaaggtg
    22381 tccgctgatc taaaaaggaa aaatcctcct gctaccaagg ctaaccactc aaagatggca
    22441 aagaaaaatc cgccctgact cacgtaagtc agcaaataat aaagcaaacc ttgacttcca
    22501 taatagtcgc tgtaaatctt ccctgtctga tgaagcgccc aacctgcata aaaatcctgc
    22561 acttcttgtg cactcattaa gtcgagtaat agcggtactc ctagagttat ccccgttaca
    22621 agcgtactcc atagtaaaat tttcaccaaa ggaagacgac ttgattcacg atgatgcgat
    22681 tcttgttcga tttggtattc tagaggttca cgattctcct tatgaacttc ttctactcta
    22741 ccatacacac tcatatcgtt tctcctgttc aatttatctg tcttagtata tcaaaaagtc
    22801 ctaggattgt cagcttgcga tggctgtttg agtgattttt tgcatagttt cacaaaggtt
    22861 tcaatttccc gaaagcggtg taaatgtgtc tgtttgaact caatcatatc attcacttct
    22921 tccttagtta gcatgaattc ccctcctctt tctttctatt cgtaaaagac aagaaaaata
    22981 ggtctggttt gtatcctaag ttctagattt cttataagat aggggcgaat agctgggcga
    23041 tttcttttac aagcttttga taccagctcg ttttgataga atgaggatag acttcctgag
    23101 aatctgcaaa tatatttcga aaatctcggg cgatttccct tatagaagga gttttataga
    23161 gtaagactgc attttcataa tggtgtacca agctccggta gtcgagattg attgtcccca
    23221 ccaccgcaaa atcttcgtct accaacatct gcttactatg aataaaacct ggactatact
    23281 cataaatccg aacaccagca gaaagaaggt cgggataagc tcctctcgtg actaactgaa
    23341 tgaacttctt atctggtatg taaggggtga taattcgaac atcgacccct ctcatagctg
    23401 catttttgat tgtctctgtt aaatcataat ctataatcaa ataaggtgtc gtaatataga
    23461 ccgattctgt tgcttgattg attaaactct gataaacttt tttccctacc tgcgctcgaa
    23521 aaattggctt gggtccactt ccgtatggaa tggttaaacc gtcactcggg atagaatgat
    23581 tttctaaatg atattgatca aaatcactaa tttctcctcg attaatgtac caagtggtca
    23641 aaaataagcg tgtcagagct tttactgcta gtccgtctaa gcgaattcca ctatccttcc
    23701 aataaccaaa tctctcgacg tggttaatgt actcatctgc cagattgacc ccaccagtat
    23761 aggctatctg accatcaaca atcaatattt ttctatgatc tctgttatta taagccactg
    23821 tcaaacgagg aataacttta ttgaatttat gggcctcgat gcccagctga cgaagtcgat
    23881 gtgcataatc tcctgttaaa gtagccatac agccgatatc atcatagagc atcttaacct
    23941 ctacaccctg agctaccttt tgctctagta tatctagtat gcgattccac atcaaacctt
    24001 cttctataat gtaatattcc aagaaaataa atttctcagc ctttttaaga tcttctacca
    24061 tctttttcca catagcttct ccgttaggaa aaaatgtaga ggcagtttga tcatagatgt
    24121 cagcattggt atccatactc aataaggact tgatgactcc ataagctgcc ttgtcacttt
    24181 cttttaattt ctcttttagt agctggctat ttgcttcttg gaaatgcata gagcctagct
    24241 tcttcagttg tttgatttct tttttggaca atcgcctttc accaaacatc agatagagca
    24301 agggaccaaa tactggcaca aaggctacta acaaccaggt taccttattc tcaggagtcg
    24361 tattacggtt gactatcgaa atgattgtga tgatactcag tagaatgagg acagtaatcc
    24421 ataaaatggg agccatgcgt cctaaataaa gaaagagacc aaaaacaaga caaagttcaa
    24481 gtagcataat cgaaagacta aagccatact tggacatcaa taattgaaat ttcctatatt
    24541 tcatatcccc tccttgatag tttgaatgct aaaaacaagt aattgatact agtataacgc
    24601 aggtattcgg tagaaggcaa gtatttatga ttattttcta ggtaaaagta aaaaggctga
    24661 aaaatccagc cctttctact tgcatagtct tatttagtta aaacgatttg gtccgatggg
    24721 tctctatcca tatcatctac gacgatttga ttaccgttta cagtgtaaat tttgacatca
    24781 tcgccaataa tcatgcgttg atttgctgaa tcaaatgtaa cctgtttgac tttctgatcc
    24841 ccgtcagatt cgagctcagt ccatgtacca gtcgttccag ttaccactaa agtgatacgg
    24901 tctccgtcat cctgaccagt ataagtccca tcaatatcgg tcggttgagc aacaggttgg
    24961 ttttgtactg gagccgcttg attttggtta cctcctgcat ttgcagtatt ggtattctgt
    25021 tgagcaggct gttggacctg ctgagcaaca ggctgttgaa cctgttgagc tctttgtgaa
    25081 caagctacta agagactaaa acttgctaga gaagcaagag aaagtgtgaa tctttttaat
    25141 ttcatgatga atttcctttc tgctaccaat ttagagaaat tttctctaac cagcaattcc
    25201 cctagtataa caagttcaaa aaagggggtc aatttatctg ctcacggtcc agcaggtagc
    25261 cccgtacttc tgagataaaa tagagagacc ctgtaacgaa cagcaaatcc tgagcatctg
    25321 ctctttcttc aaaatcgctg ataaattctc ggtaagaaga aactatatcg taacctgtca
    25381 catccctttc gtccaaagcc ccctgatagt caaagccggt caccttgagt tccacctgag
    25441 gcaatttttc agtcaggtaa cctaacatcc cttgataatc cttccgtttc aaggctccaa
    25501 aaaggatttg aggtcgatag ccttcctgct ctttttcttt gataaactca accaagcgag
    25561 tcaaggcagg gaggttatga gcaccatcca agtaaatctg tgggcgaata cgctccaagc
    25621 gaccagccca atgggtctgt tccaaggcct ttcttacagc ctgttcatca acagcttcct
    25681 ttccttctcc cataaaaaga agaaaagttt gcaacgccaa ggctgcattc tcctgctgat
    25741 agactccttc taagcctatg ttcagctgtg aaagatttag taaagagctt gaaaaatcac
    25801 cattcagcat taaaaaatct tgacctgcct gataaaggtt aacagctaaa gattcggctt
    25861 ttttctgaca gacaagccta gcttctggag gcaatttcgc aatcactgcc ttcttaccag
    25921 ccttgaaaat accagctttc tgctctgcaa ttgcttctag actatcaccc aaggtttctt
    25981 gatggtcaag cccaatggag gtgatgacag caagctctcc agttaccaca ttggtcgtgt
    26041 caagtaagcc accaattccc acttctaata aaaccaaatc cacctcttgc tccctaaaat
    26101 aaagaaaagc aactaaggtc agcaattcaa aaaaggacaa ctgggcagga gtttgcagaa
    26161 gcgttttctc catctccttg acctgatcag tcaaacggat aaagtctgcg tctgctatag
    26221 gttgcccatt aatgcagatt cggtcattga tagagacgat atgaggggag gtaaaggtcg
    26281 caactttttt gccatgcccc ataaataatt ccctcataaa agcaatggta gatcctttcc
    26341 cattagtccc tgttacgtgg ataatagggt aagactgctc aggatttcct aacaaatcca
    26401 ccgcttgctg cattcggccc aaacctgatc gaaaattcaa accaatccga ctatggagcc
    26461 attcttctac ttcaaacata cacatctcct tgacaaaagt ccaatcaatt atcgcattaa
    26521 agtatggtta ctaataaaaa caaggccagg attttcgtcc cgacctctta cctggttagc
    26581 taataactag ctactatgaa tgtgaacgtg ggctaaaaat atccactaga tgttccaact
    26641 cttctcaatt tctaggagtt ggggtaaaaa tgttcactgg acgttccaac tcttccccat
    26701 ttctgggagt tgggctgata cagtctccca gacttaccag ctctatctta tttaaaggag
    26761 ctgggctaaa aacgtccccc gaacctacca gttctctctt atttaaagga actgggctaa
    26821 aaacatccac tggacgttcc aactcttccc catttctggg agttgggctg atacagtctc
    26881 tcagactgta tcactcctcc ataaagctgt tgaagacttc ttcaatcatg ttccattcgt
    26941 cttctgagtc ttctgggatt ggttgcaatt cgccttctgt tccatcttcg ttttcgatga
    27001 atgagtaagc ttggatttca acttgtccgt cttcgtcttc ttctgcgtta actggtacta
    27061 gaagaacata gtttttacca aattcttctt ttccatcaat tgtcaaaagg atttcaaaca
    27121 aggtttcatt tccttgctca tctactagtg tgattagttc acgttcttcg tggtcgtggt
    27181 tatgatcgtg tgacatagcc tctcctttat attaaaattt tctatctaaa taattttgta
    27241 aaatcagctg agctgctaac ttatcaatga ctttcttgcg cttattgcga ctgatatctg
    27301 cttgttcaat caacatgcgc tcagcagcca ctgttgtcaa gcgttcatcc tgatagtcta
    27361 ctggtaaacc aaaaaactct tctagctttg ctccgtaggc ttgactagct tctacgcgcg
    27421 gtccacttgt attgttcatg tttttaggca agcccactac aaatcgttcc accttgtaag
    27481 tatcaaccaa ttccttaacg cggtcaaaac caaattggcc ttgttcttca tttatctgga
    27541 tgatttcaag cccttgagct gtaaaaccaa gcggatcgct aatcgccacc cctaccgttt
    27601 ttgaaccgac gtccaatccc ataattctca taggttatag atcgactcct tgtcctttga
    27661 ggtagtagcg aaccaattcc tcaacgattt catcacgctc atacttacgg atttgatttc
    27721 gtgcattatt ataacgagga acgtaggcag ggtctccact caatacgtaa cctacgattt
    27781 ggttaattgg gttgtaaccc ttatcgttca acgaagcata aacatctgtc aaagtttcgc
    27841 taatttcttt tttattggaa tcgtccaatt taaaacgtac tgtttcttca gtaaatccca
    27901 ttctaacacc ctctttcctt agaatagtac cattatagca taattcctta ccttctacaa
    27961 ttcaggcagt ctatttattt ggattttcta ctgttctgtc gcaccatttg ccaatctgtc
    28021 tgaaatatat ttgcttggtt catttttcaa aagattttcc aaaccaatat tcttcagatg
    28081 ttccaactgg gaagccttct tgacatccag aacttgaaaa tcaaaactag tcgttgtttg
    28141 aagttccgtt gcgctcaata gttttgtttc aagtttgaaa cctaccaatt tacgagcttc
    28201 aatgatagac ttatccttct cctccgcttc aagaagagct ttttgagttt cttccactcc
    28261 atgttggtca atataagtct tcaactcatc agaaactgga cgtttttgtt gaatcgttaa
    28321 gctcaaaaaa gtcttgtcct tgtatgtaat ggtttgagtt tgctggctac catcatctga
    28381 cttgggcaag accaaagtct tggttacaac tgtattcttc tcggcatttt caataactgg
    28441 caatgccgac tgaagcgtat ccttttctgt ttttgtagct gttccagttt cttttttctg
    28501 tccgcaacca accaggacaa aaaggaaagc tagactaaca agaactattt ttttcatttc
    28561 tctcttcttt ctttttgaaa ttaaaataga ataagactgg gaagtgctcc cagccttgat
    28621 gtttatagag ctgcacgtaa acgtgcttct gcattttcta cattacggac agagcgtggt
    28681 aggaaggcac gaatatcgtc ttccttgtag ccaacttgca ggcgtttttc atctacaagg
    28741 attgggctct ttaaaattct cggtgtttcc ataatcagat tgagaacttc attgacactc
    28801 aaatcttcaa tatccactcc aagggctttg gcatagcgat ttttagacga aacgatgctg
    28861 gctattccgt tatctgtttt ggttagaata tccagtaatt cttctctcgt aattccttct
    28921 ttaccaaggt tttgttcttt ataacttaac tggtgggcat tgagccaggt ttttgctttt
    28981 ttacagctag tacaacttga gactgtataa attttaatca tgtacctacc cctttcgcta
    29041 catgttacta tcagtttagt ctattatacc ataaaaaaca tccgacttgc gacctatttt
    29101 taa
//
LOCUS       DAVLVZ010000022        27903 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000022 DAVLVZ010000000
VERSION     DAVLVZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27903
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.022"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..1222)
                     /locus_tag="VYN77_000958"
     CDS             complement(<1..1222)
                     /locus_tag="VYN77_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HEV1558436.1"
                     /translation="MKITIKRYSLPEILGLAALAIFLLFSLLDVSFYVQYISPMLYKA
                     AIFLIIMLIILKEFVSGSNSFESLLGLLGVSTLYFIIGSVTSYTSFIVLGLSLIYSLR
                     DIPFSKVVKVAFIISVAMLFLVILSSKIGFIPDYIEFSSTRVRHYLGFRYSLFPSTIM
                     MNIIAISFFLKQETVSYQRLFILFLVSMYLYLETDSRLTFISSSLLLLANLAMKLIPT
                     VIEKFSLLLKFFTLTYFVNAYLSYWISKNYLRTSNALLNHFFHQADQFLGGRIYLSNR
                     SLSLYGYGILGQKIAWVGNALNAQGERSTDAYLYVDNLYIQILQHFGLIALIIILSLL
                     TATLVKLLRKGQIVLSIIIVSLSFHALIDDLILSIHYNIFWILLGSLIYSNYQFSEER
                     YGEVENDSLRRTVRE"
     gene            complement(1231..2037)
                     /locus_tag="VYN77_000959"
     CDS             complement(1231..2037)
                     /locus_tag="VYN77_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HEV1558437.1"
                     /translation="MERSRLIDVKIIVATHKEVKMPQDNSLYLPIHVGRDGKSDIGFI
                     GDNTGDNISSLNPYYCELTGLYWAWKNLDYNYLGLVHYRRYFTNKSQGYNENVNMDDV
                     ILSRSNVEILLEKSDVIVPKKRKYYIETLYSHYAHTLNGEHLDLARKIIEQNSSEYLS
                     SFDKVMKQRSGYMFNMFIMKKGLLDDYLPWLFSILDTMYEQMDLTDYTSFESRLFGRV
                     SELLFNVWLCKKGITPKEVPFMYMERVDLFEKGKSFLMAKFFGKKYGQSF"
     gene            complement(2024..3391)
                     /locus_tag="VYN77_000960"
     CDS             complement(2024..3391)
                     /locus_tag="VYN77_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1558438.1"
                     /translation="MNGKILRSSLAIIQSFLVILLTYLLSAVRETEIVSTTAIALYIL
                     HYFVFYISDYGQDFFKRRYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LTLHALLVYVLNLFIKWYWKRAYLNFKGSKKILLLTATSHVEKVLDRLIESNEVVGKL
                     VAVSVLDKPDFQHDCLKVVAEGEIVNFATHEVVDEVFINLPSEKYNFGELVSQFETMG
                     IDVTVNLNAFDRSLARNKQIREMAGLNVVTFSTTFYKTSHIMAKRIIDIMGALVGLIL
                     CGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPE
                     QKRRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDDWTIWKDIEILLKTVKVVLM
                     KDGAK"
     gene            complement(3406..4095)
                     /locus_tag="VYN77_000961"
     CDS             complement(3406..4095)
                     /locus_tag="VYN77_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV1558439.1"
                     /translation="MPTLEISQAKLDSVKKAEEYYNALCTNLQLSGDGLKVFSITSVK
                     IGEGKSTTSANIAWAFARAGYKTLLIDGDIRNSVMLGVFKARNKITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFTTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITRNCDASILVTEAGEINRRDIQKAKEQLEHTGKPFLGIVLNKFDTSVDKYGSY
                     GNYGDYGKNKK"
     gene            complement(4105..4797)
                     /locus_tag="VYN77_000962"
     CDS             complement(4105..4797)
                     /locus_tag="VYN77_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEV1558440.1"
                     /translation="MKEQNTIEIDVFQLFKTLWKRKLMILIVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGSYLVKDYREIILSQDALEKVATNLKLD
                     MPAKTLASKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIVAVTRVSDVT
                     TLEEARPATTPSSPNVRRNSLFGFLGGAVVTVIAVLLIELLDTRVKRPEDVEDVLKIP
                     LLGLVPDFDKIK"
     gene            complement(4806..5537)
                     /gene="cps4B"
                     /locus_tag="VYN77_000963"
     CDS             complement(4806..5537)
                     /gene="cps4B"
                     /locus_tag="VYN77_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEV1558441.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLTESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLEKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDRGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQDLVHIIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            complement(5539..6984)
                     /gene="cps4A"
                     /locus_tag="VYN77_000964"
     CDS             complement(5539..6984)
                     /gene="cps4A"
                     /locus_tag="VYN77_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV1558442.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVGFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLVIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSDIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGIDVYNDQ
                     EFTAHTNGKYYPAGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRTDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            7209..7731
                     /locus_tag="VYN77_000965"
                     /pseudo
     CDS             7209..7731
                     /locus_tag="VYN77_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7724..7897)
                     /locus_tag="VYN77_000966"
     CDS             complement(7724..7897)
                     /locus_tag="VYN77_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV1558443.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNTVN"
     gene            complement(8057..9664)
                     /locus_tag="VYN77_000967"
     CDS             complement(8057..9664)
                     /locus_tag="VYN77_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1558444.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNEDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGAKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMISVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKQVLAPWDAFCVELL"
     gene            9817..11301
                     /locus_tag="VYN77_000968"
     CDS             9817..11301
                     /locus_tag="VYN77_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1558445.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQLAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGESPYA
                     SPTDMGVNMVGFAITDDEAAIEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVTARQKAEETGGPALALELPTGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            11396..11878
                     /locus_tag="VYN77_000969"
     CDS             11396..11878
                     /locus_tag="VYN77_000969"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1558446.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDVISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAEIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(12048..14153)
                     /locus_tag="VYN77_000970"
     CDS             complement(12048..14153)
                     /locus_tag="VYN77_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1558447.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNVEVDGKMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     EAEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGPTGVGKTELAKQLALDMFGTKDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKGIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGGLVIREKA"
     gene            complement(14697..15677)
                     /locus_tag="VYN77_000971"
     CDS             complement(14697..15677)
                     /locus_tag="VYN77_000971"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1558448.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLIASVLVAFFFALFSNQLSNNVGMILFILVLYGLIGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGIDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFVFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKMTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(15679..17931)
                     /locus_tag="VYN77_000972"
     CDS             complement(15679..17931)
                     /locus_tag="VYN77_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEV1558449.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     LESSLNTILAGTDGIITYEKDRVGNIVPGTELVSQQTVDGKDVYTTLSSPLQSFMETQ
                     MDAFLEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITEDFVWRDILYQSNYE
                     PGSAMKVMTLASSIDNNTFPSGEYFNSSEFKIADATTRDWDVNEGLTTGGMMTFLQGF
                     AHSSNVGMSLLEQKMGDATWLDYLKRFKFGVPTRFGLTDEYAGQLPADNIVSIAQSSF
                     GQGISVTQTQMLRAFTAIANDGVMLEPKFISAIYDTNNQSVRKSQKEIVGNPVSKEAA
                     STTRNHMILVGTDPLYGTMYNHYTGKPIITVPGQNVAVKSGTAQIADEKNGGYLVGST
                     NYIFSVVTMNPAENPDFILYVTVQQPEHYSGIQLGEFATPILERASAMKESLNLQSPA
                     KNLDKVTTESSYAMPSIKDISPGELAEALRRNIVQPIVVGTGTKIKETSVEEGTNLAP
                     NQQVLLLSDKVEEIPDMYGWKKETAETFAKWLDIELEFEGSGSVVQKQDVRTNTAIKN
                     IKKITLTLGD"
     gene            complement(17935..18252)
                     /gene="ftsL"
                     /locus_tag="VYN77_000973"
     CDS             complement(17935..18252)
                     /gene="ftsL"
                     /locus_tag="VYN77_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1558450.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(18264..19214)
                     /gene="rsmH"
                     /locus_tag="VYN77_000974"
     CDS             complement(18264..19214)
                     /gene="rsmH"
                     /locus_tag="VYN77_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1558451.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            19380..19574
                     /locus_tag="VYN77_000975"
     CDS             19380..19574
                     /locus_tag="VYN77_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1558452.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            19590..20132
                     /locus_tag="VYN77_000976"
     CDS             19590..20132
                     /locus_tag="VYN77_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEV1558453.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITAIDPDMLSEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLRILIGEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            20143..20385
                     /locus_tag="VYN77_000977"
     CDS             20143..20385
                     /locus_tag="VYN77_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558454.1"
                     /translation="MKKEKRQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            20396..20731
                     /locus_tag="VYN77_000978"
     CDS             20396..20731
                     /locus_tag="VYN77_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558455.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(20841..21842)
                     /locus_tag="VYN77_000979"
     CDS             complement(20841..21842)
                     /locus_tag="VYN77_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1558456.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(21902..23803)
                     /locus_tag="VYN77_000980"
     CDS             complement(21902..23803)
                     /locus_tag="VYN77_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1558457.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRILDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSILFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWFLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQKLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(23825..24118)
                     /locus_tag="VYN77_000981"
     CDS             complement(23825..24118)
                     /locus_tag="VYN77_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1558458.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(24118..24936)
                     /locus_tag="VYN77_000982"
     CDS             complement(24118..24936)
                     /locus_tag="VYN77_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1558459.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(24923..25702)
                     /locus_tag="VYN77_000983"
     CDS             complement(24923..25702)
                     /locus_tag="VYN77_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1558460.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(25717..26208)
                     /locus_tag="VYN77_000984"
     CDS             complement(25717..26208)
                     /locus_tag="VYN77_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1558461.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(26219..27409)
                     /locus_tag="VYN77_000985"
     CDS             complement(26219..27409)
                     /locus_tag="VYN77_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEV1558462.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(27421..27855)
                     /locus_tag="VYN77_000986"
     CDS             complement(27421..27855)
                     /locus_tag="VYN77_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1558463.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMPADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHIFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
BASE COUNT         8360 a         6156 c         4870 g         8517 t
ORIGIN      
        1 cttcccttac agttctacgt aaggaatcat tctcaacttc tccatacctt tcttcagaaa
       61 actgataatt tgagtagatt aaactaccta ataatatcca aaaaatatta taatggatac
      121 tcaatatcaa atcgtcaatc aaagcatgga aactcagaga aactataata atacttaaga
      181 caatttgtcc ctttctcaaa agcttcacta aagtcgcagt cagtaaactt aaaataataa
      241 ttaaagctat cagcccaaaa tgttgaagaa tctgaatata tagattatct acatataaat
      301 atgcatccgt acttcgttct ccttgcgcat tcaaggcgtt tccaacccaa gcaattttct
      361 gcccaagaat cccataacca tataaactta acgaacgatt agataaataa atacgccccc
      421 ctaagaattg atctgcttga tgaaaaaaat gattcaacaa ggcatttgat gtccttaagt
      481 aattttttga aatccaataa cttaaatagg cattaacaaa ataggtaagc gtgaaaaatt
      541 ttaataaaag actaaatttt tcaataacag tagggattag cttcatagcc aaattagcta
      601 atagtaacaa actcgaactg ataaaagtca gtcgtgaatc agtttctaaa tataggtaca
      661 ttgaaactaa aaataatata aaaaggcgct gatacgatac tgtttcttgc ttcaaaaaaa
      721 atgatatagc tataatattc atcatgatcg ttgatggaaa taatgaatat cggaatccaa
      781 gatagtgtcg aactctagta gatgaaaact caatgtaatc aggaataaaa cctatcttac
      841 tactcaatat cacaaggaac aacatagcaa cactaatgat aaaggctact ttgactactt
      901 ttgaaaaggg aatatccctc aagctatata tcagagatag ccctaagact ataaaagaag
      961 tatagctcgt tacactacct atgataaaat agagcgtaga aacccctagt aaacccaata
     1021 gagactcaaa gctattactc ccagatacaa attccttcaa aattattagc ataattatca
     1081 aaaagattgc agctttatat agcataggag atatatactg aacgtaaaaa cttacatcca
     1141 atagcgaaaa taggaggaat atagctaatg ctgctagtcc taaaatttct ggcagtgaat
     1201 accttttgat agtgattttc atattccctc ctagaaactc tgtccatact tctttccaaa
     1261 aaatttagcc attaaaaaag attttccttt ttcaaacaaa tccactctct ccatgtacat
     1321 aaatggtacc tctttaggcg ttattccttt tttacataac caaacattaa acaacaactc
     1381 actaactcgc ccgaataaac gtgactcaaa tgaagtatag tcggtcaagt ccatctgttc
     1441 gtacatagta tccagaatag aaaaaagcca cggtaaataa tcatctaata gtcctttttt
     1501 catgataaac atattgaaca tataaccgct tctttgtttc atcactttat caaaggatga
     1561 aagatactct gaactatttt gctcaataat tttcctagca agatccagat gttctccgtt
     1621 aagggtatgg gcataatgtg aataaagagt ttcaatataa tactttcgct tctttggaac
     1681 tattacgtca gatttctcta ataatatttc aacattagat cgagacaaaa tgacgtcatc
     1741 catattgaca ttttcattat acccttgaga tttatttgta aaataacgac ggtaatgaac
     1801 tagacctaag taattataat caagattctt ccatgcccaa taaagtcccg tcaactcaca
     1861 ataatatgga tttagagagg atatattatc gccagtatta tcaccgataa aaccaatatc
     1921 tgatttaccg tctctcccaa catgtattgg aaggtaaaga ctattgtctt gaggcatttt
     1981 aacctcttta tgtgttgcca caatgatttt tacatctatc aatctacttc gctccatcct
     2041 tcatcaatac aactttaact gtcttcaata aaatctcaat atctttccaa attgtccaat
     2101 cgtctatata ggctacatct aatttgacaa cttcatcaaa attcttaatc tcacttcgtc
     2161 cactgacctg ccataagcct gttataccag gtttaaaact tagccgacgt ttttgttctg
     2221 gggtatagtg ttcatactcg tccactgttg gtggccgtgt gccaaccaaa ctcatatctc
     2281 cctttagaac attataaaac tgtggtagct cgtccaagct agtcttccgt ataaaacgac
     2341 caattttcgt gatacgagga tcatcgtcca ccttaaacat tccaccctgc atggtatttt
     2401 gttccatgag ttctcttttt ttcgcctcgg catctacaca catagagcga aacttataaa
     2461 aagtgaactg acgaccattt tttcctatac gcgtctgagc aaaaatagca gaacccccat
     2521 cctttcgaat caaaggaacc agtacaatac tgactaaacc acatagtatc agccctacca
     2581 atgcacccat gatatcaata atccgcttag ccattatatg actagtctta taaaatgttg
     2641 tagaaaaagt cacaacgttt aatcctgcca tctcacgaat ttgcttgtta cgtgccaaac
     2701 tacgatcaaa agcatttaga ttaactgtta catcaattcc catcgtttca aactgagaga
     2761 caagctctcc aaaattgtat ttttcacttg gaagattgat aaagacttca tcgaccacct
     2821 catgagtcgc aaagtttact atctccccct ctgctactac ctttaaacaa tcatgctgaa
     2881 aatctggttt atctaagaca ctgacggcta ccaacttccc aacaacctca tttgattcta
     2941 ttaatctatc cagtaccttt tcgacatgag aagttgctgt aagtaggaga atcttcttac
     3001 ttcctttaaa gttgagataa gcccgcttcc aataccactt gataaatagg tttagcacat
     3061 agactaagag agcatgtaat gtgaggaagt aaatcatgcc tcgtctggaa atactaaatc
     3121 gatcctctaa gaaaaaatta gaaatactaa tcgctagtgc aaagaatagg atatatttca
     3181 atgtctggac aagttcaatc aaatatctcc ttttaaagaa atcctgtcca taatcactga
     3241 tataaaagac aaaataatgg aggatataaa gtgcaatagc tgttgttgaa acaatctccg
     3301 tttctctcac agcactaagt agataagtca ataaaataac aagaaaactc tggattatgg
     3361 ccaatgaaga ccttaatatt tttccattca tctctatccc ccgacctatt ttttattttt
     3421 cccgtaatct ccataatttc cataagaacc gtatttgtct actgaagtat cgaatttatt
     3481 caacacaatt cccaaaaacg gctttcctgt gtgttccaac tgttcttttg ctttttgaat
     3541 atcccgacga tttatttcac ctgcctccgt cactaaaata gaagcatcgc agtttcgcgt
     3601 aataatagcc gcatcaatca cgacaccaac aggagcagta tctacaatga tgtagtcaaa
     3661 atatttacgc aaggtttcaa gcattgtagt gaaattctta ctttgaagaa gagctgtcgg
     3721 attcggtgac acagagccag cctgaattac aaagagattt tcgatattgg tatcacaaag
     3781 cccttgtgat aagtctgtag ttcctgataa aaattctgtc aggcctgtaa tcttattcct
     3841 tgctttaaag acacctaaca taacagaatt gcgaatatct ccatcaatca gcagcgtttt
     3901 gtaacctgca cgcgcaaaag cccaagcgat attggcggaa gtcgttgatt ttccttctcc
     3961 tattttcaca gaagtgatag aaaatacttt caaaccatct ccacttaact gtaggttcgt
     4021 gcacaaagcg ttataatatt cctctgcctt ttttacagaa tccaattttg cctgtgagat
     4081 ttctaatgtt ggcataactt cctcctattt tattttgtca aaatctggaa cgagccctaa
     4141 aagtggaatt ttcagtacat cttcaacatc ttcaggacgt ttcacacggg tgtcgagcaa
     4201 ctcaatcaaa agaacagcaa ttactgttac gactgctcct ccaagaaaac caaacaagga
     4261 attgcgtcga acatttggag aagagggagt cgtagctggt cgcgcttctt caagtgtcgt
     4321 tacatcagat actcgcgtta cagcgacgat cttttctgca gcaacttctc gtagagaatt
     4381 agcgatacga ctggcttcct ctggctgttt atccttgaca gagattgaga cgatacgagt
     4441 gtcagctggt acagtcactt gaactttgct ggctaacgtt tttgctggca tatccaactt
     4501 caaatttgtc gctacttttt ccaatgcatc ctgcgaaagg ataatctcac ggtagtcttt
     4561 taccagataa gatcctgcct gcaaatcctg atttgtcagc cccggcttgt ctccttgatt
     4621 gcgattcact acgtaaattc gcgtggtact cgtatattct ggcttaacaa taaaagtgct
     4681 atatgcaaaa gcccccgcac ctgtcacaag tgccactatt aaaatcatta gcttgcgttt
     4741 ccacaaggtt ttaaataatt gaaatacatc gatttctatc gtgttttgtt ctttcatcat
     4801 ttctcctaaa ttagttgatc cattacaatt tttcgaggat tgtctataaa aagttcctga
     4861 gccttcgctt ctccgtattt ttgggtaaca aggtcatatg cttctgccat atgaggaggt
     4921 ctaccgtcta gattgtgcat atcacttgca atgatatgaa ccaaatcctg ctctaaaaaa
     4981 tactgagctc tttttttcat gaatttataa cgttcgccaa aaagtttggg tttgaggaca
     5041 tgtgaactat ttacttgcgt gtaacagccc ctatcaatca gttctcgaac gcgtttttca
     5101 ttattttcaa gagcatcata gcgctcaatg tgggcaatga ctggagtaat tcccaacatc
     5161 aagatcttgc tcaaggcgct atgaatatcg cgataaggag tgttcatact gaactctatc
     5221 aaggcataac gactatcatt gagggtcgga atccgctttt tttccagctt atccagaaca
     5281 tctggtgtgt agtaaatttc agccccgtaa gcaatgacca agtcactcgc cacttcctta
     5341 gctatttccc gaacctgaag aaagttttct gctatcttct cttccggagt ttcaaacatg
     5401 cccttgcgac ggtgagaggt agagacaatg gttctcaccc cctgcctgta ggattctgtc
     5461 aagagagcct tgctttcctc tcttgacttg ggaccgtcat ctacatcaaa aacgatatgc
     5521 gaatggatgt ctatcatttc atctaccctc catcacatcc tgtatagctg ctttaactac
     5581 agctaaacta ctatcatcta tttccatcac atagaggtta ctgtctggca ttgcataaga
     5641 aggaagatcc gtccgacctg taccttttaa atcttgagaa tttactttat aattccctcc
     5701 actttctaac tgagcattga ccaaatttat catagtctca agtggcatat ttgtttggat
     5761 agaatcttgc aagctattaa tgatcgtgct ataatttttc agtgcttcgg ttgacgttaa
     5821 tttttgaagg atagccacaa tcaccttttg ttgattgcgc ccgcggtcac gatcgccatc
     5881 tgctagtgag tagcgctcac gaacaaaacc gagagcctgt tctgaatcaa gatgaacatt
     5941 gcctgcaggg taatactttc cattcgtatg ggcagtaaat tcttgatcat tataaacatc
     6001 aattccaccc aacaaatcaa tcaatttcaa aaacgaagtg aagttcaatc gcacatagta
     6061 attgatatcc actccataga gattttctaa ggtgtgaatg gacgaatcaa ctccataaat
     6121 gcccgcatgg gttaatttat ctttttgatt atttccacca tctgcgattg gtacataggc
     6181 atcacgtggc gttgtggtca agaggatttt cttggtatct cgattgacag tcatcaggat
     6241 attgacatct gatcgcgaca ccgaactaat agggccatag gtgtcaattc cactaacata
     6301 gatattgaaa gactgattct tagacgtctt aggagcttct acttttttag tgaatccctt
     6361 ggtataaatc ttttttatct tcgatgcgta gtctggatac tctgactcga tgatattttc
     6421 aaagacacta tttaggacaa tggccttagt ctcccctgca atcaaactct tgtaagctgc
     6481 caagtaagac gaactctggt tgaccgtcaa atcggtattc tgacttgact tgatatcagc
     6541 tagtagtttt tgaatatttt cattatcagt cccagtcggt gctgtcacac tcgtcagttg
     6601 cgtaacattt tcgatatcac tatctgctaa aacagcgaca ctgattgaat attctgagta
     6661 attagaagtc gcatttaaac gattggtcag tccaacaaac tgctgtactg caaagagcga
     6721 cacagagctg acaaggatag agaacagcaa cagaaaaata gtaaattttt cagctttttt
     6781 atagataacc aagagtagcc ctaccaaggc aactagtagg actaacgcag ttaccactag
     6841 gttaagatat ctaaaagcaa ggatattgta cttaaagatt aagaacaata aaaaaccaac
     6901 taacaataaa taaatagtca gcaaaacgat attaacactt cgcttcactt tctgtgaacc
     6961 tgatttttta aaacgtctac tcatgattaa cacctataca ttgaacatca tacgattata
     7021 tcactttttt acggtaatgt ctacaccttt attttcacta tctgcatctt taagtatctt
     7081 agtagacttc ccgcgaacca aaaatatagt aaaatgaaat aagaacatga taaaacgatc
     7141 aggacagtca aatcgatttc taacaacgtt ttagaagcag aggtgtacta ttctagtttc
     7201 aatctaatta tgaacaagac ccagaaaaag tagccttatt tcttaagaat tttaatagtt
     7261 taaagcacct agcacctgtt tagattgacg aaacaagatt cgatacttat ttttatcgag
     7321 aatatggtcg ctcattaaaa ggtcagttaa gaagaggcaa agtatctgga agaagatatc
     7381 agaggatttc tttggttgca ggtctaacaa atggtgagtt aattgctcca atgacttacg
     7441 aagagacgat gacgagcgac ttttttgaag cttggtttca gaaatttctc ttaccaacat
     7501 taaccacact atcggttatt attatggata atgtaagatt ccatagactg gggaagctag
     7561 aacttttatg cgaagagttt gggcataaac ttttaccttc ttcctcccta ctcacctgag
     7621 tacaatccta ttgagaaaac atgggctcat atcaaaaagc acctcaaaaa ggtattacca
     7681 agttgcaata ctttttacga ggctcttttg tcttgttctt gtttcaattg actgtattat
     7741 cagcgtaata gtctaataga tagttaaatg tagttggatt tccactaaag gttgatgagt
     7801 aagtttttgt atctgaacct gattggccgc aagcagctaa aagcaaagca gatgcaaaag
     7861 tcagacctgc actaaggaca cgcttcttta tgtttatctt ctttctcctt aatagtggga
     7921 atttgtaaag ttaattgaat ttcgagaatg aaagtttata aaccttggtt ataaaaaaac
     7981 aaaggatttc tgtcttttat acagtcctcc cttgttttta tatgatttca attttaaatt
     8041 tttctgcaaa aaatatttat agtaattcca cacagaaagc atcccatgga gccaagacct
     8101 gtttttcaag tacttcttta gccgcagtgt tttcaatcaa gacagatttg acttttcctt
     8161 ctactgtcaa gtcttgctct tcattggaca agttagccac aactaggaag cgacggtcgc
     8221 catccttacg tatataagca aagaccttat cagccgtatc aagcaattca aagtcagctc
     8281 gaactagcca gctattctcc ttgcgaattt ggaccagttt ctgataggta tagaaaatag
     8341 aatctggatt tgccagcgct tcttggacac tgatcatctc gtaatttgga ttaaccgcca
     8401 accaaggttg acctgttgag aaaccagcgt ttttgctctc gtcccattgc ataggggtac
     8461 gggcattgtc acgtccaata acacggatac tgtccatgat ttcttcaatc ggaacacctt
     8521 tttcaagagc ctcacgcgca tagttgagag attcaatatc ttctacttga tccagtgttt
     8581 caaacggata gttggtcatc ccaatctcct caccttggta gatataagga gttcctctca
     8641 tgagatgaag taagattgca aaggctttgg cagatttttc gcggtattct tggtcatttc
     8701 cccagattga gacaatacga gggaggtcat ggttgttcca gaagagggaa ttccagccgt
     8761 cctcaactcc taactctgtc tgccatttgt tgaagatttc ttttaactta gcgatattca
     8821 gctctttttg atagtgccat ttaggctgac cttcctgata ctgaagaccg atatgttcaa
     8881 actggaagac catagacaat tcttgcccct ttggatcaga gtagagcttg gcaatctctg
     8941 gtgttgctcc ccaagtctcc cctactgtca agagatcctt agctccaaag gtcgcctgat
     9001 tcatttcctt gagataggga tggagcatag gaccattatt gactaccttc tcgtcaggaa
     9061 ttttgccaat catgtcaata acatccatac ggaaaccacc aataccttta tcaatccaga
     9121 agttcatcat ctcataaatt ttctggcgaa gtttttcatt ttcccagttg agatccggct
     9181 gtttcttgct gaaaaagtgg agatagtatt gacctgactt ttcatcgtat tcccaagcag
     9241 acccactaaa gatagaatct aaatcgttag gctcatcccg ccagatatag tagtctcgct
     9301 cagggctatt aggattttca caggcctcta caaaccaggc atgctcatcc gaggtatgat
     9361 tgaccaccaa gtccatgatg atacggatat cacgcttctt agcttctgca atcagctgat
     9421 ccatgtcctc catggttcca aaaatagccg caatcgcttg ataatcagca atatcatagc
     9481 cattatcatc catagggctg tcataaacgg gagaaagcca aattgctgtg attcctagct
     9541 tggctagata gtccaactta ctggtaattc ctggcaaatc accaattcca tcttcattac
     9601 tatccataaa actctttgga tagacttgat agactacggc attatgccac catttttctt
     9661 gcatcttctt acctcattat tttaataatc tatagtatat gatagcactt tttaaaattt
     9721 tgtgcaagcg tttgcttaat ctttttaatt tcttttttag ctccgtagtt tcctaacttc
     9781 caatttttta ttataataga gtccagaggt aaaaaaatga aaaaacaagc ttttagttct
     9841 gaacaatatt taaatctaca acgcgaccac atcttagagc gcattaacca atttgacggc
     9901 aagctttacc tagagtttgg tggcaaaatg ttagaagatt tccacgctgc tcgtgtcctt
     9961 cctggttatg aacctgacaa caaaatcaag ctcttgcaag aattgaaaga gcaagttgag
    10021 gttgtgattg ccattaatgc tagcaacatt gaacattcca aagcacgtgg cgacttaggc
    10081 atttcttatg accaagaagt tcttcgtttg attgacaaat tcaatgaatt agggattttt
    10141 gttggttccg ttgtcattac acaatacgct ggccaactcg ctgcagatgc cttccgcaac
    10201 caacttgaga aaaacggaat tgattcttat cttcattatc caatcaaagg atatccgacg
    10261 gatatggatc acatcatttc cccagaaggc atgggcaaaa acgactacat caaaaccagt
    10321 cgcaacttga ttgtcgtaac cgctcctgga cccggttctg gaaaattggc aacgtgtatg
    10381 tccaatatgt accacgacca aatcaatggc atcaagtctg gctacgctaa atttgaaacc
    10441 ttccctgttt ggaatcttcc ccttcatcat ccagttaact tggcttatga agctgccaca
    10501 gctgaccttg atgatgtcaa catgattgac cccttccatc ttcaaaccta tggagaaacc
    10561 actgtcaact acaaccgtga tatcgaaatc ttcccagtgc tcaagcgcat gttggaacgc
    10621 attctaggag agtctccata tgcttctccg acagatatgg gtgtcaacat ggttggtttc
    10681 gctattactg atgacgaagc ggctatcgaa gcttctaaac aagaaatcat ccgtcgctac
    10741 tatcagactg ttcttgattt caaagccgaa aaagttggcg aagctgctgt caagaaaatt
    10801 gagttgctca tgaacgacct cggcatcaca cctgcagacc gtaaggttgc tgttactgca
    10861 cgtcaaaaag cagaagaaac tggcggacca gccctagccc ttgaattgcc aactggggaa
    10921 attgtcacag gtaagaactc agaactcttt ggtcctacag ccgctgcctt gatcaacgcc
    10981 atcaagaaat cagctgacat cgctaaagaa gtaaaactaa tcgaacctga agttgttaag
    11041 ccaatccaag gtcttaaaat tgatcatctg ggtagccgca atccacgcct tcattcaaat
    11101 gaaattctga ttgcccttgc tatcacagct acagaaaatc ctgatgctgc ccgcgctatg
    11161 gaagagcttg gcaacctcaa aggaagcgag gcccactcaa ccatcatctt gactgatgag
    11221 gacaagaatg tccttcgtaa actgggtatc aacgtaacct tcgacccata ctaccaatac
    11281 gaccgcttgt atcgtaagta aagatttgaa cctctccact ctcggagagg ttttctctct
    11341 gtctcaggag ccggctttat gttataatga aagaagaaaa ctgaaaggat ttaccatgtc
    11401 aaaagaagtt attgttgaaa gttttgaact tgaccacact attgtcaaag caccttatgt
    11461 tcgcttgatt ggggaagaaa cagggccaaa gggagatgtc atctccaatt atgatattcg
    11521 cttggtgcaa cccaatgaag actctatccc tactgctggc cttcacacta tcgagcacct
    11581 cttggccaaa ctcatccgta cccgtatcga cggtatgatt gactgttcac catttgggtg
    11641 ccgtacaggc ttccacatga ttatgtgggg acgtcatact agcgctgaga ttgcagcagt
    11701 tatcaaggat tcgcttaagg aaatcgctga aactactact tgggaagacg tccctggaac
    11761 aaccatcgaa tcttgcggaa actacaagga tcacagcctc ttttctgcta aagaatgggc
    11821 gaaactcatt ctagaacaag ggatttcaga tgatgccttt gaacgtcatg tgatttaaac
    11881 acaaaaaagg aaccagtttt tttcagctgg ctccttcttt ttttattctc aaaattttgt
    11941 cttatactca atgaaaatca aagagcaaac taggaaacta gccgcaggct gtacttgagt
    12001 acggcaaggc gacgttgacg tggtttgaat ttgattttcg aagagtatta ggctttttca
    12061 cgaataacta aaccaccatc ttccatatct gcttccagat gtttggcatc taggtgatcc
    12121 aagtggaagt ctgtcacctt atcacgaatt tcttgttcaa ccacgcgacg gagagggcga
    12181 acacccatga cttcgtcata gccttcttct gtgatatagt ccttagccgc ttgactgact
    12241 accaaatcaa tgcctttctt agccaaggtt tggttgactt cagccaacat caaatccaca
    12301 atcttagaaa gatcttcctt ggtcaagtgt gagaactcga taactgcgtt aaagcggttg
    12361 aggaactctg gtcggaagaa tggtttcaaa cgatccatca attctggttt atccgcatct
    12421 tctgtcaagt tggcttcata accaaatcca gcatttgagg tcgcgataat gacagtgttt
    12481 ttaaagttaa cggtatttcc ttgaccatcc gtcaaacgac catcatctag gacttgaagg
    12541 agaagggtaa tgacttgagg atcagccttt tcaatttcat ccaagagaat gatagagtaa
    12601 ggattgcgac gcacacgttc tgtcaaggta ttgctattgt catcataacc cacatatcct
    12661 gctgttgtac caattagctt agaaacagct gtgcggtcac tgtattcaga catatccaaa
    12721 cggatgatcg catccttagt tccaaacata tcgagtgcca attgcttagc aagttctgtc
    12781 ttaccaaccc cagttggtcc tacaaagagg aagctaccaa ttgggcgatt gccttcatca
    12841 aaaccagcac ggttacgacg gatagcacga gctaccgctt ctaccgcctt gtcttggcca
    12901 attaccttat cttgcagacg atgagccata tctttcaaac gctcgatgtc tgacgctccc
    12961 atttgtgaca ctggaatacc cgtcattcgt tctacagatt cagccacatc gttgacactt
    13021 gcagtcactt tcatatcttc tgtatggttt tcgattttct tctccaattc tgcaatgcgt
    13081 gttttatagt tgagagctgc ttcaaaatct tctgcctcaa ctgctttttc ttgcttgtct
    13141 ttctccgctt caatttcacg ttcaacagca tgcacatctg ttacaggatg ctgagctgcc
    13201 aaatgagctg ccgttacatc gacaaggtcg atagccttat cgggcaaact acgttgagga
    13261 atatactgaa cagaataatc cactgctgct ttcaaaacct cgtctggcaa gatgacattg
    13321 tggtgttgtt gataaaggtc acgaattcct tgaaggattt taaaggtatc ctctgctgaa
    13381 ggagcattta ctttcacttc gttgaaacga cgagcaagag ctgcattctt caagatggtg
    13441 ttacggtatt cgtcttgagt tgttgcccca atcactgtca attcaccacg agagagggct
    13501 ggcttgagaa tgtctgcaag tcccttagat ccactatctc caccagtgct accagcaccg
    13561 agaatttggt gaatttcatc aaagaagagg ataatattcc ctgcttcttt cacttcattg
    13621 actaaatttt gaacgttttc ttcaaaacta ccacggtatt gagtcccagc ctcaagacct
    13681 gaaatatcaa tggaaataat ttccttattc ttaatagcag ctggaacatc gccgttcaca
    13741 attgcttgag caagaccttc gacaacagct gtcttaccaa cacctgcatc tccgaccaga
    13801 acaggattgt tcttggtacg acgtgagagg atctcagatg tttcttgaat ttccttgttt
    13861 cgtccgataa caggatccag cttgccctcg cgagcttctg ctgtcaagtt tcgccctagt
    13921 ttagcaagga caccatcttg tttcatacct gaagcctgtt gtggcatttt gccatcaact
    13981 tctacatttc ctggtaattg accagttgca cgatagtgag caaattcctc aggtgtgact
    14041 tcacgtccat taatcaagta acgacgattt tcagaactgt atccgcgcat accacccatc
    14101 aattggttaa ataaatcatc catgttgtta aaattattaa agttgttgtt catattcttt
    14161 acctcttttt gttatttatt attactgata ttgactatct ttgacctttg ttttaaaaaa
    14221 tttagactag ctaaatcgct actctacgta ctatttctgg tttttctttt tcaagcagga
    14281 gtagactatc tttacttcta aagatttcta gatgaaacat agacccacct ctttcttttt
    14341 tactttaaat aagcgttcta gtaaggcttt catttacctt acgttcataa tatactacat
    14401 tagtcagaaa aggtcaagga ttttgacttt gactgaccaa tattttttat actcttcaaa
    14461 aacctcttca aaccacgtca gctctatctg caacctcaaa accgtgtttt gagcagccta
    14521 aagctagctt cctagtttgt tcttgatttt cattgagtat aagtgttcac gtaaggtttt
    14581 catttacctt acgttcataa tatactacat tagtcagaaa aggtcaagga ttttgacttt
    14641 aattgaccaa tattttttaa aaagcaaaaa caccctgaaa aatcagggtg ccgttcttac
    14701 atcaaataca aaattgctag cgtcaggaga cttgctagaa gtcccactcc ccaaaagaag
    14761 aagtcaacct tccactcgct ccaaggattt cctttaccag acaatcctcc aagctcaaaa
    14821 tggtgatgca caggcgtcat acggaaaata cgtttgccac cagtcatttt gaaataactt
    14881 acttgcatca taaccgaagt tgtttcaaaa acataaacaa ttccgataat caagagagtc
    14941 cattcttggt ggagggccat agagatagct gccaacattc caccgagagc caaacttccc
    15001 acatccccca taaagacctt agcaggctta tggttaaaga caaagaaacc gagcaaacca
    15061 ccaatcatgg caagaatcac tagaaggata tccatctgac cttgcacata ggcaataacg
    15121 ccataggcag acaaactaat cacaacggaa atactagcca gaccgtcaat accatctgtc
    15181 aagttcactg cgtttgaaaa accaactagc cagaaaagag cgaagacaat atagaaaatc
    15241 cctagatgta cttgataacc aaagacagaa agcatatcgc caccacgctc atagaaaagg
    15301 tagaaaatga cgccacctag aagctgaaga gccaatttct gtttgggatt aaggccctca
    15361 ttgattttac ggaagacctt gaggaagtca tctaaaaatc cgattaaacc ataaagaacc
    15421 aagataaata aaatcatacc aacattattg ctgagttgat tactaaatag ggcgaaaaag
    15481 aaagcaacta aaacagaagc aattaagaaa accaaacctc ccattgtagg agtcccagct
    15541 tttgcctggt gctgtttgac atcctcatgc atctgctggc ctgtaatttg cgcctttcta
    15601 taaaattgga taaaggctgg aattcctacc aaagttagta aaaatgtcac aattccagca
    15661 ctgatagaaa taaacatatt agtctcctaa agttaatgta atttttttaa tgtttttgat
    15721 agctgtatta gtccgaacat cttgcttctg aacgacggaa cctgaacctt caaattccaa
    15781 ctcaatatcc aaccatttag caaaggtttc agcagtctct tttttccagc catacatgtc
    15841 tggaatttct tctaccttat ccgataaaag gagaacttgt tggtttggtg caagattggt
    15901 cccttcttct acagaagtct ctttaatctt tgttccagta cctacaacga ttggttgcac
    15961 aatatttcgg cgtaaggctt ccgccaactc accaggtgaa atatccttga tgctaggcat
    16021 tgcataagaa gattctgtcg taactttatc taaatttttg gctggagatt gaagattgag
    16081 agattctttc atagctgaag cccgctccaa gattggggtg gcaaattctc ccaactggat
    16141 acctgaataa tgctcaggct gttgaaccgt tacatacaag ataaaatcag gattttcagc
    16201 aggattcata gtcacaactg agaaaatata attggtagaa ccaaccaagt atcctccatt
    16261 tttctcatca gcgatttgag ccgtaccgga tttaactgct acattttgtc caggaactgt
    16321 tataattggc tttcctgtgt agtgattata catagttcca tatagagggt ccgtcccaac
    16381 taagatcatg tgatttcgag ttgtgcttgc tgcctctttg gaaacaggat ttcctactat
    16441 ttctttttgt gacttacgta cagactgatt gttagtatca taaatagcac ttataaattt
    16501 tggctccagc ataactccat cattagcaat agctgtaaag gcacgaagca tttgtgtttg
    16561 tgtcactgaa attccttgcc caaatgagct ttgagcaata ctaacaatat tatcagctgg
    16621 aagttgacca gcgtattcat ctgtcaagcc aaagcgagtt ggaaccccaa atttaaagcg
    16681 ttttagataa tccaaccaag tagcatctcc cattttttgt tcaagtagac tcattccaac
    16741 attactggag tgagcgaaac cttgtaagaa agtcatcatc ccaccagtag tcaaaccttc
    16801 attaacatcc caatctcgag tcgtcgcatc cgctattttg aattcactgc tattgaagta
    16861 ttctccactt gggaaggtat tattatcaat agaagaagct aacgtcataa ccttcatggc
    16921 tgatcctggt tcatagttac tttgataaag aatatcacgc caaacaaagt cctcagtgat
    16981 tccttcttta gtatctgcat taaaggtagg tcgttgggtg gtagcgagga tttcaccggt
    17041 ctttgcactg accaaggtcg cggtcatata cttacctttt actttttcta gaaaggcatc
    17101 catctgagtt tccatgaaag attgtagcgg actagacaat gttgtataaa catccttgcc
    17161 atccacagtt tgttgcgata ccagttctgt acctggtacg atatttccta cacggtcttt
    17221 ttcataggta ataataccgt ctgtcccagc aagaatggta tttaaagaac tctccaaacc
    17281 agaagtccct aacaaactct tactaccatc ctcattttca tgaagttggg ctaagccaat
    17341 aaagctagaa gcaaattgtc cgtttgggta actacgattg ggactggttg taaaatcaat
    17401 ccccttgacc tctgcagttt ccaactcttt tttgatagac atcatattgg cataggtaat
    17461 cccattgccc tttgctccaa aggaaacttg cttgagatta ggttgcgaga gttgctctct
    17521 tacataagat tcttccatgt ccagatactt atgaaagacc tctgcaacct tgttaaattg
    17581 tgttttttct acgtaaagaa tcttacccgt tgctgactta tagttctcat caatgaccgc
    17641 atagacatta taggatgttg catcctcagc aatcgggact ccatttcggt cataaatagt
    17701 cccacgtttg gcaggaactg tacgggtggt ttgatgaacc ttcttagctt ccttcgctaa
    17761 atctgttcca aagcgagtgc ctgtcccaat aatgaccgca aaattgacta aaaaaatggc
    17821 aaaaacaaag acagataata aactcagact ttttccaact ctgcgtctgt tttcagccgg
    17881 cgatttccga tttttggtcg cataacggat tactcttttt gtccacttca tatcttactc
    17941 cgctattcta atattttcat tgtttaattg caaatcgtgt gaattggcaa tttctttcaa
    18001 acgttctgca cgtaatagtt cattgacctc ttgcttggca tcgtccaatt cggttttctt
    18061 ttcctctatc tgcgcattga tttttgtcaa atcattctgc acttgcaaga gcttggtctg
    18121 cataaaaata atactaatgg ctacaataag agtggttaca gcaatggaaa agtaaaaagc
    18181 tttttccaca cgcgaaaacc gtttaagttg catctgtagt atttgacctg ttttttccat
    18241 tttttctgcc atctttttcc ctcttacttg tgaatttttc tggccacgcg caacttggct
    18301 gagtgcgagc ggttattggc ttctaactct tccgcacttg gcaagattgg cttacgagac
    18361 accaattcca tcttgggctt gagatcatct gggatgaaag gcaagccttt tggaacttca
    18421 actgttgaag cttccttgaa caattgcttg gtcaagcggt cttctaagga atgaaaggta
    18481 atcactgaaa ttctaccatc cagagccaac atatccatag cctgctggat ggactcatct
    18541 gccgccccca gttcatcatt gacttcaatt cgaatagcct ggaaaatctg cttagcagga
    18601 tgccccttct tcttgagttc cttggcaggt ttgaccaact tgataatctc tgctaactca
    18661 gtcgttgtct caatcggctt cacttcacgc gcttgctcaa tcttacgcgc aatctgttta
    18721 gagaatttgt cctctccata cttgaagaaa atacgaacca agtcatgata gtcataattg
    18781 ttcaccactt cataggctgt cagactagca tcctgattca tccgcatgtc cagtggcgca
    18841 tcctttttat aagaaaaacc acgctcacgc tggtctaatt gaggactaga cactcccaag
    18901 tcataacaaa ttccatcaat ttcctgaaca ccagcttcgc gcaaacgtgc ctgtaaatga
    18961 cggaagttgt ccttgataaa ggtcaccatt cccttctcaa tgtaaggtgc caagcgtttt
    19021 tgcgcattgt caatggcatt ctgatcctgg tcaaaggcat agagatggcc tttttcactt
    19081 aatttactta ataaatactc gctatggcct gctccaccca aagtcgcatc aacgtagata
    19141 ccgtcaggtt ttacgtcaag catatcaatc gtttcgtgga gtaagaccgt tacatgatga
    19201 aattcttttg tcatatctta tctattttac cacaaatcca accagcttgc acttgtaaga
    19261 caaataggac aaggagacta ctatttcttc aaaaaatatg tcaaaaatac ttgacacttt
    19321 atgtttaaaa gactataata aagtcaaata tatacgacat aaatcagaaa ggagaacaga
    19381 tgaatcgtgt gaaagaattt cgcaaggaat tgggtatttc ccagctcgaa ctcgccaagg
    19441 atatcggtgt ctcgagacag actatcaata tgattgaaaa cgacaagtac aatccaacct
    19501 tggaactctg tctcaatctc gcccgcagcc tccaaactga cctcaacagt ctcttttggg
    19561 aggaagattt ttaaaaaagg agcaaactca tgaaaaaaga aaccttcact gaaaaactga
    19621 tcaaacgcac atacggtatt tctggtcccc ttgacgaata caaacggcgt gaggccgatc
    19681 gtattgggaa ccaagtcttt atcatcctct tttatctgat gattttcgga aatcttattc
    19741 cactccttct ggcctataaa taccctcaag aagtggctct aatctatcct cctctgattt
    19801 tagtgattgc cctcatcgct gctggctatg tcacctacca aatgaaaaaa acagggatta
    19861 cagctattga tccagacatg ctgagcgaga aagaaagtaa gcaactacat tacccaggtc
    19921 tgaaagcagg tttgttcttt ggtctatgga tgttttttat aactcctctt ctcaggatac
    19981 tcataggtga aggtcaggac tattttcatt ctcttctcac tataagaaat ggtgtatcaa
    20041 gcattctcgg ttctattttc ttcggagcga gcatacagtt cctcatctcc cgtcgcattg
    20101 caaaaactaa gaaaaatcaa gatgaggatt aggaggtgcc ctatgaaaaa agaaaagaga
    20161 cagttacgtt atcctggtct gaaagcaggt tcgatttatg gaacagtgat attttttata
    20221 attccactca ttgataccct aacaagtgaa aatccaaatt ttatcagttc tcttctaaat
    20281 acaaaacata tttttaaaac tatactggga gctttctttt tcggagtaat gatacatatt
    20341 gtcgactcac ttcgtattgc aagagctaaa aaagaccagg attaggaggt gccttatgaa
    20401 atcactagct agactactga tcattcatgt ttttatcagt attttccttt tcttcgccct
    20461 tatttcagga gctgtttctc atacagtttt actactccta ctcctctttc ttcctgcgct
    20521 caataaagga cttgagaaaa tacaatcaaa acggatacct gtcctcaacg cagccctctt
    20581 ctttctcctc atatcctttc cacaactttt aaccaaccct gtccaatgga aattttcaat
    20641 attcctagtc gtaaccatca tttcaagttt ggcctacttc tataactttt atcaagtagt
    20701 taaagaagta gatcaaaaac agttgattta ggagcttgct cccataattt ctcatttcat
    20761 aggcaaataa tcccacctag cttgttctag gtgggattat tttatttctt aatagagatt
    20821 gcaagtcatt ttcctttatt ttagaaagtc gactctttcc aattcacact acaatccaaa
    20881 acttgttgcc tttcttcttg attgcggcct tcaatctggt caatcaaaat ctttgtagcc
    20941 tgttgtcctt cctcaaagga gggctgaacc agcgtcgaaa cacttggaga agaaaagcaa
    21001 gtccactccg tattgtcaaa accaatcaac ccaacttgcg gcaagttata attcaactct
    21061 ttgataacgg taaagactag aggtagggcc caacagttag ggataaatac cagagttttt
    21121 tcatcgggat cgatttcttt ttgtaaaaat tccttaattt gttccaaatt cgtatgctta
    21181 tcttcaatgg ttagactggc gtgacgcata ttagcatctg ttaaagcatc cacaaaacca
    21241 cttgcccgct caatccgagt actcaaacga ctcgtatccg ctgtaatcaa gagaaaatgc
    21301 tcataacctt tttcgataca ggactgggtc atgtcataaa cggcatcata gttattggtt
    21361 ttaacccagc tagtccggtg ttcatagagc tgactatcaa aaaagaccat tttcttcttt
    21421 ttctcatcga tgatacgaga atattttcgg aaattagagg tcggctgaat aataaagccg
    21481 tctactccca agagaagcat gctttcaata taccggtcct cactctcttg gctgtaatta
    21541 ctatttccta tcattacctg gtagccattc tggctggcga tatcctcaat tcccttaaca
    21601 atttggtttg agaaactgtt ggtaatatca ccgatcaaaa caccgattaa ttttgttcgt
    21661 ttggagttta agctacgcgc aacaatgctc ggtttgtaat ttgtttcatg aataactttt
    21721 tcaatctttt cacgtgtctc ttgggacatt ttttcatatt tcccgtttag gtaaaatgac
    21781 acggttgttt tcgaggtctg agccatttcc gcaatgtctt ttatggtcag tttcttctcc
    21841 aatctaaaac acctcctact aatacttatt ataacatatt cactattcaa aatgcctatt
    21901 tctagcactg caagcgaatc attctctcat ttattttatc atatactaga cattttccac
    21961 gcatttttgt tccgtcaacg aggcacaatt tttcacctac acaaatatcc tcgcttaata
    22021 acagaatcag cttgtctgtt tcgtcatttt tcacttcaaa agccagagca ttttccactt
    22081 cacgatcatc tgtctggtag acagaatgac ggatgatttc aaaactctca tgcgcaatga
    22141 tggtataatc cagcttctga ttctcaaaga attggcgctt ggtgagtttg ctacttcttt
    22201 caagctcatt gtatttagga gaaatgatgg tatcttccaa atcaaaatca acttcactcc
    22261 atagtctcaa ctgattgatt ttcccatctt gataggtcac atccttgtca aggataaact
    22321 gagtcaacgc ctcatgctga ccttgacacc tgatgtcatc caccaagaac cagacatcct
    22381 ctaccaacat gaggattttt cttctgtgaa gataaggcaa atcaggttct gctgaccaat
    22441 aagccccttc aatataatgc attccctccc tttctttatg gtgacaaaac agggagtgag
    22501 gatagtattc atattcccag gatcctgtga ttctttccgg agctttccca tctacaatgc
    22561 aggtcgaatg actccaagca ctctttaaga gataacgttc atatatctcc cgataagaat
    22621 aacgcccagc atctatgaaa ataggttggc cttgatactg taagcaaaaa ctattctcgt
    22681 cactatggct atgggcactt cctagcggac catttttgaa aaatagataa cgatgttcat
    22741 ccttaatgca gacatgtcca gagtcttcaa agatcatgga cttaggctgc caagctctcc
    22801 tttcaaattc ctgcagtcgc ttgacctttt ctcgccccag gaacaagatg ctaagcaaat
    22861 caactttaac atccagaccg ttaagaaggt cttccttgtt caaaaccaca gcagacaggc
    22921 tcaacatttc tgtcgtttct gtagaatcgc tatcaccaaa agccaaagtc cgtccatcta
    22981 agcctgtcat catttgaata taggtcgcca tcttttccag caactcttgg taactatctt
    23041 gcaagtctgg aagcaagaga cacaaatcca gcaaggcttt ataaacctct acatgataga
    23101 gaatcgactg ttcaaactgg cttccatctc ctaaaatctg tgtctcaatt tgctgtttca
    23161 actcctctga agcaaaatgg taagcttctt ctagatccat cttatctgaa aagaaatgat
    23221 agatagcaag catcggaatt gtttgtaaaa tcccccagtt actaagggtg tacttggcgc
    23281 gatagtagct tttcataaag tcaatctgct tttctagact gaccaaaatt ttctctagtt
    23341 ctttctcctc tagcaagtca aatttcaaga ggagcaagag tagtttcaac caagtaaagg
    23401 aacgaatacc cgtatccaag attctagtca tcaaggattg aggagaaaat tctctcacct
    23461 gctcaatcca atcaaataga aagaacttgc acttttgaat atagtcctta tctccttcta
    23521 ccagataccc tatcataaac tgcaagagat attcttgtcg attgagcata taagaccatt
    23581 ctggatcatc ttcaaatact tgatcccata ccatcggctg gatttgatgg atttttgaac
    23641 aaggctccat atcccaagga ctatcaaaca taaaacgatt gtccatcaag cgttcaaggg
    23701 aactcttgac tttctcatag tcttttgaac agtgggacaa gatataatca cgacattggt
    23761 ttccatcgac tctttcaaaa aattgtcttc tttcttcttt cattatctat taccagaaaa
    23821 agaactactt aaaaagcagt tcttttgtct ttcccattac actttccttt tctacataga
    23881 tgaccacacc ttttgcaatc tgcaaggaga ccaagtcatc tttgatagaa atgatttttc
    23941 catgaattcc agacaataac aacacttcat caccagatgt taaagaagct aaatactctt
    24001 gtcgttgctc catctgtttg cgaagcaact tttgctgacg aatagaatga aagcttgaca
    24061 gtaaaagggg actcactgcc aagacaatca ctattccata aaacaatgtt gtatccatta
    24121 agctataatc ttaagccagc ttccgataat tccgatgata actgttaaaa taacgagttt
    24181 atatgttgtc catttctttt ctttgatcaa gtagtaaact aaaagtgtaa atagggctgg
    24241 tagaagagct ggagcaacct tatcaagcat tccctgaata cttacgatac tttgtttagc
    24301 gtctgcttta acttcccctg cagcaaaggt aatcggcacc ataatcttaa cagatgtcgc
    24361 tgccaaacca gcaattacgg ttacaccgat aatattggca atacgagaaa tcgttgccat
    24421 ctgttcgctt agtttatcaa tcacagttgt tcctagtttg tatccataca gaccagttga
    24481 caatttaatc gctgttaaaa tcgtattcat cgcaaggaag aacaagattg gaccgacaac
    24541 caagccttct tgagcaaacg aagctgcgat ggttgagaac aatggagcta aacagaattg
    24601 agaaagagaa tccccaatac ctgccaatgg tcccatcaag gccatcttga tgctacgtgt
    24661 ttcttttgtt ggtcggccat tttccaacat tacaagatgc aagctggtaa taaaaggcag
    24721 gaagtgtggg ttggtattat agaattcaca gttttcttcc aaggcttggt agaaaccttc
    24781 ctgatcctct ccatagtgtt ttttcaaagc aggatacatc acattggcat atcccaaccc
    24841 ttgatagtta ctatagttaa atccattttg acaaaagaat gcccgcaaag acgttttaag
    24901 ataatcacgt tttgttaatt tgttagatcc agtcatcgtg tgcttcctcc tctaccacat
    24961 gatccgctgt ttttggcttg ttataaaatt caatcaaagc aaagatagta cctacaattg
    25021 caataccaat tgttgggatg tttagataag ctgcacaaac atatcccaac aagacaaagg
    25081 gaatcaactc tttcttagcc atcactgaca agatcatcgc aaaaccgata gctgggagca
    25141 ttttaccagc aactgtcaaa cctgtaagca atactggtgg aatgtagtct acgagtttca
    25201 acaaggtatc cattgaaagg gcacccaagc aacccaaggt aaatccaata aaggcaaaca
    25261 accaaattgt tgcatttaga gtgaacttga atttcttcaa attatggttt ttcaagtgct
    25321 tcatcgccgt ttcaggcgca ccagcacgta cagtgtaggc gaaagtttgt aagaattgaa
    25381 tcgccacagc aatcggagta gagagggcaa gagccgcttc tggactgact ttaccagcac
    25441 tagtgatagc catcaaggta ccaaagatac caggtccgat tgggtttggt ggaacagtac
    25501 ctccagcacc aacaccgaat cccatataag ccaattcacc aatagctccc attgcaagag
    25561 cagtaggcaa atcacctaga ataattccga caccaaatga cagaacaata catctattgg
    25621 tgtaaattcc taacagcatt ccactaaaac agaaagctgt ccataatcca attaaaatcg
    25681 cttgaaatac attaatcgac ataacgatct cctcttttaa atatagtcca taatgttgac
    25741 ttgaacagct ccatcatttc ctgttggagt tgttttcgta ttaaatgtta cttgatgagt
    25801 ttggctcaat tccttgaggg ccgccttgtc ctcttcaccc aggaagatgg agcgtgtcac
    25861 ttgctcttta ccagtagcat tgtgaatgtt cccaatattg atttctttga tagggacacc
    25921 accttctacc aaggttaaag cgtccttcac atcctttaca acgataaaga tcgtttgagc
    25981 aggattagcc ttgtgaatga tatcaatcac cttttgcaaa gggaagaaac gcatggcaac
    26041 tgagtctggc acaactgttt tcatcagagt ttgttgcatc ttgtctgtgc ttacttcgtc
    26101 attggcaaca atgaccgtat tacaacctag gtattttacc caaagttgtc cttgcccatg
    26161 aatcaaccgt tcatcgatac gggtcataat aatatttggc attgtcatat ttctcctcct
    26221 accaatatag gttccagtct ttgtagaaac ggataagggc ttctaggtaa tagtagtcac
    26281 cccagatatt gccttcatcc actcctttac ctgaatgcca tgagtacaca ccgtggagga
    26341 gacttgtccc accaggggta aattgatcat ttgcataatg ttcgatcaag gaacgaagca
    26401 tggcatgcat agcatgttta taaatatctt tgtcagcatc cacctctggg agatgtttta
    26461 gcatttcatg aatcccacag acggcgatag ctgttgctga agaatctcgt gattgagcac
    26521 taccatcatt aaaaatcaaa tcccaatagg acacatgatc ttttggcaga cgattcaaga
    26581 aataattggt cacaccctta aacaagtcaa agcaggactc gtcttttaag tgacgataag
    26641 tcaaaggaat accatagact ccccatgatt gaccacgtgc ccagcatgaa tcatcactat
    26701 acccttgtct cgttacacct ttaaagggtt gacctgtctc aggatcaaaa tagaaggtgt
    26761 ggaaggacga agcgtcatca cggattacat tattagctga agcatagaaa tgactttctg
    26821 caatatcgta gtatttttga tcgcctgttt cttgataagc aaagaataag agttggatat
    26881 tgagcaagca gtcgataatc aaacggtaat gctctttctt gcccaagtct ccccaagctt
    26941 gaataaaacc acctttttct tgatagcgtt caatcaactt atctgcagct ttcaaggttg
    27001 cttctctagc ctctccatct ccatttatct tatattcagc catacaagac ggtgtgtaca
    27061 agaagccgag atcatggtga tccaattcta ctctcttatt gacacgatcc aggaaagaaa
    27121 gaacattttt atgagcgatg tttttaaatg catcctgttg actgtattca taagccaacc
    27181 acagttctcc tgtccagaaa ccattggtcc attccgtgtt atccatgatt ggatagacat
    27241 tatcaaaggt agctggcgtc gggaaatctt ccttgaaata gtcaaggttg agttctaact
    27301 gccgaataac cttatcgatt gcctggccga cttcttcttt cgtcagaagt ggtacttcta
    27361 agaagcgctc aggcgatttt attttttcaa tcgtaacctt ttttatcatt cttttaccct
    27421 ttaaataccc gattcgaaat cttcttcctc ttcctctgca tccgttgaaa acaattcttt
    27481 accatttacg actccgccct gggccgccac tactgattta ttaacaacct catcaaagat
    27541 atgagccatt ctagcaaaga ctgcttccat taacatggct aagttcaaac ccgaaagaac
    27601 attcattgtt ttggctggat tttctcccat tatggtagaa gaaaccttga atggcgatcc
    27661 tcccaagaga tcacttagga ttaaaacttc ttcttcattt gaaattgcaa gtaagatttt
    27721 ttgcttgagt tcatctgctg gcattccttc cacaaagtca attgcctcca cattttcttg
    27781 attgcctgca atcaattgta aagaactata aatccctgta gcaaaatgtc catgccctac
    27841 aagtacaatt ttcatctttt ctcctttctt gtttaggttt accggtttac tttatattcc
    27901 cca
//
LOCUS       DAVLVZ010000023        26383 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000023 DAVLVZ010000000
VERSION     DAVLVZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26383
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.023"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            83..153
                     /locus_tag="VYN77_000987"
     tRNA            83..153
                     /locus_tag="VYN77_000987"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:115..117,aa:Cys,seq:gca)
     gene            complement(351..1682)
                     /locus_tag="VYN77_000988"
     CDS             complement(351..1682)
                     /locus_tag="VYN77_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1558464.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(1911..2735)
                     /gene="endA"
                     /locus_tag="VYN77_000989"
     CDS             complement(1911..2735)
                     /gene="endA"
                     /locus_tag="VYN77_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1558465.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(2774..2962)
                     /locus_tag="VYN77_000990"
     CDS             complement(2774..2962)
                     /locus_tag="VYN77_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1558466.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(2955..4238)
                     /gene="murA"
                     /locus_tag="VYN77_000991"
     CDS             complement(2955..4238)
                     /gene="murA"
                     /locus_tag="VYN77_000991"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1558467.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(4262..4492)
                     /locus_tag="VYN77_000992"
     CDS             complement(4262..4492)
                     /locus_tag="VYN77_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1558468.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(4550..5587)
                     /locus_tag="VYN77_000993"
     CDS             complement(4550..5587)
                     /locus_tag="VYN77_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1558469.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(5571..6059)
                     /gene="coaD"
                     /locus_tag="VYN77_000994"
     CDS             complement(5571..6059)
                     /gene="coaD"
                     /locus_tag="VYN77_000994"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1558470.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(6049..6588)
                     /gene="rsmD"
                     /locus_tag="VYN77_000995"
     CDS             complement(6049..6588)
                     /gene="rsmD"
                     /locus_tag="VYN77_000995"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1558471.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(6653..7645)
                     /locus_tag="VYN77_000996"
     CDS             complement(6653..7645)
                     /locus_tag="VYN77_000996"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1558472.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTSKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(7757..8440)
                     /locus_tag="VYN77_000997"
     CDS             complement(7757..8440)
                     /locus_tag="VYN77_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1558473.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(8496..9236)
                     /locus_tag="VYN77_000998"
     CDS             complement(8496..9236)
                     /locus_tag="VYN77_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1558474.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSPALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            9270..9548
                     /locus_tag="VYN77_000999"
     CDS             9270..9548
                     /locus_tag="VYN77_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1558475.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            9626..10552
                     /gene="yidC"
                     /locus_tag="VYN77_001000"
     CDS             9626..10552
                     /gene="yidC"
                     /locus_tag="VYN77_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1558476.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVHEELAKNPSKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(10627..11421)
                     /gene="pflA"
                     /locus_tag="VYN77_001001"
     CDS             complement(10627..11421)
                     /gene="pflA"
                     /locus_tag="VYN77_001001"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1558477.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(11547..12797)
                     /locus_tag="VYN77_001002"
     CDS             complement(11547..12797)
                     /locus_tag="VYN77_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1558478.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVASIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(12864..13691)
                     /gene="purR"
                     /locus_tag="VYN77_001003"
     CDS             complement(12864..13691)
                     /gene="purR"
                     /locus_tag="VYN77_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1558479.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(13958..14899)
                     /locus_tag="VYN77_001004"
     CDS             complement(13958..14899)
                     /locus_tag="VYN77_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1558480.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(14889..16145)
                     /locus_tag="VYN77_001005"
     CDS             complement(14889..16145)
                     /locus_tag="VYN77_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1558481.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(16147..16809)
                     /locus_tag="VYN77_001006"
     CDS             complement(16147..16809)
                     /locus_tag="VYN77_001006"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1558482.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(16772..17428)
                     /locus_tag="VYN77_001007"
     CDS             complement(16772..17428)
                     /locus_tag="VYN77_001007"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1558483.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(17439..18317)
                     /gene="rsgA"
                     /locus_tag="VYN77_001008"
     CDS             complement(17439..18317)
                     /gene="rsgA"
                     /locus_tag="VYN77_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1558484.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHIPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(18319..19191)
                     /gene="rsmA"
                     /locus_tag="VYN77_001009"
     CDS             complement(18319..19191)
                     /gene="rsmA"
                     /locus_tag="VYN77_001009"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1558485.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(19322..19630)
                     /locus_tag="VYN77_001010"
     CDS             complement(19322..19630)
                     /locus_tag="VYN77_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558486.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(19623..20264)
                     /locus_tag="VYN77_001011"
     CDS             complement(19623..20264)
                     /locus_tag="VYN77_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558487.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(20266..22296)
                     /locus_tag="VYN77_001012"
     CDS             complement(20266..22296)
                     /locus_tag="VYN77_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1558488.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMTN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GARPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRDLMIKRLTGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(22565..23464)
                     /locus_tag="VYN77_001013"
     CDS             complement(22565..23464)
                     /locus_tag="VYN77_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1558489.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQGLTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(23536..24096)
                     /gene="rnmV"
                     /locus_tag="VYN77_001014"
     CDS             complement(23536..24096)
                     /gene="rnmV"
                     /locus_tag="VYN77_001014"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1558490.1"
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(24096..24869)
                     /locus_tag="VYN77_001015"
     CDS             complement(24096..24869)
                     /locus_tag="VYN77_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1558491.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(25175..25840)
                     /locus_tag="VYN77_001016"
     CDS             complement(25175..25840)
                     /locus_tag="VYN77_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV1558492.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKLENTPGGDKGGNTGSSD
                     ANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKNKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRPVAPNAA
                     PKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTR
                     RRK"
     gene            complement(26103..26237)
                     /gene="rpmH"
                     /locus_tag="VYN77_001017"
     CDS             complement(26103..26237)
                     /gene="rpmH"
                     /locus_tag="VYN77_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1558493.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
BASE COUNT         7500 a         6222 c         4940 g         7721 t
ORIGIN      
        1 acattcgaat cacgctatgg tacaaaaaac tgaaaaaact attgactgaa aatcattttc
       61 aaggtataat aataaacgtt aaggcggtat agccaagtgg taaggcacgg ctctgcaaaa
      121 gcttgatcgt cggttcaaat ccgtctaccg ccttctataa cttgatttat caggtttcaa
      181 atgaacagaa agcccaattt gaagggcttt tcttattttc cctcgaataa atacgtataa
      241 ctttaaaaac ttttggagcg agtttgtggc agagttcttt ccatggcata attccctttt
      301 gaaatcaatt gaaaaaagct tcgtcacgcc tggtgacaaa agcctctagt ttattctgtt
      361 tcttcttcta tttcgacttc tgtgatttgg acttttacct tggtaacacg tccattcttc
      421 actttatcat tggttaagat aaactgtttg ttttggctga ccaattcata gctgagtttc
      481 tcagtcgttg gaatggttcc cactcctgtc aaataataac cagcgatagt atcaacgtca
      541 tcactttcca gttcaacatt aaagtaatta ttgaaatcat tgagggtcat agttccttga
      601 acaatgtagg tatcctcgcc aatctgatga acatctattg ctgctttgtc cgtttcatca
      661 tcaatttcac caacaatttc ctctaagagg tcttccaaag tcaccaaacc agccatacca
      721 ccgtattcat ccagcaagat tgccatttgt ctttgggtat ttcgcagttc ttttagcaag
      781 tcatccacaa aaatagtttc aggtacaaaa agtggatctt gtaaaattct cttccaaaca
      841 atattgtcaa aaccgtccac aaagcctgcc ttaaggagac tcttggtgtg aatgattcca
      901 attacattgt ccttatcccc atcataaacc gggatacgag aataattttg ttttaaaata
      961 ctttggataa tggcttgact atcatcctga atatccacca taaaggcatc cgttcgagga
     1021 accataacct ctcgtgccat cagttcatcg agcgaaaaga caccttgtag catctcaatc
     1081 tcatcagcat ccaatgtttc ttcactattt gtcagcatat aggcaatttc atcacgggtc
     1141 atcttttcat ccgcatcatc gaatgacata ggagtcaaat ggctcaagaa attggtcgaa
     1201 gcagctaaaa gccaaacaaa aggactgact agttttccga tcccaatgat aaccggcgct
     1261 gtacgaattg ccaaggcatc ctttagatta agagcgattc tcttaggata taattcccca
     1321 aaaacgatgg aaatataggt caaaaatgcc aaggatagaa aagttgccac ggcttgtgct
     1381 gtttcgccat tcccaagcca agaggcaatc acacgtccta gagtatcagt taaactcgcc
     1441 cctgataaga ttgtaatcag ggtgattcct acctgaatgg ttgataaaaa gtggttagga
     1501 ttttctagta ccttcagcag gcggatgtag cgtctgtctc cttcttccgc cttttgttca
     1561 actcgggcac gattaagaga aaccatggcc atttctgtgg ctgagaaaaa ggcatttaat
     1621 accgtcaaga taaacaataa aacaaattgt agcaacaaat tctgactact tgggtcttcc
     1681 atagttcttc tcctaaaata gtatcttatc ccttattata tcacaaaatt taatccaata
     1741 catattattt tttcttaccg taacttctca aagtaacttc catctctctc ccaaaactgg
     1801 aagttagttt tagaagttgc tattttttct tggtacctgt tctagccctg cttaaactag
     1861 taaaaagaac tcctacatcc ataagtgaca taggagttta ggcttatctt ttactgagtt
     1921 acagttactt ctccagttcg gtaatccagt tgaagtccct tttgaacatt gggaactaga
     1981 acattgaatt ccaattctcc atccgaagac ttggcttcaa tctgtgaagc tgagggaact
     2041 aaatcctcgt ttgaagcgta gtaaagggtt acacggtaac ggacacgctt gttttggtcc
     2101 aaggctttac gcaccttgct ttcatagtag ttttgaccag tcgaatactc ggcttgtgcc
     2161 tgatttgccc aggctgtctg aacagcaatg tttttaggat tgcttgttga ggcatcaaaa
     2221 ccatccaaac caccgattaa ggcatagcct aacaaatgac ctctatcgac tgcatgggta
     2281 taagagccct ttagattctt gacctgatgc caacctggag gagtccaaga agttgaacca
     2341 ttcccagttt ctttacgatt cttgtactga cgagtggcct tagacaagag ggcattagct
     2401 acggttggaa cagtttcctt gcccactgtc tttgttttat tgtcagcgta gggcttactt
     2461 gaaaccttgg catctagatt tgttttatta ccattgacga taaaagcacc tgagccattc
     2521 cactccagac tcccctttat ttgactcttg actgcgtctg ttaagacact ctctgccaat
     2581 gcttgactag gagcttcaga cgcttgtttt ttctgactaa gattggtttt gggactatta
     2641 ggtgccgacg gcatctgctt gatataatag ctccctgtag acaaaagcaa taacactagc
     2701 agtccgatta gtgtctgtct tgtttttttg ttcatatttc tccttatctt tagaaaaagg
     2761 ctggttctcc agcctaattt cctgtaaatt tatgaatcaa ttcctgccat tttgctggag
     2821 acaggatagc ccatggatct tgacccttgc ccaagattcc ataacctacc attaaaccga
     2881 ttcctagagc cagagtacct aaaatcagta ctatgatgac taaaagtaaa cgcttgacta
     2941 cgtagcttga tttcttattc atcttcatca cttgcctcaa tccgctgaat cttagcacct
     3001 agctgcgcca acttctcatg gaaaccgtag taacctctat ccaagtgaac caatttaccg
     3061 accacagttt ctccctgtgc taccaaacct gtcaaaatca aggctgcact ggcacgaagg
     3121 tcagttgaaa gaacttctgc tccctgcaaa ggctgtccac caacaatacg agctgtatca
     3181 cggataatct cagaatgcaa gcccatgcgg cgcatctctt ctaggtgttg gaaacgattt
     3241 tcgaaaactg tctccaccat ggttgattcg ccttttgcaa ctgtcatcaa ggctgtaaat
     3301 tgagcctgca tatctgttgg aaatcctggg tggggcaagg ttttcacatg aacagctttt
     3361 agattttcta gttgagaacg aacacgaatt ccttcgtctt cttcaattac ttcaacaccc
     3421 atttcaagta acttggcaat caagggacgg ttgtgctccc agacagcgtc tcgaatcaag
     3481 acatcaccac cagtcatggc agcagctacc ataaaggttc ctgcttcgat acggtcttgg
     3541 actacattgt gagtcgtacc atgaagtttc tcaacaccag taatggttat agtctctgta
     3601 ccagcacctt tgaccttggc tcccatttca ttaaggagaa tggctaagtc aacaatctca
     3661 ggctcacgcg cagcattctc aatcactgtc accccatcag ccagagtcgc tgccatcatc
     3721 aagttctgcg ttgcaccaac acttggaaag tccatataga tatgagcgcc atgcaagcgt
     3781 tctgccttgg cttcgatgta accagctgtc tgactaatct taacccccat agcttccaga
     3841 cctttcaaat gaagatcaat aggacggcta ccaatcgtac aaccacctgg catggatacc
     3901 ttggcatgac ccacacgggc aaggattggc cctaatacaa cgatggaggc gcgcatcttg
     3961 ctgacatact tgtaaggggc ttcctcagtg atgtcgccag tagcatccac cttgacaaga
     4021 tgagcttcct catcaaagtc aaccttggca ttcaaaccac caactacctg attcataata
     4081 aagacatccg acaaaatcgg aacattctgc aagacggtct ttccttcact tgctagaata
     4141 gtcgctgcca acaagggtaa gactgcattt tttgctccct cgatcgtcac gcttcctacc
     4201 agacgattat cgccaccttg aaccacaatt ttatccatac tcgtttcctt tactcttgat
     4261 ttcataataa tcctctaagc gcttcttgcc acaaatgata caggctaata aagaaagaac
     4321 tcaggatgta gcctatgaca atgctaaaaa aggctactaa taaacgaact tttcttgtat
     4381 tatcatctgt cacttttaaa accttctccc atctaacaag atcctttaaa aggtaaaaac
     4441 tcacataaat aaagaacata tgactactta gagtgaataa taattgaacc atttgactat
     4501 tataccatct tctctgtttg tgcgctagtt tggaaacttc actcaaaaat tagggattgt
     4561 ttttcaagta atcaatcgca tcttgtaggg ttttaacggg cacgattttc atatccgtct
     4621 tgattgtttt agccgcttct agggctgttt ggtagttgtt tttcgcgtcc ggatgcgcct
     4681 tttgttcttc ttcgctaaca gggttatcag gagcaaagaa aatagcagca cctgccctag
     4741 ccgaagctac aaccttctta tcaatgcctc caatgtctcc cacattacca tcgcggtcaa
     4801 tggtacctgt accggcaaca atacgaccat tacgaagatc tgggtgagct atttgagtat
     4861 agatagctag actaaacatg agaccagcac ttgggccgcc aataccagct gttgaaaagc
     4921 taattgggac attgctgatt acctctgtac gatcaatcaa gccgattcca attccatttt
     4981 tgccattttc caaggtgata atctttcctt ctgcagactt ggtttgccca tcctcttcat
     5041 aggtgacctt gacggaatcc cctaattttt gagaactgac gtaatcaatc aagtctttgg
     5101 aactatcaaa ggtctgatca ttgactgctg tgactgtatc agagatattg agaatccctt
     5161 taaaggttga attatccgtc acattcaaaa cataaactcc aaagtacttg agttcgatat
     5221 ccttaccagc tgtttttagt ccttgatact tggccatatt ttgcgatgtt tgcatgtaga
     5281 attgattgat tcgcataaat tcaacatcgg aagaaccacc tgtagtctcc tgagcactac
     5341 gaatatctgt aaaaggtgtc aaccaagcat aaatcatatg agctaaagtg gcatgttgaa
     5401 caccaaccgt aacgaattga taggcaccag cttccttatc ttctgtgtca tttactttaa
     5461 ggacttggcg aatatcttcc gaaccacctg gaacctctat ataataaggc aagggcacta
     5521 caaatgccaa gaaagtcaca atcaaggccg caatgacgta taagggccat ctaatctttt
     5581 ttttcatttc ttatttcctc caaaatactc tcgggaacat ggcaggcaat atcctgacca
     5641 aacttcaaaa gctctcgaac acctgatgaa ctgatataga gatgttcagg ccggctatgt
     5701 aaataaatag tctctatatc agaagacagc tgatgattgt agtaatcaaa actagcttca
     5761 tattgcaaat ccgccgcatt cctcaacccc cgtactagga aagtagcccc cagtcttttt
     5821 gcaacatcga ccaccaattc atcatgagaa gccacgactt caacattttc caaatgtccc
     5881 aaagcctttt ctagcccccg tttacgattt tcaagaggga gaaatccttg tttgtgggga
     5941 ttaaaaaaaa tacccacata aagcttatca aaaagtctgc tcgcccgttc aatgatatcc
     6001 agatgcccat ttgtcatcgg atcaaatgag cctgtgaata agccaatctt atctgacata
     6061 gactgtcacc ttactaattc cataaatctt ttccttccag atacccagac aggcaatctc
     6121 ttctggaagt tcaacggcct tatccgtctc gcacacaacc ataacatctt cagaaaaaag
     6181 ctctctctta gccatttttt caatatctgc tacgatttgt tccttggcat agggagggtc
     6241 taagaaaacg aggtcaaatt ccccagatac ctgttccaat gccctctctg catccatctt
     6301 gaggagttga aattttccaa cttccttggt catctggata ttttcagcca cgatggtctg
     6361 agccttacgg tctcgctcca ccaaaacagc actggacatg ccacgcgata ctgcttcgat
     6421 agataaacca ccactacctg cataaaggtc caagactcgt cccccttcaa agtagggacc
     6481 aatcatgtta aaaatggctc ccctaacctt atccgaagta ggtcttgttg tcttgccttc
     6541 tagtgtcttg aggggacgtc ccccatagat tcctgatacg attttcatac tgtttattat
     6601 accaaattat agacaaaaag agaaagaaaa ccgaaccttg cggttcgatt ctctacaaaa
     6661 tattttcgta agtatcgcgt acttcttgag gccaaacact tgtttgcact tctccgatgt
     6721 gtctcttgcg aagtaggaac atggccatac gagattgtcc aattcctcca ccgattgtca
     6781 atgggaatag gccattcaac aaagacttgt gccattccaa ttccaagcgg tcttcatcac
     6841 ctgtaatttc cacctgacgt ctaagagttt cttcatctac acgaattccc atagaagaca
     6901 actcaaaggc tccacctaaa gactcattcc agacaagaat atcaccattt agacccttgt
     6961 agccattctc agactcgctt gtccagtcat catagtctgg tgcacgtcca tcgtgcggtt
     7021 taccatctgg caactcgcca ccgataccaa tcaaaaagac ggctccaaat tctttacaaa
     7081 tcgcattttc acgttctttc gatgtcaagt ctggatagcg ttctaccaat tcttctgtat
     7141 ggataaaggt gatttgtttt ggcaagatag actcgatgtc atagcgagct tcaacagcta
     7201 gctcagtcag gcgaatagcc ttataaatct tctcaactgt ttcttttaga taaacgatgt
     7261 tacgcttacc atttgggata accttctccc agtcccactg gtcaacataa acagagtggg
     7321 ttgcatccaa ggaatcctca tctggacgaa gggctttcat gtggacaaag agaccctctc
     7381 cttcaccaaa gccaaaacga gccaaggtgt ggcgtttcca tttagcaagt gagtgcacca
     7441 cttcataagt agcatcaggg atttggagaa ccttgaccga tactggattt tccacaccag
     7501 acaggttgtc ctgcattccg tcaccgacct tactcaagat aggaccttga acttcgacaa
     7561 cttctagctt atctttcaaa tactgggtaa aagtgttttt gacaaaggaa atttcttctt
     7621 gttgatggat aaaacttttc ttcatagaca actcctcaaa aatttaataa acgtattata
     7681 cacctatttc caagaaaaga aaagagaaaa tttaaaaaaa atcagtgtca ctcaaaacac
     7741 tgatttcata gaccttttag tcattgcgac caaagatacg tagaatactt aggaaaagat
     7801 tgataaaatc aagatagatg ctgagagcca ttgacaccac ccagcctgtc gctacacgac
     7861 cttgtgattg ttcataggca aggcgaatct tttggttatc ccaagcaatc aaccctgaaa
     7921 aaaccaaaac catggctaca ctaatcatat aatcaaagaa gccactagcc aagaaaatat
     7981 tgactaccat agcaatcagt aaaccaatga gagctgccat cattgctcga ccaatcccac
     8041 tcaagtcttt cttggtaaag ataccaactg ccgccatgac aaagaagaga agggcgctcg
     8101 atacaaaggc agataaaact gtacccggag tatagaaagc caccacaaaa ctaagggtaa
     8161 agccgtttaa aacggagtaa agtaaaaata ctggaagagc agctggacta tttcttgaag
     8221 ccatgctact ggcaacgaaa accaaagcca actccgcaaa agtagcaatg gtcaaccaga
     8281 gacgcccctg catcaacaag taaaccaact gagactgaaa gaccgtcaac ataaggcctg
     8341 ataccaaagc agatagtccg attcccagac caacaaaggc ataaacctta gcgtaaaatt
     8401 gattgagacc tgcacggtca tgtataatag tgtgattcat ccttttctcc ttttctgtga
     8461 acatctttcc ttgattataa caaaaaaagt taaaattagc ttaaatggaa gattaaaata
     8521 ccggctgcaa ctgcaacatt caaactctcg gcctgcccct tcatgctaat atggaccaac
     8581 tggtctgcac tttcagccat aaggggacta attccttgac cctcatttcc cataactagt
     8641 acaaaatttt ctatagaagg cagttctctg taatcaacag aatctttaga tagggttgtt
     8701 gctagcactg ggatagctac ctttttagtt tcgtcaagaa gcgctggact agtcatccgg
     8761 taaatgggca gatggaaatg actaccttgc atggaacgta gggttttgag actgtagatg
     8821 tctgccgact tatctgaaac aatcactcct gtaaaacctg ctgcatccgc agttcgaatg
     8881 atagttccta cattaccggg atcttgcaca tcttccaaaa acaagaactt gccctgactc
     8941 aaatcagctt gtcctacttc ttctttttga accaccgcca caattccctg tggagtctga
     9001 gaatccgcca aatctagcaa aatatcctct gaaatccaga cagtttgcgg aaaagcagct
     9061 aactgatctc gataattttc tagggcaaag attttctcaa tcgtcactcc agcttggaca
     9121 gcttcttcaa acaagtgcca gccttcaatc aaataggcag acttgcggta ttttttttgg
     9181 tgtaatttct tggcattttt taccacagaa ttggcttttg aggttataat agtcatagaa
     9241 atattataac acaatcaaag ggggttggta tgcaaaaggt tagaatgatt gcccaaggta
     9301 gggtacaggg agtcggcttt cgttggggtg tttacagctt ggcacttgaa attggtggca
     9361 tcacaggtcg agtatggaat aacgacgatg gcacagtgga aatcttagcc caagcagact
     9421 catctgctat catggcaaaa tttatccaag aaatccgaaa aggaccgaca cctttttcaa
     9481 aagtaagcta cttagatgtc aaactaagca actttcctcc ctactctgac tttaaaatcg
     9541 caaattaggt ctccagaact attgtatatt ttgtaaaaaa acagtagaat agaaaggtat
     9601 aatttttaaa gaaggaataa aaacagtgaa atctattaaa cgttttgcac tctcagctat
     9661 gggagcggct atgttgcttg tcttgactgg ttgtgtcaat gtcgataaaa ccacaggtca
     9721 gccaacagga tttatttgga ataccatcgg agcccctatg gctgaagcta tcaagtactt
     9781 cgctactgat aaaggtctag gctttggtgt cgctatcatc atcgtaacca ttatcgtgcg
     9841 cttgattatc ttgccacttg gtatctacca atcatggaag gcaacgcttc actctgaaaa
     9901 gatgaacgcc ctcaagcacg tccttgagcc acaccaaacg cgtctcaaag aagcgactac
     9961 tcaagaagaa aaactcgaag cccaacaagc tctctttgct gctcaaaaag agcacggtat
    10021 cagcatgttt ggcggtgtag gatgtttccc tatcctcctt caaatgcctt tcttctctgc
    10081 tatctacttt gctgcccaac atactgaagg ggttgctcaa gcaagctacc taggcattcc
    10141 tctaggttct ccaagtatga ttttggttgc ctgtgctggt gtcctttact atcttcaatc
    10201 gctcctttca cttcacggag tagaagatga aatgcaaaga gaacaaatca agaaaatgat
    10261 ttacatgagc ccactcatga tcgtcgtctt ctccctcttc tcaccagcta gtgtcacact
    10321 ttactgggtt gtcggtggtt tcatgatgat tctccagcaa tttatcgtca actatatcgt
    10381 tcgtccaaaa cttcgcaaaa aagtccatga agaactggca aagaacccat caaaagcaag
    10441 tgctttctca acaccaagtg gacgaaaaga cgttacccct gaacaaccaa ctgctattac
    10501 aagcaagaaa aaacacaaaa atcgcaacgc tggaaaacaa cgttcgagat aagaagaaaa
    10561 aggcttagct gatttgctaa gtctttttgc aagtcaaaag cccgatgttt ccatcaggct
    10621 tcttttctat ccatgtacac gtttcatata atcttgataa ctttcggtat ccatgagttt
    10681 tttagcgttc ttgacgcgat ctgctgttgg tggtttgact ccttcgaggg aatatggaat
    10741 tccaagttca cgccacttga actcacccat ggtgtgataa ggtagaattt caaacttatc
    10801 aacatttttg agggtcttga cgaacttacc aagttcaatc aagtcatcat ctctgtctgt
    10861 caatcctgga actagcacgt ggcgaatcca gacaggtttt ccaatatctg atagatactg
    10921 ggcacaagcc aagatatttt tattggtttg gctagtgaca atcttgtgct gttcttcgtt
    10981 gatttccttg atatccaaaa gaaccaagtc agtgacagcc atgagtttgt caaacttctc
    11041 aaggtaacgc ggtttattac ggaaaggaag agcacaggtg tccaaggtac agtggattcc
    11101 ttgttcctta gccttggtga agagagcaat caggaaatca atctgcaaga gagcttctcc
    11161 tccactgact gtaatcccac ccttatttcc ccagaaacca cggtagcgca aggcctctgt
    11221 caagacatca tctaccgtcc gttcacgtga cttattggac tccatagccc aagtgtctgg
    11281 gttgtggcaa tactggcaac gcatgtgaca gccctgcaaa aagacaataa agcgaatacc
    11341 aggcccatct actgacccaa agctttctgt cgaatgcacc atccctgtca cttgtccata
    11401 atcaattgtt tcttcagaca tatcgttacc ttccttgaaa acgttttata gttttattat
    11461 atcacgactt gaaggaaaaa caagattttt gcctattttt tagaaagcaa tctgtttttc
    11521 aatttcattt tcaatctatc atcagattat tcttcaaaat caaagccata taaggttgca
    11581 aaatagtcct cggggcgctc tgcacggcgg atttgacggg ctttaccttc ttcggtatag
    11641 aggatttccg cagaacgtaa tttggcatta tactggtagc ccattgaaaa tccgtgggca
    11701 cctgtatcat gaattaccag caaatcaccg atttctgtat gaggcagttc gcgattgact
    11761 gcaaatttat cattgttttc acagagtgaa ccaaccacat ctaccgcttc agctggtcca
    11821 tctggatggg tcacgttgct aatatgatgg taagctccgt acatagctgg acgcatgagg
    11881 ttgactgctg aggcatccac acctagatag gtacggtagg tttccttctt atgagtgact
    11941 cttgtgacta gagcaccgtg aggtgccagc ataaaacgac ccaattcggt gaaaatcttg
    12001 acctgaccaa gacctgctga cgtaagaact tcttcataca ccttacgaac tccctcacca
    12061 atcaaggcga tatcgttcgg ctcctggtct ggacgataat taacaccaat accgccagaa
    12121 agattgataa agtctagcca aatgcccaac ttttccttga tttcaacagc cagttcaaag
    12181 agctgacgag ccaactctgg ataatagaga tgggtcacgg tattggacgc taggaaggag
    12241 tgaagcccaa aagtcttggc tcctttttcc ttcaagatag caaaggcttc aaagagctgg
    12301 tccttggtca tgccaaactt agcctcccca ggattgtcca taatgtctgt ccccagttca
    12361 aaaacgcctc caggattata acgacaagag atgatttctg gaatgcttgc tactctctcc
    12421 agatgttcaa tatcttcaaa ggcatccaag ttaatggtcg cacccaattc acgtgcatag
    12481 gcgtattcct tgtctggcgt gttgttggaa gagaacataa tctcagaacc cagaaagtcc
    12541 agtttatggc tcatcaaaag ctctacataa ctagagcagt ccacaccaca accttcttct
    12601 tggagaattt tcaaaatagc tggagttgga gtagccttaa ctgcaaaata ttccttaaag
    12661 cccttgttcc acgaaaaagc ttggttgaca gctcttgcct tctcacgaat ccccttctca
    12721 tcatacaagt gaaagggagt cgggaactcg gcaacaatcg cttctaactc ttctctattg
    12781 ataaatggtg ttttcataag cttccttcta ttctgtttgc aaagaaaggc tgggacaatc
    12841 gttccaacct ttagttctat ctcttattta tcttcgtcaa agatattgcc aacctcaaca
    12901 tcgatggctt ggttcttgac atcaatatta gttaccttca agagtgactt gtagtcaaac
    12961 tgcttttcac gttcttcttg ggcattgtcc gcaaagaccg ctacacctgc cagttctgag
    13021 tcgaactcgc gcaagagact aatcataccg ttgaccgttc cgccaccttt caagaagtca
    13081 tccacaatca agacacggct gcctgcctta agactacgtt ttgaaaggaa cattttctcg
    13141 atacggtcac cacttgaacc tgaaacatag ttgacgctaa cagttgaacc ttcggtaatt
    13201 ttcaggtcac ggcgcacaat gacaaaagag acattgagga cattggcaac tgcatttgca
    13261 agtggcacac ccttagttgc tacggtcata accgcgtcaa ttttttggtc cataaagctt
    13321 ttggcaataa tacgaccaat atttttcaag atggctggtg tgctaagcaa atcagacaga
    13381 tagatataac cacctggcaa gatacggtca ctttctgaca acttggtacg caagtcttca
    13441 accatttcct tagcatcctg actcgaaata gacggtgtga agatgacacc tccgccagcc
    13501 ccagtcactg tctggatatg accgatttca atttcctcaa aggcgcgttt gataatgacg
    13561 atatcttctg agatggatga tttagcagac tcatactttt cagcaaaagt attgagacta
    13621 gttagtttat aaggattatt aatcaaatag ttggaaatga caaccatccg atcacttctt
    13681 cttaatttca tttctatttc ctattccatt tttattcaaa aaatcaaaaa gcaaactaga
    13741 aagctggtcg ctggtggttc aaaacactgt tttgagattg tcaatagaac tgacaaaccc
    13801 tgtaatatac ctgcatatat acatacgaca aggcgatact accctagttt gaagagattt
    13861 tcgaagagta ttcatttttg tcttttactt attataccat attcacataa aaaaacgaac
    13921 attcttatcc taaaaaatgc tcatttttct taaattatta atctaaatct ggtttataga
    13981 aggaacgatt atccatagcg aagattttat tggacatctc tcctttatcc accaaagcca
    14041 gagctgttga catcatcatc atgcttgcat ccagattgtc aatcatatgg ataatctctg
    14101 cctccataat acgtggacgg actggacttc catattcaag caagccgtgg tgactgagga
    14161 tgacatgacg aagcaaaacg acttcttcct tggtatcatc gatgccgagt tccataactg
    14221 tcttggtaat ttcgctatca atcagagcga tatgtccaag aagattgcct ctgaccgtgt
    14281 actcggtctg gtctggtccc gtcaactcga tgaccttagc caagtcgtgc aacataatcc
    14341 ccgcatagag caggctctta ttgagctgag gataaacttc gctaatagcg tctgccaaac
    14401 gcaccatggt cgccgtatga taggccaagc ccgtttcaaa ggcatggtgg ttggtcttgg
    14461 cagctggata ggagtagaat tccttatcat acttggtgta gagatttcgg acaatccgtt
    14521 gccagacagg attttcaatt ttgaaaatca tttgcgacat gtagtcacga atttccttga
    14581 catcaactgg tgacttgacc ttgaaatcag ctgggtcatt gggttcacca gcttgaggca
    14641 ggcggagagt aatttgattg acttgagggg tattgttata aacttctcgg cgtcctttca
    14701 tgtggacaac cttacctgcg gtaaaggcct caatgttatg aggttgggca tcccagagct
    14761 tcccatcaat ctcgccacta tcatcttgga aggtaaaggc taggtagttt ttcccagctc
    14821 gagtttgcct caggtcagct gatttgatta ggtaaaagcc ttcaaatagc tcatcttttt
    14881 tcatgtgact aatcttcata ttcttcctca ttttcttgaa aatggagtag atcaagcgca
    14941 ggctcgcctt ctgacaactc aatgtgacgg agcgtccgct cgatagctat ggtacgacgg
    15001 tttaataatt catcaatatt gccagaggca tgttggagat gtttttgtgc cttgaccaga
    15061 ataccaccaa acttgccaaa ctcggtcttg acactggcaa gagtcttgct gatatggtcg
    15121 gcactctttt ggatattaag ggtcttgaaa ccaactgata gggagttaag aagggctgat
    15181 agggtacttg gtcctgcaac gataatctgt tcttcccgtc tcaaatcatc aaagaagacc
    15241 ggattgcgca caatctctga ataaagacct tctgtcggaa caaacaagat tccaaaattg
    15301 gttgtccgag gcggtgctat gtacttgctc ttgatatcct tggcaaagcg cttgacgctt
    15361 gctaggagtg acttacgaca gcgttcaatc tcatccttgt cacctgtctc ataggcttct
    15421 tccaagcggt aataatctgc cagtggaaac ttagagtcaa ttggcagata gacgtattct
    15481 tggtcgcctt gtccgggtaa cttgatggca tactccactc gttcactaga gttttcaacc
    15541 gttgcgtatt ctcgttcgta ctgggcaggt gtcatgatgt cttcaataat ttgccccagt
    15601 tgcaattctc ccagaatccc tcgcgtcttg gttccagaga gaaccttgtt aagagctccg
    15661 acatcacggg caactgtctg catttctcca agaccacgat tgacagactc cagttgttta
    15721 gaaactgtct caaaggaagc ctgtaagcgt gtctgcaagg tcttttctag tttttcctcg
    15781 accgtctggc gcatttgttc caaacgttgc tcatttgatt cctgcaaggc ttggagacgt
    15841 tggtctgtct tgtctctagt ttggaggaga ttatctgtca tttcttgacg gacttgggtc
    15901 aggccttggt gcaattcaat ccgcacttct tgcaaacggt cgctgacagc cacttccaaa
    15961 tctttttggt ctaactggct ggcttgtctg gcttggtcaa agcggtaatc caactggtct
    16021 gacaaatgat ctgcctgatc ctccaagctc ttacttaagt gtttctcctg cctatcctgc
    16081 ctttgccaaa tcagaaagag accagctagg ttggcaatta ataatagaat aagtaaactt
    16141 tccatcctac ctcctgtcct tgctatgcag tacgaccaca taaccatctg ggcaagttac
    16201 cgacacttcc ctatctatat attcgttaga agcgtacact tttttaaaga aaaaattctc
    16261 ctctgtcaac tcatacttag ctccaagaat agtcagctgg ctatcccgaa ctggcataaa
    16321 ggctagatag tcgtagtctg aacgaggttc tagctgactg gtcccttctg aacaataggc
    16381 aatcaaattt tgcccatcct caatttctat ttgacgcata tagggtgcca acttaggatt
    16441 gctaggtaga aagacattgg ccaacatatg gtcaatacgg ccacccaagg caccgaaaat
    16501 agtgacctga gcctgaggat tttgttcaaa gatggttaag agagccaatt ccagatctgt
    16561 atcatccttt tctggtcgtg cttggacaaa atactgggca cgtttttgaa tcacctgtcg
    16621 ctcttcttcc gtcacagaat caaaatctcc gactgctaga gcaagaggta agtcttcttc
    16681 caagacccag agcgagcctc gatccacccc aacaaaagca tcaaaatctg tccgataatg
    16741 accgcggttt ccgcctgcaa aaactgcaac cctagtccag ttgttttctg agagtttgta
    16801 ctcgctcatt gacttctccc ttaaagacat aggaacctgc tacaaaaaca gtcgcaccag
    16861 cttctttggc ttgagcaata gtttggtcat caatcccacc atccacttcg atttcaaagt
    16921 tcaaaccttt ttcctcacga agagcaacca actcacgaac cttatccatg gtttctggca
    16981 gaaaggcttg cccaccaaaa cctggattaa ccgtcatgac taaaacttgg tcaactagat
    17041 gaaggacgtg cttgatggct tcaactggtg tgccaggatt gataacgact gaaggcttaa
    17101 ctccgagtga acgaattttt tggagggcgc catgaatatg aggtgttgct tctacatgga
    17161 tactgatgat gtctgcacct gcacgcgcaa aatcttccag atgatgctca gggtttgaca
    17221 ccatcaagtg gcaatcgaaa accatcttac tatgaggacg aaggctctcg accacacctg
    17281 caccaaaact gatttgcggt acaaaatgac tgtccatgat atcgatatgg gcatattctg
    17341 ccccagttgc ttctagacgt ttgatttcac gttcaaagtt ggcataatct gctgccagaa
    17401 ttgacggagc aatcttgtat tgagacatag gtttctcctt attttggaat ttttttgctg
    17461 acttttttat aggtttctct acgattttca atttcactaa ggaattgcag gtaattgtca
    17521 aaacggaagg ttgcaataac accctcttca acagctggtt tgacggcaca agacggctca
    17581 tgggtatggg tacaagtacg gaacttacaa tctcggctaa cagtagcaat ctctgggaaa
    17641 gcctgattga ggtcttcagc ccttgatact tcatagtcca aggatgaaaa tcctggtgta
    17701 tctgcgattt tacccccgtt gagattgtaa aaactaacag ctcgagtggt atggcgaccg
    17761 cgacctagac tgtctgaaat ttctcccgtt tcaagattga ggtctggtgc gattttattg
    17821 agaagagttg acttcccaac acctgtctgc cccataaaga ccgtaacctt gcctgttaac
    17881 aaagacagga gttcctcttt actggtcaca aagtcatagc cgatgtcacc ataggtctgc
    17941 tggtaaaaat ccagttctcc cctatcttcc aacaaatcca ttttggaaat atagacaatg
    18001 ggatggatgc ccttgtgctc caaaagaacc aagaaacgat ccagcaaatt gctgttaaaa
    18061 tcaggttcct tgacggacat gattactaca gcttgatcga tattgacaat aggcggacga
    18121 accagactgt ttttccgttc gtgaattttg aggatatagc cttctgaatt ttcctcggca
    18181 gagaaatcta cccagtcccc aacataaggg atatggcctt ttttacggaa attcccacgc
    18241 gcgcgtgttt gataaacctg gccatcactc tccacatagt agaaacctgc caaggcttta
    18301 atgatttgtc cctgcatctt agagtccttg ccctttaagt gcgtctgcta gaccggcaaa
    18361 ttctgccaag ctgagagctt ccccacgcac acttggtgac aagcctgcct ggtccaaagc
    18421 cttggtcagc ttgtccttga cctcttcagt cttaccaaag taacctgtca agttattcca
    18481 caaggtcttg cggcgatggg taaaactagc cttggaaacc ttaaagaaaa agttctcatc
    18541 ttctactgct acggctggct ctggacgacg caccattttc aagatggctg aatccacatt
    18601 tggcgctggc acaaagaccg tacgaggcac gataaaggca accttggctg tcatgtaata
    18661 ctgcacggcg atagacaagc taccgtaagc cttggtgtta ggctgggctg aaatgcggtc
    18721 cgctacttct ttctgcatca tgaccacaaa ctcactaaaa ggaatgccac tctcaatcaa
    18781 gtgcatgaga ataggcgtcg tgatgtagta aggcaaatta gccactacct tgattggcag
    18841 gttaggattt ttaaaatttt ggatatgttg cgccaaatca accttgagaa tatcttcgtt
    18901 aactacggtc acattatcaa aatcacgcag ggtatctgcc aaaattggca ccaaacggtg
    18961 gtcaatctca aaagccatga cttgggctgc acgctcagcc aaaaattctg tcaaggcacc
    19021 aatacctggc ccgatttcga tgacattgac ctgatcatca atttcagccg tatccacaat
    19081 tttttgaagg atattggtat ccgtcaggaa attttgcccg aaggactttt taaaggtaaa
    19141 accgtgacgc tccagcactg ccttggtcac gctataatct gcaattctca tctattctct
    19201 tttctattat ctgttactac tattgtaaca cattcccctt gtattttggg aatgaaaatc
    19261 ataccaaaaa actatctttc ctcatgaaga aagatagtct tggaaatctt taaaagtggc
    19321 cttagcagtt ctctcgaaac atgcgttcaa aaaaatcagc ttcttgataa cttcgatttt
    19381 cgggtagggt caaatccaca tcctcctcca catcatccac tgtcagttca accgtctgag
    19441 gtaaattttt caaacgataa tgcttttgcc tttgcccttc aatactagca aaatcaagca
    19501 aatagccatg cactcggatt tgaaccaaaa ggagatgatt ggtcatattt tccatgatga
    19561 ggatatcttc taaaccacct ctggcactag gataatcaga taccctgcga atacggattt
    19621 tttcacacat gagcaagttt cctcatatca atgatttcat cagccttatt ccagactagg
    19681 ggattatggg tcgcaatgat gataattcga ttttcatctt tcaaatccac caagagattc
    19741 ataacctcct ctgaattttc aggatcaaga gctgctgttg gttcatctgc caaaatcaag
    19801 ggtggatttt tcaaaatagt cttagcaagg gcaactcgtt gggcctctcc cccagataaa
    19861 gtatagattt tttgttctaa atccaaatac cctagattaa ctttttctaa agcccccact
    19921 tgcctttcca aacgttctac ttttgagatt ttctgaccaa caaaacccaa atccaaattt
    19981 tctttgattg attggttttc taagaggccg aaattttgaa agagatagcc catctggtct
    20041 cgaaaatact cacgagtggg aatggttttt aaatctttcc cctgatagag aacccttcca
    20101 ccatctatct tttctagctt tcccaagata ttcagcagcg tcgtctttcc acttccactc
    20161 tttccgatta aggcataaac cttgcccttc tccagcttga gattcaaacc agagaaaatc
    20221 gcgcggtcat taaatttctt ttccaatcct tgaatatcaa tcatcttatt ttcctttcaa
    20281 tactgccatg ctaccagctt cttctttttt gtcctgcctt actaaaatca agagggcgtt
    20341 aagcgtaaac aaagctacaa ctagagcgct aatcaaacca tctcttgtca aaatactaga
    20401 taggactagg ccaagcaaga gaactcctcc ttgcgccagt aaatacttgc catgaagctc
    20461 atagattgtc ataccagtaa gacgtttaat cataagatcc cgtctgaact gctcaaaata
    20521 gagaagattc atggaatcaa ataacaagat agccgtagac agggtcaaaa tcgtcccaat
    20581 aaggagagag taaaactcca cccgtttgtc atttagtacc ttggcaaaaa atagctggct
    20641 ttttaccaag taagaaacct tgtgatacag accttgctct tttagagctg tgatgctgtc
    20701 tccatatcga tctagtttca aggcactatt agccgtagtt ccccacaaca tccctgcaac
    20761 aggtcttgct ccagtcgctt gcggcgttag aactacaata atcggatctt ttaggtactg
    20821 gcgtgttgtc ttgtacccat cattatagag gaaacgttcc tgtcctgttt ctgtaaaagc
    20881 tagccttacc tgtgggaggt agtgtttctg actcgtcact tctacacttt cagatgaaaa
    20941 gttttgcagg taatctgtaa acaatccttg gtagtagaca gactgctctc gcaagctctc
    21001 aggcaagatc agcccaaact ctccctcaga caagttttgc atcttgtcca taaactctga
    21061 agaaacggta atcttttctt ctatcagata gcgtggtgag acatagataa cattccctga
    21121 cggtgtgtag tcactgagac gattgccatc taggtccact tctgctccat ctgagaaatt
    21181 gtcaacattg ctcataatat aaaaagagtc tgtattggct aaccgttctt cagtaaatgt
    21241 ctgccactca cgattatcct tacgcgttcc ttcttcatcg gcaaatgcac tagaccaacc
    21301 aaaggataga cggtaacggt ctgaggactg gctccattta ttgctagctc tctccatttc
    21361 ctgcatttca cggtagtggg gtaggagagc tgtcgcactc gatccgacca ccaatacagc
    21421 taagagttgc cccaccatca tcaatgtcat catacgtttg agagggagtt tccctttcaa
    21481 tagatccacc agactatttt cctgtaaacc aagtagatag acgacactta gtaaggtaga
    21541 aatccctgcc aaggtcaatc cataaagtag aagagcaatg atgaccagtt gcaccgttgc
    21601 cataaacaag gcaccttgat accagagaat ccccaatccc aaaagactgg ataccagcac
    21661 tgagcagata agctgtctca catcttctaa cactggtctg agagcaactc caaacaagct
    21721 ctcaccagct attaagcgaa tccctgcctg acgaagggat ttgatccgat aaatcagggt
    21781 cagactcata aaggtcagca gaaaaatagc taaactcagt agcaccatag gggtggccgt
    21841 caatagtaag actatcgaaa atggatcttc tccattcgaa acaaagcctt gataaccaag
    21901 ctctttcaag gtggtctgta agctcactcc atccaaactt cctgacacaa tcaagtaaga
    21961 ccctaataaa tcactcgttt ctgcactctc cttggaggaa atggtcagac cttctggaag
    22021 ctttccctca ccataagtgg cataggtaaa gcgtgtttct ccagcttcat ttggctcaac
    22081 gattcgacgg gcgatgagac tattatgctc taccgccaag tgactcaaag cctctgtcac
    22141 ctcttcgcga ctagctactg gttgatttgt catcattcct gtcacatcaa cagaaggata
    22201 gttaccaaat aaaatctctt ctcgatggtc aacggaagtt atcaccatat aaagcgatac
    22261 caataccatt gaaatcaaaa taaataaacg tttcatcatt tcctccaaaa agttacttgg
    22321 tcacttagtg agaaggtgac cacagtcact gcgatgttaa gaagcttatt aagttctttc
    22381 tgctagcaac atcttcaaat acattatatc cgttttttca agaactatca aggacatcaa
    22441 tgtccttaaa atctaactat ttcatattat ttttagctta tatctaaaaa aacagttcta
    22501 aagacgaact ttagaactgt tttgatacga ggacaaggac atttttgtca ctgattcact
    22561 atgtttataa atattttttg acatcctctt gccattcctc tgtcaatttc tttactagaa
    22621 atccatctcc aattagcttc gcaaatgttt tagaagactg ataaaatgat tcagctaaac
    22681 taaaatctct ttctactctt aatgcttgct tccacaaaaa cataaataca agaggtttct
    22741 tttgataatc ttgagtttta tccataattt cttgtaaaca cgatagataa tcattaaaac
    22801 aatcagttac tcctaccttt tcaagacaac acattcctga aataagagta tctctaatta
    22861 taaagtaatt ctctatttga gttaccttct tctgttccaa aagaaagagc attagctttt
    22921 cgatttgatt tataaatttg gcaagatctt ttctaatgag catttgataa aaaaacaaac
    22981 gaatcaacaa catatcattt attgaataag cttctttttc tataatctga ggcatatgtt
    23041 cctcaagtat taactctgca tattcaggcc ttctagttct agaagtatcc aaacacgcct
    23101 gcaatacttc acatgctaat tgttcttctt taggaagatt atcaaagtat gttttataaa
    23161 tctcttccaa acacgcttcc ttcttatcgt actcctcttc tttaccatag attggttcac
    23221 gtaaaatctg gtatttcaat tctaaataag cagaaggaag agctgaaaaa tccggcataa
    23281 ggctataaac tggaactcct agccggcgag caatataggc taacttgggc aaactcggtt
    23341 gcgaagctcc actttcaatt cgcgacagtt gacgaactgt cagtccttgc tcatctccac
    23401 aaaaatctgg acgactaatt cctttttcta tccgaatctc tttaattttt tgaccaatta
    23461 acatcagacg cctcctcttc tacctgcttc gaaaaatact gtgaaactta aaaaattgta
    23521 acggtcacat ctttcctact cataagattt catagcttct tccacttctg ccaaagtaac
    23581 cccaaacaac tctaggcgtt tgaggagttg cttgccgtta gaatagccga ttcggagagt
    23641 ctctccgaga tattctctac gcttacggct gtctgcccct gctagaaaac caaggcgaat
    23701 caaatcgcta cgactaatgt caaactgact ctcatgttca aattgttctg tcacctgagc
    23761 tagagccgtt ttcaggtctt catagctggc atgctcaatt cccagagaac gccccttggt
    23821 cttggacttg ggaacagctt catctcgctt gagaaaggca tgctgaactg ttggaatggc
    23881 cgtcataatc atgcgccgaa tccgctcccc attaaaatct gggtctgtaa agacaatgac
    23941 tccatgacgt tggtgtagac gctgaatccg ctctatgtcc tggtcattga tggcagaacc
    24001 tcgagtctca taggtctcca catcgaaata acgcttgaga ttgaccgtat catcacgacc
    24061 ttcaaccacg ataacttggg aaattctctc tttcattact tgctgtccaa tccaaaaatg
    24121 cgttctgcat ttgcagtcgt tgctaccgcc agctcttctg tcgtcatacc acgcaagtca
    24181 gcgataaagt cgaccacata gcgagtatag gctgttttat tttcacgacc acgcttgggt
    24241 acaggtgcta agtaaggcgc atctgtttct accaacatct tgtccaaagg taactcttta
    24301 gctgcttctt ggaggtcagt tgccttcttg aaggtcacca ctcctgagaa ggaaatggtc
    24361 ataccaagat ccacaaactt ctctgcccac tcaagcgtcc ctgaaaatga atgcatgata
    24421 ccaccacgag gaccaacgcc ctcactcttg ataatctcat aggtatcttc cagcgcatca
    24481 cgggtatgga caacaaaagg caaatccaag tccttagata gctgaatctg acggcgaaaa
    24541 acctgctcct gcacctcttt gggcgctgtc atccaatggt agtctaagcc aatttcacct
    24601 aaagccacaa ccttggaatg ttttaactta tccaacaagt aagcctcaac ttcctctgta
    24661 taagtaccag cttctgtagg atgccaacca atagtcgcat agagctgctc atactcatct
    24721 accaactcca aggcatgctc aatcgtcggt ttatcaaaac caacaatatt catctgtgtc
    24781 acacccatct cagcagccaa ggcaatttct tctgcctcac gacctgcaaa ttcttctaca
    24841 ttcaagtgtg tatgtgtatc aaaaatcatc tcttctaacc tcgttttcta tccttctatt
    24901 ataccaaaaa agaggagggg cacctaattt ttcggtttcc cctcctctct tcaatagaga
    24961 gctattctgc tatcttttct atccgatatt gcccatctcc tattccacag ttagagacag
    25021 aagagattgg ctacattgat agaatattcc tattcagcta aagtatgtga ttatcttggg
    25081 ttctcacttt tctcacccat cactaggatt gatgacttct caaaatcccc cttataatct
    25141 aactttaagt agagaataga attttctagc tgttctattt tctacgtcta gtctttcttc
    25201 ctagcaaagc aaaaccactt gttgccacca ctagaccaag actagcaagt gctgcacttg
    25261 atgctgaacc tgtgttaggt aattgtttgc cagcaggtga ctggtaggta acatcttgca
    25321 ttgttcctgc agttgcttgc ggtgcacttg ttgtcttagg agcagcatta ggagccactg
    25381 gtctcttagg aactgtaacc atggcaatgg cttgcactcc aatcgtttct gcttccttca
    25441 catcttccat agttttcgca ttttcaatct ctttgagagc tgcttgtttt tctgcttcca
    25501 ctcttgctaa aagttctgct ttttctttat cagaaagcgg tgcgcctttg atttcatcct
    25561 gcttgttctt ggctgctttt tcaatggcat ttttagctga ttctttttca gtagctaatt
    25621 gcttagaagc ttgtgattga gctgagtttt gtgaacctgt gtgagctgat ccacccgcct
    25681 ggctaccacc gccttcattc gcattagcat ctgagcttcc agtattgcct cccttatctc
    25741 cacctggtgt attttctagc ttatactctg gtgtagcttc ttgagttaca gattcacctt
    25801 tcgcagattg ttgaactgta tcttttccta gaatcgtcac tactgaacca tctgggaatg
    25861 tgattgtcgc tgtgccatct ccagctactt caactttata gtaaaataaa ataataacag
    25921 gacaaatcga tcaggacagt caaatcgatt tctaacaatg ttttagaaac agaggtgtac
    25981 tattctagtt tcaatctact ataacatcgc ttccgaatcc atttgcatct actgaacttt
    26041 tttacaaaaa taaaaatcag tagactcgag ttcctactga tttttgatag aattcgtttg
    26101 gattatgcag ccaaaacttt gcgtccttta cgacgacgag ctgccaatac gcgacgacca
    26161 tttttagttg acatacggtt acggaatccg tgtttgcgcg cacgacgaag tttacttggt
    26221 tgataagtac gtttcacgat gaatacctcc tcatagattt tgtattcgtt tagccggcta
    26281 taaagtgatc agttactaaa catactttac tattctatct gattctttca cttttgtcaa
    26341 tagccctagg caaatgtttc cagctttttc gaatgcaagc cct
//
LOCUS       DAVLVZ010000024        23113 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000024 DAVLVZ010000000
VERSION     DAVLVZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.024"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            329..676
                     /gene="ssrA"
                     /locus_tag="VYN77_001018"
     tmRNA           329..676
                     /gene="ssrA"
                     /locus_tag="VYN77_001018"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(859..946)
                     /locus_tag="VYN77_001019"
     tRNA            complement(859..946)
                     /locus_tag="VYN77_001019"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(910..912),aa:Ser,seq:gga)
     gene            1109..1849
                     /locus_tag="VYN77_001020"
     CDS             1109..1849
                     /locus_tag="VYN77_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1558494.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1846..2757
                     /gene="pfkB"
                     /locus_tag="VYN77_001021"
     CDS             1846..2757
                     /gene="pfkB"
                     /locus_tag="VYN77_001021"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1558495.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2754..4706
                     /locus_tag="VYN77_001022"
     CDS             2754..4706
                     /locus_tag="VYN77_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1558496.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALTELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSND
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4870..7095
                     /locus_tag="VYN77_001023"
     CDS             4870..7095
                     /locus_tag="VYN77_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1558497.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7294..7719)
                     /locus_tag="VYN77_001024"
     CDS             complement(7294..7719)
                     /locus_tag="VYN77_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558498.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            7857..8999
                     /locus_tag="VYN77_001025"
     CDS             7857..8999
                     /locus_tag="VYN77_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1558499.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            9008..10222
                     /gene="thiI"
                     /locus_tag="VYN77_001026"
     CDS             9008..10222
                     /gene="thiI"
                     /locus_tag="VYN77_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1558500.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEVTPQAE
                     KDEVDDLIDNLL"
     gene            10357..11181
                     /locus_tag="VYN77_001027"
     CDS             10357..11181
                     /locus_tag="VYN77_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1558501.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFYSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYTAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            11203..11943
                     /locus_tag="VYN77_001028"
     CDS             11203..11943
                     /locus_tag="VYN77_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1558502.1"
                     /translation="MHIEHLSHWSGHLNREMYLNRYGHGGIPVVVFASSGGSHNEYYD
                     FGMIDACASFIEEGLVQFFTLSSLDSESWLVTWKNAHDQAEMHRAYERYVIEEAIPFI
                     KHKTGWFDGMMTTGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFVGDYYNDDAI
                     YQNSPVDYIWNQNDGWFIDRYRQAEIVLCTGLGAWEQDGLPSFYKIKEAFDQKQIPAW
                     FAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            11956..13122
                     /locus_tag="VYN77_001029"
     CDS             11956..13122
                     /locus_tag="VYN77_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1558503.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMVPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYIAIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASEFEPQYCLATSRRANAHYVY
                     SEENLLAKYSQQFKVKKIMPEAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            13630..15123
                     /locus_tag="VYN77_001030"
     CDS             13630..15123
                     /locus_tag="VYN77_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1558504.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            15136..16245
                     /locus_tag="VYN77_001031"
     CDS             15136..16245
                     /locus_tag="VYN77_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1558505.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKYL
                     RINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLA
                     LQNEFADFVALVDKSQFACEIAIKVWRNSLKFSII"
     gene            16275..19625
                     /locus_tag="VYN77_001032"
                     /pseudo
     CDS             16275..19625
                     /locus_tag="VYN77_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            19896..19973
                     /locus_tag="VYN77_001033"
                     /pseudo
     CDS             19896..19973
                     /locus_tag="VYN77_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20212..20682)
                     /locus_tag="VYN77_001034"
     CDS             complement(20212..20682)
                     /locus_tag="VYN77_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1558506.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(20699..22984)
                     /locus_tag="VYN77_001035"
     CDS             complement(20699..22984)
                     /locus_tag="VYN77_001035"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1558507.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKASDKALYKMEVELEVKLPHKIELEEPQLNLVQPQGKAELIAEAEEKLTHINSSYT
                     LNDYFLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQ
                     RQVKADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVT
                     SPGGYPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHD
                     RNYLLNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNW
                     QSIDFRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLG
                     QEEQAIQNQYPDKDFERYSKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLR
                     IKSSTNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRV
                     VTKGYLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITI
                     RDNTDYHLTVDLAQSMLTLPC"
BASE COUNT         7012 a         4141 c         5047 g         6913 t
ORIGIN      
        1 caataagtga aaagtagtcc gaataatata aggattgatt ttatagtttt taaactcaaa
       61 tgaattaaaa taaagagagt acgaaaattc tcatctgaaa gtattttaga ataattctct
      121 tcgtgaattt cttcaaaaca gatagcttca tcttaggtat gtgttaattg gatttcgtaa
      181 gctctattta caatcgtaaa gttatgcact gaaaaagttg tagttagtct tccagtttgc
      241 taaatttctc gaatagtatc ttttttaggt atgtgatttc tttttgcatt tttgagttag
      301 ataaggtata atggttttat tgtcttttgg ggtcgttacg gattcgacag gcattatgag
      361 gcatattttg cgactcgtgt ggcgacgtaa acgctcagtt aaatataact gcaaaaaata
      421 acacttctta cgctctagct gcctaaaaac cagcaggcgt gacccgattt ggattgctcg
      481 tgttcaatga caggtcttat tattagcgag atacgattaa gccttgtcta gcggcttgat
      541 aagagattga tagactcgca gtttctagac ttgagttatg tgtcgagggg ctgttaaaat
      601 aatacataac ctatggttgt agacaaatat gttggcaggt gtttggacgt gggttcgact
      661 cccaccggct ccattattcc tttgcattct tttgcattcc ttggtaaaac gttgttaaat
      721 caacgttttt tatttttatc tttggtattc ctttgcattc ttttgctaaa aagggagtca
      781 caaacagacc ctattttaaa aaaaggatag aaaaaaggat acaacatttg tcgcatccta
      841 aaaataatct tttttcgacg gaagacatgg gattcgaacc cacgcacgct attacacgcc
      901 taccgcgttt ccaacacggc ctcttaagcc tcttgagtaa tcttccaata cttactcaaa
      961 tagtctacca taaaggctct tatcttgcaa taaaaattct agaaataaga aaaaatgata
     1021 gattttgaaa gaaaatgata aaaaatgctt gacttcgaaa gaaagtatga tagaatgaat
     1081 agtgtaaacg ataacaggag gtgattcagt gttaaaaaca gaacgtaaac aactaatttt
     1141 agaggagtta aatcaacatc atgtagtttc tctagaaaaa ttagttagtt tgctagaaac
     1201 gtcagaatca acggttcgaa gagacttgga tgagttggaa gcggaaaaca agcttcgtcg
     1261 tgtgcatggt ggagcagaac tcccccactc cttacaggaa gaagaaacca ttcaagaaaa
     1321 atctgtcaaa aaccttcaag aaaagaagtt gctggctcag aaagcagctt ctctcattaa
     1381 agaaaaagat gtcatcttta tcgatgctgg aacaacaact gcttttttga ttcatgaatt
     1441 ggtcaataag aatgttacag ttgtgaccaa ctccattcac catgccgctc agttggttga
     1501 aaagcagatt ccaactgtca tggttggagg aaacgtcaag acggcgacag atgctagtat
     1561 cgggggcgtt gctcttaacc agattaacca attgcacttt gaccgtgcct ttatcggaat
     1621 gaatggtgtt gacgatggct attatacgac tcctgatatg gaggagggag ctgtgaaaag
     1681 agctattttg gagaatgcca agcagaccta cgtcttggtg gattcgtcaa aaattggaca
     1741 aacttgcttt gccaaggtag caccactcaa acgcgctatc gttatcacta gtcaagggca
     1801 tgagctcttg caggttatta aggagaaaac ggaggtaata gaagtatgat ttatacagtc
     1861 acactcaatc catccattga ctatatcgtt cgtttggacc aagtcaaagt tggtagtgta
     1921 aatcgtatgg acagtgatga taagtttgct ggtgggaaag gaatcaatgt cagccgtgtc
     1981 ttgaaacgtt tgaatatacc aaatacagcg acgggattta tcggtggctt tactggtaaa
     2041 tttatcacag atactttagc agaggaagaa atcgagacac gttttgtcca ggtggcagaa
     2101 gatactcgta tcaatgttaa aatcaaagca gaccaagaaa cagaaatcaa cggaacgggt
     2161 ccaactgttg aatcggttca gctagaagaa ttgaaagcta ttttatctag tctgacagca
     2221 gaagatacag ttgtctttgc aggttcaagt gctaaaaatc taggcaatgt tatctataag
     2281 gatttgattt ccttgacgcg ccagactggt gcgcaagtgg tctgtgactt tgaaggacag
     2341 accttaattg atagtttgga ctaccagcct cttcttgtaa aaccaaacaa tcatgaactt
     2401 ggagcgattt ttggggttaa actcgaaagt ttagatgaaa ttgagaaata cgctcgtgag
     2461 ttactggcta agggtgctca aaatgttatt atctctatgg ctggtgatgg tgcccttctt
     2521 gtcacatctg agggagctta cttcgctaaa ccaatcaaag gaacagtcaa aaattcagtt
     2581 ggagctggtg attctatggt tgctggattc acaggtgaat ttgtcaaatc aaaagacgta
     2641 gtagaagcct tcaaatgggg agtggcttgc ggaacggcaa ctaccttctc agatgacttg
     2701 gcaacggcgg aatttattaa agaaacatat ggaaaagttg aggtagaaaa acgatgaaaa
     2761 ttcaagacct attgagaaaa gatgtcatgt tgctagattt gcaggcaact gaaaaaacag
     2821 ctgtcatcga cgagatgatt aaaaatttga cagaccacgg ttatgtaaca gattttgaaa
     2881 catttaaaga aggaattttg gcgcgtgaag ctttgacttc tactggtttg ggtgatggaa
     2941 tcgcaatgcc tcacagcaaa aacgctgctg tcaaagaagc gacagttcta tttgctaagt
     3001 caaataaggg tgttgactac gagagcttgg atggacaagc aactgacctc ttcttcatga
     3061 ttgcagctcc agaaggtgcc aatgatactc acttggcagc attgacagaa ttgtctcaat
     3121 acttgatgaa agacggtttt gcagacaaac ttcgtcaagc aacatctgca gaccaagtta
     3181 tcgaactttt tgaccaagct tcagaaaaaa ctgaggaact tgttcaagca cctgctaatg
     3241 actctggtga ctttatcgta gctgttacag cttgtacaac aggtattgcc cacacttaca
     3301 tggcccaaga agcccttcaa aaagtagctg ctgaaatggg ggttggtatc aaggtcgaaa
     3361 ccaacggtgc tagcggtgtt ggaaatcaac taactgcaga agatatccgt aaggctaaag
     3421 ctattatcat tgcagcagac aaggccgttg aaatggatcg atttgatgga aaaccattga
     3481 tcaatcgtcc agttgctgac ggtatccgta agacagaaga gctaattaac ttggctcttt
     3541 caggagatac tgaagtctac cgtgccgcta atggtgcaaa agctgcaaca gcctctaacg
     3601 aaaaacaaag ccttggtggt gccttctaca aacacttgat gagtggtgta tctcaaatgt
     3661 taccattcgt tatcggtggt ggtatcatga ttgcccttgc cttcttgatt gacggtgctt
     3721 tgggtgttcc aaatgaaaac cttggcaatc ttggttctta ccatgagtta gcttctatgt
     3781 tcatgaaaat tggtggagct gcctttggtt tgatgcttcc agtctttgcg ggttatgttg
     3841 cctactctat tgctgaaaaa ccaggtttgg tagcaggttt cgtggctggt gctattgcca
     3901 aagaaggttt tgcctttggt aaaattcctt atgccgcagg tggtgaagca acttcaactc
     3961 ttgcaggtgt ctcatctggt ttcctaggtg cccttgttgg tggatttatc gcaggtgcct
     4021 tggttcttgc catcaagaaa tacgttaaag ttcctcgttc actcgaaggt gctaaatcaa
     4081 tccttctatt gccacttctt ggaacaatct tgacaggatt tgttatgcta gctgtgaata
     4141 tcccaatggc tgcaatcaac actgctatga atgacttcct aggcggtctt ggaggaggtt
     4201 cagctgtcct tcttggtatc gtccttggtg gaatgatggc tgttgacatg ggtggaccag
     4261 ttaataaagc agcttatgtc tttggtacag gtacgcttgc agcaactgtt tcttcaggtg
     4321 gttctgtagc catggcagca gttatggctg gaggaatggt gccaccactt gcaatctttg
     4381 tcgcaactct tcttttcaaa gataaattta ctaaggaaga acgtaactct ggtttgacaa
     4441 acatcatcat gggcttgtca tttatcactg agggagcgat tccatttggt gccgctgacc
     4501 cagctcgtgc gattccaagc ttcatccttg gttcagcagt agcaggtgga ctcgttggtc
     4561 ttactggtat caaactcatg gcgccacacg gaggaatctt cgttatcgcc cttacttcaa
     4621 atgatctcct ttacctcgtt tctgtcttgg taggagcaat cgtaagtggt gtggtttatg
     4681 gttacctacg caaaccacaa gcataaaaaa tagaaaaatg aaaagattgg accgtttggt
     4741 gcagtctttt tctcttcccg aaatgcctgt gaaatatggt ataatagaag aatggcaaac
     4801 aagaatacaa gtacaacaag acggagaccg tctaaagcag aactggaaag aaaagaagcg
     4861 attcaacgaa tgttgatttc gttaggaatt gcgattttat tgattttcgc agccttcaaa
     4921 ttaggggctg caggtataac cctttataat ttaattcgct tgctagtggg tagcctagct
     4981 tatctggcga tattcggcct attaatctat ctcttctttt tcaagtggat acgaaaacag
     5041 gaaggactct tatctggctt tttcaccata tttgctggct tactcttgat ttttgaggcc
     5101 tacttggttt ggaaatatgg tttggacaag tccgttctaa aagggaccat ggctcaggtt
     5161 gtgacagatc tgactggttt tcgaacgact agttttgctg gagggggctt gatcggggtc
     5221 gctctttata ttccaacagc ctttctcttt tcaaatatcg gaacttactt tattggttct
     5281 attttgattt tagtgggttc tctcttagtc agcccttggt ctgtttacga tattgctgaa
     5341 tttttcagta gaggctttgc caaatggtgg gaagggcacg agcgtcgaaa agaggaacgc
     5401 tttgtcaaac aagaagaaaa agctcgccaa aaggctgaga aagaggctag attagaacaa
     5461 gaagagactg aaaaagcctt actcgatttg cctcctgttg atatggaaac gggtgaaatt
     5521 ctgacagagg aagctgttca aaatcttcca cctattccag aagaaaagtg ggtggaacca
     5581 gaaatcatcc tgcctcaagc tgaacttaaa ttccctgaac aggaagatga ctcagatgac
     5641 gaagatgttc aggtcgattt ttcagccaaa gaagcccttg aatacaaact tccaagctta
     5701 caactctttg caccagataa accaaaagat cagtctaaag agaagaaaat tgtcagagaa
     5761 aatatcaaaa tcttagaagc aacctttgct agctttggta ttaaggtaac agttgaacgg
     5821 gccgaaattg ggccatcagt gaccaagtat gaagtcaagc cggctgttgg tgtaagggtc
     5881 aaccgcattt ccaatctatc agatgacctc gctctagcct tggctgccaa ggatgtccgg
     5941 attgaagcgc caatccctgg gaaatcccta atcggaattg aagtgcccaa ctccgatatt
     6001 gccactgtat ctttccgaga actatgggaa caatcgcaaa cgaaagcaga aaatttcttg
     6061 gaaattcctt tagggaaggc tgttaatgga accgcaagag cttttgacct ttctaaaatg
     6121 ccccacttgc tagttgcagg ttcaacgggt tcagggaagt cagtagcagt taacggcatt
     6181 attgctagca ttctcatgaa ggcgagacca gatcaagtta aatttatgat ggtcgatccc
     6241 aagatggttg agttatctgt ttacaatgat attccccacc tcttgattcc agtcgtgacc
     6301 aatccacgca aagccagcaa ggctctgcaa aaggttgtgg atgaaatgga aaaccgttat
     6361 gaactctttg ccaaggtggg agttcggaat attgcaggtt ttaatgccaa ggtagaagag
     6421 ttcaattccc agtctgagta caagcaaatt ccgctaccat tcattgtcgt gattgtggat
     6481 gagttggctg acctcatgat ggtggccagc aaggaagtgg aagatgctat catccgtctt
     6541 gggcagaagg cgcgtgctgc aggtatccac atgattcttg caactcagcg tccatctgtt
     6601 gatgtcatct ctggtttgat taaggccaat gttccatctc gtgtagcatt tgcggtttca
     6661 tcaggaacag actcccgtac gattttggat gaaaatggag cagaaaaact tcttggtcga
     6721 ggagacatgc tctttaaacc gattgatgaa aatcatccag ttcgtctcca aggctccttt
     6781 atctcggatg acgatgttga gcgcattgtg aacttcatca agactcaggc agatgcagac
     6841 tacgatgaga gttttgatcc aggtgaggtt tctgaaaatg aaggagaatt ttcggatgga
     6901 gatgctggtg gtgatccgct ttttgaagaa gctaagtctt tggttatcga aacacagaaa
     6961 gccagtgcat ccatgattca gcgtcgtttg tcagttggat ttaaccgtgc gacccgtctc
     7021 atggaagagc ttgagatggc aggtgtcatt ggtccagctg aaggtaccaa gccaagaaaa
     7081 gtattgcagc aataaaaaat agcttctttc caaggttgga aggagctatt ttactggcta
     7141 ttgattactt ttatactctt cgcaaatctc ttcaaaccac gtcagctttg ccttgccgta
     7201 ggtatatgat actgactctg tcagttctat ccacaacctc aaaacagtgt tttgagcaac
     7261 ctgcggctag cttcctagtt tgctctttga ttttcattga gtattatttt ctgaagttgg
     7321 agtattgggc tgtttttcat tttcagtggc aggtttactt gtagcaggtg tagtagagtc
     7381 ctgagttgct gttttctgat cttctttttt ctcttccttg acgctagatt ttggcgtttc
     7441 ttcttgttgt gttttttctt gagaagtgtt attttcctta tttggagtag tattttcctg
     7501 aggggattct ttttgaattt ctttgacaaa ggttgtcgtc tggcttgtcg taggttcttt
     7561 tttagtattt ttattattat ccaaggcgtt catgatcgtg atacccgctg taatcaaacc
     7621 aaggatacta ccgatagtag taatggtttt ttgaaaaaca gtaaagcctt ttttgatatg
     7681 ttctgttttc ggttttgaag ttctacgata gttagacata cattctctcc tttgattatg
     7741 atttattata ctctgtttct ttaaaaaaat cttaaaaaaa gagaaattgc cctttcttgt
     7801 cgcaagctcc gtttcatgat acaatagagg gagtgatttt agaaagcgtg agaaacatga
     7861 tttactttga taattcagcg acgaccaagc cttatcctga agcccttgaa acctatatgc
     7921 aggtcgcttc aaaaatttta ggaaacccat ctagtctcca tcgtttggga gaccaagcaa
     7981 cacgaatctt agatgcttct cgccaacaga ttgcagattt aatcggtaag aaaagcgatg
     8041 aaatcttctt tacctctggt ggaacagaag gggataactg ggttatcaag ggtgtggcct
     8101 ttgaaaaagc tcagtttggc aagcacatca ttgtttcagc cattgaacat ccagcagtca
     8161 aagagtcagc cctctggttg aaaagtcaag gatttgaagt ggattttgct ccagttgata
     8221 agaaaggctt ggtcgatgtt gaggcgttag caggtttgat acggcctgat acaatcctcg
     8281 tttccatcat ggctgtgaac aatgaaatcg gctctatcca acctattgag gctatttcag
     8341 aattcttggc agacaagccg actatttcct tccacgttga tgcggttcag gcgcttgcca
     8401 aaattccgac tgaaaagtat ctgacagaac gggtggattg cgcgaccttc tcgagtcata
     8461 aatttcatgg tgtccgtggt gttggctttg tctatatcaa atctggcaag aagattacac
     8521 ctcttcttac aggtggtggc caggagcgag attatcgttc gacaactgaa aatgtggcag
     8581 ggattgcagc gacagccaag gctctccgtt tatctatgga aaagctagat atctttagga
     8641 gcaagactgg gcagatgaag gcagtgattc accaagctct tctgaactat ccggatattt
     8701 ttgtcttttc agatgaggaa gactttgcac ctcatattct gacttttgga atcaaaggtg
     8761 ttcgaggtga agtcatcgtt cacgcctttg aagactatga tattttcatc tcaacaacct
     8821 cagcttgttc atctaaggca ggaaaaccag ccggtacctt gattgccatg ggagtggaca
     8881 aagataaggc caagtcagct gtgcgtctta gcctagactt ggaaaatgat atgagtcagg
     8941 tcgagcagtt tttgaccaag ttaaaattga tttacaatca aactagaaaa gtaagatagg
     9001 agcattcatg cagtattcag aaattatgat tcgctacgga gagttgtcaa ccaagggtaa
     9061 aaaccgtatg cgtttcatca ataaacttcg taataatatt tcggacgttt tgtctatcta
     9121 tacccaagtt aaggtaacag cagatcgcga ccgtgcccac gcttacctca atggagctga
     9181 ttacacagca gttgcagaat ctctcaaaca agtttttgga attcaaaact tttctcctgt
     9241 ttataaggtt gaaaaatctg tagaagtttt gaagtcttct gtccaagaga ttatgcggga
     9301 catctacaag gaaggtatga cctttaagat ttctagcaag cgtagcgacc acaactttga
     9361 acttgatagt cgtgaactca accaaacact tggaggggct gtattcgaag ccattccaaa
     9421 tgtgcaagtt caaatgaaaa gtcctgacat caatcttcag gtggagattc gtgaagaagc
     9481 agcctatctt tcttatgaaa ccattcgtgg ggctggtggt ttgccagttg gaacttcagg
     9541 taaagggatg ctcatgttgt caggagggat tgactcacct gtagcaggtt atcttgctct
     9601 taagcgtggg gtggatatcg aggcagttca ctttgctagt ccaccatata ctagtcctgg
     9661 tgccctcaag aaagcgcagg acttgacccg taaattgacc aagtttggcg gaaatatcca
     9721 gtttatcgag gtgcctttca cagagattca agaggaaatc aaagccaaag cgccagaagc
     9781 ttatttgatg actctaactc gtcgctttat gatgcggatt actgaccgta ttcgtgaggt
     9841 acgaaatggt ttggttatca tcaatgggga aagtctaggt caagtagcca gccaaaccct
     9901 tgaaagtatg aaggctatca atgctgttac caacactccc atcattcgtc ctgtggttac
     9961 catggacaag ttggaaatta ttgacatcgc ccaggaaatc gatacctttg acatttcaat
    10021 ccaaccgttt gaagactgtt gtactatttt tgcaccagat cgtccaaaaa caaatcctaa
    10081 aattaagaat gcggagcagt acgaagcgcg tatggatgtt gaaggcttgg ttgagcgagc
    10141 agtggctgga atcatgatta ctgaagtcac acctcaagcc gaaaaagatg aagttgatga
    10201 cttgattgac aatctgctct aattcagaaa atccaaaaga atagcgaaaa tcagtaaaaa
    10261 agttagtttt ttctctaaaa acaggtaaaa aactaacttt tttctatttt tatgatataa
    10321 taatataaaa ttttgaatat agagagtttt ctgacaatga atcaatccta cttttatcta
    10381 aaaatgaaag aacacaaact caaggttcct tatacaggta aggagcgccg tgtacgtatt
    10441 cttcttccta aagattatga gaaagataca gaccgttcct atcctgttgt atactttcat
    10501 gacgggcaaa atgtttttta tagcaaagag tctttcattg gacattcatg gaagattatc
    10561 ccagctatca aacgaaatcc ggatatcagt cgcatgattg tcgttgctat tgacaatgat
    10621 ggtatggggc ggatgaatga gtatacggct tggaagttcc aagaatctcc tatcccaggg
    10681 cagcagtttg gtggtaaggg tgtggagtat gctgagtttg tcatggaggt ggtcaagccg
    10741 tttatcgatg agacctatcg tacaaaagca gactgccagc atacggctat gattggttcc
    10801 tcactaggag gcaatattac ccagtttatc ggtttggaat accaagacca aattggttgc
    10861 ttgggcgttt tttcatctgc aaactggctc caccaagaag cctttaaccg ctatatcgag
    10921 tgccagaaac tatcgcctga ccagcgcatc ttcatctatg taggaacaga agaagcagat
    10981 gatacagaca agaccttgat ggatggcaat atcaaacaag cctatatcga ctcgtcgctt
    11041 tgctattacc atgatttgat agcaggggga gtacatctgg ataatcttgt gctaaaagtt
    11101 cagtctggtg ccatccatag tgaaatccct tggtcagaaa atctaccaga ttgtctgaga
    11161 ttttttgcag aaaaatggta agttaagaaa ggaaaaaacg aaatgcatat tgaacatctt
    11221 agccactgga gtggtcatct taaccgtgaa atgtacctta accgttatgg acatggtggg
    11281 attccagttg tggtctttgc ttcatcaggt ggtagtcaca acgaatacta tgattttggc
    11341 atgattgatg cctgtgcttc ctttatcgag gaaggccttg tccagttctt taccctatct
    11401 agtttggata gtgagagctg gttggttact tggaaaaatg ctcatgacca agcggaaatg
    11461 caccgtgcct acgaacgtta tgtgattgag gaggccattc cttttatcaa gcacaagaca
    11521 ggttggtttg atggcatgat gacgacaggt tgctctatgg gagcctatca tgcactcaat
    11581 ttcttcctcc agcatccaga tgtctttacc aaagtgattg ctctcagtgg tgtttacgac
    11641 gcacgtttct ttgtcggtga ttactacaac gatgatgcta tttaccaaaa ctcgccagta
    11701 gattatattt ggaaccaaaa cgacggctgg tttattgacc gttaccgtca ggcagagatt
    11761 gtgttgtgta cggggcttgg agcctgggaa caagatggtt tgccatcctt ttacaagatc
    11821 aaagaagcct ttgaccagaa acaaattcca gcctggtttg ctgaatgggg acatgatgtc
    11881 gcccacgact gggaatggtg gcgtaaacaa atgccttatt tcctcggtaa tctctattta
    11941 taaaaggagt tacctatgaa ttaccttgtt atttctccct actatccaca aaactttcaa
    12001 cagtttacta tcgaactagc taataaaggc atcacagtct tgggaattgg tcaagagtct
    12061 tacgagcaat tggatgagcc cttgcgcaat agcttgaccg agtattttcg tgttgataat
    12121 cttgagaaca tagatgaagt caaacgtgca gttgcttttc tcttttataa acatggtcca
    12181 attgaccgca tcgagtctca caatgaatac tggcttgagc tagacgcaac actcagagaa
    12241 caattcaatg tttttggtgc caaaccagag gatctcaaaa agacgaaata taagtctgaa
    12301 atgaagaaac ttttcaaaaa agcaggtgtt cctgtggtac ctggagctgt tatcaagacg
    12361 gaagcagatg ttgatcaagc agtgaaagaa atcggtcttc caatgattgc caaacctgat
    12421 aatggagtgg gagcagccgc aacctttaaa cttgagacag aagacgatat caatcacttc
    12481 aagcaagaat gggaccattc aaccctttat ttctttgaaa aatttgtcac ttccagcgaa
    12541 atctgtacct ttgacgggct cgtggacaag gatggaaaga ttgtcttctc aacaaccttt
    12601 gactacgcct atacaccgct tgacctcatg atttataaga tggacaattc ctactatgtg
    12661 ctcaaggata tggttcccaa actacgcaag tatggggaag cgattgttaa ggaatttggt
    12721 atgaaagaac gtttcttcca tatcgagttt ttccgtgaag gggatgatta tatcgccatt
    12781 gagtacaata accgccctgc aggtggtttc accattgatg tttataactt tgctcattcc
    12841 ttggaccttt atcgtggcta tgcagccatt gtcgcaggag aagagttccc agcgtcagag
    12901 tttgaacctc agtattgttt ggctacatca cgtcgtgcaa atgctcacta tgtctattct
    12961 gaggagaact tacttgccaa atatagccag cagttcaagg ttaaaaaaat catgccagag
    13021 gcttttgcgg aacttcaagg agattacctt tatatgctga caactccgag tcgacaagaa
    13081 atggatcaga tgattgctga tttcggacaa cgtcaagaat aagaactatc ggattaagga
    13141 aattaactcc cttaatcctt ttgttttgtc tgataaaaaa taagagcatc ccaacaaggt
    13201 agctatcata aaacttgttc gataactatt tgaagcagga ttaggtggct agaaattgaa
    13261 ttttaacatt tcaattgagt catagtattg tgtttgcgta tccttaaatc agctaaaagg
    13321 atccatgacg acacctatac gatatagttt tcaagatacc aaacaagtct attaatactc
    13381 aatgaaaatc aaagagcaaa ctaggaagct agccgcaggt ttctcaaaac actgttttga
    13441 ggttgtggat agaactgaca gagtcagtat catataccta cggcaaggtg aagctgacgt
    13501 ggtttgaaga gattttcgaa gagtataaaa tattcaggtg acgcatagat atagttaatt
    13561 gaagctttgt ttgaaatctg ataaaataat gatattacta agttttaaaa actaaagaaa
    13621 agggaagata tgattacagg cgaattaaaa aataaaatcg atcagctgtg ggaaattctt
    13681 tggacagaag gaaacgcaaa tcctttaaca aatattgaac agttgactta tctcttattt
    13741 atgaaagatt tggatagtgt cgagcttgga cgtgaaagtg atgctgaatt tctagggatt
    13801 ccttatgagg gagtttttcc aaaagataaa cctgaatacc gttggtcaac ttttaaaaat
    13861 ataggagatg ctcaggaagt ttatcgttta atgactcagg agatttttcc gtttattaaa
    13921 aatctcaagg gggatacaga tgatacagcc ttttcacgat atatgcgaga agctattttt
    13981 caaataaata aacctgctac gcttcaaaag gcaatttcta tcttagatgt ttttccaact
    14041 aggggattag atgtagattt tgataatgac aaacaaagta ttactgatat cggagatatc
    14101 tatgaatatc tgttatcaaa attgtcgacc gcaggtaaaa atggacagtt ccgtacacct
    14161 cgtcacatca tcgatatgat ggttgagttg atgcaaccga ctatcaaaga tatcatctca
    14221 gatcccgcta tgggttctgc tggcttctta gtatctgcta gccgttactt aaagcgtaag
    14281 aaagatgaat gggaaaccaa tacagataat atcaatcatt ttcataatca gatgtttcat
    14341 ggaaatgata cggatacgac tatgttgaga cttggggcga tgaacatgat gctacatgga
    14401 gtagaaaatc cacaaatcag ttaccttgac tcgctgtctc aagataatga agaagccgat
    14461 aaatatactt tggttttagc aaatcctcct tttaagggct cacttgacta caattcaacc
    14521 tctaatgacc ttcttgcaac cgtaaaaacc aaaaaaacag aattactctt tctttctctt
    14581 ttcttgcgaa ctttaaaacc aggtggacga gcagcagtta tcgtacctga tggtgtcctt
    14641 tttggttcgt ctaaagctca taaaggaatt cgtcaggaaa ttgtagagaa tcataagctt
    14701 gatgctgtaa tctcaatgcc tagtggtgtg ttcaagcctt atgctggagt ttcaactgcc
    14761 attctcatct ttacaaaaac tggtaatggt ggtactgaca aagtctggtt ttacgatatg
    14821 aaagcggatg gtttaagttt ggatgataag cgacaaccga ttagcgacaa tgatattcca
    14881 gatattatcg aacgctttca tcatcttgaa aaagaagcag aacgtcagag aacggatcaa
    14941 tctttctttg ttccagttgc tgagataaag gaaaatgatt atgatttgtc tatcaataaa
    15001 tataaagaga ttgagtatga aaaagttgag tatgaaccaa cagaagtcat attaaagaaa
    15061 atcaatgatt tagaaaaaga aattcaagct ggcttggctg aattggaaaa attactcaag
    15121 tagggaggtg gctgtatgaa aaaagtgaag ttgggggaag tcttatctct aaaaaaaggc
    15181 aagaaagcca ctgtacttgc tgaacaaaca actctaagcc aacgttatat tcaaatagat
    15241 gatttaagaa ataataataa tttaaaattc actgaaagtt taaatatgac tgaagcactc
    15301 ccagatgata ttctgatagc atgggatgga gctaatgcag gaacagttgg ttatggatta
    15361 tcgggagctg ttggtagtac aattacggtc ttaaaaaaga atgagcgata caaagaaaaa
    15421 attatatcag attacttggg agtctttttg gaaagtaaat cgcagtattt acgagatcat
    15481 tcaacaggtg caacaattcc tcatttaaac aagaatatat tacttgattt acaattagaa
    15541 ttgctaggta tcgaagaaca agagaacatt atctgtattc ttaatacgat taaaaggctt
    15601 attactaaaa gaaaattgca gttagatgaa ctaaacttgc tcgtcaaatc ccgatttaac
    15661 gagatgtttg gggaaaataa aatatttgaa agcattgata acttatttga tattatagat
    15721 ggtgataggg gcaaaaatta tcctaaatca gatgagttgt ttagtgagga gtactgttta
    15781 tttttaaata caaagaatgt tactaaaaac ggattttcat tcgatacaaa gcaatttatc
    15841 actaaaacaa aggataaatt acttcgaaaa ggcaaacttg agcgttatga tatagtcttg
    15901 acaacaagag gtactgttgg aaatgtagcg tactacgatg aattaataaa atataaatat
    15961 ttacgtataa attcaggtat ggtaatatta cgtcccaaga caccaaatct aaatcagaaa
    16021 tttattatcc atgttttaag gaataataat tatagtcgag tgatatcagg aagtgctcag
    16081 cctcagttac caattacaaa attaaaaaaa atacttctcc ctctcccccc actagccctc
    16141 caaaatgagt tcgcagactt tgtagccctg gtcgacaaat cacaatttgc ttgtgagata
    16201 gctataaaag tgtggagaaa tagcttgaaa tttagtataa tatagctaaa ctatttgttt
    16261 aaagtgagaa aaaaatggga aattttagct ttcttttaaa aaatgacgaa tatgaatctt
    16321 tttcaaaacc ttgcattgaa gctgagaata tgattgctac atcaactgtg gctactgcct
    16381 ttatggcgcg tcgtgcttta gagcaggctg tccattggat atatagtcac gattcatatt
    16441 tagaagctcc ctatcgtgct actctatctt ctttagtatg ggatgatgat tttagggata
    16501 tcgtagattc tgaactccac aagcagatag ttctgttgat tcggtgggga aaccatgctg
    16561 ctcatggtgg tgaaattaag gaacgagaag cgattttagc tttgcatcat ttgtatcagt
    16621 ttgttaattt tatcgattat tgttacagca atgagtttgt ggagcgttat tttgatgaga
    16681 agtgcttacc actttcagca aacatcaaat accgagaaac tccacaatct atgataaagt
    16741 tacaagacag tttaccagaa ctgcctgatt ttcatgaaca gatggctgct cagtccgtag
    16801 aagttcaaga gacttatact gaaaaacgtg agactgcagc gcaacggcaa gatgtgcctt
    16861 tccatattga acaattatct gaggcagaga caagaaagct ctttattgat atcgatctcc
    16921 gtttagcagg atggatattt gaagaaaact gtcgtgttga gatagccgtt gatggtctca
    16981 agcacggttc aggaattggt tactgtgact atgtacttta tggtaaaaat gggaaaattt
    17041 tagcgattgt ggaggctaaa aaagcctctg tcaatccaga agtaggggaa gtacaggtca
    17101 aagaatatgc tgaagctttg gagaaacata tcggctatca gccaatttgc tttattacaa
    17161 atgggttgaa gcactatata cttgatggtc cgaaccgccg ccagattgca ggcttttact
    17221 ctcaagaaga attgcaatta gtgatggata gacgtcatct tcaaaaaccg cttgaggata
    17281 tttctagtaa aattagggac gatatttccg ggcgtcacta ccaaaaacat gccattgcaa
    17341 gcgtttgtga agctttctct aatcatcgta gacaggcact tttggttatg gcaactgggg
    17401 cggggaaaac tcgtacagca gtttctctag ttgatatctt atcacgtcat aactgggtaa
    17461 aaaacgttct cttcttagcc gatagaactt ccttggttaa gcaagcctat gattcgttta
    17521 gaaaattact cccagatctt tccgtttgta acttcttaga agataaagaa ggagctcaat
    17581 caagtcgtat ggtcttttca acttatccga ccatgattgg agcgattagt ggtcaagaag
    17641 aagtaaatca acgccctttt actgttgggc attttgacct tatcataatt gacgaatctc
    17701 accgttctat ttatcagaaa tacaagtcca tttttgatta ttttgatgca agaattgtag
    17761 gcttaacagc tactccgcgt caagatttag ataaaaacac ctatggattc tttaatttgg
    17821 agaatggggt tccaacatat gcatatgatt tgaaagaggc tgttaaagac ggatatttag
    17881 tagcctatca ttctatcgaa atcaaactga aactacctac ggatggtcta cattatgatg
    17941 atttgtccga agaagaaaag gaacattttg atagcaaatt tgaagacgat agctgtgaaa
    18001 aagatattga tgggagtgta tttaattcct ttattttcaa taaaagtaca gtagaaattg
    18061 ttttaaatga actcatgaca agaggaattc agacagcctc gggtgatgaa attggtaaaa
    18121 ctattatttt tgctaaaaat catgatcatg cggaatatat cagaggtatt tttaacaacc
    18181 gctatcctga aaaagggagc gactatgctc aggtgattga ttatagtatt aagcattatc
    18241 agaccttgat tgatgatttt aaaattaagg agaagtatcc tcaaattgcg atttctgtcg
    18301 atatgttaga tacaggtatt gatgtaccag aggttgttaa tttagtcttc ttcaagaaag
    18361 tacgctctaa aactaagttt tggcagatga ttggtcgagg aacccgtcta tgtaaagatt
    18421 tatttggacc tgagcaggat aaggaaaact tcttggtatt tgattatggg gataattttg
    18481 attattttcg tgcagatcca agagatggag agggtcgtca cattgtttcg ttgactcagc
    18541 gtttatttaa tatcaaagtg gacttgattc gagaacttca gggactccaa taccaagaag
    18601 atcagtttgc gagagcatac cgtcagcagc ttgtctcgga actttaaggt cgtatagaga
    18661 gcttaaatga gttggacttc agggttcgta tggttttaga tacagtttat agctatagga
    18721 aattggaaag ttggcagaat ctaactgctg ttacaagtga aaccattcaa aaaaatctct
    18781 ctccgctttt atttgatgaa gataaagaag atgagatggc gaggagattt gatttgtggt
    18841 tgcttcatat tcagttgggg caactgacag ctaaatcttc cactgttcat atttcccaag
    18901 tgatgaagac ggctagagct ctttctgcta ttggcaatat cccgcaggtt tttgagcagg
    18961 ctgaaattat caggaaagta caggagcctg aattttggaa agaagttaac ttgtctgatt
    19021 tggaaaaaat tcgtcttgct attcgagatt tattacagtt tttggataaa acagaccgta
    19081 aaccctacta tgttaacttt gaagatcgta tactctccac tgttcacgag accacagcat
    19141 ttttgcaggt caacgatctt cggtcttaca atgaaaaagt tgagcattat ttgaaaactc
    19201 atctggatga ggagtccatt tctaagctat accataataa aaagttgaca tctgatgata
    19261 tgcttgcact tgaaaaattg ctttgggaaa aattaggtag taaagcagac taccaaagtc
    19321 attatgaaaa taaggcaatt ccgagattgg ttcgtgagat tattggctta gatagagagt
    19381 ctgccaatcg tattttttct aaatttttgt cggatgagaa tcttaatgcc aggcagattt
    19441 catttgtaaa attgattgta gactacattg tagaaaatgg ttttttagag acgaaagtgt
    19501 taacgcaaga gccgtttaaa tcttatggtt ctgttcaact actcttccaa caccaactac
    19561 cagtacttcg taatattgtt caaatcattg aacttatcaa taatcgagct ggagaagcgg
    19621 cttaaattct aaagtgattg ccatgttgag actcatttaa aattaaaaag agtagaaatt
    19681 tatgctatat atgagaagtt ttattaggaa gaatgtcatc gttttcctag aatacagtat
    19741 cagttgttaa gtggttgata aatttcaaag tagatacttg taccacgatg tttgttgatc
    19801 gagttattaa caaaagagct actttgattt taaagaaata gaaaacaaaa agccgagcaa
    19861 gaattcaatt gcaggagaaa atgaaataat actcaatgaa aatcaaagag caaactagga
    19921 aactagctgc aggctgctca aaacactgtt ttgaggttgc agataagact gacgaagtca
    19981 gctcaaaaca tggttttgag gttgtagatg aaactgacga agtcagtaac catacctacg
    20041 gcaaggcgac gttgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata
    20101 aagcaaaacg catagtatca agggttttca acacttgata ctatgcgttt tctgatgtta
    20161 aagactttct accagttttt tttaagcata attgttagtt gtagtcattt attattcttc
    20221 aaagaaaaat ggtggggcga attttttcag ttcttcaaag cacttttgag cagtatctgc
    20281 atcttcacag atgataagac agacatcatt accacaaagg gtagcgatag cgtcagggaa
    20341 gctcaaagta tcaatgatag aaccaaagga ttgagccagt ccaggaaggg tttttagtag
    20401 gacttggtgt tgaactgggc gcatccagac aagggcgtct tccatgtaga gttcgagacg
    20461 tttttcccat tttgagatgg aaccattgtt aagaacataa taagcgctat cttcttcgcg
    20521 gacttttgat aggttcatat ttttgatgtc gcgtgagagg gttgcctggg ttacttgaat
    20581 gtcgttctta gcaagaaggg cttgcaactc agcctgtgta tgaatcttgt tttttgtgat
    20641 aagagcgcgt ataagttggt ggcggtgttc tgatttattc ataataatgt aactcctttt
    20701 agcaaggtaa ggtaagcatg gactgagcga ggtcgacagt caagtggtag tctgtattgt
    20761 cacggatggt gatttcaaag tcagtagtat agaggactaa acggagagtg tctccttctt
    20821 ttagcttgta aatagttggc tgcagttcaa attgaacatc catccattca tctgcagtaa
    20881 tatcctctac taacagtaaa tcatttctat tttgtaaatt aaggtaacct tttgtcacga
    20941 ctcgttgtgc ctctggtcta aatggcaatt cacagagatt ttccaacatg tgatagcgac
    21001 cgttgtcaat ggttctagca cttaaaatag ctggataagg ttgtaggtat ttcttttgcc
    21061 caaattctag cagttgggca gataagagcc ccttgtttgt actggatttg atacgaagat
    21121 tgagctgagc gcgaccgttt aggtggagat ctttagtcac aggaaggtta atagtaatct
    21181 gattagcttt cccttgatag agctctgtat tgaaggtttg gtatgtctta ctatagcgct
    21241 caaaatcctt atctgggtac tggttttgaa tagcttgctc ttcttgacca agtgagaagg
    21301 ttttacagtt ttcttgccca ccgaagttat caagtgataa ccaagtctgt ggagctgtat
    21361 tgtcctgcca gataacagta ggaagttgaa agtctgtttc ctgtcctagt aattttttgg
    21421 ttaataaggc atttatggac tcacggaagt caattgattg ccaattgttc atgtaaacat
    21481 gggcaccatt atggaaaaag agatgcttgt gtatatgagt aggaagagca tggaacatct
    21541 ggtaaacatg aagtggtttg acattccaat cctgagaacc atgagtaaag acaacctctg
    21601 cctttacttt atgggcattg agcagataat tgcggtcatg ccaaaactga ttgtagtccc
    21661 cagtttttcg gtctagctga gctttcactt tttctaagtc agcttggtga gcttcattgc
    21721 cacggatata gtcgccagct aagagattac gagaataggt taactcagca agggagtcaa
    21781 agtcctcacc tggataacca cctgggctag tcaccagacc gttttcacgg tagtagttgt
    21841 accatgatga aattcctgcc tcggcaatga taacttctaa accatcgact cctgtagtcg
    21901 caagaccatt ggacatggta cctagatagg aaagtcctgt tgtagcaact tttccgtttg
    21961 accaatcagc cttgacttga cgctggcgcg tgtgatcagt aaaggcacgg caacggccgt
    22021 taagccaatc gatgacattt ttataagcct cgatttgctg gtagtctcca ttagtcatga
    22081 aacctgtcga gtctttggta ccaacacctg agacatagag attggcaaag cctctcggaa
    22141 ggaagtagtc gtttagtgta tagctagagt tgatgtgagt tagcttttcc tcagcctctg
    22201 ctataagctc agctttacct tggggttgga cgagatttag ttgaggttcc tctagctcaa
    22261 tcttgtgagg aagcttaacc tcaagctcaa cctccatctt gtagagagcc ttgtcactag
    22321 ccttgtcact agccttgtca ttggttccct gatgataagg gctggctgtc atgatggcag
    22381 ggatttttcc atcaaaacga gggcgaataa tgctaacctt tactaggtct gatagccctt
    22441 tttggtcagt atcgacacga gactcaacgt aaacgacttc acgaatgaca tcctggttag
    22501 aaaaagtagc caaactcttg ccgttaaagt agtggtagtc attatcctcc ggaataagac
    22561 catcactaac aagttggtcg ataagagtat ttcctttttt ggtgcgagta ttgagtaact
    22621 gatagagatt ttcaatcaag tcaccatata taatgggaaa tccagtttct ttacgaaaaa
    22681 cgtcactatc ttcgaagtca accaaataag aaaagcctaa aagttgaaaa gcaacagtat
    22741 aaaaaatatc tgctgtcagt tcatcttctg attgaaaaaa tgtcagcagg tctgtttttt
    22801 tatcagctgc taggatagaa agtgggtagt tggtgtcttg ataagtgaaa aagaaacgac
    22861 gtaaaaaggt ttcaagtgag tctttgtgat tggctgtatt ttgtaaatca aagccacatt
    22921 tttttagttc agataagaca ttttcttttg gaaaattgat ataactatat tgattaaaac
    22981 gcatagaacc tccatataga atgacagtta aggttattat atcaaaaaaa aagcagaaag
    23041 ggaattgtta acttcaaaag gaaataatcc aataaaaatg aataaagtac taaattcaat
    23101 atagagaaca gag
//
LOCUS       DAVLVZ010000025        23032 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000025 DAVLVZ010000000
VERSION     DAVLVZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23032
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.025"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(166..537)
                     /locus_tag="VYN77_001036"
     CDS             complement(166..537)
                     /locus_tag="VYN77_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEV1558508.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
     gene            complement(515..853)
                     /locus_tag="VYN77_001037"
     CDS             complement(515..853)
                     /locus_tag="VYN77_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1558509.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            1319..2182
                     /locus_tag="VYN77_001038"
     CDS             1319..2182
                     /locus_tag="VYN77_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1558510.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLVQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            complement(2234..2821)
                     /locus_tag="VYN77_001039"
     CDS             complement(2234..2821)
                     /locus_tag="VYN77_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1558511.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            3063..3734
                     /gene="sdaAB"
                     /locus_tag="VYN77_001040"
     CDS             3063..3734
                     /gene="sdaAB"
                     /locus_tag="VYN77_001040"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1558512.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            3743..4615
                     /gene="sdaAA"
                     /locus_tag="VYN77_001041"
     CDS             3743..4615
                     /gene="sdaAA"
                     /locus_tag="VYN77_001041"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1558513.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            4637..5269
                     /locus_tag="VYN77_001042"
     CDS             4637..5269
                     /locus_tag="VYN77_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1558514.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            5319..5486
                     /locus_tag="VYN77_001043"
     CDS             5319..5486
                     /locus_tag="VYN77_001043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558515.1"
                     /translation="MKIKEQTSKLAAGCSKHCFKVVDRTDEVSSKHCFEVVDRTDEVS
                     NHIRQGDVDVI"
     gene            complement(5572..7422)
                     /locus_tag="VYN77_001044"
     CDS             complement(5572..7422)
                     /locus_tag="VYN77_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1558516.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(7464..8621)
                     /locus_tag="VYN77_001045"
     CDS             complement(7464..8621)
                     /locus_tag="VYN77_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1558517.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            8679..9845
                     /locus_tag="VYN77_001046"
     CDS             8679..9845
                     /locus_tag="VYN77_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1558518.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            9984..10310
                     /locus_tag="VYN77_001047"
     CDS             9984..10310
                     /locus_tag="VYN77_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1558519.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            10297..10890
                     /locus_tag="VYN77_001048"
     CDS             10297..10890
                     /locus_tag="VYN77_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1558520.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            10883..11794
                     /locus_tag="VYN77_001049"
     CDS             10883..11794
                     /locus_tag="VYN77_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1558521.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDFITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            11856..12920
                     /locus_tag="VYN77_001050"
     CDS             11856..12920
                     /locus_tag="VYN77_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558522.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(12992..13852)
                     /locus_tag="VYN77_001051"
     CDS             complement(12992..13852)
                     /locus_tag="VYN77_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1558523.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKLEDYESIARLDYAKFLEVLPLSFYHQLDANQLEIQPILG
                     QDFKTLAQEK"
     gene            complement(14127..15113)
                     /locus_tag="VYN77_001052"
     CDS             complement(14127..15113)
                     /locus_tag="VYN77_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1558524.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(15385..16860)
                     /locus_tag="VYN77_001053"
     CDS             complement(15385..16860)
                     /locus_tag="VYN77_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1558525.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(16964..17887)
                     /locus_tag="VYN77_001054"
     CDS             complement(16964..17887)
                     /locus_tag="VYN77_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1558526.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(17901..18830)
                     /locus_tag="VYN77_001055"
     CDS             complement(17901..18830)
                     /locus_tag="VYN77_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1558527.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(19149..19370)
                     /locus_tag="VYN77_001056"
     CDS             complement(19149..19370)
                     /locus_tag="VYN77_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558528.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(19324..19584)
                     /locus_tag="VYN77_001057"
     CDS             complement(19324..19584)
                     /locus_tag="VYN77_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558529.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            complement(19980..20591)
                     /gene="rpsD"
                     /locus_tag="VYN77_001058"
     CDS             complement(19980..20591)
                     /gene="rpsD"
                     /locus_tag="VYN77_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1558530.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(20786..21838)
                     /locus_tag="VYN77_001059"
     CDS             complement(20786..21838)
                     /locus_tag="VYN77_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1558531.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(21835..22533)
                     /locus_tag="VYN77_001060"
     CDS             complement(21835..22533)
                     /locus_tag="VYN77_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1558532.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(22700..>23032)
                     /locus_tag="VYN77_001061"
     CDS             complement(22700..>23032)
                     /locus_tag="VYN77_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1558533.1"
                     /translation="INVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT         6854 a         4982 c         4236 g         6960 t
ORIGIN      
        1 gctatcaata tttcaattgg taaaaaatag taattccaaa taaaatgaga aatcagcaat
       61 gatagaaaaa taacaatatc aattttcctg ttgttgacat actctgtaat tttatgatac
      121 aggaatgcaa caataactga aattaaaaca accatcataa tactattatc ctattctcaa
      181 ttgattatca cgtcgcccat cccagccatc aataatattg gtaaaaatcc aatcagctcc
      241 agcaccaatt ccagcagatg caatacttct aataacagta tttgcaaaag catttccaat
      301 atgaattcct aatactccta aacctttaac aatcccagct accagtgcgg agcgtaatcc
      361 ataagatgca agaactagtt gaaggcctcc acctcctgct gctgctaatg ctgcatatgc
      421 tgcaccagta atggctcctg taattgccca cctgggtata tatatagccc ccccatcaag
      481 gtacatcatc tcttcttcat caatcacaac ataattattt ggtaatacga gttccattta
      541 ttttctcctt tatactatgt aataccacaa aatgattttg acctaaatca ttcttaactt
      601 gaagaatcgc aggaaatgtt aattttggag tcaaatcttc cagtgctact cgaacagctt
      661 ttactgtaaa attcaactta ttcagacctg aaacaatacc actgacagtc gttccataca
      721 tgtctgtacc aataatttcc ctaatcttca tgattgatag ttcctttttg taagataaaa
      781 gaactgttga aacaactgcc gcagcacaat ctgactggtc atgttgcatc gtacaaggat
      841 atttttttaa catagaaata ctcaattctt tagaattaat ctaacttttc tttgatgtca
      901 cccaaacctc aatcactgcc cagtagttct tgaattagtt tattttcaac cgtatcattc
      961 tttgttcttg atatctttcc aagcattgta gtacagcaga tttttgccca aggactgagc
     1021 ctcctttaac aactcaagct cccttacatt atatcggcca gccccatgga aagattatag
     1081 ccagactaat caatgatata atacatacta gaatacaaaa tattttttta ttcatacttt
     1141 ttctcctatc tttattttct acctacatag tacctcttat taagctcatt tcaaaattac
     1201 gttaactttt ttcacaaatt tatttcgact ttctttttta ctttttacat tgctatgata
     1261 caatattttt aacaaaattt tattgtttgt taactttttt cacgaaaagg agaaaatcat
     1321 gcgttatgat ttcggaaagg tctataaaga aatacgtgag tcaaaggggc tgacccaaga
     1381 agaggtctgt gggggtgttc tctcaagaac cagcttatca aaaatcgaaa gtggcaagac
     1441 aaccccaaaa tatgaaaata tggaatttct tctccgtcaa atcaatatga gttttgaaga
     1501 gtttgaatat atctgtcaac tttatcaacc aagtcaacgc acagagatta tgcaaaccta
     1561 tctcaatatg cgttccatta tcgggaccag cgatcttgtc aatttatttc aaaaatgcca
     1621 agactacctc aagacccacc acgatctccc tatagaagaa atcagagata tgcttgaagt
     1681 tgtcatttat atccgtcaac acggggcagg ggaactatca gatcacgcag aacaggttgt
     1741 caaaaaactt tggagaaaaa ttgaaaaaca agatacatgg tatgagagtg atctaaaaat
     1801 cctcaatacc attcttttca gttttcccat tgaatacctc catctcatca ctggaaaaat
     1861 cttgcaacgc ttagaagtct ataaaaacta tcaacattta tatgacttgc gaatagcaat
     1921 cctgctcaac ctttccacac tctatctata caatcaagat aaaaacatgt gtaagcaaat
     1981 ctgctacact ttactagagg acgccaagaa taagaaaagc tacgataggc tggctatctg
     2041 ctatgtccgt attgggattt gtacagacga ttctaaactt gtccaaaaag ggttctccct
     2101 tctggagcta accgaggaaa cttctatgct gtctcatctc aaaaaagaag tagagatcta
     2161 ttaccaagcg aaggaaagat aaaaaagccg agggatttcc tcgactcttt acatattatt
     2221 tgttcgttag ttcttaatac cagccattgt taagccagaa gtttttagca gcagtccatg
     2281 aaccgtaacg tcctgcaacg taggcatctg ctacacgttc ttggttttca gctgagtagt
     2341 caccgttcag gtatgaatct gttaattggt aacgtccgat ataacgtcca tttgtagctg
     2401 tatagctacc acctgattct ttttgagcga tccattcttt ggcttctgct tcagatccgc
     2461 ttacagttga aacaactgtt tcacttacta ctggagtttc tgctactgga gctgaaacag
     2521 tttcatcttg agcggctggt gccgcataag tagctggcgc tggtgttgca acaggcgcta
     2581 cagggccatc gataaccaac tcttgatcaa cataaatcaa atgaatgtta tcaatgtggt
     2641 tgttttctgc caatttttca actgttgtgt tgtgagtttc agcgatttct gaaagtgtat
     2701 caccttcttt aacagtgtaa gttgatgatt cttgagcaga tacaaatgat ggagcaaata
     2761 ctgcaaacaa ggcagctact cctgcaagag ttgctttaat ctttttagtt attgatttca
     2821 tattcgaaaa ttctccttct ttctatagtt ctatcatacc ctttaaatat taccgtttct
     2881 tgacactttt atgtagaaat attacaaaat tattttttat ttccctaaat aaggtgtttt
     2941 ttgtcataaa agatactatt tttatgctat ttgaggtatt agaaaggttt tcatccacaa
     3001 ttaaagccaa gcccctcatt ctttgatata atagagataa gaatttttat aaggggaaac
     3061 tgatgaaatc acttcgtttt caatctgtct ttgatatcat cggaccagtt atgattggcc
     3121 catctagtag ccataccgct ggtgctgttc gtattgggaa gattgtctct tccatttttg
     3181 atgatactcc gacagaagtt gaattccaac tttttaactc atttgccaag acctatcgtg
     3241 gtcacggaac agacctagcc cttgttgcag gtattttagg aatggataca gatgatcctg
     3301 aaattccaaa tagtctggaa attgcccaca agcgtggtat caagattgtc tggaccattc
     3361 agaaagacag caatgcccct caccctaaca ccactaaaat tactgttaaa aatgcccaca
     3421 agaccatcag cgtgactggt atttctatcg gtggaggaaa tattcaagta accgaactca
     3481 atggttttgc cgtctctctc aatatgaata caccgactat catcatcgtt catcaagata
     3541 ttccaggtat gattgccctt gttacagagg cgctttcccg ctatggtatc aatatcgccc
     3601 agatgaatgt cactcgtgaa aaagctggtg aaaaagccat tatgattatc gaagttgaca
     3661 gtcgcaactg tgatgaggct atcgaagaaa ttcgaaaaat ccctcatctc cacaatgtca
     3721 atttctttaa ataggaggaa gcatgtttta ttctatcaaa gaattggtcg agcaagcaga
     3781 tctggacttt caaggaaatg tcgcagaact catgattaca acagagtttg aattgaccgg
     3841 tcgcgaacgt gaagaagtct tccttctcat ggaacgcaat ctggaagtta tgaaagcctc
     3901 tgtccaactt ggcctcaatg aaaataaatc tcgtagtggc ctgacaggtg gagatgctgc
     3961 caaattggac cactacatca aaaacgaaaa aactttatca gattacacga ttctctctgc
     4021 tgcccgaaat gccatcgcag tcaatgaaca caatgccaaa atgggcttgg tctgtgccac
     4081 tccaaccgct ggaagtgctg gctgtctgcc atccgttctc actgctgcta ttgaaaaatt
     4141 agacctcagc cacgagcaac aattggattt cctctttgct gctggtgcct ttggactagt
     4201 catcgcaaac aatgcctcca tctcaggtgc tgagggtggg tgtcaagctg aagttggttc
     4261 agcctctgct atgagtgctg ccgccttgac tctggctgca ggtggaacac cttatcaggc
     4321 cagtcaagct attgcctttg tcattaaaaa tatgctaggc ctcatctgtg accctgttgc
     4381 aggtttggtc gaagttccct gtgtcaaacg taatgccatg ggagctagct ttgctttcat
     4441 cgcagcagac atggccttgg caggtatcga atctaaaatc cctgtggatg aagtgatcga
     4501 tgccatgtac caagtaggag caagcatgcc aactgccttt cgtgaaacag ctgaaggtgg
     4561 actcgctacc acccctactg gtcgtcgcct ccaaaaagaa attttcggag aataagctta
     4621 tcaaatagga gaaaccatga cctctatcac agcgattttt ttcgatttgg atggaaccct
     4681 cgttgatagt tctatcggga ttcacaatgc ctttacctat acctttaaag agctgggggt
     4741 gcctagccct gatgccaaaa ctattcgtgg ttttatggga ccacctctcg aaagtagttt
     4801 tgcgacctgc ctgtccaaag accaaatttc tgaagccgtg cagatatatc gttcttacta
     4861 taaggcaaaa ggcatctatg aagctcaact ctttcctcag attatagact tgcttgagga
     4921 gttatcgagc agttatccac tctacatcac cacgacaaag gatacatcta ctgctcaaga
     4981 catggcaaaa aacttggaaa tccatcattt ctttgatggc atttatggtt ctagccctga
     5041 agcaccccat aaggcagatg tcattcacca agccttgcag acacatcaac tagcaccaga
     5101 acaagccatc atcatcggag ataccaagtt tgatatgctg ggagctcgag aaacaggtat
     5161 tcagaaattg gccatcactt ggggatttgg agagcaagca gatctactaa actatcaacc
     5221 tgattatatc gctcacaaac ccttagaagt tttggcgtat tttcaatagc atagactggg
     5281 taacaattcc atttcagaag aaaatgaagt aatactcaat gaaaatcaaa gagcaaacta
     5341 gtaaactagc tgcgggctgc tctaaacact gttttaaggt tgtggataga actgacgaag
     5401 tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagt aaccatatac
     5461 ggcaaggcga cgttgacgtg atttgaagag attttcgaag agtataagtc tctacataat
     5521 aaaacgcata ttaccaaggt tgtttaactt gataatatgc gtttttcttt tttattcaat
     5581 gtgggttgtt tgattagcaa aggcgataat agcttgcttc aactcatctc cactgagagt
     5641 gcggaactct ccaatctttt gattgatgga ttctaaaggc atgacattaa ccttaccaac
     5701 gaaaatctta tccataactt gattgtcaac gagttcggtt gataccaaga gttcttcgta
     5761 gagtttttca cctgggcgga ttccaacttc aacgattgga atttcacttt cagtgtggcc
     5821 acttagaagc accatcttct tggccaagtc ataaatcttg actggtttac ccatatcaag
     5881 gataaagact tccccatctt tggcataagc accagcatgg ataaccagac ggctagcttc
     5941 tggaatggtc ataaagtaac gggtcatgcg gaagtctgtc accgttacag gcccaccttc
     6001 agcaatttga cgttcaaaga ctggaatgac actaccacgg ctaccaagaa cattcccaaa
     6061 acgaactgca cagtaggttg attggctacg ttggttaaag ccagtgacaa tcaactccgc
     6121 cacgcgcttg gttgctccca taacatttgg tggattgact gccttatctg tcgaaatcat
     6181 aaccatctta ggcaccttgg cttcatcaac ggctctagca acattgtaag ttccacggat
     6241 attgtttttg aaggcttctt ttggattgcg ctccatcata ggaacatgct tgtgggctgc
     6301 cgcatgataa acaatagcag gtttgtactg ctcaaagact tgcaacaaac gatcatagtc
     6361 ttgaatgtcc gcaatcacag gtacataatc aatcccttgg aacttacgaa tcaattcatg
     6421 ataaacaagg tagattgagt tttccccatg accgagcaag acaatgcgtt caggattgaa
     6481 gcgactaact tgacgacaga tttcagaacc gattgaacct ccagctcctg tgactaagat
     6541 ggtcttacct gtcagttctg cacccagacg cgattcgtca agacggattt cctgacgacc
     6601 caaaaggtcc gtaatatcaa ttttttggaa gccagtacct gcttggtgaa ggccctgaac
     6661 aacagtttca accttaggca tcttgtaaca tttgacaccc agcttattac acatctgcaa
     6721 gatacgctca tattctgacg gatccagcga cggaatcgca acgatgacac gctcgatttg
     6781 atggcgtttg gctaattcag gcagattgtc ataagagccc aaaacaggaa taccaccaag
     6841 tttttgaccc tttttcttag aatccttatc caaaataccg accagttcta attcactggt
     6901 tggatgttgg taactatcca taaaaagagc cccaccatca ccggcaccaa tcaagaaggt
     6961 ccgacggtgt tctccatcac cactaccttt tttgcgtctg gagtagatta actgccaagt
     7021 aatccgtggc aataaaatca agaaggtact caacaagata aagagaatga tgaaacggat
     7081 ggagaagagt ggcaagaagg cataacagat actatatgac aagacactgc tagcagtcac
     7141 accaaaaaag attttcatga aatccgtaat cttgctgtaa cgactaatgc tcgcgttcaa
     7201 cccccaaaaa ccaatcatca attgatagaa caagaaggcc aaactcgtat agataatgta
     7261 gtcaacaggt gctggattaa tcagcccata aaataaaata taagatacaa ttatggaaac
     7321 caccatactg aaaatatcaa atattcccca gaaaacctgc ttttgttgtt tatttaaaat
     7381 ttccaccaga tcaatcacat aatctgttag ttttttattc atagaaatct actcctcctt
     7441 aaaagagttt tactatattg ttattatagc atatttttat ttatttggct tattttatcc
     7501 aaataaatta ttgttctctt gtactccttc ttccatttaa atcctctaat taaacctaca
     7561 atcgtcccaa ggccataagc aaagtgaatg gaaaataaaa gaaagggcat cacaattaga
     7621 aatccatttt tatgttttaa taaagtcagc aaagtgagta atgacaaaag tagaaaatag
     7681 gcacctaata gtaaagttat gaatacgaat gtgatcggta acaatgctag actaaacaca
     7741 agactcaaaa caaataaaca aggaacatag tgaaataatg ataaacactt aaactgaaca
     7801 tgacttgtca agccaatcca caaaccattt gaatactttt gatgcagcat tttcttgaat
     7861 gttggtcgaa tatactgata agatagaata cttgggctat agcggatttt ataaccatgt
     7921 tctcgaattc tataatgaat atcattatct tcagttcggc caagttgctc atttactaaa
     7981 ccaaccttct ggaaaacctc tcgtttatac atcccatgaa aaatagaaga aacatatcta
     8041 tcctcggaac tatttcgata attggcaatg ctactgccaa acatattttc ctcaacaaga
     8101 tgcaaggtct ctgcccattt tccttttcct tcgacaatcg tcggtctagg ccccccacag
     8161 acaaattcac cttgttgaat aatagccaca ttgttcatta caaaactctc agtaactttt
     8221 gaatgagcat caatttttaa aataaggtcc cctacagaat gtttaactcc caggttaaaa
     8281 ccactagctt gatttttctt aggattgtta tacaatctaa ttgagttaaa ctctgtatct
     8341 tcctttataa attgctgaat gatagctgtg gtcccatctg tggacatagc atttataaat
     8401 agaatttcaa tattctcttt aggataggtt tgatttttta agtcttcaat tagaccaggc
     8461 agatattttt cttcattgta tgcactgatt acaatggata cgagaagact ttcaacattt
     8521 tgagtcacta ctactctcct gaatttttcc ttccaccatt ctatcataat tttcaagact
     8581 ttcccatttt tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa aaagccttaa
     8641 aataagctgt tattaaaatc agctagaaga ggtattatat gaaaaagcaa tcactctttt
     8701 ttgttccagg tattatcctg attggtgttt ccttgcgaac tccttttact gttttaccca
     8761 ttattttggg aaatatttcg caaggtctgg aggtagaagt tagttcgctt ggtgtcttga
     8821 ccagcctgcc tctccttatg tttaccctct tttcaccatt ttctacccaa ctggctcaga
     8881 aaatcggctt ggagcatctc ttcacctaca gcctcttctt cttgaccatc ggctcactta
     8941 ttcgactaat caatctgccc ctgctctatc taggaacctt gatggttggg gcaagtgtcg
     9001 cagtcatcaa tgtcctgctt cctagtctta tccaagccaa tcaaccaaag aaaattggtt
     9061 ttctgaccac cttatatgta acgtctatgg ggattgcaac ggctctggct tcctatctag
     9121 ctgtgcccat tacacaagcc agttcttgga aaggacttat ccttctcctc acgttactct
     9181 gtctagcaac ttttttggtc tggctcccaa atcaccgcta taatcataga ctagctccac
     9241 aaaccaaaca aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg gcaattatta
     9301 tcttttcagg ttttcaatcc ttgatctttt acaccgtcat gacctggtta cctaccatgt
     9361 ctatccatgc aggtctatcc agtcacgaag ctggcttgct gacttctatc ttatctctga
     9421 ttagcattcc tttttcaatg accatcccaa gcctgacaac cagtttatct actcgcaacc
     9481 gtcagctcat gctcactttg gtttcactag ctggtgtggt cggcatttcc atgctctttt
     9541 tcccaatcaa taatttcatt tactggctta ccatccatct cctcatcgga accgcaacca
     9601 gcgccctctt cccttatctc atggtcaact tttcactcaa gacaagcgcc cctgaaaaga
     9661 cagcccaatt gtccggccta tctcaaacag gaggctatat cctagcagcc tttgggccaa
     9721 ccctctttgg ttacagtttt gacctgttcc actcttgggt accagctgta gctgccctct
     9781 tgctcatcta tatcctgatg actgtggccc tctttacagt ggacagagcg gataagatcc
     9841 tttaaacttc tagttttagc tagaagtttt ttatatataa aaattttaca catttctctt
     9901 gacagggctt tcttttagat gtacaatgta tgtgtagaaa aattatatat aaaaatctta
     9961 cacattagaa aaggaggttt cccatgtact ttccaacatc ctctgccttg attgaatttc
    10021 tcatcttggc tgtactggag cagggtgatt cttatggtta tgagattagc caaaccatta
    10081 agctgatcgc taatatcaaa gaatccacac tctatcccat tctcaaaaaa ttggaaggca
    10141 atagctttct gacaacctat tctagagagt tccaaggtcg catgcgcaaa tactactcct
    10201 tgacaaacgg tggtatagag cagctcttga ccctaaaaga tgaatggaca ctctatacag
    10261 acaccatcaa tggcatcata gaagggagta tccgccatga caagaactga atacctgact
    10321 cagctagaac tctatctcaa gaaactacct gaagctgacc gtatcgaagc catggactat
    10381 ttcagagagc tctttgacga tgctggagtc gaaggagaag aagaactcat cgctagtttg
    10441 ggaactccca aagaagcggc ccacgaagtt ctatccaatc ttctcgataa aaaaatcaat
    10501 gaagcacccg ctcaaaaaaa taaccgacaa attttacata tcgccttgtt agccctcctt
    10561 gcagcaccta tcggcattcc tctgggaatc gccatcctcg tgaccctgtt cgcaatcctt
    10621 gtagccgctt tgactgtcat tctggctttc tttgcagttt ccatactggg tatcatcggc
    10681 ggattcctat ttttagttga aagtttcact atcctcgccc aagccaaatc agcctttatc
    10741 ttgatttttg gttctggttt actggctatc ggtgcttctt cgctagtttt acttggcatt
    10801 tcctatgtag ctcgcttctt cggtctactc attgttcgtc tggtacaatt tgttcttaaa
    10861 aaaggaaaga gaggtaatca gcatgcgtaa atggacaaaa ggatttctca tctttggtgt
    10921 ggtgactacc gttatcggct ttatcctgct ttttgtaggt atccaatctg acgggattaa
    10981 gagcctactt tccatgtcca aagaacctgt ctatgatagc cgtacggaaa agctaacctt
    11041 tggcaaggaa gtcgaaaacc tagaaattac tctccaccaa cacgcgctca ccatcacaga
    11101 ctctttcgat gatcaaatcc acatttctta ccatccatct ctttctgctc accatgattt
    11161 tatcaccaat cagaacgata gaactctgag tctcactgat aagaaactgt ctgaaactcc
    11221 gtttctctct tctggaattg gtgggattct tcatatcgca agtagctact ctagtcgttt
    11281 tgaagaagtt attctccagc taccaaaagg gagaactcta aaagggatca acatctcagc
    11341 caatcgcgga caaaccacca tcataaatgc tagccttgaa aatgcgaccc tcaatacaaa
    11401 cagctatatc ctccgaattg aaggaagtcg tatcaaaaac agtaaactca caacgcccaa
    11461 tatcgttaat atctttgata cagttcttac agatagtcag ctagagtcaa cagagaatca
    11521 cttccacgct gaaaatatcc aagtccatgg caaggttgaa ctgactgcca aagattatct
    11581 cagaatcatc ctagaccaga aagaaagcca acgaattaac tgggacatct caagcaacta
    11641 tggttctatc ttccaattca caagagaaaa gcctgaatca agaggtacgg aattaagcaa
    11701 cccttacaaa actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat ctgatgataa
    11761 tattgatcta atatccacac caagcagacg ttgacaaaaa cgcttacatc tgctgctatg
    11821 gaagcatatt tatctaacca aaaggaggtt ctaccatgaa acaagaatgg tttgaaagta
    11881 atgattttgt aaaaacaaca agcaagaaca agcctgaaga gcaagctcaa gaggttgcag
    11941 acaaggctga agaaacgata gccgatctcg atacaccaat tgaaaaaaat actcagttag
    12001 aggaggaagt ctctcaagct gaagtcgaat tggaaagcca gcaagaagag aaaattgaag
    12061 ctcctgaaga cagtgaagcg agaacagaaa tagaagaaaa gaaggcatct aattctactg
    12121 aagaagagcc agacctttct aaagaaacag aaaaagtcac tatagctgaa gagagccaag
    12181 aagctcttcc tcagcaaaaa gcaaccacga aagagccact tcttatcagt aaatctttag
    12241 aaagtcctta tatccccgac caagctccaa aatctaggga taaatggaaa gagcaagtgc
    12301 ttgatttttg gtcttggcta gtggaagcga tcaaatctcc tacaagtaag ttggaaacaa
    12361 gtatcacaca cagttacaca gcctttctct tgctcattct gttttctgca tcttcctttt
    12421 tctttagtat ctatcacatc aaacatgctt actatggaca tatagcaagc attaacagtc
    12481 gcttccctga gcagctagct cctttaactc ttttttctat catctctatc ctagtagcga
    12541 caacactctt cttcttttca ttcctcttgg gtagtttcgt tgtgagacga tttatccacc
    12601 aggaaaagga ctggacgcta gacaaggttc tccaacaata tagtcaactc ttggcaattc
    12661 caatcttcct cactgctatt gctagtttct ttgctttctt tgatagccta cgatttacag
    12721 ccctcttgtg tgtgattagc attggaatca ttctgcttgc cagtctccat atcattacaa
    12781 gaccaagtca agcaagtgaa accgactcct tctatcaatt attcttgtct gtccttgtga
    12841 acggagttat tatcctcctc ttctttgtag ctgaagtggc actgattgga gattatcttc
    12901 gtatcttggc ctttctttaa aattcaatcc tgtcatcctt ggcaggattt tttctatgtc
    12961 aaaaaagcaa gtcgattgac ctgcttctgc tttacttttc ttgtgctaat gttttaaaat
    13021 cttgtcctag gatgggttgt atttctaatt gattggcgtc tagttggtgg taaaaagata
    13081 ggggtaacac ctctaagaat tttgcatagt ccaagcgagc tatggattca tagtcttcta
    13141 gtttagagcc gtctcctgtg atttgaacca agtcaatctc ccactggact tccttatcca
    13201 ccaattgctc aatataggct gcaggaacaa acggttctct cggtaaaact gtcttgactc
    13261 cttgagctag atggtaaacg ccatgcactt ttccttctcc atcctgatag aaggaaactc
    13321 tcgcaaagta ggcatctgtc tcttgaaccg cacgcacacg cgcttcaaac tgagccaagc
    13381 catctgcctc taaaaagctt ttcaagttct cacgactaaa gaaaatagga ttaaaaattc
    13441 cttggcaaat cgtaaaacga gctacagggt ctgctagata ggcttgaata cttgcttgga
    13501 aataagcttc aaaatcaaaa ccatctagcc cttcaatatc agtccctgta taagcaagca
    13561 aagcatctaa aaatgacttg ataatctgcc agtctgtctt tgttagtagg tcaggaagat
    13621 cgacacggta agccttttga ccatcagcat aactgacctt aaagagaaat tgtgattgcc
    13681 ccactatcgc acactcgata tattcgagac gattaagagg ctgacggaga tagactacat
    13741 catagctatg tgactccaaa ccgtctagca aggccaagat cgacttggca ttcaaaatct
    13801 cttgttctcc taaaatgctc tgtttatttg gaataaaaaa tgttttcgcc ataactggac
    13861 tcctttgaaa tcgtttacat acagtatacc ttatttttct gaaagataca aaaacaaact
    13921 ttatactctt cgaaaatctc ttcaaaccgc gtcagcttcg ccttgccgta gatatatgta
    13981 actgacttcg tcagtcttat ctacaacctc aaagcagtgc tttgagcagc ctgcagctag
    14041 cttcctagtt tgctctttga ttttcattga gtattagatt tttgagtaca aagcatagaa
    14101 aaacagcccc tgaggactgt ttgatcttat acagtagctg cttgatccaa gctttcaccg
    14161 atagcggcta ggcgctcgat aacttcagct tgtgtcaatt cattttttga aacatagcgg
    14221 ttacgtgggt gaacacggca ctcgtgtgag catccacgaa ggtacttgtc ttcattttct
    14281 tctgatgtca agatacgacg gttacagaag ggatttccac agttgacata acgttcacat
    14341 ggtgttccat caaaccagtc tttccctacg atagttgggt tgacatggtt gacatcaacg
    14401 gcaatacgct cgtcaaagac gtacattttc ccatcccaaa gctcaccttg aacttctggg
    14461 tctttaccgt aagttgcgat tcctccgtgc aattggccga catctttgta gccttcacgg
    14521 accatccagc ctgagaattt ctcacagcga acgccacctg tacagtaaac cacgacacgc
    14581 ttgtccatga atttttcttt gttgtcacga acccattgtg gtaactcacg gaagttgcga
    14641 atatctgggc gaatagctcc acggaaatgt cctaggtcgt actcataatc gttacgtgtg
    14701 tcaaggacaa cggtatcttt atcaagaagc gcttctttga actcttttgg agacaagtaa
    14761 gcacctgttg tttcaagtgg gttgatgtca ttgtcaaagt cgttgtcttc caaaccaagg
    14821 tggacaattt ctttcttgta gcgaacaaac atcttcttga aggcttgttc attttcttcg
    14881 tcaatcttga accagagttc ttccatacct gggaggctgt gaacgtagtc catgtatttt
    14941 tgagttgttt catagtcacc tgaaactgtt ccgttaattc cctcgtcagc gactaggata
    15001 cggcctttaa ggccgattga tttacagaaa gccaagtggt ctgcagcaaa ttgctctgca
    15061 ttttcaattg gagtataaag gtagtaaagt aagacacgaa tatcttttgc cataagattt
    15121 gttctctttt ctattcttaa attttctaaa ttttttattt atccatacta gtatacctgc
    15181 ttgagaaaac gaatgcaatt tatttgactg gaaattgtag aggattctcg aaatttcctg
    15241 aatggttaaa ataaggacaa gagaaaacag ggatatctat atccttgtgc caaaaaaacc
    15301 actgccctcc ccagaccaac ctgaggaaag cagtgattct tattttagga gttaggaatg
    15361 aatacacgaa atcaatttag ctgattattt tttgtttttc aagaattcat cgtattgttt
    15421 ttgcatttcg ttcaatactt tttcgtaggc accttcagat ttcaattttt ccatcaattc
    15481 tggaatcgct ttatctgggt ctacagtacc agtgttgata gctgtatcaa attgttgcat
    15541 tgtgttagca atagctgaga tttcagattt cacattgtca gtattgaaga taaatccaag
    15601 cgctggagat tctttagctt ctgccaattc tttcttagaa ttttcgattt gttggtctgt
    15661 aacgttttcg ttgatgtaaa ggatccagtt gttaccagtg ttccatccac ccatgtgagt
    15721 gtttcctttg tagccatcaa gaacgcgaac acggttttct ttaccttcaa ttttttccca
    15781 gttcttgcct tctggaccgt aaacaagacc gttcaagagt tctggattcg tattcaagag
    15841 gttcaagatt tccattgatt tttctttgtt cttagagttg tttgagatga caaagttagc
    15901 aacttgtgtt gtttggtttt tcttgatgaa gttagtaatt ggtttgattt ggatatcttt
    15961 gttggcaaca cgtgaaagca agctgttacc gtagtcagct ggtcctactg tttcttcacg
    16021 aacgaaccaa gtatcttgtt gaaggtcaaa ggaagtatcg cttgttgcga cgtcttttgg
    16081 aatgtagcca gcttcataga atttgtgaag agtcttcaag tgttctttga aacgaggcac
    16141 ttcgtaacgg tttacaactt tagtagtatc gccttcaagg tcaataacga atggaagccc
    16201 gtttgctact gggtagtcaa aattatcaga tgggatgaaa actttaccaa tagcaaatgg
    16261 tactacgtct ggagcttttt ctttgatttg tttcaagact ggctcaagag tttcgtaaga
    16321 agtaacacct gaaatatcga taccatattt ggcaaggaga gttccgttga aggcaaagtt
    16381 ttgagatgat gcaacgttgg ctgcaactgg tacagcataa atcttaccat ttacagtatt
    16441 acccttgata taagctgggt caagtgcctt gtaaaggtct ttaccttctt ttttgtacaa
    16501 ttctgtcaag tcagcataag cacctttttg agcatttaca atatagttat ctgcaaaggc
    16561 gatatcatag ttttcaccag atgatgtgat aactgacatt ttcttaccat agtcacccca
    16621 gccaaggtat tggatatcca atttagcacc aactttttct tcgatgattt tgttggcatt
    16681 tgctaacaat tcatccaagt tatctggttt gtcaccgatt tggtacattt tgataacagg
    16741 tttgtcacct gaatcagcag cttttttact gttacctgtc aagttgccac aagcagcaag
    16801 acctgcagcc aaagcgacta cactagcaga tgcaaaagca tattttttcc agtttttcat
    16861 gataaaaact ccttttttta tttttaaact tataaacaat gtaatgatct taacttcacg
    16921 caagggaagt ttggaaatga gaaatgttct ttttcaataa gcactattct ttcacaccac
    16981 cgatagtcaa accttttaca aagtagcgtt ggaagaatgg atacaaaatc gcgattggaa
    17041 gggttgctac cacaaccatg gccatacgac ctgtttcttt cggtagagca actcccagtt
    17101 gaccagtcaa gccgaccgct ttggcaatgt agtccatatt ttgttggatt tgcatgagca
    17161 aatattgcaa tggatacaag ttatcactct tgatgtaaag aagggcgttg aaccagtcat
    17221 tccagaaacc aagagctgtc aaaagcgtga tggttgcgat acctggtagt gacaatggca
    17281 aacagatttg gaagaaaatc cgagcctcac tggcaccatc gatacgagcc gattcgagaa
    17341 tggcttctgg aatggtcttc ttgaaaaaag aacgcatcaa gatgatattg aatggtgaga
    17401 gaagcattgg aacaatcaag gcccaaacag tgtcaccaag ctgaagtaca cgggtcacca
    17461 tgatataacc tggtaccaaa ccagcgttga acaacatact gagaaggacg aagatggtaa
    17521 aaaatctgcg atacttaaag gttgtccgtg aaatagcgta ggcataggtt gttgtgataa
    17581 agacatttgt caatgtccca actacggtta caaagacaga gatgaagagg gcttgtagga
    17641 ttttatcctt aaactgtgcc aaaaactcaa aaccgtctaa gccaaattgg gatgggaaga
    17701 agctatagcc gtattggagg aggcttttct cgtctgtcac tgaaataatg ataacgaata
    17761 caaaaggtag gatacaagag agggcaatca aacccgaaat aatactgaag aagatatctg
    17821 ctttcttact gaaggagtga atgccgacat tatcaatttt ttctttttta attttctttt
    17881 ttgccatatt ctcctccttt ctagaacaaa gctgagtttg gatcgactcg tcttgcaagc
    17941 aagtttgata ggataaccag aatcaaacca acaacggatt ggtaaagacc ggctgctgca
    18001 gccataccga tatctgctgt ctgagtcaaa ccattaaaga catatacgtc caaaacgttg
    18061 gttacattgt aaagctgacc agcattgtgt gggatttgat agaagagacc gaagtctgcg
    18121 cggaagatat ttccgactgc aaggatggtc aatacagtta caagcggagt caactgagga
    18181 atggttacgt tgcgaatacg ttgccacttg ctagctccgt ccactgtcgc tgcttcgtag
    18241 taggttggat caattcccat gatcgtcgca tagtacatga cactgctata tccaaagcct
    18301 ttccaaatac ctaggaaaag taggagatag ggccagatgc ccaggtcagc gtagaaattg
    18361 acttctttga gaccaagact ttccaataga tgattgaaca cccctttatc aatatttagg
    18421 aaggcatctg taaagaaact aatgataacc caagacaaga agtaagggaa caacatagaa
    18481 gtttgaaaaa tcttcaccat tctcttagaa cggagctcgc tgaggataat ggcaatccct
    18541 acagatacaa ctaaacctag aaagataaag ccaagattgt agaggacagt atttcgtgtg
    18601 ataataaagg cgtctcttga actaaataag aatctgaaat tatcgagtcc gacccattta
    18661 ctatttatga tactatctat gaaaccatta ctggtcatgt ggtagtcttt gaaggcaacc
    18721 acgttcccaa atactggaat gtaaaagaat agaatcaacc agagtgcccc tggcaaaacc
    18781 atcaagagaa agatccagtt gtctctcaat gtttttgaaa actttttcat aatttcctcc
    18841 ctttttattt tgatatccat ctaaaaattc ttttttagac ttttgataac gattacatta
    18901 ttagtatact cctatttgca ggttaggtta aactcctaat tatagaaaaa actccacaaa
    18961 ttatgtagca gatttaaaac tttatcacca ctatcaaaca aatgtcctaa atcaattgtt
    19021 tattttatct ctattagccc agtgatggcg tcactctgtt ataagcatcc aacaacgggg
    19081 tatactgaaa aatctccaga ctagggaact cagagatagt tcctaatctg gagattttta
    19141 atatgttatt aggcgtttgc tttcaactta gcaataacct ctttaagatt atcaatcacc
    19201 tctgcttcag tatgctcaga gcctttttca tctgccaaga acaaaactgc tttttgaagt
    19261 tctttttgag agttttcaag gacatcctta tctactgttt caaggtttga gtctttaaga
    19321 agtttactta attccttggc taatttcttg agtttgattt gcagactcat cttctcctgc
    19381 tgtttctttg cccgctgttt gtcctccatc cttagttgct gactggcttt ccttaatgga
    19441 ctctagggaa gcaatggcat ctttgactgt ttgcaagata tcacgtaaac cttgctctgt
    19501 caaactatca tctgcaaaag ctttcttagc ctctgccaaa accagacgtg ctgaatctgt
    19561 ggtaggattc gatacacctg tcaatgatct caaaagattt tctaaggttt gagtctgctt
    19621 actaatacta gactaaaatc aaaaagcatt atataacagt gatatgaaat caactaaaga
    19681 agaaatccaa actatcaaaa cacttttaaa agactctcgt acagctaaat atcataaacg
    19741 ccttcaaatc gttctatagt aaaatgaaac aaggacagtc aaatcgattt ctaacaatgt
    19801 tttagaagta gaggtgtact atttttggtt cattttacta tatcactttg aactatatcg
    19861 gtctagttat gtctaaacga ttatagctgg atactaagag aaatcaaaaa agcactaagg
    19921 gagaacgtcc caaagtgctt aatatcaagg cctcaaagcc tatcttattc aataaaatat
    19981 tacaacattt tgttgtagaa ttcaacgaca agtgcttcgt tgatttctgg gttgatttcg
    20041 tcgcgttctg gcaagcgagt caatgaacct tccaattttt cagcgtcgaa tgatacgaat
    20101 gctggacgtc caagagtagc ttctactgct tcaaggattg ctggaacttt caatgatttt
    20161 tcacgaactg agatcacttg acctggagtt acgcggtatg atgggatatc aacgcgtttc
    20221 ccgtcaacaa ggatgtgacc gtggtttacg aattgacgag cttgacgacg agtagtcgcg
    20281 agaccaagac ggtaaacaac attatccaaa cgacgttcca aaagaagcat aaagttgaaa
    20341 cctaggattc cacctttgat ttttgtagct tgtacgaaca agttacggaa ttgtttttca
    20401 cctacaccgt aagtgaaacg aagtttttgt ttttcagcca attgcaaacc gtattctgac
    20461 aatttagaac ggttgtttgg tccgtgttgt cctggtacgt agttacgacg tgccaattct
    20521 ttacctgtac ctgtaagtga aaggccaagg cgacgagctt gtttccaaga tggtcctgta
    20581 taacgtgaca tatgaatgtc ctcctgatat aaataatatt tcggtggaaa tagtcactta
    20641 gaaagccctg attcgtgcag atgcccttcg cctaaacagc caaggttact tgtcataaga
    20701 cacctgttga cgagcttcat gctttcctgc tgatatttca cacaaaatcc attttaccat
    20761 gaatagctgg atttgtaaag gggttttagg ctttattttc actaccagag aggttgagaa
    20821 cgagggtaaa ggtacttcct agaccgtact ggctgctgac tgtgatttcc ccacccaatt
    20881 gatgggccaa ttcacgcgca atcgcaagtc ctaatccatg accacctgtc ttcatgttac
    20941 gcgaagtttc gacacgataa aggcgtttga aaatattttc caaatcctct ggggcaatgc
    21001 cctgcccttc atcggtcaca ctgattgaaa gctggtcctt ctccagctta gccaccactt
    21061 ccagcttggt tcctggagca gaatatttaa aagcgttatc gaccagattc accaagatac
    21121 gagaaagctt agcataatct ccctcaatcc gggcagactc tgggattacc tgcaagtgga
    21181 catctcttct ctcctgctca atcaaaaact gaaattcact catgcactca attaagagct
    21241 tgtccagaaa aatactgtct ttactggtag tttccacctg atttctagct gtgtttaggg
    21301 tcaaaaaatt caactcctca accagtttat tgagcctctc cgtctggcgt ccaatggttg
    21361 ctagataatg agcttgctcc gactccttga taatcccatc caaaatccct tctaccgtcg
    21421 cttgaatcga agtgataggg gtcttaatat catgcgacaa ctgggcaatc atcaagccct
    21481 tttctcgttc gctttcttcc aaggaatcaa agcttacctg caaatcatgg gacatctcat
    21541 taaaagtttg ccctaattgc tgaaattcta caggaccttg aacctccaaa tttgaaggaa
    21601 aatccttggc cgctacccgc ttggcatgct ccttgagttt gcccaacgac gtaaagactg
    21661 gcaataggag aaagagacta atcccagcac cgacaaggct ggcaacgatg gtcatcccaa
    21721 gcaaaaagta aatctcgcct ttcgcaatca gcattttttg aacagcccaa aaaacaacca
    21781 aaatggttaa gagggttgaa ataatatatc caaccaaaat ataacttttt agtttcatgt
    21841 ttgtcctctc ggtttctcta tcttatatcc caacccccaa actgtcttaa tagtgggcgt
    21901 ttggtcacta ctatattttg ccagctcctg tcgaagagca tggatatgca cattcaaggt
    21961 attggtgtca tccacgtagt cttctttcca gatcttttca tagaggtctg tcttggagaa
    22021 aactcgctct ggattgctag ctaaaatcca cagcaattca aatgatttaa cagttaaatc
    22081 cagcatttct tctcctattt gaacttcatg actactatga ttcattttta gattgccaag
    22141 ggaaatcagc tctgtttcgc ccccacgatg aaggcggcgc aaaatattgt ggacacgcaa
    22201 aactagctca cgtgggctaa aaggcttagc aataaaatca tctgctccca agctcaggcc
    22261 gtaaatcttg tcctgttcac tggtcttagc agtaataaat aggaaaggct gctctggtga
    22321 taagtattga acctcactga ttaaatcata accatccatc cgaggcatca tgacatctgt
    22381 gataatcaaa tcaatcggtt ttttcttgaa cagctctagc gcttccagtc catcatgggc
    22441 taccaagacc tgataacctg cctgaattaa gtatctctga tgaatatctg tgatttctac
    22501 ctcgtcgtca acgagtaaaa ttgtctttcc catctgtctc tcctttgata aaaacagtgc
    22561 tatactgctt taagtataac actattttta ttgttttacg atgattttaa tctgctattg
    22621 aaagaaagtc ctgattgctg attgagttgt taaactaatc ttagtcagcc cactatccat
    22681 catttatttc cttagctgat tagttttctt cttttttgtt tttcaaacct agaccaccga
    22741 gtaaacctgc aagcgcaagc ccaaggaaac caatacttgc cattgatgtt tgagtctcac
    22801 cagtatttgg tagcatagct ttatcctctg acatcatcgt atcagacatc ttgttagcag
    22861 aagcagccat gttttcacct gccatcgtgt tggtagaact tgtcatggtg tcagcaggca
    22921 tgctatctgt agtacctgta gcatgattgt gattcatcgg agtcacgcca gaaccagagt
    22981 tagaaggtga taatgaacca tttgctgtgt ctgatgtttc tttaacattt at
//
LOCUS       DAVLVZ010000026        22253 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000026 DAVLVZ010000000
VERSION     DAVLVZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22253
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.026"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..578)
                     /locus_tag="VYN77_001062"
     CDS             complement(<1..578)
                     /locus_tag="VYN77_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558534.1"
                     /translation="METLQTIETKIDKLIEQNKKAIEMTEAELVKASQAISDAQAKLV
                     QAQEEINSEKYVEAKSDLWTAERTKEFHEGRLKELVTTPIISYDEYHATVKEIYRLAD
                     EQQNTFFTPAVAKLLEIVKLGDESAKEVGKVNEIFRKLEKDISKNNEDYKRDKNGGWF
                     RGPFSSLSYSPRNALHGYQDDLKEIAESFKKG"
     gene            complement(732..896)
                     /locus_tag="VYN77_001063"
     CDS             complement(732..896)
                     /locus_tag="VYN77_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEV1558535.1"
                     /translation="MNPNKDYTPFLQDNFIIFNKNGIIEMKKIPDFGSVVFTSQDGNI
                     VQIETTIKER"
     gene            complement(1435..2886)
                     /locus_tag="VYN77_001064"
     CDS             complement(1435..2886)
                     /locus_tag="VYN77_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HEV1558536.1"
                     /translation="MNSNDIVNKIIEENQQEAPPEVVDLTQARKTNEEHNSLNLAKRP
                     RGDGFDRGLDNLRKILSGDSKLKGAIQYNTFTYEIEVTRPMKLNGRTLNGAIDDLIIR
                     EIRAYIATKYKLDYKKPDIADFLEIVAGENSYNPLKDYLESCESEYKELVNQREPFDI
                     LRHYLNIKDDEYNRIIMDLFFRGAVAKVFDPTVKFDFVLDLTGRQGVGKTQFFEGLFT
                     HKYFTTVETFTDKDDKARMVRNWCVFDDEMVASKKASFSELKKFITETKLEFRPPYAS
                     SDRRLPKSFIIVRATNDHDYLNDLTGERRFLVVEVHKDTAYKGRKWTEKDRRAFWGAM
                     VVAWRANQVLNLTDEQEKLVNEVRSRYKFVDEILEDLERYLDTPYPKRMYQFPMTDRM
                     RYYYIYDMMNEGYYRNNRGGTVELDTDTYGELVDRDKMTINLFFQEVYLTDKAPPKDK
                     AKVKKYMQNRDGWEHRRSTRFGKSIKPAYVKKM"
     gene            complement(2879..3394)
                     /locus_tag="VYN77_001065"
     CDS             complement(2879..3394)
                     /locus_tag="VYN77_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558537.1"
                     /translation="MRVIELILSADKLPLFGFLKSTPTQVWKNGEHYKFIYFEPIGEG
                     LTAFHYKGLYVAVKDENEEVEGWELTRDLEIGLASPDLLMILKNLEVNKLTEQRQGLG
                     VELKGWVFNLICNGIYTRYETSLFVRLLFVNGYSFSQLVDLFSAIVKRKDLASYFLEV
                     ATKFYKEVAFE"
     gene            complement(3391..3663)
                     /locus_tag="VYN77_001066"
     CDS             complement(3391..3663)
                     /locus_tag="VYN77_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558538.1"
                     /translation="MFSLSRESEKDLTHGILEVVERYLEAHEKVPPRLTKLINRIELK
                     EELKISDNTLNKWESQGLRRYQPPDDGSRMIYYLVTDIWKFLGVDE"
     gene            complement(3656..3997)
                     /locus_tag="VYN77_001067"
     CDS             complement(3656..3997)
                     /locus_tag="VYN77_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1558539.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGLEISLTTKLTERTVQDIIKR
                     LIIKHNIPIVGVRNGFYRGYFIPRNKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            complement(3978..4271)
                     /locus_tag="VYN77_001068"
     CDS             complement(3978..4271)
                     /locus_tag="VYN77_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558540.1"
                     /translation="MSYTVKIYLDFEKIPDETYFTKEIFLAMCTDNATKLAVTMGLLA
                     KQALDLETRAVILKTLNTIIENQKAILQGVASGQITFLNKSKGEEELDESIRE"
     gene            complement(4268..4483)
                     /locus_tag="VYN77_001069"
     CDS             complement(4268..4483)
                     /locus_tag="VYN77_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558541.1"
                     /translation="MNELDLSNTQAVFVSVVLIGILLYLNHRDRKKSAQIERENQQTI
                     ETPSDDLNPDYGRYIQLAGVRVSGGME"
     gene            complement(4485..4664)
                     /locus_tag="VYN77_001070"
     CDS             complement(4485..4664)
                     /locus_tag="VYN77_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558542.1"
                     /translation="MTQSEFYNRMTKIKDRHPNLFQFIIDFLDDKVSTEEVYDFLKME
                     RSYQVNHIKNYKARA"
     gene            complement(4661..5113)
                     /locus_tag="VYN77_001071"
     CDS             complement(4661..5113)
                     /locus_tag="VYN77_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558543.1"
                     /translation="MTETTYDIIAKSLDRISMELHQADENNDFLRIGLLSGQLKAIKE
                     NLHRLLWIELPELNESHKIEAVSKSTTGIFFHPGIFEMDAMRQAFFKRQAKHFFDNAT
                     EQQEYIEYAEKEYLEATKTLKDILFNTNNGTRQVNKDCLVEKFEEAMQ"
     gene            complement(5460..5888)
                     /locus_tag="VYN77_001072"
     CDS             complement(5460..5888)
                     /locus_tag="VYN77_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558544.1"
                     /translation="MGKKHQVVKFKDIAEKLPELEGKNLEEIAGVLGYRNLESCRVNL
                     YNLRQNKRLGFEVEKGVYTKFELLDDTVKEELEEKELSERGHFLQSLDYYKVAKNAFE
                     IAEDKTVKAETRQKAERDILDAMKHIPKKHRAILYDMMEG"
     gene            complement(6073..6273)
                     /locus_tag="VYN77_001073"
     CDS             complement(6073..6273)
                     /locus_tag="VYN77_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1558545.1"
                     /translation="MSKLRGYRVMLGLTQQQMADKLKISLQSYNNKELGKTPFNDKER
                     LAIKSMVAEIKPDITIDEIFYS"
     gene            6493..7089
                     /locus_tag="VYN77_001074"
     CDS             6493..7089
                     /locus_tag="VYN77_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1558546.1"
                     /translation="MNRLKELRQEKKLSQKEIAETLGFSLRSFQRMENGESQIKPEKA
                     QLLADYFGVSVANLLGYENNFIESVKNLSQKDGSDEVFFKAFRAYYELKTADGRENLL
                     TLKDEDFLNKYREEILKNLIPNIKELSKQEIEKYLSDEKLLNEAKQKLNDFLFTIGTL
                     NPQETQLLVDFISLSYKDKQIVLNILGSLNQKETHHDL"
     gene            7079..8005
                     /locus_tag="VYN77_001075"
     CDS             7079..8005
                     /locus_tag="VYN77_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEV1558547.1"
                     /translation="MTYSFIALDVETANSFRGSLCSIGLVKFIDGQEVDSFYTLINPE
                     EKFSSRNIKIHAIKPEDVIGAPTFPEVQKEIINFIDNLPIVAHNARFDAYALQDVYLK
                     YEIPFDNIQYFCSYQVCKIILTDLPNHKLHTLAEHFKISLDHHNALSDARACGLILLE
                     ILKLSKQTSIRKMLKNLGYPELGLIGKHGFVKNKSTYIADSGVSNLKNDDKKDNKNNI
                     SNNNEIPQTKIFDAKTKAKNIKFHYVNKWIYIILAIVLGWIGGHHFYAGYNRKGFLYL
                     LFSFTFIPMLLALFQVISALLKTPDSNGKILV"
     gene            8025..8255
                     /locus_tag="VYN77_001076"
     CDS             8025..8255
                     /locus_tag="VYN77_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558548.1"
                     /translation="MDYAQDYLDDILVRMAYHSSGIEGNTISLPETVSIILEKSIIVM
                     FNIANTSQRINVNAYTYFQNKNKKVQKKRQKS"
     gene            9147..10313
                     /locus_tag="VYN77_001077"
     CDS             9147..10313
                     /locus_tag="VYN77_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1558549.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKDGSTVYKTVEIKTYAELVENWLETYCHTVKKATLTIIKSRLNNYLL
                     PAFGEYKLDKLTPPVIQKQVNQWAKEYNQLGKGYQEYPQLNSLNKRILKYAVSLQVIP
                     FNPARDIIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLQNGTLDVNKTLNCEKEVTTPKTKSSIRVIDLDNKTVLMLRLY
                     KNRQAQVGREIGLTYEKVFSNSFDEYRDARALRSRLEKHLKLSDCPRLTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHSQISITMDTYSHLSKDNKKNATSFYEKAIEKLKSS"
     gene            10452..11024
                     /locus_tag="VYN77_001078"
     CDS             10452..11024
                     /locus_tag="VYN77_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558550.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(12069..14327)
                     /gene="glgP"
                     /locus_tag="VYN77_001079"
     CDS             complement(12069..14327)
                     /gene="glgP"
                     /locus_tag="VYN77_001079"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV1558551.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLSLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(14353..15870)
                     /gene="malQ"
                     /locus_tag="VYN77_001080"
     CDS             complement(14353..15870)
                     /gene="malQ"
                     /locus_tag="VYN77_001080"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1558552.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEESLLDLTTIYRRINENLVDLKK"
     gene            16421..17692
                     /locus_tag="VYN77_001081"
     CDS             16421..17692
                     /locus_tag="VYN77_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1558553.1"
                     /translation="MSSKFMKSAAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            17803..19095
                     /locus_tag="VYN77_001082"
     CDS             17803..19095
                     /locus_tag="VYN77_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1558554.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            19097..19939
                     /locus_tag="VYN77_001083"
     CDS             19097..19939
                     /locus_tag="VYN77_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1558555.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            20192..20992
                     /locus_tag="VYN77_001084"
     CDS             20192..20992
                     /locus_tag="VYN77_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1558556.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQTPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            21002..21988
                     /locus_tag="VYN77_001085"
     CDS             21002..21988
                     /locus_tag="VYN77_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1558557.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            22055..>22253
                     /locus_tag="VYN77_001086"
     CDS             22055..>22253
                     /locus_tag="VYN77_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558558.1"
                     /translation="MRFIVGRFTSFSTEIESFPKIFLYSMKIKVQTRKLAAGCSKHCF
                     EVVDRTDEVSSKHGFEVVDETD"
BASE COUNT         6658 a         4623 c         3884 g         7088 t
ORIGIN      
        1 tgacccttct taaaactctc tgctatttct tttaaatcat cttgataccc atgtaaggcg
       61 tttcttggag aataggataa acttgaaaat ggcccacgaa accatccacc gtttttgtcc
      121 cttttataat cttcgttatt tttagagata tctttttcaa gttttctaaa tatctcgttt
      181 actttaccaa cttctttggc ggactcatca cccagcttta caatttccaa tagtttggct
      241 actgctggag taaagaaagt attttgttgt tcatcagcca atctataaat ctctttaacc
      301 gttgcgtgat attcatcata tgaaattatt ggagttgtaa ctaattcttt caaacgtccc
      361 tcatgaaatt cctttgtacg ttctgccgtc cataggtcac tttttgcctc gacgtatttt
      421 tcagagttta tttcttcttg agcctgtact agcttggctt gggcgtctga aatagcttgg
      481 cttgctttaa ccagttccgc ctcagtcatc tcaatagctt tcttattctg ttcaatcagt
      541 ttgtcaattt tagtttcaat agtttgtaat gtttccatgt tcttttattc ctcatttctt
      601 ttgattgtgc ttattggttt caactcttta ctgggtcttt attcctcatt acttcctctt
      661 tctcaaaaca aaaagagacg caacaaaaaa gctacttagc tttaatgtta cgcctctagt
      721 tgtctagtca gctatctttc tttaattgtt gtttcaatct ggacaatgtt gccatcttga
      781 ctagtaaata ccacgctacc aaagtctggt attttcttca tctctattat accatttttg
      841 ttaaagataa taaagttatc ctgtagaaat ggagtgtaat ctttgtttgg attcatctgt
      901 ttttcctctc ttttttggtg ccatatattt aacatacctt ctattctgtt aaatttaagc
      961 gacctccctt ctcgccttgt ctatcaaggg aggagggcta tttttgtgat ttcctattta
     1021 cattttctca ttatgtaaaa tacaaatatg atttcagtct taaaagttac tcatttaagt
     1081 tactatttcc atttcttttt aggtctcctc ctcacaattc caaaaagtgt agtcatttta
     1141 ggatatctag tgactacgct ctccgcctta cagtcacaag gcttttcgcc atgcgtagtc
     1201 atgtagtcac cttgctccca aaaaaataaa caataaacgc cttttatctc ttatcttcat
     1261 atactcttta ataaatagaa aataactaca tttttatata aaagtcaata ataccaaggg
     1321 tttaggggtg tagtcagtaa aatagtaaaa acaacacttt tttctaaccc cttatgtatc
     1381 aagggttttc ttcctgttta aaatgtagtc acttttaaaa ataaactaca ctgactacat
     1441 ttttttaacg taagctggtt taatactttt accaaatctt gttgatcgcc tatgttccca
     1501 gccgtctcgg ttttgcatat actttttaac cttagcctta tcctttggcg gtgctttgtc
     1561 agtcaaatac acctcttgaa aaaataggtt aatggtcatc ttgtccctat ctaccagttc
     1621 gccataggtg tctgtgtcaa gttcaactgt tccacccctg ttatttctat agtatccctc
     1681 gttcatcata tcatagatat aatagtagcg cattctgtcg gtcatcggga actgatacat
     1741 tctttttggg taaggagtat ctaaatagcg ctccaaatcc tcaagaattt catctacaaa
     1801 cttgtaacga cttctaacct catttactag cttttcctgc tcgtccgtca ggttcaagac
     1861 ttggttagcc ctccaagcta ctaccatagc gccccaaaag gctctgcggt ctttctctgt
     1921 ccatttcctg cccttatagg cggtgtcctt gtggacttct acaactagaa agcgcctttc
     1981 ccctgtcagg tcgttcaaat aatcatggtc attagttgct ctgacaatga taaaactctt
     2041 aggcaatcgc ctgtcactgg aagcgtaagg gggtctaaac tctagcttgg tttctgtgat
     2101 gaatttcttc aattctgaaa aactagcctt tttactggcc accatctcat catcaaagac
     2161 acaccagttt ctcaccattc tagccttgtc gtctttgtct gtgaatgtct caacggttgt
     2221 aaaatacttg tgagtaaaaa gcccctcaaa aaattgtgtc tttcctactc cctgccttcc
     2281 agtcaagtcc agcacaaagt caaacttaac ggtagggtca aacaccttgg caaccgctcc
     2341 acggaaaaac aaatccatga taatacggtt atattcgtca tccttgatat taagataatg
     2401 ccttaaaata tcaaagggct cacgttgatt cactaactct ttatactcgc tttcgcaaga
     2461 ttccagatag tcttttaagg ggttgtagct attttctcca gccactatct ccaaaaaatc
     2521 agctatatca ggctttttat agtctagctt atacttggta gcaatataag ccctaatctc
     2581 tctgataatc aggtcatcga ttgcaccgtt cagggttcta ccgtttagct tcattggtct
     2641 agtcacttca atttcatatg taaaagtgtt gtactgtatc gcccctttta gcttgctatc
     2701 tccgctcaaa atctttctca gattgtctaa acctctatca aagccgtccc ctcttggtct
     2761 ctttgccaag ttcaggctat tgtgttcctc gttggttttc cttgcttggg tcaagtccac
     2821 cacttcaggc ggtgcttctt gctgattctc ttcaatgatt ttatttacaa tgtcattgct
     2881 attcaaaagc cacctcctta tagaattttg tcgctacttc tagaaagtag cttgctaggt
     2941 ctttgcgttt gacgattgct gaaaacaagt ccaccagctg actaaaactg tagccattga
     3001 caaatagcaa gcggacaaaa agcgaagtct catatctggt ataaatgccg ttacaaatca
     3061 ggttaaaaac ccagcctttt aactccactc caagcccctg ccgttgctct gtcaatttat
     3121 ttacctctaa atttttcagg atcatcagca agtcaggact agccaaacct atttctaaat
     3181 ctctggttag ttcccagccc tctacttcct cgttttcgtc tttaactgcc acatacaaac
     3241 ctttgtagtg gaaagccgtc aagccctcgc ctattggctc aaaatagata aatttgtagt
     3301 gttccccatt cttccaaact tgggttggcg tagacttgag aaagccaaac aaaggcaatt
     3361 tgtcagcgga taaaattaac tctatcaccc tcattcatcc acccccagaa acttccagat
     3421 gtcagttact aaatagtaaa tcattcttga accatcgtcc ggcggttgat agcgtcttaa
     3481 accctgactt tcccacttat tcagcgtgtt gtcactaatt ttcaattcct ctttgagttc
     3541 aatcctattt attaacttgg tcaatcttgg cggtactttc tcatgcgctt ccaggtatct
     3601 ttccactacc tccagaatgc catgcgttag gtctttctcg ctttctcggc ttaggctaaa
     3661 catatccgcc cacctccttc aaggtttctt tgtagctttc taaatcgcta ttcatcaaca
     3721 ccgctaggcg cttgctctcc tcctgtactt ggttgtaaaa agccttagca ccatctagta
     3781 actcgccttt gtttctaggt atgaaataac ctctataaaa tccatttcta acccccacaa
     3841 ttgggatgtt atgcttgatt attaaacgct taataatatc ttgcacggtt ctttctgtta
     3901 gcttggttgt caggctgatt tctaaccctg ttatggagtt ctcagcccct accttgatta
     3961 gtcttaaaac tcgcttgtca ttctctgata gactcatcca gttcctcctc tcctttactt
     4021 ttattcaaaa aggttatttg tccgctcgct accccttgca aaatagcttt ttgattttct
     4081 atgattgtat taagggtttt taaaataaca gccctagtct ctaaatccaa cgcttgtttt
     4141 gccagcaaac ccatagttac agctaacttt gttgcattgt ccgtacacat agctagaaaa
     4201 atctctttag taaaataggt ttcatctggt attttttcaa aatcaaggta tatcttcact
     4261 gtgtaactca ttccatccct ccggaaactc ttacccctgc aagctggata tatcgcccat
     4321 aatcagggtt taaatcatcg ctaggtgttt ctatcgtctg ttggttttct cgctcaattt
     4381 gggcgctttt tttgcggtct cggtggttta gataaagcag tatgccaatc agtaccacgc
     4441 taacaaaaac cgcctgtgtg ttgcttaaat ctagttcgtt catgttatgc tctcgctttg
     4501 taattcttga tatgatttac ttgatagctt cgctccatct tcagaaaatc atacacctct
     4561 tcggtgctta ctttatcatc taaaaagtca atgatgaact gaaagaggtt cggatgtcta
     4621 tccttgattt tagtcattcg gttgtaaaat tctgattgtg tcattgcatt gcctcttcaa
     4681 atttttctac aagacaatct ttatttactt gtctagtccc attattagtg ttaaaaagga
     4741 tatccttcaa ggttttagta gcctctaaat actccttttc agcatattct atatactcct
     4801 gttgctctgt tgcattgtca aaaaaatgct tggcttgacg tttaaagaat gcttgtctca
     4861 ttgcatccat ctcaaaaata cctggatgga aaaagattcc agtagtactt ttagagactg
     4921 cctcgatttt atgactttca ttcaattcag gaagctcaat ccaaagtaag cggtgtaaat
     4981 tttctttgat agcttttaat tgtcctgata agagtcctat tctcaaaaaa tcattgtttt
     5041 cgtctgcttg gtgtaattcc atactaattc tatccaagct tttagcgata atatcgtatg
     5101 ttgtttctgt catagtctgt tttccctttt tcctatattg gattatttca ccactccaaa
     5161 cgctgggcat ttgccccaag ttggcggacg cttgtagtga tgtttcgtgg gtaatcaccc
     5221 acattttttc taaacaagtg cttagaatcg tcgtgtcagc actcgatttc aaaacctttt
     5281 ctaattgctt gcctgctctt cggttttctt agtttttctt cgtttcgttt tcgatttaat
     5341 cgcctaatct tttccaggct ctatcaaatt catcacgttg aatttcttta ctatgtaatt
     5401 gccttgctaa ctccatgcct ctacgcatga atctagcgaa gtgggttttt gcttccatcc
     5461 tagccctcca tcatgtcata aagtatagcg cgatgttttt taggaatgtg cttcatcgcg
     5521 tccaatatgt cacgctctgc tttttgtcgg gtttctgctt ttactgtttt atcttctgct
     5581 atttcaaagg cgttttttgc tactttgtag tagtctagcg actgtaaaaa atgaccacgt
     5641 tcagacaatt ctttttcttc cagttcttct tttacagtat cgtccaagag ttcgaactta
     5701 gtgtaaaccc ctttttctac ttcaaaccct agacgcttgt tttgtctgag attgtaaagg
     5761 ttgaccctgc aactctccaa gttgcggtag cctaatactc cagcgatttc ttctaaattt
     5821 ttaccttcta attcaggcaa tttttcagct atatctttaa attttactac ttgatgtttc
     5881 tttcccattg tttaccttgt ctttctatgc tataatcaag gtatagaaaa aatatctata
     5941 ctcttgatcc agtcgcttgc ttctgtcgcc aaacattcag caagtgactt ttttgttgtc
     6001 aatccccagt ggtaaagcac cacatgagaa atctgtaaat atagagtagt attgcgatta
     6061 gttcgctcct ttctaactat aaaatatttc gtctattgtt atgtctggtt tgatttctgc
     6121 aaccattgac ttaatcgcta gacgttcttt gtcattgaat ggcgttttac ctaattcttt
     6181 gttgttgtac gactgcaaag aaatttttag cttgtccgcc atttgttgct gagttagtcc
     6241 taacataacc cgatagcctc gtagtttgct catagatttg ctcctttcta aaaaaatccc
     6301 cctccatgaa tagattgaaa gtgtgaaagg ttggaggggg tgcttgacgt cattttatga
     6361 cgcaacataa gcttgatttt tattataacg tcagttttag acgttgtcaa tagttttttt
     6421 gattttttta cgtcattttt tgactttttt taaacataag gttataatca tctgtgaaag
     6481 gtgtgataaa acatgaatag attgaaagaa ttaagacaag aaaaaaagct atctcaaaaa
     6541 gaaatagctg aaactttggg attttcttta aggtcattcc aacgaatgga aaacggagaa
     6601 agccaaatta aaccagaaaa agcccaacta cttgctgatt acttcggggt aagcgttgct
     6661 aacttactcg gttatgaaaa caattttatt gaatctgtaa aaaatttaag tcaaaaagat
     6721 ggctctgatg aagtattttt taaagcattt agagcatact atgaactaaa aacagctgat
     6781 ggtagagaga atttattgac tttaaaagat gaagattttc taaataaata tcgtgaagaa
     6841 attttgaaaa atctcattcc aaacataaaa gaattaagta aacaagaaat tgaaaaatat
     6901 ttatcagatg aaaaactttt aaatgaagca aagcaaaaac ttaatgattt tctatttacc
     6961 attggaacat taaatcctca agaaacccaa ttactagtag atttcatatc cctatcttac
     7021 aaagataagc aaatagtatt aaatatactt ggctcactaa atcaaaagga aactcaccat
     7081 gacttatagt tttatagctt tagatgttga aacagcaaac tcatttcgcg gaagtctatg
     7141 ttcaattgga ctagtaaaat ttatagatgg acaggaagtt gactcctttt atactttaat
     7201 taatcctgaa gaaaaattta gtagtagaaa tataaaaata catgctataa aacctgaaga
     7261 tgtcattgga gcccctactt tccctgaagt acaaaaagaa atcattaact tcattgacaa
     7321 tctacctatt gttgctcata atgcaagatt tgatgcttat gctttacaag atgtttattt
     7381 aaagtatgag attccttttg acaacattca atatttttgt agctatcaag tatgtaaaat
     7441 aatcctaact gatttaccaa atcataaatt acatacctta gctgaacact tcaaaatatc
     7501 tctagatcat cacaatgctt tatcagatgc aagggcttgt ggtttaatat tattagagat
     7561 attgaaatta tctaaacaaa caagtataag aaaaatgctt aaaaatttag gatatcctga
     7621 actgggatta attggaaagc atggatttgt aaaaaataag agtacatata ttgccgactc
     7681 tggagtttca aacttaaaaa atgacgataa aaaagataat aaaaataata ttagcaataa
     7741 taacgaaata ccacaaacaa aaatttttga tgccaaaacc aaagctaaaa atattaaatt
     7801 tcattatgtc aataagtgga tttacattat attagctatc gttttaggtt ggataggtgg
     7861 tcatcatttt tatgcaggat ataatagaaa aggattttta tatctactat tcagtttcac
     7921 ttttattcca atgcttcttg cacttttcca agtaatttca gcattgttaa aaactcctga
     7981 ttccaatggg aaaattttag tttaatttta cagaaaggag aatcatggac tacgctcaag
     8041 actacctgga cgatatactg gtacgcatgg cctaccattc tagcggtatt gaagggaaca
     8101 ctatttccct acccgaaacc gtcagcatta tcttagaaaa atccataatc gtaatgttca
     8161 atatcgccaa tacttcccag cgaataaatg tcaatgccta cacttatttt caaaataaaa
     8221 ataaaaaagt acaaaaaaag cgacaaaaaa gttgattttt aataaaaaaa cgatagagtt
     8281 atattagaaa gtcgcttgta attctgatat gtctgcgtat ttttcgtaag ttgcctattg
     8341 ggtaacctgc tatcagttaa gggcttttat attttacaaa aaaatcagtg tatcggctta
     8401 ttatgttggt acacttttct ttttactaca ctagttttct acaacaaaaa aaaagacaag
     8461 ctagctgctg ctgacttgtc taatatcgcc tgaactgaaa taacgggaaa gattcaggcg
     8521 gttacatgac tagtttacca tattatccag aaaaacaaaa aaggtaagtc aataccctgt
     8581 catcaggcta taactctcta atgaagtata atgcggtata atttagacaa ttttcaacac
     8641 tttggcattt tagaaacgct taaagcgctc taaacaatat caaaaaaaat aaaactcttg
     8701 aaatattggg gcttgtatgc tagaatgaaa gcataaaggg aaaaacagtg cttctagcat
     8761 atgtaactaa aaagccccac cgttacagcg atggggtttt ttgctaccct cttaaagggt
     8821 taaatgctac ttgctacgtc tctttatcca tagcttgaaa agctcagata agacgttaac
     8881 aagtaacgga gctagaaaaa gggaaaacaa ctctagcata tataacacct cccttctcta
     8941 aattgagggg gcgaaaacat tatatcaaaa acacccatca caaacaagcc catataagtc
     9001 ccatatctgc cttattccct tttctggtac aatttaccgt ctgactaatt aaaatcgaaa
     9061 ataggggtat tctcgtagcc cctcgcatgg tataaactca aaatcttttc taattgcttg
     9121 cctgctgatg gaagaggagt aaaaccatga aaataacaga atacacaaaa aaagacgggt
     9181 ctacagtcta tcgctctagc gtctatcttg gtatcgatac cgtaacaggt aagaaagtca
     9241 agacgactat atcagggcga accaagagag aactcaaagc caaagcgcta caggctcaaa
     9301 tagactttga aaaagacggg tctacagtat ataagacggt ggagataaaa acttatgccg
     9361 aactggtgga gaattggctg gaaacatact gccatacagt caaaaaggca acacttacaa
     9421 taatcaagag tagactaaat aactatctac ttccagcctt tggagaatac aaactagata
     9481 agctaacacc cccagttatt caaaagcaag ttaatcaatg ggcaaaagag tacaatcaac
     9541 taggcaaagg ttaccaagag tatccgcaac ttaactcgct aaataaacga atacttaaat
     9601 atgccgtatc cttgcaagtt atccccttca acccagcccg tgatattatc gtgccacgtc
     9661 gtaaggaaaa agaagggcaa aaactgaaat atctggatga tgataactta aaaaaattct
     9721 tgacttacct ggagcagctg ccaaacactt acaaaaattt ctacgatacg gtgctatata
     9781 agacgctttt agcgactggt ttgcgtattc gtgagtgttt agccctcaaa tggtctgata
     9841 ttgacttaca aaacggtaca ctagacgtaa ataagactct aaactgtgaa aaagaagtca
     9901 ctacccctaa aactaaatca agtattaggg taattgactt ggataataag acggtgctca
     9961 tgctgcgact ttacaaaaat agacaagcgc aagttggtag agaaataggc ttgacttatg
    10021 aaaaagtatt ttctaattcc tttgacgaat atagagacgc tagggcgctt aggtcaagac
    10081 ttgagaaaca tttaaaactt tctgactgcc ctcgtttaac gttccacgct ttccgccaca
    10141 ctcacgctag tatcttgctc aatgccggac tgccttacaa agaaatacaa acaaggctag
    10201 ggcattctca aatatcaata accatggata catatagcca cttgtccaaa gacaacaaga
    10261 aaaacgctac ttcattttat gaaaaagcca ttgaaaaatt aaaaagttcc taaaaagtcc
    10321 acaaatttta taaatcggtt atgaatcaag ccacaaatac cgctatacta gggaatttac
    10381 cccctatact tgaaataaag cgcatttcaa aataaaatgt aagcagatac aaataaaaag
    10441 gagaaaagct catgaacaaa ttaatgaaat ttatttcggt ttttttgacg tcaattgtgt
    10501 taattgtatc agcgattcca agtgtttcag ctgtatacgc ttctgaacaa gtatcgcaaa
    10561 ttgaaacaaa tatggaactt caacctgtca cttctctaac agaagaacaa atcaatacac
    10621 ttgcaaacga aatccaatct tttcatccag acgtctcaca acaatggatc aaagaagtaa
    10681 ttaaccgaca attacaaggc gattatacaa tcccacctac atactctcca tttagagcag
    10741 cttggcaagg tattacagtt aatcaaatgg gtgctctatt agatactgca atagctttag
    10801 cattaggagg aactactgca ggccttgcaa atctaattaa agtaaaagga aaacatgcag
    10861 caaaaagtgc tattcgttca gcaatttcta gatatctagg tagttggttt gtaaatgatg
    10921 ttgctttaga atccgctatg aatttattat caccggggac ttatttagca caattatggg
    10981 ataaaaatga tgccattcct aacaacggaa ggattaactt ttaatcatga gatatacaat
    11041 aaaattaatt gggtataacg tattttcgtt gtttatattc tttatcttat caatgttgtt
    11101 taaagttccc tttaggtggt ggatcagcat tgcaatgacg acttcgtcgt acattgtata
    11161 taattactta aagaaaaaga gagaaaagga atgatcttat accagatcat tccttttctg
    11221 ctttctgtaa atccctcgga gtgtgtttgg cgctttgata cgaagtgtca aagctgtata
    11281 aacgttacta tttccaaaaa agtaacagaa aattcctgct ttgtaggact gctttgggtg
    11341 agtaatagac atgtcgactc accctatctc ctgatggcga aaagaattta gcaatggtgc
    11401 aagttgttct gaactcgaga aaaaacatgc cgaaatcgaa gaaaagcgtg cagaacttga
    11461 agcgaagaag caagaaatgt tgaatcgctt ggataaatag aatttagaaa acagaaaaaa
    11521 ctaaaaagaa tgtagggttt atttccctac attcttaaaa taacttagta cataaatgac
    11581 aatgctgcca ctgccgtcaa tattgcaaaa actattttcc aaattttttt attttttcca
    11641 aaaatcttat caggtaacaa gcttattaca agaaaggtaa taaacaacac caatttaagt
    11701 gtgtatatca taaaaacacc ttctttccta caattatatt ataatacttt atgtaagcga
    11761 acacaagaga tgtgaggtaa ccaactacga aaaaatataa ggctgaagac agtaaaaacc
    11821 agcatttaaa gccttcactg tatctaggat ttccctattt ttagacaaga ttgacccaaa
    11881 tccacagcat ttaaaaaagc gaatttcaat cgaaactcgc ttttttacaa gatgtattag
    11941 tataaatttg tatcttatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag
    12001 gttgctcaaa gcaccgcttt gaggttgcag ataaagctga cgtggtttga agagattttc
    12061 gaagagtatt agttcaagtg ccagatgtct tcgttatact gagcgattgt acggtcagat
    12121 gagaagaatc ctgctttaga aatgttaacg atgactttat ccaaccatgc gtcacggtct
    12181 tcgtagtcag caagcatttg ctctttgact ttgatgtagt cttccaaatc aagaagagtc
    12241 atgaaccagt ctttgttgat caattcattg taaagacgtt ccaagcgctc tttgtttcca
    12301 gctgcaagaa ctgcatcact aacgatgaag tcaaccaatg gtttgatagc ttcacgagcg
    12361 tagaattcgc ttgatttgta agctgctttt gcgtaaaggt cgataacagt ttctgaatct
    12421 tcaccgaaga tgtagatgtt ttcttctcca accaactcag cgatttccac gttagcaccg
    12481 tccatagtac caagtgtcaa agctccgttc aacatgaatt tcatgttacc agtacctgaa
    12541 gcttctttag aagcaagtga gatttgttct gagatatcac atgctgggat aaggaagctt
    12601 gctgcagtaa cgttgtagtt ttcaaccata actacttgca agtgtggagc tactgctgga
    12661 tcgttagcaa taacttctga catgcaaagg atcaagtgaa tgatatcttg agcgattgtg
    12721 taggctggag ctgctttacc accaaagaag attgtgattg gacgagcagg gatgttacca
    12781 gctttgatgt caaggtattt gtggattacg tacaaagcgt tcatttgttg gcgtttgtac
    12841 tcgtgaagac gtttgatttg gatatcaaag atagaatttg aattgatttc cacaccttgg
    12901 tgttctttca agtgacgagc caatttacgt ttgttgtgag ccttgatgct ttccaatttt
    12961 tctttgacag ctgctttgtc ttcataagac aaaagttttt caagctcatc tgcttcatgg
    13021 tgccaaccat ctccaagaat ctcatccaag tagtgagaca atcttgggtt agcatgcata
    13081 agccaacgac ggaaagtgat accgtttgtt ttgttgttga acttttctgg gtaaaggtcg
    13141 tagaaggctt tcaactcaga atttttcaag atttcagtat ggagtgctgc aaccccgtta
    13201 acactgtatc cgtagtggat atccatgtga gccatgtgaa cacgtccgct ctcatcgatg
    13261 atttgaacag ctggatcttt gtactctgcc ttcacacgac ggtccaattc ttcgatgatt
    13321 ggtaccaagt gaggaaccac ttcttgcaag aattcaagag gccatttttc aagcgcctca
    13381 gcaaggattg tgtggttagt gtaggcagtc atgctacgaa caattgagat tgcttcgtca
    13441 agctcgatac cacgtgcagt caaaagacga atcaattcag gaatcaccat tgatgggtga
    13501 gtatcgttga tttggacaac tgcgtagtca gcaaggtcat gcaagttgct tcctttttcg
    13561 attgcttcgt cgatgatcaa ttgcgcaccg tttgaaacca tgaagtattg ttggaagata
    13621 cggagcaatt caccttgacg gtcactatca tctgggtaaa ggaagagagt taagttgcga
    13681 gcgatatctg tcttgtcaaa gttgatacca tctttaataa tagaagaatc aactgaatcc
    13741 aagtcaaaca aacgcaagcg gtttttagtc gctgtttcat aaccagtaac atcaatatcg
    13801 taaagagttg atgtcaaagt aaagtctgca aatggtactt ggtagctacg gcttgagcga
    13861 accaaccagt tttgctctgt caaccatgca tttggaattg tttcttgttg gttgttttta
    13921 agaacttgtt ggaaaagacc aaagtggtag ttaagaccaa caccgtcacc attcaaacta
    13981 agagtagcaa ttgagtcgat aaagcaggca gccaaacgtc ccaaaccacc attaccaaga
    14041 gatggttcca attcaacttc ttcaacttcg atcaagtctt tacctgcagc tgcaagttct
    14101 tttttaacat cgtcgtaaag accaaggtta atcaagttgt ttgacaagag tttaccaatc
    14161 aagaactcag ctgagatgta gtaaactttt ttcttacccg tgttgactgg tttttggctg
    14221 cttgcaagct tgctgtagtt aagaagagca aggtaaagct cttcattgct acattctgca
    14281 atggttttat tgtaacgatt ttgtacaaat tcttgtagtg ataacatgtt tagttgtctc
    14341 ctgattattg tcttatttct ttaaatctac caaattttca ttaattcggc gataaattgt
    14401 tgtcaagtca agcaaacttt cctcgacagc tggtgtcaat tgatcttcag tcatacgcca
    14461 agaccagttt ccaccaaggg tagatgggaa gttcatacga gctgcctcat ccaattctag
    14521 taaatcttgc atagttgcaa ttgccataaa gctaactgat gaaaatactg tacgaagcat
    14581 agcgtgtacc actgtttcgt attctttacg gttcgtgtaa cgagccatgt actcacgagt
    14641 cgcatcatca atctcattac ggtaccaacc aagaaccgta ttgttatcgt gtgttcctgt
    14701 gtacataact gagttagcag gtgccaagtg tgggctatca atactttcgt cttctgggtt
    14761 gaaggcaaat tgaagaatct tcattcctgg gaagccagta cgttcacgca attcgatcac
    14821 ttcatctgtc atgaagccaa ggtcttctgc gatgatgttt agctcaccaa gttcttcctt
    14881 aacggctgca aaaagcttgt agcctggacc tttcacccac tcaccaggtg ctgctgtatc
    14941 ggaaccagca gggatttccc aataagattc gaagccacgg aagtggtcga tacgaacgat
    15001 atcgtagatt ttgaagcttt cacgcaagcg ttcaatccac catttgtagc cgtctttgtc
    15061 cattgcttcc cagtcataga ttgggttacc ccaaagctga ccagttacag aaaactcatc
    15121 tggtgggcat cctgcgatac aagtagcctt accattgaca tctgttttga agagatgtgg
    15181 atttgcccac atatcacttg aatcttccgc tacgtagatt ggcatgtccc caacgatttc
    15241 gatgtggttg tcgttagcgt aagctttcaa tttcaaccat tgttggaaga agaagtattg
    15301 agtcacacgg tggtaaacca acttgtctgc caattgctca cgatagcttt caagtgctga
    15361 agctttacga gcacgagcat ctgcatctgg ccattcagtc caagcaagat tgtcaaaatg
    15421 ctctttgata gccatatact cagcaaagag ctcaagccat gattggttgt cttgagcaaa
    15481 tttctcaaaa tctttaacat ctccgacttc aaagaaacgt ttcaccgctt tttctaaaag
    15541 aggacgacgt gcatagtaga ttttagcata gtcaacttca gacgcatcgc taccaaagtc
    15601 aactccttca aggtcacttg cttccaacaa accttgctcc accaagatat ctaaatcgat
    15661 aaaatgagtg tttcctgcga aggctgagaa agattggtaa ggagaatccc cgtaactagt
    15721 tgctcctaat ggaaggattt gccagtaacg ttgttttgta cggaccaaga aatcaacgaa
    15781 gtcgtaagca ctttgaccaa atgatccgat tccgtaagct cctggaagag aagagatgtg
    15841 catcaacaca ccactttgac gttttttcat aatagcacct cgtgtgttaa aataatggaa
    15901 cgttgcgtat tttttcagac gcaaacgttt gcgttactta taagtatact ccttttcaag
    15961 cataattgca agcgttttta aaaaactttt ttgattattt ttaaggagac taactcattt
    16021 tagcaaagat aacttaaaac tatctaatcc taaagtttga ctctcacgag acagtctaca
    16081 aagtacaaaa cctccttagg gagcttgaat ttatatgcaa caaagcacaa acgtctatag
    16141 aaaatagata tagattagaa acagggaggt agcccctcct gatttccctc tttagacaga
    16201 ttccatatat ttttattaaa aatactgtat tcttatctat ttcatacttt tcttctatta
    16261 aaatgcaaaa ttatgctatc aaatcaagct taaaaatttt ttaaaatttt tacaaaaaat
    16321 acttgcaacc gttttctatt tgtgctatac taagctcata aaggaaaacg tttgcgtttc
    16381 cttatcaata aaacttgcta ttctttagga ggaatacact atgtcatcta aatttatgaa
    16441 gagcgctgcg gtgcttggaa ctgctacact tgctagcttg ctcttggtag cttgcggcag
    16501 caaaactgct gataagcctg ctgattctgg ttcatctgaa gtcaaagaac tcactgtata
    16561 tgtagacgag ggatataaga gctatattga agaggttgct aaagcttatg aaaaagaagc
    16621 tggagtaaaa gtcactctta aaactggtga tgctctagga ggtcttgata aactttctct
    16681 tgacaaccaa tctggtaatg tccctgatgt tatgatggct ccatacgacc gtgtaggtag
    16741 ccttggttct gacggacaac tttcagaagt gaaattgagc gatggtgcta aaacagacga
    16801 cacaactaaa tctcttgtaa cagctgctaa tggtaaagtt tacggtgctc ctgccgttat
    16861 cgagtcactt gttatgtact acaacaaaga cttggtgaaa gatgctccaa aaacatttgc
    16921 tgacttggaa aaccttgcta aagatagcaa atacgcattc gctggtgaag atggtaaaac
    16981 tactgccttc ctagctgact ggacaaactt ctactataca tatggacttc ttgctggtaa
    17041 cggtgcttac gtctttggcc aaaacggtaa agacgctaaa gacatcggtc ttgcaaacga
    17101 cggttctatc gtaggtatca actacgctaa atcttggtac gaaaaatggc ctaaaggtat
    17161 gcaagataca gaaggtgctg gaaacttaat ccaaactcaa ttccaagaag gtaaaacagc
    17221 tgctatcatc gacggacctt ggaaagccca agcctttaaa gatgctaaag taaactacgg
    17281 agttgcaact atcccaactc ttccaaatgg aaaagaatat gctgcattcg gtggtggtaa
    17341 agcttgggtc attcctcaag ccgttaagaa ccttgaagct tctcaaaaat ttgtagactt
    17401 ccttgttgca actgaacaac aaaaagtatt atatgataag actaacgaaa tcccagctaa
    17461 tactgaggct cgttcatacg ctgaaggtaa aaacgatgag ttgacaacag ctgttatcaa
    17521 acagttcaag aacactcaac cactgccaaa catctctcaa atgtctgcag tttgggatcc
    17581 agcgaaaaat atgctctttg atgctgtaag tggtcaaaaa gatgctaaaa cagctgctaa
    17641 cgatgctgta acattgatca aagaaacaat caaacaaaaa tttggtgaat aaaaaatttg
    17701 ttcaaggggg gtggaaatca aatccccctt tgaatttatc aatagagaca caaataattt
    17761 agctttctta taaaaaagta gtatcctatg aaaggagtta atatggaaaa gcaacaacct
    17821 agtaaagcag ccctgctgtc tatcattcct gggttaggac agatttacaa taaacaaaaa
    17881 gccaaaggtt ttatcttcct tggtgtaacc atcgtatttg tcctttactt cctagcactt
    17941 gcaacccctg aattgagcaa cctcatcact cttggtgaca aaccaggtcg tgataattcc
    18001 ctctttatgc tgattcgtgg tgccttccat ctaatctttg taatcgttta tgtactcttt
    18061 tatttctcaa atatcaaaga tgcacatacg attgcaaaac gcattaacaa tggaattcca
    18121 gttccacgca cactcaaaga catgatcaaa gggatttatg aaaatggctt cccttacctc
    18181 ttgatcattc catcttatgt tgccatgacc ttcgcgatta tcttcccagt tatcgtaacc
    18241 ttgatgatcg cctttaccaa ctacgacttc caacacttgc caccaaacaa gttgttggac
    18301 tgggttggtt tgaccaactt tacaaacatt tggagcttga gtaccttccg ttctgccttt
    18361 ggttctgttc tttcttggac tatcatttgg gctttggcag cttctacttt acaaatcgta
    18421 attggtatct tcacagctat cattgccaac caaccattta tcaaaggaaa acgtatcttt
    18481 ggtgttattt tccttcttcc ttgggctgtc ccagccttca tcactatctt gacattctca
    18541 aacatgttta acgatagtgt cggtgctatc aacactcaag tattgccaat cttggctaaa
    18601 ttccttcctt tccttgatgg agctcttatt ccttggaaaa cagacccaac ttggactaag
    18661 attgccttga ttatgatgca aggttggctc ggattcccat acatctacgt tttgactttg
    18721 ggtatcttgc aatctattcc taacgacctt tacgaagcag cttatattga cggtgccaac
    18781 gcttggcaaa aattccgcaa catcactttc ccaatgattt tggctgttgc ggcacctact
    18841 ttgattagcc aatacacctt caactttaac aacttctcta tcatgtacct cttcaatggt
    18901 ggaggacctg gtagtgtcgg aggtggagct ggttcaaccg atatcttgat ctcatggatc
    18961 taccgtttga caacaggtac atctcctcaa tactcaatgg cggcagctgt taccttgatt
    19021 atctctatca ttgtcatctc aatctctatg atcgcattca agaaactaca cgcatttgat
    19081 atggaggacg tctaagatga ataactcaat taaactcaaa cgtagactga ctcaaagcct
    19141 tacttacctt tacctgattg gtctatcaat tgtaattatc tatccactgt tgattaccat
    19201 tatgtcagcc tttaaagcag gtaacgtctc agcctttaaa ctagatacta atatcgacct
    19261 caattttgat aactttaaag gcctcttcac tgaaaccttg tacggtactt ggtacctcaa
    19321 cactttgatt atcgccttaa ttaccatggc tgttcaaaca agtatcatcg tacttgccgg
    19381 ttatgcttac agccgttaca acttcttggc tcgtaaacaa agtttggtct tcttcttgat
    19441 catccaaatg gtgccaacta tggccgcttt gacagccttc ttcgttatgg cgcttatgtt
    19501 gaacgccctt aaccacaact ggttcctcat cttcctctac gttggtggtg gtatcccgat
    19561 gaatgcttgg ctcatgaaag gctacttcga tacagtgcca atgtctttag acgaatctgc
    19621 aaaactagac ggtgcaggac acttccgccg cttctggcaa attgttctac cacttgttcg
    19681 cccaatggtt gccgtacaag ctctctgggc cttcatggga cctttcgggg actacatcct
    19741 ctctagtttc ttgcttcgtg agaaagaata ctttactgtt gccgtaggtc tccaaacctt
    19801 cgttaacaat gcgaaaaact tgaagattgc ctacttctca gcaggtgcta tcctcatcgc
    19861 ccttccaatc tgtattctct tcttcttcct acaaaagaac tttgtttcag gacttacaag
    19921 tggtggcgac aagggataat ttatccccgc cacccttttt cattttatac tcttcgaaaa
    19981 tctcttcaaa ccacgtcagc tttatctcca acctcaaagt tgtgctttga gcaacctgtg
    20041 gctagtttgc actttgattt tcattgatta ttagcaattg tcactgtaaa taatatcctt
    20101 gtagcaagca atttttctcc tagacttgaa ataaagcgca tttcactata taataatact
    20161 catatagaaa acacctttta gaaagatacc tatgcttcca tatccatttt cctatttttc
    20221 aagtatttgg gggtttcgta agcccctgtc caaacgtttc gagctcaact ggtttcaact
    20281 tctctttacc agtatcttcc ttatcagctt gtctatggta cccattgcta tccaaaacag
    20341 ctcccaggag acctatccgc tagaaacttt tatcgataat gtctatgaac ctctgacaaa
    20401 tgaggttgtc caggatctct ctgaacatgc tacaattgtc gatggcacat taacttatac
    20461 tggaacagct agtcaaaccc cttctgttgt gattggtcca agtcaaatca aggaattacc
    20521 taaggacttg caactgcatt tcgatacaaa tgagctagtc atcagcaagg aaagcaagga
    20581 gctgacccgc atctcttacc gagccattca gactgagagt ttcaaaagca aagacagctt
    20641 gacccaagca atttctaaag actggtacca acaaaatcgt gtctatatca gcctcttcct
    20701 agttctcggt gcgagcttcc tctttggttt gaatttcttt atcgtctctc taggagctag
    20761 tcttctcctt tatatcacta agaaatcacg cctcttttca tttagaacct ttaaagagtg
    20821 ctatcacttt atcttgaact gtttaggatt gccgactctg attacactta ttttgggatt
    20881 atttggccaa aatatgacaa ccctgattac tgtacaaaat attctttttg ttctgtatct
    20941 ggtcactatc ttttataaaa cacatttccg tgatccaaat taccataaat aggagatttt
    21001 tatgcccgtt acgattaaag acgtggccaa ggctgctggt gtttcgcctt caaccgtaac
    21061 ccgtgttatt caaaataaat caaccattag cgacgaaaca aaaaaacgcg ttcgcaaagc
    21121 tatgaaggaa ctcaactacc acccaaacct caacgctcgt agcttggtaa gcagctatac
    21181 tcaggttatc ggattagttc ttcctgatga ctcagacgcc ttctaccaga atcctttctt
    21241 tccatcggtt ctacgtggca tctctcaagt cgcatctgaa aaccactatg ccattcagat
    21301 agcaacaggg aaagatgaga aggagcgtct caacgctatt tcacaaatgg tctacggcaa
    21361 gcgtgtagat gggctaatct ttctctatgc ccaagaagaa gaccctctcg taaaactcgt
    21421 cgcagaagaa cagttcccct tccttatcct aggtaaatct ctatctcctt tcatcccact
    21481 tgtcgacaac gacaatgttc aagctggttt tgatgcgact gaatatttca tcaaaaaagg
    21541 ctgcaaacgc attgccttta tcggaggaag taaaaagctc ttcgtgacca aagaccgttt
    21601 aacaggctat gaacaggcgc ttaaacatta caaacttacc actgacaaca atcgcatcta
    21661 ctttgccgac gagtttctgg aagaaaaggg ctataaattt agcaagcgat tattcaagca
    21721 cgatccacaa attgatgcta tcatcacaac cgatagcctc ctagctgaag gtgtttgtaa
    21781 ctatattgcc aaacaccagc tggatgtccc tgttctcagc tttgactcgg ttaatcccaa
    21841 gctcaacttg gcagcctatg tcgatatcaa tagtttagag cttggtcgtg tttcccttga
    21901 aactattctc cagattatta atgataataa aaacaataaa caaatttgtt accgtcaatt
    21961 gatcgcccac aaaattatcg aaaaataaga gactgggcaa agagtcgtta aaagcaaaaa
    22021 cgcatactat caggtattga aaaaacttga tactatgcgt tttattgtgg gaagatttac
    22081 ttccttttct actgaaattg agtcttttcc caagatcttt ttatactcaa tgaaaatcaa
    22141 agtgcaaact aggaagctag ccgcaggttg ctcaaaacac tgttttgagg ttgtggatag
    22201 aactgacgaa gtcagctcaa aacatggttt tgaggttgta gatgaaactg acg
//
LOCUS       DAVLVZ010000027        22196 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000027 DAVLVZ010000000
VERSION     DAVLVZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22196
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.027"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            143..1246
                     /gene="glf"
                     /locus_tag="VYN77_001087"
     CDS             143..1246
                     /gene="glf"
                     /locus_tag="VYN77_001087"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEV1558559.1"
                     /translation="MYDYLVVGAGLFGAVFAHEAALKGKKVKVIEKRNHIAGNIYTRE
                     EEGIQVHQYGAHIFHTSDKEIWDYVNQFAEFNRYTNSPVANYKGEIYNLPFNMNTFNK
                     LWGVVTPAEAQAKIEEQRAILNGKTPENLEEQAISLVGTDIYEKLIKDYTEKQWGKPT
                     TELPSFIIRRLPVRLTYDNNYFNDTYQGIPIGGYTQIVEKMLDHENIDVETNVDFFVN
                     KEQYLKDFPKIVFTGMIDEFFDYKLGELEYRSLRFENETLDMENYQGNAVVNYTDAET
                     PYTRIIEHKHFEFGSQAKTIITKEHSKTWEKGDEPYYPVNNDRNNHLYKSYKKLADEQ
                     GNVIFGGRLGHYRYYDMHQVIGVALQCVRNELN"
     gene            1567..3549
                     /locus_tag="VYN77_001088"
     CDS             1567..3549
                     /locus_tag="VYN77_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1558560.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQIGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            3850..9153
                     /locus_tag="VYN77_001089"
     CDS             3850..9153
                     /locus_tag="VYN77_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1558561.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKTAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTTLVAED
                     FASLDAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TNNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(9320..11479)
                     /locus_tag="VYN77_001090"
     CDS             complement(9320..11479)
                     /locus_tag="VYN77_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1558562.1"
                     /translation="MNKQTILRIAKYVSICFLTLFITAVMLGGGIFLYYVSKAPALSE
                     SKLVATTSSKIFDSKNELIADLGSERRVNAQANEIPTDLVKAIVSIEDHRFFDHRGVD
                     TIRIIGAFLRNLQNNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAVQLEQK
                     TTKQEILTYYVNKVYMSNGNYGMQTAAQNYYGKDLRELSLPQLALLAGMPQAPNQYDP
                     YSHPEAALDRRNLVLSEMKGQGYISAEQYEKAINTPITDGLQSLKSVNSYPVYMDNYL
                     KEVIDQVEQETGYNLLTTGMDVYTNVDQEAQKRLWDIYNSDQYVSYPDDDLQVASTVV
                     DVSNGKVIAQLGARHQASNVSFGTNQAVETNRDWGSSMKPITDYAPALEYGVYDSTAT
                     MVNDIPYNYPGTSTPVYNWDRAYFGNISLQYALQQSRNVPAVETLNKVGLDRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESSKQYGASSEKMAAAYAAFANGGIYHKPMYINKVVFS
                     DGSEKEFSDAGTRAMKETTAYMMTEMMKTVLTYGTGRGAYLPWLPQAGKTGTSNYTDD
                     EIEKYIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIIGDGFLVAAKVYRSMISY
                     LSEDDHPGDWTMPEGVYRSGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     LTTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(11476..12072)
                     /gene="recU"
                     /locus_tag="VYN77_001091"
     CDS             complement(11476..12072)
                     /gene="recU"
                     /locus_tag="VYN77_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1558563.1"
                     /translation="MVNYPHKLSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVRVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEAKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLGYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            12139..12666
                     /locus_tag="VYN77_001092"
     CDS             12139..12666
                     /locus_tag="VYN77_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1558564.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGTLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            12736..13077
                     /gene="gpsB"
                     /locus_tag="VYN77_001093"
     CDS             12736..13077
                     /gene="gpsB"
                     /locus_tag="VYN77_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1558565.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYSKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELARKPQVSSAPSPSHPDPIDVAASSSMTNFDILKRLNRLEKEV
                     FGKQILDNPDL"
     gene            13098..13478
                     /gene="rnpB"
                     /locus_tag="VYN77_001094"
     ncRNA           13098..13478
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYN77_001094"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13563..14720
                     /locus_tag="VYN77_001095"
     CDS             13563..14720
                     /locus_tag="VYN77_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1558566.1"
                     /translation="MKKEFNLIATAAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     TIIETNLWLRAADRIKIIVGTFQAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            14733..16127
                     /gene="mapZ"
                     /locus_tag="VYN77_001096"
     CDS             14733..16127
                     /gene="mapZ"
                     /locus_tag="VYN77_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1558567.1"
                     /translation="MSKKRRNRHKKEAQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSHLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSLSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            16203..17627
                     /gene="gndA"
                     /locus_tag="VYN77_001097"
     CDS             16203..17627
                     /gene="gndA"
                     /locus_tag="VYN77_001097"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1558568.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            17639..18328
                     /locus_tag="VYN77_001098"
     CDS             17639..18328
                     /locus_tag="VYN77_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1558569.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            18427..19449
                     /gene="cbpC"
                     /locus_tag="VYN77_001099"
     CDS             18427..19449
                     /gene="cbpC"
                     /locus_tag="VYN77_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1558570.1"
                     /translation="MKLLKKMMQVALAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDAENGDMKIGWTKVNGKWYYLNSSGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            19468..20466
                     /gene="cbpJ"
                     /locus_tag="VYN77_001100"
     CDS             19468..20466
                     /gene="cbpJ"
                     /locus_tag="VYN77_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEV1558571.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLKGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKYSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGLMATGWYQEGTTWYYLDQSNGDMKTGWQYLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(20584..21816)
                     /locus_tag="VYN77_001101"
     CDS             complement(20584..21816)
                     /locus_tag="VYN77_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1558572.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(21859..>22196)
                     /locus_tag="VYN77_001102"
     CDS             complement(21859..>22196)
                     /locus_tag="VYN77_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEV1558573.1"
                     /translation="NLQLSWCKGISGIILYLCMYDCDGNKDIISKYQEFVFNHHLKMM
                     TGYCHGITSLLQTTVYNQNKLLMKKIQQVILACSERDDHGLLMFQGDSGKADLFDFGI
                     GSMGYIGVY"
BASE COUNT         6992 a         3957 c         4983 g         6264 t
ORIGIN      
        1 ggaaaaacta tttggtttta attttatagg ttcatattta ctttttgcta tatttacttt
       61 aagtgttagt tttatcattg ttggaatgtt aatgaaaatt ccgtatttta atcgaatcgt
      121 caaattataa aaaggagaaa aaatgtacga ttatcttgtt gttggtgctg gtctctttgg
      181 tgcagtcttt gcccatgaag cagccttaaa aggaaaaaaa gtaaaagtta ttgaaaaacg
      241 aaatcatatc gcgggtaata tctatactcg tgaagaggaa ggaattcaag ttcatcaata
      301 tggtgctcat atcttccata cttctgataa ggagatctgg gattatgtaa atcagtttgc
      361 agagtttaac cgttatacca attctcctgt tgcaaactat aagggtgaga tttataacct
      421 tccttttaat atgaatactt tcaataaact ttggggagtt gtaacgccag cagaagcaca
      481 agctaagatt gaggaacaac gtgctatttt aaatggtaaa actcctgaaa atttggaaga
      541 acaggcgatt tctcttgtag gtacagacat ctacgaaaaa ttaatcaaag actatacaga
      601 gaaacagtgg ggcaaaccaa ctactgaact tccatccttt attattcgcc gtttgccagt
      661 acgcctgacc tatgataaca actattttaa cgatacctat caagggattc caattggtgg
      721 atacactcaa atagttgaaa aaatgttgga tcatgaaaat attgatgtag aaacaaatgt
      781 tgatttcttt gtgaataaag agcaatatct gaaagatttt cctaagattg tctttactgg
      841 tatgattgat gaattctttg actataagtt gggcgaacta gagtaccgta gtcttcgttt
      901 tgaaaatgag accttggata tggaaaatta ccaaggaaat gcagttgtga actatacgga
      961 tgcagaaact ccatatactc gcattattga acacaaacat tttgagtttg ggagtcaagc
     1021 aaagactatc attactaaag aacattctaa aacatgggaa aaaggtgatg agccttatta
     1081 tccagttaat aatgatcgta ataatcattt gtataaatca tacaaaaaac ttgctgatga
     1141 gcaagggaat gttatctttg gtggccgctt aggacactat cgttattacg atatgcacca
     1201 agtaattgga gtagctttgc agtgcgtgag aaatgagtta aattaatact caatgaaaat
     1261 taaagagcaa actaggaagc tagccacagg ttgctcaaaa cactgttttg aggttgcaga
     1321 tggacgctga cgcggtttga agagattttc gaagagtata aacaagtaaa actgactacc
     1381 agttattatt tagaaatagt attaaaaatt ccttgactat gtgatatagt tgagggattt
     1441 ttaaatgata ttcatatttt ttgcaaagat gttgtttgaa aaataatttt caaaaattct
     1501 gaaaattctg ttgacaactt tctgaaaaga gtctataatg gagagaaagt tttaaaggag
     1561 aaaatgatga aaagttcaaa actatttgcc cttgcgggcg tgacattatt ggcggcgact
     1621 actttagctg catgctctgg atcaggttca agcactaaag gtgagaaaac attctcatac
     1681 atttatgaga cagaccctga taacctcaac tatttgacaa ctgctaaggc tgcgacagca
     1741 aatattacca gtaacgtggt tgatggtttg ctagaaaatg atcgctacgg gaactttgtg
     1801 ccgtctatgg ctgaggattg gtctgtatcc aaggatggat tgacttacac ttatactatc
     1861 cgtaaggatg caaaatggta tacttctgaa ggtgaagaat acgcggcagt caaagctcaa
     1921 gactttgtaa caggactaaa atatgctgct gataaaaaat cagatgctct ttaccttgtt
     1981 caagaatcaa tcaaagggtt ggatgcctat gtaaaagggg aaatcaaaga tttctcacaa
     2041 gtaggaatta aggctctgga tgatcagaca gttcagtaca ctttgaacaa accagaaagt
     2101 ttttggaact caaaaacaac catgggtgtg cttgcgccag ttaatgaaga gtttttgaac
     2161 tcaaaagggg atgattttgc caaagcgacg gacccaagta gtctcttgta taatggacct
     2221 tatttgttga aatccattgt gacaaaatcc tctgttgaat ttgcgaaaaa tccgaactac
     2281 tgggataagg acaatgtgca tgttgacaaa gttaaattgt cattctggga tggtcaagat
     2341 accagcaaac ctgcagaaaa ctttaaagat ggtagcctta cagcagctcg tctctatcca
     2401 acaagtgcaa gtttcgcaga gcttgagaag agtatgaagg acaatattgt ctatactcaa
     2461 caagactcta ttacgtatct agttggtaca aatattgacc gtcagtccta taaatacaca
     2521 tctaagacca gcgacgaaca aaaggcatcg actaaaaagg ctctcttaaa caaggatttc
     2581 cgtcaggcta ttgcctttgg ttttgatcgt acagcctatg cctctcagtt gaatggacaa
     2641 attggagcaa gtaaaatctt gcgtaatctc tttgtgccac caacatttgt tcaagcagat
     2701 ggtaaaaact ttggcgatat ggtcaaagag aaattggtca cttatgggga tgaatggaag
     2761 gatgttaatc ttgcagattc tcaggatggt ctttacaatc cagaaaaagc caaggctgaa
     2821 tttgctaaag ctaaatcagc cttacaagca gaaggtgtga ctttcccaat tcatttggat
     2881 atgccagttg accagacagc aactacaaaa gttcagcgcg tccaatctat gaaacaatcc
     2941 ttggaagcaa ctttaggagc tgataatgtc attattgata tccaacaact acaaaaagac
     3001 gaagtaaaca atattacata ttttgctgaa aatgctgctg gcgaagactg ggatttatca
     3061 gataatgtcg gttggggtcc tgattttgcc gatccatcaa cttaccttga tatcatcaaa
     3121 ccatctgtag gagaaagtac taaaacatat ttagggtttg actcagggga agataacgta
     3181 gctgctaaaa aagtaggtct atatgactat gaaaaattgg ttactgaggc tggtgatgag
     3241 gctacagatg ttgctaaacg ctatgataaa tacgctgcag cccaagcttg gttgacagat
     3301 agtgctttga ttattccaac tacatctcgt acagggcgtc caatcttgtc taagatggta
     3361 ccatttacaa taccatttgc attgtcagga aataaaggta caagtgaacc agtcttgtat
     3421 aaatacttgg aacttcaaga caaggcagtc actgtagatg aataccaaaa agctcaggaa
     3481 aaatggatga aagaaaaaga agagtctaat aaaaaggctc aagaagatct cgcaaaacat
     3541 gtgaaataac tgttgcaaaa tataagaaag gatttagtat ttctcttgaa tgctgaatcc
     3601 ttttttacat ttgtaaagaa agattctaaa tgtactgacc cccaaaagtt ggagcctctt
     3661 tttgtcagaa tagagaaaat ttttgttaat tttacttgtt tcctattgct ttctcagcta
     3721 ttgtttgtta tattaaaagc ataattattt tttatttatc agggttaagc attgcacttt
     3781 cagaggaagg agtatttttt aaaaagaaaa tgtaaacgct tgctcaaaaa tgaaaggatt
     3841 tagaagttta tgaataaagg attatttgaa aaacgttgta aatatagtat tcggaaattt
     3901 tcattaggtg ttgcttctgt tatgattgga gctgcattct ttgggacaag tccggttctt
     3961 gcagatagcg tgcagtctgg ttccacggcg aacttaccag ctgatttagc tactgctctt
     4021 gcaacagcaa aagagaatga tggacgtgat tttgaagcgc ctaaggtggg agaagaccaa
     4081 ggttctccag aagttacaga tggacctaag acagaagaag aactattagc acttgaaaaa
     4141 gaaaaaacgg ctgaagaaaa accaaaagag gataaacctg cagctgctaa acctgaaacg
     4201 cctaagacgg taacccctga atggcaaacg gtagagaaaa aagaacaaca gggaacagtc
     4261 actatccgag aagaaaaagg tgtccgctac aaccaactat cctcaactgc tcaaaatgat
     4321 aacgcaggta aaccagccct gtttgaaaag aagggcttga ccgttgatgc caatggaaat
     4381 gcaactgttg atttaacctt caaagaagat tctgaaaagg gcaaatcacg ctttggtgtc
     4441 ttcttgaaat ttaaagatac caataataat gtttttgtcg gttatgacaa ggatggctgg
     4501 ttctgggagt ataaatctcc aacaactagc acttggtata gaggtagtcg tgttgctgct
     4561 cctgaaacag gatcaacaaa ccgtctctct atcactctca agtcagacgg tcagctaaat
     4621 gccagcaata acgatgtcaa tctctttgac acagtgactc taccagctgc ggtcaatgac
     4681 catcttaaaa atgagaagaa gattcttctc aaggcgggct cttatgacga tgagcgaaca
     4741 gttgttagcg ttaaaacgga taaccaagag ggggtaaaaa cagaggatac ccctgctgaa
     4801 aaagaaacag gtcctgaagt tgatgatagc aaggtgactt atgacacgat tcagtctaag
     4861 gtcctcaaag cagtgattga ccaagccttc cctcgtgtca aggaatacag cttgaacggg
     4921 catactttgc caggacaggt gcaacagttc aaccaagtct ttatcaataa ccaccgaatc
     4981 acccctgaag tcacttataa gaaaatcaat gagacaacag cagagtactt gatgaagctt
     5041 cgcgatgatg ctcacttaat caatgcggaa atgacagtac gcttgcaagt tgtggacaat
     5101 caattgcact ttgatgtgac caagattgtc aaccacaatc aagtcactcc aggtcaaaag
     5161 attgatgacg aaagaaaact actttcttct attagtttcc tcggcaatgc tttagtctct
     5221 gtttctagtg atcaaactgg tgctaagttt gatggggcaa ctatgtcaaa caatacgcat
     5281 gtcagcggag atgatcatat cgatgtaacc aatccaatga aggatttggc taagggttac
     5341 atgtatggat ttgtttctac agataagctt gctgctggtg tttggagtaa ctctcaaaac
     5401 agctatggtg gtggttcgaa tgactggact cgtttgacag cttataaaga aacagtcgga
     5461 aatgccaact atgtaggaat ccacagctct gaatggcaat gggaaaaagc ttataagggc
     5521 attgttttcc cagaatacac gaaggaactt ccgagtgcta aggttgttat cactgaagat
     5581 gccaatgcag acaagaaagt tgattggcaa gatggtgcca ttgcttatcg tagcattatg
     5641 aacaaccctc aaggttggga aaaagttaag gatatcacag cttaccgtat cgcgatgaac
     5701 tttggttctc aagcacaaaa cccattcctt atgaccttgg atggtatcaa gaaaatcaat
     5761 ctccacacag atggtcttgg gcaaggtgtt ctccttaaag gatatggtag cgaaggccat
     5821 gactctggtc acttgaacta tgctgatatt ggtaagcgta tcggtggtgt cgaagacttc
     5881 aagaccctaa ttgagaaggc taagaaatat ggagctcatc taggtatcca cgttaacgct
     5941 tcagaaactt atcctgagtc taaatacttc aatgaaaaaa ttctccgtaa gaatccagat
     6001 ggaagctata gctatggttg gaactggcta gatcaaggta tcaacattga tgctgcctat
     6061 gacctagctc atggtcgttt ggcacgttgg gaagatttga agaaaaaact tggtgacggt
     6121 ctcgacttta tctatgtgga cgtttggggt aatggtcaat caggtgataa cggtgcctgg
     6181 gctacccacg ttcttgctaa agaaattaac aaacaaggct ggcgctttgc gatcgagtgg
     6241 ggccatggtg gtgagtacga ctctaccttc catcactggg cagctgactt gacctacggt
     6301 ggctacacca ataaaggtat caacagtgcc atcacccgct ttatccgtaa ccaccaaaaa
     6361 gatgcttggg ttggggacta cagaagttat ggtggtgcag ccaactatcc actgctaggt
     6421 ggctacagca tgaaagactt tgaaggctgg caaggaagaa gtgactacaa tggctatgta
     6481 accaacttat ttgcccatga cgtcatgacc aagtatttcc aacacttcac tgtaagtaaa
     6541 tgggaaaatg gtacaccggt gactatgacc gataacggta gcacctataa atggactcca
     6601 gaaatgcgag tggaattggt agatgctgac aataataaag tagttgtaac tcgtaagtca
     6661 aatgatgtca atagtccaca atatcgcgaa cgtacagtaa ctctcaacgg acgtgtcatc
     6721 caagatggtt cagcttactt gactccttgg aactgggatg caaatggtaa gaaactttct
     6781 actgagaagg aaaagatgta ctacttcaat acgcaggccg gtgcaacaac ttggaccctt
     6841 ccaagcgatt gggcaaagag caaggtttac ctttacaagc taactgacca aggtaagaca
     6901 gaagagcaag aactaactgt aaaagatggt aaaattaccc tagatcttct agcaaatcaa
     6961 ccatacgttc tctatcgttc gaaacaaacc aatcctgaaa tgtcatggag cgaaggcatg
     7021 cacatctatg accaaggatt taatagtgga accttgaaac actggaccat ttctggtgat
     7081 gcttctaagg cagaaattgt caagtctcaa ggggcaaacg atatgcttcg tattcaagga
     7141 aacaaagaaa aagttagtct cactcagaaa ttaactggct tgaaaccaaa tactaagtat
     7201 gccgtttatg tcggtgtcga taaccgtagt aatgccaagg caagtatcac tgtgaatact
     7261 ggtgaaaaag aagtgactac ttataccaat aagtctctcg ccctcaacta tgttaaggct
     7321 tacgcccaca atacacgtcg tgacaatgct acagttgacg atacaagtta cttccaaaac
     7381 atgtacgcct tctttacaac tggatcggac gtatcaaatg ttactctgac attgagtcgt
     7441 gaagctggtg atcaagcaac ttactttgat gaaattcgta cctttgaaaa caattcaagc
     7501 atgtacggag acaagcatga tacaggtaaa ggcaccttca agcaagactt tgaaaatgtt
     7561 gctcagggta tcttcccatt tgtagtgggt ggtgtcgaag gtgtcgaaga caaccgcact
     7621 cacttgtctg aaaaacacga tccatataca caacgtggtt ggaatggtaa gaaagtcgat
     7681 gatgttatcg aaggaaattg gtcactcaag acaaatggac tagtgagccg tcgtaacttg
     7741 gtttaccaaa ccatcccaca aaacttccgt tttgaagcag gtaagaccta ccgtgtaacc
     7801 tttgaatacg aagcaggttc agacaatacc tatgcttttg tagtcggtaa gggagaattc
     7861 cagtcaggtc gtcgtggtac tcaagcaagc aacttggaaa tgcatgaatt gccaaatact
     7921 tggacagatt ctaagaaagc caagaaggca accttccttg tgacaggtgc agaaacaggg
     7981 gatacttggg taggtatcta ctcaactggc aatgcaagta atactcgtgg tgattctggt
     8041 ggaaatgcca acttccgtgg ttataacgac ttcatgatgg ataatcttca aatcgaagaa
     8101 attaccctaa caggtaagat gttgacagaa aatgctctga agaactactt gccaacggtt
     8161 gccatgacta actacaccaa agagtctatg gatgctttga aagaggcggt ctttaacctc
     8221 agtcaggccg atgatgatat cagtgtggaa gaagcgcgtg cagagattgc caagattgaa
     8281 gccttgaaga atgctttggt tcagaagaaa acgactttgg tagcagaaga ctttgcaagt
     8341 ttggatgcgc cagctcaggc tcaagaaggt cttgcaaatg cctttgatgg aaacttatct
     8401 agtttatggc atacatcatg gggcggagga gatgtaggca agcctgcaac catggtcttg
     8461 aaagaagcaa ctgaaatcac tggacttcgt tatgttccac gtggatcagg ttcaaatggt
     8521 aacttgcgtg atgtgaaact tgttgtgaca gatgagtctg gcaaggagca tacctttact
     8581 gcaactgatt ggccagataa caataagcca aaagacattg attttggtaa gacaattaag
     8641 gctaagaaaa ttgtccttac aggtactaag acttacggag atggtggcga taagtaccaa
     8701 tctgcagcgg aactcatctt tactcgtcca caggtagcag aaacacctct tgacttgtca
     8761 ggctatgaag cagctttggc taaggctcag aaattaacag acaaagacaa tcaagaggaa
     8821 gtagctagcg ttcaggcaag catgaaatat gcgacgaata accatctctt gacggaaaga
     8881 atggtggaat actttgcaga ttatctcaac caattaaaag attctgctac gaaaccagat
     8941 gctccaactg tagagaaacc tgagtttaaa cttagctctt tagcttccga tcaaggtaaa
     9001 acgccagatt ataagcaaga aatagctaga ccagaaacac ctgaacaaat cttgccagca
     9061 acaggtgaga gtcaatctga cacagccctc ttcctagcag gtgttagcct agccctatct
     9121 gctctctttg tagtaaaaac gaagaaagac tagtatttag taaaacctct taacaagatt
     9181 acggaagcag tctctatctt ttccaatgag gtttatagta cagaaaaagc ctgagaagat
     9241 gtcttctcag gcttttgtta agcacataaa tacaatagtg ctatgacaaa atcacccaga
     9301 aaaatctggg tgataaatgt tatggttgtg ctggttgagg attctgattt tgttgatcag
     9361 gggttgtatt tgattgttgc gtattgttgt taggattggt agtcgtacta ttatttgtgc
     9421 ttggagtggt taagctagac tgtgaagttg aactatctga tgatgagctt gaactttcag
     9481 ttgatggggg ttgttgtgga gcaggggagc tccacgtaga acgagcacca tttttaaata
     9541 cgaattctcc acttctgtat actccctcag gcattgtcca atctccagga tggtcatctt
     9601 ctgatagata cgatatcatt gagcgataaa ctttggcagc aactaggaaa ccatctccaa
     9661 tgattggagt taaacgattt gagtatccag tccatacagc catagaatac ttacgagtat
     9721 aacccacaaa catttcatct ggagctacgt agccagtgtt cttgatatac ttttcaattt
     9781 cgtcgtcagt atagttagaa gtacctgtct tacctgcttg tggaagccat ggtaggtagg
     9841 ctccacgtcc tgttccgtaa gttaaaacag ttttcatcat ttcagtcatc atataggcag
     9901 tagtctcttt catagctcgt gtaccagcat cagaaaattc tttttcgcta ccatcactaa
     9961 agacaacttt attgatgtac attggtttgt ggtaaatacc gccattagcg aatgcagcgt
    10021 aagccgcagc cattttctca ctgcttgccc cgtactgttt actagattca gttgtattac
    10081 ttgaaatagc atttgagtag tgaatacttg gatagtcaat tcccaaacca tttaggaaag
    10141 ttttggctct atctaaacca accttgttta gtgtttcaac ggcaggcacg ttacgagatt
    10201 gttgaagggc atattgcagg ctaatattac caaaatatgc tcgatcccag ttgtagacag
    10261 gtgtgcttgt gcccggatag ttataaggaa tatcattaac catagttgca gtggaatcat
    10321 atacaccata ttctaaggct ggggcataat cagtgattgg tttcatgctt gatccccagt
    10381 cacggttggt ttccacagct tggttggtac caaatgaaac gttacttgct tggtgacgag
    10441 ctccaagttg ggcgatgact ttaccatttg aaacatctac gaccgtagat gcgacttgca
    10501 aatcatcgtc agggtaagag acgtattgat cggagttgta gatatcccac agacgttttt
    10561 gagcctcttg gtctacattt gtgtagacat ccatcccagt agttagaagg ttatagccag
    10621 tttcttgttc tacttggtcg ataacctctt tgagataatt gtccatatat actgggtagc
    10681 tattgactga tttcaaactt tggagtccat cagtaatagg agtattgata gccttctcat
    10741 actgttcggc agaaatgtag ccttgacctt tcatctctga aagtaccaag ttacgacggt
    10801 caagggcagc ttctggatga gaatagggat cgtattgatt cggagcctgt ggcattcctg
    10861 ctagtagggc aagttgaggt agtgatagtt ctctcaaatc tttgccgtaa tagttttggg
    10921 cagccgtctg cattccatag ttgccgttag acatgtaaac tttattcacg tagtaggtca
    10981 gaatttcttg ttttgtggtt ttttgttcta attgaacagc caaccaagct tcctgggctt
    11041 tacgtgaaat ggtctgatca gaagtggaag ttgagaaata ggtcaactta attaattgtt
    11101 gggtaagggt tgatcctccc tggagagagt tgttttgtaa gttacgtaaa aaagctccta
    11161 taattcggat ggtatcgaca cctcgatgat cgaaaaaacg atggtcttcg attgatacga
    11221 tagccttgac caaatcagta ggaatttcgt tggcctgagc gtttactcgg cgttcagagc
    11281 ccagatcggc aatcagttcg tttttactat cgaaaatctt actagacgtg gttgcgacta
    11341 atttgctctc agatagggct ggggctttac tgacgtaata gagaaatatg cctcctccca
    11401 gcataactgc tgtgataaat aaagttaaga agcagatact cacatactta gctattcgca
    11461 ggatagtttg tttgttcatc ttgttttacc acctaataaa tgttctttga taacattgag
    11521 atagggaatt tgagggaagg caccagcctt gatttcatat ccatattctc gaatatatcc
    11581 aagtggcatt gatttttgtc ccttatcttg atggtagaag cgaatcaaat cgaatgccgg
    11641 caataagtag gtttcttgct gagaagaaaa gtgaagaagg acaaagcaga ttccttgttg
    11701 ggcaaggact tgttccatat gctgaatttg atgtggatga aaatttttca tcggaatcgc
    11761 acgtttttgt tttgtttcct tggcttcaaa gtcgatgtaa tatccattat aaacgccaga
    11821 atagtccgtc gttgaagctt gtcgaaaata ggcctcaaca atcttggcac gacttcgctg
    11881 tggatagtcc actcgtacaa tttgaatagg ggttggtttc ttgtgtataa cagccaagcc
    11941 ctgagacaaa tagtagtcgt tggtagcatt gatcatcttt tcaaaagaca ttcctcgatt
    12001 tgcgaaattt ttgggttgag aaagagatgt ttgtcttttt tgtgatgaaa gtttatgtgg
    12061 atagttgacc ataattctcc ttattggtac aataacatca ctctattata ccataaattt
    12121 gcacagaaag ggttaaaaat ggctacagct ttggttttag gctattctgc ttttgatttg
    12181 ggtttatttt ctgataagga tcctcgtctt aaattgatta aaaaagcgat acgtaaagat
    12241 ttagaggcta tggcagcaga tggggtgtct tggcttgtat ttacagggac tttgggcttt
    12301 gaatattggg ttttagaggt tgctcaggag atgaaaaccg agtacggttt ccagttggcc
    12361 accatttttg cttttgaaac ccatggggaa aattggaatg aaggcaatca gatgaaactg
    12421 agtcgtttta agcaggtaga ctttgtcaaa tatgcttatc ctcgctatga gcacaagggg
    12481 cagttaagag attaccagca gtttttgctg gaaaacacga ctagttccta tcttttttat
    12541 gatgaagaaa atgaaacgaa actagcttat ttttaccaaa agatgaaaaa tcaagaggac
    12601 tattttataa agagattaac atttgatcaa ttaaatgaac ttgctgaaaa tttttccgaa
    12661 aattgaagct ttgaccttga tttttgtttg ccttttttta tataataata ctagcaagcg
    12721 agaatggaga gagacatggc aagtattatt ttttcagcga aagatatttt tgaacaagag
    12781 tttggacgtg aagtccgagg gtatagtaag gtagaagttg acgagttttt agacgatgtc
    12841 atcaaggact atgaaaccta tgctgccttg gtcaagtcac ttcgtcagga aattgcagat
    12901 ttgaaggaag aattagcccg taaaccgcaa gtgagttcag ccccaagtcc tagtcaccca
    12961 gatccaattg atgtggcagc ttcatcttct atgacgaatt ttgatatttt gaaacgcttg
    13021 aatcgtcttg aaaaagaagt attcggcaaa caaattttag acaatcctga tttgtaattc
    13081 aggtcttgtc acatgcaatt tttggataat cgcgtgagga gagttgcttc tcatgaggaa
    13141 agtccatgct agcacaggct gtgatgcctg tagtgtttgt gctaggcgaa accataagcc
    13201 tagggacgag taatcgttac ggcagttgaa atggctaagt cctcggatag gtcagagtag
    13261 gcttgaaagt gccacagtga cggagtcttt ctggaaacgg agagagtgga acgcggtaaa
    13321 cccctcaagc tagcaaccca aattttggtc ggggcatgga gtacgcggaa acgaacgtag
    13381 tattctgact gctatcagct agagttgtta gtggtagaca gatgattatc gaaggaagtg
    13441 gtcctagtca cttctggaac aaaacatggc ttatagaaaa ttgcatatag gttggggctg
    13501 agaaaacttt ctcaacctca ttttttaaag tggacatata gaaaggtctt ataagactga
    13561 aaatgaaaaa agaatttaat ttaatcgcta ctgctgcagc gggtcttgag gctgtcgttg
    13621 gtcgtgaagt gcgagagttg ggctacgatt gtcaggttga aaatggacgt gttcgttttc
    13681 aaggagacgt gagaactatt atcgaaacca acctctggct tcgggcagca gatcgtatca
    13741 aaatcatcgt aggaaccttc caagctaaga cttttgaaga gctgtttcag ggagttttcg
    13801 ctctagattg ggaaaattat ttaccactcg gagctcggtt cccgatttca aaagctaaat
    13861 gtgttaagtc caaacttcac aatgagccca gtgttcaggc tatttctaag aaagctgttg
    13921 tcaagaaatt gcagaaacac tatgctcgcc cagaaggggt tcctctgatg gagaatggcc
    13981 cagagtttaa gattgaggtc tctattctca aagatgtggc aactgtcatg attgatacga
    14041 ccgggtctag cctctttaaa cgtggttatc gtaccgaaaa aggtggcgct cctatcaagg
    14101 aaaatatggc agcagccatt ttacaacttt ctaactggta tccagacaag cctttgattg
    14161 atccgacctg tggttcgggg actttctgta ttgaggcagt tatgattgct agaaagatgg
    14221 cgccaggtct tcgtcgctct tttgcatttg aggaatggaa ctggatcagc gatcgcttga
    14281 ttcaagaagt gcgcacagaa gcggctaaaa aagtagaccg tgagcttgag ctggatatca
    14341 tgggctgtga tattgatgct cgcatggtgg aaattgctaa ggccaatgct caggtagctg
    14401 gtgttgcagg agacattact tttaagcaga tgcgcgtgca ggatttacgt tccgataaaa
    14461 tcaatggagt aatcatttcc aatccgcctt atggtgaacg tttgtcagat gatgcagggg
    14521 tgaccaagct ctatgctgag atggggcaag tatttgcacc gctgaaaact tggagcaaat
    14581 ttatcctgac tagtgatgaa gcttttgaaa gcaagtatgg tagccaagca gataagaagc
    14641 gtaagttata caacggaacc ttgaaagtgg atctatatca atattttggt cagcgtgtca
    14701 aacggcaaga ggtaaaatag aaagggatac tcatgagtaa aaaaagacga aatcgtcata
    14761 aaaaagaagc tcaagaaccg caatttgatt ttgatgaagc aaaagagcta acagttggtc
    14821 aagctattcg taaaaatgaa gaagtggaag caggagtctt gcctgaggat tccattttgg
    14881 acaagtatgt taagcaacac agagatgaaa ttgaggcgga taagtttgcg actcgtcaat
    14941 acaaaaaaga ggagttcgtt gaaactcaga gtctggatga tttaattcaa gagatgcgtg
    15001 aggctgtaga gaagtcagaa gcttcttcgg aggaagttcc atcttctgaa gacatcttac
    15061 tacccttgcc tctggacgat gaggagcaag gcttggatcc tctattgcta gatgatgaaa
    15121 atccaacaga aatgactgaa gaagtggaag aggagcaaaa cctttctcat ctggatcaag
    15181 aggactcaga aaagaaaagt aaaaaaggct ttattttgac cgttttggcg cttgtatcag
    15241 taattatttg tgtcagtgct tattatgtct accgtcaagt ggctcgttcg accaaggaaa
    15301 ttgaaacttc tcaatcaact acagccaatc aatcggatgt ggatgatttt aatacacttt
    15361 atgacgcctt ttacacagat agcaataaaa cggctttgaa aaatagccag tttgataaac
    15421 tgagtcaact caaaaccttg cttgataaac tggaaggtag tcgtgaacat acgcttgcca
    15481 aatctaaata tgatagtcta gcaacgcaaa tcaaggctat tcaagatgtc aatgcacaat
    15541 ttgaaaaatc agctattgtg gatggtgtgt tggataccaa tgccaaagcc aaatcggatg
    15601 ctaaatttac agatattaaa actggaaata cggagcttga taaagtgcta gataaggcta
    15661 tcagtcttgg taagagccag caaacaagta cttctagctc aagtttaagt caaactagca
    15721 gctcaagttc aagtcaagca agttcaaata cgactagtga gccaaaacca agtagttcaa
    15781 atgagactag aagtagtcgc agtgaagtca atatgggtct ctcgagtgca ggggttgctg
    15841 ttcaaagaag tgccagtcgt gttgcctata atcagtctgc tattgatgat agtaataact
    15901 ctgcctggga ttttgcggat ggtgtcttgg aacaaattct agcgacttca cgttcacgtg
    15961 gctatatcac tggagaccaa tatatccttg aacgtgtcaa tatcgttaac ggcaatggtt
    16021 attataacct ctataagcca gatggaacct atctctttac ccttaactgt aagacaggct
    16081 actttgtcgg aaatggcgct ggtcatgcgg atgacttgga ctactaagca gtcgttacaa
    16141 aattctttct tttcaaaagt aaaaatgata aaataaaaca aatgaaacaa gaggagtgtc
    16201 aaatgacaaa agctaacttt ggtgtcgtag gtatggccgt aatgggtcgt aaccttgccc
    16261 ttaatattga atcacgtggt tacacagttg ctatctacaa ccgtagtaaa gaaaaaacgg
    16321 aagatgtgat tgcttgccat cctgaaaaga actttgtacc aagctatgac gttgaaagtt
    16381 ttgtaaactc aatcgaaaaa cctcgtcgta tcatgctgat ggttcaagct ggacctggta
    16441 cagatgctac tatccaagcc cttcttccac accttgacaa gggtgatatc ttgattgacg
    16501 gtggaaatac tttctacaaa gataccatcc gtcgtaatga agaattggca aactcaggta
    16561 tcaactttat cggtactgga gtttctggtg gtgaaaaagg tgcccttgaa ggtccttcta
    16621 tcatgcctgg tggacaaaaa gaggcctacg aattggttgc ggatgttctt gaagaaatct
    16681 cagctaaagc accagaagat ggcaaaccat gtgtgactta tatcggtcct gatggagctg
    16741 gtcactatgt gaaaatggtt cacaacggta tcgagtacgg tgatatgcaa ttgatcgcag
    16801 aaagctatga cttgatgcaa cacttgctag gcctttctgc agaggatatg gctgaaatct
    16861 ttactgagtg gaacaagggt gaattggata gctacttgat cgaaatcaca gctgatatct
    16921 tgagccgtaa agacgatgaa gaccaagatg gaccaatcgt agactacatc cttgatgctg
    16981 caggtaacaa gggaactggt aaatggacta gccaatcatc tcttgacctt ggtgtaccat
    17041 tgtcactgat tactgagtca gtgtttgcac gctacatttc aacttacaaa gaagaacgtg
    17101 tacatgctag caaggtgctt ccaaaaccag ctgccttcaa ctttgaagga gacaaggctg
    17161 aattgattga aaaaatccgt caagcccttt acttctcaaa aatcatttca tacgcacaag
    17221 gatttgctca attgcgtgta gcctctaaag aaaacaactg gaacttgcca tttgcagata
    17281 tcgcatctat ctggcgtgat ggctgtatca tccgttctcg tttcttgcaa aagattacag
    17341 atgcttacaa ccgcgatgca gatcttgcca accttctttt ggacgagtac ttcttggatg
    17401 ttactgctaa gtatcaacaa gcagtgcgtg atatcgtagc tcttgcggtt caagctggtg
    17461 tgccagtgcc aactttctca gcagctatta cttactttga tagctaccgt tcagctgacc
    17521 ttccggctaa cttgatccaa gcacaacgtg actactttgg tgctcacact taccaacgta
    17581 aagacaaaga aggaaccttc cactactctt ggtatgacga aaaataagta ggtcagccat
    17641 ggggaaacgg attttattac ttgagaaaga acgaaatcta gctcattttt taagtttgga
    17701 actccagaaa gagcagtatc gggttgatct ggtagaggag gggcaaaaag ccctctccat
    17761 ggctcttcag acagactatg atttgatgtt attgaacgtt aatctgggag atatgatggc
    17821 tcaggatttt gcagaaaaat tgagtcgaac taaacctgcc tcagtcatca tgattttaga
    17881 tcattgggaa gacttgcaag aagagctgga agttgttcag cgttttgcag tttcatacat
    17941 ttataagcca gtccttatcg aaaatctggt agcgcgtatt tcggcgatct tccgaggtcg
    18001 ggacttcatt gatcaacact gcagtctgat gaaagttcca aggacctacc gcaatcttag
    18061 gatagatgtt gaacatcaca cggtttatcg tggtgaagag atgattgctc tgacacgccg
    18121 tgagtatgac cttttggcga cacttatggg aagcaagaaa gtattgactc gtgagcaatt
    18181 gttggaaagt gtttggaagt atgaaagtgc gaccgagaca aatatcgtag atgtctatat
    18241 ccgctatcta cggagcaagc ttgatgttaa aggccaaaaa agctacatta aaactgtgcg
    18301 tggtgttgga tataccatgc aagaatagaa aagcagttgc agttgtgtaa ctgctttttt
    18361 gaggagtttt tatatattga catacaatct agtttttact acaatcgttt taggaggata
    18421 tataaaatga agcttttgaa aaaaatgatg caagtcgcat tagcagtctt tttctttggt
    18481 ttgctagcta caaatacggt atttgcgaat accacaggtg gccgatttgt tgataaggat
    18541 aatagaaaat attatgtaaa agatgatcat aaagcaatct attggcataa aatagacggt
    18601 aaaacttact attttggtga tattggagag atggttgtcg gttggcaata cttagaaatt
    18661 cctggaacag gttatcgtga taatttattc gataaccaac cagttaatga aattggcctt
    18721 caggagaagt ggtactattt tggacaagat ggtgctttgc tagaacaaac agataaacaa
    18781 gtactagagg caaaaacgtc tgaaaataca ggaaaagtat acggtgaaca atatcctcta
    18841 tctgctgaaa agagaactta ttattttgat aataattatg ctgtaaagac aggctggatt
    18901 tatgaagagg gtcattggta ttatttaaat aagctaggaa attttggcga tgattcttac
    18961 aatccactac caattggtga agttgctaag ggttggactc aagattttca tgttactatt
    19021 gacattgata gaagcaaacc tgctccatgg tactacctag atgcttcagg taagatgctt
    19081 acagattggc aaaaagtaaa cggaaaatgg tattattttg gctcctctgg ttctatggca
    19141 acaggttgga aatatgtacg aggcaaatgg tattacttag ataataaaaa tggtgatatg
    19201 aaaacaggat ggcaatacct tggtaacaag tggtactacc tccgttcatc aggagctatg
    19261 gtaactggct ggtatcaaga tggttcaact tggtactatt tagatgctga aaatggtgat
    19321 atgaaaatag gttggacaaa agtaaatgga aaatggtatt atctcaattc ctctggagca
    19381 atggttacag gtagtcaaac tatcgatggt aaagtttata acttcgcttc atctggtgag
    19441 tggatttaat gttggaggat atataaaatg aagcttttga aaaaaactat gcaagctgga
    19501 ctaacagtaa ttttcttcgg tttgctaggg accagtacag tatttgcaga tgattctgaa
    19561 ggatggcagt ttgtccaaga aaacggtaga acctattaca aaaaggggga cctaaaagaa
    19621 acctactgga gagtgataga tgggaagtac tattattttg attctctatc tggagagatg
    19681 gttgtcggct ggcaatatat cccgtttcca tctaaaggta gtacaattgg tccttaccca
    19741 aatggtatca gattaaaagg ttttccaaag tcagagtggt actacttcga taaaaatgga
    19801 gtgctacaag agtttgttgg ttggaaaaca ttagagatta aaactaaata cagtgttgga
    19861 agaaagtacg gggaaaaacg tgaagattca gaagataaag aagagaaacg ttattatacg
    19921 aactattact ttaatcaaaa tcattcttta gagacaggtt ggctttatga tcagtctaac
    19981 tggtattatc tagctaagac ggaaattaat ggagaaaact accttggtgg tgaaagacgt
    20041 gcggggtgga taaacgatga ttcgacttgg tactatttag atccaacaac tggtattatg
    20101 caaacaggtt ggcaatatct aggtaataag tggtactacc tccgttcatc aggattaatg
    20161 gcaactggct ggtatcagga aggtaccact tggtattatt tagaccagtc aaatggagat
    20221 atgaaaacag gctggcaata ccttggtaac aagtggtact accttcgttc atcaggagca
    20281 atggcaactg gttggtatca agatggttca acttggtact acctaaatgc aggtaatgga
    20341 gatatgaaga caggttggtt ccaggtcaat ggcaactggt actatgctta tagctcaggt
    20401 gctttggcag tgaatacgac cgtagatggc tattctgtca actataatgg cgaatgggtt
    20461 cggtaatgac agggcgctta taattatatt agaacactcg cttaatcaaa gaagtgactt
    20521 tgttgtactt gacctgacga ttggggagat taaaagcatg tgaaatctac acatgctttt
    20581 tcgttagtta tgagtgttga acttgactaa ccattgacaa aagataagag caattaaaat
    20641 aagatacaaa aatatcaatg ctaactgcac cgaaacgccc gatgctatgg atagggctaa
    20701 tagggagagc agggacgggg cgagaatgtc cactgtgctc agagcagagc ccaccgtccc
    20761 catcttgtca ctggggatat gggcaaagac agctgcttgt aggcgagggc tgagaatgcc
    20821 gataatcgta gaactgatga aactggtcat gaaaattacg atataggctt gacaaaggat
    20881 actagttatt agagtcaata gggaaatctc acagaaaacc aagaggtgcg tgaaacggat
    20941 atttttaaat agattactgc tggtcatatt gccaaggata gctccacaag acatgatgat
    21001 ggagagcagg gcgaggagtt gcccaatgct cagttgtgct agtgtctgtt ggatgaagcg
    21061 gagggcaatc agccgagggg acacatccaa gatggcaatc atactggtcg tcagaaacag
    21121 tagtatgacg gtatctttta acctcaggag cacagccagc gactgtttaa gatgttgaaa
    21181 atatttctta aaactcagtg ctgtatgtga catttcaatt cttttttcaa cctcatacaa
    21241 ggtatgtcga ataacataca ggcccaaaaa ggcaatgaca aaagtcagag tgttgataag
    21301 ggaaatagtt tgaatactta taacattgat aaggaatgcg ccagccagat tggcgacaat
    21361 acggacaacc ctcatcagag actgcctgaa ccccatggca tcatgcaggt cgtccttaat
    21421 tacactgatg tagagggcgt tcatcatgta ggcagacagg tagctcaagg tgtcagagat
    21481 gaggttgatg atgacaatca taatgactga aactaatgaa aagggcgaca agacgagaaa
    21541 tactgtcaaa atagcgtaga gcaggatttt gattttggca atccagagtg cgtgtttgac
    21601 tctattttct tgaaaatctc ctagaatccc gagaaagagt tgcgatagta ggggaaccat
    21661 ctctgaaatg gaaatcagta aaatcgctag cggagcgaaa ttgtactgag ccacataatt
    21721 gataatggcg aggtagaaaa tactgtcgcc gaatgatgaa aaacagctgt ttagcagcaa
    21781 ctgtctatat atgttgtttg tccaaaataa tttcatactt ttctccttat gtctgcacat
    21841 cgaatgggaa tttattattt aatagacacc aatatacccc atgcttccta ttccgaagtc
    21901 aaacaaatct gctttaccac tatctccttg aaacatcagt aaaccgtgat catctcgttc
    21961 agaacatgct aaaattacct gttggatttt tttcatcagt aatttgtttt gattgtagac
    22021 agtggtttgt agtaagctag ttattccgtg gcaatatcct gtcatcattt ttagatgatg
    22081 gttaaaaaca aattcttgat atttactaat aatatctttg tttccgtcac aatcgtacat
    22141 acaaagatat aagattattc cggaaattcc tttgcaccaa gaaagttgta aatttc
//
LOCUS       DAVLVZ010000028        22080 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000028 DAVLVZ010000000
VERSION     DAVLVZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.028"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..272)
                     /locus_tag="VYN77_001103"
     CDS             complement(<1..272)
                     /locus_tag="VYN77_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1558574.1"
                     /translation="MKSTKEEIQTIKTLLKDSRTAKYHKRLQIVLFRLMGKSYKEIIE
                     LLDCNQTTIWRNVKKYEEFGLDSLLQETRGGRNHAYMTVEEEKAFLA"
     gene            complement(656..1852)
                     /gene="tuf"
                     /locus_tag="VYN77_001104"
     CDS             complement(656..1852)
                     /gene="tuf"
                     /locus_tag="VYN77_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1558575.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2763..3632)
                     /locus_tag="VYN77_001105"
     CDS             complement(2763..3632)
                     /locus_tag="VYN77_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1558576.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3855..4376)
                     /locus_tag="VYN77_001106"
     CDS             complement(3855..4376)
                     /locus_tag="VYN77_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1558577.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDKK"
     gene            complement(4334..5440)
                     /locus_tag="VYN77_001107"
     CDS             complement(4334..5440)
                     /locus_tag="VYN77_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1558578.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5551..5805)
                     /locus_tag="VYN77_001108"
     CDS             complement(5551..5805)
                     /locus_tag="VYN77_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558579.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(6163..6609)
                     /locus_tag="VYN77_001109"
     CDS             complement(6163..6609)
                     /locus_tag="VYN77_001109"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1558580.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDVLREVPTIFECLECFFKTR"
     gene            complement(6682..7007)
                     /locus_tag="VYN77_001110"
                     /pseudo
     CDS             complement(6682..7007)
                     /locus_tag="VYN77_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7241..8959
                     /locus_tag="VYN77_001111"
     CDS             7241..8959
                     /locus_tag="VYN77_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1558581.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            9070..9417
                     /locus_tag="VYN77_001112"
     CDS             9070..9417
                     /locus_tag="VYN77_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1558582.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9696..10532)
                     /locus_tag="VYN77_001113"
     CDS             complement(9696..10532)
                     /locus_tag="VYN77_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1558583.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10545..11174)
                     /locus_tag="VYN77_001114"
     CDS             complement(10545..11174)
                     /locus_tag="VYN77_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1558584.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(11184..11825)
                     /locus_tag="VYN77_001115"
     CDS             complement(11184..11825)
                     /locus_tag="VYN77_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1558585.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11971..13200)
                     /locus_tag="VYN77_001116"
     CDS             complement(11971..13200)
                     /locus_tag="VYN77_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1558586.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILELQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEYYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(13190..14356)
                     /locus_tag="VYN77_001117"
     CDS             complement(13190..14356)
                     /locus_tag="VYN77_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1558587.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14500..15513
                     /locus_tag="VYN77_001118"
     CDS             14500..15513
                     /locus_tag="VYN77_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1558588.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15558..15977)
                     /locus_tag="VYN77_001119"
     CDS             complement(15558..15977)
                     /locus_tag="VYN77_001119"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1558589.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15988..17394)
                     /gene="atpD"
                     /locus_tag="VYN77_001120"
     CDS             complement(15988..17394)
                     /gene="atpD"
                     /locus_tag="VYN77_001120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1558590.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17480..18358)
                     /locus_tag="VYN77_001121"
     CDS             complement(17480..18358)
                     /locus_tag="VYN77_001121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1558591.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLSDQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(18374..19879)
                     /locus_tag="VYN77_001122"
     CDS             complement(18374..19879)
                     /locus_tag="VYN77_001122"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1558592.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19894..20430)
                     /locus_tag="VYN77_001123"
     CDS             complement(19894..20430)
                     /locus_tag="VYN77_001123"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1558593.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20430..20924)
                     /gene="atpF"
                     /locus_tag="VYN77_001124"
     CDS             complement(20430..20924)
                     /gene="atpF"
                     /locus_tag="VYN77_001124"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1558594.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20938..21654)
                     /gene="atpB"
                     /locus_tag="VYN77_001125"
     CDS             complement(20938..21654)
                     /gene="atpB"
                     /locus_tag="VYN77_001125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1558595.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21689..21889)
                     /locus_tag="VYN77_001126"
     CDS             complement(21689..21889)
                     /locus_tag="VYN77_001126"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1558596.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(21964..>22080)
                     /locus_tag="VYN77_001127"
     CDS             complement(21964..>22080)
                     /locus_tag="VYN77_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1558597.1"
                     /translation="NNYPKKYLDYKSPREDFWMANLNLKFSKMEIIFIKRFQ"
BASE COUNT         6592 a         4743 c         4007 g         6738 t
ORIGIN      
        1 gcaagaaagg ctttctcttc ctcaactgtc atatatgcat ggttacgacc accacgtgtt
       61 tcttgaagga gagagtcgag tccgaactcc tcatattttt ttacatttcg ccaaatcgtt
      121 gtttgattac agtctaaaag ctctataatc tctttataag atttgcccat cagacgaaat
      181 agaacgattt gaaggcgttt atgatattta gctgtacgag agtcttttaa aagtgttttg
      241 atggtttgga tttcttcttt agttgatttc atattactat tgtataatgc tttttgattt
      301 gagtctagta ttatactctt cgaaaatctc ttcaaaccgc gtcagcttcg ccttgtcgta
      361 tagatgttac tgacttcgtc agttctatct acaacctaaa aacagtgttt tgagcagtct
      421 gcagctaact ttctataaca aaatgaaata agaacaagac aaatcaatca agataatcaa
      481 atcgatttct aacaatgttt tagaagtaga tgtgtactat tctagtttca atatattata
      541 gtttgctctt tgattttcat tgagtattag ttcaatatct aaaaaaagaa ccttgccaag
      601 caagattctt ttagtgtctg acttaaataa ttgttcttct gggaactaaa tcgaattaag
      661 cttcgatttc tgtaaccata cctgaaccaa cagtacgtcc accctcacgg atagagaatg
      721 tagtaccttg ttctacggcg attgggtgaa tcaactcaac gtcgattgtc acgttatcac
      781 caggcattac catttcagta cctgctggaa gttcgattga acctgtaacg tcagtagtac
      841 ggaagtagaa ttgtggacgg tagttgttga agaatggagt gtgacgtcca ccttcttctt
      901 tagtaaggat gtagacttca cctttgaatt tagtgtgtgg gttgattgaa cctggtttag
      961 cgataacttg tccacgttcg atttcatcac gttgaacacc acggagaagg acacctacgt
     1021 tatctccagc aagaccttcg tcaagttgtt tacggaacat ttcaacacca gtaacaactg
     1081 ctttttgagt ttcttctttg ataccaacaa tttcgatttc gtcgttgact ttaacgatac
     1141 cacggtcgat acgtcctgaa gcaactgtac cacgtccagt gattgagaat acgtcctcga
     1201 ctggaagaag caatggtttg tcagtgtcac gttctggttc tgggatatac tcatcaactg
     1261 tgttcatcaa ttccataacg atgtcttcgt atttagagtc accttcaaga gctttaagtg
     1321 ctgaaccttg gataactgga agatcgtcac ctgggaagtc gtattctgac aataggtcac
     1381 ggatttccat ttcaaccaat tcaagcaatt cttcgtcgtc aaccaagtca actttgttca
     1441 tgaagacgat aaggtgttta acaccaacct gacgtgaaag aaggatgtgc tcacgagttt
     1501 gtggcattgg tccgtcagtt gaagctacta caaggatagc tccgtccatt tgagcagcac
     1561 cagtgatcat gtttttaacg tagtccgcgt gtcctggagc gtcgatatga gcgtagtgac
     1621 gtttttcagt ttcgtactca acgtgcgcag tgttgatagt gataccgcgt tcgcgttctt
     1681 ctggagcagc atcgatagac gcatagtctt taggttggtt aactgatgaa ggcaagcgac
     1741 gtgccaaaac agttgtgata gctgcagtta gggtagtttt accgtggtca acgtgtccga
     1801 tagtaccaat gttaacgtgt ggtttactac gatcgtattt ttcttttgcc atttgagtaa
     1861 aagcctccaa taaaatatat tttatagata gacagtaggc aatacagtct aactttcctt
     1921 actattttat caaatttcag ctaaattgca agtgttttac aacgtttttg tgaattattc
     1981 ttggtaaaaa tttaactaat taatagaaag tactaaatag acttatcctt caaggagttt
     2041 tcagcattat ccatccactc acttgctgaa aatgtaaaca gtctctggtt tatatagtaa
     2101 aatgaaacaa gaatagggca aatcgatcag gacagtcaaa tcgatttcta acaatgtttt
     2161 agaagtagat gtgtactatt ctagtttcaa tctactatac aacctcaaaa ctgtgttttg
     2221 aacaacctgc ggctagcttc ttagtttgcc ctttgatttt cattgagtat aaaattgcag
     2281 ataaaacgat aacaatcata ggactcagca gagtcaatgt aggtcgaaga atagcccccc
     2341 taatcgctta ttttatagca aaatgaaata gaaactgaac attttgttga ggaaggtaaa
     2401 tcaattcctc acaatgtttg agaaagaagg ggggactatt ccaacttcaa cccactataa
     2461 agcagactta caagatagag aacccatctc cgataatata tagtagaatg aaaactgcac
     2521 aatttgattg gagaattcaa accaatttct aacaatgttt tagaaacaag ggtctattat
     2581 ttcaactgca acctactata gcccattgaa gctggaatag cccactgaga cttctaaacc
     2641 actgctggag aggagtttgt ttagagtttc tttcatttcc ctaaaaaatc aaaaagagag
     2701 aaccagtctg atcctctctt acttatagat ggtgctcact caaatgttaa aggagctatt
     2761 tcttagagat aaaggaattt gaatacagct actgccgcaa ttgctgctgc gataggtgct
     2821 actactggta cccaagaata ccaccatttt gaatcgcctt tatgctcacc aagaactgat
     2881 tttggaagga aagcatgaag gagacgtggt cccaagtcac gggctgggtt caaggcaggt
     2941 cctgtaggtc ctccaagtga tgttaccaaa gccataacga ggaatccaag tgccaagtga
     3001 gccacagaaa gtcctgaagc agtgtgtgga gccacctgtg ctttaatagc caagtcagaa
     3061 aaatcaactg tttgtcctgc ttctattgcc ttttgtttca tgaattgaag cacttcagca
     3121 ccaaagaagt ttttagtcaa accaagagct gcaaagaaca aaacaaatga accaacaaac
     3181 tcattaatca aaccattgac agttgctgca tagcgacttt cttttgtacc atggtcaata
     3241 cttgaaatag ttgagaaagt tcccaagatg ttatttggtt tttcagtttt caagtagaat
     3301 ggacggtatg ttgccacaac taaggcttgg ccaaagatag cccccaagac ttgcgcgata
     3361 atgtaaggta ccacttgtgc ccaagggaaa agaccgctaa ctgcaagccc tagagtgaaa
     3421 gcagggttga tgtgattccc agatacgtta ccaaacatca aggctgggat cataaccccc
     3481 ataccataac caacagcgat gacgatccag ccactttggt gacctttcgt acctttaagt
     3541 tcaacgttgg caactgcacc attcccaaga atgatcaaaa tggcagttcc caaaaattca
     3601 gtggcatact tcagtgccca tgtgaaatcc atttgctaga ttctccttaa aattttttag
     3661 atattcctta ttttatcaat ttttacccta tttagcaact gatttctgga aatattttta
     3721 aaaaaatagg attcatagtt tcccagttca acgtacttgt aagcgttttt attcttcacg
     3781 aaagacctca ttctcaaaac caggaaaaag cccacatctg tgatcttttc cttgttccta
     3841 gattctaaaa tctattattt tttatcttct tttttcttga agaatcctaa tcctagacca
     3901 gctaacaagg tcatgatacc agcactagcc aaggtagcat tagcttctgt acctgtgttt
     3961 ggcaattcct ctctcttacc tgtctcataa gtcggaactt ctgggtctgg attcactgga
     4021 gtttcagttt ttggagtacc tggttctgga gttggtttat ctggtgttga taaacggtca
     4081 taccttaccg ttatttcttt atcactagag tctgacgtaa cttcttgtga ttcaactgtt
     4141 ggaatatctg gatctttgta cttgtcaatc ttaccagata taacctcgtc ccagtttcct
     4201 gttgtccatt caccgtaggt tacaactccc gtgaccttgt tctcagtttt tgtacggctt
     4261 aaggttacag gttgaacaac atcttctttt acattttggt tcgtaacttt atcaacgtaa
     4321 tgaatgatac gcgttatagt cttcgtctca gtagaggttg ctgttttggg aaccactgtt
     4381 tcctcaacat tctcacggta gtaatagtca actgttgcac cgtcttctgg tacgcatttg
     4441 caggagttgc taccaaggtg tatgttgttt tccttgtgat aactcggtct tctttgtcct
     4501 cagttgttgt tttcccttca atagtttttg attctgtggt atactcagaa cctatcgcta
     4561 aatcagcttt tataacagac tctaccaact tctcttgcct accttcttta tagtaattgg
     4621 ctgttactgt agcagtggtt ggcgcttcgc tttctctata aactaaggtc actgttctac
     4681 cttcgcttac aatattccca gttaaacttg cagaatttgt atctgcttct ttaaaagtat
     4741 aatattttcc gtcagtagta gtcatgctac tgagtttttt atctgtgaca ttatagctgg
     4801 taccaatcag ttgtttttta ttggtaatgt aggttccgtc actttctttt tctccaattc
     4861 cagtatcatt tttcaatgat agcaaacgcc cttgttcatc aacatagcga actttcacat
     4921 tttctgagat tagttctgcc aattctgagg tttttttctt tttcttgatt tcttcggtta
     4981 ttttcccttt ctcttcttcg ggaatattta gttttggaat gattttttca acaacggttc
     5041 gtgatggttc cacagtatct tggatgactg aaaagtcagc tagaattggg agattatact
     5101 gaacacggtg actttgagtg tttactccta ctctttcatt attctctgaa aatacttgta
     5161 cggtataaga aacaacatct tttcctaata gaacatcccc agtagagaaa tagccgcctt
     5221 ttcctagttt gctatctcca gagcccactt ctttcctaat cttatcagat agttttttac
     5281 cagtcagtac attcgttcgc acaatccctt tgtctacccc tacaaagtgg gagaactttt
     5341 tgaactcttc agaaccagat ctagcccaac cattattaag ggcatttgct tttgtatttg
     5401 tattctctct caaaagtttg gcgattagaa ttatattcat cggcacttag agttgctgct
     5461 atatctgact cttgaatacc aacttcctta ctaccatttc tagcggcagt atatgtgtat
     5521 ttatctgttt ctacttctag ctgattatcc ttaacatgat aggtaatttt ctcctggtat
     5581 aagagtcttc agtagtttat atactctgtc atcatcttta gctttagaag cagctgagtt
     5641 tagccaagtt ggggtattag gagtatcttt atcagcctgt ttcaagacat tattcaagtc
     5701 atttgctagc ctattggtta tcctcactgt gaaagttttc ttccagtctg aaccactttt
     5761 attagactta gatatgctca tgaaatcctg gtcaacattc aacacaatat cgccttgttt
     5821 tttagcatct tccgttgctt ctgcttcaaa ttgatcatgg agtttttgtt gctctggtgt
     5881 atagtttttt tatttacacg atttaatgta ctatttttta ttttagtatt aagaactcca
     5941 ttaatacgat ttttaaactc tgcttcaaaa aatcccttaa tttgttggtg cgcaagttca
     6001 acattcaaac ctttcttata caagtcttca ttctgataca agtcttcatt ctgaacgatt
     6061 tcggctacac ggtgaatggt ttgattgtat accgtcttct cttaaccagc agtatcctta
     6121 gttttaatga ctactcgata ggttccttta gaaccgttat agtcatctag ttttaaaaaa
     6181 gcattccaaa cattcaaaaa tagttggaac ttcccttaat acatccgtca ctttcttttt
     6241 caagatagcc caagcttgct caataggatt caaatccggt gaataaggtg gtagaggtaa
     6301 gaaaaagtgt ttatcttgga tgcagagttc atccaaaatg ttcttgggat gaaaaccagc
     6361 attgcccatg acaataacat gaggtgtctt caaagcaggt aggagttgcg ttttgaacca
     6421 ctccacaaag aaatcgcttg tcatgctttt cttgtaaatc atgggagcta taaactctcc
     6481 gtctacttgt cctgcaacaa ttgaagttcg ctcaaaacga cgtccgctaa tcttttcata
     6541 gactttctcc cctctaggag cccctgcata aggacgatag agatagcggt cgattcctgt
     6601 ttcatcaata taaacaggtg ctaggtgctt taaactatta aaattcttaa gaaataaggc
     6661 tacttttctg ggtcttgttc cttaaagctc gtcatctttt tttaaagtga ccttaatctg
     6721 ctttaaagct gcccatactg aaggaacagc acaatcaaaa tgtgccgcaa tttcccgtaa
     6781 aaaagcatct ggatgagcct ctacaaaggc tttcaattcc tctaaaggaa tctttcggtt
     6841 tttgacgact cgctttttcc tctctatgtg ttcttgttca cgtctttctt ttcccacgtg
     6901 aagagagttc tgacgccaac accaaaaaac ttggctgcct cgacatggct gtgcccctct
     6961 ttgatgtaat ctaatgctcg ctgtttaaaa tcagtactat atgccatagc tttattataa
     7021 tatgcttgtt taaaactaag taactatatc aatgttttgt tatgagaaga cttacttttt
     7081 ttactgaaat tgaatttttc ctagcctcaa catcatataa ccccaaaatt caatctgacc
     7141 tggtttgaag agatgttcga agcagataag gttttactaa aactaaaaaa aatgatataa
     7201 ttaaactgat taaatgtttt aaaaataaag gagaatttga atgtcttacc aagaaaatta
     7261 ccagaaatgg gttgattttg tggagcttcc tgactacctt cgtcaagatt tggaaaatat
     7321 ggacgaaaaa actaaggaag atgccttcta tacaaatctt gaatttggta ctgcaggtat
     7381 gcgtggcttg gttggtgctg gtacaaaccg tatcaacatc tacgttgttc gccaagctac
     7441 tgaaggattg gctcgtttga ttgagtcaaa aggtggaaac gagaaagaac gcggtgtagc
     7501 aattgcctac gatagccgtc acttctcacc tgagtttgcc tttgaatctg cggcagttct
     7561 tgctaaacac ggcatcaaat cttacgtatt tgaaagcctt cgtccgactc cagaactatc
     7621 atttgcagtt cgtcatctca actgtttcgc aggtatcatg gtcacagcca gccacaaccc
     7681 tgctccattt aacggttaca aggtttacgg tgaagacggt ggacaaatgc ctccacacga
     7741 tgcggacgct ttgactactt atatccgtgc aatcgaaaac ccatttgcag ttgaagttgc
     7801 tgatgtggaa actgaaaaag cttctggctt gattgaagtt atcggcgaag ctgttgacat
     7861 agaatacctt aaagaggtta aggacataaa catcaaccca gccttgattg aagaatttgg
     7921 taaagacatg aagattgtct acacaccact tcatggtact ggtgaaatgt tggctcgtcg
     7981 tgctcttgcc caagcaggat ttgactctgt tcaagttgtt gaagcgcaag caactgctga
     8041 cccagacttt tcaactgtaa catctccaaa cccagaaagc caagcagcct ttgcccttgc
     8101 tgaagaactt ggtcgtcaag ttggtgcaga tgttcttgtc gcaactgacc cagacgctga
     8161 ccgtgttggt gttgaagttc ttcaaaaaga tggtagctac ctcaaccttt caggtaacca
     8221 aatcggtgct atcatggcta aatacatctt ggaagctcac aaaaacgctg gaactcttcc
     8281 tgaaaatgcc gctctctgca aatctatcgt ttcaactgac ttggtaacga agattgctga
     8341 aagctacggc gcaactatgt tcaacgtctt gacaggtttc aaatttatcg ctgaaaaaat
     8401 ccaagaattc gaagaaaaac acaaccacac ttacatgatg ggatttgaag aaagcttcgg
     8461 ttacttgatt aaaccatttg tacgtgataa agatgccatc caagccgttc ttgtagttgc
     8521 tgaacttgct gcctactacc gttctcgtgg tttgacactt gctgacggta tcgaagaaat
     8581 ctataaagaa tatggctact acgcagaaaa aacaatctct gttactcttt caggtgtcga
     8641 tggtgctgaa caaatcaaag cgattatggc taaattccgc aacaatgctc caacagaatg
     8701 gaacgcaaca gctatcactg tcgtagaaga cttcaaggca caaactgcta ctgtcgctga
     8761 cggtactgtt acaaacttga caactcctcc aagtgatgtg ttgaaataca cacttgctga
     8821 cggttcatgg attgccgttc gcccttcagg tacagaacca aaaatcaagt tctacattgc
     8881 agttgtaggg gaaaccaacg aagaatcaca agctaagatt gctaacatcg aagcagaaat
     8941 caatgcattt gtaaaataaa gacctataaa agatgagacc aaccaacctc atcttttttc
     9001 gtatcaaatc taagcaattt tagaaacttt atggcatact agacttatca atgaaagcga
     9061 ggtaatctca tggaacaatt tttagataac atcaaagacc ttgaagtcac tacagttgtg
     9121 cgtgcgcaag aagctcttga taaaaaagaa actgcaacct tctttatcgg tcgcaaaact
     9181 tgcccttact gccgtaaatt tgcaggtaca ttgtcaggtg tcgtagctga aaccaaagct
     9241 cacatttact tcatcaatag tgaagaacca agccaactca atgatttgca agcattccgc
     9301 tcacgctatg gaatcccaac tgtaccaggt tttgttcata ttacagatgg acaaatcaac
     9361 gtccgttgcg actcttcaat gtcagcacaa gaaatcaaag atttcgcagg attgtaaaaa
     9421 atcagctaac aagtcttaaa atcacaaaaa cgcataatgt aaggtgcaaa aaccttgata
     9481 ttatgcgttt tattgttcct aaaacagtta catcagtatt tcattacaga acattttaat
     9541 tttggaagtc aaatcaattt ttaacaatgt tttagaaatc acagtgggct attccatatt
     9601 caattatcca tattcaatcg ctattatcgt tttcctagat tacaaaaaaa actacgtttt
     9661 gctaatcaaa acgcagtttc agccactatt ttatcttact gtccttcttt tacttctttg
     9721 gttgctacat cttctccaaa ccatttttgg ctgatttctt ggaacttgcc gtccttgtaa
     9781 agactagaaa aagcttcatt tatcttctta accaagtttg tatcttcctt acgggctcca
     9841 accgcaaaag cttctgtttc tagtccaact gtaaagacat tataatcgtt taaaacacct
     9901 tctgcttcta aataatagtt tgcatagaca cggtcaatca atagaccatc aattcgatcg
     9961 tttttcaaat caatcaaggc ttcattaaag gtttggtatt gattcgcttc cttattagcg
    10021 acaatattct tcaaaatttc tggatttgct tcaaagtccg cataaccaga tgaaccagct
    10081 tgagctccta atgtctttcc agtcatatcc tttgcagtcg tgataccaga tgatttcttc
    10141 gtaaccaata cctgctcatt cttcatatat gagttactga aagccacctt ttcacggcgt
    10201 tcgtctgtag cggaatagcc attccaaatc agatcaatcg ttccttttgt caattcagct
    10261 tctttcaaat cccaatcaat cggttgccaa tttaccgtga ttccgtattt ttcaaaaaca
    10321 gctgtagcta aatcaatatc aaatcctgca taagaaccat ctttctgagc aaatcccatt
    10381 ggaacaaaag tactatcaaa tccaatagta atagacttgt tagactggta ctttgaccaa
    10441 ttatctccac tagtttcgct agaatttttc ccacaagcta ctaagaacaa agcagtcatc
    10501 agactgacta atacaagcat ccattttttc atccattttt cctcctattt aggttctact
    10561 ttcaataata catctgcgat attttcagca aactgcaaat catgggtaac cacaatctgg
    10621 gtcatcccaa gttccctatt ttgcaagatt agcttctcca cttccaaacg taattctgga
    10681 tccagggcag aagttggttc atcgtagcca atgatttctg ggtcaatcat catagcacgc
    10741 gccaaagcca cccgctgctt ttgcccacca gatagtgaga aaggataggc ctctgcgtgt
    10801 cctcctagtc ctaactgttc caagagtcca ctcgccttct tctcagcctc ttcctgcttc
    10861 attcccatgg tcttcacagg cgataaagtc aaattttcca gaactgatag atgaggaaat
    10921 agttgaaaat cttggaagac aaatcccagt agattgcgct tctgcaattc atccagctct
    10981 aaaggttgtc cattataaaa gatttgccct gaatcaatgg tttcaagacc tgcaagcata
    11041 cgtaaaagag ttgtcttacc tccaccagaa ggtccaacga tagccaggat ttgcttttca
    11101 ggaatactta gactgaaatt agacaggatt tgtttgtctc caaagacttt attgatattt
    11161 cgtaattcta acatggcagc ctcctatcta taataactat acttcttctc aacttttttg
    11221 gaaataattg tcacaatccc aatcaaaatc aaataaatgg ctcctgccaa gaacatagga
    11281 actagactag catcgcggtt agcagctgtt cgactagcca agataaggtc tgaaattccg
    11341 agagcataga ccaaagaagt atccttgacc aaactcataa cttcattaaa gacactagga
    11401 agaacgatct tggtcacttg gggcaagata atatagcgca ctctgtcaaa agggctaaac
    11461 ttcaagacct tggcggcctc atactgtcct cttggaatag tgtcaattcc cccacggaaa
    11521 atttctgcaa agtaagctgc atagttgaga acaaaggcaa taatagctgc aggaaggcgg
    11581 tctaaacgaa tcccaatact tgggagcaca taatagataa aaatcagttg caagagtaag
    11641 ggggttcctc gcataaccca gatgtaaatg ttaatcagat aatggagggg cttccaatgg
    11701 acttgcaagg caaaggcaat caaaacgccc aagggaatcg aaaatagcaa gaccagtgca
    11761 aagacctgta cagtcgtgct cgcaccattc agtaaactcg gtaatatctc aaataaataa
    11821 gacatactgc acctcctaaa agtaattgtt cctattatag catgaataaa caagattacc
    11881 aagtattttt gtaaaaagat tctaccatat acaaataaaa accgtagcta tcaaaagaga
    11941 taaactacag ttctatatgt ttaaacattc ttacaatctc tcaaacagtt cttgcatttg
    12001 cacatcatct ggaaccagtt ttaagtaagt gtgagcatgg acttttgctt cttcaaaatg
    12061 tcccaattca cgcaagagat agatatagtg ttccagaaat tctggattgt ccttcaaatc
    12121 tcctgtcaac tcttgataat actcataagc agtatccaaa tcgtccattt cttgataaga
    12181 acgagcaatc atccacttgg tcaaaagatt ttctggctcc tcactctgca attctagaat
    12241 atcctcataa cgctcctgct ccagataaat agtggctaaa cgaagcaaga tttcttctgt
    12301 atcctcagcg tcttcttttg cagtaaggag ataattttct gcaccactag catcatgcaa
    12361 ttcataagaa aattgtgaag cagctagcaa gaggcgagtt tcaaagggat ttttctctaa
    12421 tccttgctta gcgatacgca gggcttcttg aacttgatgt tccttatgta aagcctgact
    12481 gtacccatac tcatagcctt caaagtcagg agaaatggta tcaagctgct taaagtagag
    12541 ggtggctttt tgatattctt cttgatcaaa ataaagactg gccaactcaa aagctgttaa
    12601 gtcatcgtat tctaactcca gggctttttc taaaaactca gtagccgttt caaatttccc
    12661 taactgagca taggcaaagc caattcgttg ataggtggaa atgcccgttt gctcataaat
    12721 cgagcgatta tctaactggg cataggcttg aatagccgct tggtaatttt ccaactcact
    12781 atccaactct gccaaaccca atatcaagag agaatcctct gagtaggtca aggcctccaa
    12841 taatttctca cgtgccacat ctgtcaaacc ttccagctgg taaaggtctg ccttcagagc
    12901 caaagacgag acataccagt cactgtcagc ttggatttcc tcaagatagg taaaggcttc
    12961 ttctatttga ccatcctcgc tagcaattgc agctagatta agatgaacct ctggaaaatc
    13021 ctctacaatt ttcaggtaaa tttccttggc ctgaggatag aaaccaatcc cttcaagata
    13081 agttgccaat tcatacagaa gatcacttga atcattttct aaagctttgg cgaaataatg
    13141 ctcagcctta gttaaatctt gctcctccaa agcctgtaac atctgttgac tattgttcac
    13201 tcttgacttc ctcaccctct aattcatata gaccactgac taccttatac cattcgaaaa
    13261 tggctgaaat gacaacctta gcagaggcat aaaccggaat accaagtaaa actccccaga
    13321 taccaaacat agatcctgaa gttaacaaaa caaagagaac attaataggg tggatgttta
    13381 attgacttcc caaaatcaat ggagagacaa aacggccttc aatagtttgt tctacgataa
    13441 agacaatcac tactttcaaa agcatgactg gaccagcaat caaacccaat actagagcag
    13501 gaagcatggc tagaaagcta ccaagataag ggaccagatt taaaatacca gcagtaaccc
    13561 ccagcgtaac cgcatagcgt agaccaataa tcttgaagaa gatgataaac attactgcta
    13621 caataatagc cactgtcact tgccctcgaa catagttgga caactgttga ttcacatctg
    13681 ataaaacttg tccaacaggt tccttcaatt ttcttggaat gaattgggtc aaatagttac
    13741 gcaagccttt cccatcacgc aagagataaa agagcatgaa aggaacgata atcaaggcaa
    13801 caatcacttg agaagccccg ctaataaagg cactcaccca gttgactgcc tgagatgaaa
    13861 ccttacttgc caaaactgta gcctggctag aaaaattggt caaaacttgc tctaattgag
    13921 gtctgaaatc atctggcagg tgctgggcta ccaatccatt aacaatccta tctatatctt
    13981 ctaagtaaac aggaacgttt cttgcaaagg tcaaaacctg acgttgcaga tttggaatgg
    14041 cgactgccaa gccccaaatg ataaagagag cgatgataac aaagacaata gtgatagcta
    14101 taacacgatt aaccttatgc ttctccatcc aatcaacaat aggattcaac aaataatata
    14161 acaaaccaga caaaatgact ggcaacatca caactgctaa aaagtctaaa acaggtgaaa
    14221 atagaaaact aatcttactt aaaataaaaa gattcagtcc caataataag gttactaaaa
    14281 ataccgtaat tgccttgtta tctaaaaacc acttgaaaaa ccaagatagg ctaaaatgtt
    14341 tctctttttg ctccataaat acttcctttc tattgttctc tcattatacc atttttgaaa
    14401 caaaataact cttttttaaa gtgcttacat ctaattggat atctttaatt tacttgtttt
    14461 tgtgatataa tagtcttatc tttacacaga ggtatagtaa tgaaagaaac tgtttatttt
    14521 ggaacttata cacgtcgtac ttctcaaggg atttacaagg cagactttga tacagaaact
    14581 ggtcagcttt caaatctaga actttttgca gctgagccaa gtccaaccta ccttgccttt
    14641 gaccagcacc aacatttata cactgttggt agccaagacg ataagggggg aattgcagcc
    14701 tatcaaactg acgggactgt gttaaatcat gttgttgaag aaggagctcc ccactgttat
    14761 gttgctgtcg atgaaaagcg tgatttggtt tacgcagcta actatcacaa gggacaagtc
    14821 cttgtttata aacgccagga agatggtagt cttctactta gtgatatgga tcaacacagt
    14881 ggccaaggtc cacatgaaaa tcaagcttcc ccccatgttc actatacaga tttaacacct
    14941 gaccactatc tagtgacctg cgacttggga actgaccaag tcatcaccta tgacctcgat
    15001 caagaaggaa aattatctaa gctctatacc tatcacagca agccaggagc aggctcacgc
    15061 catatcattt tccataacca ctataaaatc gcttatctca tttgtgaact caatagtact
    15121 atcgaagttt taatctacga tggcgttggc gaatttgaac gtatgcaggt catttcaact
    15181 ttaccggaag cttacgaagg ctttaatgga accgctgcta ttcatctctc taaagacggt
    15241 aaatacctct acgcttctaa ccgtggccat gattctatcg cagtatatac catccttgcg
    15301 gacggtagct tagagttatt agaaatcgtt ccaacgcatg gtcagactcc acgtgatttt
    15361 gatttgacac ccgaccaaaa atttctcatt gttgtccatc aagactctga caatgcaact
    15421 gtctttaaac gtaattgtga caatggtcgt ctagcagaac tctccaacga cttccatgtt
    15481 cccgaagcag tctgcatccg ttttgctcct taaaacacca taaaaaatga acttggtatt
    15541 ttcaagttca ttttttctta tagtctattt ccgacattaa tacggttaat agcacgttgc
    15601 aaagcaatct tagcacgacg ttcttggtca atcaaatgtt tgtcttgtgc ttcttcaatt
    15661 gcacgttctg cacgaagttt ggcacgttct gcacgactga tatcgatatc acgagcacgt
    15721 tctgcagagt cagcgacgat tgtgatcata tcattggcaa tttcaataac gccaccgttt
    15781 actgcaatcc agttcacgtg atctttatca tcgatacgtt ttacctttac ttcatcaact
    15841 gctaaaaccg caatcatatt ttcatgtcgt ggcaagatcc ccatctcacc atccagagtt
    15901 cgaaccgata catagctggc atggtgatca tagacgagac catctggtgt cacgatctgg
    15961 acagttaact gagccataga tcacctctta aaatcccatt ttttcagctt ttgcaatcac
    16021 atcttcgata gaacctacac cacggaaggc atcttctggc aagtggtcgt atttaccatc
    16081 aaggatttcc ttaaagccac gtacagtttc agcaactgga acataagaac ctggctgacc
    16141 agtaaattgt tccgcaacgt tgaagttttg tgacaagaag aactggatac gacgggcgcg
    16201 agcaaccaag gtcttttctt catcagaaag ctcatccata ccaaggatag caatgatatc
    16261 ttgcaattca tggtaacgtt gaaggacacg ttttacttca gcagcaactg catagtgctc
    16321 ttctccaacg atttcaggtg ccaaggcacg tgagcttgaa gcaagtgggt caacggctgg
    16381 gtagataccc aattgtacca acttacgttc caagtttgtt gttgaatcca agtgagcgaa
    16441 ggctgttgct ggcgctgggt cagtatagtc atccgctggc acatagatag cctggataga
    16501 ggttacagaa cccttcttgg ttgatgtgat acgttcttgc aattgaccca tttccgtagc
    16561 aagtgttggt tggtaaccaa cggctgatgg catacgaccc aaaagggcag atacttctga
    16621 accagcctga gtgaaacgga aaatattatc gataaagaga agcacgtctt ggccttccac
    16681 atcacggaag tattcagcga ttgtcaaacc agtaagggca acacgcatac gtgctcctgg
    16741 tggctcattc atctgaccaa agaccatggc tgttttctcg ataacgcctg attctttcat
    16801 ttcccagtaa aggtcattcc cctcacgagt acgttcccca acaccagcaa atactgaaat
    16861 accaccgtgc tcttgggcaa tgttgtgaat caattcttgg attaagacag ttttaccaac
    16921 tccggcacca ccgaaaagtc caactttacc acctttaagg taaggggcaa gaaggtcaat
    16981 aaccttgatc cctgtttcaa ggatttcaga agaggtagac aactcatcaa aagttggagc
    17041 ttttttatga attggctgac gctctgcgtc ttctgtaaaa ggagcttcca agtcaatggt
    17101 atctcccaaa acgttgaaga cacgtcccaa agtttcttta cctactggta cagagattgg
    17161 acgacctgtg tccaatactt ccattccacg agtcaaccca tctgttgatt ccatggcgat
    17221 agtacgaacc ataccatctc ctaactccaa ggctacttca aggacgattt ttgtttttct
    17281 ttcgtcattt ttgtagacga caagtgcatt gttaatctca ggaagttttt cccctgctgc
    17341 aaacaaaacg tctacaacgg gaccgataac ctgagcaatt ttacctgaac tcatctcctt
    17401 ctcctattct atataagata ctgtcggttc ctagctcaac taggtcctaa gttcattttc
    17461 atacgagctg gactagagcc tattctaagg cactagcacc tgctacgatt tctgtaattt
    17521 cttgtgtaat cgccgcctgt ctggcacggt tatactgaat tgtcaaatca ttgatgactt
    17581 tcttagcatt atctgtcgct gtttgcatgg ctgtcatgcc cgcagcattc tcagctgtct
    17641 tggcatcgat aatggcaccg taaatcatac tttctgcaaa ctgaggcaac aactgctcca
    17701 gaatttcttc tcggctggtt tccaattcaa aagtcaagct gtactcttca tccgcttcat
    17761 ttggatccaa gtcaacaatc ggaagcattt gttccacacg catttgactg gttagcgtat
    17821 tgacatggtg gttgtagcaa acataaagct catcaaagag ttcattttgg tacatttcaa
    17881 cagtttttga aataatctta cgaacttgat caaagctagg ttggtctgac aagccacgta
    17941 attcataaag tggttgaata ccgcgagcct taaagaaatc agctcccatc ccaccgatac
    18001 agatcatttc aaaaccttta ccgtctgggt ggtattcttc tttcaactcc ataacagctt
    18061 tcaaaataga ggaattataa cctccaacca aaccgcggtc tgaagtgata acgatatagc
    18121 ctgtcttctt cacagaacgg ctaatcaaca tcggattagt tgaagcacca gctccattac
    18181 catgaaggat atctgtcaaa agtttacgca ctttctgagc gtaaacttgg aagttgcgag
    18241 cagcttcttc agaacgacct agcttagcag ccgataccat ttgcatggca ttagtaattt
    18301 gactcgtatt ttttgttgag gcgatttttg ttttaatatc atttagagat actgccatct
    18361 gacacctcta ttcttattgg aagctagatt gattgagaaa ctctgtaatc gcagcatcca
    18421 agactgcttc ttctggcaag tcttttgtat cacgaatggt ttccaaaatc tctggatgtt
    18481 gagcatcaaa gaaggcatgg aactcttcct cgaaacgaac aatatcatct actggaacag
    18541 tatccaagaa accatgtgtc aaagcataaa gaatggttac ttgtttctca acaggtaatg
    18601 gtttgtgaac aggttgtttc aagacctcaa cggtacgacg tccacggttc aacttagcct
    18661 gtgttgctgc gtccaagtca gaaccaaact tagtaaaggc ttccaactca cggtatgaag
    18721 caaggtcgat acgaagtgta ccagcaacct tcttcatggc tttgatttgt gcagaaccac
    18781 ctacacgaga tacagatgaa cccgcatcga tggctggacg aatacctgca ttgaagaggc
    18841 catcgccaag gaagatttgt ccatcagtga tagaaatcac gttggttgcg atataggctg
    18901 agatatctcc tgcttgtgtc tcgataaatg gtagggctgt aattgatcca ccaccaagtt
    18961 catcagaaac tttagctgag cgctcaagca aacggctgtg gagatagaaa acatcccctg
    19021 ggaaggcttc acgacctgga ggacgacgaa gcaagagcga cagttcacga taagctaccg
    19081 cttgttttga tagatcatcg tatacaatca aaacatgctt accttgatac ataaattctt
    19141 ccgccatagc aaccccagca taaggagcta ggaagagcaa tggagatggt tgtgaagcag
    19201 aggctgtcac aacgattgtg tagtccaagg caccgtactg acgaagtgtt tctacttgcg
    19261 tacgaactgt tgattctttt tgtccaatcg cgacgtagat acagatcata tcttgatctt
    19321 tttggttcaa gattgtatca atcgcaatgg ttgttttccc tgtctgacgg tcaccgataa
    19381 tcaactcacg ttgaccacga ccaatcggta caagggcgtc aatagctttc aaaccagttt
    19441 gcaatggttc tgaaacagac ttacgttgca taacaccagg agctggtgct tctactggac
    19501 gagttttatc agtgtggatt tctccaagac cgtcaactgg acgaccaagc ggatccacaa
    19561 cacgaccaat cagactttca cctacaggga cttccatgat tttccctgta cggcggattg
    19621 tatcgccttc acggatatct gtaaagtcac ctaggatgat aataccaacg tctgttgact
    19681 ccaagttttg agccatacca taagagccgt tttcaaaatt caacaactct ccactcatga
    19741 cattttcaag gccgtgagca cgcgcgatac cgtccccgat ataggttaca acacctgttt
    19801 cagtcacatc aaaattgggt ttgaaatttt caatttgttg cttaattaaa gcgctgattt
    19861 cttgtgcgtt aattgccaaa agaacaccac tttctatttc aaattttctt taacaacttt
    19921 aagttgttgt ttaatactca catcaattgt cttgtgattg gcaaaaatga caaaaccacc
    19981 aatgagactt tcatcgattt gttcttttac actccttact ttcagagaca tttttttctc
    20041 aatcaaaggg agcaaacgag tcttctgttc atcagttaga ggatgagcag acgtaatcgt
    20101 cacttcaaat cgatttgttt ctttttcaag tcggttcaag caatctacaa gcacatcata
    20161 aaaaagattt gctctgtgat tgtaggccag aacctggata aagttttgta ataaaggtga
    20221 cacagaatct tggaaaaaag caattgtttt ttccttatcc gactcgtcta ctgccacttg
    20281 ttttaaaaaa gaaggcagac ctgttttttc aacaacttgc ttgatttgag tcaagtctga
    20341 aaagatacgg tcttcttctc ctttttcaag taccaattgg acaaaaggca tgctgtattt
    20401 ttcaattacc tttactgttt tcttgtccat taagcttctc ctagctgatc gatatactga
    20461 tcaatgagtg ctttatgggc atgactgtca aggttttgtg agatgatttt accagctaag
    20521 ctgatggtca aatctgcgac ctcaccctta acactctgta aagcttctac tttattttga
    20581 gcaatttctt gattggcttt ttcttttaag tgtcctgctt ctagtttagc atctgctaag
    20641 atattagcct tactttgctc agctgtttcc tttgcatttt caatgattgt cttagcttct
    20701 ttacggctac cagccaattc atcttcgcgt ttttgagcca atacttctgc tttttgacgg
    20761 gcttcttcag ctctgtcaat atctgaagca attttttcag ctctttcttc gaaaatgcct
    20821 gtaatattag accatgcaaa ttttttaatc aagactagca aaagaataaa agagccagtg
    20881 attaaaataa aattaccaat taattcacct actgttacgt gcatcagtta ctcctttcta
    20941 ttcctcttca atattaatct tattccctaa atacacagat gtcaaaagag taaaaacata
    21001 agcttggatg caggaaataa agacagaaaa tgcagtccaa gccaaattag ctccaaaggc
    21061 tactggatac caataaatag cttggtggga aagaagaagt aacaaactcg tcatgacctc
    21121 tcctgcaaag atattcccaa aaatccgcaa agccaaagat aagaagtttg taaattcttc
    21181 caagatattc atcggtatga caaaaacagg actcataaaa gattttatac tttttttaaa
    21241 tccacgacga cgaacgcttt ctatatgtgt caacaaaatg acaagaaaag ataaggttaa
    21301 gtcatactgt aaattagcgg ttggcgaact ccaccagtta gtcccatcga tcgtttgaag
    21361 ctttgtcatt aagccaaggt tattggcaat caccatgaaa aggaataaac aaaggaaaaa
    21421 gagtgagtaa tctttcatgt agcgcgaacc aatgttaggt tctgtaaatc caataacaaa
    21481 gtcatagaca tactcaagta cattttgctt tcctttgggt ttcaaggtca tattgcggct
    21541 tgcccaataa ataaagacaa aaataactcc cacaatcaac aaagtcatgg ctaacatagt
    21601 cagattgaag ataacaggac caatagagat gattggatta atactttctt ccatctaaaa
    21661 tcctcccttt tcttctcgtt ccatgtattt atttgataat aaatgagaag acaagagtta
    21721 caaagaaagt tccttcaata aaggcaacac ctaaaaacat caaactacga aactcagaaa
    21781 gcatatctgg ttggcgtgct actgatttaa acagtccatt catcaataaa ccttcaccga
    21841 cagatacgcc catacaggca atacataagc ctaaaaatgt taaattcata acaaattctc
    21901 ctttaaaata aaatttactt acttagtgta gtcctaaaat ttaaatttgt caacttttaa
    21961 ccgctattgg aagcgtttaa taaaaattat ttccattttg ctaaatttca agttcaagtt
    22021 agccatccag aagtcttctc tgggtgactt gtagtccaag tattttttag gatagttgtt
//
LOCUS       DAVLVZ010000029        21452 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000029 DAVLVZ010000000
VERSION     DAVLVZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.029"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(223..440)
                     /locus_tag="VYN77_001128"
                     /pseudo
     CDS             complement(223..440)
                     /locus_tag="VYN77_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(731..964)
                     /locus_tag="VYN77_001129"
     CDS             complement(731..964)
                     /locus_tag="VYN77_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEV1558598.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAVYSIHFNNGYCN"
     gene            complement(1178..2809)
                     /gene="rlmD"
                     /locus_tag="VYN77_001130"
     CDS             complement(1178..2809)
                     /gene="rlmD"
                     /locus_tag="VYN77_001130"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1558599.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKIFMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2883..3857)
                     /locus_tag="VYN77_001131"
     CDS             complement(2883..3857)
                     /locus_tag="VYN77_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1558600.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSELAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3931..4545)
                     /gene="pmp23"
                     /locus_tag="VYN77_001132"
     CDS             complement(3931..4545)
                     /gene="pmp23"
                     /locus_tag="VYN77_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1558601.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4545..5519)
                     /locus_tag="VYN77_001133"
     CDS             complement(4545..5519)
                     /locus_tag="VYN77_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1558602.1"
                     /translation="MDIYIKKAIIHQFSLDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(5527..6783)
                     /locus_tag="VYN77_001134"
     CDS             complement(5527..6783)
                     /locus_tag="VYN77_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1558603.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(6845..7276)
                     /locus_tag="VYN77_001135"
     CDS             complement(6845..7276)
                     /locus_tag="VYN77_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1558604.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7278..7877)
                     /locus_tag="VYN77_001136"
     CDS             complement(7278..7877)
                     /locus_tag="VYN77_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1558605.1"
                     /translation="MDRIRQELENGGAVILPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7864..8703)
                     /gene="prmC"
                     /locus_tag="VYN77_001137"
     CDS             complement(7864..8703)
                     /gene="prmC"
                     /locus_tag="VYN77_001137"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1558606.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALDLASENAKNQNLQ
                     IFFKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(8703..9782)
                     /gene="prfA"
                     /locus_tag="VYN77_001138"
     CDS             complement(8703..9782)
                     /gene="prfA"
                     /locus_tag="VYN77_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1558607.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLKELNK"
     gene            complement(9792..10271)
                     /locus_tag="VYN77_001139"
     CDS             complement(9792..10271)
                     /locus_tag="VYN77_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1558608.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIVISSGNYLLFCFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(10261..10860)
                     /locus_tag="VYN77_001140"
     CDS             complement(10261..10860)
                     /locus_tag="VYN77_001140"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1558609.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            10983..11165
                     /locus_tag="VYN77_001141"
     CDS             10983..11165
                     /locus_tag="VYN77_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1558610.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            11302..12675
                     /gene="mnmE"
                     /locus_tag="VYN77_001142"
     CDS             11302..12675
                     /gene="mnmE"
                     /locus_tag="VYN77_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1558611.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITKLF
                     SQFCLGK"
     gene            complement(12722..13657)
                     /gene="dapA"
                     /locus_tag="VYN77_001143"
     CDS             complement(12722..13657)
                     /gene="dapA"
                     /locus_tag="VYN77_001143"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1558612.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGFINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13713..14789)
                     /locus_tag="VYN77_001144"
     CDS             complement(13713..14789)
                     /locus_tag="VYN77_001144"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1558613.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(15131..15203)
                     /locus_tag="VYN77_001145"
     tRNA            complement(15131..15203)
                     /locus_tag="VYN77_001145"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15168..15170),aa:Thr,seq:ggt)
     gene            complement(15303..15812)
                     /locus_tag="VYN77_001146"
     CDS             complement(15303..15812)
                     /locus_tag="VYN77_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1558614.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLIDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15810..15906)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15935..16366
                     /locus_tag="VYN77_001147"
     CDS             15935..16366
                     /locus_tag="VYN77_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1558615.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            16448..16825
                     /gene="mscL"
                     /locus_tag="VYN77_001148"
     CDS             16448..16825
                     /gene="mscL"
                     /locus_tag="VYN77_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1558616.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16867..17961)
                     /gene="hemH"
                     /locus_tag="VYN77_001149"
     CDS             complement(16867..17961)
                     /gene="hemH"
                     /locus_tag="VYN77_001149"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1558617.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            18115..19338
                     /gene="pepT"
                     /locus_tag="VYN77_001150"
     CDS             18115..19338
                     /gene="pepT"
                     /locus_tag="VYN77_001150"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1558618.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(19374..20003)
                     /locus_tag="VYN77_001151"
     CDS             complement(19374..20003)
                     /locus_tag="VYN77_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558619.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            19968..20189
                     /locus_tag="VYN77_001152"
     CDS             19968..20189
                     /locus_tag="VYN77_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558620.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNIL"
     gene            complement(20460..20808)
                     /locus_tag="VYN77_001153"
                     /pseudo
     CDS             complement(20460..20808)
                     /locus_tag="VYN77_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145489.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(20912..21452)
                     /locus_tag="VYN77_001154"
                     /pseudo
     CDS             complement(20912..21452)
                     /locus_tag="VYN77_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6216 a         4462 c         3956 g         6818 t
ORIGIN      
        1 taatatctct ttcttctgat tttgtttcaa ggattattgc tacatcattc atatttttag
       61 gtaaatacca accatctggc ttatctgaat atcccttgaa gcctaattga ttaaaagtag
      121 ttatttgacc agtaccttga ttgatgtttt cttcaacttc attaaagcct atacttctct
      181 tgcataatcc ctaacttgat cttctgtcct taaagtcatt ttctattcct cgtcactaaa
      241 attatcttca aaactaataa tatcattagg agtgcaagat aaagccttac atattctttc
      301 taaattctta aatgtaattg gtttatcctt tcccatttgt gccataacat ttgttgtaag
      361 tcctgccata gctattacat ctgttttctt tagtcttttt ttgctaactg tatccataat
      421 ggtttatagt taagtgccat aatatccctc tttttttctt cattaaattt atttaattat
      481 agcataaata ttgatttcat tcaatcttac attgatttag tgatttgttt tagagaattt
      541 gttgtgttat tccgtcctta ttttgccata aattgtttta agtgggacct cttatcctta
      601 ccatttttac ctcctaattt tgagtataaa aaagacgaca gagtttttaa tttctatcgt
      661 ctgagattta catattaaat tttcatcaca aaataagact ctttaaagaa tctttaaatt
      721 cattggtatg ctaattacaa tatccattat taaaatggat actataaaca gcacaccaac
      781 tatttatttt ttgagtttta aactttggta gaagtcttga actccttata tttacataac
      841 tatttgcctc tataactatt atgacacgaa accaacaagc aagttcaagc acctccatat
      901 ctttattcaa atgaggacta agatattcac aaactttatc ataaacctct ttatcttttt
      961 tcataaaagg ggcatacaac ttagcaaatt tatcctaaaa ttttttgttg cctttatcaa
     1021 tcatttcaac actcctttaa atataaaaat gatcctatca atctttatat tgagcctatt
     1081 ttctaattga taccttgcaa gaaaggaata atagctgttc ctataccaag taggaatgta
     1141 ataaatgcag aaagtcatat actgtcattc ttttctatta aatcattttg aagtgtctca
     1201 aagcatccat gatggcttct tctttttcag gtgtacactg tggaataatt tgtttatcct
     1261 ttttagactt gttgtaatgt tctcgtaatt ctattccaca tttctttttt atctgtgcaa
     1321 tatataatgt cgaaactttt aattcaaatt tattccaaac atattctttg atttgagcat
     1381 atgttgcttt gctctccgca cttgtcaaat ccatctcatc cagctcaatt tcaacactta
     1441 tgtgcttatc gacatcaagt ttggacaaaa gtgctaccgt ctcgacgtga tgcgtttgag
     1501 gaaatagatc caccggctgg actttcttca attcatatcc caattcttgg tagagtttga
     1561 tatcacgcgc catggttgcg acattgcagg agatataggc gatgcgatcg gctcctgttt
     1621 gggcgcttgc tttgataaag ctttctgtca agcccttgcg tggaggatca accaagataa
     1681 cggttggttg aatcccatct ttaagccaat tcttcatagc attttcagct gtgtcacaga
     1741 catagtgggc gtttgaaata ttgttcagct gagcattttt tttactattc tcaaccgctt
     1801 ctggaatcag ttcaacaccg tagacttctt tgacatgctt ggcgactgat aaaccaatgg
     1861 ttccaatacc agaataagca tcaataacca cgtcatcttt ttttaactct gcaaagtcaa
     1921 tggctgtttg atagagtttc tccgccattt cagtattgac ttggtaaaag gctgggccag
     1981 cgatttggaa gtcatttccc aacatctggt ccgtaatata gtcttgacca taaagagtgc
     2041 gccactcctt accaaaaatc gcattggtat tctggtcgtt gatattttgc atgacagaca
     2101 caatctctgg gaactgcttg ataacttgtt caatcaattg gtcaacacga aaaacttttg
     2161 gacgagttgt caccaaaacg accatgattt gtcctgaata gtgaccacga cgcaccacaa
     2221 gattccgaat caatccagac tgttcctttt cgtcataagg ttttaaatca aaacgacgga
     2281 gcaggtctcg aagagctact acgacttggt caatgacagg atcctggata aagaaatctt
     2341 caaggggcat gaggttatgc gaattcttac ggaaaaatcc tgtttccaag acaccattca
     2401 ctcgacgaac gggcacctgc gccttattgc gatacttgac tggatgttcc ataccaagcg
     2461 tttcagcaac ttctacatct gcaattccag caatcttgta gagactgtcc ttgacttgct
     2521 tggttttaaa cttgagctgt tctggataag aaaggtgtcc taaatccgcg attcctgaac
     2581 gcaggtaagc caaatctaga tcttgattac ggtgtggtga ctggacaagg tatttttcaa
     2641 cttttccaaa gccaatcttt ttattgacct tgaggacacg catgaaaatt ttttcactcg
     2701 gtaaagcatt ctctacaaaa aagaccaaac catctacctt ggcaactcct gccccttcat
     2761 gggtcaaatc aacaatttca acttctacaa tatcattttt ctttaacatt cttttctctt
     2821 tctattggcc gacaaaaaag acggcaacgt tacggttacc atccattata ccatgaaatt
     2881 tcttaagaac caaaactctt gaagaagttg acaagggctt gccagaggga gcttaagaag
     2941 ttaccaccgt cctctagcgc cttatccgca tcaaagttaa ggttgatatt tttaaaactg
     3001 tcgccagctt gtgatacgat gctttgttta aggtcattta gggttttagt gaaatctgca
     3061 ttgctgagga tatcactctt tgagagattc aaggcaaaat tgatgatgat attgatctgg
     3121 tttcctgtta tgacctgatc aagtttgtaa ttttttaagg tatcttcaac aatcttgcgg
     3181 atatcttctt ctgtcagatt tcccttgctt tctttagctt tggcgagttc tgacttgata
     3241 tcagctaggg caacgtttaa tttattagca tcatagcctg atttgtcctt gttttcagca
     3301 ttgatatctg acaaagcctt tagctcttct tgagccaaat ctttattggc ttgcggcacc
     3361 ttggctccat tagcttctag cgaatagtag atcccagcca aagcactctc acctgtaact
     3421 ggaatgggtg ctgctacagt gattttggcg tgttccacac ccaaagttac ggctgcattt
     3481 cggtacatat cctgagttac cttagtgatg ttttcaggtg tttcaatctt gacctcaagt
     3541 ggcgatttgt cacctagctt ttgaatcttg gctgatgaat acaactgtaa gctagagtca
     3601 ttggccacat tcatgatttt agaataaaca tcaggtgtca tggtcttgag ttctttggta
     3661 tctgttgagg cattgtagcc cagtttttta agagtttgat ttttttggtc ttcagatagg
     3721 gaagaaccta ggacatattc aggttggaca taggtttcat cgataacttt ttgaacatct
     3781 gttgctgcat ggacgctatt catagctgtt actgcccaca agatcgcagc gctagtcaga
     3841 aagagtttct ttctcatagg gaatttcctc ctttacttct ttagagtaat atatctatct
     3901 taaagaaaac ttataacaaa aacacctggt ctagccagat gttgaaaaga gagtgaaaca
     3961 tttgatgatg taaaggttaa gtcgtacctg tctagaataa taatagtttc ctccatttac
     4021 atagagttca gcaccgtgaa aaatggaaat ggggtgaata taactataag tctttccagt
     4081 cgtattacca agcaaggggg caacagtctc acgagagtac tgtttggcta gagccaaggt
     4141 attttccttg ccattttggg cgataaaatc gatataggca ggtccaaaat tataggcttg
     4201 aacagctgtc cagatatcta cacccttctt ctgcgccaga tagagattgc ctgtcagagt
     4261 ttgaatgcct tgccgaatgc tagaggcatt atcattgatg gtgttggtgg aaccacttgc
     4321 agactcacta gactgcataa catcgccttc ttttcctttt gtttcagtat aaatcatagc
     4381 aagcacaagc tcttcgtttg ctggggtgtc tttttcactc aatatttctc gcaccatggg
     4441 ttgataggtc atgacttgtt tgacatcttg atgaacgcgg taagctttat agccagcaaa
     4501 aaggaagact gctagtacaa gcactcttcg aattcgttta aacatcattt actttggata
     4561 tcctcgatat ttttgattaa gatagagtag gttccatttt cgttttggat aaactcaaca
     4621 gactcggcgt cttgatagac gttattggga acgatgagct caattccatt tgataaggag
     4681 agtttttggt tttcaaattt ctttaattgg cgactggcat caatttcatc aaattgaaca
     4741 ggttctggta cggcttcttt gacttggtca ataaagctca aacgagccgt cagattgttg
     4801 tcaaaaaggt cattagccaa tttctcaggt gacaattcat tgctttcttc taggttgttg
     4861 aaaatagctg atttgacctt ggattgaaat tgaaaatcat ctgtgttaaa agattcagca
     4921 attctctggg ctgttttttc cagttccttg atagattttt taggagaaat cttaggagcg
     4981 acagcaagaa gattatctga aaaatagttc aaaaaagtcc cgttgtactt gattcgtttt
     5041 tcaatcaggt gatacttgcg actctgaaga ttgaccacca aggcctcatc agctcccgtt
     5101 ccaaatccag gcaggttatt ctgagtcagc ttgattggat tatcaacttc tcctccgagg
     5161 tgggtcaagg tctcccgcag ggcaattcgc aagaaagcga aatgttctac accttcttta
     5221 gaaaattgaa caaaaatcaa gtcattggtc ttgagatttt cagaaatgct aaactcctct
     5281 ttccagagat tagccagcgt tactgatgtc tccaacaaat cgtctgtaat atgattgaag
     5341 aagggatttt cttcttcgaa aatcccagtc ttggcttcat ctgaatacac atgttcaatt
     5401 tttttacgta ggtattcttc gatttttgga gtaatattga gaaacttatc tgctaagaac
     5461 agctcggtat catccagact gaactggtga ataatggctt tcttaatata aatatccata
     5521 aaagttttag tcctcgtata atgggaaggc atctgtcaat tctttgactg cacttctcac
     5581 ttcttctaat acagcctcat tttctgaatt cttaagggtt ttaatgatga gttcagccac
     5641 tttgcgactt tcttcttcac caaatccacg tgcagtaata gctgctgctc cgatacgaat
     5701 cccacttgtc ttgaatggcg acaaggtttc gtaagggatt gaatttttat ttagggtaat
     5761 attgacttca tccagcaagt tttgagcaac ttttccgttt tctacaacct tagtcacatc
     5821 aacaaggaag agatggtttt cagttccacc tgaaataata cggaaatcag ggtcttgcaa
     5881 gaagacatct gccatagcct tgctgttctt aattacattg gcagcatatt ccttgaaggc
     5941 tggatccaaa acttctttga aggaaactgc cttagccgcc acaacatgct ctaaaggacc
     6001 gccctgaata cctgggaaaa tagctgaatt gattttttta gcaagttctt cgtcattggt
     6061 caaaatcaaa ccaccacgag gtccacgaag ggttttgtgg gtcgttgttg ttgtgatatg
     6121 agcgtatgga actgggcttg gatgaaggcc agccgcaacc aaaccagcga tatgggccat
     6181 gtccaccatg agcttcgcac cgacagcatc tgcgatttca cggaattttg aaaaatcgat
     6241 aatttgagaa tatgctgaag caccagctac aatcagtttt ggttttactt cttgggcttg
     6301 tttcaagata gcatcaaagt ctaagagttc cgtttcagga tcaacactat aagaaacaaa
     6361 gttgtaggtt tgaccagaga agctaacagg agccccatga gtcaaatgac cacctgatgc
     6421 caaatccatt cccataaccg tatcacctgg ctcaatcaag gacatgtaag ccgcacagtt
     6481 agcttggctt cctgaatgtg gttggacatt ggcaaattta gcaccgaaaa tttcttttgc
     6541 gcgttcaata gcaagagtct ctacaacgtc tactacatca gttccaccat aataacggcg
     6601 tcctgggtaa ccctcggcat atttatttgt caagatagac ccttgagctg ccataacagc
     6661 cttggaaact acgttttccg aagcaattaa ctcgatatta ttttgttggc gttcttcttc
     6721 tttggcaata gcattccaga gatcagcatc atatgcttta aaatcatctt tgtcaaaaat
     6781 cataggtctt ctcctttatt gtgtgactag tccattagtt tgattttaca ataagaaaat
     6841 caaactaaaa gatgcgaata aaccgtttct gcattttatc acaagtatag ccaacttttt
     6901 cataaaatgc atgagcaccc agacgatgat tggcagaatt taagcggata aacccataac
     6961 cacatctttt ggcttcttgt tccaaccctt gtagtaaact tttaccgata ccttgacctt
     7021 gcgcttgagg tgaaactgct aaagctaaga tattaaatcc tgctttggaa tagagtgatt
     7081 cgtaaacttc agcgtggaca tatccaagta agacatgatt agctgcatcc tcatagccaa
     7141 gtaggaaatg atgggaatcc tgagacagtc tagctagttg gctagccgtt tcctctggac
     7201 taaaagtata acccaaagcc tcttggttga tgtcacatat cgctttcaca tctgtttctt
     7261 gcaaatctct tagcatttca agcctcctca aaagaaatct ctggcaaccg agcaagaata
     7321 tcttctcgtt taattgcgcc ttggcgtaag attttcacct tgtctccaga caaatccaca
     7381 atagttgaat cctgtccagt tagaaaagca tcgtcttcca gacccagaac ctcttggtca
     7441 aaatccttca gaatttgttc aaaggttaca ccacttgcct gacctgagat attggcagac
     7501 ggcccaatca agggacctgt ctctcgaatt aaatccagtg tgatagggtg actgggcatc
     7561 cgaaatccaa tagttgcaag gtcagaattt acccaatagg gaactcggtc attggcttcg
     7621 agaataatgg tcaagggacc tggcaaaaag gtctctacaa gtttttgtag ataagctggc
     7681 tgattcttag aaaagtgcaa gatatcctct aaagaggcaa cattgagatt gagcgccttg
     7741 tctctaggac gacgtttgag ctggtaaaca tggtcaactg ctttttcgtc taaggccttg
     7801 gcaaaaagac cataaaccgt ctctgtaggc agaatgacag ctccaccatt ttccaactct
     7861 tgtctaatcc tgtccatcat caaccacgac catcctatct tgaccaaatt ggtccttgag
     7921 tgttcgaact tttttttcag gaagatgttt tctaaaaagt tcgggaacac tttgaccttg
     7981 cttgtatcca atttcaaggt aaatcttacc accatctttg agatagtctt ttgcatcttc
     8041 cgcaattcta cggtaaatag ctaggccatc ctcgtctgca aagagagcta gatgaggctc
     8101 cgaatacaag acattcaagc ctacctctga ctcatcttca cgagagatat agggtggatt
     8161 ggaaacaatt atatcatatt tttcagaaat ttctgtaaaa cagtcagatt ttttaaaaaa
     8221 tatttgaaga ttttgatttt tagcattttc gctagctaaa tctaaagcct cttgagaaac
     8281 atctgctgct gtcactgacc aatctggtct attttttgct agagcgagag caatagctcc
     8341 actacctgtt ccaatatcca gaactgaaag attcgtctca agattttcag ttagaataag
     8401 ctccactaac tcctctgttt ctggacgagg aatcaaaacc cgttcatcca cctttaaatg
     8461 cattccataa aaatctgcct gtccaatgat gtactgagct ggcttgtgag ctgctagttg
     8521 ctggtaaata tcttctacaa attgtttttc ttcctctgtt gtcacctcct gctggagggc
     8581 aaaaataaag tctgtaaaag atagattttt cagactacga tagacaaaag agaggctttc
     8641 cgcttcctct ccttgtctta tcaactcttc ttcaaaattt gaaaataatt gagctaattt
     8701 cattatttgt ttaattcttt tagtttttgt gtttggtcat aaagcaccaa ggcatccaca
     8761 acttcgtcca atttaccaga caaaatcgta tctagttttt ggagggtcaa gccgatacgg
     8821 tggtctgtga cacggttttg tgggaagtta taagttcgga tccgttctga acggtcacca
     8881 gtaccgattg tcgacttacg ctcagcgtct tgttcatcct gagcaatctg agcaaagtgg
     8941 tcagcgacgc gtgcacggat aatcttcata gccttctcgc ggttcttctg ctgggtacgt
     9001 tcttcctgca tctcaacctt gatattggtt ggcaagtgaa cgatacgaac ggcagtcgca
     9061 accttattga cgttctgtcc accagcacca gaggcgtgat agatgtcgac acgaaggtct
     9121 tttggatcaa tgtcgtattc aacctcttca acttctggca taacaagaac tgtcgctgtc
     9181 gaagtatgaa cacggccttg gctttctgtc acaggaacac gttgcacacg gtgggcacct
     9241 gattcatact taagcttaga gtatacagac tgacctgaaa ccatagcaac cacttcttta
     9301 aaaccaccga caccattcat agaggcttcc atgacttcaa agcgccaacc ttgggcttcc
     9361 gcatactttt ggtacatagt tagcaaatct ccagcgaaaa gtgccgcttc gtctccacca
     9421 gctgctccac ggatttcaag gatgatattc ttgtcatcgt ttggatcctt tggaaggagc
     9481 aaaattttca gtttttcttc atattcttct ttttcagcct tggcatcttt gagttcttgc
     9541 ttggccattt cttccaagtc cgcatctccg cctgattcct taatcatctc ttcggcatcg
     9601 acgatatttt gaaggacttg tttatactca cggtaggcta ttacggtgtc acgattggaa
     9661 gcttcttctt ttgaaagctc cataaaacgc ttggtgtctg aaacgacatc agggtcactc
     9721 agcaattctc ctagttcttc ataacggtct tctacagctt gtagttgatc atagatgttc
     9781 attttttctc cttatttctc aattgttaaa tcatagattg ctactacttc attctcggat
     9841 atttccccag tttctttaaa tccataactg agataacaaa atcttgcctg ttcattttct
     9901 ggttcataag acaaccaaag tttattgctt aaacctgctg gcgctgttcg aacatagtct
     9961 agtactttat ccataattgg tttaaaatat ccttgatttt gaaaattctt atcaatcata
    10021 aaacaaaata gtaaataatt tccactacta attacgatct ttttatcata agctatcatc
    10081 acaaaaccta taattacatc attatcataa actgccaatg gagctacaaa atctccattt
    10141 ttagtgtaga cgtatgcttc agctaaacta attgcgttgg ttgcaatgaa ttgtttttga
    10201 tattccttga catccaaatt taaaacatca aaataatttt ccattgtaac atctcttagt
    10261 tcaattgtca tagttttgct ccttgttaga ggttatcatt ggcgcaaaat aatgtttacg
    10321 gcaaaccgag atataggttt cattaccacc aatttggatc tgttctccat cgtaaacggg
    10381 cagtccatcc tgtgttcgca acaccatggt cgcctttttc ttgcaatact gacagatggt
    10441 cttgatttcg tcaatcttgt ctgctaaaag caagagatat ttggaacctt cgaacaattc
    10501 attgcgaaag tcatttttca agccaaaagc catgacgggt atgtctaact cgtccacaac
    10561 acgagctagg tcataaacat ggtggcgttt gagaaactgg gcttcatcga ccaacacaca
    10621 gtaaggtttt tctggtaggc ctcggatata gccaaagata tccgttgttt cctcaatcgc
    10681 aagggcaggg cgtttcatgc caattcgact cgacacatag ccaacaccgt cacgcgtatc
    10741 cagagctgaa gtcataatca caacaccttt tccttgctcc tcatagttat aggccacctt
    10801 gagaatctcg attgtctttc cagagttcat ggttccataa cgataataca gctgtgccat
    10861 gtttcttgct tcacgtccat ttctaaattt ttgctacatt ctagtatatc ataattttct
    10921 taagctttaa acggcaaaat gtggtaaaat ggaagaaatc aaaactagtg gaggaagcta
    10981 ttatgccatt tgtacgcatc gatttatttg aaggacgcac gctcgagcaa aagaaagctc
    11041 ttgctaagga agtaacggaa gcagttgtcc gcaacactgg agcccctcaa tctgctgtcc
    11101 atgtcatcat caacgacatg ccagaaggaa cttacttccc acaaggggaa atgcgtacta
    11161 aataagctag cttaagcaga attgcttagg ctttttcaat ctccaagtag cattcattga
    11221 agaaatatcc taaatttgtt acaatttgaa aagaaacttg gagaatttcc aagaaaatag
    11281 ctattaatta aaggaaacat tatgattaca cgtgaatttg ataccatcgc tgctatctct
    11341 actccactag gtgaaggggc tattggtatt gtccgcctga gcggaacaga cagttttgct
    11401 attgcgcaaa agatttttaa aggaaaagac ttgaacaagg ttgccagcca cactctcaac
    11461 tacggtcaca ttattgatcc tctgactggt aaagtcatgg acgaggttat ggttggggct
    11521 atgaagtctc caaagacctt cactcgtgag gatattatcg agattaacac ccacggtggt
    11581 attgcggtga ccaatgagat tctccagcta gctatccgtg aaggagctcg gttggcagaa
    11641 cctggtgaat ttaccaaacg tgccttttta aacggtcgcg tagatttgac acaggccgag
    11701 gcggtgatgg atatcatccg cgccaagaca gacaaggcca tgaacatcgc ggtcaaacaa
    11761 cttgatggtt ccctttctga tcttatcaat aatatccgtc aagaaatcct taacacactt
    11821 gcccaagttg aggtcaatat cgactatcct gagtatgacg atgttgagga agccactact
    11881 gctgttgtcc gagagaagac aatggagttt gagcaattac taaccaagct ccttaggaca
    11941 gcacgtcgtg gtaaaatcct tcgtgaagga atttcaacgg ctatcattgg acgtcccaac
    12001 gttgggaaat caagccttct caacaacctc ttgcgtgagg acaaggctat cgtaaccgat
    12061 atcgctggga caacacgaga tgtcatcgaa gagtacgtca acatcaatgg tgtacctctc
    12121 aaattgattg atacagctgg tattcgtgaa acggatgata tagtggaaca aatcggtgtt
    12181 gagcgttcga aaaaagccct caaggaagcc aacttggttc tactagtgct aaatgccagt
    12241 gaaccactga ctgcgcaaga cagacaactt cttgaaatta gccaagatac caatcgcatt
    12301 attctactta ataaaaccga cctgccagaa acgattgaaa cttcgaaact acctgaagac
    12361 gttatccgta tttcagtcct taaaaaccaa aacatcgaca agattgaaga gcgaatcaac
    12421 aacctcttct ttgaaaatgc tggcttgatt gagcaagatg ctacttactt gtcaaacgcc
    12481 cgtcacattt ccctgattga aaaagcagtt gaaagcctac aagccgttaa tcaaggtctt
    12541 gagctgggga tgccagttga tttgcttcaa gttgacttga ctcgtacttg ggaaatcctc
    12601 ggagaaatca ctggggatgc tgctccagat gaactcatca ccaaactctt tagccaattc
    12661 tgtttaggaa aataagaaaa atccatgatc cttcattcgg tcatggattt tattgtcttt
    12721 attagtaatc tggtcttaag acccctgtta cagttgcctt agttgcttcg tagtcgccat
    12781 ctacgacaac cttgataatg cgtttggcat cttcttctgg tgctggaaca agaggtagac
    12841 gagtgggtcc agcttcaaat cccatatagt taagaattgc cttaactgga gcaggacttg
    12901 gataagagaa gagagcatta accttaggaa tgaatttacg ctgaattgct gcggctttct
    12961 tcatatcgct ttctgcaatg gcagtaaaca tctcgtgcat ttcatcccca tttgtatgag
    13021 aggcaacaga aataacccca tccgccccaa ggttcatggc atggaaagca tctccatcct
    13081 cacctgtata aatcaagaac tcttcaggct tgtgctcaat caagtaagcc atattagcca
    13141 agctagtaca ttctttgaca ccgataatat ttggatggtc agccaagcga agcatggttt
    13201 ctggagtcaa ttcgacaact acacgccctg gaatgttata gataataatt ggtaggtcag
    13261 aagcatctgc aatagtctta aagtgctgat acatcccttc ttgagaaggt ttgttgtagt
    13321 aaggaacaat agcaagccca gctgcgaaac caccaaattc cgctacttct ttgacaaact
    13381 caatagagtc acgcgtatca ttagtaccta cacccgcaat caaaggaacg cgtccattga
    13441 caaccttttg tacagccgca aacaactcca actcctcatc gtgggtcaaa gttggactct
    13501 cagcagtcgt tcctgcgaga agaattccat ccgtatgatg ggccaataaa tgctcaatca
    13561 aggctggaat agcatcaaag ttaataaaac catcctcatg aaagggggta ataaaggctg
    13621 taatgatttt acattctttt aaatcttgat aagacatgaa aacacctctc tatttcaaag
    13681 aaggagttca tctgaactcc taaacgatat gactatttta attcaaattt caattcggct
    13741 gttggacgaa ccaatccacg ttcatgaaga gtttcagcaa tctgaactga gttccaagca
    13801 gcacctttga gaaggttatc tgaaacaacc cacatgtgaa ttcctttttc tgcatccaag
    13861 tctttacgga tacgaccaac aaaggtatca cgcgaaccaa ctgcattgat agcttgagga
    13921 tagatttgat gagctacatc atcttcaaga acagcacctg ggaaggctgc gatagctgct
    13981 tttacttctt cgattggagc cacttctttt gtttcgatat aaacagactc agagtgagct
    14041 gacaagactg gaatacgcac acatgttgca gatactgcaa tgctatcatc ttccataatt
    14101 ttcttagttt ccttggtcat cttcatctct tcgtacgtgt aatcattatc agtgaaaaca
    14161 tcaatttgtg gaagagcgtt aaaggcgata ggatagtgtt tcttgtcacc acctgaaggc
    14221 aaaatttccg catgcaaatc acatggtttc acaccatcat tcaagacttc acgaagttca
    14281 cgttgtgtct caagaattgc tcccatacca gcacctgaaa cggcttgata agttgaaaca
    14341 atgatacggt ccaagcccca tttttggcga accggctcaa gagccaccat catttggatt
    14401 gttgaacaat tagggcaggc aatgattccg ttgtgggcat caagtgcatg agcattgacc
    14461 tctggaacaa ccaaaggaac atctggattt tgacggaaat aagatgtatt atccactact
    14521 accacgccag cttttactgc gtatggtgca tacttagctg atgtagaact acctgctgaa
    14581 aagagagcaa tatcaactcc ttcaaaagct gtttcagtcg tttcttcaat tgtaatatct
    14641 tgatctttaa atttcaatga cttacctgct gaacgtgcag aagcaaggta acggatttta
    14701 tcgattggaa gtgttgattc ttccaacatt tttatcatct gagcaccgac agcacctgtc
    14761 gcgccgacta cagcaactgt atatcccata aataacctct ttcggaattt tctaaaaatt
    14821 tctataatag aagtattata ctactttttc catgaattgc aaagcttttt catcattttt
    14881 tggaaaataa aaatcccagt cgctagacta gggactaaga ggcatcttca tgtgatgagc
    14941 aggttcacac aactcatcaa ggtccgctcc tgcgttatga cctccttatg ctcaatagta
    15001 gtccgaagac tacctatcga ctcatcgcat attctatatt actagagata cttgattttg
    15061 tcaataagaa attttttcga tcgagtataa caaaaaactc tcgtattcaa gagtttccct
    15121 atacatatca tgctaattgc cgggatcgaa ccggcgacct catccttacc atggatgcgc
    15181 tctgccaact gagctaaatc agcttactta agaagtatac tatattcaga aaaacttgtc
    15241 aagtttcctt agaaattttc cataggaaaa agccaggtaa gaactaccta gcttatcttc
    15301 ttctatttac ttaaatcaat tcgacgacca attttaccaa taataatcgc tccaataatc
    15361 aatgaagcaa attcaccgat tcctgttgta aaccaagtaa ggaagaatgg taattctgct
    15421 acaatattta actctgcagc gatagtaaac atggaaattg aaaagaggat tgaaaagaag
    15481 aaatgatctt ttcgaatcaa tccgttaaag aggtaatcct tgctatattt tgcaaaaagc
    15541 caaacaccta aactgaggaa tactaaggtt gaaccaccac caacaaagac atctatcagt
    15601 ccgaagctaa agaaattagc aatcatacaa ccgatggtaa ctccgatgat gtacttagga
    15661 ttgtaaaaag ccataaagtt catcatttct gaaatacgaa actgataagc accatagctg
    15721 atggcattta ggggcggtgt gacagtcaaa acgacgtaga tagcagcgac gattgcaata
    15781 tctgcaacat cacgaatagt taattttttc atcttttctc ctttggcggt tatccgcgtg
    15841 taatatgctt ggtgaaagaa gctaagcacc aagggttgat tcattcaacc ttactagtat
    15901 agcacaatag cctgctttat gctatactta aagcatgaaa aaacctattc aaaaattttt
    15961 taacaatgaa atcttagcct atctcttttt tggccttgcg acaactctag tttcgattct
    16021 ctcacgacta gtcatttatc aactaagtca tcaggaactc cttgctacgg ccttggcaaa
    16081 tattatcggt atcttatttg cctttatcac aaatgatacg attgtattta agcaaaaaaa
    16141 gaaaaataga cttatccgtt tagtgaaatt ttctcttgcc cgcctttcta cttttctgtt
    16201 agacttgttt ttaacttttc tctttgtaac ccaatttcct cacatcatag ggcaattcgt
    16261 aactgaaaac attgataaga taaatgctat tgaaacaata attgcacaaa tattcattat
    16321 tatcatcaat tatatcttga gcaaggtcta tatttttaga aaataatgct ccatttatga
    16381 gccttttaat tgccttctat aatgatttag gatacaatga aacacgaaac attgaaagga
    16441 aaacagtatg ttaaaaaatc taaaatcgtt cttgcttcga ggaaatgtta ttgaccttgc
    16501 tgtcggtgtt gtcattgcct ctgcttttgg tgctatcgtt acttcacttg taaacgacat
    16561 tatcactcct cttattttaa atcccgcttt gaaagctgct aaagtcgaac gtatcgctca
    16621 actttcttgg catggagtcg gctatggtaa ctttttaagt gctattatca attttatctt
    16681 tgtgggtacc gccctcttct ttattatcaa gggcattgaa aaagcacaga aactgactgg
    16741 cataaagaaa gaaaaaactg ccgaaaaaaa accaaccgaa ttggaagtcc ttcaagaaat
    16801 aaaagctctc cttgagaaaa aataatcgat taaaaggatc tagcgcaaag ctaaatcctt
    16861 ttttctttac tctttgggta acttgcctgc tttttcaagc atttttttga taagataagg
    16921 catcttaaca ttctcccgcc cttttttctc aatcagtttt ttcacaaatt ctggcatttg
    16981 taaatcttca gattcagcta aaatgttttt cgtctcgtct gaaggaacta actcatcaaa
    17041 ggtctcttct tctgggagaa attccttaaa ctcttgattt tcattgactc taactgtatt
    17101 gaccaaggca tcaatcaaac gagaatctgt atttggcatt ggtggacgat ggtagtttac
    17161 cccaaattcc tggcataagt cataacattc tacatcattg tcaaacaaga cctcaatatg
    17221 ctcactgatg aaactaatag gtacaaaaat ataatggtct ggatgttctg tctgttctct
    17281 gagatactcc aaaacatctg gtttaatcca tggaatacca atatcactct cactctgcca
    17341 agtgttggta tattgttctg aactcaagcc cagtttttca gcgactagct tgctattttc
    17401 gaaaatttgg tctatataag gatcaccaaa gtccaaggca aaaatgggca cactatgggc
    17461 tgaaaagatg actttaaagc tgtcttgctt tacttcttct tttaaaatct tagcaatttc
    17521 atctgcccaa tagtttaaga gcgcttcttc ctgataccag tccttaatga ccaaaaattg
    17581 aatttgtttg ctttctaaaa atttttcata gcccatgaca gagtagaaag aataatgggg
    17641 ctccaaaatc aagcaaatac actgttcaat tccgtctgct tccatttgac cgatcacatc
    17701 tggaataaag ggactggaaa acttattagc aaagtagaca ctgtattcat tccccagcct
    17761 agcttctacc aaggcaacct cttgacgggt aattttttgc agaggcgtgc ctccaattcg
    17821 tacataatta tcataaagag tctgaatctc gtggtcttgg ggtctcaccc cacgacgaat
    17881 atttgtgaaa aaatcagcta caccttcaaa ggtaatctct tctggtgaac cgaaggtcat
    17941 cattaaaatt gcttttttca tgactgcttc cttgtgatgt ttttatcaag tttattttat
    18001 cattaattca tcgataagta aacgctagca tagggacatt ctcaatcttc tgtcttttgt
    18061 ttttctcttc tttctatgat acaatggaaa aaataaattc aaaaggagtt ttttatgact
    18121 tatccaaatc tcttggaccg cttcttaacc tacgttaagg tcaacacgcg ctctgatgaa
    18181 cactctacta ctactccaag tacacagagt caggttgact tcgcaacaaa tgtcctaatt
    18241 cctgaaatga aacgtgttgg actgcaaaat gtttactatc taccgaatgg ttttgctatt
    18301 ggaaccttgc cagccaacga tccgtcttta acacgtaaga ttggttttat atcgcacatg
    18361 gatactgctg attttaatgc tgaaggagtc aatccacagg taattgaaaa ctacgatggt
    18421 ggtgtgattg aactagggaa ttctggtttc aaactcgacc cagctgactt caagagtctt
    18481 gaaaaatatc caggacaaac gctcatcaca acagatggaa caagcttgct aggtgctgat
    18541 gacaaatcag gaattgctga aattatgaca gccattgaat atctaactgc tcatcctgaa
    18601 attaagcact gtgagattcg tgttggtttt ggtccagatg aagaaatcgg tgttggtgcc
    18661 aataaatttg atgcagaaga ttttgatgtg gattttgcct acactgttga tggtggtcca
    18721 ctaggtgaac ttcagtacga gactttctca gccgctggtg ctgaattgca tttccaaggc
    18781 cgtaatgtcc accctggtac tgccaaaggg cagatggtca atgcccttca gctagcaatt
    18841 gattttcata atcaacttcc agaaaatgac cgacctgagt taactgaagg ttaccaaggt
    18901 ttttaccatc taatggatgt gacaggtagt gttgaggagg tgcgtgcaag ctacatcatt
    18961 cgtgattttg aaaaagatgc ctttgaagcg cgtaaagcat ccatgcaatc tatcgctgat
    19021 aagatgaatg aagaacttgg gagcgaccgt gtcactctca acttgacaga ccagtactac
    19081 aatatgaaag aagtcattga aaaagatatg actccaatta ccattgctaa agccgttatg
    19141 gaagatctag gtatcacgcc tattatcgaa ccaatccggg gtggaacaga cggctctaag
    19201 atttccttta tgggaatccc aactccgaat atctttgcag gtggcgaaaa tatgcacgga
    19261 cgttttgaat acgttagcct tcagactatg gaacgtgcag ttgataccat cattggcatc
    19321 gtagcttata aaggctaaaa agacgaggta gctcagctac ttcgcctttc tttttattct
    19381 actggttttt cttgatttcc agtagttgta gaagattctg ttgtttcatt ttctgaagtt
    19441 gattcagcag gtttagaatc tcttgtattg cttggtttgt tttcgtcgct agcagtttca
    19501 atgttagatt ctgcagttgc gtttggttgg ttctcagcac tggtgttatc actatttgct
    19561 tcagcatttc ttgctggact tgtttcttca cttgcgctag cttttgactg gatttgatga
    19621 ttcaaaacta gaatagcttt tgtcgattca agtaaagctg ttttgtcttt actcttagca
    19681 gaaagttgat ctaataatgc atccacctta tcaaagtccg catcagatcc attattactt
    19741 tctaaataag agtgaagcga catgagaata tcgtagagtt tttgatagag tacaagtgtc
    19801 tgagaatctt gctcagcatt ttccttttct tgttgaaggg cgctagcgat acgagtcaaa
    19861 acatctttta cctgactgtt tacttcatcc aagtctgcat cagccttgtt tgtggcagct
    19921 tttagatttt ctacttcttc tgccaaggat tgtctgattc cttcttcatg gatttgttcc
    19981 aagagttgat ttgccttgct caaaagactt tctacttctt ccttgctatc tgtcgcagat
    20041 tattggttgc tatctaccat gtactcctaa aacaggagag ttataatcca agattacaag
    20101 gccttacaga aataagaaat ccagataaga caatgttcgt ccaagacgct attcgcttcg
    20161 cacagcagca cggattcaat atactttaat tttaaagttt aggtgtcaag acctcttttt
    20221 agtgtgccca aaatttagag aagtaatcaa tcaactaact tttatttttt tcaaactttc
    20281 agtaaactga cctaaagcta actcaatctg tctttgtaga tgcttctgct atcagctaga
    20341 agttgatcta cttttgccaa gactgccttc tcatcaaaag ttccaggttg atagttggat
    20401 tgcagggatg gaatcttgtt tttcaaagcc gcttcatatc ccttagtttg aaccttgatg
    20461 tagtgattgt ggtcgccatg aggaatcaca aaaccttctg aatcttcact tataattcga
    20521 ttggcatcaa aaaccatgac catcttcttc ctcatggtgg acatgtagtg acggattact
    20581 taatacagaa ctagaagaac ttcctacctc ttccgtgtta gagtgtgatg ggggattgtt
    20641 aagagatgac ttaggaatat agtgatagtg ttccccatgt cttactatat aagcatcacc
    20701 tatatctctg acaatatcat tagggttaaa gacatatgtg actgctaatt cacctgccga
    20761 caagtcactc tcaggaatga aatgatagtg accaccatgt ggtactatag taggttgaaa
    20821 tagaatatga gcaaattgat aaggggattt taaagtaatt tctaacaatg atttagaaac
    20881 tatgatgtgc tattctaaat tcaactcact atatataacc atcatcggta gtataacgtc
    20941 cctgtaattt tgctacagat acttctgcac tagctccttt atcgtcttta ccatgttctt
    21001 gtttttggcg attgatttca tcttttgttc gtacattttc tgcatgagct tgatctttaa
    21061 ggtaaacata atactttcca tctaccttaa taatatatcc tcccttaacc taactgacga
    21121 tatcttgatc tttcggctga tagttggggg ctttcattag tagctcttca ctaaagagcg
    21181 catcaaaagg aactttacca ttatagtagt gataatgatc gccatgagaa gttacataac
    21241 cttgatctgt aatcttaata acaatttgtt ttgcttgaat tccttctttt tgactaacct
    21301 agtctggagt caaattttca gtcttcttag tgtctttatt actgtttaca tatgaaacac
    21361 gatttttatc tgtattggcc tgttagctat gttggttcag agcataaaca cacagactta
    21421 aggaaaggat aacaacagat ccagctgcta ta
//
LOCUS       DAVLVZ010000030        20959 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000030 DAVLVZ010000000
VERSION     DAVLVZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.030"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(274..838)
                     /locus_tag="VYN77_001155"
                     /pseudo
     CDS             complement(274..838)
                     /locus_tag="VYN77_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(914..1126)
                     /locus_tag="VYN77_001156"
     CDS             complement(914..1126)
                     /locus_tag="VYN77_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1558621.1"
                     /translation="MIQPLLHADETSYRVLENDSHLTYYWTFLSGKAENQAITLYHHD
                     QRRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(1135..1440)
                     /locus_tag="VYN77_001157"
     CDS             complement(1135..1440)
                     /locus_tag="VYN77_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEV1558622.1"
                     /translation="MFPVETEEITYKRKKSKGKRQAILAQFDSEEVHHQVEESICPDC
                     QDDLKEIGASLQRQELVFIPAQLKRVDHIQHAYKCQTCSKNNPSDKIVKAPIPKAPL"
     gene            complement(1427..1621)
                     /locus_tag="VYN77_001158"
     CDS             complement(1427..1621)
                     /locus_tag="VYN77_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1558623.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     EQLNLFEEESPSEKDGDVPS"
     gene            complement(1791..2141)
                     /gene="tnpB"
                     /locus_tag="VYN77_001159"
     CDS             complement(1791..2141)
                     /gene="tnpB"
                     /locus_tag="VYN77_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1558624.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESRDFY"
     gene            complement(2122..2307)
                     /locus_tag="VYN77_001160"
     CDS             complement(2122..2307)
                     /locus_tag="VYN77_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558625.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2611..4173)
                     /gene="guaA"
                     /locus_tag="VYN77_001161"
     CDS             complement(2611..4173)
                     /gene="guaA"
                     /locus_tag="VYN77_001161"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1558626.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4315..5013
                     /locus_tag="VYN77_001162"
     CDS             4315..5013
                     /locus_tag="VYN77_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1558627.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(5056..5952)
                     /locus_tag="VYN77_001163"
     CDS             complement(5056..5952)
                     /locus_tag="VYN77_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1558628.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGTLMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLIIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYTIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5961..6896)
                     /locus_tag="VYN77_001164"
     CDS             complement(5961..6896)
                     /locus_tag="VYN77_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1558629.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEY
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6893..7618)
                     /locus_tag="VYN77_001165"
     CDS             complement(6893..7618)
                     /locus_tag="VYN77_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV1558630.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7702..8337)
                     /locus_tag="VYN77_001166"
     CDS             complement(7702..8337)
                     /locus_tag="VYN77_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1558631.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(8339..9166)
                     /locus_tag="VYN77_001167"
     CDS             complement(8339..9166)
                     /locus_tag="VYN77_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1558632.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKVRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(9188..9328)
                     /locus_tag="VYN77_001168"
     CDS             complement(9188..9328)
                     /locus_tag="VYN77_001168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558633.1"
                     /translation="MYHYQTYILEDFIEDGSHCDQLDEATKERIDTAYKEMNEHVGLK
                     WY"
     gene            complement(9524..10240)
                     /locus_tag="VYN77_001169"
     CDS             complement(9524..10240)
                     /locus_tag="VYN77_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV1558634.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYIFQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGNKKLIIY"
     gene            10763..11374
                     /locus_tag="VYN77_001170"
     CDS             10763..11374
                     /locus_tag="VYN77_001170"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1558635.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11419..12147
                     /gene="rlmB"
                     /locus_tag="VYN77_001171"
     CDS             11419..12147
                     /gene="rlmB"
                     /locus_tag="VYN77_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1558636.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKNLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENSLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(12186..13097)
                     /gene="trxB"
                     /locus_tag="VYN77_001172"
     CDS             complement(12186..13097)
                     /gene="trxB"
                     /locus_tag="VYN77_001172"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1558637.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVRKIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(13163..13387)
                     /locus_tag="VYN77_001173"
     CDS             complement(13163..13387)
                     /locus_tag="VYN77_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1558638.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13465..14208)
                     /locus_tag="VYN77_001174"
     CDS             complement(13465..14208)
                     /locus_tag="VYN77_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1558639.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(14208..15008)
                     /locus_tag="VYN77_001175"
     CDS             complement(14208..15008)
                     /locus_tag="VYN77_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1558640.1"
                     /translation="MNVTTILASDWYQNLMQLIPNGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(15166..15522)
                     /locus_tag="VYN77_001176"
     CDS             complement(15166..15522)
                     /locus_tag="VYN77_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1558641.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15516..16046)
                     /locus_tag="VYN77_001177"
     CDS             complement(15516..16046)
                     /locus_tag="VYN77_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1558642.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRSNRRRSTC"
     gene            complement(16046..16540)
                     /locus_tag="VYN77_001178"
     CDS             complement(16046..16540)
                     /locus_tag="VYN77_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1558643.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16675..17121
                     /locus_tag="VYN77_001179"
     CDS             16675..17121
                     /locus_tag="VYN77_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1558644.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            17118..17765
                     /locus_tag="VYN77_001180"
     CDS             17118..17765
                     /locus_tag="VYN77_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1558645.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILTASVLQYIAIVYYM"
     gene            complement(17786..18367)
                     /gene="pdxT"
                     /locus_tag="VYN77_001181"
     CDS             complement(17786..18367)
                     /gene="pdxT"
                     /locus_tag="VYN77_001181"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1558646.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18368..19243)
                     /gene="pdxS"
                     /locus_tag="VYN77_001182"
     CDS             complement(18368..19243)
                     /gene="pdxS"
                     /locus_tag="VYN77_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1558647.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19395..20774)
                     /locus_tag="VYN77_001183"
     CDS             complement(19395..20774)
                     /locus_tag="VYN77_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1558648.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDFQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
BASE COUNT         6225 a         4714 c         3740 g         6280 t
ORIGIN      
        1 ctccagatta gtgaactcac ggatagttcc taatctggag atttcttatt tgcacttttc
       61 ttgtacaact ttagtccacg gtaaataggc ctctaaaacc tctttgttta cgagagtttc
      121 cacgtttgga agacattcta gaagatagga tagatatttc tcactattta taatggattg
      181 aaataagata tgaacaaatc gattaggaaa ggaaaattga tttatagaaa tgttttagca
      241 gtcgatgcgt actgttatag attcaacgca ctataattga tgacgtttag ctgtttccaa
      301 caaactcata ataatagctc ttgctttagc tccttcaaaa ctttgagaaa ataaccaccc
      361 tcgctttttt aaactgagct taggctaaaa gagtccactg gactctttta ctccgtccca
      421 taaccaatga tttaatggcg cattcagcta gattattgga aaggaccaga tgtccgtctt
      481 tcaaaatagt cttaaaggtt tctttatact tgaggctata cttgaggctg tattcaattg
      541 cccttcctag ttttgaaccc gataaaactg actgacgccg gcaccaagca aagaaatctt
      601 ccattagggg ttggagctct tcttgacgtt tctgtagtcg ttcatcagct ggcaaagcct
      661 cccagtttct ttccaaggaa aataactgat tacaataagc taaaccttta gctcctaagg
      721 atgatttatt cgcttgcttg ggggtcgctt caaaaaactt ccttctcaca tgtacccaac
      781 atccaacaag ttcagcttct tccagttgac aataagcttc tagcggttcg cagtttacca
      841 agcttagcgt cgccttgctc ctaaaccttg gactgctatc gcataggcag aactagagga
      901 ctaaagtcct aagttactgc cgcaacatat cacaatgcac atagccagaa taatctccta
      961 ggaattcttg cactaccgaa ccactccgac gctgatcatg gtggtacagc gtgattgctt
     1021 gattctcagc tttcccagac aaaaaggtcc aatagtaggt cagatggcta tcattctcta
     1081 gaacccgata ggaggtttca tctgcatgaa gtaagggctg aatcaatagt ctcgttataa
     1141 aggggcttta ggaataggag cttttacaat tttatcactt ggattattct tactgcatgt
     1201 ttggcatttg taagcgtgtt ggatatgatc cactcttttt aattgcgcag gaataaagac
     1261 caattcttgt cgttgaaggc tcgctccaat ctcttttaaa tcgtcctgac aatcagggca
     1321 aatgctctct tctacttgat gatgaacttc ttctgaatca aattgggcaa gaatagcttg
     1381 acgtttccct ttagatttct tacgtttgta ggtaatttcc tctgtttcaa ctgggaacat
     1441 ctccatcttt ctcagatgga gactcttcct caaaaaggtt gagttgttca aaagggcaaa
     1501 cacttttctc agaggatttt ccagagagct tttgcgttag ataagccact tgttcacaca
     1561 gaagggaaag ttcattagtg agactatcaa ttatagcact ctgttgttga atgattttca
     1621 atcttctccc atagactttc ctccttattt cctaacttta ttatatagtg tattgaatct
     1681 ataacagtac accttgactg ctaaaatatt tctataaatt aatttgactt tcctgataga
     1741 gatgttcata tcttatttca attcactata ctaaaaagaa agtcctcatt tcaatagaaa
     1801 tcacgacttt ctgataaatt tattttggga gtgatagaaa agcccttcat aagccaatct
     1861 acttgttcag gtgtgagagc tttgacatcc ttttctgtac tgggccaagt cagtctgccg
     1921 ttctcaaagc gtttatatag tagccaaaat ccttgaccat cccagtaaag ggctttaaag
     1981 cggtctttac gtccaccaca aaagagaaaa acttgaccgg agaaaggatc caattcaaag
     2041 tgggttttaa ccagataagc cagtgaatcg attccttgtc tcatatcagt cttcccacac
     2101 acgagataga cctgccctag gctagataga tgaattgtca tagagcaaca ccttatccaa
     2161 aagctgttct accatttcga gattgagaga ttgaaaaaaa cttacttcaa gcttgccaat
     2221 acgaatttta agcagaatat catttctctt tttagaatca aaaggacgag attgtggaat
     2281 ctctacagga acaataggtt gtggcattaa aatatcctcc aatagtttta ctttactaat
     2341 agtatacagg aggaactcca tgaatgatag ataccttgtt atgacgcgct tactatatta
     2401 gaaagttggc tctctaatta aattaattct cattatatat atcaaaagac cgcagtttga
     2461 aaactacggt ctaatttttt gtccaaatgt aaatcacttg gaactattat tcaatttatt
     2521 ctttttagta aatgttcccg ttttaatccc agttacagtt tttaatcctc tgtaaccgtt
     2581 gattttacaa agcttttaat ttttttgcga ttactcccac tcaactgttg caggtggttt
     2641 acttgtaata tcgtagacga tacggttaac atgatccact tcatttacga tacgtactga
     2701 gattttttga agtacttccc atggaatttt ggcaaaatca gcagtcatac catcgataga
     2761 agtgatagca cggattgcaa tcgtgtagtc atacgtacga ccgtcaccca taacaccgac
     2821 tgaacgaacg cctgtgttaa cagtgaagta ttgccaaata tcgcggtcaa gtccagcttt
     2881 agcgatttct tcacgaagaa tagcgtctga ttcacgaacg gtttcaagtt tctcttcagt
     2941 gatttcaccc atgacacgga tagcaagtcc tggtcctggg aatggttggc gccatacgat
     3001 atggtctggc ataccaagct ctgtaccaag agcacgaact tcatccttgt aaagagtatt
     3061 gagtggttca atcaattcaa actgcatatc ttctggaaga ccacccacgt tgtggtgtga
     3121 cttgatagtt tgagctgtat ccgtaccaga ctcgataaca tctgtatata aagtaccttg
     3181 agcaaggaat ttcacatctt tgagcttgct tgcttcgtca tcgaatacat agacaaactc
     3241 gttaccgatg attttacgtt tttgttcagg atcagaaacg ccagcaagtt tgtcaaggaa
     3301 acgtttagca gcgtctgctt tgacgatatt caaaccaaac ttaccaccga gcatgtccat
     3361 aacttgatca gcttcgcctt tacgaagaag accgtggtct acgaagatac agatcaattg
     3421 atcgccaatc gctttttgga gaagaacccc aacgacagat gagtcaacac cacctgatag
     3481 accaagaagg acacgtttat caccgacggt ttcacgaatt tttttgatct gcatgtcaat
     3541 gaaattatcc attgaccagt cacctttagc cttacaaatg ttaagggcaa agttacgaag
     3601 gatatcattt ccgtatacag aatgacgaac ttctgggtgg aattggatac cgtaaatgtg
     3661 tttatctggg ttttcgatgg ctgcgtatgg gcagtcagct gatgtacctg tacgaacaaa
     3721 gtcagcagga atctcagtaa ccgcatcacc atggctcatc aaaacagtct gttcatcagg
     3781 tgttgattca aaaagcgctg atggtgtgtg agttagggtt gattgaccgt attcacgatt
     3841 tccagcatca cctgcaggaa caacttttcc tccaagttta tgggtcaata actgcatacc
     3901 ataacagatt cccaaaattg gaattccgag ttcgaagatt tctgggtcaa tatcaaatga
     3961 accatcttca tatacagaat ttggaccacc tgatagaata attcctacag gattgacttc
     4021 acgaacttca gcagctgaaa ttttatggct ttttagttct gaaaaaacac caatctcacg
     4081 gatacggcgt gaaatcagct ggttgtactg gctaccatag tccaatacga tgattttttc
     4141 tacatcttgc aaatcagttg aaatgttgct catctttttc ctttctcaaa agaaatatct
     4201 tttcccaata ttctatcaca aataagggcg aattaccaac taaacaaggc gaagtttgac
     4261 tttttctagt ttattggggt acaatagaga aaagatagaa atgaagtgaa aaatatgcta
     4321 cctgcttata tgaaaatcca tgatcagata aaaaaggata ttgacgagca ccgttgggct
     4381 attggtgaga ggcttcccag tgaaagagat ttagctgagc agtttgcggt cagtcgcatg
     4441 accctccgcc aagccgtatc tctattagtc gaagaaggcg tcttagagcg ccgtgtagga
     4501 agcggcacct ttgtttccag tactcgagta caagaaaaga tgcgagggac aaccagtttt
     4561 actgaaattg tcaaatccca aggtaaagtt ccctctagcc agctcatttc ctacaaaaaa
     4621 accattccca atgagcaaga agttgccaag ctaggaattt ttccaacgga caatattatc
     4681 cgaatggagc gggtccgcta tgccgaccaa gttcccctag tttatgaagt tgcttctatt
     4741 cctgaaaaat tcattaagga ctttaaaaaa gaagaaatca ccagtcattt cttccaaacc
     4801 ttgcaaaaac atggctatcg tatcggcaaa tctcaacaga ccatctatgc tcgccttgct
     4861 aaagaaaaga ttgcccacta tttggaagtt gaaaaaggac atgctattct tggattgacc
     4921 caggtttcct acctagaaga tggtactgct tttgaatacg taaaaagtca atatgtaggc
     4981 gaacgctttg aattttatct tgaaaataat tagattacca cgcaaaaact ctctgtcacg
     5041 gacaaagagt cttttttatt tttctaagaa taaatctttc aaagaaatca aagttacagc
     5101 aaacaatatc attgccatac caagaaaatc aatggtataa aattgttcat tcattattaa
     5161 gaaggcaaag aaaaccgccg atattggctc aattgaagcc aacaagcttg acttgaccgg
     5221 tcctatcaga ctggctcctt taaggaaagc tgtataggca aagacagtcc cgataaggat
     5281 aatgcctgca aacgcaagga gaaaatcaag actagtcggg atatcggcct gtagaacccc
     5341 tgtaaaagga agggcgacca aacctgctat gaccattccc acaccaatga tcaagctgct
     5401 cccccacttt ttaatcaagg ctatgggtaa aatgatatac agagcataag tcaaggcaga
     5461 aaagagaccc cagaacaggc cagcaggtgt catggataac tggtccaact gcccatgtgt
     5521 tgcgatcagg aaggttcctc cgatggcgaa tatgatggaa actatctctc ccagtgtcgg
     5581 tgccacccta tccttgatac agctataaat taaaattccg acaggacaaa catactgaag
     5641 caccgtcgct gttcccgcat tggtctcctg aatagcagac agataggcga attggttgag
     5701 aaaaagacca atcagagcaa aaataagaag agacagcaaa ctctttctat cctttaaaaa
     5761 gaccagtatt ttatcctttg cagtagcata agccaagagc atgagagttc caccagcgat
     5821 taaaagacgc aagttggtca agaccagagc cgaaattccg tgtgccatca ggtattggcc
     5881 actcgttcct gacaaccccc aagcaatacc agccacaact gttagtaaag tcccttttaa
     5941 actatttgac atgtctctcc ttactcttct ttgcgaatca agttcatcac ttgattgcga
     6001 tctaatatcc attgagctct ctcgtccttt tcataggtac gattcgatag gaaaatagtg
     6061 gcttcttgct tctgacgatt ccacatgata aaggtacctg tatagcccgt atggtctagc
     6121 caatctcctt ccaaattcca tgctaaagaa cgttccttgt catccaaagg agaaaaattt
     6181 tgatttaagt ctcttgcaaa atcatctgct aaatagtgtt ctaaaaagat ttgtaaatcc
     6241 tttatagtcg aaaataaacc agcactccca gcatgtctac ccaggagacg agccttggga
     6301 tcatgcacta tgcctgcctc tacacctcta actgttggaa cagcaagctc aactggccca
     6361 aacttagttt ccgtcattcc ccaaggtttc cagacttgat cctttaaaat cacatccaaa
     6421 tcttgattaa aaattctttc caaaataaag cccaacagca aaaaatggac atccgaataa
     6481 agaaaggctg gctgacttcg tctgttgaga tgaaacatcg cttcctttaa ttcaggggct
     6541 gttaaaagat cacgattagg aataaaagga tcaaggtctg ttgcatgagt caagagctgg
     6601 cgaatagtga tgtctggata atcactctca ggtaaaaaat ctattaccag tctatcaata
     6661 tctaattgac ctatttccca caagaaggta caaactgtgc caaccccaac aaccttgctg
     6721 acactagcta ggtcataaac tagtcctgcc tcagtctgca agccatactc tgggtcactc
     6781 tggcctaaat agaactctgt ccattgattg tccttaaaat acgcaaaaga ggctccggga
     6841 taaattcctg cctcgatttg ttcttctatt tttttaataa tcttggtcca cttcatactt
     6901 cttcaaacca caattcaaca ttatttctat ctttaccaag gaagaatttt tcagacttag
     6961 gaataaaata ttcagtagac tcaaacttct ggcgaagact tgctatgtct aattcattga
     7021 ccaagaactt gagcatagat aagtcccagg taaccgtatt gtccacagtc aaatcctgcc
     7081 cctgagctgg aataaaatca agggatgccc caatttcaga tgattccaag aaactttcga
     7141 tatcagttgg gagatgtaat tccatagaaa tctcaaattt acttaaagaa attgatgcca
     7201 aatctgtttg aaattcaggc ttttctccta cttctactag acttgctctg tcatcttccg
     7261 catgaatcaa aatcaaatca tcttctggtg agaaaatttc aaaagcgtag ccattttgac
     7321 ctttatataa tcgatgaatc gaatctgttt tagataagat tccttcaatt tctaagggat
     7381 tttccacctt gacaatcaat ctagctagtt tttttcttcc ctctacctta cgagtacgca
     7441 tactgggagc ttcttctaaa accagctttt caagacccgt ttggtcacct agtgacagaa
     7501 aggccgattc ttctaacaag gccttcattc caagggtttc aatataaaat gtttcattta
     7561 attttctatt attagctttt aaagtaggaa taatccgtac aatctgattt acattcataa
     7621 attcctccgt cactttttat tttaaaggat tttagaattt tttacaagat attatgctca
     7681 aatttaacat tatttagcta tttagtaaag atagtctttc aaggattttg acaaagcctg
     7741 ataaccagca atactgaggt gcagtccatc agttgtatat tcttttttga gttggcctgc
     7801 ttggtctgtc aaacaatcaa atactggcac aaattccacc tgcatatagg cagatgcaag
     7861 ctcttgatag gcttgattcc agttctgaat tttttcattc gagcggatat agactgcctg
     7921 ctggtactcc tctccctcat tgacaggcaa aatggaaagc aatttaatct ctgtcaatgg
     7981 ataatcgcga gcaacggatt gaatgatagc ttcgagatta ttgagagcct cattcacagg
     8041 aacatccttt ccgatatcat ttgtcccaat cagaagaaaa attttatcta ctgctccacc
     8101 atatagatga gcatcaagat tctctagtaa cagtcctgtc tgatagccac gaattcctcg
     8161 attgacaatc gtctttgaag tcccaaatag ctcctgtaga ggataatact cgacaatgga
     8221 atccccaata aaaagaatgt ttggttctac aacagaaatg tgatttaggt gacgatactt
     8281 agtttgaatt ttttcttgtt cctttaggag ccaattttct aataactgta ctgccacttc
     8341 attctccttt ttctaaccag ttttccaaga cttgataaac tcctccttgg ctgttggctg
     8401 gtgctagagc agtcgccaca gctttggctt tctcatcagc attttccatc gcataggcaa
     8461 ttccagccat ttcaagcatt tcaacatcat tttcactatc accaaaagcc atgatttctt
     8521 gggatttcaa gtcccagcgc ttgagtaatt cctccaagcc ccatgcttta tgaatcccag
     8581 cttggaggat atcaatgcaa ccatagccac tggatacagc tcggacacgg ccatcaaaga
     8641 gagcattgat ttcttccaaa accgaactca aacgttcctc accaacaacc atgctcatct
     8701 tgagcacacc accaaagagg tcagatgtta attcatccac aaattgcatc cgttggtaga
     8761 atttttcaat catttctgga gtcataaaac tttcaagatc tgtaaaaatc gtaccttcct
     8821 tgacaaaatc acccttcatc cccgttacaa caaactggtc ctgacacgct cgaaccttga
     8881 aatgagtcaa agccttgttg acaatggcgt catcccatgt ctgagcctga atcaattcat
     8941 tgttttcaaa aatacgagcg ccattagcaa caaccagaac cactcgatcc accaagggac
     9001 tcagtagttg tctcatgcgg tgaatttcat tgcccgtcgc aatgacaaaa cgaatgcccc
     9061 tttgatccaa ctgatctaaa atcttttcca atcgtgggag atcaagctga cctctagcat
     9121 ccagcaaggt cccatccata tctgtcgcaa ttaccttaat catcatttac tttcctctgg
     9181 attcaagcta ataccacttc aatcccacat gttcattcat ttctttatag gctgtatcaa
     9241 ttcgttcctt agtcgcttca tctaactggt cacaatgact gccatcctct ataaaatctt
     9301 ccaaaatata agtttggtaa tgatacaagg gatatccttc tggtgaaaag gttccctttg
     9361 gtgttcgatt gacctaagta ggatgagctt tagctgttcc gatagttgtt tttccatcct
     9421 tcttcttgat ggtgacatcc atgagaacac cacgttcagt ccacttagca ttctcttcat
     9481 ctcccataga ttcaatccgt tgattggaaa tgaagttccc catttaatag ataatgagtt
     9541 tcttatttcc atctttttca accgtttcag atggttcaac aacgtgagga tgccctccaa
     9601 agataatatc cgctccccaa tcgatcatct tgtgataaag agctttttgt tcttcagttg
     9661 gttccaatcg atactcaaca cccatctgag gcatgataat ggtgatatct gcttccttct
     9721 ctgcccgttc aacttcagcc tttatcttat cttcgtttaa atctgaaaga taacgattat
     9781 agtcttcctg aaaaatatac tgctcaattc cattgaaacc ataggaatag gctaacaatg
     9841 caaccttgat agcattcact tccttaatga ccagcggagc ctgatcacgt ggttcgtgcg
     9901 tataaactcc gattggagtg attccagctt tctcaataat atcggccatt gaaataactc
     9961 cctcaatttg cgaatccaaa atatgattat gagctaaatc cagcacatga taacctgctt
    10021 ccttaatagc atccataact tcaacaggag cattaaagag aagataaccc gctaaataat
    10081 gatccttatt aatggttcct tcaaaatcac caatagctaa atctgcttgc ttgagccaag
    10141 gagtcacata ctcaaaattt tcatgaaagt cataggtacc gtcttctttt ttagctgaaa
    10201 agaaaagtcc atcgtggtag agcaaatccc cgtgggccat aattctggca gttttttcct
    10261 cttcctactc ctgagacttt tgcttagtcc cctcttgaga aatagtatct cgtttttgac
    10321 tagtcaaggg attccttgga agctttcaaa caacaagacc aagcccattg ataaaccaac
    10381 tgctagcaag agtatcgcca caaatccctt attgctccac ttgcgataac tcctaaaaag
    10441 tttaccaagc ccttcataaa acgaaaagct aaaccaccct gatttcgatt ttgtcttctt
    10501 tgtatcttca ttctccctac tttcttatgc aagccttttc tttttattat atcacagata
    10561 agtatttctt tcacaattga attgaacttc ccatctattt tctataaatc ctaaatgcca
    10621 taatgctttc aattcttgtc attttgtgat atcatgtaga ggtaatgaaa ggaaatgaac
    10681 taatccacag tggcttattc caagtatacc acttgggctt tggcagtagt taactgcact
    10741 aaacataata taaggagaga aaatgtctgc aatagaacgt attacaaaag ctgctcactt
    10801 aattgatatg aacgatatta tccgtgaagg gaatcctact ctacgcgcga ttgctgagga
    10861 agtcactttc cccctatctg accaggaaat catcctaggc gaaaagatga tgcaattcct
    10921 taaacattcc caagatcctg tcatggctga aaaaatggga ctccgcggtg gtgttggact
    10981 ggctgctccc cagttagata tctcaaaacg cattatcgct gttttggtac ctaatattgt
    11041 tgaagaaggc gaaactccac aggaagccta cgatttggaa ggcattatgt acaatccaaa
    11101 aatcgtctct cactctgttc aagatgctgc tcttggcgaa ggagaaggtt gcctgtctgt
    11161 tgaccgtaac gtgcctggct atgttgttcg ccatgcccgc gttactgttg actactttga
    11221 caaagatgga gaaaaacacc gtatcaaact caaaggctac aactccattg ttgttcagca
    11281 tgaaattgac cacattaacg gtatcatgtt ttacgatcgc atcaatgaaa aagacccatt
    11341 tgcagttaaa gatggtttac tgattcttga ataaagaaaa tcccgttgca agacggggtt
    11401 ttgtgttata atagaggcat gaaaacaaat gatattgtct atggtgtcca cgccgttacc
    11461 gaagccctcc ttgcaaatac aggaaacaaa ctctacctcc aagaagatct ccgaggtaag
    11521 aatgttgaga aagtcaagga actagctaca gaaaagaagg tgtccatttc ttggacatca
    11581 aaaaaaaatc tctctgagat gactgaaggt gctgttcatc aaggttttgt tctacgagtg
    11641 tctgaatttg cctatagcga gctagattac atccttgcaa aaacacgcca agaagaaaat
    11701 tcacttctat tgattctaga tggtctaacc gatccccata atctgggttc tatcttgcga
    11761 acagccgatg cgaccaatgt ttcaggtgtc atcattccca agcaccgtgc tgtcggagta
    11821 actcctgtcg ttgccaaaac agccacaggt gctattgaac acgttccaat tgcccgagta
    11881 accaacctca gtcaaacctt agataaactc aaggatgaag gtttctggac ctttggaacg
    11941 gatatgaacg gtactccttg ccacaagtgg aatacaaaag ggaaaatcgc cctcatcatt
    12001 ggaaatgaag gaaaaggtat ctctagcaac ataaaaaaac aggtcgatga aatgattacc
    12061 attccgatga atggacatgt tcaaagcctt aatgccagtg ttgctgcggc cattctcatg
    12121 tacgaagttt tccgaaatag actataaaaa agtttccagt catctgattg gaaacttttt
    12181 tatgattaac tatgttctgt aatgaattta taggcttctt gaccagcgat agctccatct
    12241 ccaactgctg ttgttacttg gcgaaggtct ttcaagcgaa catctccaac tgcaaagata
    12301 ccgtcgactg cagtcttcat gtggctatct gtcacaatcc agcctgcctg atcttggata
    12361 ttcaattctt taacaaaatc gctaagaggg tccaaaccaa catagataaa gacaccaccg
    12421 aaggcttgct ctgtcacttg acctgttttc acattttcaa aaacgacaga ttctactctg
    12481 ttttcaccct tgattttcct tactacagaa tcccatataa agctgatttt ctcattggca
    12541 aaggcgcgat cttgtaaaac cttttgggca cgaagttggt cacgacggtg aacaatggta
    12601 acagtcttag caaaacgagt caagaagagg gcttcttcaa cagctgaatc tccaccacca
    12661 actaccaata aatcttggtc acggaagaaa gcaccatcac acacagcaca gtaagaaaca
    12721 ccacgactgt tcagttcttc ttctccaggc actcccaaag gacggtgttt agaaccagtt
    12781 gctacgataa ctgtacgtgt ttcatatgtt tggtcatcag tcatcacttt cttaaaatca
    12841 ccatggtctt cgacattttc aacataacca taaatgtgct caacaccaag attttcaagt
    12901 ggttcaaaca tcttttcagc caattcaggt ccactaatat tagcgtatcc tgggtaattt
    12961 tcgatatcag atgtattatt catctgacca cctggcagac caccttcaat caaagctact
    13021 tttagattgc ttcgagcagc atacaaggcc gcagtcatcc ctgcaggtcc agcaccgata
    13081 ataatagtat cgtacatata gattccttct ttcttgttgt aactatcttt attctaactc
    13141 tttcttgtca atcacgcaag gattatgcct tgaaaacaag aattaaactc ataaccgcaa
    13201 aggataatac tgggacaacc aagactccaa tcataatcat atcatagaga tgggcttggc
    13261 gagccttggc tgtcttatca actcccgaca tggctctcag accaatccaa atccctaaaa
    13321 aaatcagaac aaggatggtg gtcaagatca aactctcgaa atataaagaa aatagttgca
    13381 gtagcatgat ttctctcatt tctatctttt ttaaagagta aactcagcta gtccaactaa
    13441 ctgagttttc ctttatctat tatatcaaat ataagtccgt ttgtaactag cgaagaattc
    13501 ttttgtccgc tcttctttag gggtgtggat aatctcatcc ggagttccag actcgatgat
    13561 tttcccctta tctaagaaga gaatcttatc cgcaacttgg gctacaaagg acatgtcatg
    13621 actgaccaaa atcatggtct gacctgactt agcagcatct gcaatagact tttctacttc
    13681 accgaccaat tctgggtcaa gggctgaagt tggttcgtct aagagcaaaa catctggttt
    13741 catagcaagc gcacgcgcta gggcaacccg ttgcttctgt ccacctgata aatggcgagg
    13801 ataatggttt tcacggtccg aaagcccaac cttagccaac tcttccttgg caatcttagt
    13861 cgcttcttgg tcagataatt tcttgacaac aaccaagcct tctttcacat tatcaagtgc
    13921 tgttcggcgt tcaaacaaat taaactgttg gaaaaccata gacaacttac ggcgtagggc
    13981 aaggatttct tcttgagtga ttttagaaaa atcaactgaa aaaccatcaa tctgaataga
    14041 gccactgtca ggtgtttcaa gataattgag actgcgaaga aaggttgatt ttccagctcc
    14101 tgaagaacca atcaaggcta caacttcccc tttttgaata tccaagtcca gatgatccaa
    14161 gacagtctgt cctgaaaagg atttgcttaa attcgaaatc ttaatcatta acgaaggtct
    14221 cctttcacat ctgtttgcac tgtatcaggt gcagaaatag ccatttttct ctcgatgaaa
    14281 cgaccgagac tttcaattcc gatattgact acccaataaa caagggcaac ggagatgaag
    14341 cgttcaaaat agcgataatc agctccacct agaatctgag cttgggcaaa gacttccaca
    14401 acacccgcac taaaagctag agatgttccc ttggtcaaac cgatgaggga attaatcaag
    14461 gttggagtag ctaccaccgc tgcattagga ataatcactc gtcgataaac ttgcgctcgg
    14521 gtcataccca gactgcgtgc cgcctcaatc tcaccaggat taactgagag aatggctgca
    14581 cgaatggttt cactagcata agctgcctca ttaaaggcaa aggcgacaat cgcaaaagct
    14641 gcagctggaa tcgcattgat attgagacca gttccccatt gctgattgag ggctttcaaa
    14701 gccaaaggga ttccgtagta ggtcaacatg agttgcacca aaatcggtgt cccttttaag
    14761 aaactaacaa agaaggcctg caagggatat aaaatcttga cacgattgat cttcacaatg
    14821 gcaaaaagaa gcgccaaaac caagccaaaa agggcaccac caattgtcaa cataattgtt
    14881 gttggaagtt gttggacaat tctagggatt ccatcaaaga ccgaacgtag gctaaacagc
    14941 ttgccattcg gaatcaattg catcaagttt tggtaccaat ctgatgctaa aatcgttgta
    15001 acattcataa aaacatcctc ttctgataat gattcattct accatgcttc tgccgtcaat
    15061 gaaattattt gctacggtca gatacagact ttgtctacct actagtttat catactttga
    15121 aacagggaag ttatatattt tatctatcaa agagatagat aaaaactatt tcagtcccag
    15181 ttcttcataa gattttcgat aaccgatttg cttaacggtt ccattttcta ctaaaatggg
    15241 ccgttttaac aacataccgt cacttgctag caactcagcc gcttcttggt ttgacaaact
    15301 tcctacctta tcttttagcc ctaattcacg gtatttgata ccactggtgt tgaaaaattg
    15361 cttcaattca aatcctgagg tttctagcca attcaaaatg acttcttggc taggtgtttc
    15421 ttccacgata tggacggctt tatagtccac acctaattga tttaattctt gttttgcttt
    15481 tttacaagtt gaacatttgg ggtattcgat aaattctaac atgtgcttct ccttctattg
    15541 cttctatctt taaaatctac tccttcatgc tccaagagcc aagctttctt ttccactcct
    15601 gcagcataac ctgtcagacg cttgcctgct cccaacacac gatgacaagg tactaggata
    15661 gaccaaggat tgcgtcccac tgctccacca attgcttgag cagaagccac ttgcaggtct
    15721 tgagcaattt gtccataggt cactgtctga ccataaggaa tgccctgtaa atagtcccaa
    15781 actctctttt caaaattcgt tccgattggc gccaagagca agttggataa atcctgaggc
    15841 ttgcctttaa agtaatcatc taagcaagca ataactgggt ctaaaatagg atgactaaca
    15901 acttcttcta tcgtttcatc tcctagtccc ctctcaaaat gcttctgctc ctgaacccaa
    15961 attccataca aataatggtc atcagctaca agtgatagaa taccaattgg tgaggagtag
    16021 agtattttta catacttctt ttgcattatt tctttaattt ttgataggcc aagcgttcgc
    16081 catctactgg caccttacca acttgtttaa aaccaagttt ttcaaaaata tgttgcataa
    16141 ccttgttttc agcatgcgta tctgagcgaa aatcaagata atcaaaacct tcaatcaagc
    16201 cctctaagaa ggtttgagca actccttttc cctgcacatc tgctgccaca gcaatacggt
    16261 gaaagactag atactctgac tctccagctt gccagtttcc ctcataaata gcttcatagg
    16321 cctcctctgg actcttggtc acagcagcat aggctagcag ttctccctct tccaaggcta
    16381 cgtaggcttg ccctgagatg atatcatcga taataacatc tgcatttgga tagccatttt
    16441 gccactggtc actaccagca tctgctaaac attttttggc atcctccatc acctgcatga
    16501 tggcatctac ttcatttgga aaagctaaac gaatttccat cttttttcct catttctgac
    16561 tagtttctcc catcatagca taatttaagt atttctacaa gcagttaaaa cttgactttg
    16621 tgtttttgtt attttataat ctagttatca aaactatata aaaaggagtt gaaaatgaaa
    16681 actacctttt cctacccaaa atgggcagaa attccaaaca ttgacctcta tctggaccag
    16741 gttttgctct atgtcaatca ggtctgcgcc cctatctctc ctaataaaga caagggccta
    16801 acagcatcta tggtcaacaa ttatgtgaaa aatggttacc tgacaaagcc tgacaaaaaa
    16861 aaataccaac gccaacagat tgcccgtttg attgctatca caaccctcaa gtctgtattt
    16921 tctattcaag aaatagccca gacacttaat actctacaaa ctcaagcaag ttcagaccaa
    16981 ctctacgatg cttttgtgga ctacatgaac caaggaattg acccagctaa ccctattatc
    17041 caaaccagct gccaaaccgt taaactctat catcaaactc tagacttaat cgatcatact
    17101 caagaggagg taatccaatg aacactagtc ttaaactcag caaacaactc agttttggag
    17161 aggagattgc taatagcgtg acccatgctg tgggtgcagt catcatgctt atcttgctgc
    17221 ctatttcatc catctatagt tatgaagcac acggattttt atcctctatc ggcgtttcca
    17281 ttttcgtcat cagtctcttt ctcatgttcc tatcatccac catttatcac tctatggcct
    17341 atggttcgac ccacaaatat gttttgcgaa tcattgacca ttctatgatt tacgttgcca
    17401 ttgccggctc atacacgccc gttgtcttga ccttgatgaa taactggttt ggctatctga
    17461 ttattgtcat ccaatgggga acgaccatct ttggtattct ctataaaatc tttgctaaaa
    17521 aggtcaatga gaaatttagc cttgctcttt acctgattat gggctggttg gttctggcta
    17581 tcattcctgc cattatcagt caaacgacac ccgttttctg gagtctcatg gtaactggcg
    17641 gactctgtta tacagttgga gctggatttt atgccaagaa aaaaccttat ttccacatga
    17701 tttggcatct ctttatccta actgcgtccg tacttcaata catcgctatt gtttattaca
    17761 tgtaaaaaag ttgagaaatt caatctcaac ttttttcttt acacatattg ataaagtact
    17821 ggtgcaagcg cacatcatca gtcaattctg gatgaaaaga acttaccaac atattttttt
    17881 cttgggctgc aacaatttga ttgttcactg ttgctagaat ttctacaccc tcaccaacac
    17941 tactgataat cggaccacgg ataaaggtca ttggaatctt gccaactccc ttacattctg
    18001 cttccgtgta gaaacttcct aattggcgcc cataagcatt acgctcgacc accatatcca
    18061 tagttcctag atgactctct ttctgagaag tgatttcctt agccagcaaa attaagcccg
    18121 cacaggtccc aaacactggt aagccagata gaatggcttc tcggatggga agtagcatgt
    18181 tctggtcacg taagagcttg cccatggttg tagactcacc accaggcaaa atcaaacccg
    18241 acaagtcact ctgatcttgc tgaaaatcat ctagatttct gagttctaca ctctcgacac
    18301 ctaattgatc tagcactttt gcatgttctg caaaggcccc ttgcaaggcc aatattccga
    18361 ttttcatcta ttttcctcgt tcagccatga gaatttggat ttcattttca ttaataccaa
    18421 ccatggcttc tcctaaatct tcagagattt gagctaggat ttgaggatta cggaagttag
    18481 tcacagcctt aacaatggca ctcgctcgtt taacaggatc tcctgacttg aaaatacctg
    18541 aaccgacaaa gaccccctct gcccctaatt gcatcattaa cgcagcatct gctggcgttg
    18601 caacacctcc agcagcgaaa tttacaactg gcaattttcc atgttcatga acatattgga
    18661 ccaattctac agggacttgc aaatccttgg cagcaacata aagctcgtcc tcacgtaagt
    18721 tttgaatgcg gcgaatttcc tgattcatca tacgcatatg acgaacagct tggacgatat
    18781 cccctgtccc tggttctcct ttggtacgaa tcatggaagc accttcagcg atacgacgca
    18841 aggcttcacc caaatcctta gcaccacaga caaaaggaac ttggaattct ttcttgtcca
    18901 catggaaacg gtcatcagct ggagatagaa cttcactctc gtcgatataa tcaatttcaa
    18961 tagcctctaa aatctgagct tcaacaaaat gcccgattct gaccttagcc attactggaa
    19021 tactaaccgc ttcttggatt tccttaatca tctttgggtc gctcatgcgg gaaactcctc
    19081 cagctgcacg aatatcagcc ggaattcgtt ccaaggccat cacagctgcc gcaccagcag
    19141 cttctgcgat acgagcctgt tcaggattct gcacatccat aataacacca cccttgagca
    19201 tctgtgccaa gtttttattt agttcataac gattttcagt catttgtttt atcctcatca
    19261 tttgtattgg tagctacctt ttcattttaa gtcagaacag aatgaagcac aagataaaaa
    19321 aactataatt gtatggggac agattggcta aaaactatct gaccttaact taaggccaga
    19381 tagctcattc atttttattt ttcagccgta agggcagcca ttgtgatgta gttgtatggt
    19441 ttattgaagt gtggcaagaa gaagaggtct gtcaatgcca atttatcaat tgtcacatgc
    19501 tcttggatag caagtgagaa catgtggatt cccatgctaa ttgcaatatc atgtgaaacc
    19561 atttgggcac caagaatttc acggctatct ttgtcaaaga caatcttaat tgctacttca
    19621 tggttgtcat gtttcatgaa ttctggtttt tgaaaatcgt taaagcctgt ttcagttgcg
    19681 ttgtaaccag cagctttcgc tttttcaaga gtcaaaccag ttgaaaccat gtgaagaccg
    19741 tagattgaga taccatttga accttgaaca ccgattcctt ccaattcatg tccacaagca
    19801 ttgtaggcac caacgatacc agtgcgaaca gcatttgaag caagagcgat atagcttgta
    19861 tctttacgag cattgtcata aacagtcgca cagtcaccaa cagcgtaaac gtctgggata
    19921 gatgtttctt gtttcttgtc tacaaggaag gcaccgttgc ggaagagttc gatcttacca
    19981 cctgcaaggg ctgtgtttgg acggaaacca actgcaagga taaccatatc cacgtcaaag
    20041 ctttctttgt cagtaatcaa gcgttcaact ttaccgtcac cttcgattgc tttaacagtt
    20101 tgacctagag ccaagcggat gttgtgatct tccaagttct tcgccatcat ttgtgtgaag
    20161 tctttgtcat agtaaccgtt caagacagta tcaacgatat caacaaggac aacttctttt
    20221 ccaagacgtt caaaggcttc agcaagttca acaccgatgt aaccaccacc aacaacggcg
    20281 atacggtcga ggtgttggct cttgtcagaa agtttattga taacttcttc agcattttgg
    20341 tacaatttca cgaattgtac gttttcaaga gttgctttaa attcgcggtt tcctttaaca
    20401 atttcaacac cttcgattgg tggcaagatt ggtgtagaac ctgtagcgaa aatcaatttt
    20461 tcgtatgatt ctttgtgctc ttttccttca acttccgctg taactacttt gttatcatag
    20521 tcgattgaaa gaacaggtga gttcatgtaa actttagcac ctttagcttc caatttttct
    20581 ttatcagaat agaacaagcc ttcagcaccg tcaatttgtt caccaatcca aagagccatt
    20641 ccacatccta ggaaagagat gttagagttt tggtcaaata caacaatttc gttctcattt
    20701 ccaaaattat ccaacatggt attgatacat gctgtaccag cgtggttagc accgactaca
    20761 acgattttac tcataaaaat ttcctgcctt cattctaaat ttaccttact agtataccat
    20821 ttaccgttag cgtttacaag gggggtacta ttatttcagc attttttatc aaaaataatt
    20881 ccccatttat cctgttttca agcctgatga attttatgag catatttctt gactttttcc
    20941 aaattttgtt tgtgaaaat
//
LOCUS       DAVLVZ010000031        19903 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000031 DAVLVZ010000000
VERSION     DAVLVZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19903
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.031"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..706
                     /locus_tag="VYN77_001184"
                     /pseudo
     CDS             1..706
                     /locus_tag="VYN77_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(823..1170)
                     /locus_tag="VYN77_001185"
     CDS             complement(823..1170)
                     /locus_tag="VYN77_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1558649.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(1281..3056)
                     /gene="spxB"
                     /locus_tag="VYN77_001186"
     CDS             complement(1281..3056)
                     /gene="spxB"
                     /locus_tag="VYN77_001186"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1558650.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(3263..5506)
                     /locus_tag="VYN77_001187"
     CDS             complement(3263..5506)
                     /locus_tag="VYN77_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1558651.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIRLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5516..5887)
                     /locus_tag="VYN77_001188"
     CDS             complement(5516..5887)
                     /locus_tag="VYN77_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1558652.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYIVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5898..6293)
                     /locus_tag="VYN77_001189"
     CDS             complement(5898..6293)
                     /locus_tag="VYN77_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1558653.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      6477..6575
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6659..7450
                     /gene="thiD"
                     /locus_tag="VYN77_001190"
     CDS             6659..7450
                     /gene="thiD"
                     /locus_tag="VYN77_001190"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1558654.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESIFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7660..8292)
                     /locus_tag="VYN77_001191"
     CDS             complement(7660..8292)
                     /locus_tag="VYN77_001191"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1558655.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLKKLHNILS"
     gene            complement(8285..9088)
                     /locus_tag="VYN77_001192"
     CDS             complement(8285..9088)
                     /locus_tag="VYN77_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1558656.1"
                     /translation="MQEFTNPFPIGSSSLIHCITNEISCEMLANGILALGCKPVMADD
                     SREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLVVSKNQVAVLGNGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(9088..9612)
                     /gene="thiW"
                     /locus_tag="VYN77_001193"
     CDS             complement(9088..9612)
                     /gene="thiW"
                     /locus_tag="VYN77_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1558657.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFHYSALGE
                     ILGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9617..10309)
                     /gene="tenA"
                     /locus_tag="VYN77_001194"
     CDS             complement(9617..10309)
                     /gene="tenA"
                     /locus_tag="VYN77_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1558658.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDTFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(10320..10970)
                     /locus_tag="VYN77_001195"
     CDS             complement(10320..10970)
                     /locus_tag="VYN77_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1558659.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10972..12357)
                     /locus_tag="VYN77_001196"
     CDS             complement(10972..12357)
                     /locus_tag="VYN77_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558660.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAAMAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRSLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRELTSIVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(12358..12918)
                     /locus_tag="VYN77_001197"
     CDS             complement(12358..12918)
                     /locus_tag="VYN77_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1558661.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNIFSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(13180..13268)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13477..14106)
                     /locus_tag="VYN77_001198"
     CDS             complement(13477..14106)
                     /locus_tag="VYN77_001198"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1558662.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(14108..14890)
                     /gene="thiM"
                     /locus_tag="VYN77_001199"
     CDS             complement(14108..14890)
                     /gene="thiM"
                     /locus_tag="VYN77_001199"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1558663.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKAEFINALYEITDEDVKEFKRVK"
     gene            14981..15067
                     /locus_tag="VYN77_001200"
                     /pseudo
     CDS             14981..15067
                     /locus_tag="VYN77_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(15217..15885)
                     /locus_tag="VYN77_001201"
     CDS             complement(15217..15885)
                     /locus_tag="VYN77_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1558664.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(16101..16189)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16441..17577)
                     /gene="lctO"
                     /locus_tag="VYN77_001202"
     CDS             complement(16441..17577)
                     /gene="lctO"
                     /locus_tag="VYN77_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1558665.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(18202..19692)
                     /gene="lysS"
                     /locus_tag="VYN77_001203"
     CDS             complement(18202..19692)
                     /gene="lysS"
                     /locus_tag="VYN77_001203"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1558666.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19819..>19903)
                     /locus_tag="VYN77_001204"
     CDS             complement(19819..>19903)
                     /locus_tag="VYN77_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV1558667.1"
                     /translation="PMAQQVIPKAAFGYIASGAEDTFTSFQ"
BASE COUNT         5959 a         4341 c         3703 g         5900 t
ORIGIN      
        1 aagttaggtc gttcctacac acgtgatgcc ttctatcaac tgttgaagcg ccatggttgg
       61 cgaaatatta tgccacgtcc agaacatcct aagaaagcag acgctcaaac cattgtcgcg
      121 tctaaaaata aaatctcaat tcaagaagaa aagaaagcgt tttaaaacca gtagaccttt
      181 tcataaggtt cgcttgatgt accaagatga ggctggtttc ggtagaatca gtaaactggg
      241 atcttgttgg gctccaatag gagtaggtct acatatccat agtcactata tacgagaatt
      301 tcgctattgt tatggagctg ttgatgccca tacaggcgca tcagttttct taatagctgg
      361 tggatgtaat actgagtgga tgaacgtctt tttagaagag ctttcacaag cttatccaga
      421 tgattatttt ttactcgtta tagacaatgc tatatggcat aaatcaagta ccttaaagat
      481 tccgactaat attggctttg catttattcc tccatacaca ccagagatga accccattga
      541 acaagtgtgg aaagagattc gtaaacgtgg atttaagaat aaatcctttc gaactttgga
      601 agatgtcatg aatcaactcc aagatgtcat acaaggactg gagaaggagg tgataaagtc
      661 catcgttaat cggagatgga ctagaatgct ttttgaaagc agatgagtat tatatgcaat
      721 ttctttatat aaaaagaccg gattgctccg atctttcaat agttcatatt ctcaatttct
      781 attttaaaaa tagctaaggt taacgtcaaa tgactacgcg acctatttca tacgataaaa
      841 atcaagcact agaccagcag gtccttgaac taataaggac tctgttcccc aatcggttac
      901 agttggtccg tgtaaaacct ttataccaag ctcgttcaac cgtttgtagt tctggtctac
      961 atcctcaacc tcgatatgaa taatgattcc tgactgaaag ttttccaaag gaaccaaatg
     1021 attttgtgac aacataaggc agtgactacc aatcgtaaac tgagcaaaac catcatcagc
     1081 ataatctgcc tttttatcca aaatatgctc caagtcagca cagacttggg gaacatttga
     1141 aacgataata tctaattgat ttaaattcat ttactctcct ccataaaaag accggattgc
     1201 tccgatcttt taaagttctg ctctatgaaa atcaaagaat aaagtctaca agtttcatat
     1261 ttgattttcg gcgagaggaa ttatttaatt gcgcgtgatt gcaatccttc ttcttccaag
     1321 aagagacgga atggtacgag ttcttctgct tcgtattttt ccttgaaggc tttgatagct
     1381 tcttctgagt gaagttttgg atccaattca agtacttcta ctggaagtgg acggtgttga
     1441 gtgatgcgag catcgatgac aacagtttta ccttctttgt tcaatttaac agcttctgca
     1501 acaactgcat cgatgtcttc gatacggtca actgtgaatc caacagctcc ttgagcttcc
     1561 gcaattttag cgtagtcagc gtttgtgaag tctacaccaa acaagtgttt gtttgtatct
     1621 tcgtatttgt tcttgatgaa gccgtactca gcatttgaga agacaaggtt gataactgga
     1681 aggtcgtatt gaacgtttgt gataacgtct gggtagcaca tgttgaatgc tccatcaccc
     1741 atgatgttcc atacttggcg atctggattg tctttcttag cagcgatacc accaggaagg
     1801 gcaataccca ttgtcgcaaa gagtggagat gtacgccaca tgttcttagg tgtcatgtga
     1861 aggtgacgag tagatgtttg agtagtgtta cctacgtcga ttgagtagat agcgtcttga
     1921 tcagcatgtt tgttgattgc attgtaaact tgatacaatt gcaattcacc ctcagtttta
     1981 ccttcgagtt tgttcatgta atcacgccag ttttggttgt tcttaacgtt tgcacgccac
     2041 catggagttg attcaactgg gtttactttg tcaaggatag ctttagctgc ttgaccagca
     2101 tcaccaagga ttgaagcgtc aagagcatga cgtttaccaa gtttgtaagg gtcgatatcg
     2161 acttggatga atttttcagt gttcttgaat gcttcgtaaa cttcagcgaa tgggaagttt
     2221 gaaccaagga aaagaactgt gtctgcttca aagaccactt cgttggctgg tttccaacca
     2281 acacggtaag cagaacctgt caaaccttca tagttccatt cgaaagcttc aaagttttta
     2341 ccagttgtga tgattggtgc tttgatttta cgtgacaatt cagtaatcac ttcaccagct
     2401 ttaacaccac caaatccagc atagataact gggcgttcag cattgttcaa gatttcaaca
     2461 gctttgtcga tttcaacttc gttcaaagca ggagcgatga atgagcgttc gtatgaacct
     2521 gaaccgtagt atgagttttc atcgatttct tggaaaccga agtttactgg aatttcaaca
     2581 acagctggac cttttttaga aactgcagca cggcaggctt cgtcaattac ttttggcaat
     2641 tgctcagcgt aagctacacg tttgttgtaa acagcaatac cgttgtacat tgggttttgg
     2701 ttaagctctt ggaaagcatc catgttcaat tcgttaactg gacgtgatcc aaggatcgct
     2761 aggaatggag tgttatccat agctgcatcg taaacaccgt taatcaagtg agtcgcacct
     2821 ggaccacctg aaccaactgc aaccccgatt gagccgccga atttagcttg cataaccgct
     2881 gcaagagcac ctgtctcttc gtggcgaact tgtaagaaac ggatatcttt gtcttcagcc
     2941 aaagcgtcca tcaatgagct gagtgttcct gatgggatac cgtagattgt atctacgccc
     3001 catgttttca atacgttaag cattgctgca gatgcagtaa ttttcccttg agtcataatg
     3061 ataactctcc ttcaattttt ttagacttgg agaatacgat tacatagaat tggaaacgtt
     3121 ctccaaattt ttactattcc actgtatcat atttatgctg acttttctaa aaatctgctc
     3181 aaaactctct attctctatt ctaatacagt tttgaaagtg ttgtcatttc tgttttataa
     3241 caaagaaatc tagtcattac ttttagtcta ttttactaaa atttaacaga agggaactgg
     3301 tcagaacaga tacagaacta aaggccatgg ctagacctgc caattctggg ttgagagcca
     3361 gtccaacacc tgaaaagacg cctgctgcaa tcggaattcc gacaacattg tagataaaag
     3421 cccagaaaag attgagtaga attcgatgaa aggttttctt actcatatca aaggcacgca
     3481 ccactcctaa aagattattg gttgtcaaca ccaaatctgc tgactcgatg gcgatatctg
     3541 ttccagctcc catagcaatc cccacatctg ctacactaag ggcaggagcg tcattgatcc
     3601 catcaccaac aaaggctact ttacctgact gttgcagttt atggatttca tgggcttttt
     3661 cttctggcaa gacgcctgca atgacctctt caattccgat ttgatctgca atagcacgcg
     3721 ccacaccagc attgtctcct gtcagcatga ctgttcggag accacgtttt tttagctgac
     3781 tgatggctag cttagcattt tccttaggaa tatcttgcaa agcaagcaag cctttgattt
     3841 cattgtcaac agctaagaac acaactgtct tagcttcttt ttctagttct tctagtttat
     3901 cttgataagt attagaaata tccatgccat ccagcatttt agcatttcca agtaaaactt
     3961 gttttccatt gattcgccct gaaacacctt tcccgtgcaa ggtctgaaaa ttttcaacag
     4021 tttgaaactc aagtccagct tcactcgctc gcttaacgat agcctcagcc agtgggtgtt
     4081 gagaagcatc ttccaaggag gctgccaacc caaacacttc tacttcgtcg ccgatgatat
     4141 ctgttaccac aggtttccct tccgtcaaag tcccggtctt atcaaagaca agggtttgaa
     4201 ctttctggat ttcctgtaag acagttccat ttttgaggag aacccccatc ttggcactac
     4261 gtcctgtccc caccataagg gctgtcggtg ttgcaagtcc caaggcacaa ggacaggcga
     4321 taatcaaaac cgccactccg tagagaagag aggacacaaa gctagctcca agcacgacca
     4381 cactatccct gagcaagacg aaccaaaccc aaaaggtcat gattcctaaa atgacaacta
     4441 ctgggacaaa aatccctgaa atcttatccg tcaagtcctg aatcggcgca cgacttgtct
     4501 gagctttctt cacaaaatcc acaatctgag ccaaaacagt ctctgagcca actttttctg
     4561 ctctaaagac aagcgttcca ctatgattga tggttgagcc aatgacagta tctccaactg
     4621 tcttgtccac aggcagactc tcacctgtca ccatggattc gtcaatacta gagacacctt
     4681 ctactacgac accatcaaca gcaatctttt caccgggacg cactcgaatc aggtcgccta
     4741 ccttgacttg ttccaaagga acttggacat aactatcatc actcaagact tctgcggttt
     4801 tagcttgcaa gtccagtaat ttctccacag cttgggacgt attttttctc attttttcct
     4861 caaaaactgc tcccaaaaga acgaaaaaga ggataaatcc agcactttcg aagtaaacag
     4921 ggagaccagc aaagagagca actaggctat agaaataagc cactagagtt cccagcgcaa
     4981 ccaaggtatc catgttggca ttgtgctttt taaaactggc ccaagcactc tggatatatg
     5041 gcttacctgc aactaacata ataggcgttg ttgctaaaaa ggttccccaa tgcatgactt
     5101 gatgactaat gctacctgtc aacatcccaa tcatgagaat cacaagaggc acagtaaaga
     5161 tactagtaat ccaaaaacgt tgcaggagag atagagattt tcgagtcttc tcaacgactg
     5221 tatagcttcc cttttgcatc ttcatgccac aagaaaattc atgtcgccct aattcttgag
     5281 gcgtaaaacg aatgactttc tcctcatcta cgccgattgg ttccaagata ccttcttctt
     5341 caaacagaat ttccttataa cagtttgaag gagtagcacg atgaaaggta atctcagctg
     5401 gaattccctt ttgaagccgg atatgggctg gatgatagcc tttttcagct cggatacgga
     5461 ttttttgaat gccattttct aagcttgctt tcacaatttc tgtcatagtc tccacctact
     5521 ctacaatcat cttgccgtgc atcatgttca taccacaagc aaagccaaac tctccagcct
     5581 gttcaggcgt gatttccact atatactctt cccccattgg caggttcgca tgtacaccaa
     5641 aatctggaaa aacaatttga tccagacatg gtgaaggatc cttacggtca aagacaatgc
     5701 gggctggcac tgatttcttg aggacaatca actcaggagt atagcctccc atgacttcca
     5761 ctcgaatctc ttggtatccg tttttttgct gggctttttg tccagatttt tcaggctttt
     5821 tgaaaaacca aaacaagata aatgcgataa gggcaataca aataatggtt acaatactat
     5881 ttaacatgac gtctccttta catacaatta catcttactt ctgttacagc acttgatttc
     5941 ttctctgaaa tcacagcttc caagtcttcc aagtcagtct gagtaaattc acattctaca
     6001 atcaagtcag ccaacaaatt cctaatccta cgggaacaaa ccttgtcttt gatatcttgg
     6061 acaagtaaat cccgactttg gtctagagtt aaaagggctg aataaacaaa ggacttgcct
     6121 tcttttttcc gagtcaaaca ctctttctca accagacgag ccaaaagtgt ctgaaccgtg
     6181 gacttggacc agtcaaaccg ctctgccaaa accctaatca aatctgtact ggtctgctcc
     6241 ccctgcatcc aaataatctt catgacctgc cattctgcat ctgaaatctg cattaccata
     6301 cctccaaaat ctacatttgt caattacact catcagtata ctcttaaaat ctacatttgt
     6361 caattataga aataatattt tcttcgaaaa atagaatttt aatcatttga aaaacgattt
     6421 gcagtcaaat attactatat aaacaataaa aatatgctat actaaagaaa aaagaaaaca
     6481 accactaggg gtgcgtaaag ctgagattaa cgactgttag atccctctga ctcaatctag
     6541 gtaatgctag ctgatggaag tggaaatgat aatggggact agcagtcttc tattgccttt
     6601 ctaaaacaga ctagcttgtt cttaagaata caaacttcag ttggttggga ggttttagat
     6661 gacttattta cccgttgctt tgaccattgc agggactgac cctagtggtg gtgctggcat
     6721 tatggcagat ttaaagtcat tccaagcgag agatgtctat ggaatggctg ttgtaaccag
     6781 tcttgtcgct caaaatacca gaggtgttca gctaatcgag cacgtttctc ctcaaatgtt
     6841 gaaagcccaa ttggagagta tcttttctga tattccacct caggctgtaa aaactggaat
     6901 gttggctact actgaaatca tggaaatcat ccaaccctat cttaaaaaac tggattgtcc
     6961 ctatgtcctt gatcctgtta tggttgctac aagtggagat gccttgattg actcaagtgc
     7021 tagagactat ctcaaaacaa acttactacc tctagcaact attattacgc caaatcttcc
     7081 tgaagcagaa gagattgttg gtttttcaat ccatgacccc gaagacatgc agcgtgctgg
     7141 tcgcctgatt ttaaaagaat ttggtcctca gtctgtggtt atcaaaggcg gacatctcaa
     7201 aggtggtgct aaagattttc tctttaccaa gaatgaacaa tttgtctggg aaagcccacg
     7261 aattcaaacc tgtcacaccc atggtactgg atgtaccttt gctgcagtga ttactgctga
     7321 actagccaag ggcaagagtc tttaccaggc agttgataag gccaaggcct ttatcacaaa
     7381 agctattcaa gatgcccctc aactcggtca tggttctggt ccagtcaacc atacaacttt
     7441 taaagattaa gaaaaaaaac tctctagttc ccactttaag ggaattagag agtttttata
     7501 ctcttcgaaa atctcttcaa actacgtcag cttccatctg cagcctcaaa acactgtttt
     7561 gagctgactt cgtcagtctt atctaaaacc tcaaggcagt actttgagca acctgcgact
     7621 agctttctag tttactcttt gattttcatt gagtattaat taggaaagaa tgttatgcaa
     7681 cttttttaaa aaggcttgcg tttttgcctc aatatcttct gcttgcatca aatcacgtac
     7741 aacagctaca ccagctatgc cagtgcccat aagctgatca atattctccg aagtcaagcc
     7801 tccaatagca actactggaa tggcaaccgt ttggcaaatt gttttcaagg tcgatatcag
     7861 agtaatgggc gcattttcct tggtggtggt tgggaaaatg gctcctgtac ccaagtaatc
     7921 tgcacctgat ttctccgctt ccagagctct tttaaccgtt ttagcggtga caccgaggat
     7981 tttttcagga cccaagactt tgcgagctac cgaaactggt aattcatcat ctccgatatg
     8041 cagacctgct gcatcaaccg caagacaaac atccaaccga tcatcgatta tcaagggtac
     8101 ctgataagca tctgttattt ccttgacttg ttttgccagt tgataatatt gattggttgt
     8161 gagatttttt tctcgcaatt ggactatggt aacccctgaa cggcaggccg tctcaacttt
     8221 tgcaagaaag ctttccacgg aatcttgata gcgattggtt accagatata gtctaagtgc
     8281 ttctctattc ataaacctct cctttgatgg tatctagcca attttcatct cttcttagga
     8341 gcgaaagctg attgagtact tggtaacgaa attcttccaa tcccattcct tgaacaacta
     8401 ttttctcagc agcgatattg agataagaga ctgctaagca agaagcttca aaaccagtct
     8461 ttccttggct gagaaaaaca gctgttaagg ctccaaccaa gtctcctgtc cctgttatcc
     8521 agtctaattc agtacagcca tttcccagta cagcgacctg atttttcgaa acgacgaggt
     8581 ccttgggacc tgtgactaag aaagacatac caggataggt ctgacaccag tctttcaaga
     8641 cttgaagcaa atcctccgtt tcttgatctt tagcactcgc atcgacccca acgccgtggt
     8701 gctttaatcc aacaagactt cgaatttctg acatgtttcc tttaaggacc gtaggtctat
     8761 agtctaaaag gtctttaact aagctcttac gaatggatga agtcgttacg ccaaccgcat
     8821 ctactaccat cgggagagaa gattggtttg catacgaagc tgccatgcgg attgcttttt
     8881 ccttctcagc tgacaaatgc cccaaattga tgaagagagc ctgactttgc ttagtaaaat
     8941 caagaacttc acgggaatca tctgccatga caggtttgca tcccagagcc aaaatcccat
     9001 ttgccagcat ctcacaagaa atctcattgg taatgcagtg aatgagggaa ctagagccta
     9061 taggaaaggg atttgtaaat tcctgcatca gtctatcctt tcactaaaga aatatccctg
     9121 cactttttta aagaattcct gcttgattaa aaatcgaaag gcaataaagg aaatcgctgt
     9181 accaatcaag gttgctccga aaaatcgagg cgtgtagata aaccagctaa gcttagcagc
     9241 tgatcctgta aagagtacca taacaggata ggaaacaatg gaaccaataa tacctgttcc
     9301 caaaatctct cctagagcag aatagtgaaa ttttcgaccg tacttataaa agagacctgc
     9361 tagaagggct ccaaaagtcg ctcctgtgag agctaaaggc ggaatccctt gagtcgtcat
     9421 acggataaag gctgtgactg tagccatagc caaggcataa acaggtccca tcatgattcc
     9481 tgctagaata ttgactacac tggacatcgg tgccattccc tcaattcgaa agataggtgt
     9541 aaggactaca tcaagggcaa tcatcataga taaaatggtt aatttgtgaa cttgtaattg
     9601 gtgctttctc atgcttctat tcttctcctt tttctaaaga ctgtaaatcg ctcttccatg
     9661 tctggtgttg gtaggccatt tcccaaaact tggcttccat atgaacactg atgtggaagg
     9721 tatctagcat tttttgcttg tctgtctcgt cactttctcg atagagctga ttgaccagtg
     9781 ctccctcctc tctgatctgt tgctctaact catccgtaat ataagtttca atccattgtt
     9841 gatagagagg atttggtgat ggtttaagat taagtgattt gcctatatca tggtataacc
     9901 aaggacaagg aagcaagctt gcaaaagcga tggctaagtt cggttctgca aattgcctat
     9961 aaatatgaga aatgtaatga taacaggttg gagcgattgg atgttgctcc atttcctggt
    10021 cgctgatttc caattccttg aaaaattgtt ggcgaataaa taactcaccc tccactaaac
    10081 cctgagcatt ttgtttcaag agtcttttca tctcttggtt tgaagtctta tcagccaaaa
    10141 gatgatagat ttctgagaag gccttcagat agtaggcatc ctgaatcagg taatagcgga
    10201 aaatggcagg ttctaaattc ccctcttgta attgtaaaat aaagggatga tgaaaggaag
    10261 cctgccaagc tttcttggat aattccatcg caatatctgt aaattccata ataactcctt
    10321 tataaaaata gactggtttg aagcaataaa aagaaaagca ggtagattaa ttttgttttt
    10381 ttaggaatat aaaaagtccg atagctattc ttcaactgtg catgttcgtc atatccatga
    10441 gcagacagag ctctcaggta aagatggcgc cacctaaaga ctgtcatcag aaccttactg
    10501 taaatcaagg gcgaccaaaa atgcagttct tgaccacgta ataggcaagc ttctttgagg
    10561 gacttgattt cttgctgaat gagaggaaag gaattgaata ccacaatcaa ggcataggac
    10621 caagagcgtg atagcccctt ttgagccaag tacaagagaa gctcttttag tgaaatagag
    10681 gaaacaaaga caaggccgat acaaactgtc acaaaggccc tcgttccaag catgactgcc
    10741 tgtgaagcat ctccgtgtaa ctgaactgcc cagtagttgg caaaagatgg taaaatggca
    10801 agtatgatca tccaagctaa cattttaaat cgacggtaat agagcataaa gagaatacaa
    10861 aatgcgacta ccgaaagatt cagagcaatc gaaggaatga aagatgtttc caaggataaa
    10921 atcagcaaga agagactgat aatcggtgtc tgggttgcta ctttgaccat actatctcac
    10981 ctccccttgg gtattgctac tctgagatgt aagtggtttg gtaatggtca cttctttcac
    11041 atgccgaaga ccctgactag tcatctcaat ccaataatca accacagaaa tcaaagggtc
    11101 taaacgatga ctaatgagca gaaaacttct tccttgattc ctctcctcca caatccactt
    11161 gcaaaaataa tggcaggctc tatcatccaa acctgcaaaa ggttcatcta gcaagatcac
    11221 ggaagcctta ctggtcaaga tggtcaagag ctgaagaatt ttttgctgac caccacttaa
    11281 ttgataggga ctcttatcga ctgcctgctc caaatcaaaa tatcgtaaag cttgaaaaat
    11341 ccgctgattt ctttcagaat caggtccatc taattgaagc tcctctcgca gactgactcg
    11401 gataaactgc ttctcagctt cctgaacaat actcgtcagt tcacggtaca aactcttttt
    11461 ctttttcagg actgtcccct tccatgcaat gcgcccctta tacttttgaa attgaagaat
    11521 agaccgaaag agggttgatt tcccaacacc attgtcaccc aggatacagg aaattccttg
    11581 gtaaaatgta aaatcagcaa tcgaaaagag ggagcgattt ccaaactcac aagtcgttct
    11641 atccatatgg aatagttccg ggctagaagc aacttccttt gaagcaacct gtgtcatctc
    11701 ataggaaggg atttgaaaca cttcccttag ttttccgtct cttagctcaa ccatatggtc
    11761 gatataggct ttatagtcag ataaatcatg gtcgcacaaa ataactgtct tcccatcaag
    11821 agccaactct tttagaatct ccaatatctc gattctgctc ttgcggtcaa tggaagcgaa
    11881 gggctcatcc aagagatata ccctaggatt catggcaaag aggacagcca tcgctgcttt
    11941 ttgcttttcc ccacctgata ggtgatgaat gggacggtgt aagattgcct cgcaacgaca
    12001 ttgctggaca acctctgcta ttttagaatc aatttcctga aggtgatagc cgatattctc
    12061 caaggtgaaa atcagctcct caaacaagtt ctccatggta aattgatgat tgggattttg
    12121 aaagagaatg ccaaccttct ggacacgtgc gatgatagaa agctgactga cctcgctccc
    12181 atctatcagg acttgaccac tatagggaag agaactgact tgggcaatga cttgaaaaag
    12241 gctggatttt cctgaaccac tgctcccaac taacaaggta aaggcttgcg catgaaaagt
    12301 aaaatcaaac ggctcagaga agattgggga ctgaatcgct cgtagttcca gacccatcta
    12361 tgcttttcct ccagttgcaa actgatgata gagtttgaca atggcacgaa ccaagatggt
    12421 acagaagaaa taaacagaaa taaaacgtac cacaagtaag gaaaggacaa acggaaggga
    12481 aaaggcgtag taacctaact taatgtattc atagacaaag ctaacaagcg taatcccaat
    12541 actattagca gttagagaga gccaactttc atagcgattc ttagttacga taaaaccaaa
    12601 ttcacttccc aaaccttgaa ccaagccaga caaaagagct cctagaccaa attggctacc
    12661 ataaaggact tcagcaagcg cagctagcac ttctccaatc gttgcacttc cgactctcgg
    12721 aacaaagatg gcagcaatgg gcgcagccat acaccagaga ccgaagagga tttcattggc
    12781 aaaggcctgc aaaccaagag gtgttaagag tagactgaaa atattataca catatcctga
    12841 accaacgaaa accccaccaa aaaagataga caagaaagca agcaagataa catcttttaa
    12901 ctgccatttt ttcaacataa aaaactcctt ttttaaaaga aaagtggggc actcaagaag
    12961 accgacctaa atactttgta tagcagactg aatttagaac agtacacaag aatgctaaaa
    13021 tatttctaga aattaatttg aattttctaa ttgatttgtt cgcatcttat ttcaatctac
    13081 tatatcatct tcatccagtt tcgtaaaaga aaaaactcta attacagata cgaattagag
    13141 ttcagcttac aagattagac agttcttttc gacatacgaa aaaaacattt cacatttccc
    13201 ttcgccagtc ttaactgtat caggttcaat gggtatcatc tcagcctaaa gcaccccaaa
    13261 tgtctttatt atttaattat gtgattatta taacacacat tttatactag ttcaagaaat
    13321 tgaactggaa atacagcctt gcactcacaa agacagcaga tctttctttt gcaaaaaaca
    13381 aatgacctgt ttgatgaatt agccattcaa gctgaatctg gacatagctt tttaaaaaag
    13441 gaaaatccta cttacttaga atccaaggat agatatctat tgttcactca tttcccgaac
    13501 agttttttct atattttttg catacgatat tgccgaaatg attgaaacgc catccatatt
    13561 ggtctttata atgtctttaa tatgtttcgt ctgtatccca ccaattgcaa ctaaaggcat
    13621 ttgtggcaat agttttctca tcaattcaag accttcataa cctatagcac caccagcatc
    13681 atcttttgac tgggtatcaa atacaggccc aacacctaca taatctacat attcaacttt
    13741 tgattgttga aattcttctt cgtttcttat agaaagacca attattttat ctggcatcaa
    13801 ttttctaatt tcatcaacac caatatcatc ttgacctaca tgtacgccat cggcgtcaat
    13861 ttccattgct aaatctatat cgtcattaac gataaatgga acattgtatt ttttacaaag
    13921 ttctttaatt tggatagcta gctcaagttt ttctaagcct tctaaagcac cctcaccttt
    13981 ttctcgaaat tgaaataagg ttataccacc ttttaaggct tcctcaacga ctgtatatag
    14041 attttttcct tggcaagtag tcgttccaca aataaaatat agttttagta attctttatg
    14101 aaacatctta cttcactctt ttgaattcct ttacatcttc atctgtaatc tcgtataagg
    14161 catttataaa ttcagcttta aatgttccag gaagatgtcc attgggacgt ttttctgcca
    14221 tttctccagc gatattgtaa actaacattg ctgtttctaa tgattttaat tcttgacctt
    14281 tttccagtcc gataaaactt gctactacag ctcctaataa gcatcctgtc ccaatgactt
    14341 tcggcatcat agcactacca ttatgaatcg ttaccacttc tccattaaca gcaatggcat
    14401 ccacttcacc tgttactact attggaatat tgaacttctc atttgctgct agagcaattt
    14461 cgtcaatatt atctacgccc gcactatcta ctcctttaga tgccacatct attcctacta
    14521 aagaggcaat ctcgccagca tttcctctaa tcgctgctag tttataattg ttgattagat
    14581 catctgctac tttttttcta tattctcctg ctccacaggc tacaggatct aaaactgctg
    14641 ggacattata tttctccgca attttcagag cagcttggta taatttccaa ttttcatctg
    14701 tcaatgttcc tatgtttatt aataaaccac cagcatactt taacaaatcc tctaaatctg
    14761 ctggaaactc actcatggct ggtgaggcgc ccagtgctac taatccattt gctgtgaaat
    14821 tttttactac atcattggtt atacaaatga ccaatggtgc tttttctttt aataatttta
    14881 aacttgtcat attgaaatcc ttccttttca ctttatacga tctactgatt tcgatttatc
    14941 tctatctttc gttaagaaat ttttttcatt tacgttgaat gatttatact caatgaaaat
    15001 caaagagcaa actaggaagc taaccgcaga ttgctcaaaa cactgctttg aacctgtaga
    15061 taagactgac gaagtcagct caaaacactg ttttgaggtt gtggatagaa ctgacgaagt
    15121 cagtaaccat acctacggca aggtgaaact gacgtggttt gaagagattt tcgaagagtc
    15181 ttacctcatc aaatttgtaa atatcatgag ccttctctag acatcgtaac caatatcaaa
    15241 aaaagctaat tctaaagcga ctgcttgatt ccagcgttgc tgaagttctg tcaaatcttc
    15301 tcgattttta ccgacacgat tgagttcgtc aaccagaaat tgaacccact ctgcaaagaa
    15361 aggacctctg tggagattga tccattccga atgaatatag acttcaggta gagccaaatc
    15421 tttagaaccc cagtctaaat agagaccttc tgcaatgacc agcataacca aaagatgggc
    15481 atagtctgat gaagccaccg ccgaatacat tagatcctga aaggcttttg ttacagggtg
    15541 caaagtcact tctaggtagt cattctctgc tacttttaac tctttaaaag ccttttggaa
    15601 ataaccatct tcatctgctt caagaaagcc tagttgcttg gcaaaacgaa gcttggattc
    15661 aagtttatct gcgtgagcta cgcaggcacc cagcatggat aagaaggcat caaagaagtg
    15721 ataatcttga atcagatagt cctttaagac cttattctca attgtccccg caaaaagttc
    15781 cttaacaaaa cgatgattga ttgcagcctg ccaatccttc tgactgcttt ttaataattc
    15841 tccaacagtc aaacctggct gaaatgcata gtcttgtgtt tccatattta cttctcctct
    15901 ctttacttgt tagtaattaa taaaacacca agaaatatca agcaaaatcg taattccact
    15961 tgatcctttt aaagcacatc gagagcattt gcagagaagc taactaaaca agcctatcca
    16021 gtttatataa acaaaaaact ccaattacaa tcaagaatta gagttgactt acaagattag
    16081 atcgttcatt tcgccatacg aaaaaacgtt tcacatttcc cttcgccagt cttaactgta
    16141 tcaggttcaa tgggtatcat ctcagcttaa agcaccccaa atgtctctat tatttaatta
    16201 ctgaaccagt atagcaaaaa atgaaagccc tagcaagata tttgaccgaa aaatatcttt
    16261 atatagtaga ttgaaactag aatagtacac ctctacttct aaaacattgt tagaaatcga
    16321 tttgactgtc ctgattgatt tgtcctattc ttatttcatt ttactatagt tttcgatagc
    16381 aatttattct tccaatacac gaagaaaaac ctccacattc agtggaggca atctgtttta
    16441 tcaatacaat tttaagtcac gagggtcaac tgggaaggtt gggttgtatg gattgtgacg
    16501 gagcttaaag tgtttgacat cttcaatggt ctgagctcca gacaattgca taactgtctt
    16561 caattccgca ttcaagtgtt caaagacttg acgcacaccg acactaccac cgagagccaa
    16621 gccataaata acagggcgac caatagctac caagtctgct cctgaagcca aggctttaaa
    16681 gacgtgttga ccacgacgaa caccagagtc aaagacaatc ggcacacgtc tatcaactgc
    16741 ttctgccact tcttgaagcg agtcaaaggc agctggtcca ccgtcgattt gacgaccacc
    16801 gtggttggtt acccagatac cagaagctcc tgcagcaagt gaacgttcaa cgtcctcacg
    16861 gcattgtggt cccttgacat acacaggaag accagagtat tcagcgataa attctacatc
    16921 gcgtggagac aagcgttgtt tagctgattt gtaaacaaag tccattgatt taccagcacc
    16981 ttctggcagg tattcttcaa caatcggcat gccaactggg aagacaaaac cattacgctt
    17041 atccacttca cgattccccc ctacagtagc atctgccgtc aagacaatcg ctttataacc
    17101 ttcagccttc acacggtcca tgatgtggcg gttgataccg tcatccttac taaagtaaaa
    17161 ttggaaccaa tggggtgtcc cttgaagggc ttcagaaatc tctggaaggt caacagtaga
    17221 gtaagagctg gttgtataaa gagaaccaaa ctcatgcaca ccacgcgcag tcgccacttc
    17281 cccctgttca tttgccaatt tatgagccgc aacaggcgcc ataatgattg gtgaagatag
    17341 tttttcacct gcaaattcaa tctctgtact tggattttca acatcgcaaa gtgtatgagg
    17401 aacgatgagt ttgtggttaa aggcgcggat attctctctt aaagtgaaag tatcttccgc
    17461 cccactagcg atatagccaa atgctgcttt aggaataact tgttgcgcca ttggctccaa
    17521 atcataggta ttgatgaagt ctacatgacc ttctgcattg cttgttttgt atgacataaa
    17581 atgccctcct tgattaagta agcgtttgct ttttactaca caactataat aactcttttt
    17641 tcaatcattt tcaagagttt tgcttaaaaa ccatcttatt ttttcataca atatactagc
    17701 cctactttcc ttaaactcta aactttagtc tatattaagt gaatcgttga gattttttgc
    17761 tgccaggcat ttgaactact tttttatttg cggattctat ctactaatac tataaaaaaa
    17821 caagtgaata agacaccaga acatcctcta ataggaccta tggacttata tcagtcccat
    17881 atatactgtt taagcaagat actatcctag ttgacgctaa cttcctatcc cacttattaa
    17941 ttttttattt atagtgaatc tgaacaaatc aattggggaa tccaagtcaa tttctagtac
    18001 tttttgaaca gtttcagcgt actatcgtaa aatgaaataa aacatgcaca aattgcttag
    18061 ggaatttaaa ccaatttcta acaatgtctt agaaatcaaa gtgtactatt tcaacttcaa
    18121 tatactacac ttcattttct ccttaaattc agtctctttt tgaatcaaaa aatcccctgg
    18181 taagagccag aggataagaa tttatttcat tgttgggaag agcaatacat cacggatagt
    18241 tgttgtatca gtgaggagca tgcagagacg gtcgataccg attcccaaac cacctgttgg
    18301 tggcataccg tattcaagag cttcaatgta gtcatagtcg attcctgtcg cttcatcatc
    18361 accaagttct ttggctttag cttgggcttc aaaacggcta agttggtcga ttgggtcgtt
    18421 caactcagta aaggcattac cgtactcctt agtcatgata aagagctcga aacggtcagt
    18481 aaagcgttgg tcttcaggat ttttcttagc gagtggagat acagctactg gatgtccata
    18541 gacaaaggtt ggttggatta aagtttcttc aacaaactct tcaaagaagg cattgatgat
    18601 gtgaccaacc tcagtgtagt gtttctcaac tggaactttc ttctcagcag cgatagcttt
    18661 agcttcttct aaagtcatgt cttgccagaa atcgacacca gtaatttctc tgatagcatc
    18721 caccatatga acacgcttaa atggttcgtt aatcttgatt tcagtacctt ggtagttgac
    18781 tgggccatcg cccttaactg atttagcagc gtgttggata atgccttccg tcaagtccat
    18841 gatgtcttgg aagtctgcat aagcttggta aacttcgata gaagtaaact cagggttatg
    18901 agtagcgtcc attccttcgt tacggaagat acggccaatt tcatagacac gttccatacc
    18961 acccacgata aggcgtttta agtgaagctc agtcgcgata cgaagcacca tgtcaatgtt
    19021 ttgggcattg tggtgggtga taaatggacg ggcagcagca ccaccggctt cattatgaag
    19081 aacaggtgtt tccacttcaa ggaatccttt ttggtcaagg taacgacgga tttcagagat
    19141 gatttttgaa cgagtgacaa agcgttcaaa gctttcacgg ttagaaatca agtcaaggta
    19201 acgtttacgg taaattgttt caacgtctgt caaaccatgg aatttctcag gaagaggacg
    19261 aagagcctta gacaagtgtg tgatgtgggt tgccttgata gagagttctc ccatatccgt
    19321 acgcatcact tcaccttcga caccaaggaa gtcaccaagg tctgcttttt tgaagatttc
    19381 gtagttttct tcaccgacag catccttacg aacgtagatc tgaatctggc cttcgcggtc
    19441 ttgaaggtgg gcaaaaccaa cttttccttt accacgtttg gttaccaagc gtcctgcgat
    19501 agtagctgtt tcgtttttat cgtgtaattg ttctttatcg aggttggcat atttatcttt
    19561 taattcttgt gaatttgcag tacgttcaaa acgttttccg aaaggatcga ttccttgttc
    19621 gcggagcgca gccatttttt cacggcgaac gatctgctgg tcatttagtt cttccatatg
    19681 ttctgtagac atgggatcct cctagtttta ctcaaaattg aatacgatga gtcatttttt
    19741 gcgatacccc attttatcat aaataagcaa gaaattcacg gataatcttt aaaaactaaa
    19801 aagaacctga cgctaaaatc actggaaaga agtgaaagta tcttccgccc cactagcgat
    19861 atagccaaat gctgctttag gaataacttg ttgcgccatt ggc
//
LOCUS       DAVLVZ010000032        19836 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000032 DAVLVZ010000000
VERSION     DAVLVZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19836
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.032"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            132..3233
                     /locus_tag="VYN77_001205"
     CDS             132..3233
                     /locus_tag="VYN77_001205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1558668.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLIGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3316..4323
                     /gene="pfkA"
                     /locus_tag="VYN77_001206"
     CDS             3316..4323
                     /gene="pfkA"
                     /locus_tag="VYN77_001206"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1558669.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEVIIPEADFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4382..5887
                     /gene="pyk"
                     /locus_tag="VYN77_001207"
     CDS             4382..5887
                     /gene="pyk"
                     /locus_tag="VYN77_001207"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1558670.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDETREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6550..6772)
                     /locus_tag="VYN77_001208"
                     /pseudo
     CDS             complement(6550..6772)
                     /locus_tag="VYN77_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7620..8492
                     /locus_tag="VYN77_001209"
     CDS             7620..8492
                     /locus_tag="VYN77_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1558671.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLDTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8641..8982)
                     /locus_tag="VYN77_001210"
                     /pseudo
     CDS             complement(8641..8982)
                     /locus_tag="VYN77_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9125..9436
                     /locus_tag="VYN77_001211"
     CDS             9125..9436
                     /locus_tag="VYN77_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558672.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            9440..10156
                     /locus_tag="VYN77_001212"
     CDS             9440..10156
                     /locus_tag="VYN77_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1558673.1"
                     /translation="MKKYFIGGLGNNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10286..11101)
                     /locus_tag="VYN77_001213"
     CDS             complement(10286..11101)
                     /locus_tag="VYN77_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1558674.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11098..12003)
                     /locus_tag="VYN77_001214"
     CDS             complement(11098..12003)
                     /locus_tag="VYN77_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1558675.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMFFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            12415..14544
                     /locus_tag="VYN77_001215"
     CDS             12415..14544
                     /locus_tag="VYN77_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1558676.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14531..14969
                     /locus_tag="VYN77_001216"
                     /pseudo
     CDS             14531..14969
                     /locus_tag="VYN77_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
     gene            15027..15299
                     /locus_tag="VYN77_001217"
     CDS             15027..15299
                     /locus_tag="VYN77_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1558677.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15653..15844)
                     /locus_tag="VYN77_001218"
     CDS             complement(15653..15844)
                     /locus_tag="VYN77_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558678.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16272..17030
                     /locus_tag="VYN77_001219"
     CDS             16272..17030
                     /locus_tag="VYN77_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558679.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            17032..19020
                     /locus_tag="VYN77_001220"
     CDS             17032..19020
                     /locus_tag="VYN77_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1558680.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSAFFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTIHKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19062..19706)
                     /locus_tag="VYN77_001221"
     CDS             complement(19062..19706)
                     /locus_tag="VYN77_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1558681.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(19732..>19836)
                     /locus_tag="VYN77_001222"
     CDS             complement(19732..>19836)
                     /locus_tag="VYN77_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1558682.1"
                     /translation="NNYPKKCLDYKSLREDFWMANLNLKFSVRNKSRN"
BASE COUNT         6100 a         3603 c         4204 g         5929 t
ORIGIN      
        1 atataaaaat gatgacaaaa atacaaatga atagaaaata aattagtaag ctgatgaaat
       61 ttttctcaag agaagccatt tataggtgaa aatggtataa tatagtgaga aggatagagg
      121 agaagtgtaa attgatcgca caactagata caaaaacagt ctatagtttt atggaaagcg
      181 tcatttcgat cgaaaagtat gtgagagcag ctaaagaata cggctacact catttggcta
      241 tgatggatat tgacaatctt tatggcgctt tcgactttct agagattaca aaaaaatacg
      301 gcattcatcc tttgataggg cttgaaatga cagtgtttgt agatgatcag gaagtgaatt
      361 tgcgcttttt agctctatct agtgtgggct atcagcagtt gatgaagctt tcgacagcca
      421 agatgcaggg ggagaaaact tggtcagtcc tgtcccagta cctggaggat atcgcggtca
      481 ttgtgcctta ttttgataga gttgagtcgt tagaactagg ctgtgattac tatatagggg
      541 tttatccaga aacactagca agcgaatttc atcatcctat cttacctctt tatcgggtca
      601 acgcttttga aagcagggat agagaagttc ttcaagtttt aacagcgatt aaagaaaatc
      661 taccgctcag agaagttccc ttgcgttcga gacaagatgt ctttatatca gcaagttctt
      721 tagagaaact attccaagag tgttttccgc aagctttgga caatttagaa aagcttattt
      781 caggcatttc ttacgacttg gatactagtc tgaaactgcc tcgttttaat ccagctagac
      841 cagcagtaga ggagttgaga gagcgtgctg aactggggct tgttcagaag gggttgacta
      901 gtaaagaata tcaagataga ctagaccaag aattgtctgt tattcatgat atgggctttg
      961 atgattattt cttggttgtt tgggatttgt tgcgttttgg acgatcgaat ggctattata
     1021 tgggaatggg aaggggttct gcagtaggca gtttggtttc ttatgcctta gacatcacgg
     1081 ggattgaccc agtagagaaa aatctgattt ttgaacgctt tcttaatcgt gaacgctata
     1141 ccatgcctga tattgatatt gatatcccag atatttatcg tccagatttt atcagatatg
     1201 ttggtaataa atatggtagt aaacatgcgg cacaaatcgt tactttttca acctttggag
     1261 ccaagcaagc tcttcgagat gtcttgaaac gctttggtgt gccagagtat gaattatctg
     1321 caattactaa gaaaatcagt tttcgtgaca atcttaagtc ggcctatgag ggcaatctcc
     1381 agtttcgtca gcaaatcaat agtaagttag aataccaaaa agcttttgag attgcttgca
     1441 agatagaggg ctatccaagg caaacctctg tccatgcggc tggtgttgta attagtgacc
     1501 aagatttaac caactacatt cctctaaagt atggtgatga aattccactg actcagtatg
     1561 atgctcatgg agttgaggct agcggacttt tgaagatgga ctttctggga ctacgaaatt
     1621 tgacctttgt ccagaagatg caagagttgc ttgctgaaac agaaggtatt catctgaaaa
     1681 ttgaagaaat cgatttagaa gacaaagaaa cgttagcttt atttgcctct ggtaatacaa
     1741 aaggtatctt tcaatttgag caaccaggtg ccattcgtct gcttaagcgt gtgcaaccag
     1801 tctgttttga agatgtcgtc gcgactactt ctctaaatcg accgggtgct agtgactata
     1861 tcaataattt tgtggcaaga aagcatgggc aggaagaagt gactgttctg gatccagtac
     1921 tggaggatat tttggctcca acctacggca taatgctcta tcaggagcag gttatgcagg
     1981 ttgcccagcg acttgccgga tttagtcttg ggaaagccga tattttgcgt cgggctatgg
     2041 ggaaaaagga tgcctctgcc atgcatgaga tgagggcttc ctttattcaa ggttcattag
     2101 aagctggtca tactgtggaa aaagcagagc aggtctttga tgttatggag aagtttgcag
     2161 gctatggttt taacaggtca cacgcctatg cctactcagc attggccttc cagttggctt
     2221 attttaaaac acattatcca gccatatttt atcagattat gttgaattct gccaacagtg
     2281 attacttaat agatgcactt gaagcaggtt ttgaagtagc ctctctatcc atcaacacca
     2341 ttccctatca cgataaaatt gccaacaagg ccatctatct aggtttgaaa tccattaaag
     2401 gagtcagtaa tgatttagct ctctggatta ttgaaaatag accttattct aacattgaag
     2461 attttatagc taaattacct gagaattatc tgaaacttcc tctgctagaa cctttggtaa
     2521 aagttggtct tttcgattca tttgaaaaaa atcgtcaaaa agtatttaat aacttagcta
     2581 atctatttga atttgtgaaa gagttgggaa gtttgtttgg agatgctatt tatagttggc
     2641 aggaatcgga agattggacg gaacaagaaa aattttatat ggaacaagag cttttaggga
     2701 taggtgtcag caaacatcca ctacaagcta ttgcaagtaa ggctatttac ccgattaccc
     2761 caatcggaaa tttgtcagaa aatagctatg ctattatttt ggttgaagtt cagaaaataa
     2821 aagtgattcg taccaaaaag ggtgaaaata tggccttctt acaggcagat gatagtaaga
     2881 aaaaattgga tgtcactctc ttttcagact tatatcgtca ggttggacag gaaataaaag
     2941 agggagcctt ctactatgta aaaggaaaaa tacaatcacg tgatggccgt ctgcaaatga
     3001 ttgcacaaga aataagagaa gcagttgctg aacgcttttg gatacaggtg aaaaatcatg
     3061 aatcggatca agaaatttca cgcattttag aacaatttaa aggcccaatc ccagtcatca
     3121 tccggtatga agaggaacag aaaaccatcg tttctcccca tcattttgta gctaaatcca
     3181 atgaattaga ggagaaattg aatgaaatcg ttatgaaaac gatttatcgc taaaaatacg
     3241 gaaaatagaa gaattttaaa atcaaatgtg atataatcaa gaagaatgtt aaaagaaaaa
     3301 ggagcataaa ccaatatgaa acgtattgct gttttgacta gtggcggaga cgcacctggt
     3361 atgaacgctg ccatccgtgc agttgttcgt caagcaattt cagaaggaat ggaagtgttt
     3421 ggtatctatg acggatatgc tggtatggtt gccggtgaaa ttcatcccct agatgcagct
     3481 tcagtagggg acatcatttc tcgtggtggt acttttcttc actcagctcg ttatccagag
     3541 ttcgctcaac ttgaagggca acttaaaggg attgagcaat tgaaaaaaca cggaattgaa
     3601 ggtgtagttg ttatcggtgg tgacggatct taccacggcg ctatgcgttt gactgaacat
     3661 ggcttcccag ctattggtct tccaggtaca atcgataacg atatcgttgg tactgacttt
     3721 acaatcggtt ttgacacagc ggttactact gccatggacg ctattgataa gattcgtgat
     3781 acatcatcaa gtcaccgtcg tacttttgta atcgaagtta tgggacgtaa cgctggtgat
     3841 atcgctcttt gggctggtat tgcaactggt gctgatgaag tcatcatccc tgaagcagac
     3901 ttcaagatgg aagatatcgt agcaagcatc aaagctggtt atgaatgtgg taaaaaacac
     3961 aatatcatcg tcttagctga aggtgtgatg tcagcggctg aatttggtca aaaacttaaa
     4021 gaagctggag atacaagcga ccttcgtgta acagaacttg gacatattca acgtggtggt
     4081 tctccaactg cgcgtgaccg tgttttggcg tcacgtatgg gtgcacatgc tgttaaactt
     4141 cttaaagaag gtatcggtgg tgttgcggtt ggtattcgta acgaaaaaat ggttgaaaat
     4201 ccaattcttg gtactgcaga agaaggggca ttgtttagcc ttactgcaga aggtaagatt
     4261 gtggttaaca acccacacaa agccgatatt gagctatcta gcttgaataa gagcttgtca
     4321 taatttacaa tttagttaat aagaccaaaa aggtcatata tataaaggag tcacaaaaat
     4381 catgaacaaa cgtgtaaaaa tcgttgcaac tttgggtcct gcggtagaaa tccgtggtgg
     4441 taaaaaattc ggtgaggacg gatactgggg tgaaaaactt gatgttgaag cttcagctaa
     4501 aaacattgct aaattgattg aagctggtgc taacacattc cgattcaact tctcacacgg
     4561 cgaccaccaa gaacaaggtg agcgtatggc aactgttaaa cttgcggaaa aaattgcagg
     4621 taaaaaagtt ggtttccttc ttgatacaaa aggacctgaa atccgtacag aattgttcga
     4681 aggtgaagct aaagaatatt catacaaaac tggtgaaaaa attcgtgttg caactaaaca
     4741 aggaatcaaa tcaactcgtg aagtgattgc gttgaacgtt gctggtgctc ttgatatcta
     4801 tgatgatgtt gaagttggtc gtcaagtttt ggttgacgat ggtaaacttg gtcttcgtgt
     4861 ggttgctaaa gatgatgaaa ctcgtgaatt tgaagttgaa gttgaaaacg atggtatcat
     4921 cgctaaacaa aaaggtgtga acatccctaa cactaaaatt cctttcccag ctcttgctga
     4981 acgcgataac gacgatatcc gtttcggtct tgaacaaggt atcaacttca tcgcaatttc
     5041 attcgtacgt actgcaaaag atgtgaacga agttcgtgca atctgtgaag aaactggaaa
     5101 cggacatgtt caattgttcg ctaaaatcga aaaccaacaa ggtatcgata acttagacga
     5161 aatcatcgaa gcagctgatg gtattatgat tgctcgtggt gatatgggta tcgaagtacc
     5221 gttcgaaatg gttccagttt atcaaaaaat gattatcaag aaagtcaatg ctgcaggtaa
     5281 agttgttatc actgcaacaa acatgcttga aacaatgact gaaaaaccac gtgcaactcg
     5341 ttcagaagta tcagacgtat tcaacgctgt tatcgacgga actgacgcta caatgctgtc
     5401 aggcgagtct gcaaacggta aatacccact cgagtcagta actacaatgg ctacaatcga
     5461 caagaacgct caagctcttc ttaatgaata cggacgtctt gattcagatt catttgagcg
     5521 taactctaag acagaagtaa tggcttctgc tgttaaagat gctactagct caatggatat
     5581 caaattggtt gtaactctta ctaagacagg tcatactgca cgtttgattt ctaaataccg
     5641 tccaaatgct gacatcttag cattgacatt tgacgaattg acagaacgtg gcttgatgtt
     5701 gaactggggt gttatcccaa tgttgacaga tgctccatct tcaactgacg atatgttcga
     5761 aatcgctgaa cgtaaagcgg tagaagcagg tctcgttgag tcaggcgatg atatcgttat
     5821 cgttgctggt gtgccagtag gagaagctgt tcgcacaaac acaatgcgta tccgcacagt
     5881 acgttaagaa aaatataaaa acctatcata tccagctttc tagcctgtat gatgggctct
     5941 ttttttgtcc agggggcaaa actttcataa tggattgata ctcttcgaaa atctcttcaa
     6001 accacgtcag cgtcgcctta ccgtactcaa gtacagcttg cggctagctt cctagtttgc
     6061 tctttgattt tcattgagta tgaaataaga tatgcacaaa ttgattagaa agtcaaatga
     6121 atttctacaa atgttttagc aatcgtaatg tacttgtcta gattcgatct gatatatttt
     6181 cgatttaatg atatggtatt taaaaccttc aaagtagctt gctccattct tttacttacg
     6241 tgagtgtaga tgttatttac tgttttagcg tttttgtgtt ccactctaac cattatagca
     6301 ttcttctcag ctagtgtact aaggagtgtg tgcctgaaaa tatgggaact aaggggctgg
     6361 tttatcggtt tctctagttt agtatttgcc ttttgcaaag tgatgttaaa tgcctttctc
     6421 taaatttaca tatcactatt gtttaacaaa atctaatcta ttttaggtca cttattcttt
     6481 ttttgaaatg tagaatgaac tttttcaaag tttttcgaat cttttaaaat ctgtttgttt
     6541 tatatcgcca ttctccctat atagcctgac aactttcccg atggtacgaa tatggttgct
     6601 ttcgtctagg tggatgtcgg ggtatttggg attgagtttt tttaaggtag ccttggcgga
     6661 gtttcttgac atagttagcg ccgtctactt ggaagatgcc gatggtatta tagtcaatct
     6721 gtggggtatt cttgataaat aggtagtcgc tgtttcttat ctttgcctcc atggacttgc
     6781 tgacgacata agcgattggg tcgtagtcgt ctgggataat ggaaactcca tatctaaatc
     6841 gttgtcctgc atcgagcggc tacctgcaga gataaactac ctaacacgag agtaagtagt
     6901 ctgtctgtag tcgtccagtc tgatgatttt tacgatactt cgtttttctg atcatacagt
     6961 tgcctctcgg cataggtcag aactttacct tgtctgggtg gtgcccgttg gtcgtagata
     7021 gattggatat cgctaggaga atccttttga actggagaaa agagggcatc aatcaagcta
     7081 ctgaatactt taactaagtc aaatatagta tttttcttag tagacctaac ccttttttca
     7141 taatttccaa tggtgttttt acttatacca atcttagtac ccaattctta ttgagtccaa
     7201 ccattactag tctatattgt tttatagttg attgagtttg gaatagtacg ctgtagctgc
     7261 taaaacattt ctaggaatta atttgccttt cctaatagag ttgttcatat cttatttcaa
     7321 tctactatat ttttcgcctc taacaatcgc aatctcttct ttatctacga atgaaatcgc
     7381 tttctatttt tgtaagtaaa gcatcacacg aaatccacga aaatgaaaac ctttgttgtg
     7441 ttttcgtaaa aaatttgttg acagagcacg aaacgcctac tataattaaa tcaagcttaa
     7501 ggaaataaca aaaaaacaaa tcggagggaa agacgatgaa catattaaat gagaaagtaa
     7561 tcaatatctg taaagcagta gttaaagaaa ctttaatcca agacatttag gagaaagaaa
     7621 tgaagaaatc aagaaaatta gccacattag gaatctgttc agctttgttt ttaggcttgg
     7681 ctgcttgtca acaacaacat gctacttctg aggggacgaa tcaaaggcaa agcagttcag
     7741 cgaaagttcc atggaaagct tcatacacca acctaaacaa ccaggtaagt acagaagagg
     7801 tcaaatctct cttatcagct cacttggatc caaatagtgt tgatgcattt tttaatctcg
     7861 ttaatgacta taataccatt gtcggctcaa ctggcttatc aggagatttc acttccttta
     7921 ctcacaccga atacgatgtt gagaaaatca gtcatctctg gaatcaaaag aagggcgatt
     7981 ttgttgggac caactgccgt atcaatagtt attgtctttt gaaaaattca gtcaccattc
     8041 caaagcttga aaagaatgac cagttgcttt tcctagataa tgatgcgatt gataaaggaa
     8101 aggtctttga ttcacaagat aaggaagagt ttgatattct attttcgaga gttccaactg
     8161 aggcaactac agatgtcaag gttcacgctg aaaagatgga aacattcttc tcacaatttc
     8221 aattcaatga aaaagctcga atgctgtctg tagtcttgca cgacaatctg gatggcgagt
     8281 atctgtttgt aggccacgtt ggggtcttag tacctgctga tgacggtttc ttatttgtag
     8341 agaaattgac tttcgaagag ccctaccaag cgattaaatt tgctagtaag gaagattgct
     8401 acaagtattt ggacaccaag tatgcggatt atacaggcga gggactggct aagcctttta
     8461 tcatggataa tgataagtgg gttaaacttt aatagtgtta tgatatgaca gtaatcttag
     8521 gatttttatt tctatttttg atgagttggt gggttatttc aatcgttaat agccagaaac
     8581 cacgcaaaaa tgaaacattt attggctatg tccaaagata cgatattgat gggaatggct
     8641 atcaatcatt accgtgtcct cagaactgag aggagttctt gaaatcgtaa aaccactttg
     8701 aacaagagtt acttcaaccc attggctccg acggattaag ttgctttcgt gaataccaaa
     8761 atcagccgca atttcttcat aagtgcggta ttctcgcaca tattgaagag tggccataag
     8821 aaggtcttct aggcttaatt taggttttcg tccacctttt gcgtgtttaa gttgataagc
     8881 tgtttttaat acagctaaca tctcttcaaa agtcgtgcgc tgaacaccaa caagacgctt
     8941 aaatcgtgca tcagttagtt gtttacttgc ttcataattc atagaactat tataccatgt
     9001 tttgtttcgc aggaaatcta atgctaaaat cttatcagtg agaagatttc ttaaagttta
     9061 ttctttgtag tttcatctgt attagtacat aatttaactt agagaaaata ggaggactaa
     9121 tcgaatggaa ttaaaagatt ttacagaaaa agaacaggaa atgattaaga aaaggcttac
     9181 aatgtctaat attagtgaca aagaaactac tgagaagatt ctggcgctag taccacaaga
     9241 cttgattaag cgaatcccgt tttttgtcag aaaacatgct acaacacgta cgattaaacg
     9301 catttcaatt gaacaccctg aactctacgc tgtagctcaa acaagtggtg agattccaga
     9361 aaaagaacgc gaagaattgc gtcagattat cacaactatc tttgaacaaa agatgaataa
     9421 gcatagtatt aagtagagaa tgaaaaaata ttttattggc ggtttgggaa acaatgccta
     9481 tcatagcaag gattttcttc aagaactaga ttcgcaggtc tattttctaa atccatatga
     9541 aaagcatctt cgagatgaaa cagaattgaa atcatggttt aaaaatgaga ttgtagagga
     9601 agaatctatc tgtctgatag gtcattctat tggaggcgat ttagctcgtt atttcgcatc
     9661 ggaatttgaa gaagtaaaga aactgattct tttggatggt ggctatctag atttagataa
     9721 gattttacct ttggatacag agttagagga aactaaaaat tatatcaaat ctcaaattat
     9781 ttcggactta gatgttctta cttctaaaga aaaatctgaa gcaaagcatt ggtcagaaaa
     9841 tatggagaaa gctgtaagac agtcctatca ctggaatgtt gagtataata gatatgagtt
     9901 ggctataaat tatgaaaata tagaagcgat actccgccta cggagaaaaa tacaagcttt
     9961 taagagagaa gtgggagata ccttgtttat tagtcctcgc tatcctaatg aagctacatg
    10021 gagagaggaa gccctaaaag aattgccaga ctattttgat actatttttt tagaaaactt
    10081 tggccatgag ctttatactc aagcacctaa agaaatcgct agtctaatga atgagtggct
    10141 cgcttatttt ctatgagcta cttgtattgc tggaaatggt ataagatgaa aatctacaag
    10201 tcactaattt tcatataacc aaaaaacgaa caggctcttt ttctgttatt cagaagagaa
    10261 cttgttcgtt tttatcttga ttttcttaaa aagctctata tacataggga ttttctggtt
    10321 tatcgacttt ggtaaagctg tcgatttcca aggttggaag gttttgtttg agttctttat
    10381 ggtagtgatt gaccagtttt cctttggctg aaatccaagt gttgttttca tactgccggg
    10441 tattgccctt ggtcagcaat ccatagacac cagaatctgc gatgcagtga ataatgccga
    10501 agcggaacag aaattggcta ttggcatgac tggggtcatt atagacaaag cctgtgaact
    10561 ggattgtctt accctcaaat tcatctggat agtcgtagat agcctccatg acctccatat
    10621 agttttcatt agtgatctga atactatctt gggataagta tttatccgcc gccgttcgca
    10681 tttccttttc ataggctgat tttgaaaaat aagaactggt atctggtttc aaatattggc
    10741 ttgtcgtccc ttcgcttgtc tgaatggcta gatccgttcc ttccgatagg gggaaatgat
    10801 aacctttagc agaaacggtc tgagaatcca agctaacagt tgggaaagtt aagccgatga
    10861 caagtggaat agccagaaga gaaatactcg tcatcttggc taatcggctg ttcagatgac
    10921 tgtgggtttt gacttgcttc atccagatat acaattgaac gatagccaag ataaaagaaa
    10981 gcaccatgga gatataggcc agataggaat agtgcatgtt gatgtactgg tttagtttgc
    11041 ccgacagatg gaggtaaata gtcagttcaa aatagccagc taaaactaaa aatcgaatca
    11101 taggatgact ccaatcaaga gagaatagac taagacgaca agagttacaa ttgttatgaa
    11161 gtgactgata aatcgtgctt tcaagtaatt tttcatcatg agaatatttt tgatatccag
    11221 cattggacca atgacgagaa aggccagaac tggtgccaaa ccgaaactcg agagaagaga
    11281 agcacctata aaggcatccg cctcactaca gagcgaaaga agaaaggcta aaatcatcaa
    11341 gagcaggatg gcaaaaagag gggtcgcact gatagaggtc agaatccgag tcggaacgta
    11401 gacctgtatt atagaagcaa agaggcagcc aaataccaaa taacgccccg tatcaaaaaa
    11461 ttcatcaatg gcctgcacaa agacttgaaa aacttttttt gcagaactca agtaagaaaa
    11521 atcatgctca tgacaagcca gacgattttc tttctgaatc ggttcttgcc agaaaaatcc
    11581 tagaaaaatt cctagtatta cagccacaag aatggaaccc agagctcgta atagggcgac
    11641 atggaaggag ttgccaaagg cagaataggt cgcaaaaaga acaatgggat tgataacagg
    11701 tgctgtcaca agaaaaggaa cggccgtgta acttggaacc tttttttcca gaaaacgatt
    11761 gatgatgggg acgattccac attcacaaga agggaaaaac ataccgacga aggtcccaaa
    11821 aaagattctc ccccaacgat ttcgagggag aaaatgataa accttgtcag gtgtgatata
    11881 aacttcaatc agccctgaga caatgcttcc tatcagaacg aagggaaggg cttcaatgat
    11941 aatggagaga aaaatggctc cagtttgaag tacactagat gggagttgtt gaaaagcagt
    12001 catctagttt ttcttttctg ctttggcttt tttctctttg tcatcaggga aagtaacttg
    12061 tttcaaatca gggagtttag caaactcttc aaacatcttg tccaagtcgt ctcgttttgt
    12121 aaattttaca gccataggtg tacctcgatt ctaaattcta cctctattat actattattt
    12181 tgcggaaaag ggagtttttt aatttgtttt cgatagttga tactcttcga aaatctcttc
    12241 aaaccacgtc agcttcgcct taccgtagta cggttactga cttcgtcagt tctatccaca
    12301 acctcaaaac agtattttga gcagcctacg gctagcttcc tagtttgctt tttgattttt
    12361 attgagtatg aaaaggattg gagaaagcct tccagcttgt ggtataatag atttatggat
    12421 aaaaaatatg aaaaaatctc tcaggatttg ggagtgacgt taaagcaaat tgataccgtt
    12481 ctaagtttga cagctgaagg ggcgactatt ccctttatcg cgcgttatcg caaggacatg
    12541 actggtagtc tggatgaggt ggcgattaag gctattattg atttggataa aagtctgaca
    12601 aatctcaatg accgtaagga agctgtctta gctaagattc aagaacaagg taagttgacc
    12661 aaggaattgg aagaagctat cttagttgcc gaaaaattag cagacgttga agaactctat
    12721 cttccttata aggaaaagcg tcgtaccaag gcaaccattg cccgtgaagc tggactcttt
    12781 cctcttgctc gcttgatttt gcagaatata gttgacttag agaaagaagc tgaaaagttc
    12841 gtctgtgaag gatttgcgac tggcaaggaa gccttgaccg gtgcagttga tattttggtc
    12901 gaagccttat cggaagatgt gaccttgcgt tctatgactt atcaggaagt gctgagacac
    12961 tctaaactca cttctcaagc caaggatgaa agtcttgatg aaaagcaggt ttttcagatt
    13021 tattatgatt tttcagagac agttggaact atgcaaggct atcgtacctt ggctctcaat
    13081 cgtggggaga aacttggtgt cttgaagatc ggttttgaac atgcgacgga ccgtattctt
    13141 gccttctttg ctactcgttt caaggtgaaa aatgcttata ttgatgaagt tgttcagcaa
    13201 tccgttaaga aaaaggtctt gcctgctatt gagcgtcgta ttcggacaga attaactgag
    13261 aaagctgaag agggagctat ccaacttttt tctgacaatc tgcgcaatct cctcttggtt
    13321 gctccactga aagggcgcgt ggttcttggg tttgacccag cctttcgtac aggtgccaag
    13381 ctagctgtcg tggatgcaac aggaaaaatg ctgacaactc aggttattta tcctgttaaa
    13441 ccagcatcag ctcgtcaaat cgaagaagcc aagaaagatt tagcagattt aattggtcaa
    13501 tacggtgtag agattattgc cattggaaat ggaacggcca gtcgtgaaag tgaagctttt
    13561 gtagcggaag ttctgaaaga tttccctgaa gtcagctatg ttatcgttaa tgaaagtggt
    13621 gcttctgtct attctgccag cgaacttgct cgtcaggagt ttccagactt gaccgttgaa
    13681 aaacgatctg ccatttctat cgcccgtcgt ttgcaagatc ctcttgcgga attggtcaaa
    13741 atcgatccta agtcaattgg tgtcggtcaa taccaacacg atgtcagtca gaagaaacta
    13801 tctgagagtc tggactttgt tgtcgataca gtggttaacc aagttggtgt caatgtcaat
    13861 acagctagcc cagctcttct ttcacacgta gctggactca ataaaaccat ttctgaaaat
    13921 atcgtcaaat accgtgagga agagggaaaa atcacttcac gctcccaaat caagaaagtt
    13981 cctcgtttgg gggccaaggc ttttgaacag gctgctggtt tcctccgtat ccctgaaagt
    14041 agcaatatcc ttgataatac aggagttcac ccagaaaatt acgctgccgt taaggaactc
    14101 ttcaaatgct tggacattaa ggatctgaat gaagaggccc aaagtaaact caagtcgctt
    14161 tcagtcaagg aaatggcgca agagttagac cttggtccag aaactcttaa agatatcatt
    14221 gcagatcttc tcaaaccagg tcgagatttc cgtgattcct ttgatgctcc agttctccgt
    14281 caagatgtcc tagatatcaa ggacttagtg gttggacaaa agctagaagg agtggtgcgt
    14341 aatgttgttg atttcggtgc ttttgttgat atcggaattc atgaggacgg tctcatccat
    14401 atttcccata tgagtcgcaa gtttatcaaa caccctagtc aagtggtggc agttggagat
    14461 ttggtgagcg tttgggttaa gaaaattgat actgaacgtg aaaaagttaa tctgtctctc
    14521 ctagctccaa atgaaactga ctgattacgt caagcaggtt tcactagaag acttcggcag
    14581 accttttatc catcatgtcc agtggaatag gcgtctacgt tcgacaggtg ggcgattttt
    14641 ccccaaagat gggcatttgg attttaatcc caaggttggg gttggacgtt tttaggaaaa
    14701 ttgtccgaca tgaactctgt cattatcacc tctattttca agggaaaggc tatcaacaca
    14761 aggatcggga ttttaaggaa ctgttgaaag cagtggatgg attacggttt gtaccatcct
    14821 tgcccaatag taactccaag ccaatcaagc tctatcgttg ccaatcctgc cagcaaactt
    14881 atcagcgtaa gcgtaggatt gatacccaac gctatcgctg tggactttgt cgaggaaagt
    14941 tgctattagt aaatcagcct gaggactgat gaaagccgag cctatccgtg atatactagt
    15001 ctaacctagc agaaagagga aatatcatga acagtaagtt ttataaaatg agacgaaatc
    15061 gcatgatttc aggagtttta gcaggtctgt cagacaagtg gaactttgat gttacccttg
    15121 tacgttttct gtttgccatt tttacagtcg caaactttgg acttggggtg attatctata
    15181 tcatcctagc ttctatcatg ccaaccaagg aagaaatcga agcagaaatg tacggaacag
    15241 gcccacgtaa acgcaaagaa gctcaagcca ttgacgataa tgaaggctgg ttttggtgag
    15301 gttttacaaa agatttggaa tacgcaaaaa gctttaaatc aatagtctaa actgcttgat
    15361 ttaaagcttt tttactttta tactcaataa aaatcaaagt gcaaactagg aagccagccg
    15421 caggctgtaa tttagtcggc aatgtgaagt tgatatagtt tgcactttga tttttgaata
    15481 gtattaataa tatttcgcca gcttcatcca caaactatag tggattggaa ctagaatagg
    15541 acacctatac ttctaaaaaa ttgttagaaa tcgatttgac tgtcctgatc gatttgtcct
    15601 gttcttattt cattttacta taaaagcagt aatttaaacg cagtttttta atctattttt
    15661 gctgttgaga caatacattt ttggaaaaat ggtggatgct gataacaact gataaccagc
    15721 aaataacata agcagtgccg gtaaattctg taccgataaa gtgtgtgtgt tcaactaaga
    15781 gctgaccgat gaatggagag agcagataga gaagtccgat gagtatatgt tctttgtgtt
    15841 tcattaaagc gcaccttctt tccttatagg ttgaacgact tgcaagtgtt aaattgaatg
    15901 gtatgttatc taggtaagct tactcaaatg atagttcccg cttcctcctt ttcaaagtaa
    15961 gccttttcac acttatattt tacaacttta aaaataggaa atcaaatcat tttctaagta
    16021 attttgctta tatttcaccc aatcttacaa aaatgtaaga ttaaagtctc ttttgtaagg
    16081 ttaggttatg gcaagcggtc tttttttgat gtaaactaga ctcataaaga acaaaggagt
    16141 aacatcatga aacaaatctt acataagaaa actagaaaac caagtcacaa agaaattgag
    16201 cgcgttcagc tgggatgcac tatgatgcaa gctacatttc atttgatggg atattaagaa
    16261 aggagattct catgacactt ttagatgtaa aacacgttca aaaaatttat aaaacacgtt
    16321 ttcagggcaa ccaagtagaa gccctcaagg atattcactt taccgtcgaa aagggtgact
    16381 acgttgccat catgggtgag tctggttctg gtaaatcaac tcttctcaat attctagcta
    16441 tgctggataa accaagtcgt ggtcaggttt acttgaatgg aactgacacc gcaaccatta
    16501 aaaattcaca ggcctctagc ttccgtcgtg aaaagttggg ctttgtcttc caagacttta
    16561 acttgctaga tactctatct gtaaaggaca atatcttgct tccgcttgtc ttgtcaagaa
    16621 gacctattac agagatgatg aagaaattgg tggtgacagc tgagaatctg ggaataaacc
    16681 aattacaaga gaagtaccct tacgagattt ctggtggtca gaaacagcgt gtagcagtag
    16741 cccgcgccat catcacagaa cctgaaattc tccttgcgga cgaaccgaca ggagctcttg
    16801 actccaagtc atctgcagcc ttacttgatg tctttgatga aatcaatgag cgcggccaaa
    16861 ccattctcat ggtaacccac tcaacagcag cagctagcag agctaaacgt gtactcttta
    16921 tcaaagacgg cattctttac aatcaaatct accgtggaga gaagacagaa cgtcagatgt
    16981 tccaagaaat ctctgatacc ttgactgtca tggcaagcga ggtgaattag tatgtttcga
    17041 ttaaccaata agttagcggt atcgaacttg attaaaaacc gcaaactcta ctatcctttt
    17101 gcgctggctg ttctcttggc agtcaccatc acctatctct tttactccct aaccttcaat
    17161 ccaaagattg cggaaatccg tggaggaacc accattcaag caacacttgg atttggtatg
    17221 tttgtcgtta cccttgcgtc agccattatc gtcctctatg ccaatagttt tgtcatgaaa
    17281 aaccgttcca aggaactggg tatatatggc atgttaggct tggagaagcg ccatctaatc
    17341 agtatgacct ttaaggagtt agtgctattt gggattctaa ctgttggagc aggaatcggt
    17401 attggagcct tgtttgacaa gttgattttc gctttcctac tcaaactgat gaaattgaag
    17461 gtggaactgg tcgctacctt ccagatgaaa gttgtcatta cagtacttct tgtcttcggt
    17521 ttgattttcc taggcctcat gttcctgaat gcccttcgaa tcgcccgtat gaatgccctc
    17581 cagctctcac gtgagaaagc tagcggagag aaaagaggtc gtttcctacc tctccaaact
    17641 attcttggtt ccataagttt agggattggc tattatcttg cccttacggt aaccgatcct
    17701 cttacagccc taacaacttt cttcctagct gttttgctgg ttatctttgg tacttatcta
    17761 ttgtttaatg cagggattac agtcttccta caaatcttaa agaaaaacaa gaaatactat
    17821 taccaaccta ataacctcat atctgtttcc aacttgattt tccgtatgaa gaaaaatgcg
    17881 gttggactag caaccatcgc tattttgtca acaatggttt tggtaaccat gtcagcagcg
    17941 acaagcattt tcaattccgc agaaagcttt aaaaaagttc taaatcctca tgattttggg
    18001 gtttcagggc aaaatgttga aaaagaagat ttggacaaac tcttgagcca gtttgcaagt
    18061 gacaaaggtt atagtgtcaa agagaaagaa gtacttcgtt acagtaactt tggtattgca
    18121 aatcaagaag gaaccaagtt aactattttt gaaaaaggac aaaaccgtgt ccaacccaaa
    18181 acagttttca tggtatttga ccaaaaagat tatgaaaata tgactggtca aaaactgtct
    18241 ctatcaggaa atgaggtcgg tctctttgcc aaaaatgacg gactgaaagg acagaaagct
    18301 ctaactctaa atgatcatca attttctgtc aaagaagaat ttaataaaga tttcattgtc
    18361 aaccatgttc caaataagtt taatatcttg actactgatt acaattacct tgttgttcct
    18421 gatttacaag cctttttgga tcaattccca gattcggcta tctataatca gttttacggt
    18481 ggtatgaatg tagatgccag cgaagaagaa caactcaagg tcgctgagga gtatgaaaac
    18541 tacctcaatc aatttaatgc tcaattagac acagaaggta gctatgttta tggtagcaat
    18601 ctagcagatg ctagttctca gatgagtgcc ttctttggtg gtgtcttctt tatcggtatt
    18661 ttcctatcca ttatctttat ggtcggaact gttctggtca tctactacaa acaaatttct
    18721 gaaggctacg aagaccgtga acgctttatt atcttgcaga aagtcggttt ggaccaaaag
    18781 caaatcaagc aaaccatcca caaacaggtt ttaactgttt tcttccttcc tttgctcttt
    18841 gccttcatac atctcgcctt tgcctaccat atgcttagcc tgattttaaa agtgattggt
    18901 gtactggata cgactatgat gttgattgtg accttgtcta tctgcgctat cttcctcatc
    18961 gcctatgtgc tgattttcat gattacttca agaagttatc gcaagattgt gcaaatgtaa
    19021 aaaagatacc tcgacttcaa aatcgaggta tttcttgtat tttaaatgct gaaaagttgt
    19081 tcgagcagga aggtaactcc catggtcaag agaccaatag caaggttccg aatcatagct
    19141 gttttggttg gggcttttcc aagtctagca cttgtgtaac cagtgagaag aagggccaca
    19201 ccgacaataa ggacggtagc agggatgcgg taatcacttg gaaaaatggt cactgagagc
    19261 attggaggca aacttctaag gaaaaaggca acgaagctag aaatggcagc gtgccaagga
    19321 ttggtaaatt cttcatactc aatcccatat ttttcctcta ccagagcctt gagtggattt
    19381 ttaagaaaga tcttattagt caagagttgg gcagaagttt tgaattctcc attttggata
    19441 taagaagcat agagggattt tttggctagt tccctatctt ggtctagcaa gagtttttct
    19501 cgcgaaacgg cagcttcctc ggtatctttt ggagttgaaa cggatacata ttctccacca
    19561 gccattgaaa aggcaccagc taagatagcc gcaaaacctg ataaaaagat aatccagata
    19621 ttgctcgtgg cactggcaac tccgataacc acaccagcaa tggaaataat tccatcgtta
    19681 gcatcaagaa cacccgcacg caggatattt aaacgacctg caaaatttga atcaatttcg
    19741 tgatttgttt ctgacgctaa atttcaagtt caagttagcc atccagaagt cttctctgag
    19801 tgacttgtag tccaagcatt ttttaggata gttgtt
//
LOCUS       DAVLVZ010000033        18750 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000033 DAVLVZ010000000
VERSION     DAVLVZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.033"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(113..766)
                     /gene="phoU"
                     /locus_tag="VYN77_001223"
     CDS             complement(113..766)
                     /gene="phoU"
                     /locus_tag="VYN77_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1558683.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(778..1536)
                     /gene="pstB"
                     /locus_tag="VYN77_001224"
     CDS             complement(778..1536)
                     /gene="pstB"
                     /locus_tag="VYN77_001224"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1558684.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLNPQHKETEDYITGKFG"
     gene            complement(1549..2352)
                     /gene="pstB"
                     /locus_tag="VYN77_001225"
     CDS             complement(1549..2352)
                     /gene="pstB"
                     /locus_tag="VYN77_001225"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1558685.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQALDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(2363..3247)
                     /gene="pstA"
                     /locus_tag="VYN77_001226"
     CDS             complement(2363..3247)
                     /gene="pstA"
                     /locus_tag="VYN77_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1558686.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(3237..4154)
                     /gene="pstC"
                     /locus_tag="VYN77_001227"
     CDS             complement(3237..4154)
                     /gene="pstC"
                     /locus_tag="VYN77_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1558687.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(4154..5032)
                     /gene="pstS"
                     /locus_tag="VYN77_001228"
     CDS             complement(4154..5032)
                     /gene="pstS"
                     /locus_tag="VYN77_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1558688.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(5606..6910)
                     /locus_tag="VYN77_001229"
     CDS             complement(5606..6910)
                     /locus_tag="VYN77_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1558689.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(6911..7684)
                     /locus_tag="VYN77_001230"
     CDS             complement(6911..7684)
                     /locus_tag="VYN77_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1558690.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNGRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGHLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(7674..7952)
                     /locus_tag="VYN77_001231"
     CDS             complement(7674..7952)
                     /locus_tag="VYN77_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1558691.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(7952..8353)
                     /locus_tag="VYN77_001232"
     CDS             complement(7952..8353)
                     /locus_tag="VYN77_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1558692.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(8563..9180)
                     /locus_tag="VYN77_001233"
     CDS             complement(8563..9180)
                     /locus_tag="VYN77_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1558693.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(9170..9943)
                     /locus_tag="VYN77_001234"
     CDS             complement(9170..9943)
                     /locus_tag="VYN77_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1558694.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLLIAPTHVVSSLAEWDFDEN"
     gene            complement(9954..10691)
                     /locus_tag="VYN77_001235"
     CDS             complement(9954..10691)
                     /locus_tag="VYN77_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1558695.1"
                     /translation="MCLTYQMKIKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(10696..11826)
                     /locus_tag="VYN77_001236"
     CDS             complement(10696..11826)
                     /locus_tag="VYN77_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1558696.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(11910..12299)
                     /locus_tag="VYN77_001237"
     CDS             complement(11910..12299)
                     /locus_tag="VYN77_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1558697.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(12315..12698)
                     /locus_tag="VYN77_001238"
     CDS             complement(12315..12698)
                     /locus_tag="VYN77_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1558698.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(12699..13487)
                     /locus_tag="VYN77_001239"
     CDS             complement(12699..13487)
                     /locus_tag="VYN77_001239"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1558699.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(13480..14415)
                     /locus_tag="VYN77_001240"
     CDS             complement(13480..14415)
                     /locus_tag="VYN77_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1558700.1"
                     /translation="MSVLVKEVIEKLRLDIVYGELELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(14545..14721)
                     /locus_tag="VYN77_001241"
     CDS             complement(14545..14721)
                     /locus_tag="VYN77_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1558701.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(14861..15568)
                     /locus_tag="VYN77_001242"
     CDS             complement(14861..15568)
                     /locus_tag="VYN77_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1558702.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            15719..16747
                     /gene="queA"
                     /locus_tag="VYN77_001243"
     CDS             15719..16747
                     /gene="queA"
                     /locus_tag="VYN77_001243"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1558703.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGSHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQRIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            complement(17296..18423)
                     /locus_tag="VYN77_001244"
     CDS             complement(17296..18423)
                     /locus_tag="VYN77_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1558704.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGSIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEKVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFTTKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKGQEVGEIG
                     KITLTTIYAVDERDGTIEETTSRQITKEMVKRRIRRGTREPEKVVVPEQSSIPSYPVS
                     VTSNQGTDVAVEPAKAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGS
                     MKVNQWFQVGGKWYYVNTSGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT         5558 a         4312 c         3425 g         5455 t
ORIGIN      
        1 ccaagttact tgtcaagtgt tttttaaatt tttatctcaa aaatattttt tcgttcaaaa
       61 aaaggagcca tcagttgatt tcaagctcct ttttatacag aattaaacta ttttatagtt
      121 cgacaatctt acctgtttca aagtagacaa cccattcaca gatattttta gcatagtcac
      181 cgatacgctc caagtaggaa ataacttgga aataatcacg acccgtaaca atggcttctg
      241 gatttttctt aatctcttca gtcgcaaggt cacggatagt ttcaaaatag tggttaattt
      301 gctcatccat ggaggccacc cggtatgcgt cgtcaacaga accattaaga taaagatcaa
      361 gtgctgcttc aacaacgctt ttaacttcac gtcccatttt tttaatttct tcctctacag
      421 ctggaatgcg ctcttccccc ttcatacgga tggttgcctg agcaatggct acagcgtgat
      481 cccccatacg ctccacatct gatacagcct taaggacagt caagactgta cgcaaatctt
      541 gagagactgg ttgttggagt gcgatcattt caaatgattt cttttccagt ttcacttcgt
      601 attcatttac ttctgcatca tcttcgatga cctcttttgc caggtcacgg tcatgcgtga
      661 caaaagcacg taccgtacga ttgatttgtg agagcacttc ttgtcccata gcgtagaact
      721 ggttatgtaa tttctctaaa tcttcttcaa attgagatcg taacatcttt catctcctta
      781 tccaaatttt cctgtaatat agtcttccgt ttccttgtgt tggggattaa ggaacatctg
      841 cttggtatca ttaaattcaa tcaaatctcc atctaggaaa aatcctgtct tatcagagat
      901 acgtgaagct tgctgcatgg aacgggttac cagaagcatg gtgtacttgt cttttagacc
      961 atacaaggtt tcctcaattt taccagctga aatcggatcc aaagccgaag ttggctcatc
     1021 caagaggatg attttaggac tagttgccaa gacacgggcc acgcagacac gctgctgttg
     1081 accacctgac aatccaatag ctgaatcata tagacgatcc ttgacctcat cccagataga
     1141 ggcaccttgc aaggcttttt ctacggcttc atccagaacc tgcttatcct taattccatt
     1201 gatacgaagc ccgtagacaa cattctcata gatagtcata gggaaaggat taggttgttg
     1261 gaaaaccatt ccgatttcct tacgtaattc aaccgtatct gtacgcggac tgtagatgtt
     1321 gtgaccattg tacaccacgg atccagttgt ggtcacctct ggattgagat ctcccatgcg
     1381 gttgagagac ttgaggaggg ttgacttccc tgatccagat ggaccaatca aggctgtaat
     1441 ttccttaggt tggaaagata gggaaacact attcaaagcc ttctttttat tataataaac
     1501 ggacaggtct gatacctgta aaatcgcatc tgtcatacgg tttcctttct aaccaaagtg
     1561 accagataca tagtcattgg tggactgtag cttggcattt tggaaaatag ttgcagtctt
     1621 gtcatactca atcaaatcac ccaagtaaaa gaagcctgta tagtcacttg cacgagcagc
     1681 ctgctgcata ttatgcgtta caatgatgat ggtaaagttt ttcttgagct caaacatggt
     1741 ctcttctagt tgcatggtcg caatcggatc caaggctgag gctggctcat ccattaagag
     1801 gatatctggc ttaacagaga tggcacgagc gatacagaga cgttgttgct gaccacctga
     1861 taaggtcaag gctgacttgt ggagatcgtc tttaacctga tcccaaaggg cagcctgacg
     1921 aagggaggtt tctacgattt catctagggc ttgcttatcc ttaactccag cacgttcatg
     1981 cgcaaaggta atattacggt aaattgactt agcaaatgga ttggggcgtt gaaaaaccat
     2041 tccaatgtgt ttacgcattt cataaacgtt gatttctgga cggttgacat caattccacg
     2101 atagagaatc tgcccagtta ctttagcaat atcaatggta tcattcatgc gattgagact
     2161 gcgtaagtag gtagatttcc ccgatcccga cggaccaatc aaagctgtaa ttttatttct
     2221 ttcaaattgc atatcaatcc ccttaatgga ttcattttta ccatagtaaa catggacatc
     2281 cttagtagaa agggctactt tttcttcagg aaaggtaagg atatgcttct catcccagtt
     2341 atatgttgac atggcttctc ctttaggcag cggttaattt cttgtgtaga tagcttccga
     2401 acttacgagc tccaaagtta aaaatcagga taaagatcag gagcacagcg gcagaacctg
     2461 ctgatacaat ggttccatct ggaatagtgc cttcactatt gactttccag atatggacag
     2521 ccaaggtttc tgcttgacgg aagatagaga tggggctagt cacactgagg atattccagt
     2581 tagaccagtc aagagctggc gccgattgcc ctgctgtata gatcagagct gcagcttcgc
     2641 caaagatacg accagatgcc aaaacgacac ccgttacaat acctggaagc gcttctggaa
     2701 taacaacatg aaccactgtc tcccagcgag aaatcccaag agccagacca gcctcacgtt
     2761 gggtatggtg aacgtgtttc aaactatcct ctacattacg cgtcatctga ggcaagttaa
     2821 agactgtcaa ggccaaggca cctgaaatga ttgaaaatcc atactcaaac tggactacaa
     2881 agatcaagta accaaagaga cccaccacca ctgatggtaa agaggacaag atttcaatac
     2941 aagtccgcac aaagttggta acaggacctt ttttagcata ttcagccaag taaatcccag
     3001 ctcccataga aagaggtaca gaaataatca aggtaatgac caataggaaa aaggaattgt
     3061 aaagctgaat gccaatccca ccacctgctt gaaaagcaga agaccttcca gtcaagaaag
     3121 accaagagat atggggcaag ccccgaacca agatatagag aatcaaggaa gccaagattg
     3181 tcacaatgat gctagcaatc gtatagagga cagctgttgc aagtttatct aatttcttag
     3241 cgcgcataat ttttctttcc tctttctttc gtaatcaatt taatcacact gttaaaaact
     3301 aaactcatca agagcagtac caaggccagt gaccagagaa cattattatt tacagttccc
     3361 atgacagtgt tcccaattcc catagttaat atagaagtta aagttgcagc tggtgtggtc
     3421 aaggaagttg ggataacagc tgagtttccg acaaccatct ggatagctag agcctcacca
     3481 aaggcacgcg ccatcccaaa gaccactgca gtgaaaatac cagaacgggc cgccttcaag
     3541 atcacacgcc agatagtctg ccagcgagtg gctcccatag cgaaactggc ttcacgataa
     3601 taacgaggaa ccgcacgcaa gctatccgtt gtcataaagg ttacggtcgg caaaatcatg
     3661 acaaagagga cggaaatccc tgacaaaatc ccaaaaccag tcccaccaaa gacactgcga
     3721 acaaagggaa cgacgacttg caagccaata aatccgtaca ctactgaagg aatcccaacc
     3781 aggagttcaa tagctggttg caaaatcttc gccccttttg gtgatacttc ggtcataaaa
     3841 actgctgcac caatagcaaa gggtgttgcg ataagggctg agagaatggt aacgataaag
     3901 gaacccaaaa tcataggaag ggcaccaaat tctttactag aaggattcca agttcctccc
     3961 aaaagaaagt caaagatatt cacaccattg acaaagaagg tcgacaagcc tttttgcgct
     4021 acgaaaacca aaatcatggc cacaaggatg actatcaaag aaagacaggc aaaggtcaaa
     4081 ccttttccta atttctccag acgagaattc tttgatggaa gcaacatttt cttagctaat
     4141 tcttcttgat tcattattgt ctcccttcca acactgtcac agttccggca gcatcttttt
     4201 caaccttcat ttccttaatc ggaatatact tcaatccttt gacaatccct tcttgggtct
     4261 catccgagag aacaaaattg agaaattctg cagccaactc attgggctgc cccaatgtat
     4321 acatatgctc ataagaccac aagggccaat tattgctact tatattttct ggacttaagt
     4381 catagccatt caacttcatg cttttgaccg aatcatctat ataggtaaga gataaataag
     4441 agatagctcc tggacttttt gatacgattg attttaccgc tccatttgaa tcctgctcct
     4501 gactttgcat ggcagactga ccttccataa tgacagtatc aaaggtagca cgagagccag
     4561 agccggctgc ccgattgata acagagatgg gtaagtcctt accaccaacc tctttccaat
     4621 tggttacctc acctatgaag atttgacgaa gttgctctgt cgttaggtta tcaacatcaa
     4681 cctccttatt gacaatcaga gccaagccag ctaccgcgac cttgtggtca acaagagcag
     4741 aagcatcaat tccgtctttt tcctcagcaa atacatctga gtttcctata tcaactgccc
     4801 cagactgaac ctgggacaag cctgtaccag aacctccccc ttggacattg accgtttttc
     4861 caacatggat cgtgccaaat tcatctgccg ctacttcaac caagggttgc aaggcagttg
     4921 agccaacagc cgttatggat tctccacgat caatccagct agcacagcct actaaacaag
     4981 ccgtcagcca aaaagcgata agagacagag caagcttttt tctttttttc actgtttttc
     5041 tcctcgaaaa taattatgaa tactgtgaat tttttaagta gttctttatg agttgacgca
     5101 tgaattctta ccaaatttct gcgcaattga ttatttatat aatataggct atattactct
     5161 ttcctaacct ccttttttca tatgtggata aaatctcttg tctatccctt cccccattgt
     5221 cacccattat agtcatttcg tgtctctttt tccccttttt aatgcaaggg aaattactct
     5281 tcttagatga taatccaaaa gctagaaagg tatctcaaac ctctctactc tcccagacta
     5341 gtttacaact aaaaggaaaa gattctattt tatgagaaat ctagtttaca agcggtaaga
     5401 acgctaataa ctaaacttct tgtactcttt gaaaatctct tcaaagcagt gttttgagct
     5461 atctatggct agcttcctag tttgctcttt gattttcatc gagtagtaaa actacatgtg
     5521 atggcaatca agatatcaag aatcatccta ctaaaaaaat ccatactttc actataacat
     5581 agaataagat atttgactag cattttcatt tgaatctgag gccttttgga aaataatttt
     5641 tcaaaacatt tccagtaacc tttgcaaagc ccaagccatt gcctttaacc aaaacttggt
     5701 accaaccatt tggcagactt tctgccagct gaacggtttc tccagccgca tacttgacaa
     5761 acgcttcttg gccaatttca accgactgtt cgacctgact cggtttcaag gctaaaccaa
     5821 gagcgaaact gggctcaaag cgtttcttct taaaagtacc cagatgcagt ccattgcgag
     5881 caatcttgag cttccctaaa tctggcaaaa gttctggcaa gagataaagc tggtctccaa
     5941 aaatctgcaa gatacccggt agattgacct tcaaatggtt ttgggcaaat tcctgccaca
     6001 aggcaacttg ttcacggctg aggttactct tacttgcctt aaatttagga gctggattgt
     6061 tacccttaaa ctgtagatgg gcaacaaact gaccctctcc cttaaactga tgagggtaca
     6121 tccgagccgt ttctggcagg tcaataccag ctaccattcc attgatatgc tctactggca
     6181 acaagtcaaa atcatactct tccagcaacc aattgacaat ctcttcgttt tcctcgggtg
     6241 cccaggtaca ggtcgaataa accagacgac caccttcagc taacatggtc actgcatcct
     6301 ccagaatttc tctttgcaag ctagcacatt gactcggata atctaagctc caatagtcca
     6361 tagcatcagg ttgcttacga aacattcctt caccagagca aggggcatca agaacgatta
     6421 agtcaaaata gcctttaaag accttgacca agcggtcggc agattcattg gtcaccacga
     6481 catttgtcgc tccaaaacgc tccatgtttt caaccaaaat cttagcccgt ttgcttgaaa
     6541 tttcattgga aacaagtagc ccctcccctg ctagataggc tgccagttga gttgatttgc
     6601 cccccggtgc agcagccaag tccaagacct tcataccagg actgggttgg gctacttgag
     6661 ccaccatttg agcagcaggt tcttgcgaat aaactaaacc tgtagcatgc tcaggcgatt
     6721 tccctgaaac cttcccatag tggccccaag gggtttgagt aatggcatca gaaaaggaaa
     6781 gttgctcttc ttttaaggga ttgacccgaa aggccgaaac cgcttcctcc tcaaaagagg
     6841 caagaaaatc tcttgcctca tctcctagta tttctttata tttttcaaca aatccttctg
     6901 gaaatttcat ttaagttctt ttcctttcgt aaatatagga ctgaatttcc tcctgcatct
     6961 caagaggcac catcatgacc ggctgtctgg tttgaaaatc aggagcttca ccaaaaaggg
     7021 tcacaacccg atagcccaga ctttccccta aaatactagc tgcggcataa tcccatggtt
     7081 gcagataggt gagataggtc aacaaatgcc ctgacaaaat cttggcaaaa ctaatggccg
     7141 cacttccata gacacgaaca ccaagaaccg ctcggctcaa atcagccagc ccccattcat
     7201 tggtttccag cataccacta ttccctgcaa tgagaaaatc tccaagtggt ttagttttaa
     7261 aaggagctag gggcctacca tttagacaaa ctggaaattc cccaccaccg tggtaacaat
     7321 cccctttgac cacatcataa atcagaccaa actgtccctg accattttca aaataagcca
     7381 tcataacagc aaaatcttcc tgctgggcta caaaattatt ggtaccatca atgggatcaa
     7441 tgacccaaac cttgccctct tgaaccgagg ctcgcagaca accttcttca gcacaaatct
     7501 tatcctcagg ataacgggac aaaatctcac caaccaagag ttcctgaact tctttgtcca
     7561 gtctggtcac caaatctgtt ggagaggact tggtttcaac acgcaagtct tcctgcatat
     7621 ggtcaagaat gtactggcct gctttcttaa caagctcttt agcaaattca aatttacttt
     7681 ccaagagaaa tctttccttc ccctttttct ttggcagcct gaactgctcg gtaaagtgaa
     7741 tagccactaa ccgtttcaaa ttctcgtccc aaacgtttct cttcgctctt gcttggcacg
     7801 accgctttga atcccttata ggagtccagt aactttttag cctctacttt gccttcatag
     7861 gcagcttcaa catcattaaa aaaagaaagc actgaagcaa gttcttcagt gctccacgac
     7921 aaatctagtg ggtaactata ctgtttgttc attaactaat accagctctc attcttgctt
     7981 cttttagttc ttgcttacga taactacgag ggagaaaagc acgaatctca tcttcattaa
     8041 aaccgatttg catacgcttg gcatcaataa taattggacg acgcaaaaga ctaggatact
     8101 gctcaatcaa atgaagcaat tccgataccg aaatactctc tacatcaata ttcaattttt
     8161 gaaaaatttt tgaacgagtt gaaatgatgt catcagtacc attttcggtc aaggaaagga
     8221 tgtgttgcaa ttcttttctt gttaaaggac tggtcataat attgtgttcc acaaagggaa
     8281 ccttatgttt ttctaaccag gccttagcct tacgacatga tgtacagctc ggtgatagaa
     8341 atagtgtaat catgcttttc tcttcttatc tatactttgc tacttctatt atacaaaaaa
     8401 ataaagcgct tgactaggga tttttagaaa aaaagcctat tttttcaaga aaaataggct
     8461 ttttgcgaac gattgacaca attggatttg gttaattcac tcttaacgat ggttttaaac
     8521 gatatatatt tttatatatg taaattaaaa acatctttcc tttcacttcc tacgactttt
     8581 cagatacaga tagccaaaga agttttcata gagggcaaaa aagaggagga aggcatgaag
     8641 aaagaaggtc tctggcaaaa tcataataac aggatccttg gctggatcaa aaagccaggt
     8701 atcatctccc acaaagagaa tttgatggaa aagagtaaag aattggtcaa aaccaatcaa
     8761 aactccccca agtccaatca tcacaggtaa gactactaga gccaggagac tttttcgata
     8821 aagagacaaa aagtcctttt tcacaatcct attgacaaag acatagaaac ttggcagtgt
     8881 cactagagct actagctgaa ccaaatgaaa gagattcttg accactgcga aatggtgcag
     8941 accagctgct gacgaacgaa aatcaggcat ctgtaagacc tgactaaaag gattggtcag
     9001 ataattcatc aagatatgaa aattgtattg aatggtttct ggttttagat agactcgatt
     9061 cgttaagttt agccactgaa tctccatagg atagaaaatc caagccagat aaatggtcag
     9121 aaggattgag agggagagga gaaagagcat agagccccaa aaggtcaatt tagttttcat
     9181 caaaatccca ctccgcaagg ctagaaacca catgtgtcgg tgcgattagc aggccagcta
     9241 cttcttctgc cttagtaaaa cctgtcgtca ccaagagcgt tggaatgcca ttgtcaatcc
     9301 cagcccggat atcagtcaaa taattgtccc caaccatgat taactcttca cgttccaaac
     9361 ctaagtgctc aaccgccttg tccataatga tggcatttgg ttttccgata taaaccggct
     9421 tcactcgtgt cgctacttca agcagcgtaa tcagtgagcc agcacctggc aaaagaccac
     9481 gttccgtcgg gatgttgagg tcaggattgg ttccgataaa atgggcaccc ttttgaatag
     9541 caagagttgc tgtggcaaat ttttcatagt cgacttgcca atccagacca actaccacgt
     9601 aggcagggtt ttccttgtct tccacataac cagccgcctt gatggcttcc ttgagtcctg
     9661 cttctccgac gacatagaca gtcttttcaa gccccaaatc attcatatag tcgatggttg
     9721 ccaaagtcgc tgtgtagaca gtcgatagag gcgtatcgat attaaaattc tgagccaaca
     9781 tctccttaac actctctgga gtgcgggttg tattgttggt tacaaagaga tagggaatgt
     9841 cccgcttttg caattcatga acaaaagtct ctccagcagg gattcggtct ttccccttat
     9901 aaatggttcc gtctaaatca attaaatagc ctttatattt catctatttc tccctaaacc
     9961 ttttttattt cttgccaagt aatgattgct tgggcattga taaccccatc acttgtaatt
    10021 tcatgcttgc tttccagtcc agtccgttca acagccgatg taatcacccc acctggtcga
    10081 acttccttga catacttgag gttgattttc ttgggaatat agtgggtcaa aaaatccgct
    10141 cccatgacct caaaaatcca gtccaagtat ttactgttat tgacatgacc attcatatcc
    10201 aagtcgtaaa aacgaacatg gtaatccttg ctgatcggct cttccaagga ttcatacttc
    10261 ggtccacgga taagtttttt atcaaaatca gactggtaag gagccacaat ctcaggttca
    10321 acagcatgga cttttcgact gtcgcggtcc atgagaacaa aggtcgccat catatggatg
    10381 agctcctgcc ccgcttcatt ataaatagta aagcgacggt agcaaaaaag tcgattgtag
    10441 ctcaaggctt ccgtttcgat ggtaatttct tccgcaaaac gaggcaaacg aaccacctca
    10501 atatcatatt ctgcgataat ccagaccaga ttatagtctt ccaaaatggc cttatcacta
    10561 actcccagtt caatcgattg catccctgaa acttgcagtg acagcaaaat cacatctgga
    10621 agtttgatat gaccgttcat atcagccata tcaaaaggaa tttttatttt catttgataa
    10681 gttaagcaca tgatcctact ccaaaataaa tcgttctgct acagtatctc ccaaaaagag
    10741 acctctcttt gtcatgcgaa cgtggtcacc ctcaatctgc atgaggcctt gttgaaccaa
    10801 atctctgaca atttctccat aaagtccagc aaaagactgt ccaaattttt cctcaaatcg
    10861 cgccatggaa accccggatt tcttgcggag tcccaagaac atttcttctt ccatttgctc
    10921 cttttgactc aggtgatctt ctgtaataca agcattgcct tcctcaaccg cactgagata
    10981 atgacgaatg ggaccatgat ttttatagcg tactccattg acataaccag atgcccctgc
    11041 accaatacca tagtattcag cattgtccca gtacatgaga ttgtgacgac tttcaaagcc
    11101 gggtttggag aaattagaaa tctcataatg ctcaaaaccc gctcgctcca gctctgcaat
    11161 gatgtactca aacatctccg cttctagttc ctccttaggc agaggcaatt tcccacgtcg
    11221 catccggttc ataaagaccg tatggttttc taaaatcaaa ctatacaaac tcatgtgggg
    11281 aatatccagt ccaatggctt tagccacatt ttcctttact tgctccatgg tctgaccagg
    11341 cagagcataa atcaaatcaa tggagatgtt gtcaaaacca gccagtttca ggcgatcgat
    11401 attttcataa atatccttct ccaaatgact gcgcccaatc tttttcaaca tcttatcatc
    11461 aaaggtctgg acacctagcg aaacacgatt gacagccgaa tttttcaaaa cagctatctt
    11521 atccgcatcc aaatcgcctg gattggcttc aatggtcaac tcttccaaga cagacaagtc
    11581 caagttttta gtcaagccat tcagtaacac ctccagttgc ggagccgaca gggctgtcgg
    11641 tgttccacca ccgatataaa gggttgacaa cttttcaata tcataagaac gaaactcttc
    11701 cagcagatgc tctaaatagc tgtcgactgg ctgatttttg atgaagacct ttgaaaaatc
    11761 acaataataa caaatctggg tacaaaatgg gatgtgcaca taggctgacg ttggtttttt
    11821 ctgcatagta attattatac cacaaagact agattccaga taaaaatcac catccccaga
    11881 tacatagtcc gtccggagat ggtgatggtt tattcttctg ttatatcaat cacaatctct
    11941 tctgagtcat caagagcttc ggctttttct tgccattgct ccttgagatt atttaattga
    12001 ttttttgatg cttctgtcgc ttgaaaagca taggatttag cttgagcaag tatactgtcc
    12061 acagtgattt cacctgactc aacctgttct tttgttttca gaacaaaatc tgtagcctgc
    12121 tccttaactt ctgtcagttt ttcacagact tgctccttgg catactccgg atcttctctc
    12181 aaatcatcta gaaaatcttg agcctgactg caaacttgtt tgcccttatc acttgttaaa
    12241 aacaaggcaa gagctgcacc tgaaacggtt cctaaaagga ttgaggataa tttacccata
    12301 aggattctcc ttttttattt tttgaaaaat ttacttgcaa gacgaagagc tgacagactt
    12361 gcaccagtct tgagtgtttt tgaaccagct gatgaagctt tcttgctcaa gacacgcgca
    12421 tggtcattga ggtctgaaac agatagagat aaatctgcaa cagcactgaa gagtggatca
    12481 atcgtagcca ccttgacatt gatatcatct gccaagacat tgaccttagc caacaactca
    12541 ttggtgtgat gcaaggtcac atccacatct gaagtcaagg ttttaatcgt cttttctgtt
    12601 tcatcgatga cacgaccaag cttttgtaca gtaatgatca gatagaccaa aaagacaatc
    12661 aaagctaggg caacaagaat atatgcaact tctaacattt agttttcctc ctctgtaata
    12721 tagtaagggg ccttctttcg attttgataa ataacgatca ttataccgag accgataaag
    12781 acaactgaca gccattggga cactcgaagg ctgaagaaca tgagactatc tgttcgcata
    12841 ccttcgatga ccatacgacc gaaaccatac caaatcaagt aaaaggccgt gatatgacct
    12901 cgtctgagac tcttccattt ccgtctaaaa atcagaatca aggcaaagcc aagcagattc
    12961 catagagact cataaaggaa agtcggttga cggtagctcc cctcaatata catctggtca
    13021 cggataaagc caggtagata atccagatta tccactgttg caccataagc ttcttggtta
    13081 aagaaattac cccaacgccc caaactttga gcaatcataa cgctaggcgc cgcaatatct
    13141 agaaaatccc aagtattgat gagtttacgg tcagcaaaga tatagagcac aagagcccca
    13201 gttatcaaac caccgtaaat ggccaaacca ccattccaaa tggcaaaaat ctctcctaaa
    13261 ttctgactat agtaatcaaa tcggaaaata acatagtaga gacgagctcc taaaatagcc
    13321 aagggaaagg ctactaagat aaaatctaaa atatcgtctg gtatgatctt ctttctaggt
    13381 gcttctttca tggtcaaata aaccgcaaga atcaagcctg tcacaataca taaggcatac
    13441 caacgaatgg ctaggggtcc tagttgaata gcaattggat caagcatttt gcacctcatt
    13501 tcgagctatg agacttgtca gtcgttcgtc gaacaaacgg gtagcatcaa agcccatttc
    13561 cttggcacga taattcatgg cagctgcctc aatcacaaca gagatattac gacctgtttt
    13621 aactggaata cgaatacgag gaatggctac gccagaaact tcaagttcct ctgcattgtt
    13681 tccaagacga tcaaaggtct tatgcgtatc gtaattttcc aaatagacag caagctgaac
    13741 ctgtgaagaa tccttgacag cactcgcacc gtagagactc ataacatcga taataccaac
    13801 cccacgaatt tcaatcaagt gtttcaaaat ttcagctggt tcaccccaga gagtaatctc
    13861 atccttggca aagatatcga cacggtcatc ggctaccaaa cggtgaccac gtttgacaag
    13921 ctcaagacct gtctcgctct taccaattcc actatctccc tgaatcaaga cgcccatccc
    13981 ataaatatcc atcaagacac cgtgcacact ggtacgttct gccaaacgag aatccagata
    14041 gctagataac tctccagata aacgactggt agctgcacgg ctggttaaaa tagcaatctt
    14101 acattctcta gcagccttta acatctcctc tggaaccacc aaaccacggg caacaatgac
    14161 tgctggtgtc tcaggtagaa acattttttt caaaacttca taacggctgt tggaaggcat
    14221 gctgatcaga taagaccact ccttcatccc caaaagttga atccgctctg gtgtatagta
    14281 gtcaaaatag cctgtcattt caagaccagg tcgcgtaata tccgctgtat tgatttcctt
    14341 ttcaagcaat tctagttcac catagacaat atctagtcta agcttttcaa tcacttcttt
    14401 tactaaaacc gacatcattt cctccttcaa atgaattctt ctctctatta tatcaaattg
    14461 taggaggaag tttcagattt ttgcaggctt tggagtattt atttaaaaga aaaaggacta
    14521 gatttctcta gcctttcatt tcaattagaa ttttttacgt ttacgagcta cttctgattt
    14581 acgtttacgt tttacagaag gtttttcata gaattcacgt ttgcgtgttt cttgaagagt
    14641 accagcttta gtaaccgcac gtttgaaacg gcgaagtgcg tcgtcaagag attcattctt
    14701 acgtactact gttttagaca tttttttcac tcccttcaag ttcaatatct atttcattct
    14761 acacgtaaaa ggaataattg tcaagcaaaa atgcggttta attttgattt tttcttccat
    14821 ttatacaaga gttgaaagcg ttcaatagaa cattgctttt ttatttttcg agtaagctaa
    14881 gcgcttcagc atctgcgatg atggttacat cagggtgatt ttggaggcta cttgcaggta
    14941 ggttctcagt cactgggcca gatactgttc cggcaatggc ttctgctttc gactcaccat
    15001 aagcaaaaag aataatagac ttggcatcca aaatgttttt aatccccatt gaaatagctt
    15061 gggttgggac gtcttcaatc ttggcaaaga agcgagcatt ggcttcgata gtagactggt
    15121 caagttctac tagatgcgtt tgactgtcaa atggagtacc aggctcatta aagccgatat
    15181 gcccattgcg accgattccc aagatttgga ggtcaactgg atggtcagcc aaaatttggt
    15241 tgtagcgttc aacttcagct tcagcattgt ccttaacacc acgaggcaag aaactttctt
    15301 taaatggttt ttggttgaac aagttttctt gcatgaagta acgataagac tgtggattgt
    15361 ccccatcaag gcccacatac tcatcaaggt tgacactggt tagatttgaa aaatcaaggt
    15421 tactctcaac aatttccttg taaaattcaa gtgggctact tcctgtcgca agtcctagag
    15481 tttgagctcc attagccaat ttttccttca aaatctcaaa agcaactttt ccaccttgaa
    15541 cttggttttc aactttaata actttcatct tttcatcctc catttctgtc tatatttatt
    15601 atatggtata gaccaatttt tgtcaagtga aaacgattta atttctaaaa aaagagcgtc
    15661 caaacttgaa acgtgttata atggatagga ttagaactta gaaagaatga acagatatat
    15721 gaatacagct gattttgatt tccacttgcc agaggaattg attgcccaaa cgccccttga
    15781 aaaacgtgat gcctccaaac tcctcatcgt caaccgtgag acaggagaaa tgcaagataa
    15841 acacttccac tctattattg atatgctgga acctggtgat gcccttgtca tgaacgacac
    15901 ccgagttctc cctgcccgcc tctatggtca aaaagtggag acaggaagtc atgtggaact
    15961 tctcctcctt aagaacacta gtggagacga gtgggaagtt ctggctaaac ctgccaaacg
    16021 cctcaaggtc ggtactcgta tcagctttgg tgatggccgc ctcagcgctg tcgttacaga
    16081 agaattgacc cacgggggac gcattgtccg ctttgaatac caaagaattt tcctagaagt
    16141 cttggaaagt ctgggagaaa tgcctctgcc accttatatc cacgaaaaat tagatgaccg
    16201 tgaacgttat caaaccgtct acgccaagga aagcggctct gctgcagcac cgactgctgg
    16261 tcttcacttc accaaagaac tgctggcaga aatccaagct aagggtgtcc atctagtcta
    16321 tctgactctc catgtcggac tcggaacctt tagacctgtt tctgtggata atctggacga
    16381 acacgaaatg cactcagagt tctatcaact ttctgaggaa gctgcggcca cccttcgctc
    16441 tgtcaaagaa aatggtggtc gtgtcatcgc tgtcggaacc acttctatcc gtaccttgga
    16501 aactattggt tccaagtttg atgggcaaat ccaagcagat tctggttgga ccaatatctt
    16561 tatcaaacct gggtatgagt ggaaggtcgt ggatgccttc tcaaccaact tccacctgcc
    16621 aaaatcaact ctggtcatgt tggtttctgc ctttgcaggc cgtgaattag tcttagatgc
    16681 ctaccaccat tccatccaag aacactaccg cttcttcagt tttggtgacg ccatgtttat
    16741 ttattgagaa agaatttctc taaatcttct aataccaata aatcgctaag atattatttc
    16801 aaagaacatc tacaattgaa actctagcta gctgtagaag acgcctagta cattgaaatt
    16861 aaaatgcaag acacatgagt agcaccatat ttgttgctct tatctgtcct cgcaagagac
    16921 tattatgagt tatttcagaa tcattcacta ctttgaccct gactctcctt agtctcaaaa
    16981 tcaaagactt atactcttca aaaatctctt caaaccgcgt caacgtcatc ttggattata
    17041 tatgtgactg acttcgtcag ttctatctac aacctcaaag cagtactttg agcaacctgc
    17101 gactagtttt ctagtttgct ctttgatttt cattgagtat taaacaaaaa gtgaacaaag
    17161 ctgaattcta atgtacagaa gactaggctt gttcactttt ttatagtcgc tataagatga
    17221 ccttatctat agctttttat atataattat atattcagac atactattat caattttgtc
    17281 gcagggagga atctgttaac gcacccattc accattatca ttgactctat agccatctat
    17341 acttgtattg accgctaact cacccgatgt atttacataa taccatttac caccaacttg
    17401 gaaccattga ttgactttca tagaaccgtt gctgttgagg tagtaccatg aaccattaac
    17461 ttgtacccaa cctgttgcca tggaaccatc agtattataa aaataccaca taccattttc
    17521 ttgtttccag cctgttgttg gagcaactgc tttagctggt tctactgcta catctgttcc
    17581 ttggttagat gtaacagata caggatacga aggaatagat gattgctcag gaacaacaac
    17641 tttttcaggt tctctcgtcc ctctccttat acgtcttttt accatctctt tagtaatttg
    17701 acgagaagta gtttcttcaa ttgttccatc acgttcatct acagcataga ttgtagtaag
    17761 agtaatttta ccaatttctc ctacttcttg acctttatca agagttgggt catcgagata
    17821 ttctgtttcg attggaattt cttggacaag aacttggggc ttggttcctt ttttaacaac
    17881 tcttgtttga gagtcttctt tttgacttaa agtactctca gttacttgtc cactctttcc
    17941 atctacatca taagttatcg ttgtaactgt tttcccattc tttcctagag taatctcttg
    18001 ctcctgtcct gcagaaaggt cattgtctgc ttcatattta gtagtaaatg gaacaagaac
    18061 ttcttcaacc ttgcttttag ctggaacttt gataactgta tccgtggctt cttttctatc
    18121 aacagtaacc ttttcggtaa catgaccagt ctctggatta acatcgtagg tccttgtcgt
    18181 agttacatag ccatcctctc catcaattgt aacaggattt tcactacggt cttttgtttc
    18241 atctttttca taacgaattc gcgtacttga aattttcttg gttactacct taggtttagt
    18301 cgctactttt acaataatac tcccattgtc agcgtcatca tactctattc cctcttcttt
    18361 atctctagta tcatctctga catattgaat cccatcagca gcatgaacaa aacttgtatt
    18421 cagattcctc ctaaaaataa agttagcccg attaccgcag aaccaaaaat ctttccgagt
    18481 ttacgtaatg catagcgctt attagtatta gattttgcca ttacatccta cttctagtat
    18541 agcatctttt ctatcaaacg ttaaacagta tactttatat atcaaataga cttagaatga
    18601 tatattgatt attgaactaa cactttaact atatcgtaat caatctcata tataaaggat
    18661 tgcagacatc ttatctaaat acatgctaat atatttagat acaaacattc caacttgata
    18721 attttcactc atctttcatc attccttata
//
LOCUS       DAVLVZ010000034        18545 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000034 DAVLVZ010000000
VERSION     DAVLVZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.034"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..188)
                     /locus_tag="VYN77_001245"
     CDS             complement(<1..188)
                     /locus_tag="VYN77_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1558705.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADFISKSQLSRFERGENEISL
                     STFFELLSNINVSIENFC"
     gene            complement(201..974)
                     /locus_tag="VYN77_001246"
     CDS             complement(201..974)
                     /locus_tag="VYN77_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1558706.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGTGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLAS
                     LFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
     gene            complement(1420..2175)
                     /locus_tag="VYN77_001247"
                     /pseudo
     CDS             complement(1420..2175)
                     /locus_tag="VYN77_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(2313..3935)
                     /gene="groL"
                     /locus_tag="VYN77_001248"
     CDS             complement(2313..3935)
                     /gene="groL"
                     /locus_tag="VYN77_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1558707.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(3951..4235)
                     /gene="groES"
                     /locus_tag="VYN77_001249"
     CDS             complement(3951..4235)
                     /gene="groES"
                     /locus_tag="VYN77_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1558708.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(4505..4900)
                     /locus_tag="VYN77_001250"
     CDS             complement(4505..4900)
                     /locus_tag="VYN77_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1558709.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(4978..5739)
                     /locus_tag="VYN77_001251"
     CDS             complement(4978..5739)
                     /locus_tag="VYN77_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1558710.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(5772..6398)
                     /locus_tag="VYN77_001252"
     CDS             complement(5772..6398)
                     /locus_tag="VYN77_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1558711.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(6414..6731)
                     /locus_tag="VYN77_001253"
     CDS             complement(6414..6731)
                     /locus_tag="VYN77_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1558712.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRVDRDQYIDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(6728..7027)
                     /locus_tag="VYN77_001254"
     CDS             complement(6728..7027)
                     /locus_tag="VYN77_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1558713.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(7277..7465)
                     /locus_tag="VYN77_001255"
     CDS             complement(7277..7465)
                     /locus_tag="VYN77_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEV1558714.1"
                     /translation="MKASTWVYDKGEWYYVSSSGAMLANDWVKDNGKWYYLASSGKML
                     RNTYTPDGYYVGNSGAWQ"
     gene            complement(7716..8390)
                     /locus_tag="VYN77_001256"
     CDS             complement(7716..8390)
                     /locus_tag="VYN77_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558715.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            complement(8396..8842)
                     /locus_tag="VYN77_001257"
     CDS             complement(8396..8842)
                     /locus_tag="VYN77_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1558716.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(8956..9459)
                     /locus_tag="VYN77_001258"
     CDS             complement(8956..9459)
                     /locus_tag="VYN77_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1558717.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(9456..9818)
                     /locus_tag="VYN77_001259"
     CDS             complement(9456..9818)
                     /locus_tag="VYN77_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEV1558718.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFLQAPYYVPERIAITGKICGVHIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(9884..11743)
                     /locus_tag="VYN77_001260"
                     /pseudo
     CDS             complement(9884..11743)
                     /locus_tag="VYN77_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(11782..12231)
                     /locus_tag="VYN77_001261"
     CDS             complement(11782..12231)
                     /locus_tag="VYN77_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1558719.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIDQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(12355..13071)
                     /locus_tag="VYN77_001262"
     CDS             complement(12355..13071)
                     /locus_tag="VYN77_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1558720.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(13952..15367)
                     /gene="ply"
                     /locus_tag="VYN77_001263"
     CDS             complement(13952..15367)
                     /gene="ply"
                     /locus_tag="VYN77_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1558721.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(15378..15710)
                     /locus_tag="VYN77_001264"
     CDS             complement(15378..15710)
                     /locus_tag="VYN77_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558722.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(15781..16389)
                     /locus_tag="VYN77_001265"
     CDS             complement(15781..16389)
                     /locus_tag="VYN77_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558723.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(16402..16860)
                     /locus_tag="VYN77_001266"
     CDS             complement(16402..16860)
                     /locus_tag="VYN77_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1558724.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            16985..17384
                     /locus_tag="VYN77_001267"
                     /pseudo
     CDS             16985..17384
                     /locus_tag="VYN77_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(18149..18409)
                     /locus_tag="VYN77_001268"
     CDS             complement(18149..18409)
                     /locus_tag="VYN77_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558725.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFFDG"
BASE COUNT         5511 a         4132 c         3269 g         5633 t
ORIGIN      
        1 ttacaaaaat tctctatcga aacattgata tttgataata attcaaaaaa tgttgaaaga
       61 gaaatttcat tttctcccct ttcaaatcta gaaagttgag attttgatat aaaatcagct
      121 gcagcatact ctaaagaata tccgtttaat tttctaaaat atcgaaaagc ttctccgtaa
      181 tttttcacac tttcattccc ttaactttct tttcgagcga taaacatacc atattcttta
      241 ggaatgatca acataccatt attaaactca ctttccaatc ttttacgtat tatttcctct
      301 tctagtgaac ctatttctga aattccttta aacgattgga tgtatttaac taggtcatct
      361 atactagtta cctgtaactc atcatcatag agtaatgtat cgacagagtt aaaataccta
      421 ctcaggtacc ttttaccatt ttgtaaagta aacgttctat tttctaaatc ttgattaacc
      481 tcatctttaa ataaacttgc caaataattt acaactccat tttcaccaaa tgtagcacag
      541 taaaaaatgc ctccggtttt taagacccta ttcacctcag agagagcttt aggaatatca
      601 ttcacatgat gtagaagcat attagcaata acaatatcaa aagtttcatt ctcaaaagaa
      661 atcttttgaa tatccattat ctcataattg acattgtttc tatttccaat cactgacttc
      721 gttgatttta ccatatcttt agaaaaatct gtaacaatca actgcttcat tttatctata
      781 gaatcactat tacttttcca caattctcct gtaccacatc ccaattctag aacctttact
      841 tcatcagtga tttggtagtt agaaaatatc caattattaa atcccaattt attttttgaa
      901 tacttcttat gcaattctac tcgaatatca agattatcat gacttttata ttgttcaatt
      961 actttatcaa tcattttaaa tgacctccac tctataattt ggcattatat cacaagaata
     1021 aagatagtgt taaataagtt atataaatgc aaaaaaggaa tattccgtta tagcagagtt
     1081 gtaacacatt tctataaaga aatttattcc tacgactcag tttggcatgc ttaacttcct
     1141 aactcaaaaa caaagcatcg attaatttat ccgttcttct tttgaaacag atttgaactg
     1201 ataattgctc gttttaattt aaaaagctct tttgagcaaa acttcatttt tttaagagtc
     1261 caacaatttc tgtctatcgc ttttctacta gcaaaaaacc taccttgagg caagttttca
     1321 tttatatatc taatttcgta acagtatagt aaaatgaaat aaggacagtc aaatcgattt
     1381 ctaacaatgt tttagaagta aaggtgtact attctagttt caatctacta taaatttgat
     1441 atgagaatag tctttctcaa catccttatc caacaagcat gctgtggcgt ccttcttaac
     1501 ttaaaccagg tcaatattct gggcttgggc tgcatagacg cagccacaat aacattgacg
     1561 atagatatca tactcctcac acatctctac tgaacgtttg tagccttgat ttttcttgaa
     1621 atcgttggga agatagtggg gcgtgtaaat tttttgcaca tcgattccga tgctattgat
     1681 agtttgagaa ttcttatgag gactgatggt caaggctgaa ccaaagtagt caaagcccaa
     1741 gtccatagcc acttgcgctg ttttatccag tcggtagtca aaacaaacct tgcaacggtc
     1801 gccaccttcg ggctcctcct ctagccccct aactagtttt cggtattaat tgggttcgta
     1861 gggagcttct aggtactgaa ccgtatttcc tgtccgctca ttaaaatcac taacaaattt
     1921 cttggtgaca tagacccgct tatggtattc tgccttggga tggatattag aattggcaaa
     1981 atagatggtc acatctgcat acttggtcaa atattctagt gtataggtac tacaaggggc
     2041 acagcaaaca tgcacgagaa tggttggccg ctactcattt ttttcccatg cttgtaccat
     2101 tttctgcatg acacggtcat aattaatctt ctgattgggg gtcatcttgc acagaatttc
     2161 ttctacatcg atcatgttct tctccttttt ctagtcttat tttatcatat aagttaggag
     2221 agctcaaatc aatttagaag tgaatattat aaaaagaagg gttagtcatt ccctcctttt
     2281 gtgtttttta taagttgttt tctatagaaa gcttacatca tcccgcccat catacttgga
     2341 tccattgctg gagctggggc tactggttct ggtttattgg ctacgactgc ttctgttgtc
     2401 aaaatcaagc tggctacaga tgctgcattt tgtagggctg aacgactcac tttaactgga
     2461 tcaatgatac cttgatcaat catgttaacc cactcgccag ttgctgcgtt gaatcctata
     2521 ccaagctcag catttttcaa acgatcgata acgatagatc cttcaaatcc tgcattgtga
     2581 gcaatttgac gaacaggttc ttccaaagca cggagaacaa tattacgtcc tgttgcttca
     2641 tctcctgtca attccaaggt agcaacagct ggaatcacat tggcaagagc tgttccacca
     2701 cctgaaacaa taccttcttc aacagctgca cgagtagcgt tgagggcatc ttcaatgcgg
     2761 agtttcattt ctttcaactc agtttcagtt gcggctccga ccttaataac cgctacacca
     2821 cctgacaatt tggccaagcg ttcttgcaat ttttcacggt caaattcaga agttgtagtt
     2881 tcgatttgag acttgataac cgcaacacgg tgagaaatcg cttcaggatt tcctgcacct
     2941 tctacaataa ccgtgctatc tttgtccacg gtcactctcg ctgcttgacc aagagcttca
     3001 attgtcgcat ctttcaactc aagaccaagg tcttctgtga taactgttcc gcctgttaag
     3061 atggcgatat cttcaagcat ggctttgcga cggtcaccaa aaccaggtgc cttgactgct
     3121 actacgttga aggttccacg aatcttgttc aaaacaagag ttggaagagc ctcgccatcc
     3181 acatcatccg caataatcaa gagtggacga ttgctttgga gaatgctttc caaaagtggc
     3241 aagatttctt ggatattgga aattttcttg tctgtaatca aaatgtacgg attttcaagg
     3301 tcagccacca ttttttcgct atctgtcacc atgtactgtg aaaggtaacc acggtcaaac
     3361 tgcattcctt ctacgacttc aagctctgtt tccataccac gtgactcttc gatggtgatg
     3421 acaccgtctt tgccaacttt ttccattgct tcagagatgt actcaccaac tttttcagaa
     3481 cgagaagata cggctgcaac ttgagcgata gcttctttat tggcaacagg gatggcgttg
     3541 tttttcaaag cttcaactgc tgcggcaact gctgtttcaa tcccacgacg aataccgatt
     3601 ggatttgcac ctgctgtgac gtttttgatt ccttcacgga cgattgcttg ggtcaaaaca
     3661 gttgcagttg tagttccatc acctgcgata tcattggttt ttgaagctac ttctgatacc
     3721 aatttggcac ccatattttc aaaatggtct tctaattcaa tttctttggc aatagtcaca
     3781 ccgtcattgg taatcaatgg tgagccaaat gatttttcaa gaacgacatt gcgacctttt
     3841 ggtcccaaag ttactttaac agtatctgca aggatatcga caccacggac catggctgaa
     3901 cgggcatctg atgaaaattt aatttctttt gacatactta ctttctcctt ctattcttca
     3961 atgattgcca agatgttagc ttcgcctacg atgatgtact tttcatcgcc atctttgaca
     4021 tcaagacctg cgtgggcttc aactaagaca cggtctccag ttttaacact tggagcaacc
     4081 aagtcaccgt tcaaggtacg aacaccttgt ccagtagcca caacttgggc tgttttggtt
     4141 ttttcttggg ctgaacctgc gagaacaaag cctccaacag tttgttcttt ttcttctact
     4201 tttaagacca cacggtcccc taatggtttc aacatctgat ttcctccata atgagataga
     4261 tattattagc actctttata cctgagtgct aattcatagt tctattgtat cacttagtca
     4321 gaaataatca agaaaaaagt ctgactttct caagataaaa agcctgagac caactcagac
     4381 tttttaatgt ttatactcaa tgaaaatcaa agatcaaact aggaagctag ccgcaggctg
     4441 ctcaaagcat tgctttgatg ttgtggataa agctaacgtg gtttgaagag attttcgaag
     4501 agtattaaaa tggcaattct tcctcttcca agactaaatc tgccaaatct tggcctgcat
     4561 tattttcacg catggcacgt tgagcacgac tttccaagag ttggaatcct gttacaagta
     4621 cttcggtcac gtagttcatt tggccatttt tctcaaagcg acgggtacgc aattctccat
     4681 caacggaaat gagactacct ttggttgcgt agcttgccaa agtttctgct agtctgcccc
     4741 ataggaccat attgacaaaa tcagcttcac gttcaccgtt ttggtctttg taacgacggt
     4801 tgacagcgat agttgctcgc gctaccgact tgtcattgtt ggttttgtgc aattctggtg
     4861 tagacgttaa acgcccaatc atgataactt tattatacat attttcttcc tcctacttat
     4921 ctattcgtag gaaatcaaaa aaagttacag aaatttgtaa cttttcgagg aaatttctta
     4981 ttttttatga atcatgaaac ctgtcgcctg ttgattggcc ataatggtca tatctgtaat
     5041 ctgaacacgg cgaggctgac tggtcacata gactactgtg tctgcaatat cctgagcttg
     5101 caaggcttct attccttggt aaacggacgc agctcgctct ttatcaccat gaaaacgaac
     5161 agttgagaaa tctgtttcga caatcccagg ctgaatggtt gtcaccttga tatccgttgc
     5221 gatggtatca attcgcagtc catccgaaaa ggtcttaacc gcagccttgg tagctgagta
     5281 aacagcggca ccagcatagg cgtagattcc tgcggttgac cccatattga taatatgacc
     5341 ttgattgact tttatcattg ctggcaagaa acagcgagta accgccatca gacctttaac
     5401 attggtatcc aacatggtca acatatccaa ctcctcatag tcttgatagg gagccaagtc
     5461 aagagccagt ccggcgttgt tgaccaagat atcaatcctc cctatcgttt ctagaatatc
     5521 agagcaaaca gtcttcacca tgaccatatc cgtcacatct agtggaaaag tccagacggt
     5581 ttgatttgga aagcttactg caaactccga cttgaggatt tctaatctgt ctatccgtcg
     5641 tcctgttaga acgacatcct caccctgctc cagataagca cgcgcaatcg cttcaccgat
     5701 tcctgaggtt gctcctgtaa tcactacatt ttttgccatc ttatttcctt ctatcagatg
     5761 ctgacaagtt cctaggcagt ccagtgtttc gctgggtcga acggtgttcc gacaacttgg
     5821 tcttctgata attcaagcac cccacgtttt tgtggagcat ttggcagatg caattcacga
     5881 ggactgcaca tcataccaaa actcttttca ccacgaagtt cacctgggaa aatgagattc
     5941 ccttttggca tcatagctcc aggaagcgcg acaatggttt tcaaccccac acgcgcattg
     6001 ggagctcctg caacgatttg tacagtctta tcacttgcga ctgcaacttg gcagatgttg
     6061 aggtggtcac tatctggatg ggctaccatc tcaacaattt caccgacaac aaacttaggt
     6121 tccttatcat taacaatttc ttctgtaaaa ccttccgcct gcaactcttg gttcaaacga
     6181 gcgacttgct catctgtcaa aaagacttga ccgcgctctg caatttcaaa taaacttgaa
     6241 acttcgaaaa tattccaagc cactgtttcc ccattatctt tgagaaaaac acgggctacc
     6301 ttgcctttgc gctccacatc cagtttggca tctccgctat ttttcacgat gaccataagg
     6361 acatcaccga catgttcttt attatatgta aaaatcattg tttccttttt ctcctatttc
     6421 agtcctgcta aaaagtcatt gatttgttgc ttgcttttac ggtcgcgatt gacaaaacga
     6481 ccgatttcct tgtccttttc tagaacaaca aggctaggga ttccatatac atcccagagt
     6541 ttggccaaat ctatatactg gtctctatcc actcgaataa aggtaaactc tggattagtc
     6601 tcctcaattt ctggtaaggc aggatagata taacggcaat cgccacacca gtccgccaca
     6661 aaaaggaaga ccttcttacc gtctttttct actaaagatg ccaattcttc taaacttgct
     6721 ggttgtatca taagacttcc tcctcataga ctaggtcttc attttcatag acaaaagtat
     6781 aatgacggcc atcctcaaaa atgacgccac caagcaagct ctctaaactg ctttcataga
     6841 cttgaacata gagggtcgca atttccccca tgtctgaaaa atggtctcgt acaatcgctg
     6901 tcaactcttc ctgagtcttc atgagcttat ggtcatctgc aacttttttc gtagcaaggg
     6961 caaggctccc aataccaagc agagccagac ctgccatcca cattttttta gctttcatac
     7021 cattcatttt aacacaaaaa aggctttagg acaaatgagg aaacagcaaa aaagcaagta
     7081 aaaagcctct tcctttaagg aaaaggactt cttatactca atgaaaatca aagaccaaac
     7141 taggaagcta gccgcaggct gctcaaagca ctgctttgag gttgtagata gaactgacga
     7201 agtcagctca aaacactgtt ttgaggttgt ggatgaagct gacgtggttt gaagagattt
     7261 tcgaagagta ttattcttat tgccaggcgc ctgagttacc aacgtagtaa ccatcaggtg
     7321 tgtaggtatt gcgaagcatc ttacctgatg aagctagata ataccacttg ccattatctt
     7381 tgacccaatc attcgctagc atggctccag aagaacttac ataataccat tctcccttgt
     7441 cataaaccca agtgcttgct ttcatggctc ctgagcaatt aaaggttcaa aaaactgtcc
     7501 aatatcattc atttttttaa agcattttac actacatcat ccacgctgta ggacatagat
     7561 acatcaaacg taagagccaa aatatcgaaa aatatacaac ttttaaaggc aacatttcat
     7621 tttttaaagt ctaacaacca tagaactgaa atcagagcaa ggcatcaact tcctactctt
     7681 agtacgtcta ttttgatggt tgaagtgaag attttttatt taacgtagac aagctccaac
     7741 ttcccaaaag ccacatctcc acggataatc aaggttttgc tggttggggc tacaggagcg
     7801 tcagccttag ctgcaccaaa ggaggtttct actttcaaat ctacacgcca gtgttgtgga
     7861 acatagacgg ttgcattccc gaaggccact tcaatattta aagttgcaaa atcacctagc
     7921 atctctgcat tatcatagta gattttagca tccccaaaag cgacttccac ttggtcatct
     7981 acgagatctt gatcttgctt atagaaggtc ccgctaccaa aagcgacttc cttatccgtg
     8041 acgactgttt ttttcccatt gtaccaccat tttttttcat tccagaactt acttgaatgc
     8101 gtcagataac caacaccaag taccaccaag atgctagccc aaataagaga atgattggta
     8161 actggtaaca agtcataagc gtaatttgca atgatgagcg ccagtaaacc tgtaaaaact
     8221 gccgaagtga gatgacgtct aaggatggac tcaatggact tataagcaaa aaaaacaata
     8281 cctagtaaag gccatatttt accatccaaa gaaggaattc caaaattccc ttgcagcaag
     8341 atccaagctg ctaaaaccaa taaaacaata ccaaatgctt tctttttcat gttttttacc
     8401 tcatgtttct tagattttct tttaggaggg attggtaatg ccgtgagaca tgaacctcct
     8461 tgtgcgtctg ataaaaggaa atggtgcccg ttcctgaaaa ggacttgtcc actgagtaaa
     8521 tctgacggat gtttgcgatc gttgacttgg aaactcgatt aaaatagcga ggcaagatag
     8581 actccaactc atagagcttg aggcgaacct cataagcttc cttctggtta tgagcgtaga
     8641 tcttgctccc ttctgtttca aagaagagaa tttctgacag gtctagataa tattcacctg
     8701 tccccttgta aaaagtcaac ctcggagcct ttgactcttg caagagccgt tgcaagtctg
     8761 caatttcatc tgtcaaagtg ggggtcttga tgacaatttc agtctcctct aaattgccgt
     8821 caatctcaat tcgtaacttc atcacatctc ctttctgact ctactatatc aaagctaagt
     8881 taggaataca agggcaaaaa agcaagtggt caatttgaag cagtaagtgg ttgtaggact
     8941 agcttaactt acatgtcaga ataggaagaa aaggaatccc cacaccagac cacaagcata
     9001 gccaaccaag acatccttgg gataatgaac accccctaaa accctcacca accccagcaa
     9061 agctgaaaag atcaagcata ctaagccaat aggtagacta gcatgcagac aggccatgga
     9121 gatgatagtt gcagaaaaga catggcgact gggcatcgac cgtcccgaac tatccttatc
     9181 aagaagggga cagatatccc aagtttcata aggtctcggg tgattgattt tcttacgaaa
     9241 cagggacaag gccacaaaac ctgatgcagg aataagcaca taaaccccaa cttgctgacc
     9301 aagtcctagt tgcaggtaag tggtgactag caagatcaga tagaccaggg gcatggcgac
     9361 tgtcatcaaa cgattgaaac tgcgtaatag gaataaaaga attggacgac tagttagtct
     9421 ggagctgata tttcgatacc attcttgata atttttcatt tatgcttctc atacttttct
     9481 agaacttgtt gagatgtttt ccccttggca agccaatcca ctagcttgtc caaacgacgt
     9541 atttcctgca tcagaggatc atcaatttcc tcaatatgga caccgcaaat cttccctgta
     9601 attgcaattc gttcagggac ataatatggt gcttgtagaa aaaaatctcc gtaagtaaca
     9661 tccctatcca gacaagcaag aacgtcactc acttcatacc ctgtcaacca actggtcact
     9721 tggtgaactg actctgcctt tccacccttt cgctcaactt ttgcaatcaa ggctaagtag
     9781 acagcagcaa atttcatatt gtataatttc tgactcatac ttctctcttt ctatgtgtac
     9841 tagtataaat aagaaaaaga aggccgtcaa gccttttttt gatttattct tctgcttcat
     9901 cttctgtaaa ttgactattg tacaagtcag cgtagaagcc accttgcgcc atcagttcct
     9961 catagttgcc ttgctcgatg atatttccat ctttcatgac caagatcaag tctgcatttc
    10021 ggatgattga caagcggtgg gcaatgacaa aggatgtgcg tccttccatc aaacggtcca
    10081 tggctttttg gatcaattcc tctgtccgtg tgtcaacaga agaagtcgcc tcatccaaaa
    10141 tcaaaagcga tgcatcctta agaagggcac aagcaatagt caatagttgt ttttgtccta
    10201 cagacaaggt cacggtgtca tccaagatgg tatcatagcc atctggcaag gtcataataa
    10261 agtggtgaat tcccacagcc ttactagctt ccatcattcg ttcatcacta atccctattt
    10321 gattatagat gagattgtct cgaatagttc cttcaaagag ccaggtatcc tgcaagacca
    10381 ttgaaaaggc atcatgcact tctgaacgcg tcatagcctt ggtatccaca ccatcaatgc
    10441 gaatacttct cttatcaatc tcatagaatt tcatcaaaag attgacaatg gttgtcttac
    10501 cagccccagt cggcccaaca atggcaaccc tttgaccagc atgagctgtc acagagaagt
    10561 catagtcttg aacattgaca ccgtccacca gaatttctcc tgctgacacg tcgtagaaac
    10621 gtggaatcag attgaccaga gttgatttac cagaacctgt tgacccaata aaggccactg
    10681 tttgaccagt ttctgcttta aagctaacat gttcaataac tgcctccgaa tttgccgcat
    10741 agcggaaggt cacatcctta aactcgacct gacctttgaa gttttcatca gtcagctgca
    10801 cttgaacagg gttttggata gaagaatgca aatctaaaac ttgattaatc cgcttagcag
    10861 agaccatagt tcggggaaga acgatgaaga gtgctcccat gagaaggaag cccatgacaa
    10921 cctacatggc ataagacatg aaaataatca tgtcactaaa gagaggcaga cgcgctatcg
    10981 gagcagcgtc gttaatcaca taggccccaa tccagtaaat cgctacactc aaaccacttg
    11041 aaatccccat catgatagga ttcaaaatag ccataagacg gttgacaaac aaattcaaac
    11101 gggtcaattc atcatttact gctgcaaatt tttcattttg ataatcctct gcattgtagg
    11161 cacgaacgac acgaatacct gttaaactct cacgagtgat actgttcagt ttatctgtca
    11221 acccctgaat caaggactgt tttggaaagg ctagcgtcat caaaacggtc gtcatcagga
    11281 cgttgacaat cactaccaca agtacggccc agagccagta ttctgaatga cctaaaatct
    11341 tcccaatagc ccagatagcc ataattggac cacgcgttac cacttgcaag cccatagtaa
    11401 tcaacatttg aacttgagta atgtcattgg tagtacgcgt caagaggcta ggaattgaaa
    11461 atttcttaat ctctgtctgc gagtaatcca aaactcggtt aaaaatatca cttctcagcc
    11521 tactagtata agaagccgcc actcgggatg caaaaaatcc aactgcaact acggacaaga
    11581 aggcaagaaa ggacattccc atcatcatgc ttgccggctg ccacaactca tctaaattag
    11641 tttcttgact acctagcaaa tccgtaattt tcgagatata ggtcggcact tccaactcta
    11701 gatagaccga aaagcaagta aagagaatgg ctagtaaaat catcccccat tcttttctac
    11761 taattctttt ggctaatttc tttattctct cctcctattc ccttgatatt ttgcctgtag
    11821 ttgaccgaga accttctcaa aaatcagtaa ttcatcttca tcaatatctt ccatcaactg
    11881 cttgtctatg cgttcaaaaa aagccttaac ctgttgcatc tgagaacgtg ctttgtccgt
    11941 cagacgaaca aacttagccc gcttatcaac aggactcgcc tccaattcca ccaaaccatt
    12001 ttgcactata cgcttaacca gattactagc aacagacttg gtaatattga gttcctgatc
    12061 gatatcttta atcaagacca agtcttggtt tttctcgcga ttatccaaaa aatgcacaac
    12121 ctgaccttgc ggcccaccca taaattcaat gccgcaacgt ttggcttcct tttgcaccat
    12181 caggtgaatt tgatgaccaa aacgcttaaa gactaacatc ggtttaccca taatctcccc
    12241 cttctaaata aaaatagttc tctggagaat aattaaattt ctatgagaac tattttcttg
    12301 attaaaaaaa tcccaagtga ttttctcact taggatcatg ttctataggt taaattaaaa
    12361 cccatctacg ttcgtataaa tcttttggac gtcttcgtcg tcttcaagaa cgctgtaaag
    12421 tttttcaaag gtttcaaggt cttcgcctga caattccact tctgactgag gaatcatttc
    12481 caattcagtc acttggaatt cttcaatacc agactcacgg agggcaacga tagccttgtg
    12541 aaggtcagtt ggcgctgtgt aaactgtgat tgtaccttct tgtgcttcta cgtcatccac
    12601 atccacatcc gcttcgagca attgctcaaa gactgcgtcc gcatcttcac ctccaaatac
    12661 aataacacct ttgttgtcaa agaggtaaga aacagaacct gaagcgccca tgtttccgcc
    12721 gtttttacca aaggctgcac ggacattggc tgctgtacgg ttaacgttag aagtcaaagt
    12781 atccacaatt agcatagagc catttggccc aaaaccttcg taacgtcctt ctgtaaaggt
    12841 ttcgtctgtg tttcctttgg ctttatcaat cgctttatcg ataatgtgtt ttggcacttg
    12901 ggcttgttta gcacggtcga taacgaattt caaagctgag tttgattctg gatctggatc
    12961 acctttttta gctgctacat agatttctac accaaatttt gcatatactt tagagttggc
    13021 tccatcttta gccgttttct tggctacgat attggcccat ttacgtccca ttaggaatct
    13081 ccttttttca cattttaatc tttcttatta taacacaagt ttttttgatt ttcactagag
    13141 gaaatggatt ttattagcaa atcaagctag gatagcactt tacctgctaa gatggtcttg
    13201 cctttctatc tttatcaaca gacactcatc cacattcaaa aaacaaacta gaccattatc
    13261 tgcaaataga aagtttcagc caagtttgac aaagtcagct caaattactg tttgaagttt
    13321 gtagatataa gcgacaaaaa caatcatact gcaccttttg ttgacagtct actccagaca
    13381 tatcatagtt caagtaaata ctttgaaatt caacagttct tataggcgct attgtattct
    13441 aagaaatcaa tagaagagtt tctaagcaaa cctctaatac tcaataaaaa tcaaagagca
    13501 aactagaaag ctagccgcag gttgctcaaa acactgtttt gaggttgcgg atagggctga
    13561 catggtttga agagattttc taagagtata atttacgtgt tcctaagatg gagaagttag
    13621 actagtacac tggcacttct aaaccattgc tagcaattga tttgttcata tttaatttca
    13681 ttttttccat aaatgggtat tagatataaa cagcaaaata tttccgatac gtgtcattct
    13741 tgaatttcca atcatctaaa acaagtaaag gataatcaat cccctgtata tcaaggaatt
    13801 ggctaccctt tttacttttt tacacatcct gtttgataga ctcattttaa catcacgagc
    13861 atactccaat ggaaatcgct aggcaagaga taaactttca gatatccgca gagagatcat
    13921 cgcctctttt tgtcgcaagc attctcctct cctagtcatt ttctacctta tcctctacct
    13981 gaggatagag agttgttccc caaatagaaa tcgtccgctt acgcactagt ggcaaatcgg
    14041 ttttttcata aaccgtacgc caccattccc aggcaagccc ggtacactct ctaattttga
    14101 cagagagatt acgaacattc ccttttaaag gaatactagt ggtaaagtga gccgtcaaat
    14161 cctgcccatt tctgtcccaa gccttaggag tcaagacttc cttaccttga tgatcatagg
    14221 ataattcatc ccaagtaata taatattggg caacataggc accactatga tccagcagta
    14281 aatctccgtt tctgtaagct gtaaccttag tctcaacata gtctgtactg ttttgaaagg
    14341 tcgcaactac attgtcacgt aaaaaagaag ttgtatagga aatcggcaag cctggatgat
    14401 ctgctgtaaa gcgactgcct tcttgaatca agtcctctac catatccacc ttgcctgtta
    14461 caactcgggc acccgaactt gggtcgcccc ctaaaataac cgccttcact tctgtattgt
    14521 ccaaaatctg cttccactct gtctgaggag ctaccttgac tccttttatc aaagcttcaa
    14581 aagcagcctc tacttcatca ctcttactcg tggtttccaa cttgagatag acttggcgcc
    14641 cataagcaac actcgaaata tagaccaaag gacgctctgc agaaattcct ctctgtttta
    14701 aatcctctac cgttacagta tcttgaaaca catctcctgg atttttaaca gcgtctacgc
    14761 tgactgtata ataaatctgc ttaaaattaa caatctgaat ctgcttttca cctgaatgga
    14821 cagagttaaa atcaatatca agagaattcc ctgtcttttc aaagtcagaa ccaaacttga
    14881 ccttgagttg ttccatgctg tgagccgtta ttttttcata ctgcattcta gctgggacat
    14941 tattgacctg accataatct tgatgccact tagccaacaa atcgtttacc gctccgcgaa
    15001 cacttgaatt gctggggtct tccacttgga gaaagctatc gctacttgcc aaaccaggca
    15061 aatcaatact ataagtcatc ggagcacgat caaccgcaag aagagtggga ttattctcta
    15121 acaaggtctc atccactacg agaagtgctc caggatagag gcgactgtcg ttggtagctg
    15181 ttacagaaat atcacttgta tttgtcgaca agctccgctt ctttctttcg ataacaacaa
    15241 actcatcggg tagctgatta ccctctttga tgaaacgatt ttcaatactt tctccctgat
    15301 gggtcaagag tttcttttta tcgtaattca tagctagtat aaagtcattt actgctttat
    15361 ttgccatctt ctacctccta ataagttcct ggattgagtt gcataaactc agacttgttc
    15421 agcgaaatca gccgtggttg gactaagtga tccaaaattt cctcgtacaa ttcttctgag
    15481 acattgcgtc gccgtctggc tagataagaa gtcggaataa ccgtattatc caacataaat
    15541 accttatcta agtcaatcaa ggttggtctt gtaaaaggat tacgagctag atccggctct
    15601 tctatcataa agttcttgac caaacgtctg gtcaagagag ctggtttgaa ggtctgattt
    15661 ttaaccaact ctttgttttt agtcatgctg ttgtcaatac agatatacat atgattcttc
    15721 acagccaaat cgctactaat agtcggaaaa ggcaaataaa gagctacaac atctcctctc
    15781 ttaatcaagc aagagcaccc ccttttctcc taatgtaaca tagacaggat tgaccaagtc
    15841 ttctgattga ctcagaattt ccaaagtttg agtttggcgc gctgtcaatt tagtagcatc
    15901 ttgtctcttc aatacaaaat gcttgtcgcc aataaccttg acaatataat ccttctccaa
    15961 agctgactgg taaatccaca tcagatgttg tctgtcctga gaactcaaga gagaaggatt
    16021 ttcaagcctc ccgatagtct gataaaaatc aaaaacagga gctaactcct gccaatctga
    16081 ttggctagtt gtcaaggcta gaaaaagggc tttgcgagct gatacttctt ggttagcctt
    16141 gagagttact ttcccctcca agttttttag aaatcgggaa actccagaaa gcaaattttt
    16201 ctctaactgc gagaaataaa aacctttcgt tcccagacat aagtctttca tgtcgctttc
    16261 tctagcaaat aagagctcaa acatttgata gtaaaagaaa aatatctggc actgggtcgc
    16321 gctcatcttt tccttatcgg cttctttttt taaccagagc aagggcgaca ggtaactgga
    16381 ttgagacatt tcctctacct cctactcttt tttaactgga gcatctgcac tagctgccac
    16441 ttcttttgac tggatacttt cccactggtt aatctcctct gagataagac cttcgcatgt
    16501 cttgacaaat agggcaaaag ccttggtctt tcctgcatat ttctccgttt ggcattgata
    16561 gaggaatttt tctttctcca ggagttgcgc agttttttgg taagaaatcc aattttcctt
    16621 tgcattatac aaattgataa tcccctcaca cagcaagccg agactggata aggcaaccga
    16681 aatcaaacgg tagcgatcac ctggcatagg aatagcacaa aagacagcta tgaggaaacc
    16741 tgccacgatt tctgttattt ttaatacctt atagcgccta cgatgttgaa cgcttttctt
    16801 taaaaaatga gctatctgta cgtctaatcg ctctgtcagg tacatttctt ctggcgtcat
    16861 attcgtaact cctttcattt actttgataa ccatagtata acatatttga atatcaaagt
    16921 atagtaaaaa ctggactata acttattaga ctccctgcga aacaaaatat ggtatagtag
    16981 ttctatgaat gatgaagcaa gtaaacaatt aactgataca cgatttaagc gtcttgttgg
    17041 tgttcagcgt accacttttg aagagatgtt agctgtatta aaaacagctt atcaaaaaag
    17101 tcggacaagc tggtaaaatc ttggctgaca gtaattatca agggctcatg aagatatatc
    17161 ctcaagcaca aactccacgt aaatccagca aactcaagcc actaacagtt gaagataaag
    17221 tctataacca tgcgctatct aaggagagaa gcaaggttga gaacatcttt gccaaagtaa
    17281 aaacgtttaa aatgttttca acaacctgtc gaaatcatcg taaacgcttc ggattacgaa
    17341 tgaatttgag tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta
    17401 ttatatatat gcacagacat atgaaatctt tttgatacag ccttcttttt acatagagag
    17461 acagcagtta tgatatattt atccattcac aacatcttct gtaccaatac tacaataatt
    17521 ggtacatttt gattacacaa tcgtttcaaa aaatatgatg gaagactgag tattttatag
    17581 taattccctt cctatcaact agatcaacca gttgagtgtt gaatgttgaa acaccctagc
    17641 ctcagcaaag gactatgatc agcatttcct agccaatagg acgctccaaa tcacctattc
    17701 taagtccatc actagcttgg tcagtaagat acagactgca aggcaagact gacgtggttg
    17761 atgagatttt ggaagagtat tacaatcata gattcaccag tagacttgac cgcagattta
    17821 actatagtag attgaaacta gaatagtaca cttctgcttc taaaacattg ttagaaatcg
    17881 atttgactgt cctgatcaat ttgtcctgtt ctcgtttcat tttactatac taaaaaacca
    17941 cctgaaacaa atcaagtgac tagaggataa aaacaaagac tgggcaaaaa gttttgaaaa
    18001 gcaaaaaacg cataatatca ggtcttgaaa aaccttgata ctatacgttt gattgtggga
    18061 agattgactt cattttctcc tgaaattgaa gttttttcta gtttccaggt atagacgaat
    18121 tcaatatagc catctcgcta caattatttt aaccatcgaa aaaatttttc agaatttcaa
    18181 aaatttcatt ttgatccaat gaaatgtcat tattttgaag aatagttgac ttcacaggct
    18241 ctaattctat cacaaagcgg tgcatgtctg cttttaccgc actacttgat gttatagccg
    18301 ataacttttc atctcctagc aaggaagctg tcaaacgggt aaggtcattc aaatgctttt
    18361 taatggactt acttaaacct tgagcacctg tggcagagcg ctccctcata tccagccaag
    18421 ctttagcctt aaaaataatt aatactcaat gaaaatcaaa gagcaaacta ggaagctagt
    18481 tgcaggctgc tcaaaacact gttttgaggt tgtagatgga agctgacgtg gtttgaagag
    18541 atttt
//
LOCUS       DAVLVZ010000035        18266 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000035 DAVLVZ010000000
VERSION     DAVLVZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18266
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.035"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            140..436
                     /locus_tag="VYN77_001269"
     CDS             140..436
                     /locus_tag="VYN77_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1558726.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIDSNGVSIE"
     gene            557..1501
                     /gene="manA"
                     /locus_tag="VYN77_001270"
     CDS             557..1501
                     /gene="manA"
                     /locus_tag="VYN77_001270"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1558727.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(1604..2989)
                     /locus_tag="VYN77_001271"
     CDS             complement(1604..2989)
                     /locus_tag="VYN77_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV1558728.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQATFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIERSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALRQSFFALSLGVTAMLT
                     YASYLDKKTNLVQSRISIVAMNISVSIMAGLAIFQARSPFNIQSEGGPSLVFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVDNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKAFFDAMDFLVSNLLMPFGALFLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQV"
     gene            3072..3817
                     /locus_tag="VYN77_001272"
                     /pseudo
     CDS             3072..3817
                     /locus_tag="VYN77_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3846..4301)
                     /locus_tag="VYN77_001273"
     CDS             complement(3846..4301)
                     /locus_tag="VYN77_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1558729.1"
                     /translation="MNRREAVEFVNMCMIKNGDKILVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4321..5496)
                     /locus_tag="VYN77_001274"
     CDS             complement(4321..5496)
                     /locus_tag="VYN77_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1558730.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5555..6400)
                     /locus_tag="VYN77_001275"
     CDS             complement(5555..6400)
                     /locus_tag="VYN77_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1558731.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6525..7082
                     /locus_tag="VYN77_001276"
     CDS             6525..7082
                     /locus_tag="VYN77_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1558732.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7101..7568
                     /locus_tag="VYN77_001277"
     CDS             7101..7568
                     /locus_tag="VYN77_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1558733.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7657..8286
                     /gene="upp"
                     /locus_tag="VYN77_001278"
     CDS             7657..8286
                     /gene="upp"
                     /locus_tag="VYN77_001278"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1558734.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8460..9050
                     /gene="clpP"
                     /locus_tag="VYN77_001279"
     CDS             8460..9050
                     /gene="clpP"
                     /locus_tag="VYN77_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1558735.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9129..9377
                     /locus_tag="VYN77_001280"
     CDS             9129..9377
                     /locus_tag="VYN77_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1558736.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9478..10638
                     /locus_tag="VYN77_001281"
     CDS             9478..10638
                     /locus_tag="VYN77_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1558737.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10906..11775
                     /locus_tag="VYN77_001282"
     CDS             10906..11775
                     /locus_tag="VYN77_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1558738.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11779..12735
                     /locus_tag="VYN77_001283"
     CDS             11779..12735
                     /locus_tag="VYN77_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1558739.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12735..13499
                     /locus_tag="VYN77_001284"
     CDS             12735..13499
                     /locus_tag="VYN77_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558740.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13499..14209
                     /locus_tag="VYN77_001285"
     CDS             13499..14209
                     /locus_tag="VYN77_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558741.1"
                     /translation="MSVLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14623..15279
                     /locus_tag="VYN77_001286"
     CDS             14623..15279
                     /locus_tag="VYN77_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1558742.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIVRTSAKVL
                     "
     gene            15387..16482
                     /gene="prfB"
                     /locus_tag="VYN77_001287"
     CDS             join(15387..15458,15460..16482)
                     /gene="prfB"
                     /locus_tag="VYN77_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1558743.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKMSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTYIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16500..17192
                     /gene="ftsE"
                     /locus_tag="VYN77_001288"
     CDS             16500..17192
                     /gene="ftsE"
                     /locus_tag="VYN77_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1558744.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17185..18111
                     /locus_tag="VYN77_001289"
     CDS             17185..18111
                     /locus_tag="VYN77_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1558745.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANAPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         5614 a         3425 c         3978 g         5249 t
ORIGIN      
        1 tttatgatat ttagctgtac gagagtcttt aaaaagtgtt ttgatggttt ggatttcttc
       61 tttagttgat ttcatatcac tattatataa tgctttttta ttttagtata gtattatcgt
      121 agaaaatgga ctcggtgcta tggatacacc ggatgagaat ggttatgtag cagatgacta
      181 tcggattact tacttagagg cccacatcaa ggccatgcga gatgccattt acaaagacgg
      241 ggttgacttg cttggttata cgacatgggg ctgtatcgat tcggtttcag ctggcacagg
      301 ggaaatgaat aagcgctatg gctttatcta tgtagatcga gataatgtag gaaacggagc
      361 tctcaaacgt agcaagaaga agtccttcta ttggtacaag gatgtcattg atagcaatgg
      421 tgtaagcatc gagtagagca catttgttta ctatttttat aaaatcttct ccctgcttta
      481 taagatgtaa aaaagagttc aaatggaact ctttttttgc tataatgagg ggagaaaaat
      541 cagacaggag attaagatgt cagaaccatt atttttacaa tcagttatgc aagaaaaaat
      601 ctggggtggt accaagctac gtgatgagtt tggctacgac atcccaagtg aaaaagtcgg
      661 agagtactgg gctatctcag ctcatctaaa tggtgtttct aaagttgcca atggtcgtta
      721 cgagggaaca gatcttgcta ctttgtatgc ggaacaccgt gaattatttg gcaatcgtcc
      781 agaacctgta tttccacttt tgaccaagat cctcgatgcc aacgactggc tcagtgtcca
      841 agttcaccca gacgatgctt atggactcga gcatgaaggc gaacttggaa aaacagaatg
      901 ttggtacatc attgcagcag atgaaggttc agagattatc tacggtcaca atgccaagtc
      961 aaaagaagaa ctccgccagc aaatcgagga taagaactgg gatgcgctgt tgacaaaagt
     1021 gccagttaaa gcaggagatt tcttctatgt accaagcggt accatgcacg ctatcggggc
     1081 gggtatcttg attcttgaaa cccagcagtc tagcgatacc acttaccgtg tctatgactt
     1141 tgaccgtaag gatgacaagg gcaatttacg tgaacttcac cttgaaaaat ctatcgatgt
     1201 cttgaacatt ggtgaacccg caaatagccg tccagtgaca atcaaagcag atgatctacg
     1261 ttccactctc cttgtatcca atgatttctt cgcagtttac aaatgggaaa ttactggaaa
     1321 agttaacttt gaaaagacag ctgactacag cttgttgagt gtcttggctg gtcaaggtca
     1381 actaactgtt gacgggaaaa actatccaat ccaaaaaggg agacacttta tcctaccaag
     1441 tgatgttgaa tcttggactc tagaagggca agatttagaa ttaattgtta gtcatccata
     1501 aaaaagaaag gacctgagtt cattactcaa gtccttttga ttacataaat tgggcgatga
     1561 agaccacgat gatgattgga atgacgaaac gaagaaggaa gagtcagact tggaacagtc
     1621 cttgtttcca tgctctttca tcgagatgga gttcctccat tgcaagagcc tttttaaaga
     1681 tatagcctgt aaaaagtgaa aggaagagag ctccaaatgg catgaggaga ttggaaacca
     1741 agaagtccat agcgtcaaag aaggctttac caaaaatgtg aacatccgcc atgacaccgt
     1801 aagatagggc tgaaggaatg ccaaagacaa aggtcaaaat tcctaaaata acactccatt
     1861 tagcacgctt gctgttgtcc tgattggtga tattgtctac attgatttcc agcataacga
     1921 cagaagaagt gacagtcgca aagaggaaga ggacatagaa aatggttcca aaaggcatct
     1981 tgtcaaagag ttgaggcaag acgataaaga ccaagctcgg tcccccttca gactggatat
     2041 tgaaggggga ccgagcttgg aaaatggcta gacctgccat gatggatacc gagatattca
     2101 tggctacgat ggagattctt gactggacca gattggtttt cttgtccaag taagaagcat
     2161 aggtcaacat ggctgtaacc cctagtgaga gggcaaagaa agattgtctc agagcataga
     2221 ggagaccagc gctagtcagt tttgaaaaat ctggtttgag gaagtaaaga accccttcca
     2281 tggcatttgg caaactgaga gagcgttcga tgatgacgac aaagatgata aagagcaggg
     2341 gcatcatgac tttcgaagct ctttcaatcc ctttttgaac cccacgtgat acaatgaaga
     2401 tattcaatag gataaaggtc gcttgagctc ctagagcaat ggctggattt gaaatgattg
     2461 aagtaaataa ctgagcataa tcacccgttc caccaagttg gaacaatttc ccaaactcaa
     2521 tacctagata gactagaatc catcctccga taacactgta aaaagataag aggataaaga
     2581 gggcaaaggc gccaatccaa ccgataaagt tgtacttgtt attcttgccc agttttccaa
     2641 aggttttgat agcggaaacg ccagcactac ggccaagggc aaactcagcc agcaggagag
     2701 ggaaaccgat taaaatagtg gaaatgagaa agattagtaa aaagcctcca ccgccattag
     2761 cagcagtcat gtagggaaac ttccaaacgg aaccaagccc gatgaccgag ccagcagatg
     2821 ctagaataaa gccaagtttc gacccccatt gtgatttttc tgacatagtt aaactctgat
     2881 ctcctttttc aaaataagaa aaagctactt gagttgtcaa atagccttct gtttgattga
     2941 tagacttgac tatcctatta tgttttctaa ggtctgtcaa gaaaaccatt ttcaagtttt
     3001 cacacctttc tcaaaaacgc ttgactctga cctaagggga ggggttatac tatcattgta
     3061 aggaggaaat catgtaccat ataaaagaag ctgcgcagct ttcaggtgtc tctgtcaaga
     3121 ccctgccctg catcactatg acaagatagg actcttggtc cccttaaagt cggaaaacgg
     3181 ctatcgaacc tatagtcaag aggatttgga acgccttcag gtcattcttt actacaaata
     3241 tctaggcttt tctttagaga aaatagcaga gctgttaaag gaagaaagga cagatttatt
     3301 gccccattta actaggcagt tggactatct aactcgcgaa aggcaacatc tggatacctt
     3361 gatttccacc ttgcaaaaaa ctattcaaga acaaaaagga gaaagaaaaa tgaccattga
     3421 ggaaaaattc acgggattta gctatcaaga caatcaaaaa taccaccaag aagcggtaga
     3481 taaatatggt caagaagtca tgggacaagc gctcgaacgc caaaaaggtc acgaagacga
     3541 ggctacggcc gcctttaacc aagtctttca aactttggca caaaatcttc aagttggttt
     3601 acctgcaaca gcaaccgaaa accaagagca agcagccaag ctcttgcaag ctattcgcac
     3661 ttatggattt gactgctcta ttgaggtatt cggtcatatc ggtaaaggtt acgtctacaa
     3721 cccagagttt aaggaaaata ttgacaagtt tggttctgaa acagcccagt acacgtcaga
     3781 tgccattgcg gcttacgttc agacaaatgc agaataaata ggctaggaat ttcctagcct
     3841 attttttatt tcaaatcata aagccagtcg tcaccgtttt tgtagtaaaa gaattcactg
     3901 agatcttctt ctagaaacac acgaagcata tcagacatat catcggttgc aagttttaga
     3961 tgagaaagat tttcaaagtc ctcccaccaa actttccctt cgtctgaaga ctggagttca
     4021 ccagtaaagt gttctgtctt gtaaaaaagg acgacataac gataatcctt gtcgtcatac
     4081 cagtttttga taccacagag ttggggtttg gaaatgatca gaccagtttc ttctttcact
     4141 tcacgaatga cagcatcgac aaaggattcg ccacgttcaa catgaccacc aggaaaagta
     4201 atgccaggcc agtcgggact aactcggtct tggaccagga tcttatctcc gtttttaatc
     4261 atacacatgt taacaaattc gactgcctct cttctgttca ttcttcacaa cctttaatct
     4321 ttaatcataa tgcagacttc ccgccaccca gccggtacag agggcagaag tgatgttaaa
     4381 gccacccgtg tgggcattga tatccataac ttcgcctgca aagtggaggc caggtaccag
     4441 cttactttca agggttttag gattgatttc cttgagactg actccaccct tggtaacaaa
     4501 ggactttgca agggacattt ttccagttac aggaatttta agttctttaa tggactggac
     4561 aagttgttct cgttcctttt cagtcagctg tttgactttt tcaggatatc cttgtacaaa
     4621 aaattcggcc aagcgttctg gtaacaaggt ttttaaagcg tttttcaagg atttttcccg
     4681 attttcttct agaaatgtaa ccaagtcctt ctcagaaagt tgaggcaaaa catcgagtga
     4741 gagaacctcc ccacctttga caaagctaga catgcgtagg gcagcaggac ctgacaaacc
     4801 aaagtgggta aagagtaaat catgagtgat gacatgctta ccataactta gggtcacatc
     4861 gtccagagaa atcccttgta aggctttatg tggaaaatct gttaataaag gactttcagc
     4921 agcctcaaga tcggtgatgg tatgcttaaa atggcgagca atctcgtgac caaaaccagt
     4981 cgaaccagtc gaaggataag acttaccacc tgttgtgaca atgagtttct cacaagtgaa
     5041 ggtttgatcc gctgacttaa ggacaaactg gtcatctact tttttaacag aaacgatttc
     5101 tatttgagta gcaacttgac cacctagttc agtgattttc ttttccaaag cttcgataat
     5161 agtccgagac ttgtcactgg ctggaaagac gcgtccgtgg tcttcgacct taagtttaac
     5221 accattttct gtaaaaaagt tgatgatgtc atgattatcg aactgggaga aaacactgta
     5281 aagaaagcgt ccgtttccag gaattccagc tagcaggttg tctaagctac cattgttggt
     5341 cacattgcaa cgtcccccac cagtcccagc taattttttt ccaagtttcc gatttttttc
     5401 gatgaggagg gttttctgtc cataaaagct actggaaatc gtagccatca taccagcagg
     5461 tcccccaccg atgacaatag tatcaaaatg tttcatagct ctattgtacc acaaaaaaac
     5521 aagagatgat ggtcacctct tgtcaagaat gcaattaatc aatttcatag cccatcagca
     5581 aaccgccctc ttctgcatag aaactgcaga gaccagaggt tggtagaatt ttaatatccg
     5641 cttgtgggaa ggtttcacgg attcgctctg agagctgttg acaacatttt tcgttattgc
     5701 gttgggccat gacaatacgg ccaccagcat atccagcttt tactaactca tcataggcag
     5761 cttgaactga tttctttgat ccccttgctt tttgtagcaa ttcgagagtc ccagtttcac
     5821 tagcttttcc gaccatacga atgttgagaa ggccaacgac cgtaccgata agcttgctca
     5881 aacggccgtt cttcaccaag ttatcgactt tggctaggac aaagagcaac ttagtttttt
     5941 cttgataggc ggtgatagct tcaaccactt cttcaaaaga caagccctgg tcaatcaagt
     6001 cattcaattt ttctacgagt aggtcaactt caccaccagc agataaacta tcaatcacat
     6061 gaatcttagt gtcaggatgg tcttccagat aaatattctt tgctagttga gcactattgt
     6121 gactgccaga aagggtacct gtgatggtta ctaggaaaat gtttttggca ccttcaaatg
     6181 ctcgcaaata gtcatctggg cttggacaag ccgattttga agcttctgca gttgcataca
     6241 tggtttccat catttggtca atatcgagac tggcgtcatc aacaaagacc tgatcagcta
     6301 cttgaatggt taaggggaca cttacaaagg ttgtgttaat agctggtgtt ggcagttgac
     6361 gataatcaca accagagtca gcaataatct tccaagtcat agaaattctc catctttgtc
     6421 agttatacat tgacaaaggt tctgtctttt tttacaatta tatcatgaaa acccttgaaa
     6481 caaaagcatc atccgtctgt tgtgacaagt agaaagaaaa tgttatgtct gaacgtagaa
     6541 tctctgaaaa gtctcttgaa aatctcagaa aatcaaacca agaatccaat ttattaacca
     6601 gagaagccat tgaaacagcc ctcttgcaac tcttggaaaa aaaggaactg accaagatta
     6661 gtatttctga attggtcaaa cgtgcaggtg tttcgcgtgc ggccttttat cgcaattatg
     6721 actccaaaga ggaaatttta gagagcgtct ttaaacgaac tgtccacaat attatggaac
     6781 agctgcatca ttacgattta aagacagacc tttatttggt ttgggttcac cttttccggg
     6841 aggccagaaa ggaagctaga gtaattcaat tggccttgga ttaccatctg gaaaaaatct
     6901 ttgtccaagc catgcaggaa tttctagaaa aataccatgg gaaatcaaaa ggtgtcagct
     6961 cttatcttca ttccttctgg agctcagcca tcgtctctgt ccttctaaaa tggatcaagg
     7021 atggcatgaa ggtaccagct gaaaagattg cagatttagg tttaccattt tttaaaaaat
     7081 agagaaaaag gagaaaagag atgactgaaa aaagactggc ctgggatgag tattttgcag
     7141 cccaagctct actaattgcg aatcgttcca cttgtaaacg tgctaaagtg ggcgcgattc
     7201 tggtaaaaga taataaggtt atttccactg gttacaatgg ttcggtgtca gggactgagc
     7261 attgtattga tcacgaatgt ttggtcattg aaggccactg cgttcgcacc cttcacgctg
     7321 aggtcaatgc tatccttcaa ggtgctgagc gtggtgttcc caaaggcttt acagcctatg
     7381 taacccattt tccttgtctc aactgtacaa aacaattgct tcaggtcggc tgcaagcgcg
     7441 tggtttatat caaccagtac cgaatggacg actacgccca atacctttat caagaaaaag
     7501 ggacagaatt gacccattta ccacttgaga cagtacagac agctcttaaa gaggctgatc
     7561 taatgtaaaa attatcaaaa ataaatggtt tagaaagatt tttaaaccgt tttttggtat
     7621 aataagaaga ataaattgaa agaaggaatt ccaaaaatgg gaaaaattga agttattaat
     7681 cacccactga ttcaacacaa attgtcaatc ttgcgtcgta cagatacttc tacaaaagct
     7741 tttcgtgagc tagtagatga gattgcaatg ttgatggggt atgaagtact tcgtgatctt
     7801 ccactagaag atgtggaaat cgaaacacca attacaaaaa cagttcaaaa acaattggca
     7861 ggtaagaaat tggccatcgt cccaatcttg cgtgcaggta tcgggatggt tgatggtctc
     7921 ttgagcttgg ttccagctgc taaagttggc cacatcggta tgtaccgtga tgaagaaaca
     7981 cttcaaccag ttgaatactt ggtgaaattg cctgaggaca ttgaccaacg tcaaattttt
     8041 gtagtagacc caatgttggc aacaggtggc tcagcaatct tggctgttga ttctcttaaa
     8101 aaacgtggcg catcaaatat caaatttgtc tgccttgtat ctgctccaga gggtgtaaaa
     8161 gcccttcaag aagctcatcc agatgtagaa atctttacag cagccttgga tgaacgtttg
     8221 aacgaacacg gttatatcgt tccaggtctt ggagatgctg gagaccgctt gttcggtaca
     8281 aaataagatc gaaaagaaga atgacttgga aaagtatttc cagtcacgaa aggaggttgg
     8341 gtttttgttt ctgtctaatg aaagcagagc aaaaatttga ccttttttga ccaagatatt
     8401 ataatagtct tatcttcagt catttgacca acaaaattaa aactcaaaag gagaaatgaa
     8461 tgattcctgt agttattgaa caaacaagcc gtggagaacg ttcttacgat atttactcac
     8521 gtcttctcaa agaccgcatc attatgctga caggtccggt tgaagacaat atggctaact
     8581 ctgttattgc ccaactgctt ttcttggatg cccaagatag tacaaaagat atttaccttt
     8641 atgtcaatac accaggtggt tctgtttcag ctggtttggc aatcgtagat accatgaact
     8701 ttatcaaggc agatgtccaa accattgtta tgggaatggc tgcatctatg ggaacagtca
     8761 tcgcatcaag tggagcaaaa ggcaaacgtt tcatgcttcc aaatgctgaa tacatgattc
     8821 accaaccaat gggcggtaca ggtggtggta cccaacaaac tgatatggct atcgctgcag
     8881 aacacttgct caaaactcgt aataccttgg aaaaaatctt ggctgaaaat tcaggtcagt
     8941 caatggaaaa agtccatgca gatgcagaac gtgataactg gatgagcgcc caggaaacac
     9001 ttgaatatgg ctttattgat gaaattatgg ccaacaattc attgaactaa tgatgataga
     9061 aggcaaactc gactgggttt gctttttttg gtataatagg gagagatttc ttagaaagag
     9121 gatttatcat gtttgaaaaa gtcaatcgct ctggcttgat tatctatctt tactataatc
     9181 gtgatgccaa aaaactgcag gattatggag atattaccta tcattccaag aaacatcgtt
     9241 acttacaact ctatgttcca actcaagaag tggagcaatt ggtcggacgc ttgagcaagg
     9301 aaaaatttat taaaaaagtt agggtttgtc atatccaaga gttggaaaca ccctttgtgg
     9361 gcaatcttta tcgataggaa aacgttatca tcgaaaaagt tcaaggaaaa cattgacaat
     9421 tttctgataa ttcggtatat tcttaacaga ctatttaaga aataaggaga caaaaagatg
     9481 aagaaaaaat ttgccctatc gtttgtggcg cttgcaagtg tagcacttct tgcagcctgt
     9541 ggagaagtga agtctggagc agtcaacact gctggtaact cagtagagga aaagacaatt
     9601 aaaatcgggt ttaactttga agaatcaggt tctttagctg catacggaac agctgaacaa
     9661 aaaggtgccc aattggctgt tgatgaaatc aatgccgcag gtggtatcga tggaaaacaa
     9721 atcgaagtaa tcgataaaga taataagtct gaaacagctg aggctgcttc agttacaact
     9781 aaccttgtaa cccaatctaa agtatcagca gtcgtaggac ctgcgacatc tggtgcgact
     9841 gcagctgcgg tagcgaacgc tacaaaagca ggtgttccat tgatctcacc aagtgcgact
     9901 caagatggat tgactaaagg tcaagattac ctctttattg gaactttcca agatagcttc
     9961 caaggaaaaa ttatctcaaa ctatgtttct gaaaaattaa atgctaagaa agttgttctt
    10021 tacactgaca atgccagtga ctatgctaaa gggattgcca aatctttccg cgagtcatac
    10081 aagggtgaaa tcgttgcaga tgaaactttc gtagcaggtg acacagactt ccaagcagcc
    10141 cttacaaaaa tgaaagggaa agactttgat gctatcgttg ttcctggtta ctataatgag
    10201 gctggtaaga ttgtaaacca agcgcgtggc atgggaattg acaaaccaat cgttggtggt
    10261 gatggattca acggtgagga gtttgtacaa caagcaactg ctgaaaaagc atcaaacatc
    10321 tactttatct caggcttttc aactactgta gaagtttcag ctaaagctaa agccttcctt
    10381 gacgcttacc gtgctaagta caatgaagag ccttcaacat ttgcagcctt ggcttatgat
    10441 tcagttcacc ttgtagcaaa cgcagcaaaa ggtgctaaaa attcaggtga aatcaagaat
    10501 aaccttgcta aaacaaaaga ttttgaaggt gtaactggtc aaacaagctt cgatgcagac
    10561 cacaacacag tcaaaactgc ttacatgatg accatgaaca atggtaaagt tgaagcagca
    10621 gaagttgtaa aaccataata gaaaaatgtt gaaataggga atgagccttt gactcactcc
    10681 ctgtttcgat atttaatact cttcgaaaat ctcttcaaac tgcgtcaacg tcgccttgga
    10741 ttatatatgt gactgacttc gtcagtctta tctacaacct caaagcagtg ctttgagcaa
    10801 cctgcggcta gtttcctagt ttgctctttg attttcattg agtataagaa cctatcaaaa
    10861 agtgagggaa aaccctcgga attataaata gaaagagtga atcttatgct ccaacaactc
    10921 gtaaatggtt tgattctagg tagtgtttac gcgctgttag ccctaggata taccatggtt
    10981 tacggaatta tcaagctcat caacttcgcc catggtgata tttatatgat gggagccttt
    11041 atcggttatt tcttgatcaa ttctttccaa atgaatttct ttgtagcgct tattgtagct
    11101 atgctagcga cagctattct tggtgtcgtg attgagtttc ttgcttaccg acctttgcgc
    11161 cactctactc gtattgctgt tttgattacg gctattgggg tttctttcct attggagtat
    11221 ggaatggtct atctggttgg tgccaatacc cgtgccttcc ctcaagcgat tcaaacagtt
    11281 cgatatgatt tgggaccaat tagcttaaca aatgtgcagt taatgatttt gggcatttcc
    11341 ttgattttga tgattttgtt acaagtcatt gtccaaaaga ctaagatggg gaaagccatg
    11401 cgtgcagtat cagtagatag cgacgcggcg caattgatgg ggatcaatgt aaaccgtacg
    11461 attagcttta ccttcgcttt gggttctgct cttgcgggtg cggctggtgt tctgattgct
    11521 ctttattata actctcttga gcctttgatg ggggttactc caggtcttaa atctttcgtt
    11581 gccgcagtac ttggtggtat cggaattatt cctggtgcgg ctcttggtgg ctttgtgatt
    11641 ggtctattgg aaacctttgc gacagccttt gggatgtcag atttccgtga tgccattgtt
    11701 tatggaatct tgttgttgat cttgattgtc cgcccagctg gtatccttgg taagaatgtg
    11761 aaagagaagg tgtaaacgat gaaggaaaat ttaaaagtta atattctatg gttactcctt
    11821 ttgttagctg gctatagctt gattagtgta ctggtttcag tcggagtact taatctattc
    11881 tatgtacaga ttttacaaca aattggaatt aatattattt tggctgttgg tctcaactta
    11941 atcgttggtt tttcaggaca attttcactt ggtcatgctg gtttcatggc gattggtgcc
    12001 tatgcagcag ctattattgg ttctaaatca ccaacctacg gtgccttctt tggagctatg
    12061 cttgtagggg ctttgctttc aggagcagtt gccttacttg tcggcattcc aaccttgcgc
    12121 ttgaaggggg actatcttgc ggtagcaact ctgggtgttt ctgaaattat ccgtatcttt
    12181 atcatcaatg gtggaagtct tacaaatggt gcggcaggta tcttagggat tcctaacttt
    12241 acaacttggc aaatggttta cttctttgtt gtgattacaa ccattgcaac cttgaacttc
    12301 ttgcgtagcc caattggccg ttcaaccctc tctgttcgtg aagatgaaat cgctgctgag
    12361 tcagttgggg ttaatacgac taaaattaaa atcatcgctt ttgtctttgg tgccattact
    12421 gcaagtattg ctgggtcact tcaggcagga tttatcgggt ctgttgtacc gaaagattac
    12481 accttcatca actcaatcaa cgttttgatt attgttgtat ttggtggact cggttccatt
    12541 acaggtgcga ttgtttcggc tattgttctg ggaattttga atatgcttct ccaagatgtt
    12601 gctagtgtgc gtatgattat ttacgctttg gccttggtat tggtaatgat tttcagacca
    12661 ggtggactcc ttggaacgtg ggaactgagc ctatcacgtt tctttaaaaa atctaagaag
    12721 gaggaacaaa actaatggca ttacttgaag taaaacagtt aaccaaacat tttggtggtc
    12781 taacagctgt tggagatgtg actcttgaat tgaacgaagg ggaactggtt ggattaatcg
    12841 gtccaaacgg agctgggaaa accacccttt tcaacctttt gaccggtgtt tatgaaccaa
    12901 gcgagggaac agtaacccta gatggtcacc ttttgaatgg gaaatcacct tataagattg
    12961 cctctttggg acttggacgt actttccaaa atatccgtct ctttaaagat ttaacagttt
    13021 tagacaatgt tttgattgct tttggaaacc atcacaaaca acatgttttt actagtttct
    13081 tacgcttacc agctttttac aagagtgaaa aagaattaaa ggctaaagct ttggaattgt
    13141 tgaaaatctt tgatttagat ggtgatgcag agactcttgc taaaaatctt tcctacggac
    13201 aacaacgtcg tttggaaatt gttcgtgccc ttgctacgga acctaaaatt ctcttcttag
    13261 atgaaccagc agcaggtatg aacccacagg aaacagccga attgactgag ttaattcgtc
    13321 gtatcaaaga tgaatttaag attacaatca tgttgattga acacgatatg aatctggtca
    13381 tggaagtaac agaacgtatc tacgtacttg aatatggccg tttaattgct caaggaactc
    13441 cagacgaaat taagaccaat aaacgcgtta tcgaagctta tctaggaggt gaagcctaat
    13501 gtctgtgtta aaagttgaaa atctttctgt gcattacggt atgatccaag cagtccgtga
    13561 tgtaagcttt gaagttaatg aaggagaagt tgtttccctt atcggtgcca acggtgcagg
    13621 taagacaact attcttcgca ccttgtcagg tttggttcga ccaagttcag gaaagattga
    13681 atttttaggt caagaaatcc aaaaaatgcc agctcagaaa attgtggcag gtggtctttc
    13741 acaagttcca gaaggacgcc acgtctttcc tggcttgact gttatggaaa atcttgaaat
    13801 gggagctttc ttaaagaaaa atcgtgaaga aaatcaagct aacttgaaga aggttttctc
    13861 acgctttcct cgtcttgaag aacgtaagaa ccaagatgca gctactcttt caggagggga
    13921 acaacaaatg cttgccatgg gacgcgctct tatgtcaaca ccaaaacttc ttcttttaga
    13981 tgaaccatca atgggacttg ccccaatctt catccaagag atttttgata tcattcaaga
    14041 tattcagaag caaggaacaa ccgtcctctt gattgaacaa aatgccaata aagcacttgc
    14101 aatctctgac cgaggatatg tactggaaac agggaaaatc gtcctatcag gaacaggaaa
    14161 agaactcgcc tcatcagaag aagtcagaaa agcatatcta ggtggctaaa acaatccagt
    14221 ggattgtttt agtcggcaga tgaagattac gaagtaatca tcattatagt ctgggagact
    14281 agtttaggtt gcagataaag attgcgaagc aatcatcaaa tccagaggat tgttttagcc
    14341 ggcagatgga gattacgaag taatcatcaa tatagtcctg gggacctttt tagtcggtgg
    14401 atagagattg caaacaaata tgcatctaca ttgaaagctt aatttctaat aattgaaaaa
    14461 atcgaatgaa aaatttctta ccttcattca cagagctcta tttcagagct ctttttgcta
    14521 gcttattcat acttttttga atttcgaaaa aggaatgtaa gcgtttgata gatttacaaa
    14581 aagattgtat aatggggata agaatagaaa aggagaagtc tcatggcagt taaagatttt
    14641 atgacccgca aggtagttta tattagtcca gatacaacag tatctcatgc agcagatttg
    14701 atgagagagc aagaattgca tcgcttgcct gttatcgaaa atgatcaatt ggttggtttg
    14761 gtgactgagg gaaccattgc acaagcaagt ccatctaaag caacaagtct ttctatctat
    14821 gagatgaatt atcttctgaa taagacaaaa gtaaaagatg tcatgattcg cgatgttgtc
    14881 actgtctcag gctatgctag tctagaagat gcaacttatc tgatgttgaa aaataagatt
    14941 ggtattctcc ctgtcgtaga taaccatcaa gtatacggag ttattactga ccgtgacgtt
    15001 ttccaagcct ttcttgaaat tgcaggttat ggcgaagaag ggattcgtgt acgctttgtt
    15061 acagaagatg aagttggtgt tcttggaaaa attgtttctt tgattgtaga agaaaatttg
    15121 aatatctccc atacagtcaa tattccgcgt aaggatggta aggtgattat cgaagtgcaa
    15181 atcgatggat caattgattt accagccttg aaagaaaaat ttgaagcaaa tggtattcaa
    15241 gtggaagaaa tcgttcgcac ttcagcaaaa gtcttgtaag aagggaagcc caaaggcttc
    15301 ttttttcatg aaaaggggat tagagcaaaa gatggaaaga aatgataaaa tatgctataa
    15361 tgaaataatg taaaaaagga gtatttatgg acatttcagt aattcgtcag aaaattgacg
    15421 caaatcgtga aaaattagct tctttcaggg ggtctctttg acctcgaagg gctagaggaa
    15481 gagattgcca tcttggaaaa caagatgaca gaacctgatt tttggaacga taatattgcg
    15541 gcccaaaaaa tgtcgcaaga attaaatgaa ttaaaaaaca cttacaatac cttccataag
    15601 atggaagagt tgcaggatga agtcgaaatt ttattggatt ttttggctga agacgagtca
    15661 gtgcatgatg aactggtagc gcagttagcc gaacttgata agataatgac cagctacgag
    15721 atgactctac tcttgtcaga accttatgac cacaacaatg ccatcttgga aatccatcca
    15781 ggttctggtg gtactgaggc gcaggactgg ggtgatatgt tgcttcgtat gtatactcgt
    15841 tatggtaatg ctaaaggctt taaagtggaa gtgttggatt accaagcagg tgatgaggct
    15901 ggtattaagt cggtaacttt atcatttgaa gggcctaatg cctatggtct cctcaagtca
    15961 gaaatgggtg ttcaccgctt agtgcgaatc tcaccatttg actctgccaa acgtcgccat
    16021 acctctttca catctgtaga agtgatgcca gaattggatg atactattga agtggaaatc
    16081 cgtgaagatg atatcaagat ggataccttc cgttcaggtg gtgccggtgg acaaaacgtc
    16141 aataaggttt caacaggtgt acgtttaacc tacattccaa ctggaattgt tgtccaatca
    16201 acagtggatc gtacccagta tggaaataga gatcgcgcca tgaagatgtt gcaggctaag
    16261 ctctatcaaa tggagcaaga gaagaaggct gcggaggtag attctctcaa aggtgagaaa
    16321 aaggagatca cttggggaag ccaaatccgt tcttatgtct tcacgcctta tactatggta
    16381 aaagatcacc gaactagctt tgaggttgct caggtagata aggttatgga tggggaccta
    16441 gatggtttta tcgatgctta tctcaagtgg cgaattagct aagatagaaa ggaactcaca
    16501 tgtcaattat tgaaatgaga gatgtcgtta aaaaatacga caacggaacg actgctctac
    16561 gcggtgtttc ggttagcgtt caaccggggg aatttgctta catcgtagga ccttcaggag
    16621 cagggaagtc aacttttatt cgttctctgt atcgtgaagt aaaaatcgat aaaggaagtc
    16681 tatcagttgc tggttttaat ctggttaaga tcaaaaagaa agatgtcccg cttctacgtc
    16741 gtagtgttgg ggttgtcttc caagattata aattgttacc aaagaaaact gtctatgaaa
    16801 atattgctta cgctatggaa gtaatcgggg aaaatcgccg taatatcaaa agacgagtga
    16861 tggaagtttt ggacttggtt ggattgaagc ataaggttcg ttctttccca aatgaactct
    16921 caggtgggga gcaacagcgg attgcgattg cgcgtgcaat tgtaaataat cccaaagtat
    16981 tgatagctga tgagccaaca ggaaatctgg atccggataa ttcatgggaa attatgaatc
    17041 tcttggaacg gattaaccta caaggaacaa ctattttgat ggcgactcat aatagccaga
    17101 ttgtaaatac cttgcgccac cgtgtcattg ccattgaaaa tggccgtgtc gttcgtgacg
    17161 aatcaaaagg agagtatgga tacgatgatt agtagatttt ttcgccattt atttgaagcc
    17221 ttaaaaagtt tgaaacgaaa tggttggatg acagtagctg ctgtcagttc agtcatgatt
    17281 actttgacct tggtggcaat atttgcatct gttattttca atacagcgaa actagctaca
    17341 gatattgaaa ataatgtccg tgtagtagtt tatatccgaa aggatgtgga agataatagt
    17401 cagacaattg aaaaagaagg tcaaactgtt acaaataatg actaccacaa ggtatatgat
    17461 tctttgaaga acatgtctac ggttaaaagt gttacctttt caagtaaaga agaacaatat
    17521 gaaaaattaa ccgagataat gggagataac tggaaaatct ttgaaggaga tgccaatcct
    17581 ctctatgatg cctatattgt agaggcaaac gctccaaatg atgtaaaaac tatagccgaa
    17641 gatgctaaaa aaattgaagg tgtctctgag gttcaagatg gcggtgccaa tacagaaaga
    17701 ctcttcaagt tagcttcatt tatccgtgtt tggggactag ggattgctgc tttgttaatt
    17761 tttatcgcag ttttcttgat ttcaaatacc attcgtatta ccattatttc ccgcagtcgc
    17821 gaaattcaaa tcatgcgctt ggtcggagct aaaaacagtt atatccgtgg accgttcttg
    17881 ttagaaggag cctttatcgg tttattggga gctatcgcac catctgtttt ggtctttatt
    17941 gtttatcaaa ttgtttacca atctgtcaac aaatcgttgg tagggcaaaa tctatccatg
    18001 attagtccag atttatttag tccgttgatg attgccctac tatttgtgat tggggttttc
    18061 attggttcat tgggatcagg aatatccatg cgccgattct tgaagattta ggtaaaatag
    18121 ctgcttttat gaggagattg taaaatctcc ttttttgcta caagagtttt tgaaaagaga
    18181 tgcgcagaag aaaagagctt ccaaagaagt cccccagaga agacttcttg atggctaatt
    18241 tgaacttgaa atttggcgca ctagaa
//
LOCUS       DAVLVZ010000036        17205 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000036 DAVLVZ010000000
VERSION     DAVLVZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.036"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(34..264)
                     /locus_tag="VYN77_001290"
     CDS             complement(34..264)
                     /locus_tag="VYN77_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558746.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(282..923)
                     /locus_tag="VYN77_001291"
     CDS             complement(282..923)
                     /locus_tag="VYN77_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1558747.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(935..1669)
                     /locus_tag="VYN77_001292"
     CDS             complement(935..1669)
                     /locus_tag="VYN77_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1558748.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALIP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(1672..1869)
                     /locus_tag="VYN77_001293"
     CDS             complement(1672..1869)
                     /locus_tag="VYN77_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558749.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(1869..2075)
                     /locus_tag="VYN77_001294"
     CDS             complement(1869..2075)
                     /locus_tag="VYN77_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1558750.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(2085..2398)
                     /locus_tag="VYN77_001295"
                     /pseudo
     CDS             complement(2085..2398)
                     /locus_tag="VYN77_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2656..3945)
                     /locus_tag="VYN77_001296"
     CDS             complement(2656..3945)
                     /locus_tag="VYN77_001296"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1558751.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQVHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLIFEKQGVALPIENALDVYIA
                     VLGDGANAKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISKNFSEIFEKLGFYTQ"
     gene            complement(4023..5270)
                     /locus_tag="VYN77_001297"
     CDS             complement(4023..5270)
                     /locus_tag="VYN77_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1558752.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAVIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLDLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(5461..6312)
                     /gene="rgg"
                     /locus_tag="VYN77_001298"
     CDS             complement(5461..6312)
                     /gene="rgg"
                     /locus_tag="VYN77_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1558753.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            6436..6777
                     /locus_tag="VYN77_001299"
     CDS             6436..6777
                     /locus_tag="VYN77_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1558754.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            6835..6915
                     /locus_tag="VYN77_001300"
                     /pseudo
     CDS             6835..6915
                     /locus_tag="VYN77_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(7256..8959)
                     /gene="ilvD"
                     /locus_tag="VYN77_001301"
     CDS             complement(7256..8959)
                     /gene="ilvD"
                     /locus_tag="VYN77_001301"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1558755.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIIDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(9192..10124)
                     /locus_tag="VYN77_001302"
     CDS             complement(9192..10124)
                     /locus_tag="VYN77_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1558756.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(10121..10978)
                     /locus_tag="VYN77_001303"
     CDS             complement(10121..10978)
                     /locus_tag="VYN77_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1558757.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(10982..12328)
                     /locus_tag="VYN77_001304"
     CDS             complement(10982..12328)
                     /locus_tag="VYN77_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1558758.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(12341..12625)
                     /locus_tag="VYN77_001305"
     CDS             complement(12341..12625)
                     /locus_tag="VYN77_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1558759.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(12629..14659)
                     /locus_tag="VYN77_001306"
     CDS             complement(12629..14659)
                     /locus_tag="VYN77_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1558760.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            14850..15857
                     /locus_tag="VYN77_001307"
     CDS             14850..15857
                     /locus_tag="VYN77_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1558761.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            15860..16060
                     /locus_tag="VYN77_001308"
     CDS             15860..16060
                     /locus_tag="VYN77_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1558762.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            16212..16394
                     /gene="rpmF"
                     /locus_tag="VYN77_001309"
     CDS             16212..16394
                     /gene="rpmF"
                     /locus_tag="VYN77_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1558763.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            16410..16559
                     /gene="rpmG"
                     /locus_tag="VYN77_001310"
     CDS             16410..16559
                     /gene="rpmG"
                     /locus_tag="VYN77_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1558764.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(17039..>17205)
                     /locus_tag="VYN77_001311"
     CDS             complement(17039..>17205)
                     /locus_tag="VYN77_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558765.1"
                     /translation="DKGLWYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGY
                     RVNANGEWVG"
BASE COUNT         5584 a         3603 c         2952 g         5066 t
ORIGIN      
        1 atacaaaaat ccgatgaact tcaccggact cttttatttt gaatttttgc ctgctttacg
       61 cttttcagcg atttcggctg cctttcgagg caagacaatt tccgttatgt aagccgtccc
      121 aaaacgcagt acacctgcaa taggagcaaa gacaactgct agatagttat agaagaaatc
      181 gcctttgaag gcataagcta gcgctccaat gatgaaaaat agaacgactg cctgaatcac
      241 tgctaataaa attactcgtt tcatgtgacc tcctgactct attatagcat gagaatcatc
      301 aaaaagccga ctaaattatt caaagcgtga agagaaatac tgtagaccag accttttctg
      361 ctaacgtaag ccaaacccaa actaaaacca aggctaaaat agacaaaaaa ttgttgcaca
      421 tcacctggaa aatgaatcaa ggcaaataga agactagata ccagaagaaa aatcagggtt
      481 cgtttactat tgtcctgctt aggaaagaga tagcgtgcta acatccctct aaaaacaatc
      541 tcttccgtca aaggagcaaa aataaccaca gcaaagaatg agaaaagtgg ttgagacaag
      601 gtcaagtctg tcgctatttg ctgatttact gaaggatcat ctggcaagaa gaattgaacg
      661 accagagata aaaaccaaac caaggcagga agccaaataa atcggttaaa gccgctcttc
      721 tcaatatgaa caggagcctt ctgataccat ttgtaaatgc cgtacacata tactccagcc
      781 aaggccacat agagtagagt aacagcatag ggtgaagcgc ctaaagcaag cgacgcagtc
      841 gcgagcccct gaataaagcc atagataaat aaaaaggata gaagggctag aagaatccag
      901 ccaaggtttt taagtaattt cataaataac tcctttattt gaaataacgt tttaccatag
      961 gtaactgcat cacattgata taaacatgga tggctcctac aagcaagaaa gctagtaact
     1021 gaatctctcc tgtcaagaaa gaaatgataa taagaaaaat atataaggct ggtaagacat
     1081 attggtgtaa ttggaataaa attcgaaaac tctgttccaa attagcctga cgctcccctt
     1141 catcataaga atttatatag ttcaagacat cctttggtgt agcgaaaaat tccaaatcaa
     1201 actgacgaac aatcgcaatg gttttaaaaa gagatttttg agcgactaag aataccacaa
     1261 agagtaaaaa agaaaggaaa aatgtttgag ggtttgtatg caatataatc acctcactta
     1321 atgaaataaa aatagccaat ggaatcgcta cacctgtaat attaaaagca atggttccaa
     1381 actcaagatt ccgatacatt tgcacataat aggtttcatt cagatcgtca tccatctcct
     1441 cttgatacaa ggaatgaaat tttctgcttt tctttaagaa attgaaagtc aaaaacatac
     1501 taatgaaacc tatcagtaaa caaatagctg atatccatgg tatcaaggct tttacatcta
     1561 aaataatttc gtgggattcg acacgtgcct taaacatccc tacaaacatg cccaagaacc
     1621 cccccaagac aatagacatc aaaaataaca atctacgttt ctttttcata ttcattctcc
     1681 tttttcactt gctagatttt tggatttctt ttcaatccat tcaattactg ggatgagagc
     1741 aaagtagacc caaacaaatt ggtcgctttg atagggatta aaccagctta ggtccatccc
     1801 aatcagtaga aatacactga ctaataaagc tatgaccact acataataaa tcactttata
     1861 cttgttcatc actcgtcctc ctccaaacga aataccgatt cgactgtttc gttgaaaatt
     1921 tgagatattt tcagggcaat gataatggat ggggtgtact catcccgttc tagtaggcta
     1981 atggtctgtc tggaaacccc tgccagtttg gctaggtcgg tttgattgag accatcgcga
     2041 gctcgaagct cttttagacg attttttagt tgcatgttac acacctactc tccgtcaaat
     2101 tcaacggttt ggatatcttc aatacgttgc aacttgaatt tttctttccc ctttttatca
     2161 acactacgta gtttgaccca ttcctcgtca acatccacaa cttcccagtt atctggccca
     2221 atatacactc ccgttataat tggttccttt ccaatcattt cttgtaataa tctcgacatt
     2281 tctgcgtttc ctttctcttt tcgctcaagt tttttgattt tattctctag tttcttgatt
     2341 ttttagaatt attagaataa aagaaaatca taaatagtat aaatactagt acccacatta
     2401 taactccttt ctgcttccta tttcttaact tgaattcatt gtaacatatc tttttctttt
     2461 tgacaagtat agttgtcaaa aagtttatga tttttgtcat tttgcaaaag aaaaaggtca
     2521 ggagtaagtt cctgaccact ttatctatca ttaatactct tctaaaatct cttcaaacca
     2581 cgtcagcttc acctacaacc tcaaaaccat gttttgagca acctgcggct agcttcctag
     2641 tttgctcttt gattttcatt gagtataaaa tcctagtttt tcaaagattt ctgagaaatt
     2701 tttgctgatt gtctcaagtg acacttgcac ttcttctcga gtttgattgt tcttgaccgt
     2761 cacttgtccg ctttcgactt cactctctcc tagagtgatg agggtcttag ccgcaaagac
     2821 atcggctgac ttgaattgag ctttgagttt acggttgagg taatcacgct ctgctttgaa
     2881 accttgttgg cgaagagctt ggactagttc tagggctttg gcatttgctc catcacccaa
     2941 gactgcgata tagacatcta gggcattttc gatagggagg gccacgcctt gtttttcaaa
     3001 gatgagaagc aggcgctcta caccaagccc aaaaccaaat ccagcagttt cagggcctcc
     3061 aaagtaagca accaaaccat cgtagcgacc acccgcacag actgtcaagt cattgccctc
     3121 aatctctgtg ataaactcga aaatggtgtg gttgtagtag tccagaccac gcaccatatt
     3181 ggtatcgatg atgtaatcta ctccaagatt ttccaacatc tgacgcacag catcaaaatg
     3241 aacttggctt tcttcatcaa gaaagtccaa gatagacggc gcattctcta ctgccacctt
     3301 gtcttctttt tccttagagt ccaagacacg aagaggattt tcctccaagc gacgttggct
     3361 atccttagac aaggtctcct tgagcggtgt caaatagtca atcaaggctt ggcggtaggc
     3421 tgcacggctc tcaggatttc caagagtgtt gaggtgcaat ttgacacctt gaataccgat
     3481 ttccttcaaa aaatgggctg ccatagcgat tgtttccaca tcggtagctg gattgctaga
     3541 gccaaaacac tcaacaccaa tctggtggaa ttggcgcaag cgccctgcct gtggacgctc
     3601 ataacggaac ataggtccca tgtagtagaa cttgcttggc ttttgcactt ctggggcgaa
     3661 gagtttattt tccacatagg aacggacaac gggcgcagtt ccttctggac gaagggtaat
     3721 atggcggtca cccttgtcat agaaatcgta catttccttg gttacaatat ctgttgtatc
     3781 tccgacagag cggctgataa cctcgtaatg ctcgaaaata ggcgtacgca cttctgcgta
     3841 gttgtagcgt ttgaaaattt cacgggcaaa gccctcaacg tactgccact tggcagattc
     3901 agcaggtaaa atatcctgcg ttccttttgg tttttgtaat ttcataggga atcctcttta
     3961 aacttaatag tcttatttta ccataaatag agggattaaa acagtgagaa aaaaattagg
     4021 atttagatat catttttgag attaagaatt gttaaaaaaa tagctagcaa ggaaagacca
     4081 acaaatagca tccaagtcaa ctgcatgttc catacggcta ctagtgaaaa acaagctgtt
     4141 cccacaggta tggataaggt aaacaataga tctaaaaaat tactagtacg agctagaacc
     4201 tctggagcta gatttttcat gagcatggca ctaatctttg gttgaacttt accagacaca
     4261 tacagagtaa agaagagaaa tagcaaacca agcacgactt gattgaataa attagccaaa
     4321 ccaactagac taagtcctac ggtcgcccac atcatcaatc taggcaagga ctgcttccca
     4381 aaataatcat tgcccgtaag gctactgatg atgactgcta ctaaaacaca gaattgattg
     4441 ataaatagtg cctctgtata agaaaaattc aagagagaat ggctcaaaaa gaagatatta
     4501 taaattccac ccaaagcgcc acccaaggaa ttaataagca agacagcaaa gagcataaaa
     4561 ccaaagtttt tctgtccact tttaagaaaa acgagacgta aatttcggta aattgttagg
     4621 aactggtctt tgatagaaag cttctcattt tttaagtttt caccatcagc agatgacatt
     4681 gacaggctca atttgctttt tcctaaaaag aggatagtgg ctgatactag gaaaaagcag
     4741 gcattgattc ccgcaacgag agaaaaattg ttgaccgata gagctaagag ccagactccg
     4801 aaagcttgac caccaatagc tgaaatatag gtgatgaact gtgaaaaaga ataagcctcc
     4861 atcagatcat cttcagctac tttttcctta ataagaggca tacgcaggcc acctgcaaaa
     4921 tcactgatga tatcactaat gacattgatc aaacacaggc tagaaaaggc aaagagacta
     4981 gcttgctgaa caactagggc tgctagaaaa aatagaaccg cctgaaacaa accgctatag
     5041 accatccatt tgaccttgtc cctcgtgtaa tctgcccgaa tccctgcaaa aactgtaaag
     5101 aaggtcggaa gaatcatgac aatattcgcc atagcaacag caaaagatgc ttgtgacaag
     5161 gtcgatgcat agacgataaa gaccaggttg aaaatcgaag caccaaaagc attgaagaag
     5221 cgtgataaag tcaaaattct ataagctgga tttctaaaca atagtttcat agataatctc
     5281 cctccttgtt ctaagttcta catcaagaat agctttagta tagcacttat gaatttgcga
     5341 aacgattctt ttgaatatat gcaacaaaca aaatgagcaa aaaaacgata gaacttttgt
     5401 aatgacctga gagagggcaa cagttctatc gcttcaaatt ttttcttggt ttgcagatat
     5461 tcaacattcg gtcgtatttc tatagtattc ggcagattta ttacagccca gcatctcaaa
     5521 aatacggaca gcatcctcca tctttttctg gccttccttg actctgcctt gcttgctatc
     5581 aaggagacct tctgcccaca gataaacaat tcggaaatag gtctcatttt ccttgtagaa
     5641 atgctcttcg ataacacgtt taaaataata ggcattggta aattcttcac actcaatact
     5701 agctaaaaag ccattcaata gtatagtatg aaaaaggttt cgattgccag acatttccat
     5761 tagaaaatca gatttacgta ccatttctcg tacatatcta gtaaaaagag aaacagataa
     5821 aaatggagaa ctgactgaaa ataaattgag ttcatagatt ccccagatct cggtagaaaa
     5881 caaataatca tgaaggactt ttccttcctc tgctgttaac tctacccttt catctatgct
     5941 cttcatataa gacttgatca taatggcatt tagaatatgt ttctgtttgt tgtgagaatg
     6001 ggcatgcttt tgatactccc tgcgatataa gtcctcaaga ggtgctatat tctttggttc
     6061 caagacatct gtaatttctt ttctcaactc agaatctgta tcatactgga aacctctcgc
     6121 cagaaagagg atctcctcca cactggcaga tatattttcc agagcaaata gaaacttttc
     6181 caccgaaagc tcactctgac ctgtttcaaa acgggacaac atagacggcg aaaattgtcc
     6241 tccggttgct tgtctcagtg agatatttct tgactctcgt aattgtctaa agacttttcc
     6301 aatctgctcc atagacttcc ccttgattcc gtattttctt cattttatca tatttttcag
     6361 aaaattcatc aaaaacttgc caaattgtca gaattatgag aaaatagagg atatttatca
     6421 cgtggaggga ctgctatgag agacgatatc aaaatcaatg accgtgcttt ggccttgcaa
     6481 gaccaaatta tcgaaaaact agagaaagtt tttgatacag atgtggaatt ggatgtttac
     6541 aatctaggtc tgatttatga aatcaatctg gatgaaacgg ggctctgcaa gattgtcatg
     6601 accttcaccg atactgcctg tgattgcgcc gaaagcctgc ctattgaaat tgtggcaggt
     6661 ctgaaacaaa tcgagggtat caaagatatc aaggttgaag ttacctggtc gcctgcttgg
     6721 aaaatcacac gaatcagtcg ctatggccgt attgcccttg gactaccacc tcgttaagca
     6781 ggccaatcac ttttaaagat gaaaaaagca agccgaaaat ggcttgcttt ttgatactca
     6841 atgaaaatca aagggcaaac tagaaaacta gccgcaggtt gctcaaaaca ctgttttgag
     6901 gttgtggata gaactgacga agtcagctca aaatactgtt ttgaggttgt ggatagaact
     6961 gacgaagtca gctcaaaata ctgttttgag gttgtagata gaactgacga agtcagctca
     7021 aaatactgtt ttgaggttgt agatagaact gacgaagtca gctcaaaata ctgttttgag
     7081 gttgtagata gaactgacga agtcagctca aaacactgtt ttgaggttgc agatagaact
     7141 gacgaagtca gcccaaaaca ctgttttgag gttgtggata gaactgacga agtcagtaac
     7201 catacctacg gcaaggcgac gttgacgtga tttgaagaga ttttcaagta tgagtttatt
     7261 ttttacctga cttgtccata ttccagaagt ctgtcacggc tccgcgtgaa gcagatgata
     7321 cgatatgggc atatttaccg aggacaccac ggctgtaaag tggtggcaag gttgtttctg
     7381 ccttgcgttt ttcaagttct tcttcggata cggccatgga aatttctttg gtatcttggt
     7441 caaccgtaac gatatcgccg gtacggagat aggcaattgg tccaccatcc tgagcttcag
     7501 gagcgatatg tccaacaacc agaccataag taccaccaga gaaacgtccg tccgtcaaga
     7561 gggccacctt atctccctga cctttaccaa caatcattga agaaagtgac agcatctcag
     7621 gcataccagg accaccttta ggtccaacaa aacgaacaac gactacatcg ccatcaatga
     7681 tttcatctgt caggacggcc tgaatcgcat cttcttctga gtcaaagacc ttagctggcc
     7741 caacgtgacg acgcacttta acacctgata ccttggcaac tgcaccgtca ggagcaaggt
     7801 tcccgttcaa gatgataagc ggaccatccg cacgttttgg attttcaagt ggcatgataa
     7861 ctttttggcc tggagtcaag tctgcaaagt cagccaagtt ttcagctaca gtcttaccag
     7921 tacatgtgat gcgatctccg tgaaggaaac catttgccaa caagtacttc ataaccgcag
     7981 ggacaccacc gacttcgtag aggtcttgga agacatactg accagatggt ttcaagtcgg
     8041 ccaagtgagg cacacgttct tgaatcgtat tgaagtcctc aagtgacaag tcaacatttg
     8101 cggcatgggc aatggcgagc aagtgaagag tggcgtttgt agaaccaccg agagccatcg
     8161 ttacagtaat agcatcttca aaggcttcac gagtcaagat atctgatggt ttgagaccaa
     8221 gttccaacat cttaacaaca gcacgtcctg ctgcttcgat atcttctttc ttatcagctg
     8281 attcagctgg gtgagaggat gaccctggca aactcatccc tagaacttcg atagcagttg
     8341 ccatggtatt agcagtatac ataccaccac aaccaccagg gccagggcag gcattacatt
     8401 caagacgttt cacgtcctca gctgtcatgt caccgtggtt ccattttccg ataccttcaa
     8461 agacagaaac caagtcgata tctttaccat caagatttcc cggtgcaata gttccaccat
     8521 aggcgaaaat agctgggata tccatattag caatagcaat catagatcca ggcatgttct
     8581 tgtcacagcc accgatagcg acgaaggcat ccacgttgtg accactcata gccgcctcga
     8641 tggagtccgc gatgatgtca cgagatgtta gagagaaacg cataccaggc gttcccatag
     8701 cgatcccgtc cgctacggta atggttccaa actgtacagg ccaagcgcct gcagatttga
     8761 caccttcttt agccagtttc ccgaaatcat gcaagtgaat gttacatggt gtattttccg
     8821 cccaagtcga aatcactccc acaatcgatg tttcaaagtc cttatctgtc ataccagtcg
     8881 cacgaagcat agcacggtta ggtgatttaa ccatgctgtc ataaatgcta ctgcggtgac
     8941 gtttatctaa ttcagtcatc ttatccctcc catttcagtt tttactatta tagcacaatt
     9001 taaaggcaat gaacagaata aaattcttga attttcagaa aattctatac gcatgtgaaa
     9061 tatttaaaat taaaaacaac aaagcggatt agtgcacttt ctgatgacca gaatatgctt
     9121 tttaatccgc tttctttaaa taacgtactg taatttttac agaaattctt tcagataagt
     9181 gtatttaaca tctatcttgc attataaatt tctagaacct tctcttttat attcgattca
     9241 ctcaaaccat actcattaag aagataatcc attttcccta cttgaccgaa tctttcttga
     9301 acacccatcc gatgaatttt tgttattcca tcatcagaga ataattcaca taaagcactg
     9361 ccaattccac ctatctgatt gtggttttct acagtaaata tagtttttcc acttaacatt
     9421 gtttttatct gttctggtat cggtttgatt ctaaataaat ctatcacacc tactgaataa
     9481 cctaatttag acagttcatc tgcaactcga atacttggag caaccattat gccagaagca
     9541 acgattacaa gatcttcacc atgtcttaac tcaatgtagc ccttagaaaa atcttctcca
     9601 ccttgatata cagcctctgg agcttttcta attgttcgaa tatattttag tccttttaag
     9661 tctaatgtct gattcaatat ttcacgaaat tggatatcat cagttgcttc aaaaatgatt
     9721 gatttaggaa ttaaacgtaa caatccaatt tcttcaaatg gcatatgtgt cccaccattc
     9781 atttctgcag ttactcctgc atctgatcca atcacagtgg catccaattg tgcgtatcca
     9841 agagaaataa ataattgatc aaatactctt cgtgaagcaa aaggaccaaa tgtatgaaga
     9901 taaggtctaa acccctgaat agacaagcct gctgcaaggc cgaccatttc tgcttccata
     9961 atcccaacat tcacataacg gtctccaaag tccttttcaa gattatgagt agccatcgaa
    10021 cttgacagat cggcttctaa gactactata tcagaataac tttgattagc ctctaaaagg
    10081 aagtctctat atacatgccg taattctttc gtacttctca tcattctgtt tcctccaatt
    10141 cctgacttaa tctttctaca actgaagtta acatttgtct ctcctctaca gtagggcgaa
    10201 gatgatgatt ggatttcatt tcttccagct cttgaacccc ttgaccttta atagtatcta
    10261 atacaatgca cttaggtgat gaattatttg actgttttaa ttggacaatc ccttcataaa
    10321 tttctctaat atctgaaccc ttgaccctaa tggattcaaa tccaaatgct gaaaattttt
    10381 ctacgaaatc acctggatta caaatatcct ttgtaaaacc atctaattgt tttttgttat
    10441 catcaacaaa tacaattaag ttggataact gttgatgaga agcaaactgt atagcctccc
    10501 aacattgtcc ctcatttaac tcaccatctc caacaatagc ataagtataa aagggactct
    10561 ttcttattct ctgaccatat gcaagtccag ttgcaacact aattccttgt cctaaagagc
    10621 ccgttgtcat atctatgcct ggcgttagat ttctatcagg atgagacggt aatttggttc
    10681 catttgtatt taaagaatat aagaattctt tgtcaaagaa accattcaaa tagagtgtac
    10741 tgtatagagc tggtcctcca tgaccttttg ataatatgaa ataatctcta tctcgtgctg
    10801 caaatatttc tggagtcatt ggcattattt caccataaag cactgctaaa acttctacga
    10861 tagacagact tcctccgtaa tgtccgaatc caagatgatt caatgttcta agagtattta
    10921 atcggatgtt agtcgcaaat tttcttaacc catcttctct atttttactt aaaatcatcc
    10981 cttattcctc ctttgcagat ggctttttaa taaaggatac tccaaacata actgctagaa
    11041 taagaacaag accaatcaca atgcctgctt gtgagccaaa ttgatttaac attcctaaaa
    11101 taattcctga tagaccaaaa tctgcatctg agaaagttga tccttggaaa ccaagtcctc
    11161 ccaaaactgg cattaaaaag actggaagaa aactgattaa aataccttgt aaaaatgctc
    11221 caatagtggc tccacgaaca ccaccagatg cattcccaat gacacctgca gtcgctccac
    11281 agaagaaatg aggcacaaca cctggtaaga taacaaccgt tcctgaagca atcataattg
    11341 ccatacttac taaaccacca ataaaactag agataaatcc aattagaact gcattgggtg
    11401 cataagtata aacaatcgga caatccaaag caggttttga attaggtaca agacgctctg
    11461 aaataccttt aaaggctgga acaatttcgc ccaaaataag gcgaacacct gctaaaataa
    11521 caaatacccc tgctgcaaat tgacctgcta attgtaaagc ataaactaga ccacttgtac
    11581 cactactgat ttctttttct atatattctg accctgcaaa gatagctaca ataatgtaaa
    11641 taactgccat agataaagtg atactaacag tactatcacg taaaaaagct aaactctttg
    11701 gaaatttaat gtcctctgtt gattttgatt tgtcaccgat aaggctacca gtaaaaccac
    11761 tcaaccaata tcccaaagaa ctgaaatgac ctaaagccac cttgtcattt ccagttaatt
    11821 gaaccatata tttttgcaca aatgctgggg aaatactcat aataataccg agtgctaatc
    11881 ctcctagtaa gatgagaggc aagctagtaa agccagcaac tgataaaatg accgcaatca
    11941 tacatgccat atatagagtg tggtgccctg ttaaaaaaat atatttaaat cgagtaaaac
    12001 gagcgattaa gatattgaac accatgcctg caaacataat cattgcagta gctgagccat
    12061 atgttgttaa agctacagct acaattgctt cattattcgg cacaacgcca gataaatgaa
    12121 aagcatgctc aaacatggta ccaaatggat ttaaagaatt ttgtacaatt cctgcaccac
    12181 cagatacaac taagaaacca acaaaggtct taattccacc tttaataata tcaggtaatt
    12241 tcttcttctg aagaactaat cctaaaattg caattaaagc tactaaaata gctggtgtac
    12301 taacaatatc caatatgaac ttcatcatga cgctagcctc ctatataagt cctttttctt
    12361 cacaaagttt agtaattaat tctcgtagtt catccatatc aataatacta tttaagatac
    12421 gaacatctcc aagatgacta gctgaatcag ctagatcacg accaacaatc caaatatcag
    12481 ctgcatttgg atctgctcca cctaaatcat aatgttcaac ttctacatcc gaaacattca
    12541 aatcactcaa tacagattca atattcatct gtaccataaa acttgaacct aatcctgaac
    12601 cacaagctgt accaattttt aacattatct aatcctcctg tttaattatc attttaatgt
    12661 catcatagtt ttttgatgat attaaagttt gaacatgatt tttatctctt aaaattgttg
    12721 ttaaatgtga caaagccttt aaatgactct cattatcaat ggctgcaata caaatcaaca
    12781 atcttacctc ttgttctgga ttatccaata aataaatcgg ttcttccaaa actaacattg
    12841 acattcctat ttcattcaca ccttcatctg gccgagcgtg aggaattgct actcccttcc
    12901 ctaaattaat aaaaggtcca aactcttcta ctttttgaat cattgcctca gggtagttct
    12961 cagttatctt atcttgatcc aaaagcggtt tagctgctaa acgaatcgcc tccttccatc
    13021 ctaatttttg cgaactaacc tgataggttt ctttggtaat aagttgttct agcactggta
    13081 caatttcctt tctatcattt ttttggtaaa gataattctt taacgccaat cttaattcca
    13141 attcttgtgt aataattcca tatcttttga caatattcag gatttgttca atctcaaaat
    13201 ctccatactc taaattcgga aaatctttta acactagttc tactagttgt attgcttgct
    13261 cttcagtcat cataaccgaa actagataat ttggcttttc tgtctccacc tttatggtag
    13321 aaaaaaccat atcatagtca ctactagctt tcacctgtaa atcatcaatc tttgaggttc
    13381 ctataaactc aatttgagga aataatgcta atagattctc ttttaacatc aatgaagaac
    13441 taacaccatt aggacaaatg attgctgctt tatagcattt ttgaggcaaa gtatctgctt
    13501 tctttaaata acctccgaaa tggataacaa aatatgctgt ttcactatca ggtatgggat
    13561 tgtcaatagc gtccatcaag ggcatcaaag aatctttgac taattcaaat aaatcaggat
    13621 aatgttcttt aacatgcaat acatattcat ttgaactagg taggccgaac tttaatctat
    13681 agtaagccgg tataaggtgg cggcgaagat tttctctcaa tccttccctt tgtttaaaat
    13741 gtaacaaaga aatatcttcc attctactta taatagcctc tgttaattga ttaaagtaaa
    13801 ccggagcaac atctacttca ccttcaaagc aacttgataa taaaactgtg atatagcgat
    13861 aatcatcctc agaaaacacc gtatctataa ttcccaaatc aaccactgta tccaataaaa
    13921 tagtggttat atcttgaata acaggagata ctaatgtctc tgaaagacat actctttcaa
    13981 catccctttg atacctacac agaatgaata ctaaaccgaa aaggcaaact tttaattgat
    14041 taacaatagg tactagttgt agcttctcat aataatcttt aactacctga tttatcaaat
    14101 cataagttaa tgaatacccc caactggata aaacataatc caaaccccaa atccctatgg
    14161 aggattccag caactcacta accatttgaa aagctaagcg gtgcttattc cactctgaac
    14221 cgtgtaaagt ataacctttt gctctactgt accctagctc caaatcatta tctaacataa
    14281 tctttcttaa tgattgaata tcagataagg ttgtattctt acttactttc aaaaagtctt
    14341 ggtaatgatt attcgatata aaatctaatc ggcaaaaagt gtaaagatag attaaagcta
    14401 agcgagtcga ctttggtaaa accaattcat ccgacttaat aatatctgtc aaagactgct
    14461 tcgtacgatt tgataaacta tagcgacctt gttttttatc cagcactatc cctttattag
    14521 ctagataagg cactaaataa tctattcctt ctttgacttc ctttataggt aagctcacct
    14581 taacagataa ttcatataat gatagctcac aatgatccat caaagtcatc aaaataacta
    14641 gtgctctata atcaaacatt tctcttcctt tctaactaca attatagtgt aaaaattata
    14701 agaataaaag agtatttgag cacaatataa tctcaccttt taaaagaaat tattgggctg
    14761 aaagttttta gtgattattt tcaactttct attgactttt aaaaacaaag tgatatcatt
    14821 tagatatcaa ataatgggag gtcattctta tgactgaaaa actaatcaat tcaaaaccaa
    14881 atggtgtatt cgcattgatt ctcattgagt tgacaatcgt acttggtgtc tttatattta
    14941 taatgggtgt tggttcggaa aacatttttg gaattattat cggaccttta ctaatcgtaa
    15001 ttgcaggtct agctcatgct ggtttaaaag ttgtcaaacc tcaggaagct ctggttctga
    15061 cactctttgg taattataca ggtaccatca aagaacctgg tttttacttt gtcaatccct
    15121 tcagcgtagc agtcaaccct gcaaaccaca ctcgacttgg acaaagtggt gatgttagca
    15181 caaaatctcc ttttttagga gctaaatcat caaatgacaa tgatgtaaat cttgaaattg
    15241 gcaagaaaca catttccctc aaagtcatga ccttgagcaa ttctcgtcaa aaaatcaatg
    15301 attgcttagg aaaccctgta gaaatcggta tcgcggtaac ttggagagtt gtcgataccg
    15361 ctaaggcagt cttcaacgtt gataactaca aagaatatct ttcactgcaa tgtgatagcg
    15421 ccctccgtaa tattgtccgc atctatcctt acgatgtgtc tcctaatgtg gatactacgg
    15481 gtgacgggca agcagatgaa ggtagtctcc gtggctctag cgaaattgtt gctaaccgta
    15541 ttcgtgaaga aatccaaagt cgtgttgagg atgctggctt ggaaatcctt gaagcacgta
    15601 tcacttacct agcttatgct ccagaaattg ctgccgttat gcttcaacgc caacaagcat
    15661 ctgccattat tgatgcacgt aagatgattg tagatggtgc tgtaggaatg gttgaaatgg
    15721 cactagaacg gctcaatgaa ggggaattgg tagaacttga cgaagaacga aaagctgcca
    15781 tggtttcaaa tctccttgtc gttctttgtg gcaatcatga tgcacaacca attgtcaaca
    15841 caggaagtct ttactaaaga tggctgaagc taaaaaaaag cagatccccc ttcgactctc
    15901 aacaaagtta tacgctgcac tcgcatcatg ggcagaagac gactttcgtt cagtcaatgg
    15961 ccaaatcgaa tatctcctca cagagtgtgt caagcaacgg aagaaagacg gaaaatacgt
    16021 atcagaaacc attgacgaac catttgagat tgatatttaa caccttctcc tgtctgccgt
    16081 gtcggatagg agaatttttt catctttttt tgtcaagtaa ctttacttta caaaaaaaat
    16141 gtgttatcct agtatggttg atgaaaatca gtagattgaa tcgaatataa atacctaaag
    16201 gagaaatcaa aatggcagta cctgcacgtc gcacttcaaa agcgaagaaa aacaaacgtc
    16261 gtacacacta caaagtaaca gctccatctg taaactttga cgaaactact ggagattact
    16321 cacgttctca ccgtgtatca cttaaaggat actacaaagg acgtaaaatc gctaaagctg
    16381 catcagctga ataatagaag ggagatacca tgcgcgtaaa tattacactt gaacacaaag
    16441 aatctggtga acgcttgtac cttacttcta aaaacaaacg taacactcca gaccgccttc
    16501 aattgaagaa atactcacca aaacttcgca aacacgttgt gtttacagaa gtgaagtaag
    16561 cattcaatac gaatcaatac agaagaaaaa cgcttgatca gcgttttttc tttttgttaa
    16621 tttcactata ttgcaagaga aatcattcga atgactacat tttttgaaat aagaatacgg
    16681 attcggacga ttaaaagtaa gagaaactca aatgtaagtt ggggttcctt ttttgtatat
    16741 ttttcaaata acgcctctac actatttgta gcaaattcac caactacagt tgcatcttag
    16801 ttaaaataag ttagaatatg taagtgagta ccagatatac caagacatcg tcaccatcta
    16861 aggtatattc aaaatacaaa agttgaccaa ctagatttct gaatatcctt atatatccat
    16921 tcttaaaatt ggtttaaata gcgtagtctt ttaaactagt tttgagaatc caaaaaatct
    16981 tcctacatat gtaagaagat tttttagttc agaatgatta gatttagcta atggatacct
    17041 atcctaccca ctcaccgtta gcattgactc gatagccatc gggtgtagtc gtgtttacta
    17101 ataaatctcc tgaattatag gtatagtacc atttaccaga aactgtaaac caaccagtag
    17161 ccattgaacc tgattcattt aagtaatacc ataagccttt gtctt
//
LOCUS       DAVLVZ010000037        17081 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000037 DAVLVZ010000000
VERSION     DAVLVZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.037"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..424
                     /locus_tag="VYN77_001312"
     CDS             <1..424
                     /locus_tag="VYN77_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558766.1"
                     /translation="GISYFQILESCTEKSISIEKRGKSKPIDMSDIISICDNGLGYET
                     SMVLNAINNILEKLVGEELDVLTMLIKRLKYGLPLEKEINIYELGFSDRIVVQVIGQE
                     INSVSKNQIRNEIKQKSARLEDKLSDFPSYYTQIINEM"
     gene            449..1345
                     /locus_tag="VYN77_001313"
     CDS             449..1345
                     /locus_tag="VYN77_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV1558767.1"
                     /translation="MTKISFEIQQQIIQCFGLCFHYKDTVVSFMQTSGVPNDLILKWK
                     SEPKFVWAKNVINELNKTENGRLIIRRIATEFYKMKNIPDEVQDRDRGLDALRKLKRL
                     IGDTQQNKVNETLNNSYHRSRQEMKIQLRQQLLQKIEELKTEYYSLFSSDNPQERGYR
                     LEKIVANLFRINDIDYHDSYRNRTNTQQLDGYFRFEGFDYLVEMKWEKNPVNSPKIAS
                     LKQKVDTKLTSTRGLFLSINGFRDEVIQDFSNKDAKILFMDGQELAYILENRISLYEA
                     LKVKIIGASKTGNPNVSIINQE"
     gene            1748..1986
                     /locus_tag="VYN77_001314"
                     /pseudo
     CDS             1748..1986
                     /locus_tag="VYN77_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            2130..3359
                     /locus_tag="VYN77_001315"
     CDS             2130..3359
                     /locus_tag="VYN77_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1558768.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            3379..6075
                     /gene="ppc"
                     /locus_tag="VYN77_001316"
     CDS             3379..6075
                     /gene="ppc"
                     /locus_tag="VYN77_001316"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1558769.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    6158..6375
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6488..7492
                     /locus_tag="VYN77_001317"
     CDS             6488..7492
                     /locus_tag="VYN77_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1558770.1"
                     /translation="MKNKRLIGIIAALAVLVAGNLIYSSMNKSEVQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSIGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7561..8427
                     /locus_tag="VYN77_001318"
     CDS             7561..8427
                     /locus_tag="VYN77_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1558771.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            8424..9182
                     /locus_tag="VYN77_001319"
     CDS             8424..9182
                     /locus_tag="VYN77_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558772.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            9272..11032
                     /gene="dnaG"
                     /locus_tag="VYN77_001320"
     CDS             9272..11032
                     /gene="dnaG"
                     /locus_tag="VYN77_001320"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1558773.1"
                     /translation="MVNKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQDLYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            11035..12144
                     /gene="rpoD"
                     /locus_tag="VYN77_001321"
     CDS             11035..12144
                     /gene="rpoD"
                     /locus_tag="VYN77_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1558774.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            12158..12487
                     /locus_tag="VYN77_001322"
     CDS             12158..12487
                     /locus_tag="VYN77_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1558775.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12602..13645
                     /gene="cpoA"
                     /locus_tag="VYN77_001323"
     CDS             12602..13645
                     /gene="cpoA"
                     /locus_tag="VYN77_001323"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1558776.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKEIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13661..14986
                     /locus_tag="VYN77_001324"
     CDS             13661..14986
                     /locus_tag="VYN77_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1558777.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFSDVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDVNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            15017..15193
                     /locus_tag="VYN77_001325"
     CDS             15017..15193
                     /locus_tag="VYN77_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1558778.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            15252..15386
                     /locus_tag="VYN77_001326"
     CDS             15252..15386
                     /locus_tag="VYN77_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1558779.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            15450..16760
                     /gene="obgE"
                     /locus_tag="VYN77_001327"
     CDS             15450..16760
                     /gene="obgE"
                     /locus_tag="VYN77_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1558780.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16770..16928
                     /locus_tag="VYN77_001328"
     CDS             16770..16928
                     /locus_tag="VYN77_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558781.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         5369 a         2909 c         3765 g         5038 t
ORIGIN      
        1 ggggatttct tattttcaaa tattggaaag ttgtactgaa aaatcaatta gtattgagaa
       61 aagaggtaag tcaaaaccaa ttgatatgtc tgacataatc tctatttgtg ataatggatt
      121 agggtatgaa acatcaatgg ttttaaatgc gattaataat attcttgaaa aactagttgg
      181 agaggaattg gacgtattga ctatgttaat aaagaggctg aaatatgggc tacctctgga
      241 aaaagaaatc aatatctatg aattaggttt ttcagataga attgtggtcc aagttattgg
      301 tcaagagatt aattctgtaa gtaagaatca aattagaaat gaaatcaaac agaaatcagc
      361 tagactagaa gataaattga gtgattttcc aagttattat acacagataa ttaatgaaat
      421 gtaatattgt tgataaaaag gagaaaaaat gactaaaata tcttttgaaa tccaacaaca
      481 aattattcaa tgttttggat tatgttttca ctataaagat actgtcgttt catttatgca
      541 aacttcggga gttccaaatg atttaatatt aaaatggaaa tcggaaccga agtttgtttg
      601 ggcgaaaaat gttataaatg aacttaataa aacagaaaat ggaagattga taattagacg
      661 aatagcaact gaattttata agatgaaaaa tattcctgat gaagttcaag atagagatag
      721 aggattagat gcgttaagaa aattaaagcg actgataggt gatactcaac agaataaggt
      781 taacgaaaca cttaataatt cttatcatcg ttccagacag gaaatgaaaa tacaactcag
      841 gcaacaactc cttcaaaaaa ttgaagaact aaagacggag tattattcgc tattttcaag
      901 tgataatcct caagaaagag gatatcgttt agaaaaaatt gttgctaatt tattcagaat
      961 taatgatatt gattaccatg attcgtatag aaatagaaca aatactcaac agttagatgg
     1021 ctattttagg tttgaaggtt ttgattattt agttgaaatg aaatgggaaa aaaatccagt
     1081 aaattctccg aaaatagctt ctttaaaaca aaaagttgat acaaaactaa cttcgactcg
     1141 gggactgttt ctttcaataa atggttttag agatgaagta atacaagatt tttctaataa
     1201 agatgctaaa attttattta tggatggaca agaattagct tatattctag aaaatagaat
     1261 tagtttgtac gaagctttaa aagttaagat aattggtgct tctaaaactg gcaatccaaa
     1321 tgtttctata attaatcaag aataactttt ccgagagata ttaaaaccat caataaaata
     1381 gagcgatact ttatatgcca tgatacaaat gatatacaat gatacttctg accgttcagg
     1441 ctgccatcgt aaaagagcag caagtgaaat tcttatgatg acttcatcag tcatgccacg
     1501 ttaaatgtgt gagtttgtta gagaaacgca attaatcctc aaaaggttcc ccgaaccttt
     1561 tgagttccac agacgcatca cgtggagtgt gtaagcttgt tgctaaaagc gtaaaaacct
     1621 tggaacgaaa ggaataatag acttcctgcg aaacaaaaat acaatacaat aaaactatgg
     1681 atgatgaagc aagtaaacaa ttaagcgata gccgtttcaa gagccttgta ggtgttcagc
     1741 gcacgagatg ttagctgtgt taaaaacagc ttatcaacgc aacgtaaacg cgcaaaaggt
     1801 ggacgaaaaa ccaaattaag cctagacgat ctcctcatgg taactattca atacatgcga
     1861 gaatagcaca cttatgaaca aattgcggct gattttggca ttcacgaaag caacataatt
     1921 agtcggagtc aatggattga agcaactctt attcaaaatg gttttacgat ttcaaattct
     1981 gccttaattc tgtaaaaaca gtaaaattcg aaggattgta aggtaagagt ttttttcttt
     2041 ctgaaaaaat ggtataatag caatcaaaac tagaaaataa aacggaattt ggaacagatt
     2101 tgtctgtatc ctagtagagt ggtgatacta tgaagattag taagaggcac ttattaaatt
     2161 attccatctt gattccctac ttgcttttat ctattttggg cttgattgtg gtctattcga
     2221 ccaccagtgc tattttaatt gaagaaggca agagcgcctt gcagttggtt cgaaaccaag
     2281 gaatcttttg gattgttagt ttgatactga ttgccttaat ttataaattg agactagatt
     2341 ttttgagaaa tgagcgacta atcattttag ttatattaat agaaatgctt ttattgttct
     2401 tggctcgttt tattggtatt tccgtaaacg gggcatacgg ttggatttcg gttgcaggaa
     2461 taactattca gccagctgag tacttaaaaa tcattattat ttggtattta gctcaccgat
     2521 tctccaaaca gcaagaagaa atagctactt atgattttca agttttgact caaaatcaat
     2581 ggcttccccg tgcttttaat gattggcgat tcgttctcct agttctgatt ggaagtttgg
     2641 gaattttccc tgatttagga aatgcgacta ttttagtctt ggtttccttg attatgtata
     2701 cagttagtgg aatcgcttat cgctggtttt caaccattct ggcgctcgta tctgccgctt
     2761 ctgtctttgt cttgaccact atcagcctaa tcggtgttga gaccttttca aaaattccag
     2821 tatttggcta tgtagccaag cgctttagtg ccttttttaa tccttttgcc gatcgtgctg
     2881 atgcaggtca ccagttagct aattcttatt ttgccatggt caatggcggt tggtttggtc
     2941 taggtcttgg aaactcgatt gaaaaacgag gttatttgcc agaagctcat acagactttg
     3001 tcttttctat cgtgattgaa gaatttggct ttgttggtgc cagtcttatt ttagctctct
     3061 tatttttcat gattttgcgg attatcttgg tcggtatccg agcggagaat cctttcaatg
     3121 ccatggttgc actcggtgtc ggagggatga tgttggttca ggtatttgtc aatatcggag
     3181 ggatttcggg cttgattcca tctacaggag taaccttccc cttcttatcc cagggtggaa
     3241 atagtcttct agtcttatca gtggcagtag cctttgtctt aaatattgat gccagtgaaa
     3301 aacgcgctaa attgtaccga gaattggaaa atcaaccaat gaaccttctg ttgaagtagg
     3361 ataaagaaag gatagtttat gtctcttcaa aaattagaaa attatagtaa taaaagtgtt
     3421 gtgcaagaag aagtcttgat tctaacagaa ttactggaag atattactaa aaatatgctt
     3481 gccccagaga cctttgaaaa aataatacag ttgaaagaat tatcaacgca ggaagattat
     3541 caaggtctaa accgtctagt gactagctta tcaaatgatg aaatggtcta tatttcacgc
     3601 tatttctcta tcttgcctct tttgattaat atttcagagg atgtggattt agcttatgaa
     3661 atcaatcatc aaaataatat tgatcaggac tatttaggta aattatctac aacgattaaa
     3721 ttggtagcag aaaaggaaaa tgccgttgag atcctagaac acttgaatgt tgtccctgtt
     3781 ttgacagccc atccaacaca agtgcaacgc aaaagtatgt tggatttaac aaatcatatt
     3841 catagtcttt tgcgtaaata ccgtgatgtt aagttggggt tgattaataa agataaatgg
     3901 tacaatgatt tgcgtcgtta catcgaaatt atcatgcaga cagacatgat tcgtgagaaa
     3961 aaattaaaag tgactaacga aatcacgaat gctatggaat attataacag ctcctttttg
     4021 aaagctgtac ctcatttgac gacggagtat aagcgcttag cgcaagcgca tggtctgaat
     4081 ttaaaacagg ctaaaccaat caccatgggt atgtggatag gtggtgaccg tgatggaaat
     4141 ccatttgtta cagcaaagac cttgaagcag tctgcactca ctcagtgtga agtcatcatg
     4201 aactactatg ataaaaagat ttaccaactt tatcgtgaat tttctctttc aactagcatt
     4261 gtcaacgtca gcaagcaagt cagagaaatg gctcgtcaat ccaaggataa ctcgatttac
     4321 cgcgaaaaag agctttaccg tcgtgccttg tttgatattc aatcaaaaat tcaggcaact
     4381 aaaacctatc tgattgagga tgaagaagtt gggactcgtt atgaaaccgc caatgatttc
     4441 tacaaggatt tgattgccat tcgagattct ctactagaaa ataagggtga gtccttgatt
     4501 tcaggtgatt ttgtggaatt attgcaggca gtagagatat ttggttttta cttagcgtca
     4561 attgatatgc gacaagactc tagcgtctat gaagcctgtg tggcagaact cttgaaatca
     4621 gcaggaattc attctcgtta tagcgagttg agcgaagaag aaaagtgtga ccttctcttg
     4681 aaagaattag aagaagatcc ccgaattctt tctgcgactc acgcagaaaa atcagaatta
     4741 ttagcaaaag aattagctat ttttaagacg gctcgtgttt tgaaagataa gttgggagat
     4801 gatgtcatcc gtcagaccat catttcacat gcaaccagcc tttctgatat gctagaatta
     4861 gctattctgt taaaagaagt aggactggtg gatacggaaa gggcgcgtgt tcagattgtt
     4921 cccctttttg aaacaattga agacttggat cattcagagg aaacaatgag aaaatatctt
     4981 tctcttagcc ttgccaaaaa atggattgac tcacgaaata actaccaaga aatcatgctt
     5041 ggctactctg acagtaataa agatggcggt tacctgtcat catgttggac cctctacaag
     5101 gctcaacaac aattgactgc tattggagat gaatttggcg ttaaggttac cttcttccat
     5161 ggtcgtggtg gtactgtcgg tcgtggtggt gggccaacct atgaagccat tacatctcaa
     5221 ccgctcaagt ctatcaagga tcgtatccgc ttgacggagc agggtgaagt aattgggaat
     5281 aaatacggta acaaagacgc cgcttactat aaccttgaaa tgctagtatc ggcagctatt
     5341 aaccgtatga ttactcagaa gaagagcgat accaataccc caaatcgtta tgaagccatt
     5401 atggatcaag tagtggaccg tagttacgat atctaccgtg atttggtctt tggtaatgag
     5461 catttctatg attatttctt cgagtcaagt ccaatcaagg ctatttcaag ttttaatatt
     5521 ggttctcgtc cagccgctcg taagactatt actgaaatcg gtggtttgcg tgccatccct
     5581 tgggtattct catggtcaca gagtcgtgtt atgttccctg gatggtacgg ggttggttca
     5641 agcttcaagg aatttatcaa taaaaatcca gagaatattg ctatcttacg agatatgtac
     5701 caaaattggc ctttcttcca atcgcttctt tcaaatgttg atatggtttt gtcaaaatca
     5761 aatatgaata ttgcttttga atatgctaaa ctttgtgaag acgagcaagt taaggccatc
     5821 tatgagacta ttttaaatga atggcaagtt actaagaacg ttatcttggc tattgaagga
     5881 catgacgaac tcttagctga caatccatat ctaaaagcta gtctggatta ccgtatgcct
     5941 tactttaata ttctcaacta tattcagtta gagttgatta aacgccaacg tcgtggagaa
     6001 ttgtccagtg atcaagaacg attgattcat atcaccatca acggaattgc gacaggattg
     6061 cgtaattcag gttgataatt ttcaagagtg aatgctaaaa gtgaatatca aaaaaattct
     6121 aatagactat tgacaagtag tttaaaaatg atataattta accattcaga aaagtaatca
     6181 tacaaacttt ttagagagtc tgtggtagct gaaaacagat aagtggcgat gatgaaaatt
     6241 gggctgaatg ctatttagaa tttgaaatta taaaaattcg gtaagcacac cttacagtgc
     6301 atctcgttat tgcgagactg agcgataggg aaattcccta taattgaggt ggtaccgcgc
     6361 atcgacgtcc tcacacaagt tttttgtgtg aggatttttt tgatggaggt tagtatggaa
     6421 agaaaacgat ggcgtcgctt gtttagataa gtgaaatatg ttaaaggaaa taaaaaggag
     6481 aaacagaatg aaaaataaac gtttaattgg aattattgct gcattagcag tcttagtagc
     6541 aggaaacttg atttattctt caatgaataa atcagaagtt cagaataata aggatgagaa
     6601 gaaaataacc aagattggtg tgcttcaatt tgtgagccat ccatcccttg atttgattta
     6661 taaagggatc caagatggac ttgcagaaga aggatataaa gatgatcaag ttaaaattga
     6721 ttttatgaac tcagaaggtg accaaagtaa ggttgcgaca atgagtaaac aattggttgc
     6781 aaatggaaat gaccttgtgg ttggtatcgc aacaccagca gcccaagggt tggctagtgc
     6841 aacaaaagac ctaccggtta tcatggccgc tattacagac ccaattggtg ctaacttggt
     6901 taaagatttg aaaaaaccag gtggcaacgt tacaggggta tctgaccaca atccagctca
     6961 acaacaagtt gaactcatca aggctctgac accgaatgtg aaaacaatcg gagctcttta
     7021 ctcaagtagc gaagacaatt caaaaacaca ggtcgaagaa tttaaggctt atgctgaaaa
     7081 agcaggtctg acagtggaaa catttgcagt tccttcaaca aatgaaattg cctcaactgt
     7141 cactgttatg actagcaagg tagatgctat ttgggttcca attgataaca ccattgcatc
     7201 aggatttcca acggttgtct ctagcaatca aagttctaag aaaccaattt atcccagtgc
     7261 gacagctatg gtagaagtag gtggtttggc atcagttgta attgaccaac atgaccttgg
     7321 tgtggcaaca ggtaaaatga ttgtgcaagt cttgaaaggt gcaaaaccag ccgatacccc
     7381 agtcaatgtc ttttcaattg gtaagtcagt catcaataaa aaaatagcac aagaactagg
     7441 tattactatt cctgagtctg ttctcaaaga agcaggacaa gtcatcgaat aaataaggta
     7501 gggaggagga gactcttccc attttttaaa aagatgaata tagaaaggat taaataaaat
     7561 atgattgttt ccattatttc tcaaggattt gtctgggcta ttctaggtct gggaatcttt
     7621 atgacattta ggattttaaa ctttccagat atgacgacag aaggttcctt ccctcttggg
     7681 ggagctgttg ctgtcacttt gataaccaaa ggcgtgaacc catttttagc gacacttgtt
     7741 gctgtaggag caggttgttt ggctggaatg gcagcaggcc ttctttatac aaaagggaag
     7801 atcccaacct tgctctcagg gattttggtg atgacttctt gtcactcaat catgctcttg
     7861 attatgggac gtgcgaattt aggcctgctt ggaaccaagc aaattcagga tgttttgcct
     7921 tttgattcgg atttgaatca actcttgaca ggtctcatct ttgtgagcat tgttattgct
     7981 ctcatgctct ttttcttgga cactaaactc ggacaagcct atattgctac aggggataat
     8041 cctgatatgg ctagaagttt cgggattcat actggacgca tggagctcat gggcttggtc
     8101 ttatcaaatg gtgtgattgc ccttgcaggt gccctcattg ctcagcaaga aggttatgcc
     8161 gatgtgtctc gagggatcgg ggttatcgtt gtggggcttg caagtttgat tattggagaa
     8221 gttattttca agagtttgag cttggcagag cgtttggtta ctatcgttgt aggttctatc
     8281 gcttatcaat ttttagtgtg ggcagttatc gcacttggct ttaatacaag ttaccttcgt
     8341 ttatacagtg ccttgatttt agcagtctgc ctcatgattc caacatttaa gcaaacaatc
     8401 ttgaaaggag ccaagttaag caaatgacag caattgtaga attaaaaaat gcaaccaaaa
     8461 tcgttaaaaa tggctttgat gaagaaaaga ttattttaaa tgatgtttcc ttagaaattt
     8521 ttgaacggga ctttatcacg attttgggcg gaaatggtgc tggaaaatca accctcttta
     8581 acactatagc agggacctta tcactaacta gtggaactat ccgtatttta ggtgaagatc
     8641 tcactaagtt ttcacccgag aagcgtgcca agtacctgtc tcgtgtcttc caagatccaa
     8701 agatggggac agctccccgt atgacggtcg ctgaaaatct tttaatcgcc aagtttcgtg
     8761 gtgaaaagcg tggattgtta ccaagacgct tgactagcta taaggatgaa tttcaggcaa
     8821 ccattgaaaa agtaggaaat ggtcttgaga aacacttgaa tacaccgatt gagttcttat
     8881 caggtggaca aagacaggct ttgagtctct tgatggcaac cttgaagcga cctgaattac
     8941 tcttgttaga cgagcatact gctgccctgg atccaaagac tagtgttgct ttgatggaat
     9001 tgacagatga atttgttaag aaagatcagc taacagccct tatgattact catcatatgg
     9061 aagatgctct caaatacggc aatcgcttga ttgtcatgaa agaaggacgg attatccaag
     9121 atttaaacca agaagaaaaa gcaaaaatga aaatctctga ttattatcaa ctctttgaat
     9181 aaagtaagga aaatgagtga aatggtttac tttttttctg aaataaagta tactatataa
     9241 agtaaactat gataacatgg aggtattgtg tatggttaac aaacaagtca ttgaagaaat
     9301 caaaaacaat gccaacattg tggaagtcat aggagatgtg atttctttac aaaaggcagg
     9361 acggaactat ctagggctct gtccttttca tggtgaaaaa acaccttctt tcaacgttgt
     9421 agaggataag cagttttatc actgtttcgg ttgtggtcgc tcaggtgatg tctttaagtt
     9481 catagaggag taccaagggg ttccctttat agaggcagtc caaatcttag gtcagcgtgt
     9541 tggaattgag gttgaaaaac cgctttatag tgagcaaaag tcagcctcac ctcaccaaga
     9601 tctttatgat atgcacgaag atgcagccaa attttaccat gctattctca tgacaacgac
     9661 tatgggcgaa gaggccagaa attaccttta tcagcggggt ttgacagatg aagtgcttaa
     9721 acatttttgg attggtttag cacctccaga acgaaactat ctctatcaac gtttgtctga
     9781 tcagtatcgt gaagaggatt tactggattc aggcctgttt tatctctcgg atgccaatca
     9841 atttgtagac acctttcaca atcgcattat gtttcccctg acaaatgacc aaggaaaggt
     9901 cattgccttc tcaggtcgta tctggcaaaa aacggattca caaacttcta agtataaaaa
     9961 cagccgatcg actgcaattt ttaacaaaag ttacgaatta tatcatatgg atagggcaaa
    10021 aagatcttct ggaaaagcta gtgagattta cctgatggaa ggattcatgg atgttattgc
    10081 agcctatcgg gctggaatcg aaaatgctgt ggcgtcgatg ggaacggcct tgagtcgaga
    10141 gcatgttgag catctgaaaa ggttaaccaa gaaattggtt cttgtttacg atggagataa
    10201 ggctgggcaa gccgcgacat tgaaagcatt ggatgaaatt ggtgatatgc ctgtgcaaat
    10261 cgttagcatg cctgataact tggatcctga tgagtatcta caaaaaaatg gtccagaaga
    10321 cttggcctat ctattaacga aaactcgtat tagtccgatt gagttctaca ttcatcagta
    10381 caaacctgaa aacagtgaaa atctgcaggc tcagattgag tttcttgaaa aaatagctcc
    10441 cttgattgtt caagaaaagt ccatcgctgc tcaaaacagc tatattcata ttttagctga
    10501 cagtctggcg tcctttgatt atacccagat tgagcagatt gttaatgaga gtcgtcaggt
    10561 gcaaaggcag aatcgcatgg aaagaatttc cagaccgacg ccaatcacca tgcctgtcac
    10621 caagcagtta tcggctatta tgagggcaga agcccatcta ctctatcgga tgatggaatc
    10681 ccctcttgtt ttgaacgatt accgtttgcg agaagacttt gcatttgcta cacctgaatt
    10741 tcaggtctta tatgacttgc ttggccagta tggaaatctt cctccagaag ttttagcaga
    10801 gcagacagag gaagttgaaa gagcttggta ccaagtttta gctcaggatt tgcctgctga
    10861 gatatcgccg caggaactta gtgaagtaga gatgactcga aacaaggctc tcttgaatca
    10921 ggacaatatg agaatcaaaa agaaggtgca ggaagctagc catgtaggag atacagatac
    10981 agccctagaa gaattggaac gtttaatttc ccaaaagaga agaatggagt aataatggca
    11041 acaaaacaaa aagaagtaac aacatttgac gtacaggtag cagaatttat ccgtaatcat
    11101 aagcaaaaag ggacagcaac agatgatgaa atcaatgcaa gtctggttat tccttttacc
    11161 ttggacgctg atgggattga agatctcttg caacggattc aggatgcagg gatttctatc
    11221 acagataacg aaggaaatcc aagtgcgcgt gttcttagca atgaagaaga accagaactc
    11281 agcgatgagg acttgattgg atcaacttct gctaaggtca atgaccctgt ccgtatgtac
    11341 ttgaaagaaa taggggtcgt tcctctcttg accaatgaag aggagaaaga gttggcactg
    11401 gctgttgaag ctggcgatat cgaagccaaa caacgtcttg cggaagccaa tcttcgtttg
    11461 gttgtttcca ttgccaaacg ctatgtcggt cgtggcatgc agttccttga cttgattcaa
    11521 gaaggaaata tgggcttgat gaaggcggtt gacaagtttg actattctaa agggttcaag
    11581 ttttcaactt atgcaacttg gtggattcgt caggctatca ctcgtgctat tgcggaccaa
    11641 gctcgtacca tccgtatccc agttcacatg gttgaaacta tcaataaatt ggttcgtgaa
    11701 cagcggaatc tccttcaaga attggggcaa gatccgacac cagaacagat tgctgaacga
    11761 atggatatga cacctgataa ggttcgtgaa atcttgaaga ttgcccaaga accagtatct
    11821 cttgaaactc ctatcggtga agaggacgat agccaccttg gagactttat cgaagatgaa
    11881 gtgattgaaa atccagtgga ttatacgact cgtatcgtct tgcgtgagca attggatgaa
    11941 atcttagata ctcttacaga ccgtgaagaa aatgttctgc gtctacgttt tggactagat
    12001 gatggaaaaa tgcgcacact tgaagatgtg gggaaagtct ttaacgtaac tcgtgagcgt
    12061 atccgtcaga ttgaagcaaa ggctttgaga aaactacgcc aaccaagccg cagcaaaccg
    12121 cttcgtgatt ttattgaaga ctaagagtga ggaaaatatg gcttatacag aagagcaaat
    12181 tgaaaacatc aaaacacgga ttttaacagc cttggaagaa gtcatcgacc ctgagttggg
    12241 aatcgatatt gtcaatcttg gtttgatcta tgagattcgt tttgacggtg acacagggca
    12301 aacagagatt gacatgactt tgacaactat gggttgtccc ttagcagacc ttttgacaga
    12361 ccagatttat gatgctatga tagaggttcc agaagtaacg gatactgagg ttaaattagt
    12421 ctggtatcca gcctggacag ttgaaaaaat gagtcgctat gcccgcattg ccctaggcat
    12481 taagtaaaca tataaaagca tgtgagagac tgttggaaaa gcgaggaaat ttcccctctt
    12541 ttcctctagt ctctcctttc ttttgctgat tttattcaaa gaaaatgata taatagtagt
    12601 tatggagaaa aagaaattac gcatcaatat gttgagttca agtgagaaag tagcaggaca
    12661 gggagtttca ggtgcttacc gtgaattagt tcgtcttctt caccgtgctg ccaaggacca
    12721 attgattgtt acagaaaatc ttccgatcga ggcagatgtg actcactttc atacgattga
    12781 ttttccctat tatttatcaa ccttccaaaa gaaacgctca gggagaaaga ttggctatgt
    12841 gcatttcttg ccagctacac ttgagggaag tttgaaaatt ccatttttct taaaggaaat
    12901 tgtgaaacgc tatgtatttt ctttttacaa ccggatggag cacttggttg tggtcaatcc
    12961 tatgtttatt gaggatttgg tagcagctgg tattccacgt gaaaaagtga cctatattcc
    13021 taactttgtc aacaaggaaa aatggcatcc tctaccacaa gaagaggtag ttagactgcg
    13081 cacagatctt ggtcttagtg acaatcagtt tatcgtagta ggtgctgggc aagttcagaa
    13141 acgtaaaggg attgatgact ttatccgtct ggctgaggaa ttgcctcaga ttacctttat
    13201 ctgggctggt ggcttctctt ttggtggtat gacagatggt tatgaacact ataagaaaat
    13261 tatggaaaat ccccctaaaa atttgatttt tccaggcatt gtatcgccag agcggatgcg
    13321 cgaattgtat gctctagcgg atcttttctt gttgcctagt tacaatgagc tctttcctat
    13381 gactatttta gaagctgcga gttgtgaggc tcctattatg ttgcgtgatt tagatctcta
    13441 taaggtgatt ttggagggaa attatcgggc gacagcgggt agagaagaga tgaaagaggc
    13501 tattttggaa tatcaagcaa atcctgctgc cttaaaagat ctcaaagaaa aggctaagaa
    13561 tatttccaga gagtattctg aagagcatct gttacaaatc tggttggact tttatgagaa
    13621 acaagccgct ttagggagaa agtaaaaagt gaggtaatct atgcgaattg gtttatttac
    13681 agatacctat tttcctcagg tttctggtgt tgcgaccagt attcgaacct tgaaaacaga
    13741 acttgaaaag cagggacatg ctgtttttat ctttacgacg acagataagg atgtcaatcg
    13801 ctacgaagat tggcaaatta tccgcattcc aagtgttcct ttctttgctt ttaaggatcg
    13861 tcgctttgcc taccgaggtt ttagcaaggc acttgaaatt gctaaacagt atcagctaga
    13921 tattatccat actcagacag aattttctct tggcctgttg gggatttgga ttgcgcgtga
    13981 attgaaaatt ccagtcatcc atacctatca cacccagtat gaagactatg tccattatat
    14041 tgctaagggg atgttgatcc ggccgagtat ggtcaagtat ctggttagag gtttcctgca
    14101 tgatgtggat ggggttattt gtcctagtga gattgtccgt gacttgctat ctgattataa
    14161 ggtcaaggtt gaaaaacggg tcattcctac tgggattgaa ttagccaagt ttgagcgtcc
    14221 ggaaatcaag caggaaaatt tgaaagaact gcgtagtaaa ctagggattc aagatggtga
    14281 aaagacgttg cttagtcttt cgagaatctc ctatgaaaaa aatattcaag cagttttagc
    14341 agccttttct gatgttctga aagaggaaga caaggttaaa ctggtagtag ctggggatgg
    14401 cccttatctg aatgacctca aagagcaagc tcagaaccta gagattcaag actcagtcat
    14461 ctttacaggg atgattgctc ctagtgagac ggctctttac tataaagcgg cggatttctt
    14521 catttcggca tcgacaagcg aaacgcaagg tttgacctac ttggaaagct tagccagtgg
    14581 aacacctgtc attgctcacg gaaatcctta tttgaacaac ctcatcagtg ataaaatgtt
    14641 tggaaccttg tactatggag aacatgattt ggctggtgct attttggaag ccctgattgc
    14701 aacaccagac gtgaacgagc ataccttatc agagaaattg tatgagattt cagctgagaa
    14761 ctttgggaaa cgagtgcatg agttttatct ggatgccatt atttcaaata acttccagaa
    14821 agatttggct aaagatgata cggtcagtca gcgtatcttt aagacagttt tgtatcttca
    14881 gcaacaggtg gttgctgtac ctgtaaaagg atctagacgc atgttgaagg cttcaaaaac
    14941 acagttgatc agtatgagag actattggaa agaccatgaa gaatagaaag aggaacagct
    15001 atgaaaaaaa caattaatga gaagcggtcg tgataaaaag attgcgggtg tttgtgctgg
    15061 ggtggcccat tatctggata tggatccgac tatcgttcaa gtcatttggg gtgttcttac
    15121 ttgctgttac ggagctggaa ttgtagctta cattatttta tggattatcg caccagtagc
    15181 aactgactat tgaaaactag ctcaattcat gctaagataa tagaaaatag aatgtaacga
    15241 aggagagaaa aatggcattt ggagataatg gaaatcgtaa aaaaactatg tttgagaaaa
    15301 taaccttgtt tatcgtgatt atcatgctag tagcaagttt attgggaatt tttgcaactg
    15361 caattggtgc cctcagtaat ctataaaata gattcaagaa aatttagtga ctgggatttc
    15421 ccagcccttt tttaaagtga gaagaaataa tgagtatgtt tttagataca gctaagatta
    15481 aggtcaaggc tggtaatggt ggcgatggta tggttgcctt tcgtcgtgaa aaatatgtcc
    15541 ctaatggagg cccttggggt ggtgatggtg gtcgtggagg caatgtggtc ttcgttgtag
    15601 acgaaggact acgtaccttg atggatttcc gctacaatcg tcatttcaag gctgattctg
    15661 gtgaaaaagg gatgaccaaa gggatgcatg gtcgtggtgc tgaggacctt agagttcgag
    15721 taccacaagg tacgactgtt cgtgatgcgg agactggcaa ggttttaaca gatttgattg
    15781 aacatgggca agaatttatc gttgcccacg gtggtcgtgg tggacgtgga aatattcgtt
    15841 tcgcgacacc aaaaaatcct gcaccggaaa tctctgaaaa tggagaacca ggtcaggaac
    15901 gtgagttaca attggaacta aaaatcttgg cagatgtcgg tttagtagga ttcccatctg
    15961 tagggaagtc aacactttta agtgttatta cctcagctaa gcctaaaatt ggtgcctacc
    16021 actttaccac tattgtacca aatttaggta tggttcgcac ccaatcaggt gaatcctttg
    16081 cagtagccga cttgccaggt ttgattgaag gggctagtca aggtgttggt ttgggaactc
    16141 agttcctccg tcatatcgag cgtacacgtg ttatccttca catcattgat atgtcagcta
    16201 gcgaaggccg tgatccatat gaggattacc tagctatcaa taaagagctg gagtcttaca
    16261 atcttcgcct catggagcgt ccacagatta ttgtagctaa taagatggac atgcctgaga
    16321 gtcaggaaaa tcttgaagaa tttaagaaaa aattggctga aaattatgat gaatttgaag
    16381 agttaccagc tatcttccca atttctggat tgaccaagca aggtctggca acacttttag
    16441 atgctacagc tgaattgtta gacaagacac cagaattttt gctctacgac gagtccgata
    16501 tggaagaaga agcttactat ggatttgacg aagaagaaaa agcctttgaa attagtcgtg
    16561 atgacgatgc gacatgggta ctttctggtg aaaaactcat gaaactcttt aatatgacca
    16621 actttgatcg tgatgaatct gtcatgaaat ttgcccgtca gcttcgtggt atgggggttg
    16681 atgaagccct tcgtgcgcgt ggagctaaag atggggattt ggtccgcatt ggtaaatttg
    16741 agtttgaatt tgtagactag gagactggta tgggagataa accgatatct ttccgagatg
    16801 cggatggtaa ttttgtttcc gccgcagacg tttggaatga aaagaaattg gaagaactat
    16861 ttaatcgtct caatccaaat cgtgccttga gattggcacg aactaaaaag gaaaatccat
    16921 ctcagtaaag aagctaaaaa atcccgtgcc tcatcagaca cgggattttg tggtacgacg
    16981 ggcatgtata gcgaactgaa tctggaatag cacagcatat cttctaaaat atagtaaaat
    17041 gaaatgagaa caggacaaat cgatcaggac agtaaaatcg a
//
LOCUS       DAVLVZ010000038        16485 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000038 DAVLVZ010000000
VERSION     DAVLVZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.038"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            134..2299
                     /locus_tag="VYN77_001329"
     CDS             134..2299
                     /locus_tag="VYN77_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1558782.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKINKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKNGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2299..3039
                     /locus_tag="VYN77_001330"
     CDS             2299..3039
                     /locus_tag="VYN77_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1558783.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3188..4675
                     /locus_tag="VYN77_001331"
     CDS             3188..4675
                     /locus_tag="VYN77_001331"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1558784.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(4721..6010)
                     /gene="ftsY"
                     /locus_tag="VYN77_001332"
     CDS             complement(4721..6010)
                     /gene="ftsY"
                     /locus_tag="VYN77_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1558785.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEYIDPTFTEVEEVS
                     QEEAEVEIIEQAVFQEEEIQDTVEESLDSEPVVGVSQEEVEEFPHSEELPEEEKLEHE
                     GTVEESNSEVLEPEAPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDVLRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(6014..6832)
                     /locus_tag="VYN77_001333"
     CDS             complement(6014..6832)
                     /locus_tag="VYN77_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1558786.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSNQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6832..7626)
                     /locus_tag="VYN77_001334"
     CDS             complement(6832..7626)
                     /locus_tag="VYN77_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1558787.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSHDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            complement(7623..11162)
                     /gene="smc"
                     /locus_tag="VYN77_001335"
     CDS             complement(7623..11162)
                     /gene="smc"
                     /locus_tag="VYN77_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1558788.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAVLEDKRNS
                     LSQEKSDKESSLVLLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDVIAASAGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARKVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVTDIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11153..11851)
                     /locus_tag="VYN77_001336"
     CDS             complement(11153..11851)
                     /locus_tag="VYN77_001336"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1558789.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFNQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVIIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(12034..12105)
                     /locus_tag="VYN77_001337"
     tRNA            complement(12034..12105)
                     /locus_tag="VYN77_001337"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12070..12072),aa:Arg,seq:ccg)
     gene            complement(12169..13155)
                     /locus_tag="VYN77_001338"
     CDS             complement(12169..13155)
                     /locus_tag="VYN77_001338"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1558790.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENIAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(13330..14646)
                     /locus_tag="VYN77_001339"
     CDS             complement(13330..14646)
                     /locus_tag="VYN77_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1558791.1"
                     /translation="MRITDNQYKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKILHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(14633..16393)
                     /locus_tag="VYN77_001340"
     CDS             complement(14633..16393)
                     /locus_tag="VYN77_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV1558792.1"
                     /translation="MSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIY
                     WNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIG
                     RSAMVTKLLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYP
                     ELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGTGK
                     TYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKD
                     FCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSG
                     VPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYISPEEVETDKKF
                     VFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIG
                     TMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIEN
                     VQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAF
                     ELTNNDQTGQQDTGDNDADY"
BASE COUNT         4790 a         3627 c         2917 g         5151 t
ORIGIN      
        1 gcaattttta atagcaattc aagaaagaaa aggaaaaatc aatgttatat tttctgacac
       61 gaaaaatata aaaatttgcc tttttttact ttttctgata taatgagaga atattcggaa
      121 aaggagacta aaaatgaaga aaaaatttct agcatttttg ctaattttat tcccaatttt
      181 ctcattaggt attgccaaag cagaaacgat taagattgtt tctgataccg cctatgcacc
      241 ttttgagttt aaagattcag atcaaactta taaaggaatt gatgttgaca ttattaacaa
      301 agtcgctgag attaaaggct ggaacattca gatgtcctat cctggatttg acgcagcagt
      361 caatgcggtt caagctgggc aagccgacgc tatcatggca gggatgacaa agactaaaga
      421 acgtgaaaaa gtcttcacca tgtctgatac ttactatgat acaaaagttg tcattgctac
      481 tacaaagtca cacaaaatta acaagtacga ccaattaact ggcaaaaccg ttggtgttaa
      541 aaacggaact gccgctcaac gtttccttga aacaatcaaa gataaatacg gttttactat
      601 taaaacattt gacactgggg atttaatgaa caacagcttg agtgctggtg ccatcgatgc
      661 catgatggat gacaaacctg ttatcgaata tgccattaac caaggtcaag acctccatat
      721 tgaaatggat ggtgaagctg taggaagttt tgctttcggt gtgaaaaaag gaagtaaata
      781 cgaacacctg gttactgaat ttaaccaagc cttggctgaa atgaaaaaaa atggtagtct
      841 tgataaaatt atcaagaaat ggactgcttc atcatcttca gcagtgccaa ctacaaccac
      901 tctcgcagga ttaaaagcta ttcctgttaa ggctaaatat atcattgcca gcgattcttc
      961 ttttgcacct tttgttttcc aaaattcaag caaccaatac actggtattg atatggaatt
     1021 gattaaggca atcgctaaag accaaggttt tgaaattgaa atcaccaacc ctggttttga
     1081 tgctgctatc agtgctgtcc aagctggtca agccgatggt atcattgctg gtatgtctgt
     1141 cacagatgct cgtaaggcaa cttttgactt ctcagaatca tactacactg ctaataccat
     1201 tcttggtgtc aaagaatcaa gcaatattgc ttcttatgaa gatctaaaag gaaagacagt
     1261 cggtgttaaa aacggaactg cttctcaaac cttcctaaca gaaaatcaaa gcaaatacgg
     1321 ttacaaaatc aaaacctttg ctgatggttc ttcaatgtat gacagtttaa acactggtgc
     1381 cattgatgcc gttatggatg atgaacctgt tctcaaatat tctatcagcc aaggtcaaaa
     1441 attgaaaact ccaatctctg gaactccaat cggtgaaaca gcctttgccg ttaaaaaagg
     1501 agcaaatcca gaactgattg aaatgttcaa caacggactt gcaaacctta aagcaaacgg
     1561 tgaattccaa aagattcttg acaaatacct agctagcgaa tcttcaactg cttcaacaag
     1621 tactgttgac gaaacaacgc tctggggctt gcttcaaaac aactacaaac aactccttag
     1681 cggtcttggt atcactcttg ctctagctct tatctcattt gctattgcca ttgtcatcgg
     1741 aattatcttc ggtatgttta gcgttagccc atacaaatct cttcgcgtca tctctgagat
     1801 tttcgttgac gttattcgtg gtattccatt gatgattctt gcagccttca tcttctgggg
     1861 aattccaaac ttcatcgagt ctatcacagg ccaacaaagc ccaattaacg actttgtagc
     1921 tggaacaatt gccctctcac tcaatgcggc tgcttatatc gctgaaatcg ttcgtggtgg
     1981 tattcaggcc gttccagttg gccaaatgga agccagccga agcttgggta tctcttatgg
     2041 aaaaaccatg cgtaagatta tcttgccaca agcaactaaa ttgatgttgc caaactttgt
     2101 caaccaattc gttatcgctc ttaaagatac aactatcgta tctgctatcg gtttggttga
     2161 actcttccaa actggtaaga ttatcattgc tcgtaactac caaagtttca agatgtatgc
     2221 aatccttgct atcttctatc ttgtaattat cacacttttg actagactag cgaaacgctt
     2281 agaaaagagg attcgttaat ggcaaaatta aaaattgatg taaatgattt acacaagcac
     2341 tatggaaaaa atgaagtcct aaaaggaatt acgactaagt tctatgaagg agatgttgtt
     2401 tgtatcatcg gtccttcagg ttctggtaag tcaactttcc tccgtagcct caatctttta
     2461 gaagaagtca ctagcggtca catcactgtg aacggctatg atttaactga aaaaacaacc
     2521 aatgttgacc acgtccgtga aaatatcggc atggtattcc aacacttcaa cctcttccct
     2581 catatgtctg tattggacaa catcaccttt gctcctattg agcacaagtt gatgactaag
     2641 gaagaagctg aggaattggg aatggagttg cttgaaaagg ttggactagc agataaagct
     2701 aatgccaatc cagatagcct atcaggtggt caaaaacaac gtgtggccat cgctcgtggc
     2761 ctagcaatga atccagacat catgctcttc gatgaaccaa cttctgccct tgaccctgag
     2821 atggttggag acgtacttaa cgttatgaag gaattggctg agcaaggcat gaccatgatt
     2881 atcgtaaccc atgagatggg atttgctcgt caggttgcca accgcgttat ctttactgca
     2941 gatggcgagt tccttgaaga cggaacacct gaccaaatct ttgataaccc acaacaccct
     3001 cgtctgaaag agttcttaga taaggtctta aacgtctaaa ctcaaactgt aaggatttcc
     3061 ttgcagtttt tctacctcgt attggaattt ttgatttttc ggaaaattat gttagaatta
     3121 agtttatgaa atgaggtttc ctcataccta gcaagactag gaataaaaat agaaattagg
     3181 tagctagatg tcatctaagg ttattgttac aattttcggt gcgagtggag acctggctaa
     3241 acgcaagctc tacccttccc tttttagact atataaatcc ggcaatcttt ccaagcactt
     3301 tgccgttatt ggaactgccc gtagaccttg gagtaaggaa tattttgaat ctgtagttgt
     3361 cgagtccatc cttgatttgg cagatagtac cgagcaagcc caagaatttg ctagccactt
     3421 ctactatcaa agccatgatg tcaatgattc ggaacattat attgctttgc gtcaattaca
     3481 agctgagctt aatgaaaaat accaagctga acacaataag ctcttcttct tgtctatggc
     3541 acctcagttc tttggaacca ttgccaaaca cctcaaatct gaaaacattg tcgatggcaa
     3601 aggttttgag cgcttgatcg ttgaaaaacc atttggtaca gattacgcaa ctgcaagcaa
     3661 gttgaatgac gaactcctag caacatttga cgaagaacaa attttccgta tcgaccatta
     3721 tcttggtaag gaaatgatcc aaagcatctt tgcagttcgc tttgcaaact tgatttttga
     3781 aaacgtttgg aacaaggatt ttatcgacaa tgttcaaatt acctttgcgg agcgcttggg
     3841 tgtagaagaa cgtggtggct actatgacca atccggtgcc ctccgtgaca tggtccaaaa
     3901 ccacactcta caacttcttt cgctcctcgc catggacaaa ccagcaagct tcacaaaaga
     3961 cgagattcgt gctgaaaaga ttaaggtctt taaaaacctc tatcatccaa ctgatgaaga
     4021 actcaaagaa cactttatcc gtgggcaata ccgctctggt aagattgatg gcatgaaata
     4081 catctcttat cgtagcgagc caaatgtgaa tccagaatca acaactgaaa cctttacatc
     4141 tggtgccttc tttgtagaca gcgatcgatt ccgtagtgtt cctttctttt tccgtacagg
     4201 taaacgactg actgaaaaag gaactcatgt caacatcgtc tttaaacaaa tggattctat
     4261 ctttggagaa ccacttgctc caaatatttt gaccatctat attcaaccaa cagaaggctt
     4321 ctctcttagc ctaaatggga agcaagtagg agaagaattt aacttggctc ctaactcact
     4381 tgattatcgt acagacgcga ctgcaactgg tgcttctcca gaaccatacg aaaaattgat
     4441 ttatgatgtc ctaaataaca actcaactaa ctttagccac tgggatgaag ttggtgcgtc
     4501 atggaagttg attgatcgta ttgaaaagct ctgggctgaa aatggtgccc cacttcatga
     4561 ctataaagct ggaagcatgg gacctcaagc cagctttgac ctacttgaaa aattcggtgc
     4621 caaatggact tggcaaccag atatcgccta tcgtcaagat ggtcgcttag aataaaaaaa
     4681 tttcctgcaa gtttatgcct tgcaggattt ttgcttctga ttagattaaa ccttccaaga
     4741 gacctttcat aaagttttct gagttaaact ctccaatatc atcgattttt tcaccaaaac
     4801 caatcaattt tacaggaata ttgagttctt cacgaatggc tagaaccaca cctcctcgag
     4861 cagttccatc aatcttagtc aaaacaattc cggttaaagg tgtgattttc gaaaattctt
     4921 tggcctgtac tagggcattt tgacctgttg atgcatcaag tgccaagaag gtttcatgtg
     4981 gtgcttctgg cacaacacgt ttgataatac gaccaatctt ttccaactca gccataaggt
     5041 tatccttatt ttgcagacga ccagcagtat caatcatgag aatatcgata ccttcagcca
     5101 cggcacgttc cataccatca aagaccacgc tggctggatc agctttttca ggtccagtta
     5161 ctactggaac atctactcgt cggccccatt cagctagctg agctactgca cccgcacgga
     5221 aggtatctgc tgcaaccagc atgaccttct taccagcttg tttgtagcgg tgggctagtt
     5281 ttccgataga agttgttttc ccaacaccat tcacaccaac aaagagcata actgtcaagt
     5341 tatcttggaa gtggatgctt tcatcgtagc taccatcctt ttcataaagc tcaaccaatt
     5401 tctcaatgat gacacgacga agtacatcag gtttcttggc attttcaagc ttggcttcgt
     5461 aacgtagttc ctccgttaag ttagaagcga cttggacacc aacatcactc ataatcagca
     5521 gttcttccag ttcctcgaaa aattcttcgt caacagagcg gaagttagca aagaaggcat
     5581 tcaagcgggc accgaaacct gtgcgagttt tcttaagact gcggtcatat ttttcctgaa
     5641 cagtttcttc tgtttgagga gcttctggtt caagcacttc agaattactt tcttctacag
     5701 ttccttcgtg ctcaagcttc tcttcctctg gtaattcttc tgagtgtgga aattcttcga
     5761 cttcttcttg agaaacccct acaactggct ctgaatccag actttcttca actgtgtctt
     5821 ggatttcctc ttcttggaac acagcttgtt caataatttc aacctctgct tcttcttgag
     5881 aaacttcctc aacttctgtg aaggtaggat caatatattc agacaaatca agattttcca
     5941 gagcttcttt tacaacttct tcgattttag gttcttcttt ttttccgaat agacggtcaa
     6001 ataatcccat atcttagttc tcctttagca catattcttc gatagcccag gcgacagctt
     6061 cttcatcatt ggtcatagga gttaccacat ttgcgactgc cttgacttca ggaacagcgt
     6121 tttgcatggc aacaccgagc cctgcccatt caatcataga gaggtcattg gcctcgtcac
     6181 cacaggccat cacttgactt tgatcaattc caagatggct aattagtttt gccaaacctg
     6241 ttgctttatg aacattcttt ggtgaccatt ctagcaacat ttcacgtgat ttaaagattt
     6301 catattggtc aaacaattct ggagaaatct tctgaatggc tgcatccaag ggttcttgag
     6361 caaaggcggt cacgcatttg ttgtaggtca tttgattaga caggtcttca aaatcaactg
     6421 gaacaaaagt caaagctgga ttgaatttgg cataaagact ttcttggtcc gattgaattt
     6481 gataaacagt tccttctgag atagcgtcaa gaggcagtga taatttctct gtttcttcat
     6541 acaagcgtgc cacatcatca tatgaaaaga ctgtcttatc aaggatttca cctgtatttt
     6601 tctgaaccaa accaccatta aaagtaatgg tatactcatc ttcctgaccg tcagtcccta
     6661 gctcatggag aaagaaatcc atagctttta agggacgacc agttgtcaat acgaccttga
     6721 taccacgatc acgcgcagct ttcaaggttt ccttggtacg atccgtcagc cttttatcag
     6781 tagtcagcaa ggtcccgtcc aagtccaatg cgattaattt tatatctgcc attataagcc
     6841 ctccatataa gctataaccg actggtcctt atggtgacca atcacagtct ttgctaattc
     6901 taaaatttca ggtcgtgcat tttcaggagc tacaggatgt cccacaacct gcatcatatg
     6961 taagtcatta agattgtctc caaaagccat gacctgatcc attgtgatac caagtttttt
     7021 aactaattca acaatggcca ctcccttatc gacatagtcc agaacaatat caatagattc
     7081 aaagccagtt gtcatagcct taacaccagg aacgttttcg tttacccaag cctccccatc
     7141 ttccagcgtt tcttctgtga agttggttgt aaatttgaaa atgtcatctg tgatatcttc
     7201 caaactcgct actttttgga tattttcatt ataatgctga ctcactttca aataggtctc
     7261 atcaaccgta tctagaacat atgaaccctt cttacccgtc aagagcagtt tattgatatc
     7321 tacataaggt gacgttttca gcttttcaaa agttgccaga taaaagtcat gagacatagt
     7381 cgcttcatac aagtcctgac cttgatactc taccaaactg ccattttccg cgatgaaaat
     7441 aatgtcatca cgaacatcag caaataattt ttccagagac agaaatcccc gacccgaagc
     7501 caccgcaaag taaatccctt tttccttgta ggaagccaag agagacttga gacgatccat
     7561 atcaaagcgt ccattcccat ctaggaaggt tccgtccata tccgttgcta ctagtttaat
     7621 tgtcatcctt caatactttc taaatctttt aacttaactg aaacaatttt tgagacacct
     7681 gattcttgca tggtcactcc atagatagaa tcagccgctg ccatggttcc cttacggtgg
     7741 gttacgacga tgaactggct gtccttgtca aagcggttga ggtaatcccc aaaacgttta
     7801 acattagctt catccagcgc agcttccacc tcatccaaga taacaaatgg aatagtcttg
     7861 acacgaataa tggagaagag caaggcaaga gccgatagag ctttttcacc accactcatg
     7921 aggttaagcg actggatttt cttacctgga ggttgaacag aaatctccac accagctgtt
     7981 aaaaggtcgc cctcagtcaa tatcaagtct gcctgacctc cgccaaacat ctgcttgaag
     8041 gtcactttaa aggactcacg aatagcttca aaggttgatt taaagcgttc cttgacctca
     8101 tcattcatct ctgtaatggt ttcaaggagc agatttttcg ctgacaaaat atcatctcgc
     8161 tgactattta gaaagtccag acggttgtga acttcttcgt actggtcaat agcttctata
     8221 ttgacaggac ccagtgagcg aatagccttc tctaaatcct taacttcttg ctctgccaga
     8281 ttgaggtttt ccaattcatg cgctttttct agagcttcag tatagctaat ctggtactgg
     8341 tctgttaatt gactttgtag atggcgcaag cgttcgctga ccttttcttt cttagcttca
     8401 gcacgtgttt gcttgcgaat ccactcctca ttctgctggc gagcctgatc caaatgacta
     8461 gcaatatcat ccagctgacc ttctatatca tccaactcaa actgtttgcg aatcaaacct
     8521 tgttggaggt tcgttttctg agttttggat tcttccgcct gttgactgag caattctgta
     8581 tcaaccttct caagattgtc aaccttttct tgaagaaggc gctgaatttc ttcttgctca
     8641 atatcaagat tatctagttc cttacctaag cgttcaatat cagtcacttc ataacgtttt
     8701 tgcccttgca gttctgtctt aagtagacga gcttgagcta gctcttcctg caagttttga
     8761 taacgttctt ggatggcgtt tttattagac ttaatctctt caatctcagc ttccagatta
     8821 ttcttttcac tagtgattgt agtaaggcgc tcttggcatt tttccttatc cgcttgccat
     8881 tctccctcag aaagacgatt taattcctct tcttggagtt tccaaagagt ttctagctct
     8941 tcaacttgct gactggtctg ctgataggcg agggacaagc cttgctcctg aatacgagcc
     9001 tgctctccct gagatttgat ggcttctaat ctttctgtca atctagctag ctcatcttgc
     9061 aagttcttca aactcacttc atctgaacgc aaacttgctt cttcttcagc aatttctttt
     9121 tgtaattgct ccagttctgg cttgatgaaa atactgttat tctgacgatt ggctccacct
     9181 gcgtaagaac cacctgtgcg caactctgta ccgtccaaag tcaccatacg aacctgataa
     9241 cgaactttgc gcgctgctgc acgcgcatgc tcaacggtat caaaaatcgc tgtcgtagct
     9301 agcaagttct tgaaaatagc ttccagtctc gtatcgaatg tcaccaactc atctgccatg
     9361 cctagaaatc ccgcacttgc agcgataaca tcttggttct gactagaaat cgtacgcgcc
     9421 ttgatagtcg tcaacggaag gaaggttgca cgaccagctc tatttcgttt gaggaaatca
     9481 atcgccttgg ttgccgactc ttcatcttct acgatgatat gctgactgct ggctccaagt
     9541 gcaatctcta aggcagtttg ataatgcaca tcaaaggtca aatgctcact aacagcacca
     9601 ataatcccac caaggcggtc tttttcttgg agaacactct ttacacctgc ataaaagtta
     9661 ctgtgatttc taaggatgtt ttccaaactt tgagctctag cctgcttgtt tttaagacta
     9721 tccagacggt caaagagttg actttgttga gcttgatagg aagttttttg ctcctcttgc
     9781 tccttggcaa tagcttgata gtcagccaat aatttctgaa cctgcacctt ggcagtttca
     9841 agctcgtctt tttgctgact agccttctct ttagctgtag ctaattgctc tttcagcttt
     9901 tctagttgat ctgcttgttt ttgagaaagc tgacgactat tttccaactc attctcgatg
     9961 cgggtcagtt ggtttgagac atccgcttct tcttgtaaaa gcgccacaaa gcgttcacgc
    10021 aaaagctcaa tcatctgatc aggatcatct gaaaaagcta gcaattcagc ttctaaacga
    10081 ttgagttttt gattattttg gactagattt tcctctaaaa gtactaaaga gctttcttta
    10141 tcagattttt cttggctgag tgaatttctc ttatcctcca aaacagccaa acgagcttgt
    10201 gcttcctgtt gattcagggc tacttgctca gattctaatt tcgatagggc taattttctc
    10261 tctaaatcac taatcagact ggtcaaatcc atcaaactgc cttggtcttt ggccatttca
    10321 gcctgtaaat cttggcgttg ctttttaaga gtttgatttt cttcttctaa tttttcacgc
    10381 ttttggtaat aactcatcaa gagttcttga acctgagcca actcttcttc tgtcgactct
    10441 agttcagcct tattttcctt gatttgagca accagaacat ctaaataaat agccttacgt
    10501 tgtccttcca agtctaaaaa cttacgggca ttctcagctt gcttttcaag aggcttgatt
    10561 tgattatcca actcgtagat aatgtcctct aagcggtcca gattatcctg agtttgctgc
    10621 agtttactct cggtttcttt tctgcgagtc ttgtatttta aaactccagc agcttcttca
    10681 aaaatagctc gtcgttcctc aggcttggaa ttaaaaatct cctcaacctt cccttgggaa
    10741 ataatagaga aggaatctcg tcccaatcca gtatccaaga agaggtcatg aatatcacgc
    10801 agacggactt tcttgccgtc aatcttgtat tcgctatctc cactacgata gatatggcgt
    10861 tccaccctga tttcttgacc tgcatccttg ataaatccgt catgattatc cagagtcaca
    10921 actacagaag cataattgag cggtttgcga ctttcggttc cagcaaagat gacatccggc
    10981 atcttgcccc cacggagact cttgacacta gactccccca aagcccaacg cagactttct
    11041 gtaatattgg actttccaga tccattgggt ccaacaactg ccgtcacacc ttggtcaaaa
    11101 acgaccttgg tcttatcagc aaaagacttg aacccctgaa tttcgatttc ctttaaatac
    11161 atgaatccag ccccttctca acggcatttt tggcagcttc ctgctctgct aatttcttag
    11221 aacgaccttg gccttgaccg atgctcttac cttcaacaag aacttctaca tcaaagacct
    11281 tatcatgagc aggccctgtt tcagaaatca cctgataacg aatagccaca tcaccattga
    11341 cctgaagcaa ctcttggaga tgggttttat agtctgtaat catctcaaac tcgcctgctt
    11401 caaccttagg aatgatgact tgatagataa attccttgac cttggccaca tccttatcca
    11461 aaagaagggc accaagaaag gcttcaaagg catcaccaag aatggtgtca cgattgcgac
    11521 cacctgattt ttcttcccct ttacccaact tgataaactg gttaaactgg caatcacgcg
    11581 caaaaccagc taaactctcc tcacggacaa tcatagcacg gagttttgat aggtcacctt
    11641 caggcttttt aggatatttt ttatatagat attctgaaat caataactgt agaacagcgt
    11701 ctcctaaaaa ttccaagcgt tcattgtgtg aaatttttaa gaggcggtgc tcattggcat
    11761 aactcgtatg agtaaaggca gtttccagta aatttttgtc tgcaaattcg attgcaaaat
    11821 gattctttag tacagtttgt aattctttca taccaacctc tttctaactg ataatagtcc
    11881 tttttattat atcaaaaaaa gccccctgag tcactctaaa acgggactgg aaagcatttg
    11941 ggaattcttt agacagagat tctcagtttt agcggcaaat ttgggtcagg ataaagaaaa
    12001 aagccctatt aaaggctttt taggatgttt acatccaccc tgagggaatc gaacccccat
    12061 ctcaagaacc ggaatcttac gtgatatcca ttacactaag ggtggaaact tgttttatta
    12121 taacagaaat ttgctctaat aacaagtttt ttggtcaaag accccgtctt agtgggaagc
    12181 atccccattc cagatggagt ttttcacgat cacataatca acgtgtttaa ggtcagcaac
    12241 ctgacgtcca cctgcataag aaatagcact ttgaaggtct tgttccatct cagttaaagt
    12301 gtcttgcaga tgacctttag caggaagcaa gatacgtttg ccttccacat ttttgtaagc
    12361 acctttttga tattgtgagg ctgaaccata atattctttg aactgttcac catcgacttc
    12421 aatcgttttc cctggacttt caatgtgtcc tgcaaagagg gaaccaatca tgatcatgct
    12481 agcaccgaag cggatagact tagcaatatc accgtgagta cgaattcctc catcagcgat
    12541 aatcggttta cgcgcagcct tggcacacca gcgtagagca gccaactgcc aaccacctgt
    12601 accaaaacca gtcttaacct tggtgataca aaccttacca ggaccgattc cgaccttagt
    12661 agcatccgca ccagcatttt ccaattcacg cacagcttct ggtgttccca catttccagc
    12721 aatgacaaag gtatctggca attctttctt gatgtgttga atcatagaaa tcacgctatc
    12781 cgcatgacca tgagcaatat caatagtgat atactcagga gcatcagcct tgagctgtct
    12841 aacgaaatca tactcataat ccttaacacc gacagagata gaagcaatga gcccttgatc
    12901 gtgcattcgt ttaataaaag gaatgcgtcc tgcctcatca aaacggtgca taatgtagaa
    12961 gtaaccacct ttagccagtt gctctgctat attttcatcc aaaatcgtct gcatattcgc
    13021 tggcacaaca ggtagtttaa aggtgtgatt tcctaaagtg acacttgtat ccgcttctgc
    13081 acggctttta atgacacatt tatttggaat caattgaata tcttcgtaat caaaaattgg
    13141 aaattcattt aacatatcga tgtctcgttt cttttgtaat gacctaccta tgctcttgca
    13201 tcactacgcc ttttccgacg tttccttaat ttattataaa ccaaagtaca gtttttgtca
    13261 aattatttta tgaattgaaa tatattgttc gggttttact tgtgttaaaa ataaaagaaa
    13321 gagaggaaat tattttctcc ccacttcttc atcaataatc gctttaaaat tttcttctga
    13381 attttccatc attttacaaa atgcactata agatgaagac ttctgaggaa tttggataga
    13441 ccatttcttc aacaaagctc cataacctgc taggtttccc atctcattat caataacctt
    13501 gtccttgctc ggaaaaacaa gtccagcctt ctcagctttt aaaatataag aataggaaat
    13561 cagctggaat aagtcctcac ggttgattcc tttttcagtc aattccagtt ttttatattt
    13621 tgcatctaga acaatctttc gttctctgtc ataaaaatct ggatatactt ttcgtttccc
    13681 aacagaaaat actgaaattc catccgtctt atccttattt ctgggatgta caaaaccttt
    13741 tggcaacaag gtgtaaacat actcttccca aagccaggca acatcaaaga gaataccata
    13801 gattttttga tcttgatacc ctaaaccgtg cttttcttgg tttaggatca tcagacaaag
    13861 ttcttgtaag tttctgtact cgtgaaagta tgcatgacgt ataggtttag attgatttcc
    13921 ccgaataatc ttagcacgat cagctagttt ataagagggc gttacacgca cgatttcaga
    13981 tacgttttca cgactagttg agagattatc tagtacccct tgaccaatgc ttttctgatt
    14041 cttaatgtat tcaatagtgt gacggaccaa ctgcatgagg ggattatcat aggtgaactc
    14101 tctcgttgcg taggcaatat ttcccgtgaa aggaagattt ttcttgagat ggtttcttac
    14161 atcaatcact cccttaacat gactgtcgtt atgagaaaat cgatgatatt ccttataaag
    14221 accttttcga atagcagctt gtagatactt gggaaagaga tacaccaaaa gttgataaag
    14281 cctctcttca cgagacaaag caacatctaa actagtgaga ttgatatgaa gaatcttgtt
    14341 taagagataa tgcaaaaaat ggtcattact ctcatcagaa aatcgtgagg aaatcgttaa
    14401 tctttcctga ccatatccaa gaaaaccaat cacgttccct gtcttgattt tctgattgac
    14461 tgtttcaaaa atcttttggt ccttatccaa atcaggagta tgagtcaaat catttggaaa
    14521 aatgaaaata cgttcatctt gggatagact ttccaaggtt ctgtctaaaa gaagattgct
    14581 tagtttagga tattcttcaa caaatttttc tttaataatc ttgtattgat tatcagtaat
    14641 ccgcatcatt atcaccagta tcttgttgac cagtttgatc attatttgtc agttcaaatg
    14701 cttttttcaa agtttccaaa gtttcaacct catcataaga acctcgcaag tagtcttcta
    14761 ggagaggctt aatataatca gaccagagta attcatagtc aaaatctaca tccttcaact
    14821 taagaaaata acttggtcca atatgataat gactgtttaa ttcctgaaca ttttcgatag
    14881 cagcgttcaa gtttcttaga cgaatttttg cttcttctgc atggatattc agctctttat
    14941 ccaacatgcc aacttgaccc tcggcagtaa cttcaacaaa acgaaaacga cgacgcatag
    15001 caaaatcaaa ggtatccact gaacgatcaa tatcattcat agttccgatg atgtaaacat
    15061 tttcggggat atagaacttt tcatcagttt cgtgtagatt tgcatattgg gtagaaacac
    15121 ttcctttttc accacgatag ccagggtcga tagagaaaaa gagttcgcca aaaatcttag
    15181 aaatctcccc acgattgatc tcatcgatga tgaaaacaaa tttcttgtct gtttcaacct
    15241 cttctggaga aatatagtct ttcaaaccaa atctctttct caatgtttct aggacagttt
    15301 ttccaccact tttgtagtat tcttgcttat tataattttt atctttatac aactcgtaaa
    15361 catatttgct aggtagtgac catcctggaa caccactatc ataatttact gacaaatttt
    15421 gtctactatt aacagataag taagatgttt ttgttatata ttctttttct tcagcaacat
    15481 ttatatattc taagtaagaa tcccaagcct catcaaaatt atcttgtcct ccaatcaatt
    15541 gggtttcttt tgctttctga caaaaatctt taaaaatacc gtcctgtagc ctaaactcaa
    15601 tagctccatc cccatttgat actggtctta aaccttctac aaaatccgta taatcatagg
    15661 atgggtgaaa ttgtacaaat ccgatttggt cttcgttacc atccgttaat tctttggcaa
    15721 tttctttagc aagataagtc tttcctgtgc caggagcacc acgaaggata aggtttggag
    15781 actttaatag agattgagtt aattgatgac taaaattgtt tgatgaatcc attgtactat
    15841 tgtcttcctt attttccata aaaagcttag aatactcaaa aagcatagtt cctaatttcg
    15901 atggctccaa ctcaggataa gttttggaca ccagttcaat tattttatga ttttgcagat
    15961 aaggtccacc ttgatttgtc tgagaaacaa agtggttcca aaattctttc tgactattaa
    16021 tatttacacc aaagaaagga tctccctcag tatagataca aagtaatttt gtcaccatag
    16081 cagaacgacc aataaattca tttgttgatc ttttcatatc aaaaatagga ttttcaaagt
    16141 taaaacgaat accttctttg ataatttcat acaaatctga ttttaatttt gaaaatcgct
    16201 gatccaactc tgaaatctca ttattagctt gatctttata tttgtttgtt tttttattcc
    16261 aataaatacc aaattttgaa gctgagccac cagaaattcc aagaaataga tttttgtatt
    16321 ttcccttctc aagagcgtag cataaagact tattttgctg tccctttcct attacatatt
    16381 catcgattga cattgttaaa atcctatcca aaggccattc ctggagaaac tcttctttta
    16441 atttatcatt aaacttttca ccaacagttg ccttatttaa taact
//
LOCUS       DAVLVZ010000039        16478 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000039 DAVLVZ010000000
VERSION     DAVLVZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.039"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            157..525
                     /locus_tag="VYN77_001341"
     CDS             157..525
                     /locus_tag="VYN77_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558793.1"
                     /translation="MRLSKEARVVLEELKLEYLKKGEDKTSGVIVDEIFKHFSSNFQK
                     VDWKSVQSAPKFNEILADYTSVNPTTLNLSNSTISTMSYIRDHFNYILDMKRTVYRSF
                     IIRLVLKAYKLEKEGTDIYL"
     gene            638..880
                     /locus_tag="VYN77_001342"
     CDS             638..880
                     /locus_tag="VYN77_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558794.1"
                     /translation="MKINEYLWRTSCLILLIIMSFFAGFGAKLEFTSYNPFLSVKFGL
                     VVALTVFVVQLLAILPAMVKRGNKNENVESNNSCEE"
     gene            843..2234
                     /locus_tag="VYN77_001343"
     CDS             843..2234
                     /locus_tag="VYN77_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEV1558795.1"
                     /translation="MRMLNQTIRVKSKYPYLIRPRNIKIVRLFIQIFVFLLLISSWIA
                     IIPFIARLVFSMFFISLLFFVEKFFRSNFIAKLRNKLNIREALFYMLISLNLYDELDN
                     EVVDTAVLYFDVENNKVVVCAPLFGNRYLKTLKNLEEYLCPTLGLSLLSKKEEIDKIV
                     YVLGQKEEIEQYVFNSNTLTREFFKDIPSSIIKLSNTQKFSLKSNTNLGIYGRTGTGK
                     TIALQWYLFNALAKGCGIADNTYLGIVDGKAADLYRIGELLHEELGEQVAVGSSPQML
                     AQLSRKFIENMDARFKIIKQNSSLNADIYELDELGLVPNFLFVDELASIRDSCGSTKQ
                     GRETWNEILQNLGLIARKGRQAGCHLVLSTQDPNAENIPVELRNQISAVLYLGAPGAD
                     RLKMAFSMCELENVPTVSDRKGEALFYADGLNTVEPVLTIVPFVDLKTKKEFLNVVRN
                     ILPTNDGLPPLAK"
     gene            2490..2909
                     /locus_tag="VYN77_001344"
     CDS             2490..2909
                     /locus_tag="VYN77_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558796.1"
                     /translation="MVYNMTDTNIKIGLLDLEMSQFDIPKKVVILSVRVNNKYKEVNG
                     ENVKTDEVSKITSAVLDADKVKVLTEMGISTDDLKAINLEIIGNLDKIAKLAQNDALL
                     NVPVELIRPKVRLAWNMARNNWSGVKIVCEDIKVLGE"
     gene            2914..3933
                     /locus_tag="VYN77_001345"
     CDS             2914..3933
                     /locus_tag="VYN77_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEV1558797.1"
                     /translation="MNTVRIDYFAVTVKNISPEKVLTDILLIPLENFTLNDWGINKYK
                     QHYSCSEIKVYFNADRLSMGVFIELKGQGCRQYEEFLNSNENNWIALISRLYHHNANF
                     TRLDIAHDIFDGSLDVQRIYDYCKKGLCISKAKHFEYHEKSVLDSGERVGETVAIGSR
                     GNQQWCIYNKRMEQLGKQELVEVSSWTRAELRCWQEKANIIAEQLYLKRPLSSIYFEA
                     INGHYRFIRPNAIDSNRWRRKKVEWWVNYLGTENQTVLSITRAKTTLRESEVWTEKQV
                     AKTLAKVYIAKYQAYDVQKAEDYIQNLLKEGLSKLTDNDEKDIEQYIREQNSSQLWGT
                     DGRSD"
     gene            3937..4119
                     /locus_tag="VYN77_001346"
     CDS             3937..4119
                     /locus_tag="VYN77_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEV1558798.1"
                     /translation="MADTISNKDLISMGYRPSTANAIIHQARELLVSRGYTFYNRKRL
                     MVVPKSVVKELLGMEL"
     gene            4121..5248
                     /locus_tag="VYN77_001347"
     CDS             4121..5248
                     /locus_tag="VYN77_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1558799.1"
                     /translation="MAYIEYKQRGKKRLWSFSIRERSKSLLHKSGFKTKREAKIEAEK
                     VLHKLNTGSVLSSSMTLSELYNEWLDLKILPSNRSVVTKKKYLMRKKVIERLFGNKPV
                     SQIKPSEYQKIMNEYGETVSRNFLGRLNSCIRASIQMAIADKVIIEDFTAYVELFSSK
                     SGQKVEEKYLHAESDYQKVLVYLKNKFDYQKSIVPYVIYFLFKTGMRFSELIALTWDE
                     VDELNEQLKTYRRYNTAIHKFTPPKNNTSIRLVPITSDMLSLLKTLKILQLKTNKELN
                     IDNENNLIFQHFGYVYDVPDIATVNKAIKVMLKELQIFPLITTKGARHTYGSYLWHNN
                     IDLGVIAKILGHKDISMLIDVYGHTLEEKISEEFMAVKSLL"
     gene            complement(5331..5403)
                     /locus_tag="VYN77_001348"
     tRNA            complement(5331..5403)
                     /locus_tag="VYN77_001348"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5368..5370),aa:Lys,seq:ctt)
     gene            complement(5668..6939)
                     /locus_tag="VYN77_001349"
     CDS             complement(5668..6939)
                     /locus_tag="VYN77_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1558800.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            7209..7448
                     /locus_tag="VYN77_001350"
     CDS             7209..7448
                     /locus_tag="VYN77_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558801.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            7613..7792
                     /locus_tag="VYN77_001351"
     CDS             7613..7792
                     /locus_tag="VYN77_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558802.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            7935..8108
                     /locus_tag="VYN77_001352"
     CDS             7935..8108
                     /locus_tag="VYN77_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1558803.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            8145..8597
                     /locus_tag="VYN77_001353"
     CDS             8145..8597
                     /locus_tag="VYN77_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1558804.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            8888..9358
                     /locus_tag="VYN77_001354"
     CDS             8888..9358
                     /locus_tag="VYN77_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1558805.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            9414..9956
                     /locus_tag="VYN77_001355"
     CDS             9414..9956
                     /locus_tag="VYN77_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558806.1"
                     /translation="MLEKVKQFFRSRSAKTEPSVDILPRNRFADLDFERVLKSGARRL
                     VNEEGRYAEDGKITELEFPEDFAEFEFLVGFKTEEEEQFQQLLARLNSIDNAIQSYLE
                     SEMQQPIPQYAKDLGYTQKRWEKTFYFHPWILSGEENPPNLRYVADYVNDEFTVYFAK
                     KHGRWQAYWDAECQKVIEES"
     gene            10006..10440
                     /locus_tag="VYN77_001356"
     CDS             10006..10440
                     /locus_tag="VYN77_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1558807.1"
                     /translation="MELLNSRMDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPG
                     GGREGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQF
                     DNIIFGDEGQGYKLMPIEEFLNSKQAVPQLQGRLRDYLEESL"
     gene            10437..10910
                     /locus_tag="VYN77_001357"
     CDS             10437..10910
                     /locus_tag="VYN77_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1558808.1"
                     /translation="MITLVRAGAEDLETIIAIQRASFKAVYEKYHDEYDPYLEDCERI
                     KWKLVERPNSYYYFVKEDEENIGFLRVQTNAELTKAWLGTAAILPQYQGKGYGSKGLS
                     LLEKEFPTITQWDLCTVLQDAGMVAFYEKNGYRQIHIEAEKEGMDMVYMKKLIEK"
     gene            11045..11998
                     /locus_tag="VYN77_001358"
     CDS             11045..11998
                     /locus_tag="VYN77_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1558809.1"
                     /translation="MMETWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNV
                     DRFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTAL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKM
                     SIFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNP
                     GMENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDK
                     WDMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            12000..12743
                     /locus_tag="VYN77_001359"
     CDS             12000..12743
                     /locus_tag="VYN77_001359"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1558810.1"
                     /translation="MQQYFVKGSAISPVTIENKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLTQLDQ
                     FDSIIVAYEESGKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            12754..14550
                     /gene="pepF"
                     /locus_tag="VYN77_001360"
     CDS             12754..14550
                     /gene="pepF"
                     /locus_tag="VYN77_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1558811.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLSDMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            14797..15588
                     /locus_tag="VYN77_001361"
     CDS             14797..15588
                     /locus_tag="VYN77_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV1558812.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLYADANQ
                     QSVFYQGLQLEIRNVFSNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFSKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            15699..16367
                     /locus_tag="VYN77_001362"
     CDS             15699..16367
                     /locus_tag="VYN77_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV1558813.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGPIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         5102 a         2794 c         3727 g         4855 t
ORIGIN      
        1 aatgcgaaac taattaaatt taaaaaaaat caactttctt ccttgacctt tttaaaaaat
       61 tttggtatac ttaatgggta atcaagattg aacaaagaat caagcttttt caacttgcaa
      121 aattatcaga aaggaggctc tataatgagg attcaaatga gattatctaa agaagctcgc
      181 gttgttttag aagaattaaa attagaatat ttaaaaaaag gtgaagacaa aacatcagga
      241 gtaatcgttg atgaaatttt taaacatttt agttctaatt ttcaaaaagt agattggaaa
      301 tctgttcaaa gtgcacctaa gtttaatgaa attttggcag attatacttc agttaatccc
      361 acaacactta atttatctaa tagtacaata agtacaatgt cttacattag agaccatttt
      421 aactacatat tagatatgaa aagaaccgtc tatcgttcat tcataattcg cctagtcttg
      481 aaggcttaca aattagagaa ggaaggcacc gatatttatt tataatagat gtttatttca
      541 tctattataa ttttagcatg atgagtagtc aaaactatat ctaaagggag gttaatctct
      601 ctctttattt ttaaaatttg aaaggaaaaa tacaaaaatg aaaattaatg aatatttatg
      661 gagaacgagc tgtcttatac tgctaattat catgagcttc tttgcaggct tcggagctaa
      721 attagagttt acatcttata acccgttttt atctgttaaa tttggtttgg tcgtagctct
      781 gacagtcttt gtagttcaat tgttagccat actgccagcc atggttaaaa gaggaaataa
      841 aaatgagaat gttgaatcaa acaattcgtg tgaagagtaa atacccttac ttgattcgtc
      901 cccgaaatat taaaattgta agattgttta tccaaatttt tgtcttttta ctcttaatat
      961 cttcgtggat tgctataatc ccatttattg ctcgtctagt cttttccatg ttttttataa
     1021 gtcttctttt tttcgtagag aagttctttc gttctaattt tatagcaaaa ttgagaaaca
     1081 agctcaatat cagagaagcc ttgttttaca tgttaattag tttaaattta tatgatgagc
     1141 tcgataatga agtagtagat acagcagttt tatattttga cgttgagaat aataaagttg
     1201 ttgtctgtgc cccactattt gggaaccgtt atctgaaaac cttgaaaaat ttagaggaat
     1261 atctctgtcc cactttgggc ttgtccttgc tgtctaaaaa agaagaaatc gataaaatag
     1321 tatacgtgtt agggcagaaa gaagaaatag aacaatatgt ttttaatagt aacacattaa
     1381 caagagaatt ttttaaagat attcctagtt caataatcaa gctgtcaaat acacagaaat
     1441 ttagtctaaa gtccaacact aacctaggta tatatggaag aacggggaca ggcaaaacaa
     1501 tagctctgca atggtactta tttaacgcac tggctaaagg ttgtggtatt gctgataata
     1561 cttatttagg catagtagat ggtaaggcag ctgacttgta tcgtattggt gaacttcttc
     1621 acgaagaact gggagaacaa gtggcagtag gctcttcacc acaaatgctt gcccaattat
     1681 cgagaaaatt cattgagaat atggatgcaa gatttaaaat tatcaaacag aatagttcat
     1741 tgaatgctga catttatgag ctagatgagc tgggactggt acctaatttt ttgttcgttg
     1801 atgaactcgc ttctattcgt gatagttgtg gctctacgaa acaaggtaga gaaacatgga
     1861 atgaaattct acaaaattta ggcttgattg cacgcaaagg acggcaggca ggttgccacc
     1921 tagtcctatc tactcaagac cctaatgccg aaaacatccc cgttgaattg aggaatcaaa
     1981 tttcagcagt tttatacctt ggtgccccag gggctgatag attgaagatg gcttttagca
     2041 tgtgcgaatt agaaaacgtt cctacagttt cagatagaaa aggggaggct ctcttttatg
     2101 ctgatgggct taatacagta gagccagtgc taacaattgt gccatttgtt gatttaaaaa
     2161 ctaagaaaga gtttttaaat gtagttagaa acatactgcc aactaatgac ggtcttcctc
     2221 ccctagcgaa atagggcgat tgccggaggc agaatcgagc ggagcgctct cttttcgcta
     2281 ggggagggag agccaaccga ctgctttgca ggaggtcaga aaaaacatcc ccagtactaa
     2341 tagggggtgc acacatacat gaaatgtact ctaaaccctt gatacgtgag gggttacggc
     2401 ttactagttg caccaagggg tcgcaaactc ttgggagagt agggatgaga ggtgtttcca
     2461 tgtatgatac ggaattaaag gagaaaaata tggtttacaa tatgacagat acaaacatta
     2521 aaattggatt gcttgacctt gagatgtcac agtttgatat tcctaagaaa gtagtgattt
     2581 tatcagttag ggtgaacaat aaatacaaag aagtcaacgg cgaaaacgtg aagacagatg
     2641 aagtaagtaa aattacttct gctgttcttg acgctgacaa agtgaaagtt ctaactgaaa
     2701 tgggaatttc tactgatgac ttaaaagcta ttaacctaga aattatcgga aatctagata
     2761 agattgcaaa acttgctcaa aatgatgctt tgttgaatgt tcctgttgaa ttgattcggc
     2821 ctaaagttcg actagcttgg aacatggcta ggaataattg gtctggtgta aaaattgtgt
     2881 gtgaggatat taaagttcta ggagaataat cctatgaata cagttagaat agattatttt
     2941 gctgtgacag ttaagaatat ttcgccagaa aaagttctga cagacatcct tttgattcct
     3001 cttgaaaact ttaccttgaa tgattggggc ataaataagt ataagcaaca ttattcttgt
     3061 tcagaaatta aggtgtattt taatgctgat aggctttcta tgggtgtatt tattgaatta
     3121 aaagggcaag gttgtcgtca atatgaagaa tttttaaaca gtaacgaaaa taattggatt
     3181 gctttgataa gtcgtttata tcatcataat gccaacttta ctagactaga tattgcccac
     3241 gacatatttg acggtagctt agatgttcaa cgaatatatg actattgcaa gaaaggatta
     3301 tgtatctcga aagcaaaaca ctttgaatat catgagaaat cagttctaga ttctggtgag
     3361 cgtgtaggag aaacagttgc aataggttca cgaggtaatc agcagtggtg catatataat
     3421 aaacgcatgg agcaacttgg taagcaagag cttgtagaag tttcatcttg gactcgcgca
     3481 gagcttagat gttggcagga gaaggctaat ataattgcag agcagttata tttaaaacgt
     3541 cccttatcgt caatatattt tgaggctatc aacggtcatt atcgctttat tcgtccaaat
     3601 gctatagata gtaatcgttg gagaagaaaa aaggttgagt ggtgggtaaa ctatctaggg
     3661 acagaaaatc agacagttct gagtattaca agggctaaga cgacgttacg ggagtctgaa
     3721 gtttggactg agaaacaggt tgcaaaaaca ttagcaaaag tctatattgc aaagtatcag
     3781 gcctatgatg ttcaaaaggc ggaagattat attcaaaact tgctcaaaga gggactttca
     3841 aagctaactg ataatgatga aaaggatata gaacaatata taagagaaca gaatagttcc
     3901 caactttggg gaaccgatgg aaggagtgac tgacatatgg cagatactat atcaaataag
     3961 gatttgattt ccatgggcta taggccatca acagctaatg ctattatcca tcaggcgaga
     4021 gaattacttg tatcacgagg ctatacattt tataatcgca aacgtttgat ggttgttcca
     4081 aaaagtgttg tgaaagagtt gttgggaatg gaattgtgaa atggcttata tcgagtataa
     4141 acagcgtggt aagaaaagac tttggtcctt ctctatacga gaaaggagca agagcctact
     4201 ccataaaagc ggatttaaaa caaaacgaga agctaaaata gaagcggaga aagttcttca
     4261 taagttaaat actgggagtg tcttaagctc tagtatgact ttatcggagc tttataatga
     4321 atggcttgat ttaaaaattt tacctagcaa tagaagtgta gttactaaaa aaaaatatct
     4381 catgagaaag aaggtcatcg aaaggttatt tggaaataag cctgtatcac aaattaagcc
     4441 tagcgagtat caaaaaatta tgaatgagta tggagagact gtatcgagaa atttcttagg
     4501 aagattgaat tcttgtattc gggcaagtat acagatggct attgctgata aggtgataat
     4561 agaggatttt actgcttatg ttgagttgtt ctcttctaag agtggacaaa aggttgagga
     4621 aaagtatttg catgctgagt cagattatca aaaagtttta gtatatttga aaaataagtt
     4681 tgattatcaa aaatctattg taccgtatgt aatttatttc ctttttaaaa ctggcatgcg
     4741 tttctccgaa ttgattgctc taacttggga tgaagttgac gagcttaatg agcaacttaa
     4801 aacttatcgt cgttacaaca ccgctattca taagtttact ccacctaaaa acaatacttc
     4861 aataagatta gttcctatta cttcagatat gctttccttg ttaaaaacat tgaaaatatt
     4921 gcaattaaaa acaaataaag aattaaatat tgataatgaa aataatttga tttttcagca
     4981 ttttggctat gtctacgatg ttccagatat tgcaacagtc aataaagcta taaaagtaat
     5041 gttgaaggaa ttgcaaattt ttccattgat tactaccaaa ggagctagac acacttatgg
     5101 tagttattta tggcataata atattgattt gggagttata gcgaaaattt taggacataa
     5161 agatatctcc atgttgattg acgtttatgg gcataccttg gaggaaaaaa ttagtgagga
     5221 gtttatggct gtgaaaagtt tgttgtaaaa acacggcaaa ttgcacgaca atttttaaaa
     5281 ggaaagcaaa aaaagcccta tattataagg cttttcctgt tgtatttaga tgccccctac
     5341 agggatcgaa cctgtgaccc acggattaag agtccgctgc tctgccagct gagctaagga
     5401 ggcaaagaaa aaagctgtat tggtgccgaa acttcacggt ttgtattgaa cccgcgcaat
     5461 taagcaggtg ggcaactcgc tctaactgaa gctgtttccg tgtgagacgg cctacatgct
     5521 gttagaagac ttttgtttcc ctaataatac aaaaaatagt cggtcaacac ttaagtgtga
     5581 agtcgtacac cacagcgttt ctatatttat atgataccac tttttcaaaa aaaatcaaga
     5641 ggaaagtgca attttttgaa aaggatttca gatttttcgc aaacggtcga tagcttcctt
     5701 tctttgagcc aaagcccgtt catatttacc agtatcttct gcttggaaat agtgatgatt
     5761 acgaattttt tctggcagat agtcttgctt gacccaattt ccaggatagt tgtgtggata
     5821 gagatagtct tgggcattcc ccagttcctt gcttccactg tagtgcccat cacgcaggtg
     5881 tcgcggaata ggcaagtgcc ctgatgtttt gaggtcagca agtgccttat ccatagctac
     5941 ataggctgag ttggattttg gagaaagggc caaatcaatc acgacattgg caatgagaat
     6001 gcgggcttcg gggaaaccaa tcctttgtgc agcatccaga gcagtcacgg tatgaatctg
     6061 ggcctcagga ttggctaagc cgatatcttc ataggcgata acagtcaagc gacgagcgag
     6121 actaggcaga tccccagcct caatcaagcg ggcagtatag tggagactgg catccacatc
     6181 tgagccacga atagactttt gcagggcaga tagaacatca tagtgaccgt ctccatcctt
     6241 gtccatagtg atgtagcttc tctgaagact attttccatg atgtctaagg tgatgtggcg
     6301 aatgccctcg tcattctcag gggtagagag aacagccaag tccagtgagt taaaggcaga
     6361 gcgaaggtct ccgtttgtag aggttgcgat gaaatccagc gcatcctcat ctagttctat
     6421 tggaaaatca aaaccacgtt cagggttact tagagctatc tgcagggcct ctttgacgtc
     6481 ttggttagac agaggttcca actcgaaaat ttgaacacgg ctacgaatgg caggagtgac
     6541 agagaagaaa ggattttcag tcgttgctcc aatcatgatg accagtccac tttccaagag
     6601 aggcaagagg aagtcttgct tggtcttatc tagtcgatga atttcgtcta gcaataggac
     6661 gagaccacca gaaaatttag cttcttccga gatttcttgc agtcgctttt tactatcaac
     6721 tgtcgcattg aaagttcgaa aggcatactt ggtcgttcca gcgatggcag aggcaatact
     6781 ggttttgccg attcccggag ggccatatag aatcatggag gacaggcggt tggcttccac
     6841 catgcggcgg atgatttttc caggtccgac cagatgctcc tgaccgatga cctggtcgat
     6901 ggttttaggg cgcatgcgaa gcgcgagatt gtctggcata gcagtccttt ctaacgtgga
     6961 tttgagtaga atgactcatc tactatatct tactatgtcc attctagcct attagttgtg
     7021 atatttcaat ggttttgtaa taatcaggga gtgagataga atctttttta ctatttaatt
     7081 taatgaggag aggtatcagc atgagagatt tgattttcct taaagctcaa aataggatag
     7141 ttgagcctaa agaagtggaa gtgctgactt gagaaataat attgctgaat gtcattgtat
     7201 gaactataat gaaaagagag gtgataagca atggaaatga tggaccaagt caggaaattt
     7261 tgatttttac aaaacaaatt cgtcattgga ttcttagtga tcaagttatt tcagggaaaa
     7321 gaaaactttt cttccgggaa gatactccaa aagagatttt agatttgtac gaaaacatta
     7381 aatctaaact tgattttgct taccaagaag tttattctaa taatgggttg aaaaaatatg
     7441 aaaaatagaa taattgatgt ttttgaagta gtaaatcgca tccttgtaat aactgttgaa
     7501 aatcctaatt tcgaagactt gagagttaat catactcaat gaaaatcaaa gagcaaacta
     7561 ggaaactagc cgcaggctgt acttgagtac ggtaaggcga cgttgacgtg gtttgaattt
     7621 gattttcgaa gagtatcagt ttgtaaaaat tggagataaa aaatatagag tacgcagtgg
     7681 tcctatgatt cattcaactc cgcctcaatc tgtcttagat cgagatactt ttacaattga
     7741 ctatacagat gatgaattgc tcgataaaga agcagttttt actactcatt agaaaaatag
     7801 gtctgaataa attggtttcc cttagaacca catctagggg cttttatgta taatagaata
     7861 ataagtatca ctcctttaca atacgtgtaa cacgtgttat aatggtgttg tcaggaggta
     7921 aaagcgctat acctatgaca caaaaagaga tggttaaact ccttacggcc catggttgga
     7981 taaagactag aggcggtaaa ggctcccata ttaaaatgga gaagcaaggg gaaagaccta
     8041 tcacaatcct tcatggtgag ctgaataagt acactgaaag agggatagga aagcaagctg
     8101 ggttgtaaaa ttcagtccct gctttttgat atggaggtag tgaaatgtta gttacgtatc
     8161 cagctttatt ttattatgac gacacagacg gaacagaagc gacttatttt gtccatttcc
     8221 cagattttga atactcagct acacaaggag aggggatttc tgaggctttg gctatggggt
     8281 cggagtggct agggataact gttgcagatt tgatagagag tgatggagag ttacctcagc
     8341 catcagatat caatagcttg tctttaattg ataatgatcc ttttaaagat gatgaagatt
     8401 tcgtgtcaac ctatgacctt gataaatctt ttatttctat ggtatctgtt gatgtatcag
     8461 aatacctagg aagtcaggaa cccattaaaa agactttgac cataccaaaa tgggcagata
     8521 agttgggacg agaaatggga cttaactttt ctcagacttt gacagacgct attgcagata
     8581 agaaagttca agcctaagat taagcgtggt atcgtggtaa tagtcaataa aaaagcacgt
     8641 ccacttgtgc tagttactcg cctattgatt ttatagattt attagccctt tcgcgggctt
     8701 tttatattga tttttatgag aaatgaagaa atcagacttt taagaaaatc actgatacca
     8761 agggtttaaa tgaggtaata tggtataatt aggacataaa ataattttgt ggtaagatgg
     8821 tagtatctat tttagcatat ttccgagcaa tcggggcgat taaagagtcg catagaaaga
     8881 ggacaaaatg gcaacatacg gatttttaga tgttttagag gaagagttgg acaagaactt
     8941 tccctttgac tttgagatta gctgggacaa acgcaaccat gcggtagaag tgagttttct
     9001 attggaagca caaaatgctg caggtgtgga gatggtggat gaagacggag aggtttcatc
     9061 agatgacatt ctttttgagg aagcggtcct tttctacaat cctgctaagt caaccgtcaa
     9121 tgcagaagac tatttaacgg ttatccctta tctacctaaa aaaggatttt ctcgcgagtt
     9181 tttagcttat tttgcccttt tcctcaaaga tactgcggaa gttgggctag ataccctcat
     9241 ggactttttg gaagatccag aagcagagga atttgttatg gaatggaatc aagaagtctt
     9301 tgaagaagga aaagttggct tggaagaggg agaattttac ccttatccga ggtattagaa
     9361 aagattctaa gtggacattt ttaaataatg aattcaagga gaagggggag attatgctag
     9421 aaaaagtcaa acagtttttc aggagcaggt cagctaaaac tgagccgtca gttgatattc
     9481 ttccccgcaa tcgctttgcg gatctagatt tcgagagagt attgaagtca ggagctcgtc
     9541 gccttgttaa tgaggaagga cgctatgctg aggatgggaa aatcacagaa cttgaatttc
     9601 ctgaagattt tgcggaattt gaatttctag ttggttttaa gacggaggaa gaagagcagt
     9661 ttcagcaact attagctcgt ttaaatagta ttgacaatgc tattcagtcc tatttggaaa
     9721 gtgagatgca acaacccatc cctcagtatg ccaaggatct cggctatact cagaagaggt
     9781 gggagaaaac cttttacttc catccttgga tattaagtgg tgaggaaaac ccgcctaatc
     9841 ttcgctatgt tgcagattat gtaaatgatg agtttacggt ttattttgct aaaaaacatg
     9901 gcagatggca agcatactgg gatgcagagt gccaaaaagt aatcgaagaa agctagctcg
     9961 tattttccta gagagtaatt gttatcatag aaaagagtaa atatcatgga attattgaat
    10021 agcagaatgg atttccaagg ttgcaagatt gccctctttt gtggggataa gctactgaca
    10081 attttgcgtg atgacaagga cgatatccct tgtcccaaca tgtgggagtt gccaggtggt
    10141 ggccgtgaag gggacgaaag cccttttgaa tgcgctcggc gtgaagttta tgaggaactg
    10201 ggaattcatt tagatgaaga ctgtctgctt tggagcaaga tttatcctag cgtaattttt
    10261 aaaggcaaga aatcggtctt tatggttggt cagctaagac aagaacaatt tgacaatatc
    10321 atcttcggag atgaaggaca gggctataaa ttgatgccta ttgaggaatt tctcaattct
    10381 aaacaggcag tgcctcagtt gcagggaaga ttgagagatt atttggagga aagtttatga
    10441 taacacttgt tcgagcggga gcagaggatt tagaaactat tattgcaatt cagagagcca
    10501 gctttaaggc tgtttatgaa aaataccatg atgaatatga cccttatctc gaggactgcg
    10561 aacgaattaa gtggaaatta gtcgagcgtc ccaatagcta ttactacttt gtaaaagaag
    10621 atgaagaaaa catcggattt ttacgagttc agaccaatgc cgagttaacg aaagcttggt
    10681 tgggaacggc agcgattttg cctcagtatc aaggaaaagg gtatggatct aaaggtttaa
    10741 gcttactgga aaaagaattt ccaaccatca cacaatggga tttgtgtacg gtgttacagg
    10801 atgcaggcat ggttgctttt tacgagaaaa acggctatcg tcaaatccac attgaggctg
    10861 aaaaagaagg catggatatg gtctacatga aaaaattgat agagaaatag aaaggatggt
    10921 tcagttaaat ttctaaactg aacccgccct aaacactgtg ccaaaaagat aaacttctct
    10981 tagacacaag cgtcttcaga gaatttccta ttttggtttt gtgttttacg ggcttggtat
    11041 cttaatgatg gaaacatggc aagagttaaa agttacagtg aagcgtgagg gagaggagtt
    11101 ggtttccaat ctcttgattg agctgggagc gcaaggtgtt gcgattgaag acagtatgga
    11161 ctatgtggga aatgttgacc gcttcggcga gattttccca gaggtcgagc agcaagaaga
    11221 aatcgtagtg acagcctact accctgacac ggtggatgtg acagtggttg aggcggactt
    11281 gcaggctcgc atatcagaat tgacggattt tatggatttg ggagagttaa aaataggtac
    11341 gactgccttg gctgaggaag actgggcaga taactggaag aaatactatg agccagctcg
    11401 tatcactcat gatttgacca tcgttccctc ttggacagac tatgaggcga ctgcgggaga
    11461 aaaaattatc aagctggatc ctggcatggc ttttggtacg ggaacccacc caactaccaa
    11521 gatgagcatt tttgccttgg aacaggttct tcgtggtggc gaaacggtgc tagatgtggg
    11581 aactggttca ggggttctct ctattgccag ctcgcttctg ggggctaaag aaattttcgc
    11641 ctatgacctg gatgatgtgg cagttcgtgt ggctcaggaa aatattgagc tcaaccctgg
    11701 aatggaaaat atccatgtag cggcaggcga tttgcttaaa ggcgtggaga ttgaagcaga
    11761 tgtcatcgtg gcaaacatct tggcggatat cctcattcat ctgacagagg atgcttatcg
    11821 cttggttaag gatgaaggtt acctgatcat gagtggcatt atcaaggata agtgggacat
    11881 ggtgcgcgag ttggctgaat cagctggatt tttcctcgaa actcacatgg ttcaagggga
    11941 atggaatgcc tgtgtcttta agaaaaccaa ggatatctct ggtgtgattg gaggctagca
    12001 tgcagcagta ttttgtaaaa ggcagtgcta tctctcctgt cactatcgag aacaaggaaa
    12061 ccagtaagca tatgtttcag gttatgcgct tgaaagaaga ggatgaggtt accttagtct
    12121 ttgatgatgg catcaagcgc ttggcgcgcg tgctggatat ggaaaatcgt cagtttgagt
    12181 tggtccaaga attaaatgac aatgtggaac taccagttca agtgaccatc gcatctggat
    12241 ttcccaaggg agacaagctg gagttcatcg ctcaaaaagt aaccgaactg ggtgctagcc
    12301 aaatctgggc ctttcctgca gactggtcag ttgccaagtg ggatggcaag aaattgggta
    12361 aaaaagttga aaaactagaa aaaattgccc ttggagcagc cgagcaaagc aagcgtaata
    12421 ttgtaccaag tattaaactt ttcgagaaaa aagcagattt tctaactcag ctggaccagt
    12481 ttgactctat catagtagcc tatgaagaat caggtaaaga aggagaagcc gctgcgcttc
    12541 tgcaagcagt cactggtctt gaaacaggag ccaaactgct ctttatcttt ggtccagaag
    12601 gcggtctctc acctgcagaa atcgaaagtt ttgaagctaa gggagccgtt ttggtaggcc
    12661 taggtcctcg tattttgcga gcagaaacag caccgcttta cgccttatca gcccttagtg
    12721 ttttagtaga attagagaaa taagaggaag aaaatggaac aaaaacaccg ttcagaattt
    12781 ccagagaagg aactctggga cttaacagcc ctataccaag accgtgagga tttcttgcgt
    12841 gcaatcgaga aagctcgtga agacatcaac caatttagcc gtgactacaa gggtaatctt
    12901 catacttttg aggatttcga gaaggccttt gcggaattgg aacagatcta cattcagatg
    12961 agccatattg gcaactatgc ttttatgcct cagacgacgg actatagcaa tgacgaattt
    13021 gccaatattg cccaagctgg gatggaattt gaaacagatg ccagcgtagc cttgaccttc
    13081 tttgacgatg ccttggtggt agcagacgag gaagtcttgg accgtttggg tgaattgcct
    13141 cacttgacgg cagctattcg tcaggccaaa atcaaaaaag cccactatct aggggctgat
    13201 gtggagaagg ccttgaccaa tctcggtgaa gttttctata gtccgcagga catttatact
    13261 aagatgcgag ctggggattt tgaaatggct gactttgaag cccatggcaa gacctataaa
    13321 aacagctttg tcacctatga gaatttctac caaaatcacg aggatgctga ggttcgtgag
    13381 aaatccttcc gttccttctc agagggactt cgtaagcacc aaaatacggc tgcagcagcc
    13441 tatctggctc aggtcaagtc tgaaaaactc ttgtctgata tgaagggata cgactctgtc
    13501 tttgactatc ttctagctga acaagaagtg gaccgtgtca tgtttgaccg ccagattgac
    13561 ctcatcatga aggactttgc accagtcgct cagagatacc tcaagcatgt tgccaaggta
    13621 aatggtcttg aaaagatgac ctttgcagac tggaaattgg acttggacag cgccctgaat
    13681 cctgaagtga ctattgacga tgcctatgat ttggtcatga agtcggtaga acctttgggg
    13741 caagaatatt gtcaggaagt tgctcgttac caagaagagc gctgggtgga ctttgctgct
    13801 aacagtggca aggattccgg tggttatgcg gcggacccat atcgcgtaca cccttatgtc
    13861 ctcatgagct ggacaggccg tttgagcgat gtctatacct tgattcatga aatcgggcat
    13921 tctggtcaat tcatcttttc agacaatcat caaagttact tcaatgccca tatgtcgacc
    13981 tactatgttg aagcaccgtc aaccttcaat gaattgctac tcagtgatta cttagagaac
    14041 caatctaatg acccacgtca aaaacgcttt gctctggctc atcgcttgac agacacctac
    14101 ttccataact ttatcaccca cctcttggaa gccgccttcc agcgtaaggt gtatacattg
    14161 attgaagaag gagaaacctt cggagcaagc aaactcaaca gtattatgaa ggaagttttg
    14221 acagattttt ggggcgatgc cattgaaatc gacgacgatg cagctttgac ttggatgcgc
    14281 caagctcact actatatggg cttgtatagt tacacttact ctgctggtct tgtcatctcg
    14341 acagctggtt accttcatct taaaaactca gaaactggag ctgaagactg gctcaatctc
    14401 ctcaaatcag gtggtagcaa gacaccactt gagtcagcca tgattatcgg tgctgatatt
    14461 tcaacagaca aaccactccg tgataccatc caattcttgt ctgacacagt tgaccagatt
    14521 atcgcctaca gtgctgagtt gggggagtaa ggggaataga aaaatttttt agaatgatgt
    14581 ttatgggctt gttaagcgtc attctatttg tcttagttgt agctattttc aaaaatgtcg
    14641 ttgaaggtcg tgatgttaaa tttgaactta ctgtagaact ggtattgata gttatttacg
    14701 tcattttata tcagattgta tttaattgag aattcaatat tgagtcattt aagactgcct
    14761 ttttgaacag ttgctagaaa tgtagaggta ttgaccatgt atcaagattt actaagaaaa
    14821 atagcagaag aaaaaccaaa ttataaccag gaggaaatcc agtggttgct tgatcatttg
    14881 ggagatcctt ctccagaaat tcgcgatgac cttgttttta caagctttgc tagagggatt
    14941 caggaagagc tatttacaca ggagcaattt catttcattg ctgaaggggt ttcatctgat
    15001 gtaggactag ataaagagat tgataaggta ggtctgccaa cacttgaacg ttcttttagg
    15061 gcacttgttt atgcaaatct cttgtatgca gatgccaacc agcaatcggt tttttatcag
    15121 ggattacaat tagaaattcg taatgtcttt tcaaatcaag gcttgcacta tctttcaaaa
    15181 gaaaaggata caacaggttt ctcaagtcag tatggttggg ttcatgcttt tgcacatgga
    15241 gccgatttac tgacagaggt ggtctgtcat ccagactttc ctataaacag aattcatgaa
    15301 gtatttgaca tacttggcca actatttaaa agaatgtcga ttcgctttac agatgatgag
    15361 gattggcgtt tagcaagagt gatctatgaa cctattttac aagggaagtt ggagcaagaa
    15421 caagtagctt cttggataaa aactgttgat tttccgatag aagaaaggga ggatttttcc
    15481 aaattttcca actttagatc ctgtctggtg gaagtctatg tccaacttga ccagagaaat
    15541 agtttacaag atgagttgaa agaagctatc cagtcttttc aatactagag attagctaat
    15601 agttctatga ttttcaaaaa atttccaatc aattttcttg aaaccctttc ctacttttga
    15661 tagactagta tctagttttt gaaaaaagga gaaagaaaat gaaaaaattt gttgctgagt
    15721 taatcggtac gttcatgctt gtgttcgttg ggacaggagc tgttgttttt ggaaatggtt
    15781 ttgatggcct tggtcacctt ggaatcgcct ttgcctttgg tttggcaatc gtggtggcag
    15841 cctactcaat cggaactgtt tcaggtgctc acttgaaccc ggctgtttcg attgctatgt
    15901 ttgtaaacaa acgtttgtca tcttcagaac ttgtaaacta catccttggt caggttgttg
    15961 gagctttcat cgcttctggc gctgtcttct tcctcttggc taactcaggt atgtcaactg
    16021 ctagtcttgg tgaaaatgcc ttggcaaacg gtgtcactgt ctttggtggt ttcttgtttg
    16081 aagtcatcgc aactttcttg tttgtattgg ttatcatgac tgtgacttca gaaagcaagg
    16141 gcaatggccc gattgctggt ttggtaatcg gtttgtcatt gatggcgatg attcttgtcg
    16201 gattgaagat tactggactt tcagtaaacc cagctcgtag cttggcacca gctgtcttgg
    16261 taggcggcgc agcccttcaa caagtttgga ttttcatcct tgcaccaatc gctggtggag
    16321 ttcttgcagc ccttgttgca aaaaatttcc ttggaacaga agaataattg aaactcaaaa
    16381 agccttgctc ctcatcttga ggaacagggc tttttcgtat gatactcttc gaaaatctct
    16441 tcaaaccacg tcagcttcac cttgtcgtag gtatggtt
//
LOCUS       DAVLVZ010000040        16474 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000040 DAVLVZ010000000
VERSION     DAVLVZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.040"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            15..686
                     /locus_tag="VYN77_001363"
     CDS             15..686
                     /locus_tag="VYN77_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1558814.1"
                     /translation="MGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEFLEILGK
                     EFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFIRELAGF
                     FSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGNFFAQNT
                     FVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKLISKKTR
                     GGEQK"
     gene            683..1606
                     /locus_tag="VYN77_001364"
     CDS             683..1606
                     /locus_tag="VYN77_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1558815.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            1621..2265
                     /gene="pcp"
                     /locus_tag="VYN77_001365"
     CDS             1621..2265
                     /gene="pcp"
                     /locus_tag="VYN77_001365"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1558816.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            2433..2663
                     /locus_tag="VYN77_001366"
     CDS             2433..2663
                     /locus_tag="VYN77_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1558817.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            2713..2793
                     /locus_tag="VYN77_001367"
     tRNA            2713..2793
                     /locus_tag="VYN77_001367"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2747..2749,aa:Tyr,seq:gta)
     gene            2803..2874
                     /locus_tag="VYN77_001368"
     tRNA            2803..2874
                     /locus_tag="VYN77_001368"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2835..2837,aa:Gln,seq:ttg)
     gene            3036..4238
                     /gene="rpsA"
                     /locus_tag="VYN77_001369"
     CDS             3036..4238
                     /gene="rpsA"
                     /locus_tag="VYN77_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1558818.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(4324..5650)
                     /locus_tag="VYN77_001370"
                     /pseudo
     CDS             complement(4324..5650)
                     /locus_tag="VYN77_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            5844..6341
                     /locus_tag="VYN77_001371"
     CDS             5844..6341
                     /locus_tag="VYN77_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1558819.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            6341..7996
                     /gene="dnaX"
                     /locus_tag="VYN77_001372"
     CDS             6341..7996
                     /gene="dnaX"
                     /locus_tag="VYN77_001372"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1558820.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGANTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            8024..8218
                     /locus_tag="VYN77_001373"
     CDS             8024..8218
                     /locus_tag="VYN77_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1558821.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            8368..9138
                     /gene="sufC"
                     /locus_tag="VYN77_001374"
     CDS             8368..9138
                     /gene="sufC"
                     /locus_tag="VYN77_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1558822.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            9166..10428
                     /gene="sufD"
                     /locus_tag="VYN77_001375"
     CDS             9166..10428
                     /gene="sufD"
                     /locus_tag="VYN77_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1558823.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            10439..11665
                     /locus_tag="VYN77_001376"
     CDS             10439..11665
                     /locus_tag="VYN77_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1558824.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILIEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATVLDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            11652..12092
                     /locus_tag="VYN77_001377"
     CDS             11652..12092
                     /locus_tag="VYN77_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1558825.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            12146..13558
                     /gene="sufB"
                     /locus_tag="VYN77_001378"
     CDS             12146..13558
                     /gene="sufB"
                     /locus_tag="VYN77_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1558826.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(13757..14998)
                     /locus_tag="VYN77_001379"
     CDS             complement(13757..14998)
                     /locus_tag="VYN77_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1558827.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            15101..16210
                     /locus_tag="VYN77_001380"
     CDS             15101..16210
                     /locus_tag="VYN77_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1558828.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         4924 a         2871 c         3605 g         5074 t
ORIGIN      
        1 agtggattag attaatagga atagcaatca ttgttgtggg ttttatttta aaatttgata
       61 caattgcaac agtagtctta gctggtttgg ttacagcttt agtttcaggt gtttctctcg
      121 ttgaattttt ggagattttg ggaaaagaat ttagcaatca gcgagtgctc acgattttta
      181 tggttacctt gcctcttgtg gggctgtcag aaacctttgg actcaagcaa cgatcaatcg
      241 atttgattcg aaagattaaa ggtctgacag ttggaaactt ctatacagtt tatttcttta
      301 ttcgagagtt agctggtttc ttttcaattc gtctaggagg acaccctcag tttgtcagac
      361 ctttggttca acctatggga gaagcagctg cagagtctca attaggtaga aagttaacag
      421 aggttgaaga tgagacaata aaagcgcgtg cggctgcgaa tgaaaatttt ggaaatttct
      481 ttgctcaaaa tacgtttgta ggtgctgggg gagtcctctt gataggggga acattagatc
      541 agttaggcta tgaaagcaat tatgcaggga ttgcttctac atctattatt gttgctgtta
      601 taacacttat tgtagtgggg atttacaatt atttatttga taaaaaattg atatcaaaaa
      661 agactagggg aggagaacaa aaatgacaga gttggcaaag caactattag agttgaccta
      721 tattgtgatt ggttgtcaat ttctccatac agcctattgt agttataaag ataaaacaaa
      781 cccagttcga cttgggacat ctgcattttg gactctattg tctattacgt ttataggtgg
      841 ttcctatatg ccaaatatga gtattggtat tattgtaatc ctattatcgc tgttaacatt
      901 gtttaagcaa gtccgtatcg gaaccttgcc atccttagat gaaatgaaag ccaatattga
      961 atctaacagg ttgaaaaata aaatttttat tccagttatg ctgatggcaa tacttgcgtt
     1021 ggtcttagcg caaatgattc cagaatttag caagatttcg attagccttg ccgccttgtt
     1081 tgctacaatt tctgttcttg tgattaccaa tagtcaccct aagagtctgt tatcagaaaa
     1141 taatcgaatg actcagcaag tttcaacaag tgggattgtt cctcaattat taggggcttt
     1201 gggggctatt tttactgtag caggtgttgg tgatgttatc tctcatctga ttagcggtat
     1261 tgttccttca gatagtcgct ttataggagt tttggcctat gttcttggaa tggttctatt
     1321 cacaatgatt atgggaaatg cttttgcagc attcaccgtt attacagcag gtgttggagt
     1381 tccctttgta tttgctctgg gagctaatcc aattgtggct ggtgctcttg ccatgacagc
     1441 aggttattgt gggaccttat tgaccccaat ggctgctaat tttaacgctc taccagcagc
     1501 attgatggat atgaaagatc agaatggcgt tataaaggct caagcaggtg ttgctctagt
     1561 aatgattgtt attcacatat tcttaatgta ctttctcgca ttttagtaaa ggaaaataaa
     1621 atgaaaatat tggttacagg ttttaatcct tttggaggtg aaaagattaa tccagctttg
     1681 gaggctgtaa aattattacc atctgagatt aatggggctg aagttcgctg ggtagaaatt
     1741 ccaacggttt tttataagtc gtcagaagtt ttagaggcag aaatattacg atatcaacca
     1801 gatgctgtac tttgtattgg acaagcaggc ggcaggaccg gcttaacacc tgaacgagtg
     1861 gctattaatc aagatgatgc tcgcatacct gataacgaag gcaatcaacc aattgataca
     1921 ccgattcgta ttgatggagc atcggcctat tttagtagtt tacctatcaa agcgatggta
     1981 caagctatca aaaaacaggg acttccggca gttgtatcca atagtgcagg aacctttgtt
     2041 tgcaatcatt tgatgtacca agctctttat ttagtggata aaaaattccc taatatgaga
     2101 gcaggtttca tgcatattcc atatatgatg gaacaagtag taaataagcc gaatactgca
     2161 ggaatgagtc tatgtgatat tgtaagaggc atagaggtag ctattgaagc tattgtagac
     2221 tacaaagata aggatttgca gttagtaggc ggtgagactc actgataacc taaaaaggat
     2281 agtcaattat gcttgtttac taactattaa ctatgctaaa tcgattgagg ttgtttacat
     2341 aaaactctat atcagagaag cctgatatag agttttttct tgctagtttt aggattttct
     2401 tgtaaaatag aaaaagtgaa gagaggtatg aaatgagcaa gaaagataaa aaaatcgaaa
     2461 ttcaagtagc ggatgccaaa gttaatgttg gtaaagacag ttttgaaggt tatacattga
     2521 ctatcggtaa aaaagttatc ggagaaattg ccgaattaga cggacaattt gccattataa
     2581 agaatgggaa tgtcgatagt ttttataaaa aattggaaaa agctgtggaa attttgattg
     2641 aaaattataa tttagcaaaa taagtcttgt ttttttgaaa ttttcatgat ataatagtcc
     2701 atgttgattg taggagagat agcgaagagg ctaaacgcgg cggactgtaa atccgctcct
     2761 tcgggttcgg gggttcgaat ccctctctct ccatttcatt aatggggtat agccaagcgg
     2821 taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc ccagttctta
     2881 ggtaataatc aagataaaaa gcaaaatatc ttagggtatt ttatttttat aattgaaaga
     2941 cgtgaatgat atgaacatgt ccttgcgggt gcttaggaaa aaaattataa gtatgtcaag
     3001 tttaagaaaa acttgattgt tggaggattt tttagatgaa tgaatttgaa gatttgctaa
     3061 atagcgttag tcaagttgag actggtgatg ttgttagtgc tgaagtattg acagttgatg
     3121 cgactcaagc taacgttgca atctctggaa ctggtgttga aggtgtcttg actcttcgcg
     3181 aattgacaaa cgatcgtgat gcagatatca atgactttgt taaagtagga gaagtattgg
     3241 atgttcttgt acttcgtcaa gtagttggta aagatactga tacagttaca taccttgtat
     3301 ctaaaaaacg ccttgaagct cgcaaagcat gggacaaact tgttggtcgc gaagaagaag
     3361 ttgttactgt taaaggaacg cgtgccgtta aaggtggact ttcagtagaa tttgaaggtg
     3421 ttcgtggatt tatcccagct tcaatgttgg atactcgttt cgtacgtaac gctgagcgtt
     3481 ttgtaggtca agaatttgat actaaaatca aagaagttaa cgctaaagaa aaccgcttca
     3541 tcctttcacg tcgtgaagtt gttgaagcag ctactgcagc agctcgcgct gaagtattcg
     3601 gtaaattggc tgttggtgat gttgtaactg gtaaagttgc tcgtatcaca agcttcggcg
     3661 ctttcgtcga ccttggtggt gttgacggat tagttcactt gactgaattg tcacatgaac
     3721 gtaatgtatc accaaaatca gttgtaactg ttggtgaaga aattgaagtg aaaatccttg
     3781 atcttaacga agaagaagga cgtgtatcac tttcacttaa agcaacagta ccaggaccat
     3841 gggatggcgt tgagcaaaaa ttggctaaag gtgatgtagt agaaggaaca gttaaacgtt
     3901 tgactgactt cggtgcattt gttgaagtat tgccaggtat cgatggactt gttcacgtat
     3961 cacaaatttc acacaaacgg attgaaaatc caaaagaagc tcttaaagtt ggtcaagaag
     4021 ttcaagttaa agttcttgaa gttaacgcag atgcagaacg cgtgtcactt tctattaaag
     4081 ctcttgaaga acgtccagcc caagaagaag gacaaaaaga agaaaaacgt gctgctcgtc
     4141 cacgtcgtcc aagacgtcaa gaaaagcgtg atttcgaact tccagaaaca caaacaggat
     4201 tttcaatggc tgatttgttt ggtgatatcg aactttaatc aaattgaaaa ttcacaaaat
     4261 cctttgttta ctaaacaagg gatttttctg gctctttgtc aactgtagtg ggttgaagaa
     4321 aagctaagct cgagaaagga caaattttgt cctttctttt ttgatattca gagcgataaa
     4381 aatccgtttt ttgaagtttt caaagttccg aaaaccaaag gcattgcgct tgataagttt
     4441 gatgagatta ttggtcgctt ccagtttggt gttagaatag tgtagttgaa gggtgttgac
     4501 aagcttttct ttatctttga ggaaggtttt aaagacagtc tgaaaaatag gatgaacctg
     4561 cttaagattg tcctcaataa gtccgaaaaa tttctccggt tccttattct gaaagtgaaa
     4621 cagcaagagt tgatagagct gatagtggtg tttcaagtct tccgaataac tcaaaagctt
     4681 gtttaaaatc tctttattgg ttaagtgcat acgaaaagta ggacgataaa atcgcttatc
     4741 actcagttta cgactatcct gttgtatgag cttccagtag cgcttgatag ccttgtattc
     4801 atgggatttt cgatgaaact gattcatgat ttggacatgc acacgactca tagcacggct
     4861 aagatgttgt acaatatgaa agcgatcaag aacgatttta gcattcggga gtgaaacagt
     4921 ctgggagact gtttcagcct gagcctagaa atttgaaagc gaagttgtct agctaagtca
     4981 tagtaagggc taaacatatc catagtaatg attttgacgc gacatcggac ggctctatca
     5041 tatttaagaa agtgatctcg gatgatagct tgtgttctac cttcaagaac agtgatgata
     5101 tcgagcttgt caaaatcttg cgcaataaag ctcatcttca tctcccgatt gaaacagtca
     5161 ctccccggac tgtttcaacg tcccaggaca taatctcagg aagacgcgaa aaatcatgct
     5221 caaagtgaga atcattgagc tttcgaatga cagttgaagt tgaaatggcc agctgatgag
     5281 caatatcggt catagaaatc ttctcaatca acttttgcgc aattttttgg tcgataatac
     5341 gaggaatttg gtgatttttc ttgacgatag aagtttcagc gaccatcatt tttgaacagt
     5401 gatagcactt gaaacgacgc tttctaagta gaattctagt aggcatacca gttgtctcaa
     5461 ggtaaggaat cttagacggt ttttgaaagt catatttctt catttgactt ccgcactcag
     5521 ggcaagatgg ggcgtcgtag tccagtttgg cgatgatttc tttgtgtgaa tccctattga
     5581 cgacatctat aatttggata tttgtgtctt tgatatcgag tagttttgtg ataaaatgta
     5641 attgtcccat atgaatcttt ctaatgagtt gtttggtcgc ttttcattat aggtcatatg
     5701 ggactttttt tctacacaaa aataggctcc ataatatcta tagtggattt acccactaca
     5761 aatattatag agccaaaaat cctttgttta ctaaacaagg gatttttctt ttgtctctgc
     5821 tccttttttg atataatagt tctatgttaa aatcagaaaa acaatcacgt tatcaaatgt
     5881 taaatgaaga attgtccttc ctattggaag gcgaaaccaa tgttttggct aatctttcca
     5941 acgccagtgc tctcataaaa tcacgttttc ctaataccgt atttgcaggc ttttatttgt
     6001 tcgatggaaa ggaattggtt ttaggcccct tccaaggagg tgtttcctgc atccgtattg
     6061 cactaggcaa gggtgtttgt ggtgaggcag ctcactttca ggaaactgtt attgttggag
     6121 atgtgacgac ctatctcaac tatatttctt gtgatagtct agctaaaagt gaaattgtgg
     6181 tgccgatgat gaagaatggt cagttacttg gagttctgga tctggattct tcagagattg
     6241 aggattacga tgctatggat cgagattatt tggaacaatt tgtcgctatt ttgcttgaaa
     6301 agacagcatg ggactttacg atgtttgagg aaaaatctta atgtatcaag cactttatcg
     6361 aaaatataga agtcaaaact tctcccagtt agttggtcaa gaagttgtgg ctaagactct
     6421 taaacaagcg gtggagcaag agaaaataag tcacgcttat cttttttctg gtcctcgtgg
     6481 aacgggaaaa accagtgttg ctaaaatctt tgccaaggct atgaactgtc ccaatcaagt
     6541 gggtggcgaa ccttgcaata actgctatat ttgtcaagca gtgacggacg gtagtttaga
     6601 agatgtcatt gaaatggatg cagcttctaa taatggggta gatgaaattc gcgaaattcg
     6661 tgataaatct acctatgcgc ctagccttgc tcgttataag gtttatatca tagatgaggt
     6721 tcacatgctg tctacagggg cttttaatgc cctcctaaag acgctggaag aaccaacaca
     6781 gaatgtagtc tttattttgg ccactactga attgcacaag attcctgcta ctattctatc
     6841 ccgtgtgcaa cgttttgagt ttaaatcaat taagacacag gatattaagg aacatattca
     6901 ctatatctta gaaaaagaaa atatcagttc tgaaccagag gctgtggaaa tcattgccag
     6961 acgggcggaa ggtggaatgc gggacgcctt gtctattttg gatcaagccc tgagtttgac
     7021 acagggaaat gagctgacga ctgctatctc tgaagaaatt actggcacca ttagcctatc
     7081 agccttggat gattatgtgg cggccttgtc tcaacaggat gttcccaaag ctttgtcttg
     7141 cttgaatctt ctttttgaca atggtaagag catgactcgt tttgtgaccg atcttttgca
     7201 ctatttaaga gacttgttaa ttgttcaaac agggggagca aatactcatc atagttcagt
     7261 ctttgtagaa aatttggcac ttcctcaaaa aaatctgttt gaaatgattc gcttagcaac
     7321 agtgagttta gcagatatta agtctagttt gcaacccaag atttatgctg aaatgatgac
     7381 cgtccgtttg gcggaaatca agtccgaacc agctctatca ggagcggttg aaaatgaaat
     7441 tgctacgctg agacaggaag ttgcccgtct caaacaagag ctttctaatg taggtgcggt
     7501 tcctaaacaa gttgcaccag ctcctagtcg accagctacg ggcaaaacag tctatcgtgt
     7561 cgatcgcaat aaagtgcaat ctatcttaca agaggccgtc gaaaatcctg atttagcacg
     7621 tcaaaattta attcgtttgc agaatgcctg gggagaggta attgaaagtc taggtgggcc
     7681 ggacaaggct ctgctagttg gttctcaacc ggttgctgcc aatgaacacc atgctattct
     7741 tgcttttgag tctaacttca atgctggtca aactatgaaa cgagacaatc tcaataccat
     7801 gtttggtaat atcctcagtc aggcggcagg tttttcacct gagattttag ctatttccat
     7861 ggaggaatgg aaagaagttc gcgcagcctt ttcagccaaa gccaaatctt ctcaaactga
     7921 aaaagaagta gaagaaagcc tgattccaga aggatttgaa tttttggctg ataaagtgaa
     7981 ggtagaggaa gactaaagaa agatttcatg atacaataag tttatgaata aacaacaatt
     8041 tattattatg gcgctattta cagctgctga gacctatttt ttcaatgaag cctggatgac
     8101 tggccgctat attatggcag ccttttgggc aattttactc tttagaaatt tccgagtcag
     8161 ttatgtgatg ggtaaaatcg ttgatgtcat cgatcagcat tttaatagga aagactagcc
     8221 ctcagcttcc agacaaaatc aaagcctttt aggctttttt ttgttatact agaaaagtat
     8281 atttatagaa tttttgctct atttctgggg aaatcagacg tttttctagt aagtactgta
     8341 aaagttttga aaaagaaagg aactatcatg tcagtaatag agatcaaaga tcttcacgtt
     8401 gagattgaag gaaaagaaat tttaaaaggg gttaacctga ccctgaaaac aggagaaatt
     8461 gccgctatca tgggaccaaa tggtacaggt aaatcgactc tttctgccgc tatcatggga
     8521 aatccaaact atgaagtaac taaaggtgaa gttttgtttg atggcgtaaa catccttgag
     8581 ttggaagtgg atgagcgtgc gcgtatggga cttttccttg ctatgcaata cccatcagaa
     8641 atccctggaa ttaccaatgc tgagtttctt cgtgccgcta tgaatgcggg taaagaagat
     8701 gatgagaaga tttcagttcg tgagtttatt actaagctag atgaaaaaat ggaattgctc
     8761 aacatgaaag aagaaatggc agagcgttac ctcaacgaag gcttctctgg tggtgagaaa
     8821 aaacgcaatg aaattcttca acttttgatg ttggagccaa catttgctct tttggacgag
     8881 attgactcag gtcttgatat tgacgctctt aaagttgtgt ctaaaggtgt caatgccatg
     8941 cgtggtgaag gttttggtgc tatgatcatc actcactacc aacgtctttt gaactatatc
     9001 acacctgatg tggtacacgt gatgatggaa ggtcgtgttg tcctttctgg tggtccagaa
     9061 ttggctgcgc gtttggaacg tgaaggatac gcaaaattag ctgaagaact tggctacgac
     9121 tacaaggaag aattgtaatt ccctcgtatc ttttaggaga agtaaatgac tagagaaaat
     9181 attaaacttt tttcagaaat gcacgctgaa ccaagctggt tggctgatct ccgtcaaaaa
     9241 gcttttgaca agattgagac tttggaatta ccagttattg agcgtgtcaa attccaccgt
     9301 tggaatctgg gtgatggaac gattacagaa aatgagccat cagcaaatgt tccagatttc
     9361 acagctttag atcatcactt gaagttggtg caagtaggaa ctcaaactgt tttcgaacaa
     9421 actccagttg agttagctga acagggtgtt gtcttcacag actttcactc agctttagaa
     9481 gaaattccag agctgatcga agaattcttc atgtcatctg ttaagtatga tgatgacaag
     9541 ttggcggctt accacacagc ttactttaac agtggtgctg tactctatat tccagataac
     9601 gtagaaatca cagagccaat tgaaggaatt ttctaccaag atagcgatag caatgtgccg
     9661 tttaacaagc atattatgat tatcgctggt aaaaattcta agattaatta tctggagcgt
     9721 ttagagtcac gcggtgaagg aagtgccaaa gcaactgcta atatcacagt agaagtgatt
     9781 gcacgttcag gtgcgcaagt caagtttgct gctatcgacc gactaggtga aaacgtcact
     9841 gcctacatta gccgtcgtgg taaattaggc aacgatgcaa gtattgactg ggctatcggt
     9901 gtcatgaacg aaggaaatgt cgttgctgat tttgatagtg acttgattgg taatggtagc
     9961 catgctgacc tgaaggttgt agctctttca agtggtcgtc aggtacaagg gattgatact
    10021 cgtgtaacta actatggctg caactctatc ggaaatatcc tacaacatgg ggttatcctt
    10081 gaaaaagcaa ctttgacttt caatggtatc ggccacatca tcaagggtgc taagggagca
    10141 gatgcccaac aagagagccg tgttctcatg ctttcagacc aagcgcgttc agatgctaac
    10201 ccaattcttt tgattgatga aaatgatgtc actgcaggac acgcagcgtc tatcggacaa
    10261 gttgatccag aagacatgta ttacctcatg agtcgtggat tggataaggc aactgcagag
    10321 cgtttggttg ttcgtggttt ccttggatcc gttatcgtgg agattccagt taaggaagtt
    10381 cgtgatgaaa tgattgcaac tatcgaagag aaattgtcaa aacgctaagg ggaagcctat
    10441 gttagatgta gaagctattc gcaaggattt tccaatttta gatcagattg tcaatgatga
    10501 acctctggtc tatctggaca atgctgcgac gacacaaaaa ccactagtag ttctgaaagc
    10561 tattaacagc tactatgagc aggacaatgc caatgttcac cgtggtgtcc ataccttagc
    10621 ggaacgagcg acagcttctt atgaagctgc tcgtgaaacc attcgtaagt ttattaatgc
    10681 aggctctaca aaggaagttc tctttaccag aggaacgaca accagcctta actgggtagc
    10741 acgctttgct gaggaaattc tcattgaggg agaccaggtc ttgatttcag taatggaaca
    10801 ccattctaat atcattccat ggcaggaagc ttgtcgaaag actggagcag agcttgtcta
    10861 tgtctatctt aaagacggtg ccttggatat ggaggatttg cgagctaaat tgactgataa
    10921 ggttaaattt gtttccctag ctcatgcctc caatgtactt ggtgtggtca atccgatcaa
    10981 ggaaatcact caattagccc accaagttgg ggcaatcatg gtagtggatg gtgctcaatc
    11041 tacacctcat atgaagattg atgtccagga cttggatctg gactttttcg ccttttcggg
    11101 tcacaagatg gctggtccga ctggtatcgg tgtcctttac ggcaaagaaa agtatcttga
    11161 gcaaatgtct ccagtagaat ttggcggcga gatgattgat tttgtctacg agcaatctgc
    11221 tagttggaag gaattgcctt ggaaatttga ggctggaacg ccaaatatgg caggagctat
    11281 tggacttgcg actgcagttg attatctgga aaagattggt atggatgccg ttgaagctca
    11341 tgaacaggaa ttgattgcgt acgtctatcc aaaactgcag gcaattgagg gattgaccat
    11401 ttacggttct caggatttgg ctcaacgttc gggtgttatt gcctttaacc taggtgatct
    11461 ccatcctcac gatcttgcga cggttctgga ttatgaagga gtggctgttc gtgctggtca
    11521 ccattgtgcg caacccttgc ttcagtattt ggaagtccca gcaacagctc gtgcaagttt
    11581 ttatatctac aataccaagg cagattgcga caaactagtc gatgccctac aaaagacaaa
    11641 ggagtttttc aatggcactt tctaaactag atagccttta tatggcagtg gtagcagacc
    11701 attcgaaaaa tccacatcac caagggaagt tagaagatgc tgagcaaatc agtctcaaca
    11761 atccgacttg tggggatgtc atcaacctct ctgtcaagtt tgatgcagag gaccgtttgg
    11821 aagatattgc ttttctaaat tcaggatgca cgatttcaac tgcttctgct agtatgatga
    11881 cagatgccgt tttaggaaaa accaaacaag aaattttaga actggcgact attttttctg
    11941 aaatggttca agggcaaaaa gatgagcgtc aaggccaact tggagacgcg gcattcttgt
    12001 caggtgttgc caaattccct caaagaatca agtgtgcaac cctagcttgg aatgccctta
    12061 agaaaacaat tgaaaatcaa gaaaaacagt aagacaagtt tcttttgtct tatgaattat
    12121 tagaaatgaa gaaagaaagg atactatggc tgaagaaaga gtagaaccaa aaccaattga
    12181 ccttggtgaa tataaatttg gtttccatga cgatgtagag cctgtcttat cgacaggaaa
    12241 aggactcaac gaaggtgtta ttcgtgaatt atctgctgct aagggtgagc ctgagtggat
    12301 gttggagttc cgtttgaagt cttatgaaac cttcaaaaaa atgcccatgc aaacttgggg
    12361 agcagacttg tcagagattg actttgatga cttaatctac taccaaaaac catctgacaa
    12421 accagcccgt tcttgggatg atgtacctga aaagattaaa gaaacctttg aacgtatcgg
    12481 gattccagaa gctgaacgtg cttatttagc aggggcttct gcccagtacg agtcagaagt
    12541 ggtttaccac aacatgaagg aagagttcca aaaattaggt attatcttta cagatacaga
    12601 ttccgcactc aaggaatacc cagacttatt taaacaatac tttgcgaagt tggtaccgcc
    12661 gacagataac aagttggcag ccctcaactc agcagtatgg tcgggtggaa cttttatcta
    12721 cgtgccaaaa ggtgtcaagg tagatattcc acttcaaact tatttccgta tcaataacga
    12781 aaatataggt cagttcgaac gtaccttgat tatcgttgat gagggagcaa gcgtccacta
    12841 cgtagaagga tgtacagcac caacatattc aagcaatagc ttgcacgctg ccattgtaga
    12901 aatttttgct ttggacggag cttatatgcg ttatacaact atccaaaact ggtctgataa
    12961 cgtctataac ttggtaacaa agcgtgctaa ggctcaaaag gatgccactg ttgagtggat
    13021 tgatggaaac ttgggtgcca aaacgactat gaaatatcca tctgtttacc ttgatggaga
    13081 aggagcgcgt ggtaccatgc tctctatcgc ctttgctaat gcagggcaac accaagacac
    13141 gggtgctaag atgattcaca atgctccaca taccagctcg tctattgtgt ctaaatccat
    13201 cgctaaaggt ggaggaaagg ttgactaccg tggacaagtc acctttaaca agaactctaa
    13261 gaaatctgtt tcccacattg aatgtgatac cattatcatg gatgacttgt cagcatcaga
    13321 tactattcca tttaatgaaa ttcacaactc gcaagtggct ttggaacacg aagccaaagt
    13381 atctaagatt tcagaagagc aattgtatta tctcatgagc cgtggattgt cagaatctga
    13441 ggcaactgaa atgattgtca tgggatttgt agaacccttt acaaaagaac ttccaatgga
    13501 atacgcagtt gagctgaacc gcttgattag ctatgaaatg gagggatcag ttggataaaa
    13561 tttgatttta tactcttcga aaatctcttc aaaccacgtc agcatcgcct taccgtatgt
    13621 atggttactg acttcgtcag tttcatctac aacctcaaaa cagtgttttg agcaacctgc
    13681 ggctagcttc ctagtttgtt ctttgatttt gaagatgaac ttgtatcaaa aaatcgcggt
    13741 ttaaaatcgc gattttttat aatttctcgt taacaaagcg gacaaactga ttccaccaaa
    13801 cttttaagaa gaaggctttt tcaattttct tgtctgctac catttcgaaa ctagggcgct
    13861 ctgtggtgat gtaaccttga ccaatcaagt ccttgtcttc ataagtcaaa tggccaacca
    13921 ctgttccagc ttcaagtggt gctgggattg ctttggaatc aggtgtgaat tgaacagatt
    13981 gggaagattg attcccaaca cgttcgatta gatagatatc ctctggagcc actgcagtta
    14041 ctgtatcttc ttttccatct tgtacagggg ctttgctatc ttgataggca tcgccttgtt
    14101 gaacgatttt gcgaagtgta aatgtagaag aaatataatc cattagggaa gatgtagctg
    14161 taaatcgagc gtaaggatta ttgtcttgat gatctgcatt taaaacaact gtgatgactc
    14221 tcataccttt ttcgacagta gtaccaacaa aagactctcc agccttatct gttgttcctg
    14281 tttttagccc atcaaaacca ccacggtaag caggcatacc ttctaacatg tagttggttg
    14341 aagtgattgt catcccagca aaagtagaag aaggtttttt ggtgatttct aagacttgtg
    14401 ggtatttttt gatgaggttg cgagcaacga tagcgacatc ataagcacta agcttatttt
    14461 cctcatcttt tttagaacct gggtaaatgt tatcccctag agtttcattg ttaagacctg
    14521 tcgtattgac aacagtggca tcctgaattc cccattccaa gagttttgcc cgcatcatat
    14581 cgacgaaatc tttttctgag ccagcaattt tctcagctag ggcaatagcg gcgctgttgg
    14641 cactagatac cagagttgct tcaagcaact cttcgacagt ataattacgg gcctccatag
    14701 gaatattact ggcttcagaa tttgtcgtca attgataagg ataatcagaa atatctacag
    14761 gagtggagag ggtaatactt ccgttttcca aagcttcata gaccagataa acagtaatca
    14821 attttgttat ggaagcaatt tcgacaggtt gcgttgcatc cttctcatag agaattttac
    14881 cagtatttgc ctcaacagca atcgcatgtt tagcggcaat ggtaaaatct tgagcaacag
    14941 cagtagaagc accccctaaa agagagacag ttaacaaagt taaaaatatt tttttcatag
    15001 tagttttatt ctatcataaa gaaaaaaaat attcttgctt taataattca tctgttaagc
    15061 tttttgaaaa tatggtaaaa taaagtgagg gaggtaactc atgtttcgta gaaataaatt
    15121 atttttttgg accacagaaa ttttactctt aaccatcatc ttttacctat ggagacagat
    15181 ggggtctttg attaaccctt ttgttagcgt gcttaataca attatgattc catttttatt
    15241 agggggcttt ctttattatt tgacaaaccc tattgttact ttcttaaata aagtctgtaa
    15301 actcaatcgt ttgcttggta ttttaattac cttgtgtact ttggtctggg gaatggtcat
    15361 aggtgttgtc tatctcttac ctattttgat taatcagtta tctagtttga ttatatctag
    15421 tcaaactatt tatagtcgag tacaagactt aatcatagac ttatctaatt atcctgcgct
    15481 ccagaatttg gatgtagaag ctacaattca gcagttaaac ttatcctatg ttgatattct
    15541 tcaaaatatc ctaaatagcg tatcaaatag tgtggggagc gtcttgtcag ctcttatcag
    15601 tactgttttg attttgatta tgactccagt ttttttggtt tatttcttat tagatggaca
    15661 taaattcttg cccatgcttg aaagaacgat tctaaagagg gatcgcttgc atattgcagg
    15721 cttattaaag aatttaaatg cgacgattgc tcgctatatt agtggagttt cgattgacgc
    15781 aatcattata ggttgtttgg cttatattgg ctatagtatt attggtttaa aatatgcttt
    15841 agtttttgcc attttttctg gtgtagccaa tttaattcct tatgtggggc caagtattgg
    15901 tttgattcct atgatcatcg caaatatatt cactgatccc catagactgc tgattgcagt
    15961 gatttatatg cttgttgttc agcaggtaga tggcaatatc ttatatcctc gaattgtagg
    16021 aagtgttatg aaggttcatc caatcacgat tttagtttta cttttgttgt caagcaatat
    16081 ctatggtgta gttggaatga ttgtcgcagt gccaacctat tctatcttga aagaaatttc
    16141 taagttctta tcccgtttgt atgaaaatca taaaataatg aaagaacgag aaagagaatt
    16201 agctaagtaa aagtcaggag aaccctgatt tttctttact ggaagtggcc tttagattag
    16261 aagactgaaa ataagttaaa gtcttaaact aattttcaca gctaagaata gtagaagtta
    16321 atctgataaa aatcgaaaaa accagtggaa ttccgtgtca gggtaagttc cactggtttt
    16381 catagtctat taaagttcga atgaaaccta tttatagtat attgaaacta gaatagtaca
    16441 cctctaattc taaaacattg ttagaaatcg attt
//
LOCUS       DAVLVZ010000041        16222 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000041 DAVLVZ010000000
VERSION     DAVLVZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.041"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            32..409
                     /locus_tag="VYN77_001381"
     CDS             32..409
                     /locus_tag="VYN77_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1558829.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            500..832
                     /locus_tag="VYN77_001382"
     CDS             500..832
                     /locus_tag="VYN77_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1558830.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            844..2415
                     /gene="ffh"
                     /locus_tag="VYN77_001383"
     CDS             844..2415
                     /gene="ffh"
                     /locus_tag="VYN77_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1558831.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKVLAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(2610..3893)
                     /locus_tag="VYN77_001384"
     CDS             complement(2610..3893)
                     /locus_tag="VYN77_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1558832.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMLATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(4147..4860)
                     /gene="rsmG"
                     /locus_tag="VYN77_001385"
     CDS             complement(4147..4860)
                     /gene="rsmG"
                     /locus_tag="VYN77_001385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1558833.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            4954..5514
                     /locus_tag="VYN77_001386"
     CDS             4954..5514
                     /locus_tag="VYN77_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1558834.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            5516..6415
                     /gene="htpX"
                     /locus_tag="VYN77_001387"
     CDS             5516..6415
                     /gene="htpX"
                     /locus_tag="VYN77_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1558835.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(6459..8018)
                     /locus_tag="VYN77_001388"
     CDS             complement(6459..8018)
                     /locus_tag="VYN77_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1558836.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLSKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIHSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(8257..8382)
                     /locus_tag="VYN77_001389"
     CDS             complement(8257..8382)
                     /locus_tag="VYN77_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEV1558837.1"
                     /translation="MIELGSLKLNNQERTAWRNYDKFHYEHVKHVLSLYGPVFGF"
     gene            complement(8433..8573)
                     /locus_tag="VYN77_001390"
     CDS             complement(8433..8573)
                     /locus_tag="VYN77_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEV1558838.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKQ
                     KS"
     gene            8743..9285
                     /locus_tag="VYN77_001391"
     CDS             8743..9285
                     /locus_tag="VYN77_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1558839.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            9285..9914
                     /gene="nth"
                     /locus_tag="VYN77_001392"
     CDS             9285..9914
                     /gene="nth"
                     /locus_tag="VYN77_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1558840.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            10125..10646
                     /gene="pyrR"
                     /locus_tag="VYN77_001393"
     CDS             10125..10646
                     /gene="pyrR"
                     /locus_tag="VYN77_001393"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1558841.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            10665..11588
                     /locus_tag="VYN77_001394"
     CDS             10665..11588
                     /locus_tag="VYN77_001394"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1558842.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            11638..12717
                     /gene="carA"
                     /locus_tag="VYN77_001395"
     CDS             11638..12717
                     /gene="carA"
                     /locus_tag="VYN77_001395"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1558843.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            13030..16206
                     /gene="carB"
                     /locus_tag="VYN77_001396"
     CDS             13030..16206
                     /gene="carB"
                     /locus_tag="VYN77_001396"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1558844.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         4779 a         3146 c         3610 g         4687 t
ORIGIN      
        1 gtaggacaag agacaagaaa gagggcctga tgtgaagatt cggatgcgaa atacaattca
       61 gtttgatgag cagttggaag tgattgacca gctttatgat gtggaagtgc atgaaaaagg
      121 agattatagc tacctgcttt tctataatga ggaaaaggaa aaagtagtga ttaaatttca
      181 tggtcaagaa cttgtgatga gtcgattttc caatcccaag accattatgc gctttctaaa
      241 ggatagtgat agtttagcct atattcccac acctatgggt atgcaggagt ttattatcca
      301 aacgaatcgt tatcaagtgg atgggcaaaa gattgaacta gattatcaac tacaaaatca
      361 agagggacat ccctttgcca gctatcaatt ggaaattact tggggctagg ctcatgtctt
      421 tttcaaattt agctatctat cttcttggat cggcttccta gtgtggtaga ttaggctaat
      481 ttttggtata ataaaagtta tggaaatcga aaaaaccaat cgtatgaatg cgctctttga
      541 attttatgcg gcgcttttga cagataagca aatgaattat atcgagctct actacgctga
      601 tgattacagc cttgctgaaa ttgccgagga gttcggtgtc agtcgtcagg ctgtctatga
      661 caatatcaag cgaacagaaa agattctgga agattatgag atgaaattgc acatgtactc
      721 ggactatatt gtccgcagtc agatttttga tcagattttg gagcgctatc ccaaggatga
      781 ctttctgcag gagcagatag aaattttaac aagcattgat aatagagaat aagaggaaga
      841 aaaatggcat ttgaaagttt aacagaacgt ttgcagaacg tctttaaaaa tctacgtaaa
      901 aaaggaaaaa tctctgaatc tgatgtccaa gaggcaacca aagaaattcg cttggccttg
      961 ctcgaggccg acgttgcctt gcctgttgta aaggacttta tcaagaaagt tcgtgagcgt
     1021 gcagtcgggc atgaggtcat tgatacgctt aatcctgcgc aacagattat taaaatcgtt
     1081 gatgaggaac tgacagccgt tttaggttct gatacggcag aaattatcaa gtcacctaag
     1141 attccaacca tcatcatgat ggttggttta caaggggctg gtaaaacaac ctttgctggt
     1201 aaattggcca acaaactcaa gaaagaagaa aatgctcgtc ctttgatgat tgcggcggat
     1261 atttatcgtc cagctgccat tgaccagctt aagaccttgg gacaacagat tgatgtgcct
     1321 gtctttgcac ttggaacaga agtaccagct gttgagattg tacgtcaagg tttggagcaa
     1381 gcccaaacta atcataacga ctatgtcttg attgatactg cgggtcgttt gcagattgat
     1441 gagctcctca tgaatgagct tcgtgatgtg aaagtattgg ctcaaccaaa tgaaatcttg
     1501 cttgtcgttg atgctatgat tggtcaggaa gcagccaatg ttgcgcgtga gtttaatgct
     1561 cagttggaag tgactggggt catccttacc aagattgatg gtgatactcg tggtggtgct
     1621 gctctgtctg ttcgtcacat cactggaaaa ccaatcaagt tcactggtac aggtgaaaaa
     1681 attacagata tcgaaacctt ccacccagac cgtatgtcta gccgtatcct tggcatgggg
     1741 gatatgctca ctttgattga gaaagcttct caggaatacg atgaacaaaa agcccttgaa
     1801 atggctgaga agatgcgcga aaacaccttt gattttaatg atttcatcga tcaattagat
     1861 caggtgcaaa atatggggcc gatggaagac ttgctcaaga tgattccagg tatggccaac
     1921 aatccagccc ttcaaaacat gaaggtggat gaacgccaga ttgctcgtaa acgtgccatt
     1981 gtgtcttcga tgacacctga agagcgtgaa aacccagatt tgttaaatcc aagccgtcgc
     2041 cgtcgtattg ctgctggttc tggaaataca ttcgtcgaag tcaataaatt catcaaggac
     2101 tttaaccagg ctaaacagct catgcagggt gttatgtctg gggatatgaa taaaatgatg
     2161 aagcaaatgg ggattaatcc aaataacctt cctaaaaata tgccaaatat gggaggaatg
     2221 gatatgtctg cccttgaagg aatgatggga caaggcggta tgcctgactt atcagctctc
     2281 ggaggagcag gaatgccaga tatgagccag atgtttggtg gcggtttgaa aggtaaaatt
     2341 ggtgaatttg ccatgaaaca gtccatgaaa cgtatggcta acaaaatgaa gaaagcgaag
     2401 aagaaacgca agtaagacaa gagattgatg caaatctcat ttgatgagaa aataaagtaa
     2461 aaatttctat gataaaacgt acagtatcaa ggttttagaa tatctgatat tatacgtttt
     2521 ttgattttaa taattttgtt aagacacttt tattatggta tacgaaaaat ccactcagct
     2581 gtctgagtgg attaggtgta tttagactct tagtctttat tttcgtatgg caagatcaag
     2641 ttcaagatga ttcctgtcat ggctgaaagg gcagtacctg aaagtgtaac tggaccaagt
     2701 ttaaggatag ctcctccaag tccaagaacc aacatagcac ttgcgatgat gaggtttcgc
     2761 atttgagcga aatcaacacg ttctttaatc aagactttca aaccattgct ggcgataacc
     2821 ccatagagaa ggattgacat accaccaagt acagcgtttg gaatagttga aatcaaggca
     2881 gtgaatttac caaggaagct gagggcaatc gcgatgaagg cagcgttacg gataactgag
     2941 acagaagcga tacgagtcat accgataacc cctgtatttt ctccgtaagt tgtattggct
     3001 ggtccaccaa ggaaggcaga aacagaagtt gcgataccgt caccaagaag agtacggtga
     3061 agacctggtt cttttaagaa ttgacgacca cagatttgac ccaaaacagt atggtctccg
     3121 atatgttcag aaattgttac gatagcgatt ggcaagatag cgatggcttc tggaccaaag
     3181 taaagattgt actctttaaa ggcaccacct gtgctaaatg gcaagtagaa accaggaatt
     3241 tcaaaccagt tagcttctag gacaggcgta aagtcaacca aaccaagagt tagtgcgaag
     3301 aggtaaccac cgataatggc aaagaggaat ggaatgattc gtaggaagcc ttttcctttt
     3361 gtattgataa aggcagcaat taggaaagta acaacggcta ccagagcatt tttccaattt
     3421 ccgtctgcta caagacctgc attggtaaca gctgaacctg caagtccaag accgataacg
     3481 atgatcatag gaccgataat gattggtggc aagagtttat caatccattt tgttcctaca
     3541 aatcggatgc tggtagcaac aaggacatag accaaaccag tcaagataac ccctgtttga
     3601 gcagcagata catccccccc catttctttc atagccagtg acatagctgt gataaaggcg
     3661 aatgaagaac ctagataaac tggaacttta aaaccagtag caagcatgta gatgagtgtt
     3721 ccaacacctg aagcaaaaag ggcaacagat acaggcattc ccaaaatcaa tggtaccaag
     3781 atggtcgcac caaacatggc gaaaacgtgt tggaaactaa ggagaattcc ttttccagcc
     3841 gaaggacgtt ggtcaacgtc tagtaacaaa tcaacagttg attcttgttt catataaacc
     3901 tcacttttgg gcatcaaaaa agagcccatt attttacagc aataggccct cagagtaaga
     3961 cttctttaaa atttagattt taaagagttt taaatatttc tgcgtcttcg tcacctcacg
     4021 ggatgacatt aaaaaccttt gcttaggata gtatagcaga aaaaaatgat tttgtaaatc
     4081 attttttccc gagcctagaa ataaaagagc gaggctgatt ttgtaaacat ttgtttacta
     4141 aaaaatttaa agtgggcgtt tatttggcat accagcctta cgtggatatt tatttggtgt
     4201 ttcttttttc ttttctacca ctgtgatata gcgcggatct ctattcggta gggcgtagct
     4261 gagattgtct tcgaccttac taaaaaggag attgagggca ttcttagctt ctaataattc
     4321 ctcaggcgca ttgctggcct tgagtgccaa tagttttcca ccaaccttaa ggtagggaat
     4381 ggtcaattca gataggacct gcatacgggc aaccgcacga gctgttacaa aatcatattg
     4441 agcacggaag ttcttgtctt gggcaaaatc ttcggcacgt ccgtggtaga aatgaactcc
     4501 gttcaaatcc agttcttgag ccaagagttg taggaagttg atgcgcttat tgagtgaatc
     4561 aataatggtc acatctaact ccggatagag aattttcatt ggtagactag gaaatcctgc
     4621 cccagccccg atatcaagaa gtttgatagt ttcattggga atcaaacctt gaagaatggg
     4681 tgcaatcgaa tcgtaaaaat gtttgagata aacttcttcc ttgtccgtaa tcgccgtcaa
     4741 attaatcttc tcattccact cgaccaagag ctcaaaataa cgttcaaatt gttctttttg
     4801 ctggtccgaa agtggaagat tctgctcggc aagcaagttg taaaatgttt ttggtttcat
     4861 agttatttcc ttctttagca tcatcttatt ttaccacaat catagaaaat ttgatatact
     4921 ggtactaatc taaaagcaga aaggacttat attatgactt ggattattct tggagttatc
     4981 gctcttattg ttatttttgt gattgttagc tataacggtt tggttaaaaa tcgtatgcaa
     5041 accaaggagg cttggagtca gattgatgtt cagttgaaac gtcgaaatga cctcttgccg
     5101 aacttgattg agactgtaaa aggttatgcc aaatatgaag gttctaccct tgaaaaggtg
     5161 gcagaactac gtaaccaagt ggcggcagcg acttcaccag cagaagctat gaaagccagt
     5221 gatgccctca ctcgtcaggt ttcaggtatt tttgcagttg cagaaagcta tccagatttg
     5281 aaagctagtg ctaactttgt taaattgcaa gaggagttga caaacacaga aaataaaatt
     5341 tcttactctc gtcaactcta taacagtgtt gtcagcaact acaatgtaaa attagaaact
     5401 ttcccgagca atattatcgc tggaatgttt ggatttaaag cggcagattt ccttcaaaca
     5461 cctgaagagg aaaagtcggt tcctaaagtt gattttagcg gtttaggtga ctaagatgtt
     5521 gtttgatcaa attgcaagca ataaacgaaa aacctggatt ttgttgctgg tatttttcct
     5581 actcttagct cttgttggtt atgcggttgg ttatctcttt ataagatctg gacttggtgg
     5641 tttggttatc gcactgatta tcggctttat ctacgctttg tctatgattt ttcaatcgac
     5701 agagattgtc atgtccatga atggagcgcg tgaggtggat gagcaaacgg caccagacct
     5761 ctaccatgta gtggaagata tggctctggt cgctcagatt cctatgcccc gtgttttcat
     5821 cattgatgat ccagccttaa atgcctttgc gacaggttct aatcctcaaa atgcggctgt
     5881 tgctgcgact tcaggtctac tagctatcat gaatcgtgaa gaactagaag ctgttatggg
     5941 acatgaagtc agtcatattc gtaattatga tatccgtatt tcgactattg cagttgccct
     6001 tgctagtgct atcaccatgc tttctggtat ggcaggtcgt atgatgtggt ggggtggagc
     6061 aggtcgcaga cgaagtgatg atgaccgaga tggaaatggt cttgaaatca ttatgctagt
     6121 ggtttcccta ctagctattg tactggcacc tctcgctgca accttggttc agctcgctat
     6181 ttctcgtcag agggaatttc tggcagatgc atctagtgtt gagctgactc gcaatcccca
     6241 gggaatgatt aatgccctag ataagttgga caatagcaaa ccgatgagtc gccacgtcga
     6301 tgatgctagc agtgctcttt atatcaatga tcccaagaaa ggtggggggt tccaaaaact
     6361 cttttatacc cacccaccta tctcagaacg gattgaacgt ttaaaacaga tgtaaaagaa
     6421 tccagaggtc gtctctggat ttttgctatg ttgcaaattt ataaatcttc taaattaact
     6481 agcttcaaac cgtgttctgt catgcgatag atgatcgagc agacttcttt taagaaacga
     6541 cggtcatgcg aaacagtgat gagaccgcct ggataggttg caaagagttt tctgatttgg
     6601 ggttgagaag tgggagaaaa gtttcgtgtg ggttcatcca gcaggagaaa gtttggtttg
     6661 cgcaggacta gatccaaaag caggagtttt ccctgttgtc cgccagataa ggagcgaatt
     6721 tgatgctgca tttctggata actgaaattg agactggcta ggtgagagtg gattttctga
     6781 agttcctctt tttccccagt tttgctgata taagctattg gtgataaatc caattgtagt
     6841 tttttgtggt aatcttgtgg cataaaacca agggaaatct ctcttttatc attcagaagt
     6901 tgctttaatt tggctaacag agttgatttc ccaacaccat ttggcccgat aataccgatt
     6961 ttttcttgcc cacggacagt tagttgtagt ttttgaacca aaactcggtc gtcaatggac
     7021 aaattttctt tttccagttg gactaagact ttagaagctg gtaatggttg gatgtctgaa
     7081 aagaaaagtt ggatttgttc ctcttcaagt ggtttttgag tcatggactg agctgccttt
     7141 tcgtagcgtt tttcttgtga gaggacagtt ttcatctttt tagacaatag gcgaccggca
     7201 gtactatctt tggtagctcg aagcgcagtt tctacatttt gcttaactct ccgatgtttt
     7261 tccatggttt tatcgtaggc tctttggttg ttagcagctt gctgactttg tttggcaaaa
     7321 ttagcctttc tctgctcact atagctatca tagtctaaat gctctactag cgtttccgct
     7381 tctttacggt gtttgaccag tcgcaagtga acaatagtgt ctgccgtttc agaaagaaag
     7441 tcttcatcat gggaaatgaa aataacggtt tgcctggtct tttgaatctg gccttttagc
     7501 caatcaactg tctcaaggtc taggtcattt gaaggttcat ctaaaaatag aatctcaaag
     7561 ggtttggcta actcatggat aagctgaatt ttcaaagctt ctccccctga tagattgcca
     7621 atctcttggt cacttgcgaa acgattgcta tcaaaatgca attcctccgc caaacgatag
     7681 aggatactgt agtctaaatc aatagaatct aaaaagaagt agtcgtgtaa agttttcttt
     7741 ttcagctcct cgggaagttt ttgaggaatg taggccagtg actgatagtc agattggatg
     7801 tttcccttga tagtgaaatc agacaaagct tcccccatta aaattttaag taaggttgat
     7861 ttgccatttc cttcttcacc aataatagca accttttccc cgtcttggat ggtcatgctt
     7921 aggtcagata caagatctcg taaatctttg ttttgtgtga tggttagatg attgattttt
     7981 atcatatgat ttccctttct agaatgttca tagtgcataa caaaaagctc tactttacct
     8041 agtagagccc aaagtctatg tcaaaaaaac tctattaaca tgccagaaaa agagcataaa
     8101 agagcacaca aaaagagaca aaaacaagat ctactaaata aaggttcttt atttgataga
     8161 cttagttgtt catgtctccc ttacctccga gtattagtat ctttatatta tacctttaag
     8221 gaaattttgt caacagaaaa ctggaaattc tatgctctaa aatccaaaaa caggtccata
     8281 gagacttagt acgtgtttga catgctcgta atggaattta tcatagtttc gccaagcagt
     8341 gcgttcttga ttgtttagtt ttaggctacc cagttcaatc agaggactgg tgggttagta
     8401 aaatttctca agatcaatta agatactgtt gttcaacttt tctgtttttt gagctcttgc
     8461 atagtagtgt ttagcagctc ttgtagtcga atatcatctg ccgtaccctg tttacagttt
     8521 tggtagcttt tatttaactt ggataggagt tgaatgagtt tcttgtcttc catgtgagca
     8581 tcctccttga tctgatgtac tctttcccta tagtatagca ctaaaaaaca aaactgtcat
     8641 agtaaatatg ttaaaatttc gaattttaat tacaaagttt ggtgtgctga tttgtagcct
     8701 tttcatggta taatcaagag agtaaatctt tatggaggaa gtatgaaatt aaatattcaa
     8761 gaaattcgta agcagtctga aggtttgaac tttgaacaaa cgttagattt agttgatgac
     8821 ctgcgtgcac gtaatcaaga aattttagat gtaaaagata tccttgcagt tgggaaagta
     8881 caatatgaag accgtatgta tttcttagat tatcaactat cttataccat tgttcttgct
     8941 tcgagtcgca gtatggagcc agttgagtta gttgaatctt atccagtcac ggaagttttc
     9001 atggaaggcg caactaacca tctaaatcaa gaaattttag atgatgactt ggtcttgccc
     9061 atcgaaaatg aggcgcttga ccttgctgag agtgtatcag acaatatctt gctaaacatt
     9121 cctatcaagg tcttgacggc tgaagaagaa gctggtcaag gatttgtctc aggaaatgac
     9181 tggcaaatca tgacagagga agaataccaa gctcaaaaag cagtaaagaa agaagaaaac
     9241 agtccttttg ctggcttaca aggactattt gacggagatg aataatggtc ttgtcaaaaa
     9301 aacgagcacg aaaggtgcta gaagaaatca ttgccctctt cccagatgcc aagcctagtc
     9361 ttgattttac caatcatttt gaactcctgg ttgcggtcat gttatcagcc cagacgacag
     9421 atgcagcggt aaataaggcc acaccaggtc tctttgttgc ctttccaaca ccccaagcta
     9481 tgtctgtagc gacagagagt gagattgctt cacatatttc tcgcttggga ttgtatcgga
     9541 ataaagctaa attccttaaa aaatgtgccc aacagttact agacgatttt gatggtcaag
     9601 tccctcagac acgtgaagaa ttggagagtt tggcaggtgt tggtcgcaag acagccaatg
     9661 ttgtcatgag tgtaggattt gggattccag cctttgcagt ggatactcat gtggagcgta
     9721 tttgcaaaca ccacgatatt gtcaaaaaat cagcgacgcc acttgaggtg gaaaagcggg
     9781 tcatggatat cttgccgcct gagcagtggt tagctgccca tcaggccatg atttattttg
     9841 gaagagccat ttgccatcca aaaaatccag agtgtgacca gtacccacaa ttatatgatt
     9901 ttagcaattt gtaagatgct acattgaaac tttttgcttg attttaagca tgctttgtgc
     9961 tatagtaaat gataactaaa tattccttta aacctgtccc gtgaggcagg caaggagcgc
    10021 gataaagtag aagggtgaag tctatactgt gtgcagtagg gctcgcttgg ctataaattt
    10081 caagtctcct tgtttaagga gacttttctt tttggaggtt tgtcatgaaa acaaaagaag
    10141 ttgtagacga attgaccgtc aaacgagcga ttacgcgtat tacttatgag attatcgaac
    10201 gcaacaaaga tttgaataag atcgtcttgg ctggtattaa aactcgtggt gtctttattg
    10261 cccaccgaat ccaagaacgt ttgaagcagt tagaaaatct ttcagttcct gttgtggaat
    10321 tggatactaa acctttccgt gatgatgtta aaagtggaga agatacttct ttggtttctg
    10381 tcgatgtgac agaccgcgaa gttatcttgg tggatgatgt gctctataca ggtcgtacca
    10441 tccgtgctgc tattgataat attgtcggtc atggtcgtcc tgcgcgtgtg agtttagcag
    10501 ttctagtcga tcgtggacat agagaattgc caatccgtcc agattacgtt ggaaaaaata
    10561 tcccaaccag tcgttctgaa gaaatcatcg tagagatgac agaacttgat gaccaagaca
    10621 gagttctgat tactgaagaa gcttagaaag ttaaaggagt agccatgtca gaaaatcaac
    10681 aagcattgaa ccatgtggtg tccatggaag acctcactgt cgatcaagta atgaaattga
    10741 tcaagcgagg aattgagttt aaaaatggag cccagcttcc ctatgaagac catccgattg
    10801 tttccaatct cttctttgag gattctacac ggacacataa gtcctttgaa gtcgcagaga
    10861 ttaaacttgg attggaacga cttgactttg atgtgaagac tagttcggtt aataagggtg
    10921 agacacttta tgacaccatt ttgactctgt ctgctttagg agtggatgtc tgtgtgattc
    10981 gccatccaga ggtcgactac tatagagaat tgattgctag tccaacgatt acgacttcca
    11041 tcatcaatgg tggagatggt tcgggccaac accctagcca gagcttgctt gatttgatga
    11101 ccatttatga ggaatttggc cactttgagg gtcttaaagt tgctattgca ggtgacttgg
    11161 accactcacg cgttgccaaa tccaatatgc agattttgaa acgcttggga tctgaactct
    11221 ttttcgctgg acctgaggaa tggagaagtc aagagtttgc agactatgga cagtttgtaa
    11281 ccattgatga aatcattgat caggttgatg tcatgatgtt tctccgtgtg caacacgaac
    11341 gccatgatag tggagctgta ttttcaaaag aagactacca tgcccaacat ggcttgactc
    11401 aagaacgtta cgatcgtttg aaagaaacag caatcctcat gcacccagct ccaatcaatc
    11461 gtgatgtaga aatagcagac cacttggttg aagcaccaaa atcacggatt gtccaacaaa
    11521 tgaccaatgg tgtctttgtt cgaatggcaa tcttagaatc cgtactagcg agtagaaacg
    11581 ccaactaaaa cacaagacgt taaaagacgc cagtgagcgt ccacgagagg tgaaaatatg
    11641 acaaaaagaa ttctagtatt agaagatggc acagtttttg aaggcaaggc cttcggagca
    11701 gatattgatg taacaggcga aatcgtcttt aatacaggga tgaccggcta ccaagaatcc
    11761 attacagacc agtcttataa tggacaaatc ttgaccttta cttatccttt ggtaggaaat
    11821 tatggtatta atcgtgatga ttatgaatcc attattccaa cttgtaaggg agtcgttgtt
    11881 tttgaagaag cgcgtagagc tagcaactgg cgcaaccaaa tgaccttgga tgaatttttg
    11941 aaagccaaga aaattccagg gatttcagga attgatacgc gtgctcttac caagattatc
    12001 cgtaagcatg gtactatgcg tgcaaccttg acccatgttg gggacagtat ggaccatgtg
    12061 acggaccagc tccaagcaac agttttaccg acagacaata tcaaacaggt ttctactaaa
    12121 acgtcttatc cagctccagg agttggtttg agcgtggtgc tagtggactt tggtctcaag
    12181 cactcaatcc tacgtgaact ttctaaacgc aactgtaacg tgacagttgt tccttattcg
    12241 acaacggctg aagaaattct ccatctcaat cctgacggag ttatgttgtc aaatggtcca
    12301 ggtaacccag aagacgttcc acaagcactc gacatgattc gcggtgtaca aggaaaaatt
    12361 ccaatctttg gtatttgtat gggacaccaa ctctttgcaa tggcaaacgg ggctaagacc
    12421 tataagatga agtttggcca ccgtggattc aaccatgcgg tacgtgaaat cgcaacagga
    12481 cgagtagact tcaccagcca gaaccatggt tatgcagtca gccgcgagga tttgccagag
    12541 cacttgatta ttacccacga agaaatcaat gacaagtcag tggaaggtgt gcgccacaga
    12601 taccaacctg ctttctctgt tcaataccac ccagatgcag ctcctgggcc acacgacgct
    12661 agctacctat ttgacgagtt tatcgagatg atggaagttt ttaaacaatc aaactaagaa
    12721 cgagtaaaag ctcgtcggag aatttatgta actgaacacg gacggaaagc tcagaatgta
    12781 gagaaaccaa cttggattgt caatccttcc ttgatttcct aaaattcaat cgctttctat
    12841 tcgtcctttg tatcttattt aatgtcgccc tgagaggcga cgaatagaaa ggagaagggt
    12901 gctccgtatt tgctgaactg aatacgggct acggacggtg ctaaaaagat agttattcct
    12961 agaactgaca gttcttcgtc ataactccta ttttagcttt gtccgcttga cacccttaat
    13021 atcttatata tgcctaaacg tactgatatt caaaaaatta tggtgattgg ttctggtccg
    13081 attattattg gtcaggctgc tgagtttgac tacgctggga ctcaggcttg cttgtcgttg
    13141 aaagaggaag gttatgaggt tgttttggtt aactcaaatc ctgcaaccat catgacggac
    13201 aaggaaattg ctgacaaggt ttacattgaa ccgattacac ttgagtttgt gacacgtatt
    13261 cttcgtaagg aaggtccaga tgccttgcta ccaacactcg gtggtcagac aggtctcaac
    13321 atggccatgg aattatctaa aaatggtatc ctagacgagc ttggcgttga acttctgggt
    13381 accaaattat ctgccattga ccaagcggag gaccgtgacc tctttaaaca attgatggaa
    13441 gagcttgaac aacccattcc agaatctgaa attgttaaca cagtagaaga agcagttgcc
    13501 tttgcagcga caattggcta cccagtcatc gttcgtccag cctttaccct tggtggtact
    13561 ggtggtggta tgtgtgccaa cgagaaagaa ttgcgtgaaa tcactgaaaa tggtttgaaa
    13621 ttgtcacctg ttacccaatg tttgattgag cgttcgattg ccggtttcaa ggaaattgaa
    13681 tacgaagtga tgcgtgactc agctgacaat gccttggttg tttgtaacat ggaaaacttt
    13741 gacccagttg ggattcacac aggggattcc attgtatttg cccctgcgca aaccatgtca
    13801 gactatgaaa accaaatgct tcgtgatgcg agcttgagca ttattcgtgc cctcaagatt
    13861 gaaggtggat gtaatgttca gctagctctt gatcctaaca gcttcaagta ttatgttatc
    13921 gaagtaaacc ctcgtgtatc acgttcgtca gcccttgctt ctaaagcgac aggatatccg
    13981 attgctaagt tggctgccaa gattgcagta ggtttgacct tggatgaggt tataaaccca
    14041 gttacaggtt caacctatgc catgtttgaa ccagcccttg actacgtagt tgctaagatt
    14101 ccacgtttcc catttgacaa gtttgaaaag ggtgaacgcc gtctcggtac acaaatgaag
    14161 gcgactggag aagtcatggc aatcggtcgt aacatcgagg aatctcttct caaggcctgc
    14221 cgctcccttg aaattggggt gcaccacaat gaaatacctg aacttgcagc agtttctgat
    14281 gatgccttga ttgaaaaggt tgtcaaagcc caagatgacc gtctcttcta cgtatcagaa
    14341 gccattcgtc gtggctacac acctgaagaa attgcagagc ttacaaaaat tgatatcttc
    14401 tatcttgata aactcttgca tatctttgaa attgagcaag aattgggtgc tcatccacaa
    14461 gatctagaag ttttgaaaac agccaaactt aatggtttct cagaccgtaa gattgctgaa
    14521 ctctggggaa cgactgatga tcaagttcgc caacttcgct tggaaaacaa gattgttcca
    14581 gtttataaga tggtagatac ttgtgcggca gagttcgact ctgaaacgcc atatttctat
    14641 tcaacctatg gttgggaaaa tgaatctatc agatctgata aagaatctgt acttgtccta
    14701 ggttcaggtc caatccgtat cggtcaaggg gttgagtttg actacgcaac cgttcactct
    14761 gttaaggcta tccaggcagc tggttatgaa gctatcatca tgaactcaaa cccagagacc
    14821 gtttcaacag acttctctgt atcagataag ctttactttg agccattgac attcgaagat
    14881 gttatgaacg tcattgactt ggaacaacca aagggagtta tcgttcagtt cggtggtcaa
    14941 acagccatca accttgcgga gccattggca aaagcaggtg tgaccatcct tggtacacag
    15001 gttgctgacc tagatcgtgc cgaagaccgt gacctcttcg agcaagctct caaagaattg
    15061 gatattccac agccaccagg acaaacggct accaatgaag aagaagcagc tcttgcagct
    15121 cgtaagattg gcttcccagt tcttgtccgc ccatcttatg tacttggtgg acgtgccatg
    15181 gaaatcgttg aaaacgaaga agaccttcgt tcttacatgc gaactgctgt taaggctagt
    15241 ccagaccacc cagttcttgt cgactcttat atcgttgggc aagagtgcga agttgatgcc
    15301 atttcagatg gtaaaaatgt actcattcct ggtatcatgg agcatatcga acgtgctggt
    15361 gtccactcag gtgactcaat ggctgtttac ccaccacaaa ccttgtcgca aaaggttcag
    15421 gagaccatcg cagactacac caaacgctta gcaatcggtc ttcactgcct tggaatgatg
    15481 aacatccagt ttgttatcaa ggatgaaaaa gtctacgtta ttgaggtcaa tccacgtgcc
    15541 agccgtactg ttccattcct atctaaggta accaatattc ctatggctca agtagcgacc
    15601 aaactcattc ttggtcaaag tttatcagaa cttggctacc aaaatggact ttaccctgaa
    15661 agcacccgtg ttcatatcaa ggcacctgtc ttctccttta ccaaactagc taaggtagac
    15721 agcttgctcg gtcctgaaat gaagtcaaca ggtgaagtta tgggttctga tgctactttg
    15781 gaaaaagctc tctataaagc ctttgaagct tcttatctac acttgccaac ttttggtaat
    15841 gtcgtcttta ccatcgctga tgatgccaaa gaagaagcct tgaacttggc tcgtcgtttc
    15901 caaaacattg gttatggaat ccttgcgaca gaagggacag cagccttctt tgctagtcat
    15961 ggcttgcaag cccaacctgt tggtaagatt ggtgatgacg ataaggatat cccaagtttt
    16021 gtacgcaaag gaagaattca agctatcatt aacacagtag gaacaaaacg aactgctgac
    16081 gaagatggtg agcaaattcg tcgttcagcc attgaacacg gggtgcccct cttcacagcg
    16141 ctagatacag ctaatgccat gcttaaggta cttgaaagtc gtagctttgt tacagaagcg
    16201 atttaaatag aaaaaataag at
//
LOCUS       DAVLVZ010000042        15534 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000042 DAVLVZ010000000
VERSION     DAVLVZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.042"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(257..1864)
                     /locus_tag="VYN77_001397"
     CDS             complement(257..1864)
                     /locus_tag="VYN77_001397"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1558845.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2254..2841)
                     /locus_tag="VYN77_001398"
     CDS             complement(2254..2841)
                     /locus_tag="VYN77_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1558846.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2969..3418)
                     /locus_tag="VYN77_001399"
     CDS             complement(2969..3418)
                     /locus_tag="VYN77_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEV1558847.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGMSFILELV"
     gene            complement(3423..3812)
                     /locus_tag="VYN77_001400"
     CDS             complement(3423..3812)
                     /locus_tag="VYN77_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV1558848.1"
                     /translation="MKVNIVDLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3840..4490)
                     /locus_tag="VYN77_001401"
     CDS             complement(3840..4490)
                     /locus_tag="VYN77_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1558849.1"
                     /translation="MKDKQTFLMKGSFALLLFVILGYMVKFYPETLVGFDQSIQTAIR
                     GDLPDYLTILFRSLTHLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASFCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4480..5043)
                     /locus_tag="VYN77_001402"
     CDS             complement(4480..5043)
                     /locus_tag="VYN77_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1558850.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5208..5442)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5496..5999)
                     /locus_tag="VYN77_001403"
     CDS             complement(5496..5999)
                     /locus_tag="VYN77_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1558851.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6192..7022)
                     /locus_tag="VYN77_001404"
     CDS             complement(6192..7022)
                     /locus_tag="VYN77_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1558852.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(7007..8689)
                     /locus_tag="VYN77_001405"
     CDS             complement(7007..8689)
                     /locus_tag="VYN77_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558853.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LISDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8791..9048)
                     /locus_tag="VYN77_001406"
     CDS             complement(8791..9048)
                     /locus_tag="VYN77_001406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558854.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9055..9603)
                     /locus_tag="VYN77_001407"
     CDS             complement(9055..9603)
                     /locus_tag="VYN77_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1558855.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9618..10466)
                     /locus_tag="VYN77_001408"
     CDS             complement(9618..10466)
                     /locus_tag="VYN77_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1558856.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVCQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10654..12003
                     /gene="trkA"
                     /locus_tag="VYN77_001409"
     CDS             10654..12003
                     /gene="trkA"
                     /locus_tag="VYN77_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1558857.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            12007..13446
                     /locus_tag="VYN77_001410"
     CDS             12007..13446
                     /locus_tag="VYN77_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1558858.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13572..15251)
                     /locus_tag="VYN77_001411"
     CDS             complement(13572..15251)
                     /locus_tag="VYN77_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1558859.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            complement(15321..>15534)
                     /locus_tag="VYN77_001412"
     CDS             complement(15321..>15534)
                     /locus_tag="VYN77_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1558860.1"
                     /translation="IRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYG
                     LFFVDYETQKRYIKKSAFWVKGLKRN"
BASE COUNT         4692 a         3524 c         2809 g         4509 t
ORIGIN      
        1 cacatcttat ttcaattcac tatacaatcc agttcctaaa actttgctag aaattgattt
       61 ggctttccga attaatttgt tcaaatttta tttcatttca atatactgat aaatccattc
      121 actagcacag ggcaagaggg agtcgctatt gatttcaacg gtagcaggta catacagagg
      181 acccctacct cagatatact gcaatatcat cgtatgcaaa aaacctctga gatttttctc
      241 aaaggttctg attttgctaa ttgctgttct caaccgctgc agtaacaaag gcagtgtaga
      301 gttcttctgg tcggtttgga cggcttgaca gttcagggtg atactgacaa gctacaaaga
      361 atttattttc aggaatttcc acgatttcta ccaaacgatt gtctggagaa actcctgaaa
      421 agacaaaacc tgctgcctca aactgctcac ggaaggcatt attaaactca taacggtgac
      481 ggtgacggcg ttgcaccact tcttgattgt gataagcagc agcagcctta gagccacgtt
      541 tcaacttaga cggataaagt cccaaacgaa gggttccacc catatcctca atatcaatct
      601 gatcacgcat gatatcaatg atagggtatt ttgtttctgg tgcaagctct gcagaattgg
      661 caccttcaag acctaaaacg tgacgagcaa actcgataca tgtcaactgc attcccaagc
      721 agactcccaa cattggaaca tcattttcac gcgcatagcg gatggcttgg attttccctt
      781 ctgtaccacg ttgaccaaaa ccacctggta cgatgatccc gtccgcatca gacaagagtt
      841 ctgctacatt ctctgctgtc acatcattgg cattgaccca attgatttta acttctacat
      901 cattgacata gccagagtgt ttcaaggctt cgaccactga gatataggca tcttgcaact
      961 ccacatactt accaacaagg gaaatcttaa cttgtttctt gaggttcatg accttgtcca
     1021 ccatggctga ccattctgtc atatccgctg ctggtgcgtc taatttcaaa tgatcacaaa
     1081 caatttggtc catcccttgt gcctgcaagt tcagtggaat ttggtaaagg tgttcaacat
     1141 ccaacgattc gataacggct tctggtgcca catcacagaa ctgggccagt ttatttttaa
     1201 ttccttgacc agctggctct tctgtacgaa taaccaacat atttggttgg attcccaagc
     1261 cacgcaattc tttgacagag tgttgggttg gtttggtttt catttcacca gcagccttga
     1321 ggtaaggaag caaggttgta tggatataca tgacattatc cgcacccaca tctgccttca
     1381 tctgacgaag agcctctagg aatggcaagg actcgatatc tcctactgtt ccaccaacct
     1441 ctgtgataat gacatcagag tcggtcgtta gagcggcacg cttgattttt tctttcaaag
     1501 catctgtgat atgaggaatg acttgaacag ttgccccaag gtattctcca cggcgttctt
     1561 tacgaagaac ttcactgtaa attttcccag ttgtcacgtt ggaatatttg ttgagattga
     1621 tatcgatgaa acgttcatag tgacccaagt ccaaatctgt ctcagctccg tcatctgtca
     1681 caaaaacttc cccgtgctgg taaggactca tggttcccgg atcaatattg atataagggt
     1741 caaacttttg aatggttact ttgagaccac gatttttcaa gagacggcct agactcgctg
     1801 ccacaatccc tttcccaatg gacgatacca caccaccagt tacaaaaata tatttcgtag
     1861 acatagattc ctctttctaa aatgctcaag gtcttgttaa ctacgagggg atatagaaaa
     1921 caagacccta ctaataagaa taatctggga cgactgcacc agattcacaa ctaatactct
     1981 tcgaaaatct cttcaaaccg cgtcaacgtc gccttgccgt agatatgtgt tcctgacttt
     2041 gtcagtctta tctacaacct caaaacagtg ttttgagcag cctacggcta gtttcctagc
     2101 ttgctctttg attttcattg agtataaaat acaagaagat aacttcttga aaaaacaaaa
     2161 atagctccct aagaactagg gagccccgac ctctaaaaga ggtgcccaaa caatatgata
     2221 cctaaaaata ggataattgt caatagctaa ctcttattcc tcgctggttt cagcgtcgtc
     2281 atctgaaaac tcgtcgtcgt cttcgttgag atccacatct tctcccaagt catctggagc
     2341 gatttcgttg atttctgcat cataagcttc cacttcattt ttttcatcat ctggattttc
     2401 atcatcgtat gaaagagctg gatctgcttc gtatgcatct tcgtcttctg gatcatctgc
     2461 attgtagtca atggcatctg aatcaccatc cataaaggca ttaacacgtt ttttcttagc
     2521 ttttggtgct acttcatcgt cgtcattttc ttcaagagcg atgatttctt cgtcgatttc
     2581 gtccacacca taccatgaac gaagacccca tttgttgtcc ccaagtgaga tgaagctacc
     2641 gtcaaagttc aactctgtgt agaacaaagg caaagcttcg cggatatcgc tgtttgatgt
     2701 tccaaggtag ttttgaattt cgtttacaag atcgctaaaa tgcatctcat gatcgcgacc
     2761 acgaagttcc aagatagcac gcgctacctc aatcatagat agttcacttt tttcttgccc
     2821 agcaaatact tctaattcca aagcgtttct cctcatttat actactatcg ccagagcgaa
     2881 cagactctga cctcatttta tcatttactc tttattttac gataattttg cggaatagtc
     2941 aaaggttaag ggggagaaag tggcaggatt agactaattc caatataaaa ctcattcctt
     3001 tttctgttgc tccattttcc acaaatccaa gcgacttgaa acacctccta gaagcatgat
     3061 tgtaggtgta gatttccttg actctcaatt ctttccatcc ttttactcga gccaattcaa
     3121 tcaaagcact tagaatcttt tttccaagtc ctcgatgttg gtaagcggaa ttcccaatca
     3181 caatggggag attatcctga gatagtgtaa tatccccaat tggaaaccat tctcccttct
     3241 ccttgacttc aatccaaaaa agctcaccat gtcgatccaa ataggaatac atggcttcca
     3301 aggtcgcttg actgtaagga agcttcaccc catctacgag gtaaaccaag ttcacatcct
     3361 gataccaagc aaaagcttcc tcacaatgat tgaccttata ataaggaaca aaacacaatg
     3421 cactaccttt tcaacaaaag agttgctgct tgattaaaac catcacacca gttataccat
     3481 tttgcttcat acccatcttg agctaggata cgatcttcta aatcaaaaac agagtaaatc
     3541 tttctttcct cgcaagcttg cgcatagaga tgatatagtt catcaccacc atctctatcc
     3601 cactcagcag aaatcgtatc ccgacctgcc aataaagcct gataagccct gtgatgccca
     3661 tctgtaatca gcaaacaatc tccaaaggca agaatactga ttggatcgac ttggtttgtt
     3721 tctgccgact ggtaaagcat ctgaatatct tgcaacttct tttctgataa atatagttga
     3781 gtcggatgaa gatctactat attgactttc atttctttct cctcaaggga attcgatact
     3841 cacttctgtt tgcctttaaa tcgccattgg aagcggagct tgtcatagaa gggaaactcg
     3901 ataaacaaga ctcccaaacc cacacagaaa ctggcaagaa catctgatgg gtagtgaact
     3961 cccagataga ctcttgatac cagcacactg actaggtaga ggccaaggac gatttgtacg
     4021 atttttctcc agacctgatc tttaatccgc tgactaagaa taacaatcaa agtccctacc
     4081 atcagcgtta cagctagaga atgcccactt gggaaggaaa atcccttctc ctccaccaga
     4141 tgtaagatag ctggtcgtgg gcgctggtag atatttttaa aggtcacgat taaaagacct
     4201 gccaaagcca gatttcccag catgaagaaa ctttctatct tccatcgctt acgataaaag
     4261 acaaaagcta taatgacaac ccaagtgata atcactggaa tatcaatcag atgtgtgagg
     4321 gatcgaaaaa gaatagtcaa ataatctggt aagtctcctc gaatggcagt ctgaatcgat
     4381 tggtcaaaac cgaccagcgt ttcagggtaa aatttgacca tatagccaag aataacgaaa
     4441 agtaaaaggg caaaactgcc cttcattaaa aatgtttgtt tatctttcat agtgttttaa
     4501 ggttggtttc aaaaggacgt acaacaacca gaatgaaacg gaaaagatta caccctcaat
     4561 caagttaaaa ggtaatacca tggtcattag gtagttggaa agtcccaaaa tttttccaat
     4621 atcaaagtta gcaaacttag cgtacaaagg aacagcataa acatagttga gaaccaacat
     4681 ggccaaggtt aaaccaacag ttccagctag agagcctagt aggaaacgaa gggttgtccg
     4741 ttcctttttc caaatcaaag caaatacgat gacaaaaact cccaaagcta cgatattcat
     4801 cggcaaacca atgtaagtat tcactccttg gctgttaaga agcaatttca agagtgagcg
     4861 aagcaagagc actcctagag cagcaggcaa atccatgacc accagaccca caaggactgg
     4921 caagatacta aattcgatct tgaggaaaga tgccgctggt aaaagcggaa agtcaaagta
     4981 catcagcaca aatgagatgg ctgatagaat tgcaatggtc gaaagtcgac gtgtgtttgt
     5041 cataacaggt tcctccaatt ttctataaaa tcagaagaag ttggaaagga ttcctctatc
     5101 tattctcact ttttatatcc caaaagttcc ctcttactct attaaagaaa aacaaagcaa
     5161 gtggttacaa tccggctata aatctatcaa aacagacaaa gctattcttt cgtcttctcc
     5221 catccagact atactgtcgg ttgtggaatc tcaccacatc agcttgcgct cgcggacttc
     5281 tttaaagaga aagaaataga gatttttctt ttagactagg cgacaagctg taggcataac
     5341 ttctggttag gcaaagcttg tcaacaaagt ataaaaggaa aagatcattt cctagttaaa
     5401 gtcaccgccg gtcgggaatc acaccctgcc ctgaagactc ttttatcata acaaaaaacg
     5461 cttgcaagcg caagcatttt gttcattttc attttttatt tcaatttatc cacttcatag
     5521 gtatggacga gctcaagacc tgcaaagttc tgctggcgaa gagcctcata gacaatcatg
     5581 cagacggtat tagacacatt gagactgcgg acatgttcat cattcatggg aatacggaga
     5641 gccttctcag gatgttctcg cataaaatct tcaggtaagc ccttgtcttc acgtccaaag
     5701 agaaaataat ggtcctcgtc agtcgataaa tccacctcag aatagatttt ttccgcgaat
     5761 ttcgaaatca gatagagttt tcccttcatc tgagatatga aatcttctaa actctcgtaa
     5821 aaataaatct ctagcttatc ccagtaatcc aatccagccc gcttcatctt gcggtcatca
     5881 ataggaaagc ccataggctt gatgatgtgg aggggagaat tggtcgcagc gcaagtacgc
     5941 gcgatattgc ctgtattttg tggaatttga ggttcaaata atacaatgtg atttgtcatg
     6001 acttgcttcc tttcaccatt gcaaaaaaat agccacactg cccggagtca agctcagcaa
     6061 acagcgtggt taaggcatcg ttaacttacc tcacaacagg tttgaagtaa atcagcgaaa
     6121 ctactttctt agtataacac tttcagaatc attgtcaata gaaatgactt gattttttca
     6181 attttttcaa gctatttcca agggttgtaa aatcgtccct gattctgcaa gataagtagt
     6241 aaactaacta ctaaaaacaa ggttgccaag agcaaggtaa tatagtctcc ttttttcaag
     6301 gcctgataac tataccatgt gcgttttttc tctttcccaa agcggcgaag ctccatggca
     6361 gtcgcaatgg tatcaatgcg ttctagcgag ctaaaaatca agggcgtaat aatgagcaga
     6421 ttgcctttga ttcgttgcat aagagaagct ttcttggata attccatccc acgcgcctcc
     6481 tgagacatct tgatagtaaa gaattcttcc tgcaaatctg gaatatagcg caaggtcagg
     6541 ctgacagaat aagcaatctt atagggcaca ccaatttgat ttaaactgga agcaaactga
     6601 ctaggatggg ttgtcatcaa aaagataata gccagaggaa tggtgcaaag atacttaatg
     6661 gccaaattta gcagataaaa gagctcctgg ctggttagag tgtagacacc gattccctgc
     6721 caaatcacac ttctctctcc ataaagtcca accccatact cgggagaaaa gagatagacc
     6781 atcaaaacgt ttaaaacggc aaataccgtc gcaaaaacgg ctacaaagga aacatcttta
     6841 aagcgaattt ctgataaata gaggagaaag actgaaaaga tggcaatcag caagagcatt
     6901 ctggtatcat agctaatcat ggccgccaat gataccagaa tgaaaaagag aagtttccca
     6961 gctcctgaca agcgatgaat cacagtatct ctatgctggt aaccgattaa tttagcttgc
     7021 atccctctct cctttctttg taaaatgccg ttaaatccag tggatccaca tctagtttct
     7081 tagccaagtt aaagatggag gtttctttta gattggcttt tactaacagc tcaggatcgc
     7141 tcaacagact ggctggaaca gtatcggcaa tcaattctcc atccaccatg acaaggaccc
     7201 ggtctgaata atccagcatc aattgcatat catgggtaat catgacaatg gtatgccctt
     7261 tttgatgtaa ctcttcgaga aattccataa tctcagtata gttcttctga tcttgacctg
     7321 cagtcggttc atctaggaga ataatttcag ctcctaagac caaaattgaa gcaatggtga
     7381 cacgtttttt ctgaccaaat gacagggcag aaataggcca attacggaat tcataaagtc
     7441 cacagatttt caaggtttca tagactctcg tttcaatttc cttctcatcc acacctcgca
     7501 aacggagccc tagagccacc tcatcaaaaa tcatattggt tgaaatcatt tgattaggat
     7561 tttgtagcac atagcctact cgttccgccc gctctgcaac agaatcgcct tttatatcct
     7621 gtttttccca aagatagcgt ccttccgtct gaataaagct acttatagcc ttggctagag
     7681 ttgatttccc tgctccattt tttccgacaa tagcaatctt ttcacccttt ttaatatcta
     7741 aatgtaggga ttttaaaatc ggtctatcat cataagagaa agacacgtcc tctaatttaa
     7801 agagtgactg caattctgga gtttcttttg ccagttcatt ctgcagctga acctgacctt
     7861 ttgagataga catgttatcc agattcgcta actgttcttc cttgactaag tccacaccta
     7921 attgacggag agtcgttaga taaaggggtt ctcgaattcc attttgagtc aataaatcag
     7981 tcgcaagcaa ctggtcaggg ctcccattaa aaaggatacg accatcgctt atcaagacaa
     8041 tccgatccac agggcgatgc agaacatcct ccaaacggtg ctcgataatc agagtcgtcg
     8101 tcccctcttc cttatgaatc tggtcaatca attcgataat atcctgacct gacttgggat
     8161 ctagattggc gagtggctca tcaaacaaga gaatcggact ttcatcaatc aagacaccag
     8221 ccagactgac tcgctgcttt tgtccacctg acaaatcctg aggacgctga tccagtaaag
     8281 gaagaaggtc cagcttttca gcccatttat aaacacgacc tttcatctca tctagggctg
     8341 tcacatcatt ttccagagca aacgccaaat cttctgccac agacaagcca ataaactgcc
     8401 catctgtatc ctgcaaaact gtgctaacca gatgagactt atcatagatg ctcatatcaa
     8461 aggctacttg accctttatc aaaaattctc catatgtctg acccttgtaa atattgggaa
     8521 taatcccatt caaacactga cccaaggtag atttacctga cccagatggt ccaacaatta
     8581 agactttctc tcccttgtaa atggtcaagt ctatcccttg caaggtcggt tcttgttgtg
     8641 tttcataccg gaaagagaaa tccttccact caattatagc ttctttcatc ttactctctt
     8701 cattcgcttc ttagacttct attttatcat aaatcaagcc cttcttgcag tctcttctct
     8761 taaaatctta gcgccaaaaa gattcctatc ctagcttact tgcctaacta atctataaac
     8821 atcgaaaaag actagttgcc cagccttccc catcatttta tactcttcga aaatctcttc
     8881 aaaccacgtc agcttcgcct tgccgtaggt atggttactg acttcgtcag tttcatctac
     8941 aacctcaaaa ccatgttttg agctgacttc gtcagttcta tccacaatct caaaacactg
     9001 ttttgagcaa cctgcggcta acttcctagt ttgctctttg attttcattg agtattagtc
     9061 ctttttcaaa cttcctgcac gagtttgggt tcctgcatag gcaagtaaga gaagagttcc
     9121 tgcaatagct acagatacac cattggcaat tcccgcaaca atcccttgtg caaatacttt
     9181 ttctgccgct tcttgataaa tcacaacatc tccaagtggt gccaagacac cccaaacaag
     9241 ggcatttgca agtagttgaa tgaggttaaa aataagaata tctttccagt caaaaacacc
     9301 attgatcact cgaacgtact ttctaaaaag tcccacaact agaccaaaga gtccgctagc
     9361 gataatccaa gtccaccata gaccataacc agcaagagag tccttgattg catgaccaat
     9421 caacccgaca agcaaaccga taatcgggcc aaaaataata gaaagtagcg cttgtaccgc
     9481 atactgaagc tggatgcttg tatttggaac aggggttgga atgttgatca tcccgatgac
     9541 gacaaagagg gcagcgccaa ttccgacagc aacaacttgt ttaattgtaa atttgatttc
     9601 catactattc tcctatttta tccttctatt ttctttattt caatggtcca agatgaaccg
     9661 acacctacat tataggcctt ggcaaaggaa ccttggttga tagccaaacc taaacgatag
     9721 agagagttga tgtaaaggat gggttgccca attctcacat ctgcaaatga tttgccatag
     9781 acaacctgat tttgacagac cagcatatca gcatgataga tggtcacttc aaaacgatca
     9841 ccaaattctg gttccagctt gtaaaattct tcccgtgtga tagaggtcca aagcgaaccg
     9901 aaacgcacat ccagaatatc aatggctccc ttcaccagat gatcttctat gatggtcgct
     9961 acgactggaa gctctacaat ctgttccaca ctgagctctg gccctacttc ctcaaaagta
    10021 atgtgaccac tggccagttt agcaccagta taggcataga catcacgacc gtggaaggta
    10081 taagaatgct ctgtgttttg acgcctattg gccacctcag aaatctcacg aatggctaca
    10141 atgccaacgt gtttcttgat aaaggaaagc gtcccattat ctggcgtgac aatgtattga
    10201 ttttttgcag tcttggcaac tacactctta cgtttcgaac cgacacctgg atcgacaacc
    10261 gatacaaacg tcgttccctc aggccagtaa tccaccgtct gaaagagacg atagctcccc
    10321 tcaaaaatat tataaggcgt gatatcgtgc gtcaagtgat ggatttttaa ggttggagac
    10381 tcttctaaag ccactccaat catagccgat accgcaccat caaccagacc aaagtctgat
    10441 tgtaatacca gtaaattatt attcatttta tctccttgta tatcctttat cataccatat
    10501 ttcagagaaa gtcgctaata gctatttcaa ttggttaggg tatagtttta ccttatagcg
    10561 aattttcact aaaagctctt gttattagct agtaggtgca ttttttcttg aaaaagggta
    10621 tgatagttac atcatgaaaa agtaggtttt attatgaaaa ttatccttgt cggaggggga
    10681 aaagttggtt ttgccctctg tcgctccttg gttgcagaaa agcatgatgt tttgctgatt
    10741 gagcaagacg aagctgttct caatcatatt gtcagtcgct ttgatatcat tggtatcctt
    10801 ggtaacgggg ccgattttgc cattcttgag caagccagcg tccaagattg tgatatcttt
    10861 atcgccctga ctgagcacga tgaagtcaac atgattgcag cagttctagc caagaaaatg
    10921 ggagctaaag aaactatcgt tcgggtgcgg aaccctgaat attctaactc ttatttcaag
    10981 gaaaagaata ttctcggttt ttctcttatc gttaatcctg agctcttggc tgcccgcgct
    11041 atcgcgaata tcattgactt ccccaacgcc ctgtctgtcg aacgctttgc tggtggacgc
    11101 gttagcctca tggaatttgt cgtcaagtcc accagcggtc tttgccaaat gcccatttct
    11161 gattttcgta aaaaatttgg taatgtcatt gtctgtgcga tagagaggga tcatcaaatt
    11221 atcattccaa gcggtgacat gactgtacag gataaagata gaatctttgt cactggtaac
    11281 cgtgtcgata tgatactctt ccataattat tttaaatcac gcgccgtgaa gagccttctc
    11341 atcgttgggg caggtagaat tgcctattat ctacttggta ttctcaaaga tagtcgtatc
    11401 gatacaaaag tcattgaaat caatcctgaa atcgccagct tctttagcga gaaattccca
    11461 aatctctaca tcgttcaagg agatggaacc gcaaaagata tcctgctgga agaaagtgct
    11521 caacactatg atgccgttgc gactctaaca ggagtcgatg aggaaaatct gattacatct
    11581 atgttccttg acagggtagg tgtacagaaa aatattacta aggtcaatcg taccagtctc
    11641 ctcgagatta tcaatgcgcc tgatttttca agtatcatca cacctaaaag catcgctgta
    11701 gatacgatta tgcactttat tcgtggtcga gttaatgccc agtattcaga ccttcaagcc
    11761 atgcaccatc tagccaatgg ccaaatcgaa accctgcaat tccatatcaa ggaagccaat
    11821 aaaatgactg ccaaacctct ttctcaactg aaattgaaaa aaggggttct tattgcagcc
    11881 attattcgaa agggcaagac tattttccca actggggagg atatgttgga agttggagac
    11941 aagctcctag taacaacctt gttgccaaac atcaccaaaa tttatgactt gattgcgagg
    12001 taagaaatga ataaaagtat gattcgttac ctcctttcaa agttactttt gattgaagct
    12061 gttctcctct tggttcctgt ggctatcgct gtctattacc gtgaatcgag ccaagtcttt
    12121 acagccctct tttcgaccat agggattctc gtattgttag gcggttcagg gagtttacag
    12181 aagccaaaaa atcaacggat ttatgccaag gagggagtct tgatcgttgc cctctgttgg
    12241 atcctttggt ctttctttgg tggtctcccc tttgtctttt ctgggcaaat ccccagcctt
    12301 attgatgcct tttttgaaat tagttctggg tttacaacta ctggagcaag tattttgaac
    12361 gacgtttcgg ttctcagccg ttccctcctc ttctggcgaa gttttaccca cttgattgga
    12421 gggatgggag tgcttgtttt tgcacttgct attatggaca atgccaaaaa tagccaccta
    12481 gaggtgatga aggctgaggt tccaggtcct gtttttggca aggttgtatc caaactaaaa
    12541 aacactgccc agattctcta tctcctttat ctagctctct tctccctctt tgtcataatc
    12601 tattatctag ctggtatgcc tctctacgat agttttgtca ttgctatggg gacagcggga
    12661 actggaggct ttaccgtcta taacgacgga attgcccact atggcagctc actgattacc
    12721 tatctggtca gtatcggagt tctggttttt ggggtaaatt tcaacctcta ctactacctc
    12781 atgctccgtc gcatcaaggc cttctttggt gacgaagaac ttcgggctta cttggtcatt
    12841 gtactggttt ctacaggctt gattagcctc aacaccctct acctctaccc agggttttca
    12901 aagagctttg aaatgacctt cttccaggtt tccaacatca ttacaacaac tggttttgga
    12961 tatggagata ttaccaactg gcccctcttc tcccagttta tcctcctttt cctcatggca
    13021 atcggtggct ctgctggatc aactgcaggt ggactcaaga ttattcgagg cctcatcctt
    13081 tcaaaaattg ccaaaaacca aattttgtca atcctatcgc cccaccgtgt tttgaccctc
    13141 catgtgaatc aaacggtgat tgataaagat acccagcata agattctcaa gtactttgtc
    13201 atctatgcta tgattttgct agcgcttatc tttattgtca gcctagatag caatgatttt
    13261 ctggtcgtga ccagcgctgt ctttagctgt ttcaataata tcgggcctat tctaggaacc
    13321 acttctagtt tctcaatctt tagtcccatc tcaaaaattc tcctatcctt tgcaatgatt
    13381 gcagggcgct tggagattta cccaatcctc cttctcttta tgaagagaac ttggtctaag
    13441 agataaaata caaaatccct ctttttacaa ttgtgaaaga gggattctta tattaaaaaa
    13501 gggggaacat ttttaaatcc aagcctatct gtaagcaaaa tccttactca aataaaagat
    13561 ggaaaaactt actacacagc ttggtaatgc tccaatacaa attgtaagga tttacttgga
    13621 ctctttgtta aatgaatata ttccattcct ctggtagcaa ctatctgaat acctaatctt
    13681 tctgcatcca ccttcatctc tctataataa ctccgaactt gtggagccag aataattaaa
    13741 tcataaacac ccataatatc ataatgagct ccgtacgctc ctgaattcgc aatcactcta
    13801 acttctgcta attgagcgcc ctcgttaatt gcattggcta attgcgcact tgttccagac
    13861 cctgcacaaa gaaccagtac tttcaactct ttacttttta tttcatctaa ctcaccaggc
    13921 attatttcac ttgtctcctc ctctaaaata atatcatttg agcttgcaac atcttctttc
    13981 atcagtaact gtctatcata cgctctacag aatggcaaat aaatcaatat gtcgacaact
    14041 aaaatcaaag ataaaaatac aaaggagata gattgaaaat tcgttccaat taacaatccc
    14101 aagggaccag gaaaggccca aggtaactgg atataaaatc cattcattcc aaagaaatca
    14161 ataaagacct ttcctagaaa tacattcact ggtggagtca tcaaaaaagg tacaaaaaga
    14221 tagggattca aaataactgg cataccaaat agaagaggtt cattcaccgc aaataaaaca
    14281 ggagtaattg tagctttacc taccgatttt aattgtttag accgcataaa gaaaatcaaa
    14341 ataaatggta caacaaaggt agcccccgtt cctccgatag cagcgatata atttccgaaa
    14401 ttaggcgtta aagaatgata aggaaactgc ccatttgcaa gaagttgagc attctcttcc
    14461 atattgctca gttgtaaagc tgtaacagca ggtaagacaa tagatggtcc atgaattcca
    14521 acaaaccaaa gcaaagcgca cataaaccaa atcaacatca tagcaggata tgattctgcc
    14581 cccttaaaaa taggagtcaa tagttgttga aatacttggg caaaaggaac atctaaacta
    14641 aaccgagata caatatctaa caaaccacta ataagtaaaa caaaagaaaa aggaaaaata
    14701 tctctaaaag cttgtgatat agccccagga acttccttag gtaaatgaat agtaatgtct
    14761 cgcttaatac agaatcggta aacatttaca gtaataaagg cactgacaaa cgaagttatc
    14821 aatccttgag atcctaaata gtttgtcgac aaacttgtag atcccgtttt ctcatcaact
    14881 acaagcgtta cagttaatac aagatagcca catatggccg ccaacattgc agaagtatca
    14941 tttattacct ttccaggagg catttttcta ttaacatttc cagccaatga ctttgcaact
    15001 gtccctgaaa caataacacc caaaactccc atggttagca tatacacttt cctcatccag
    15061 gcaatagctg gactcggtaa ctctactccg aaatttttag gaatcataat aataatcatc
    15121 aaaaagcttg aaaacataat taaaggcata gtagcaataa atccatcttt aatagccatc
    15181 atataattat tagtagaaat tttttcaaat ttcccttgtt gcttttcaat ggcagcgact
    15241 aacttatcca taaaaactcc tcctgtaaaa aatttaagct catagttaaa atatattaaa
    15301 catcagtcaa atcgctaacc ttaattccgt tttaatcctt ttacccaaaa agcacttttc
    15361 ttaatatatc tcttctgtgt ttcataatca acaaagaaaa gaccatatcg cttattgtag
    15421 ccattcgccc aagaaaattg atcttgcaaa gaccatataa aatagccttg aatattgact
    15481 cccctatctc gagcctctaa aactttgtgt aaatgttgct caatataacg gatt
//
LOCUS       DAVLVZ010000043        14517 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000043 DAVLVZ010000000
VERSION     DAVLVZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14517
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.043"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            695..1971
                     /gene="ltrA"
                     /locus_tag="VYN77_001413"
                     /pseudo
     CDS             695..1971
                     /gene="ltrA"
                     /locus_tag="VYN77_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            2427..3605
                     /locus_tag="VYN77_001414"
     CDS             2427..3605
                     /locus_tag="VYN77_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1558861.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTVYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            3919..4050
                     /locus_tag="VYN77_001415"
     CDS             3919..4050
                     /locus_tag="VYN77_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1558862.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            4071..5621
                     /gene="dltA"
                     /locus_tag="VYN77_001416"
     CDS             4071..5621
                     /gene="dltA"
                     /locus_tag="VYN77_001416"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1558863.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     ITDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDSVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            5618..6862
                     /gene="dltB"
                     /locus_tag="VYN77_001417"
     CDS             5618..6862
                     /gene="dltB"
                     /locus_tag="VYN77_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1558864.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            6876..7115
                     /gene="dltC"
                     /locus_tag="VYN77_001418"
     CDS             6876..7115
                     /gene="dltC"
                     /locus_tag="VYN77_001418"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1558865.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            7108..8376
                     /gene="dltD"
                     /locus_tag="VYN77_001419"
     CDS             7108..8376
                     /gene="dltD"
                     /locus_tag="VYN77_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1558866.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            8531..8971
                     /gene="adcR"
                     /locus_tag="VYN77_001420"
     CDS             8531..8971
                     /gene="adcR"
                     /locus_tag="VYN77_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1558867.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            8971..9675
                     /locus_tag="VYN77_001421"
     CDS             8971..9675
                     /locus_tag="VYN77_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1558868.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVSHA"
     gene            9668..10474
                     /locus_tag="VYN77_001422"
     CDS             9668..10474
                     /locus_tag="VYN77_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1558869.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            10484..11989
                     /gene="adcA"
                     /locus_tag="VYN77_001423"
     CDS             10484..11989
                     /gene="adcA"
                     /locus_tag="VYN77_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1558870.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVTKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYVEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(13117..13890)
                     /locus_tag="VYN77_001424"
     CDS             complement(13117..13890)
                     /locus_tag="VYN77_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1558871.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            14066..>14517
                     /locus_tag="VYN77_001425"
     CDS             14066..>14517
                     /locus_tag="VYN77_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEV1558872.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTN"
BASE COUNT         4349 a         2656 c         3077 g         4435 t
ORIGIN      
        1 gcacctgcta ccggtgaacc caatagcgat tcccaagcct gactatcgtg aggtagcgga
       61 ttaaaacggt ctggggatag accgttttaa gtctgacgca ggaaataaga atcgtcagag
      121 gaagggatag cgaaatcgtg gctctacgaa caggaacgtg atagtaaggc gtatatagcg
      181 gataaggagg cttcaaactc taaagtccaa aaaggtagtc gtaacctata tgtgtaaatc
      241 acgagagtaa ttgaattcgg actaaggttt gtgtgaaaaa gataaatctt tctagagtct
      301 aaagactctg cgtcagattt cctattttca ctgtaacctt ttaacgtcct catatcttgt
      361 ataaacgagg aaagatgtac gacttatccc gtgaggtttc atgagcgctg aaagcgtagt
      421 aacaacgaat catgagaagt cagccgagcc catagtagtg aggaaacttc tgtagtggaa
      481 gtggagcgaa ggggtgaata ctcaaacagt ctggggagag actgtttgag gtctgtcgct
      541 agaaagagaa aacgacagat cgaagtaatc ctacttcact tgtgtctgta aaatgagtgg
      601 tctgatagaa ctggactttg acacgtaatg actagatgaa ggttcaccaa tataagatgt
      661 ccctcaggca ccaaaacaag aaaggaatac gcacatgtca aaactgctag ataagatatt
      721 atcacgcgaa aatatgctgg aagcctacaa tcaagtaaaa tccaataaag gctcagctgg
      781 gattgatgga atgactatcg aagagatgga taattatctc agacaaaact ggcgcttgac
      841 taaggaactg ataaaacaga gaaaatataa gcctcaacca gttcttaaag ttgagatacc
      901 taaaccagac ggaggcatcc gtcaactagg aatttcaaca gttatggata gaatgattca
      961 acaggccatt gtccaagtca tgagccccat ttgtgaaccc catttctcag atacgagtta
     1021 tggtttcaga ccaaataggt catgtgaaaa agccatcatg aagctcttag aatacttaaa
     1081 tgacggctat gagtggatag tggatataga cctagagaaa tttttcgata cagttcctca
     1141 agatagattg atgtccttag tacataacat tatcgaagac ggagatacgg aatccttgat
     1201 tcgtaagtat cttcattcag gtgttatcat taatggtcaa cgttataaaa cactagttgg
     1261 tacaccacag ggaggaaatt tatctcctct cttatccaat atcatgctta atgaattgga
     1321 caaggaatta gaaaagaggg gacttcgatt tgtgcgctac gcagatgatt gtgtgatacg
     1381 gtcggaagcg aggcagccgc taagcgtgtg atgtattcag tcagtcgttt tattgagaaa
     1441 cggctaggtt tgaaagtaaa catgaccaag actaagatta ccagaccaag agagttgaaa
     1501 tatctaggtt ttgggttctg gaaatcatca gatggttgga aaagtcgtcc acatcaagat
     1561 agtgttcgga gatttaagct taaattgaag aaactaacac agaggaaatg gagtatagac
     1621 ctaacaagac gtattgagca actgaatttg tctattcgag gatggataaa ctatttctca
     1681 ttgggaaata tgaaaagtat agtcgccagc atagatgagc gcttgcgtac tcgcctacga
     1741 gtgattattt ggaagcaatg gaagaagaaa tcgagacgat tatggggatt gcttaagtta
     1801 ggagttccta aatggatagc agataaggta tctggctggg gcgaccatta tcaattagta
     1861 gctcagaagt cggtacttaa acgtgctata tcaaaaccag tcctggaaaa acgtggactg
     1921 gtttcgtgtt tggattatta ccttgaacga catgcgttaa aagttagttg aaccgccgta
     1981 tgccgaacgg cacgtacggt ggtgtgagag gggctagaga ttatccccta ctcgattaac
     2041 tcccctgaaa tttattttaa ttatgcaaat ttcacgtatt tttgatgctg agacgacgat
     2101 cctgggaact tttcagatat ttttttgact atctaaatct atcattagaa aagcttagag
     2161 cgccaaagga tttgagcgtt tttctgattt ttaagacttt ttccagtctc tttttcgatt
     2221 gaagatgtaa ttattctact aactaactaa cttcttagta ctagccaaca acgataatca
     2281 taattcctcc taaaattagg aataataaag gcaatagttt ttgttttttc atgtaaaaaa
     2341 cctcactttt gttttctgct attttatgct aaaatattaa aaatcaaatt taattccaaa
     2401 gtttgtaact aaagggggag cgctacatgt ctaattcatt tgtcaagttg ttagtctctc
     2461 aattatttgc aaatttagca gatattttct ttagagtaac aatcattgct aacatataca
     2521 ttatttcaaa atcagtaatt gccacatcac tagttcctat cttaatagga atatcctctt
     2581 ttgttgcgag tcttttagtt ccgttggtta ctaaaaggtt agcgctaaat agggttttat
     2641 ctttatctca atttggaaag actatattat tggcgatact ggtaggaatg tttaccgtaa
     2701 tgcaatccgt agcgcctttg gtgacctatc tatttgttgt tgcaatttcc atactagatg
     2761 gttttgcagc acccgtttcc tatgctattg tgccacgcta tgcgaccgat ttgggtaagg
     2821 ctaattcagc cttatcaatg actggtgaag ctgttcaatt gataggttgg ggattaggtg
     2881 gactcttgtt tgcaacaatt ggtctgttac ctaccacgtt tatcatttta gtcttgtata
     2941 tcatttctag ctttctgatg ttatttcttc ctaacgctga agtggaggtg ttagagtcag
     3001 aaactaatct tgaaattttg ctcaaaggtt ggaagttagt tgctagaaat cctagattaa
     3061 gactttttgt atcagcaaat ttattggaaa ttttttcaaa tacgatttgg gtttcttcca
     3121 ttatacttgt ttttgtaacg gagttattaa ataaaacgga aagttactgg ggatattcta
     3181 atacagtata ctctattggt attataatta gtggcttaat tgcttttagg ctatctgaaa
     3241 agttccttgc tgctaaatgg gaaagtattc tttttcctct tgtagctatg gcaatagtga
     3301 cactgactat tctatatttt ccaaacgcac agatgttttt attattttca gctttagttg
     3361 gtatgttatc gcaactaaaa gaagttcctg aaagtgtatt tcttcaggaa acagtagagg
     3421 aaaatcattt agttaatgtc tattccgttc ttgaagtgat ttctacatta gcattttcag
     3481 tatttgtttt gctaatgagc tatattactg agagttttgg tattagcatc agtttttggc
     3541 tatcagccat ttgtctgatg atagaagcta ttttaattta tatcagacga gattatttta
     3601 aatgagagag tggttaaaaa gtctttaaaa atcagaaaag cgcatagtat caggtgttga
     3661 aaaaacttga tattgtgtgt tttattatgg gaagatttac ttcattgtct cctgaaatgg
     3721 agttttgaag caggatcact ctatttgaaa atatacatag aaaaaccgca taatccttaa
     3781 aataagctga aatctattgg aaacatacgg taaacaattg aaaagatact atataaagtg
     3841 gtacaatggt agaaaaataa gctagtaaga atcactctta ttttagcgaa aaattacaga
     3901 aatgaatggt ttttcttgat gaaacagaga aaagaattgt acctctttct tggtcggaca
     3961 gccttgtatt ttcttatctt tctagggctg ctttacttct ttagctatct tggtcagggt
     4021 caaggaagct ttatctataa tgaattttaa cttgaaggag aatccctatt gtgtcaaata
     4081 aaccaatagc agatatgatt gaaaccattg agcattttgc tcagacacag cctagctatc
     4141 ctgtctataa tgttttgggg caggaacaca cttatggcga tttaaaggct gattcggata
     4201 gtttggctgc agtcattgac caactaggct tgcctgaaaa gtctcctgtg gttgtttttg
     4261 gtggccaaga atatgaaatg ttggcaacct ttgtagcgct gactaagtca ggtcatgcct
     4321 acattccaat tgatagccat tcggccttgg agcgagtttc agctatttta gaagtagcag
     4381 agccaagctt gattattgcc atttcagcct ttcccttgga gcaggtttct acaccaatga
     4441 taaatctagc tcaggttcaa gaagcctttg cccaagggaa caactatgaa atcacgcatc
     4501 cagtcaaggg agatgataat tactacatta tctttacttc tggtacgact ggtaagccta
     4561 agggagtgca gatttcacat gataatctcc tcagctttac aaactggatg attacggata
     4621 aggaatttgc gacaccgagt cgtccgcaaa tgctggcaca gccaccttat tcttttgact
     4681 tgtctgtcat gtattgggca ccgaccttgg cacttggtgg tacgcttttc actcttcctt
     4741 cagtcatcac tcaggacttt aagcaactct ttgcggctat cttttcattg ccaatcgcta
     4801 tctggacatc aacaccatcc tttgcagata tggccatgtt gtctgaatac ttcaacagtg
     4861 aaaaaatgcc tggaatcacg catttctact ttgatggtga agaattgacg gtcaaaacag
     4921 ctcaaaaact gcgcgagcgt ttcccaaatg cccgtatcat caatgcttac ggcccaacag
     4981 aagcgacagt agctctgtca gcagttgccg tgacagacga gatgttagcg actctcaaac
     5041 gcctaccaat cggctatacc aaggctgatt ctccaacctt tatcattgac gaggaaggaa
     5101 ataaactgcc aaatggcgaa cagggagaaa tcattgtttc tgggccagct gtttcaaaag
     5161 gttatatgaa caatcctgaa aaaacagcag aagccttctt tgagtttgaa ggtctgccag
     5221 cctatcacac aggcgatgtg ggaaccataa cagatgaggg cttgcttctc tacggcggac
     5281 gcatggactt ccagattaag ttcaacggtt accgcattga gttagaagat gtctctcaaa
     5341 acctcaacaa gtctcgcttt atcgaatctg ctgtcgcagt accgcgctat aacaaggacc
     5401 acaaggtaca aaatctattg gcttatgtca tcttaaaaga cagtgttcgt gagcagtttg
     5461 agcgagatat cgatattacc aaggccatca aggaagacct gacagacatc atgatgtcct
     5521 acatgatgcc atctaaattc ctttaccgag acagtttgcc actgactcca aatggaaaga
     5581 ttgacatcaa aggattgatt aacgaggtga ataagagatg atggagtttt ttcaacagct
     5641 tcctcattta gagccatatg gcaatcctca gtattttgtt tatgtgattg ctgcaacctt
     5701 gcccatcttt ataggcctct ttttcaagaa acgctttgcc tggtatgaag tgttggtaag
     5761 tctcttcttt attgtcacca tgttggtggg tggaaagacc aatcaactag ctgccttggg
     5821 tatttacctt tgctgggaaa tattgctcct gcttttctac aagcattatc gaaaaagcaa
     5881 ggatggcaag tgggtcttct acttagttag ttttctgtcc ctacttccga ttatctttgt
     5941 caaggtgcaa ccagctatca atggaacgca gtctttgctt gggttcttgg gaatttctta
     6001 cctgaccttt cgttcggttg ggattatcat cgagctgaga gatggagtga ttaaggattt
     6061 taccctctgg gaattcctcc gtttccttct cttcatgcca actttctcga gtggtccaat
     6121 cgatcgcttt aagcgattta atgaaaatta tcaggctatt cctgagcgag atgagttgat
     6181 ggatatgctg gatgaatctg tccgctatat catgtggggc tttttgtata agtttatcct
     6241 agctcatgtt ttaggagaga ccttactacc tcctctgaag aatttagcct tgcagtcagg
     6301 tggcttcttt aatctctatg ccttggcagt tatgtatact tttggtctgg agctcttctt
     6361 tgactttgca ggttattcta tgtttgcttt ggccatctca aacttgatgg gaatccgtag
     6421 ccctatcaac tttaacaagc cctttttatc aagggattta aaggagtttt ggaatcgctg
     6481 gcatatgagt ctgtccttct ggttccgtga ctttgtcttt atgcgaatgg tgatggtgtt
     6541 aaccagaaag aaagtcttta aaaatcgtaa tgtaacctca agcatggcct acattgtaaa
     6601 tatgctgatt atgggatttt ggcatggtgt gacctggtac tatatcgcct atggactctt
     6661 tcatggacta ggcttggtca tcaatgatgc ctgggttcgc aagaaaaaaa cgctcaataa
     6721 ggaacggaaa aaagcaggga aggctgccct acctgagaat cgctggattc agttgcttgg
     6781 catggttgtc actttccatg ttgtcatgtt gtcattctta atcttttctg gattcttgaa
     6841 taatctatgg tttaaaaaat aaaaggaaat aaaatatgga tatcaaatca gaagttattg
     6901 aaattattga tgagttgttt atggaagatg tttctgacat gatggatgaa gatctttttg
     6961 atgcaggtgt cttggatagt atgggaacgg ttgagttgat tgtggagatt gagaaccgtt
     7021 ttgacattcg tgtccctgta acagagtttg gtcgtgacga ctggaataca gctaataaaa
     7081 tcatagctgg tattgtggag ctacaaaatg cttaaacgct tatggatgat cttcggaccg
     7141 gtcttgatcg ctggtttgtt ggtttttctg ctcattttct tttatcctac tgagatgcat
     7201 cataatctag gagctgaaaa gcgttcagca gtggctacta ctatcgatag ttttaaggag
     7261 cgaagtcaaa aagtcagagc actatctgat ccaaatgtgc gttttgttcc cttctttggc
     7321 tctagtgaat ggcttcgttt tgacggtgct catcctgcgg tattagctga gaaatacaat
     7381 cgttcctacc gtccttatct tttaggacag gggggagctg catcgcttaa ccaatatttt
     7441 ggaatgcaac agatgttacc acagctggag aataaacaag ttgtgtatgt tatctcacct
     7501 cagtggttca gtaaaaatgg ctatgatcca gcagccttcc agcagtattt taatggagac
     7561 cagttgacta gttttctgaa acatcaatct ggggatcagg ctagtcaata tgcagcgact
     7621 cgcttactgc aacagttccc aaacgtagct atgaaggacc tggttcagaa gttggcaagt
     7681 aaaggagaat tgtcgacagc agacaatgaa atgattgaat tattggctcg ttttaatgaa
     7741 cgccaagcct ccttttttgg tcagttttcg gttagaggct atgttaacta cgataagcat
     7801 gtagctaagt atttaaaaat cttgccagac cagttttctt atcaagcaat agaagatgtt
     7861 gtcaaagcag atgctgaaaa aaatacttcc aataatgaga tgggaatgga aaattatttc
     7921 tataatgagc agatcaagaa ggatttgaag aaattaaagg attctcagaa aagctttacc
     7981 tatctcaagt cgccagagta taatgacttg cagttggttt taacacagtt ttctaaatct
     8041 aaggtaaacc cgatttttat cattccacct gttaataaaa aatggatgga ctatgctggt
     8101 ctacgagagg atatgtacca acaaacggtg cagaagattc gctaccagtt agaaagtcaa
     8161 ggttttacca atatagcaga tttttctaag gacggtgggg agcctttctt tatgaaggac
     8221 accattcacc ttggttggtt gggttggttg gcttttgaca aggcagttga tcctttccta
     8281 tccaatccca caccagctcc gacttaccat ctgaatgagc gctttttcag caaagattgg
     8341 gcgacttatg atggagatgt caaagaattt caatagataa tataatatag aagagttcaa
     8401 gaatgaaact agttttcacg ttttttcttg actctttttt tgcttgtgat ataattaact
     8461 ggtaaacaaa atgtcaaaga atagtatttt tctcttaaaa aagagaagtc taaaaggaaa
     8521 ggagtcagat atgagacagc tagcaaagga tatcaatgct tttttgaatg aggtgatttt
     8581 gcaggcggaa aatcagcatg aaatcctaat aggtcattgc actagcgagg tggccctgac
     8641 caatactcag gagcatatcc ttatgctctt gtcagaggaa tctttaacaa attcagaatt
     8701 ggcccgtcgt ctcaatgtca gtcaggcggc agttaccaag gccattaagt ctttggtcaa
     8761 ggaagggatg ttggaaacat ctaaagattc taaagatgcg cgtgtgattt tttatcagtt
     8821 gactgacttg gctcgtccaa tcgctgagga gcaccaccat caccatgagc atacactttt
     8881 aacctatgaa caagtggcga ctcagtttac tccaaatgaa caaaaagtga ttcagcggtt
     8941 tttgactgct ttagtaggag aaatcaaata atgagatata ttacggtaga ggatttgtcc
     9001 ttctattatg ataaggagcc tgttcttgaa catatcaatt atagtgttga tagtggggaa
     9061 tttgttacct tgactgggga aaatggagcg gctaagacga cgctcatcaa ggccagtctt
     9121 ggaattctgc aaccacgcat tggaaaggtg gctatttcaa agacaaatac gcaaggtaag
     9181 aaattgagaa tagcctatct tcctcaacaa attgccagtt ttaatgctgg ttttccaagt
     9241 acggtctatg aatttgtcaa gtcgggtcgc tatccgagaa aaggctggtt ccgtcgtttg
     9301 aatgctcatg atgaggagca tatcaaggcc agtctggact cagttggcat gtgggaacat
     9361 cgagacaaac gcttggggtc tctatctggg ggacaaaagc agcgagcggt aattgcgcgt
     9421 atgtttgctt ctgaccctga tgtgtttatc ctagacgagc cgacaacggg gatggatgca
     9481 ggaagtaaaa atgaatttta cgaactcatg caccacagcg cccatcatca tggcaaggct
     9541 gttttgatga ttacccatga ccctgaagaa gttaaggatt atgcggatcg caatattcat
     9601 ctagtccgta accaagactc gccatggcgt tgtttcaacg ttcatgagaa tggccaggag
     9661 gtgagccatg cttagtttat tatcttacga ctttatacaa cgcgcctttt tggcggttat
     9721 tgctatgagt cttttctcac cggtattggg aaccttcctc atcttgcgtc gtcagagttt
     9781 gatgagtgat acccttagcc acgtctcact ttcaggtgta gcctttggtc tggttttggg
     9841 gatttctcca actgtttcta ctattgccat tgtcttgatt gcggcggtct ttctggagta
     9901 tctccgtacg gtttacaaga gctttatgga aatcgggaca gctatcctca tgtcaacagg
     9961 tctggctgtt tctctgattg tcatgagcaa gggtaaaagc tcgagttcaa tgagtttgga
    10021 ccaatatctc tttggttcga tcgtgactat cagtgaagaa caggtcattt ccctctttgt
    10081 cattgcggcg gttgttttga ttttgacctt tctctttctt cgtcctatgt atatcttaac
    10141 ttttgatgaa gatacggcct ttgtggatgg cttgccagtt cgtaccatgt ccattctttt
    10201 taacatggtg acaggggtgg ctattgccct tatgattcct gcagcaggag ctcttctggt
    10261 atcgaccatt atggtcttgc cagctagtat tgccctgcgt ctggggaaaa actttaaatc
    10321 ggttatgctg cttgccagtg cgattggctt tttgggaatg gtagcaggac tttacatttc
    10381 ctactatgca gaaacacctg caagtgcaag tattaccatt atttttgtaa ctgtctttat
    10441 actaatcagt ttagtaagac gttttatcaa ataggagacg aacgtgaaaa aaattagctt
    10501 attactagcc agtctatgtg ccttgttttt agtggcttgt tccaatcaaa aacaggcaga
    10561 tggtaaactc aatatcgtga caacctttta ccctgtctat gaatttacca agcaagtcgc
    10621 aggagatacg gctaatgtag aactcctaat cggtgctggg acagaacctc atgaatacga
    10681 accatctgcc aaggcagtta ccaaaatcca agatgcagat accttcgttt atgaaaatga
    10741 aaacatggaa acatgggtac ctaaattgct agataccttg gataagaaaa aagtgaaaac
    10801 catcaaggcg acaggcgata tgttgctctt gccaggtggc gaggaagaag agggggacca
    10861 tgaccatgga gaagaaggtc atcaccatga gtttgacccc catgtttggt tatcaccagt
    10921 tcgtgccatt aaactagtag agcacatccg cgacagcttg tcagcagatt atcctgataa
    10981 aaaagagacc tttgagaaga atgcagctgc ctatatcgaa aaattgcaag ccttggataa
    11041 ggcttacgta gaaggcttgt ctcaagcaaa acaaaagagc tttgtgactc aacacgcagc
    11101 ctttaactat cttgccttgg actatggact caaacaagtc gcaatctcag gactttctcc
    11161 agatgcagag ccatcagctg ctcgcttggc agaattgaca gagtatgtca agaaaaataa
    11221 aatcgcctat atctattttg aagaaaatgc ctcacaagcc cttgctaaca cactttcaaa
    11281 agaagcaggt gtcaaaactg atgtcctcaa tcctttagaa agtctgacag aagaggacac
    11341 caaggctgga gaaaattaca tctctatcat ggagaaaaac ctcaaggctt tgaaacaaac
    11401 aacagaccaa gaaggcccag caatcgagcc tgaaaaggca gaggatacca agacagtcca
    11461 aaatggttac ttcgaggatg cagctgtcaa ggaccgcacc ttgagtgact atgcaggtaa
    11521 ctggcaatca gtttatcctt tccttgaaga cggcacgttt gaccaagtct ttgactacaa
    11581 ggctaagttg actggtaaga tgacccaggc tgagtacaag gcttactata caaaaggcta
    11641 tcagacagat gtgactaaga ttaacattac tgataatact atggaatttg ttcaaggtgg
    11701 acaaagcaag aaatacactt acaagtatgt cggtaagaaa attttgactt acaagaaagg
    11761 caatcgtggc gtgcgtttcc tctttgaagc cacagatgct gacgctggac aattcaagta
    11821 tgttcagttt agtgaccaca atatcgctcc agttaaggca gaacatttcc atatcttctt
    11881 tggaggcaca agccaagaag ccctctttga agaaatggac aactggccaa cctactaccc
    11941 agataaccta tctggccaag aaatcgccca agaaatgttg gcgcattgat gagaaatcga
    12001 ctagttcata agagctagtc ggttttttga tactagatgg ctaacttcat tatagacctt
    12061 tcagaactaa gggaacctga aaaaataaat aaaactcttg aaatattagg acttatatgc
    12121 tagaatgtag ttagtgtata aggttcatta gaacaccaaa gcccctaact agtgccatag
    12181 ttaagggctt ttttgacgta tcttagtgat ttaacgggtg tttataggct agtcagtcgg
    12241 tctacttctt accatctagc catttgcaga cgaaatacaa gataattcca gctataacat
    12301 ctgctataat agtaagagca agctctggta taaggctcat tagtttcacc tcctctcacg
    12361 aacccatagg aacgtaatcg gtaaccgatg acaaaaatag tataccacac taaatttaga
    12421 tcatcaacct cacttaatgc ttgaactatt gtagattata tgctaaaatg tagttaattg
    12481 ataaggttca ttagaacacc aaagccccta actatggccg tagttaaggg cttttttgac
    12541 gtatcttaga gatttaacgg gtgtttatag gctagtcatt cggtctactc cttactatct
    12601 agccatctgc agatgaaata caagataatt ccagctataa catccgctat aatagtaaga
    12661 gcgagctcta tgataaggct cattagtttc acctcctctc acgaacccat aggaacgtaa
    12721 tcggtaaccg atgacaaaaa tagtatacca caatacattt agatcatcaa ggtcacttaa
    12781 ttcttgaaat atagggtcta ggcgacaaag tctttaaaat cagaaaaacg cataatatca
    12841 ggtgtgcaaa aacttgatac tatgcgtttt attgtgggaa tatttgcccc atttctcttg
    12901 aaattgagtt tttgtccagc ctctgttttt agggttgcta agaaaataat gtcatgtggt
    12961 gaatatttgt aaatcagtca gcagacagaa cgagtattct tcgaaaatct cttcacatca
    13021 tgtcagcttc gtcttgccgt gtccatgggg gacttgactt catcagtttt caaatctgta
    13081 ctttgagcaa gctgagacta gcttcctatt tgattttcat tgaatatcag aaacccattc
    13141 tccatcaaat aattcgactg cgtctaataa tttttgatct ggcacggtgt cagaaataaa
    13201 ggttgtgtat ttggataggg gattaatttt aaaaaatcca gtcttgtaaa atttagagct
    13261 atcaatcagt aagatggttt catgggcttt gtcaataata ttcttttttg aaatagcttg
    13321 gctgagagaa gcttcataaa catattggtc atcaatacct cttgctgaac aaaatgctaa
    13381 atcgatatta aaatgatcta ataaagaatt ttccttatcg taattgacca cggaacagga
    13441 ttgatgtttg acctcgccag atgtgataaa gattttggag ctatctttaa cagtttcaga
    13501 tagggtttgt gcagtatgta aaccatttgt aaaaataatc aaattatcaa gttcagaaag
    13561 atagggacaa agttcgtaga cagtcgtact ggaatccaga taaacacaca tacctgaccg
    13621 aataaagtct ttagcgagac tagcgattag tcttttttgc ctagtacttt ctccttcccg
    13681 tatttgatga gaaagttcaa ttgtgttcat agaggacagg gtcacgtatc cgtgctttct
    13741 tttgataaga ccttgatttt ctaagaaaat taaatcacga cgtaaggtac ttgtgctgga
    13801 gaaagtgatt tctgccagct cttttacggc aattcttttt ttctttttaa taatttcaat
    13861 caattcaagt acacgttcat cttttatcat aagctcctcc taatttatca tttcaactat
    13921 attatagcac aaattggagg aatttgaatt atttttatga atattgggtt aacatttgaa
    13981 cattatccaa gtaagcgttc acatattgaa aaaataaaat gtggggatta taataaactt
    14041 aatcaaggac gaagagagaa gaaaaatgga agcggtttta gcaatagatt taggtgcgac
    14101 ttctggaaga gcaatcgttg gttacctttc tgaaaataaa ctagtaatgg aagaaataaa
    14161 tcgcttttct aatctaccta ttagagtaaa agggcattta tcttgggata ttgactttct
    14221 actagctaaa attcttgaaa gtatccgctt ggctaatact agttacaaga ttttatctat
    14281 cggtattgac acatggggag ttgattttgg actgattgat aatgaaggta agctgttatc
    14341 acaacctgtt cattatcgtg atgaaagaac aaagggagtg ttaaaggaaa tatctgaaat
    14401 gactgaatta gaaaaactgt attcagagac aggaaatcag attatggaga taaatacctt
    14461 gtttcaactc tttaaggcac gtcaagaatc tcctgactct ttctataaga ccaataa
//
LOCUS       DAVLVZ010000044        14477 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000044 DAVLVZ010000000
VERSION     DAVLVZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.044"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(1..297)
                     /locus_tag="VYN77_001426"
     CDS             complement(1..297)
                     /locus_tag="VYN77_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558873.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(281..2440)
                     /gene="pfbA"
                     /locus_tag="VYN77_001427"
     CDS             complement(281..2440)
                     /gene="pfbA"
                     /locus_tag="VYN77_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1558874.1"
                     /translation="MLKIVKKLEILMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIIFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRENGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3571..4797)
                     /locus_tag="VYN77_001428"
     CDS             complement(3571..4797)
                     /locus_tag="VYN77_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1558875.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKIVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWRANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4840..5532)
                     /locus_tag="VYN77_001429"
     CDS             complement(4840..5532)
                     /locus_tag="VYN77_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1558876.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLAFVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(5705..7456)
                     /locus_tag="VYN77_001430"
     CDS             complement(5705..7456)
                     /locus_tag="VYN77_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558877.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(7446..9191)
                     /locus_tag="VYN77_001431"
     CDS             complement(7446..9191)
                     /locus_tag="VYN77_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1558878.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYTTSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9397..9774)
                     /locus_tag="VYN77_001432"
     CDS             complement(9397..9774)
                     /locus_tag="VYN77_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1558879.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVALKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYK"
     gene            complement(9846..10302)
                     /locus_tag="VYN77_001433"
                     /pseudo
     CDS             complement(9846..10302)
                     /locus_tag="VYN77_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10435..11262
                     /gene="xth"
                     /locus_tag="VYN77_001434"
     CDS             10435..11262
                     /gene="xth"
                     /locus_tag="VYN77_001434"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1558880.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11274..11417
                     /locus_tag="VYN77_001435"
     CDS             11274..11417
                     /locus_tag="VYN77_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1558881.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCDLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            11528..11656
                     /locus_tag="VYN77_001436"
     CDS             11528..11656
                     /locus_tag="VYN77_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEV1558882.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHEFAVLDPNGYFLRFSE"
     regulatory      12039..12134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12534..13115
                     /locus_tag="VYN77_001437"
     CDS             12534..13115
                     /locus_tag="VYN77_001437"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1558883.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFATTGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIKIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13115..14377
                     /locus_tag="VYN77_001438"
     CDS             13115..14377
                     /locus_tag="VYN77_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1558884.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIAMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIFVASLLAIVLNAVLNHKKK"
BASE COUNT         4269 a         3088 c         2728 g         4392 t
ORIGIN      
        1 ttatttcatt ttactatact ctgttaattc atatgagttt aaaccgattt catctttaac
       61 ctcgagtaaa gcagtttcaa atatttgttt aagagttttt gattctttac aattaaccga
      121 caaactttct gataaaatat gtacaacttc tgagactgaa taacctatct cctctttaga
      181 attatataaa tctgtagctc caccaataat ccaaaaatac tgattttgtg aacctacaat
      241 atcctcattt tctacggaac ttccttgtat cgaacaaatt ttatttatct ttaccataat
      301 acttcaaccc ttttagtgtc aaaagtaaac caattcctgt cactgttaag aatagttcca
      361 taatcttgtt cgaaccagtc tttggtaatt tttgttttac atctactatt tctttagatt
      421 tattaatatg attttcagtt tctctgccat ctccaactat tttatagttt acttcttctg
      481 tcttattatc ttgtttattg tcgatcttgt cattcatttg tctattatct ttacttgagt
      541 taaactctcc gttcttctgg ttactatcaa ttacattatt tgaattagat tgtttttcct
      601 ctttgttttt ttctttttcg tttttatcac ttaaattatt tgttacaatt ttgtaaagcc
      661 cattctccgt tacaatattg aaattaccat cgctatcacg tataacaggt tctttcccat
      721 ttgcattaga tttgatgaat gatatatact taccggataa attataaaat tggttattta
      781 aaacggttat tttacccttt gaatcctcaa taacaattcc ttctttacca ccgaatatac
      841 tattctctga gactactaaa ttatcactaa ctcgtaataa ttcaatattt ggttgttctg
      901 tttccttaga attattatta attacatttt ttgttacagt aatatctgat acttttccta
      961 aatattctgc actatctttt gcaactcgta tcgcttttgt tttaggatcg gcaatattaa
     1021 atatattttc ggcgataacc acctgtttat ttaaatctaa taggtcttta gtgtttttat
     1081 agctataagc atttactaaa gctgctccgt tttctcgata atgtacactc tctcctttaa
     1141 ctttcttatc aaagcgattt cctttgatta atacatcagt gaatcctgta aaacgtacac
     1201 ctgcatacat catgttatca aaatgattat ttaaaatttt aatattagag gggttctgtg
     1261 tcgacaatgt ttgatagtgt gtgccaattg ctgttactaa ttccccagat ttatcacttt
     1321 tgccaaaata ggaattttga atagtcacat tttcagattt tttcccatca tcattcaagg
     1381 cataaggaaa accttttcta gttaatggtt caatctgaat gctctcctta cttatgattt
     1441 gcccatcctt catcgttttg ggtaaggctt gcccaagaaa acgagaatta tcaactaata
     1501 catttttcga acctgcaatt tgaatagcat gcccttgata actatccttg aatgttacat
     1561 tttttatagt tacgttattt gaattcccaa tagcaaatgc acctgaagaa tttataagtg
     1621 gtagattttt tgctttagtt ccttcttcat tcaaagcacc gttcatatca atcgtaccac
     1681 cagaataact aatatcttct gttgggcccc attctacttg cgcaccatca tccgtaaata
     1741 aacctgtcat aaaaacaatg gaagggtgat tcttaatatt tataccattt agaattgtag
     1801 ctttctcatt caattctaag tgtgtatgac tttttaaaaa aataatttct tttactaaat
     1861 aagttccttc aggaaaatat acatttcctc cacctagccc ttgagctgca gcatctattg
     1921 catcttgaat tgcttgtcga tcatcatgaa tcccatcacc taccgcacca taatctttta
     1981 cacttaaagc agtatcttca aattcttttt tgataattct gtcacttata acatcttcct
     2041 tataactctt attttcatta gtagatgtta ctgtagattc aactgttctg ttgtctaatt
     2101 gtgaattgga acgaatagta ccattagagc ttatactatt aacacttaac gaattatcta
     2161 aaccattagt ggttgaatta gaggcaatca tttcagcaga acgattctga ttaacagttg
     2221 gtgaattatc taaatcatta gtaattgcat tagaagactc ttttgtagcc atcggtgaag
     2281 aactagtaac aacttcatcc gataaaatac cttggcttcc caaaattgaa attgccaata
     2341 gtaccgagca agtccccacc tttaatttac gaatactgaa tacctcatta ggaacaaaat
     2401 atttcatcag aatctccaat ttttttacta tttttaacaa ttgattatta cttttcttaa
     2461 aatagataga atgacaccgc tgtcattatc tttatcatat attttccaaa ttgaattgtc
     2521 aaggaataac acaagaaaat attaaaattt atactcttcg gaaatctctt caaactaggt
     2581 cagtgccgcc ttgccctaag tctaggttac tgacttggtc agttctatag tagactgaaa
     2641 ctaaaatagt acacctctac ttctaaaaca ttgttagaaa tcgatttgac tgtcccgatc
     2701 aatttgtcct attcttattt cattttacta tatctacaac ctcaaagcag ggttttgagc
     2761 tatctatggc caacttccta gtttgctctt tagccttcca ttcaaaagtg atgctatctt
     2821 tcttagcttg aatataaaat atcccattta ctatatttaa atttattgat atgttgtaaa
     2881 ttataaatga aagaacaaat accttgtcat acaagcattc gccggggata tttacctgaa
     2941 tatcgctcat atggcggatt gaaactaaaa cactacaata tatttcttaa atagaccttc
     3001 tataaaagct agatttaacc tcgataaaaa tagatgcagt gcttctaaaa tactggaata
     3061 gggcatatag gtaatcgtag acaagtgttt tatagattgt ctcgcgggag atctaatgat
     3121 gtatagcaag catcactaaa catatgggat attcgtatta ttataatatc aacatgctat
     3181 aaattctatc atgcaaaaga atgactagaa catgaaagac aggcacaaaa ctttgaacaa
     3241 tattctgaaa acaaaaaaaa gacgcgagtt tccacaatct cactcatgga aactcacgtc
     3301 tttcaaaaaa tagggtgaat tgaaaactag aacaagacac tagattttct aaatgatgtc
     3361 tagtaaggat tctaaatgct acaccaaatt gctcacatct tatttcaaac cacgggactc
     3421 ccacaagaaa ggaaaatcct ataaaaattc tctctctgag gagaaagagg cctcaactac
     3481 aggtgtaaac tattatcaat tgaaaacaac agtttttaca ctcaattaga ctttttaggg
     3541 acaggatcac tttcgcttga ctgtagtttg tcattttttt gataaagtta gcactttttc
     3601 agaaactgtt ttgaaagtct caatgatata gtctacttct tcatcgctta atttagtatg
     3661 aagagggagg gtaatttcat tctcaaagaa ggcataggcc ttaggatagt tcgtcatatc
     3721 aaatccaaga ttcttatagg ctgtcaagag aggaagcggt ttgtagtgaa cattacttgc
     3781 aattcctgct ttagccaatt cttggatgat gaggttgcgt tcttctaggc ttgctccttc
     3841 tacacgggtg atgtagaggt ggcgtgaaga ttcgacagtt tcagtcttgt gtgccaaagg
     3901 atggatgcga gaacctgcaa aaccactatc atagcggtcc acaatgtcct tacggcgttg
     3961 caacaaactt ggatagcggt ccaattgtac caaaccaagt gaagccatga tatcggtcat
     4021 gttgcactta taggctggtg taacgatatc gtattcccat gaccccagtt gcatcttggc
     4081 aagagcatcc ttagtttgcc cgtgaaggga aaggatttgg aattccttgt acatctcttc
     4141 gtcatcaatc actggattgg ctctccaagt cgcacttcca ccttctgccg ttgtaaagtt
     4201 cttaacggca tggaatgaga aggaagtaaa gtcagcgata gaaccagaag gttgtccttt
     4261 ataagtagat cccaaagcgt gggcactatc agagacaatg acaatacggt taaaggcctt
     4321 ttgccacttg cttgaagcgg taaagaagtc acgttttttc tccacgactt ggaacaaacg
     4381 gtcataatcg caaacaatcc ctgcgagctc tactgggata atcaccttag ttttctcagt
     4441 gatagcttgc tcaagcaggt catagtccat ctcaaacgta tctgcttgga tatccaccat
     4501 gacaggggtt gctcccacgt gcgtaatgac actacatgaa gccgtatagg tcatggctgg
     4561 aacgatgact tcatcaccag gtcccacttc caaaacgcgt aaaatcaact ccagagcagc
     4621 tgtcgcagag ttgagacaaa caatcttagg tgtctgtgtg taaagagaca agcggcgctc
     4681 cagttctttt gttttaggac ctgttgtgat ccaaccagaa cgcagggtat ccgctacttc
     4741 agcaatttct gcttctgtga tatcaggcgg tgaaaatgga atattgtaat ttggcattga
     4801 tttgctcctt ttatctgtac tcattttctt atgactactt tattttagta cctcaaacac
     4861 ggtttgaaac atgattttga tgtccccaaa gaaactaaac tctcggagat aggcgaggtt
     4921 atagcgcatc ttttcaggaa gaacatgctc cacataggcc tgatcaactg acagaccttt
     4981 ctccgtcatt tgactgatga tggtgtcctc atccttgtag ttgatgctgg ctggagaggt
     5041 aattcctgct tgcaagagca aggttgccat catttcaggg ctatactgct ctgtataacg
     5101 tggcacttca ggtcgtgtac cgacaaagga catctcacct ttaaggacat tgaccaactg
     5161 aggcagttcg tccaaacgga cacgtcggat gaaatttcca accttggtaa tacggctatc
     5221 gttagcagaa gtcaccagac ttcctttttt atccgcatcc gtcaccatgg tacggaactt
     5281 ccaaatcttg aaccgacggt tgtactgggt cacacgctct tgcttgtaaa tcactggccc
     5341 cttgctatcc aacttgatcc aaatgctcaa gatgagaaag atgggagagg tcagaaccag
     5401 taagacaaag gctagaaccc agtccaagca acgcttgaaa atcagcgaac ccttcctttt
     5461 agagacaagc tggtaataag actcaacctc gcttgatttc atttccacag gcaagtcttc
     5521 ccatttcagc atactgttct cctttgtttt tattatacta tttgtcaaca tttttcatta
     5581 taccacaaaa tggaaaaggc ggtgaaagaa atctgtaatt tgaaggattt ccttctagta
     5641 ctcaatgaaa atcaaagtac agctttgaga ttgcagataa agctgacatg gtttaaagag
     5701 attttcaaag agtataggcc atggcccctg cctgcaaact atacatcttg tgataggttc
     5761 ctcccagagt caagagttcc tcatgggttc cactctcgat aatgcgtccc ttatccaaga
     5821 catagatgca gttggcgtct tgaatagtag aaaggcggtg agcgatagca atagttgttc
     5881 ggccctgtct catcttcgcc agagaagctt gaaccaagct ttctgtttca gagtcaatat
     5941 tggctgtcgc ttcatccaaa atcaggattt taggctggct ggcgactgtt ctagcaaagg
     6001 caagaagctg gcgctgccca gtagagaagc tcgaaccacg ctcggaaaca ggggagtcgt
     6061 acccctgagg aagttcttga ataaaggaat ctgcatccac aaaggctgcc gcagcctgaa
     6121 cctgctcatc actgatttct tggtacatgg cgatattgga cttaatagtt ccatgataga
     6181 ggaagggttc ctgtaagacc aaaccgatgt tttttctcag ctcttcttga ctgaaatccc
     6241 tgatatccac atcatccaag agaactctcc ctgactggaa ttcataaaag cgcatgagga
     6301 cattgataat agacgatttc cctgaacctg tatgacctac aaaggcaatg gtttcaccct
     6361 tattaacaga gaaagaaatg tcatccagaa tcggatgttt accgtcatat gagaaacaca
     6421 catgttcaaa acggatattg ccttcttgga ctttggcttg cccattttct tgaagaggtt
     6481 cataggtcct ctcgtctatc agggcaaaga cacgacctgc agaaaccata gccgtttgca
     6541 gagttgaaaa gttttgcgtc acctcaatca aggggtcaaa aaggcggttg atgtactgga
     6601 taaaggcata catggtcccg accgttatcc cgatagaaaa accacggtag ccaaagtagg
     6661 ccatcaagac tgcatagcct agaagtttca gcaaactcat ggcaggtctc aaaaagaggg
     6721 catccaaggc tacagaacgg ttggcgtaga ccaagtgttc ttggttgatt tcatcaaatt
     6781 ctgcctgcag gcgcttctct tgattaaaag cctgaataat cctgattccc tcgatattct
     6841 ctgccagctt actattgata tctgacaaga gacttctggt tttctcgatg attttcactg
     6901 acttttttcg atagagattg accaaaagga aaatcaaggg gagaaagagc aagactaatg
     6961 ctgtcaaacg aaaatccagc accaacatgg tataaagggt tgtcagaaag ataaaaactg
     7021 ctgagataaa gctggataaa atcccagaaa acatatcact aatcgtctcg gtatcgttgg
     7081 tcaaacgaga aacgatagaa cctgctggcg tcttgtcaaa gtaagacatg cccagtttct
     7141 ccatattggc aaaggcatcc cgacgaatat ccctaacaat actgtaagac acgcgcgcaa
     7201 agagaagatt gccgacatac tgaactacag tttgtaggat gtagagacca tagtagacca
     7261 gcaaaacggt cacggctagt tggttaagat tgctgagata ctggtcgata aagtgggaag
     7321 ccacaagggg aatgacactt ttaatgaccg tcgtcgctag gagaaaactg agtgccaaaa
     7381 aggtaaggag tccataaggc ttgagatagg acatcaagcg cttcaataca gtccattgtt
     7441 cttgtttatt ctgcatcttc ttctcccttc atttccagct gctgagactg gtaggtttgg
     7501 gcataccagc catccaaagc tagcaagtct tcgtgcctgc ctcgttcgat aatttgacca
     7561 ttttgcagga ctaaaatcaa atctgcatgg acaactgcac tcaggcgatg ggcagtgata
     7621 atggttgtct tgtcctttcg cgtctccttg aggttgtcga taatcgcata ctctgtcttg
     7681 gcatctacgg cggataagga atcatccaaa atcaagatat cagggtctaa aatcatagcc
     7741 cgactcatag ccaaccgttg cttttgacca ccagaaagac tgactccttt ttcaccaatc
     7801 agcgtatcaa atccttgagg catgtctaca atatcttggt aaactcgggc tagcttagta
     7861 gcttcctcga ccgctgaaag gggcaagtta ggattgccaa agcggatatt gtctaggatt
     7921 gaagtcgcaa aaagaaactg gtcctgagga acatagccca tgagactgcg aaggtctgtc
     7981 agacgatagt cccgaatatc gtgaccgttt agataaatgg cgcccttatc cacatcgtat
     8041 tcacgcaaga ggagcttgat taaggacgtt ttcccagagc ctgtctgccc aaccaagccc
     8101 agtgtttgcc cttttgccaa actaaagtga atatccgtca gtgtttcctc attttcaaaa
     8161 gcaaagctgt caatggcata ctccaaacgc ccattttcaa taccatccag aggaaactcc
     8221 gggtcttgta caggagattc ctgagacaaa agattttcaa tccgctggta agaaaccttc
     8281 cctcgctgag tagtattaaa gaggaagccg atggccataa gaggccagac cagcatatcc
     8341 aaatagctga taaaggtgac tagattccca actgtaatct gcccttcctg aaccatcaag
     8401 gagccaacca aaagcgttaa aacataggac gaaccaacaa acaagagaac catagggtca
     8461 aagagactat catatttcat ggtttgcagg ttcttttgga aggttaattc attgactgcc
     8521 tgaaaagact tcaactcgtc tgcctgataa ccgaaagact tggtcacttt gatacctgat
     8581 acggactcct gtaccttgtt attgagttca gaaaaagcag cttgggattc gccaaaggcc
     8641 ttatgagtct ttctccctag gcgactagtc gtataggcca tgaaaggtag ggggagaatg
     8701 gcaacaagag tcatctgcca tgagatgcta aagagcatgg tcaacaaagt caccagagcc
     8761 gtgatagagg catccaccgc agacatgaca ccgccacctg ctaaacgagt caaggcattg
     8821 atatcattgg ttgcgtgtgc catcagatca cccgtccgat aggtttgata aaaggctgac
     8881 gacatttttg tgaaatgctt aaacaagcga gaccgcatga tctgtcccaa gcaataagag
     8941 gtcccaagga tatacatacg ccacacatag cgcaaatagt acataccaaa ggctgcaagt
     9001 agcaagtaaa ataggctaag aaggaggtcc tgctgagtta attgccccga tgtgatggca
     9061 tcaatgactc gccccataac cataggagga atgagattga ggacggaaac caagaccagg
     9121 gccacaatcc cgactagata acggcgtttt tctaacttga aaaaccacca gagtttttga
     9181 ataatggaca taaaatccct ttctgattgc aaatggaaac ctgaggccaa tactcaatga
     9241 aaatcaaaga gcaaactagg aaactagccg caggctgctc aaagcactgc tttgaggttg
     9301 tagatagaac tgacgaagtc agtaacctac atacggcaag gcgacgttga cgcggtttga
     9361 agagattttc gaagagtaca agaccccagg tttttcttat ttataagtta cgactgtaac
     9421 agcacccttg tcatattcag caataaagat attggctaca ttgtcatgcc cttgtttact
     9481 gaggttatca agcaaccact cttcgctacg accaatcgat tccaagacat ctacttgaat
     9541 cacaccgtca gtcacaactg gatacttagg attttcatct cccatttgca caacgatgag
     9601 ttgcccattt tgctcttgca cagctcgttt gacttgcttc atctggaaaa tcccttggct
     9661 acgaagtttg agggctacat ccgatgcaga caaaccaact gaacgacagg cttctgggtc
     9721 aatattccca tttttgataa ggagagttgg cttaccatca atcaaacgtt tcacaaagtg
     9781 aacattgttg ttgagctatt tgagagtcaa gaccagtttg attaaaattt cgatataatt
     9841 aagtgtcatt tgttcacatc caccagttca attgcgtctg tcttatacaa ttcgactttc
     9901 tctaacaggt acttgtctgg ctcacttccg tttagggcac gataatagcg atctcccacc
     9961 ttgataagtg cgccatctgt gtttgtggaa gtattaacat agacttctga cttgtctact
    10021 cccaaatctt tggaaacaac ctcgatgaaa tgaagtgaag tttgaaattt attgttagaa
    10081 acttgattgt tttgataagt cgtgatgctg accaaaagca gagctaaata aggccagagc
    10141 tgaaatcatg accaactcac gaaatttggt tcctttttta tcatggtatg ccttaaaagc
    10201 aaaaaaaact gtcacagcta atagaacaat cccaaagcca accatgatac cattttgctg
    10261 accgatttgg ctgagtacat agtcataaga gtagaatttc atgtattttc actccctttc
    10321 ataaaatcat tcttaattat aaacgaaaga gagtaatttt tcaaaccata cgttggggaa
    10381 atagatcttt ttttggtata ataaaatcta taatctgaat gaaaaggtaa ctttatgaaa
    10441 cttatctcat ggaatattga ttccctaaac gctgccctaa ctagtgactc agctcgtgcc
    10501 aaattgtccc aagaagtcct acaaaccttg gtcgctgaaa atgctgatat cattgctatc
    10561 caagaaacca agctttctgc caaaggacct acaaagaaac acgtggaaat tttagaagaa
    10621 ctcttcccag gctacgaaaa cacgtggcgc tcttcccaag agcctgcccg taaaggctat
    10681 gctggaacca tgttccttta taagaaagaa cttacgccta cgatcagctt cccagaaatc
    10741 ggtgcccctt ctaccatgga cttggaaggc cgcatcatca ctctagaatt tgatgcattt
    10801 ttcgtgaccc aagtttacac accaaatgct ggcgatggtc ttaaacgctt ggaagaacgc
    10861 caagtctggg atgtcaaata tgcggagtat ttggctgaac tagacaaaga aaaaccagtc
    10921 cttgcgaccg gtgactacaa cgtagcccac aatgaaatcg accttgcaaa tcctgctagc
    10981 aaccgccgtt cacctggatt tactgacgag gaacgtgctg gatttaccaa cctattggca
    11041 actggattta ctgatacctt ccgccatgtt catggcgatg ttcctgaacg ctacacttgg
    11101 tgggcacaac gcagcaaaac ttctaaaatc aacaatacag gctggagaat cgactactgg
    11161 ctcacaagta accgtatcgc tgacaaggtc actaaatctg atatgattga ctcaggtgct
    11221 cgccaagacc atacaccgat tgtcttggaa attgatttgt aaggattttc tatatggact
    11281 acaatgcggt cattcccgag ttgcttgtat cgaatatcga acagtcccgg tccttctact
    11341 gtgatttact gggttttagg attgaatacc aacgtccaga agaaaacttt ctctttctct
    11401 tgaagagtgt caactaatgt tagaggaggg aacaaaggat caactagctg aactgaccta
    11461 tccatttggt cgtggagtca atctctcctt tggcataaag gatgtttcaa aactctatta
    11521 aagagtgatg aaggcaaact atcctattta tcgtcccttg accaagagaa agtttcgtgt
    11581 cagtgatccc tatatctatc ctcatgaatt tgcagttttg gatccaaatg gctatttttt
    11641 aagatttagc gagtagaata ataaaaattg ggaaaagtct ttaaagttag aaaaaggcat
    11701 attgtcaggt gtctaaaaac ttgatagtat gccttttatg atggaaaaat cttctaacac
    11761 tcttcgaaaa tctcttcaag ccacatcaac gtcgccttac cgtaggtata tgatactgac
    11821 tctgtcaatt ctatccacaa cctcaaaaca gtgatttgag gttgtggata gcttcctagt
    11881 ttgctctttg attttcattt agtataaatg ttagcttaca acggagtttt tgttcacccc
    11941 ctcttctgtt tgttcgtttt ttaaaccaaa gcactaacaa aatagttgat ttataaagtt
    12001 atttagtgta aaataaaaac attggctcaa gcctatttaa aattgaatat cgttttactt
    12061 gtttatgtcg tgaattggca cgacgtttct acaaggtgcc ggaacaccta acaataagta
    12121 agtcagcagt gagatatggc cgtttttgcc atgcctattt gggggactta cttagagatt
    12181 aagtaggtct tttttgaata tttttaatat ttcccttaat tagtgcggac gggaggtgaa
    12241 attatcctgt gaataatttc aggaatccag ggaatttcat ccctaatttt tgagccccta
    12301 gctctttcat ttctaggctt aggctaaaat agttccccga actattttac tctcaaaaat
    12361 cccgtcatag acaataacaa gattattgtc tatgatacca ctacaacggg aaatatttga
    12421 taagactcgc ttcgctcgta atagttttat caaatatata gtattagcaa aagggatcac
    12481 tatagaaaca tttaaacaac aagtaaatta acttttagac tttaggaggt cttatgaaat
    12541 tattagaaga gcgcatcctc aaggatgggc atatcttggg tgataacatc ctcaaggtag
    12601 attccttttt aacccaccaa gttgacttta gcttgatgcg agagattggt aaggtttttg
    12661 cggaaaaatt tgctactact ggcattacca aggtcgtaac cattgaagcg tcgggtattg
    12721 ccccagccgt ttttacagct gaagccttaa acgttcccat gattttcgcc aaaaaagcta
    12781 agaacatcac catgaacgaa ggcatcttaa ctgctcaagt ctactccttt accaagcagg
    12841 tgaccagcac cgtttctatc gctggaaaat tcctctcacc agaggacaag gttttgatta
    12901 tcgacgattt ccttgctaat ggccaagctg ctaaaggctt gattaaaatc atcgaacagg
    12961 ccggtgccac agtccaagct atcggtatcg tgattgagaa atccttccaa gatggtcgtg
    13021 atttgcttga aaaagcaggc taccctgtcc tatcacttgc tcgtttggat cgttttgaaa
    13081 atggtcaggt cgtatttaag gaggcagatc tctaatgcaa actcaagaaa aacactcgca
    13141 agcagccgtt cttggcttgc agcacttact agccatgtac tcaggatcta tcctggttcc
    13201 catcatgatt gcgacagccc ttggctattc agctgagcag ttgacctacc tgatttctac
    13261 agatatcttc atgtgtgggg tggcaacctt cctccaactc caactcaaca aatactttgg
    13321 gattggactc ccagtcgttc ttggagttgc attccagtcg gtcgctccct tgattatgat
    13381 tgggcaaagc catggtagtg gcgctatgtt tggtgccctt atcgcatctg ggatttacgt
    13441 ggttcttgtt tcaggcatct tctcaaaagt agccaatctc ttcccatcta tcgtaacagg
    13501 atctgttatt accacgattg gtttaacctt gatccctgtc gctattggaa atatgggaaa
    13561 taacgttcca gagccaactg gtcaaagtct cttgcttgca gctattactg ttctgattat
    13621 cctcttgatc aacatcttta ccaaaggatt tatcaagtct atctctattt tgattggtct
    13681 ggttgttgga actgccattg ctgctactat gggcttggtg gacttctctc ctgttgcggt
    13741 agctccactt gtccatgtcc caactccact ctactttggg atgccaacct ttgaaatctc
    13801 atctattgcc atgatgtgta tcatcgcaac ggtgtctatg gttgagtcaa ctggtgttta
    13861 tctagccttg tctgatatca caaaagatcc aatcgacagc acgcgccttc gcaacggtta
    13921 ccgcgcagaa ggtttggcgg tacttctcgg agggatcttt aacaccttcc cttacaccgg
    13981 attttcacaa aacgttggtt tggttaaatt gtcaggcatc aaaaaacgcc tgccaatcta
    14041 ctacgcagct ggtttcctgg ttctccttgg actgcttcct aagtttggtg cccttgccca
    14101 aatcattcca agctccgtcc tcggcggtgc catgctggtg atgtttggtt ttgtatctat
    14161 tcaagggatg caaatcctcg cccgagttga ctttgctaac aatgaacaca acttccttat
    14221 cgcagctgtt tcaatcgctg caggtgtcgg tctcaacaac agtaatctct ttgtcagcat
    14281 gccgacagcc ttccaaatgt tcttctcaaa cggaatcttc gtagccagcc tactcgccat
    14341 tgtcctcaat gccgtattaa atcataaaaa gaaataaaaa aaagaggtgc gagcctcttt
    14401 tttgtttatc acaaattgat tataaataca atcaatccca attcccatct aattgaattg
    14461 gacctatttc aaattct
//
LOCUS       DAVLVZ010000045        14079 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000045 DAVLVZ010000000
VERSION     DAVLVZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.045"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            37..939
                     /locus_tag="VYN77_001439"
                     /pseudo
     CDS             37..939
                     /locus_tag="VYN77_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1003..2085)
                     /locus_tag="VYN77_001440"
     CDS             complement(1003..2085)
                     /locus_tag="VYN77_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1558885.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(2082..3059)
                     /locus_tag="VYN77_001441"
     CDS             complement(2082..3059)
                     /locus_tag="VYN77_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1558886.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPVVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            3274..3651
                     /locus_tag="VYN77_001442"
     CDS             3274..3651
                     /locus_tag="VYN77_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1558887.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            3886..5277
                     /locus_tag="VYN77_001443"
     CDS             3886..5277
                     /locus_tag="VYN77_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1558888.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            5391..6233
                     /locus_tag="VYN77_001444"
     CDS             5391..6233
                     /locus_tag="VYN77_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1558889.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            6506..7267
                     /locus_tag="VYN77_001445"
     CDS             6506..7267
                     /locus_tag="VYN77_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1558890.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            7460..8377
                     /gene="glyQ"
                     /locus_tag="VYN77_001446"
     CDS             7460..8377
                     /gene="glyQ"
                     /locus_tag="VYN77_001446"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1558891.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            8589..10625
                     /locus_tag="VYN77_001447"
     CDS             8589..10625
                     /locus_tag="VYN77_001447"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1558892.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            10667..10924
                     /locus_tag="VYN77_001448"
     CDS             10667..10924
                     /locus_tag="VYN77_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1558893.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(11147..12391)
                     /locus_tag="VYN77_001449"
     CDS             complement(11147..12391)
                     /locus_tag="VYN77_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1558894.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(12410..13015)
                     /locus_tag="VYN77_001450"
     CDS             complement(12410..13015)
                     /locus_tag="VYN77_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1558895.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            complement(13075..13998)
                     /locus_tag="VYN77_001451"
     CDS             complement(13075..13998)
                     /locus_tag="VYN77_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1558896.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
BASE COUNT         4274 a         2573 c         3093 g         4139 t
ORIGIN      
        1 agcagccaag gtgtactgtt atagattcaa tacactatag actgtaatca aacaacgatt
       61 tggcgaaatg taaaaaaata tgaggagttc ggactcgact ctctccttca agaaacacgt
      121 ggtggtcgta accatgcata tatgacggtt ggggaagaga aagcctttct tgcccgtcat
      181 ttgaaggctg cagaggcagg agaatttgtt acaattgatg ccttatttca ggcttataaa
      241 aaggagttag gtcgttccta cacacgtgat gccttctatc aactgttgaa gcgccatggt
      301 tggcgaaata ttatgccacg tccagaacat cctaagaaag cagacgctca aaccattgtc
      361 gcgtctaaaa ataaaatctc aattcaagaa gaaaagaaag cgttttaaaa ccagtagacc
      421 ttttcataag gttcgcttga tgtaccaaga tgaggctggc ttcggtagaa tcagtaaact
      481 gggatcttgt tggtctccaa taggagtagg tccacatgtc catagtcact atatacgaga
      541 atttcgctat tgttatggac ccatacaggc gaatcatttt tcttaatagc tggtggatgt
      601 aatactgagt ggatgaacgt ctttttagaa gagctttcac aagcttatcc agatgattat
      661 cttttattcg ttatggacaa tgctatatgg cataaatcaa gtaccttaaa aattccgact
      721 aatattggct ttgcatttat tcctccatac acaccagaga taaaccccgt tgaacaagtg
      781 tggaaagaga ttcgtaaacg tggatttaag aataaagcct ttcgaacttt ggaagatgtc
      841 atgaatcaac ttcaagatat tatacaagaa ctggagaagg aggtgataaa gtccatcgtt
      901 aatcggagat ggattagaat gctttttgaa aacagatgag tataaaattg aattgcttgt
      961 aaaaaagctc catacactgg atgtgtatag agcaatgggg ctttatttga tatagagttc
     1021 ttggtttttt aggacaattt ctcggatact tgcaaacttt ttaagttttt tgatttcttc
     1081 tggatgagtg acgagagtaa taacatatcc ttccttgccc atacggccag tacggccagc
     1141 acggtgtgtg taggtttcgc tatctctagg aatatcaaag tttacgacac attctaggct
     1201 atcgatatca attccacgag ccaaaaggtc agttgcaaga agcagggtta gttggttatc
     1261 tttaaacttt tctaagatga cttttctaaa tttaacatta acatcactag cgagggaaac
     1321 agccaatata tcacgatact gtagtttttc ctcggcattc ccaaggtctg acaggctatt
     1381 gaagaagact agaccacgga aatcctctac atgagccagt tttcgtagta tatccactcg
     1441 atgacgttgg tctacctgca tgtagaaatg ctggatattg tccaattttt gatcagagag
     1501 atcaatagtg cgtgtattcg gtacaatctt ttcttgatca aacttggtcg tggcactcat
     1561 gtagaccagt tggtggtcac gaggtgcgta gtgagtgatt ttttctacaa agtgaatctg
     1621 agaatcatct agtaattggt caaattcatc caggatgatg gtttccacat tcatcatctt
     1681 gattttttta agtttaatga gttcaaagat acggccagga gttccaatca gaatttctgg
     1741 ccccttttta agacgttcaa tttgtcgttt ctgacttgaa cctgaaagga agagttgagc
     1801 agtcaatccg atagcttctg cccacgtttt acatacatca aaaatctgtc cagcaagttc
     1861 cgtatttggt gctagaatca agagttgttg ggcttttttc ttttgtagtc tgagaagact
     1921 tggtaggaga taagctaggg tcttaccagt tccggtttgg ctcactccta ggaggttttc
     1981 tccagcaaga aggggctcaa atagttgagt ttgaatgggg gtgaattctt ggaaaccgag
     2041 ttggtcactc agttcttgcc attcagtcgg tagtttggtt ttcatttttc tgcctcaaat
     2101 ctaatgccag cagtctggcg catggtatat agtagctcat gaacagagcc tgcatcatac
     2161 agccaagttt ggtagagatt cagatctggt tgctggatca tgtgtgcaaa tgcagcgact
     2221 tcctcagtca tcgtatgagg agcctgttgg ataggaagct ggacttgatt tccttggtgg
     2281 tcggtaaaaa tagctgagcg aatatgctca atcgtgttga gagtcaaggt tccatctgtt
     2341 gtataaatct cgcaaggaag attggaagtg atgttttttc cagccttgat gtgaacttga
     2401 tagtctgggt agaagaggat accatctcca tttaggtcaa tgctattgtc aagctgttga
     2461 gcatggtaag tcgcgtcatt agctttttca aaaagacgaa cagcagcata gaggggataa
     2521 atccccaaat ccatgagggc tccaccagca aaacggtctg aaaagacatt tggtgtttgt
     2581 ccagccaaca agtcaggcat cttggaagag tatttggcat agttgaaatc tgctcctaac
     2641 acttgcttat ctgctaaaaa gtttttgata gtagtaaagg ctttctcgtg gtaattacga
     2701 gctgcttcaa agataaaaca gttatttttt tcagctgttt gaatcaaatc aaaccattct
     2761 tgtggttgag tgacaactgg cttttcgaga ataacatgtt taccagcaga caaggcagct
     2821 tttgcctgag caaaatgtaa ggagtttgga ctggcgatat agactaaatc aaaagaagat
     2881 ttgaagaaga cttctaattg atcgaatagt tggatattct gatagcgaga agcaaaggtt
     2941 gctgcagttt ctagttttct agaatagatt gcgaccagtt ggtattctcc actggtatgg
     3001 gctgcttcta tgaaatgatg gctgatagcg ccagttccga tgacacctaa ttttagcata
     3061 aatactcctt ttccgatttt aaatccttct ttcattataa catagataga cgggactatc
     3121 caacagagag gagaaaattt caaataagct attagctttc ttttccgaat aaatagatag
     3181 aagcatagaa tctagcaaac ctagatttaa aaatgtgcta taatagaagg aggaaaagga
     3241 ggattctcag acatccaggt atcagcccaa ctaatgattt gtcaatttat ccgcgatatg
     3301 ctggacttgc cagcaaaaaa tgtgacgatt ttggagggaa gtaacattca cgtcttgcct
     3361 tccatgccct actcagcgca agatttctat actagtatag acgtcttggc ggagttagat
     3421 aatggaatcc aagttatcat cgaaattcag gttcatcatc agaatttttt catcaatcgc
     3481 ctatgggctt atctgtgcag tcaggttaat caaaacctag aaaaaattcg ccaacgtgaa
     3541 ggtgataccc accagagcta caaacaaatc gcaccagtat acgctatcgc aattgtcgat
     3601 agtaattact tctcagatga cctagctttt catagtttta tagtaaaatg aaatgagaac
     3661 aggacaaatc gatcaggaca atcaaatcga tttctaacaa tgttttagaa gtataggtct
     3721 actattctag cttcaatcta ctagaaattc catagataga aaactacata atctctacag
     3781 atacggatgt tggagttgat gtaagatgct ttggcttgct agaggaattg tggattgcca
     3841 aattgtatca ttgaaattat tgctcaaatt tgttatgata taaatatgaa taaaagtaga
     3901 ctaggacgtg gcagacacgg gaaaacgaga catgtattat tggctttgat tggtatttta
     3961 gcaatttcta tttgcctatt aggcggattt attgctttta agatctacca gcaaaaaagt
     4021 tttgagcaaa agattgaatc gctcaaaaaa gagaaagatg atcaattgag tgagggaaat
     4081 cagaaggagc attttcgtca ggggcaagcc gaagtgattg cctattatcc tctccaaggg
     4141 gagaaagtga tttcctctgt tagggagttg ataaatcaag atgttaagga caagctagaa
     4201 agtaaggaca atcttgtttt ctactataca gagcaagaag agtcaggttt aaagggagtc
     4261 gttaatcgta atgtgaccaa acaaatctat gatttagttg cttttaaggt tgaagagact
     4321 gaaaagacca gtctaggaaa ggttcactta acagaagatg gccaaccttt tacacttgac
     4381 caactgtttt cagatgctag taaggctaag gaacagctga taaaagagtt gacctccttc
     4441 atagaggata aaaaaataga gcaagaccag agtgagcaga ttgtaaaaaa cttctctgac
     4501 caagacttgt ctgcatggaa ttttgattac aaggatagtc agattatcct ttatccaagt
     4561 cctgtggttg aaaatttaga agagatagcc ttgccagtat ctgctttctt tgatgttatc
     4621 caatcttcgt acttactcga aaaagatgcg gccttgtacc aatcttactt tgataagaaa
     4681 tatcaaaaag ttgtcgctct aacctttgat gatggtccaa atccagcaac gaccccgcag
     4741 gtattagaga ccctagctaa atatgatatt aaagcgactt tctttgtgct tgggaaaaat
     4801 gtttctggga atgaggactt ggtgaagagg ataaaatctg aaggtcatgt tgttggaaac
     4861 catagttgga gccatccgat tctctcgcaa ctctctcttg atgaagctaa aaagcagatt
     4921 actgatactg aggatgtgct aactaaagtg ctgggttcta gttctaaact catgcgtcca
     4981 ccttatggtg ctattacaga tgatattcgc aatagcttgg atttgagctt tatcatgtgg
     5041 gatgtggata gtctggactg gaagagtaaa aatgaagcat ctattttgac agaaattcag
     5101 catcaagtag ctaacggctc tatcgttttg atgcatgata ttcacagtcc gacagtcaat
     5161 gccttgccaa gggtcattga gtatttgaaa aatcaaggtt atacctttgt gaccatacca
     5221 gagatgctca atactcgcct aaaagctcat gagctgtact atagtcgtga tgaataagca
     5281 agaaaaaata ggtctgttag atatttgaca gacttatttt ttacagaaga tagtactact
     5341 taaaaaatgt tttatgctat aatcgatgaa taaaatagaa ggagaagcat atgaatacct
     5401 atcaattaaa taatggagta gaaattccag tattgggatt tggaactttt aaggctaagg
     5461 atggagaaga agcctatcgt gcagtgttag aagccttgaa ggctggttat cgtcatattg
     5521 atacggcggc gatttatcag aatgaagaaa gtgttggtca agcaatcaaa gatagcggag
     5581 ttccacgtga agaaatgttc gtaactacca agctttggaa tagtcagcaa acctatgagc
     5641 aaactcgtca agctttggaa aaatctatag aaaaactggg cttggattat ttggatttgt
     5701 atttgattca ttggccgaac ccaaaaccgc tcagagaaaa tgacgcatgg aaaactcgca
     5761 atgcggaagt ttggagagcg atggaagacc tctatcaaga agggaaaatc cgtgctatcg
     5821 gcgttagcaa ttttcttccc catcatttgg atgccttgct tgaaactgca actatcgttc
     5881 ctgcggtcaa tcaagttcgc ttggcgccag gtgtgtatca agatcaagtc gtagcttact
     5941 gtcgtgaaaa gggaatttta ttggaagctt gggggccttt tggtcaggga gaactatttg
     6001 atagcaagca agtgcaagaa atcgcagcaa atcacggaaa atcgattgct cagatagcct
     6061 tggcctggag cttggcagaa ggatttttac cacttccaaa atctgtcaca acctctcgta
     6121 ttcaagctaa tcttgattgc tttggaattg aactgagtca tgaggagaga gaaaacttaa
     6181 aaacgattgc tgttcaatcg ggtgctccac gagttgatga tgtggatttc tagaaaatca
     6241 taaaaagaat tgtacattat tctaattttt gatataatag tcagcaggaa agaaagtctt
     6301 atggcgttct tcaagcgagc ttgggatagt gggagccaag tagggcaaaa taatgagctg
     6361 gcgctttctg tagtattttc aaaaacaatg aagtaataaa ttagggtgga accgcgtttc
     6421 tgacgcccct aggttaaatc aacctaggat tgtcggacgc ggttcttttg cttattcagt
     6481 ctattgtgtg aaagaaagga gagccgtgga caacctttat cttgtaaaag acgatagtca
     6541 actagctaca tttcgtgatt ttgtagtaag aaatactgaa aagttgaaag attatcaatc
     6601 ttttttaaag aatgaacttg cagtctgtga tttaccgcaa gccgttattt ggtcagattt
     6661 taatgctgct acacagatta ttagggaaag cgctgttcca gcctatacaa ataatagacg
     6721 aatggttatg gcgcctgatt tagctgtttg gaaagagttg tatttgtatc aattgatgga
     6781 ctacgagtgt tctcagcaaa ctcaagcaat agaaagtcac tatcattctt tatctgaaaa
     6841 tttcctctta cagattgtag gacatgagtt agctcattgg tcggagcatt ttttagatga
     6901 ttttgatggt tatgactctt atatctggtt cgaagagggg atggttgaat atattagtcg
     6961 caagtatttc ttgacagaag aggaatttca agcggaaaaa atttgtaatc aatctctcgt
     7021 agaacttttt cagaagaagt atggttggca ttcattgaat gattttggtt cttcgactta
     7081 tgataagaac tatgcaagta ttttttatga atactggcgc agctttttga cagtagataa
     7141 gttggtagaa aatttaggta gtgtacaagc ggtcttagat tcttatcatt tatgggcaaa
     7201 tacagaaaaa acttttccct tgttagattg gtttgttcag cagaaattaa ttgaaaaaga
     7261 aatataaaaa ctaaaggagt aaacaatgtc taagaaattg acatttcact gcgtcagtgg
     7321 cagaaacctc cttacagtcg gactgcccca cgctcagcac tagagtgcct gagctagacg
     7381 cagtactaac tcgtcttgcc tcgtatgatc gacgaggcag actcatgtcg caagtaattt
     7441 atataaaaag gagtaaacaa tgtctaagaa attgactttc caggaaatca tccttacttt
     7501 gcaacaattt tggaatgacc aaggttgtat gcttatgcag gcttatgata atgaaaaagg
     7561 tgcgggaaca atgagtcctt acactttcct tcgtgctatc ggacctgagc catggaatgc
     7621 agcttatgta gagccatcac gtcgtcctgc tgacggtcgt tatggggaaa accctaaccg
     7681 tctctaccaa caccaccaat tccaggtggt catgaagcct tctccatcaa atatccaaga
     7741 actttacctt gaatctttgg aaaaattggg aatcaatcct ttggagcacg atattcgttt
     7801 tgttgaggac aactgggaaa acccatcaac tggttcagct ggtcttggtt gggaagtttg
     7861 gcttgacgga atggaaatca ctcagttcac ttatttccaa caagtcggtg gattggcaac
     7921 tggccctgtg actgcggaag ttacctatgg tttggagcgc ttggcttctt acattcaaga
     7981 agtagactct gtctatgata tcgagtgggc tgatggtgta aaatacggag aaatctttat
     8041 ccagcctgag tatgagcact caaaatattc atttgaaatt tcggaccaag aaatgttgct
     8101 tgaaaacttt gataagtttg aaaaagaagc tggtcgtgca ttagaagaag gcttggtaca
     8161 ccctgcctat gactatgttc tcaaatgttc acataccttt aatctgcttg acgcgcgtgg
     8221 tgccgtatct gtaacagagc gtgcaggcta tatcgctcgt atccgtaact tggcccgtgt
     8281 cgtagccaaa acctttgtcg cagaacgcaa acgcctaggc tacccacttt tggatgaaga
     8341 aacaagagct aaactcctag cagaagacgc agaataaaga gagtgacaaa ttacgaaaat
     8401 gggcgaacag agtgagccct gagccagttg ccgcagtgat gaaggtatcc ttagtgaaac
     8461 taaggatact aggcaaaatt ggagactttt ggctccaatt ttagcaatga aacaacgaag
     8521 ttggttgctt gcgtgccaat cacataaggc aaactggaaa ataaaaagat acttttcgga
     8581 gaaaaaacat gacaaaaaac ttattagtag aactcggtct tgaagaatta ccagcctatg
     8641 ttgtcacacc aagtgaaaaa caactaggcg aaaaaatggc agccttcctc aaggaaaacc
     8701 gcctgtcttt tgaagccatt caaaccttct caacaccacg tcgtttggct gttcgtgtga
     8761 ctggtttatc agacaaacag tctgatttga cagaagattt caagggacca gcaaagaaaa
     8821 ttgccttgga tagtgatgga aacttcacca aagcagctca aggatttgtc cgtgggaaag
     8881 gtttgactgt tgaagatatc gaattccgtg aaattaaggg tgaagaatat gtctatgtca
     8941 ctaaggaaga agttggccaa tcagttgaag ccattgttcc tggtgttgta gatgtcttga
     9001 agtcattgac tttccctgtc agcatgcact gggctggaaa tagctttgaa tacatccgcc
     9061 ctgttcacac tttaactgtt ctcttggatg agcaagagtt tgacttggat ttccttgata
     9121 tcaagggaag tcgtgtgagc cgtggccatc gtttcttggg gaaagaaacc aaaattcagt
     9181 cagcattgag ctatgaagaa gaccttcgta agcagtttgt aatcgcagat ccatgtgaac
     9241 gtgagcaaat gattgttgac caaatcaagg aaattgaggc aaaacatggt gtacgtatcg
     9301 aaattgatgc ggatttgctg aatgaagtct tgaatttggt tgaataccca actgccttca
     9361 tgggaagttt tgatgctaaa taccttgaag ttccagaaga agtcttggtg acttctatga
     9421 aggaacacca gcgttacttt gttgttcgtg atcaagatgg aaaactcttg ccaaacttca
     9481 tttctgttcg taacggaaac gcagagcgtt tgaaaaatgt catcaaagga aatgaaaaag
     9541 tcttagtagc ccgcttggaa gacggagaat tcttctggcg tgaagaccaa aaattggtga
     9601 tttcagatct tgttgaaaaa ttaaacaatg tcaccttcca tgagaagatt ggttcccttc
     9661 gtgaacacat gattcgtacg ggtcaaatca ctgtactttt ggcagaaaaa gctggtttgt
     9721 cagtggatga aacagttgac cttgctcgtg cagcagccat ttacaagttt gacttgttga
     9781 caggtatggt tggtgaattt gacgaactcc aaggaattat gggtgaaaaa tacacccttc
     9841 ttgctggtga aactccagcg gtggcagctg ctattcgtga acactacatg cctacatcag
     9901 ctgaaggaga acttccagag agcaaggtcg gcgcagttct agccattgca gacaaattgg
     9961 atacgatttt gagtttcttc tcagtaggat tgattccatc aggttctaat gacccttatg
    10021 cccttcgtcg tgcaactcaa ggtgtggttc gtatcttgga tgcctttggt tggcacattg
    10081 ctatggatga gctgattgat agcctttatg cattgaaatt tgacagtttg acttatgaaa
    10141 ataaagcaga ggttatggac tttatcaagg ctcgtgttga taagatgatg ggctctactc
    10201 caaaagatat caaggaagca gttcttgcag gttcaaactt tgttgtggca gatatgttgg
    10261 aagcagcaag tgctctcgta gaagtaagca aggaagaaga ttttaaacca tctgttgaat
    10321 cactttctcg tgcctttaac ctggctgaga aggcagaagg ggttgctaca gttgattcag
    10381 cactatttga gaatgaccaa gaaaaagctt tggcagaagc agtagaaaca ctcgttttat
    10441 caggacctgc aagtcagcaa ttgaaacaac tctttgcgct tagcccagtc attgatgctt
    10501 tctttgaaaa tactatggta atggctgaag atcaggctgt ccgtcaaaat cgtttggcaa
    10561 tcttgtcaca actaaccaag aaagcagcta agtttgcttg ttttaaccaa attaacacta
    10621 aataaaattt gataaacgga ctttatctta ttacaaagga gaagaaatgg atccgaaaaa
    10681 aattgctcgt atcaatgagc ttgctaaaaa gaaaaaaaca gaaggcttaa caccagaaga
    10741 aaaagtggaa caagccaaac tacgtgagga gtacatcgaa ggttatcgcc gcgctgttcg
    10801 tcaccacatt gaaggaatca aaattgtgga cgaagaagga aacgatgtta caccagaaaa
    10861 actacgccaa gtacaacgtg aaaaaggatt acatggccgt agtcttgatg atccaaattc
    10921 ataataatac tcttcgaaaa tcaaattcaa accacgtcag cttcaccttg ccgtacttaa
    10981 gtacagcctg cggctagctt cctagtttgc tctttgattt tcattgagta tatgtattct
    11041 ttcttttaac aaagatagat gaaacgataa caaagagact agcagtttgt gtttgctagt
    11101 cttttttcgc taaaaaagga accataatgg ttcctaaaaa ctatcattag taacttgcac
    11161 cggctgtagc gtctgcgtca ccaccgtggc ctccagcatc ccctgaatca gaagcgccag
    11221 aagtagcatc ggcgtctcca tgacctccgg cagcaggagc aaatggtccg ctaccaccca
    11281 ccaaacgttg accagtctct tttaggtacc agtcaagcca tggttggaag ttaaagacga
    11341 tttcattgat accagcgtat gatccatcag gatagtacat tgcttggtag ttgtgagtgt
    11401 tgataacacc tgcaggagaa cctggaacga tcgtacggac gtattcttgg tttccgttgc
    11461 gaagtgttcc gataacccac tctacgttct tcatacgtgc tggtggaaga gaaccatgaa
    11521 cagtcgacat acggctacct gattgaggtg gtacacgttt agcgaacata gtgtctggat
    11581 cttggtgagc gttgttgtag tagaggaatt ggttgttgtc gtcagcgtat gtcaattcaa
    11641 atggcatagc tttcaagaac atatcaattt ggttaactgt taggataccg tggtccaatt
    11701 tgacataggt atcaccagaa acagcaccag tgaatgctgc aactttttct acccattctg
    11761 gatcgtcagg gtcaacttct gtgatggttg tagcgattgg ttttccacaa tccaagtctt
    11821 ctgattcgat tggttttggt tttttcaatt tcgaaacgac tcctacgtat ttaacaaagt
    11881 tatctaagca agtttcaagg aatttaacag tgccttcgtt ggtgatattt ccgttgttat
    11941 caaaagcttc cttagcttta ccaagaagga attcgttacc tggaagcgtg taggcattaa
    12001 cacctggagc atcaaggatt ttacgaaggt gaacttgagc acgtgatgtt ccttggtcat
    12061 agtatgatgc acccacaatc ataacaggct tgttttcaaa tggatgaact tcgtatgaaa
    12121 gccattcaag tacagatttg agtgaagctg agatagtgtg gttatgctca ggagtagcaa
    12181 tgataacacc atctgcacga gtaattttgt tatataaata acgtaattgg aaactttcat
    12241 cccatttttc atcttggtta aacattggaa cttcgtcaat ttcaagaact tctaattcaa
    12301 atttgagttt gaagtagcga cggataaatt ccaagagctt acggttatat gattgatcgt
    12361 agtttgatcc aacaagtcca acaaatttca ttctttttgg tctcctatct tacaaatttt
    12421 cccagtcaaa gtcttcagca tctttgcgaa gtaattcttg tgcattacgt aatttttctg
    12481 tgatttttac aaagatacgg aagtcatcaa agatggcatc caatttcttg ataacatcaa
    12541 ggtcaaccaa gtcgccactt gggttaaatg cttgaagaga gtgtgagagc aagaattcat
    12601 ctggaagaac atttgccttg atttcaggag cattcaagat ttgacgaagt tgcaattggg
    12661 cacgagatga accaagcgta ccgtaagaag cacctgtaat catgattggt ttgttcaaaa
    12721 gtgggtaaat accataagac aaccaagcaa gagcgctcat caaaacagct ggaatagagt
    12781 gatcatactc aggagtaccg ataataacgc catctgcctc ttcgatttta gcagcaattt
    12841 ccaatatttc agcaggtact tgcttgtcag ctggtttgtt gaagacagga atggccttga
    12901 tttcaacaag ttcaatttca gctttgtcag taaagtgttt ttgcatgtat tgaagcaatt
    12961 gacggtttgt agaacgtttt gaatttgttc caacaatagc aataagtttt aacatgagat
    13021 ttcctttctc tttttacata atacaatttt aaaattccat tgaaacagtt gtctctatag
    13081 agtaggaatt cctgaagaac agcttaggtg gccttcttta tcgatgagga tgacttcgat
    13141 gccctccaaa ctttcgactt gccagaggat agaagcaggt ctttctccaa agagtcgagt
    13201 cgtccagatt tcgccatcga ctgatttatc agagatgatt gttagactcg ctagttccgt
    13261 ttcaacagga tatcctgttt gactgtcaaa aatgtgatgg taatcttgtc catcgacggt
    13321 caggtgacgt tcataaatgc ctgaagtcac gacagattta ttgacaacag ggatggtcat
    13381 taaatgattt cccctaggat tggctgggtc ttgaatcccg atttgccatg ggttatcccc
    13441 tcttgcctga ttttttccaa tggtcaggat attccctccc agattgatca aggcagaagt
    13501 caccccctct ttcctaagaa attgggcaac cttatccgca ctgtatcctt tggctaaaca
    13561 acctagatcg atcttcattc ctttctgttt taaaaacaca gtagaagtag aagaatctaa
    13621 ctcgatacca tgaggattga ttagaggcag caccgattca atttcttgag gctgggcgac
    13681 cttggcatct gaaaaaccga tacgccaggt ttgaattaag ggaccaatgc tgatattgag
    13741 gtggctagag agcgctaggc tatgctctaa cccaagtgaa atcagctcaa acaggtctgg
    13801 atgaaccgtg acgggggcta ttcctgcttg ataattgatt tccatcaact cagattcttg
    13861 actattggcg ttgaagcggt attcaagttc tttgagcaag tcaaaggatt tttggagaaa
    13921 gatatcggct tgctcatcca ctaatgaaat agtgatagta gtccccatta gccgttcaga
    13981 atgtgaacga agagtcaagc taccaactcc tttctcttat agaaaataag ttgtaatatc
    14041 aaataatcat ctaaattgaa gcccttacat ttcattttc
//
LOCUS       DAVLVZ010000046        14048 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000046 DAVLVZ010000000
VERSION     DAVLVZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.046"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            83..880
                     /locus_tag="VYN77_001452"
     CDS             83..880
                     /locus_tag="VYN77_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1558897.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..1150
                     /locus_tag="VYN77_001453"
     CDS             1028..1150
                     /locus_tag="VYN77_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEV1558898.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1172..2212
                     /locus_tag="VYN77_001454"
     CDS             1172..2212
                     /locus_tag="VYN77_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEV1558899.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFFDIDNLEEVEAAIKDNTKLVLIETLG
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2383..3657
                     /locus_tag="VYN77_001455"
     CDS             2383..3657
                     /locus_tag="VYN77_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1558900.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VYN77_001456"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VYN77_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1558901.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4786..5088
                     /locus_tag="VYN77_001457"
     CDS             4786..5088
                     /locus_tag="VYN77_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1558902.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5196..5834
                     /gene="udk"
                     /locus_tag="VYN77_001458"
     CDS             5196..5834
                     /gene="udk"
                     /locus_tag="VYN77_001458"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1558903.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5962..7302
                     /locus_tag="VYN77_001459"
     CDS             5962..7302
                     /locus_tag="VYN77_001459"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1558904.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEEAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7280..7492
                     /locus_tag="VYN77_001460"
     CDS             7280..7492
                     /locus_tag="VYN77_001460"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1558905.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7489..8364
                     /locus_tag="VYN77_001461"
     CDS             7489..8364
                     /locus_tag="VYN77_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1558906.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQVPFE
                     TESIVSVVESLRING"
     gene            8357..9172
                     /locus_tag="VYN77_001462"
     CDS             8357..9172
                     /locus_tag="VYN77_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1558907.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9165..9596
                     /locus_tag="VYN77_001463"
     CDS             9165..9596
                     /locus_tag="VYN77_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1558908.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9603..11270
                     /gene="recN"
                     /locus_tag="VYN77_001464"
     CDS             9603..11270
                     /gene="recN"
                     /locus_tag="VYN77_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1558909.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11272..12000
                     /locus_tag="VYN77_001465"
     CDS             11272..12000
                     /locus_tag="VYN77_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1558910.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFIDQGMTEHHFIENDGVVAED"
     gene            12079..13902
                     /gene="lepA"
                     /locus_tag="VYN77_001466"
     CDS             12079..13902
                     /gene="lepA"
                     /locus_tag="VYN77_001466"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1558911.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         4151 a         2560 c         3356 g         3981 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcggaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt tttgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat aatatggagg
     1021 tcaccttatg acttgtgatt ttaaatttga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atactatttt ggcgattgcc catgctggtg accatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttt tcgatattga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac cccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaaca ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgtgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggacggctt cagggaaatt ccctcaattt gttgacgagg gtccaagctg
     1741 ccacaatttg agctatactc gtgatgtggg tgcagcagcc tttattatag ctgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa acctcttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttatcat gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtggcgagg aagaagcacg caaggtcatt gataatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgctaaatc gcttgttgtc catccagcaa caaccactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaact aaattcgttt
     2221 gtcaatcggt cttgaaaatg tagaagattt gattgaagac ttgcgcttgg ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg cttcgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaaa gaagtctaga aagaggaacg atatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gagagtcagt atgccgaact cttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaagcagga actggccttg
     2521 gctgagcaaa aggccatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattac agagtcagat ccaaaacttt gatacagaaa aagaattggc caagaaagag
     2641 gttgaacaga caagccatga ggctctcttg gctaaggaca aggaagtaca gctcttagaa
     2701 aatcagttgg ctaccttgcg tttggagcat gaaaatcaac tacaaaagac cctttctgac
     2761 ctagaaaaag aacgggatca ggttaaaaac caactacttt tgcaggaaaa ggaaaatgaa
     2821 ttatctttgg cttctgttaa gcaaaactac gaagcccagc tcaaggcagc tagtgaacaa
     2881 gtcgagtttt ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggctgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagtcacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc aattgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgacc
     3541 acatctgaaa accaactccg tttagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat taacttaaaa aaggaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaatcactc tgggctattc cacgaagact
     3901 gaggatgcta gtggggaagt ggtcgcagaa acccctgttt tgtctctctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta ctcagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac gtacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gacgctcttg ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataaca attgcttgat aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct atatagaaat
     4681 ttagaggtgt gaaatgaagc aatttaaaat tctttcagat aaatatttag agtccattac
     4741 aggttctgat gggaacttag gcccaggatt tggtgtgata attccatgat gcgaaatgag
     4801 tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg
     4861 agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaata ccaatcggtc
     4921 atggccaatt tgagtcaagg gttgtcactt tacctcatga cgcatcatta ccaggcacct
     4981 aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga aagagggaga
     5041 ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca
     5101 tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta aaaaatgtga
     5161 tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca atcattattg
     5221 gagtgacagg tggttctggt ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt
     5281 ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat cagtctcatt
     5341 tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt gatacagact
     5401 tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc ccgacctacg
     5461 actatacaga gcatacacgg agtagcaaga cctatcgtca ggaacctcaa gatgtcttta
     5521 tcgttgaggg tattttggtc ttggaggaca agcgtctgcg cgatttgatg gatatcaaga
     5581 tttttgtgga tacggatgac gatgtgcgca ttattcgtcg tatcaagcgt gatatggagg
     5641 agcgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc aaaccaatgt
     5701 accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct gaaggggtta
     5761 gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg gaagaagctc
     5821 gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt ccctcataat
     5881 ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat gaaagagcaa
     5941 gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt gaccaagtat
     6001 ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac tggtcaagtt
     6061 tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga ccatgcagtt
     6121 attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct ggaagaagga
     6181 atgaagatca atgtgattgg gcgtgtacag gtctatgaac caagtggtag ctactccatc
     6241 atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt tgaacaactt
     6301 aagaaaaaat tgacagaaga aggcctgttt caagaacgct tcaagcaagc tctaccccaa
     6361 ttttctaaga gaattggggt agtaaccagc cgtagtggag ccgttattcg agatattatc
     6421 acgaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatccgac caaggttcaa
     6481 ggtgaagagg ctgcggagga aattgctaga aatattgcgc gtgctaatca acgggatgat
     6541 ttggatttgc tcattattgg tcgtggaggt ggttctattg aggatctctg ggcctttaac
     6601 gaagaaattg tggtacgagc tatttttgaa tctcgtttgc cagttatctc tagtgtgggg
     6661 catgagacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc aacgccaaca
     6721 gcggcggctg aactggcaac acctgtgacc aagttggatg tattagctca tttgcaaaat
     6781 caggaaaaac ggatggcaac ggcagtccga aatgttctat ctaagaaaca agaggctttg
     6841 aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga cggttatttg
     6901 caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg gatttctgat
     6961 aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc tgttaccaaa
     7021 atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag ccaaatggcg
     7081 ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt gctcatgttg
     7141 gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga gtccgttgta
     7201 gattcggttg agagtttgaa gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa
     7261 gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact
     7321 ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg cgattactgc
     7381 ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca aggctgaaaa
     7441 gaccttggtc aaggtcatgc aagaagacgg aacagaaagt gattttgaat gaaaaagcaa
     7501 gaaaaattag ctcttgtcga gtcggctttg gaagattttt atggagacca gcagtttgcc
     7561 tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg tattcggcct
     7621 tttctcttgt tagaagttct ggaagccttg caggttacca tcaaacctgc tcacgcgcag
     7681 gtagctactg ccttagagat gattcataca gggagcttga ttcacgatga ccttcctgct
     7741 atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt cggtgaagct
     7801 atggccattt tggctggaga tgccttattc ttagactcat atgccttgat tgcgcaggca
     7861 gatttgccaa gtcagatcaa ggtggacttg attgccaact tatcccttgc ttcaggtagt
     7921 ctgggtatgg tggcagggca agttttggat atggagggcg aacaccagca cttgtctttg
     7981 gaagaacttc agactattca tgccaataag actgggaagt tactagccta tcccttccaa
     8041 gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa aactgtgggt
     8101 gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac agctagtttt
     8161 gaggaaatcg gcaagacacc tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc
     8221 ctgttgggct tggaagagtc cattgccttt tgtaaccaaa ccctggatca agctaatgaa
     8281 aaattggaag aaattgccca gcaggttccc tttgaaacag aatcgattgt aagtgtagta
     8341 gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata aacaggggtt
     8401 gtttgaaacg agagagcagg ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct
     8461 taatggagaa cggtttgaca agccaggaga gaaaattcca gatgacaccg aattaaaact
     8521 caagggggag aaactcaagt atgtcagccg tggtggtttg aaactggaaa aggccttgca
     8581 ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct ctactggagg
     8641 ttttaccgat gtcatgctac agaatagtgc caagttggtc tttgcagtcg atgttggtac
     8701 caatcagttg gcttggaaat tacgccaaga cccacgagtt gtcagcatgg agcagttcaa
     8761 tttccgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca gtattgatgt
     8821 gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg
     8881 tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa
     8941 tggaattatt cgagatgcta agattcatca gaatgtcctt gaatctgtaa cagctatggc
     9001 agtagaggca ggtttttcag tccttggctt ggacttttct cccatccaag gtggacatgg
     9061 aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc agattcttgc
     9121 tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat aaaaaagaaa
     9181 gacttgaaaa aattagaaga ttggtgacag attatcaaat cggcacgcaa gaagaaattg
     9241 tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc cgagatatca
     9301 aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac gaattaccaa
     9361 aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct gaattgatgg
     9421 ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa gctcagttaa
     9481 tcgatacttt tgcagacaag atttttagtt gtttgactga tgatagctcg attttagtca
     9541 ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct
     9601 atatgttact tgaaatttcg ataaaaaact ttgccattat tgaggctatt tccctcaatt
     9661 ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg attatcattg
     9721 atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt catggtgcac
     9781 caaaggcaga gattgagggg cttttctcag ttgagaatag tcgcctttta caggaaattt
     9841 ttgatgagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa
     9901 atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta
     9961 ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta atgcgtcccc
    10021 aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa
    10081 cctatcaaac gagttttgat gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga
    10141 aaaaccaaca ggaacacaag gcacgtatcg aaatgttgga atttcaaatg gcagagattg
    10201 aggcagcaaa cttgcaggct ggagaagact tggctctcaa tcaagagcga gataaactcc
    10261 tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg gacaatgaag
    10321 atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt gtcgaagagt
    10381 atgatcctga ataccgtgaa atttcaagct ctctgtctga gacctactat gttttagaag
    10441 acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc aatcgcctga
    10501 tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat ggtgggactg
    10561 ttgatgatgt tttgctttat tttgccaaga ttacggaaga atacaatctc ttgacaggca
    10621 ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc aatcttgtca
    10681 atttggcagg tcaacttgct tctgctcgtc ataatttggc tcagcaactc gaagctgaga
    10741 ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt cgttttagta
    10801 agggaaaatt cagtcgtgag ggaaataaaa tggttgagtt ttatatttca accaaccctg
    10861 gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct cgtctcatgt
    10921 tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc tttgatgagg
    10981 tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt cataaaattg
    11041 gtcagcatgg tcaggttttg gctatctccc atttgccaca agtaattgcg attgcagatt
    11101 atcaattctt tattgagaag attagtaatg accattcaac ggtttcgact gttcgtctct
    11161 tgacggtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat gatgtgacag
    11221 aagcagccct gacgcaagcc agagaattgt tgagaaacag ggagaaataa gatgacagat
    11281 tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct tctcaaaacc
    11341 tgggatggtc agccccagtt gctctttcta ggtgatttga ttgacagagg tgaggatagt
    11401 caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat ctgtttgtcc
    11461 ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag ttatgaccat
    11521 tatcgtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc cttggatgca
    11581 ccagttgatg gagtagaaga tgccaagcgg gttgccgctg aagcgtcaga cttggtcgaa
    11641 ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatatttgt tcacgcaggt
    11701 attgatttga ctttggacga ctggcatgaa acaacagatt ataaaaaagt atggcttaga
    11761 aaaccattcc acggagctga aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg
    11821 gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac agaagatggc
    11881 aagattggaa tggatggagg agctgtttat ggtggtgtcc ttcacgggat tgtctttata
    11941 gaccaaggaa tgacagaaca ccactttatc gaaaatgacg gcgttgtcgc tgaagattag
    12001 tactcctagc agggtatggt cttgtcaaaa tatcaaaaac aatttataat aaatagatac
    12061 cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacaggg gaagatccgt
    12121 aacttctcta ttatcgccca tattgaccac gggaaatcaa ctctagcaga ccgcattttg
    12181 gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga tagcatggat
    12241 ctagagcggg aacgtggaat tactattaag ttgaatgcca tcgagttgaa ttacactgca
    12301 aaagatgggg aaacttatat tttccacttg attgacacac cagggcacgt tgactttacc
    12361 tatgaagttt cacgttcgct agctgcctgt gagggtgcta ttttggtggt cgatgcggct
    12421 caaggaattg aggctcaaac tcttgccaac gtttatctgg ctttggataa tgatttggaa
    12481 atcatgccta tcattaataa aattgacctg ccggctgcag atccggagcg cgtgcgtaca
    12541 gagattgaag atgtgattgg tttggatgcc agtgaagcag ttttggcttc tgccaaggct
    12601 ggtattggga ttgaagaaat cctcgagcaa attgtagaaa aagtaccagc accaacgggt
    12661 gatgtgacgg cgccacttaa ggccttgatt ttcgactctg tttacgatgc ttaccgtggg
    12721 gttatcctcc aagtgcgtgt catggacgga gtggtcaaac ctggtgataa gattcagctc
    12781 atgagcaata gtaagacctt tgatgtggcc gaagtcggta tttttacacc aaaagcggtt
    12841 ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg cggcgtctat caagacggtt
    12901 caggatactc gtgtgggtga taccgttacc ttggcaacca atcctgcggc agaaccatta
    12961 catggttata agcagatgaa tcctatggtc tttgcgggtc tctacccaat cgaatcaaac
    13021 aagtacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag
    13081 tttgaaccag aaacatctca ggcacttgga tttggtttcc gttgtggatt tcttggactt
    13141 ctccatatgg atgttattca ggaacgttta gagcgtgagt tcaatattga cctcatcatg
    13201 acagctccgt ctgttattta caaagttaat ttgaccgacg gtgagtctat ggatgtgtct
    13261 aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc ttatgtcaag
    13321 gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag
    13381 cgtggggact ttgtgactat ggactatatt gatgataacc gtgtcaatgt tatctatcaa
    13441 attcctcttg ctgaaattgt ctttgacttc tttgataaac ttaagtcttc gacacgtggt
    13501 tatgcaagct ttgactacga attgtcagaa tatcgcccat ctaagctggt gaaaatggat
    13561 atccttctca atggagataa ggtggatgcc ctcagcttta tcgttcacaa ggactttgcc
    13621 tacgaacgtg ggaaactcat cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt
    13681 gaagttccaa tccaagcggc tattggacac aagattgtcg ctcgtactga tatcaaggcc
    13741 cttcgtaaga acgtacttgc taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc
    13801 cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca tcggatcagt tgaagttccg
    13861 caagaagcct tcctcagcgt cttgagtatg gatgaagaat aagttatcag aaatgccttt
    13921 ccttgttcac aggaaattta tataagaatc aatagtaaag caggcttaga tggtctgctt
    13981 ttttagtttt tatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttactgtat
    14041 gtatggtt
//
LOCUS       DAVLVZ010000047        13359 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000047 DAVLVZ010000000
VERSION     DAVLVZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.047"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            178..495
                     /locus_tag="VYN77_001467"
     CDS             178..495
                     /locus_tag="VYN77_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1558912.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(540..1352)
                     /gene="folK"
                     /locus_tag="VYN77_001468"
     CDS             complement(540..1352)
                     /gene="folK"
                     /locus_tag="VYN77_001468"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1558913.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(1395..1949)
                     /gene="folE"
                     /locus_tag="VYN77_001469"
     CDS             complement(1395..1949)
                     /gene="folE"
                     /locus_tag="VYN77_001469"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1558914.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(1930..3252)
                     /locus_tag="VYN77_001470"
     CDS             complement(1930..3252)
                     /locus_tag="VYN77_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1558915.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(3254..4228)
                     /gene="folP"
                     /locus_tag="VYN77_001471"
     CDS             complement(3254..4228)
                     /gene="folP"
                     /locus_tag="VYN77_001471"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1558916.1"
                     /translation="MMKILEGVTDMSSKANHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(4323..5030)
                     /locus_tag="VYN77_001472"
     CDS             complement(4323..5030)
                     /locus_tag="VYN77_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1558917.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(5083..6501)
                     /locus_tag="VYN77_001473"
     CDS             complement(5083..6501)
                     /locus_tag="VYN77_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1558918.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            6691..7503
                     /locus_tag="VYN77_001474"
     CDS             6691..7503
                     /locus_tag="VYN77_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1558919.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            7842..8861
                     /gene="adhP"
                     /locus_tag="VYN77_001475"
     CDS             7842..8861
                     /gene="adhP"
                     /locus_tag="VYN77_001475"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1558920.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSETMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            9094..10083
                     /gene="manX"
                     /locus_tag="VYN77_001476"
     CDS             9094..10083
                     /gene="manX"
                     /locus_tag="VYN77_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1558921.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            10111..10914
                     /locus_tag="VYN77_001477"
     CDS             10111..10914
                     /locus_tag="VYN77_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1558922.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            10938..11849
                     /locus_tag="VYN77_001478"
     CDS             10938..11849
                     /locus_tag="VYN77_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1558923.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(11905..13239)
                     /gene="pepC"
                     /locus_tag="VYN77_001479"
     CDS             complement(11905..13239)
                     /gene="pepC"
                     /locus_tag="VYN77_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1558924.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
BASE COUNT         3876 a         3001 c         2646 g         3836 t
ORIGIN      
        1 gtaactgaaa gttatataat caagttaatt tgggaattta cctattaact tcggtagatt
       61 tttgggaatt tttttccttc tttttccctg tagttaaaat ccttttcaaa aaatcacctt
      121 ttcttgattt ttaatcctat ttgctgtatg ataagggaaa agaaaggaga cagagatatg
      181 gcttttacca atacccacat gcggtctgct agttttggca ttgttaccag cttgcctgat
      241 gacatcattg actctttttg gtatattatc gaccatttct taaaaaatgt ctttgaattg
      301 gaagaagaac tcgagtttca attgcttaat aaccaaggaa agattacctt ccacttttca
      361 agtcaacacc tccctacagc cattgatttt gactttaacc atcctttcga ccctcgttat
      421 cccccaagag tactggtttt agacatggac ggtagagaaa ctatcctcct cccagaagaa
      481 aatgacctat tttaaaaact ctagccttca gttgcaagta actgaaaact agagtttttc
      541 tattttttca aagcatcata caagttacgg atcggttgtt ttaatatcgg atggataaaa
      601 tgaggcgcaa tttcctgtaa ggactcaagg acaaaaaggc gttccgctat gtaaggatga
      661 ggcaatatga ggtcgtctgt ataaaggatc tggtcctcca caaagagcaa gtccaaatca
      721 atcaaacgag gtccccaatg cacttctctc acccgtccca gctctgactc aatggctaac
      781 aaggtttcta acaagtcttg tgctggtagc caggtttcca cctcaaccac ttgattggca
      841 aagctatcct gctccactcc accccaaggc tccgtcgcta agacactgga ctctttgaga
      901 atatagatgc cacgagctcg cagtttgtca atggcttgct tcaagtttgc ttgtttatct
      961 cccatattgc ttcctagggc gataaaggct cgttgcttgc ggcgatgaat ggttaccgag
     1021 caagtatcta gtgacaaatg caccggtgcc caaggttttt tcagttccaa cttcatttct
     1081 tggacaagag gataagactc aaaggtacgt tccaccagtt tataggctac cgtttcaatc
     1141 aaatcttcac tcgtttcctg aaaccaagtc gtccactgct gacacaattc tccgtaatgg
     1201 acagaggctg ttaaatccaa gtctgtagct gccttggtca tatcatagga taggatggct
     1261 gaaacgacaa atttctgccc caattctttc tcactaggaa aaagaccatg ataggcaaac
     1321 atttccaaat ccttaatctg cagttgatcc ataatgattc ctttctagaa aaatccgctt
     1381 gaaacggatt ctttttatag ccccattaaa cgataagctt ggtcgcggag gtccttatct
     1441 gtttcaaata gaccacgagc tactgtcgtc aaggttgcag tgcctggttt tctaacacca
     1501 cgcatactca tacacatatg ttccgcctca atgacaacaa aggctccttt agcacctaga
     1561 tagtccatca aggcatcggc cacttcgata ttcaaacgtt cttgaatttg tggttttttc
     1621 gaataaactt caaccgtacg ggctagctta gacaagcctg ccacacgacc atctggaatg
     1681 taggcaatgt gcgctctacc ataaaatggc aagaagtggt gttcacacat ggtatggaaa
     1741 aagatatcct tttctaccac catattatcg tcaataattt caaaggattt tgacaaatgt
     1801 tcctctgctg tttgaccaag acctgaaaaa atctcttgat acatacgggc tacacgagca
     1861 ggtgtttcct gcaagccctc gcgattagcg ttctctccta cagcctcgat aatcattttt
     1921 acagctgctt caatcttttg tgtatccatt ctcgttcttc ctctccatca gataggccct
     1981 cacttggctc aagaaataca aggaacctgt gacaatccta actgtttgtt tctcttcttt
     2041 tttatctgtc aaattctgct ctagaaaatc atgccaatct tggtagctga gatttctaga
     2101 cttagctgcc tctttcagca cgctttcatc cgtcgcccga ctatccgcaa aatgtgttag
     2161 agtaagctcg gtatctggca tagctcccag caagtccaac atatccccca aggctttggt
     2221 tttgatacaa gtgaagagga tttccttatg ataatccgca aaacgttctt gcaaggttac
     2281 caacaaggcc ttgatagcat ggggattatg ggctccatcc aaaatcatca agggatctct
     2341 tgacacgatt tccaaacgcc ctggccaact tgtttcttcc aaggcttgac caagaaagtc
     2401 attgcttgct agctctcgac catcttcttg acaaaaagta tcaagtaaag ctatggccat
     2461 cccagcattc tctatttggt acaaaccaag caggctagtc tggaagcgac cttgtctgac
     2521 agcacttgta tagtcaaaga cttcccctgt caccacactt tcttgatgac gaacctgata
     2581 atctgtcccg taggcaagtc tcggcgcatc tttcccttcc gcaatgcggt caatcacagt
     2641 caaggcttct ggagcaatac gccctgttac caagggcatc ccttgtttga taatacctgc
     2701 cttctgctct gctatggcct ccaaggtgtc accaagtagg gccacatgat ccaagccaat
     2761 agttgtaatt cctgtcaaaa tgggctgaca gacattggta ctatccaaaa gtccacccat
     2821 gccaacttcc atgatagcca catctacttg ctctgaggca aagtagtcat aggctagggc
     2881 tgtgataatc tcaaactcgg ttgtgccctg taaattggcg accgcttctc cctccagcaa
     2941 agactgatag tctgccatga gagcttctag cctcgcttct gagatcgatt ccccattgat
     3001 gctaatctgg tctgtgtaat gaatgagata gggcgaacta aacacgccaa ctctcagccc
     3061 tagcttttct agcatctttt tcaaaaaagc aatagtcgag cccttgccgt tagtccctcc
     3121 gatatggaga accttgagtt taagatgggg attgccacgc aaagctaaca gttccaccat
     3181 tcgttccaag ccaaaatgcg gttgatccgt ccggtagtta gcaatccact gattgttttc
     3241 aatttctttc atcttattta tattgtttta aatctaaatt ttccgcttca tcagccagac
     3301 gaatggcaga ggcaatttca actgccatcc tgtgactagc tacgtcatgc acgcgcacca
     3361 cttctacacc ctgtctcgca gcgatactag ttacatgagc cgaagccgtg tcccgatttc
     3421 ggaaaccaag ctctgtctca ggattgactt caaacccact ttcttcaagg atattgatga
     3481 caaatcgctt gcgcgacact ccgagaaaga ttggatagcc cttctgatgt agtttatcca
     3541 ggtcccgtaa aaggagaaga ttttctttct tggtcagacc aaagccaatt cctggatcca
     3601 acaggatatt ttctggtgca ataccagctt ctgccgctct cgctagtgct cgttcaaaga
     3661 aagccaccat caagtcttcg attggcaatg tttcaaagtc agctaactct ttttctgtaa
     3721 aggtttgacc aaagccaaaa tgagggaaga taagcgaact aggatgctga ggtcgagcca
     3781 taactgggtt aaacatgatg accactttcg ctccagcttt agcaaccaca tgggccattt
     3841 tttcatcacc catgagacca gtgatatcat tgactagatc ggcaccagca gccaaagcag
     3901 cctctgctac ttgactcttc caagtatcaa tagagatgag tacatcactt tccttgcgaa
     3961 tcgctttgat cactggaaca acacgctgga tttcctcttc tatctcaaca tagctacttc
     4021 ccggccgagt cgattctccg ccgatatcga gcatactggc tccttctgct atcaatttac
     4081 gagcctgctg gagcgcctgc tcaagagcaa aaaattgacc accgtccgaa aaggagtctg
     4141 gggttacatt gataattccg caaatagctg tctttgcatg attggcttta cttgacatat
     4201 cggtcactcc ctcaaggatt ttcatcatat tatttttcta ttttaccata aaaagaaaaa
     4261 gatggacacg attgcattca tctttcccta gtagaaagag gtcggttcct acataaaaac
     4321 ttttaaacag aaattcctaa tgtccgactc ataatcacca caagagccaa caaacagaaa
     4381 gcaatcccat taacaatcat gtgaagcaag atcgacattt ccaaacgttg ggtcttgtag
     4441 gccgtccaag atagaactgt cgacatacct ccataaatca ataaagaagg taaattactt
     4501 ggttgatgca ataaagcaaa cacaatcgta ccgactacaa atcccaagtt ctccttgcct
     4561 cggaaaatct ttttaggaac aatcccacgg cacaagattt cctcacaaat cggagcaagc
     4621 aaggctagca agaagaaact ggaaatcaac gaactatttt gaaccatatc gttaatctga
     4681 gactggttag ctgttgtcgt ctcatttgac agttgcaaca agatagaacc aagtatattt
     4741 gacccgacaa taactaaata actcaagccc aaacgtgcca aatctttagc tctaaaaaaa
     4801 gaaaaattaa aactagctaa cttggtttta cgagctccca taataaatag agccagaacc
     4861 acaattgaaa ggccagcaac tatcagccct gactgtagca gggctacttc ttttaaagtc
     4921 aaaatagagg taaccactag gggaacctga tataaaagga cggcaagtaa aaagaccaaa
     4981 atccagcctg cacgattcaa caattctttc cacatatttt tctctttcat cacctatctc
     5041 ctttgtatta aaaaaggggg gaaatccccc tgggtcattc tattataagg ccatgctgat
     5101 gtagttaagg ataaacaagg catccaaaat ccaaatcatg gcatgaacat ctttaacttg
     5161 acctttgaca agcttagtca aagtgtaagt caagaaacca actgcaatcc cttgagtgat
     5221 agagtagctg aatcccataa agatagatgt gaagaaggca ggaactgctt cagacatatc
     5281 atcccaatgg atatttttca agctaccaag catcataatc ccaacgataa tcaagattgg
     5341 agctgtagcc gctgttggta cgatcgctag aagtgggcta aagaagcttg aaatcgcaaa
     5401 acagatagct acaaccaagg ctgtcaaacc agtacgtcca cctgcaccga taccagcagc
     5461 agactcaaca taagtcgtta cgtttgaagt acctgcaatg gcaccgactg tcgttccaat
     5521 caaatcagag taaagagcct tatccaattt ggctgattgg tgattttcac catttgtcgc
     5581 tacaatacca actttttcac ctgtaccgat caaggtacca attgtgtcaa aaatatctgt
     5641 caatgagaag gcaagaatgg ccatcagagt ttcaggcaag cgagctgtat ctgaaaccaa
     5701 agctcccaat ccttctgaac caagagctgc accaaagatt gtcttcaaat cttcaaaagc
     5761 tgcaccaaca tggttattag caaaatcgat actagacaaa tcaaccaaac caactgcaat
     5821 agcaagaaca gttgttgtca agatagagag aataattccc cctttaatcc ctttgatgac
     5881 aaagaagata gtaatggcaa gtcctgcaag agccactaaa acagctggat tattaaagct
     5941 gaccaatcct ggaactgctg aagagtttgc tgcaatcgtt gcttgagctt tgtcagcccc
     6001 ttctcctaca acagtatagt tgcctggatc aatcgtgaat ttcaaaagtc cagcattctt
     6061 aatccctaca taggcaagga agacaccgat accagctgaa atagctgagc gaagagcatt
     6121 gggaatcgat tcaatgatca ttttacgaac atttgtcaag gtaataatca atgaaataat
     6181 cccacagatg aagaccatag ctagggcttc ttgccaagaa taaccaagcc cgaatacaac
     6241 tgtaaaggta aagaaggcat tgagtcccat acctggcgct tgggcataag gtaagttagc
     6301 ataaaaagcc atcatcaagg tacccgctac tgcaccaata atcgtcgcta ggaagacgcc
     6361 ctgagcaggc attcctgttt gtgaaagtat ttgtgggttt acaaagagaa tatagctcat
     6421 tgcaaagaaa gttgttaaac cagcgagaac ctctgtacga acgtctgtac cgttctcttt
     6481 tagtttaaat aatttgtcca tcatcaatct ccttttaatt tttacgaaca ataataaaat
     6541 tatatcattt atcattcact ttttcaatgt ctttgtttga tttgtttaag aaattaatcc
     6601 aatttcattt tttgtttgga atctgctttt ctgcctgctt tctgttataa tagaagacat
     6661 cttatctgaa aacacactag aaaggtccat atgacgaaaa aaattattgc agttgacctg
     6721 gatggaaccc tgctcaactc agacagtcaa atttctgact ttaccaaaag aaccatcaaa
     6781 aaagttgctg aaaaaggcca tcaggttatt attacgacag gtcgccctta ccgtatgtca
     6841 aaagattttt accgtgaact gggcttagac actcctatga ttaacttcaa cggatccctt
     6901 actcatttac cagaccaagt ttgggatttt gaaaagtgtt tgactgtaga caaaaaatat
     6961 ctgctagata tggttcaacg ttcagaggac attcaagccg attttatcgc tggagaatat
     7021 cgtaaaaaat tctacattac aaatcccaat gaagaaattg ccaatcccaa actatttggt
     7081 gtagaagctt tccagcctga agatcaattc cagcctgaat tggtgaccaa ggaccctaac
     7141 tgtatcctct tgcagaccag agccagtgac aaatatgcct tggcaaaaga aatgaacgcc
     7201 ttctaccagc atcaactttc tatcaatacc tggggaggtc cgctcaatat ccttgaatgt
     7261 accccaaaag gtgtcaacaa ggcctttgct ttggactact tgctcaagat aatgaatcgt
     7321 gacaaaaaag atttgattgc ctttggagat gaacacaatg ataccgaaat gctcgctttt
     7381 gctgggaagg gttatgccat gaaaaatgcc aatccagagc tactccctta tgcagatgag
     7441 caaatttccc ttaccaacga ccaagatggg gttgccaaaa ccctacaaga cttattctta
     7501 taacctatac tgatactcaa tgagaatcaa agagcaaact aggaagctag ccgtaggttg
     7561 ctcaaagcac tgctttgagg ttgtagatga agctgacgtg gtttgaagag attttcgaag
     7621 agtatgacca gcttgcaagc tggtctttgt gctcttttca cagtctcatc aaccagttaa
     7681 tcgattgttc tactttttgt ctccaaaacc ttggaaaagg ctttcttttg tgatatactt
     7741 cacatataaa tacaagagag ctcgtcacac tcgactctgt tgacaaaaaa tcaatccagt
     7801 cccgttgtcc ctgttctcgg tcaatatcaa ggaggatagc tatgaaagct gttgttgtaa
     7861 atccagaaag cactggtgtt gctattgaag aaaaagtact ccgtccactt gaaactgggg
     7921 aagcacttgt agaagttgaa tactgtggtg tttgccacac cgacctccac gttgctcatg
     7981 gtgactttgg tcaagtccca ggacgtgttc ttgggcacga aggtatcggt atcgttaaag
     8041 agattgctcc agatgtgaaa agccttaaag tcggtgaccg cgtcagcgtt gcttggttct
     8101 ttgaaggatg tggcacttgc gaatactgta caactggtcg cgaaaccctt tgccgtacag
     8161 tgaaaaatgc tggctactca gtagacggtg gtatggctga acaatgtatc gtaactgctg
     8221 actatgctgt caaagttcct gacggacttg atccagccca agcttcttct atcacatgtg
     8281 ctggagtaac aacctataaa gctatcaaag aagcaaaagt tgaaccaggc caatgggttg
     8341 ttctttacgg tgctggtgga cttggtaacc tcgctgttca atacgctaaa aaagtattca
     8401 atgctcatgt tatcgcagtc gatatcaaca atgacaaact tgcccttgca aaagaagtag
     8461 gcgctgacat tgtgattaac ggcctcgaag ttgaagatgt agctggactc attaaagaaa
     8521 aaactgatgg aggagctcat tcagctgtcg taactgctgt gtctaaagtt gccttcaacc
     8581 aggctgttga ctccattcgt gctggtggtc gcgtcgtcgc tgttggtctt ccttctgaaa
     8641 cgatggaact cagcatcgtt aaaacagtcc tcgatggaat ccaagtcatc ggttctcttg
     8701 tcggaactcg taaagactta gaagaagcct tccaatttgg tgcagaaggt ctggtagtcc
     8761 cagttgttca aaaacgtcca gtagaagatg ctgttgccat tttcgacgaa atggaaaaag
     8821 gccaaatcca aggacgtatg gtactcgact tcacccacta atctaataga aacctatttt
     8881 aaaagttgga agcttcttcc aactttttta tattaaattt taaataagaa ttcaagatta
     8941 ctcctcaaaa actcaacata tcaacataca aaaataaaaa ttttttaatt ttttatgata
     9001 aatagttgct ttttttatga aaacggttta tacttaaaca gtcttaaagt tttcttaaat
     9061 gaaaactaaa acaaaaaagg aggaatgaca ataatgagta tcggaatcat tattgcgagc
     9121 cacggcgaat ttgctgcggg tattcatcag tcaggatcta tgatctttgg tgaacaagaa
     9181 aaggttcaag ttgtgacctt tatgccaaat gaaggtcctg atgatctata cgctaagttt
     9241 aataacgctg ttgctgcatt tgacgcagaa gatgaggttc tagttttggc tgacctttgg
     9301 agtggttctc catttaacca agctagtcgc gtgatgggag aaaatcctga gcgtaagttt
     9361 gccatcatca caggacttaa cttaccgatg ttgattcaag cctacacaga gcgcctcatg
     9421 gacgctgctg caggtgtaga aaaagtcgct gctaatatca ttaaagaagc caaagatggc
     9481 atcaaagctc ttccagaaga gctaaatcca gttgaagaag ttgcaagcgc tgcagctgct
     9541 ccagttgccc aaactgctat cccagaagga actgttatcg gagacggtaa attgaaaatc
     9601 aatcttgccc gtcttgacac acgtctactt cacggtcagg ttgcaactgc ttggactcca
     9661 gattcaaaag caaatcgtat catcgttgct tcagataacg tggctaaaga cgaccttcgt
     9721 aaagaattga ttaaacaagc agctccaggt aatgtcaagg ctaacgtggt tccaattcaa
     9781 aaactgattg agatttcaaa agacccacgt tttggagaaa cacatgccct tatcttgttt
     9841 gaaacacctc aagatgccct tcgtgccatc gaaggcggcg tgccaattaa gactcttaac
     9901 gttggttcta tggctcactc aacaggtaaa acattggtca ataccgtttt gtctatggac
     9961 aaagaagatg ttgctacatt tgaaaaaatg cgtgacttgg gtgttgaatt tgatgtccgt
    10021 aaagtaccaa atgattctaa aaaagatttg tttgacttga ttaacaaagc caatgtcaaa
    10081 taagccatta tttatgaaag gattttaaac atgtctatta tttctatggt tttagtagtc
    10141 gttgtagcct tctttgcagg tcttgaaggc atcctcgacc agttccaatt tcaccaacca
    10201 cttgtagcct gtacccttat tgggcttgta acaggtcact tggaagcagg gattatcctc
    10261 ggtggatcgc ttcaaatgat tgcccttggt tggtcaaata tcggtgctgc tatcgctcct
    10321 gatgctgcac ttgcttctgt cgctgctgcc attatcatgg ttcttggtgg tgactttacc
    10381 aagactggta tcggtgttgc ccaagcggtt gctatccctc ttgccgtagc tggacttttc
    10441 ttgacaatga ttgttcgtac aatttcagtt ggtttggttc atactgcaga tgctgccgct
    10501 aaaaaaggtg acttcggcgc tgtggagcgt gcgcatttca ttgcgctact tttccaagga
    10561 cttcgtatcg cgcttcctgc agctcttctc cttatggtac caactgaaac tgtacaaagt
    10621 atccttagtg ccatgccaga ctggctcaaa gatggtatgg ctatcggtgg tggtatggtc
    10681 gttgccgttg gttacgccat ggttatcaac atgatggcaa ctcgtgaagt atggccattc
    10741 ttcgctcttg gtttcgttct cgctgctgtg tcagatatta ctctaatcgg attcggtgct
    10801 atcggtgttg ctatcgctct tatctacctt cacctttcta aaactggtgg aaatggtggc
    10861 ggaggagccg caacttctaa cgacccaatc ggcgatatcc tagaagacta ctaagataag
    10921 aaaggactga aaacatcatg actgaaaaac ttcaattaac taaatcagat cgtaaaaaag
    10981 tttggtggcg ttcaaccttc ttacaagggt cttggaactt tgaacggatg caaaacttgg
    11041 gctgggctta tacactcatt ccagctatca aaaaactcta tactaaaaaa gaagatcaaa
    11101 tcgctgctct tgagcgtcac cttgagttct tcaacactca tccatacgta gctgctccag
    11161 tcatgggggt tactcttgcg cttgaagaag aacgtgctaa cggtgtggaa atcgatgacg
    11221 ctgctatcca aggggttaaa atcggtatga tgggacctct tgctggtatc ggtgacccag
    11281 tattctggtt tacagtacgc ccaatccttg gatctctcgg tgcttcactt gcccttactg
    11341 gcaatatctt ggggccactc ctcttctttg ttgcatggaa cttgattcgt atgtcattct
    11401 tgtggtatgt tcaagagatt ggatacaagg ctggatcaga aatcactaaa gatatgtctg
    11461 gtggtatcct tcaagatatc actaaaggag cttctatcct tgggatgttc attcttgctg
    11521 tccttgttca acgctgggta aatattaaat ttgctttcga tgtttctaaa gttcaactag
    11581 atgaaaaggc ttatatccat tgggataaat tgccagaagg gtctaaaggt atccaagaag
    11641 cattcgcaca agtaggacaa ggattgtctc aaactcctga aaaagttact actttccaac
    11701 aaaacttgga tatgttgatt cctggattat caggactact ccttacttta ctttgcatgt
    11761 acttacttaa gaaaaaagta tctccaatca ctattatcct tgccctcttc gcagtgggta
    11821 ttgtggcaca tgttcttcac atcatgtaat caagcaactg aaaaggaacc aggttctaaa
    11881 atctgattcc ttttttctat gcttttattc agccaaggct cccattggat cccatggtgc
    11941 aagtacgatt ggttctgctc cataggcagc ttgttcttct gctgtcagca attccttacg
    12001 aacaacgatt tggtatgtgt attcgtccat ccaagcgtct gaggcaacaa agtaaccatc
    12061 tgtaccgacc ttgtctcccc atgagttttc aaccttccac ttggttgatt taccattttc
    12121 gtccaagtca acacctgtca agaccatggc gtgggtcatc aagctttcac tatagtccaa
    12181 acgtccagcc ttgtcttgag taagtttaat gtccatgctt gattcaaagt cataaacatc
    12241 tgtcgcaagg atgccagcct tacggttgct gagctggccg acatcagaac caaaccaaac
    12301 agtctcacct gcttgcattt gggcaatcgc caattctttc aagcgctcca ttggaacgtt
    12361 gatgtagcga actgcacggc taccaaccac attccccaac atctcaactg tgtaagattt
    12421 tccgtaaggt ttatcagcag ttggagcatt gataacagaa acgtagtctt ctaaaggaag
    12481 attgacatat ttcttgtaaa actcttgtgg tgtgattcct ttttcacttt tgtagttgtt
    12541 atctttatcg cgataagcaa agtcaaactt gcgtggtgga agtcctaatg acatagcaag
    12601 aaagttaaag atttcttgca agaggtcttc tttcttagct tgaacagtcg cttgatctgc
    12661 accagaaaca agcaagtcac gcaagatttg agcatcttga cgaagcaatt tattaaggat
    12721 cgcatttagc tcacgactgc tgctagatga aacagactca ggataaactg acttaggcac
    12781 gacaccgtat ttttcaaaga gggaaacgac catatcccat tgaccaccat cttgttgagg
    12841 tgtttggagt aagaagctaa ccttgcggct agtcaattct tggtctgaag tcgcaatgac
    12901 ttgctccaag aaccagtttg atttctcata cttatcccag aagaaagtgt gggcttgtga
    12961 caactcaaag ttctccaatt tgtattgcga gatgagtttg tggcggaagg tgttgagagc
    13021 cgcaaacatc cagcaacgac cagacgcttt ctggttagtg accttgtcct tggttaaatc
    13081 caatgagaaa acaggtgtgt tgtctacatg gctttggcga cattccagag ctgcaaaaat
    13141 tccgttgtgg ctggcagcat tttcaatcgc ttggtatttt acatttgctt catagttggc
    13201 aaatagttta tcagtaaatg attcttgaat cgcgttcata gattcctcct tttattctac
    13261 agtctattat aactcttttc acaaaggaag caactaaaaa acataaaagg taaggacatt
    13321 ctccctcacc tttttcgaat ttatactctt cgaaaatca
//
LOCUS       DAVLVZ010000048        13088 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000048 DAVLVZ010000000
VERSION     DAVLVZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.048"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            51..914
                     /locus_tag="VYN77_001480"
     CDS             51..914
                     /locus_tag="VYN77_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1558925.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1297..1917
                     /locus_tag="VYN77_001481"
     CDS             1297..1917
                     /locus_tag="VYN77_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558926.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            2003..2446
                     /gene="tsaE"
                     /locus_tag="VYN77_001482"
     CDS             2003..2446
                     /gene="tsaE"
                     /locus_tag="VYN77_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1558927.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2436..2954
                     /locus_tag="VYN77_001483"
     CDS             2436..2954
                     /locus_tag="VYN77_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1558928.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2962..3978
                     /locus_tag="VYN77_001484"
     CDS             2962..3978
                     /locus_tag="VYN77_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1558929.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4063..5319
                     /locus_tag="VYN77_001485"
     CDS             4063..5319
                     /locus_tag="VYN77_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1558930.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5374..6540
                     /gene="recA"
                     /locus_tag="VYN77_001486"
     CDS             5374..6540
                     /gene="recA"
                     /locus_tag="VYN77_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1558931.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            6847..8217
                     /locus_tag="VYN77_001487"
     CDS             6847..8217
                     /locus_tag="VYN77_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1558932.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8593..9549
                     /gene="lytA"
                     /locus_tag="VYN77_001488"
     CDS             8593..9549
                     /gene="lytA"
                     /locus_tag="VYN77_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1558933.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADRPEFTVEPDGLITVK"
     gene            9908..10081
                     /locus_tag="VYN77_001489"
     CDS             9908..10081
                     /locus_tag="VYN77_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1558934.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLYLSLNQSMIAK
                     MEKGERNITNGFK"
     gene            10068..10274
                     /locus_tag="VYN77_001490"
     CDS             10068..10274
                     /locus_tag="VYN77_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558935.1"
                     /translation="MDLSNKASNLRKKLGADGEAPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            10320..10679
                     /locus_tag="VYN77_001491"
     CDS             10320..10679
                     /locus_tag="VYN77_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558936.1"
                     /translation="MKKSSVSLILIGEGDETERKADQLASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLG"
     gene            10808..11233
                     /locus_tag="VYN77_001492"
     CDS             10808..11233
                     /locus_tag="VYN77_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558937.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDNNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKA"
     gene            11641..12435
                     /locus_tag="VYN77_001493"
     CDS             11641..12435
                     /locus_tag="VYN77_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558938.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
     gene            12439..12636
                     /locus_tag="VYN77_001494"
     CDS             12439..12636
                     /locus_tag="VYN77_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558939.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            12626..>13088
                     /locus_tag="VYN77_001495"
     CDS             12626..>13088
                     /locus_tag="VYN77_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558940.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCG"
BASE COUNT         4155 a         2088 c         2836 g         4009 t
ORIGIN      
        1 ctaaaaactt tataatgaaa ttaagcaaat aggaaggttt attatcttta atgaatacac
       61 tcgcagagaa attcagattg aaaagaaaag agttgagact ctcccaacaa actcttgcag
      121 aaggaatttg tgaacaaagc cagattagta aaattgaaag aggtcatttt attccctccg
      181 cagacttgtt attcaaactc tctcaacgac ttgaagtccc tttggattac tttttcaatg
      241 aacaaatcga agttaagtct aacctctcta atttcaaaca tctatctgct cgcctattag
      301 atgacagaaa ttatgacgat ttagaatatc tttataaaat agaggtagaa cgaagcactt
      361 ttctaacatt agaagaccga acttaccttg aatggattaa atctataatt gacttctatc
      421 aatatgacag taagtgtgag gcaatttcgt cattggaaaa tatattatta aaagtctctt
      481 caaatactct gatttattta aaggcgttga atactctatc taatttctat tccttagtgg
      541 gtcgtgaaca agaatatgag gcaaactact ctcatttaat agagttgtat cagacaaaaa
      601 atttggacca tcaagagttt ttatttggct acatcagagt tcgttacaac tacgctcact
      661 acctagtatc aaaggaaaaa tataatgaag ccatccaaga agctcttgaa acgattgaac
      721 tctgtaaaca aagacagaca agctaccaat tggctcctct acttattctt gtaggaaatg
      781 ctggagccaa atttctagac agagaacaag tcaaaaatta ttatatagaa gcaagagagt
      841 tatgtaagat ttataacaat cctttaatgt tgatgaagat agaaaattat ttgaaggaac
      901 tagatactgt ttagttaatc ttgacattat agtttttctg aaacgttaaa taacctgtaa
      961 ggctgatagt tagattaata ctatcagcct tataattatg tgactctgag tccgtctcgc
     1021 tccgtgaggt gcgagacaaa acaaccaccc gtttgacgga ttttcatgat ttcaaattta
     1081 tattcttcga aaatctcttc aaatcacgtc agctctatct gcaatctcaa aacactgttt
     1141 tgagcaacct gtggctagct acctagtttg atctttgatt ttcattgagt attagtctct
     1201 agtgtttgtt tagcgtctta aagagacatt tgagaagatt agaaacagtt ctctcttttc
     1261 tcctagtata atgattggta ggaagggaga gagaagatga aatcaatgag aatcttattt
     1321 ttgttagctt taattcaaat cagtttgagt agctgtttcc tatggaagga atgcatcttg
     1381 tcctttaaac aaagtacagc ttttttcatc ggaagcatgg ttttcgtttc aggaatctgt
     1441 gctggagtaa attatcttta tacccgtaag caagaagtcc atagtgtcct agccagtaag
     1501 aagtcggtga agctttttta cagtatgtta ctcttaatta atttgttagg agctgttctt
     1561 gttttgtcag ataacttgtt catcaaaaat acgctgcagc aagaattagt tgacttttta
     1621 ttgccatcct tctttttcct atttgggcta gatttgctga tttttttacc cttgaaaaaa
     1681 tacgtgcgcg attttcttgc tatgctggac agaaaaaaga cagtgttggt gactatttta
     1741 gcaacacttc ttttcttaag aaatccaatg accattgtct cacttctgat ttatattgga
     1801 ctgggcttgt tttttgcagc ctatcttgtc ccaaattcgg ttaagaagga agtttccttt
     1861 tatggtcata ttttccgaga tcttgtattg gtcattgtta cgctcatttt cttttagaga
     1921 aagcctgttt tttatggatg ttatgatgat aagtttagta ggaaaataga cacaaaagaa
     1981 aagtttttgg tataataaga gtatgtacac aaaaaatgaa gaagagttgc aagccttagg
     2041 ggagcgtttg ggccatctat tagcaaagaa tgatgtttta atcttaactg gagaactggg
     2101 tgcaggtaaa acgaccttta ctaaaggact tgcaaaagga ttacagattt ctcaaatgat
     2161 taaaagtccc acctatacta tcgtgagaga gtatgaaggt cgacttccac tttatcacct
     2221 agatgtttat cgtattgaag gagatgctga ttctatcgac ttggatgagt ttatctttgg
     2281 tggcggcgtg actgttattg agtggggaaa tctcttagga gatgccttgc cagatgctta
     2341 tttagaattg gaaattctaa aagaagcaga tggacgccgt ttaaattttc aggcaaaggg
     2401 tttgcgtgct gagaaattgt tagaggagct tcaatatgga gtatgaattg ctcattaggg
     2461 aagcagagcc caaagatgca gctgaattag tggccttttt aaatcgtgtg agtttggaga
     2521 cagactttac cagcctagac ggagatggta ttctcttgac tagtgaggag atggaaatat
     2581 tcctcaacaa gcaagctagt tcggacaatc agataacctt acttgcattc ttaaatggca
     2641 aaattgctgg tattgtaaat attacagctg atcagcgtaa gagagtccgt catattggag
     2701 atctcttcat tgtgattgga aaaagatatt ggaataatgg cttgggaagt ttgttgctag
     2761 aagaagcgat agagtgggca caagcaagtg gcattctgcg tcgtctccaa ctgactgtcc
     2821 aaactcgtaa tcaagcagca gtccatcttt atcaaaagca tggctttgtc attgaaggta
     2881 gccaagagcg tggggcatat atagaagaag ggaaatttat cgatgtttac ttgatgggta
     2941 aactgatagg ttagtagaaa tatggttaaa aaaattattg gaatggtgct agctttactt
     3001 tctgtaactg tagtaggagt aggtgttttt gcttatacta tttatcaaca agggacagaa
     3061 accttagcta aaacctataa aaaaatcggt gaagaaacca aggttattga agcgactgaa
     3121 cctctaacca ttctgttaat gggagtggac accggaaatg ttgaacgaac tgaaacttgg
     3181 gtcggtagaa gtgatagcat gatcttgatg acagtgaatc ctaaaacgaa aaaaacaaca
     3241 atgatgagtt tagagcggga tattctgacg cgcattgaat cagggaatgg tcaggctcat
     3301 gaagcgaaac tgaactcagc atatgcagat ggtggagcag agcttgctat agaaaccatt
     3361 caaaaaatga tgaatatcca tattgatcgc tatgtgatgg tcaatatgag agggttgcaa
     3421 aaattagtgg atgcagtagg aggtattaca gtcaataata tcctaggttt cccaatttct
     3481 atcagtgacc aagaagaatt taataccatt tctatcggtg ttggggagca acatattggg
     3541 ggagaagaag ccctagtcta tgcacgaatg cgttaccaag atcctgaggg ggattatggt
     3601 cgtcaaaaac gtcaacgtga agttattcaa aaagtcatgg aaaaagctct cagtttaaat
     3661 agcattggtc attatcaaga gattctaaaa gctttgagtg acaatatgca gaccaatatt
     3721 gatttgtctg caaaaagtat ccctaacttg ctaggctata aagattcatt taaaaccatt
     3781 gaaactcagc agttgcaggg tgaaggagag atacttcaag gtgtttctta ccagattgtt
     3841 tcgagagcac atatgttgga aatgcaaaat atactccgac gttctttggg acaagaagaa
     3901 gttactcagc ttgaaaccaa tgcggtttta tttgaagatt tatttggcag agcacctgtt
     3961 ggtgatgaag ataattaact tttgatacaa ataaaaaaat caatcgtagg aaaattcctg
     4021 cgattttttc aaaaaaatac gaatagatag gtaggaggaa acatgaaagc agaaatcatt
     4081 gctgttggaa cagagatttt gacaggacag attgtcaaca ccaatgccca gtttttgtcg
     4141 gaaaaactag ctgagattgg ggtagatgta tattttcaga cggctgtagg agacaatgaa
     4201 gttcgtctct tgtctttgct tgagattgcc agtcaacgta gcagtctggt gattttgaca
     4261 ggcggtttgg ggccaactga ggacgaccta accaaacaaa ccctagctaa atttttaggg
     4321 aaagcattag tctttgatcc tcaggctcag gagaagttgg atatcttttt tgccctgcga
     4381 ccagactatg cccgaacacc gaataacgaa agacaagctc aaattgtaga aggagcgatt
     4441 ccactgccaa acgaaacagg actggctgtt ggaggaaaat tagaagtaga cggagtgacc
     4501 tatgtcgtcc ttccaggtcc gccaagtgaa ttgaaaccca tggtcttaaa ccaacttcta
     4561 cccaagttga tgacagggag caagctgtat tcccgagttc ttcgtttctt tgggattggc
     4621 gagagccagt tggttacgat tttggctgat ttaattgata atcagatcga tcctaccttg
     4681 gccccttatg ccaagacagg agaagtcact ctacgtctgt caacaaaggc tagcagtcaa
     4741 gaagaggcga atcaagcgct ggatatcttg gaaaatcaaa tcttggactg ccagactttc
     4801 gaaggaattt ctttacgaga cttttgctat ggttatgggg aagaaactag tttagccagc
     4861 attgtggtag aagaactgaa aaggcaaggg aaaaccatcg cggctgcaga gagtttgacg
     4921 gcaggtcttt tccaagctac cgtggcgaat ttttctggag tttcaagtat atttgagggt
     4981 ggttttgtga cctatagctt ggaggaaaaa tcaaggatgt tggatattcc tgccaagaat
     5041 ttggaagaac atggtgtggt gtctgaattt acagctcaga agatggctga gcaggcacga
     5101 agcaagaccc agtctgattt tggaattagt ttgactggag tggcaggacc agatagccta
     5161 gaaggacacc cagttgggac agtcttcata ggcttggcgc aagatcaagg aactgaggtt
     5221 atcaaggtta atattggagg cagaagccga gcagatgtac gtcacattgc ggttatgcat
     5281 gcctttaacc tagttcgcaa ggctttatta agtgactaac ttttgatata atagtagata
     5341 ggtctgagga tcattagaat gtaggagaat agaatggcga aaaaaccaaa aaaattagaa
     5401 gaaatttcaa aaaaatttgg ggcagaacgt gaaaaggcct tgaatgacgc tcttaaattg
     5461 attgagaaag actttggtaa aggatcaatc atgcgtttgg gtgaacgtgc ggagcaaaag
     5521 gtgcaagtga tgagctcagg ttctttagct cttgacattg cccttggctc aggtggttat
     5581 cctaagggac gtatcatcga aatctatggc ccagagtcat ctggtaagac aacggttgcc
     5641 cttcatgcag ttgcacaagc gcaaaaagaa ggtgggattg ctgcctttat cgatgcggaa
     5701 catgcccttg atccagctta tgctgcggcc cttggtgtca atattgacga attgctcttg
     5761 tctcaaccag actcaggaga gcaaggtctt gagattgcgg gaaaattgat tgactcaggt
     5821 gcagttgatc ttgtcgtagt cgactcagtt gctgcccttg ttcctcgtgc ggaaattgat
     5881 ggagatatcg gagatagcca tgttggtttg caggctcgta tgatgagcca ggccatgcgt
     5941 aaacttggcg cctctatcaa taaaaccaaa acaattgcca tttttatcaa ccaattgcgt
     6001 gaaaaagttg gagtgatgtt tggaaatcca gaaacaacac cgggcggacg tgctttgaaa
     6061 ttctatgctt cagtccgctt ggatgttcgt ggtaatacac aaattaaggg aactggtgat
     6121 caaaaagaaa ccaatgtcgg taaagaaact aagattaagg ttgtaaaaaa taaggtagct
     6181 ccaccgttta aggaagccgt agttgaaatt atgtacggag aaggaatttc taagactggt
     6241 gagcttttga agattgcaag cgatttggat attatcaaaa aagcaggggc ttggtattct
     6301 tacaaagatg aaaaaattgg gcaaggttct gagaatgcta agaaatactt ggcagagcac
     6361 ccagaaatct ttgatgaaat tgataagcaa gtccgttcta aatttggctt gattgatgga
     6421 gaagaagttt cagaacaaga tactgaaaac aaaaaagatg agccaaagaa agaagaagca
     6481 gtgaatgaag aagttccgct tgacttaggc gatgaacttg aaatcgaaat tgaagaataa
     6541 gctgttaaag cagtggagaa atccgctact ttttcgattt ttgattcaag tttttagatt
     6601 atatatagta gcttgaaata agatatgaac aactttatta ggaaagtcaa attaatttct
     6661 agaaatgttt tagcagctac agcgtactat tccaaactca accaactata atagatcgaa
     6721 actagaatag tacacatcta cttctaaaac attgttaaaa atcgatttga ctgtccttat
     6781 ttcattccgc tatatataag tttgctgttt cttgtcgctc ctctggaaag ctgatataat
     6841 agctttatga ataaaaaacg aacagtggac ctgatacatg gtccgattct tccctcgctc
     6901 ttaagcttca cctttccaat cttgctatca aatatttttc aacagctcta taacactgct
     6961 gatgtcttga ttgttggacg atttcttggt caagaatcct tggctgcagt aggagcgacg
     7021 acagcgattt ttgacctgat tgtaggcttt acacttggtg ttggcaatgg catggggatt
     7081 gtcattgctc gttattatgg ggctcggaat ttcactaaaa tcaaggaagc agtagcagcc
     7141 acctggattt taggtgctct tttgagcatt ctagttatgt tgctgggctt tcttggcttg
     7201 tatcctctct tgcaatactt agatactcct gcagaaattc ttcctcaatc ttatcaatat
     7261 atttctatga ttgtgacctg tgtaggtgtc agctttgctt ataatctttt tgcaggcttg
     7321 ttgcggtcta ttggtgacag tctagcagcc ctgggatttc tgattttctc tgccttggtt
     7381 aatgtggttc tggatctcta ttttattacg caattgcatc tgggagttca atccgcagga
     7441 cttgctacca ttatttcgca aggtttatca gcggttctct gcttttatta tattcgtaaa
     7501 agtgtgccag aactcttgcc acagtttaaa catttcaaat gggacaaaag cttgtacgcg
     7561 gatctcttgg agcaaggttt ggctatgggc ttgatgagtt caattgtatc tatcggcagt
     7621 gtgattttac agttttctgt taatacattt ggtgcagtga ttattagtgc ccagacggca
     7681 gctcgacgca ttatgacctt tgcccttctt cctatgaccg ctatttctgc atcaatgacg
     7741 acctttgctt ctcagaatct aggagctaag cgacctgacc gtattgttca aggtcttcga
     7801 atcggcagtc gtttaagtat atcctgggca gtttttgttt gtattttcct cttttttgcc
     7861 agtccagctt tggtttcctt cttggctagt tcgacagatg gttacttgat agaaaatgga
     7921 agtctctatc tgcaaatcag ttcaaccttt tatcccattt tgagcctctt gttgatttat
     7981 cgcaattgct tgcagggctt ggggcaaaag atccttcctc tagtttctag ctttattgaa
     8041 ctaatcggaa aaatcgtttt tgtggttttg attattcctt gggcaggata taagggtgtt
     8101 atcctttgtg aacctcttat ctgggttgcc atgacagttc aactgtactt ctcattattc
     8161 cgtcatccct tgataaaaga aggcaaggca atcttggcaa ccaaagtgca atcctagttg
     8221 gatttactga ataaaatcca tttcctctag tgaaaatcga aaaaacttgt gttctcgtct
     8281 ttagtttggt gttgaaaata gtttaacaga cttttgactt cttttatatg atataataaa
     8341 gtatagtatt tatgaaaagg acatatagag actgtaaaaa tatacttttg aaaagctttt
     8401 tagtctgggg tgttattgta gatagaatgc agaccttgtc agtcctattt acagtgtcaa
     8461 aatagtgcgt tttgaagttc tatctacaag cctaatcgtg actaagattg tcttctttgt
     8521 aaggtagaaa taaaggagtt tctggttctg gattgtaaaa aatgagttgt tttaattgat
     8581 aaggagtaga atatggaaat taatgtgagt aaattaagaa cagatttgcc tcaagtcggc
     8641 gtgcaaccat ataggcaagt acacgcacac tcaactggga atccgcattc aaccgtacag
     8701 aatgaagcgg attatcactg gcggaaagac ccagaattag gttttttctc gcacattgtt
     8761 gggaacggtt gcatcatgca ggtaggacct gttgataatg gtgcctggga cgttgggggc
     8821 ggttggaatg ctgagaccta tgcagcggtt gaactgattg aaagccattc aaccaaagaa
     8881 gagttcatga cggactaccg cctttatatc gaactcttac gcaatctagc agatgaagca
     8941 ggtttgccga aaacgcttga tacagggagt ttagctggaa ttaaaacgca cgagtattgc
     9001 acgaataacc aaccaaacaa ccactcagac cacgttgacc cttatccata tcttgctaaa
     9061 tggggcatta gccgtgagca gtttaagcat gatattgaga acggcttgac gattgaaaca
     9121 ggctggcaga agaatgacac tggctactgg tacgtacatt cagacggctc ttatccaaaa
     9181 gacaagtttg agaaaatcaa tggcacttgg tactactttg acagttcagg ctatatgctt
     9241 gcagaccgct ggaggaagca cacagacggc aactggtact ggttcgacaa ctcaggcgaa
     9301 atggctacag gctggaagaa aatcgctgat aagtggtact atttcaacga agaaggtgcc
     9361 atgaagacag gctgggtcaa gtacaaggac acttggtact acttagacgc taaagaaggc
     9421 gccatggtat caaatgcctt tatccagtca gcggacggaa caggctggta ctacctcaaa
     9481 ccagacggaa cactggcaga caggccagaa ttcacagtag agccagatgg cttgattaca
     9541 gtaaaataat aatggaatgt ctttcaaatc agaacagcgc atattattag gtcttgaaaa
     9601 agcttaatag tatgcgtttt cttgtggaga tatttccttc aatgtttgct actatattaa
     9661 acaaaaatca gaaagcaaac tagaaagtta tgctcaaata aaatctaaat ttgacaatgt
     9721 aaaccgagtc ggatagcttt aagtactgtt ttgaggttga agatacgatt tttgatagga
     9781 actcatcaat tttagatttt taagcagcat caataaattg cttccttgtt ttgtcataat
     9841 ttttttattt aaaaaattat gacaaaagtg tgctattctt tttatgagag gtgtatgaat
     9901 atgataaatg tatgtgataa atgtatgtga tgttggaaaa agaataaaag aacttagaat
     9961 atcttcaaat cttactcaag ataagattgc tgaatatttg tctttatatt tgtctttgaa
    10021 tcaaagcatg attgccaaaa tggaaaaagg tgaaaggaat atcacgaatg gatttaagta
    10081 ataaagcttc aaatcttaga aaaaagttgg gagctgatgg tgaagcgccg atagatattt
    10141 ttaaattggt acaaaagata gaaaatttga cgctggtatt ttatggactc ggaaagaata
    10201 ttagcggagt ctgttataaa ggaactcagt tcagtctcat tgcagtcaat tcagacatgt
    10261 cattaggaag gtaaagattt tctttagcac atgaactgta tcatctttat tatgatgagg
    10321 tgaagaagag ttcagtcagt cttatcttga ttggtgaagg agatgaaact gaaagaaaag
    10381 cggatcagct tgcttcttat tttttaattt tcccatcttc actgtatagg atggttgagg
    10441 aaatcagaga aaatgccaat agaactcatc ttgaagtaga agatattata aaattgggtc
    10501 agttttatgg tatcagtcat aaagctatgt tatatagatt gaggaatgat ggataccttg
    10561 atgcagaaga aattaaaaat atggatatta gtgttataga gacagcttca agattaggct
    10621 atgatacaag tttatatcgt cctttgtcag aaagtaaaaa ggaaatggta ttgggataat
    10681 atattaattc aactgaacaa cttttagaaa ataacagaat ttcgcaaggg aagtatgagg
    10741 aactgttact agatgctttc agatatgata ttgtatatgg gctagatgaa gaggggggag
    10801 ttgtcgtttg actagtcgtg tatttattga tgcagattgt atttcagtat ttttatgggt
    10861 tggcactgaa catcttttag aaaagctcta ttcgggtaaa attgttattc cacaagaggt
    10921 gtatgatgaa atcaatatac ctacaattcc ccatttaaaa tctaggatag atcagttggt
    10981 agctaagggt tcagctgaga ttgtgagcat agacattgga actgaagaat acgcattata
    11041 tagagattta acaagaaatc atgataataa caagattatt ggtaagggag aagcggcttc
    11101 tatttcctta gcgaaaaagc ataatgggat attaggaagt aataacctaa gagatgttaa
    11161 atcatatgta gaagaatttt ctttagaaca tatgacaaca ggagatatac tgattgaagc
    11221 gtttaaagcg taatttatta ctgaacaaga gggcaatcat atctggaata atatgcttaa
    11281 aaagagaagg aaaattggtg caaattcatt ttcagactat cttcgtggaa gtattcatca
    11341 aaatagacaa aaataaattt ggataaatcg aactcactat tcaggaggca tatgagcaat
    11401 tcgaaaaaga aaagtgtcaa attgagccta taggagtaga agtgaaatag taagtcctgc
    11461 atagtggatg agagaaaagt tctccttgaa gttttcctga actatcagtc gcatgtcaaa
    11521 cgatatgtag ggtaatgtga gaggggatag cgagtagttt ttggttattt tatcaaaaaa
    11581 cttatatttt attataccga atgataaaat ataataaaaa tgatagaata aggaaaaaac
    11641 atgaatgtca aaaagataat gtcaattttt caatcctttt atgttgatgt cagtattgag
    11701 gaactgactt tgactttacc aatcagtttt gtaaaaaggt ttgagtatac tcaaatgact
    11761 tttcataagg aatcattttt attgattaaa gaaaagagaa gggggagttt gagttcattt
    11821 gttactcagg ctcgcactat gggtgaaaaa gccaatatgg atgttgtttt ggtgttttcg
    11881 aagttatcag acagtgaaaa aaagcaatta cttcaagcta gagttccgtt tgtagacttt
    11941 aagggaaacc tcttcttccc tccattggga ctagtactca atgcgaatga tactgaagtc
    12001 cctaaggaat taacacctag cgaacaatta acgtggattg cctttttatt gacaaaaggt
    12061 caaaaagtag tagatgttga tttgctttca caagtcactg gacttccaaa ctcaacaatt
    12121 tataggtgtt tgaggacttt taaagcttta tattggttaa acaagcaaaa taagctttac
    12181 acatatacgg tgtcaaagaa agaattattc ttaaaatccg tgtcatgttt atttaatccc
    12241 atcaaaaaac ggattttatt gccagatggc gatataaagc agataaaatc tgtttctaac
    12301 cttctatatg gtggtgctta tgctttgtcg cattcaactt ttttagctga aacggatgaa
    12361 aatattagct atgtcatatg gcagagaaaa ttcaatcagt tatccttgcc actttctcag
    12421 catgttttaa aatgaaagat gctagagata tggaaatatc gtccttttgt atctgagttt
    12481 tggaatgatt ttaaaaataa tcatgataaa caatttgtag atccgatttc tctttatttg
    12541 accttaaaag atgatgatga cccacgtata gaggaagaga gtgaagcact agaaaatatg
    12601 atattacagt atctgggaga agatgatgcc agctaatacg aaagttattt ttcaagaaat
    12661 gtttgcggat tttcagaact attatgttct gattggggga actgctacct ctatcgtatt
    12721 ggattcgcaa ggatttaaaa gtcgcacaac aaaagattat gatatggtca tcattgatga
    12781 agtaaaaaat aaggaatttt atactacctt gaatcatttt ttagaattgg gagagtatca
    12841 aggaagtcag aaagatgaga aagcgcagct ttttcgattt acaacaacta attctgagtt
    12901 tccttctatg attgaactat ttagtatctt accagaatat ccattaaaga aggacggtcg
    12961 agaaattccc ttatattttg accaagatgc tagtttatca gccttattat tggatgaaga
    13021 ttattataat atattggtgc atgtaaaaga aaccattcag gggtattcgg tattgagtaa
    13081 ttgtggtc
//
LOCUS       DAVLVZ010000049        12896 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000049 DAVLVZ010000000
VERSION     DAVLVZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.049"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(54..584)
                     /locus_tag="VYN77_001496"
     CDS             complement(54..584)
                     /locus_tag="VYN77_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEV1558941.1"
                     /translation="MLDEKRKNYVEDKRQNFPSLPNWKMDLFSEEEKRNYFQTTCEIS
                     SKDSAINKYKMEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDVEGEWTALDDI
                     KNEAHMLKKLADKSYTTNLTDEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LDNIVNIVSDIHKLGI"
     gene            complement(630..1046)
                     /locus_tag="VYN77_001497"
     CDS             complement(630..1046)
                     /locus_tag="VYN77_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558942.1"
                     /translation="MDLLEKECLKCDKNFQQGDIWNYYYLSDKVPAQGWKIHISSQIK
                     DAVNIFKIVYKLSQLNNCSFKVVKNLEELKKINSPREMSPTANKFITLYPKSESEAKS
                     MICNLTNRLSEFKAPKILSDYQCGMHSLVHYRYGAF"
     gene            1595..2272
                     /locus_tag="VYN77_001498"
     CDS             1595..2272
                     /locus_tag="VYN77_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1558943.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            2259..3056
                     /locus_tag="VYN77_001499"
     CDS             2259..3056
                     /locus_tag="VYN77_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1558944.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQVSTNPFRHI"
     gene            3073..4176
                     /locus_tag="VYN77_001500"
     CDS             3073..4176
                     /locus_tag="VYN77_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1558945.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EEALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            4477..5496
                     /gene="galE"
                     /locus_tag="VYN77_001501"
     CDS             4477..5496
                     /gene="galE"
                     /locus_tag="VYN77_001501"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1558946.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            5498..6463
                     /locus_tag="VYN77_001502"
     CDS             5498..6463
                     /locus_tag="VYN77_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1558947.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(6514..6720)
                     /locus_tag="VYN77_001503"
     CDS             complement(6514..6720)
                     /locus_tag="VYN77_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1558948.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            6772..7248
                     /locus_tag="VYN77_001504"
     CDS             6772..7248
                     /locus_tag="VYN77_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1558949.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            7257..7928
                     /locus_tag="VYN77_001505"
     CDS             7257..7928
                     /locus_tag="VYN77_001505"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1558950.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            8058..8396
                     /locus_tag="VYN77_001506"
     CDS             8058..8396
                     /locus_tag="VYN77_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1558951.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIHIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            8399..9028
                     /locus_tag="VYN77_001507"
     CDS             8399..9028
                     /locus_tag="VYN77_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1558952.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            9268..10419
                     /locus_tag="VYN77_001508"
     CDS             9268..10419
                     /locus_tag="VYN77_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1558953.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGARLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            10737..11486
                     /gene="truA"
                     /locus_tag="VYN77_001509"
     CDS             10737..11486
                     /gene="truA"
                     /locus_tag="VYN77_001509"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1558954.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQIDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            11476..12249
                     /locus_tag="VYN77_001510"
     CDS             11476..12249
                     /locus_tag="VYN77_001510"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1558955.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            12227..12688
                     /locus_tag="VYN77_001511"
     CDS             12227..12688
                     /locus_tag="VYN77_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1558956.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            complement(12717..>12896)
                     /locus_tag="VYN77_001512"
     CDS             complement(12717..>12896)
                     /locus_tag="VYN77_001512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558957.1"
                     /translation="PKVRFFLSNFWGAVHSTPDTMRFSYLKDFLLNLFMLNENQTRKL
                     AAGCSKQCFEVADGN"
BASE COUNT         3682 a         2404 c         2846 g         3964 t
ORIGIN      
        1 gggggcagta cattttggta tatataaaat ttgtaggagc tatatctaca attttatatt
       61 cccagtttat ggatgtcact tactatattc acaatgttat ccagtgtttt ttctctaata
      121 tttaaggagt gttctgtttc tcgaataaat tcttcaaagt ttaacccgtc aacttgttcc
      181 tgaacaagaa aataatcatc cacgatataa aattcatcag ttaaattagt agtataactt
      241 ttatcggcta atttttttag catgtgagct tcatttttta tatcatcaag agctgtccat
      301 tctccttcaa catcataatt cacaaaaggt cttgactgct tgatgattac tttttgcccg
      361 tccgattttc taattgcccg ataaacattt cctttatttg atctcttaat aattttttcc
      421 attttgtatt tatttattgc agagtcttta cttgaaattt cacatgtggt ttgaaaataa
      481 ttcctttttt cttcttctga aaataaatcc attttccagt tagggagact agggaaattt
      541 tgtctcttat cttctacata gttcttcctt ttttcatcta gcaataaata gatgactttt
      601 ttattttttt catcataagc ttgttttttt taaaaagccc catatctata atgaactaga
      661 gaatgcattc cacattgata gtcagatagt atttttggag ccttaaattc tgacagtcta
      721 ttcgtaagat tacaaatcat actcttagct tcagattctg acttaggata tagagttata
      781 aatttgttag cagtagggct catttcccta ggggaattaa ttttttttaa ttcctctaaa
      841 tttttaacga ctttaaagct acaattattt agttgggata gtttatacac aatcttaaaa
      901 atatttacag cgtcttttat ttgggagctt atgtgtattt tccacccttg tgcaggcacc
      961 ttatctgata aataatagta attccaaata tcaccctgtt ggaaattttt atcacatttt
     1021 aaacattctt tctctaataa atccataaca aactcctaat tattaaaata ggctagagac
     1081 acagcactag cctattccgt aagtaaagct acttatttta tataaaatat aactagcgac
     1141 ctgtaattaa acaagtaaaa gaacaatact tagggagacc tgtgacaatg acatcttcct
     1201 ctggaagtag ttctaattca aacaatgttt gttccatgac tgtatcctcc tgttatgata
     1261 aattccagaa agcatagagc aaattcgtgg ttcgtctgac taaatttctt atctgaacac
     1321 caccttcaaa tacaaatttt agtcaaactt ttattttttc taactttcta attatattct
     1381 accatgtgtt tttatatgtc aagaattttc tgaataaact gtttattttt tattttttga
     1441 catttttgca aattgtttaa aatctttcaa ttggattcac ttattacttt aggacattga
     1501 aacttctttc gccagaggtg cttatattac tgggaagttg aagattgaaa tgtggtataa
     1561 taagaggtaa tagagttttg gaaagtgaga gaagatgatt tcaaagagat tagaattggt
     1621 agcttccttt gtgtcacagg gggctatttt actagatgtg ggaagtgacc atgcttatct
     1681 gcctatcgag ttggttgaga gaggccaaat caaaagcgct attgcaggtg aggtggtgga
     1741 aggtccctat cagtctgcgg ttaaaaatgt tgaggctcac ggcctaaagg agaaaatcca
     1801 agtccgttta gccaatggct tggcagcttt tgaagagact gaccaagtgt ctgtcattac
     1861 cattgctggc atgggtggtc gtttgattgc taggatttta gaagaaggtt tggggaagtt
     1921 agctaatgta gagcgtttga tcctccagcc caataatcgt gaagacgact tgcgtatctg
     1981 gctacaggat catggattcc agattgtagc agaaagcatc ttagaagaag ctggaaagtt
     2041 ttatgagatt ttggtggtgg aagcaggaca aatgaagcta tcagccagtg atgttcgctt
     2101 tggtcccttc ttgtccaaag aagtcagtcc agtatttgtc caaaaatggc aaaaagaagc
     2161 tgagaagcta gagttcgccc tcggacaaat cccagaaaaa aatctggaag aacgtcaagt
     2221 tctagtagat aagattcaag ctatcaagga ggtgctccat gttagcaagt gaagtgatcc
     2281 aagcttatga agccttttgc cctcaggaat tttctatgga gggagacagc cgtggtctgc
     2341 aaattggcac tttagacaag ggtatccaaa gggtcatggt tgccctcgat attcgtgaag
     2401 agacggtggc agaggccatt gaaaagggtg tggacttgat tatcgtcaag cacgcgccta
     2461 tctttcgtcc tatcaaggac ttgcttgcca gccgtccgca aaatcagatt tacatcgacc
     2521 tgattaagca tgatatcgca gtttatgtca gccataccaa tattgatatc gttgaaaatg
     2581 ggctcaatga ctggttttgt cagatgctag gaatcgagga gacgacttat ctgcaggaaa
     2641 caggtccaga acgtggaatt ggacgtattg ggaatattca gcctcagaca ttttgggaat
     2701 tggcccaaca ggtcaagcaa gtctttgacc tagatagcct tcgaatggtg cattatcaag
     2761 agaatgattt gcagaagcct atttcaagag tagcaatttg tggtggaagc gggcagtctt
     2821 tctataagga tgctttggca aagggggcag atgtctatat cactggcgac atctactacc
     2881 atactgctca ggatatgctg tctgatggct tgttagcatt ggatccaggt cactatatcg
     2941 aagtgctttt tgtggaaaaa atcgcagcac tccttactca atggaaagag aaaaaaggct
     3001 gggaactaga gattttacct agtcaagtat cgaccaatcc tttccgtcat atctagttag
     3061 aaagtgaaga caatgaaaaa agttgccatt attggagcag gaattgtggg agcaacagct
     3121 gcctactacc tctcgagaga aagtgaccta gaggtgaccg tttttgacca tgagcaaggt
     3181 caagccacca aggccgcagc aggaattatc agtccttggt tttccaaacg ccgtaataaa
     3241 gcctggtaca agatggcgcg cttgggggct gatttttatg tggatttatt agctgattta
     3301 gagaaatcag gacaaaaaat cgacttttac cagcgttcgg gagtctttct cttgaaaaag
     3361 gatgaatcca agttggaaga actttatcaa ctggccctcc agcgcagaga agaatctccc
     3421 ttgatagggc aattagccat tctggaccaa gcttcagcta atgaattatt ccctggtttg
     3481 cagggatttg accgcctgct ctatgcttct ggtggagcga gagtagatgg ccaactcttg
     3541 gtaactcgtc tgctagaagc cagtcatgtc aagctggtca aagaaaaagt gactctgaca
     3601 ctcttatcat caggctacca gattggcgaa gaggtgtttg atcaggttat tttggcaacg
     3661 ggagcttggt tgggggacat gttagagcct ttaggttatg aagtggatgt ccgtcctcaa
     3721 aaaggacaac tacgagatta tcagcttgcc caagacatgg aagcttaccc tgttgtcatg
     3781 ccagaagggg agtgggattt gattcccttt gcaggtggga aattatcctt gggcgctacc
     3841 catgaaaatg acatgggatt tgatttgacg gtagatgaac ccttgctcca acaaatggag
     3901 gaggaggcct tgcctcatta cccagtttta gctagagcga cttcaagggc tgagcgtgtg
     3961 ggaatccgtg cctataccag tgatttctct cccttctttg ggcaggtgcc agaattggca
     4021 ggtgtctatg cagccagtgg actaggttca tcaggcctca caactggtcc tatcattggt
     4081 taccatctag cccaactgat ccaagacaag gagttgacct tggaccctct aaattaccca
     4141 attgaaaact atgtcaaacg agtaaaaagc gaataatact caatgaaaat caaagaacaa
     4201 actaggtaac tagccgcggg ttactcaaag cactgctttg aggttgtgga tagaactgac
     4261 gaagtcagct caaaacactg ttttgaggtt gtagatagaa ctgacgaagt cagtaaccat
     4321 acccatggca aggcgacgtt gacgtggttt gaattggatt ttcgaagagt ataagcattt
     4381 tactgaaatt ttatcaggta gtttaggatg gcaaatgaca ttccctgtca aaaatggtaa
     4441 aataagaaaa aataatctga gaatcgagga aaaaagatgc aagaaaagat tttggtaact
     4501 ggtggtgccg gttttattgg aacccacact gttattgaat tgatccaagc aggccatcaa
     4561 gttgttgtgg tggataatct tgtcaatagc aatcgtaaga gtttagaagt tgttgaaaga
     4621 atcacaggag ttgagattcc tttctatgag gcagatattc gtgacacaga taccctcaga
     4681 gatattttca agcaggaaga accgactggt gtcattcact ttgctggttt gaaggctgtt
     4741 ggcgaatcaa cacgtatccc tcttgcctac tatgacaaca atatcgctgg aactgtcagc
     4801 cttttgaaag caatggaaga aaacaactgt aaaaacatca tcttcagttc ttctgcgaca
     4861 gtttacgggg atccgcacac agtgcccatc ttggaagatt tcccactttc agtgaccaac
     4921 ccatacggtc gtactaagct catgctagag gaaattttga ctgatattta caaagcagac
     4981 tcagaatgga atgttgtctt gcttcgttac tttaacccaa tcggagccca tgagagtggt
     5041 gatttgggag aaaatccaaa cggtattcca aacaatctct tgccatatgt gactcaagta
     5101 gccgttggaa aattagagca agtgcaagtg tttggagacg attacgatac ggaagatgga
     5161 acaggtgttc gtgactatat ccacgttgtc gatttggcta agggtcacgt tgcagctttg
     5221 aaaaaaatcc aaaaaggttc aggactaaac gtttataacc ttggaactgg taaaggttac
     5281 tcagttcttg aaattatcca aaacatggaa aaagcggtgg gacgtcctat tccttaccgc
     5341 atcgtagaac gtcgcccagg tgatatcgct gcctgctact cagacccagc aaaagctaaa
     5401 gcagaactcg gttgggaagc agaactcgac atcacccaaa tgtgtgaaga cgcatggcgt
     5461 tggcaaagca agcatccaaa tggatttgaa gactaagatg atgatttcaa tcatcgtccc
     5521 ttgtttaaac gaagaggaag tacttcctct tttttatcag gctctggaag ctttacttcc
     5581 agatttggaa acagaaatcg agtatgtctt tgtcgatgat ggatcaagtg atgggacctt
     5641 ggaactctta aaggcctatc gggagcaaaa tccggcagtc cattatattt ctttctctcg
     5701 aaattttggc aaagaagcag ccctttatgc aggcttgcaa tatgcgacag gagatttggt
     5761 ggtggtgatg gatgcagacc tccaagatcc tcctagtatg ttgtttgaga tgaaaaatgt
     5821 actagacaaa aatgtagact tggactgcgt tgggacacgg agaactagtc gggagggaga
     5881 acccttcttt cgcagtttct gtgctgttct cttttatcgc ctcatgcaaa aaatcagccc
     5941 agtagctctg ccgtcgggtg tccgtgattt tcgtatgatg agaaggtctg tagtcgatgc
     6001 cattttaagc ttgaccgagt ccaatcgttt ttcaaagggg ctcttcgcct gggtgggttt
     6061 caaaacctac tatttggact atccaaatgt cgaaaggcag gctggcaaga caagttggag
     6121 ttttaggcaa ctctttttct actccattga agggattatt aatttttcag attttccctt
     6181 gattatagcc tttgtagctg gtctcctatc ttgttttctt tctctgctga tgaccttttt
     6241 tgttgtggtt cggaccctca ttttgggcaa tccgacatct ggttggacct ctctgatggc
     6301 tgttattctc tttcttggag gcattcaact cttgaccatt gggattctcg gcaagtatat
     6361 cagtaagatt tatttagaga ctaaaaaaag accactttat cttatcaaag aaaaaagtga
     6421 ccttcctgat tttacagaaa aaaataaagt gaaaagacta taattttaca tggaaatgtg
     6481 ctaaactaga gggagtggat tatcgccctt gatttaggct tcctggtttc caatcagggc
     6541 gcgagttggg caatttttaa cagcctctaa gacatcctga ctaggagaaa tttctttttc
     6601 cagttggtca gggtcatcgt aaaaacgcac gattccatta tcgtggtaat caaataaatc
     6661 agaataagtt tggcaaagcc cacaggcgat acatcgttca ggtataagtg tgattttcat
     6721 gttcatattt atattgtaat aagaaaagta aaaaaaaaca aggagtaagg tatggcaaaa
     6781 gaaccgtggc aagaagatat ctatgatcaa gaagaatcaa gagcagagcg tcggcatcga
     6841 aaccacggag gggctgatag gatggctaat cgtattttga cgatcctagc tagtattttc
     6901 tttgtaattg tggtggtgat ggtcatcgtt ctcatctatc tatcatcggg gggaagtaat
     6961 cgcacagcag ccttaaaaga ctttcatgat tctgatgcaa gtgtagtaca aatctcatct
     7021 tcaagcagtt ctcagcctga gcagagttca gagccagaat ctacttctag tagttcagaa
     7081 gaagctgcta atcctgaagg aacgattaaa gttctcgcag gagaagggga agcagctatt
     7141 gccgctcgtg caggaatctc cattgctcag ttagaggcct tgaatcctgg gcacatggct
     7201 acaggatctt ggtttgctaa tccaggtgat gttataaaaa taaaatagga gccgatatga
     7261 aaacaattca aattgctatt gatggtcctg cttccagcgg taagagtacg gtcgcaaaga
     7321 ttattgctaa ggattttgga ttcacctacc ttgatacagg agctatgtat cgtgcagcga
     7381 cctatatggc tcttaagaac caattaggag ttgaagaagt cgaagccctt ctagccttgt
     7441 tggaccagca tccaatcagc tttggacgtt cagaaactgg agaccagctt gtttttgtag
     7501 gagatgtgga tattacccat cctatccgtg aaaatgaagt gaccaatcat gtttctgcta
     7561 ttgcagcaat tcctgaagtg cgtgagaaac tggtttctct ccaacaagaa attgcccagc
     7621 aaggcgggat tgtcatggat ggtcgcgata ttggaactgt tgtattgcca caagcagaat
     7681 tgaaaatttt cctagtagct tctgttgatg agagagcaga gcgtcgttac aaggaaaata
     7741 ttgccaaggg aattgaaaca gaccttgaaa ccctaaaaaa ggaaattgct gcgcgtgact
     7801 acaaggatag tcatcgtgag acttctcctc tcaaacaagc agaggatgct gtctaccttg
     7861 atacaactgg tttgaacatt caagaagtag ttgaaaaaat caaagcagaa gctgaaaaaa
     7921 gaatgtaaat gtagaaaagc cgatagattg atatcggttt tttctaaatt tgtcaaaatg
     7981 ctgattttag ggtataatag ttgctgttcg agaaattttg attttcaaag aatagtgaat
     8041 gatgataagg agaaatcatg aacaatttac caaattgtcc aaaatgtaac tcagagtatg
     8101 tctacgaaga cggtgcccta ctggtttgcc cagagtgtgc tcatgagtgg aatcctgctg
     8161 aagttgcaga agtagaagaa ggtcttgtcg ctatcgatgc caacggaaat aaattggctg
     8221 atggtgatac agtaactctc atcaaggact tgaaagtaaa aggtgcgcca aaagatttga
     8281 aacaagggac gcgcgtgaaa aatatccaca tcgtagaagg cgaccacaat atcgactgta
     8341 agattgatgg ttttggtgcc atgaaactta aatcagagtt tgtgaggaag atttaagcat
     8401 gtcaaaacac tataaacttg tattttatag ccgtatcttc ttgtttctag cggcttttac
     8461 gggagtttat cttgaaatta ctaagcatgg tggttttggg atgcttctct attacacggt
     8521 tctgtccaac ctcttggtaa ctatttttac cctttatctt ctaaaggtta tgagccgtgt
     8581 aggtgaaaat tggcaaagac caagtctctt gcgcttaaaa ggtggggtca ccatgagtat
     8641 catgattacc tgtgtgattt accatttcct cttggcgccc attgcgacta atttctatac
     8701 cctagaaaat ttcctttgcc actatatcgt tcccatctgg tttttagcgg ataccctctt
     8761 ttttgacaaa cagggtcaat acaagatttg ggatccagca gtgtggacca ttttaccctt
     8821 tctgtatatg atgtttgctc tttttaatgg cttggttcta aaactcaata ttccaaatgc
     8881 caaggataat cctttccctt acttcttttt gaatgtgaac aagggttgga atgttgtgtt
     8941 taagtggtgt ctgattatct ttgttgccta tatggtagca ggatttattt tctactttat
     9001 caagcaaatc aagagaaagt catcctaata ctcttcgaaa atctcttcaa accacgtcag
     9061 tttccatctg caacctcaaa acactgtttt gaacaacctg cggctagctt cctagtttgc
     9121 tctttgattt tcattgagta taagatacaa gggattcatt ttcgaattta agaaggagtc
     9181 tacaaaccaa tgaggcttct tcttttcttg cttatttgat aactatcaaa aaagtagaag
     9241 attaagagaa atatagaaag agatttcatg aaacaatttt tagaacgggc cagcattttg
     9301 gctctctccc tcgttttgat tacctccttt tccatttcga gtgccctacc agccatgttt
     9361 gactattatc agggttattc taaggaacaa attgagctct tggtgagctt gccttccttt
     9421 ggaatcatga tgatgttact gctaaatggt ttcttagaaa aaatatttcc tgagcgctta
     9481 cagattagtt tgggcttgct gattttatca ttgagcggta cagctccctt ctggtaccaa
     9541 gcctatccct ttgtctttgg agcacggctt ctctttggtt tgggtcttgg gatgatcaat
     9601 gccaaggcca tttctattat cagtgaacgc taccaaggaa aaaggcgaat tcagatgtta
     9661 gggctacgcg cttctgcaga ggtcgttgga gcttctctca ttaccttggc cgtcggtcag
     9721 ttgttggcct ttggttggac agctatcttt ctagcctata gtgctggatt tttggtgctg
     9781 cccctttatc tgctctttgt cccttatgga aaatcaaaga aagaagtcaa gaaaagagcg
     9841 aaggaagcaa gtcgtttaac tcgagaaatg aaaggcttga tttttacctt agctatcgaa
     9901 gcggcagttg tagtttgtac caatacagct attaccatcc gtattccaag tttgatggtg
     9961 gaaagaggat tgggggatgc ccagttatct agttttgttc ttagtatcat gcagttgatc
    10021 gggattgtgg ctggggtgag tttttctttc ttgatttcta tctttaaaga gaaactgctc
    10081 ctctggtctg gtattacctt tggcttgggg caaattgtga ttgccttgtc ttcatccttg
    10141 tgggtggtag tagcaggaag tgttctggct ggatttgcct atagtgtagt cttgacgacg
    10201 gtctttcaac ttgtctctga acgaattcca gctaaactcc tcaatcaagc aacttcattt
    10261 gctgtattag gctgtagttt cggagccttt acgaccccat ttgttctagg tgcaattggc
    10321 ttactaactc acaatgggat gttggtcttt agtatcttag gaggttggtt gattgtaatc
    10381 tctatctttg tcatgtacct acttcagaag agagcttagg attgattcct aagtttttct
    10441 tttgatactc aatgaaaatc aaagagcaaa ctatagttga ttgagtttgg aatagtacgc
    10501 tgtagctgct aaaatatttc tagaaattaa tttgactttc ctaatagagt tattcatatc
    10561 ttatttcaat ctactataga aagctagccg caggttgctc aaaatagtgt tttgaggttg
    10621 cagatggaaa ctgacgtggt ttgaagagat tttcgaagag tatgagattt ttatacccat
    10681 acaatatttg ttttaggtcc ttgtttacag actaatttct tgataaaata gaggttatga
    10741 cgagatataa agcaactatt tcctatgatg gttatgcctt tgctggcttt cagcgccagt
    10801 ctcatgcgcg tagcgttcag gaagaaattg aaaaaacctt gaccagatta aataaagggc
    10861 aagccattac tgttcacggt gctggtagga cagatagtgg ggttcatgcc ctgggacagg
    10921 tcattcattt tgacctgcct tatcagattg atgaggagaa actccgtttt gccttggata
    10981 cccagtctcc tgaagatatt gatgtgattt cgattgagct tgtggcagat gattttcatt
    11041 gccgttatgc caagcatagc aagacctatg agtttattgt ggatagagga cgtcccaaaa
    11101 atccgatgcg ccgtcactat gccacccact ttccctaccc actcgatgtg gaacgaatgc
    11161 agattgcaat caaaaagcta gagggaaccc atgattttac cggttttaca gcctctggga
    11221 ctagtgtaga ggataaggtt cgcaccatca cagaagctag tttaatagtc gatgagacag
    11281 gacaattttt gacctttacc ttttccggaa atggtttctt gtataaacag attcgcaata
    11341 tggtggggac tcttctcaaa atcggtaaca accgcatgcc agtagagcag attgatctca
    11401 tcttggagaa gaaggacagg caacttgcag gtcccactgc agcaccaaat ggtttgtatt
    11461 taaaggagat tcgttatgaa gaataatcgt attttagcac tttctggaaa tgatattttt
    11521 agtggtggtg gactgtcagc tgatttggct acctatacct tgaacggctt gcatgggttt
    11581 gtagcagtga cttgtttgac agccttgaca gaaaaaggat ttgaagtctt tccaactgat
    11641 gataccattt ttcaacatga attagatagc ttgcgtgatg tggaatttgg gggaattaag
    11701 attggtcttc tccctactgt cagtgtggct gagaaggcct tggactttat caaacaacgc
    11761 ccaggagtac ctgtggtgtt ggatcctgtc ttggtctgca aggaaacgca tgatgtagct
    11821 gtcagtgagc tctgccaaga gttgattcgc tttttccctt atgtcagtgt gattacgcct
    11881 aatctcccag aagcagaatt attatccggt caggaaatta aaaccttgga agacatgaaa
    11941 actgcagcgc agaaattgca tgatttagga gcgccagcag tcattatcaa gggaggcaat
    12001 cgtcttagtc aggacaaggc tgtggatgtc ttttatgatg gacagacctt tactatccta
    12061 gaaaatccag ttatccaagg ccaaaatgct ggtgcaggtt gtacctttgc ctctagcatt
    12121 gccagtcact tgattaaagg tgataaactt ttgccagcag tagaaagctc taaggctttc
    12181 gtttatcgtg ctattgcaca agcagatcag tatggagtaa gacaatatga agcaaacaaa
    12241 aacaactaaa atcgcccttg tatccctatt aaccgccctt tctgtggttc taggttattt
    12301 cttaaaaatc ccaacaccta caggaattct aactctttta gatgctggtg tcttctttgc
    12361 ggccttttac tttggtagtc gtgaaggagc ggtagtcgga ggactagcaa gtttcttgat
    12421 tgacctccta tcaggctacc ctcagtggat gttctttagc ttggtcaacc atggcttgca
    12481 gggatttttc gcaggattta aaggaaaaag tcagtggtta ggccttattt tagcaactat
    12541 tgccatggta ggaggctacg ccttgggttc tactttgatg aatggctggg cagcagccct
    12601 cccagaaatt ctaccaaatt tcatgcaaaa tatggtaggg atgattgtag gatttattct
    12661 tagtcaaagt atcaagaaga ttaagtaata ctcttcgaaa atctcttcaa accacgtcag
    12721 tttccatctg caacctcaaa acactgtttt gagcagcctg cggctagctt tctagtctga
    12781 ttttcattga gcataaagag gttgagtaaa aagtctttca aataagaaaa acgcatagta
    12841 tcaggtgttg aatgaactgc cccccaaaag ttagacagaa aaaatctaac ttttgg
//
LOCUS       DAVLVZ010000050        12772 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000050 DAVLVZ010000000
VERSION     DAVLVZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.050"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1371..1655
                     /locus_tag="VYN77_001513"
     CDS             1371..1655
                     /locus_tag="VYN77_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558958.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            1902..3211
                     /locus_tag="VYN77_001514"
                     /pseudo
     CDS             1902..3211
                     /locus_tag="VYN77_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3284..4135)
                     /gene="srtC-3"
                     /locus_tag="VYN77_001515"
     CDS             complement(3284..4135)
                     /gene="srtC-3"
                     /locus_tag="VYN77_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
                     /protein_id="HEV1558959.1"
                     /translation="MSRTKLRALLGYLLMLVACLIPIYCFGQMVLQSLGQVKGHATFV
                     KSMTTEMYQEQQNHSLAYNQRLASQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLGMGLAHVDGTPLPLDGTGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
     gene            complement(4122..5015)
                     /gene="srtC-2"
                     /locus_tag="VYN77_001516"
     CDS             complement(4122..5015)
                     /gene="srtC-2"
                     /locus_tag="VYN77_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEV1558960.1"
                     /translation="MDNSRRSRKKGTKKKKHPLILLLIFLVGFAVAIYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTEQEKKKGVSEYAN
                     MLKVHERIGYVEIPAIDQEIPMYVGTSEDILQKGAGLLEGASLPVGGENTHTVITAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGEDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            complement(5002..5904)
                     /gene="srtC-1"
                     /locus_tag="VYN77_001517"
     CDS             complement(5002..5904)
                     /gene="srtC-1"
                     /locus_tag="VYN77_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEV1558961.1"
                     /translation="MVKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPVIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     KQPEKALKALKAARKEVKVEDGQQ"
     gene            complement(6242..7423)
                     /gene="rrgC"
                     /locus_tag="VYN77_001518"
     CDS             complement(6242..7423)
                     /gene="rrgC"
                     /locus_tag="VYN77_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEV1558962.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDNRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKADTVTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDGSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIVVTNLPLGTY
                     RFKEVEPLAGYTVTTMDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEENGHYTPVLQNGKEVVVASGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKPN
                     N"
     gene            complement(7470..9434)
                     /locus_tag="VYN77_001519"
     CDS             complement(7470..9434)
                     /locus_tag="VYN77_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEV1558963.1"
                     /translation="MKSINKFLTILAALLLTVSSLFSAATVFAAEQKTKTLTVHKLLM
                     TDQELDAWNSDAITTAGYDGSQNFEQFKQLQGVPQGVTEISGVAFELQSYTGPQGKEQ
                     ENLTNDAVWTAVNKGVTTETGVKFDTEVLQGTYRLVEVRKESTYVGPNGKVLTGMKAV
                     PALIILPLVNQNGVVENAHVYPKNSEDKPTATKTFDTAAGFVDPGEKGLAIGTKVPYI
                     VTTTIPKNSTLATAFWSDEMTEGLDYNGDVVVNYNGQPLDNSHYTLEAGHNGFILKLN
                     EKGLEAINGKDAEATITLKYTATLNALAVADVPEANDVTFHYGNNPGHGNTPKPNKPK
                     NGELTITKTWADAKDAPIAGVEVTFDLVNAQTGEVVKVPGHETGIVLNQTNNWTFTAT
                     GLDNNTEYKFVERTIKGYSADYQTITETGKIAVKNWKDENPEPINPEEPRVKTYGKKF
                     VKVDQKDERLKEAQFVVKNEQGKYLALKSAAQQAVNEKAAAEAKQALDAAIAAYTNAA
                     DKNAAQAVVDAAQKTYNDNYRAARFGYVEVERKEDALVLTSNTDGQFQISGLAAGSYT
                     LEETKAPEGFAKLGDVKFEVGAGSWNQGDFNYLKDVQKNDATKVVNKKITIPQTGGIG
                     TIIFAVAGAVIMGIAVYAYVKNNKDEDQLA"
     gene            complement(9658..12318)
                     /gene="rrgA"
                     /locus_tag="VYN77_001520"
     CDS             complement(9658..12318)
                     /gene="rrgA"
                     /locus_tag="VYN77_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HEV1558964.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYEQKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDPENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAAGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GDPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVLYDTTEKRIRVTGLYLGTDEKVTLTYNVRLNDEFVSN
                     KFYDTNGRTTLHPKEVEQNTVRDFPIPKIRDVRKYPEITISKEKKLGDIEFIKVNKND
                     KKPLRDAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
BASE COUNT         3829 a         2847 c         2035 g         4061 t
ORIGIN      
        1 gacaatatga aaacaagacg acttccatat aggaaaccgc cttctcgcta tgttgagtga
       61 tttatattaa aataactttt cttctagctg cattttatta ttataaaaac attcatcata
      121 acccccagaa cttaaataac aatttttatt caagatacat actcctagaa taaactttat
      181 atgaaattct catttttgtt tttacaattc tccttagtta aatcttgttt aatatatgtt
      241 ttacatatag tatttagcgc cacatagtac tgaactctct ccaaaaacgg ttattcctct
      301 ttgaataggg cgttatcaca agaaaagcat ctccacgttt caacttcata tggctcaaaa
      361 acaatcaatt gatgctaaaa cctgtaccta gatgtttcgg ttcataaaac catgaaactg
      421 taaaagtgga tgaaattgat agcgatagtc aaatcaagag gcatcataac tctaaaaagt
      481 cacaatatat aagttcatcc tcggaaaaat atcattctaa ttgttgaaat gcctacatga
      541 aaagaaacgt caaatgctca tgaaacaacg aatacaggta tcaaaactat gacaaaacaa
      601 atccctaaat ttactaaaga cactgctcaa ctttacacct gtaaatggtt gttgtataat
      661 aaagttacaa agatgtacga ccacactgtt gtaaatcata gtgttcgcga atatattact
      721 gatagcattt ctacaaatac aagtaaagag agcggatgag attcaaacga aatatgtcag
      781 tgctttggca ttcctagcct tcatatcatt taaagaattc tatagacaaa attttttcca
      841 atacagacac tcgtaacaac tgcttcattt ttctaccaac atatttagga acaggataag
      901 atacaagagt attaatccat agctcagttc tataccaatc taagacaaat aagctaaaaa
      961 aacgattgat aataagcaaa tagattccaa attttctcta tctgctcatt ttaataaaca
     1021 atactagtgt aactatcctt ccagtcagaa acttgtcaaa tcacaccgaa aactcttcta
     1081 aaatttatct cgttaggcaa tcaagcaaaa actcgacgat agtacaaaca ttatcataca
     1141 gaattgactg cctaaattat atactttagt aaggttttcg gataagaaaa aaggttcatt
     1201 ttacatttct aaacattctt ttctaagatg aaaaacagaa tttttcgatt gtgatttaaa
     1261 gcaacaagaa gattttcagt atcatcctat agatacgagc taattaagaa aaactacatt
     1321 tttgaatata aactacaata atataaacta aattttatag gaggaagaca atggattggt
     1381 acgattatat gatacaggca tccaaacaat cacaattcaa cgcaagccat tggtttcgct
     1441 atttgcgaaa agttattttt gaagactatt cttatttaac aaaccaagat gtagaaaagt
     1501 tgctagactc caaagaacta acccgttttc aaaaaattag cttgaagtat gcctttcaag
     1561 agcatactcc aactcataaa tatgtgattt cattaaataa acctgctaag ttaaccaatg
     1621 ttcaaaaatt gatggagaaa tacaaacatg gataaaatga aaccggtctt ccaagcccta
     1681 aataagaaat taattcagga aaatctgact ttaacaatta tggttatgtc ttagaatatc
     1741 atggtttacg tgccacacaa gatgttgatg cttttatggc tctataatat ttgtagtggg
     1801 taaatcccct atggatatta tggagcctat ttttgtgtag aaaaaaagtc ccatatgacc
     1861 tataatgaaa agcgacaaaa caactcatta gaaagaatca tatggaacaa ttacatttta
     1921 tcacaaaatt actagacatt aaagacccta atatccagat tttagacatc atcaataagg
     1981 atacacacaa ggaaatcatc gccaaactgg actacgacgc cccatcttgc cctgagtgcg
     2041 gaaaccaatt gaagaaatat gactttcaaa aaccgtctaa gatcccttac ctcgaaacaa
     2101 ctggtatgcc ttctagaatt ctccttagaa aacgccgttt caagtgctat cactgttcaa
     2161 aaatgatggt cgctgaaact tctatcgtca agaagaatca tcaaattcct cgtattatca
     2221 accaaaaaat tgcgcaaaag ttgattgaga agatttctat gaccgatatt gctcatcagc
     2281 tggccatttc aacttcaact gtcattcgca agctcaatga ttctcacttt gagcatgatt
     2341 tttcgcgtct tcctgagatt atgtcctggg acgttgaaac agtccgggga gtgactgttt
     2401 caatcgggag atggagatga gctttattgc gcaagatttt aacaatctca atatcatcac
     2461 tgttcttgaa ggtagaacac aagctgtcat ccgagatcac tttcttaaat atgatagagc
     2521 cgtccgatgt cgcgtcaaaa tcattactat ggatatgttt agcccttact atgacttggc
     2581 taaacagctt cgctttcaaa tttctaggct caggctgaaa cagtctccta atgctaaaat
     2641 cgttcttgac tgctttcata ttgtacaaca tcttagccgt gctatgagtc gtgtgcgtgt
     2701 ccaaatcatg aatcagtttc atcgaaaatc ccatgaatac aaggctatca agcgctactg
     2761 gaaactcatt caacaggata gctgtaaact gagtgataag cgattttatc gtcctatttt
     2821 tcgtatgcac ttaaccaata aagagatttt aaacaagctt ttgagctatt cggaagactt
     2881 gaaacaccac tatcaactct atcagctctt gctttttcac ttccagaata aggaaccaga
     2941 gaaatttttc ggacttattg aggacaatct taagcaggtt catcctattt ttcagactgt
     3001 ctttaaaacc ttcctcaaag ataaagaaaa gattatcaac gcccttcaac tacactattc
     3061 taatgccaaa ctggaagcga ccaataatct catcaaactt atcaagcgca atgcctttgg
     3121 ttttcgaaac tttgaaaact tcaaaaaacg gatttttatc gctctgaata tcaaaaaaga
     3181 aaggacaaaa tttgtccttt ctcgagctta gctttttttc aacccactac agttgacaaa
     3241 gagccggaaa aaggaacagc cttagctttc ctttcatttc tttttatttc cctcgtagta
     3301 aacgtgctag cttccacaaa acaaacagga ttcccagaaa tgccagtacc actagcccac
     3361 ggtacaacca ttgagaggtt gcaacacgcg atacagattg tccttctttc gtaaaagcaa
     3421 ccctcgcaac tgcagctgtt tgtggatctg atttttgata aacagcgact cgttcaaaat
     3481 tcactaataa gcgtttatta aaggtaggaa tcggatcgca ggttatcaag gtcatgatat
     3541 ttttagagct aaccgattct aatttttccc attccgacgg taaaataatc tctgtgtcca
     3601 tcatctgata ttctacaatt tcctggccat tatcataata aagagcatct ccaactttta
     3661 gctgatccaa atggcggaaa aagacatggc ttggctctgc acggtgccca gcaatcactg
     3721 agcgaatccc tgtaccatcc agaggcagcg gtgtaccatc cacatgagcc aagcccatcc
     3781 ctaaatgatg ataatctgct cccaaataaa ccggctccat gatttccaaa cttggaatag
     3841 acaagtaacc atagactgca tcagggtcgt cagacacttg gtaattgacc tcatatccct
     3901 ccgccaaaaa aggatctaca atgcgatttt gcgaagccaa gcgttgattg taggcgagag
     3961 aatggttctg ttgttcttgg tacatttcag ttgtcatgga tttcacaaat gtagcatgac
     4021 ctttcacctg tccaagagac tgcaacacca tctgtccaaa acaataaata ggaatcaaac
     4081 aggctaccaa catcaacaag tatcccaata aggctcgtag tttagtcctt gacatgacgc
     4141 ccctccaatt gcttttctag tcctttgaca atccgtcgat tacgatacac gcgatacagc
     4201 aagagaagga tgaccgccat cgctcctagt aataaccaca accagaattg cccacgctct
     4261 ctcaccgctc gattccgctc tgcaattggt gccgtatacg gaatccgctt cccacgtacc
     4321 aacagacgat gactgttaat catatacggt gtacaagtca acaaggtcgc ataatcttcc
     4381 ccatgttgaa tcaagacagg ctcaaagtca ttcggctcca ccgtcactat ctgatccact
     4441 tggtaggcca acacctgatc taaaacgtga agataaaaga tatccccttt tttcatctta
     4501 tccaattgac tgaacaattc tgccgttggc aatcctctgt gagcagtgat cactgtatgg
     4561 gtattttcac ctccaacagg cagcgaagcc ccttctaaca gccctgcccc tttctgaaga
     4621 atgtcctcac tcgttccgac atacatcgga atttcctgat caatcgcagg aatttccaca
     4681 tagccaatcc gctcatggac ctttagcata ttggcatatt ctgagacgcc tttctttttc
     4741 tcttgctctg taaaaggatc aagaatttca gatggtttca aggtcgcatt gaaggcttga
     4801 gccaagcgcc aacgctcctc aagttctgcc ttatccatct gggaaaccgt ctcatcaaac
     4861 tctttaataa cctcgtttga ctcaatacga taataataac gagacaccaa tggatatatc
     4921 gcaacggcga atcctactaa gaaaatcaga agaaggatca gcggatgttt cttctttttt
     4981 gtgccttttt ttcgtgaacg tctactgttg tccatcctcc accttcactt ccttccttgc
     5041 tgctttcagc gccttcaaag ccttttccgg ttgttttttc ttcttgcgca agcgtcgaat
     5101 aatccataaa agaatcacaa tcaaaccaac tgccacataa aacaggtagc gatagagatg
     5161 actgagtttg tttgctgcaa taaattcttc ctcaacctct gctacgtacg gtatccgatg
     5221 cccccgaacc aatagacgat gggtattgat catgtatggc gtacaagtca gcaaggtcac
     5281 ataatcatga cctggtacaa tcaataaatc atcaaagttc gtcggctcaa tcacctttac
     5341 ttgatccact tgataggcca tcacttcctt gatattgtgc acataaaact tatccccaac
     5401 tttaagtttg gtcaaatccg taaacatctt agctgttggc aaacctgtat gtgccgtaat
     5461 caccgcatgg gtcgaattgc ctccgatcgg cagagaagtt ccctctagat gcccagcccc
     5521 ttgctgcaat acctcttcag cagtaccagc ataaaccggc aaatccacgt caataacggg
     5581 gatttccaca tgccccatcc gctcatggat ttctaacata cgtgcatact ctgctcgccc
     5641 ttttttcttc atttcttccg accaaggatc gccactcact acattattca aagagtcatt
     5701 gaaggcttgt gccaatttca ttcgttcatc aatgtcagcc tcatccaacg ttgctttttc
     5761 cttatcaaag tcagcaattt gttgatttga ttccactcga taatacaagc gagacaccag
     5821 cggatacgcc attaccgcca ttccaatgaa aaagaccact cctaatagga gattatttcg
     5881 tttttgcttt tttgttttta ccatttttat cagcatccct ttatcttcaa actcatcatt
     5941 ttatcgcttt tcctaacaga caatcctatc tgctaagaga aacgagaaaa tctcatttct
     6001 ttctgattca ccatccattt taaatcagaa tcccagtcct gtccattcct aaaatgatta
     6061 ccagaccagt cctatcgttt tacaaacaat aacccacaat gaggctggca caatctgccc
     6121 agcctcatat ccgttattgt gattcatgcc acatcaccat gattcctgat taacatcacc
     6181 tcagagtact ctggtcttcc cttcagcctg ctatcttttt cataatgatg tacattgaat
     6241 atcagttatt tggttttttc gtaagataat aaccactacc aaacaacaaa atggcaacaa
     6301 gcatcaagat atacaaggtt tcttcccctg tatctggcac atcaatccgt ggtcgcttgt
     6361 tatttttaac cactgttacc agttccttac gagtatcttt cccgattgta aaggaaacag
     6421 gcgatgttaa ttgaacataa ccagttggag cttggagctc ccataaatag tatgtcccat
     6481 actctagacc ttccactcgg aaacgaccat ctttccctga tgccacaact acttccttac
     6541 cattttgaag aacaggagta tagtgtccgt tttcttcttt catgactttg aacattgccc
     6601 cttgaagaga ggtattggtc ctaccatcca ccttcataaa gtcaacattg ccacgtggta
     6661 atttctgatt gacaaccgta atcgtcacca gctgatgatc taccaactgg acatccgtat
     6721 ccatcgtcgt aacagtatag cctgccagtg gctccacctc cttgaaacga taggtcccaa
     6781 gaggaagatt tgtcacaaca atctctccat ttttatcagt atagagagtt ctacctactt
     6841 gaccagaaga actgtaacgg tattctccaa tcaagggaac ctctttttca gaaccatctc
     6901 ttgctactga taccaattta aagccgacac cctccaagcg attgtggtct tgatccacct
     6961 ttatcagctt cacctttgtt gtcaccgtat ctgctttttt cgctacaatg accaaaggct
     7021 ctaccgtctg atctgtcatt tcaaaaagaa attcagctgg ataagaaacc gcatccgtct
     7081 ggataataga gcgaacatag taaagaccat ttggaatatg agatacttca atctgattct
     7141 caaggaaggt catctcaaac gaagtctttt tgaaagaaga aagtttattc tcatcccacg
     7201 aatgcaagtc tctcacaatt tgcacccgat tatcatagga atacgaatca tccaacttcc
     7261 atacctgcaa ccgatgacca tcacgagatg gcaattgact aaccacctcc tgatagttct
     7321 ccaattgcaa gaccaacgtg tgatcttctt gcgcttggac tgcatgtgca ccccatacaa
     7381 gagaaaaaca cagagccata acaaagaaga tacgactaat cattttctgc attttctgca
     7441 ttgtcatcaa tggctccttt ctctcttact taagcaagtt gatcctcatc tttgttgttt
     7501 ttaacatatg cgtacactgc aatacccata atcacagccc ccgctacagc aaagataatt
     7561 gtaccaatac cacccgtttg agggatcgtg atttttttgt tgactacttt tgtagcgtcg
     7621 ttcttctgaa catcttttaa ataattgaaa tcaccttgat tccaagaacc tgctccaacc
     7681 tcaaacttca catctccaag ttttgcaaag ccttctggag cttttgtttc ttccaacgtg
     7741 tagcttccag cagcaagacc tgaaatttgg aattgaccat cagtgttaga agtaagaact
     7801 aacgcatctt cttttctctc tacttctaca tagccaaatc tagctgctct gtaattgtca
     7861 ttatatgttt tttgcgcagc atctactaca gcttgtgctg catttttatc tgcagcattt
     7921 gtataggctg ctatcgctgc atctagcgct tgtttcgctt ctgcggcagc tttctcattt
     7981 acagcttgtt gtgctgcaga tttgagtgca agatatttcc cttgctcatt cttcacaacg
     8041 aattgtgctt cttttaagcg ttcgtctttt tggtcaacct taacgaattt tttaccatat
     8101 gtttttacac gtggctcttc aggatttatt ggttctggat tttcatcttt ccagttttta
     8161 acagcaattt ttcctgtttc agtaattgtt tggtaatctg cagaatatcc cttaattgtc
     8221 cgttcaacaa atttatattc tgtattatta tcaagacctg tagcagtaaa tgtccaatta
     8281 tttgtttgat tcaatacaat acctgtttca tgtccaggta ctttaacgac ctcacctgtc
     8341 tgagcattta ccaaatcaaa agttacttct acacctgcta taggagcatc tttagcatca
     8401 gcccatgttt tagtaattgt aagttcaccg tttttaggtt tgtttggttt tggagtgtta
     8461 ccatgacctg ggttgtttcc ataatggaat gttacatcat tcgcttctgg cacatcagca
     8521 acagcaagag catttaaagt tgcagtatac ttcaacgtaa ttgttgcttc tgcatcttta
     8581 ccgttgattg cttccagacc tttttcattt aacttcaaga taaagccatt atgacctgct
     8641 tctaatgtgt aatgagaatt atcaagcggt tgaccattat aattaacaac tacatcacca
     8701 ttataatcta gaccttctgt catttcatct gaccagaaag ctgttgcaag agttgagttt
     8761 ttcggaatag ttgttgtaac aatatacggt accttagtgc caattgctaa acctttttca
     8821 cctggatcta cgaaacctgc tgcagtatca aatgttttcg ttgctgtagg tttgtcttca
     8881 gaattctttg gatagacatg tgcattttct acaacaccat tttggtttac aagcggcaga
     8941 ataattaaag caggaacagc tttcatacct gttaaaactt taccatttgg accgacataa
     9001 gtcgattctt tacgtacttc gacaagacga tatgtccctt gtaaaacttc agtatcaaat
     9061 ttaacacccg tttcagtcgt cacaccttta ttaaccgcag tccaaaccgc atcattcgtt
     9121 aaattttctt gttcttttcc ttgaggaccc gtataactct gtaactcgaa tgcaacacca
     9181 gagatttcgg ttactccttg tggaacacct tgaagttgtt tgaactgttc aaaattttgc
     9241 gaaccgtcat aacctgcagt agtaatcgca tcagaattcc aagcgtcaag ctcttgatct
     9301 gtcatcaata atttatgaac tgtaagtgtc ttagtttttt gttccgccgc aaaaactgtt
     9361 gcagctgaga acaggctact cactgtcagt aataaggcag caagtattgt taaaaatttg
     9421 ttgattgatt tcatgatttc tccttattca tatctcttaa aatcaactgt gagcaatgta
     9481 atgaattaca catgacaata ataatccttt ttctgtgcaa gttttcgaaa acatctcccc
     9541 tttcaatgat agtttactta cgaataaaac aggtttcacc aagtaaactt aaatctcttc
     9601 ttgagtgcta cttcttaaaa gtatcgttca gaacatagat attatcattt ctacacttta
     9661 cggatgtttc cgtgtgtata atagaactcc tcccatcatc atgcaaccta tcagatagaa
     9721 tggcaacatt ccgataccac cagttcgagg atattctctc tttggaggaa taggttcatt
     9781 ggtaatatag tgcttatcat tcgtaaactc gtaacccgct ggtatatctt gtggaacgat
     9841 tgaagtcaca tctctgactt ctccatttac tatttggaag gcaacgatag gcttattttg
     9901 aacgggttta taaccagctg gttcagaatt ttcaaataat cgatatttcc catctgacag
     9961 atttttaaag gtcaacttac catcttcacc tgttctcaca ttttgataag tgccattttg
    10021 atcaatagct ccataaatat ctggataatc cggatgttgt ttttgaagac taaagaccgc
    10081 atctctcagt ggttttttat catttttatt gaccttaata aactcaatgt caccaagttt
    10141 tttctctttt gaaattgtga tttctggata cttccgcaca tcacgaatct taggaatcgg
    10201 gaagtcgcgc actgtgttct gttctacttc cttaggatgt aaggttgttc gaccattggt
    10261 atcataaaat ttattgctta caaactcatc attcaaacga acattgtagg tcaacgtaac
    10321 tttttcatcc gttccaaggt acagacctgt tacacgaatc cttttctcag tcgtatcata
    10381 gagcactttt gcatttttta acaaaccacc atcattttgt ggaccaccta cagcttgtcc
    10441 attctccaag cgactaccat cgtttgcagt taaagtgtaa tctgctggat caaatcttcc
    10501 atctgtgccc aattgcaaat caattaactc acccatcgga tctgtaatcg taccattctc
    10561 aattgatttc ttttcagtta cgatggtgtg gaaataacga ttcaactgtt ccaatatttt
    10621 tgtcgtgtca gtaacattgg tatagttttc aggtttacta gaaatacttt gcataaaact
    10681 agtagccgtt gcttcatccg taccaggatc tccgttaata ccaataccta ccgtaaagac
    10741 atcataccca tcaggagcaa tattcccgtt atagtaccat cttgtagggt caccatgatt
    10801 ggtaatttta gcagtattag aattaaacgg ataagccaga atactctctc tccaattaag
    10861 ccaaatatat ccaccattaa ttggatcgcc tataactgca taaccagccg ccttcatttc
    10921 agaatatttt tcaggtttaa ctgggaaagc tgcaggagca cctttttcat aaactgtctt
    10981 atctgtaaac atctggtaac tttgcccatc tccgcgtaca attgtatgtt ctccactagt
    11041 tgcttgcgta ataaaatcac ttaatagtat tccatcttta ttaggagatt tactaaaaaa
    11101 tgcatttagt tgattttgat atgatggagc aaacgtagca tgattaaaat taatcggata
    11161 cgacatagtt gggacaccat ccgtaatatg gaaaatgact ttttgactat tttgtctcgc
    11221 ttgttgtgtc aaaatctcat ctgccttcat caaagctttc tgagtaaaag tggcaccgaa
    11281 ttggtacatc aatctatttc catcatggtc ttctgcctcg gtaggtacct tattttttaa
    11341 ttctacaatg tcattcttat cattagtcag ctttaaataa ctataatctt tggtattggt
    11401 tgtaaaactc gtctgatcat aattccaaaa aagagaatca ttcaatcgtt ttccgttttt
    11461 atctgctacc cctttttcta ctgtaaactc ggtcccatca aagatagtgg aagcataagt
    11521 cacaagcgct accctatttt ctggatcaga tgtaatttta tcaataagag aacgtgtcgc
    11581 ctcaccagct ctttccgcac gtcgagcatt cttgtttcga atgttactca tactatttga
    11641 gttatcgagc aagataacga catccagcgg cacagactta tctttttgtt catacactgt
    11701 tttcccacta accgtcaatt cgattccata ttggttatca tccaaattat tcacttgata
    11761 aattctcttt gaaagtgtac cttctggaat cacacgttca tatggattcg gattcaacgc
    11821 cttgtgctgt ccgttttttt ccgaaccatc taccttaata atctgataag gtgtttgaac
    11881 atctggataa gtccctgttt gtggatactg gtcagataga gcctcttctc gattttctac
    11941 ctgttcacct tggacagtcg tccgaccatt cttctcaact tcaacagtcc attgtttagt
    12001 agagggttta taaccaactg gaggttgggc ttctgtcaag gtgtatgtcc caggttttat
    12061 gtttgaaaat atcgcttctc ctgtttgcgc ctctgtcctt tgcgaaacag ttgtgccatc
    12121 cgtattgttt ttcaactcaa agacggcatc tcctagaagc gctcctcctt cgcctgtctc
    12181 cttaatcact acttttccta tcgctggact ggtttcaggc gtttctgcta aagcaactat
    12241 cgaagaaaaa gctgtcaact gagatataca gcacagtcct gcaactgcct tctgaaatat
    12301 ctttcttact tttttcatgt gtgtctccct gttaagcatt attcatagaa caagttaatc
    12361 cttctatgat tcataggtac tttcaatctg tatacacgaa gaaacggatt acttatgttc
    12421 tgataaaatt tatctcactg agttaatccg ttcactaaca aacgtagcaa aagcgtataa
    12481 aagtgcgtta tagctatctt ctcaacctgg caatgacaca cttgtataca atatagtata
    12541 gtattactat taactatcct agtataaatt aaaacaactt ccatcacaat agatattttt
    12601 atcacatatt tttttataga acgacaatat ttgataaaaa aatattttct aataaaaaaa
    12661 ataagccata aaactccacc aatactcatt actatcttta aaatgccttt tattacatgc
    12721 tgttttatca ataaagtcgg aaattccaca acttttttga caagacaatt ag
//
LOCUS       DAVLVZ010000051        12556 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000051 DAVLVZ010000000
VERSION     DAVLVZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.051"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..1076
                     /gene="dnaN"
                     /locus_tag="VYN77_001521"
     CDS             <1..1076
                     /gene="dnaN"
                     /locus_tag="VYN77_001521"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1558965.1"
                     /translation="ISSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQKNEDA
                     GLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQY
                     PRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATD
                     SHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENIS
                     FYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDG
                     VVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVR
                     PFTLVPADTDEDFMQLITPVRTN"
     gene            1141..1335
                     /locus_tag="VYN77_001522"
     CDS             1141..1335
                     /locus_tag="VYN77_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1558966.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1419..2534
                     /gene="ychF"
                     /locus_tag="VYN77_001523"
     CDS             1419..2534
                     /gene="ychF"
                     /locus_tag="VYN77_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1558967.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLIESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYESEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2605..3174
                     /locus_tag="VYN77_001524"
     CDS             2605..3174
                     /locus_tag="VYN77_001524"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1558968.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     KADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3175..6684
                     /gene="mfd"
                     /locus_tag="VYN77_001525"
     CDS             3175..6684
                     /gene="mfd"
                     /locus_tag="VYN77_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1558969.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADFRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            6752..7018
                     /locus_tag="VYN77_001526"
     CDS             6752..7018
                     /locus_tag="VYN77_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1558970.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7011..7379
                     /locus_tag="VYN77_001527"
     CDS             7011..7379
                     /locus_tag="VYN77_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1558971.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7499..8767
                     /locus_tag="VYN77_001528"
     CDS             7499..8767
                     /locus_tag="VYN77_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1558972.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8764..10041
                     /gene="tilS"
                     /locus_tag="VYN77_001529"
     CDS             8764..10041
                     /gene="tilS"
                     /locus_tag="VYN77_001529"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1558973.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPKLEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKTQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10045..10587
                     /gene="hpt"
                     /locus_tag="VYN77_001530"
     CDS             10045..10587
                     /gene="hpt"
                     /locus_tag="VYN77_001530"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1558974.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEVDYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10603..>12556
                     /gene="ftsH"
                     /locus_tag="VYN77_001531"
     CDS             10603..>12556
                     /gene="ftsH"
                     /locus_tag="VYN77_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1558975.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         4319 a         1891 c         2558 g         3788 t
ORIGIN      
        1 ctattagttc taaaaatgcc attcctattt tatcaacagt aaaaattgac gtgaccaatg
       61 aaggtattac tttaattggt tcaaatggtc aaatttcaat tgaaaatttt atttctcaaa
      121 aaaatgaaga tgctggtttg ttaattactt ctttaggttc gatccttctt gaagcttctt
      181 tctttatcaa tgtagtatct agtttacctg atgtaactct tgattttaaa gaaattgaac
      241 aaaatcaaat tgttttaacc agtggcaaat cagaaattac cctaaaagga aaagatagcg
      301 aacaatatcc acgaatccaa gaaatttcag caagcactcc tttaatactt gaaacaaaat
      361 tactcaagaa aattattaat gaaacagcct ttgctgcaag tacacaagag agtcgtccga
      421 ttttaacagg tgtccacttc gtattgagtc aacacaaaga gttaaaaaca gttgcaacag
      481 actctcatcg cctaagccag aaaaaattga ctcttgaaaa aaatagtgat gattttgatg
      541 tcgtaattcc tagccgttct ctacgcgaat tttcagcggt atttacagat gatatcgaaa
      601 ctgtagagat tttctttgcc aataatcaaa tcctctttag aagcgaaaat attagcttct
      661 atactcgtct cctagaagga aactatcctg atacagatcg cttgattcca acagacttta
      721 acactactat tacttttaat gtggtaaact tacgccagtc aatggagcgt gcccgtcttt
      781 tatcaagtgc gactcaaaat ggtactgtga aacttgaaat taaggatggg gttgttagcg
      841 cccatgttca ctctccagaa gttggtaaag taaacgaaga aatcgatact gatcaggtta
      901 ctggtgaaga tttgaccatt agtttcaacc caacttactt gattgattct cttaaagctt
      961 taaatagcga aaaggtgact attagcttta tctcagctgt tcgtccattt actcttgtgc
     1021 cagcagatac tgacgaagac ttcatgcagc tcattacacc agttcgtaca aattaagtga
     1081 aagaggttga gcctggctcg cctcttttat gatataatcg aaaaagaaaa ggagagtagt
     1141 atgtatcaag ttggaaattt tgttgagatg aaaaaatcac acgcttgtac aatcaagtcg
     1201 actggtaaaa aggctaatcg ttgggaaatt acacgtgtag gagcagatat caaaataaaa
     1261 tgcagtaatt gtgagcatgt tgtcatgatg gggcgatatg attttgagcg aaaaatgaat
     1321 aaaattattg actgagaacc cttagttaga gggttagcac tttatccctt tttttgttat
     1381 aatattaggg attgaaatga aaacggagaa tgagaaatat ggctttgaca gcaggtatcg
     1441 ttggtttgcc aaacgttggt aaatcaacac tatttaatgc aattacaaaa gcaggagcag
     1501 aggcagcaaa ctacccattt gcgactattg atccaaatgt tggaatggtg gaagttccag
     1561 atgaacgcct acaaaaacta actgaaatga taactcctaa aaagacagtt cccacaacat
     1621 ttgaatttac ggatattgca gggattgtaa aaggagcttc aaaaggagaa gggctaggga
     1681 ataaattctt ggccaatatt cgtgaagtag atgcgattgt tcacgtagtt cgtgcttttg
     1741 atgatgaaaa tgtgatgcgc gagcaaggac gtgaagacgc ctttgtggat ccacttgcag
     1801 atattgacac cattaaccta gaattgattc ttgcagactt agaatcagtc aataaacgat
     1861 atgcgcgtgt agaaaagatg gcacgtacgc aaaaagataa agaatcagta gcagaattca
     1921 atgttcttca aaagattaaa ccagtcctag aagacgggaa atcagctcgt accattgaat
     1981 ttacagatga ggaacaaaag gttgtcaaag gtcttttcct tttgacgact aaaccagttc
     2041 tttatgtagc taatgtggac gaggatgtgg tttcagaacc tgactctatc gactatgtca
     2101 aacaaattcg tgaatttgca gcgacagaaa atgctgaagt agtcgttatt tctgcgcgtg
     2161 ctgaggaaga aatttctgaa ttggatgatg aagataaaaa agagtttctt gaagccattg
     2221 gtttgataga atcaggtgta gataagttga cgcgtgcagc ttaccacttg cttggattgg
     2281 gaacttactt cacagctggt gaaaaagaag ttcgcgcttg gactttcaaa cgtggtatga
     2341 aggctcctca agcagctggt attatccact cagactttga aaaaggcttt attcgtgcag
     2401 taaccatgtc atatgaggat ctagtgaaat acgaatctga aaaggccgta aaagaagctg
     2461 gacgcttgcg tgaagaagga aaagaatata tcgttcaaga tggcgatatc atggaattcc
     2521 gctttaatgt ctaaaaatta ataaatggtg tcaattaggt tggaaaaaaa ttccaaccct
     2581 tttggctttt gaaaggaaaa ataaatgacc aaattacttg taggcttggg aaatccaggg
     2641 gataaatatt ttgaaacaaa acacaatgtt ggttttatgt tgattgatca actagcgaag
     2701 aaacagaatg tcacttttac acacgataag atatttaaag ctgacctagc atcctttttc
     2761 ctaaatggag aaaaaattta tctggttaaa ccaacgacct ttatgaatga aagtggaaaa
     2821 gcagttcatg ctttattaac ttactatggt ttggatattg acgatttact tatcatttac
     2881 gatgatcttg acatggaagt tgggaaaatt cgtttaagag caaaaggctc agcaggtggt
     2941 cataatggta tcaagtctat tattcaacat ataggaactc aggtctttaa ccgtgttaag
     3001 attggaattg gaagacctaa aaatggtatg tcagttgttc atcatgtttt gagtaagttt
     3061 gacagggatg attatatcgg tattttacag tctgttgaca aagttgacga ttctgtaaac
     3121 tactatttac aagagaaaaa ttttgagaaa acaatgcaga ggtataacgg ataaatggtg
     3181 accttattag atttattctc agaaaatgat cagattaaaa aatggcatca aaatttaact
     3241 gataagaaaa gacaactaat acttggttta tcaacatcta ctaaggctct tgcaattgca
     3301 agcagtttag aaaaagaaga taggattgtg ttattgacgt caacttatgg agaagcagaa
     3361 ggacttgtta gtgatcttat atctatcttg ggtgaggaac tcgtctatcc atttttggta
     3421 gatgatgctc ctatggtgga gtttttgatg tcttcacagg aaaaaattat ttcacgggtt
     3481 gaagccttgc gttttttgac tgattcatct aagaaaggga ttttagtttg taatatcgca
     3541 gcaagtcgat tgattttacc gtctcccaat gcattcaaag atagtattgt aaaaatctca
     3601 gttggtgaag aatatgatca acacgcgttt atccatcagt taaaggaaaa tggctatcga
     3661 aaagttactc aagtacaaac tcagggcgaa tttagtcttc gaggagatat tttagatatt
     3721 tttgaaatat cccagttaga accttgtcga attgagtttt ttggtgatga aattgatggt
     3781 atcaggtcat ttgaagtaga aacacaatta tcgaaagaaa ataagacaga actcactatc
     3841 tttccagcta gtgatatgct tttgagagaa aaggattatc aacgaggaca gtcagcttta
     3901 gaaaaacaaa tttcaaaaac tttatcacct attttgaaat catacctaga agaaattctt
     3961 tcaagttttc accaaaaaca aagtcatgca gactttcgga agtttttatc tttgtgctat
     4021 gataagacat ggactgtctt tgattatatt gaaaaagata ctccaatatt ctttgatgat
     4081 tatcaaaaat tgatgaatca gtatgaagtc tttgaaagag acttagcgca gtactttaca
     4141 gaagaattac agaatagtaa agcattttct gatatgcagt atttttctga tattgaacaa
     4201 atctataaaa aacaaagtcc agtgaccttt ttctctaatc ttcaaaaggg tttaggaaat
     4261 ctcaaatttg acaaaattta tcaattcaat caatatccta tgcaggaatt tttcaatcag
     4321 ttttcttttc taaaagaaga aattgaacga tataaaaaaa tggattacac cattattctg
     4381 cagtctagca attcaatggg aagtaaaaca ttggaggata tgttagagga atatcagatt
     4441 aaattggatt ctagagataa gacaaatatc tgtaaagaat ctgtaaactt aatagagggt
     4501 aatctcagac atggttttca ttttgtagat gaaaagattt tattgataac tgaacatgag
     4561 atttttcaaa agaaattaaa gcgtcgtttt cgaagacaac atgtttcaaa tgcagagaga
     4621 ttaaaagatt acaatgaact tgaaaaaggg gactatgttg tccatcatat ccatgggatt
     4681 ggtcaatatc taggaattga aaccattgaa atcaagggaa ttcatcgcga ttatgtcagt
     4741 gtccaatacc aaaatggtga tcaaatttct atccccgtgg aacagattca tctactgtcc
     4801 aaatatattt caagtgatgg taaagctcca aaactcaata aattaaatga cggtcatttt
     4861 aaaaaggcca agcaaaaggt taagaaccag gtagaggata tagctgatga tttaatcaaa
     4921 ctctactctg aacgtagtca gttgaagggt tttgctttct cagctgatga tgatgatcaa
     4981 gatgcctttg atgatgcttt cccttatgtt gaaacggatg atcaacttcg tagtattgag
     5041 gaaatcaaaa gggatatgca ggcttctcag ccaatggatc gacttttagt tggggatgtt
     5101 ggttttggaa agactgaagt tgctatgcgt gcagccttta aagcagtcaa tgatcacaaa
     5161 caggttgtca ttctagttcc gacgacggtt ttagcgcaac agcactatac gaattttaag
     5221 gaacgattcc aaaattttgc agttaatgtt gatgtgttga gtcgctttag aagtaaaaaa
     5281 gagcagactg caacacttga aaaattgaaa aacggtcaag tcgatatttt gattggaaca
     5341 catcgtgttt tgtcaaaaga tgttgtgttt gctgatttgg gcttgatgat tattgatgag
     5401 gaacagcgat ttggtgtcaa gcataaggaa actttgaaag aactgaagaa acaagtggat
     5461 gtcctaacct tgaccgctac gccaatccct cgtaccctcc atatgtctat gctgggaatc
     5521 agagatttgt ctgttattga aactccgccg actaatcgct atcctgttca gacctatgtt
     5581 ttggaaaaga atgatagtgt cattcgtgat gctgtcttgc gtgaaatgga gcgtggaggt
     5641 caagtttact atctttacaa caaagttgac acaattgttc agaaggtttc agaattacag
     5701 gagttgattc cggaggcttc gattggatat gttcatggtc gaatgagtga agtccagttg
     5761 gaaaatactc tattagactt tattgaggga caatacgata tcttggtgac gactactatt
     5821 attgagacag gggtggacat tccaaatgct aatactttat ttattgaaaa tgcggaccat
     5881 atgggcttgt caaccttata tcagttaaga ggaagagtcg gtcgtagtaa tcgtattgct
     5941 tatgcttatc tcatgtatcg tccagaaaaa tcaatcagtg aagtctctga aaagagatta
     6001 gaagcgatta aaggatttac agaattgggc tctggcttta agattgcaat gcgagatctt
     6061 tcgattcgtg gagcaggaaa tcttttagga aaatcccagt ctggtttcat tgattctgtt
     6121 ggttttgaat tgtattcgca gttattagag gaagctattg ctaaacgaaa cggtaatgct
     6181 aacgctaaca caagaaccaa agggaatgct gagttgattt tgcaaattga tgcctatctt
     6241 cctgatactt atatttctga tcaacgacat aagattgaaa tttacaagaa aattcgtcaa
     6301 attgacaacc gtgtcaatta tgaagagtta caagaggagt tgatagaccg ttttggagaa
     6361 tacccagatg tagtagccta tcttttagag attggtttgg tcaaatcata cttggacaag
     6421 gtctttgttc aacgtgtgga aagaaaagat aataaaatta caattcaatt tgaaaaagtc
     6481 actcaacgac tgtttttagc tcaagattat tttaaagctt tatccgtaac gaacttaaaa
     6541 gcaggcatcg ctgagaataa gggattaatg gagcttgtat ttgatgtcca aaataagaaa
     6601 gattatgaaa ttttagaagg tctgctgatt tttggagaaa gtttattaga gataaaagag
     6661 tttaaggaag aaaattccat ttgatatttt tcttctataa aatagataaa aatagataaa
     6721 aatggtacaa taataaattg aggtaataag gatgagatta gataaatatt taaaagtatc
     6781 gcgaattatc aagcgtcgta cagtcgcaaa ggaagtagca gataaaggta gaatcaaggt
     6841 taatggaatc ttggccaaaa gttcaacgga cttgaaagtt aatgaccaag ttgaaattcg
     6901 ctttggcaat aagttgctgc ttgtaaaagt actagagatg aaagatagta caaaaaaaga
     6961 agatgcagca ggaatgtatg aaattatcag tgaaacacgg gtagaagaaa atgtctaaaa
     7021 atattgtaca attgaataat tcttttattc aaaatgaata ccaacgtcgt cgctacctga
     7081 tgaaagaacg acaaaaacgg aatcgtttta tggggggggt attgattttg attatgctat
     7141 tatttatctt gccaactttt aatttggcgc agagttatca gcaattactc caaagacgtc
     7201 agcaattagc agacttgcaa actcagtatc aaactttgag tgatgaaaag gataaggaaa
     7261 cagcatttgc taccaagttg aaagatgaag attatgctgc taaatataca cgagcgaagt
     7321 actattattc taagtcgagg gaaaaagttt atacgattcc tgacttgctt caaaggtgat
     7381 aaaatggaaa atttattaga cgtaatagag caatttttga gtttgtcaga tgaaaagctg
     7441 gaagaattga ctgataaaaa tcaattattg cgtttacaag aagaaaagga aaggaagaat
     7501 gcgtaaattc ttaattattt tgttgctacc aagttttttg accatttcaa aagtcgttag
     7561 cacagaaaaa gaagtcgtct atacttcgaa agaaatttat tacctttcac aatctgactt
     7621 tggtatttat tttagagaaa aattaagttc tcccatggtt tatggagagg ttcctgttta
     7681 tgcgaatgaa gatttagtag tggaatctgg gaaattgact cccaaaacaa gttttcaaat
     7741 aaccgagtgg cgcttaaata aacaaggaat tccagtattt aagctatcaa atcatcaatt
     7801 tatagctgcg gacaaacgat ttttatatga tcaatcagag gtaactccaa caataaaaaa
     7861 agcatggtta gaatctgact ttaaactgta caatagtcct tatgatttaa aagaagtgaa
     7921 atcatcctta tcagcttatt cgcaagtatc aatcgacaag accatgtttg tagaaggaag
     7981 agaatttcta catattgatc aggctggatg ggtagctaaa gaatcaactt ctgaagaaga
     8041 taatcggatg agtaaagttc aagaaatgtt atctgaaaaa tatcagaaag attctttctc
     8101 tatttatgtt aagcaactga ctactggaaa agaagctggt atcaatcaag atgaaaagat
     8161 gtatgcagcc agcgttttga aactctccta tctctattat acgcaagaaa aaataaatga
     8221 gggtctttat cagttagata cgactgtaaa atacgtatct gcagtcaatg attttccagg
     8281 ttcttataaa ccagagggaa gtggtagtct tcctaaaaaa gaagataata aagaatattc
     8341 tttaaaggat ttaattacga aagtatcaaa agaatctgat aatgtagctc ataatctatt
     8401 gggatattac atttcaaacc aatctgatgc cacattcaaa tccaagatgt ctgccattat
     8461 gggagatgat tgggatccaa aagaaaaatt gatttcttct aagatggccg ggaagtttat
     8521 ggaagctatt tataatcaaa atggatttgt gctagagtct ttgactaaaa cagattttga
     8581 tagtcagcga attgccaaag gtgtttctgt taaagtagct cataaaattg gagatgcgga
     8641 tgaatttaag catgatacgg gtgttgtcta tgcagattct ccatttattc tttctatttt
     8701 cactaagaat tctgattatg atacgatttc taagatagcc aaggatgttt atgaggttct
     8761 aaaatgaggg aaccagattt tttaaatcat tttctcaaga agggatattt caaaaagcat
     8821 gctaaggcgg ttctagctct ttctggtgga ttagattcta tgtttctatt taaggtattg
     8881 tctacttatc aaaaagagtt agagattgaa ttgattctgg ctcatgtgaa tcataagcag
     8941 agaattgaat cagattggga agaaaaggaa ttaaggaagt tggctgctga agcagagctt
     9001 cctatttata tcagcaattt ttcaggagaa ttttcagaag cgcgtgcacg aaattttcgt
     9061 tatgattttt ttcaagaggt catgaaaaag acaggtgcga cagctttagt cactgcccac
     9121 catgctgatg atcaggtgga aacgattttg atgcgcttga ttcgaggaac tcgcttgcgc
     9181 tatctatcag gaattaagga gaagcaagta gtcggagaga tagaaatcat tcgtcccttc
     9241 ttgcattttc agaaaaaaga ctttccatca atttttcact ttgaagatac atcaaatcag
     9301 gagaatcatt attttcgaaa tcgtattcga aattcttact taccaaaatt ggaaaaagaa
     9361 aatcctcgat ttagggatgc aatcttaggc attggcaatg aaattttaga ttatgatttg
     9421 gcaatagctg aattatctaa caatattaat gtggaagatt tacagcagtt attttcttac
     9481 tctgagtcta cacaaagagt tttacttcaa acttatctga atcgttttcc agatttgaat
     9541 cttacaaaaa ctcagtttgc tgaagttcag cagattttaa aatctaaaag ccagtatcgt
     9601 catccgatta aaaatggcta tgaattgata aaagagtacc aacagtttca gatttgtaaa
     9661 atcagtccgc aggctgatga aaaggaagat gaacttgtgt tacactatca aaatcaggta
     9721 gcttatcaag gatatttatt ttcctttgga cttccattag aaggtgaatt aattcaacaa
     9781 atacctgttt cacgtgaaac atccatacac attcgtcatc gaaaaacagg agatgttttg
     9841 attcaaaatg ggcatagaaa aaaactcaga cgtttattta ttgatttgaa aatccctatg
     9901 gaaaagagaa actctgctct tattattgag caatttggtg aaattgtctc aattttggga
     9961 attgcgacca ataatttgag taaaaaaacg aaaaatgata taatgaacac tgtactttat
    10021 atagaaaaaa tagataggta aaaaatgtta gaaaacgata ttaaaaaagt cctcgtttca
    10081 cacgatgaaa ttacagaagc agctaaaaaa ctaggtgctc aattaactaa agactatgca
    10141 ggaaaaaatc caatcttagt tgggatttta aaaggatcta ttccttttat ggctgaattg
    10201 gtcaaacata ttgatacaca tattgaaatg gacttcatga tggtttctag ctaccatggt
    10261 ggaacagcaa gtagtggtgt tatcaatatt aaacaagatg tgactcaaga tatcaaagga
    10321 agacatgttc tatttgtaga agatatcatt gatacaggtc aaactttgaa gaatttgcga
    10381 gatatgttta aagaaagaga agcagcttct gttaaaattg caaccttgtt ggataaacca
    10441 gaaggacgtg ttgtagaaat tgaggtagac tatacctgct ttactatccc aaatgagttt
    10501 gtagtaggtt atggtttaga ctacaaagaa aattatcgta atcttcctta tattggagta
    10561 ttgaaagagg aagtgtattc aaattagaaa gaataatctt taatgaaaaa acaaaataat
    10621 ggtttaatta aaaatccttt tctatggtta ttatttatct ttttccttgt gacaggattc
    10681 cagtatttct attctgggaa taactcagga ggaagtcagc aaatcaacta tactgagttg
    10741 gtacaagaaa ttaccgatgg taatgtaaaa gaattaactt accaaccaaa tggtagtgtt
    10801 atcgaagttt ctggtgtcta taaaaatcct aaaacaagta aagaaggaac aggtattcag
    10861 tttttcacgc catctgttac taaggtagag aaatttacca gcactattct tcctgcagat
    10921 actaccgtat cagaattgca aaaacttgct actgaccata aagcagaagt aactgttaag
    10981 catgaaagtt caagtggtat atggattaat ctactcgtat ccattgtgcc atttggaatt
    11041 ctattcttct tcctattctc tatgatggga aatatgggag gaggcaatgg ccgtaatcca
    11101 atgagttttg gacgtagtaa ggctaaagca gcaaataaag aagatattaa agtaagattt
    11161 tcagatgttg ctggagctga ggaagaaaaa caagaactag ttgaagttgt tgagttctta
    11221 aaagatccaa aacgattcac aaaacttgga gcccgtattc cagcaggtgt tcttttggag
    11281 ggacctccgg ggacaggtaa gactttgctt gctaaggcag tcgctggaga agcaggtgtt
    11341 ccattcttta gtatctcagg ttctgacttt gtagaaatgt ttgtcggagt tggagctagt
    11401 cgtgttcgct ctctttttga ggatgccaaa aaagcagcac cagctatcat ctttatcgat
    11461 gaaattgatg ctgttggacg tcaacgtgga gttggtctcg gcggaggtaa tgacgaacgt
    11521 gaacaaacct tgaaccaact tttgattgag atggatggtt ttgagggaaa tgaagggatt
    11581 atcgtcatcg ctgcgacaaa ccgttcagat gtacttgatc ctgccctttt gcgtccagga
    11641 cgttttgata gaaaagtatt ggttggccgt cctgatgtta aaggtcgtga agcaatcttg
    11701 aaagttcacg ctaagaacaa gcctttagca gaagatgttg atttgaaatt agtggctcaa
    11761 caaactccag gctttgttgg tgctgattta gagaatgtct tgaatgaagc agctttagtt
    11821 gctgctcgtc gcaataaatc gataattgat gcttcagata ttgatgaagc agaagataga
    11881 gttattgctg gaccttctaa gaaagataag acagtttcac aaaaagaacg agaattggtt
    11941 gcttaccatg aggcaggaca taccattgtt ggtctagtct tgtcgaatgc tcgcgttgtc
    12001 cataaggtta caattgtacc acgcggccgt gcaggcggat acatgattgc acttcctaaa
    12061 gaggatcaaa tgcttctatc taaagaagat atgaaagagc aattggctgg cttaatgggt
    12121 ggacgtgtag ctgaagaaat tatctttaat gtccaaacta caggagcttc aaacgacttt
    12181 gaacaagcga cacaaatggc acgtgcaatg gttacagagt acggtatgag tgaaaaactt
    12241 ggcccagtac aatatgaagg aaaccatgct atgtttggtg cacagaatcc tcaaaaatca
    12301 atttcagaac aaacagctta tgaaattgat gaagaggttc gttcattatt aaatgaggca
    12361 cgaaataaag ctgctgaaat tattcagtca aatcgtgaaa ctcacaagtt aattgcagaa
    12421 gcattattga aatacgaaac attggatagt acacaaatta aagctcttta cgaaacagga
    12481 aagatgcctg aagcagtaga agaggaatct catgcactat cctatgatga agtaaagtca
    12541 aaaatgaatg acgaaa
//
LOCUS       DAVLVZ010000052        12392 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000052 DAVLVZ010000000
VERSION     DAVLVZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.052"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            106..270
                     /locus_tag="VYN77_001532"
     CDS             106..270
                     /locus_tag="VYN77_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1558976.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFAGRK"
     gene            354..920
                     /gene="amaP"
                     /locus_tag="VYN77_001533"
     CDS             354..920
                     /gene="amaP"
                     /locus_tag="VYN77_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1558977.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLQKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            932..1102
                     /locus_tag="VYN77_001534"
     CDS             932..1102
                     /locus_tag="VYN77_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1558978.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1141..1749
                     /locus_tag="VYN77_001535"
     CDS             1141..1749
                     /locus_tag="VYN77_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1558979.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1780..1983
                     /locus_tag="VYN77_001536"
     CDS             1780..1983
                     /locus_tag="VYN77_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1558980.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2093..2371)
                     /locus_tag="VYN77_001537"
                     /pseudo
     CDS             complement(2093..2371)
                     /locus_tag="VYN77_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2569..2856
                     /locus_tag="VYN77_001538"
     CDS             2569..2856
                     /locus_tag="VYN77_001538"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1558981.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANIGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3134..3904)
                     /locus_tag="VYN77_001539"
     CDS             complement(3134..3904)
                     /locus_tag="VYN77_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1558982.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSILGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3919..4767)
                     /locus_tag="VYN77_001540"
     CDS             complement(3919..4767)
                     /locus_tag="VYN77_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV1558983.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGLQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4764..6239)
                     /locus_tag="VYN77_001541"
     CDS             complement(4764..6239)
                     /locus_tag="VYN77_001541"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV1558984.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKEMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYVSDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTPTKTGDSQS"
     gene            complement(6562..7917)
                     /locus_tag="VYN77_001542"
     CDS             complement(6562..7917)
                     /locus_tag="VYN77_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1558985.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGTFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7927..8244)
                     /locus_tag="VYN77_001543"
     CDS             complement(7927..8244)
                     /locus_tag="VYN77_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1558986.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8261..8578)
                     /locus_tag="VYN77_001544"
     CDS             complement(8261..8578)
                     /locus_tag="VYN77_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1558987.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8593..9489)
                     /locus_tag="VYN77_001545"
     CDS             complement(8593..9489)
                     /locus_tag="VYN77_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1558988.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9492..10754)
                     /locus_tag="VYN77_001546"
     CDS             complement(9492..10754)
                     /locus_tag="VYN77_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1558989.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            11034..12212
                     /locus_tag="VYN77_001547"
     CDS             11034..12212
                     /locus_tag="VYN77_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1558990.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLISQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         4302 a         2463 c         1897 g         3730 t
ORIGIN      
        1 tttttgatat taaaataaaa aaggagggta ggcagtgttg tgatcaatta ttgctggagg
       61 tcttattggt ctcttggcag gtaaaatcac taaaaaagta gttctatggg aatcatcgca
      121 aatgtattcg ctggtttagt cggggcatat gcaggacaat ctcttttagg tagttggggt
      181 ccatcaatcg ctggaatggc tttgctccca tctattgtag gtgcagcgat tgtgattact
      241 gtagtgtcat tctttgcagg tagaaagtaa acttttcgcc agtaaagtta gcaaactatt
      301 tttaaatcaa tgacgggaaa aatagtttaa atgttaaatc gaaaggattg tatatgtcaa
      361 aagcaaagaa aatatgtttc attattttct gtattttaat cttgacaatt ttccttcctg
      421 ttttgataga ttatcatcaa gttagtgatc taggtattca tctacttagc tggagacaga
      481 actccgtagt tgaattctat cttgctagat atgtcttttg ggggacagtg gttctatcaa
      541 ctttagtttt attatccatt ttagttgtga tgttttatcc taaacgttac ttggaaatcc
      601 aacttgaaac taaaaacgat acattaaaat taaagaattc ggcaatcgaa ggttttgtta
      661 gaagtttggt gagtgatcat agattgatca agaacccaac tgttcatgta aatttacaaa
      721 aaaataaatg tttcgttcat gtagaaggta aaattcttcc ttcagacaac atcgctgaca
      781 gatgccaaat aattcaaaat gaaataacta atggattgaa gcagtttttt ggtattgagc
      841 gtcaagtaaa acttgaagtt gcagtaaaaa attaccaacc aaaacctcaa aacaaaaaga
      901 ctgttagtcg tgtgaagtaa ggaagtaaaa aatggaatgg cttaaacaat atcgatatcc
      961 aattatcgct ggtctcatag gcgtatttct ggcttgtttg attgtctcct ttggcttctt
     1021 caaaacaata tttgtattga ttttaggagc actgggagtt gcagctggat tatatatcga
     1081 aaaaaactat atagataaat aaaaaaataa aaattactaa tttaattaaa ggagtttcat
     1141 atgtcaaacg aaaaaaacac aaacactaac gtagaaaaga aagatgctac tgttgtagct
     1201 cacgaaatca aaggggaact tacttacgaa gataaagtta tccaaaaaat cattggtctt
     1261 tcactagaaa acgtttcagg tcttttggga atcgatggtg gtttcttctc aaatcttaaa
     1321 gaaaaaatcg ttaacagcga tgacgtaaca agtggtgtta acgtagaagt tggtaaaaca
     1381 caagttgcag ttgacttaaa cgttattgtt gagtaccaaa aaaatgttcc agctttatat
     1441 tcagaaatca gagaaatcgt atcttcagaa gttgctaaaa tgactgactt ggaaattgtt
     1501 gaaatcaacg taaacgttgt cgacatcaaa actaaagaac agcatgaagc agactcagta
     1561 agccttcaag atcgcgtatc tgacgttgct gaatcaacag gagaattcac ttcagaacaa
     1621 ttcgaaaaag ctaaatctgg tcttggatct ggtttctcaa ctgttcaaga aaaagttagc
     1681 gaaggtgtag aagctgttaa aggtgcagca aatggtgtag tatctcacga aaacactcgt
     1741 gtaaactaag ataaaataaa tataacagga gaaattatca tgtcagtaga agaaaaatta
     1801 aatcaagcta aaggttctat taaagaaggt gttgggaaag ccatcggtga tgaaaaaatg
     1861 gaaaaagaag gagcagctga aaaagttgtt tctaaagtaa aagaagttgc cgaagacgct
     1921 aaagacgctg tagaaggtgc tgtagaaggt gttaaaaaca tgttgagtgg cgacgataaa
     1981 taaggttaaa agttacttta tctttttggt aacattagtc aaaagagtct gagtcaagat
     2041 gattctcaga aaacaaaaag ctagagatta ccaattgcgg aactctagct ttttaatttt
     2101 gcctctttct cttattatat ttcagcaggt tgttggccat gagtatgaat cccatgtcaa
     2161 ttctcacttg acgcttacct ctcagatgac atctcttata acccaaacaa acctttatct
     2221 gcccaaagac agatttcata tcaatcttac gtttagcgaa aatttgtcta cccttggaag
     2281 ataaaagtgc ctgatattct ttagttttta aacactggta acgttcattc atatacagtc
     2341 ccttttgagg ggctgattca ggttcataat cacagtcaac attgatttca aggctgtttg
     2401 ctttctatct cccctttttc atgatatatt ttcgtacagg tgcttcaata gtttctatag
     2461 taaaatgaac caaaaatagt acacgttgcg gtataatgtt cttgtggcat attcattaga
     2521 ttttcgtaaa aagttctctc ctatttgtga gcgaacaggt atagtcaatt gattgtcagt
     2581 ttgaagacca agagtcgtaa ggcaaaggat atggcagaat ccatccaagg gtggctggca
     2641 caatttctag tgaacctctt taaatctatt acgtttgact gtggaaaaga gttttcaaaa
     2701 tggaaagata tttccaatca tcacgatagt gagagctttt ttgcgaatat aggatgtcca
     2761 agacagcggt gtctcaacga acattcaaat agacttttaa ggtgtcatga cttgccaaag
     2821 cagacagatt tcaacgaagt cagtcaagaa ttttaggctg gacaaaaact caatttcagg
     2881 agaaaatgaa ataaatattc ccacaataaa acgcatagta tcaaagtttt tccaacacct
     2941 gatattatgc attttttaat tttaaagact ttctcccagc ctcaagaaat cacatcccac
     3001 ctaaatcctt tggataccaa atacctcatc aggtattttc tgagttggta agactgtcta
     3061 gcttaatttg acaaatggag agcgttaaat ccagttttta caggaagtct atttaaatat
     3121 tttcacactg accttacaaa atatgaaata aattatataa agtcattccc atgacaaaga
     3181 ataacatccc tttaaaacag tatcttaccc aattttcagg taagactttt gatacaaccg
     3241 tccccaatac ccctccgcat atagaagcaa gaaaaataac tggaacaggt gctaaatgat
     3301 actgatttaa acctgtaacc acaacaattg tagccaaatg agacagctgg gaaaagaaaa
     3361 taattgcaag agaatacatc gttgcttctt ttatcgaaat agaaaaaaac accatcaata
     3421 aagatacatt gagcggacca ccaccaatcc ctaaaaaact agaaattaag cccaatagaa
     3481 gaccacaaat catatagaat cctatcttat caaattgata agttttttta aaatctgtta
     3541 aaacaaatga tacaagtagc atcacaaata gtaaaatcat ttgaacaatg gatactagcc
     3601 gtactgataa ctgagataac aaaacttgaa aaataagatg acctagaaat cctcctaaaa
     3661 tagaagaaaa ggataccgta aaaatcaatc tccattttat ctctttactt tgtgaaaatc
     3721 ttttgattgt tgaaataatc gccattataa aaacagagaa actagaataa aaagcaatat
     3781 cactgaccga atggtatcca aaactatcca taagtggctt aattataatt ccaccaccca
     3841 tgcctgatat agaacccaat ccactagcta ttataatgat tagaaaataa ataatctcaa
     3901 ccacttttta agtccatcct attctttcaa acatctaaaa cctagatggc tactagtgga
     3961 agtagaaata catccatttc tagcagctac tcggtatcga ttacaataag aacaatgaca
     4021 taaaaaggaa cctcctctga tagcatactc ctcatcttgt atcccatatt ttggtaactc
     4081 atagtcatct tcattaaaac tagctaataa ggtatatctc ggattgcgac accattccca
     4141 aacattccca atcatttgat atagaccaaa atcattaggt aaaaattcat agacaggagc
     4201 cgttcctata aatccatcta aggcagtatt ttcataagga aatcttcctt gccaagtatt
     4261 ggcatgataa tatccacctt ctaaaagagt atctccccaa ggatattcag attgtcttcc
     4321 tcctctagca gcatattccc actgtgcttc agtcggtaaa gacattccgc tccaattaca
     4381 gaaagccaat gcatcttcta aagcaacatg aactactgga tggtcttcta aacccactag
     4441 attactgttc tcaccgtagg gatgtttcca gcaagcgttc gaaacttgta accaccacgg
     4501 agccccagca ggatgaggat atccctccct ttctgcctcc ggaacgaaca atataaaaac
     4561 aaaagaccac tcttgtcgct cagctagtgt tttatagcct gtttctttca caaattgagc
     4621 aaattcttga ttcgtaactg gagtatccgc aatggaaaaa tcctttacac taacagcgac
     4681 ttgaagacct tcaaaatcat ctaaaaagcc ctcttcagaa tttgttccca tcataaatgt
     4741 cccacccttg atttttttca ttttcatgat tgactatccc ccgttttcgt tggagttggc
     4801 gttattttag aaagttcaac aggaactaac tcttcatcca caacaaaacc ttcttctcta
     4861 tatctaagaa agtctactaa ttttttcttc atctgacgaa caataggttg atatttttca
     4921 cttggataca aatcattcat ttcatgcgga tcttttttca tatcaaataa ctgataatga
     4981 tttaaaacag gaaaccagat aaatttccac tggtctgtca aaatatattg actagaatct
     5041 tttcctaaag catgttcacc atgaaattct gttcgccacc cctcgtactg tccaaataat
     5101 aaattcttaa cactccgtcc atctagttca tctgtagtag ttcctcctgc caaatccacg
     5161 agtgaaggaa aaatatcttg aattttcact aattgcttga ttgttcccct attaccagct
     5221 atcaatccag ccggatcgta aataaacgaa ggaatgtgga tacttccctg ataaggatat
     5281 ccctttctaa acagataatg ttcaccaagc tgatctccat gatccgaaac aaaccagata
     5341 atcgtatctt tatcatgacg aaattctttt agggcagtca aaaagcgact aatttgatgg
     5401 tctatatgag taatcaatcc aaaataagca gcaaccattc ttctttgatc atcttccttt
     5461 aatttccctc ttaaagcata aatagacgga atttgctttt ctaatacttc ccaattcccg
     5521 atatgcaaat cgagaaattg aggcaagcgt tccatataca tatcaaaata gtattttggg
     5581 ggatttaatg gagcatgtgg tttttcaaaa gacattttga gaaaaaaagg aaccgttgga
     5641 tctcttcttt gtaaaaatga aatggactca ctcacaaccc agtttgttgg atgtaacttc
     5701 tcatctttat cccaaggtct agcttcccaa gaattacaat ccattccgtc atcaatcaaa
     5761 tcaacatcat aaccaacttt accctttaaa aaggctagat agtcactaac ataatcaaat
     5821 tgacttccat aagccttatc atactttcta tcaacatgta gatacccatc gtgaagtaag
     5881 acatgatcaa acccaagtct ctgtctggaa ggaaaaacat gcatcttacc aatacactct
     5941 gtttgataac ccatctcttt aaagactttt ggaagagtat tagtaaaatt ccaaggcacc
     6001 tcatcttgat aaccaacacg tccactttta tcttggtcta agccagtcaa taaagctgcc
     6061 ctagcaggaa cgcaagaagg aactggagaa taggcatttt caaaattata acctacacta
     6121 gccatcatat ctaatgttgg agtgctaact agcttatcct tactattcaa ggataaggcg
     6181 tctgctctca tttgatctac aacaatcaaa ataatatttg gctgttttgt ctgaaccata
     6241 aaatctcctt tctaatatgg caaaagaggc acaagaagat atctaccttt actgcacccc
     6301 tttctatatc aatctctcta tataaagcaa taacattctt gttatgtttt atagaacaat
     6361 gaactaaaat atgactaaat cgattaggaa attcaaatca ttttctagta ctgttttaga
     6421 agttacagtg tactattcca acttcaataa attataaacc tttgtctaat aacaatttta
     6481 gtggagataa gaaatcctac acctaactca tcttacacgt aatctatttc tattttatca
     6541 caaaaaacgc aagtaagacc attaactcaa ttcagtttta tctgccattt tcacaaatgg
     6601 gaaataagtc aagacactaa taatcaaaca aacaactgat aagatgatgg cacgccaatc
     6661 aaatgctgta gagaagaaac catataaaat tggaggcatt acccaagtaa cattttgtgt
     6721 aacaggtgaa acaagacccc agcttgttgc ccagtaagct accgttgcca tgaaaaccgg
     6781 gctaagtaca aatggtataa atagcaaagg attcaagaca actggtaaac cataattgac
     6841 cggctcacca atattaaaca gaactggtgc tagaccaagt ttagcaactt ttcgataatg
     6901 actgtttctt gaaaaaatta aaatagcaag tactaatcct aatcctccaa accagacaaa
     6961 cgtcccaaaa gacccacttg tccatatata aggaatcggt tcaccttttt ggaaagcatc
     7021 cagattcgct aacatagcaa ctccaaatag cccttccatg atgggagcca atacatttcc
     7081 tccatggaga ccaaaaaacc agaataactt attcaaaaag atcatcagaa taactgcaaa
     7141 gaaactttga gacaaaccta gtaatggcgt ttgtaacacc ttgtaaaccc aatcaatcaa
     7201 taagtcattg ctaagtaaat ggaaaacata agtcaagatg gctactatat acatcgccat
     7261 aaatcctgga atgatagaag tgaacggctt agcaatcgca gggggaactg aatctggtaa
     7321 cttgattacc cagttctttt tcattacttt acagaaaata atagaggcta aaaatccaat
     7381 catcatggct gtaaagtagc ctctggcatt aatatggttt cctggaatca cattcccaat
     7441 agttaccatc agatttttac catcaaatgc tagattatca attccttgta agatctgatc
     7501 taatttcaca tctcctacat ttgccaaagg gaaactcttt gtaactgtac ttccaatcga
     7561 aatgacaaac gaagcaagtg ataccaaacc agcagaaact gtatcaacct tgtaaatctt
     7621 agcgatattc actcccaagc aatagatgaa caacaaggaa acaattggta tacttccctt
     7681 gaataccaaa ttattgatgt caacaagcca ctgaaaggtt ttcgtaatac tttctaggtg
     7741 aaattgttgt ggtaaatcca ctagaaaagc atttaataac aaagcaatgg aacctgtcat
     7801 aataacaggc atagtcccca caaatgaatc acgtaaagca actaaaaact tctgattccc
     7861 aactttcatt gcaattggag aaagtttttc ttgcatttta tccaacattg tattcatact
     7921 aaactcctaa tttttttcaa atcgttcgta tacttctacc acttctttcg ccaagtcaac
     7981 aaaagtcaga ctcgtcatta aatgatcttg cgcgtgtacc atcaagaggc taattgatgt
     8041 ttcttccccc tgtgcctctc ttgtcaacat ttctgtttga gccttatgcg cctgtaataa
     8101 ggcagcattc gcatcagcta atcttcgatt ggctttagag aaatcccctt tttttgctgc
     8161 ctgaatagcc tccatagcat tacttttggc ttccccacca tacataatta gccccattgc
     8221 agattctaaa ttactttcat tcatagcatt ctcctcaatc ttagtctaat aaactttcag
     8281 ctaaatctaa gactttagaa ccattcatca ttccataatc tgtcatcgga ataaccgcca
     8341 caggaatctg tctatctttt agtttttctt gaaagtcccc tagtaaatag cgaacttgag
     8401 ggcctaaaag caacacattt acttcctttg ttgctacaat ttcttctgct tcaggagctg
     8461 gaactgcaaa aatttctgca tccaacccct tatcttctgc tgccttttgc atctttgtca
     8521 ctagcagact tgtactcata cctgctgcac atgctaacat aattgttact ttagccatag
     8581 cttactcctt tatcacctat ttactgacct agccaagcga gatactgtat gtctcactcc
     8641 tctaattgcc ttgctcaatg cataaatatt atcgactgtt gccagtcccc catatcctgc
     8701 gtcacctata tgttgaatat caactccaca aattttattt ctaagtgcaa tttccttgat
     8761 agtatctgta tcagatgttt cttggctagt accaatagca ctcagtacta gcccaccctt
     8821 actatgaacg agatcaacga cttcacgcaa ctcttggtca tgaaaggctg gaacggttcc
     8881 aactgctgga acaagtatca catccgcacc tgcttccaac aattgctctg ctacagaaag
     8941 ctctgccaca ggctcattca ctcctgcccc gtgcatctta cctgcaataa tcaaaccaga
     9001 aaagttttcc ttagcagttt gaacagcctt aatgatttct cgattgctaa ctcctgttcc
     9061 aggatttcca gtcaaacaga caaagtcaaa acctaactcc tctattcgat tcaatgtttc
     9121 aacactagca acacgacctg caacaatttc ctgtgtttcc tctagcatct ttgcagatgg
     9181 gtcaatcggt tccaaattta caccaattgg acaagcaaca agctggtgta agcgatgaat
     9241 aacatcatca cctgaactat ccaaagcata aattttaggc tcaaaaacat caacacaatt
     9301 caataaaatc atatcagctc caaaagctct agcaatttca gaattagtta tatccccaac
     9361 aaatgtctca cgagtaacta cattttcaga tagaacaact ctaccttcac ttgctaaaat
     9421 actttgtttt aattcttcag cattcatctg taatatctca gatggatttg cactaattaa
     9481 acgtttcatt ctcaatcctc caataactta aaccgttttt caatagctat tacaaatggt
     9541 aaataaagta agacatccaa aacaatcaat acaatttgta ataatgaacc agaaatactt
     9601 cctgtagcaa gtaaaccact tataacaggt ggcatagtcc aacctggatc tgtataagtt
     9661 agcggaacca aacctgatgc cattgctccc cacgctacta gtaagttaaa cattggagct
     9721 aagataaatg gaactagtaa caaaatgttc aaaacaatag gaacgccaaa cattgctggt
     9781 tcatttatat taaataatcc aggtataaca gttataggtg ccaaagtttt tagttgttta
     9841 ctagctttct tcttatgtgc taaatatcct aacgcaagaa ctaaacctat ggttgcacca
     9901 ccgccaccta tgaatacaaa attatccata aatacgctag taaaaatatt tggaagagtt
     9961 tctccagttt gataagctac tttatttgca tctagattag ctaaccataa cggtttcata
    10021 actgcattaa caacatttcc tccgtgtaat cctacaaacc aaaaactact attaaggaaa
    10081 attaagataa gaagaccaat aacattattt cctaaaagtc caagtggacc tcccaaagct
    10141 acttgagcaa tttcatgaag attaggtaaa cttaatttat ctaaagttgc ataaacaatc
    10201 aaccaaccaa caatagtcac tgcaccagga ataatggcac taaagctttt agatactgct
    10261 ggtggaacac catccggcat ttttatctgt atattatgat ttataaacca ttggtagata
    10321 tagccattaa tcaatcctaa aatcatagca acaaataaac cttttgatgc catgaaagcg
    10381 gtaggcattc ctgctccagc ctctcctcta atgaaaggcg taactgtaat aaaagatgat
    10441 aatgacacaa taccagcagg aataccatca actttgtatt gtctagccat aaagtaagcg
    10501 ataccaaaac tagctacaag accaagtaat ccaaaactac tatcaacacc cttccataaa
    10561 tagtcagcaa caccaatatc acccagatat ttttcccatc ctggaatagg aaaacttgca
    10621 ataaccatta gtaatgaacc tatcaacaat aaaggtatag ttagagtaat accgtctcga
    10681 atagaaacca acgctttatt attacccaat ctagccgcta ctggaagtaa cttttcttgt
    10741 aataattttt ccataacctt ttcctcttaa atatatttta ttatcaaaag ttatcaatta
    10801 tctttatttt ttgacgtcaa gttgttattt gattatcttt tgttatttat attttacaac
    10861 tataagaaag ccttgtcaag gatttgtgta aatatttgat tattttttat tatcaatata
    10921 taataaaaag agaccagtaa gcaatgaaaa gcttaaaaag attaacatct acatttatcc
    10981 atagcaaaaa atcataggat tttaagaatc tgacaactag gaagaaaact aatatgacct
    11041 tatctaagaa acaattacaa ctacgagcta aaatactgga aacagtttac acattaggtc
    11101 ccatttcacg aattgaaatc gctactaaaa caggaataac acctgcaaca actagcagta
    11161 tcacaaatga tctgattaaa gaaaacattc tcttagaact tggtgaagac gaacacgata
    11221 caagcgtagg gagaaaaaaa atcctgctag acattcaagc taaacgattt tactacattg
    11281 gttgtgaact atctgaaaaa catttcactt ttgctttagg agataatcta ggaaatatct
    11341 taaaggaaga gaaagaaatt gttactaaac agttaatcca agaaaaggga aatcaactca
    11401 ttagccagac actaaagcaa ttcttaaata actgctctga ttatgaaata gaagcaattg
    11461 gaatcgcgct acctggtcgc tatttagatg attataaaat aacaacaaac aatcctttat
    11521 ggcaacatat cgacttagaa atgattcaat cccactttga taagcctttg tttttctcta
    11581 acaatgttaa ctgtatggct ataggtaaac gcctatttag tcgccaacag aatgacccta
    11641 attttgctta ttttcatttt gctagaggta tgcactgttc ttatatctac gacggaaaca
    11701 tttatggaaa aggaaatcta atgattggag aaatagggca tacagttgtt tcatctgagg
    11761 gagaagaatg tagttgcggg cgcaagggat gcttacaaac ttttgctgga gaatcttggt
    11821 taatcaaaaa atcaaaaatt ctatatcatc aatctcccta ttctctactt cctagtttag
    11881 tcaaaaatgc cgatgatatt gatatccaag ttattttaac cgcttatcaa ttaggtgaca
    11941 ctggaataat cacacttatc catcaagcct tactctatct tagtcaaacc attttaaata
    12001 tcagtatgat gattgactct caaaaaatct acctacatag tcctttacta acaaatcaac
    12061 acatcatcca aaaactatat agtgaaatga actacaaacc caaactgctc tataatcgcc
    12121 tacctgaagt cattattgaa ccttacaatg attttacagc cgcacactca gctattgcac
    12181 tttgtttgta tcatacaatt ctacattcct aattttctat tacatttaat aaataggcta
    12241 aaaagaggga gtatatcata aaaagcgaac aaacctgttt tctgaaaatc agaattctgt
    12301 tttgttcgct ttctttatat ttggaattat actcctatct cccctttttc ataatgtata
    12361 gtaaaatgaa ataataacag gacgaatcga tc
//
LOCUS       DAVLVZ010000053        11881 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000053 DAVLVZ010000000
VERSION     DAVLVZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.053"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(46..468)
                     /locus_tag="VYN77_001548"
     CDS             complement(46..468)
                     /locus_tag="VYN77_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1558991.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            615..791
                     /locus_tag="VYN77_001549"
     CDS             615..791
                     /locus_tag="VYN77_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1558992.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            1192..3405
                     /locus_tag="VYN77_001550"
     CDS             1192..3405
                     /locus_tag="VYN77_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1558993.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFATEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(3627..4103)
                     /locus_tag="VYN77_001551"
     CDS             complement(3627..4103)
                     /locus_tag="VYN77_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1558994.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINTFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            4326..7529
                     /locus_tag="VYN77_001552"
     CDS             4326..7529
                     /locus_tag="VYN77_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV1558995.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNREKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLELSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            7910..8295
                     /gene="tnpA"
                     /locus_tag="VYN77_001553"
                     /pseudo
     CDS             7910..8295
                     /gene="tnpA"
                     /locus_tag="VYN77_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(8674..9303)
                     /locus_tag="VYN77_001554"
     CDS             complement(8674..9303)
                     /locus_tag="VYN77_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1558996.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(9313..10314)
                     /locus_tag="VYN77_001555"
     CDS             complement(9313..10314)
                     /locus_tag="VYN77_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1558997.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(10345..10986)
                     /locus_tag="VYN77_001556"
     CDS             complement(10345..10986)
                     /locus_tag="VYN77_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1558998.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(11005..11820)
                     /locus_tag="VYN77_001557"
     CDS             complement(11005..11820)
                     /locus_tag="VYN77_001557"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1558999.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         3726 a         2054 c         2564 g         3537 t
ORIGIN      
        1 atgttttttg aaaaagttca cctcaaagtt taccttattt tcaccttatt gttttagaga
       61 ctgtaaggca aattcaccga cggccatagg gactacatta gaagtattga cataaatctg
      121 tgttgtacct gtgtcgctat gtattagagc ttcagaaatg gcttctaagc tcattcctgc
      181 ttgtttagcg agcgttgctc cggtatgacg tagtttgtgt ggcgtagcgt gtgtaagctc
      241 tgggtgtcgt tttctgatag ttttcatctt attattgaga tagtcagaat gtaaaggttt
      301 attgatattg cctttggtat ctatgtaagt aaagatgaat tgttctggat tactgatgat
      361 accaaacttt gccagttcat atttttgttg ttttttccat gaagtcagaa gttgaagtaa
      421 gtcattagaa atagagaaaa tgggaaatgt ttccacaata aaacgcatat tatcagatgg
      481 ttaatgcgtt ttatatttgg gtttgaggct tataaaatag gataagcgta ctgaaaactt
      541 agatatgaca aacaaaataa aatttttaaa agctacagag aaacatggta taatgtatct
      601 aaaggaggta gcatatgtcc ttagatatag ataaagaaaa gatgacaatc atgggaatag
      661 cttttgaaaa ccgatccgtt tttaagagtg tttggtatgc tttaagtaca aatatgattg
      721 aaggatggcg acctacggtt agtgatgttg aaaaattacg agatgaagct cttgctctgg
      781 ggatgactta atgaagcgca actgttatga gtcttatgac tatgttgatc cggataattt
      841 atataccgat ccgggttctt cagttctaag aaataagcag gaaggaaaaa agcacgcgag
      901 cttgaatatc gtatgattgc tagtaagagt ttgaaattat ttatcaatcc tatcttagtt
      961 aatagtttaa aagtagtttg tttcacaact gcataagctt agaaaagttg aaaagtgata
     1021 actctttaat aaactcttgg tttgttaaaa gatattttgt ccactacatt ttttcaattg
     1081 actattattt gtcatagata taacagatag aacattgcat tcagaataat gaaaaacgca
     1141 tcaaaaatgg tgcgttctgt ttttatgcta ccataaaagg aggaaaacaa attgaaaatt
     1201 tttaagggag agttttatcg aatctctgta ttaacagaca agctagtaag gttagaatac
     1261 tctcaaactg gaagttttga ggatagaacg acacaactta tctataatag agattttggc
     1321 caagtttcgt tagattatat cgagacatca aacgtactag atattatgac ggactatttt
     1381 catctgcact ttaataaagg agaatttaac gccgaaaatt tatttataga attaaaagga
     1441 aattttgccg tatatggtag tcgctggtat tttggtgaat ctattgaaac gttaaaagga
     1501 acagctcgga ctctggataa ggcagatgga gcaatctcgt tagaagatgg aattattagc
     1561 cgaaatggta tagccttatt ggatgattct caaggattta tttgggatga acaatctggt
     1621 tatattgaga gagaaaatca aattgacctg tatttctttg cctatgggca tgattataga
     1681 ggagcaatca gagactttta ccatttgact ggttcaacac ccttgttgcc aagatatgct
     1741 ttaggcaatt ggtggagtag atattggcct tatacgtcgg atgaatactt ggatttaata
     1801 gacagatttg caacagagaa aattccatta tctatcggtg tgttagatat ggattggcat
     1861 ataactgaca ttccagctcg ttttggaagt ggctggacag gatatagttg gaatagaaac
     1921 ttaataccaa atccagaaca gttattgcaa caacttcatg atagaaagtt aaaactctcc
     1981 ttaaatgtcc atcctgctga tgggatacgg gcttatgaag aagcttatcc tcaaatcgca
     2041 aaacggttgg ggttaaatgt agaactagaa gaacctgcta tttttgattt ttttaatccc
     2101 tcttttaggg aagcctactt taaagatgtt cattatgagc tagaaaagca gggagtagat
     2161 ttttggtgga ttgactggca acaggggaca caaggcatgc tagatccact ttggctttta
     2221 aaccattatc actatcagga tagttgtaaa aatgcagaag gtggtttgat tttatcaaga
     2281 tatgcaggtc ctggtagtca ccgctaccct gttggttttt caggggatac tattatcagt
     2341 tggaattcct taagatttca accctatttt acagcgacag catctaatat cggttatagt
     2401 tggtggagtc atgatatcgg tggacatatg ttgggggatt atgacgaaga gctacaaact
     2461 agatggctac agtttggtgt ttttagtccg attactcgat tacatagttc tagaagtcct
     2521 tttaatagta aagaaccttg gtttttttca gaaacaacat ctaagattat gaagaaatac
     2581 cttcgtttga gacatcagat gattccttat ctatatacca tgaatgtaaa gacacatgag
     2641 gaaggtgccc cattaatcag tccaatctat tatttctatc cagagaatga tgagagctat
     2701 aatgttccaa accaatactt ttttggaaca gaactgatgg tggctcccat tgtagaaaag
     2761 atggatttga cattccaatc tgcaaaagta gatgtatggt tccctgaagg tgaatggtat
     2821 gacttctttt cagagaaaaa atacacaggt ggtgtgaagt taagtgttta tagggacatc
     2881 tcgactacgc ctgtgtttgc aaaaagtggt gcaatcattc ccttggttgg ttctgagata
     2941 ggtatgggtg ttgatttacc tgaggttgta gattggtatg tattcccagg gaaacaacat
     3001 tcttttgaaa tgcttgaaga tcaaaatggt caaagatata aaacaagatt atcaatcgat
     3061 tgggaaatgg gaatggtaga gttagcatta caaggagatt ctagtatcgt tccaagcaat
     3121 agaaaacata gaattcattt taaaggaacg aatgtgtcta taattgaatt gccaaataag
     3181 aatgatacag ctaaatttga atggaaagat aataaaagga catctttaaa tgacgaagtt
     3241 tttagactac taaagacagc ttctcttcca tatgaattaa aagatagatt gttaaatcaa
     3301 ttcatcaatg cgaaaaactc tcatgactta atgaatatct tgcatcatca ggataaggaa
     3361 ttgagagggc gtttgttgga aatgatattt actagccaaa actaattgaa ataattagaa
     3421 agtcatttgt ttagtgtata tgaagattct gtatggtatt cgttatcact ggtcacattg
     3481 aaaatgtgaa ataagcacct acaacataga ctttacaaca cttcatgttt catatcatgg
     3541 ggtgtttttc tatttaagtc aaagccaatc aaaaaatctc actattcatc aggttaaagg
     3601 aaacctaatc atagtgagat tgtgaattat agtagagttt gtatcgcctc ttcaatttgt
     3661 tttgggtctg tttttgaaga aaagcgttca aagacttgcc catcacgacc aatgagaaac
     3721 ttagcgaaat tccattcgat tcgttttcct agtgggccag atttatggtc ttttaaccag
     3781 acatagagag ggtctgcttc cttaccgttg accttaatct tggcaaaacg tgggaaggtg
     3841 gtttgaaaat gtaggctaca gaaggtgttg atttcctctg cgctgccggg tgcttgtccc
     3901 ataaactgat tgcaagggaa atccaatatt tcgaagccct gatcttgata gcgttcatag
     3961 agttcttgaa gtccctggta ctggggcgtt aaaccacatc cagtagcagt gttgacaatc
     4021 aagagaactt tgccacgata gctatccaag ggagttgctt ggttgttttg gttcaaaacg
     4081 gaaaaatcat aaagtgaagt catgagggcc tcttttcttt ttctacattg taacagtttt
     4141 tacttttttc gtcgagtcgg atggaagata ttgttgaaga ataagccctc aatatgatag
     4201 aaaccgttta caaataactt taaaaatggt atgatttaat taaaccggtt tactttgttg
     4261 ctataaatct ctaatttgag atttagttcc tattgatttt acaatatgtt tattggagtg
     4321 tatacatgca aacaaaaaca aagaagctca ttgtgagttt gtcttcactt gttttatcag
     4381 gatttttatt aaaccattat atgacagttg gagcggaaga aacgactacg aataccattc
     4441 agcaaagcca gaaggaagtt cagtatcagc aaagggatac aaaaaattta gttgaaaatg
     4501 gtgattttgg tcagacggag gacggaagca gtccgtggac aggaagcaaa gctcaggggt
     4561 ggtcagcttg ggtagaccag aagaatagtt ctgcagatgc ctcaactcga gtcattgagg
     4621 ctaaggatgg ggctatcact atctcaagcc ctgagaaatt aagggcagcg gttcaccgta
     4681 tggttcctat tgaagctaag aaaaagtata aactgcgttt caagattaaa acagataata
     4741 aagtcgggat tgccaaggtt cgtatcattg aggaaagtgg taaggacaag cgattgtgga
     4801 attctgcaac gacgtcagga acaaaggact ggcagaccat tgaagcagac tatagcccga
     4861 ctttagatgt tgataaaatc aagctggagt tattctatga aacaggaact gggactgttt
     4921 cctttaagga tattgagctg gtagaggtag cagaccagct ttctgaggat tctcaaacag
     4981 ataaacagct tgaggaaaag attgatttac caattggaaa aaaacatgtt ttttctcttg
     5041 cggactatac ttataaggta gaaaatcctg acgttgcttc agtcaaaaat ggaattttag
     5101 aacctcttaa ggaagggaca accaatgtca ttgtcagtaa agatggcaag gaagtgaaaa
     5161 agattccttt gaagattcta gcctctgtta aggatgcata cacagcccgt ttggatgact
     5221 ggaatggcat catcgctggg aatcaatact atgattctaa aaatgaacag atggccaaat
     5281 taaaccagga attggaagga aaggtagctg atagcctatc tagtatttca agtcaggcgg
     5341 accgcaccta tttgtgggaa aaattttcaa attataaaat gtctgcaaat ctgactgcca
     5401 cttatcggaa attggaggag atggccaagc aagtgaccaa tccttcttct cgttattatc
     5461 aagatgaaac tgtcgttcga acagtcaggg attccatgga atggatgcat aaacatgtct
     5521 acaatcgtga aaagagcatt gttgggaact ggtgggatta tgaaatcggt acacctcgtg
     5581 ccatcaacaa taccttgtct ctgatgaaag aatacttctc tgatgaggaa attaaaaaat
     5641 atacagatgt gattgaaaaa tttgtaccag atcccgaaca tttccgaaag acgactgata
     5701 acccatttaa ggctctaggt ggaaacttag ttgatatggg aagagtaaaa gtaatagctg
     5761 gtttactgcg taaggatgat caagaaattt cttctaccat tcgctcgatt gagcaagtgt
     5821 tcaagttggt agaccaaggt gaaggttttt atcaagatgg atcctatatc gaccatacca
     5881 atgttgccta tacgggtgct tatgggaatg ttttgattga tggcctgtct caactgttgc
     5941 cagtcattca aaagaccaag aatccaatcg ataaagataa aatgcaaacc atgtaccact
     6001 ggattgataa atcgtttgct cctttgctgg tgaatggaga gctgatggat atgagtcgtg
     6061 gacgctcgat cagtcgtgca aatagcgagg ggcacgtggc cgcagtagaa gtactaagag
     6121 ggattcaccg aatagcggat atgtctgaag gagaaaccaa acaacgtttg cagagtcttg
     6181 tgaagaccat tgttcaatcg gatagttatt atgatgtctt taagaatttg aagacttata
     6241 aggatatcag tttgatgcaa tccttgttaa gtgatgcagg agtcgcaagt gttccaagaa
     6301 caagttacct atctgccttt aacaagatgg ataaaacagc catgtacaat gcagagaaag
     6361 ggtttggatt tggcttgtca ctcttttcca gtcgtacctt gaattacgaa cacatgaaca
     6421 aggaaaataa acgtggttgg tatacgagtg atgggatgtt ctatctttac aatggcgatt
     6481 tgagtcacta tagcgatggc tactggccaa cagttaatcc atataagatg cctggtacaa
     6541 cagagacgga tgctaagaga gcggatagcg atacaggtaa agttttaccg tctgctttcg
     6601 ttggaacgag caaactagat gatgccaatg cgacagcaac catggatttc accaactgga
     6661 atcaaacatt gactgctcat aagagctggt ttatgctaaa ggataagatt gcctttttag
     6721 gaagcaatat ccaaaacact tcaacagata ctgctgcaac tacaatcgac cagagaaaac
     6781 tggaattaag taatccatat aaagtctatg tcaatgataa agaagcctcc cttacagaac
     6841 aagaaaagga ttatcctgaa acccaaagtg tctttttaga atcgtccgat tcgaaaaaga
     6901 atattggtta ctttttcttt aagaagagtt caatcagcat gagtaaggct ttgcaaaagg
     6961 gagcctggaa ggatatcaat gaaggacagt cagacaagga agttgaaaat gaatttctta
     7021 cgattagtca ggctcataag caaaatggag attcttatgg ctatatgctt atccctaacg
     7081 tggatcgtgc caccttcaat caaatgataa aagagttaga aagtagcctc atcgaaaata
     7141 acgaaaccct tcagtctgtt tatgatgcca aacaaggagt ttggggcatt gtgaaatatg
     7201 atgattctgt ctctactatt tccaaccaat tccaagtttt gaaacgtgga gtctatacta
     7261 ttcgaaaaga aggggatgaa tataagattg cctactataa tcctgaaact caggaatcag
     7321 ctccagatca ggaagtcttt aaaaagctag agcaagcagc tcagccacaa gtacagaatt
     7381 caaaagaaaa ggaaaaatct gaagaggaaa agaaccattc ggatcaaaag aatctccctc
     7441 agacaggaga aggtcagtca atcttggcaa gtctagggtt cttgctactt ggggcgtttt
     7501 atttattccg tagaggaaag aacaactaat ttgttcatca tctagatgaa ttacatgaaa
     7561 ctgtggaaaa tagaaaaaat ccgaagaagg ccaaatatct tcggattttt cttttaaaaa
     7621 aggataagtt gatacttatc cttttttact tcattatgtg actatagcgt tttgaaacta
     7681 gaataggacg tcataactgc taaaagattt ctataaattc atttgatttt cctaatcaat
     7741 ttgttcgtat cctatttcac tccactatat tagtccgtct cgctccgtga ggtgcgagac
     7801 aaaacaacca cccgctatgc gggtgcgcgt cgaaggttat accaaaaaaa ctccaaacgt
     7861 gatacaataa aggtgttcaa tccaattgta aagcgaaagg agaaaaaata tggcacaaaa
     7921 ggctcatagt ttatctcaca caaagtggag gtgttctatc acattgtgtt cacccctaag
     7981 tatagacgaa aagtcatcta taatcaatat agaagtagtt taggcgaaat atttcatcgc
     8041 ttgtgtagtt ataaaggtgt tgaaatgatc gagggtcact taatgccaga ccatgtacac
     8101 atgttagtca gtattccgtc gaggctaagt gtttcaagtt ttatggggta tttaaaaggc
     8161 aaaagtgcac tcatgatgtt tgacaaacac gccaatctca agtacaagtt tgggaatcgg
     8221 catttctggg cggaaggtta ttatgtaagt ccagtagggc ttaatgaagc cacaattaag
     8281 aaatatagtc aagattaaag aaattatcga gtggatgatt tcttcacgag tatgaaaatt
     8341 tgagaactag taaagtatga tatatagtaa aatgaaataa gaacaggaca aaatcgatca
     8401 ggacagtcaa atcgattttt aacaatgttt tagaagtaga ggtgtactat tccagtttca
     8461 atctactata gcactagata aattgagtgt aaaagaatat gaggatccct ttagggatag
     8521 tggtaagtaa taccaaagtc tcttaaagag gcaagtgacg agtcaagagc aataaggctt
     8581 gaacaacgtg aaagccagcg tctttaggcg ctggctggta atttgggctt atagctctgg
     8641 gataaaccac ccgtttgacg ggtggttatg attttatctg agtgtaacat actgttgggc
     8701 aatctcgctg atgcggtcaa agttgccttg ggaagcgagt ttattgagtt cgccaccaat
     8761 tccaacggcg tctgcaccag cagcgaacca ttgagggatg ttgtttagac cgactcctcc
     8821 ggttaccatt acggaaactt gtgggatcgg tgccttgact gcagagatat atgctggact
     8881 gagagcacta cctgggaaga gtttgatgat ttcactaccg gcttcaagtg cagtcgtgat
     8941 ctctgtgagg gtaatacagc ctggaatgta cggtgtgctg tagagattgc acattttcgc
     9001 agtttcagca tggaaagatg gagaaacaac gtaatttgct ccggctagaa tggcatctct
     9061 agcagttacg gcatcaagca cagtacctgc accgatacaa acactctgat cgtcctgata
     9121 caagtctaca agttccttga tgatttgtcc tgcatactga ttggtatagg cgatttcaat
     9181 agctttgata ccgcccttga tacaagcaat cgaggcttgc agtccttctt cctttgtatt
     9241 tcctcgaatg acagcgacaa ttttctgttt ttttagttca ataatcgtat ctgatttggt
     9301 catgtaattc tcctaacgaa tgatatcttg tgcatttgcc agtaaatttt caatactagt
     9361 tgcggaagtg gagagatggt ctcctggaag agtgcatttg agagttgcgc tcgcaactgc
     9421 aaagtcaatg gtagttttta gggaggaatg atggagtagt tggtaaaggg caccagatat
     9481 aaaggcatcc ccgctaccaa ttcgttgata gactgcagtt tttagttgga cagactcttc
     9541 aaatcgttct tccagagcat aggcttgata gacatttttg tcttgctcat cactagagcg
     9601 gagggtatgg aaaatggcct tgaaaccata ggcttctttt aaaagtcgca tgcgattttc
     9661 cacctcttct aggctagcac tgtctcttgg aaacatctct agattttggt catcaatcat
     9721 gagaggatca ataccgaagc aatagtcagt aaaacgtgca aacttagaaa attcatactt
     9781 ggcttctagg actgaaatca tctttgttct cagattgaga tccattgaaa cgacaattcc
     9841 tcggcgcttg gcttcttcca agagtaggag aaggatcgca cggacctctt gaccgatagc
     9901 tacggtgatt ccactaaaat gaaaatggct aatcccctga aagagagaat ccatatctag
     9961 catgtttggc cgaatctggc tgatactcgt atgcttacga tcgtagaaaa cttcactttg
    10021 acgacaacca aagccgttct ccaaatagta gaggccgatt cgatcgccaa gccgacagat
    10081 tgaactggta tcgatttggt gctgtttcaa gaatgtgaga aaacgatctc caatctcgtt
    10141 ggcagggagt gcggtaaaga ctttcgttga gatacccagg gcttgcaaat tacaagcgat
    10201 gttaatttct gatccgccaa aataagtcga actggcaaca tgatcgccga tactggtggc
    10261 atctaatggt gagattcgaa ttaatggttc gccaaacagt aatattttcg tcatccatta
    10321 gttcctttct ggtttagaat agctttactt ggctaatact tctttcaaat aagcagcgac
    10381 ttcatcatct tggcagtttt caaagaagta ttcttggaaa tatttgccag agatggtgtc
    10441 tttgaggaag tcttggtcga ttgttttaag gatggtcatc aaatcattgt gggtgatttg
    10501 tttcacctcg tttaagatac gagcgttgcg ttgttcaggg attacacgtt ctcttgggta
    10561 gccaccgccc atttcttcag caaataagcg ttcaaacatc aatttgaggg tcagttctgc
    10621 cccccagcca aatcctttgg cataagggat agacaaggcg ttaccaccat tgatttgaga
    10681 ataaaggtaa gcgtcagttg ggtccactgc tagaccacag acaacaccag ggaagctgtt
    10741 taaagcaagc atagccccta cacccgtacc acagccggta acaacaaagt caactgcctt
    10801 tgtatttaaa aggatggcag ccattagtcc gttctgcaca taagttaatt gactttctcc
    10861 ttcttctcca cgcataccat agttaaataa ttggtagcct tttttatctg tcgcttcttt
    10921 tagactatcg taaataatgt gattcttgct agcttgacta ttttcattga ttaatgcgat
    10981 tttcataaga tcttctttct atttttactc aggttgtttt ccgatgtagg ctaagatacc
    11041 gccatctaca tataggatgt ggccattgac aaaattgctg gcatcactag cgagaaagac
    11101 agcagggccc atcaaatctt caggatttcc ccaacgtgca gcaggtgttt ttgcaatgat
    11161 gaactggtca aatgggtggc gagaaccatc ttcttgcaac tcacgaagag gtgctgtttg
    11221 aggagtggca atataacccg gtccaattcc gttacattgg atattggctc caccgtattc
    11281 agacgcaatg ttgcgggtca acattttcaa gcccccttta gcagcagcat aagcgctaac
    11341 tgtttcacgt cccagttcgc tcatcatcga acaaatattg ataatctttc catgcccttt
    11401 ctttatcata gaaggaataa ctgcctttga aacgataaat ggtgcgttta agtcaatatc
    11461 gatgacctta cggaaatcag cggcgctcat ttcgcacatt ggaactcggc ggataatacc
    11521 agcgttatta acgaggatgt caatgacacc aacctcttgt tcgatttgct tgaccatggc
    11581 ttggataccg tcctcgtctg tcacgtcaca gacatatcca tgggcttcga tgccaacttc
    11641 acgataagct tcaattcctt tattgaccag atcttgattg atatcgttaa agacaatagt
    11701 agcgccggct tcagcgtagg atttggcaat agcaaatcca attccataag aagcgccggt
    11761 gatgagagca atttttcctt ttaaagaaaa ctgctcaatt gagaatgatg tatttgtcat
    11821 ttgatacttc ctttcaaatt tgttaagact tctctataac caaattatat tctagtaaac
    11881 c
//
LOCUS       DAVLVZ010000054        11805 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000054 DAVLVZ010000000
VERSION     DAVLVZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11805
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.054"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            114..767
                     /locus_tag="VYN77_001558"
     CDS             114..767
                     /locus_tag="VYN77_001558"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1559000.1"
                     /translation="MEHSSWHALIKAQLPEGYFRKINQFMEQVYSKGTIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            777..1241
                     /locus_tag="VYN77_001559"
     CDS             777..1241
                     /locus_tag="VYN77_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1559001.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTSDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1254..2522
                     /locus_tag="VYN77_001560"
     CDS             1254..2522
                     /locus_tag="VYN77_001560"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1559002.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGSETI
                     DATGHVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKVPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2536..2796
                     /locus_tag="VYN77_001561"
     CDS             2536..2796
                     /locus_tag="VYN77_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559003.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRICACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2807..4168
                     /gene="pdrM"
                     /locus_tag="VYN77_001562"
     CDS             2807..4168
                     /gene="pdrM"
                     /locus_tag="VYN77_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1559004.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASIYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     FKC"
     gene            4322..5290
                     /locus_tag="VYN77_001563"
     CDS             4322..5290
                     /locus_tag="VYN77_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1559005.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5306..6298
                     /locus_tag="VYN77_001564"
     CDS             5306..6298
                     /locus_tag="VYN77_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1559006.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNEVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6516..7559
                     /locus_tag="VYN77_001565"
     CDS             6516..7559
                     /locus_tag="VYN77_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1559007.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7623..9308
                     /gene="lpdA"
                     /locus_tag="VYN77_001566"
     CDS             7623..9308
                     /gene="lpdA"
                     /locus_tag="VYN77_001566"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1559008.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASSVPVASTSNDDDKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDCIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9372..10361
                     /locus_tag="VYN77_001567"
     CDS             9372..10361
                     /locus_tag="VYN77_001567"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1559009.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEITEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10426..11496)
                     /gene="xerS"
                     /locus_tag="VYN77_001568"
     CDS             complement(10426..11496)
                     /gene="xerS"
                     /locus_tag="VYN77_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1559010.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3505 a         2203 c         2647 g         3450 t
ORIGIN      
        1 gtattcaaat cgtatatata gtcgatttat taaatcttta gataaactgt aaaaagtggt
       61 ttccatagcc actttttgct ataatagagg cagtaaaaat gaaggagtca gctatggaac
      121 actcgtcttg gcatgctttg attaaggcgc aattacctga gggttatttc aggaaaatca
      181 atcagtttat ggagcaggtc tattctaagg ggactattta tccacccaag gaaaaggttt
      241 ttcaggctct cttgacaaca ctgcttgaag aagttaaggt ggtaattcta gggcaagacc
      301 cctatcacgg accaggtcaa gcgcagggct tgagtttttc cgtacctgac tctatcccag
      361 ctccaccatc cttgcaaaat atcttgaaag aattgtcaga tgatatcgga gttaagaaat
      421 ctcatgattt gacagcttgg gctgagcaag gagtcttgct tcttaatgct tgtttgactg
      481 ttcctgctgg acaggctaat ggtcatgctg gtcaaatctg ggagcctttt actgatgctg
      541 tgattcaggt ggtcaatcat ctagatagac cagtcgtttt tgtactctgg ggagcttatg
      601 cacgtaagaa gaaggcctta gttaccaatc ctcatcactt gattatcgaa tcagcccatc
      661 caagtccttt gtcggtttat agaggatttt ggggttccaa gcccttttct aaggccaatg
      721 cattcttaaa agagacagga caagagccaa tcgattggct tagataagga gaaaatatgc
      781 ctcagttagc gacgatttgc tacattgata atgggaaaga actgctcatg ctccatcgta
      841 ataagaaacc caatgatgtc catgaaggga aatggattgg tgtgggtggt aagctagaga
      901 gaggagagac gccccaggaa tgcgcggcgc gtgaaatcct tgaagaaaca gggctcaaag
      961 ccaagccagt tctaaaaggt gtcatcactt ttcctgaatt tacatcagat ttagactggt
     1021 acacctatgt ttttaaggtg acggagtttg agggcgactt gattgactgc aatgagggga
     1081 cgctagaatg ggttccctat gatgaggttt tgagcaagcc gacttgggaa ggtgaccaca
     1141 cctttgttga gtggctttta gaggataaac ccttcttttc agccaagttt gtttatgatg
     1201 gggataaatt gttggatacc caagttgatt tctatgaata aaggagaaag cagatgctac
     1261 taatcaaaaa tggtcgtgta atggatccca agtctggttt ggatcaagtt tgtgatgtct
     1321 tagttcaaga tgggaaaatt atcaaaattg cgcctgagat cacggaagaa ggatcagaaa
     1381 cgattgatgc tactggtcat gtggttgctc ctggtttggt cgatattcat gtgcatttcc
     1441 gtgaacctgg tcaaacacat aaagaagaca ttcatactgg tgccctagca gccgctgcag
     1501 gtggttttac tactgtcgtc atgatggcta ataccagtcc aaccatttca gacgtggaga
     1561 ctttgcaagc agttctccag tcagctgcca aagagaagat taatgtcaag acagttgcga
     1621 ccattactaa aaactttaat ggtaaaaact tgactgactt taaggcactt ttagaagctg
     1681 gtgcggttgg tttctctgat gacggtattc cgcttgagag cagtaagatt gtcaaggaag
     1741 ccatggagga agccaaaaaa ctcaatacct ttatcagcct tcatgaggaa gatccaggtt
     1801 tgaacggtgt tcttggcttt aatgaaaata ttgctagaga acatttccat atctgcggtg
     1861 ctactggggt ggctgagtac gctatgatgg cgcgtgatgt catgattgcc tatgcaacta
     1921 aagcccatgt tcacatccag catttgtcta aggaagaaag tgttaaagta gtggagtttg
     1981 ctcaggggtt aggtgcagaa gtcacagcag aagtagcgcc acagcatttc tctaagacag
     2041 aagcacttct tttaacacaa ggtagcaatg ctaagatgaa tccaccgctt cgtttggaat
     2101 cagaccgtcg tgccgttatc gaaggtctca aatcaggtgt catcacagtt attgcgactg
     2161 accacgcgcc tcatcatgta gatgaaaaaa atgttgagga tattaccaaa gtaccatctg
     2221 gtatgactgg cttagaaaca tccctgtctc tcggcttgac ctatttagta gaagctggtg
     2281 agttgagctt gatggaatta cttgaaaaaa tgacatacaa cccagccaag ctttacaact
     2341 ttgaagcagg ttacttggct gagaatggtc cagcagatat cactattttt gatgccaagg
     2401 ctgaccgcct tgtggactcc cattttgctt ccaaagcagc taattcacca ttcatcggtg
     2461 aaaccttaaa agggcaggtt aaatatacca tctgtaaggg acaaatcgtc tatcaagctt
     2521 gataggagtt agaatatgaa ttattttcga aaacgtaggg agagacaagc gaaatctaat
     2581 agtggaattt actgtcccgc agctaatcgt tccaaaattc aatatgaaac taaagaaaaa
     2641 gctgaccatg cggttcaata tgataacgga ggtttagtta gaagctatta ttgtcgtata
     2701 tgtgcgtgtt ggcatactac ttcaaaatct aataaacctc cgctaagttt gaaaaactat
     2761 ggtctgaaac tctttggttt agataaatca gaatagaaag agaagtatgt ataagacaaa
     2821 gtgtttacga gagaagttag tattattttt aaaaattttc ttcccaatcc tgatctacca
     2881 atttgccaat tattctgcct cttttgttga tactgcaatg acaggtcaat acaacactat
     2941 ggacttggct ggtgtatcta tggcaaccag tatctggaat cctttcttta catttctaac
     3001 agggattgtg tcagccttgg tgcctatcat tggtcaccat cttggtcgag gcaaaaagga
     3061 agaagttgcg tctgattttt accaatttat ttatttggcc ttgggcctat ctgtggtctt
     3121 gctggggatg gtacttttct tggcaccaac aatcttgaat catattgggt tagaagcagc
     3181 agtagcggca gtagcggttc gctatctttg gtttttatct atcgggatta tccccttgtt
     3241 gctctttagc gtcattcgtt ccttactgga ttcgctgggc ttgaccaaac tgtccatgta
     3301 cctcatgctt ttgttactcc ctctcaatag cggatttaac tatctcttga tttacggtgc
     3361 ctttggtgtt ccagaactgg gaggggctgg tgctggttta ggaacatcct tggcctactg
     3421 ggtcttgctt gggatttctg ttctggtttt atttaaacag gagaagctca aagccttaca
     3481 ccttgagaaa cgaattccac ttaatatgga taaaattaag gaaggagttc gtttaggtct
     3541 gcctattggg ggaactgtct tcgcggaagt ggctgtcttt tcagtggttg gcttgattat
     3601 ggctaagttt tcgtccttga ttatagctag tcaccagtca gctatgaact tttcaagtct
     3661 tatgtacgcc tttcctatga gtatctcatc ggctatggct attgtcgttt cctatgaagt
     3721 gggagccaag cgatttgatg atgcgaaaac ctatattggt ctaggaagat ggactgccct
     3781 catttttgcg gccttcacct taaccttcct ttacattttt aggggaaatg tggccagtat
     3841 ttatggtaac gacccaaaat ttatcgattt gacagcgcgt tttttaactt atagtctttt
     3901 cttccagtta gcagatacct ttgcggcgcc gcttcaggga attttgcggg ggtataagga
     3961 tacagttatt cctttttacc ttggtttgct tggttattgg ggcgtagcaa tccctgtggc
     4021 tacgctattt gattccctaa cagattttgg agcttattct tactggatcg gcttgattat
     4081 tagtttgatt gtgagtgggg cgctctaccg ttggcgttta actgtgatta tgaagagatt
     4141 tgaatcttta gcaaaattca aatgctaaaa agtttgtgaa aaaatattct tgacaagaaa
     4201 atcccttgtt ttcacggggt tttctttttt attttgtgaa atttctttta gaaaaatttt
     4261 gacagtattt gccaaaagcg gtaaaatagt aaggtaagaa gaaagttaga aaggcaagaa
     4321 gatgtcaact ttagataaaa atcttttgct agaaatgttc cgtaagatgg aagaaatccg
     4381 tcgcatggac ttaaaaattg cacaattagt aaagaaaggg aaagtgccag gaatgacgca
     4441 cttttctgtt ggtgaagagg cagctaacgt gggggctatg ttagctctca atccagatga
     4501 tctgattacc tcaaaccacc gtggacacgg gcaagctatt gctaaaggga ttgacctcaa
     4561 cggaatgatg gctgaaatcc ttggtaaata cactggaacc tgtaaaggga aaggtggatc
     4621 tatgcatatc gctgaccttg atgctgggaa ccttggtgcc aatggtatcg taggtggtgg
     4681 tatggggatc gctgtcggtg cagccctcag tcagcaaatg caaaataccg gtaaaatcgt
     4741 tgtctgcttc tttggagatg gtgcgaccaa tgaaggtgtt ttccacgaag cagtgaacat
     4801 ggcttctatc tggaacctgc cagtcatttt ctattgcatt aacaacggtt acggtatctc
     4861 tgcggatatc aagaaaatga ccaatataga acatatccat caacgtagcg ccgcttatgg
     4921 aattcctgga atgttcatcg aagacggtaa caatgtcatc gatgtctatg aaggatttca
     4981 gaaagctgtg gaccatgttc gcagtggcaa tggtccagtc ttgattgaaa gtgtaactta
     5041 tcgctggctc ggtcactcat catctgaccc tggtaaatat cgtacgcgtg aagaagtgga
     5101 attgtggaaa caaaaagatc caatcgaaaa cctccgcaat taccttattg aaaataacat
     5161 tgcaagtgcc gaagaattgg aagaaatcca agcgcaagta aaggaagcag tagaagcttc
     5221 tgttaaattt gcagaggaaa gcccattccc accgcttgaa tcagcctttg aagatattta
     5281 cgcagactaa ggagaaagag ataaaatgga aacaaaaaca atgtccttcc gtgacaccat
     5341 tatccttgct atgtctgagg aaatgcgtcg cgatgaaaat gtgttcttga tgggagaaga
     5401 cgtcggtgtc ttcggaggag acttcggtac ttctgttgga atgcttgaag aatttggtcc
     5461 agaacgtgtc cgtgactgtc cgatttctga agctgccatc tctggagcag cagcaggagc
     5521 agccatgaca ggacttcgtc caatcgtcga tatgaccttc atggacttct ctgttattgc
     5581 catggacaat atcgtcaacc aagctgctaa aacacgttac atgtttggtg gtaaaggtca
     5641 ggttccaatg actgttcgtt gtgcagctgg taacgaagtt ggctctgccg cccagcactc
     5701 acaatctcta gagtcttggt ttactcacat tccaggactt aaggttgtgg cacctggaac
     5761 acctgcggac atgaaaggcc tgcttaagtc ttctatccgt gataacaacc cagttattat
     5821 acttgagtac aagtcagaat ttaaccaaaa aggggaagtg ccagttgatc cagactacac
     5881 aattccactt ggggttggtg aaatcaaacg ccaaggaacg gatgtaacag ttgttactta
     5941 tggaaaaatg cttcgccgtg tggttcaagc tgctgaagaa ttagcagaag aaggaatttc
     6001 agttgaaatt gttgacccac gtacccttgt tccgcttgat aaggatatca tcataaactc
     6061 agtgaagaag actggtaagg ttgttctggt caacgatgcc cacaaaacaa gtggctatat
     6121 cggtgaaatt tcagctatta tttcagaatc agaagcattt gactatctag atgcaccaat
     6181 tcgccgttgt gcaggagaag atgtgccgat gccttatgca caaaacctag aaaatgcaat
     6241 gattccaaca gttgaaagta tcaaagatgc aatccgaaaa acttataaca aagaatagag
     6301 ttacataaga taagttatgt aaataaaata aagctgacga gaaactttgt atcttttagg
     6361 tgcagagctc ttcgtcatta atatcttaga ttagagcacg ggctaaaagc tcggaaaaaa
     6421 gataaatctc cttggcttca tcgaagccat cgtcgatttc ctatttttca gtcgcttttg
     6481 acgccctttg tatcataaat agaattggag aggtcatggc tgatgacaag ctaagagcga
     6541 ctcctgcagc tagaaagtta gcggatgatt tagggataaa cctctacgac gtttctggct
     6601 caggtgcaaa cggtcgtgtc cacaaagaag acgtggaaac ttataaagac acaaacgtgg
     6661 ttcgcatttc gccacttgca aaacgaattg ccctcgaaca taacattgct tggcaggaaa
     6721 tccaaggaac cggtcatcgt ggtaaaatca tgaagaagga tgttttggcc ctgcttcctg
     6781 aaaatatcga aaacgatagc atcaaatctc ctgctcagat tgaaaaagtg gaagaagtcc
     6841 ctgataacgt aacaccatat ggtgaaatcg agcgtattcc aatgacacca atgcgtaagg
     6901 ttattgccca acgcatggtt gaatcttact tgactgcgcc aaccttcacc ctcaactatg
     6961 aagttgatat gactgaaatg ttggctcttc gtaagaaggt tcttgagcca atcatggaag
     7021 caactgggaa gaagactact gtaacagacc ttctttcgct tgcagttgtt aagactctta
     7081 tgaaacaccc atacatcaac gcttcattga cagaagatgg caagactatt atcactcaca
     7141 actatgtcaa tcttgccatg gcagttggga tggataatgg attgatgaca cctgttgttt
     7201 acaatgctga gaaaatgagt ctttcagaac tggttgtagc ctttaaggat gttattggcc
     7261 gtaccttgga tggtaaattg gctccaagtg agttacaaaa ttcaacattc acaatcagta
     7321 atttgggaat gtttggtgtt cagtcctttg gtccgattat taaccaaccc aactcagcta
     7381 tccttggtgt cagttcgaca atcgagaagc cagttgtcgt caatggtgaa attgtgattc
     7441 gcccaatcat gagtttagga ttaaccattg accaccgtgt cgtagatggt atggctggtg
     7501 ctaagtttat gaaggacttg aaagagttga ttgaaacgcc aatctcaatg ttaatttaag
     7561 agcaagagta ttcattttta agatatagat aaaggaagga agacatggcc ttagaagtaa
     7621 ttatgccaaa agccggcgtg gatatgacag aaggacaaat cgtccaatgg aataaaaaag
     7681 tcggagaatt tgtaaaagaa ggagaaatcc ttttggaaat catgactgat aaagtcagca
     7741 tggaattgga agccgaagaa gatgggtact tgattgccat tctcaaagga gatggtgaaa
     7801 ctgtccctgt aacggaagtt atcggttacc ttggtgaaga aagggaaaac atcccaacag
     7861 ctggagcagc atcgccagaa gctagctctg tacctgtagc aagtacctca aacgatgatg
     7921 ataagagcga tgatgctttt gatatcgttg tgattggtgg aggtcctgct ggttatgttg
     7981 cagccattaa agctgcccaa ttcggcggta aggttgccct tgttgagaaa tctgaacttg
     8041 gtggaacctg cttaaaccgt ggatgtattc caaccaagac ctaccttcat aacgctgaaa
     8101 ttattgaaaa tatcggtcat gctgcaaatc gtggtatcgt gattgaaaat cctaacttca
     8161 ctgttgatat ggaaaaactt ttagaaacta aatctaaagt tgttaatact cttgttggtg
     8221 gtgttgcagg acttcttcgt agctacggag ttactgttca taaaggaatt ggtacaatca
     8281 ctaaagacaa gaacgtcttg gtaaatggct ctgaattgct tgaaaccaag aaaatcattc
     8341 ttgctggtgg ttcaaaagtc agcaagatca acgtccctgg tatggaatct ccacttgtca
     8401 tgactagtga tgacattctt gaaatgaacg aagtgccaga aagccttgtt atcatcggtg
     8461 gtggagttgt cggtatcgaa ctcggtcagg ccttcatgac atttggttca aaagtgactg
     8521 ttatcgaaat gatggactgt atagttccag ctatggatgc ggaagtttct aagaaccttc
     8581 gcttgatcct tgagcgtaaa ggaatgacca tcttgactgg tactaaactg caagaaatca
     8641 ttgaggaaaa tggtcaactt cgtatcaagg ttgaaggaaa agacgatatc atcgcaagca
     8701 aagctcttct ttcaattggt cgtatgccag accttgaagg tattggagag gttgagtttg
     8761 aattggatcg tggttgtatc aaggtcaacg aatacatgga aacttcagtt ccaggcattt
     8821 atgcaccagg tgacatcaac ggtactaaga tgttggctca cgcagctttc cgcatgggtg
     8881 aagtttccgc tgaaaatgcc cttaaaggaa atcatgcagt tgccaaattg aatttgactc
     8941 ctgcagccat ctacactctc cctgaagtag cagcagtagg tttgacagaa gaacaagccc
     9001 gtgagaaata cgatgttgcc atcggtaagt ttaactttgc tgctaacggt cgtgctattg
     9061 catctgacgc tgctcaaggt ttcgtaaaag ttatcgctga taagaaatac ggagaaatcc
     9121 ttggtgtaca cattattggt cctgcagccg cagaattaat aaacgaagca tcaagcatca
     9181 tcgaaatgga aatcactgtt gaggaaatgc tgaagaccat ccacgggcac ccaacttact
     9241 ctgaagtgat gtacgaagcg tttgcagatg ttctaggaat ggccatccat tcacctaaga
     9301 aaaaataaaa cgaagataga ttagactgga aggttatttc cagtttctat catataaaat
     9361 aaagggatat catgaaatac attatcaatc attcaaatga cactgctttt aatattgcct
     9421 tggaagaata cgcctttaaa caccttttgg atgaggatca aatcttccta ctttggatta
     9481 acaagccatc tatcattgtt ggtcgtcacc agaacactat cgaagaaatc aaccgtgatt
     9541 atgttcgcga aaatggtatt gaggtagttc gccgtattag tggtggtgga gctgtttacc
     9601 acgatttaaa taacctcaac tacacgatca tctccaaaga agatgaaaat aaggcatttg
     9661 acttcaagag cttctcaact ccggttatca ataccctggc tcaacttggg gttaaagctg
     9721 agttcacagg tcgtaatgac cttgagattg atggtaagaa gttctgtggc aatgcccaag
     9781 cctatatcaa cggccgtatc atgcaccacg gttgcttgct ctttgacgtt gatttgtcag
     9841 tcctcgcaaa tgcccttaag gtttcaaaag ataaatttga atcaaaaggt gtgaaatccg
     9901 tccgtgcccg tgtaaccaat attatcaatg aattaccaaa aaaaatcaca gtcgaaaaat
     9961 tccgtgattt actcttggaa tacatgaaaa aagagtaccc agagattact gaatacgtct
    10021 tttcagaaga agaattggcc gaaatcaatc acatcaagga tactaagttt ggaacttggg
    10081 actggaacta tggtaaatca cctgaattta acgtccgtcg tggaaccaaa ttcactagtg
    10141 gtaaggttga agtctttgct aacgttactg aatcaaaaat ccaagacatc aagatttatg
    10201 gtgacttctt tggtattgaa gacgttgctg cagtagaaga tgtccttcgt ggggtgaaat
    10261 acgaacgtga agatgttctt aaggcgctaa aaaccattga tatcacacgc tacttcgctg
    10321 gtattagccg tgaagaaatc gctgaagcgg tagttggata aatcaaaaag aagttggcca
    10381 acttcttttt ttgatattta tttacataat ttaaaatacg taaaattata aactatccag
    10441 agcattcttt tgttcatcat taacaatatg ggtatagagg tcagtgactt gtgtgctggc
    10501 atgtcctagt tggtgactga ctaaaacttg tgatttagtc gcatcataga gcctagttgc
    10561 tagtgtatgg cgcagtttat ggggtgttac acgtacttta aaatcctctg agtatttagc
    10621 aaccattttc tcaacgctag aagcatcgat acgattagga acacctctgt agagagttaa
    10681 aaaaagggct gtatctgttt tttccgtttt atagcgttga ttccgaatgg ccagataatt
    10741 ctctaaataa ggtttagcaa aagcagcgac attgactgag tcacgtttgc caccttttcg
    10801 agtaacatca ataaccatca tttttagatt gagatctctt agatctagat taacagcttc
    10861 agataagcga acaccagatg ccaagagaag ggcaataatg gctaaatctc gttctttatt
    10921 tttgttgaat gatgagagag ctcgatttga aagctgttgt gggtgctctt gatcgatata
    10981 agttagaaaa ccttctgttt catcacctag aaagagtttt tgcttgatat tttcagctct
    11041 ggcagcaagg gtttctttct ttttcttggt tgaaactttt ttcattacat tacgatagaa
    11101 ataaggttcc ccctgatcgt tttcaacctc ctcggttaga tacttgtaaa gactagaaag
    11161 tgctgataag gttcgattga tagttgtctg tgaaacaccc tgttttgttg tattagcatt
    11221 cagcaaggga cgttcacgta gataaaggat aaaggattcc atgtctttct tagacatatt
    11281 ttccaaaact gataaaggaa tatcggatat tttatcagcg tttgaaatac cagactccaa
    11341 aacccagctg aaaaatcggt catattcctt aaggtattcg tacagggttg taaaactgta
    11401 gggtacagcc agcttagatt ggtagtattc cagaacatac cagggcatga gttgttttag
    11461 tttgtcgatt cgttccagta aaatctcacg tttcatgtat tttctccata agtaagtata
    11521 ctagtagtat agcatagact tgtatatttt tcaagaaaat tatagttttt cagaaagatg
    11581 ttataggagt ttttcaaagt gataaaaaga cttagaccta caaacaaaac agaataagga
    11641 aaataataaa aatctcaccc actaaacttc tgttaatact acataactga agagctatat
    11701 aaggagctat gagatacaaa acacattaat agtacatctg gacttctaaa acattgctag
    11761 aaattgattt gattgtcctg attgttttat cctatttcta tttca
//
LOCUS       DAVLVZ010000055        11698 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000055 DAVLVZ010000000
VERSION     DAVLVZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.055"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            153..833
                     /locus_tag="VYN77_001569"
     CDS             153..833
                     /locus_tag="VYN77_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1559011.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
     gene            793..1173
                     /locus_tag="VYN77_001570"
     CDS             793..1173
                     /locus_tag="VYN77_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1559012.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            1183..1470
                     /locus_tag="VYN77_001571"
     CDS             1183..1470
                     /locus_tag="VYN77_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1559013.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            1471..2229
                     /locus_tag="VYN77_001572"
     CDS             1471..2229
                     /locus_tag="VYN77_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1559014.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDILLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKAQGRDKKEVTEEALQLLERVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADQIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            2241..3105
                     /locus_tag="VYN77_001573"
                     /pseudo
     CDS             2241..3105
                     /locus_tag="VYN77_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(3185..3832)
                     /locus_tag="VYN77_001574"
     CDS             complement(3185..3832)
                     /locus_tag="VYN77_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559015.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(3829..4527)
                     /locus_tag="VYN77_001575"
     CDS             complement(3829..4527)
                     /locus_tag="VYN77_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559016.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLANWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            complement(4537..5289)
                     /locus_tag="VYN77_001576"
     CDS             complement(4537..5289)
                     /locus_tag="VYN77_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559017.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(5289..6053)
                     /locus_tag="VYN77_001577"
     CDS             complement(5289..6053)
                     /locus_tag="VYN77_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559018.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMVVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(6732..7364)
                     /locus_tag="VYN77_001578"
     CDS             complement(6732..7364)
                     /locus_tag="VYN77_001578"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1559019.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(7398..8099)
                     /gene="pyrF"
                     /locus_tag="VYN77_001579"
     CDS             complement(7398..8099)
                     /gene="pyrF"
                     /locus_tag="VYN77_001579"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1559020.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QLIKQATNPDFICLTPGIRPQGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            8420..8512
                     /locus_tag="VYN77_001580"
                     /pseudo
     CDS             8420..8512
                     /locus_tag="VYN77_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8834..9547)
                     /locus_tag="VYN77_001581"
     CDS             complement(8834..9547)
                     /locus_tag="VYN77_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1559021.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFCKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(9540..10379)
                     /locus_tag="VYN77_001582"
     CDS             complement(9540..10379)
                     /locus_tag="VYN77_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559022.1"
                     /translation="MSMIEVSHLSKSFGDKIALNNISFTVKEGQIFGFLGPSGSGKTT
                     TINILTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMSLYNNLLFYSK
                     FYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPAVLFLDEPTSG
                     LDPTTSRTIHELILELKTAGTTIFLTTHDMNEASLLCDYVALLNKGKLVEQGAPSELI
                     QRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTGGK
                     LNA"
     gene            complement(10855..11664)
                     /locus_tag="VYN77_001583"
     CDS             complement(10855..11664)
                     /locus_tag="VYN77_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEV1559023.1"
                     /translation="MQNKTVLVLGAGALGCYISLSLSMYGVRKLIVADYDIIEPSNLN
                     RQILYTESDVSKEKINVLSEKIHKYNSDVQVVPISIKVSSLEELEKIVAEYGSIDFIV
                     KAIDTPIDIIKIVNQFAVSHKISYISGGFNGCYLIIDNIYIPTIGSCFGCRNINKDIN
                     KYTLSDKTKWPTTPEMPAILGGIMTNLIIKIFLGCYNETLIDNAYVYNMRNHALSQEK
                     YVLENGECPICKKNNKVKDNNIRAKTFIRSVCFCLLSGGVAFYLLLGSSLL"
BASE COUNT         3895 a         2363 c         1866 g         3574 t
ORIGIN      
        1 ttcaaaactt ttcaaatatt ttgtttggaa atttcagaaa ttttatgtct atggtaaaaa
       61 atccttataa cggcaataaa aaatagatat tatccaaaga agattttaag tgctacaata
      121 actgtattat ttctagatgg gaggttctat ttttggattg gtccattgtt gaacaatatc
      181 taccactata tcaaaaggca ttctttctga ccttgcatat tgcagtttgg ggaattttgg
      241 gatcctttct gctcggttta atcgttagta tcatccgaca ttatcgaatc cctgttttgg
      301 cgcaagtagc gacagcctac attgaattgt cacgtaatac gccccttttg attcaactct
      361 tctttctcta cttcggtctt ccccgaatcg ggattgtcct atcttcagaa gtctgtgcaa
      421 cgcttgggct tgtcttttta ggaggctcct atatggcaga atctttccga agtgggctgg
      481 aagccatcag tcaaacccag caggagattg gcctcgctat tggtctgaca cctctacagg
      541 tctttcgcta tgtggttctt ccgcaagcaa cagcggtggc actcccctcc tttagtgcca
      601 atgtcatttt ccttatcaag gaaacctctg ttttctcagc agtggctttg gccgacctca
      661 tgtacgtcgc caaggatttg attggtctct actatgagac agacattgcg ctagctatgt
      721 tggtagttgc ttatctgatc atgctgctac ccatctcact ggtctttagc tggatagaaa
      781 ggaggacccg ccatgcagga ttcgggaatc caagtactct ttcaaggaaa taatctcctg
      841 agaatcttac aggggttggg cgttacgatt gggatatcca tcctgtctgt cctcttatcc
      901 atgatgttcg gaacagtcat gggaatcatc atgacctccc attctagaat catacgattt
      961 ttaacacgat tgtatctgga atttatccgt atcatgcccc agctggtgct actcttcatc
     1021 gtttactttg gcttggctcg aaactttaat atcaatatct caggtgagac ttcagctatt
     1081 atcattttta ccctctgggg aacagctgaa atgggagact tggtacgtgg agctatcact
     1141 tctctcccta aacatcagtt tgaaagtgga taggcactcg acttgactaa tgttcaactt
     1201 tactaccaca tcatcatccc acaagtcttg agaagactgc taccgcaggc tatcaatctt
     1261 gtcactcgga tgattaaaac cacttcatta gttgttttga ttggggttgt ggaagtgacc
     1321 aaagttggac aacaaatcat cgatagcaat cgcctgacca tcccaactgc ttcattttgg
     1381 atttatggaa ccattctagt cttatatttc gcagtttgct accctatttc caaactatcc
     1441 actcacttag aaaaacattg gagaaactaa atgtctgaaa ctatcttaga aatcaaggaa
     1501 ctaaaaaaat ccttcggaga caatcccatc ctccaaggac tttctctaga aatcaaaaaa
     1561 ggggaagttg ttgtcatcct agggccatct ggttgtggga aaagtaccct ccttcgttgc
     1621 ctcaacggct tagaaagtat tcaaggtgga gatattcttc tggatggtca gtctatcgtt
     1681 gaaaataaaa aagattttca cctagttcgc caaaagattg gcatggtctt tcaaagttat
     1741 gaactctttc cccatctgga tgtcttacaa aacctcatcc taggccctat caaagctcaa
     1801 ggaagggaca agaaagaagt aacggaagaa gctttgcaat tactagagcg tgtcggtttg
     1861 ctggataaac aacatagctt tgcccgtcaa ttatctggtg gacagaagca acgtgttgca
     1921 attgtccgtg ccctcctaat gcatccagaa atcatccttt ttgacgaggt gactgcttcg
     1981 ctggatccag aaatggtgcg tgaggtgctg gaacttatca atgatttggc ccaagaaggc
     2041 cgtaccatga ttttagtaac ccacgaaatg cagtttgccc aagccattgc tgaccagatt
     2101 atcttcctcg accaagggaa aatcgctgaa gaaggaacag ctcaagcctt ctttaccaat
     2161 ccgcaaacca aacgagccca ggaattttta aacgtctttg actttagcca attcggctca
     2221 tatctataaa ggagattctt atgaaactat tcaaaccact cttaactgtt ttagcacttg
     2281 cctttgccct tatctttatc actgcttgta gctcaggtgg aaacgctggt tcatcctctg
     2341 gaaaaacaac tgccaaagct cgcactatcg atgaaatcaa aaaaagcggt gaactgcgaa
     2401 tcgccgtgtt tggagataaa aaaccgtttg gctacgttga caatgatggt tcttaccaag
     2461 gctacgctac gatattgaac tagggaacca actagctcaa gaccttggtg tcaaggttaa
     2521 atacatttca gtcgatgctg acaaccgtgc ggaatacttg atttcaaaca aggtagatat
     2581 tactcttgct aactttacag taactgacga acgtaagaaa caagttgatt ttgcccttcc
     2641 atatatgaaa gtttctctgg gtgtcgtatc acctaagact ggtctcatta cagacgtcaa
     2701 acaacttgaa ggtaaaacct taattgtcac aaaaggaacg actgctgaga cttattttga
     2761 aaagaatcat ccagaaatca aactccaaaa atacgaccaa tacagtgact cttaccaagc
     2821 tcttcttgac ggacgtggag atgccttttc aactgacaat acggaagttc tagcttgggc
     2881 gcttgaaaat aaaggatttg aagtaggaat tacttccctc ggtgatcccg ataccattgc
     2941 ggcagcagtt caaaaaggca accaagaatt gctagatttc atcaataaag atattgaaaa
     3001 attagcaagg aaaacttctt ccacaaggcc tatgaaaaaa cacttcaccc aacctacggt
     3061 gacgctgcta aagcagatga cctggttgtt gaaggtggcc attaactctt aaaagggact
     3121 ggattttaag ctccaatccc tttttaagat tttacctata acatcctgag tctatctaag
     3181 atgttcaatc tgaacacagt gtacatactt tatcttctat tgcatatact ttatcacata
     3241 agatacgaat atcctcttca ctgtgactag caatcaaaat tgttgtccct ttttcactag
     3301 agagctttct aaacaatgtt ctcatatttt ctacacttga tttatccaag gcattcatag
     3361 gttcatctag taaaagaata gagggattct ccataattgc ttgagcaatc cctagctttt
     3421 tcctcatacc tagcgaataa gttttaactt tctggtcttt ttgctcatat agaccaacta
     3481 ttttcagtgt atcattgatt tcctgattac caactactcc tcgtatgctt gccaaatatt
     3541 gtaaattctt aaagccacta taataattta taaaaccagg ttcttcaatc aaagctccca
     3601 aattagctgg aatttttctc tcaggaacaa tattttcccc attgattaac acttctccat
     3661 aagacggact atataaacca gctattaatt taaacaatac acttttccct gagccattcg
     3721 caccagtaat tcctataatt tccccctgtt tacaactaaa attaaggttt tgaaaaacac
     3781 atgttttttt taatttcaac tcaatatttt ttaatatgat tatttcattc attctataaa
     3841 cctcctcttt tgacgagtga aatagaaaat acttttaaaa agaaagacta aaaatagcaa
     3901 ctgaagaaat aaatctcgtc ctatatctcc attccctcga ttcaaaatat aaaatagata
     3961 attagttcga tttcctacaa atagaccacc aaacacaatc atgagtaaaa agaaactaac
     4021 gcaagcaaag ttcatttgta cattcgctcc tagtgcgtat aacataaagc aaagcaagga
     4081 tagcacacaa gcattcaaag aatcaactat aaacaaaact cctatatccc agtttgctaa
     4141 ctgtaacata aattgaatca ctagaacttt taccatgatt aaaatgagtc cataaagaaa
     4201 acaggtccaa atctgatacc taatccagag cttaatagaa gaaaatcgaa tcacttccat
     4261 aaagaaatcg gatgctcttc tacgatatac aactgctaga aaaaatgtat gaataataaa
     4321 ccaatgaagc aaccatatag ataagactaa aaaagagttt tgactcagat taggcccttt
     4381 taaaatttct ataacaagtc tatcatttcc ttctaaccaa taccataatc ctaaacaaat
     4441 caatatccaa aaaagaacat aaccccaaaa gtacttggta tagattatct gattcaacct
     4501 ccacatgtga ctacttaact ttcccatttc caacttttac actctcctcc agtttctatg
     4561 aacagaaaat acagtcaatg gaattatgat agatagtaat gtaagccaat aaatcaatgc
     4621 atacggattg gaatttacca tatactgaaa aattggcaat aatccaatta tcccattgtt
     4681 caactgataa atccagaata aggattctac aaataaaaaa gtaaacagaa atactaagct
     4741 catgtttgat tttttaaacc aagaaaaact gctaatagta agtaaggata aaaagaaata
     4801 gtatgctata taagagaaaa aaaatcctat aaagaaacta gcattgtttg caatacttat
     4861 accataaaat tctcttaaaa aatcaacctc ctttatctcc aaagagaagc taaaaccatt
     4921 actaaatgca attagaaaaa tcaataaaaa taaagtcgcc gtccaaatcc ccgtactaag
     4981 agctgctaat ttgaaactaa aactggtaaa gtgcttaatt gatttcagac gaatgcgaca
     5041 ctccaaccta ttaaaatagt tattcatcaa ataaaaaaag aacaatatat atgtgaacgg
     5101 aaagcaatat actccagtcg tcatatcttg aagtaaaact aaaatccatt ctaatacatt
     5161 tggatggatt gaatattggc gacagcgcaa taaatatact gtactagata aaacacagga
     5221 tagcagtaat ataaaataaa ccaatactgc taagaaatct tttttataaa attgaacaaa
     5281 ttgtttcatt atacatagtc ctctgaatgt agaaaaaatg taccataaac aaccaaacaa
     5341 ctaacaaata aaataaaagc aagatgccca ctaactaagg aaagactgat atctttctga
     5401 tatcccaaag ctagcgttgt cacaggttct aagtaagata gccctaaaat agcccataaa
     5461 ataccaccaa ccaccatata ggcaactggg atgaaaatag ctcctaattt tttcttcact
     5521 agcaaagcac tagctagtcc aaaaatagag aacacagcgc cccaaactcc ataccagaga
     5581 gtcgtcacaa gactatagag caactgatta gaatcaaata attcttttaa ggcaccacta
     5641 taatctccaa tataaatttc ctgataagga gtcactaaaa gattaattcc taataataat
     5701 aaatagggga gaaaaaagac tagaaaagaa gaaataattg cagtactacc tacaatagct
     5761 agatacctct ttttagaaat tcggcacaat tgtgctgtta gaaaatgact ctcagcatcc
     5821 tctattatct gactagaata gggcagtgta cagataagtg cagctactag gctaatcggt
     5881 gaaaatcctc gaatagaaga agcggcaaaa aaaatcgata aaccttcaat tttataaata
     5941 ccattgaaag caaggaaatt tcctaaactc atgcaaagaa gggtcaaaaa taaagacata
     6001 taaaaccgag gtgattgaac gactcgtaca agattacaaa tgaaaaattc catccttact
     6061 cctccttata ataaaaatag ggtgtagcat tcttttttca tgctacaccc acaatcaacc
     6121 atctttaagg cttactctga caagtaagtt aataagaatc tggactccaa gaacctgaag
     6181 tatgaattct tacatgattt ccaaattgtg gcgccatagc taatctagta ccagaaccaa
     6241 tataattgtc accacctcca ttatagtaca tgacaatcct agagccagac cccaatgaat
     6301 ataccggggt aatatctgac ccactatagg cgctacgaat agaggtattt aaccttttac
     6361 cgccaccagt gctgtcacta ttattaattc cagcagaggc gttttctttc tcaagctcac
     6421 tagttgtatc cgaaccccaa cgcggcaacg ttaaatcata atttccaatc tgtctagcta
     6481 aaactgtagg gagaaacaaa ctagatgcaa ttgtaactac gcaaaaaagg aatatatttt
     6541 tcttcttttc atgacatcgt cctcttaaga agtgataaac aagatagttg attgatcaat
     6601 ttgatacata ttacattttc attatatcaa taaaagaaag cgttgtcaat tattttattt
     6661 aaaatattat catattttaa ataaaatggt aaaagagact cggtagcctc gtctctcttt
     6721 atcttactga cctaaccttc ttgccaattt tcttggtctt ctttaaagcg ttttagaaga
     6781 tcaaggccct ctggcgtgat gtaaccttct tcttgggcta gatggataag ctcgctatag
     6841 tttgaaagcg tcacaagttt aacaccagca tctgcaaagt tcttatctgc ttttggcaat
     6901 tggtagctga aaatcgctac aactccaagt acatctgctc cttctcgctt ggctgctgct
     6961 acagcttcaa gaactgaacc acccgttgaa ataaggtctt caaccactac cattttttga
     7021 ccttgagcta cgcgaccttc gatttgatta ccagctccgt ggtcttttgg tttgctacgg
     7081 atgtaggcaa aaggcaagtc catcttatca gcaataatgg ctccgtgtgg aatccctgct
     7141 gttgcagttc ctgcaatcac ttctacttca ggaaaggctt ctttgatagc ttccacaaaa
     7201 ccattttcaa ttagggtacg agtttctgga taggctagtg tcacacgatt atcagtgtaa
     7261 atcggtgact tgataccaga cgcccaagtg aagggttcct ctggtttgag gtaaacggct
     7321 tggattttca agaggtggct agcgatatct ttagcaagtg tcatggtatt ctccttttat
     7381 ttttctaatc tagttcttta attccagtcc tgtgtccatt catccttgat ggcatgataa
     7441 gctgcaacag gatcctcagc ttgggtaatg ggacgtccca ctacgatata gtcactacca
     7501 atttgataag catctgctgg cgtcatgacg cgtttttggt ctccaacagc agctccctgt
     7561 gggcgaatcc ctggtgtcag acagataaaa tctggattgg tggcctgctt gataagttga
     7621 acttcctgtg ccgagcaaac gacaccatcc aagcctgctt cagctgtctt cttggcatag
     7681 tggatcacag actcttgtag gcttgtttgg atattttgaa actcctgcat ctgggcttct
     7741 gatgttgatg tcagctgcgt tacagcgatc aattttgctt gacttccaag accttcacgc
     7801 gcagccttca tcatctctac accaccagcc gcatgaacat tggtcatatc gacaccaagc
     7861 tgagacaaga ccttcatggc tgacttgact gtattaggaa tgtcatgaag tttgagatcc
     7921 aaaaagacac tatgacccaa accttttaag taggacacaa tctcaggccc cgctgcgtaa
     7981 taaagctcca tccctacctt gagataaagg ctttcttctg ctgggaaaag agctaaaaat
     8041 tccttgaccg cctcaaaact aggaaaatca agagcaatga ttggacgatg ttctcgcata
     8101 ggtttctcct tttaccttta ctagtgagag agtctggcca acagaagcca cgaaaaaagg
     8161 aacctgccaa cagcaaggtt ccacgaaaaa tgggtagtct ccaccctttc accgtctaac
     8221 cttagctgcc tctctggact gctttaaagg ttttctatta ttctattatt tctactagca
     8281 cttgtcaagt aaaaacatgg tcacgtatgt gttgactgct attgaacacc tcacaagact
     8341 ctttccaaaa gaatagttaa agactttcac tgaggacaga gaaaaagagt ttgcttgtta
     8401 ccctttaatt gaacaaatgg gaattctgtt ttattttgcg gatgcctatt catcttggca
     8461 aaggggaagt aatgagaatg cgaatggact tcttcgtgag tattttctta aaccgatttg
     8521 gcaacaatta ccgaaaaaat ccttaatggt gctttgctag cttattaatc accgttccca
     8581 aaaatacttg gattacaaga caggttttga agcacttata gaggagattt aaaaatgttg
     8641 cattttttat tgcaatttat cagttatctt atcacccaaa acttctgcaa ttagtatgag
     8701 cactattgtt atttttcttt tttcgtacac tcaaatcaaa atatagcaat aagactattc
     8761 tgtagaattg ctgtcaaaaa gatcagttca tttttccttc ccacttgtgt ttataatcat
     8821 taaaataact caattaagaa attttctttt tcctaatagt tatcgtaatt aaagttaata
     8881 gaagaacaat ccaaataagt agtgttagat taggaattag agtttcattc catgaaaatc
     8941 cctcccattt tgtgaaaaac ttagtaaaca gtcccataaa actataatct gttatcttcg
     9001 caactgtctt atccaaacca gatagcatcg gtaagaaagc aactaaaatc atagcaggaa
     9061 gactgataac ctgagctact acttggctct tcgcggttaa acctatcaat aaatagaata
     9121 aaatgatgga taaagaggtt agaagaacga ttgtaatata attaaaagta tgtacaattg
     9181 taactcctaa aataagagga gtagttccca taatcacaaa agttagcaaa aaaggaagaa
     9241 acatcgttga taaaatgtat tcggatcctt taacaccact caacagaaga gtttgtaaat
     9301 tgtacttttc tttttcttca gacaagataa tagttatagg acttccaaca gccaaagcca
     9361 aagaaaaagg taaacacatc atcaaaagaa ctaatgcctg ttgatcgttg accttcccct
     9421 gtgtttccat aagatattta caaaaatatg tgaaagcaaa aggcactaaa acttgcaata
     9481 aaatactctt attggaaagg ataatttgac aacgcaacca taccaaagcc agaaaccgtt
     9541 taagcattta gctttcctcc tgttaatgtg ataaaaatat cttctaaagt aggctcacat
     9601 gaatgaattg aaaaaatatt ttccagatca gcctgagata cctgttctag tgatgtaaaa
     9661 tcaaaagtta tctgattccc attataatct gtaaccttaa tctttttatc tttattatat
     9721 ctttgaatga gttcagaagg agctccttgc tcaactaatt tccctttatt taataaggca
     9781 acataatcac ataaaagact tgcttcattc atatcatgag tcgttagaaa aatcgttgtc
     9841 cctgctgttt tcaattctaa aattaactca tgaattgttc gagaagttgt gggatctaga
     9901 cctgaggtcg gttcatccag aaagagtaca gcgggtttgt tgataagagc tcgtgctaaa
     9961 agcattcgtt gcctcattcc agtggataat tttcctgcta ccatcttgcg actatcatat
    10021 aatcctactc gctttaacaa attatcaaca cgtgatttac taatattata aaatttacta
    10081 taaaaaagaa gattgttata cagagacatt ttctcataaa atccacttgt atcgctaacc
    10141 aatccaattc tctttaattc accgcttgtt aaattttgag atttttgtcc caaaataata
    10201 gattgtcctt tatcggcaag gaactgccca gtcagaatat taatcgttgt ggtttttcca
    10261 gaaccagatg gtcctaaaaa tccaaaaatc tgaccttctt taacagtgaa gcttatatta
    10321 tttaaagcta ttttatcacc aaaacttttt gataaatggc taacttcaat catactcatt
    10381 gcacctcctt tatattctac atttttactt aacatctatt tacttagctt tatcaaataa
    10441 acgtcttaaa aaaaacaata acattaagca tataaccgtt agcaagattg tttcaatatt
    10501 attgataata tgatacattc tcaagcagta taaaataatt atatatatgc aaatgctcat
    10561 tatacctatt tttttaaaca tactaagcct ccttgccaaa taataaagtt atataataac
    10621 taagacacac tatacctaac ataataaata tccacagcat taaaaatatt atcatacaaa
    10681 taaaaatatc cactggtaac tgaataaatg ttctaaaatt aacaagcaaa aataaaatct
    10741 caaacgatga aaaaagccat actatatttt ccaatgatgt ttgaatattt ttattgtagt
    10801 aagcataata tatagcaaaa ataataccca agaacaatac tattaattgt gtttctataa
    10861 cagtgaactg cccaatagca gataaaaagc tactcctcct gataataagc aaaaacaaac
    10921 tgaacgaata aatgttttcg ctctaatatt gttatctttc actttattat tttttttaca
    10981 aattggacat tctccgtttt ccagaacata tttttcttga cttagagcat gatttctcat
    11041 attataaacg taagcgttat ctattagggt ttcattataa catcccagaa atattttaat
    11101 tattaaatta gtcattatcc ctcccaaaat agcaggcatc tctggtgtag tcggccactt
    11161 tgtcttatca gataaagtgt acttatttat atctttgttt atattccgac aaccaaagca
    11221 agaaccgatg gtagggatat atatattatc aataataaga tagcatccat taaaccctcc
    11281 tgatatgtag gatatcttat gcgatacagc aaattgattg acaattttta taatatcaat
    11341 gggcgtatca attgctttaa cgataaaatc tatactccca tattccgcaa caattttttc
    11401 taattcttct aatgaagaaa ctttaataga aataggtact acctgaacat ctgaattata
    11461 cttgtgtatt ttttcagaaa gaacattaat cttctcctta ctaacatccg actctgtata
    11521 aagaatttgc ctatttaaat ttgatggttc tattatatcg taatcagcga caataagttt
    11581 cctcactcca tacatactta gacttaaaga tatataacat cctaaggctc ctgctcctaa
    11641 tactaatact gttttattct gtattttatt atactcttca ttataaaata acgaatag
//
LOCUS       DAVLVZ010000056        11346 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000056 DAVLVZ010000000
VERSION     DAVLVZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.056"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..1671
                     /locus_tag="VYN77_001584"
     CDS             <1..1671
                     /locus_tag="VYN77_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1559024.1"
                     /translation="EKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGEY
                     EDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQDY
                     GVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNGGFA
                     PYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKNP
                     LFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVSD
                     EVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTK
                     YKDFYYYGYKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINM
                     KVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYD
                     KIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISE
                     IQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            1939..2505
                     /locus_tag="VYN77_001585"
     CDS             1939..2505
                     /locus_tag="VYN77_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559025.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            2827..3015
                     /locus_tag="VYN77_001586"
     CDS             2827..3015
                     /locus_tag="VYN77_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1559026.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3077..3835
                     /locus_tag="VYN77_001587"
     CDS             3077..3835
                     /locus_tag="VYN77_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1559027.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            3893..4384
                     /locus_tag="VYN77_001588"
     CDS             3893..4384
                     /locus_tag="VYN77_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1559028.1"
                     /translation="MLGVIIGDIVGSVYEWNKIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYE"
     gene            4388..4747
                     /locus_tag="VYN77_001589"
     CDS             4388..4747
                     /locus_tag="VYN77_001589"
                     /inference="COORDINATES: protein motif:HMM:NF015692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1559029.1"
                     /translation="MNDNPIECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQET
                     VPQAIIAFLESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLD
                     EPLKDVVRRWENRIKAY"
     gene            4859..5743
                     /locus_tag="VYN77_001590"
     CDS             4859..5743
                     /locus_tag="VYN77_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1559030.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASRERYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKFLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            5906..7645
                     /locus_tag="VYN77_001591"
                     /pseudo
     CDS             5906..7645
                     /locus_tag="VYN77_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            7920..8375
                     /locus_tag="VYN77_001592"
     CDS             7920..8375
                     /locus_tag="VYN77_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559031.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            8820..9296
                     /locus_tag="VYN77_001593"
     CDS             8820..9296
                     /locus_tag="VYN77_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1559032.1"
                     /translation="MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            9296..10072
                     /locus_tag="VYN77_001594"
     CDS             9296..10072
                     /locus_tag="VYN77_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1559033.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQSIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQENGKDSVEYTKDFAGKMVESLV
                     TELSHLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYAINPNPARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAAEVLQEYLFGKWSKVEEEMLKVGEERMRDMSKSNGKDA"
     gene            10056..10865
                     /locus_tag="VYN77_001595"
     CDS             10056..10865
                     /locus_tag="VYN77_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HEV1559034.1"
                     /translation="MEKMHSYIKYLTQEDPIITDEGKQIEILHLDIQDDPEIFEDWAK
                     QFRRNYCSDDELAEMIGFMNISPKEYLENFKLPSDSGIGLSTMSGDFGEILVSDYLQY
                     IEEYVVPRTRYDSKVNKNTSTQGSDVLGYKQDSSNVSNDEVVVIEVKSSASDSSSSKA
                     KNKLQEAINHSDKDFERFSTSIVASYLRLKKSNSDQANVVERFLNITDKPFNVIYGAV
                     AVYSNQSYDIDVIKTVVSKNHRDFQKLRLLVIHSNELMKFIRELYSKASEV"
     gene            10862..>11346
                     /locus_tag="VYN77_001596"
     CDS             10862..>11346
                     /locus_tag="VYN77_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence associated protein"
                     /protein_id="HEV1559035.1"
                     /translation="MIEFGRRSLYFSKLVRSKAKMIEFDIPLESHLPISEDAQKSFLG
                     ALAIVADTARKYFEVYINRKSFNLQLKNQLHNAAEYFDALLVSGLGNSAEYQDYIAIL
                     GTTAYYLCDYNGSSRVMINYISDDIQLLEDCMTLIKVFIDVVTDELFLNHTPIEGKYS
                     S"
BASE COUNT         3988 a         1576 c         2362 g         3420 t
ORIGIN      
        1 gaaaaaataa aagtatacct ctatacacga gtatctacgt caatacagat agaggggtat
       61 tcgttagagg cacaaaaatc acgaatgaaa gcttttgcta tttacaacga ttatgaaatt
      121 gttggagagt atgaagatgc cgggaagtct ggcaagtcta ttgaaggtag aatacagttc
      181 aatcgaatga tggaagatat aaaatctgga aaagatggag tatcctttgt tcttgtgttt
      241 aagttatcaa gatttgcaag aaatgccgct gatgttcttt ctacacttca gataatgcag
      301 gattatggag ttaatcttat ctgcgttgaa gatggcattg attcatctaa agatgctggg
      361 aaactaatga tttctgtttt atcagctgtg gctgaaatag aaagagaaaa catccgtatt
      421 caaacaatgg aaggtcgcat tcaaaaagca agggaaggaa aatggaatgg tggttttgct
      481 ccgtatggat ataaacttga agacggcaag ctgtttataa atgaggaaga ggcagttgca
      541 ataagaacga ttttcgacca gtatgtaaat actacgatag gagccaatgg gatatctaaa
      601 tacttagaga atcatggaat tagaaaaatc ccaagacaga atggtaagaa tcctttgttt
      661 gatgcaggtc ttataagaaa gatattaaag aatcctgtat ataatgggaa aatagcattt
      721 ggaagaagaa ctttagaaaa agttcatggt acaagaaatg aatataagca ggttgaacaa
      781 gatgaatatc taatatctga agggatacat gaagctatag tttccgatga agtttggcaa
      841 gctgctcagg ttaagctaaa atctcaagca aagaaatatg agcatgtgaa taaaggaaaa
      901 gatacacgca cacacttgct ttctggaatt gtaaaatgcc cgatatgtgg agtgggaatg
      961 tttgggaaca agtgtatcaa gaaaaagaaa gatggcacaa agtataaaga tttttattac
     1021 tatggttata aacataggca gatgataaga ggtcataagt gtacattcag taagcaaatt
     1081 agagaagaat tgttagatga tgctgttgca gaggtgattg tcaagatagt aagtaatccg
     1141 aaatttgctt ctatgatgca agaaaaaatc aacatgaagg tggatacctc tgaaatagaa
     1201 aaagaaatag ataattacca aaaggaattg agaaagagtc attctacaaa gtttaagcta
     1261 attgaggaaa tagataattt agatgttgaa gataagcatt ataagagaag gaaacaggat
     1321 ttagatgata gactatatcg tatgtatgac aaaatagatg aattagaatc atcactaatt
     1381 gatgcgaaag caaagaaaca gactattgaa gctgaaaagc ttacaggaga taacatatat
     1441 aaggttctga tctattttga taagctctat aaagtcatga atgatgtaga gcgtaggcag
     1501 ttaatttcag ctttgatttc tgaaattcaa gtttatgaag aaaaacaatc taacggacaa
     1561 tggctaaaat ccattacttt taagctacca attatagaag aaaatctcaa cataggtttg
     1621 gacaatgatg aacaagttga atgtgtaagt ttattagaga aacgtagtta atcctcaaaa
     1681 ggttccccaa accttttgag tcccactgac gcatcacgct gaggcggtat cactgcttgt
     1741 acgagttgaa gcatcagcga agtaaaagta gatgttcagc caatgcgacg aggtgagacc
     1801 gaaggtcgat aaggtgtgtt ctgggtatag gactcgtaaa gtaagtagga aatatgatga
     1861 gatgatacag agagcctgaa ttggtcaggt gacgtgctta tttacattag cctgataatg
     1921 aaaatggaga gattatcgat ggttatattg gaattgtacc aaaatgatta ttctaaagat
     1981 ttagtagcat ttgattccat agaagatggg aaagcctttg tagctcaaat cccgggatac
     2041 actctggaaa cagaagatgg ttttgaagtg gagtatttta atcctaaaaa cataccggat
     2101 tatatggaaa ttatttttaa cggaaatatt gttcctttat ctaaatttat gttcgatcct
     2161 gaggaaaatg taaacattat ttggaaagag atttcaaatt tatccctcaa aaatgataga
     2221 gtgatcgaag gatattcaaa aattgatgca tatgtcgtta acaaccatga agttaaagcc
     2281 tatgtcgaaa caagagaaac gaactatcgt aaagcaaaag acttcttaga aagccgcgga
     2341 tatgaaatcg acagaagctt tttcggaagt gaagacggtg aagcgattct ttacagaaaa
     2401 aaagggatcg aagtttggca tttcttgtgt cacttagacc caatgtttgt agagattgaa
     2461 gatgtagaag gatatgttaa agaagaagtt ggagagattc aatagtcaac caaagttcct
     2521 tgtgagtata tacctttgct agtaaaagag tcaaaacgtt ataacgaagg gggggaagaa
     2581 aacccttgag tgtaagtctt tttcttgttt agagggaaag tattagagta gccgctcata
     2641 atttatacta gatttgataa ctgatttgag acagatttta tttctataaa tattgtgaaa
     2701 attttttaat accaaagatt taaagttact gtgttaaaaa tcctctccac ttttaaacta
     2761 atgtattata aaagccagtt gacactagtg ttacacctgt gatagaatta acttgaggtg
     2821 atgaaaatgg caaccgataa aaatagaata atgattagct tggatgataa aaatctagag
     2881 aaacttgaaa atttagtaga agatgctaga gatagaaggg gaatgcgact aacaaagtcc
     2941 caggttattg aattactcct aaataccgtt gattatttcg atgatattat gggggctatt
     3001 tactcaaaaa aataagagca aaattgctat tattttttac aaattggtgt aacactagtg
     3061 atataggagt gatgaaatgg ttgtttatat tagacaaagt aaattaccaa gtgaggtttc
     3121 aattaataaa tacaatgctc aagtaggtgc ctacttacaa ggagaagaag ttattttata
     3181 tcaatctttt tctgaaataa aagagttaac aagtgaagat atagttgtag attatataat
     3241 ggagactaga gcattactaa aaatgatggg cttaaacgtt ccggttcatg actatcctat
     3301 tgagcttaaa gagttttatg gtcgaaagat ttatgctggt attttaggag agattgtgaa
     3361 tatacctgat aattggggaa aatttattaa gcctaaagct ggttcaaaag tctttactgg
     3421 aagagttgtt aatggaaccc atgatttaat aggtattggt ctacctttcg actatcctat
     3481 atggattagt gaggttgtag aattcatagc tgaatggcgt tgttttgtgt tagatggtcg
     3541 cgtattagat gttcgaccct atacaggtga ttatcatgca caatttgatg caagtgtaat
     3601 tgatgaagcg atatcatgtt ggaaagatgc gccaatagct tatggactag atatcggtgt
     3661 tactcgcgat ggcagaacac ttgttgttga agtaaatgat ggttatgcat tgggaaatta
     3721 tggcctatct cctttaaaat caatcaattt tcatagagct aggtggaaag aaatggtaaa
     3781 accctatttt gaaaaaaatg aaatttttaa aatccaacag gatgttattt tctaaaataa
     3841 atatggtaga taaccaatat aaaaagcatc aaaatgaaca aggaggaatc tgatgcttgg
     3901 agtaattatt ggagatattg tcggttctgt ttacgaatgg aacaaaatca aaacaaagga
     3961 ctttccttta tttcggaagg actgcttttt tacagatgac acggttatga cctgtgctgt
     4021 tgcagaagca attatgaatg gtggacaaaa agacgacttc attgacgcga tgaagaaata
     4081 tggcagaatg tatccgaatg ctgattacgg tgctcggttt aatgcatggc taaacagcga
     4141 taaccgtgag ccttataata gctttgggaa tggatcagct atgcgtattt ctccatgtgc
     4201 ttggatcatg gactgtggtt tttatgcgaa aactggtata tggccatcat ctagaggact
     4261 tacgagtctt tctgcagagg taactcataa tcatccagaa ggtgtcaaag gcgctatggc
     4321 tacagctgat gctatctttc tatgtcgttt ttactttggt ggttattgta gagagtacga
     4381 gtaatcaatt aacgacaatc ctatagaatg taaaagacga attaaggatt atatagaaaa
     4441 agaatacgat tacaatctat ctcaaacttt agatgaaatc cgtcctaact atcgttttaa
     4501 cgaaacatgt caggaaactg tccctcaagc cattatcgcc tttcttgaaa gtagagactt
     4561 cgaagatgcg ataagaaatg ccatctcact tggtggcgac agtgatactc tcgcagcaat
     4621 cacttgtagc atagcagagg cagcttacgg tattcctgat tggatcaagg ataaggccta
     4681 ttcttacttg gatgaaccct taaaggatgt agttaggcga tgggaaaata gaataaaagc
     4741 gtattaatct ccagtacgtc agatttctct tttctgtatt ttcattgtat aataggatta
     4801 catttacttg catacgacga aatgtaaaca tttctattta aaattaagga taaaatcaat
     4861 gaaaaaagca atggtaatta tcaaccctac ttctggtggc gagaaggctt tggattacaa
     4921 agaaaagctg gagaataaag caaaagaata ctttgaatat gttgaaacca aaattaccga
     4981 aaaagcgctg gatgcaacac attttgctga agaagcttct cgtgagcggt atgatgcagt
     5041 ggttgtgttt ggtggagatg gaactgtcaa tgaagtcatt tcaggtattg atgagagaga
     5101 ctacattcct aagttaggga ttatcccagg cggtacgggt aacctcatta caaaattttt
     5161 ggaaatcaat caagacatcg atggcgcaat tgacgaactg gattttgatt taaccaataa
     5221 gattgatatc ggtaaagcaa atgacaacta ttttggttat atctttagta tcggttctct
     5281 gcctgaggcg attcacaatg ttgaaataga ggacaaaaca aaattcggta ttttaaccta
     5341 tgctgtaaat accatgaagt ctgtcatgac agatcaggtc tttaacatta aggttgagac
     5401 agaaaatgga aattatgttg gtgaagctag ccatgttttg gttcttttga caaattactt
     5461 cgctgataag aaaatctttg aagaaaacaa ggacggctat gccaacattt tgattctgaa
     5521 agatgcctct atattctcca aattatccgt cattcctgat ttattaaaag gggatgttgt
     5581 cgcaaatgat aatatcgagt atatcaaagc gcgtaatatt aaaatctctt cagatagtga
     5641 attggagtca gatgttgacg gagataaatc agataaccta cctgtagaaa tcaaagtcct
     5701 agctcagcga gtagaagtat tttcaaaacc gaaagaggat tagtatatag agaaagcctt
     5761 ttttaaggct ttttgtatac tttaaaagat agttccttta acaacggaca ttccttgcaa
     5821 atagtttaca aaaatagtat actggattta ttgagtttga aaacgtttgc gtaaaatttg
     5881 aatgaatact ttaggagaca aattgatgga attaagtgct atttaccata ggcctgagtc
     5941 ggagtatgcc tatctttata aggataagaa actccatatt cgaattcgaa ctaagaaagg
     6001 ggacattgaa agcatcaact tgcactatag ggaccctttt atctttatgg aggagtttta
     6061 tcaggataca aaagaaatgg tcaagataac ttctggtacc ttatttgacc attggcaggt
     6121 tgaagtgtca gttgactttg cacgtatcca gtatctcttt gagctcagag atacagaagg
     6181 tcaaaatatt ttgtatggcg ataaagggtg tgtggaaaat tctctagaaa atcttcatgc
     6241 aattgggaat ggatttaagt tgccttatct tcatgagatt gatgcctgca aggttcctga
     6301 ctgggtttca aatacggtat ggtatcagat atttcctgaa agatttgcca atggcaatgc
     6361 tctattaaac ccagaaggga ctttagactg ggattcatct gtcacaccta agagcgatga
     6421 tttctttggt ggtgatttac aggggattat tgatcatatg gattacttgc aagacttggg
     6481 tattactgga ctatatcttt gtcccatctt tgaatctaca agcaatcaca agtacaatac
     6541 gacagattac tttgaaattg accgtcattt tggagacaag gagacctttc gggaactggt
     6601 ggatcaagcg catcatcgtg gcatgaaagt catgctggat gcggtattta atcatattgg
     6661 ttcgcaatct cttcaatgga aaaatgtcgt caaaaatggt gaacagtctg cttataagga
     6721 ttggttccat attcaacaat tcccagtgac aactgaaaag ctagttaata agagagactt
     6781 accctatcat gtttttggtt tcgaggacta tatgcctaag ctaaatacag ccaatccaga
     6841 ggtcaagaat tatcttttaa aggttgcgac ttattggatt gaagagttta atatcgatgc
     6901 ttggcgtttg gatgtggcta gtgagattga ccatcagttc tggaaggatt ttcgtaaggc
     6961 agttttagct aaaaatcctg atctttatat cctaggagaa gtctggcata catctcagcc
     7021 ttggctaaat ggagatgagt tccatgccgt catgaattat cctttatctg atagtatcaa
     7081 ggactatttc ttacgaggaa ttaagaagac agaccagttc atcgatgaaa tcaattgaga
     7141 gtctatgtat tacaagcagc agatttcaga ggtcatgttt aatctcttgg attcacatga
     7201 tacagagcga atcctgtgga cggccaatga agatgttcaa ctggttaaat cagccttagc
     7261 ctttctcttt taacaaaaag gaacaccgtg catttattac ggaaccgagc tagccttgac
     7321 tggagaacca gatccagatt gtcgtcgttg tatgccttgg gaacgtgtat caagtgacaa
     7381 tgatatgctg aactttatga agaggctgat taagattcgg aaatacgcgt cagtaatcat
     7441 ttcgcatggc aagtatagcc ttcaagaaat caactctgat ctagtagctc tggaatggaa
     7501 atacgaagga cggatcctca aagcaatatt caaccaatca acagaagatt atcttttaga
     7561 gaaagaagca gtagcactag caagcaattg ccaagaattg gagaatcagc ttgtcatctc
     7621 tccagatgga tttgtgattt tctaaaaact agttgatgaa gattatggta catttcatat
     7681 cttatatagt ataataaggc tagttactaa acttgtaaag gagaacttaa atgaattgta
     7741 gaggacatga aacaagacaa agaattgtta gagattttga agtttagcct aaagcacata
     7801 ttaagctgtt agcaaatcaa caaaaacata gtgatgcagg agcaactatt gaagatgaat
     7861 attatgtatt tatcgctgag agtaaaattg atggcaagaa ggaagttatt cagtgttgca
     7921 tgggtgcggc aagggatttt ttagaactaa ttaatcacaa agggctacct ctttttaatc
     7981 cgcttgtagg tgattctcat gtaaataata gacaagaata tgacaataca gggagtggaa
     8041 atttacaacc tgaaaagtgg aatgaaactg caaagcagct ttataatgtt ataatgtggt
     8101 tgattatttt atggaatgct aagccggata cacctttatt taattttaaa gacgaagtaa
     8161 ttaagtataa aacatatgag ccttttgaaa gcagtataaa aagagtaaat actactataa
     8221 agaatggtag taaagggaaa actctgactg agatgattaa tggctacaga gcggataacg
     8281 atattagaga tgaaatttgt aactttaata ttctgaaaaa taaaattcgt gatatgaaag
     8341 accaacaagg aaatacaatg gaatcttact tttagttatt gttgaatttt gggtattcta
     8401 taaaatatcc taattgagat ttaaatagta gactatacaa tatagttaaa atatcagtaa
     8461 aaacaacact ttattgaggt attggatacg ctttgctaat agcctaataa tcacatgtgg
     8521 agtgttgcta caacgaaaaa ggtgataatc cttgatttca agctatttta taagcatttt
     8581 gtctttgtag ataaaggcaa ttttgacaat aaaaatccta aaaggtgaat cgttatagat
     8641 gtatttgtag atatcgtttg cgcatcgaaa aaattaatac aagaataaat atttatggct
     8701 ctttaggtga cttttataga agtaaagttt aggatagaaa aacagtcaat aacgcaccat
     8761 ttttggtgcg ttatgctttt ttatgctata atggatttat aaaaataaag gagtttgcca
     8821 tgattggaaa aaacataaaa tccttacgta aaacacatga cttaacacaa cacgaatttg
     8881 cacgaattat aggaatttct cgaaatagct tgagtcgtta tgaaaatgga actagttcag
     8941 tctctacgga attaattgac atcatttgtc agaagtttaa tgtatcttat gtcgatattg
     9001 taggagaaga taaaatgctc aatcctgttg aagattatga attgactttg aaaattgaaa
     9061 ttgtgaaaga aagaggtgct aatctattat ctcgactcta tcgttatcaa gatagtcagg
     9121 gaattagcat tgatgatgaa tctaatcctt ggattttaat gagtgatgat ctatctgact
     9181 tgattcagac aaatatctat ttagtagaaa attttgatga aatagagaga tatagcggct
     9241 atttggatgg aattgaacgt atgttagaga tatctgaaaa gcggatggta gcttaatgga
     9301 aatccaagat tatactgata gtgaattcaa acatgcttta gcaaggaatc ttcgttcact
     9361 gacaagagga aaaaagtcca gtaaacaatc tatagcgatt ttgcttggag gtcaaagtgg
     9421 tgccggtaag actacaattc atcgtattaa acagaaagaa tttcaaggaa atattgttat
     9481 catagatggt gatagttttc gttctcagca tccacactat ttagaactgc agcaagaaaa
     9541 tggcaaagat agcgttgaat acaccaaaga ttttgcaggg aaaatggtag agtctttagt
     9601 aaccgaattg agtcatttgg gatacaatct tttgatagag ggaactttac gaactattga
     9661 tgttccaaag aaaacagcac aactattaaa aaataagggg tatgaggtac aattagcctt
     9721 aattgcaacc aagcccgaat tgtcctatct gagcaccctt atccgatacg aagaactgta
     9781 tgctatcaat ccaaatccag cacgcgcaac tccaaaagaa catcatgatt tcattgtaaa
     9841 tcatctagtt gataatacac gacaattgga agaactagct atttttgaaa gaattcaaat
     9901 ttaccaacga gatagaagtt gtgtatatga ttcaaaagaa aatacaactt cagcagcaga
     9961 agtgctacaa gaatacctct tcggaaaatg gagcaaggta gaggaagaga tgttgaaggt
    10021 gggagaagaa cggatgaggg atatgagtaa aagtaatgga aaagatgcat agttatataa
    10081 agtatctaac tcaagaagat cctattatta ctgatgaagg taagcagatt gagattcttc
    10141 acttagatat tcaagatgac ccagaaattt ttgaagattg ggcaaaacaa ttcagaagaa
    10201 attattgttc tgatgatgaa cttgctgaaa tgataggttt tatgaatatc tcccctaaag
    10261 agtatcttga aaattttaaa ttaccctcgg atagtggtat tggcctttca acgatgtcag
    10321 gagattttgg agaaatatta gtatctgatt atcttcagta tattgaagaa tatgtggttc
    10381 ctaggactcg ttatgatagt aaagtcaata aaaatacttc aactcaagga agtgatgttc
    10441 taggatataa gcaagattct tcgaatgtgt cgaatgatga ggttgtagtt attgaagtaa
    10501 aatcatctgc atcagattct agtagctcta aagcgaaaaa taagcttcaa gaagctatta
    10561 atcattctga taaagatttt gaacgatttt caacctcaat tgtagccagc tatttgaggt
    10621 taaagaaatc taatagtgat caagcaaatg tagtagaaag atttttaaac ataacggata
    10681 aaccgtttaa tgtaatctat ggagctgttg cagtctactc caatcaatca tatgatattg
    10741 atgttatcaa gacagtagtt tcaaaaaatc acagagattt tcagaaatta cgactattgg
    10801 tcattcattc taatgaatta atgaaattta ttagagaact ctattcgaaa gcgagtgagg
    10861 tatgatagaa tttgggagaa ggtcattata tttttcaaaa ttagtgcgtt caaaggcaaa
    10921 gatgattgaa tttgatattc ctttagaaag tcatttacca atttcagagg atgctcaaaa
    10981 atcgtttctt ggggcactag caattgttgc agatactgct aggaagtatt ttgaagttta
    11041 tatcaatcgt aagagtttta atttacaatt aaaaaatcaa ttacacaatg ctgctgaata
    11101 ttttgatgca ttgctggtgt caggattagg aaattcagct gaatatcaag actatattgc
    11161 aatattgggt accacagctt attatctatg cgattataat ggtagttcga gagttatgat
    11221 taactatata tcggatgata tccagttatt agaagactgt atgactctta taaaagtatt
    11281 tattgacgtt gtaacggatg aactcttttt gaatcatacg ccaatagaag gtaaatactc
    11341 tagtga
//
LOCUS       DAVLVZ010000057        10836 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000057 DAVLVZ010000000
VERSION     DAVLVZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10836
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.057"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            344..3955
                     /gene="rpoB"
                     /locus_tag="VYN77_001597"
     CDS             344..3955
                     /gene="rpoB"
                     /locus_tag="VYN77_001597"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1559036.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEELWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            3988..7665
                     /gene="rpoC"
                     /locus_tag="VYN77_001598"
     CDS             3988..7665
                     /gene="rpoC"
                     /locus_tag="VYN77_001598"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1559037.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGAIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            7776..8189
                     /gene="ndk"
                     /locus_tag="VYN77_001599"
     CDS             7776..8189
                     /gene="ndk"
                     /locus_tag="VYN77_001599"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1559038.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(9135..9767)
                     /locus_tag="VYN77_001600"
     CDS             complement(9135..9767)
                     /locus_tag="VYN77_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559039.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(9769..10536)
                     /locus_tag="VYN77_001601"
     CDS             complement(9769..10536)
                     /locus_tag="VYN77_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1559040.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
BASE COUNT         3208 a         2253 c         2342 g         3033 t
ORIGIN      
        1 attcgaatgt gatgccaagt tttctatggg aaaaaattta ccaaaaacct actgaaaccc
       61 cttgaaaaat ctagctaaat agggtataat atgggtaatc atttgtcgta ggttttgtct
      121 gaaatattgt ccagacaagg ctcacagcag ttaaatcttc tgaaaaagtc agatttagct
      181 gctctttttg tgcttttttt caagattatg agcatttgta acagaggctt aaagattctg
      241 aaaattcgtc aagaggacac ggtgataagg ggtttacaac catatgacga ttagaaaagc
      301 ctgattgaca aggcttggaa cttatttaca aaggagaatc atcttggcag gacatgacgt
      361 tcaatacggg aaacatcgta cccgtcgtag tttttcaaga atcaaagaag ttcttgactt
      421 accaaatttg attgaaattc aaactgactc attcaaagct ttcctagacc acggtcttaa
      481 ggaagtgttt gaagatgtat tgccaatttc aaacttcaca gacacaatgg agttggaatt
      541 tgttggatat gaaatcaagg aaccaaaata cacgctagaa gaagctcgta tccacgatgc
      601 tagctactca gcaccaattt ttgtaacctt ccgtttgatc aataaagaaa caggcgaaat
      661 caagacccaa gaagttttct ttggtgattt cccaatcatg acagaaatgg gtactttcat
      721 catcaatggt ggtgaacgta ttatcgtttc tcagttggtc cgctcaccag gtgtttactt
      781 taacgacaaa gtagacaaaa atggtaaggt gggctatggt tcaactgtta tccctaaccg
      841 tggagcttgg ttggaacttg aaagcgactc aaaagatatc acctacactc gtatcgaccg
      901 tactcgtaag attccattta caaccttggt tcgtgctctt ggtttctcag gtgatgatga
      961 aatctttgat atctttggtg acagcgaatt ggttcgcaac actgttgaaa aagatatcca
     1021 caagaatcca atggactctc gtacagacga agccttgaaa gaaatttacg aacgccttcg
     1081 tccaggtgag cctaagacag ctgaaagctc acgtagcttg cttgtggctc gcttctttga
     1141 cccacgtcgc tatgacttgg cagcagttgg tcgttacaaa atcaataaaa aactcaatgt
     1201 taaaacacgt ttgctcaacc aaaccattgc agagccattg gtagaccctg aaactggaga
     1261 aatcttggta gaagctggta cgattatgac tcgtagcgtg attgaaagca ttgaaagcca
     1321 tttggatggc gacttgaaca agattgtcta catcccaaac gatgcagccg ttgtgactga
     1381 gcctgttgtt cttcaaaaat tcaaggttgt tgctccaact gatccagatc gcgtcgtaac
     1441 gatcattggt aatgctaacc cagatgacaa ggttcgtacg gtgactcctg cagatatcct
     1501 tgctgagatg agctacttcc tcaacttggc tgaaggactt ggccgtgtag atgatatcga
     1561 ccaccttgga aatcgtcgta tccgtgcggt tggtgaattg cttgccaacc aagtacgttt
     1621 gggactttct cgtatggaac gtaatgtccg tgaacgtatg tctgttcagg acaatgaagt
     1681 cttgacacca caacaaatta tcaatatccg tcctgtaaca gctgcagtta aagaattctt
     1741 tggttcatca cagttgtcac agttcatgga ccaacacaac ccgctttctg agttgtctca
     1801 caaacgccgt ttgtcagcct taggacctgg tggtttgact cgtgaccgtg ccggatatga
     1861 agtgcgtgac gtgcactaca ctcactatgg tcgtatgtgt ccaatcgaaa cacctgaagg
     1921 acctaacatc ggtttgatca ataacttgtc atcttacgga cacttgaaca aatatggttt
     1981 tgttcaaaca ccataccgta aggttgaccg tgaaacaggt gttgtcacga acgaaattgt
     2041 ttggttgaca gctgatgaag aagatgaata tactgtagct caggctaact ctcgtctgaa
     2101 tgaagatgga acctttgctg agaagattgt catgggacgt caccaagggg tcaaccaaga
     2161 gtatccagct aatattgttg actacatgga cgtttcacca aaacaggtag ttgccgttgc
     2221 gacagcatgt attcctttct tggaaaacga tgactccaac cgtgccctca tgggagccaa
     2281 tatgcaacgt caggctgtgc cattgattaa tcctcaggca ccttacgttg gtactggtat
     2341 ggaataccaa gcagcccacg attctggtgc ggctgtgatt gctcagtatg atggtaaagt
     2401 tacttacgca gatgctgaca aggtagaagt tcgtcgtgaa gatggttcat tggatgttta
     2461 ccacatccaa aaattccgtc gttcaaactc aggtactgct tacaaccaac gcactctcgt
     2521 aaaagttggt gatgtcgttg aaaaaggcga tttcatcgct gacggacctt ctatggaaaa
     2581 tggagaaatg gcgcttggac aaaacccaat cgttgcctac atgacttggg aaggttacaa
     2641 cttcgaggat gccgttatca tgagcgaacg cttggtgaag gacgatgtct acacatctgt
     2701 tcaccttgaa gaatacgaat cagaaacgcg cgatacaaag cttgggcctg aagaaatcac
     2761 tcgcgaaatt ccaaacgttg gtgaagatgc cctcaaagac cttgacgaaa tggggattat
     2821 ccgtattggt gctgaggtta aagaaggtga tattcttgta ggtaaagtaa cacctaaggg
     2881 tgagaaagat ctttcagctg aagaacgtct cttgcacgct atctttggag acaagtctcg
     2941 tgaagtgcgt gatacttctc ttcgtgtacc acacggtgcc gatggtgtcg ttcgtgatgt
     3001 taagatcttt acacgtgtaa atggagatga gttgcaatca ggtgttaaca tgttggttcg
     3061 tgtttacatc gctcaaaaac gtaagattaa ggtcggagat aaaatggccg gacgtcacgg
     3121 aaacaaaggg gttgtctctc gtatcgttcc tgtagaagac atgccttacc ttccagacgg
     3181 aactccagtc gacatcatgt tgaacccact tggggtgcca tcacgtatga atatcggtca
     3241 ggttatggag cttcaccttg gtatggcagc tcgtactctt ggtattcaca ttgcgacacc
     3301 agtctttgat ggagcaagtt ctgaagaact ttggtcaact gttaaagaag caggtatgga
     3361 tagcgatgcc aagacaatcc tttacgatgg acgtacaggt gaaccatttg ataaccgtgt
     3421 ttctgttgga gtcatgtaca tgatcaaact ccaccacatg gttgacgata aattgcacgc
     3481 gcgttcagtc ggaccttact caactgttac ccaacaacca ctcggaggta aagctcagtt
     3541 tggtggacaa cgtttcggtg agatggaggt ttgggctctt gaagcctacg gtgcgtcaaa
     3601 tgtccttcaa gaaatcttga cttacaagtc ggacgatatc aacggacgtt tgaaagccta
     3661 tgaagctatt acaaaaggca aaccaattcc aaaaccaggt gttccagaat ccttccgagt
     3721 tcttgtcaaa gaattgcaat ctcttggtct tgacatgcgt gtcctagacg aagatgacca
     3781 agaagtggaa cttcgcgact tggatgaagg aatggacgaa gatgtcatcc acgtagatga
     3841 ccttgaaaaa gcccgcgaaa aagcagccca agaggctaaa gcagcctttg aagctgaaga
     3901 agctgagaaa gcaacaaaag cggaagcaac agaagaagct gctgaacaag aataagcagt
     3961 tcacttagaa tagaaaggga agaaatagtg gttgatgtaa atcgttttaa aagtatgcaa
     4021 atcaccctag cttctccaag taaagtccgt tcatggtctt atggagaagt caaaaaacct
     4081 gaaacaatca attatcgtac cttgaaacca gaacgtgaag gactctttga tgaagtgatc
     4141 tttggtccta caaaagactg ggaatgtgct tgtggtaagt acaaacgcat tcgttacaga
     4201 ggaattgttt gtgaccgctg tggggttgaa gtaacgcgta cgaaagttcg tcgtgagcgt
     4261 atgggacata tcgaattgaa agctcctgta tctcacatct ggtacttcaa ggggattcca
     4321 agccgtatgg gcttgaccct tgatatgagc cctcgtgccc tcgaggaagt tatctacttt
     4381 gcggcttatg tggtgattga tcctaaggat acaccacttg agcacaagtc tatcatgaca
     4441 gagcgcgaat accgagagcg cttgcgcgaa tatggttatg gttcatttgt tgctaagatg
     4501 ggtgcggaag ccatccaaga ccttttgaag caagtagatc ttgaaaaaga aattgctgaa
     4561 ctcaaagaag aattgaaaac tgctactgga caaaaacgtg tcaaagccat ccgtcgtttg
     4621 gatgttttgg atgcctttta caagtctgga aacaaacctg aatggatgat tcttaacatc
     4681 cttccggtta tcccaccaga tcttcgtcca atgttgcagt tggatggtgg ccgttttgcc
     4741 tcatctgact tgaatgacct ttaccgccgt gttatcaacc gtaacaaccg tttggctcgt
     4801 ttgcttgagt taaatgcacc aggtatcatc gttcaaaatg agaagcgtat gcttcaagaa
     4861 gcagttgacg ctttgattga caatggtcgt cgtggtcgtc caatcacagg accaggtagc
     4921 cgtccattga aatcattgag ccacatgctt aaaggtaaac aaggacgctt ccgtcaaaac
     4981 ttgctcggta aacgtgttga cttctcagga cgttccgtta tcgccgttgg tccaactctt
     5041 aagatgtacc aatgtggtgt gccacgtgaa atggcgattg aactctttaa accatttgtc
     5101 atgcgtgaaa tcgttgcccg tgatatcgtg caaaacgtca aagcagctaa acgcttggtg
     5161 gaacgcggag atgagcgtat ctgggatatc cttgaagaag tgattaaaga acacccagtg
     5221 cttttgaacc gcgcaccgac ccttcaccgt ttgggtatcc aagccttcga gccagtcttg
     5281 attgatggta aggctcttcg cttgcaccca cttgtctgtg aagcctacaa tgctgacttt
     5341 gacggggacc aaatggccat ccacgtacca ctttcagaag aagcacaagc agaagctcgt
     5401 atcctcatgc tagctgctga gcacatcttg aacccgaaag atgggaaacc ggtagttact
     5461 ccatctcagg acatggtttt gggtaactac tacttgacca tggaagaagc tggtcgcgaa
     5521 ggtgaaggaa tggtcttcaa agaccgtgac gaagcggtta tggcttaccg caatggttat
     5581 gttcacctcc actcacgtgt tggtatcgca acagacagcc tcaacaagcc ttggacagaa
     5641 gagcaaagac ataaggtctt gcttacaaca gttggtaaaa ttctcttcaa cgatatcatg
     5701 ccagaggggc taccatactt gcaagaacca aacaatgcca acttgacaga aggtgttcca
     5761 gctaaatact tcttgccact tggtggagct atcaaggaag ctatcagcaa tcttgagctc
     5821 aaccctccat tcaagaagaa aaacctcgga aatatcatcg ctgaaatctt caaacgtttc
     5881 cgtacgacag aaacttctgc cctacttgac cgcatgaaga acctcggtta ccaccactca
     5941 actcttgcag gattgacagt gggtattgcc gatatcccag tcgttgatga caaggctgaa
     6001 atcattgaag aatcacacaa acgtgtcgaa caaatcacca aacaattccg tcgtggtatg
     6061 atcacagacg acgagcgtta caatgctgtt acagctgaat ggcgtgctgc ccgtgaaaaa
     6121 cttgagaaac gcttgattgc taatcaagat cctaagaacc caatcgttat gatgatggac
     6181 tctggagccc gtggtaacat ctcaaacttc tcacagcttg ccggtatgcg tggtctgatg
     6241 gctgctccga atggacgtat catggaattg ccaatccttt caaacttccg cgaaggtttg
     6301 tcggtattgg aaatgttctt ctcaactcac ggtgctcgta aaggtatgac cgatacggcc
     6361 cttaagacag ccgactcagg ttacttgact cgtcgtttgg tcgacgttgc ccaagacgtt
     6421 atcatccgtg aggacgactg tggaactgac cgtggtctct tgatccgttc tatcgcagaa
     6481 ggaaaagaga tgatcgagtc tctcgaagag cgtctcaacg gtcgttacac taagaaaact
     6541 gttaaacatc cagaaactgg tgcagtgatt attggtccaa atgaattgat tacagaagac
     6601 aaggcgcgtg aaattgtcaa tgctggtgtg gaagaagtga ctatccgttc tgtatttaca
     6661 tgtaacactc gtcacggtgt ctgccgtcac tgttacggta tcaacttggc gactggtgat
     6721 gcggttgaag ttggtgaagc agttggtaca atcgctgccc aatctatcgg ggaacctggt
     6781 acacagctta caatgcgtac cttccacaca ggtggggttg cctcaaatac cgatatcact
     6841 cagggtcttc ctcgtgtcca agaaatcttt gaagcccgca atcctaaagg ggaagcggtt
     6901 attacagagg ttaaaggaca agttactgct atcgaagaag atgcatcaac tcgtaccaag
     6961 aaagtctttg ttaagggtga aactggcgaa ggtgaatatg tcgttccatt tacagctcgt
     7021 atgcgtgtcg aagttggggg ccaagtagcg cgtggtgctg ctctgacaga aggttctatc
     7081 caaccaaaac gtctccttgc agttcgtgat gtcttgtcag ttgaaacgta ccttctcggt
     7141 gaagtacaaa aagtttaccg tagccaaggg gtagaaatcg gtgacaaaca catcgaggta
     7201 atggttcgtc aaatgatccg taaagtccgt gtcatggatc caggtgatac agatcttctc
     7261 atgggtaccc tcatggatat caatgacttt acagatgcca acaaagatgt ccttatcgca
     7321 ggtggagttc cagcgacagg tcgcccagtc cttatgggaa ttaccaaagc ctcacttgaa
     7381 acaaacagtt tcttgtcagc ggcttccttc caggaaacaa ctcgtgtcct tactgacgca
     7441 gctatccgtg gtaagaaaga ccatctcctt ggacttaaag aaaatgttat catcggtaag
     7501 atcatcccag ctggtactgg tatggcccgt taccgtaacc ttgaaccaca tgctgtcaac
     7561 gaagaagaat accttaatcc tccagtagag gaagaaggaa atgaagaaac aacagaagta
     7621 gttgtggata ctgccgttga aactgtggaa gaaacagtag aataagagag aatgagagaa
     7681 cccgagggtt ctctcattct ctcttctgaa aactttctcc atttttccac gaaatgtggt
     7741 aaaataaaag aaagaagctg acaaaggaga cgggtatgga acaaacattc tttatcatca
     7801 aaccagatgg tgtaaaaaga gggctagtgg gtgaagtgtt aaagcgcatc gaacaacgtg
     7861 gatttacaat cgaaaaattg gagtttcgtt cacaggtttc agaagagttg attgaccagc
     7921 actatcagga cttggttggt cagagttttt acccaccgat tcgtgaattc atgacttcag
     7981 gtccagttct tgtgggtgtc atttctggtc ccaaagtaat cgaaacttgg cggaccatga
     8041 tgggtgcaac tcgtccagaa gaagctttac caggcactat tcgaggtgat tttgcaaaag
     8101 ctgctggaga aaatgagatt atccaaaatg ttgtacatgg ttcagattca gaagagtcag
     8161 ctaagcgaga aattgctctt tggttttaag agtggattgg ctcaatcaat tggttaaaag
     8221 ctcatttgaa tagaaagtat agtcaattag tttaagacat gacgcatgat atcaaacttt
     8281 ttagtttttg atatggtgcg ttttttgatt aagtgactat tagtttgtct cgctccgtta
     8341 ggtgcgagac aaaaaaaaca cccgctatgc gggtgtgcgt cgaaggttat accaaaaaaa
     8401 ctccaaacgc gatacaataa aggtgttcaa tccaattgta aagcgaaagg agaaaaatat
     8461 ggcacaaaag gttcttttat cctgttcttg tttcaattga ctatatttct atactttttc
     8521 ttcctttctc tgtgaaaatc gcaagttttt cccacctttt ctcaatactg aaacaacatc
     8581 tttccagaac ttcaataatt tctttttgtg ttaaatcatt atttaactaa atctttggag
     8641 gcaatacgtt ttcaaagcta cttttttacc ataggttttg agccttcaac acagattact
     8701 aagccaaaaa tgctctaaat ttttgaaatt ctaacaaatt ccactcaact ttatcatcat
     8761 ctttgacttt cacaatatct tcctcaaaat aacatggaat aaagatagtt ttcttttaaa
     8821 acggttgtag gaggaaaatc tctcctacac tattattgat tctgagagaa aattgcaaaa
     8881 tatcatctac caaactttca attattaaga ttttttgcag tttatctgaa ttagaatcgc
     8941 aaggcttctc aataatataa ttgtcgaatt ttttcattct aacttcctct ccattatttc
     9001 ttcctttatc aagaaaaagt tacctcaata ttaataaagt acaattctaa attaagtaca
     9061 attctaaatt aactccaatt cttgattcat gataatattt cagtaactca tcgtgcaatt
     9121 aaaaacaagg atttttattt cagatgatcc atcgtgaaca cttcatcagc catctcccaa
     9181 attgccggat tatgtgttgc gataatgatt agcctattat catctcgaag agatagcaaa
     9241 atctccataa tcaactgaga ggttgctggg tctattgaag ctgttggctc atctgccaga
     9301 ataaagggtg gattctttaa gataattttt gccaaggcaa cccgttgcga ttctccgccc
     9361 gataactcaa agatgcgctt atctaggtca agataaacca ggccgacctg ttctaaagcc
     9421 tgcttctgcc tcaaccgctg ttccgaccga ctcaactttt gaccaatgag acctagctta
     9481 aggttttctt caatactttg gttttcaatt aagccaaagt tctggaagag gtagcccaat
     9541 tcgtgacgga aaaaatcact tgatttataa ttggccaagt ctttacctcg gtaaaaaatc
     9601 gtcccatcat aaggttctaa tttcccaatc atgttcatca aggttgtttt tccgctacca
     9661 cttgaaccaa ttaaggcata gacttttcca gcctcaaatg tcattgaaag attcgaaaat
     9721 aactctcgtt ctccaaaaga tttactcacc tgtttaagtt caatcatatt aacctccctt
     9781 caaaacaagc atggacatct tgttttcttt ctgcatttgg acatgtaact gtaaaagaga
     9841 tagaccagta aagagtaaca agactaagaa agcaactcct atctctacct gaagaaatac
     9901 actcgcaaca aatcccagta aaaacacacc cagttgagca aagagataag tgcgatggat
     9961 ttctaagaac ctgagacctg caatgcgttt gataaaaatg gcacgtctaa attcttcaaa
    10021 gtagagccta ttcatagtgt taaacaacaa gattgaagtt gcaatcccaa gcacagctcc
    10081 tgctagcatt acccaacgtt ccctctggat attatccaat aatgtgatgt agttgtggta
    10141 acctgtttgc atttctgaga cccaattttc aatgccttgt ctctggataa gctcctgggc
    10201 atcagacaat tgattaaaga gaacgtagtt ctgtactgcg tctatccaaa acaaaatgga
    10261 ctgtggacca gttgattggg gtgttataac aatgatgatt ggatctttca aaaactgctg
    10321 gtaagaaata ggggtcgtat tatacacaaa acgatcctga cctgattcta aataacctac
    10381 cgtagcagtc atttgctgtc gttcatcttg actagatatg cgactggtta agtcgtcttc
    10441 aaaaacagat ttataatgtt cttcctctga acggaggtgt tcaggcagca ataagacaaa
    10501 ctcccctaca tcaaggtgat tcatcttttg actcatttta gttgaactgt aggacttact
    10561 ttttcagagg gctccaaatt ttattttcat ttactttttt tagcccataa aatatttacc
    10621 atacttataa aaagtagtaa tactccaact aaactgactt gtaatgaatt atatgtattt
    10681 tctgaagtgt taggtaaata attttgagag tacccttctc gggcaatagt aataatattg
    10741 tgtgtgttta tttgtttaat tccaatagta gtttgaattc tctcttccaa ctcaattaaa
    10801 ttaacattat ctatgaaact atcccaactt tgttca
//
LOCUS       DAVLVZ010000058        10716 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000058 DAVLVZ010000000
VERSION     DAVLVZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.058"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(78..1502)
                     /locus_tag="VYN77_001602"
     CDS             complement(78..1502)
                     /locus_tag="VYN77_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1559041.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVTWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2047..4326)
                     /gene="pulA"
                     /locus_tag="VYN77_001603"
     CDS             complement(2047..4326)
                     /gene="pulA"
                     /locus_tag="VYN77_001603"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1559042.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4438..6396)
                     /gene="ligA"
                     /locus_tag="VYN77_001604"
     CDS             complement(4438..6396)
                     /gene="ligA"
                     /locus_tag="VYN77_001604"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1559043.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6489..7667)
                     /locus_tag="VYN77_001605"
     CDS             complement(6489..7667)
                     /locus_tag="VYN77_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1559044.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFFRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7747..8610)
                     /locus_tag="VYN77_001606"
     CDS             complement(7747..8610)
                     /locus_tag="VYN77_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1559045.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8613..10514)
                     /locus_tag="VYN77_001607"
     CDS             complement(8613..10514)
                     /locus_tag="VYN77_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1559046.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIASLEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQNQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3212 a         2360 c         1910 g         3234 t
ORIGIN      
        1 ttcgtatttt ttctataatt attattattt tatagcaaaa aaataaaacc aggaaaacaa
       61 tttcctgatt ttacacgtta tttcacatca aaaacaatgg atttgacatt tgtcatcgct
      121 tcaatgctat atttaattcc ttgcactcca gctccagaac ctttgacacc aaggaatggg
      181 aaattatctg gaccacgctg ggttttatta ttaatgtgga ctgtacctac ttcaagtttt
      241 tcagcaattt caaaggcttt tttgaaatca tttgtaaaga ctgatgattg aaggccgaat
      301 tcagattcgt tggcaaaggc aatagcttcc tctacactag ccacacgaat gattggtaaa
      361 acaggaccaa atggctcttc ccatgtcact ttcatatctt ttgtaacttg gtcaaaaagc
      421 actggccaga ggagattgcc ctcacgtttg attggtgtaa gagcctgagc ctctttttct
      481 tgtgcatcct caatcaagcc ccaaatgaag tcggctgaag cattgtcaat aacaggtgta
      541 atatcagcat tgtcaaatgg atcaccaact gttaatttag aaacttcttc ctgaagcaaa
      601 gtagctaatt tatctgctac actttcgaga acaatgacac gtttaatggc cgtgcaacgt
      661 tgtcctgagt agctaaaggc tcccgcaaca atttgcttgg cagcatgttc caaatctgca
      721 tcttctagta caagagctgc atctttccca ccaagttcca acatgatagg acgcatacca
      781 gctaaacgac caatacgttc tccaatagga gttgaacctg taaagttgat gaagttgact
      841 tctttgtgct caatgatata atccccaatt tctgaaccac gacctgtaat ggtgttgaaa
      901 acacctgccg gaatccctgc ttcttcaaat gctttagcca acaagagtcc agaaatggaa
      961 ccttgtgttg gtggcttcaa catgaccaca ttccctgcaa tcaaggcagg tgcaatttta
     1021 gaagcagata aattaactgg ataattaaag ggagcaatcg ctagcacgat accaactggt
     1081 tcacgacgga caacagccag tttgttttta cttgctgcct caaaaccacc accttccatt
     1141 gcttgtccag tgatacggag accttcctca gcagcataac gaatcaagtc tgctgtacgc
     1201 actacttctc caattgctgc tttaatccct tttgctactt ctttggcaag gatagtacca
     1261 attttttcct tatcgcgttc taaaatagct gctgttttat gcaaataagc cgcacgttca
     1321 attgctgata aagctcgcca tgctggcagg gctgcacgcg cagcttgcat agcctcatca
     1381 gcttcagtct gagtcatggc tggaactgta cccaattctt cttgattgat tggtgaataa
     1441 atcgtaattt cttgttcaga tgatttccat tttccattta ctaaattctg atatcttgtc
     1501 aaattcttgt cctcctgaaa atgattaaga tatattttac caaattttca gaaaatcaca
     1561 accctttttg caaaattttt gagattattt tcacaatctt gatttttcaa agtatactca
     1621 ataaaaatta aaaaaatcca ctacgtcaag gcgaggctaa tgtggtttga agaaattttc
     1681 gaagagcgtg aatgagtatc atctatagta aaataaaaaa actgaacaat ttggttgggg
     1741 acagccaaac caatttctca caatgtttca gaaacaaggg tgtgctattc caatttcagc
     1801 ctactataac tgtcatagat tgctgaaaca aagtctaggt aaaagtcttc ataataaaaa
     1861 gacctcctat caagtgttca aaaactttga taggaggtct tgttttgtga aaatatttat
     1921 caaattttct atacaagtga gctgttagcc aggttctttc tattctttca atttcaatga
     1981 atggattttt tactaatact cataactggg aatttgtctg tgtaaaaata gcgagataga
     2041 tggtatttat aaaacactca agacagctag actaatatca tttaaaacat tatcttcttt
     2101 tgagcgactg ttggttacca acatagctaa atttcctgca ttttcaaatt gatagggttc
     2161 tgatttagca ttcacaacta ccaagaggtg ttctttgccg tgaacttcat agataaggca
     2221 gccgctatgt tcaatcgcag aatgcacaaa gacatgatgg taaatttcat catagctaga
     2281 gtaagaaaag gcaccagttt ttgtcttcaa tcggatgact tgacggataa actcaatact
     2341 gtcttgacgc tcattaatca agttccagtt cacttggttc acactgtcag gagcattata
     2401 gctattcatc gcacgctctc tatcatcatg ggtcaactca ccattttcac cagtcgcaac
     2461 cagtttggta cgaccaaatt cttgaccgat ttccataaag gccatcccct gcatgagcag
     2521 attcatggct gtggcagttt cgaccttgcg catgatttgc tctgaacttt ggtctggatg
     2581 aagggttgcc aataaatcgt gaagattgta attgtcatgg gcttctacat agttaagcac
     2641 ctgatttgga tgtgtatagc ttcctaattc acgacttcct aggattgctt tagctagaat
     2701 tggctctgtc gcagcaccac tgacaaaacc tgacttgata gcaccataaa cttctccccc
     2761 tttgacagca tcgcgctgat tgtcattaaa gaaaccaata tttggcatct ggtaggcatt
     2821 gtccttcttg gccttatcat aaggggcaag acctgttccc atatcccatc cttctccata
     2881 gaggataatg ttggagtcga tttcatccaa gctttgacga atcatctgca tggtcttgac
     2941 atcatgaatc cccatcaagt caaaacggaa gccgtcaata ttatattcct gcacccagta
     3001 tagaagagaa tcaatcatat acttgcgaaa catttcgtgt tcactggctg tttcatttcc
     3061 aacacccgtt ccattctgga aggtaccatc tggattcata cgataatagt aatcagggac
     3121 tgttgtttgg aatggtgcat caacaactga gaaggtatgg ttatagacta catccataat
     3181 gactccaata cccgcatcgt gataagcttg aaccatcacc ttcaaatcac gaatgacctg
     3241 agctggatca tctggattag ttgaaaaact agtttctggc gcgttatagt tttgtggatc
     3301 ataaccccag ttgtaggtta catttccatc ctcatcgtat tctttatgac ggtctgcaat
     3361 tggttgcaat tgaacataat tgtagcccag cttcttgatg taatcaaaag cagttgactg
     3421 gccgtattgg ttaactgttc cagcctgagc agcacccaag aaagttcctc gaagatgttc
     3481 atctacaccc gatgtaggtg atttagtcaa atcacgaatg tgcatttcac agataactgc
     3541 cttacatgga ttttccaagc gccaagtagc ctccgaaccg tgcttaacct cgaagttttc
     3601 aacttgcttt tctacatggc tcagaatagc tgaacgtttg ccatcagggc tggtcgcgat
     3661 tgtataagga tcacgtgtca gtgtttggtg atgagggaat tggacttgat actgataagt
     3721 cttacctacc aaatcttctt caacatccaa actccagaca ccgattgtat tgtccttatg
     3781 attataagag tagctattgc ctcttttcat ctcaaaagtc ttccaaacgg gtgcatcatt
     3841 agcagctgat tcataaacga caacttgcac ttctgtcgct gtaggtgacc agagagaaaa
     3901 atgagcctga ttgtcctcta cacggcaacc caattctcct tggtaacccc aatgatgatc
     3961 aaaactagca ctgttaatgg ccttatcaaa ggcaaaagga ttttgatttt tatagaaagg
     4021 actggcaata gcaggatttt cagagtaata aatcctatca tcgccttcca aaatccagac
     4081 ctctgttaat aggggatagt gattaaaacg gatagaatat tctttactag tttgacctgt
     4141 atgaaccaca aaattcaagc tttctataac atgtgaactt gggtgttcaa agctaaataa
     4201 agctccaaaa taatcttctt tgtaggttag caaatcaatt cgttgatcct gactttttac
     4261 aaaggagcaa gtgtcatatt ctccattctt acgatggtaa tgaatgcgca tagggtagtt
     4321 atacattttt tatttttcct ttttactttg tttctatttc actaataaat ttttgtcaat
     4381 ctcgtctcaa ttaacagaca tagtcatatt ctctaaactc tgtttttaaa cgatccatta
     4441 caaactttct agccatgcct catctctgac ctggatacca agttcttgtg ctttttgcag
     4501 tttacttcca gcgtctgcac ctaccacgac gaggtcggtc tttttagaaa tactacctgt
     4561 cactttggca cccagacttt cgagtttact tttagcttct gagcgcttga gtcgttccaa
     4621 ttttcctgtc aataccacgg tcaaacctga caaggccgca tccgctacta ccgtctgtcc
     4681 tttatagtcc agattgaccc cagtttcttt caattctctg agcagaattt cagagccttc
     4741 tgtcgcaaaa taagtctgaa gacttttggc aatcacgcca cctagacttt caatactagc
     4801 cacttcctct gaatctgcct gatacagatt ttcaattgaa tggaaatatt gaagtaaaag
     4861 ctgactagcc ttgcttccga catgacgaat tcccaaacca aataagagct tctcggcaga
     4921 attttccttt gatgcttgga tagcctgata cagtttagca gcggactttt ccttaacccc
     4981 ctctaaaagg aggaaatcct cttcttgcaa acgataaata tccgccacat ccttgactaa
     5041 attagcagca aaaagcttct caacaataga tggaccaagg cctgtaatat tcatagcatc
     5101 acgagaagca aagtgaatca agccttccat gatttgagca gggcaacgcg gattgataca
     5161 acgtagggcc acttcatctt caaagtgcaa caagtcagag ttacaacttg gacagtttgt
     5221 agggatatct agtttttctt cagaaacccg tttggactct accacacgta aaacggcagg
     5281 gatgatgtca ccagccttat atacaatgac cgtatcgtct tttcggatat ctttttcagc
     5341 aatataatct acattgtgca gggtcgcacg gctaacagtc gtaccggcaa gttgtactgg
     5401 tgttagatta gcagttggag ttacaacacc ggtacggcca actgtccagt caactgataa
     5461 gagttgagct tctttttctt cagcagggaa cttgtaggct actgcccact ttggagcctt
     5521 aacggtaaaa ccaagttctt cttgacttgc taggtcgttg accttgatta ccactccatc
     5581 aatatcgtaa ggtagatttt cccgttcctg tcctacttct tggataaaat tccagatttc
     5641 atctatgttt tcagccaaga ttcgcttagg attgaccaca aaacctagtt gttctaggta
     5701 cttcaaaccc ttttcttggc tatcacgagt tgaagggctg gcttcttgat agagaaacgt
     5761 tgcaagatta cgcttggcaa ctactgctgt atccaactga cgcagagttc ctgctgccgc
     5821 attacgagga ttagcaaatt caggctctcc attttcttgg cgcgcttggt taacttggtc
     5881 aaaggaagcg cgtggcatgt aacattcccc acgaactgtg atatctagtt cttctggcaa
     5941 agtcaaaggg atgtccttaa cacgcttgag gttttctgtg atattttcac caattgaacc
     6001 atctccacgt gttaccccag caaccaaaat ccccttttca taagtcagcg agatagataa
     6061 gccatcgatt ttcagctcac aaatataggt cggatgagcc acttccttac gaacacgcgc
     6121 atcaaaagca tctagctcct cacgtgaaaa agcatcctgc aaactataaa gaggatactg
     6181 atgactgtat ttttcaaaac catctaaaac cttgccacca acacgatgag tcggactgtc
     6241 tgctagcact tgctctggat aagcagtttc taactcgacc aactcacggt aaaggcggtc
     6301 atactcactg tctgaaaccg agggattatc gctggtatag tactcagtcg catagcgatt
     6361 gagcaaagcg actaactcat tcattctttt attcataaga ccattttacc ataaaacaag
     6421 ccctcctcac aaacgagaag ggcggaaaaa acacttagtt tgaaattatt tttgaaactc
     6481 aagcaacctt atatcaattt ttcaaaatga gttcgaacat aaataaacga tatacaagac
     6541 aagatgataa caccacttcc aattatcagg aaagaagaga gatgtacact tggcaagact
     6601 gtcataaatc cttttgcaat aggcataaat agaatagcta aggtaaaaat tgtactcagt
     6661 actcttccaa gaaattcgct ctcaaccttg gtttgtactt gagtaaaaaa gtgaatatta
     6721 aaaatcgtca taaacaattc acaaactaaa tttccagaaa aggaaagaaa agttggaagt
     6781 ggtaatccca tcataaaaac tccgacacct gtcaaagcca gtaaaatcaa aagattataa
     6841 atattagctt taattttact agctagaaga gccccaatga tggaaccaat agcccccata
     6901 gttaaaatac ttgcataggc tccttctgac ccgtaaagct gattcgaaaa gggaagtaga
     6961 aattcaaaag ctgcaaaaaa gaaattaacg ctggaagcta ccagcaaaag gaagaaaatt
     7021 tcttgctgat gccagatata gtgtaaccca tccttgatat ctacaaaaat atctctccca
     7081 gtaaaagcct ttttctcttg aacttttgct tcctcttttg gaaggaaagc cactagaaca
     7141 aaagcaatga aaaaagtcag cgagtctagc agtagcgtca tatggagact tgcaaactgt
     7201 aaaacaagga aggaaagaac aggagagcta acacctacaa cctgcaaaac cagctctaag
     7261 cgagaattat agatcacaat ctcatctttc tccaccactt cagttatgat agctttattg
     7321 gctgtgcgag aaaaggcaaa agcaatagcc tgcacaatgt tagcaacaat caaagcgcca
     7381 atcatccagc tatcattcct tatgaaagaa atagccagac aaagaatccc acaaacaaga
     7441 tctgccgtca ttaaaatctt acgacgaaaa aaacggtctg aaataactcc gccaaaggga
     7501 ttgacgagaa tagatgtgac gagctcagaa atctgataca ttcctaaaac tgtctgtcct
     7561 atagtcccca tagaagccaa ccagacacta tttccataat catagagcat atttcccatt
     7621 ttattgatag ccccacggct aatcaactgc actgcatagc gattcatatt aaagctcctc
     7681 tcaaattttg aaactattgt atcaaaaccg aaaggagctt tttatttttt cccttatttg
     7741 ggaaaattaa cttttgacaa atttttcgta gtgttcctga taataggcta cttgctctgg
     7801 aagacctaac acatcaaaaa tatgcatggc ctcttgcatc tgcttacagc cttctttaca
     7861 ctgtcctttt tgatataagg caaaaccttt taaataatgg aaaacattac gctcataaag
     7921 cttaatacct ttgtcaataa tcttctctgt ataagcctca aaatagttgg cattataaaa
     7981 agaagaatgc tctaaacaat gctggtaaca attgagggcc aaaatcaaca ctaatctctt
     8041 atggcgacta atctcttggt aaaattcctc cctctccata acttctctac caatccgagt
     8101 gacatagtct acatcgtaga aactatagag gttaccgaaa agaatcaact catacatggt
     8161 ccattcttct gttttgaaga gataatctgc taccttatcc aaatcatcct gcttcatatc
     8221 ataactcgca tctctttgac aaatcagacc ttgtagcaaa atccagttca gctcaaaata
     8281 aaggggagtc gtcgaactct tagacttttc aagttgttct ctttgaagct tttgaaaacc
     8341 tgcaatatcg tttgaatagt aaagtgggat aatctgtgcc atcatagaca catgttcatg
     8401 attatgaaaa ttccttgcct tatccatgaa attttcgatt gttacatgaa tgttatccaa
     8461 aatctcaaag aaacgggaga ctgccaggtc agactcccca agctcaaagc gagataactg
     8521 agaggtagag caggattcgc ctgctgcttc ctttaaagaa taatttccac ttgttcgaaa
     8581 ttcacgaaat acttttccaa gatgttccat ctttacacct gctctgataa ttcttcccac
     8641 tcaagcatag cttcttcctg acgatggctg attttgtcca gctcagcctg taattccatg
     8701 agtttgtcgg catcgtttgt ttccaacatt tgttcagaaa tggcttggtt ttgactttct
     8761 agctcttcaa tttcagcttc tagactttcg atttgtcgca tgagtttgcg aacttctttt
     8821 tgactttctt tctgggcctg atagtcattg actggacttg cttcctttgc ttgattgcta
     8881 gttgaagctt cctcagtctg actcatttct gctgttgctt tcttctcaac atagtagtcg
     8941 taatctccaa ggtagagagt tgaaccattc tcagacaatt ccaaaacatg agttgccaca
     9001 cgattgataa agtaacgatc atgactgaca aaaagcaagg taccatcaaa gtcaatcaag
     9061 gcattttcta gcacttcctt actatcaata tccaagtggt tggtcggctc atccagaatc
     9121 aaaaagttat tgttttccat agacaattta gctaaaagca aacgagcttt ttcgccacca
     9181 gatagcatgc cgactgattt tttaacatca tctcctgaga aaaggaaggc tccaagacgg
     9241 ttgcggattt caacttctgg tgtcagtttg aaatcattcc agagttcatc cagcaccgta
     9301 ttacttggtg tcagcttgct ttgggtttgg tcatagtaac caacctcaac attagcgcca
     9361 aagcgctttt cacctttgat aaaaggaatc tggtccacaa tagacttgat aaaggttgac
     9421 ttgccgatac cattgggacc aacgatagca acagcattca tcttacgaag atctaggtta
     9481 atcggttgtg acaagacttc cccgtcataa ccaatagcag tattttcaac agtcaaaaca
     9541 acattgcccg acgttttttc agactggaag gtcatgttgg ctgctttctt gccagcttca
     9601 ggcttgtcca aacgctccat tttttctagt tgtttacggc gagattgagc acgtttggtc
     9661 gttgaagcac gaactagatt gcgattgaca aagtcttcca gagaagcgat ttccttctgt
     9721 tgcttttcat agttttttgc ctcagtagct agcttttgct ccttcaactc gacaaaacga
     9781 gagtaattcc ccacatagcg atccaaggaa tgcttggtca aatctagcgt aattgtcgca
     9841 accttgtcca agaaataacg gtcgtggctg acgataatga gggcaccgct atagtttacc
     9901 aagtaattct ctagccaggc gatagtttca atatccaagt ggttagttgg ctcgtccaag
     9961 accaagagat tgggcttttc aaggagcatt ttggcaagtg ccaaacgagt attttgacca
    10021 ccagaaagct cagcaatttt catctgccac atagactcgt caaacttgaa tccattcaaa
    10081 atcgctcgaa tatcagcttc ataggtaaag ccacctgctt ggcgaaaatt ctcagataag
    10141 cggtcataat ctgacatcag tttatccaaa tcctcaccag acttttcacc catctccagc
    10201 tccatctgac gcagttgtct ctccgtccga cgcaaatcat taaagacatg aagcatttca
    10261 tcgtagatgg tattttcaga ctcaaaacgg ctatcttggg ctaggtaaga cagagaaata
    10321 tcttttttct tattgatttc tccgctagtt ggctcctctt ctccaactaa aatcttcaaa
    10381 agagtagact tacctgcacc atttttccca acaagggcaa tccgatctcg ttcatcaacc
    10441 tgcaggttga tattatcgaa aagaacctct cctgcaaaag aacgttcaat tttattagct
    10501 tgtaaaataa tcataagagt agtatagcat gtttccctaa ggcattcaag ataatcgtaa
    10561 gtcttttagt acaactttta taacataaaa taaactaaat tatgtatatt ttatattaga
    10621 ttcttcacta tcttgttgga ttttctaacc agctaatctt gtttcaaata gttatcgcac
    10681 aagcctatta tttaattctt ttcattattt acgtac
//
LOCUS       DAVLVZ010000059        10679 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000059 DAVLVZ010000000
VERSION     DAVLVZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.059"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            953..2212
                     /locus_tag="VYN77_001608"
     CDS             953..2212
                     /locus_tag="VYN77_001608"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1559047.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2228..2779
                     /locus_tag="VYN77_001609"
     CDS             2228..2779
                     /locus_tag="VYN77_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1559048.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFSE"
     gene            2772..4049
                     /locus_tag="VYN77_001610"
     CDS             2772..4049
                     /locus_tag="VYN77_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1559049.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            4065..4925
                     /locus_tag="VYN77_001611"
     CDS             4065..4925
                     /locus_tag="VYN77_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1559050.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDITKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            5387..6110
                     /locus_tag="VYN77_001612"
                     /pseudo
     CDS             5387..6110
                     /locus_tag="VYN77_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            6301..8592
                     /gene="pcrA"
                     /locus_tag="VYN77_001613"
     CDS             6301..8592
                     /gene="pcrA"
                     /locus_tag="VYN77_001613"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1559051.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            8635..9315
                     /gene="radC"
                     /locus_tag="VYN77_001614"
     CDS             8635..9315
                     /gene="radC"
                     /locus_tag="VYN77_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1559052.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9312..10001)
                     /locus_tag="VYN77_001615"
     CDS             complement(9312..10001)
                     /locus_tag="VYN77_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1559053.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(10019..10660)
                     /locus_tag="VYN77_001616"
     CDS             complement(10019..10660)
                     /locus_tag="VYN77_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1559054.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
BASE COUNT         3203 a         1923 c         2376 g         3177 t
ORIGIN      
        1 actatattgt tataaattga tttgaatttc aaaattaaat tgtttgattc ttatttcaat
       61 ttgttatagt atatctgatg tcaaagttct cggcgagtca aatagcgatt cccaagcctg
      121 actatcgtga ggtagcggat taaaatggtc tggggataga ccgttttaag tctgacgctg
      181 gaaataagaa ttgtcagaag aagggatagc gaactcgtgg ctctacgaac aggaacgtga
      241 taataaggcg tatatagcgg ataagagggc ctcaaactct aaagtccaaa aaggtagtcg
      301 taacctatat gcgtaaatta cgagagtaat tgaattcgta ctaagatttt ctattttcac
      361 tgtaagcttt taacgccctt atatcttgta tacacgagga aagatgtacg acttatcccg
      421 tgaggtctat cactataaag agaaaacgac agatagaagt gatcctgagt cacggttatc
      481 tttaaaatag attgtctgat aggacggtat gtataaaacg cttctgtgaa ctgagagaag
      541 ggggagaagt tcttgctaaa atttagttga acagccgtat tccgatactt agataagaga
      601 tctagtctta gctcctactc agttttaggg gataaaaaag gggcaatagc gattcgagaa
      661 agattatact cttcgaaaat ctcttcaaat cacgtcaata tcgccttgtc gtatgtgtag
      721 gatactgact acgtcagttc catctacaac ctcaaaacag tgttttgagc aacctgcggc
      781 tagtttccta gtttgatctt tgattttcat tgagtattag taattcagtt actaactcgt
      841 caactctgat ttatccaata aaattgaaaa ggatggaaaa aaggataaat ttatgatata
      901 ctttattttg aagaccttat tagaaatctt gaaagagtat tgaaaactta gaatgagaaa
      961 aattgttatc aatggtggat taccactgca aggtgaaatc actattagtg gtgctaaaaa
     1021 tagtgtcgtt gccttaattc cagctattat cttggctgat gatgtggtga ctttggattg
     1081 cgttccagat atttcggatg tagccagtct tgtcgaaatc atggaattga tgggagctac
     1141 tgttaagcgt tatgacgatg tattggagat tgacccaaga ggtgttcaaa atattccaat
     1201 gccttatggt aaaattaaca gtcttcgtgc atcttactat ttttatggga gcctcttagg
     1261 ccgttttggt gaagcgacag ttggtctacc gggaggatgt gatcttggtc ctcgtccgat
     1321 tgacttacac cttaaggcgt ttgaagctat gggtgccact gctagctacg agggagataa
     1381 catgaagtta tctgctaaag atacaggact tcatggtgca agtatttaca tggatacggt
     1441 tagtgtggga gcaacgatta atacgatgat tgctgcggtt aaagcaaatg gtcgtactat
     1501 tattgaaaat gcagcccgtg aacctgagat tattgatgta gctactctct tgaataatat
     1561 gggtgcccat atccgtgggg caggaactaa tatcatcatt attgatggtg ttgaaagatt
     1621 acatgggaca cgtcatcagg tgattccaga ccgcattgaa gctggaacat atatatcttt
     1681 agctgctgca gttggtaaag gaattcgtat aaataatgtt ctttacgaac acctggaagg
     1741 gtttattgct aagttggaag aaatgggagt gagaatgact gtatctgaag acagcatttt
     1801 tgtcgaggaa cagtctaatt tgaaagcaat caatattaag acagctcctt acccaggctt
     1861 tgcaactgat ttgcaacaac cgcttacccc tcttttacta agagcgaatg gtcgtggtac
     1921 aattgtcgat acgatttacg aaaaacgtgt aaatcatgtt tttgaactag caaagatgga
     1981 tgcggatatt tcgacaacaa atggtcatat tttgtacacg ggtggacgtg atttacgtgg
     2041 ggccagtgtt aaagcgaccg acttaagagc tggggctgca ctagtcattg ctgggcttat
     2101 ggctgaaggt aaaactgaaa ttaccaatat cgagtttatc ttacgtggtt attctgatat
     2161 tatcgaaaaa ttacgtaatt taggagcgga tattagactt gttgaggatt aaaccgtaga
     2221 ggtgtttatg aatatttgga ccaaattagc aatgttttct ttttttgaaa cggatcgctt
     2281 gtatttgcgt cctttctttt ttagtgatag tcaggacttc cgcgagatag cttcaaatcc
     2341 agaaaatctt caatttattt tcccaacgca ggcaagtctg gaagaaagtc aatatgcact
     2401 ggccaattac tttatgaagt cccctttggg agtgtgggca atttgtgacc agaaaaatca
     2461 acaaatgatt ggttctatta aatttgagaa gttagatgaa atcaaaaaag aagctgagct
     2521 tggctatttt ttgagaaaag atgcttggtc gcaaggattt atgacagagg ttgttagaaa
     2581 aatttgtcag ctttcttttg aggaatttgg cttaaaacaa ttatctatca ttacccacct
     2641 tgaaaatgaa gctagccaaa gagttgctct taagtctgga tttagtttgt tccgtcagtt
     2701 taagggaagt gatcgttaca caagaaaaat gcgggattat cttgaatttc ggtatgtaaa
     2761 aggagagttc agtgagtaag catcaggaaa ttctaagcta tttggaggaa ttaccagtag
     2821 gtaaaagggt cagtgttcgt agcatttcga atcatctagg agttagtgat ggaacagcct
     2881 atcgggctat taaagaagct gaaaaccgtg gaattgtgga gacccgtcct agaagtggaa
     2941 caattcgtgt taaatcccag aaagttgcta tagatagatt aacgtttgct gaaattgcag
     3001 aagtgacttc ttctgaggtt ctggctgggc aagaaggttt agagagagaa tttagtaagt
     3061 tttcaattgg tgccatgact gaacaaaata tcttgtctta ccttcatgat ggggggctct
     3121 tgattgtcgg agaccgaacc cgtattcagt tgctagcctt ggaaaatgaa aatgcagttc
     3181 tggttacagg gggatttcag gttcatgatg atgtgcttaa actggccaat caaaaaggga
     3241 ttcctgttct aagaagtaag catgatacct ttaccgtcgc gaccatgatc aataaagcct
     3301 tgtcaaatgt ccaaatcaag actgatattc tgacagttga gaaactttat cgccctagtc
     3361 atgagtatgg ttttctgaga gagacagata cagttaaaga ttatttggac ttggttcgta
     3421 agaatcgtag cagccgtttc cctgttatca atcaacatca ggtcgttgtt ggcgttgtaa
     3481 ccatgagaga cgctggtgat aaatcaccaa gcacgacaat tgataaggtt atgtctcgta
     3541 gtctattttt ggttggatta tcgacaaata ttgccaatgt gagtcaacgg atgatcgcag
     3601 aagactttga aatggtacca gttgttcgaa gcaatcaaac tttgcttggc gttgtgacgc
     3661 gacgagatgt catggagaag atgagccgtt cccaagtttc ggctctacca actttttctg
     3721 agcagattgg acaaaagctc tcttatcacc atgatgaagt agttattaca gtggaaccct
     3781 ttatgctaga aaaaaatgga gttttggcta atggtgtatt ggcagaaatt ctgacccaca
     3841 tgacccaaga tttagttgtt aatagtggtc gcaatctcat tatcgagcag atgctgatct
     3901 actttttgca ggctgttcag atagatgata tattgcgcat tcaggcacgg attattcatc
     3961 atacgagacg gtcagctata attgattacg atatttatca tggtcaccag attgtttcaa
     4021 aagcaaatgt gactgttaaa attaattaga aactaggaga aaagatgata acattaaaat
     4081 cagctcgtga aatcgaagct atggacaagg ctggtgattt tctagcaagt attcatatag
     4141 gcttacgtga tttgattaaa ccaggcgtag atatgtggga agttgaagaa tatgtccgcc
     4201 gtcgttgtaa agaagaaaat ttccttccac ttcagattgg ggttgacggt gccatgatgg
     4261 actatcctta tgctacctgt tgctctctta acgatgaagt ggctcacgct ttccctcgtc
     4321 attatatctt gaaagatggt gatttgctca aagttgatat ggttttggga ggtcccattg
     4381 ctaaatccga cctgaatgtc tcaaaattaa acttcaacaa tgttgaacaa atgaaaaaat
     4441 acactcagag ctattctggt ggtttagcag actcatgttg ggcttatgct gttggtacac
     4501 cgtccgaaga agtcaaaaac ttgatggata taaccaaaga agctatgtac aagggtattg
     4561 agcaagctgt tgttggaaat cgtatcggtg atatcggtgc ggctattcaa gaatacgctg
     4621 aaagtcgtgg ttacggtgta gtgcgtgatt tggttggtca tggtgttggc ccaactatgc
     4681 acgaagaacc aatggttcct aactatggta ttgcaggtcg tggactccgt cttcgtgaag
     4741 gaatggtctt aaccattgaa ccaatgatca atacaggcga ttgggaaatt gatacagata
     4801 tgaaaactgg ttgggcgcat aagaccattg acggtggatt gtcatgtcag tatgaacacc
     4861 aatttgtcat tacgaaagat ggacctgtta tcttgactag ccaaggtgaa gaaggaactt
     4921 attaataaaa agtgaaaaga ctactggaag tttattttga taaaaaatcc agtagatctt
     4981 ttcataataa aacgcattgt atcaagtgtt aggagctgat atcatgcgtt tttctgcttt
     5041 taagattttt tccaactctg tttgtaagcg catcataaca aagggtctag gattcagggc
     5101 tctcctccta tatactatta gtaaagtaaa actaagggag gatattttag tgtcgcagtc
     5161 tattgttcct gtagagattc cacaatattg tcgttttgat tctaaaaaga gaaatggaat
     5221 tctgtttaac gttcgtattg ccaatcttaa atttaccttt tttagattag acgtcctgcg
     5281 aaacaaaata tggtatagta gttctatgaa tgatgaagca agtaaacaac taactgatgc
     5341 atgatttaag cgtcttgttg gtgttcagcg taccactttt gaagagatgt tagctgtatt
     5401 aaaaacagct tatcaactta aacacgcaaa aggtggacga aaacctaaat tatgcctaga
     5461 agaccttctt atggccactc ttcaatatgt gcgagaatac cgcacttatg aagaaattgc
     5521 ggctgatttt ggtatccacg aaagcaactt aatccgtcgg agccaatggg ttgaagtaac
     5581 tcttgttcaa aatggtttta cgatttcaag aactcctctc agttctgagg acacggtaat
     5641 gattgatgcg acggaagtac aaatcaatcg ccctaaaaaa acaattagcg aatgattctg
     5701 gtaaaaagaa atttcacgct atgaaggctc aagcgattgt cacaagtcaa ggaagaattg
     5761 tttctttgga tatcactgtg aactattgtc atgatatgaa gttgttcaaa atgagtcgca
     5821 gaaatatcgg acaagctggt aaaatcttgg ctgacagtgg ttatcaaggg ctcatgaaga
     5881 tatatcctca agcacaaact ccacgtaaat ccagcaaact caagccgcta acagttgaag
     5941 ataaagcctg taaccatgcg ctatccaagg agagaagcaa ggttgagaac atctttgcca
     6001 aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat
     6061 tacgaatgaa tttgagtgct ggtattatca atcatgaact aggattctag ttttgcagga
     6121 agtctattga ggtattgagc tagtttatga aaaaattggg tgaaaagtcg agtgttttag
     6181 aaacccacag tgtagtattc tagtttcaat ccactatatt ttgctactcc ccgtaaagtt
     6241 tctattttcc ctgatttctg atataataga aatattgact tcaagagtaa ggaagagaag
     6301 atgaacgcat tattaaatgg aatgaatgac cgtcaggctg aggcggtgca aacgacagaa
     6361 ggtcccttgc taatcatggc aggggctggt tctggaaaga ctcgtgtttt gacccaccgt
     6421 atcgcttatt tgattgatga aaagctggtc aatccttgga atatcttggc cattaccttt
     6481 accaacaagg ctgcgcgtga gatgaaagag cgtgcttata gcctcaatcc agcgactcag
     6541 gactgtctga ttgcgacctt ccactccatg tgtgtgcgta ttttgcgtcg cgatgcggac
     6601 catattggct acaatcgtaa ttttacaatt gtggatcctg gtgaacagcg aacgctcatg
     6661 aaacgtattc tcaaacagtt gaacttggac cctaaaaaat ggaatgaacg aactattttg
     6721 gggaccattt ccaatgctaa gaatgatttg attgatgatg ttgcttatgc tgcccaagct
     6781 ggcgatatgt atacgcaaat tgtggcccag tgttatacag cctatcaaaa agaacttcgt
     6841 cagtctgaat ccgttgactt tgatgatttg attatgctga ccttgcgtct ctttgatcaa
     6901 aatcctgatg ttttgaccta ctaccagcaa aaattccaat acatccacgt tgatgagtac
     6961 caagatacca accacgctca gtaccaattg gtcaaactct tggcttcccg ttttaaaaat
     7021 atctgtgtgg ttggggatgc ggaccagtct atctacggtt ggcgtggtgc tgatatgcag
     7081 aatatcttgg actttgaaaa ggattacccc aaagccaagg ttgttttgtt ggaggaaaat
     7141 taccgctcaa ccaaaaccat tctccaagcg gccaatgagg ttattaaaaa taataaaaat
     7201 cgccgtccta aaaatctctg gactcaaaac gctgatgggg agcaaatcgt ttactatcgt
     7261 gccgatgatg agctggatga ggctgtattt gtagccagaa ccatcgatga acttagtcgc
     7321 agtcaaaact tccttcataa ggattttgca gttctctatc ggactaatgc ccagtcccgt
     7381 acaattgagg aagccctgct caagtctaac attccttata ccatggttgg cggaaccaaa
     7441 ttctacagcc gtaaggaaat tcgcgatatt attgcttatc tcaaccttat tgctaatttg
     7501 agtgacaata ttagttttga gcgtattatc aacgagccta aacgtggaat tggtctaggt
     7561 acagttgaga aaatccgtga ttttgcaaat ttgcaaaata tgtctatgct ggatgcttct
     7621 gctaatatta tgttgtctgg tatcaagggt aaggcagccc aatctatctg ggattttgcc
     7681 aatatgatgc ttgatttgcg ggagcagcta gaccacttaa gcattacaga gttggttgag
     7741 tccgtcctag aaaaaacagg ttatgtcgat attcttaacg cccaagcgac tctagaaagc
     7801 aaggcacggg ttgaaaatat cgaagagttt ctttctgtta cgaagaactt tgatgacacc
     7861 acggatgtga cagaagagga aactggtctg gacaaactga gtcgtttctt aaatgacttg
     7921 gctttgattg ccgacacaga ttcaggtagt caggagacat cagaagtgac cttgatgacc
     7981 ctgcatgctg ccaaaggtct cgaatttcca gttgtctttt tgattgggat ggaagaaaat
     8041 gtctttccac ttagtcgtgc gactgaagat ccagatgaat tagaagaaga gcgccgtcta
     8101 gcctatgtag gtatcacgcg tgcagagaaa attctctatc tgaccaatgc caactcacgc
     8161 ttgctttttg gtcgtaccaa ttataaccgt ccgactcgtt ttattaacga aatcagttca
     8221 gacttgcttg agtatcaagg tctggctcgt cctgcaaata caagctttaa ggcatcatat
     8281 agcagtggta gtatttcctt tggtcaaggt atgagtttgg ctcaggctct tcaagaccgt
     8341 aaacgcggtg ctgccccaaa atcaatccag tcaagcggtc ttccatttgg tcaatttaca
     8401 gctggcgcaa aaccagcatc tagcgaggca aattggtcca ttggtgatat tgctctccac
     8461 aagaaatggg gagagggaac cgttctggaa gtttcaggta gcggtgctag gcaggaattg
     8521 aaaatcaatt tcccagaagt aggtttgaaa aaacttttag ccagtgtggc tccaattgag
     8581 aaaaaaatct aattttccat ccttctcacg aataataaag tgaggaggat ttttatgtac
     8641 agtatttcat tccaagaaga ttcactatta ccaagagaaa ggctggccaa ggaaggagtt
     8701 gaagcgctta gtaaccaaga gttgctagct attttactca ggacaggaac acgtcaagct
     8761 agcgtttttg aaattgccca aaaagtcttg aacaatcttt caagcctaac ggatttgaaa
     8821 aaaatgaccc tgcaggaatt gcagagtttg tctggtattg ggcgtgttaa ggccatagaa
     8881 ttacaagcta tgattgaact ggggcatcgt attcacaaac acgagactct tgaaatggaa
     8941 agtattctca gcagtcaaaa gttggccaag aagatgcagc aggaattagg ggataaaaaa
     9001 caagagcacc tggtggcact ctatctcaat actcaaaatc aaatcatcca tcagcagacc
     9061 atttttatcg gttctgtaac tcgtagtatc gctgaaccgc gagagattct tcactatgca
     9121 atcaagcata tggcgacttc tcttatcttg gtccacaatc atccttcagg agcggtagcg
     9181 cctagccaaa atgatgatca tgtcactaaa cttgttaaag aagcctgcga actgatgggg
     9241 attgttctct tggaccattt gattgtctct cattctaatt actttagtta tcgtgaaaag
     9301 acagatttaa tctaaagttc attaacgaca tagtcaaaga gtttcttatc tttgggacga
     9361 ttttcaaaaa gaagttctgg atgccattgg acaccgagaa aggcgacatc atccgtactc
     9421 atgacagcct caatgatacc atctttagga tcatgagcca caacttttaa atttggtgct
     9481 aagtccttga tgctctggtg gtggaaggag ttgatatgag agatttctcc atagatttct
     9541 tggagaacgg tatctggttc tgttaccaag cgttgagttg tgtactcaac agaagaatcc
     9601 tgccaatggt cttcgatatc ttggtacaaa gttccaccca tggcaacgtt aaagagttgg
     9661 gtaccacggc agacagagaa aatgggcttt ttctgtttaa tagcttcctt gatgagggcc
     9721 agttcgaaga tatctctttg aaggtgataa tcatcgctat caatggtttt gggttcgcca
     9781 tagaattttg gatcgacatt ttgcccacct gtcaagatga gcttgtcaat caaactgata
     9841 tagtggcagg ccatttcttg atcaccaatc ggtaggatga tgggaatccc tccagcatct
     9901 ttaacgcctt caacaaagcc ttttgctgcg tagctcatca tgatgtcatc atctggatga
     9961 gttttttcgt ttcctgtaat cccaataact ggttttttca taaaatgatt ttcgctttct
    10021 aatcctcttt tcgcatgaag tagaggaggg tttggagttc acttgtcaaa tcgacatact
    10081 gaacgaccac gtcttttggt aaatgcagat ggactggtga aaaactgaga attcctttca
    10141 caccagcatc aaccaagaga ttagcaacct cttgtgactt gacgctggga acagttagga
    10201 tagcagtctt cacatcagca tccttgattt tatccttgat ctgagaaatc ccgtaaatgg
    10261 gaatcccgtc aggagtttgg gtaccgactt caggatggtc gtctaggtca aaggccatga
    10321 taatcttcat cttgttacgt tcgtggaagc ggtagtggag aagggcatgg cccatatttc
    10381 caataccaac cagcatgaca ttggtaatag agttgtcatt gagcaaatcg gcaaaaaatg
    10441 tcattagttt tttgacatca tagccaaaac cacgacgacc aagttcacca aaataggaaa
    10501 aatcacgacg tacggtcgct gaatcaatac cgatagcctc tgcaatttgc ttagagttgg
    10561 cacgttcaat cttttctgca tgaaatctct taaaaattcg atagtagaga gagagtcttt
    10621 ttgctgtagc ttttggaata gcaaactgtt tatctttcac aaaatcacaa cctttctat
//
LOCUS       DAVLVZ010000060        10589 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000060 DAVLVZ010000000
VERSION     DAVLVZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10589
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.060"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..1762
                     /locus_tag="VYN77_001617"
     CDS             <1..1762
                     /locus_tag="VYN77_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1559055.1"
                     /translation="VLLAKNYGTESLFKALEGYRKVFLPTISNNEWFKKQTKAYIVEE
                     KSTIEEGREKQGKEGTKYSIGGYDRLTNPSWKYQSMVLPLLTLPEEKTVFMIANISTI
                     GFGAYDRYRSSEYPKGEKLNKFVEDNAKEAAKRFRDHYDYWYKILDNDNKEKLYRSIL
                     VYDAFKFGTDKDKDKVTHQATFETDHPAIKYFFGPAGNNVVHNGHGAYATGDAFYYMA
                     YRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRSGLGPEFYAKGLLQAPDHPYDPTITI
                     NSVLKYEDSENSTRLQVADPTQRFNSAEDLHNYMHNMFDVIYMLEYLEGKAVANLETN
                     QKYELLRKIENKFDLDQDGNNVYATNVVRRLTMDEVNKLNSFDSLIENDIITSRGYKD
                     QEYKRNGYYTIDLFSPIYSALSGEKGTPGDLMGRRIAFELLAAKGYKEGMVPYISNQY
                     EKDAKAVGSKINSYGKEVGLVTDELVLEKVFNGQYKTWTQFKKDMYKEREKQFSKLNR
                     VNFINPNNPLSRQRNVSVTDIGVLERMIVEAVRDDAQDDVAKFYPETNSRVLKLKKAI
                     YKAYLDQTNDFRSSIFENKK"
     gene            1806..7004
                     /locus_tag="VYN77_001618"
     CDS             1806..7004
                     /locus_tag="VYN77_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1559056.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVIGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVQPTLPEAVVTDKGEPEVHEKPDYTQPIGAN
                     LVEPEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEPISTVSEVASEREELPSL
                     HTDIRTETIPKTTIEESDPSKFIGDDSVKEVGEDGERQIVTSYEELHGKKISEPVETV
                     TILKEMKPKILVKGTKENPKEKTVPVLTLTKVTEDAMNRSANLNYELDNKDNAEISSI
                     IAEIKDGDTVVKKVDLSKEKLTDAVQNLDLFKDYKIATTMIYDRGQGSETSKLDEKTL
                     RLELKKVEIKNISSTNLVKVNDDGTEIPSDFMSEKPSDEDVKKMYLKITSRDNKVTRL
                     AVDKIELVTEKEKELYKITASAQDLIQHVDPSKTRNEYIHYIEKPVPKVNNVYYNFNE
                     LVRDMQEHPNDEFKLGADLNATNVSAFGKSYVTKDFKGKLLSDGDNHYTIHNLSRPLF
                     GNVIGGTIKNINLGNVDINMPWANQVAAVANIIKGGTTIENVKVKGNIVGKDWVSGFI
                     DKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVERAYVNANLIGKKAKAAG
                     IAYWTQNEGNNNTVRQEGAIKKSIAKGTIQVTEAIESGGVVGSMKHHGSVEDSVSMMK
                     VPNGEIFYGSSDIDYDDGYWTGDNVRRNYVVIGVSDGHSSYQRSKDKNRIRPISEEEA
                     KSKIEATGITADKYEINEPVVNRLNRLTRREDEYKSTQDYKVDRDLAYRNIEKLQPFY
                     NKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDHVMIHYADKTKEIKAV
                     HQKESKVAQVREYSIDGLDDIVYTPNMVDKNRDQLIKDIKDRLATVELISPEVRALMD
                     KRDTSRDPNANSDERKNGYIRDLYFEESFSETKANLDKLVKSLIENADHQLNSDEAAM
                     KALVKKVDENKAKIVMALTYLNRYYDIKYGDMTIKNLMMFKPDFYGKSVDLLDFLIRI
                     GSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFTNDTDLNDWFIHAAKNVY
                     IVEPKTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAHMFLISTYNTMAYSSFEK
                     YGKYTEAEREAFKDKIKEVAHAQQTYLDFWSRLALPSVRDQLLKSQNRVPTPVWDNQN
                     YHNVEGVNRMGYDKNNKPIAPIRELYGPTWRYHTTNWYMGAMASIFQDPNNNDQVYFM
                     GTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAYAQGMLQTPDKSGNGEYG
                     ALGLNMAYHRENDGDQWYNYDPDKLKTREDIDRYMRNYNDALMMLDHLEADAVIPKLH
                     GNISRWFKKMDRQYRKNGELHQFDKVRELTEDEKKKIVINNIDDLVNNNLMTKHGAPS
                     DRTYNPEDFDSAYVNINIMTGIYGGNTSQGAPGAASFKHNTFRMWGYFGYENGFISYA
                     SSKYQGEADKTNKKLLGDDFIIKKVSKDKFNNLEEWKKQYFKDVKSKAEKGFTAIEID
                     GRQITNYAQLKTLFAEAVQKDIDGMSDPKIKDHFKNTVDLKSKVFKALLKNTDGFFNK
                     LFKEDI"
     gene            7250..8101
                     /gene="ylqF"
                     /locus_tag="VYN77_001619"
     CDS             7250..8101
                     /gene="ylqF"
                     /locus_tag="VYN77_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1559057.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWCQYFESQEIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            8088..8867
                     /locus_tag="VYN77_001620"
     CDS             8088..8867
                     /locus_tag="VYN77_001620"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1559058.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            8883..10433
                     /locus_tag="VYN77_001621"
     CDS             8883..10433
                     /locus_tag="VYN77_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1559059.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGISKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSIEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3664 a         1687 c         2141 g         3097 t
ORIGIN      
        1 tgtattatta gcgaaaaatt atggaactga aagtttattt aaagcattag aaggatatag
       61 aaaagtattc ttaccaacaa tttctaacaa tgagtggttt aaaaaacaaa caaaagcata
      121 tattgtagaa gaaaaatcta caattgaaga gggaagagaa aaacaaggaa aagagggaac
      181 taaatattct attgggggat atgatagatt aactaatcca tcttggaaat atcaaagtat
      241 ggtcttacca ctattaactt tacctgaaga aaaaacagtg tttatgatag cgaatatttc
      301 tactattgga tttggagcct atgacagata tagaagttca gaatatccta agggagaaaa
      361 gttaaacaaa tttgtagaag ataacgccaa agaagcggct aaacgattta gagatcatta
      421 tgattattgg tataagatat tagataacga taataaagaa aaattatata gaagtattct
      481 agtatatgat gctttcaaat ttggtactga caaagataaa gacaaagtaa ctcatcaagc
      541 tacttttgaa actgatcatc cagctataaa atacttcttt ggacctgcgg gaaataatgt
      601 tgttcataat ggacacggag catatgctac cggagatgca ttctattata tggcatatcg
      661 tatgttggat aaagatggag ctgtaacata tactcatgaa atgacacata actcagatag
      721 agagatttat cttggaggat atggaagaag aagcggatta ggacctgagt tttatgcaaa
      781 aggattatta caagctccgg atcatccata tgatccaaca ataacaatta actctgtctt
      841 gaaatatgag gattcagaaa attcaacaag acttcaagta gctgatccaa ctcaacgatt
      901 taatagtgca gaagacctac ataattatat gcataatatg tttgatgtaa tttatatgtt
      961 agaatattta gaaggaaaag ctgtagctaa tttagaaaca aatcaaaaat atgagttact
     1021 aagaaaaatt gaaaataaat ttgatcttga tcaagacggt aataatgtat acgcgacaaa
     1081 tgttgttcgc agattaacga tggatgaagt taataaatta aattcttttg atagtttaat
     1141 tgaaaatgat attattacta gcagagggta caaagaccaa gaatataaga gaaatggtta
     1201 ttatacaatt gatttatttt caccgattta ttcagctctt agtggagaga aagggactcc
     1261 tggtgattta atgggacgcc gtatagcatt cgaattatta gctgcaaaag gatataaaga
     1321 gggcatggta ccttatatct caaatcaata tgaaaaagat gcaaaagcgg tgggaagtaa
     1381 gattaattct tatggtaaag aagtaggttt agttacagat gaattagtac ttgaaaaagt
     1441 atttaatggt caatataaaa cttggacaca attcaagaag gatatgtata aggaaagaga
     1501 aaaacaattc agtaaactaa atagagttaa ctttattaat cctaataatc cattgagtcg
     1561 tcaacgaaat gtatcggtta cggatatagg tgtactagag agaatgattg tcgaagcagt
     1621 gagagatgat gcgcaagatg acgtagcaaa attttaccca gaaacaaata gtagagttct
     1681 taaactgaaa aaagcaatat ataaagctta tcttgaccaa acaaatgatt ttagaagttc
     1741 aatttttgag aataaaaaat agtgtttact attaggaaat aaaaagtgaa tgtggaggat
     1801 tagaattgtc attattaaaa aaagataaat tttccattcg gaaaataaag ggtattgttg
     1861 gttcggtatt cttggggagt cttttatttg caccgtcagt tattggtgca tcgacttatc
     1921 attacttaga ttatagtagt ttgacacaaa ctgaacgtga tcaactcaaa caaggtagac
     1981 ctgatgaatc caaagagtca tatgctttgg attatgaaaa agacgcacta ccaaacacag
     2041 gcagttctca atctattatg actgcattgg gtttgttagc tatcgggagt cttattgtga
     2101 ttatcacaaa agataataga aataaaaaaa tagctacatt tttgattgta ggggcgacag
     2161 gtttagttac actatcaact gcttcagcac ttaatttgaa tgctaacatc catgagtctg
     2221 gacgtgatgg tgtgttgcaa atttctggtt acagatatgt tggttacttg gagcttgatg
     2281 ataaaactgt ttcgtcggtt tctccagcat caactgtttc gccagttgag caacccaaag
     2341 ttgtaacaga gaaaggggaa ccggaagtac aaccgacatt accagaagct gttgtgactg
     2401 acaaaggcga accagaagtt catgagaagc ctgactatac gcaaccaata ggtgcaaacc
     2461 ttgttgagcc tgaagttcat gagaaactag catacactga accagtaggt acaacaggag
     2521 tggatgaaaa tggaaacttg attgaaccac ctgttaacga cattccagag tatactgaac
     2581 caatatctac agtttcagaa gttgcatcag aaagagaaga gttgccttct ttacataccg
     2641 atattcgtac agagactatt cctaaaacca ctatagaaga atctgatcct agcaaattta
     2701 taggcgatga ttctgttaaa gaagttggtg aggatggcga acgtcaaatt gttactagct
     2761 atgaagagtt acatggcaaa aaaattagtg aaccagttga aacggtaaca attttgaaag
     2821 aaatgaaacc taaaattctt gtaaaaggta caaaagaaaa tccaaaagaa aaaacagttc
     2881 ccgttttgac cttgaccaaa gtaacagaag atgctatgaa tcgttctgcc aacttaaatt
     2941 atgagcttga taataaagat aatgctgaaa tatcaagtat tatagctgaa attaaagatg
     3001 gtgatacagt agttaaaaag gttgatttat ctaaagaaaa attaactgac gctgtacaaa
     3061 atttggattt gtttaaagat tataagattg caacaacaat gatttatgat cgtggtcaag
     3121 gttctgaaac gtctaaactg gatgaaaaaa ctttgcgttt ggagttgaaa aaggtcgaaa
     3181 ttaaaaatat ttcatctact aatttagtga aggtaaatga tgatggtact gaaataccta
     3241 gtgatttcat gagcgaaaaa ccttctgatg aagatgtgaa gaaaatgtac ttaaaaatca
     3301 ctagtcgtga taataaagta actcgtttag cagttgataa gattgagtta gtaactgaaa
     3361 aagaaaaaga actttataaa attacagcaa gcgctcaaga tttaattcaa catgtcgatc
     3421 catctaagac tagaaatgaa tacattcatt acattgaaaa accagttcca aaagtcaata
     3481 atgtatatta caatttcaat gaacttgtga gagatatgca agaacatcca aatgacgagt
     3541 ttaaattggg tgctgacctt aatgccacaa atgtatcagc atttggtaaa tcatatgtta
     3601 cgaaagattt caaaggtaag ctcttgagtg atggtgataa tcactatacc attcataact
     3661 tgtcacgtcc gttatttggc aatgttattg gtggaacgat taagaatatt aatcttggta
     3721 atgttgacat aaatatgcca tgggcgaacc aagttgcagc tgtagcgaat attattaaag
     3781 gtggaactac cattgaaaat gttaaggtta aaggtaatat agtaggtaaa gattgggtat
     3841 ctggatttat tgataagatt gataaccaag gtacactcag aaatgtggcc tttatcggta
     3901 acgtaacttc tgtcggcgat ggtggacaat tcttgactgg tatcgttggt gagaactgga
     3961 aaggactcgt cgaacgcgcg tatgttaacg caaatcttat tggcaagaaa gccaaggctg
     4021 cgggcattgc ttattggaca caaaatgaag gaaataacaa tactgttcgt caggaaggag
     4081 caatcaaaaa atctattgct aagggaacaa ttcaagtaac tgaggcgatt gaaagtggtg
     4141 gtgttgtagg aagcatgaag caccacggtt ctgtcgaaga ttcagtttcc atgatgaagg
     4201 tacctaatgg tgaaattttc tatggttcta gtgatattga ttatgatgat ggctactgga
     4261 caggagataa tgtaagacga aattatgttg taataggtgt tagtgatggg cattctagct
     4321 accagcgttc aaaagataaa aatcgtatca gacctatttc tgaggaagag gcaaaatcta
     4381 aaattgaagc tacaggaatt accgcagata aatacgaaat caatgaacct gtggtcaatc
     4441 gcttaaatcg tttaaccaga agagaagatg aatataagtc aacccaagac tataaagtag
     4501 atcgagatct ggcttatcgt aatattgaga aactgcaacc attttataac aaagaatgga
     4561 ttgtcaatca aggtaacaaa cttgcagaag attcaaactt agctaagaaa gaagtccttt
     4621 ctgttacggg tatgaaggat ggtcaattcg tgaccgattt atcagatatt gatcatgtga
     4681 tgattcacta tgcagataag actaaagaaa tcaaagctgt tcatcaaaaa gagtctaaag
     4741 tagcgcaagt tcgtgagtat agtatcgatg gattagatga cattgtttat acaccaaaca
     4801 tggttgataa aaatcgtgat caattgatta aagacattaa agataggtta gcaactgttg
     4861 aattaatttc tcccgaagtc cgtgctttga tggataaacg agatacatct agagatccta
     4921 atgctaattc ggatgagcgt aagaatggtt atatccgaga cctttatttc gaagaaagtt
     4981 tttctgaaac gaaagctaat cttgataaat tggtcaaatc tttgattgaa aatgctgacc
     5041 atcaattaaa tagtgacgaa gcagctatga aagcacttgt taagaaggtg gatgaaaata
     5101 aagctaaaat cgtgatggct cttacttacc ttaatcgtta ctatgatata aagtatgggg
     5161 atatgactat caaaaacttg atgatgttta aacctgattt ctatggtaag tcagttgacc
     5221 ttcttgactt cttgattcga attggatcaa gtgagcgaaa tatcaagggt gacagaactt
     5281 tggatgctta tcgtgatatg attggtggaa ctattggtaa gtctgaatta catggcttct
     5341 tagattacaa catgcgtctc ttcactaacg atactgattt aaatgattgg tttatccatg
     5401 cggctaagaa cgtatacatt gtggagccta aaacaacgaa cccagatttt gttaataaac
     5461 gacatcgtgc atttgatggt ttgaataatg gtgttcataa tcgtatgatc ctacctctat
     5521 taactcttaa gaatgcacat atgttcttga tttcgactta caatacaatg gcttatagct
     5581 cttttgaaaa atatggtaag tatacagaag ctgaacgaga agcatttaaa gataaaatta
     5641 aagaagttgc tcacgcacaa caaacttatc ttgacttctg gtcgcgttta gcattgccaa
     5701 gcgtacgtga ccaattactc aagagtcaaa atagggttcc aacacctgtt tgggataacc
     5761 aaaactatca taacgtcgaa ggtgtaaatc gtatgggtta tgataagaat aataaaccaa
     5821 ttgcacctat tcgtgaatta tatggaccta cttggcgtta tcatactaca aattggtaca
     5881 tgggagctat ggcatctatt ttccaagatc ccaataataa tgaccaagtt tatttcatgg
     5941 gaacaaatat gattagtccg tttggtattt cagcctttac tcacgaaaca acacacgtaa
     6001 acgatagaat gctttacttc ggtgggcata gacaccgtca aggtacagac gttgaagcct
     6061 atgcgcaagg tatgttgcag acacctgata aatctggtaa cggtgaatat ggtgctcttg
     6121 gtcttaacat ggcttatcat cgtgaaaatg acggtgacca atggtataat tatgatccag
     6181 ataaattgaa aactcgtgaa gatatcgacc gttatatgag aaattataat gatgctctta
     6241 tgatgttaga ccatcttgag gctgatgctg ttatccctaa attacatggt aatattagtc
     6301 gatggttcaa gaaaatggat agacaatatc gcaaaaatgg agagctacat caatttgata
     6361 aagttcgtga attaactgaa gatgagaaga agaaaattgt tatcaacaac attgatgatt
     6421 tagtcaacaa taatttgatg acaaaacatg gcgctcctag tgacagaaca tataatccag
     6481 aagattttga tagtgcttac gtaaatatta atataatgac cggtatctat ggtggtaata
     6541 ctagccaagg agcacctgga gctgcatcat tcaagcataa tactttccgt atgtggggct
     6601 attttggtta tgaaaatgga ttcattagtt atgcatctag caaatatcaa ggcgaagctg
     6661 acaaaaccaa taaaaaatta ttgggtgatg atttcattat caagaaagta tctaaggata
     6721 aattcaataa tcttgaagag tggaagaaac aatacttcaa agatgttaag tcaaaagcag
     6781 aaaaaggatt tactgctatt gaaattgatg gtcgtcaaat tacaaattat gctcagttga
     6841 aaactttatt tgctgaagct gttcaaaaag atattgatgg tatgtcagat ccgaaaatta
     6901 aagatcattt caaaaataca gtagacttga aatccaaagt attcaaagcc cttcttaaaa
     6961 acacagacgg tttctttaat aaactgttta aagaagacat ttaaatgttt aaaatataaa
     7021 gaggacagtt ttttgctccc tctgaaaagt catcatttga tggctttttt ctatataggg
     7081 taaaagatag ggtaaaaggc tatcatcgga caaaataaag aaggcatgat ataatataaa
     7141 gtagattttt atatcataaa acaagaactg tttggacatc attcattttt agattgagat
     7201 ctcttagatc tagattaaca gcttcagagc tacgaaagga aaaaactcaa tggctactat
     7261 tcaatggttt cctggtcaca tgtctaaagc gcgtcgacag gtgcaggaaa atttaaaatt
     7321 tgttgatttt gtgacgattt tagtagatgc acgcttgcct ctatctagtc aaaatcctat
     7381 gttgaccaag attgttggtg ataaaccaaa actcttgatt ttaaacaagg ccgacttggc
     7441 tgatccagca atgaccaagg aatggtgtca gtattttgaa tcacaagaaa tccagacgct
     7501 agctatcaac tccaaagagc aagtgactgt aaaagttgta acagatgcgg ccaagaagct
     7561 catggctgat aagattgctc gccagaaaga acgtgggatt cagattgaaa ccttgcgtac
     7621 catgattatc gggattccaa acgctggtaa atccactctg atgaaccgtt tggctggtaa
     7681 aaagattgct gttgttggaa acaagccagg ggtcacaaaa ggtcaacaat ggcttaaaac
     7741 caataaagat ctggaaatct tggatacacc ggggattctc tggcctaagt ttgaggatga
     7801 aactgttgca cttaagttgg cattgactgg agctatcaag gatcagttgc ttcctatgga
     7861 tgaggttacc atttttggta tcaattattt caaagaacat tatccagaaa agctggctga
     7921 acgcttcaaa caaatgaaaa ttgaagaaga agcgcctgtg attattatgg atatgacccg
     7981 cgccctcggt ttccgtgatg actatgaccg tttttacagt ctcttcgtga aggaagtccg
     8041 tgatggcaaa ctcggtaact ataccttaga tacattggaa gacctcgatg gcaacgatta
     8101 aagaaatcaa agaactcctt gtgacagtca aggagttaga aagccctatt tttttagagc
     8161 ttgaaaagga taatcgctca ggagttcaaa aggaaatcag caagcgtaaa agagccattc
     8221 aagctgaatt agatgaaaat ttgcgcttgg aatccatgct ttcttatgaa aaagaacttt
     8281 ataagcaagg attgacctta attgtaggta ttgatgaggt tggtcgtggt cctcttgctg
     8341 gtcctgtagt cgctgcggcc gtcattttac ctaaaaattg taagattaaa ggtctcaacg
     8401 acagcaagaa aattcctaaa aagaaacatc tggagatttt ccaagccgtt caagaccaag
     8461 ccttgtcgat tggaattggt atcatagata atcaggtcat cgaccaagtc aacatctatg
     8521 aagcaaccaa actagccatg caagaagcaa tctcccagct cagccctcaa ccagagcacc
     8581 ttttgattga tgccatgaaa ctggacttgc ccatttcaca aacctccatt atcaaaggag
     8641 atgccaactc cctctctatc gcagcagcat ctatagtagc caaggtaaca cgtgatgaat
     8701 tgatgaaaga atacgatcag cagttccctg gctatgattt cgctactaat gcaggatatg
     8761 gcacagctaa acatctggaa ggcctcacaa aactaggagt taccccaatt caccgaacca
     8821 gctttgaacc cgttaaatca ctggttttag gtaaaaaaga aagttaattg aaaggaaata
     8881 ccatggagga acagtcggaa atagtccgtt ctaagaaaga attcgccttt gcatccagca
     8941 ctatactatc ccaagttggt cgaggaatca ttgtcggcct catcgttgga attatcgtcg
     9001 gatcctttcg tttcttaatt gaaaagggct tccacctgat acaaggagtt tatcaagatc
     9061 aagggtactt agtgcgcaat ctttttgtac tggttttgtt ttatatactc atctgttggc
     9121 tcagtgccaa actaacacgg tcagaaaaag atattaaagg ctcaggaatt cctcaagtcg
     9181 aagccgaact gaaaggcctc atgtccctca actggtgggg cattctttgg aaaaaatatg
     9241 tgctaggtat tcttgctatt gccagtggac tcatgctggg tcgagaggga cccagtattc
     9301 aacttggagc agttggtggt aaaggaattt ccaagtggct caaatccagt ccagtagagg
     9361 aacgttcctt gattgccagt ggagctgcag caggtttagc cgcagccttt aatgctccta
     9421 ttgcagcact tctctttgtt gtagaagaag tctatcacca tttttcgcgc tttttctggg
     9481 tctcaactct agcagccagc atcgtagcaa actttgtgtc tctactcatg ttcggtttga
     9541 caccagtatt ggatatgcca gataacattc ctcccatgac cctagatcag tattggatat
     9601 atctcgtcat gggaattttc cttggatttt caggctttct ctatgagaaa gctgtattaa
     9661 acgttggaag agtttatgac ttgattggtc aaaaaatcca tttggataga gcttattatc
     9721 ccatcttggc ttttatcctt atcataccag tcggaatctt cttacctcaa atcattggtg
     9781 gcggaaatca gcttgtcctt tctttaactg aacaaaattt tagtttccaa gttttattag
     9841 cttacttttt gatccgcttt atttggagta tgattagcta tggaagtgga ctgccaggag
     9901 gaattttcct ccccatttta gctcttggtt ctttgcttgg tgccttaatt ggtgttatct
     9961 gtgtcaatct tggacttgtc agtcaagagc aattccctat atttgtcatt ctaggaatga
    10021 gtggctattt tggagccata tcaaaagctc ccttaaccgc tatgatcctc gtaactgaga
    10081 tggtaggaga tattcgcaac ctcatgccac ttggtcttgt cactcttgtt tcttatatta
    10141 tcatggattt gctcaaaggt acgccagtct atgaagccat gctggaaaaa atgcttccag
    10201 aagaagtatc tatcgaagga gaagttacac ttatcgaaat accagtttct gataaaattg
    10261 ctgggaaaca agttcatgaa ctcaacttac cacacaacgt cctcatcaca actcaagtcc
    10321 ataatggcaa gagccaaaca gttaacggct caaccagaat gtatctgggt gatatgattc
    10381 acctggttat tccaaaaagt gaaattggaa aagtcaaaga tttgttgttg tagcatgagt
    10441 atttacataa tttatgttat gtaaatactc ggcttgattt atttagaaaa ccgattctca
    10501 gaaatgagat cggttatttt ttactgatga ggaattttac atataaataa ttgaacttta
    10561 ttaaaaataa gactataatt aagttagaa
//
LOCUS       DAVLVZ010000061        10367 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000061 DAVLVZ010000000
VERSION     DAVLVZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.061"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(7..1221)
                     /locus_tag="VYN77_001622"
     CDS             complement(7..1221)
                     /locus_tag="VYN77_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1559060.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1377..1670)
                     /locus_tag="VYN77_001623"
     CDS             complement(1377..1670)
                     /locus_tag="VYN77_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559061.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            1832..2284
                     /locus_tag="VYN77_001624"
     CDS             1832..2284
                     /locus_tag="VYN77_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1559062.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIVHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2319..3707)
                     /locus_tag="VYN77_001625"
     CDS             complement(2319..3707)
                     /locus_tag="VYN77_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1559063.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3773..4735
                     /locus_tag="VYN77_001626"
     CDS             3773..4735
                     /locus_tag="VYN77_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1559064.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4732..4923)
                     /locus_tag="VYN77_001627"
     CDS             complement(4732..4923)
                     /locus_tag="VYN77_001627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559065.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDETDEVSSKHVFEVVDETDEVS
                     NHTYGKATLTWFAEIFEEY"
     gene            complement(4987..5997)
                     /gene="ccpA"
                     /locus_tag="VYN77_001628"
     CDS             complement(4987..5997)
                     /gene="ccpA"
                     /locus_tag="VYN77_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1559066.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6422..7021)
                     /locus_tag="VYN77_001629"
     CDS             complement(6422..7021)
                     /locus_tag="VYN77_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1559067.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDITILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7023..8120)
                     /locus_tag="VYN77_001630"
     CDS             complement(7023..8120)
                     /locus_tag="VYN77_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1559068.1"
                     /translation="MLERLKSIHYMFWASLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGLMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(8120..8857)
                     /locus_tag="VYN77_001631"
     CDS             complement(8120..8857)
                     /locus_tag="VYN77_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1559069.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(8859..9743)
                     /locus_tag="VYN77_001632"
     CDS             complement(8859..9743)
                     /locus_tag="VYN77_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559070.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(9730..9924)
                     /locus_tag="VYN77_001633"
     CDS             complement(9730..9924)
                     /locus_tag="VYN77_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559071.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            complement(9921..10127)
                     /locus_tag="VYN77_001634"
     CDS             complement(9921..10127)
                     /locus_tag="VYN77_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559072.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         3151 a         2377 c         1880 g         2959 t
ORIGIN      
        1 caagccctat ctacgatact gtttcaagaa acgagtcatc ttttcttgga tttgggctaa
       61 ctcatcaaca cgaggaaggt aaacgatacg gaagtggtct ggttcctgcc agttaaagcc
      121 tcgaccatga accaagagaa ccttttcctg cttcaagaaa tcaaggacaa actgctcatc
      181 atcatcgata cggtacatat tgcggtcgat ttttgggaag atatagagcc ccgccttggg
      241 tttaacggca gacaaacctg gaatatcttg aatggcatta tagatgaaat ttctttgctc
      301 gtagattcgt ccaccaggaa gaagcaattc atcgactgat tggtgacccc ccaaggaagt
      361 ttgtacgact tgttgggcca aaacgttaga gcaaaggcgc atattggaca gcatattgag
      421 cccttcgata tagcccttaa catgagtctt agggccagac aagaccatcc aacccacacg
      481 gaaacctgct atgcggtggg attttgacag accattcatg ctgacacaga agacatctgg
      541 tgccaagctc gccacaggcg tatgcacatg tccgtccatt accatgcggt catagatttc
      601 atccgcaaag ataatcaaat cgttttgacg ggcaatctca ataatctcca gcaagagttc
      661 cttaggataa agggctccag ttgggttatt tggattgata aggacgattg ccttggtatt
      721 ggaagtgatt tttgacttga tatcgtcaat atctgggtac cattcaacag cttcatcaca
      781 gatatagtga acggcatttc ctccagctag gctgacagcc gccgtccata gaggatagtc
      841 tggcattggc actaagacct catccccatt gtccaaaagc ccctgcatgg acatgacaat
      901 caactcactg acaccatttc caaggtaaat atcatcaata tctacgtttg ggaatttctt
      961 cagctggcaa tactgcatga tggccttacg ggctgagaaa attcctttgg agtcagaata
     1021 gccttcacta tcacgcgcat tcataatcaa gtcatgaatg acctcgtctg gcgctgtaaa
     1081 gccaaattct gctggatttc ctgtattcag acgtaaaatc ttttctccat tcgctcgcat
     1141 ccgcatggct tcttccaata caggaccacg gatatcataa gcaacatgct ctaacttact
     1201 agacttgtta tattctttca tcttgtttcc tcaattcatc aggatagtaa cattatacca
     1261 ctagaaagag aatgcgacaa gtttactgaa atgtgttact agtttcgtat agaagaaaaa
     1321 ccttgctaaa ggcgaggtta agaacgtaga gatagcaggt attttgcaaa gacacatcac
     1381 ataccaaaag ataaacctag aaaaagcaag cctagaaagg agtcataaag aagattaaac
     1441 atgaggaaaa gcccccatat catcaagtaa tcccaacgcc cacttcgttt tgcaactaga
     1501 aataagatta gatggtagag tacatgaaaa actaaaaatc caataaaact tggataaaga
     1561 agcggtacca gactctctaa ttgacagatc atgatcactt ctaccaagac tgaaactagg
     1621 ataattccat aataaaggaa atttgatttt tgaaaagagt ttttcatcat tctcccttcc
     1681 ttcacttctt tcctgctatc ctttctttta ttttatcata tatttatata gagtttaagc
     1741 cctaatattc ttttgaaaaa tacttttttt gctttacttc tatagtgaaa aggtttacaa
     1801 ttatagtaag aaaatttcag gaggtttcat tatggcacaa cgttaccaaa atatcatggt
     1861 cgcaatcgat ggttctaagg aagcggactt ggcttttgtc aagggagttc attctgctct
     1921 acgaaacgac gctaaactca ccatcgtaca tgtcattgac acacgcgctc tccaaagcgt
     1981 atccaccttt gatgctgaag tttacgaaga actccaagtc gacgctgaaa gtctgatgaa
     2041 agagtacgaa aaacgtgcta aagatgctgg ggtagcagat gttcatatcg tcattgaaat
     2101 gggaaatcca aagaccctgc tagcacgtac tattccagat gccgaggaag tggacctcat
     2161 cctcgttggc gcaactggtc tcaacgcctt tgaacgcctc ttagtcggct cttcatctga
     2221 atacatactc cgccatgcta aggtcgattt gctggttgtg agagaacaag aaaaaacctt
     2281 ataatcacaa agaaaaggag cccctagctc ctttttgttt acgatttatt tctctcttta
     2341 tggcgttcgt aagccttgag ctggcgctgc agttcctttt taatagcagg ttctggagca
     2401 tatttttctt cccaattatc tggttttaag attttatggg tcactggatc aaaatgagcc
     2461 ttgccatctg gaaaaatttt ccccatattg gcctgatgga caatatcaaa aatacgttct
     2521 gggtccaccc ccatcaagac aaaactgccg taggtgaagt aaagcgtgtc aatcaaggca
     2581 tccacttgcc ctatcaaatc ttgctgagca ggtgtcttct tggctacttt acctgctgcc
     2641 ttatcaaggg cctgatgaag ttgcgataca gcttgaccaa aatcttcttc agaaggactg
     2701 gctgctcgaa caaactccac caattcttct attttaaaac cagccctatg ggttgcaccc
     2761 tctaaatccc aagctcgagg ttcttcttgg gttcgttcat ccatcatgtg gtggaaggtc
     2821 ttgaccttat tgaaatgata gtcacggctg acaaagactt tttctgaaga caaaacacca
     2881 aaatgttcca aggccttgtg gatcccatct tgttgattgc tagtggtaat gtgcttggca
     2941 gcttccttga cactgctact accatttccc atagcgaccg acataccgac gccagccagc
     3001 atttcaaggt cattatctga gtcaccaaaa gccatgactt ggttgaggtc aaagccatat
     3061 tctttcccaa ctcggcgaat gccttctaat ttagaatttc cctgattgat gacatccgat
     3121 gcaaaaggat tgctacgtgt caatttcaag tcttcaaaat cagctgccgc cttctcagat
     3181 tcttctggtg tcatcagcat caaaacttgg tagataggct gattcatcag gtgaagcagg
     3241 tcctcttcct tttggggaac aaccttgctg accatgcgat taaaagactg actcaccgtc
     3301 cgagttaaaa cagagggaac gaagcgacta attcgttggg aaaaagaacc cagaccaaag
     3361 gacatgattt tagaacccaa catggcatcc ttggtcccta gagcaatctc cgtgccctct
     3421 tttttagcat agctaattag atggcgcaaa tgtaacttgg aaatagggct cgtgaacaag
     3481 actctgtctt tactaaagat atactggcca ttataggtta ccgcaaaatc cagatccaaa
     3541 tcgtccatca attccttaac aaaaaaaggt cctcgccctg tcgctacgcc aactagtacc
     3601 ccttgttctt tgacaatctt aatcgcgtcc ttagtggatt tcaaaacact cttgcgattg
     3661 ttgaccaagg ttccatcgat atcaaaaaaa acagctttga cttccatcct attccaatct
     3721 ccccttttgt gatacaatga ttataccaca tttcagaaag agttagtaaa tcatgcctaa
     3781 gaaaatcctt gttttacata cgggtggaac tatttccatg caggccgatg cttctggcgc
     3841 tgttgtgacg agttcagata atcccatgaa ccatgtgtcc aacccacttg aaggaatcca
     3901 agtccacgcc ttggactttt ttaaccttcc aagtccccat atcaaaccca aacatatgct
     3961 ggtcctctac cagaaaatta aagaggaagc agataactac gatggagtgg tgatcacaca
     4021 cggaaccgat actttagagg aaacagccta tttccttgat accatggaag ttccccatat
     4081 gcctatcgtt ctaacaggag ccatgcgtag ctccaatgag ctcggtagtg atggtgttta
     4141 taattaccta agtgctttac gagtggccag cgatgacagg gctgctgaca aaggagtttt
     4201 ggtcgttatg aacgatgaaa tccacgctgc caagtatgtc accaaaacac atacgactaa
     4261 tgtcagcacc ttccagactc caacacatgg ccccctcggt ctcatcatga aacaggaaat
     4321 cctctacttc aaaacagctg aacctcgtgt tcgctttgac cttgatcaca tacaaggttt
     4381 agtccctatc atctcggctt atgctggtat gacagatgag ctgattgata tgctggattt
     4441 agaacacttg gacggtttga ttatccaagc cttcggagct ggtaatattc ccaaagaaac
     4501 ggctcaaaaa ttagaaagcc ttctgcaaaa aggaatccca gtcgctctgg tatcacgatg
     4561 ctttaacggt attgccgagc ctgtttatgc ctaccagggt gggggcgtac agttgcaaaa
     4621 agcaggcgtt ttctttgtta aagaactcaa cgcccaaaaa gctcgcttga aactcctcat
     4681 cgccctcaat gccggactaa caggacaggc tttgaaagac tatatggaag gctaatactc
     4741 ttcgaaaatc tctgcaaacc acgtcagcgt cgccttaccg tatgtatggt tactgacttc
     4801 gtcagtttca tctacaacct caaaaacatg ttttgagctg acttcgtcag tttcatccac
     4861 aacctcaaaa cagtgtgttg cgcagccttt aaccagccgc ctagtccgct ctatggtatt
     4921 cattaagtca acatctcttg tttaagagca caaaatcagg aaatcttctc gattccctga
     4981 ttttttctat ttacgttttc gtgttgagct acgttctgtc aaaccatgag gtaagagaac
     5041 ttcacgttct tccaactctt ccttatgcat aatcttggtc aacatacgca tactaatagc
     5101 accaaggtca taaagaggtt gggcaatcgt tgtcaagttt ggacgggtaa agcgtgagat
     5161 ttgtgaatca tcactagtaa taatttcaaa atcttctggc acagaaacac ctttatcagc
     5221 caaaccgttc aagactcctg ctgccaactc atcacctgtc acaactgctg cagttgcatt
     5281 tgatgaaatc aaacgctctg ctaaggcgta accatcatca tagctatatt tagattcaaa
     5341 taccaaaccc tcactataag tgattcctgc ttttttcaag gtttccttgt agccaactaa
     5401 acgaacctta ccattgatgt catccactag cggaccgcta acgaaagcaa tacgctcatt
     5461 ttctttagca aggtaactca ctgcatcaat tgttgcttgc ttatagtcaa tattgacact
     5521 tggcaactgg tgctcaacat cgacagttcc tgcgagaaca atcggagtac gtgaacgcga
     5581 aaattctgag cgaattttat ctgtcaagtg ataacccata tagataatgc catctacctg
     5641 ctttgaaaag agggtattga caacagaaac ttctttctcg ttatcttcat cgctattagc
     5701 taggacaata ttgtacttgt acatttctgc aatatcatca atccccttag ccaaactcga
     5761 aaaataacca ttggtaatat ttggaatcac gacaccgaca gtggttgtct ttttacttgc
     5821 aagaccacgc gcaactgcat ttggacgata atccaaacga tcaattacct ctagcacttt
     5881 tttacgggta ttctctttta catttttatt gccattgacc acacggctga ccgtcgccat
     5941 ggaaacacct gcttcacgag cgacatcata aatggttact gtatcatctg cattcattcc
     6001 ttttcctgtc ctttctatct cacatattct tttacaagta gaggtgctga ttgaagctct
     6061 atatctactt acaaaagtga agatgtgaaa atttcgtttt catatttcta cttattccat
     6121 tctatcacta attgtaaaca ctttcaagtg ttttttgaag attgattgaa aaaatttcat
     6181 agaaaaccta ggtttagctc cttgctacca ccttagacta aacaaaaagg aggaaactaa
     6241 gccctcctaa agttatagta aaatgaaata agaacaggat aaatcgatca ggacagtcaa
     6301 atcgatttct aacaatgttt tagaagtaga ggtgtactat tctagtttca atctactata
     6361 ggtattgttc cattcactac cgtcaatttt agcacatagt cttcatgaaa atattatatc
     6421 atcataacca accagattct tttgcgatat tagctgcctc tgttcgatta ccagcatcca
     6481 gttttgaaag aatattggtg acatagtttc gaatagttcc gtttgagagg taaagctgat
     6541 ctgcgatttc ttggttggat aagccccggg cgattccctt taagacagca atctcttgtt
     6601 ctgtcaacgg attgggacgg gtcatcacca cctccatcaa ttcaggcgaa tactccttgc
     6661 gtccttcgag gacggtgtgc aaggtttgca tgaggtctgc aatgcttcgt tcctttaata
     6721 cataagcatc cactccagcc ttgaccgcac gttcaaaata cccagcacgc ttgaaggtcg
     6781 tcaccacaac cacctttgtt tcaagccttt ctgatcgtat ccactccaag acttcaagac
     6841 ctgtcttaac aggcatttct acgtcaagga tggtgatatc tacagactcc ttttctaata
     6901 gttggattgc ttcttgccca ttcttggctt gaaggacaga ctccacatct ggttgaagcg
     6961 tgagcaattg acacatggca tctcgcaaca tactttgatc ttctgcgact aatactttca
     7021 tcttagtttc tctccttata aggtagtcga acttgcactt ccgttggatg tttctgactg
     7081 attacactta cttctcctga aaatggaaaa acacgatctc ggactgtatg gagctcatct
     7141 ccttttagaa aagcaaagcc gcagccatca tcacttactg ttaaaatgag ttccttctct
     7201 gtccgttcta attttaagta agctttagac gctttggcat gtttgatgat attggtcacc
     7261 aactcaagca aaatcataga agccattgac tccaattctt gagtaaggct agcagtatct
     7321 agttggttat ccgtttccac ctcaattcca gcaatttcta acatcttttt cacagtctct
     7381 agttcggatg tcaaagttct agacttaaga ttttccacaa tggttcgaac ttcacgcatt
     7441 gattctttgc taatttgctg tatttctctt aattcctttt ccacttgtgg ataagcctgc
     7501 atttgaaata gctgcaaggc tagatctgtc ttgacactaa gcatagcaaa ggtatgtccc
     7561 agactatcat gcaaatcctg accgatacga ttgcgctcat tttctgctag caatagattt
     7621 atctgggtat tttgcttgac ctgagcttct ttcaaatcct cgacaaggcg aattcgaacc
     7681 gagcccaaag tcatcaaatc gacaaaagta agaattacaa gcatatagac tagaaactca
     7741 acttcgattc tctgaaaaat caaaagtcgc cccacaacaa ggacttgagc aaggagaaat
     7801 gtccatacat gtaaagactt taaactacgt acgccaaaat gataaattaa gagattggaa
     7861 aggaaaaaga aaaaccagat ataattccca gcaacaaagg cggtattccc agctacatag
     7921 accagcatga gcccccagaa aagccaagat aggcgctggt tcttagttat taaaacaccc
     7981 aaatacgtca ctacaaatag aatatcaacc aataaatgcc aagcaggaag ctccccaatc
     8041 actacaggta ggatgggaaa aagcataaaa atcaaactgg cccaaaacat atagtgtatg
     8101 cttttcagtc tttcaagcat taagcattct ccttatgacc ttgaaggtaa atagtcaaac
     8161 caaataaaac tgctgaaaaa acaagtaaat aaactgtggc tgatagattg atgccaccct
     8221 cgtttaagaa ggtcttgagc aactccatca attgataggt cgggagacac ttaccaactg
     8281 cttgcatcca gtctggaaat aaagaaatgg gcatccagag tccgcctaaa acagccaatc
     8341 ctagataaag aagattgccc acaacagaca tcagctgact agttggtaag agagtcaagg
     8401 tcaagccaag tgctacaaaa gcaatactcc ccacaatcag caaaatcgca gctccaaccc
     8461 attttactag aggcatgtct acacctctta caaaatgtcc aactgtaaaa acaaccagaa
     8521 ttgagaccaa ataatcaacc agcatacttg ttatctttga tagataatat tctaccatat
     8581 ttacagggct atgacgcaat gttttctgcc agttgttcat cttatcggta tgtaaaacag
     8641 ctggaaatga gaggatagtt gtcgacatca tggaaaaggc agtcatggag atgaggtaat
     8701 cacgcataaa attcgcgagt tcacctggtg tgtcctgata gataccagaa aaaaataaat
     8761 agaaagccgt cggcatccct accgacaata gataatagat taattgtcgt ttggtcaata
     8821 aaaattctat cttactaagt gctatccatc gtttcatctt agttatctcc cttctgcgtc
     8881 tcttcaaaga ttgtattcaa caaactacga ttattaactt cgatttcttg catcctacat
     8941 cctgcttggg ctaatagttc ccagaaagca tctgcttctc gagtgactac ttgtaaagaa
     9001 tcttgcttta gggtccagtt ttcaaccaaa tttgactgct ctacgacttc cttgtaagct
     9061 ataggaagga taaagtgctt ttctatttcc tcgctacgca tagctagagg cgtcgtatct
     9121 cgaatcaact ctcccttatt taagagcaaa atgcggtctg cagtgtgttc cacttcctca
     9181 atataatggg acgaatagag aatggtgact ccttgcgctt ttaactcctg gacaatttcc
     9241 caaaaacgtt gacgagttga ggtatccatg gacgcagtag gctcatctaa aaaaacaatc
     9301 tttggtcgcc caattaaggt caagacgaaa gaaaagagac gcttttgacc gcctgataat
     9361 ttttctgcca actgttcttt ttgttgcttg acaaactgca atagttgatc aatttcctga
     9421 ttgctcaaag gatttggata gatacgttga aagaaagcaa tcaactcttt aacttttaat
     9481 ttctgaacga ttacattttc ttgagggagg taagccctag tatagtctaa cttagaactc
     9541 gtcactggta aaccttgaat agatacttga ccgcttgtga ccagtttatc tccaagcaaa
     9601 caatctaaga gtgttgtctt tccggcacca tttggaccaa ttaaggcaac acattcacca
     9661 tcgttgattt caaaggagat attccgcaaa atttccttgt cttttatttt cttactcaat
     9721 ttctcaactt taaccaccgt catgagactc tcctttcaac cactccattc ccataaggaa
     9781 aacgacgaaa atcataaatc caaaccccaa agcaccacga atgaattggc gaagcaaggc
     9841 ttgatcaaac caatcagtaa acatttccac taaccatacc aagagtgaca ggccgataaa
     9901 gaaatagatg atccctctct tcattcctca aactcctttt tcacatctct gactaatttc
     9961 aaaccttctc taacaagcca agacatgatt ccaaagccag caaagacctt ccaaggaaaa
    10021 tggtagaaat cctcatccaa tcccgaaaac atgagatagg tcataactcc tgctactact
    10081 aaacttactg cgataatcat tttatttctc atctcttctt cccccatttc atactacaat
    10141 tatagtcttt taaaatcaga ggagacagaa gcttctgtca ctagaaaata tgacaaatgt
    10201 cataaaaaat tctgttcaaa acaagcaaga tacactatac aataaaacac aatcagaaaa
    10261 atctaaggca acttcctcaa aagagatatc aaacccaatt cacaccataa tgtaaactaa
    10321 tacttattta aaatcaaaaa gagtaaaaat ttttatcaga caaacac
//
LOCUS       DAVLVZ010000062        10333 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000062 DAVLVZ010000000
VERSION     DAVLVZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.062"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..306
                     /locus_tag="VYN77_001635"
                     /pseudo
     CDS             1..306
                     /locus_tag="VYN77_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            404..616
                     /locus_tag="VYN77_001636"
                     /pseudo
     CDS             404..616
                     /locus_tag="VYN77_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            516..962
                     /locus_tag="VYN77_001637"
     CDS             516..962
                     /locus_tag="VYN77_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1559073.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1100..2404)
                     /gene="eno"
                     /locus_tag="VYN77_001638"
     CDS             complement(1100..2404)
                     /gene="eno"
                     /locus_tag="VYN77_001638"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1559074.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2568..3014
                     /locus_tag="VYN77_001639"
     CDS             2568..3014
                     /locus_tag="VYN77_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1559075.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3011..3814)
                     /locus_tag="VYN77_001640"
     CDS             complement(3011..3814)
                     /locus_tag="VYN77_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1559076.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPSGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3823..4468)
                     /gene="serB"
                     /locus_tag="VYN77_001641"
                     /pseudo
     CDS             complement(3823..4468)
                     /gene="serB"
                     /locus_tag="VYN77_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
     gene            complement(4527..5960)
                     /gene="glgA"
                     /locus_tag="VYN77_001642"
     CDS             complement(4527..5960)
                     /gene="glgA"
                     /locus_tag="VYN77_001642"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1559077.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSFGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDHILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5957..7096)
                     /gene="glgD"
                     /locus_tag="VYN77_001643"
     CDS             complement(5957..7096)
                     /gene="glgD"
                     /locus_tag="VYN77_001643"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1559078.1"
                     /translation="MKIDKYSAILGNTVDFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7086..8228)
                     /locus_tag="VYN77_001644"
     CDS             complement(7086..8228)
                     /locus_tag="VYN77_001644"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1559079.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8218..10146)
                     /gene="glgB"
                     /locus_tag="VYN77_001645"
     CDS             complement(8218..10146)
                     /gene="glgB"
                     /locus_tag="VYN77_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1559080.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPEKKWKDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTVPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3143 a         2274 c         1824 g         3092 t
ORIGIN      
        1 aatccagacg cttatttgac tgaaatagct tctgaatttg gctgtcatcc aactaccatc
       61 cactatgcac tcaaagctat gggctacact cgaaaaaaag aaccacacct actatgaaca
      121 agacccagaa aaagtagcct tatttcttaa gaattttaat agtttaaagc acctagcaca
      181 ggtctaacaa atggtgaatt aatcgctcca atgacttacg aagagatgat gacgagcgac
      241 ttttttgaag tatggtttca gaagtttctc ttaccaacat taaccacacc atcggttatt
      301 atagtgaaat gaaataagaa caggacaaat cgatcaggac agtaaaatcg aattctaaca
      361 atgttttaga agtagaggtg tactattcta gtttcaatct actatattaa tggacaatgc
      421 aagattccat agaatgggga agctagaact cttgtgtgaa gagtttgggc ataaactttt
      481 acctcttcct ccctactcgc ctgagtacaa tcttattgag aaaacatggg ctcatatcaa
      541 aaagcacctc aaaagggtat taccaagttg caataccttt tatgaggctc ttttgtcttg
      601 ttcttgtttc aattgactat ataaattgtc taagcgaaac aaccgataag aattggcaca
      661 aaagcgaccg tatttttgtt accaatacag gaaaaccagt tcatagttct atcttgagca
      721 agtctctcca gcgagcaaac gaacgcctta aaaaaccaat tcccaaacat ctgtcccctc
      781 acatcttcag acacaccact attagcatct tatcagaaaa taaaattcct ttaaaaacaa
      841 tcacggacag ggttggtcat tccgactctg aagtcactac ttccatctac actcacgtca
      901 caaagaacat gaaagatgaa gcaatcaatg tactggataa agttatgaaa aagatttttt
      961 aaaaagtttt gtcccttttt tgtcctctaa atacaaaaat agcccttcgg ataaaatccg
     1021 aggggctaga aacgttgtta aatcaacggc cgaacttttg aatttcatgg ttcgggataa
     1081 aatagttcac tgaactattt tattttttaa ggttgtagaa tgatttcaat ccacgatatt
     1141 cagctacttc accaagttgg tcttcgatac gaagcaattg gttgtattta gcgatacggt
     1201 ctgtacgtga aagtgaacca gtcttgattt gtcctgcgtt agttgcaact gcgatatcag
     1261 cgattgttga atcttcagtt tcacctgaac ggtgtgatac aacagcagtg taaccagctt
     1321 ctttagccat ttcgatagct tcaaaagttt cagtaagagt accgatttgg ttaactttga
     1381 taaggattga gttagcagca ccttcttgga taccacgtgc aaggtagtca gtgtttgtta
     1441 cgaagaagtc gtcaccaaca agttgtactt tcttaccaag acgttcagta agagctttcc
     1501 aaccatccca gtcgttttca tccataccat cttcaatagt gatgattggg tatttgttaa
     1561 ccaattcttc aaggtagtcg atttgttctg cagatgtacg aacagcagca ccttcacctt
     1621 caaatttagt gtagtcgtaa actttacgtt ctttatcgta gaattctgat gaagcacagt
     1681 caaatccgat aaatacgtct ttacctggta catatccagc agcttcaatc gcagcaagga
     1741 tagtttcaac accatcttca gttccttcga aacgaggagc gaatccacct tcgtcaccta
     1801 cggcagtttc caaaccacgt gatttaagga ttttcttaag agcgtggaag atttcagcac
     1861 cgtaacgaag ggcttcttta aatgttggcg caccaactgg caagatcatg aactcttgga
     1921 aagcgattgg agcgtcagag tgagaaccac cgttgatgat gttcatcatt ggagttggaa
     1981 gaactttagt gttgaatcca ccaaggtagc tgtaaagtgg gatttcaagg tagtcagcag
     2041 cagcacgagc tacagcgata gacacaccga ggattgcatt cgcacccaat ttacctttgt
     2101 taggagtacc gtcaagtgcg atcatagcac ggtcaatagc ttgttgatca cgtacatcgt
     2161 agccaatgat agcttcagca atgatgttgt ttacgttgtc aacagctttt tgtgtaccaa
     2221 gaccaccgta acgagatttg tcaccgtcgc gaagttcaac tgcttcgtgt tcaccagtag
     2281 aagctcctga tggaaccata ccacgtccga aagcacctga ttcagtgtaa acttctactt
     2341 caagtgttgg gttaccgcgt gagtctagga cttcgcgagc gtaaacatca gtaataattg
     2401 acatttttta ctctccttat gagttaaatt ttttacacct ctataatacc ttaaaacccc
     2461 tcctttttca agaaaaaacg ttatctttgt gcaacttttc cttaacttta taaagtaatc
     2521 gctttctttt gtctgtttta ttctaacttt tatgatatac tgttttcatg acagatttat
     2581 caaaacaatt acttgaaaaa gctcatggtg ggttaaaaat aaatccggat gagcaaagac
     2641 gctatcttgg tacttttgag gaaagagttc ttggatatgt agatattgac acagcaaata
     2701 gccctcagtt agaaaaaggc tttttattta ttttagaaaa ccttcaggaa aaagcagagc
     2761 cactatttgt gaagatttca ccaactatcg aatttgataa gcaagttttc tacttaaaag
     2821 aagcaaaaga aactgatagt caagccacca tagtatctga agagcatatt acttctcctt
     2881 ttggcctggt tattcatagc aatgcaccag ttcaagtaga agaaaaagac cttcgacttg
     2941 cttttccaaa actttgggaa gttaaaaagg aagaaccagc caaaacatcc ttatggaaga
     3001 aatggtttag ctaaatcttg cacatattta ataagtgccc aatattggca gccgtgcgct
     3061 ccagatagag actggcattt ttcaaactat cttctaaagg ttcacttttc tccaaaatag
     3121 aaaagacagc ttggatattt tcaaatggta ggggaggtaa atcttcagca agactaccgc
     3181 aaatagcaat aacaggaact ccaacagggg ttctttttgc aacacctata ggcgctttcc
     3241 cagcaaagct ttgactatca agtcttcctt ctccaacaac aaccaagtca gcatctgaaa
     3301 ctttcttatc aaagttgatt aagtccaagc aggtatcaat tccagacacg atacttgcct
     3361 gagcaaaggc acacaaacca gcagcaatcc ccagtcctcc atcattactg gccgtgccac
     3421 caacaccgat ataaatatct ttaatccctt tagaaatgag atggagaatc aactctccaa
     3481 taccacaagt ttggatttga agtggatttc gtttctctag cggaattttt ccaagaccaa
     3541 ccaagtcagc tacttcaaat agtgccagtt cccctttttg aaaatagcgc atggcttctt
     3601 tttgtccaga agggtctgtc acttggatcc atttttcttt taggtcaaga gaatgtcgga
     3661 tagcatctac agtaccttct cccccatcac caacagggca gaggagacat tctacatctg
     3721 ctatcgattg ttggaagcct ctttttattg cttcagctac ctgttgagct gtcaagcttt
     3781 ccttaaacga atccggtgca attacaatct tcatattttc cctcattcta aacagtcaat
     3841 caaaggaaga acttctaaaa aatccctctt gtcaacatga tgtggtattt cttttttgag
     3901 cacttctttg gcacaaaagg cgattcctaa ctccgccgac ttcaacatta atagattatt
     3961 aaccccatca ccgattgcca cccttctttc tttagaaagt tttagtttct ttctccattt
     4021 ttccagagtc tcttttttga cctggggact tataatttgt ccaactaatt ttcctgttaa
     4081 aagaccttct ttgacttcaa gctggttggc agtgaaatag gcaataccaa gggattttgc
     4141 taatctctcc aactattggt gtaaatccac cagacaccag accaactagg atgccattct
     4201 tttggagaat agagatgaac tctgggacat ttagcgatag atgaattgag ttgaagacgt
     4261 tatcaaagac caaaatagga agaccttcca acaaggacac tctttttctt aaactgcttt
     4321 caaagaccaa ctctcctcgc attgcccgac ttgtaatctg cgaaatttcc gcctcatgac
     4381 ctgcctctct ccctaaaaga tcaatcactt attctaggat taaggttcca tctacatcca
     4441 aaacacacaa gccttttact tgagacatca gttctcctct ctaaacagcc taaaaatcgt
     4501 atgaagtcat catacgattt tatctattaa ttaactaaac tatggtacaa gtcaaggtat
     4561 gacttgcagg ctgtatccca tgagaagtca ctctccatag cttgtttttg taggtttctc
     4621 caaatgtctg gatggtttct atacaagtcc aatgctgttt ggaaagtcca atttaaccaa
     4681 taaggagata gattgtcaaa gctaaagcca gtaccgcttc cttcgattgg attgaaagcg
     4741 cgaactgtat ctcgcaagcc tccaacttca tggaccaatg gcaaggttcc ataacgcata
     4801 gccatcattt gagacaagcc acacggttca aaacgacttg gcatgaggaa gaggtcacaa
     4861 gcagcgtaga tttcctgagc aagtttgaca tcaaaagtga tatttgttga tagcttgtct
     4921 gggtaaatct gagcaaacca tgagaaagct ccttcaaagg ctggatcgcc agttcccaaa
     4981 agaacaatct gaacatcttc ttgcaagata tggtgaagac tttcgaccac cacatcaaaa
     5041 cctttttgac gtgtcaaacg agaaacaatt cccaccagtg gaacgtctgc tctaacaggc
     5101 aagccaactc tttcttgcaa ttttgcctta tttttggctt tcccagacaa atcttcctga
     5161 ttgaaatgat agtctaaaag agcatccgtc tgaggattat aaagatcagc atcaatccca
     5221 ttcacgatac cagatacttt accagactcc attttaagaa tatgatccaa attacatcca
     5281 aactgactag tcataatttc atgagcatag ctaggtgaaa cggttgaaac acggttcgca
     5341 tagagaatac ctgccttcat ccagttcaga cagttgttcc atcgaagggt gccatcagcg
     5401 taacgttcaa agccaactcc aaacaaatca cccaacattc cttctgaaaa ttgtccttgg
     5461 aattctaaat tatgaatggt taaaactgtt tcaatgtcct cataggcttg aatccaacgg
     5521 tatttttcct tcaacaagaa aggaatcata gctgtatggt agtcatgaac atggagaaga
     5581 tcaggaataa agtcaatcct ttccatagcc tcaatggcag ccagttggaa aaaggcaaag
     5641 cgttctccgt catcaaaatc accgtaaaca tgaccacgga agaaataata ttgattgtca
     5701 ataaagtaga aggttacacc atttaatact gttttcttaa ttccacaata ctgtctgcgc
     5761 caaccaaagc tcacctcaaa atgaagcaca tcttcaatct gatttccaaa tttagcctct
     5821 accatatcat agtagggtaa aatcactgca acttcgtgcc cagcttttac cagtgatttt
     5881 ggaagagcgc caatgacgtc tcccaaacca cctgtttttg aaaagggtgc accctctgct
     5941 gctacaaata aaattttcat gaatgaatat cctctgttac tttagcacct ttcttaacca
     6001 caactggatg ttctgcagtt cctcgaatca caacaccagg ctcaacttca acccctttgt
     6061 ccaagatagc atattcgacc tgagcccctt ctccaataac aacacgaggg aagagcaggc
     6121 tatctttaac caagctatcc ttatggacat gaatattacg tgatagaaca gaattagcta
     6181 cttgaccttc aataatacta ccagaggcaa actgagaagt gcttacctta gatgtattag
     6241 catagtaagt tggctcttcg tttttgacct ttgtataaat cttttggttt ggtgagaaaa
     6301 gagaatagaa tttttgtgat tcaagcatat cgatattcgc ttgataataa gatttaacag
     6361 agtgaatatt ggctagatag cccgtgtact cgtaggcaaa agctccctct tttacagcca
     6421 aatcccgtaa aacatagcgc aatttctctg gatgttcttt tttagcttct tcttccaagt
     6481 gttcaatcaa ccaaggtgta tcaacgacaa agatatctgt agacatattg aacgtttcag
     6541 ctgttgactt gctatcaaag agtttatgag aaagaacatg gtctgtttca tctacatcca
     6601 agattgcatt tacttctgaa atatctttct tagctagttt tttataaact acagtgatag
     6661 gctcttttgt tgtactatgt aggtggaaaa cttggttcaa atcaatgtta ataagaacat
     6721 cgcagttgag ggcaaccgtt tggtttgagc cagaacgttt caaataagta agaagctgtt
     6781 ggtagtattc ttttccaact gtactacttt ctacacgggt attgtaaatt cctagatagt
     6841 aatggctaag aagggttgat aagccccact cgcgtcctga acgaatatgg tcaaatactg
     6901 agctgatatt atcctgctgg aaaataccaa agacactacg aacacctgca ttagcaaggc
     6961 ttgaaagtgg gaagtcaatc aaacgatatt tcccaccaaa tggcaaactt gctactggac
     7021 ggtggtccgt caatgtcgac atattgtgaa aatcaactgt atttcctaaa atggcagaat
     7081 atttatcaat cttcatctgt tgctaccccc actacttcat tatatcctac aacttgtact
     7141 tcatctgttc catcaatttc gacaccgtca gaaataatcg caccttcacc aataatggca
     7201 cgtttaatct tagctccttg accaatgata gctccactca tgataactga atcaaggact
     7261 tccgctcctt cgcgaacttg cgcgcctgtt gaaaggatag aatgtttaac agttccatca
     7321 acgaaacatc cgtctacaac taatgagtct tccacatgag catttgcccc gaggaagttt
     7381 ggtggtgaaa tcaagtttct tgagtaaatc ttccattgac ggttacgact atccaaggca
     7441 ttttctggag aaatatactc catgttcgct tcccaaagtg actcaatagt accaacatct
     7501 ttccaataac cactaaattc gtaagcataa acactttcac ctgactcaag gtaatttggg
     7561 atgacatttt taccaaagtc tgacatgcca accttgctct tttcagcagc gactaacata
     7621 ttacgaaggc gttgccaatc aaaaatgtag attcccatag aagcttttgt agatttaggt
     7681 tgagctggtt tttcttcaaa ttcaacaata cgattgttag catctgtgtt catgatacca
     7741 aaacggcttg cttctttaag agggacgtct aaaactgcta ctgtcaagct ggcattatta
     7801 tccttatgag actggagcat atcatcatag tccattttgt agatgtggtc cccagacaaa
     7861 atcaagacat actcaggatt gacactgtcg atatagtcga tattttggta aatagcgtga
     7921 ctagtcccct caaaccaacg atttccttca cttgcagaat aaggttgaag aatagagaca
     7981 cctgaattaa taccgtctag tccccagctt gaaccattcc caatatggtt gttgagagca
     8041 agtggttgat actgtgtaac aaccccaaca ttatgaatcc ctgagttggc acagtttgat
     8101 agggcaaagt caatgatacg gtagcgccca ccaaattgca cagctggttt tgcgatgctt
     8161 tgagtgagtt taccgagacg agttccttgc ccaccagcaa gaatcaaagc taacatttca
     8221 tttttcattt tctactcctt tttggttttt atttgtgacg gttttagtag atttcaagcg
     8281 acgtttgatt ttccatacac ttgctcccat agccggtacg gtaaaggtta aggtctgctc
     8341 ataatctttc catagtcctt cttgcgtttg aacagtttga ttatgttctt tccaaacgcc
     8401 tccccactct tccaactcag tattccatac ttcttcgtaa attcctgcaa cgggtagtcc
     8461 gattgtaaaa tctttccgct caacaggtac catgttaaag atacagacta acatttctcc
     8521 ctttttaccc ttacgaataa aggaaagaac actctggtct cgattatccg catcaatgat
     8581 ttcaatacca tcatagctgg tatcgatttc ccacagacag cgatgatctt tgtaaaactg
     8641 gtttagctga gaagtgaaat acttcatctt agcattcatt gggtcttcta ggttagacca
     8701 ttccaactgt tcttcagatt tccattctag gaattgaccg tattcgctac ccatgaagag
     8761 caatttctta ccagggtgac aaatttggta cgtatagaga ttgcgcaagc ctgcgaattg
     8821 attgtaacga tctccccaca tcttatgcat catactcttc ttgccatgaa ccacttcatc
     8881 gtgcgagaat ggcaagagat aattctcctt gaaaacatac ataaagctga aagtcaccag
     8941 gttaaagtca tatttacgat aaatcggatc ttcttcgtag aaacggagga tatcattcat
     9001 ccagcccatg ttccatttgt agtcaaatcc tagaccacca atctctttca ttcccgtaat
     9061 cttggttgca gacgaacttt cttctgcaat catcatcaca tctggatatt ctaacttaat
     9121 aacttcattc aagcgttgaa ggaaataata accttcatag ttgagatttc cgccatcttt
     9181 attaggtgtc catggagcat catcatagtc caaatagagc atgttgctaa cagcatccac
     9241 acgaatacca tccaaatgat agacatcaat ccaatgctta atgcaagaaa ttaagaagga
     9301 ctggacttca ttttttccaa ggtcaaaatt aagggcaccc caaccatggt tatgagcctt
     9361 attatggtct tggtattcaa aagtcggtgt cccatcataa taggctaagg catcatcatt
     9421 gatggtaaag tgacctggta cccagtccac aataacccca atattatggg tatgacactc
     9481 ctcgacaaaa tcttgaaact cctctggtct accataggca tgctctaaag cgaagtaacc
     9541 cataagctgg tacccccaac tcaaacctag tgggtgggac atcaagggca taaactcaat
     9601 atgagtatag ttcatctcaa ctaggtaagg aatgagttca tctttcaatt gggcaaaact
     9661 ataaggactg ccatcaggat ttctcttcca agagccagca tgaacctcgt aaatattgac
     9721 aggacgttca gcaaagcccc aacgttttct tcgtgccagc caaagtccat ccttccattt
     9781 cttctcagga agctctgtta cgattgcccc tgttcctgga cgagcctcat acctgacagc
     9841 aaaagggtca atcttcatca gttgatgacc attttgacgt gtgacatgat atttgtaaat
     9901 atgcccttct tgagccatat tggtaaagac ttcccagacc ccaaaatcat ttcttaccat
     9961 tggaatctga ttttcaatcc agttggtaaa atcaccaacc aagtgaacag cctgagcatt
    10021 aggtgcccaa acacggaagg tatagccatg ctctccattt agttcttccc tatgtgctcc
    10081 tagataatgt tggagataaa aattttcacc cgtcataaag gtttttaatg cttctctatt
    10141 atccatatac tccccttctc ctgtaagcgt tttctatgtt tttattatac taccttttta
    10201 gagaagattc aagtaaatta ctatacttct ttaattattt tgaaaatcta caacaagttc
    10261 acttactcgt tcaattgtaa atcaatattt tttcaaaaaa ttgcgaaaac gcctttcttt
    10321 ttctactata gtg
//
LOCUS       DAVLVZ010000063        10323 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000063 DAVLVZ010000000
VERSION     DAVLVZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.063"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            233..709
                     /locus_tag="VYN77_001646"
     CDS             233..709
                     /locus_tag="VYN77_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1559081.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEALYAYIAEATA"
     gene            747..1049
                     /locus_tag="VYN77_001647"
     CDS             747..1049
                     /locus_tag="VYN77_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1559082.1"
                     /translation="MIKILAACGAGVNSSYQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIRLPAKQNMV"
     gene            1057..1860
                     /locus_tag="VYN77_001648"
                     /pseudo
     CDS             1057..1860
                     /locus_tag="VYN77_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1909..2373
                     /locus_tag="VYN77_001649"
     CDS             1909..2373
                     /locus_tag="VYN77_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1559083.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2530..2955
                     /gene="lacA"
                     /locus_tag="VYN77_001650"
     CDS             2530..2955
                     /gene="lacA"
                     /locus_tag="VYN77_001650"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1559084.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2986..3501
                     /gene="lacB"
                     /locus_tag="VYN77_001651"
     CDS             2986..3501
                     /gene="lacB"
                     /locus_tag="VYN77_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1559085.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3512..4441
                     /locus_tag="VYN77_001652"
     CDS             3512..4441
                     /locus_tag="VYN77_001652"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1559086.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            4443..5423
                     /gene="lacD"
                     /locus_tag="VYN77_001653"
     CDS             4443..5423
                     /gene="lacD"
                     /locus_tag="VYN77_001653"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1559087.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5656..6492
                     /locus_tag="VYN77_001654"
     CDS             5656..6492
                     /locus_tag="VYN77_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1559088.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6528..6845
                     /locus_tag="VYN77_001655"
     CDS             6528..6845
                     /locus_tag="VYN77_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1559089.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6845..8536
                     /locus_tag="VYN77_001656"
     CDS             6845..8536
                     /locus_tag="VYN77_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1559090.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKVG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            8751..10157
                     /gene="lacG"
                     /locus_tag="VYN77_001657"
     CDS             8751..10157
                     /gene="lacG"
                     /locus_tag="VYN77_001657"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1559091.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGFGYKDEFVNNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            10200..>10323
                     /locus_tag="VYN77_001658"
     CDS             10200..>10323
                     /locus_tag="VYN77_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV1559092.1"
                     /translation="MFKDFIQSIYEKVYIINFEKYSQIPCLTSEELKSLGKWYVS"
BASE COUNT         3301 a         1843 c         2117 g         3062 t
ORIGIN      
        1 atgttttgag caacctgcgg ctagcttctt agcttactct ttgattttca ttgagtatta
       61 gaacagaaaa aatgcttgga gtatttgttt gtgtgtttac ttttatataa caaactataa
      121 acacaacaaa aatataaaaa aagagacaaa aaaagaacag aaagtaatta acaacaattt
      181 aatatattgt tatacttata gcataaagtt aatggaaaga agggaaaacc ttatggtatt
      241 agattatttc tttgacaaaa accttgtgtt ttgcttagaa gcggataatc aagaacaact
      301 ctttgatcag gtagcaagct tgttggaaga acgagaaatt gtcactccaa cttatcgtga
      361 agccttgatc acgcgtgaaa agtcatttcc aactggttta gatatggaat ttctaggaaa
      421 ggacttgcca aatgtagcga ttcctcacac agatattgtt cataatttgg ctgaaaaagt
      481 tgtggtcgtt cgattagaga aaccggtgac tttccataat atgattgcgc cagataagga
      541 agtagaagta tccttgctct tctttatcat taataattca agttcaagtc aaacgaacat
      601 tcttgctcag ttaatggact tcttcacagg aaatggtcat cttgaagacc tatcaaaaat
      661 ttctgaacca gaagcccttt atgcttacat tgctgaagca accgcttaat cttgtctatt
      721 aaaaaataaa atcggaggaa atccaaatga ttaaaattct tgctgcctgc ggtgcaggtg
      781 ttaactcaag ttaccaaatt aaaagtgctc tagaagaaga actttcaaac cgtggttatg
      841 atgttcactg tgatgcagtc atggtgaaag atgtaaacga agaccttatg aaaggttacg
      901 atatctttac accaatcgct gcaacagatc ttggttttga accaggtatc ccagttatcg
      961 aagctgggcc aatcttattc cgtataccag caatgagcgc tccagtattt gacaatatta
     1021 gactccctgc gaaacaaaat atggtatagt agttctatga atgatgaagc aagtaaacaa
     1081 ctaactgatg cacgatttaa gcgtcttgtt ggtgttcagc gtaccacttt tgaagagatg
     1141 ttagctgtat taaaaacagc ttatcaactt aaacacgcaa aaggtggacg aaaacctaaa
     1201 ttaagcctag aagaccttct tatggccact cttcaatatg tgcgagaata tcgaacttat
     1261 gaagaaattg cggctgattt tggtgttcac gaaagcaact taatccgtcg gagccaatgg
     1321 gttgaagtaa ctcttgttca aagtggtttt acgatttcaa gaactcctct cagttctgag
     1381 gacacggtaa tgattgatgc gacggaagta caaatcaatc gccctaaaaa aagaattagc
     1441 gaattattct ggtaaaaaaa atgccacgct atgaaggctc aagcgattgt cacaagtcaa
     1501 gggagaattg tttctttgga tatcgctgtg aactattgtc atgatatgaa gttgttcaaa
     1561 atgagtcgca gaaatatcgg acaagctggt aaaatcttgg ctgacagtgg ttatcaaggg
     1621 ctcatgaaga tatatcctca agcacaaact ccacgtaaat ccagcaaact caagccacta
     1681 acagttgaag ataaagccta taaccatgcg ctatccaagg agagaagcaa ggtcgagaac
     1741 atctttgcca aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa tcatcgtaaa
     1801 cgcttcggat tacgaatgaa tttgattgct ggtattatca atcataaact agaattctag
     1861 ttttgcagga agtctaataa agaaactagc aaggtcaggc cttactttat tggtggacat
     1921 ccaggattta attgtcttct acttgatgat gaagtctatg aagattatta tctagagttt
     1981 gagaaagaag agatttgctc tgttccacgt cctttcccag aaacaggtat gttggatttc
     2041 caagatagaa gtccatggct agagggacaa aaagaaatag atcttagtta tgatcttttc
     2101 agcacagatg cagtgacttt ggatgaattg caatctcgaa caattgccct tcgttctctt
     2161 aaacatgata agggattgaa agtgcatttt gcagaatttc caaacctcat catctggtca
     2221 actttgaaca agggaccttt cattaccttt gaaccatggt ctggcttgtc aacattcctt
     2281 gaagaaggag atcatttaga agataagaag aatgtttgtc tcttagaagc caatcaggtt
     2341 gaagaattag ggtttgagat agaagtttta taaaaaagta aaaaataaat aacaaaaaca
     2401 aacacaaact gttgacttct tcaaacaata gtagtatatt atgtttgtga ggactaattg
     2461 actgtttttt aaagacagcc aatacactgc tggaatccag catagaaaaa ataaaaagga
     2521 gtatacaata tgtctattgt tatcggtgca gatgctgcag gtttgagatt gaaagaagtt
     2581 gtgaaagact tcttagaaaa agaaaacttc caccttgtgg atgttacagc tgaaggtcaa
     2641 gactttgttg atgtgactct cgctgttgct gcagaagtaa acaaagaaga acaaaacctt
     2701 ggtatcgtga ttgatgctta tggagctggt ccatttatgg ttgcaactaa gatcaaagga
     2761 atggttgctg cagaagtatc tgacgaacgt tcagcttata tgactcgtgg ccacaacaac
     2821 tcacgtatga tcactatggg ggcacaactt gttggtgatg aattggctaa aaacatcgct
     2881 aaaggatttg ttaatggtaa atacgacggt ggtcgtcacc aaattagggt cgacatgttg
     2941 aacaaaatgg gctaattaga ttatagtaag aaaggtaagt taaaaatgag aattgcaatt
     3001 ggatgtgacc acatcgtaac agatgaaaaa atggcggttt cagaattttt gaaatcaaaa
     3061 ggatatgaag tcattgactt tggtacctat gaccatacac gtactcacta cccaatcttt
     3121 ggtaaaaaag taggggaagc agtaactagt ggtcaagctg atctcggggt atgtatctgt
     3181 ggtactggtg ttggtatcaa caacgctgta aataaagttc caggtattcg ttctgccttg
     3241 gttcgtgata tgacaacagc cctttatgct aaagaacaat tgaacgccaa cgttatcggt
     3301 tttggtggta agattactgg tgaattgctt atgtgtgata tcatcgaagc tttcattcat
     3361 gctgaataca aaccatctga agaaaacaaa aaattgattg cgaaaattga acaccttgaa
     3421 agtcacaatg ctcaacaaac agacgcaaac ttcttcactg aattccttga aaaatgggat
     3481 cgcggagaat accacgacta agaggtaacc tatgatttta acagttacaa tgaacccatc
     3541 cattgatatt tcctatccct tggatgagtt gaagattgat actgtcaatc gtgtggtgga
     3601 tgtaaccaaa acggctggtg gtaagggact caatgttacc cgagtacttt cagaatttgg
     3661 cgattctgtt cttgctactg gtttagtggg tggcaaactt ggtgagtttt tggttgaaca
     3721 tatcgataat caagtaaaga aagatttctt ctcaattcag ggagaaactc gtaactgtat
     3781 cgctattctc cacggagaca accaaacaga agttcttgaa aaaggtcctg aagtattgga
     3841 acaggaaggt caagactttt tggaacattt caaaaaactc ttggagtcag ttgaagtagt
     3901 ggccatctca ggtagtctgc cagctggcct tccagttgat tactatgcga gcttggtaga
     3961 acttgctaat caagctggca agcctgtagt cttggactgc tcaggtgcag cacttcaggc
     4021 tgttcttgaa tcaccccata aaccaacagt catcaaacca aataatgaag aattgtctca
     4081 gcttcttgga agagaagttt ctgaggattt ggatgaatta aaagaagtac ttcaagaacc
     4141 tttgtttgca gggattgaat ggattatcgt ttcacttggt gccaacggta cttttgccaa
     4201 acatggtgac actttctaca aggtagatat tcctagaatt caggtggtaa atcctgttgg
     4261 atctggagac tctactgtgg caggaatttc ttcaggactt cttcacaaag aatcggatgc
     4321 agaattactc atcaaggcaa atgtccttgg tatgctcaat gctcaagaaa aaatgactgg
     4381 tcatgtcaac atggccaact atcaagttct atatgatcaa ttaatagtaa aagaggtata
     4441 aaatggcttt aacagaacaa aaacgtgtac gcttagaaaa actttctgat gaaaatggta
     4501 tcatctcagc tcttgcattt gaccaacgtg gtgctttgaa acgcctcatg gctcaacacc
     4561 aaacagaaga accaactgtg gcccaaatgg aagaacttaa agtcttggta gcagatgaat
     4621 tgactaaata cgcttcatct atgcttcttg accctgagta tggacttcca gcaactaaag
     4681 ctcttgatga aaaagctggt cttctccttg cttatgaaaa aacaggttat gacacaacaa
     4741 gcacaaaacg cttgccagac tgcttggatg tctggtctgc aaaacgtatt aaagaagaag
     4801 gtgcagatgc agttaaattc ttgctttact atgatgtaga tagctcagac gaactcaatc
     4861 aagaaaaaca agcctacatc gaacgcatcg gttctgagtg tgtggctgaa gatatcccat
     4921 tcttccttga aattcttgct tacgatgaaa aaattgcgga tgcaggttct gtagaatacg
     4981 caaaagtaaa accacacaaa gttatcggcg ctatgaaagt cttttcagac ccacgcttta
     5041 acattgatgt cttgaaagtt gaagttcctg ttaacattaa atatgttgaa ggcttcgctg
     5101 aaggtgaagt agtttataca cgtgaagaag cagcagcctt cttcaaagcg caagatgaag
     5161 caacgaactt gccatacatc tacttgagtg ctggtgtatc agctaaactc ttccaagata
     5221 ctcttgtatt tgctcatgaa tcaggtgcaa actttaacgg agttctttgt ggccgtgcaa
     5281 catgggctgg atcagttgaa gcatacatca aagatggtga aacagcagct cgcgaatggc
     5341 ttcgcacaac tggatttgaa aacattgatg agctcaacaa agttcttcaa acaacagcga
     5401 cttcatggaa agaacgtgtg taagaaagtc ctcctagttt aggaacatga atctaaaaaa
     5461 tttaaaaaaa gttgtatgta aaggtttaca aaataactta cttgtgctat acttaaatca
     5521 caagttaata caaggtgagt gttactaagt aatattaggc atgatcacag gtgaattaga
     5581 aatcagctga ttttctagtt catttgtggt cattttttgt actcatatac ctttaagata
     5641 taaaaggagg ttgacatgta tcgaattcta aatccaatga atcacaatgt ctcgcttgtc
     5701 agaaatgata agggagaaga ggtgattgta attggtaagg ggattgcatt cggaaagaag
     5761 aagggggatt tgattgctga aaatcatgtt gagaaaatct ttcggatgaa gaccgaagag
     5821 tccagagaaa actttatggc tcttctcaaa gatgttccgc ttgattttat cacagtgacc
     5881 tatgaaatca ttgataagct atcaaagaaa tatcattatc cgattcaaga gtatctctat
     5941 gtaaccttga cagatcatat ttactgttct tatcaagctc taactcaagg aaggtacaag
     6001 gatagtaatc tgccagatat ttctgctaag tatcctgtcg cttttcaaat cgcaaatgaa
     6061 gcttttgaaa tttaccgtca gaagctagca gatcattttc ctgaggacga aattattcgg
     6121 attgcttatc atttcattaa tgctgaaggt gaaaatgaag tggaacttgt ggagtcgatt
     6181 gataagagga aagaaattct caggaatgtt gaagaagttt taacggacta tgcaattcaa
     6241 cgaactaaaa agaataacca tttctatgat cgctttatga tccatttgaa ttatttcttg
     6301 gattatttag acagatctag agatgataac caatcacttc tggatatgga agatcatatt
     6361 aaacaatcct atccaaaagc cttcgagatt ggttccaaga tctatgatgt gattacgcaa
     6421 catacgggtc ttgatttgta taaaagtgaa cgagtttatc tagttctaca tatccaacgt
     6481 ttattgtcat aaaaatttat ttaaaactat ataaggagaa ttctatcatg aatagagaag
     6541 aagtaacatt gttaggtttt gaaatcgtag cctatgctgg cgatgctcgt tcaaaactat
     6601 tggaagcctt gaaggctgct gaagctggtg attttgaaaa agcggacgct ctggtagagg
     6661 aagctggtag ctgtattgca gaggctcacc acgcgcaaac aagtctattg actaaggaag
     6721 cttcaggtga ggacttggct tatagtgtaa ccatgatgca tggccaagac cacttaatga
     6781 caactatctt gttaaaagat ttgatgcatc atttaattga actctacaag agaggagtta
     6841 aataatgaat aaactaattg catttatcga gaaaggaaag cctttctttg aaaaactatc
     6901 tcgtaatatc tatcttcgtg ctattcgtga tggtttcatt gcaggtatgc ctgttattct
     6961 cttctcaagt atctttatct tgattgcctt tgtaccaaac tcatggggct ttaaatggtc
     7021 tgatgaagtt gtagcctttc tgatgaaacc ttatagctat tctatgggta ttctggctct
     7081 cttggtagct ggtacaacag ctaagtcatt gactgactca gtaaaccgta gcatggaaaa
     7141 aaccaatcaa atcaactata tgtcaacatt gttggcagca attgttggtt tgttgatgtt
     7201 ggcagctgat cctatcgaaa gtggtctagc tactggattc ttggggacaa aaggtttgct
     7261 ttcagccttc cttgctgcct tcgttactgt agccatctat aaggtttgtg ttaagaacaa
     7321 cgtcactatt cgtatgcctg acgaagttcc accaaatatc tcacaagtct ttaaagatgt
     7381 gattccattc actctatctg ttgtttctct ttatgctctt gacttattag cacgtcattt
     7441 tgttggttct agtgtggcag aatcaatcgg taaattcttc gcaccactct tctcagcagc
     7501 agacggatac cttggtatta ccattatctt tggtgccttt gccttcttct ggtttgttgg
     7561 gattcatggt ccatctatcg ttgaaccagc tatcgcagct attacctatg ccaatgccga
     7621 agtcaacttg aaccttctcc aacaaggtat gcacgctgac aagattctta cttctggtac
     7681 acaaatgttt atcgttacca tgggtggtac tggtgcgaca ctggttgttc cattcatgtt
     7741 catgtggtta acaaaatcaa aacgtaaccg tgcaatcgga cgtgcttcag tagttcctac
     7801 cttcttcggt gtaaatgagc caatcttgtt tggtgcacct cttgttttga atccaatctt
     7861 cttcattcca tttatctttg ctccaattgc aaacgtatgg attttcaaat tctttattga
     7921 aactcttgga atgaactcat tcactgctaa tctaccatgg acaactccag ctccactagg
     7981 tctagttctt ggaactaact tccaagtgct atcattcatt cttgctgccc ttctaatcgt
     8041 ggttgacgtt gtcatttact atccattcct taaggtctat gatgaacaaa ttcttgaaga
     8101 agaacgttca ggtaagtcta atgatgaatt gaaagaaaaa gttgctgcaa acttcaacac
     8161 tgcaaaagcg gatgctattc ttgaaaaagt aggtgtagaa gcagcacaaa ataccatcac
     8221 taaagaaaca aatgtcctcg ttctctgtgc aggtggagga acaagtggtc tccttgcaaa
     8281 tgctctaaac aaggcagcag cagaatacaa tgtccctgtg aaagcagcag caggtggcta
     8341 tggtgctcac cgtgaaatgt tgccagagtt tgatttggtt atccttgctc ctcaagttgc
     8401 ttcaaacttt gaagatatga aggcagaaac agataagctc ggtattaaac ttgctaagac
     8461 agaaggcgct caatacatca aattaactcg tgatggaaaa ggtgccctag cattcgtaca
     8521 agagcaattc gattaaggct aggaactgtg aaacagtctc ccatcgttac ggaaatcgct
     8581 atggcgaatt tcctaatcgc cttattaatt cgtcggtaaa aagatatcgt ttttacctcc
     8641 tcatgtcaca attcggtgac ttggtacaag aagtgagatg gagaaggatg gctcactgac
     8701 tcctctcctc tcacttttac tttatttaaa tcaagaaata ggtgaaaaaa atgacaaaaa
     8761 cacttccaaa agactttatt tttggtggcg caacagctgc ctatcaagca gaaggtgcta
     8821 cacatactga tggtaaagga ccagtagcgt gggataaata tcttgaagat aactactggt
     8881 acactgcaga accagccagt gatttttaca atcgatatcc agttgacctc aagctagcag
     8941 aagagtatgg tgtcaatggc attcgaattt ctattgcctg gtcacgtatt ttcccgactg
     9001 gttatggtca agtaaatgct aaaggtgttg agttttatca taatttattt gcagagtgtc
     9061 acaaacgtca tgttgagccc tttgtaactc ttcatcactt tgacacgcca gaagctctcc
     9121 actcaaatgg agacttctta aaccgtgaaa atatcgaaca ttttgtagac tacgctgcct
     9181 tctgttttga agaatttcca gaagtaaact attggacaac ctttaatgaa attggaccaa
     9241 tcggtgatgg tcaatatttg gttgggaaat tccctccagg tatccagtac gaccttgcca
     9301 aagtctttca atcacaccac aatatgatgg tgtctcatgc atgcgcggta aaattgtaca
     9361 aagagaaagg ctataaaggg gaaattggtg ttgttcacgc cctgcctact aaatatcctc
     9421 tagatcctga aaatccagca gatgttcgtg cagcagagtt ggaagatatc atccacaata
     9481 aattcatctt agacgcaact tatctaggtc gctattcagc tgaaaccatg gaaggtgtca
     9541 accatatctt atcagtcaat ggtggtagtt tggatcttcg tgaagaagat tttacagcat
     9601 tagaagctgc aaaagacttg aatgatttcc taggaatcaa ctactatatg agtgactgga
     9661 tggaagcctt tgatggagaa actgaaatta tccataatgg taaaggtgaa aaaggaagct
     9721 ctaagtatca aatcaaaggt attggtcgtc gtgtagctcc tgactatgta tcacgcacgg
     9781 attgggattg gattatctac cctcaaggtt tgtatgacca aatcatgcgt gtgaagaaag
     9841 attatcctaa ctacaagaaa atttatatca ctgaaaatgg tttcggctat aaagatgagt
     9901 tcgttaataa cactgtttac gatgatggtc gtattgatta cgtgaaacaa cacttggaga
     9961 tcttgtctga tgcgattgca gatggagcta atgtaaaagg ttacttcatt tggtcattaa
    10021 tggatgtctt ctcatggtca aacggttatg agaaacgtta tggtctcttc tatgtagatt
    10081 ttgaaactca agaacgttat cctaagaaat cagctcactg gtacaagaaa gtagcggaaa
    10141 ctcagattat agactagtag aattagtcat tagatataga attttagtga gtcaaaaaga
    10201 tgttcaaaga ttttatccaa tctatttatg aaaaagttta tattataaat ttcgaaaaat
    10261 actctcaaat accgtgtttg acgagtgaag aattgaaaag tcttggaaaa tggtatgtct
    10321 cga
//
LOCUS       DAVLVZ010000064        10194 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000064 DAVLVZ010000000
VERSION     DAVLVZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.064"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            4..77
                     /locus_tag="VYN77_001659"
     tRNA            4..77
                     /locus_tag="VYN77_001659"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Asn,seq:gtt)
     gene            complement(171..968)
                     /locus_tag="VYN77_001660"
                     /pseudo
     CDS             complement(171..968)
                     /locus_tag="VYN77_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1233..1469
                     /locus_tag="VYN77_001661"
     CDS             1233..1469
                     /locus_tag="VYN77_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV1559093.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            1700..2986
                     /locus_tag="VYN77_001662"
     CDS             1700..2986
                     /locus_tag="VYN77_001662"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1559094.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            3186..3653
                     /locus_tag="VYN77_001663"
     CDS             3186..3653
                     /locus_tag="VYN77_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1559095.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            3686..3779
                     /gene="ffs"
                     /locus_tag="VYN77_001664"
     ncRNA           3686..3779
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYN77_001664"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3839..4282
                     /locus_tag="VYN77_001665"
     CDS             3839..4282
                     /locus_tag="VYN77_001665"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1559096.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            4284..4799
                     /locus_tag="VYN77_001666"
     CDS             4284..4799
                     /locus_tag="VYN77_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1559097.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            4813..6174
                     /gene="radA"
                     /locus_tag="VYN77_001667"
     CDS             4813..6174
                     /gene="radA"
                     /locus_tag="VYN77_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1559098.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            6247..6744
                     /locus_tag="VYN77_001668"
     CDS             6247..6744
                     /locus_tag="VYN77_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1559099.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            6769..7552
                     /locus_tag="VYN77_001669"
                     /pseudo
     CDS             6769..7552
                     /locus_tag="VYN77_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            7697..8665
                     /locus_tag="VYN77_001670"
     CDS             7697..8665
                     /locus_tag="VYN77_001670"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1559100.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(8799..9080)
                     /locus_tag="VYN77_001671"
                     /pseudo
     CDS             complement(8799..9080)
                     /locus_tag="VYN77_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9207..9440)
                     /locus_tag="VYN77_001672"
     CDS             complement(9207..9440)
                     /locus_tag="VYN77_001672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559101.1"
                     /translation="MFNQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(9433..9549)
                     /locus_tag="VYN77_001673"
     CDS             complement(9433..9549)
                     /locus_tag="VYN77_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1559102.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(9764..10117)
                     /locus_tag="VYN77_001674"
     CDS             complement(9764..10117)
                     /locus_tag="VYN77_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1559103.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
BASE COUNT         2837 a         1685 c         2402 g         3270 t
ORIGIN      
        1 taatccgcca tagctcagtt ggtagtagcg catgactgtt aatcatgatg tcgtaggttc
       61 gagtcctact ggcggagtaa tttagttaag aggtttagac ctctttttta ttatgtaata
      121 ataatctatt tcgtgttata gtaaaatgaa ccaaaaatag tggctctttg tcaactgtag
      181 tgggttgaag aaagcgaaga tctagaaagg acaaatttcg tcctttcttt tttgaagttt
      241 tcaaagttcc taaaaccaaa ggcattgtac ttgataagtt tgatgagatt attggtggct
      301 tccagtttgg cgttggaata aggtaattga agggcgttga cgattttctc tttatctttg
      361 aggaaggttt taaacagagt ctgaaaaaga ggtggaaaag caagagctga tagagattat
      421 agtggtgttt caagtcttcg gaatagctca aaagtttatc tagaatttct ttattagtca
      481 agtgcatacg aaaagtaggg cgataaaatc gtttatcact cagtttctga ctatcctgtt
      541 gaatgagctt tcagtatcgc ttgatagcct tgtattcatg ggatttcgga tgatggcttg
      601 tgttctgctc tcaagaacag ttatgatatt gagcttatca aagtcttgag caataaagct
      661 catctccatc tcccgattga aacagtcact ccccggactg tttcaacgtc ctaggacata
      721 atctcaggaa gacgcgaaaa atcatgctca aagtgaaaat cattgatctt gcgaatgaca
      781 gttgaagttg aaatagacaa ctgatgatca atgtcggtca tagaagtctt tttaattagc
      841 ttctgagcaa tcttttggtt gatgatacaa ggaatttgat gattcttctt gacgatagaa
      901 gtttcagcga ccctcatttt tgaacagtga tagcacttaa aacggccttt tctaagaaga
      961 attctagttt gaatttttta tactagaaaa tcagaaccat aatacctata taaaaatatt
     1021 atagttctaa taggatttac ccaaaagttt taaggcggtc tttttagaac ttaaatcgtt
     1081 tgaaatttag gtagcaaatt tgtttctatt ctgtcaactt ttcctatttt tatcttgttg
     1141 agcctggtat tttaacaatt caggaattga tagtgaatgt gtaaaatttt ttgttagaat
     1201 aagtttataa aaaagaaaag gagtatttga ttatgttaca aaaaatttat gagcagatgg
     1261 ctaatttcta tgatagtatt gaagaagagt atggtcctac atttggtgat aattttgact
     1321 gggaacatgt tcattttaaa tttttaattt attatttagt gagatatggc attggttgtc
     1381 gtagggattt tatcgtttac cattatcgtg ttgcttatcg tttgtatctt gaaaaattgg
     1441 taatgaatcg gggttttatt tcttgttgag gtaattttag taaatttccg aactaattta
     1501 ctcttttatg gaaagataat agtaaatagc tagtaatttt tctaaatcat tttttaatag
     1561 ttggaaatag caaatctttc tattgtttct tcttgataaa aaggcgattt tttattataa
     1621 taaattgtaa gatataattg caggtgagag tcctgccatg tatgtgagaa aggaagagcc
     1681 tgagggctca gacaagatta tgacttcagt tgttgttgta ggtacccaat ggggtgatga
     1741 aggtaaaggg aagattacag acttcctttc agcgaatgca gaagtgattg cacgttacca
     1801 aggtggtgat aatgctggtc acacgattgt gattgacggt aagaaattta agttgcactt
     1861 gattccatct gggattttct tccctgaaaa aatatctgtc attgggaatg gtatggttgt
     1921 aaatcctaaa tctcttgtaa aagagttgag ctatcttcat gaggaaggtg taacaactga
     1981 taacttgcgt atttctgatc gtgcgcatgt tattttgcct tatcatatcg agttggatcg
     2041 cttgcaagaa gaagctaagg gcgacaataa gattggtacg acaattaagg gaattggtcc
     2101 agcttatatg gacaaggctg ctcgtgttgg aattcgtatt gcagatcttt tagataaaga
     2161 tattttccgt gagcgtttag aacgtaacct tgctgaaaag aatcgtcttt ttgaaaaatt
     2221 gtatgacagt aaagcgattg ttttcgatga tatttttgaa gaatattacg aatatggtca
     2281 acaaatcaag aaatacgtga tagatacatc tgttatcttg aatgatgcgc ttgataatgg
     2341 caaacgtgtg ctttttgaag gtgcacaagg tgttatgcta gatattgacc aaggtactta
     2401 tccatttgtt acgtcatcaa accctgtagc tggtggtgtg acaattggtt ctggtgtcgg
     2461 tccaagcaag attgacaagg ttgtaggtgt atgtaaagct tatacgagtc gtgtaggaga
     2521 tggtcctttc ccaactgagt tgtttgatga agtgggagaa cgtatccgtg aagtgggtca
     2581 tgagtatggt acaacaactg gtcgtccacg tcgtgtaggt tggtttgact cagttgtgat
     2641 gcgtcatagc cgtcgtgttt ctggtattac taacctttct ttgaactcta ttgatgtttt
     2701 gagcggtttg gatactgtga aaatctgtgt ggcctatgat cttgacggtc aacgtattga
     2761 ctactatcca gctagtcttg agcaattgaa acgttgcaag cctatctatg aagagttgcc
     2821 aggttggtca gaagatatta ccggagttcg caatttggaa gatcttcctg agaatgcgcg
     2881 taactatgtt cgtcgtgtga gtgaattggt tggcgttcgt atttctactt tctcagtagg
     2941 tcctggtcgt gaacaaacaa atattttaga aagtgtttgg tcctaagaga tttttaagat
     3001 ttgtttaaga taggtcgggt atactataga cggttacaag aagacctcct aacttgttgt
     3061 aacaaatatc ctaaactttt ctttttcata ataatctccc tatagagtca ccgcattcgg
     3121 tggctttttt tgtgttggga ttcatgatat aataataaaa tcgataagta ggaaaaggaa
     3181 aagagatgta ttatacgctt gaagaaaaag aagtctttat gagggaggct ttgagagagg
     3241 ctgagattgc tcttgaacac gatgaaattc caattggttg tgtgattgtc aaagatgggg
     3301 aaatcattgg tcgtgggcat aatgcgcgtg aggaattaca gcgagcggtt atgcatgcgg
     3361 aaattatggc tatagaggat gcgaacttga gtgaggagag ctggcgcttg ctggattgca
     3421 cactttttgt gaccattgaa ccttgtgtca tgtgtagtgg ggcgattggg cttgcccgca
     3481 ttccaaatgt ggtctatggg gctaaaaacc agaaatttgg cgctgctggg agtttgtacg
     3541 atatcttgac agatgagcgt ctcaatcatc gtgtggaggt tgaaatagga attttggaag
     3601 atgaatgcgc agctattatg caggactttt ttagaaatag acggaaaaaa taattttgct
     3661 tttaaaatga ataggaatgt gatataataa atagtggagc aacagttctg cgtgaagcgg
     3721 gtcaggggag gaatccagca gccctaagcg atttgaattg tgtgctcttt tttcgtgctt
     3781 tttccgaata aataagatag aataatctag aataaatgat aatagaaaag agaaaattat
     3841 gaaaattcgt ggttttgaat tggtttcgag ttttacagat gaaaatttat tgcccaagcg
     3901 tgagacagcg catgcggctg gttacgactt aaaggttgct gtgcgtacag ttgttgcgcc
     3961 aggagagatt gtcttggttc cgacaggggt taaggcttat atgcagccga ctgaggttct
     4021 ctacctctat gatcgttctt caaatcctcg taagaagggc ttggttttaa ttaactcagt
     4081 tggggtcatt gatggagatt attatggaaa tcctggaaat gaagggcata tttttgcgca
     4141 gatgaagaat atcacagacc aagaggttgt tcttgaagtt ggggagcgta ttgtccaggc
     4201 tgtttttgct actttcttaa ttgcagatgg agatgcggct gatggcgttc gaactggtgg
     4261 atttggatcg acagggcact agaatgaaga ttatctttgt acgtcatggg gagccagatt
     4321 accgtgagtt agaggagcgt tcttatatag gatttgggat agatttggca cccttgtctg
     4381 agatgggacg gcagcaagtc cagaaattga gcaaaaatcc tttactctcg tcagctgaaa
     4441 taatcgtatc ttctgcagtc acaagagctt tagaaacggc ttcgtatgtg gtctgtgcta
     4501 cgggtcttcc tttaagagta gagcctttat tacatgaatg gcaggtctat aaaacaggaa
     4561 tagaaaactt tgaaacagct agaagactgt ttttagaaaa caagggggag ttgcttccta
     4621 atagtcctat tcaatatgag acagctacgg aaatgaagtc tcggtttcta gaatgtatgt
     4681 ctaagtatcg agaacatcag actgtggtag ttgttgctca tcgaatgctc atgcgccagt
     4741 ttgtgccaaa tgagaagatt gatttttgcc aagtgattga gtgtgagtta gagatataga
     4801 aagaggtttg tcatcgcaaa gaaaaaagcg acatttgtat gtcaaaattg tgggtataat
     4861 tcccctaaat atctgggacg ttgccccaac tgtgggtctt ggtcttcttt tgtggaagag
     4921 gttgaggttg ccgaggtcaa gaatgcgcgt gtgtccttga caggtgagaa aaccaagccc
     4981 atgaaactag ctgaggtgac ttccatcaat gtcaatcgaa ccaagacgga gatggaggaa
     5041 ttcaaccgtg tgcttggagg cggagtggta ccaggaagtc tcgtcctcat cggtggggat
     5101 cctgggattg ggaaatcaac ccttctccta caagtctcaa cccagttgtc ccaagtgggg
     5161 acagttctct atgtcagtgg ggaggagtct gcccagcaga ttaaactacg tgcagagcgc
     5221 ttaggtgata ttgatagtga gttttatctc tatgcagaga ccaatatgca gagtgttcgt
     5281 gcagaagtgg agcgtatcca gccagacttt ctcattattg attccatcca gaccatcatg
     5341 tctcctgaga tttcaggggt gcaggggtct gtttctcagg tgcgtgaagt gaccgctgaa
     5401 ctcatgcagt tggccaagac caataacatt gccatcttta tcgtaggtca tgtgaccaaa
     5461 gaaggaacct tggctgggcc tcgtatgttg gagcatatgg tggatacggt gctttacttt
     5521 gaaggggagc gtcaccacac ctttcgtatt ttgagagcgg tcaaaaatcg ttttggttcc
     5581 actaatgaga ttgggatttt tgagatgcag tcgggcggct tggttgaggt actcaatccg
     5641 agtcaagttt tcctagaaga gcgtttggat ggggcgactg gttcctccat cgttgtaacc
     5701 atggaaggga cgcgtccgat tttggcggag gttcaggctt tggtaacacc gaccatgttt
     5761 ggaaatgcca agcgtactac gacaggactt gattttaacc gtgctagctt gattatggct
     5821 gttttggaaa aacgggcagg gcttctcttg caaaatcagg atgcctatct caaatctgct
     5881 ggtggtgtta aattggatga acctgcgatt gacttggctg ttgcagttgc tattgcttcg
     5941 agctacaaag acaagccaac taatcctcag gaatgttttg tcggagaact gggcttgaca
     6001 ggagagattc ggcgcgtgaa tcgtattgag caacgcatca acgaagctgc taaactgggc
     6061 tttactaaga tttatgtacc taagaattcc ttgacaggaa tcactctgcc taaggaaatt
     6121 caggtcattg gcgtgacaac gattcaggaa gttttgaaaa aggtctttgc ataatccgtg
     6181 acaaattctc ttaaaaatga taagatagga gaaatatttg actatcaaat tttcaaggag
     6241 ggaatcgtgt cgtattttga acagtttatg caagccaatc aggcttatgt tgccctacat
     6301 gggcagttaa atctgccact taaacccaaa acaagagtag ctattgtgac ctgtatggac
     6361 tctcgtctgc acgttgcgca agctctgggc ttggcacttg gggatgctca tatcttgcgg
     6421 aatgcaggtg gtcgagtgac tgaagacatg attcgttcgc tagttatttc ccagcaacaa
     6481 atggggacaa gagagattgt ggtattgcac catacagact gtggtgctca gacctttgaa
     6541 aatgaacctt ttcaggagta tttaaaagag gaattaggtg tagatgtgtc agaccaggac
     6601 ttcttgccct tccaagatat agaagagagt gtacgcgagg atatgcaact gcttatcgag
     6661 tctcccctaa taccagacga tgtcattatc tctggtgcca tttacaatgt tgatacagga
     6721 agtatgacag tcgtagaatt ataaatactt catttagaaa gaaagtgtat gaagaaaagc
     6781 agtattttat ttatttttat tttattgcta tgtattggtt tacagtatga aaccatctac
     6841 tatacggacg gttcgaggtc aggtgcggaa tatggactaa tgggagtttc tatctttcta
     6901 gctctctttt acatgattcc ggctctttat tttctcttcc gtattgggaa aaaatgggaa
     6961 ttgccaaaga aggttttgat tctgtcttta ttgggaggga tgttcctttc aggctggttg
     7021 tctagttttg ctaatactta tatccatgat ttactggggg ttcttttccc agatagtaca
     7081 tttttaaatg cctttgaaag tgctattgtg gctcctttgg tagaagaacc cttgaaatta
     7141 ttgccacttg tttttgtttt ggctttgatt cctgtgcgaa aattaaaatt tttgttttta
     7201 cttggaattg cctccggttt gggattccaa atgattgagg atattggtta tattcgtacg
     7261 gatttgccag agggctttga ctttactatt tcgcgaattt tagagcgtat catctcagga
     7321 attgcctctc actggacttt ttcaggtcta gctgtagtag gtgtttactt gctttacaga
     7381 gcctataaag gacagaaggt tggcaagaaa cagggtctta ttgtttaact ctccttttgt
     7441 ggagttggaa acagagttgc ctttagcgat tccagtggtt acggctattg ctctctatgg
     7501 ttattatcat gcttattgct ttgttgagaa acacaatgag ttgatgacct agaatatttt
     7561 tcaaaagaat gatgcaaggg tacaaatatg gtgcccttct tttatttttg attgaaaaat
     7621 agtgcaaaaa gcgctacaat ggtagatgga aaatcttgtg aaaagcacaa gcgatacata
     7681 tataccggag gaaatcatgt ctttttctga tttaaagctg tttgcccttt cttctaataa
     7741 agaattggca gaacgtgtgg cgcaggagat tgggatagag ttggggaaat caagtgttcg
     7801 ccaattttca gatggagaga ttcaggtcaa cattgaagaa tcaatccgtg ggaaacacgt
     7861 ctttatctta caatcaacta gttcgcctgt aaatgacaat ctgcttgaaa ttttgattat
     7921 ggtagatgct ttgaagcgtg cgagtgcaga gtctgtcaat gttgtcatgc cttactatgg
     7981 gtatgcacgt caggatagaa aggcgagagc gcgtgagcca atcacttcaa aacttgtcgc
     8041 aaatatgctt gaagtagctg gagtggatcg tttattgacc atcgacttgc atgctgcgca
     8101 aattcaagga ttctttgata ttcctgtgga tcatttgatg ggtgctcctc taattgcaga
     8161 ttattttgag cggcgtggta tggttggttc tgactatgtg gttgtcagcc cggaccatgg
     8221 aggggtgact cgtgctcgta agttggcaga atttttgaaa acatctatcg ctattattga
     8281 taaacgtcgt agcgttgata agatgaatac tagtgaagtt atgaacatca tcggtaaggt
     8341 tgaaggcaag acttgtatct tgattgatga tatgattgat accgctggaa cgatttgtca
     8401 tgcggcagat gctcttgcgg aagctggtgc tgttgaagtc tatgcaagct gtacgcaccc
     8461 agttctttct ggtcctgcta cggacaatat ccaaaaatca gctattaaga aattggttgt
     8521 tttggatacc atctatctgc cagaagagcg tttgattgat aagattgagc agatttcaat
     8581 cgctcatctc ctaggggatg ctatcgtacg tattcatgaa aaacgaccac tttctccact
     8641 tttcgatatt gagaaaaaaa tttaatgacc aagcctgaga tgattctcag gttttttcgt
     8701 cttttttccg aataaatgga tagtagcggt gaatctagta aagctagatt taaaactgtg
     8761 atataataaa aagagaagaa atatgactat acgtcatcag aagtttcagc gaccatcatt
     8821 tttgaacagt gatagcactt gaatcgacgc tttctaagga gaattctagt aggtatacca
     8881 gtcgtttcaa gataaggaat tttagaaggt ttttgaaagt catatttctt caattggttt
     8941 ccgcactcag ggcaagatgg ggcgtcgtag tccagtttgg cgatgatttc cttgtgtgta
     9001 tccttattga tgatgtttaa aatctggaca ttagggtctt taatgtctag taattttgtg
     9061 ataaaatata attgttccat atgattcttt ctaatgagtt gttttgtcgc ttttcattat
     9121 aggtcatatg ggactttttt tctacacaaa ataggctcca taatatccat aggggattta
     9181 cccactatgg atattatgga gccattttac aacagtgcct caaattcaga tactgacata
     9241 cctaattgct ggctggcaac ctcagaagtc agaagacctt ggcgtactat gtctaggaag
     9301 gcaaaaagtt tcctttctgc ccttccttca actttcccac gctctagtcc ttgttcaagg
     9361 ccttcagccc tagcagtttc caaggcatag tcctgtgcta acaagacttg ttcttctcgc
     9421 atatgtagtt gattaaacat tttcctgtcc tcctcggacc agctcttgta gtccagcagt
     9481 tgatttgctt gggtaatggc tcgttgcggt tgctgggtaa agggcttgtt gccgaaaaac
     9541 tccaaccatg gcttgcgaac cttgtctttg ctggtttctc tgtatttttt aattccaaga
     9601 atgccatctt aaccagatgg ttttcctgtc cattgttggt aatcgccaat acctcacctg
     9661 ttgtgtcttc gcgcatacta aagctatgaa aagccaggtc atctgagaaa taattactat
     9721 ccacgatagc aatggcgtaa acaggagcga tgtgtttgta gctctagtga gtatcacctt
     9781 ctcgctgacg aattttttca agattttgat taacctgact gcacaggtaa gcccacaagt
     9841 gattgatgaa aaaattctga tgatggactt gaatctcaat aattacttga gtaccgttat
     9901 ccaactccgc caagacgtct atactggtat aaaaatcctg cgctgagtag ggcatggaag
     9961 gcaagacatg aatgttactt ccctccaaaa tagtcacatt tttggctggc aagtccagca
    10021 tatcgcggat aaattgacaa gtgatttctg ggttgctgaa aattttctta gcaaccaagt
    10081 cattggtcgg gctaattccc agatgtctga gaatcatcct tttcctcctt ctatagtgaa
    10141 ttgaaataag atgtgaacaa ctctatcagg aaagtcaaat taatttatag aaat
//
LOCUS       DAVLVZ010000065         9878 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000065 DAVLVZ010000000
VERSION     DAVLVZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.065"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            109..5049
                     /locus_tag="VYN77_001675"
     CDS             109..5049
                     /locus_tag="VYN77_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1559104.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVQAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLNLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLHYQGH
                     EVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSDS
                     ALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELTFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            5139..6335
                     /locus_tag="VYN77_001676"
     CDS             5139..6335
                     /locus_tag="VYN77_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1559105.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6471..6998
                     /locus_tag="VYN77_001677"
     CDS             6471..6998
                     /locus_tag="VYN77_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1559106.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7075..7431
                     /gene="glnR"
                     /locus_tag="VYN77_001678"
     CDS             7075..7431
                     /gene="glnR"
                     /locus_tag="VYN77_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1559107.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7468..8814
                     /gene="glnA"
                     /locus_tag="VYN77_001679"
     CDS             7468..8814
                     /gene="glnA"
                     /locus_tag="VYN77_001679"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1559108.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8910..9029)
                     /locus_tag="VYN77_001680"
     CDS             complement(8910..9029)
                     /locus_tag="VYN77_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1559109.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            9518..9878
                     /locus_tag="VYN77_001681"
                     /pseudo
     CDS             9518..9878
                     /locus_tag="VYN77_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3195 a         1791 c         2153 g         2739 t
ORIGIN      
        1 tgttttagga taaaaatgtg attatcatgg ttctagagtt tgataaaaaa ctacagaata
       61 taaagcgctt tcttgaaaac aacaaaatca atcttttagg aggagaaaat gaagaatcca
      121 ttttttgaaa gacgttgtcg ttacagtatt cgtaagttat cagtaggagc ctgctcgctg
      181 atgattggtg ctgttttatt tgctggtcca gccttggctg aagaaactgc agttcctgaa
      241 aatagcggag ctaatacaga gcttgtttca ggagagagtg agcattcgac caatgaagct
      301 gataagcaga atgaagggga acatgctaga gaaaacaagc tagaaaaggc agaaggagta
      361 gcgacagcat ctgaaactgc agaagcagct agcgcagcta aaccagagga aaaagcaagt
      421 gaggtggttg cagaaacacc atctgcagaa gcaaaaccta agtctgacaa ggaaacagaa
      481 gcaaagcccg aagcaactaa ccaaggggat gagtctaagc cagcagccga agctaataag
      541 actgaaaaag aagtccagcc agatgtccct aaaaatacag aaaaaacatt aaaaccaaag
      601 gaaatcaaat ttaattcttg ggaagaattg ttaaaatggg aaccaggtgc tcgtgaagac
      661 gatgctatta accgcggatc tgttgtcctc gcttcacgtc ggacaggtca tttagtcaat
      721 gaaaaagcta gcaaggaagc aaaagttcaa gccttatcaa acaccaattc taaagcaaaa
      781 gaccatgctt ctgttggtgg agaagagttc aaggcctatg cttttgacta ttggcaatat
      841 ctagattcaa tggtcttctg ggaaggtctc gtaccaactc ctgacgttat tgatgcaggt
      901 caccgtaacg gggttcctgt atacggtaca ctcttcttca actggtctaa tagtattgca
      961 gatcaagaaa gatttgctga agctttgaag caagacgcag atggtagctt cccaattgcc
     1021 cgtaaattgg tagacatggc aaagtattat ggctatgatg gctatttcat caaccaagaa
     1081 acaactggag atttggttaa acctcttgga gaaaagatgc gccagtttat gctctatagc
     1141 aaggaatatg ctgctaaggt aaaccatcca atcaagtatt cttggtatga tgccatgacc
     1201 tataactatg gacgttacca tcaagatggt ttgggagaat acaactacca attcatgcaa
     1261 ccagaaggag ataaggttcc ggcagataac ttctttgcta actttaactg ggataaggct
     1321 aaaaatgatt acactattgc aactgccaac tggattggtc gtaatcctta tgatgtattt
     1381 gcaggtttgg aattgcaaca gggtggttcc tacaagacaa aggttaagtg gaatgacatt
     1441 ttagacgaaa atgggaaatt gcgcctttct cttggtttat ttgccccaga taccattaca
     1501 agtttaggaa aaactggtga agattatcat aaaaatgaag atatcttctt tacaggttat
     1561 caaggagacc ccactggcca aaaaccaggt gacaaagatt ggtatggtat tgctaaccta
     1621 gttgcggacc gtacgccagc ggtaggtaat acttttacta cttcttttaa tacaggtcat
     1681 ggtaaaaaat ggttcgtaga tggtaaggtt tctaaggatt ctgagtggaa ttatcgttca
     1741 gtatcaggtg ttcttccaac atggcgctgg tggcagactt caacagggga aaaacttcgt
     1801 gcagaatatg attttacaga tgcctataat ggcggaaatt cccttaaatt ctctggtgat
     1861 gtagctggta agacagatca ggatgtgaga ctttattcta ctaagttaga agtaactgag
     1921 aagaccaaac ttcgtgttgc ccacaaggga ggaaaaggtt ctaaagttta tatggcattc
     1981 tctacaactc cagactacaa attcgatgat gcagatgcat ggaaagagct aaccctttct
     2041 gacaactgga caaatgaaga atttgatctc agctcactag caggtaaaac catctatgca
     2101 gtcaaactat ttttcgagca tgaaggtgct gtaaaagatt atcagttcaa cctcggacaa
     2161 ttaactatct cggacaatca ccaagagcca caatcgccga caagcttttc tgtagtgaaa
     2221 caatctctta aaaatgccca agaagcggaa gcagttgtgc aatttaaagg caacaaggat
     2281 gcagatttct atgaagttta tgaaaaagat ggagacagct ggaaattact aactggctca
     2341 tcttctacaa ctatttatct accaaaagtt agccgctcag caagtgctca gggtacaact
     2401 caagaactga aggttgtagc agtcggtaaa aatggagttc gttcagaagc tgcaaccaca
     2461 acctttgatt ggggtatgac tgtaaaagat accagcctac caaaaccact agctgaaaat
     2521 atcgttccag gtgcaacagt tattgatagt actttcccta agactgaagg tggagaaggt
     2581 attgaaggta tgttgaacgg taccattact agcttgtcag ataaatggtc ttcagctcag
     2641 ttgagtggta gtgtggatat tcgtttgacc aagccacgta ccgttgttag atgggtcatg
     2701 gatcatgcag gggctggtgg tgagtctgtt aacgatggct tgatgaacac caaagacttt
     2761 gacctttatt ataaagatgc agatggtgag tggaagctag ctaaggaagt ccgtggcaac
     2821 aaagcacacg tgacagatat cactcttgat aaaccaatca ctgctcaaga ctggcgcttg
     2881 aatgttgtca cttctgacaa tggaactcca tggaaggcta ttcgtatcta taactggaaa
     2941 atgtatgaaa agcttgatac tgagagtgtc aatattccga tggccaaggc tgcagcccgt
     3001 tctctaggca ataacaaggt acaagttggc tttgcagatg tacaggctgg agcaactatt
     3061 accgtttatg ataatccaaa ttctcaaact ccgctcgcaa ccttgaagag cgaagttgga
     3121 ggagacctag caagtgcacc attggatttg acaaatcaat ctggtcttct ttattatcgt
     3181 acccagttgc caggcaagga aattagtaat gtcctagcag tttccgttcc aaaagatgac
     3241 agaagaatca agtcagtcag cctagaaaca ggacctaaga aaacaagcta cgccgaaggg
     3301 gaggatttgg accttagagg tggtgttctt cgagttcagt atgaaggagg aactgaggac
     3361 gaactcattc gcctaactca cgcaggtgta tcagtatcag gttttgatac gcatcataag
     3421 ggagaacaga atcttactct ccaatatttg ggacaaccgg taaatgctaa tttgtcagtg
     3481 actgtcactg gccaagacga agcaagtccg aaaactattt tgggaattga agtaagtcag
     3541 gaaccgaaaa aagattacct agttggtgat agcttaaact tgtctgaagg acgctttgca
     3601 gtggcttata gcaatgacac catggaagaa cattccttta ctgatgaggg agttgaaatt
     3661 tctggttacg atgctcaaaa gactggtcgt caaaccttga cgcttcatta ccaaggccat
     3721 gaagttagct ttgatgtttt ggtatctcca aaagcagcat tgaacgatga gtacctcaaa
     3781 caaaaattag cagaagttga agctgctaag aacaaggtgg tctataactt tgcttcacca
     3841 gaagtaaaag aagctttctt gaaagcaatt gaagcggccg aacaagtgtt gaaagaccat
     3901 gaaactagca cccaagatca agtcaatgac cgacttaata aattgacaga agctcataaa
     3961 gctctgaatg gtcaagagaa atttaaggaa gaaaagacag aacttgatcg cttaacaggt
     4021 gaggttcaag aactcttggc tgccaaacca aaccatcctt cagattctgc cctagctccg
     4081 cttcttgaga aaaacaaggc cttggttgaa aaagtagatt tgagtccaga agagcttgca
     4141 acagcgaaac agagtctaaa agatctggtt gctttattga aagaagacaa gccagcagtc
     4201 ttttctgata gtaaaacagg tgttgaagta cacttctcaa ataaagagaa gactgtcatc
     4261 aagggtttga aagtagagcg tgttcaagca agtgctgaag agaagaaata ctttgctgga
     4321 gaagatgctc atgtctttga aatagaaggt ttggatgaaa aaggtcaaga tgttgatctc
     4381 tcttacgctt ctattgtgaa aatcccaatt gaaaaagata agaaagttaa gaaagtattt
     4441 ttcttacctg aaggcaaaga ggcagtagaa ttgacttttg aacaaacgga tagtcatgtt
     4501 atctttacag caccacactt tactcattat gcctttgttt atgaatctgc tgaaaaacca
     4561 caacctgcta aaccagcacc acaaaacaaa gtccttccaa aacctactta tcaaccggct
     4621 tctgatcaac aaaaggctcc taaattggaa gttcaagagg aaaaggttgc ctttcatcgt
     4681 caagagcatg aaaatgctga gatgctagtt ggggaacaac gagtcatcat acagggacga
     4741 gatggactgt taagacatgt ctttgaagtt gatgaaaacg gtcagcgtcg tcttcgttca
     4801 acagaagtca tccaagaagc gattccagaa attgttgaaa ttggaacaaa agtaaaaaca
     4861 gtaccagcag tagtagctac acaggaaaaa ccagctcaaa atacagcagt taaatcagaa
     4921 gaagcaagca aacaattgcc aaatacagga acagctgatg ctaatgaagc cctaatagca
     4981 ggcttagcca gcctcggtct tgctagttta gccttgacct tgagacggaa aagagaagat
     5041 aaagattaaa tatcgaaaaa tcttgtgaaa tctttcctta tatttccaaa gtgtgatata
     5101 atagtttcga ataaaataaa taaaggagtt tttgtaacat ggcaaaactt actgttaaag
     5161 acgttgactt gaaaggtaaa aaagtcctcg ttcgtgttga cttcaacgta ccattgaaag
     5221 atggcgtaat cactaacgat aaccgtatca cagcagctct tccaactatt aagtacatca
     5281 tcgaacaagg tggacgtgca attcttttct ctcaccttgg acgtgtgaaa gaagaagctg
     5341 ataaagctgg taaatcactt gctcctgtag cagctgactt ggcagcaaaa cttggtcaag
     5401 atgttgtttt cccaggtgtc actcgtggtg ctgaattgga agcggcaatc aacgctcttg
     5461 aagatggaca agttctcttg gttgaaaaca ctcgttacga agatgttgac ggcaagaaag
     5521 aatctaaaaa cgatcctgaa cttggtaaat actgggcatc acttggagat ggtatcttcg
     5581 taaacgatgc attcggtaca gctcaccgtg cacacgcatc taacgttggt atctcagcaa
     5641 acgttgaaaa agcagttgct ggtttccttc ttgaaaacga aattgcctac atccaagaag
     5701 cagttgaaac tccagaacgt ccattcgtgg ctatccttgg tggttcaaaa gtttcagaca
     5761 agatcggtgt tatcgaaaac ttgcttgaaa aagctgataa agtccttatc ggtggtggga
     5821 tgacttacac attctacaaa gcacaaggta tcgaaatcgg taactcactt gtagaagaag
     5881 acaaattgga tgttgcgaaa gctcttcttg aaaaagcaaa tggtaaattg atcttgccag
     5941 ttgactcaaa agaagctaac gcatttgctg gttacactga agtgcgtgac actgaaggtg
     6001 aagcagtttc tgaaggcttc cttggtcttg acatcggtcc aaaatctatc gccaaatttg
     6061 acgaagcttt gactggtgcc aaaacagttg tatggaacgg acctatgggt gtatttgaaa
     6121 acccagattt ccaagctggt acaatcggtg tgatggacgc tatcgtgaaa caaccaggag
     6181 ttaaatcaat catcggtggt ggtgactcag ctgccgcagc gattaacctt ggccgtgcag
     6241 acaagttctc atggattagt acgggtggtg gagcatcaat ggaacttctt gaaggtaagg
     6301 ttcttccagg acttgcagcc ttgacagaaa aataagattt tataaacaaa tcaaagaaga
     6361 gagggatgag agttcctctt ttcttttgct taaaataaaa acgcttcctc tcaactatta
     6421 ctcataaaaa tcaccgattt atgataaaat ggaaatagaa agttgagatt atgagttatt
     6481 ttaaaaaata taaattcgat aaatcccagt tcaaacttgg tatgcgaacc tttaaaacag
     6541 gtattgctgt ttttctagtt ctcttgattt ttggcttttt tggctggaaa ggtcttcaaa
     6601 ttggtgcttt gacagccgtt tttagcctga gggagagttt tgatgagagt gttcattttg
     6661 ggacttcgcg tattctagga aatagtatcg gtggactcta tgccttggtc ttcttcttat
     6721 taaatacctt tttccacgaa gccttttggg tgaccttggt agttgttcca atctgcacca
     6781 tgttaaccat tatgacaaat gtagccatga ataacaaagc aggggttatt ggtggtgtag
     6841 cagctatgtt aatcattacc ctatcaattc caagtggtga gacaatttcg tacgtgtttg
     6901 tgcgtgtatc agaaacgttt atgggagttt ttgtcgcaat tatcgtaaat tacgatattg
     6961 atcgtattcg tctcttttta gagaaaaaag aaaaataatg ttacatttta taacattatc
     7021 aattgacgtt tgtctttttt tagactataa tagacagaaa gaaggaaatt gtaaatgaag
     7081 gaaaaagaat ttcgccgaaa tatggctgtt tttcctattg gtagtgttat gaagttgacc
     7141 gatctatcgg cgcgtcagat tcgttattat gaagatcaag agttgatcaa gcccgatcga
     7201 aacgaaggaa atcgtcgcat gtattccttg aatgacatgg atcgtctgct tgaaatcaaa
     7261 gattatatct ctgaaggtta taatatcgct gccattaaga aaaaatatgc tgaacgtgaa
     7321 gcgaaatcca agaaagcggt tagtcagact gaagtacgtc gtgcacttca caatgaactc
     7381 ctccaacagg ggcgttttgc ttcagtacag tcaccttttg gtcgcggtta ggcaaccgca
     7441 agtagtcata gattaaggag aaaacttatg ccaatcacag ctgcagatat tcgtcgtgaa
     7501 gtcaaggaaa aaaatgttac ctttattcgt cttatgttct cagatatttt gggaaccatg
     7561 aaaaacgtcg aaattcctgc tacagatgaa cagttagata aggtcttgtc gaacaaggtt
     7621 atgtttgatg gatcttctat tgaaggtttt gtacgtatca atgagtcgga tatgtacttg
     7681 tacccggact tggatacatg gacagtcttc ccttggggag atgaaaatgg aagtgttgca
     7741 ggtctgatct gtgatgtcta tacaacagaa ggtgaaccat ttgcgggtga ccctcgtggt
     7801 aatttgaaac gagctcttcg tcacatggaa gaagttggat tcaaatcctt caaccttggt
     7861 ccagagccag aattcttcct atttaagttg gatgaaaatg gggacccaac acttgaagtg
     7921 aatgacaagg gtggctactt tgatttggca cctactgacc ttgcggacaa cacacgtcgt
     7981 gagattgtga atgtcttgac caaaatggga tttgaagtag aagcgagtca ccacgaggtt
     8041 gcggttggac agcatgagat tgactttaag tacgatgaag ttctccgtgc ttgtgataag
     8101 attcaaatct ttaagcttgt tgttaaaacc attgctcgca aacacggact ttacgcaaca
     8161 tttatggcga agccaaaatt tggtattgct ggatcaggta tgcactgtaa tatgtccttg
     8221 tttgatgcag aaggaaataa cgccttcttt gatccaaatg atccaaaagg aatgcagttg
     8281 tcagaaacag cttaccattt cctaggcggt ttgatcaagc atgcttacaa ctatactgcc
     8341 atcatgaacc caacagttaa ctcatacaaa cgtttggttc caggttatga agcgcctgtt
     8401 tacattgctt gggctggtcg taaccgttcg ccacttgtgc gcgtacctgc ttcacgtggt
     8461 atgggaactc gtcttgagtt gcgttcagtg gatccaatgg cgaaccctta cgttgctatg
     8521 gctgttcttt tggaagttgg tttgtatggt attgaaaata aaatcgaagc accagctcct
     8581 atcgaagaaa atatctacat catgacagca gaagagcgca aggaagctgg tattacagac
     8641 cttccatcaa ctcttcacaa cgctttgaaa gctttgacag aagatgaagt ggttaaagct
     8701 gctctcggag atcacatcta tactagcttc cttgaagcca aacgaatcga atgggcaagt
     8761 tatgcaacct tcgtttcaca atgggaaatt gataattatt tagaccttta ctaatactaa
     8821 tatagaagaa agattgcctg tgggtaaaca gtcacacagc agtctaagcc atcaaatacc
     8881 tataaccctt aaaatagcga tttcggtcgc tatttatttt tcgaaaaatc ctcttgactc
     8941 ttttgcgaat tagatttatc atcagacgtg tagaagaaaa ttaaggagtc actatggaaa
     9001 aattcaattt caaaaacaat atcgggcaag aaaacaagtt acttcaaatc gaaatctaca
     9061 agtttactaa cttttgcaag cttcaaaatt acacatctgt aaatattttt tcgaaagata
     9121 tatttgaagc catagtaaat tgagactaga atagtacact acatctgcta aaaatttcta
     9181 gaaattaatt taactttcct aatcgatttg tttacatctt atttcaatct actatattat
     9241 ctcaataaat gatgatggcg actgttttgt ttgtgcttgt cgatttttga atcctgatcg
     9301 agcggaagaa tatttctgcc gaggttgtca aggggaaaat cctgaagata ttgaatttta
     9361 tgatgaacaa ttacaggcag aaaaggtgga ggctttgaat attcgattgg aagtaaaaaa
     9421 attgaattat ctacaatctc atcaacaata aacctatttc aaccccatta tctataggcg
     9481 tatttttacg cctttttata tacttgaagc acataagaaa cattatggga agtttgctga
     9541 tggaagtact caagaaattg atgttcctta tgatattcct gatacttggg agtgggtgag
     9601 gataaaatca atttattgga attttgggca aaataagcca gagaaatcct ttaggtatat
     9661 agatacgtct agtattgata gaaaaaagaa cataatcaac tacaaaaatc tacaatatct
     9721 ttcacctgaa caagcgcctt cccgtgctag aaaattagtt tcgcagaata gtgtcttatt
     9781 ttcaacagtt agaccatatc taaaaaatat tgctgtagtt agagaactta aagagtattt
     9841 gatagctagt acagcattta ttgttttgga tactttac
//
LOCUS       DAVLVZ010000066         9823 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000066 DAVLVZ010000000
VERSION     DAVLVZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.066"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(195..1865)
                     /locus_tag="VYN77_001682"
     CDS             complement(195..1865)
                     /locus_tag="VYN77_001682"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1559110.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2095..2784
                     /gene="coaB"
                     /locus_tag="VYN77_001683"
     CDS             2095..2784
                     /gene="coaB"
                     /locus_tag="VYN77_001683"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1559111.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2796..3347
                     /gene="coaC"
                     /locus_tag="VYN77_001684"
     CDS             2796..3347
                     /gene="coaC"
                     /locus_tag="VYN77_001684"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1559112.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            3331..3894
                     /locus_tag="VYN77_001685"
     CDS             3331..3894
                     /locus_tag="VYN77_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1559113.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            4176..4703
                     /locus_tag="VYN77_001686"
     CDS             4176..4703
                     /locus_tag="VYN77_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1559114.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAIFVILASSFGFLMAGYPMVIVFRAFSHIFFGTLGALYLQKFPDTL
                     DKPKASWIFNFVLALVHALAEVLACVVFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            4715..5230
                     /locus_tag="VYN77_001687"
     CDS             4715..5230
                     /locus_tag="VYN77_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1559115.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYVYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGRVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFKPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(5227..5694)
                     /locus_tag="VYN77_001688"
     CDS             complement(5227..5694)
                     /locus_tag="VYN77_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1559116.1"
                     /translation="MSRAQLTILTNICLIEDLEAQRVVMQYRAPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEASDKSEFFYPRRTEDDWEKKIF"
     gene            complement(5767..6255)
                     /locus_tag="VYN77_001689"
     CDS             complement(5767..6255)
                     /locus_tag="VYN77_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1559117.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKTALTALNRFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKMLELGKPIKEKRMVFY
                     SK"
     gene            complement(6221..6784)
                     /locus_tag="VYN77_001690"
     CDS             complement(6221..6784)
                     /locus_tag="VYN77_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1559118.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQCDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(6794..8782)
                     /gene="uvrB"
                     /locus_tag="VYN77_001691"
     CDS             complement(6794..8782)
                     /gene="uvrB"
                     /locus_tag="VYN77_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1559119.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(8859..9812)
                     /locus_tag="VYN77_001692"
     CDS             complement(8859..9812)
                     /locus_tag="VYN77_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1559120.1"
                     /translation="MKNKRIFKDFQASKMNLNLYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLALFLLTLLSLVVLGQYRLESIHLNPYYLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         2917 a         2202 c         1767 g         2937 t
ORIGIN      
        1 ttgctcaaaa cactgttttg agattgtgga tagaactgac agagtcagta tcatattacc
       61 tacggcaagg tgaagctgac gtagtttgaa aagattttcg aagagtataa atcttattga
      121 tgaactgctt gcagtctgag aaaaaatgag cttggatatt atttccaaac tcacttaaag
      181 tcaatttcaa tccactagaa caagcctagc acagttccat cgctttcaac atccatgttg
      241 agagctgctg gacgttttgg aagacctggc atggtcataa catcaccagt taaggcaacg
      301 atgaagcctg cacctaattt tggtaccaat tcacgaatgg taatttcaaa gttttctggt
      361 gctccaagcg catttggatt gtctgagaaa ctgtattgag ttttagccat acaaattggc
      421 aatttgtccc aaccgttttg aacgatttga gcaatttgtg tttgagcttt cttctcaaag
      481 ttcactttgc taccacgata gatttcagtg acaatttttt caatcttttc ttggacagaa
      541 aggtcattat cgtacaaacg tttatagtta gctggatttt cagcaattgt cttaacaact
      601 gtttcggcaa gtgctactcc accttctgct ccatcagccc agacactagc caattcaact
      661 ggtacatcga ttgaggcaca gagttctttt aagactgcaa tttcagcttc tgtatcagat
      721 acaaattcgt taatagctac aactgctgga ataccgaact tacggatatt ttcaacgtgg
      781 cgtttcaagt tagcaaaacc tgcacgaact gcctctacat tttcttcagt cagagcgtct
      841 ttagccacac caccattcat cttaagggca cgaagggttg cgacaataac aactgcatct
      901 ggagatgttg gcaagtttgg tgtcttgata tcaaggaatt tctcagcacc aagatccgca
      961 ccaaaaccag cttcagtaac agtgtaatca gccaagtgaa gggctgttgt cgtcgccaaa
     1021 acagagttac agccatgagc gatattggca aatggaccac cgtgtacaaa ggcaggtgta
     1081 ccgtaaattg tctgaaccaa gtttggctta atagcatcct tcaaaatcaa agccaaggca
     1141 ccctcaacct gcaaatcacc tacagaaaca ggcgtacggt catagcgata accaataacg
     1201 atattcgcca aacgacgttt caagtcctcg atgtccgttg ccaagcaaag aattgccatg
     1261 atttctgaag caactgtaat atcaaaacca tcctcacgtg gaataccgtt tagaggacca
     1321 ccaagaccaa cagtcacatg gcggagcgca cggtcgttca agtccacaac gcgtttccag
     1381 aggatacgac gttgatcaat tcccagctca ttcccttggt gcaagtggtt gtcaatcaag
     1441 gcagaaaggg cattgttggc agttgtaata gcatgcatat ctccagtaaa gtggaggttg
     1501 atgtcttcca ttggcagaac ttgtgcatac ccaccaccag cagcaccacc cttgatcccc
     1561 atgactggac caagagacgg ttcgcggata gcaatcatgg ttttcttgcc aatcttgttc
     1621 aaggcatccg caagaccaat ggtaagcgtc gactttcctt cacctgcagg tgttgggttg
     1681 atggcagtaa ccaagatcaa tttaccgact ggattgctct caactgcacg aattttatca
     1741 aagctgagtt tagccttgta ctttccgtac aactccaaat cgtcgtaaga aataccaagt
     1801 ttctctacaa catcaacaat tggcttcaac tcaatactct gtgcgatttc aatatctgtt
     1861 ttcattcaaa attcctctaa cctcttatat gataattcat tatatcacaa aacaagattt
     1921 ttaacatcct aaaactctct aaacgttcgt aaatatctct gtttttaaga cttttagagt
     1981 cctttcttaa attttatatg gctttatagt ttgaaactat aataaatttt cgtttttacc
     2041 aaaaatttat cactttcatt ttacttaccg cttatttttg tgtacaatag tgctatgaaa
     2101 attttagtta catcgggcgg taccagtgaa gctatcgata gcgtccgctc tatcactaac
     2161 cattctacag gtcacttggg gaaaattatc acagagactt tgctttttgc agggcatgaa
     2221 gtttgtttaa ttacgacaaa acgagctctg aagccagagc ctcatcctaa cctaagtatt
     2281 cgagaaatta ccaataccaa ggaccttcta atagaaatgc aagaacgtgt tcaggattat
     2341 caggtcttga tccactcaat ggctgtttct gactacactc ctgtttatat gacagggctt
     2401 gaggaagttc aggctagctc caatctaaaa gaatttttaa gcaagcagaa tcatcaggcc
     2461 aagatttctt caactgatga ggttcaggtt ttgttcctta aaaagacacc caaaatcata
     2521 tccctagtca aggaatggaa tcctactatt catctgattg gtttcaaact gctggttgat
     2581 gttaccgaag atcatctggt tgacattgca cgaaaaagtc ttatcaagaa tcaagcagat
     2641 ttaatcatcg cgaatgacct gactcaaatt tcagcaggtc agcaccgagc tatatttgtt
     2701 gagaaaaatc agcttcaaac agtccagact aaagaagaaa ttgcagaact cctccttgaa
     2761 aaaattcaag cctatcattc ttagaaagga aaactatggc aaacattctc ttggctgtaa
     2821 cgggttcaat cgcctcttat aagtcggcag atttagtcag ttctctaaaa aaacaaggcc
     2881 atcaagtcac tgtcttaatg actcaggctg ctacagagtt tatccaacct ttgacactac
     2941 aggtactctc acagaatcct gtccacttgg atgtcatgaa agaaccctat cctgatcagg
     3001 tcaatcatat cgaacttgga aaaaaagcag atttatttat cgtggtacct gcaactgcta
     3061 acactattgc aaaactagct cacggatttg cggacaacat ggtaaccagt acagctctag
     3121 ccctaccaag tcatattccc aaactaatag ctcctgctat gaatacaaaa atgtatgacc
     3181 atccagtaac tcagaataat ctgaaaacat tagaaaccta cggctatcag ctgattgctc
     3241 ctaaggaatc cctactagct tgtggagacc acggacgagg agctttagct gacctcacaa
     3301 ttattttaga aagaataaag gaaactatcg atgaaaaaac gctctaatat tgcacccatt
     3361 gctatctttt ttgctaccat gctcgtgata cactttctga gctcacttat ctttaacctt
     3421 tttccatttc caatcaaacc gaccattgtt catattcctg tcattattgc cagcattatt
     3481 tatggtccac gagttggggt tacacttgga tttttgatgg gattacttag cttgacggtt
     3541 aacacgatta cgattctacc gacaagctac ctcttctctc ccttcgtacc aaacggaaac
     3601 atctactcag ctatcattgc cattgtccca cgtattttga ttggtttaac tccttactta
     3661 gtctataaac tgatgaaaaa caagactggt ctgattttag ctggggctct tggttccttg
     3721 acaaatacta tctttgtcct tggaggaatc ttcttcctat ttggaaatgt ttataatgga
     3781 aatatccaac ttcttctggc aaccgttatc tcaacaaatt caattgctga attggtcatt
     3841 tctgcaattc taaccctagc cattgttcca cgactacaaa ccttgaaaaa ataaaaacaa
     3901 tctccgcttt caaacctgaa ggtggagatt ttgctctata cagtttcttt ttaatgaaat
     3961 ctttactata gtaaaacgaa ataagaacag gataaatcga tcaggacagc caaatcgatt
     4021 tctaacaatg ttttagaagt agaggtggac tattctagtt tcaatctact ataactttta
     4081 actaaaaaat gatctataaa cccaagtaaa ttcttgcttt attgtctggt ttattgtacc
     4141 atactagtgt atatacagtt aaaaaggaga ttcttatgaa cacacggaaa aagacacaat
     4201 ttatgacaat gacagccctc ttaacggcta ttgcgatttt gattccaatt gttatgccct
     4261 ttaagattgt cattcctcct gcttcttaca ctttggggag ccacatcgct atttttatcg
     4321 ccatgttctt gtcacccttg atggcaattt ttgtcatcct agcctctagt tttggatttt
     4381 tgatggctgg ctatcccatg gttatagtat ttcgagcttt ttcccatatc ttttttggga
     4441 ctttgggggc tctttaccta caaaaattcc ccgatacctt agataaacca aaagcctcct
     4501 ggattttcaa ctttgtgtta gcgcttgtcc atgcccttgc tgaagtattg gcctgtgtcg
     4561 ttttttacgc aacttctggt accaatgtag aaaatatgtt ttatgttcta tttgtgctag
     4621 ttggatttgg cacaattatc catagtatgg tagactatac attagcacta gctgtctata
     4681 aagtgcttcg aaaacgccgt taaaaggaaa agctatgaca aaagatcgca aacaagctct
     4741 gctccaactc ttgaaagaag ctcctaaagc ccttaatggc caaagtttgg cggaacattt
     4801 tcatgtcaca cgtcaggtca ttgtgcagga cattgcaatt ttgagagccg atggcgctcc
     4861 tatcctatcc accaatcgtg gctacgtcta taagcaaatt gataccaatc cttatgtcca
     4921 taaacttttc aaggtgaaac atgaagttga agaaatcggt caagaactcc ttgctatcgt
     4981 tgataatggt ggacgtgttc aaaatacctt gattgaccat cccgtttatg gagaaatcga
     5041 aaccttgctg aaactgtctt gtcgccgaga cgtgcaacat tttctagaac aagtcgagca
     5101 ttctgatttt aaacctttat ctgaattgac agatggtgtc cattaccacc tagtcgaagc
     5161 cgaaacacaa caagacctcc actatatcga ggaggccttg gatcagctag gttatttagt
     5221 aaaggattag aaaattttct tttcccaatc gtcttctgta cggcgagggt agaaaaattc
     5281 agatttgtcc gatgcttcca tcatctccat caagggtagc atatcatagg ccagatccaa
     5341 atttggaatc tggtcttttt gcacccaaga aacttctcct tcatctgaag agcgaagggt
     5401 accagagaac tcagttgcct tataacaaac gacaatatag cgcccacctg tatctagtgg
     5461 ccaattttta atgccgacaa gttgaggatt ttggatagtc aaccctgttt cttcatagat
     5521 ttcacgaatg acagactctg cgaaagattc gccattttct acatggcctc ctggaaaggc
     5581 ataaccggac cagcgattag tttcaggagc gcgatactgc atcaccacgc gctgagcttc
     5641 gagatcttca atcagacaaa tatttgttaa aatcgttaat tgtgctcgag acataaattt
     5701 actcactttc taatttatta aatttttaga cttggtacca gtaccattag cttctacttt
     5761 ttaaaattat ttagaataga ataccatccg tttttccttt atgggctttc caagttcaag
     5821 cattttttct tgaccatcaa aagtaaaacc cattttttga tagaaagcaa tggctcgctt
     5881 gttatctttc aatacccata agaaaatttc agaaaaacga ttcagagcag tcaaagctgt
     5941 tttcattaac ttttgtgcta tgccttttcc ataatagtct tttaaaacat ataaagcaat
     6001 aatttcacca gcttgaatag tctcatcacg aaagttacca taactgataa aaccaactac
     6061 cttcagatca cctatcgcaa tcaatgtatt ttctggatac ttttgactaa agaatcgaca
     6121 tctttctaat gtcattgtct cctgaaattc tgcaggcaaa aggtcatcat aagcctctct
     6181 ccacgtttgc cagtgaacga gggatttacc ttctatctct tcaggagttt ccattgattt
     6241 gataataacc gtcatttatt ttctcccagt cttctctcaa aataccatat ttaatactat
     6301 caaaatattt accttgataa taacgaactt ttggaatatg agcttctttt ttcattctta
     6361 atttttcagc aagtttcatc ataccaagat ttcctgacca agttgtcaaa cccagatgct
     6421 ccaactccaa gtaatcctga aacgtcctat ctatccactg caacatagca actttcccaa
     6481 taccagtgtt ccagaatttt ttatcataaa taccaattcc caattccatc catcttgttt
     6541 gtttacatac ccaataacgc gaaacagtcc caactagttt atcatcaaca aaaataccaa
     6601 ggcgatttga gttgcttaaa atggattctg atttttgtag ttcgaattct ttaaaatttg
     6661 aaaaatactg ataatcatca taatagggag catcacactg tttccaaact ggattaagtt
     6721 gtgaataagc catttcccat aaagacatca aatcttctct ctttaacttt cttaaataaa
     6781 tcatactatt cccctaatcc aaggccttga cttccaacat catatcacgg atctgagccg
     6841 ccagttcaaa gtcaagcact tcgacggctt cttgcatttg tttttctagt ttcttgacga
     6901 gttctttgcg ctcttgtttg ttgaggctat tgatgtcgac ttccttgtcc tcttccttcg
     6961 caactgcctt ggtcacggca atcaggtcac ggatttcttt cttgattgtc tgtggtacga
     7021 taccatgctc ttcattatag gccatctgaa ttttccgacg gcgggcagtt tcatcgatag
     7081 cacgttgcat agactgggta accgtgtctg catacatgat gacatggcct tcactgttac
     7141 gagcagcacg tccaatagtc tggatgagtc cacgttcgtt gcgaaggaaa ccttccttgt
     7201 cagcatcgag aatagctacg aggctcactt caggaacgtc aattccttca cggagcaggt
     7261 taattccgac caagacatca aagacaccca agcgcaggtc acggataatc tccgtccgtt
     7321 ccaaggtctt gatatccgag tgcatgtact tgaccttaat acccatttcc ttgaagtagt
     7381 cggtcaagtc ctctgccatt ttcttggtca aagttgtgat aaaggtacgc tcattttttt
     7441 caacgcgggc attgatttca cccaagaggt catcaatctg tcccatagtc ggacggactt
     7501 ccacctctgg atccaagagt cccgttggac gaatgatttg ctcaatcact gtctcggtct
     7561 gttcattttc atagtcacca ggtgtcgctg aaacgtaaac aatctgatga acgtgactct
     7621 caaactcctc ccgacggaga ggacgattgt ccaaagcaga cggcaaacgg aaaccataat
     7681 taaccagcat ttctttacgc gaacggtctc cattgtacat gcccttgatt tgccctatgg
     7741 tcatatgact ctcgtcaatc ataatcaaga aatcatctgg gaagaagtcg agaagcgtat
     7801 aaggaggctc tccttcgctc cgtccatcca tgtggcgaga ataattttca accccattgg
     7861 tatagcccat ctcacgcaac atttcgatat catactctgt ccgctgtttc aaacgctggg
     7921 cttcaagcag tttaccttcc ttttcaaaga cagctaattg ttcttccaac tcggcctgaa
     7981 tctttgcaat ggcaacttcc atgtggtcgt cattggtcac aaagtgtgtc gctgggaaaa
     8041 tcgctaaatg atccacttct cccaacacct gacctgtcag agcctcaact tcacgaatac
     8101 ggtcaatttc gtctccaaaa aattctactc gaaaggcatg ttcatctcgg gaagctggga
     8161 aaatctctac cacatcccca cgaacgcgaa atcttccgcg ttggaaatca atatcattgc
     8221 gttcaaactg aatatcgacc aagtcattca agagtttatc acgagaaatc tctagaccag
     8281 gacggagact aacgacacta tcagcgtatt ccttgggcga acccaaacca tagatacaag
     8341 agactgaggc cacgacgata acatcattac gctccaaaag ggctgaggta gctgagtggc
     8401 gaagcttgtc aatctcgtca ttgacagaac tatccttctc aatataggta tcgctagaag
     8461 ggacataggc ctctggctgg taataatcat agtaggatac gaaatactca actgcatttt
     8521 cagggaaaaa ttccttaaac tccccataga gctgaccagc cagagtttta ttgtgggcaa
     8581 taaccagagt tggtttattg actttagaaa tgacctgact catagtatag gtcttccctg
     8641 ttccagtcgc ccccatcaga atctgagctt tttctccccc ctcaatgtta tccaccaact
     8701 gctcgatagc ttggggttga tctcctgatg gttgatattt tgatactagt ttaaattgat
     8761 tatctgtaat gtgattgatc ataagagtcc tctctccatg tgctattcct attttagcat
     8821 acttatagta ttttcacgaa gaaaaggacg gaccgaagtc acatcctttc attttctttc
     8881 tttaatttat tagcaagata gacaatcagc agtagtaata ccagacttgt cataatggaa
     8941 ccttctatgc caaaagaacc acctgatagc caatcttgat tgccttgtgg taaaaaggtc
     9001 atcagagacg ttcctgacgg ttgaccacta actaaaatcc caaagagatt accctgagca
     9061 aaattccaag caccatgaat acctgcaaca ccccaaactg tatcagtttt gagaaggtaa
     9121 agagccatgg caactccgaa taaaaagaga tttactagag atagaggggt gaggccagaa
     9181 ttgcccatat gaagcagggt aaagaacagg ctagatataa gaatagctag ttttagattg
     9241 gttcttgagg ccaattgagg aagtagccaa gcacgggcca ccacttcttc tgctgtcccc
     9301 tgtaaaatcc aaaatgggat agtaaagacg acaaaggcaa gataataagg attcaagtga
     9361 atggattcca aacgatattg acctaagacc actaaactta acaaggtcag aagaaaaagt
     9421 gccaggccta gactaaatcc tttcagaaga ttgctgaggc aattctctct ataaaatccc
     9481 aaggttcgaa taggtctttt ctcaactttt cttgtccatc tgaacacagt gttaagaata
     9541 aaaccaaagg ccagtaatcc taaaattaaa cgaaagtcac ttgttttatc cgtcaagctc
     9601 tgatgcaagg tctgcaagta gcttgcaaga ttttgaccag cctctccaaa atttctagca
     9661 agtccgatga gagctaacaa actaatacca tataaagtat aagccacaaa ctctcctatg
     9721 aagacaaaaa caaaggctaa caaggggctt gtgtaaaggt ttaaattcat ttttgaagct
     9781 tggaagtctt taaatattct tttgttcttc atatccccgt cct
//
LOCUS       DAVLVZ010000067         9788 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000067 DAVLVZ010000000
VERSION     DAVLVZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.067"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..319)
                     /locus_tag="VYN77_001693"
     CDS             complement(<1..319)
                     /locus_tag="VYN77_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1559121.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSP"
     gene            1026..2501
                     /locus_tag="VYN77_001694"
     CDS             1026..2501
                     /locus_tag="VYN77_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1559122.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(2467..3183)
                     /locus_tag="VYN77_001695"
     CDS             complement(2467..3183)
                     /locus_tag="VYN77_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1559123.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALANTGHYAFNLLNFLHEQGYKVYTCNPLLIKEFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVEKHSEYTYQLLTCYPNPQKRLEAGFDKLIEIKRLTASNSRAFCRALKSSPNHQ
                     IEIRNSLDKTIDFSYSLSFS"
     gene            3439..4299
                     /gene="speE"
                     /locus_tag="VYN77_001696"
     CDS             3439..4299
                     /gene="speE"
                     /locus_tag="VYN77_001696"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1559124.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            4296..5555
                     /locus_tag="VYN77_001697"
     CDS             4296..5555
                     /locus_tag="VYN77_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1559125.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            5555..6682
                     /gene="nspC"
                     /locus_tag="VYN77_001698"
     CDS             5555..6682
                     /gene="nspC"
                     /locus_tag="VYN77_001698"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1559126.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCHILQYVQEEVGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6679..7764
                     /gene="aguA"
                     /locus_tag="VYN77_001699"
     CDS             6679..7764
                     /gene="aguA"
                     /locus_tag="VYN77_001699"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1559127.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRSF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALGRPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7774..8649
                     /gene="aguB"
                     /locus_tag="VYN77_001700"
     CDS             7774..8649
                     /gene="aguB"
                     /locus_tag="VYN77_001700"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1559128.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8830..9639)
                     /locus_tag="VYN77_001701"
     CDS             complement(8830..9639)
                     /locus_tag="VYN77_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1559129.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         2850 a         1867 c         2183 g         2888 t
ORIGIN      
        1 caggcgaaaa tttttggtta tgatagtgga gaatcttttc ctttagttcc ttggtcaagc
       61 ttgatttctt gaccgagcgc ttgcgattgt tttcataaga ctgttgagca tagtcggcag
      121 aataaacctc tttgaagcgc ccttttccaa gacattgtcg gactgtccca cgcttgattt
      181 cagtgtggat agtttgagga gcttttccaa gtagagaggc aatttctcta tttgattttc
      241 cttctttttt ccatctttcg attaagcgac ggctatcgat tgtcaaatgt ttaccttttg
      301 tagtataatt gtcttgcatt tctgtgcctt tcttgtgttt gtggttgaac aacaagtata
      361 acacagaggt gttttcttat gcctacaaga gctatcagct agttgaacca tctaattttt
      421 aggagggctg ggtggctaac ttcattatag aactttcatt tacgaacata tagtaaaatg
      481 aaacaagaac agaacaaatc gatcaggaca gtaaaatcta tttctaataa tgttttagaa
      541 gcagaagtgt actattctag tttcaatcta ttatattttt gttttttatc aaaaaatact
      601 ttacaagttc ttaaaaacat gatatagtaa taaagcttag aaaatgagat gatgttttct
      661 agcaaatata aacccgagta aaaaatgcct acggacaggc agggttgaat gccgaagcgt
      721 ggttgaaaag ccacattatt gatagggtta aaagcctact tttataagtt gatgttagga
      781 cacttgttct aattcataaa tttttagtgt ggtgaaagca cacgtcatct tgtgaaacga
      841 tcaataaagt acgtaatatt tgctactaga gagttaggaa acatcgggaa cagacatact
      901 caacagaaac caaaataaac acgtcagaag attgcagagc aggtgaaaac ctgctctttt
      961 ttcatgagtc cacctttagt tccttagttt tcataaggtc ctaaaaatat tgaaaggagt
     1021 atgttttgaa agagttagat caaaaccaag ccccaattta tgaggccttg gtgaagttac
     1081 gcaagaaaag gattgttccc tttgatgttc caggtcacaa gcgtggacgg ggaaatccag
     1141 aacttgtcga actcttagga gaaaaatgtg taggcattga tgtcaattcg atgaaaccct
     1201 tggataattt aggccatcct atttcgatta ttcgtgatgc agaggagctg gctgcagatg
     1261 cttttggagc tagccatgcc tttctaatga ttggtggaac aacttcatcg gtgcagacta
     1321 tgattctggc aacctgcaag gcaggagata agattattct gccacgaaat gtccataaat
     1381 ctgctatcaa tgcgttggtt ctatgtggtg ccattcccat ctatatcgag atgagtgtag
     1441 atcctaagat tggtatcgct ttaggtcttg aaaatgaccg agtagcacag gccataaagg
     1501 accatccaga tgctaaggct atcctaatca acaatcctac ttactacggc atctgttcag
     1561 acctaaaggg gttgacagaa atggctcatg aagctggcat gatggtttta gtagatgaag
     1621 cccatggagc gcatttgcat ttcactggta aacttccaat ttctgctatg gatgcaggag
     1681 ctgatatggc agcagtttcc atgcataagt ctggtgggag tttgacacaa agttcgcttc
     1741 ttttaatcgg ggagcagatg aatcccgagt acgttcgtca gattataaac ctgacccagt
     1801 ctacatctgc ctcttactta ttgatggcta gtttggatat ttcacgtcgc aacttggccc
     1861 ttcgtggtaa agagtcgttt gaggaagtca ttgagctatc cgagtacgct cgtcatgaaa
     1921 tcaatgccat tggcggttac tatgcttact caaaagagtt aatagatggt gtgtcggtct
     1981 gtgattttga tgtaaccaag ttgtcagttt acactcaggg aataggctta acaggtatcg
     2041 aggtttatga cctcttgcga gacgaatacg acattcagat cgagtttggt gatatcggca
     2101 atatcttggc ctatatttcc atcggcgacc gcatccaaga catcgagcgc ttggttggtg
     2161 ctctggctga tattaagaga ctctattcac gagatgggaa ggacttaata gctggagaat
     2221 atattcagcc cgagttggtg ctgtctccac aagaagcctt ctattcagag agaaaaagtt
     2281 tgaccttaga tgagtctgtt ggacaggtct gtggagaatt tgtcatgtgc tatcctccag
     2341 ggattcctat cttggctcct ggtgaacgca ttacacgaga aattgtagac tatatccaat
     2401 tcgccaagga acgtggttgc tccctccaag ggacggaaga tccagaggtc aatcatatca
     2461 acgttattaa gagaaagaca aactataaga aaagtcaata gttttatcta aactatttct
     2521 tatttcaatt tgatgatttg gcgatgattt tagagcacgg caaaaagccc ttgaattaga
     2581 agcggtcaat cgcttaattt ctatcagctt atcaaatcct gcctcaagcc ttttctgagg
     2641 attagggtag catgtcaaga gttggtaggt atattctgaa tgcttttcaa cgattttatc
     2701 caactcagga aagatgatat caagacaacg agtgtattgt actttccaat cagactgttt
     2761 tttcttgaga cgatgaatat gtctagccag tatttttagt tctacttgcc gattatcgtg
     2821 ttgaaattgt tcacgattgg ggtcagaaag aagtttaaga gcgatgccat gagcgtcttt
     2881 cttatccgtt ttagtcttgc gaagtgataa tgatttggca aattccttga tgagcaaagg
     2941 attgcaggtg taaactttat atccttgttc atgcaggaag ttcagtagat taaaggcata
     3001 atgtccggta tttgcaagag cgatgagaca gtcttggttg agttgtcgaa gagacagatc
     3061 taagagttca aaaccagctt tattatttga aaaagtgagt ggtttaagaa cagtttttcc
     3121 tggaacattc aaggctgtaa catcgtgttt atttttagcg acatcaatgc ccacataaag
     3181 catgggagta cctccagatg tagtatttca agtctacttg gttatccacg aattttttgc
     3241 cttgttacct tagacgagat aaaacgtcta tgcgttatca aactcattac caattgaaac
     3301 aaaaaactgt ggttagagcc tttcagaaat cgtcaagcga ttggagggaa taaactaatc
     3361 cacagtggct tattccaagt ataccacttg ggctttggca gtagctaact gcgctaaata
     3421 taatataagg agaaatagat ggatttatgg ttttctgaag ttcatactcc agatgtcaaa
     3481 ttgtctctga gaacagccaa gcaactttac gctggaaaaa gtgaatggca ggatatcgaa
     3541 gtcttggata cgccagcttt tgggaaaata ctgattttaa atggccatgt cttgttctca
     3601 gatgcggatg atttcgtcta caatgaaatg accgttcacg ttcccatggc tgtccaccca
     3661 aatccaaaga aagtattggt tattgggggt ggtgacggcg gtgttgccca agtattaaca
     3721 ctctatcctg aactggaaca aatcgatatc gtggaaccgg atgagatgtt ggtcgaggtc
     3781 tgtcgtgagt atttcccaga ctttgctgca gggctagatg atcctcgtgt taccatttac
     3841 taccaaaatg ggctacgctt tttgcgaaac tgcgaagatg attacgatat tatcatcaac
     3901 gatgcgacag atccatttgg ccatacggaa ggactcttta ccaaggaatt ctacggcaat
     3961 agttatcgag ctctgaagga agacggcatc atgatttacc agcatgggag tcccttcttt
     4021 gacgaggatg agtcggcctg ccgaagcatg caccgcaagg tcaatcaatc ctttccaatc
     4081 agtcgggttt atcaggccca tattccaact agcccagctg gctattggtt gtttggattt
     4141 gcatcgaaaa aataccaccc tgtcaaagat tttgacaagg aaggttggaa aaaacgccag
     4201 cttttcacag aatactacac tgcaaactta cacgtgggat cctttatgtt gcccaagtat
     4261 gttgaggaca ttttagaaga agaggaagga aaaaaatgag tcgtttacta gttattggtt
     4321 gtgggggcgt tgcccaagtt gctatttcaa agatttgtca agatagcgaa acatttacag
     4381 agattatgat tgctagccgt accaagtcaa aatgcgatga cttgaaagcg aagctagaag
     4441 gcaaaacaag tactaaaatt gaaactgcag cacttgatgc tgacaaggtt gaagaagtga
     4501 ttgccctgat tgaaagctac aaaccagaag ctgttttgaa tgtagctctg ccttatcaag
     4561 atttaaccat tatggatgct tgtttggcaa caggtgttca ctatatcgat acagccaact
     4621 acgaagcaga agacacagaa gaccctgagt ggcgtgctat ctacgaaaaa cgttgtaagg
     4681 aacttggttt tacagcctac tttgactact catggcagtg ggcttatcaa gagaaattca
     4741 aagaagcagg cttgactgct cttcttggtt ctggttttga cccaggtgta actagtgtct
     4801 tttcagctta tgccctcaaa cactattttg atgaaatcca ttatatcgac attttagact
     4861 gtaatggcgg tgaccacggt tatccatttg caaccaactt taatccagaa attaatctcc
     4921 gtgaggtttc tgctccaggt tcttactggg aagatgggaa atgggtcgaa gtcgaagcta
     4981 tgtctatcaa gcgtgagtat gatttccctc aagttggaca aaaagatatg tatctccttc
     5041 accatgaaga aatcgaatca ttggccaaga acattccagg tgtcaaacgc attcgtttct
     5101 ttatgacttt tggtcaatct tacttgacgc acatgaaatg tcttgaaaat gttggactcc
     5161 ttcgtacgga taccattaac tttaacggcc aagaaattgt tccaattcaa tttttgaaag
     5221 ccttgcttcc agatcctgcc agtcttgggc cacgtacagt cggaaaaacc aatattggat
     5281 gtatctttac aggtgtcaaa gacggtgtta aaaagactat ctatatctac aatgtctgcg
     5341 accatcagga atgttacgca gaggttggtt cgcaagctat ttcttatacg acaggagttc
     5401 cagccatgat tgggacaaaa ttagtcatga acggaacttg gaaacaagct ggagtgtata
     5461 accttgagga gttagatcca gatccattca tggaagcttt gaatgagtat ggtttgccat
     5521 gggttgtggt tgaaaatcca caaatggtgg actaatgaag ttagaacaag taccaacacc
     5581 agcctatgtt attgacttgg ccaagttaga agctaattgc cacattctac aatatgtaca
     5641 agaagaggtc ggttgcaagg tcttgcttgc ccagaaggca tattccctct acaaaactta
     5701 tcccttgatt agccagtatc tatcaggtac gacagctagt ggactctatg aggccaaatt
     5761 ggcaagggaa gaatttcctg gtgaagtcca tgtttttgca ccagctttca aggatgagga
     5821 catggaggaa ttgcttggga taacagacca tatcgtcttt aactcagaga gacagttgcg
     5881 taagcatggt tcgcgttgtc gagaggctgg tgtcagtgtt ggtttgcgcc tcaaccctca
     5941 gtgttcaact caaggagatc acgcgctcta tgacccttgt gcaccaggtt ctcgctttgg
     6001 agttactata gacaagattc cgagtgattt gctagatttg gttgatggac ttcattttca
     6061 taccctttgc gagcagggag cagatgattt acaaacaact ttgaaagcag tagaagaaca
     6121 gtttggtccc tacttacatg aggtaaaatg gctcaatatg ggtggtggtc atcatattac
     6181 aagagaaggt tacgatgtgg atttgctgat ttcagaaatc aagcgtatcc gaaaaactta
     6241 caatcttgaa atctatatcg agcctggtga agccattgcg cttaatgcgg gttatttagc
     6301 aactgaggta ttagatattg tagaaaatgg tatggaaatc ttggttttag acgcctctgc
     6361 gacctgccat atgcctgatg tacttgagat gccctatcgt ccacctttga gaaatggctt
     6421 tgaggcacag gaaaaagccc atacctacag actttcttct aatacctgtc tgacgggcga
     6481 tgtgattggt gattatagtt ttgaaaatcc agtccaaatc ggagacagac tttattttca
     6541 agacatggcc atttattctt ttgtcaaaaa taataccttt aatggtattg gattgccaag
     6601 tctctatctc atggacgaac agggagactg tagcttactc aaagcttttg gctatcaaga
     6661 ctttaaaggg agattatcat gatggacagt ccaaaaaaat taggctatca catgccagca
     6721 gagtacgaac cccatcatgg taccctcatg atatggccga ctcgaccagg atcatggcct
     6781 tttcaaggaa aggctgctaa aagatcattt actcagatca tcgagaccat agcagaaggg
     6841 gaaagagtct atcttttggt ggagcaggcc tatctatctg aagcccaatc ctatcttgga
     6901 gacaaggttg tttatttaga cattcccacc aatgatgcct gggcgcgtga tactggccca
     6961 accattctcg tcaatgataa aggtaagaaa ttagccgtgg attgggcctt caatgcttgg
     7021 ggaggcacct atgatggtct ttatcaagat tatgaagagg atgaccaagt agccagtcgt
     7081 tttgctgagg ccttgggaag gcctgtctat gatgctaaac cttttgtact ggaaggaggc
     7141 gcaatccata gcgatggtca aggaactatt ctcgtaactg aaagttgctt gcttagtcct
     7201 ggtcgcaatc ctaacctgac taaagaggag attgaaaaca cattattaga aagtcttggt
     7261 gctgaaaaag ttatttggct tccttatggt atttatcagg atgaaaccaa tgaacacgtc
     7321 gataatgttg ctgcctttgt tggtcctgct gaggttgttt tagcttggac agatgacgaa
     7381 aatgatcccc agtatgccat gtcaaaagca gatctcgaac tcttagaaca ggaaacagat
     7441 gcaaaaggtt gtcacttcac cattcataaa ttgcctatcc ctgcagttcg acaagttgtg
     7501 acagaagaag atttgccagg ctacatctat gaagaaggag aagaaaagcg atacgcaggt
     7561 gaacgactag cagcttccta cgtaaacttt tatatcgcca acaaggctgt cttggttcca
     7621 cagtttgagg atgtaaacga ccaagtggcc ttagatatcc tcagcaagtg tttcccagac
     7681 cgtaaagttg tcggaatacc agccagagat attctcttag gtggtggcaa tatccactgt
     7741 atcacccaac aaattccaga ataggagaaa aagatgagaa atgtaagagt tgcaaccatt
     7801 cagatgcaat gcgctaagga tgtggcaaca aatatccaaa ccgcagagcg tttagtacgt
     7861 caggctgctg agcaaggagc ccaaattatt ctcttgcccg agttgtttga acatccctat
     7921 ttctgtcagg aacgtcagta tgactactac cagtatgccc aatctgtagc ggaaaatact
     7981 gccattcagc attttaaggt gattgctaag gaactacaag ttgttttacc aatcagtttc
     8041 tatgaaaaag atggtaatgt cttgtataac tctattgccg tcattgatgc agatggggaa
     8101 gtgctgggcg tttatcgaaa gacccatata ccagatgacc attattatca agaaaaattc
     8161 tatttcacgc ctggtaacac tggtttcaag gtctggaata ctcgctatgc taagattggt
     8221 atcggtatct gttgggatca atggttccct gaaacagcgc gctgtcttgc attgaatggt
     8281 gctgaattgc tcttttatcc tacagctatc ggttcagagc caattttgga tacagatagt
     8341 tgtggtcact ggcaacgtac tatgcaaggg cacgcagcag cgaatattgt tccagtcatc
     8401 gcagccaatc gttatggttt agaggaggtt actcctagtg aggaaaatgg cggacagagt
     8461 tccagtcttg acttctacgg ttcctccttt atgacggatg aaacaggagc tattctagaa
     8521 cgagctgaaa gacaagaaga agctgttctg ttagctactt atgacctaga caagggagca
     8581 agtgaacgcc taaactgggg cttgtttcga gatagaagac cagaaatgta tagacaaatt
     8641 acagattagt gtgggagaaa tgagagattc attctgctag actaacttct tattagcaac
     8701 tataagatac tatggcatct agtaaatcga tttttatgat tcgctattct tgtctattga
     8761 ttagtccgta ttttaaaata ttagcaaaaa agcaaatagc agtaacttct gtctatttgc
     8821 ttttcttttt tatagaatat atttctcaat agcacgcgca acgccgtctt cttcgttgct
     8881 tgaggtaacg gcatccgcaa gagatttgac ataatcgctg gcatttccca ttgcaatccc
     8941 aagccctgca aactggagca tttcgatatc gttattagca tcgcccatgg ccataatctc
     9001 tgaggaatca atcttcaaaa tctcagctag tcgtgaaaga gcagtagcct ttgtcgttcc
     9061 aagcggcatt gcttcataaa tgacaggctg cgaacgaact ccactgaatc gttggcaaag
     9121 ctcttcagca aaacgctgct caaaatcgtc tgtttgttct tttgttccta aaaacatacc
     9181 ttggaacatc cggaactttc cactagtcgc ttcttcaaga gaaatttcag tcaggtctga
     9241 aaatactagt ttagcatcat tttcaataac ttgattgggt ttgccaccga gaacaaaata
     9301 atgtgactcg tcaaaaagtg tcaactgaac atcactcttt tcagcaaggt catagaggta
     9361 ttcgatgtca gctggactca gttctttcca gtcaactaga ctccaatcac tggtctggtg
     9421 agttgaacaa ccgttgttaa caataatata ttcgttctgg aggtcaagct ccagtttttt
     9481 gtagtagggg aggacaccga aaagggggcg acccgtacag agaaccagtt tgacaccttt
     9541 ttcaatggct ttgtgaatag cagtaatgtg agcttgtggg atttccttgg cttcattgag
     9601 gagggtgccg tccatatcca aggctagtag tttaatcata ggtcttcctt ctttatcttt
     9661 tgctattatt atagcatatt ttggagaaga aattgataga aagcttgaga ctaattgatt
     9721 ttatagttta agatgttttg atgacaattc atgatttgaa gaggatattt ggcaaagata
     9781 tgctatac
//
LOCUS       DAVLVZ010000068         9765 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000068 DAVLVZ010000000
VERSION     DAVLVZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.068"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            276..368
                     /locus_tag="VYN77_001702"
                     /pseudo
     CDS             276..368
                     /locus_tag="VYN77_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(624..1244)
                     /locus_tag="VYN77_001703"
     CDS             complement(624..1244)
                     /locus_tag="VYN77_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1559130.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1244..1726)
                     /locus_tag="VYN77_001704"
     CDS             complement(1244..1726)
                     /locus_tag="VYN77_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1559131.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALGLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1726..2568)
                     /locus_tag="VYN77_001705"
     CDS             complement(1726..2568)
                     /locus_tag="VYN77_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1559132.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTVDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2663..3463)
                     /locus_tag="VYN77_001706"
     CDS             complement(2663..3463)
                     /locus_tag="VYN77_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1559133.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSAQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3607..4986
                     /gene="glmU"
                     /locus_tag="VYN77_001707"
     CDS             3607..4986
                     /gene="glmU"
                     /locus_tag="VYN77_001707"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1559134.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4996..5541
                     /locus_tag="VYN77_001708"
     CDS             4996..5541
                     /locus_tag="VYN77_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1559135.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELKKK"
     gene            5553..5867
                     /locus_tag="VYN77_001709"
     CDS             5553..5867
                     /locus_tag="VYN77_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1559136.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5884..6576
                     /locus_tag="VYN77_001710"
     CDS             5884..6576
                     /locus_tag="VYN77_001710"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1559137.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVFGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQKSLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6653..7144
                     /gene="rocS"
                     /locus_tag="VYN77_001711"
     CDS             6653..7144
                     /gene="rocS"
                     /locus_tag="VYN77_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1559138.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7242..7832
                     /locus_tag="VYN77_001712"
     CDS             7242..7832
                     /locus_tag="VYN77_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1559139.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7841..9604
                     /locus_tag="VYN77_001713"
     CDS             7841..9604
                     /locus_tag="VYN77_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1559140.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAMLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3008 a         1700 c         2080 g         2977 t
ORIGIN      
        1 aaaacacccc aaaagttaga ttttttctgt ctaacttttg gggggcagtt catttaacac
       61 ctgataatat gcgttttttt attttaaaga ctttttgacc agcctcattt ttttaacttg
      121 atactcagtg aaaagccaaa attaaactag gaagctagct gtaggctgct caaagaacag
      181 ctttgaggtt gcagataaaa cttgtgaggt caccaacata tataatgtga agctgacatg
      241 gtttgaatag attttagaag ggtatgagtc tggaactttt aatggataat gcaagattcc
      301 atagaatggg taagctagag ttcttatgtg aagagtttgg gcataaactt ttaccttttc
      361 ctccctactt atcttagtat agaaaagtga atctgaaata gtacataact gcttctaaaa
      421 cattcttata atatgattta aattctcaaa tcatattatt cagttcttat ttcattttgc
      481 tctacaatcc tgttgagaag acacgtgttc atatcaaaaa ggtattggca agttgcaata
      541 cctttttaca aggttctttt gtcttatttt tgtttcaact gactatatct cctatggttc
      601 tagttcagaa ggctaggcta taattatgat tgataagaag tatcattcca agtattgaga
      661 gtgaatgttt caaaatcatg ggtttctata atggtcaggc tggcatttgc tagaccgcca
      721 tctttacgaa gaagtggttc tttatagcct aggagagtac gaagactggc agtaagattg
      781 gcgccgtgtc cgacaattag aatacgctca gctggactat cttttaatga tttgataaat
      841 tggatggtcc gttgagttgt actatagagg gattcggctc cgaacattcg agtgtcaaat
      901 tgagcaagat ttgaacgaaa agcctggatt tgttgcgggt aaatagcttc caaggttgca
      961 attttcaaac cttctaactt cccaagttgc cattcacgga gattaggaac gatttctaaa
     1021 gaacaggggg tatggagttg actttggata atctcagcag atttgaccgc tcgaggtaaa
     1081 tcacttgaat aaatctgatc aaaaggaatt tccttgagat actgaccaag tcgttttagg
     1141 gtttcaatgg attcaggaag aagaggagaa tcaccgctag caccttgaaa acgaccttct
     1201 tggttccaga gggtacgacc gtggcggaca aagtagagtt tcattacttg atgtcctcca
     1261 aaatatctac aaagtctgcc tttacaaagc tagccaagtc ttgtggcgcg acgataatgc
     1321 tgtgtccgac ttcgcctgca gagacaatca tttgatccaa acctagagca attttatcga
     1381 taaaaatggg ataattgtgt ttctgacgaa ttccgacagg attattggct ccatgaatgt
     1441 aaccagttgt tttttctaag tccttttgtg gaatcatgct cactttttta ttgccagaaa
     1501 ttttagctag tttcttttca gacaagtgct gagtgatagg gacaattccg ataatcggtc
     1561 cggtcttgtc tcccaaaagc gccaaggttt tgaaaatctg atctcgttca taaccttgag
     1621 gaagctctcc ttctagggca ttgatttgaa tcccctgatg agggatagct gctttagata
     1681 ggatttgttc taccaatgtt tttttgattt taactttttt tgccattatt tatatttatc
     1741 ctccaattga ctcatccaaa taccaagcca gattcccagc gcaaagaaga aggcgatgat
     1801 gacataaccg acaagtgaaa gtcctgtgta ttggatactt tcagcgtttc ctgcatttgg
     1861 aattaagatc aaaagggtac ttgataggac gataccgatg atgaaatgat agacgcgtga
     1921 gtggtagttg tttaaggcat aatccatcaa ttttgaaaaa acgatgagag ttgcacctgc
     1981 accaattcca atcggaaaga aggttcccaa gagatcaaca gttttaaaac cagtcaacat
     2041 aggagcatag agtcccaaaa tcaaaagtaa attcgatggg ctgaggccag gaaccaagac
     2101 gccaagggcc aatagtgcgc ctgctaggac gaagttaaga aagctggcgc ttaaggttcc
     2161 aacgacaaaa tttaaggcat agagtcctaa tccagaaatg ataaaggttg tccataacca
     2221 agctaaatca atcttgtctc ggtcagattc tcgagttgat tctttgagga ggctaggaac
     2281 tgtaccgata atagctcccg caaagctcca taatacaaag acctgataat tttcaagcag
     2341 gtattcaatc gggtaggaaa ataagccgat tcccagaagc ataccgatgg caactggaat
     2401 aaagtacaaa acattttctt taaggtcttt aaagggatgg gccagaaagc caatcattcg
     2461 ttcatagatt cctaagattg ctgctagaac ccctccggaa attcctggta ggataaatcc
     2521 aagagcaatt acaatccctt taataacgcg tgctaaccat gagagcatat atttcctcca
     2581 actatttcta tttcaaaaaa tccttactat attgtatcac aatcagacac aaaaagaaaa
     2641 agcaaatgat aaacaaatgc ttttaaggtt ttaaaaaaag ttttcgaaag gtttcttctt
     2701 tattttttaa gggagagata acgttgatat ctaaatcgtg gtcaaagccg gcaatttttc
     2761 ctttagatgt gtattggtga atatcataat ctaaatcagt tttaggactg ctctccaaaa
     2821 atcctgagtc tgagccgtag gacggaatcc aaacagaggt aaacttgcct gtatcaatac
     2881 tgtgttcttc catgaagtag acaccaacgt agatgccgat gtttttagca cctagtgatg
     2941 ctagttttgc tcgaaagttt tcgacacctt cgttcatatt agacatggtt ttgtcttcca
     3001 cgtcaagcca atagtaacta gggctgtaag gagaggcagc attgtagaaa acttcggcag
     3061 ccttttccat ttcttgggca ctttttccag ctacataagc gtagacagca actgggacat
     3121 tccgcttttg aagttcagtg atatgactct tataggcctt gtctattcca ttgataaatg
     3181 aagcatcatt ttcttttgtc gtttgagcac cactgtgaac acgaacaata gcacctgaaa
     3241 tattttgtga gagggcatcg tagttgattt cctcaggacg ctgccagcca gagaggtcaa
     3301 taatcggttt gtctaagtgt ttcaaagcct gtgcttcaat ctgtgctata ttggattttg
     3361 ttttaaacga ttggctgtca ttaagtgggc gattgatgat taaaatgaac atcataatcc
     3421 ccaaaaaact aaataaaata agtggatgaa tttgttttct catatcttat aattctaccc
     3481 taaaaatcaa aaaaaatcaa aaaaatgggt taaggaagag actttagagc attttttcat
     3541 tcaagagtgc ggaatgattt gaaatatggt ataataaaag ggaatttcta cagaaaagag
     3601 aagattatgt caaattttgc cattatttta gcagcgggta aagggactcg catgaaatct
     3661 gatttgccaa aagttttgca caaggttgcg ggtatttcta tgttggaaca tgttttccgt
     3721 agtgtgggag ctatccaacc tgaaaagaca gtaacagttg taggacacaa ggcagaattg
     3781 gttgaggagg tcttggctgg acagacagaa tttgtgactc aatctgaaca gttgggaact
     3841 ggtcatgcag ttatgatgac agagcctatc ttagaaggtt tgtcaggaca caccttggtc
     3901 attgcaggag atactccttt aatcactggt gaaagcttga aaaacttgat tgatttccat
     3961 atcaatcata aaaatgtggc cactatcttg actgctgaaa cggataatcc ttttggctat
     4021 ggacgaattg ttcgtaatga caatgctgag gttcttcgta ttgttgagca gaaggatgct
     4081 acagattttg aaaagcaaat caaggaaatc aacactggaa catacgtctt tgacaacgag
     4141 cgtttgtttg aggctttgaa aaatatcaat accaataacg ctcaaggcga atactatatt
     4201 acagacgtca ttggtatttt ccgtgaaact ggtgaaaaag ttggcgctta tactttgaaa
     4261 gattttgatg aaagtcttgg ggtaaatgac cgtgtggcgc ttgcgacagc tgagtcagtt
     4321 atgcgtcgtc gcatcaatca taaacacatg gtcaacggtg ttagctttgt caatccagaa
     4381 gcaacttata tcgatattga tgttgagatt gctccggaag ttcaaatcga agccaatgtt
     4441 accttgaaag ggcaaacgaa aattggtgct gagactgttt tgacaaacgg tacttatgta
     4501 gtggacagca ctatcggagc aggagcggtc attaccaatt ctatgattga ggaaagtagt
     4561 gttgcagacg gtgtgacagt cggtccttat gctcacattc gtccaaattc aagtctgggt
     4621 gcccaagttc atattggtaa ctttgttgag gtgaaaggat cttcaatcgg tgagaatacc
     4681 aaggctggtc atttgactta tatcggaaac tgtgaagtgg gaagcaacgt taatttcggt
     4741 gctggaacta ttacagtcaa ttatgacggc aaaaacaaat acaagacagt cattggagac
     4801 aatgtctttg ttggttcaaa ttcaaccatt attgcaccag tagaacttgg tgacaattcc
     4861 ctcgttggtg ctggttcaac tattactaaa gacgtgccag cagatgctat tgctattggt
     4921 cgcggtcgtc agatcaataa agacgaatat gcaacacgtc ttcctcatca tcctaagaac
     4981 cagtaggagc ctatcatgga gtttgaagaa aaaacgctta gccgaaaaga aatctatcaa
     5041 ggaccaatat ttaaactggt ccaagatcag gttgaattac cagaaggcaa gggaactgcc
     5101 caacgggatt tgattttcca caatggggct gtctgtgttt tagcagtaac ggatgaacaa
     5161 aaacttatct tggtcaagca gtaccgcaaa gctatcgagg ctgtctctta cgaaattcca
     5221 gccggaaaat tggaagtagg agaaaacaca gcccctgtgg cagctgccct tcgtgaatta
     5281 gaggaagaaa cagcctatac agggaaatta gaactcttgt acgattttta ttcagctatt
     5341 ggcttttgta atgagaagtt aaaactatat ttagcaagcg atttgacaaa agtggaaaat
     5401 ccgcgtccgc aggatgagga tgaaaccttg gaagtccttg aagtgagctt agaagaagcg
     5461 aaagaattaa tccaatcagg tcatatttgt gatgccaaga caattatggc tgttcagtat
     5521 tgggagttga agaaaaaata gaggaggtca gtatgggtaa atctttatta acggatgaaa
     5581 tgattgaaag agctaataga ggcgaaaaaa tttcaggtcc tcctttgcta gatgataatg
     5641 aggagactaa gattttacca acctcttctt cccgttttgg ttatgccaat cctaagaatc
     5701 atggttttag ccaggaaacc ttgaagattc aggtcgaacc atctattcat aaaagccgtc
     5761 gcattgaaaa taccaagaga aatgtcttca attctaagtt gaataaaatc ttattcgcgg
     5821 tcatctttct cttgattttg cttgttttag caatgaaact tttgtaatag aaaaggaatt
     5881 gaaatgaaaa taggaattat tgctgctatg ccagaagaac tggcttatct ggtccagcat
     5941 ttagataatg cccaggagca agttgttttt gggaatacct atcatacagg aaccattgct
     6001 tctcatgaag tcgttcttgt agaaagtgga attggtaagg tcatgtctgc tatgagtgtg
     6061 gcgattttgg ctgatcattt ccaggtggat gcccttatta atacgggttc agctggggca
     6121 gtagcagaag gtatcgctgt tggggatgtc gtgattgctg acaaattagc ctatcatgac
     6181 gtggatgtca cagcttttgg ctatgcttat ggacaaatgg cgcaacaacc gctttatttc
     6241 gaatcagaca aaacctttgt tgctcaaatc caaaagagtt tatctcaatt ggaccaaaac
     6301 tggcatcttg gtttgattgc tacaggagat agttttgttg caggaaatga caagatagaa
     6361 gcgattaagt cccatttccc agaagtttta gccgtggaga tggagggggc agctattgct
     6421 caagcagcgc atgccctcaa tctcccagtc ttagtcatcc gagctatgag tgacaatgcc
     6481 aaccatgaag caaacatctt ttttgatgag tttattatcg aagctggacg tcgctctgcc
     6541 caagtcttgt tgaccttttt gaaggcttta gattaagcgg aaatttgaca gtttttctag
     6601 cttatgataa gatttaagta aagaaaagct agaaaacgtt tcagaggata ttatgagtat
     6661 tgaaatgacc gtcagtgaga ttgcagaggt cttaggatta tctcgccaag caatcaataa
     6721 ccgtgtcaaa gaattaccag aagaagacac agataaaaat gacaaagggg taacagttgt
     6781 taccagaagt ggcttgatta agctagaaga aatctataaa aaaacgattt ttgaagatga
     6841 acctgtcagt gaagatgtca aacaacgtga actgatggag attctagtgg atgagaagaa
     6901 tgcagaaatt cttcgtcttt atgaacaatt aaaagccaag gatcgtcagt tatcagaaaa
     6961 agacgagcag atgcgtatca aagaccgtca gattgctgag aaagacaaac aattagatca
     7021 gcaacaacaa ttgacccttc aagccatgaa ggatcaagaa aatcttaagc tagagctgga
     7081 ccaagcaaaa gaagaagtcc aatccactaa aaaaggcttt tttgctcgtt tatttggagg
     7141 ataatcaagg agctgtttag gttaaatgct aacctgatgg cttcttttgt ttgtgaatag
     7201 tatagttgat tagaacagag acagtagagg agaggtacag aatggaacga ttagaagatt
     7261 acattgcttt tgatttagaa tttaatcagc acgaaggaca aacacacttg attcaagtat
     7321 cggcagtgcg ttttagagat ggtcgggaaa tcgatgccta tgattcctat gttcatacca
     7381 gcgtccctct aaagagcttt atcaatggtt tgaccgggat tacagctgag accttaaaag
     7441 acgctcctcc agttgagaaa gtcttagaag aattccaaga gtttgtgggt tctttgctga
     7501 tgataggcta caatgctgct aaaagtgatt tacctatttt agcagagcat ggtttagact
     7561 atagccaaca gtacaaggtg gatttgtatg atgaagcttt tgaacgtcgg agttcagatt
     7621 tacatggtat tgccaatctc aaattgcaaa ctgttgcctc atttcttgga tttcaaggtc
     7681 agtctcataa tagtttagag gatgctcgga tgacggcgcg tgtttacgaa tccttcttag
     7741 agtcggatca agctagggaa ttgttagaga cagaaagtag cttatcaaac aatccttttg
     7801 gtggcttgga cttgtctcaa ttatttgact aggagtgcct atgaaacctg aaaaacctaa
     7861 aaaattgggt cttaaacaga tttacctcaa tcttgataaa atcctctttc tatttttctt
     7921 gatttttatg atagtggagt atttgtgggt accattcaat tcatggttgg ctagcttctt
     7981 attagatcag acaggttatc tttttatttc ctataacaat gtcttagcca tccttactca
     8041 ttctccattg attagtctag cttttttagc cttgattgta attaaccttc tggttgctta
     8101 ttttcaaata ggactcctct ttattggtgc tcgtcatctc ctctataatg aaaagcgaag
     8161 gctaattgag tatatccgca aggttttcca tgaaagtttt ggatttatga aaaagctaac
     8221 ttgcgctaaa gccttgttta tctttttcta tatagctatg ctgtttcctt ttatacggaa
     8281 aatgctgaag atttattatc ttaataaaat cgtcattcca gagtttatcc aagcttattt
     8341 agaagataga tattggatgt ggtggctgag catcctactt ctatctctta tctttctcta
     8401 tgtctctgtc agattgatgt ttgcccttcc gaagattgta tatgatcagc tgactgttcg
     8461 agaagcagtg atgtttagct tggagaaaac caagaagaga gttacttttt atgcttggaa
     8521 tttattttac atcttgctca aagcacattt attattttat cttcctttga ttcctttatt
     8581 attggctcaa actttggtag atgatatcac tcagagagag tctctgattc ttggtatttt
     8641 gaattttgtc gtgattaaaa atctctatta tatggctctg acttattttt tggttaaatt
     8701 tgtttcattt ttgacaggaa aggagctgga tatgcttcct aggagagaaa aagatcacat
     8761 cgtgcgatgg ggtgtcatga cttgtgccag tcttttcttt gccttagaag gttatattta
     8821 tctggaggct cccatggttc atctacctca acttatttct caccgagggg tttccaatgc
     8881 aaatgggatt caaaatacag tagagtcctt ggaaactaca gcacaactca aaccagactt
     8941 ggtggagacg gacgtgcagg aaacaaaaga tgggcagttt gtcatgatgc atgatgctaa
     9001 cttgaaaaat ctagcaggta tcaataaaag tcctcaagac ttaaacttgg aggagcttaa
     9061 agggattgat atttttgaaa atggctacca gactaaaatt tcaagctttg aagattatct
     9121 cagtcgagcc aacgaacttg gtcaaaaatt actaattgaa attaaaacca gtaaaaaaga
     9181 tagtccagac atgatgaacc gctttttagc ccgttatgct gcaatgctca agatttatgg
     9241 acatcaaatc cagtctttag actaccatgt tgtcgaaaaa gtaagacagt atgatgccga
     9301 actgccagtt tatttcatca tgccctacaa ttctgtcttt cctaaaacaa gagcgacagg
     9361 atatactatg gagtactcaa ccttggatga atattttgta agcaaactat ggacaacgga
     9421 tcagaaactt tatgtctgga ctgtcaatga ttcagaagct atcagcaaat ctcttcactt
     9481 aggagtagat ggagtgatta cagatgattt ggaaaaagtt cagcgggaaa tagaagtagc
     9541 ccaagaagat ccagaatata cggatttgct cttgaaaaaa gcattggaat tctttgaatt
     9601 ttagtaataa aaaagaggaa cggttgttcc tcccatgata ggtaagtatt cttgtgaatg
     9661 cttgcttttt tgtggttgca atccgtttac gagaattgtt tatattgttc ttgtttcaat
     9721 ttactttata gtgctttgaa gttggaatgc tacactataa cttat
//
LOCUS       DAVLVZ010000069         9697 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000069 DAVLVZ010000000
VERSION     DAVLVZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.069"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            13..1014
                     /locus_tag="VYN77_001714"
     CDS             13..1014
                     /locus_tag="VYN77_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1559141.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1622..1699
                     /locus_tag="VYN77_001715"
                     /pseudo
     CDS             1622..1699
                     /locus_tag="VYN77_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2245..3606
                     /gene="trpE"
                     /locus_tag="VYN77_001716"
     CDS             2245..3606
                     /gene="trpE"
                     /locus_tag="VYN77_001716"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1559142.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYIVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEVMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFEFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3603..4169
                     /locus_tag="VYN77_001717"
     CDS             3603..4169
                     /locus_tag="VYN77_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1559143.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4180..5184
                     /gene="trpD"
                     /locus_tag="VYN77_001718"
     CDS             4180..5184
                     /gene="trpD"
                     /locus_tag="VYN77_001718"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1559144.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVVLARQVIARGKALEKLRLLQEYQK
                     "
     gene            5181..5948
                     /gene="trpC"
                     /locus_tag="VYN77_001719"
     CDS             5181..5948
                     /gene="trpC"
                     /locus_tag="VYN77_001719"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1559145.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALAEERLKELYDYATVLGLEVLV
                     ETHNLAELEVAHRLGAQIIGVNNRNLTTFEVDLQTSVDLAKHFKDDCLYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVVQRIKELQIDKG"
     gene            5935..6534
                     /locus_tag="VYN77_001720"
     CDS             5935..6534
                     /locus_tag="VYN77_001720"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1559146.1"
                     /translation="MTKVKICGLSTEEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPSDVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6512..7735
                     /gene="trpB"
                     /locus_tag="VYN77_001721"
     CDS             6512..7735
                     /gene="trpB"
                     /locus_tag="VYN77_001721"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1559147.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDMTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7728..8504
                     /locus_tag="VYN77_001722"
     CDS             7728..8504
                     /locus_tag="VYN77_001722"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1559148.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIYFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8476..9135)
                     /locus_tag="VYN77_001723"
     CDS             complement(8476..9135)
                     /locus_tag="VYN77_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1559149.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWKLLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            9205..9651
                     /locus_tag="VYN77_001724"
     CDS             9205..9651
                     /locus_tag="VYN77_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1559150.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSILKLE"
BASE COUNT         2952 a         1806 c         2319 g         2620 t
ORIGIN      
        1 tggaggactg aaatgaaaaa tataaatggg aaaaaggtaa ctatttatga tattgcacgt
       61 ttatcaggtt tttctccaaa aacagtttca cgagttatta atggtggagt aaatgttaaa
      121 gaagaaactt atcaagcgat tcaaaaagtt atagaggaat tgtcgtatat tccaaatgcg
      181 tatgcaaaaa atttaactaa aaaagaagct ataaatatat tgatttctgt aaaaaaaatc
      241 gactcttttc ctttaatttg gtttcatacg ctgttagata aagtgttacg gacgtgtaaa
      301 gaatttggtg ttaatgctat agttgagtac tttggagagg aggatacaat tagtaattca
      361 attatatcaa gcacaggtag cctagtagat ggtgttattg ttttttatga aagtgtagat
      421 gatatcagaa ttcagtattt aaagaagaac catatgcctt ttcttgtttt cggtgaatct
      481 caaacgtctg gagtagtcta tgtatctaat aataattttc aagctactta tgatatgatg
      541 aaagctgtaa ctgaagaaaa atttaaaaat atgtggttgc ttatgggagg agaatcccat
      601 gttaataagg atcgtgaaag aggtgtgcgt tcttttctga atgataaaaa ttactttatg
      661 gatttaaaag tcatttatgg cttatctaca attgattcag tttattctta tgcaatgcag
      721 cacttgacta cccaaaatta tccggacatt atttttgttt caggagatga aaaggttcag
      781 ggcttgattc gtgcgtgcta tgagaaagga attcttattc cagatgatat ttctatcatt
      841 ggatttgata atattcctat ttctcagtat tatactccag ctttgtctac aattgctccc
      901 aattatgtta aattggctaa agaaatgata gaaggtgttt tagcaattat taaaggggaa
      961 agtgtcacat ctgttgaagt ttctcctaaa tttgttagga gacagagttt ctagtattgt
     1021 ttaattcttg aaaatcctag tggaaaagat ggagtgttct aaaataagtt ctgtttagcc
     1081 tccattcagt ttctattgag ttggatccaa tttctaacaa gtgggacatg ttcatgttcg
     1141 atctaaggaa gacatggtca tattcgaatg atattttcaa aaaaatcgta aaaaagtctt
     1201 gacaaagaaa aaacaaagta ttaaaataag ttcaacaaat cagaaaagta actatttttg
     1261 atcttcaggg agcctgtggt gattgtgaat aggtggttgg aagtagtgaa agtgggctga
     1321 ttttgaaaat gaatttgaaa caatgaaaat tcggtgcgca caccttacag tgcagcttgt
     1381 tgttaaacaa ggcagagatg taaagggata gtccctttat aattgaggtg gcaccgcgtt
     1441 accaacgccc tcacacggaa gtatattctg tgtgtgggct tttttctatc cgtcgtttgg
     1501 tttatctttt attagggcct taagtcgctt tgatgaactt gagttctatc tacagctcct
     1561 tgcctactac taaaagcaaa caaaaaggct ggactaatac gaaaaaagct agtaatactc
     1621 aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc tgctcaaaac actgttttga
     1681 ggttgtggat agaactgacg aagtcagctc aaaatactgt tttgaggttg tggatagaac
     1741 tgacgaagtc agctcaaaac actgttttga ggttgcagat agaactgacg aagtcagtaa
     1801 ccatacctac ggcaaggcga cgttgacgcg gtttgaagag attttcgaag agtataaagg
     1861 tgtaccgaga tgaacatctc ttcatcaatg tctctggtcg tggaggcaag gacctagctg
     1921 cgattgcaga ctactagaag ctaaaaaata atagatggaa aaattacagt tctttctctc
     1981 acagagagct tgtggttgct gaaaacaagc agagaaagct gtaaagttgg gttcatctga
     2041 aacgagagaa gaaaacatca ttctcaaggg agtgcccttt atcgcacagc ctgttacagg
     2101 agatatctga gacaggaatg agagatagga gaaatcctat aattgaggtg gcaccgcgaa
     2161 tttcgtcctc acgcaagtta ttttgcgtgg ggatttttca tacagtcgct acgaactaga
     2221 attagaactg aaagggaaat tacaatggaa cgaatcattc atggagatgt cttatcacca
     2281 atcttggctt atatgcgctt aaaggggcaa cacaaggtta tcttagagag tattccgaga
     2341 gacaaggaaa cagctcgttt ttctatccta gcctataatc ctgtttttga gattaagttt
     2401 gaaaatggag ttctttatca aaatggtcaa gtgattgatc gggatccctt ggatttcctt
     2461 tatgaagtga ttcataagag tcagcaccat tcagagctac cttttggtgg tggagccatt
     2521 ggttttgtgg gttacgatat gatttcgctt tatgaagaaa ttggtcaaat ccctgaggat
     2581 acaattggga cgccagacat gcatttcttt gtctatgaga gctatatagt ctttgaccac
     2641 aagaaggaga agatacatgt catagaggat gcactttata gtgagcgcag tcaagaggcc
     2701 ttggaaaaat ccttgaacca agtgcttgag gaattacgca ttcctgctcc aaatgaattt
     2761 gaagacttgg atttatcacc acttgacttc aaaccgcata tcgctccaca taagtttgag
     2821 gtaatggtag aaactgctcg cgacttgatt cgtaatggtg atatgttcca atgtgtactc
     2881 agccagcgtt tctcagcaga agttactgga aatccatttg aattctacag aaatctccgc
     2941 gtgactaatc cttctaatta cctttatttc tatgattttg gggattatca aatcatcgga
     3001 gccagtccag aaagtttggt ttctgtcaaa aatggcatcg tgacaaccaa tccgattgca
     3061 gggacgcgac caagaggggc tacggatgaa gaagacaagg ccttggcgac tgacctcctc
     3121 tctgatgaga aggaaacagc agaacatcga atgttggtag acttggggcg taacgatatt
     3181 ggccgcatct ctgaaacggc cagtgtccaa gttactaagt atatggaggt ggagctcttc
     3241 cgctacgtca tgcatttgac cagtgtggtc aagggacgtt tgcttgcaga actcactgcc
     3301 atggatgcct tgaaagcaac gcttcctgct ggaaccgttt ctggagcacc aaaaattcga
     3361 gctatgagac gcatctatga actggaaacg gaaaaacggg gcgtatatgc aggagcaatc
     3421 ggctacttgt ctgcgacggg tgatatggat ttggccattg ccatccgaac catgattctc
     3481 aaaaatcaaa gagcctatgt gcaggctggg gcagggattg tctacgactc tatcgcccaa
     3541 aacgaatacc aagaaaccat taacaaagca aaatctatga ctagaattgg agaactaaga
     3601 ccatgatttt attgattgac aactatgatt cttttaccta taacttggcc caatacattg
     3661 ggaattttgc agaagttcag gttctgagaa atgatgattc caagctatat gaagaagctg
     3721 aaaaagcaga tggtctggtc ttttctcctg gtcctggttg gccagttgat gctggaaaga
     3781 tggaagacat gatttgtgat tttgcaggca agaagccgat tctagggatt tgtttgggtc
     3841 accaagccat tgcagaagtc tttggtggta agctaggttt ggctccaaaa gtcatgcatg
     3901 ggaaacagag caatatcaac tttgaagcgc catctgtttt gtatcaaggt attgaggatg
     3961 gacgtgcggt catgcgttat cacagtattt tgattgagga aatgccagaa gactttgaag
     4021 tgacagctcg ttcgactgat gaccaagcca tcatggggat tcaacataaa aacctaccga
     4081 tttatggctt ccagtaccat ccagagagca ttggaacgcc agacggcttg tcttctattc
     4141 ggaattttat cgagaaggtt gtaaagtgag gaaactagga tgaaagagat tattgaaaaa
     4201 ctagcaaaat ttgaaaattt atcaggtgtg gaaatgacgg atgtcattga gcgtatcgta
     4261 actgggcgtg taactgaagc gcagattgct tctctcctct tagctctcaa gatgaagggg
     4321 gaaacacctg aagaacgcac agccattgcc caagtcatga gaggacatgc ccagcatatt
     4381 ccaactgaga ttcatgatgc catggataac tgtggtacag gtggggacaa gtctttcagt
     4441 tttaatattt ccacaactgc agcctttgtc ttggctggtg gcggtattca catggccaag
     4501 catggtaacc gctcgatttc ttctaaatct ggttccgcag atgtcctcga agccttggga
     4561 atcaatcttg atctcaaacc agctgaacta ggtaaggtct ttgataaaac tggaatcgtc
     4621 tttctcttcg ctaaaaatat gcacccagct atgaaataca tcatgccagc tcgtttggaa
     4681 ttgggaattc caacgattat gaacttgact ggtccactga ttcatccaat ggctttagaa
     4741 acacagcttc ttggaattag tcgtccagaa ctcctagaaa gtacagctca ggttttgaaa
     4801 aatatgggtc gcaaacgtgc catcgtggtt gctggaccag aagggttgga tgaagctggc
     4861 ttgaacggaa caaccaagat tgcacttctt gaaaatggcg aaatcagctt gtcaagcttt
     4921 actccagagg atttgggaat ggaaggctat gctatggaag atattcgtgg tgggaatgct
     4981 caggaaaatg cagaaatttt gcttagcgtt ctgaaaaacg aagcaagtcc attcttggaa
     5041 acgacagtct tgaatgctgg tcttggtttc tatgctaatg gtaagattga tagcatcaag
     5101 gaaggagttg tcttggcccg tcaagtgatt gctagaggca aggcccttga aaaactcaga
     5161 ctgttacagg agtaccaaaa atgagtcagg aatttttagc acgaatctta gagcagaagg
     5221 cgcgtgaggt ggagcagatg aagctggagc aaatccagcc tctgcgccag acctatcgct
     5281 tggcagaatt tttgaagaat catcaggacc gcttgcaggt aatcgctgag gtcaagaaag
     5341 ctagccctag tttgggagat atcaatctcg atgtggatat tgtgcaacag gcccagactt
     5401 atgaagaaaa cggagcagtg atgatttcgg tgttgacaga tgaagttttc tttaaaggac
     5461 atttggatta tctacgggag atttccagtc aggtagagat tccgacgctc aacaaggact
     5521 ttatcatcga tgaaaagcaa atcatccgcg ctcgcaatgc aggtgcgaca gtcatcttgc
     5581 tcattgtggc agccttggca gaagaacgtc ttaaggaact ttacgactac gcgacagtgc
     5641 ttggtctgga agtcttggtg gaaactcaca atctagctga actagaagtg gctcacagac
     5701 ttggtgccca gattatcggg gtcaataacc gcaacttgac cacctttgag gtcgacttgc
     5761 agaccagtgt agacttggct aaacatttca aagatgattg cttgtacatt tctgaatctg
     5821 ctattttcac agggcaggat gcggaacgac tagccccata ctttaacgga attttggtag
     5881 ggacagctct tatgcaggca gaaaatgtgg tccagagaat caaggagttg cagattgaca
     5941 aaggttaaaa tttgtggact atcgaccgaa gaagcggtgg aaacagccgt ttcagcagga
     6001 gccgactaca tcggttttgt ctttgcacct agtaaaagac aggtgacttt agaagaggca
     6061 gctgagttgg caaagcttat tccttcagat gtgaaaaagg ttggagtatt tgtttcacca
     6121 agtcgggtag aactgctgga agcgattgac aaagttggct tggacttggt tcaagttcac
     6181 ggtcaggtgg cagatgattt atttgagaat ttgccttgtg ccagtattca ggctgtgcag
     6241 gtagatggaa atgggcatgt ccccaattct caggcagatt atctactctt tgatgcccct
     6301 gtggcaggaa gtggccagcc ctttgattgg ggtcaactgg atacgactgg actagcacag
     6361 cccttcttta tcgcaggtgg ccttaatgaa gataatgtag taaaagcaat tcaacatttt
     6421 actccctatg cagtagatgt atcgagcgga gtggagacag atggacaaaa agatcatgaa
     6481 aagattagaa gatttataga gagggtaaag catggcatat caggaaccaa ataaagatgg
     6541 attttacgga aaattcggcg gacgttttgt cccagaaaca ttgatgacag cagttttgga
     6601 gttggagaag gcctatcgtg aaagtcaggc agacccaagt ttccaagagg aattaaacca
     6661 actcttgcgc cagtatgtgg ggcgggaaac tcctctttac tacgcaaaaa acttgaccca
     6721 gcatattggc ggagccaaga tttatctcaa acgggaagac cttaaccata caggagccca
     6781 caagattaac aatgccttag gacaagtttg gcttgccaaa cgcatgggta aaaagaaaat
     6841 tatcgcagaa accggtgctg gtcagcacgg tgtggcaact gcaactgctg cggccctctt
     6901 taacatggaa tgtaccatct acatgggtga ggaagatgtc aaacgccaag ccctcaatgt
     6961 cttccgcatg gagcttttgg gagccaaggt cgaggctgta acagatggtt cgcgcgtgct
     7021 caaggatgcg gtcaatgcag cccttcgttc atgggtggct aatatcgacg atacccacta
     7081 tatccttggt tctgccttgg ggcctcatcc atttccagaa atcgttcgtg acttccaaag
     7141 tgtcatcggt cgcgaagcta aacaacagta tcgcgatatg acaggtcaaa atctgcccga
     7201 tgctctagta gcctgtgttg gtggtggatc taatgctatt gggctcttcc atccctttgt
     7261 agaagatgag tcagtagcta tgtatggaac tgaagcagca gggcttggtg tggatacaga
     7321 gcaccacgct gctaccttga ccaagggtcg tccgggtgtt cttcacggtt ctctcatgga
     7381 tgtgctccaa gatgcccatg gtcaaattct tgaagccttc tctatctcag caggtttgga
     7441 ctatcctggt attggtccag aacattctca ctaccacgat atcaaacgtg ccagctatgt
     7501 ccctgtgaca gacgaagaag ccttggaagg attccaactc ttgtctcgtg tggaagggat
     7561 tatcccagcc ttggaatcta gccatgctat cgcttttgcg gtgaaattgg ccaaagaact
     7621 tggaccagaa aagtctatga ttgtctgcct atcaggtcgt ggggacaagg atgtggttca
     7681 agtcaaagac cgcttggaag cagatgcagc aaagaaggga gaagctcatg cctaagacac
     7741 taacagaaaa attgaatgct attaaagcag ctggaaaagg aatttttgtt ccctatatca
     7801 tggctggtga ccacgagaaa ggtttggacg gtcttgctga aacaatttac tttttagaag
     7861 atttgggtgt ctctgcaatt gaagtgggta ttcccttttc agaccctgtt gcagatggac
     7921 ctgttatcga agaagctggc ttgcgcagtt tagcccacgg gacctctacc caggctttag
     7981 ttgaaacctt gaaaaccatt gaaacagaga ttccactggt catcatgacc tacttcaacc
     8041 ccctctttca gtacggtgtg gagaactttg tcaaagatct ggctgataca gctgtgaagg
     8101 gcttgattat cccagacttg cctcatgaac atgccaactt tgtagaacca tttttggcag
     8161 atacagatat cgccttgatt cctttagtaa gcttgaccac aggaattgag cgccagaaag
     8221 agttgattga aggggcggag ggattcatct atgccgttgc catcaatgga gtgacaggga
     8281 aatcaggcaa ttaccgtgca gatttggaca agcacttggc gcaacttcat caagtggccg
     8341 acatcccagt cttgacaggt tttggtgtat ctagtcaagc cgatctggaa cgcttcaatg
     8401 cggtgtcaga tggtgttatc gtcggttcga aaatagtaaa agctctccac caaggagagc
     8461 cgattcagga ctttatcaaa caagcagtag cttaccaaaa ataatcaaac aagtagcaag
     8521 taagaggaaa ggcacgaaag gaagtctttc ctttttcttt tgcaggagaa aggccaggat
     8581 acccgtcgca gaagcaaact gaatcaagat cagtaactcc gttacgctaa agacgagagc
     8641 acaagaagct aaaaagagga aatcccctgc gcccatgcgg atatcgataa aatgagccaa
     8701 aattccaagt atgaggaagg agaccatgac cagattccag ccagaggaag ctattaggat
     8761 taggtggaaa gtcatccaga ccagtaaggg atattcctga tggtgaaagt cgtagatacc
     8821 caaggtcaaa ccagcggtga ttaggactac ttgccccaag gaaagcaatt tccaagagta
     8881 aagcagaaag aggagtccta agactaattc aaagagggca taccagacag gatagcgaac
     8941 tttgcagtag cgacagcgaa agcgattgaa gacctgtgag agaatcggaa tcaaatctaa
     9001 gggacgcaag cgagtctgac aggaatcgca gtgactggct gaactgataa tggattgctc
     9061 tggaaaacgg tcaatgacca aaccaagaaa ggaagcgaga atgctcccga cgagaaaaaa
     9121 ataaaaatca atcatactta tctattcgta aaaaatggga gaaatagtat aatggagaaa
     9181 catagataag atggtgagga aaagatgaca attcgttttg aagaaaaggt gagtatagaa
     9241 aacgctcaat ttgtatgcca gtggtccaac tccctaggca aatcctttca agaacaatgg
     9301 atgggaccaa ggattccttt tctactgacc cttcaagcct tggaaggagt cttttcaatc
     9361 tttgatgaac aagagtttgt gggatttatc cagaaaatca ggctagaaga cagcaatctt
     9421 catatcggga gattttttat caacccccag aaacaggagc aaggcttagg tagccaggct
     9481 ttaaggaaat ttgttagttt ggcctttgaa aatgaagata tagatagtat ttctctaaat
     9541 gtcttcgagg caaatcaaag agctcagaat ctttaccaaa aagaaggatt tgaaatcgtt
     9601 caaatggttg aagcacctgt acgaaaatat agtatattga aactagaata gtacacctct
     9661 acttctaaaa cattgttaga aatcgatttg actgtcc
//
LOCUS       DAVLVZ010000070         9570 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000070 DAVLVZ010000000
VERSION     DAVLVZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.070"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            342..950
                     /locus_tag="VYN77_001725"
     CDS             342..950
                     /locus_tag="VYN77_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559151.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(1400..2104)
                     /locus_tag="VYN77_001726"
     CDS             complement(1400..2104)
                     /locus_tag="VYN77_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1559152.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPISNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(2174..4000)
                     /gene="glpO"
                     /locus_tag="VYN77_001727"
     CDS             complement(2174..4000)
                     /gene="glpO"
                     /locus_tag="VYN77_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1559153.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            complement(4042..5550)
                     /gene="glpK"
                     /locus_tag="VYN77_001728"
     CDS             complement(4042..5550)
                     /gene="glpK"
                     /locus_tag="VYN77_001728"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1559154.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(5709..7136)
                     /locus_tag="VYN77_001729"
     CDS             complement(5709..7136)
                     /locus_tag="VYN77_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1559155.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSENENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            7301..8173
                     /gene="hslO"
                     /locus_tag="VYN77_001730"
     CDS             7301..8173
                     /gene="hslO"
                     /locus_tag="VYN77_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1559156.1"
                     /translation="MNKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            8160..9140
                     /gene="dusB"
                     /locus_tag="VYN77_001731"
     CDS             8160..9140
                     /gene="dusB"
                     /locus_tag="VYN77_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1559157.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(9370..>9570)
                     /locus_tag="VYN77_001732"
     CDS             complement(9370..>9570)
                     /locus_tag="VYN77_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEV1559158.1"
                     /translation="NANGSMATGWLKDGDTWYYLEASGAMKESQWFKVSDKWYYVNGS
                     GALAVNTTVGGYRVNANGKWVN"
BASE COUNT         2754 a         2187 c         1892 g         2737 t
ORIGIN      
        1 aaatacgtct atttattttt tattgttttt tattgtagag cagacccaag cgaatgaaca
       61 taggttgtaa aaagcaaatg aaaaaactgc gaggataata gcttctgtgt ctaaataacc
      121 catttcgaga cctcttttca acaacaacaa gcctgtagtt tcgataacga tcaaacataa
      181 aaatatataa aaaattttcg cattaagcat attgttaccg ctttcttttt atttggtttt
      241 attgtataat aaaagttgaa aaaaatcaaa aatacaaaag agtttccgtt atgtctaaga
      301 tgtttaaatg catgtgtttg ctagttatgt ctgttttctg tatgtctagc atttccccgt
      361 cgcttgttgt ttttgctgat gatagcttat cggcaaactc taaagttgta tctggtgagg
      421 ctcagtttga gaatggctca tcagtgcggt tcggagatac gcaagtaaat attttaagcg
      481 atgaagttct tgaggttgtc aatccagatg gaagtgtaga cactattgaa cgacgtgctg
      541 acggcgtcta tatcaatggg gcattttata tggcttatca aaagaatgaa atcgacctaa
      601 atatatcttt tagatcatac gatcctaatg tttggaatta cgtcaacaca atacatggga
      661 ataaacaagc taacacattc gctaacttca tgactggtgc tggaatcagt tatatgattg
      721 gtaggatagg tgctttattg gggggtccat ggggtgcaat aattggtgga gcatattttg
      781 gtatacaagc ttatcagtct tacttggatt cacaaagccc atatccatac tacataacaa
      841 gtacttatat ccatgttgct caaagaaaat ggaagtttat cactgaatac tatagaaact
      901 caaactatac tgggtatgta aaaaccgtaa caacctacgt gaatttctaa aatataaacc
      961 ctgcctttat atctaggcag ggtttatatt ttagttaatt gctcaagtaa tttgtaagca
     1021 taatacttgt gaaattctac tagcatatca acgatttttg ggtctaattc gttgacataa
     1081 tcaggatttg accatttcgg cacgttgctt gcaggtgctt gctttttgcc gccttttttg
     1141 ggcgttttgg cttgttgttc agctttctga ctgtagttga cctcgtggaa ttttcgtgct
     1201 tgatatgatg cgttttatta tgagaagatt tacttcattt tttgctgaaa ttgggctctt
     1261 gcccaagctc tatcaacata attgttttct aatactcaat gaaaatcaaa gagcaaacta
     1321 ggaagctagc cgcaagctgt acttgagtac ggtaaggcga cgctgacgtg gtttgaattt
     1381 gattttcgaa gagtataaac tagaaaagtg agaatacaag cacggccaag gctgcaccga
     1441 taacaggtcc cacaacagga atccaagcat aagaccagtc tccgtctccc ttgtttgaaa
     1501 ttggaaggat actgtgcatg atacgaggtc caaggtcacg cgcaggattc aaggcataac
     1561 ctgttgtccc acctagcgat agaccgatac cgacaatcaa ggtacccact gcaaaggttc
     1621 cgatacctgc ctgaaaatcg taaagaccca aagcaaagat tgtcaacacc aaaacaaagg
     1681 ttccaaggat ttcgctaatc aagtttgata cagtatcctt gatggctggt ccagtactga
     1741 aggttgccag gatattgcct gcattttctt ctgcctcata gtgaggtttg aattgcaacc
     1801 aaaccaaaat ctgacccagc atagcccctg cgaactgggc taagatataa ggcaaaacgg
     1861 aagcccaagg caagtcacct tttaaagcta caccaattgt cacagctgga tttaaatgag
     1921 ctggactgag cttgccagat acaaatactg caaccgcaac tgcaatcccc caacccatag
     1981 taatcacaat ccaacctgag ctattgctct tggttttagg aagaaccaca cctgcaacaa
     2041 caccatttcc tagaagaatc aggattaaag tccctagaaa ttctccaaat aattcattca
     2101 tcatctttct gtctccatta aaaagaaggg acgagcgaca aggaatgctg ccctccacct
     2161 cttttatttt ttcttaattt tttaattctg ctaaatcgtt gttagcgaga gctgcttcga
     2221 catcagcacg gtaagttgct ttttcttctt ctgtccagtc atagaatcgt cccatttcat
     2281 ccaaaactgg ctcaacgatg ctatccaagc tatcacgcat aaagagcata tggttggtac
     2341 gacgaagaag gaagtcaact gggctaagag ccaactcgtt gcgcattgca tagtgaaggg
     2401 acaaagtatc tgccaagctg agtcctggcg cttgttccaa gctgtgagca agtgcaaaga
     2461 ctttcggtgc atttgaaccg taaagatttg ccagatagtg agcttccttg ctatccaaac
     2521 cacgtgatac tccaagttgc gcaaaggctt cgatttctga atccacattt gctgggttca
     2581 attctccacc tgaaacaggg taagttttag aattgatcaa tttaaagcta cggtcaaatt
     2641 ctgctttgag gatgtcaacc acgcgctcca tagctccttc agccatctta cggtagtctg
     2701 tgattttacc gccagcaaga gtcaaaagac cattgtcatc acggtccaag ctagacccac
     2761 gagaaactgc agatggatcc aaatatttct cagatgtgct actttcaagc ttgctgacag
     2821 cagactcaac atcttcacgt gttttttctt tggagagata agattcaaca gtcgcaatca
     2881 agttgtcaaa gctttcatca ctgatggtac cgttatttcc accattatag tcagaggcac
     2941 tattccctgc aatcaatgga cgaagacctg cccagctgct ttcgatatca tcaatggtga
     3001 tgttggattc tgggaagcgg ttgttgacaa tgccaagtag ataatctaca tcttcttgag
     3061 ttacttttgg atgctccaaa tcacctgtgt agtctgtatc agttgtacca aagtaagtct
     3121 tgttttcacg tgggagaaca aagaccatac gaccgtcacc caaacctgtg tcgaagtaaa
     3181 ctggctgtga aaccttgatt ttgcttgaat ctactaccaa gtgaactccc ttagttgggc
     3241 gcatttgtga gaattgcgtt cccttattag acaaattacg tactttatca ctccaaggac
     3301 ctgttgtatt aataaccaga cgggccttga tttcaaacac ttggtctgtc aagagatcac
     3361 gagctacaac acctgtaatc ttgccacttt cgtcaaagag gaagccttct gccttcacgt
     3421 ggttggcaat gagggcaccg tcttggttgg cacgtttgat gttttcaatc acgagacgcg
     3481 catcgttgtt acggaagtca agatacactc cacctcctac caagccttcc ttcttcaagt
     3541 ttggctggcg ttccaagact tgatccttgc tcaaaacctt gttcgcagct ggtgtgttgc
     3601 taacacctgc caagaggtcg tacaagtcca tggctacttt aagacggaag aggctaaagg
     3661 ttgctccatc ttcatcgtaa actggtaaga gcattggatc tgattttgga atgtgtggag
     3721 cgatttgttg aaccactgca cgttcagaaa ccgtatctga gaccacttct acgtcaaatt
     3781 gtttgaggta acgaagtcct ccgtgaacca attttgttga acgactagat gttccttctg
     3841 caaagtcttg catttcaatc aaaccagtct caagaccgct agctgccgcc tgcaaggcta
     3901 caccagctcc tgtgattcct ccaccgataa tcaagaggtc cagggtacgt tcctgcattt
     3961 ttttaattga caattcacgt gttttctttg aaaattccat atttacacac tttctaaata
     4021 aatcgcttta ttctgccagt attagtcgtc tacttccgca aagacttgag ttgctttcac
     4081 agccttcttc cagcccttgt agagttgttc cttgcgagat tcgttcatag atggctcaaa
     4141 gagttctcct gtctcgttca agagtttcaa ctcgtccaag tctttccagt accctactga
     4201 caaacctgct aggaaggccg ctcctagagc tgttgtttcc aggtttttag cacgtgcaat
     4261 gtcaatgcct aaaatatccg cctggaactg catgaggaag ttgttcatgg ctgcaccacc
     4321 atccaccttc agtacttgaa tggcggtctg agtatccact tgcatggtgt cgatgatatc
     4381 acgcacttga taagcaatag attgcaaagt cgccttgata aagtcttctt tgcttgttcc
     4441 acgagtcaaa ccaaagacgg aaccacgagc attttggttc cagtatggag cgcctagacc
     4501 tgtaaaggct ggaacgacat aaacttcatc gttgttgtga gaatcacgag cgtatttttc
     4561 agattctggt gaattttcaa ccatgcgaag accgtcacga agccactgaa tagcacttcc
     4621 tgcgatgaag atagaacctt ccaaggcata ataaacctta ccgttgattc cgtaaccaat
     4681 ggttgtcaag aggttgtttt cagacaactg catctcttcc ccagtattca tgatgatgaa
     4741 agagcctgtt ccataagtat tcttaaccat acctggctca aaagccaact gtccaaagag
     4801 ggctgcttgt tggtccccag ccatacctga gattggcacc tctccaccgt agaaatggaa
     4861 tggagctgtc ttgccgtaga tttcggagtt agaacgaact tctggaagta tagccttcgg
     4921 aatgttaagg atttccaaaa tctcatcatc ccatttgagt tctttaatgt tataaagcat
     4981 ggtacgagct gcatttgagt agtcagtcac gtgagccgca ccgtcagtca atttccaaac
     5041 caaccaagta tcgatagtac caaagagcaa ttcccctttt tctgctcgct cttgagcacc
     5101 ttctacatga tccaaaatcc aacgaacctt ggtagcagag aagtaagcat caataatcaa
     5161 accagtcttt tcatggaatt tttccacata accttggctt tttagttgct cagccaaagg
     5221 tgctgtctgg cgtgactgcc aaacgatagc attgtagata ggaagtcctg ttttcttatc
     5281 ccagacaacc gttgtttcac gttggttggt aatcccgatt gcctcgattt gatttggctt
     5341 gacaccactt tcgatgaaag cacccgcaat aactgactga acagagttcc aaatttcatt
     5401 ggcattgtgc tcaacccaac ctgcctgagg gaaaatctgg gtaaactctt tttgactcga
     5461 gctaaccttt tccccttttt tgttgaaaat gatggcacga gaacttgtag ttccctggtc
     5521 aatggccatg atgtattttt cttgtgacat gtactgtcct cctgataaag atcttgaggt
     5581 gtttcctctt tacacttact agtatacaaa ataaagcgct ttcttgttta tcaacttttt
     5641 ttaattttta aaaaaatgtg aggagattgt gtgaatttca caaaaaagac ctggattaac
     5701 caagcctttt aagtgaataa taactggcga atcgctacca aatcctccat atccaagtca
     5761 ttttttaaat aatagactgg tgtctgttcc ttcttatgaa tcgggttatt ggtaaccagc
     5821 aaatcataat gccgactagg gtcataagct tcaatggtaa taaaacgatt gtattccaaa
     5881 taccgtttca aaatggctgc catccttgaa aagacaagaa aaccagatgt caaatccaga
     5941 ccaatccgca tatgctggcc accgttttca gccatatatt cgattaactg gagccattcc
     6001 cagagaatct tcttatctaa atctgtcccc tgttgaaaag taggtagagc atgaaaaatc
     6061 tcctctgctg tctctttaaa atcatgttcc atcagtaaat atggatgacg attctctaac
     6121 tggtacttgt agcgatcctg taaaatatag cccttataga gatagacttg agcacaaagc
     6181 tgactgagtt cataggtgac atggtcctct gtataatccc ccaatagttc ctccttcttc
     6241 atttcttgaa tcaattgcgt cagtaaatct gccaactgcc ctccaaaacc aagaatatac
     6301 tccatagtat gaagaggaag aatgcgatga gataggagaa aagagaagaa tatcatcatc
     6361 tcaccatcct caactcctag tggaatatgt tgcccagcaa aaaaggacgg aacctttctc
     6421 agcaaacgaa gataaaagat attgtcaaaa taccctcgca ttttctcttc tatgacttga
     6481 aactgacaag cattgacccg aagacgttgt tgactgatgt gagcccagag aaccaagtcc
     6541 aatttctgcc ccacagacaa acttgcaccg cagatttcct ctaaattggc aatctcctgt
     6601 tttctctctg gtttctgcat gtgaccttcc cattcctgac tcgaccagac ctttcggaaa
     6661 agacagaaat agaaatagcg aatctgatgc tctggacctc gccaacgtcc attttggatg
     6721 gataaatcaa attctgacag aatctgattt aaaccagcca agtgacgacc aagcgtagcc
     6781 tcgctaatca ccaattcttg agccagctga tgggctaaaa actgttggtg gtagagaaga
     6841 taaaccaaaa tctggtattt aacagcactc tccaaaaata agctccgaat atctctcccc
     6901 ttggtagctg caccgataga cagacgcaaa ttttcatttt ctgagtgaat agtcagctct
     6961 aagccactat ccaaagcctt gtcattgagc agggtgacat atttggttag ggttgctttt
     7021 gaaaatcctg tttcttccat tacagccttg acggtcgtct gagactcttg taatagaaag
     7081 gaaaggattg aaaattgacc acactcagct ttctccatca aatctcctaa atacatttgt
     7141 tacccctttc attttctacc ttaagcatag cataaatctt acaaatgctg aaataatctg
     7201 tttctctttt tattttcatg ctgatttcct ggtccattat cctgaaaatc agtaaacaca
     7261 cggctccccc tctgggcatt tttatgctaa aatagtagct atgaataaaa ttattaaaac
     7321 tatatcagaa agcggagcct ttcgtgcttt tgtccttgac agcacggaaa ccgtccgcac
     7381 tgctcaagaa aaacaccaaa cccaagctag ctcaactgta gcgcttggtc gaactcttat
     7441 cgctagccag attctcgcag ccaatgaaaa aggaaatacc aaacttacag ttaaggtgtt
     7501 gggatctagc tctctaggtg ctattatcac cgtcgctgat accaagggga acgtcaaagg
     7561 ctacgttcaa aatcctggtg ttgacatcaa aaagactgcg actggtgaag tcctagtcgg
     7621 accttttgtt ggaaatggtc aattcctcgt tatcacagac tacggtactg gaaatcctta
     7681 caactctata actcccctca tctccggaga aatcggtgaa gaccttgcct tttaccttac
     7741 tgaaagccaa caaacgcctt cagcggtcgg cctcaatgtc cttttggacg aggaagacaa
     7801 ggtcaaggtt gcaggtggtt tcctagttca agtcttgcca ggagccaaga aagaagagat
     7861 tgctcgcttt gaaaaacgca tccaagaaat gccagctatc tctactcttc tcgaaagcga
     7921 cgaccatatc gaagccctcc tcaaggctat ctacggggac gaagcctaca agcgtctttc
     7981 tgaagaagaa atccgtttcc aatgtgactg tagccatgaa cgctttatga acgctcttgc
     8041 cagccttcca agctcagact tacaggaaat gaaagaagaa gaccacgggg cagaaatcac
     8101 ttgtcaattc tgccaaacta cttacaactt tgatgaaaag gacctggagg aactcattcg
     8161 tgacaaatct taatacacct tttatgattg gcaatgttga gattcccaat cgtaccgttt
     8221 tagcgcctat ggctggcgtg accaactcag cctttcgtac catcgcaaaa gagctcggag
     8281 ctggactcgt tgtaatggaa atggtctctg acaaaggaat ccaatacaac aacgaaaaaa
     8341 ccctgcacat gcttcatatc gatgagggcg aaaaccctgt ctctatccaa ctttttggta
     8401 gcgatgaaga cagcctagca cgcgcagcag aattcatcca agaaaacacc aagaccgata
     8461 tcgtcgatat caacatgggc tgccctgtca acaaaatcgt gaagaacgaa gctggagcta
     8521 tgtggctcaa ggatcctgac aagatctact ctatcatcaa caaggttcag tctgtccttg
     8581 atatccccct tactgtcaaa atgcgtaccg gatgggcgga cccatctctg gcagtagaaa
     8641 atgccctcgc tgctgaagct gcaggtgttt ctgccctcgc catgcatggc cgtacccgtg
     8701 aacaaatgta tactggccac gcagaccttg agacccttta caaggttgcc caagctctaa
     8761 ccaagattcc attcatcgcc aacggtgata tccgtactgt ccaagaagcc aagcaacgca
     8821 tcgaagaagt tggtgctgac gcagtcatga ttggccgagc tgccatggga aatccttacc
     8881 tcttcaacca aatcaaccat tactttgaaa caggagaaat cctacctgat ttgacctttg
     8941 aagacaagat gaagatcgcc tacgaacact tgaaacgatt gattaacctc aaaggagaaa
     9001 acgtcgccgt tcgtgaattc cgcggcctcg ctcctcacta tctccgtgga acatctggcg
     9061 ctgccaaact ccgtggagcc atttcacaag ctagcaccct ggcagagatt gaagctctct
     9121 tgcaattgga gaaggcttaa tagtttaaaa cccgtaactc tcttaaagag tctcttgagt
     9181 gccgccattc tttcctcatc aaaaaaactc cgtagtctct agcgccataa atccggctag
     9241 agattacaga gttatttttt aatctaaact tcctacaagg gtatagtatc tactgaaagg
     9301 atatggtatt tgtagggaat actttaaaga gttcttacag gaagccagca ttaacgagtt
     9361 acattaggtt tagtttaccc atttaccatt ggcattgact ctatagccac ctacagttgt
     9421 gttgactgca agggcacctg agccattgac atagtaccat ttgtctgata ctttgaacca
     9481 ttggctttct ttcatagccc ctgatgcttc aagatagtac catgtatctc catctttcag
     9541 ccaacctgtt gccatcgaac cgttagcatt
//
LOCUS       DAVLVZ010000071         9343 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000071 DAVLVZ010000000
VERSION     DAVLVZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.071"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            488..1531
                     /locus_tag="VYN77_001733"
     CDS             488..1531
                     /locus_tag="VYN77_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV1559159.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1698..3785
                     /gene="topA"
                     /locus_tag="VYN77_001734"
     CDS             1698..3785
                     /gene="topA"
                     /locus_tag="VYN77_001734"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1559160.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3891..4250
                     /locus_tag="VYN77_001735"
     CDS             3891..4250
                     /locus_tag="VYN77_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1559161.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4343..4975
                     /locus_tag="VYN77_001736"
     CDS             4343..4975
                     /locus_tag="VYN77_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1559162.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            5019..5675
                     /locus_tag="VYN77_001737"
     CDS             5019..5675
                     /locus_tag="VYN77_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1559163.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5694..6326
                     /locus_tag="VYN77_001738"
     CDS             5694..6326
                     /locus_tag="VYN77_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV1559164.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6354..6788
                     /locus_tag="VYN77_001739"
     CDS             6354..6788
                     /locus_tag="VYN77_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEV1559165.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6800..7837
                     /gene="leuB"
                     /locus_tag="VYN77_001740"
     CDS             6800..7837
                     /gene="leuB"
                     /locus_tag="VYN77_001740"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV1559166.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VEWIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIYGSA
                     PDIAGQGIANPISMILSVAMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7834..8103
                     /locus_tag="VYN77_001741"
     CDS             7834..8103
                     /locus_tag="VYN77_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1559167.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8107..8466
                     /gene="leuD"
                     /locus_tag="VYN77_001742"
     CDS             8107..8466
                     /gene="leuD"
                     /locus_tag="VYN77_001742"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1559168.1"
                     /translation="MVGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSKWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8685..8828
                     /locus_tag="VYN77_001743"
     CDS             8685..8828
                     /locus_tag="VYN77_001743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559169.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            8847..9101
                     /locus_tag="VYN77_001744"
     CDS             8847..9101
                     /locus_tag="VYN77_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559170.1"
                     /translation="MLLGCILPWKPEAFEKLKAYGDGREELMTDVRGTSCFVIKFGKA
                     GEQLAAKLWEEGKMVYASYASMTKRLKLAMRARCNGVYGR"
     gene            9091..9252
                     /locus_tag="VYN77_001745"
     CDS             9091..9252
                     /locus_tag="VYN77_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559171.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQVEYY"
BASE COUNT         2880 a         1646 c         2084 g         2733 t
ORIGIN      
        1 caaggtgcac tgttatagat tcaatacact ataatttagt gtaattgaga aaggagaaat
       61 gattgtgatt gatgttggct aggttatgtt caatgattcc taccgtctca aatcttgtca
      121 gtaaggaaaa ataaattctt caaaagtaga gattacaagg cttgtttaag aaagaattca
      181 aagaccttga caaataaaaa taaaatggtt attataagaa atggtctaaa atagatgatg
      241 atacttttcg aaaatctttt tacgtcagct cagctttgcc ttgctgtgtt ttgagcaagc
      301 tacggttagc ttccgagttt gattttcatt tactagaaat gaaactgatg agagatatca
      361 gtagacattt gagtcaggat attatggaaa atgataaaaa gagctcgtga gattggcata
      421 tcagactact aaagtattga gtttgttagg attttagcga ctagttagct gggaaaggaa
      481 gatatttgtg acaaataata aactgtattc gttgatagaa tttagaaata aaatatatga
      541 agaattagaa ctttccagaa gtgatttagc gattttacta tgtgccatgc ttatcgcctc
      601 tatcggatta aatatggatt cgactcccgt gattattgga gccatgttaa tctctccttt
      661 gatgacacct attctgggag tggggctctc tctagctata tttgatttta aattgttaag
      721 aaaatctttt aaaatattag ctattcaaat tcttgccagt ctaatagctt caacacttta
      781 tttttatctt tctcccattt cgtatgctag ttcggagatt gttgctagaa cctctccgac
      841 tatttgggat gttctcattg cttttgtagg agggatagca ggtattattg gtgctaggaa
      901 aaaagagacc aataatattg ttcctggtgt tgctattgca accgccttga tgcctcctct
      961 ttgtacagta ggttatgcta ttgcttctgc taatctaaaa tttatcatag gctcctctta
     1021 cctattcctc atcaattgta gctttattgt cattgcgact tatataggtg ttaggttgat
     1081 gatggttaag aaacattatt ttaaagataa tgaagaagac tctaaaatgc gtaggatttt
     1141 gcttctagtt gctgttttgc tgatgattcc gagtttcatc tctgcaacga ctttagtgag
     1201 agaaacgttg aaaaaagagt cccttaagaa atttatatca gagcagtttc aggggtataa
     1261 tattttgaaa aaaacctatt ctaaaaagac tcatacccta aagctaacta tttcaggaaa
     1321 ttatttgaca gaagaagaac tcgatatgat ttcaagtaag agaggtgact atggtttaag
     1381 tgatgtttct gttcaagttt cacaattgtc tgattcagaa caacttagta aggaagagct
     1441 ggtggagtat ttcttccagt atatcaagga taaggaagca aaagaaaagg agaaagccaa
     1501 taaatttgat acagagtctg aggagcaata atttcttgag aatagctggt ttctctcgtg
     1561 agtcttctat gtatatcaaa ggaagactga ggttttaagt ataaaacttt tcttctatta
     1621 tagtagctaa gtcttgacag ggttcaaaaa atggtttaca ctttataaag tttattactt
     1681 tgaaaaggtg tgatactgtg gctacggcaa caaaaaagaa aaaatcaaca gttaaaaaaa
     1741 atctagtcat cgtggagtcg cctgctaagg ccaagacgat tgaaaaatat ctaggcagaa
     1801 actacaaggt tttagccagt gtcggacata tccgtgattt gaagaaatcc agtatgtccg
     1861 tcgatattga aaataattat gaaccgcaat atatcaatat ccgaggaaaa ggccctctta
     1921 tcaatgactt gaaaaaagaa gctaaaaaag ctaataaagt ttttctcgcg agtgacccgg
     1981 accgtgaagg agaagcgatt tcttggcatt tggcccatat tctcaacttg gatgaaaatg
     2041 atgccaaccg tgtggtcttc aatgaaatca ccaaggatgc agtcaaaaat gcttttaaag
     2101 aacctcgtaa gatcgatatg gacttggtcg atgcccaaca agctcgtcgg atcttggatc
     2161 gcttggtagg gtattcgatt tcgcctattt tgtggaagaa ggtcaagaag ggcttgtcag
     2221 caggtcgcgt tcagtccatt gcccttaaac tcatcattga ccgtgaaaat gaaatcaatg
     2281 ccttccagcc agaagaatac tggacagttg atgctgtctt taaaaaggga accaaacaat
     2341 ttcatgcttc cttctatgga gtagatggta aaaagatgaa actgaccagc aatgacgaag
     2401 tcaaggaagt cttgtctcgt ctgactagta aagacttttc agtagatcag gtggataaga
     2461 aagagcgcaa gcgcaatgct cctttaccct ataccacttc atctatgcag atggatgctg
     2521 ccaataaaat caatttccgt actcgaaaaa ccatgatggt tgcccaacag ctctatgaag
     2581 gaattaatat cggttctggt gttcaaggtt tgattaccta tatgcgtacc gattcgactc
     2641 gtatcagtcc tgtagcgcaa aatgaggcgg caagcttcat tacggatcgt tttggtagta
     2701 agtattctaa gcacggtagc aaggtcaaaa acgcatcagg tgctcaggat gcccatgagg
     2761 ctattcgtcc gtcaagtgtc tttaatacac cagaaagcat cgctaagtat ctggacaagg
     2821 atcagctcaa gctatatacc cttatctgga atcgttttgt ggctagccag atgacagcgg
     2881 ccgtttttga taccatggct gttaaattgt ctcaaaaagg ggttcaattt gctgccaatg
     2941 gtagtcaggt taagtttgat ggttatcttg ccatttataa tgattctgac aagaataaga
     3001 tgttaccgga catggttgtt ggagatgtgg tcaaacaggt caatagcaaa ccagagcaac
     3061 atttcaccca accgcctgcc cgttattctg aagcaacact gattaaaacc ttagaggaaa
     3121 atggggttgg acgtccatca acctacgcgc caaccattga aaccattcag aaacgttatt
     3181 atgttcgcct ggcagccaaa cgttttgaac cgacagagtt gggagaaatt gtcaataagc
     3241 tcatcgttga atatttccca gatatcgtaa acgtgacctt cacagctgaa atggaaggta
     3301 aactggatga tgtcgaagtt ggaaaagagc agtggcgacg ggtcattgat gccttttaca
     3361 aaccattctc taaagaagtt gccaaggctg aagaagaaat ggaaaaaatc cagattaagg
     3421 atgaaccagc tggatttgac tgtgaagtgt gtggcagtcc aatggtcatt aaacttggtc
     3481 gttttggtaa attctacgct tgtagcaatt tcccagattg ccgtcatacc caagcaatcg
     3541 tgaaagagat tggtgttgag tgtccaagct gtcatcaggg acaaattatt gagcgaaaaa
     3601 ccaagcgtaa tcgcctattc tatggttgca atcgctatcc agaatgtgaa tttacctctt
     3661 gggacaagcc tgttggtcgt gactgtccaa aatgtggcaa cttcctcatg gagaaaaaag
     3721 tccgtggtgg tggcaagcag gttgtttgta gcaaaggcga ctacgaggaa gaaaagatta
     3781 aataagagga ggggcccgaa agtaaatttc aggctccttt tggttagaac ttgacaaaat
     3841 acatcatttt atgcgaaact agaagaagat tattttaact aggagaagtt atgcgtctta
     3901 tctatctgat aattggtttt ttatcactgg ccttggctat tgttggggtt gttttaccct
     3961 tgttgccgac aacgcctttc cttttgttgt ctattgcttg tttctccaga agttccaagc
     4021 gtttcgaaga ctggctttat cataccaagc tctatcaaac atatgtagct gattttcgcg
     4081 agactaagtc tattacgcgt gaacgaaaga aaaaaatcat cgtctctatc tacgtcttga
     4141 tgggaatttc tatttatttt gcacctcttt taccagtcaa aatcggtctg ggtgctttga
     4201 ccatctttat cacctattat ctcttcaagg tcattccaga caaagaatag ttaaaacagt
     4261 agttatttgc cttgataaaa ttgaaagcat attcataaca atatgatata ataaatttga
     4321 agtaatattc aaggagaatc aaatgattta cgaattttgt gctgaaaatg tgactttact
     4381 tgaaaaggcg atgcaggctg gagctcgtcg gattgaactc tgtgataatc tagcagttgg
     4441 tgggacaaca cccagctatg gagtgactaa ggcagcggtt gaactggcag ctaactacga
     4501 tacaaccatc atgaccatga ttcggccacg tggtggtgac tttgtctata atgacctaga
     4561 aattgctatc atgctagaag acattcgttt gactgctcag gctggaagtc aaggggttgt
     4621 atttggagct ttaactgctg ataaaaagtt ggataagcct aatctggaaa agttaattgc
     4681 tgcatcaaaa ggaatggaaa ttgtctttca catggccttt gatgaactaa gtgatgaaga
     4741 tcaaccggaa gctattgact ggctcagtca agccggtgtc actcgtatcc taactcgtgc
     4801 tggtgtgtct ggcgactcct tagaaaaacg ttttgttcac tatcacagaa ttttggagta
     4861 cgctaaaggt aaaattgaaa ttctaccagg tggggggatt gaccttgaaa accgtcaaac
     4921 ctttatcgac caggtggggg taacacaatt gcatggtact aaggttgttt tttaaaaaat
     4981 agaaaggaac tgctagcttt gggtagcagt tttcacttat gtttgaaatt tttaaatcct
     5041 atcaatttaa tcaagaaaag gctcatgatt atggttttat agaaaatagc gaagtctgga
     5101 catatagttg ccagattttg caaggtgact ttgtcatgac tgtgtccatc actgctgata
     5161 atgtgaactt tcaagtcttt gaccaagaga ctggtgacct ctatcctcac gtttatatgg
     5221 aaagcatgag gggaagtttt gtcggaaatg tccgtgaggc ttgtctggag attctttacc
     5281 agattcggaa ggcttgtttt gatgtgcaag attttatctg tcatcagact aagcgtatca
     5341 tgactcaagt tcaggaaaag tatggaaacc agttggagta tctgtgggaa aaatcgcctg
     5401 atacagctgt attgcgccat gaaggcaatc aaaagtggta tgccgtcttg atgaaaatct
     5461 cttggaataa gctggaaaag ggcagagaag gacaagtgga agcagtcaac ctcaagcatg
     5521 accaagtagc taatttgctt tcacaaaagg ggatttatcc agccttccat atgagcaagc
     5581 gctactggat tagtgtgtcc cttgatgata ctttatcaga tgaagaagta ctggaattga
     5641 tagaaaaaag ttggaactta acctctaaaa aatgaaatat tttaataatt ttcatgaact
     5701 ttcaattggc taaatattct ttactgaaga aattttcaga aaatatagga tttaccacac
     5761 cagaggaatg tggtgccatc ttcaaatacc tgattgagaa tgtcaagacg gatcgtcaga
     5821 tcatctattc gcctcactgt catgatgacc tcggaatggc agtggcaaat agccttgctg
     5881 ctgtcaagaa tggtgcagga cgtgttgaag ggactatcaa tggtattagg gagcgagctg
     5941 aaaatgctgc tttggaagaa attgcagtgg ctctcaatat tcgccaagat tactaccaag
     6001 tagaaaccag tattgtccta aatgagacca tcaatacgtc agaaatggtt tctcgcttct
     6061 ctggtattcc agttcctaaa aacaaagccg tcgttggtgg caataccttc tcccacgaat
     6121 ctggtattca ccaagatgga gtccttaaaa atcctctcac ttatgagatc atcacacctg
     6181 aattggttgg tgttaagatt ccgcttggaa aattatctgg tcgccatgct tttgttgaga
     6241 aactgagaga attggcccta gattttacag aagaggatat caaaccactc tttgctaagt
     6301 tcaaggcact ggccgataag aagtaagaaa tcacagatgc agatactcga gctttggtag
     6361 ctggaaccat ggttgaaaat ccagaaggct tccactttga tgatttacaa cttcaaactc
     6421 atgcagataa tgacattgaa gcgctcgtta gcctagccaa tatggatggt gagaaagtcg
     6481 aatttaatgc gacagggcaa ggttccgttg aagcaatctt taacgctatc gataagttct
     6541 ttaaccaatc tgttcgtttg gtgtcctaca ctatcaatgc ggtaacagat ggaatcgatg
     6601 cccaggatcg ggttttggtc actgttgaaa acagagatac agaaaccatc tttaatgcag
     6661 cagggcttga ttttgatgtg ttgaaggctt ctgctattgc ctatataaac gctaatacct
     6721 ttgttcaaaa agagaatgca ggtgagatgg gacgcagtgt ttcttaccac gatatgccta
     6781 gtgtgtaaag gagaaggcta tggcaaagaa aatagtagct ctagtaggag acggaattgg
     6841 cccagaaatc atggaggctg gtttagaagt tctggaggct ctagctgaaa aaacaggttt
     6901 tgactatgag attgacagac gaccgttcgg aggtgcagat attgatgcag caggacctcc
     6961 cttacctgat gaaaccctta aggcaagtag ggaagcagat gctatcctac tagcagctat
     7021 cggtagtcct cagtatgatg gagcagcggt tcgccctgaa caaggcctga tggctctccg
     7081 taaggaactc aatctttacg ctaatattcg tcctgtaaaa atctttgaca gtctcaagta
     7141 tttgtcacca ctcaaaccgg aacgaatttc tggtgtagac tttgtcgtgg tgcgtgaatt
     7201 gacaggcgag atttactttg gagatcatat tcttgaagag cgcaaagcgc gtgatatcaa
     7261 cgactatagc tatgaggaag tggagtggat tattcgcaaa gcctttgaaa ttgcaagaaa
     7321 tcgcagaaaa atccttacta gtatcgataa gcaaaatgtt ctagcgacct caaaactctg
     7381 gcggaaagta gctgaggaag tcgcacagga tttctcagat gtaaccttgg aacaccagct
     7441 ggtagactca gctgctatgc ttatgattac caatcctgct aagtttgatg ttattgtaac
     7501 ggagaatctt tttggagata ttttatctga tgaatcaagc gtcttatctg gtacacttgg
     7561 ggttatgcca tcagccagtc attctgaaaa tggaccaagt ctctatgaac ctatttacgg
     7621 ttcagcacct gatattgcag gtcaaggaat tgccaatcct atttccatga ttttatcagt
     7681 tgccatgatg ttgagagata gtttcggacg ttatgaggat gcagagcgta tcaaacgtgc
     7741 tgttgagaca agtctggcgg caggaatttt aacgagagat ataggaggtc aggcttcaac
     7801 aaaggaaatg acggaagcta ttattgcaag gttatgaagt tagacgaaaa aattactcta
     7861 gtccttttga tttggaatgt catcattttc ttgatttatg gcattgacaa attcaaggca
     7921 agaagaagag cttggcgcat ccaagagaaa atcttactca ttttagccct tacttgtggt
     7981 ggttttgggg cctggttagc tggaatcact tttcaccaca agactcgaaa atggtacttt
     8041 aaaacagttt ggtttcttgg gatggtgacc acactagtag ccttatattt tatttggagg
     8101 taatggatgg tagggtcttc gagggaacac gctgcttggg ctctagcgga ctatggtttt
     8161 aaggtcgtga ttgcaggatc tttcggtgac attcattaca ataatgaact caataatggc
     8221 atgttgccaa tcgttcagcc tagagaggtt agagagaaac tagcccagct aaaaccaacc
     8281 gaccaggtaa ctgtggactt ggaacaacaa aaaatcatct caccagttga agaattcacc
     8341 ttcgagatag atagcaagtg gaaacataaa ctcctaaata gtttggatga tatcggtatt
     8401 accttgcagt atgaagagtt gattgctgct tatgaaaaac aacgaccagc ctactggcag
     8461 gattagaaaa aatagaaaag gaaatatagt aaactgaaat aagatgtaaa caaatgaatt
     8521 ggtgcttaac atccatttcc agcaattttt tagaaactac agtggactat tctggattca
     8581 acacattata aaattatgac aaaacacatt cacaagaagg ctacgacatt ttaaaaggtg
     8641 agggcggatg tatcgtttgc cctactaaag ttggttacat tatcatgacc agtgataagg
     8701 caggacttga gcgtaagttc gcagccaaag aacgtaagcg taacaaacca ggtgttgttc
     8761 tctgcggtag catggatgaa ctttgcgctt tagcgcaact caacccagaa attgaagcat
     8821 tctactaaaa acattgggat gaagatattc ttcttggttg tatccttcct tggaaaccag
     8881 aagcctttga aaaactcaaa gcatacgggg atggccgtga agaacttatg actgatgtac
     8941 gtggtactag ctgttttgtt atcaagtttg gaaaagcagg tgaacaattg gctgccaagc
     9001 tttgggaaga aggtaaaatg gtctacgcct catatgcttc aatgacaaaa cgattgaaac
     9061 tcgctatgag agcaaggtgt aatggtgtct atggtcgata aggacggcaa actcatccca
     9121 gaacaaggag gagcacgttc aacttcacca gcaccagttg tgatccgtaa agggcttgac
     9181 attgataaaa tcatgatgca cctgtcagat acttttaact catgggacta ccgtcaggtt
     9241 gagtattatt aggatagaga agaagtctag tgttatgaga tattaaagct cctaacactg
     9301 ggcttttgtt tagaatttct tttctttttc tataggatat ggt
//
LOCUS       DAVLVZ010000072         9264 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000072 DAVLVZ010000000
VERSION     DAVLVZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.072"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(91..450)
                     /locus_tag="VYN77_001746"
     CDS             complement(91..450)
                     /locus_tag="VYN77_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1559172.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPIIAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
     gene            complement(749..1663)
                     /locus_tag="VYN77_001747"
     CDS             complement(749..1663)
                     /locus_tag="VYN77_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1559173.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LQDSPGTNNFSLPNVSPTYGQYYTNQSEVIIGKNDLVKVKNTLQYPYSTSAYVESEFR
                     GVKNGKDVTYRGSANFIKDNVLITAAHNIYKHEFGKDAEELYVTPARTPNSVPFGRIK
                     VKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLTGITVTITGY
                     PSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASNRVVGVHTLGDGAN
                     QINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(1813..1908)
                     /locus_tag="VYN77_001748"
                     /pseudo
     CDS             complement(1813..1908)
                     /locus_tag="VYN77_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1985..2296)
                     /locus_tag="VYN77_001749"
     CDS             complement(1985..2296)
                     /locus_tag="VYN77_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002876249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1559174.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(2221..2463)
                     /locus_tag="VYN77_001750"
     CDS             complement(2221..2463)
                     /locus_tag="VYN77_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1559175.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIYQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(2464..3096)
                     /locus_tag="VYN77_001751"
     CDS             complement(2464..3096)
                     /locus_tag="VYN77_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1559176.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(3110..4105)
                     /locus_tag="VYN77_001752"
     CDS             complement(3110..4105)
                     /locus_tag="VYN77_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1559177.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(4102..4800)
                     /locus_tag="VYN77_001753"
     CDS             complement(4102..4800)
                     /locus_tag="VYN77_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1559178.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KIVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(4878..5888)
                     /locus_tag="VYN77_001754"
     CDS             complement(4878..5888)
                     /locus_tag="VYN77_001754"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1559179.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SSQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(5872..6879)
                     /locus_tag="VYN77_001755"
     CDS             complement(5872..6879)
                     /locus_tag="VYN77_001755"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1559180.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIVFSDSY
                     RIYSDMFDFAVDLTPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     VYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVVSKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(6866..7819)
                     /gene="mvaD"
                     /locus_tag="VYN77_001756"
     CDS             complement(6866..7819)
                     /gene="mvaD"
                     /locus_tag="VYN77_001756"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1559181.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEVVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(7801..8679)
                     /gene="mvk"
                     /locus_tag="VYN77_001757"
     CDS             complement(7801..8679)
                     /gene="mvk"
                     /locus_tag="VYN77_001757"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1559182.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(8801..8920)
                     /locus_tag="VYN77_001758"
     CDS             complement(8801..8920)
                     /locus_tag="VYN77_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein C"
                     /protein_id="HEV1559183.1"
                     /translation="MTTGWFQVNGRWYYAYSSGALAVNTTVDGYFNYNGEWVQ"
BASE COUNT         2758 a         2128 c         1616 g         2762 t
ORIGIN      
        1 ctaactaaaa cactcataaa gtatagacaa ttttagacta ctttggatat cttttggtta
       61 gtcattttcc aaaactaatt tatctaattt ttaagttata tttgacttca tcttctcagt
      121 aacatgagta taaacactta aaatctttgt ttcattagta taatctacac gatccattat
      181 cgctattata ggtacttcca attcagcaag caaacttata tgcgaatatc taaaaacatg
      241 agcagtcaat acaaaatcat ctttcttaat ttcacttttc ctaaacctgt ttttcaaaaa
      301 gtaattgaaa caagttatca ttaagggaga accattattt gtaaatatat aatcaccttc
      361 ttctaaaaga agatattctt ttaaaatttc taacactcta tcactaatac caattactct
      421 atatgaagct aaacttttcg gactatccaa aaacacttcg ttattggacg attacattat
      481 agtaaaacga aataagaaca ggacaaatcg atcaggacag tcaaatcaat ttctaacaat
      541 gttttagaag tagaggtgta ctattatagt ttcaatctac tatacaagac gaaattgaag
      601 aaatctttcc attataaaac gcatattatc aaggttttta aacacctaat aatatgcgtt
      661 ttttcctatt ttaaagactt ttatccacct attatacaaa gttaagtatc catcacagtt
      721 tacaattagc cttcattacc taacccattc accatccttc aagagagtaa cctttaagaa
      781 ccgaataaat aaatggcaaa tttcgttcat ttaatttaac tgcactgtta atttgattag
      841 ctccatctcc taaagtatgc actcctacta cgcggttact agcatcataa actgtagatc
      901 cactagaccc ctctaaagta tcaacttggt agtccaagaa catgccatca tcacttaaaa
      961 cttgtttttt atctgtatac atttgatgaa ttttaaaatt atatgatgga tagcctgtga
     1021 tagtcacagt tattcctgtc aaattttttt gactagtagg aagacccaaa gtccctaatt
     1081 ttgcaccaat gggctcttct agaattaata aagccaagtc atattccctt gcatccttag
     1141 aatttaaatt tctaaattcc ttcaaataac gaacttcttt tacttttatc cgaccaaacg
     1201 gcacactatt tggagtacga gcaggagtaa catatagttc ttctgcatct ttaccaaact
     1261 catgtttata aatattatga gcagcagtaa taaggacatt atcctttata aaattggcac
     1321 tccctcgata agttacatct ttaccatttt tcacaccacg aaattctgac tctacataag
     1381 ctgatgtaga ataaggatat tgtaaagtat tttttacctt aactaaatca tttttaccaa
     1441 taatgacttc tgactgattt gtataatatt gaccataagt cggtgaaaca ttgggaagtg
     1501 aaaaattatt agttcctgga ctatcttgta atgtctcact tacctcatat tcttcacctg
     1561 tactagaaat aacactcaaa gcagatactg tcgataactg gctagccaat aaagtactcg
     1621 caataattga aatacccaat tttttataaa cagttttctt cattattgta tcctcctaat
     1681 gtaattatag cgtactattc taaatttctt aatctactat agaatcaaga aatctaccac
     1741 cttctttaaa taccctccat tatcacataa acaggtaaac ttttcaatta atgactgcgc
     1801 ttttcaatca cgctagaggt acttacttgc ttctttgata ctaagttcag ccattctttc
     1861 cttgtttttc tcaataaagc atgttaccca agtgggattc gttctggtgg tcaatagcga
     1921 cacgtctcag tcagatatta tctgataggc tccattttat actcaatgaa aatcaaagag
     1981 caaactagga agctagccgc agattgctca aaacactgtt ttgaggttgc agatagagct
     2041 gacgtggttt gaagagattt tcgaagagta ttaagattat ttcttctagt tcagggtgtt
     2101 catacaccaa actccctact actcgatcta ggatatctac cgtgtcccac caggattttg
     2161 tcacgactaa ctgctctagc ttaggcaaat cggtttcctt tagataagac tgcattgctt
     2221 tcaaatagtt agcagccaca tattggtatt ttctaggctc cttttcccag caagtgtctg
     2281 caaaatccca atcgataatc tttgtttttt tcgcttctgg taaatatttt tatagagttt
     2341 atttctttca ggcaccgcaa tacctagaaa agaaaattga tggcgcatat aggcttccat
     2401 ggaccttgct tttttagagt cttttgctgc ttctagctcc tcaagtaaat ctgctaaact
     2461 catctaaaac tcctcatgcc cgaccaaatg gtgctgaaag gcatagacag ccgcctgggt
     2521 acgatcgcta acttcaagtt tggcaagaat gttggacacg tgggtcttga ccgtcttgag
     2581 agagataaag aggtcatctg cgatgcgctg attttcgtag cccttggcga tgagttggag
     2641 aacatctcgc tcacgcgcag tcaattcttc atgaagttcc atatgattgc ggtggtattc
     2701 aaccttcttg ctaacctctt gctcaatggc cagctcgcca gcagctacct tactgacggc
     2761 atgaagcaat tcatctgcac tagaagtctt gagcatatag cctttggcac cagcatctaa
     2821 gactggcatg attttttcat tgtccaaata agaggtcaca atcaaaatct tggcttcagg
     2881 ccattcttta aggattgcta aggtcgcgtc aatcccattc atctcaggca tgacaatatc
     2941 catgacaatg acatctggac gcagttccaa ggccaagtca atcccttgag acccgttgga
     3001 cgcctcaccc acaacttcta catcgtcttg gaggtcaaag tagcttttca aacccaaacg
     3061 gaccatttca tggtcatcta ctagtaaaat tttcatcttt actcctttat cattccttat
     3121 ctaacagggg aatacggata tcaaccgcca gcccttgctt gggagctgtc aagagttgaa
     3181 ccgtccctgc catatcttca acccgctcct tgatatttcg cagtccataa ctcaagtcgt
     3241 ctaagctccc taactggaaa ccaatcccat tgtccaccac cttcagttgc aattcaacat
     3301 ctgtctgata gaggtagaca tctaggcaag atgcctgggc atggcggagg gtattgctaa
     3361 tcaactcttg caggatacgg aagatatgct cctcgatttt cttaggcaat ttcgtcatat
     3421 tctgcttgag actaacccta agatcactct tgtcctcaag ctcttttaag agaatctgaa
     3481 tcccttctat caagctcttc tgctccagtt caactggtcg caaatgcaag agcaaaaccc
     3541 gcaaatcctt ctgggcagtt tctaaaatag ctgtgacact ctgcaactgg atctgcatct
     3601 tttctctatc caatttcaaa gcctgctgac tgatacccga taaaatcatg tgggccgcaa
     3661 acaactcctg actgactgta tcgtgcaaat cccgagcaat tcgcttccgt tctttctcga
     3721 tgatttcctc ttcctgagca aggctatgat tttcagcttt ttgaagagct tctgtcaaaa
     3781 ggttaagttt acctgataag gacttgaaac tggcatccaa atctggatct gcaacctgaa
     3841 ccacttcttg ccctgccaat aaacgcttga gattagcctg catttttctt agagaaagct
     3901 cttcgatccc tcgccaaaac agggctaaga gacaggttat ggacatgctg aaaaccaaca
     3961 ataaaaagac aaatttttct gttttttcga catcgtgcaa aaagatagac cagtcaaaat
     4021 caagtatttc cagcaagctg tgggagaaaa aaaagacaaa caggaaggag gtgagagcaa
     4081 taatgacata ggcttgtttt ttcatcctct aaccacctcc acatcaccaa tcatagtggt
     4141 caagaaaatc ttgacactct tgttactctt gagatagtct tttgtttctt gatgatagtg
     4201 ttcattgcgg agggctcgct tgggctggtt gaaaaaagtc aaatccccat agagacagtt
     4261 aacgctgaga ctgacttcca catctacagg tacgatgatt ttggtcgttc ctactatctt
     4321 tctgaggata atgacattgt catgattggt taagatgacc ctctccagat gaatagtgtc
     4381 cttgcccatg aagcgaaaga gattgatatc atcgaattgg caagtctggt agcttgaaaa
     4441 atgatgaaga tttccaaacc aacgattttt ctccttctta accgtcacga cctcttcaaa
     4501 aaccaaattg gtctgctctt tttcctggtt catcatcggg taaagaagaa agaggctata
     4561 gataaccgca acaaaaatag ctagaatcac aaaaggattg agcataacga tgaaaaagaa
     4621 gagaatggtt gccgctacta aaagaagatt atttccctct ttaccagtgt agtagcgaat
     4681 caaaagcaaa aagaggaata gtatcagcag aaaacgcgaa aaatgctctg ataccatcaa
     4741 aatcagagct cctgtcagaa gacaggcttc gataaataaa aagattttaa attttctcat
     4801 aggttcatcc tctcccttct attttatcac aatttaaaaa agtcacctca gtctgaggat
     4861 ggaaaaaaag gcgctggtta cgcctttttc atctgatcat ttgcttcttt taattttcca
     4921 taaagaagat agtctacttt ttgtagatct gctatggtgg cacagttaag ggaacacata
     4981 atcaagcgta gatctgcttt ccagccttgg acaatgccaa tcacttcttc aactgtgtag
     5041 gtttcaacca attccagaac ggttcgtgac aatcccacag ccttagcacc aaaaaccaag
     5101 cacttaatca tatccagcgg attccgaacc cctccactaa ccaagagttc gaccttatct
     5161 ttccattctt gggcattgag aagggcctgc atggtagact gaccccattg attgaggtaa
     5221 tcacgctggc tactacgacg gttttcgata taggcaaagc tggtgccacc acgacccgat
     5281 aggtccactg tacgaacacc gaattcatag gctctttcga ttgtcttggc atccattcca
     5341 aagcccactt ccttgaggac aataggaacg ggaatttgct tgctataatc tgctagatgc
     5401 gattgccagc ttctaaactt cctttctccc tcgggcatga gtaattcctg catgacattg
     5461 acatgcactt gcaatagaac aggattcatc tcttctacag tctgaagtcc taactcgaca
     5521 ggcttgtcca atccaatatt ggttccaagg aggagattgg gatgactaga cttgacagaa
     5581 aaagaatcat ccgttggatt tttgagggct gcgctataag aacccgttac aaataaaata
     5641 ccacaggatt ccgccacttg agccagcttt tgattgattt ctcttccctt attacttcca
     5701 ccagtcatgg cattgatata aaaaggaaag tcccactttc gaccagcaaa ctctgtcgaa
     5761 agatcgattt catccagatt gtaaagaggc aaggaagaat gaatcagctc cacctcatca
     5821 aagctattat aggaactttt ctgctcaagg gcatagagga tatgctcgtc cttacgattt
     5881 gtcgtcatgt cctatccttt cttgatataa gagctcaatc cccagatcgg cccaacgatt
     5941 ttttaaggtt ttggttgatt gcgcatcaaa actcagggcg atgccacagt caccaccacc
     6001 agcaccacta ctcttggcaa cggcctgcaa atcttgactg gcttctttca actgtctaag
     6061 caaaggcgtg taaatatctg tactcaagcc ttctaaaagc ttgctgacta cttctacttg
     6121 ctcgataact ttttcggctt tcccctgttc caaggcttcg accaaagaaa ccaccgtttc
     6181 ttttgaggaa cttaaaaaat tttgattgat attttgcttg atttgctgaa ccatgtgact
     6241 cgatacagcc acttccttgg tccatcccac taagaaatca cattctaaag ttggtttcac
     6301 ttgtgaaatt gaaaagcccc aatcacgctc cagaactgtc gccaagtttt cttcttctaa
     6361 ccaagcagcc accttctggc gatcaaatga ctggtagaca accaaatcct ctgccacaat
     6421 acaggcaagg tctcccatgg aaccattgtc tcctcgcttg agcaagacag cgctagtcag
     6481 cttgaacaag agctcctgat caacagaaac atcatacaga gccagtaaag ccttgacaac
     6541 caagacaacg acgctgccac tagaacctag accaaacttt ttcccttctc gttccatttt
     6601 gccacagatt tctagagaaa aaggtcttaa attctgacca cgaacagcga ggaagtctcc
     6661 catcaaagca atcgtttctt gaatcaagct atagtcagga ttgggcgtta agtccactgc
     6721 gaaatcaaac atatctgaat agatacggta gctgtcagaa aaaacaatct cagccctcat
     6781 atagatggga atatccttta tcaaagctaa ctgccctggc tctaaaatag catattcacc
     6841 tgcccaatag agttttccgc aagttttaac agcaatcatc ttgactcaaa tcctttgttt
     6901 ttgacacaat caagcgataa cgatgaccga aaatttctga taaatgctcc aagtctttct
     6961 cctgacagaa gaccttaaca ttgggaccag catccatggt aaagtagcag gcctctcctt
     7021 tctcacgaag ctggcgaaca aagtccatag cctcataaga ggcatccgtc agataagaaa
     7081 aggctggact agcagtcttt gtcgtagcat gcatagccag ggcatttttc tccgttaatt
     7141 ctccaatctt ggcaaaatca ttttccttga gataaatcag catatcctga tagtccttct
     7201 cagactgacg aacccagtcg tcgaaagtcg tcgaggtttc cacacaaagt ttcatcccgt
     7261 cacggctaga gattggtttt ttcttgtcct ctagcaccaa cataatcata gctagtttca
     7321 agtctgtctc tacagggtaa atttctccac tatccttatc ccaggctcct agtggtccat
     7381 aaaaactccg agaagaagag cctgaggcaa atttggcttc ctgtgccaac tgacttctat
     7441 ccaatccaag tttgaaataa gcattacaag ccttgaccag ggcggacaaa ccactagaac
     7501 ttgaggacag gcccgctgcc gtaggcatat tgttttgagt atcgatacgg acaaagccct
     7561 caccagctgg acgataacgg tcaataatct tactcatctt ggcatgctcg acctcatttt
     7621 gtagctgacc attgatgtaa aattcgtcag ctgttacatt ggctggtaaa ggcgacaagg
     7681 tcgtctctgt atacatattt tccaaagtta gagaaatact gctagtagca ggcaccacct
     7741 ctttttcttt tttctttccc caatatttga taatagcaat atttgcgtag gaacgtactg
     7801 ttacaggctc tctatccatg tctgaacagc tcctttctct tctaatcttt ctgctagttc
     7861 ttgtgcgtgt gtcaaattgg ttaccaaggc tatgatacaa cctcctagcc caccaccgct
     7921 catcttggca cccagagcac catggctaag agtcgtttca accaaaaagt ctgcctcagg
     7981 gctactgact ccaatttctt ttaaatgtaa atgcgcttga ctgaggattt gtcccagtcc
     8041 ttcagcatct ttttgtgaaa tcgcaacttc tgcttgctgg gttaattctc ccaaggcatg
     8101 caaaaacggt agggcatcct tgcccttatt ttgaaccact tggatggctt cacgagtatg
     8161 accataaaca cccgtatcgg caatcaccaa ataggcggat aaatccatct caagttctgt
     8221 aaatcctacg ttcttgataa agcgaatagg ttggtcactg agacaggtct tagcatccaa
     8281 accactagga ttcatatggg caatcatttc agctcgattg accaagattt ctagtacatc
     8341 atgaggcaga tcagcctgat agtagtcaaa taccgcacga atggccgcta tgctgatagc
     8401 cgctgacgaa cccatccccc gtttctcagg gatagccgag tcaatctcac aacgaatgca
     8461 ggcttctgtg atatccaaat actccagtga ggcataaacc gccatggaca aggtatcctc
     8521 ctcataaagg cgccaaggac tctctgcagg aactacctta caggtcacct ccacctccaa
     8581 aagaggcagg gaaatggcag gataaccgta aacgaccgca tgttccccta ttaaaattat
     8641 cttactatgt gcctgaccga caccaacttt ttttgtcatt ttttcctttt actagacgaa
     8701 aaaacgtctt atttttcata caagtattaa ttctttccta tctattttat tatattttca
     8761 caaaaaaagc gattgtttcc attcacaatc gcttctttca ttattgaacc cattcgccat
     8821 tatagttgaa atagccatct acggtcgtat tcactgccaa ggcacctgag ctataagcgt
     8881 agtaccatct gccattgacc tggaaccaac ctgtcgtcat agaacgacga aagaaactcc
     8941 ataccattaa gtaaagagga aagtcgtgag ggagcatgcg ccattgacaa cctgttttag
     9001 tgacgtacaa agtctcatta acaagtactc gtttcggcca tttataggtg cggtgtttgg
     9061 agaaataggg ttcaatcttc gcccattctt gatcatttaa atcagtatca tatgctttgc
     9121 gtgtcataac tctagtttaa catttttttg tgaatagagg ttctaaataa tcgaccacga
     9181 aaatttctta agtggaaaac gccttatgaa gtatgctacg ggaaagttat gcacttaatt
     9241 tgacaattca agatgtaaaa atat
//
LOCUS       DAVLVZ010000073         9085 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000073 DAVLVZ010000000
VERSION     DAVLVZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.073"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(383..1231)
                     /gene="dprA"
                     /locus_tag="VYN77_001759"
     CDS             complement(383..1231)
                     /gene="dprA"
                     /locus_tag="VYN77_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1559184.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1327..2016)
                     /locus_tag="VYN77_001760"
     CDS             complement(1327..2016)
                     /locus_tag="VYN77_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1559185.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(2028..2906)
                     /locus_tag="VYN77_001761"
     CDS             complement(2028..2906)
                     /locus_tag="VYN77_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1559186.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2896..3765)
                     /gene="licA"
                     /locus_tag="VYN77_001762"
     CDS             complement(2896..3765)
                     /gene="licA"
                     /locus_tag="VYN77_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1559187.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKGEQGADFGYYGVNRYQRA
                     VKGLASYGGTDEK"
     gene            complement(3782..4804)
                     /locus_tag="VYN77_001763"
     CDS             complement(3782..4804)
                     /locus_tag="VYN77_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1559188.1"
                     /translation="MINQIYQLTKPKFINVKYQEKAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLL
                     LVGSSRSGRIDFENAIQMMKVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4809..5516)
                     /locus_tag="VYN77_001764"
     CDS             complement(4809..5516)
                     /locus_tag="VYN77_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1559189.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVLHAEDLVDKYLPLHKERIIITKGGADRNTSIENIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIKLAQNHDAVDTVVEAVDTIVESTNGQFITGIPN
                     RVHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5891..7378
                     /gene="tacF"
                     /locus_tag="VYN77_001765"
     CDS             5891..7378
                     /gene="tacF"
                     /locus_tag="VYN77_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1559190.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYSYGLRAISNVKDSKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7388..8191
                     /locus_tag="VYN77_001766"
     CDS             7388..8191
                     /locus_tag="VYN77_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1559191.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYVSGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLTFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8193..9002
                     /locus_tag="VYN77_001767"
     CDS             8193..9002
                     /locus_tag="VYN77_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1559192.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2843 a         1833 c         1535 g         2874 t
ORIGIN      
        1 tataaattaa tttgactttc ctgatagagt tgttcacatc ttatttcaat tcactatact
       61 ttcccttata ctcaatgaaa atcaaagcgc aaactaggaa gctagccaca caggttgctc
      121 aaagcactgc tttgaggttg tagataagac tgacgaagtc agctcaaaat actgttttga
      181 ggttgcagat ggaagctgac gtagtttgaa gagattttcg aagagtatta tatgagaaaa
      241 atcctctact ctactaatcc acttagaaaa aacgcatatt atcaagtgtt aaaaacttgc
      301 taatatgcgt tttattatgg aaatgttggg ttcattttct cttaaaattg attttttctt
      361 agaattctag cttaggtcat ttttaaaatt caaattccgc aagaacatct tgcccactgg
      421 tgaccaattt tgctccttct tgaatcaaat gatggcaacc gtctgatagt ccatctaaaa
      481 tgctaccagg aatagcaaag acatcgcgtc cttcttccat tgctcgctca cacgtaatga
      541 gactacctga acgcatctta gcctctgcta caatcacacc acgacaaagt ccagcaatga
      601 tgcgattacg ggcaggaaaa tgaaatttca gaggttgttc gccaggtcca tattcactta
      661 gaaccagatg gtcattgccg atgtagtctt gcaagcgttt attggcttta ggataaaaca
      721 catccagtcc tgttccaatc actgcaatgg tttttccgcc attctgaaga gctgccatat
      781 gagctgctgt gtcaatgccc ttggccagac cactgacaat aaccagttca ttttccaagc
      841 cttgaatgac tttttcaact gacttagctc cctgtttgct acaagcacga ctgcccacga
      901 ccgctacctt cgggaatttc aggaggtcaa gatttccctt gtaaaataaa agtacaggcg
      961 catcatatat ttcactcaaa tcccaaggat aacagtcatc taaaatagag aaagatggaa
     1021 atttttgaaa ctctttcgac aaatgcgcat cgtctatctg aaaataacgt tccataaaaa
     1081 cggctggatt acggcaacct gatatatctg caatatcacc caacaaaagc tcctgatcaa
     1141 cattttcacc gtattctagc actttcaaaa tctgttgatt ggtcaaacct gattttttta
     1201 acttatagat ttcatagttt gtgattttca taaataactc catttctttt tctactcatc
     1261 tatctattcg taaaaattcg taaaaaatca aaaaacatcc gactattttg tcagatgttg
     1321 gaatctttaa ttttcgtttt taagaatttc ttctaattta cgatagtctt ggacactatc
     1381 gatctcataa atgctattgc cttctaattc ttcaacatag acatctagct ctttgatatt
     1441 atccttaacc atattgtccc aatagagatc aacaaattca ccacttgcat aagccttgtc
     1501 gataaagctg acaatctttt ctgcagttgg agcatcccag aaggatacac cactaaggat
     1561 gcgacctgcc ttgctatcaa caataatgtc ttgaaccttg tagtcatctc catagaccaa
     1621 gaaccattcg ttggtacaat cttcacgata aacactaaaa taagtcgaac gtgtcaaatc
     1681 attgcggaac atatttttaa agagataatt gtcagcatca ataacatagc tgttggccaa
     1741 ttcttctttt acaagataga gagagtaaaa gttattgtag tcagcgtatt tatcattgaa
     1801 aacgaggcga acaccgtatt tttcttttaa gtaatcgaat tgttctttaa gataaccaac
     1861 aatgatgatg atgtcattga ttcctttttc tttgagaaac tcaatttggt attcaatcaa
     1921 aggtttttga ttaacctgaa ccaaggcttt aggggtattt tcagtcatag gacgcaagcg
     1981 agttcccaat ccagctgcta agataatggc tttcacacga atctccttta ttctttaata
     2041 ataatataaa ctccagcaat gacgacaagt gacgtcataa tggtcagcat atctagcggt
     2101 gcacccaaaa aaacaactgc aaacaagacc gtccatacta catagctcac gttcaagcct
     2161 gtagccttgg ctggttgcaa gcgattgata gcgatataat aagccaagta ggaaatcata
     2221 tcaaaggctg caaaaacaat catgagacct agcaattgtc cattggctac tgcagtaaat
     2281 gactgatgag agaagagcac aatcacaaga taggacaaga acgaagtcac ttgacggatt
     2341 aagagggctt cgatttcact cagttcactt tccatagcaa aggagctaag aacactctca
     2401 cttccccatg caatagcaca aaccaaagca cagagaatcc caatgtagaa ggaattaacc
     2461 tgttcaacct tataggtctg ggcaataatc cctccaataa tcaagacaat cccaaataca
     2521 gtatttttcg aaatcttgtg cttcaaaaag aagaaagcca atagaactga aatcgcaggg
     2581 taaatagccg atacagatga agctaaagaa cttccgatat acttaactgc ataaagattg
     2641 gcctgcatac cgatagggcc tgctagcaag gctccgatga taacactgac attgcgaata
     2701 tttaagaaaa ttgagaggcg aactttccct tcttttacca agagaaaagc tagtaagata
     2761 aagatgctca aaaaatcatg agctgcagcc accacaaagg gtgacaaatc tgtaaaaatc
     2821 gaaaagatat aagcactaac cgttagaccc aagccccaga aaatacctga gagaaggcca
     2881 aaaggaactc cgtttttact tttcatctgt tcctccataa gaagccaaac ctttaacagc
     2941 tctttggtaa cgattcacac catagtaacc aaaatctgca ccttgctctc ccttatagac
     3001 agtccataga ctccaaatag tatcttgtaa aattttataa atagcaatct tttcatgaga
     3061 aaccggtgtt tggtcactct catagtgaga taagaaagtt tcttcctctt gggaagtgaa
     3121 ttcagactct aaaaagaggg cagccaaatc ccacattgga tcattcattg atgaatattc
     3181 ccagtcaatc aaataaagtc gtccttgagg tgattcgata aagttttcag gcaccaaatc
     3241 gatatgacaa gattttctgt caacacctaa gtcagccagt cttttctcta aggagaagac
     3301 tgcatttcta acagattcat agttggcata aggaatttgt tcttcaatca aggattcgta
     3361 ttttttgatt tcttcaaaag gagcaaattc tcctcttaat tccttagcag atgtatgaat
     3421 agtttgtaat attggagcaa ttttgtcgaa cttggtcttg attgacgttg aatcaagcgt
     3481 aatcgcagat tcgatatact catttacttt gatgccagct tcaatatcaa aaagataatt
     3541 ttttacatct aagcctaaat cctttagtag ttcaagattg tacttttcat cttgtcgatt
     3601 gataagcttt tctgtccctt taccaaagaa tttaacaatg tattgcttat ttgttgtttt
     3661 ggccaaatag ttttgattgg tcattccacc cagttgttca acactgagga cttcttcttc
     3721 ttgactaagt aaggaagaaa ttttttcttt aatgattttc tccacaatta acctccagga
     3781 cttatacttc ccacttaaac actgttttaa aggctgtatt taaatcggtt gcaaagacac
     3841 ggtgaatatc tttaatttct cttacaggtt cttctagata aaggatattt ttaagacgat
     3901 tggcaaattt cttgactttc atcatttgga tagcattttc aaaatcaatg cgaccagaac
     3961 gagatgaccc aaccaagagc aagccctttt ctaaggcatc gcgagtattg agattgactt
     4021 tatattcgct aactcccatc atgagaattg ttccctgagg acgaatgtag cgaatcaagt
     4081 cattaatagc tggtccagta ccatcaccac cacaacattc aaaagcatgg tcaaaggcca
     4141 actcttcagg aatattatcc gtaatatagc attctttggc aaatgagaag agttccaact
     4201 tttcccaatg acgaccaata accacaatct ctgcttctgg caaagtataa ttgataatat
     4261 tggcaaccac aaaagctaaa cttccatctc caataacggc gatccgctcc cgcttgctat
     4321 gagcaagagt caatagacga ttcatagcgt gcatgcccac actgacaaac tctgtaatgg
     4381 ctgcaaccgt atcttcaata gcatcataag ccaccacacg atctttaggg agagaaacaa
     4441 actctctcat aaagccatca aatccactag acaagaaatg ggtccctgtc atgtagtttt
     4501 catagaattc ttcatcactc tgcataggag actgattggg aatcatgaca actttttgac
     4561 caacctcgta ggttcctgtc gggtcagaaa tgacggttcc acatgactcg tgaatcattg
     4621 ccattggaag ctttttattc aaaatcttgg gatcacgttt tccctggtag taacgctgat
     4681 ccgcatgaca gacagccatg tagttgggac ggataaggat atgattctct tggtcaatag
     4741 ccttttcctg atatttgaca ttgataaact taggtttagt tagttgataa atttgattaa
     4801 tcattttact agtctttctc aatcatactt tttgcaatct tcaaatctgt tacggttgta
     4861 atcttcagat ttgagtattc gcctttggcc aaggctacat cttttccttt gatcacaaag
     4921 attttacatg catctgtcaa gatttccttc tcttcatcag aaagagatcc ataaaggtcc
     4981 atgaagtcct tgcaacggaa tgtttgaggt gtttgtccct gatagaggtg aacacgattt
     5041 ggaatacctg taatgaattg accattggta ctttcaacga tagtatccac tgcttctact
     5101 actgtatcca ctgcgtcatg attttgagca agtttgatac tgtcttgaat catgcgaagc
     5161 gtaataaatg gacgaacaga atcgtgggta acaacgatat cctctggagt aagcggacga
     5221 taagcatcaa tggcttcaat gatgttctca atacttgtat tgcggtcagc accacccttt
     5281 gtaatgataa tacgttcctt atgaagagga agatatttat ctacaagatc ttctgcatgt
     5341 aaaacccagt ctccatgaac cccaactaca attttttcaa tacttggttc caagacaaat
     5401 ttttcaattg tatgaatcaa aataggtcga tcacctagct ctaaaaattg ttttggcaag
     5461 ttactgattc ccatgcgtgt gccagttcca ccggcaagaa ttcctgcata aatcatctat
     5521 tttctccttt gtcttactaa aaaacttatt ctctctatta taccacaatc tagtcctata
     5581 taccacaatc tagtcctata taccacaatc tagtcctatc atgaaagaaa tactggtcct
     5641 ccccttctag aataggagaa ttatgcagtt ctattattca aagaaacaag cctatttcta
     5701 gtaatgacta ggaattctgt agcaattact aataaatgac tgcgaaattt tagagtcctt
     5761 cagaataata tactttcctt aaagaaaact taaaatttaa gatttgaaac ttcaaagcga
     5821 ttgtttgact tgctgccctt catttcttat tagctaactt tatgatataa tgaaaaacga
     5881 ggtaaattga atgaaaagta taaaattaaa tgctctatct tacatgggaa ttcgtgtctt
     5941 gaatattatt tttcccatcc taactggaac ctatgtcgcg cgtgtcttgg accgaactga
     6001 ctatggttac ttcaactcag tcgacactat tttgtcattt ttcttgccct ttgcaactta
     6061 tggtgtctat agctacggtt taagggctat cagtaatgtc aaggatagca aaaaagatct
     6121 taacagaacc ttttctagtc ttttttattt gtgcatcgct tgtacgattt tgaccactgc
     6181 tgtctatatc ctagcctatc ctctcttctt tactgataat ccaatcgtca aaaaggtcta
     6241 ccttgttatg gggattcaac tcattgccca gattttttca atcgaatggg tcaatgaagc
     6301 tctggaaaat tacagttttc tcttttacaa aactgccttc atccgtatcc tgatgctggt
     6361 ctctattttc ttatttgtta aaaatgaaca cgatattgtt gtctatacac ttgtgatgag
     6421 tttatcgacg ctgattaact acctgattag ttatttttgg attaaaagag acatcaaact
     6481 tgttaagatt cacctaagtg attttaaacc actctttctc cctctgacag ccatgttagt
     6541 ctttgccaat gccaatatgc tcttcacttt tttagatcgc ctcttcctcg ttaaaacagg
     6601 gattgatgtc aacgttagtt actataccat agctcagcga attgtgaccg ttatagctgg
     6661 ggttgtaaca ggtgcaattg gagtgagtgt gcctcgtctc agttactatc tggggaaagg
     6721 agacaaagaa gcctatgttt ctctggttaa tagaggtagt cgaatcttta acttctttat
     6781 cattccactg agttttggac tcatggtttt aggaccaaat gccatcctac tttacggtag
     6841 tgaaaaatat atcggaggcg gcatcttgac ctctctcttc gcttttcgta cgattatcct
     6901 ggccttagat accattcttg gttcccaaat tctctttacc aatggctatg aaaaacgtat
     6961 cacagtttat acagtctttg ctgggctact caatttgggc ttgaatagtc tcctcttttt
     7021 caaccatatc gtggctcccg aatactactt actgacaact atgttatcag aggcttctct
     7081 acttgttttc tatatcattt tcatccatag aaaacaactc atccacttgg gacatatctt
     7141 tagctatact gttcgatact ctctcttttc actttccttt gtagcaattt atttcctgat
     7201 taatttcgtg tatcctgtgg atatggtcat taatttgcca tttttgatta atactggttt
     7261 gattgtcttg ctatcagcta tttcttatat tagtctactt gtcttcacaa aagatagcat
     7321 tttctatgaa tttttaaacc atgtcctagc cttaaaaaat aaattcaaaa aatcatagga
     7381 gtttaaaatg aaacaactaa ccattgaaga tgccaaacaa attgaattag aaattttgga
     7441 ttatattgat actctctgta aaaagcacaa tatcaactat attattaact acggtactct
     7501 gattggggcg gttcgacatg agggctttat cccttgggac gacgatattg atctgtccat
     7561 gcctagagaa gactaccaac gatttattaa catttttcaa aaggaaaaaa gcaagtataa
     7621 gctcctatcc ttagaaactg ataagaacta ctttaacaac tttatcaaga taaccgacag
     7681 tacgactaaa attattgata ctcgaaatac aaaaacctat gtgtctggta tctttatcga
     7741 tattttccct atagatcgct ttgatgatcc taaggtcatt gatacttgtt ataaactgga
     7801 aagcttcaaa ctgctgtctt tcagtaaaca taaaaatatt gtctataagg atagcctttt
     7861 aaaagattgg atacgaacag ccttttggtt gctccttcga ccggtttctc ctcgttattt
     7921 tgcaaataaa atcgagaaag aaattcaaaa atatagtcgt gaaaatgggc agtatatggc
     7981 ttttattcct tcaaaattta aggaaaagga agtcttccca agtggtacct ttgataaaac
     8041 aatcgattta acctttgaga atttaagcct tcctgcacct gaaaaatttg atactatttt
     8101 gacacaattt tatggagatt atatgaccct accaccagaa gaaaaacgct tctacagtca
     8161 tgaatttcac gcttataaat tggaggatta ggatgcaata tttagaaaaa aaagaaatta
     8221 aagaaattca actagccctg ctggactata ttgatgagac ttgtaagaaa catgatattc
     8281 cttattttct cagttatgga accatgcttg gagccatccg ccacaaaggt atgattcctt
     8341 gggatgatga tattgatatt tccctttatc gtgaggatta tgagcgttta ctgaagatta
     8401 ttgaagaaga aaatcaccct cgctacaagg ttctttccta cgatacatct tcttggtact
     8461 tccataattt cgcatcgatt ttggacactt ctactgttat agaagaccat gttaagtaca
     8521 agcgtcatga taccagcctt ttcatcgatg tcttcccaat tgatcgattt acagacttga
     8581 gcattgtcga caagagctat aagtatgtgg ctcttcgtca actagcttat atcaaaaaat
     8641 cacgagcagt tcacggtgat agcaaactaa aagattttct tagattatgt agctggtacg
     8701 ctctccgatt tgtcaatcct cgctactttt acaagaaaat tgatcaacta gtcaaaaatg
     8761 ctgtaaccaa cactcctcaa tatgaaggag gagttgggat cggtaaggaa gggatgaaag
     8821 aaatcttccc agttgatacc tttaaagaac tgattttaac tgagtttgag ggccgtatgt
     8881 tgcctgttcc caaaaaatat gaccaatttt taacccagat gtatggcgat tatatgacac
     8941 caccatcaaa agaaatgcaa gagtggtata gtcatagcat taaagcttat cgcaaaaact
     9001 gattgagggg gattatacag actactaaga tagaggttat tcaaaaacat aattttagta
     9061 gaaaatgaaa tacatattcc cacaa
//
LOCUS       DAVLVZ010000074         8792 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000074 DAVLVZ010000000
VERSION     DAVLVZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.074"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            374..3649
                     /gene="rexB"
                     /locus_tag="VYN77_001768"
     CDS             374..3649
                     /gene="rexB"
                     /locus_tag="VYN77_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1559193.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMIFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPCVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3646..7296
                     /gene="addA"
                     /locus_tag="VYN77_001769"
     CDS             3646..7296
                     /gene="addA"
                     /locus_tag="VYN77_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1559194.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAANELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SDSFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLAHVVAISKEKNGRALTNASRKADLKPLADAYNEERKAQ
                     FAKLGQLSDQITILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNIHQLVFANTKLTPNPDNKAEFLLYDKADTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLINAQKMAS
                     SQKGLIHTALSEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     HLQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7511..8689
                     /locus_tag="VYN77_001770"
     CDS             7511..8689
                     /locus_tag="VYN77_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559195.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSIC
                     VCGNVIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKNGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDYRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2708 a         1852 c         1873 g         2359 t
ORIGIN      
        1 ttcaatcatt ctatcaagaa tattgaatat ataaaggcac gtaatattaa aacttcttct
       61 gatatcgaat tagagtccga tgttgatggc gataaatcag acaatctacc cgtcgacatt
      121 aagatactgg gacaacacat tgaagtgtat tccgtaccta agaaacaatc ataaaactca
      181 aagccttgtt accgaggctt ttttattatg tgattttcag tagccagact tattctatct
      241 aatacaagta aagctaagta tttaagattt agcagatgat gaagaaaaga aaggatggcc
      301 taaatatttt aacagattta cgaggctaat catggtataa tatagggtga tgaaaaattc
      361 caacgaggct gagatgaaat tactttatac tgatattcga acttctttga cagaaattct
      421 aacaagagag gcagaagagc tagttgcagc tggcaagcgg gtcttctaca ttgcccccaa
      481 ctctctttct tttgaaaagg aacgcgccgt gctggaatgc ttgtcccagc aggcttcttt
      541 ttcgattacc gtcacgcgct ttgctcaaat ggctcgttat ctggtcttga atgatttacc
      601 agctaaaact actcttgatg atatcggtct tgggttggcc ttttacaaat gccttgccga
      661 actcgatccc aaggacttgc gtgtttatgg cgctattaag caggatcctc aattgatcca
      721 gcagttaatt gagctttacc atgagatgac caaatctcag atgatttttt tggacttgga
      781 gaatttaaca gatgaggata agagggcgga tttactcttg atttttgaga aagtaacagc
      841 ctatcttaat caagggcagt tagcccaggg aagtcagttg tcccatttga ttgaggctat
      901 tgagaatgac aaggtaagta gtgattttaa tcaaatcgcc ttggtcattg acggctttac
      961 tcgtttttct gctgaggaag agcgggttgt ggacttactt cacggcaaag gtgctgagat
     1021 tgttatcggg gcttatgcta gtaagaaagc ctataccagt ccttttagcg agggcaatct
     1081 ctaccaagcc agcgtaaaat ttctccatca tctggcttct aaataccaaa cgcccgctca
     1141 ggactgttct caaactcatg agaagatgga tagttttgac aaggcctctc gtttgttgga
     1201 gtcttcttat gacttttcag aactcgcttt ggatgtcgat gagaaagacc gtgaaaattt
     1261 acaaatctgg tcttgtttga cgcaaaagga ggagttggag ctagtagccc gtagcattcg
     1321 tcagaaatta catgagaact cagacctgag ctacaagcat tttcgtattc tcttggggga
     1381 tgtagcttct taccagttat ctctcaaaac catttttgac cagtatcaga ttccttttta
     1441 tcttggtaga agcgaagcca tggctcatca tcccttgact cagtttgtcg agtctatttt
     1501 agctttaaaa cgttaccgtt ttcgtcagga ggatttgatt aatcttctta gaactgactt
     1561 gtatactgac ctcagtcagt ctgatattga tgcttttgag caatatatcc gctatcttgg
     1621 tattaatggc ttgccagcct ttcagcaaac cttcaccaaa tcccaccatg gaaaatttaa
     1681 tcttgagcgt ttgaatgtcc tccgcctgag aattttagca cctcttgaaa ccctctttgc
     1741 cagccgaaaa caaaaggctg aaaacctcct acaaaaatgg agtgtctttc taaaagaagg
     1801 agctgtgacc aagcagttac aagatttgac aaccactttg gaagctgtag aacaggaaag
     1861 acaagccgaa gtttggaagg ctttctgcca tgttttagaa caatttgcga ctgtttttgc
     1921 tggttcacag gttagtctgg aagacttcct agccttgctc cattctggaa tgagtttgtc
     1981 ccaataccgt accattccag caacagtgga cactgttctg gtgcagagtt acgatttgat
     2041 tgcaccattg actgctgact ttgtctatgc tattggacta actcaggaca atttaccaaa
     2101 aatttctcaa aacaccagtc ttctgacaga tgaagaaagg caaaatctaa accaagcgac
     2161 cgaagaaggc gttcaattac tgattgccag cagtgaaaat ctcaagaaaa atcgctacac
     2221 tatgctttcc ttggtcaatt ctgctcgtaa gcagttgttc ttgtcggctc caagcctttt
     2281 taacgaaagt gaaagtaagg aatctgccta tcttcaagag ttgatccatt ttggatttag
     2341 gcggagagag aagaggatga atcacaaagg actgtctaag gaggatatgg ggtcctatca
     2401 cagtcttttg tctagtctgg ttgcctatca ccagcagggt gagatgagcg atactgagca
     2461 agatttgact tttgtcaagg ttctgtcgcg tgtcataggt aaaaaactag atctgcaagg
     2521 tctggaaaat ccagccatcc caatcagtcc aagcagcaag accttagcca aggacacctt
     2581 gcaagctctc tatcctgcca aacaggagtt ttacctgtct acgtcgggtt tgacagagtt
     2641 ttatcgcaat gaatacagtt atttcctacg ctacgtttta ggcttgcagg aggaattacg
     2701 tttgcgtcct gatgcccgta gtcacgggaa tttcttgcat cgtatctttg aacgcgcctt
     2761 gcagttgcct aatgaagatt cctttgacca acgtctagaa caagctattc aagaaaccag
     2821 tcaagaacgc gaatttgaag ctatttatca agaaagtttg gaagcccagt ttaccaagga
     2881 agttttgctt gatgttgcac ggacaactgg acatattctc cgacacaatc cagccatcga
     2941 aacaatcaaa gaagaagcaa attttggtgg aaaagaccaa gcctttattc aattagacaa
     3001 tggacgcagt gtctttgtac gaggcaaggt ggaccggatt gaccgtttga aagctaatgg
     3061 agcgatagga gtagtagact acaaatccag tctgactcag ttccagtttc ctcatttctt
     3121 taatgggctc aattctcagt taccaaccta tcttgctgcc ctaaaaagag aaggggagca
     3181 gaactttttc ggcgccatgt acttggaaat ggctgaacct gtccaatctc tgatggcggt
     3241 aaaaagtctg gcaggagcag tggtagaagc cagcaaatct atgaaatacc aagggctctt
     3301 cttggaaaaa gaaagcagtt atttaggcga attttataac aaaaacaagg cgaatcaact
     3361 gacagatgag gaatttcagc tcctactgga ctacaatgcc tatctttaca agaaagctgc
     3421 tgagaagatt ttagcaggcc ggttcgccat caatccttat actgaaaatg gcagaagcat
     3481 tgctccatgc gtccagcaac atcaggctat tacaggcttt gaagccaatt accatctggg
     3541 ccaagcccgt ttcctagaaa agttggacct agctgatggc aagcgtctgg tcggagaaaa
     3601 actcaagcaa gcttggcttg aaaaaataag agaggagttg aatcgatgaa gcttattccc
     3661 tttttaagtg aggaggagat tcaaaaactg caagaagcag aagcaaattc gagcaaggaa
     3721 cagaagaaaa ctgccgagca aatcgaagct atctacactt ctggccagaa tatcctggtc
     3781 tcagcatcgg ctggttctgg aaagaccttt gtcatggcag agcgcattct ggaccaattg
     3841 gcgcgtggtg tcgaaatttc tcaactcttt atctcaacct ttaccgtcaa ggctgcaaat
     3901 gaacttaaag aacgtttaga gaaaaaaatc agcaagaaaa tccaagaaac agatgatgtc
     3961 gacctcaaac aacacttggg acgccagttg gcagacctac caaacgctgc catcggaacc
     4021 atggactctt tcacacaaaa attccttggc aaacatggtt atctgcttga tattgcacct
     4081 aatttccgta ttttacaaaa ccaaagcgag caacttcttc tcgaaaacga agtctttcat
     4141 gaggtctttg aagcgcatta ccaaggtaaa cagaaagaga cctttagtca tttgctgaaa
     4201 aactttgctg ggcgtggcaa ggacgaacgg ggtctgcgcc agcaggtcta taaaatctat
     4261 gacttcctcc aatccaccag caatcctcaa aagtggctga gtgactcttt cctcaaaggc
     4321 tttgagaaag ctgattttac cagtgaaaaa gaaaaactga ctgagcaaat caaacaagcc
     4381 ctttgggatt tggaaagctt tttccgttac catctggata acgatgccaa ggagtttgca
     4441 aaggctgcct atttagaaaa tgttcagtta attctggatg aaattggctc cctaaatcag
     4501 gagtccgata gtcaggctta tcaggcagtg cttgcgcatg ttgtcgccat ctctaaggag
     4561 aaaaacggtc gagctctgac taatgccagc cgtaaggctg atttgaagcc cctggctgat
     4621 gcctacaacg aagagagaaa ggcccagttt gctaaactag gacaattatc agaccagata
     4681 accattctcg actatcaaga acgttatcat ggagacactt ggaaactagc taaaaccttc
     4741 caatctttca tgagcgattt tgtggaggct tatcgtcaga gaaaacgaca ggaaaatgcc
     4801 tttgaattcg ctgatatcag ccattacacc attgagattt tagagaattt cccacaagtt
     4861 cgtgagtctt atcaggagcg cttccatgaa gtcatggtcg atgagtatca ggataccaac
     4921 catattcaag aacgaatgct ggaattgttg tctaatggcc acaatcgctt tatggtggga
     4981 gatatcaagc aatccatcta tcgtttccgt caggcagacc cgcagatttt caatgagaaa
     5041 ttccaacgct atgcgcaaaa tcctcaagaa ggcaagctca ttctcctcaa ggaaaatttc
     5101 cgtagtagtt cagaagtgct gtcagcaacc aatgatgtct ttgaacgtct catggaccaa
     5161 gaggtcggcg aaatcaacta tgataacata caccagcttg tttttgccaa taccaaactg
     5221 actcccaatc cagacaacaa ggccgaattt ctcctctacg acaaggccga tacaggtgag
     5281 gaagaagaga gtcaaacaga aacgaaacta acaggcgaaa tgcgcttagt tatcaaggag
     5341 atccttaaac ttcatcagga aaaaggtgtt gcctttaagg aaattgctct tctgacctct
     5401 agtcgtagtc gtaatgacca aattctactt gccctgtctg agtacgggat tcctgtcaaa
     5461 actgacggag agcaaaacaa ttatctccaa tccctagaag tgcaagtcat gctagacact
     5521 cttcgtgtca ttcacaatcc cctgcaagac tacgccttgg ttgcccttat gaagtctcca
     5581 atgtttggtt ttgatgagga tgagctagca cgtttgtccc ttcagaaagc agaggataaa
     5641 gtccacgaaa atctctatga gaaactgatc aatgcacaaa aaatggcaag tagtcaaaaa
     5701 ggcttgattc acacagctct atctgaaaaa ctaaagcaat tcatggatat cctagcttct
     5761 tggcgcttgt atgccaaaac ccattctctc tatgacttga tttggaagat ttacaacgac
     5821 cgtttttatt atgactatgt tggggctttg ccgaatggtc ctgctaggca ggccaatctc
     5881 tatgccctag cactgcgtgc tgatcaattt gaaaagagca atttcaaagg tttgtcgcgt
     5941 tttattcgta tgattgacca agtcttagaa gcccagcacg atttggcaag cgtggccgtc
     6001 gcaccgccaa aagatgcagt agagctcatg accatccaca agagtaaagg gctggagttt
     6061 ccttacgtct ttatcctcaa tatggatcaa gatttcaaca agcaagactc tatgtcagaa
     6121 gtcattctca gtcgtcagaa tggtcttggt gtcaaatata ttgccaagat ggagacaggg
     6181 gcagtagaag accactatcc taaaaccatc aaactctcca ttcctagtct gacctatagg
     6241 cagaacgaag aggaattaca gctagcaagc tattctgagc agatgcgttt gctgtatgtt
     6301 gctatgacgc gggctgagaa aaagctctat cttgtcggca agggttctcg tgaaaagctg
     6361 gaatccaagg aatacccagc agccaaaaat gggaaactaa atagcaatac tagactgcaa
     6421 gcacggaatt tccaagattg gctttgggct atcagtaaag tgtttactaa ggacaagctc
     6481 aactttagtt atcgttttat tggcgaagat cagttgacca gagaagctat cggagagttg
     6541 gaaaccaaga gtcctctcca agatagctcc caagcagaca atcgtcagtc agataccatc
     6601 aaagaagctc tggaaatgct gaaggaggtg gaagtttata atactcttca ccgcgcagct
     6661 attgaacttc ctagtgttca aaccccaagt caaatcaaga aattctacga accagttatg
     6721 gatatggaag gtgtcgagat tgctggtcaa ggtcagtcag taggcaagaa aatcagcttc
     6781 gatttgccag atttttcaac caaagaaaag gtaactggag ctgagattgg tagtgctact
     6841 cacgaactca tgcagagaat tgacctcagc cagcaactaa cccttgctag cctaacagaa
     6901 acactcaaac accttcaaac tagccaagct gtcagagaca agatcaatct tgataaaatt
     6961 cttgctttct ttgacacagt actcggtcag gaaattcttg ctaataccga ccatctctat
     7021 cgcgagcaac ctttctccat gctcaaacga gaccaaaaga gtcaggaaga ctttgttgtc
     7081 cgtggtatcc ttgatggcta tctgctttac gaaaacaaaa ttgttctgtt cgactacaag
     7141 acagaccgct atgatgaacc aagtcaactc gtagaccgct atcgtggtca gttagctcta
     7201 tacgaagagg ctttatcacg agcctattcg attgaaaata ttgaaaaata cttgatttta
     7261 ctcggtaaag acgaggttca agttgtaaaa gtataactta tactctttga aaatctcttc
     7321 aaaccacgtc agcttcgcct tgctgtatat atggttactg acttcgtcag ttctatccac
     7381 aacctcaaaa cagtgttttg agctgacttc gtcagttcta tctacaacct caaaacagtg
     7441 ttttgagctg actgcggcta gctttctagt ttgctctttg atttttattg agtattagaa
     7501 aggagacctc atgacacttc cagttagaaa atccctgcac gatgcagttt tacaggcttc
     7561 gaaagcggat acttgggaac aagctaccaa ggaatggaat gaagtttcct tgatttttaa
     7621 cggtatcggc cgtagcattt gtgtctgtgg aaatgtcatc aaatacgctt atgaactctt
     7681 taacggtgtc acaggccaac gcctctttcc aattggtagc gactgtgttc gtcattttca
     7741 tcgattgtcc cttgaccagc aattggaaga ggaagaaaaa ctgctcagaa aggttgaaaa
     7801 tctaaccaga aaagctcaga aaaaggaaaa aatcaaggtc aataaaagtg actttgacga
     7861 gcgacttcta aaatggctct gggaaaaagg tgttttcaaa cccaatcgtg gcaatcaatt
     7921 tgcgcctgag agagactacc agcttttcct agaagtcttt cagggaggaa gttggaccaa
     7981 ggcggaacca aagaaaaagg ctcggatgga agaagtcctt gaaaagtgta tcaaaccctt
     8041 tttacttggg aaatcggatg accaactcta ccttgtcaag ctaggcaagg agaaaattga
     8101 ctacgaacaa gaactccgta tccaggcaga gaaagaacgc aagaagaggg ataaaatcgc
     8161 caagcaatat gcagacaatc tggttctagc catgggaccc gcagagcgtg cttatcaaga
     8221 ttactttggc tttacagaga ccctgactca agaagaacgc aagtgggaaa aaattctttt
     8281 tggtaaaaat agagcagaac gggctatcaa ggccaaacaa taccaaaaag agctggaaaa
     8341 agaccaacga attgccagtc aggatccaat tgaaagaaaa cagaaacaga cttggctcct
     8401 caattcctat tttcgtgagc ttcctgaaga aaaagctcga ttttctaggc tcttattaga
     8461 atatcgaaaa aatggtgaag tacccttttc aactgaatac ctgtcagatc atctcatcga
     8521 ctttttctat aaaatgaaag cctttgaatt tgaaatcgca ccagaacaag tccgagattt
     8581 tctaaaaaaa agcctgcagg aagactatcg ctcatcagca caaggaagct ggatagaagg
     8641 aattctcctc aactgcctca aaccattttt agaacgatta gtgatatgag taacacctac
     8701 cgtgtaaatt tcatggtagg tgttattgta cccccaaata atataaaaca aagtatgaaa
     8761 gcgcatctca attttaagac ttttttgtaa at
//
LOCUS       DAVLVZ010000075         8362 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000075 DAVLVZ010000000
VERSION     DAVLVZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.075"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..291
                     /locus_tag="VYN77_001771"
     CDS             <1..291
                     /locus_tag="VYN77_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559196.1"
                     /translation="ILYGTVDNHVLTLIFLIKICVDTTIKFLFIRLFDTSTLMKRRIF
                     FLFALDVIAYLFLGYLLVIQKAGYLFSVLLLFSNFSVPFIKEKEYELFKNSK"
     gene            1424..1927
                     /locus_tag="VYN77_001772"
     CDS             1424..1927
                     /locus_tag="VYN77_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559197.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDFASHTISVIQTGVFILEDFRNILLYNDVIERISLYEFIGDIFYSKLTSYC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTD
                     VKEMKWL"
     gene            2225..4054
                     /gene="pspA"
                     /locus_tag="VYN77_001773"
     CDS             2225..4054
                     /gene="pspA"
                     /locus_tag="VYN77_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEV1559198.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAAEAQKKYEDDQKRTEEKGALEEETSRKVNDATLE
                     VQNAYVEYGEANKKKGKDREKKLADAQKKIDEAQEKLTKAKNEFQSVRAMVVPEPEQL
                     AETKKKAEEAKAEEAVAKEKSDKAAKEVEVAKKRVEAEEAELDKKVAELQNKVADLEK
                     EIADAEKTVADLEKEVAKLEKDVEDFKNSNGEQAEQYLAAAEKDLVAKKAELAEAKIK
                     AATKKAELEPELEKAEAELENLLSTLDPEGKTQDELDKEAAEAELNKKVEALQNQVAE
                     LEEELSKLEDNLKDAETNNVEDYIKEGLEEAIATKKAELEKTQKELDAALNELGPDGD
                     EEETPAPAPQPEKPAPAPAPKPEQPAPAPKPEKTDDQQAEEDYARRSEEEYNRLPQQQ
                     PPKAEKPAPAPKPEQPVPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNG
                     AMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEA
                     SGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV"
     gene            4455..5576
                     /gene="mnmA"
                     /locus_tag="VYN77_001774"
     CDS             4455..5576
                     /gene="mnmA"
                     /locus_tag="VYN77_001774"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1559199.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            5717..6171
                     /locus_tag="VYN77_001775"
                     /pseudo
     CDS             5717..6171
                     /locus_tag="VYN77_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            6181..8094
                     /gene="mnmG"
                     /locus_tag="VYN77_001776"
     CDS             6181..8094
                     /gene="mnmG"
                     /locus_tag="VYN77_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1559200.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELTETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
BASE COUNT         2723 a         1481 c         1872 g         2286 t
ORIGIN      
        1 attctttatg gtacagttga taatcatgta ctgactctta tctttttgat aaaaatttgt
       61 gtagatacta cgataaaatt tttattcatc agattatttg acacaagtac tttaatgaag
      121 aggagaatat tttttctttt cgcattggat gttatagctt acttattttt aggatatctt
      181 ttagtgattc aaaaagcagg ctatttgttt tcggttttac ttcttttttc taatttttca
      241 gttccattta tcaaagaaaa ggaatacgaa ttatttaaaa atagcaagta atagaaatat
      301 ggattaaatc ttagaacagc ttaaaatcag atatgataaa gggaaatagt cttatctagg
      361 taactaagtg ctacaaacac agtcgaggat cacttgttag gattacattt taatcatgct
      421 taataagaat ggagaattcc tatggggatt gtattactct gaagttttga ataccttagt
      481 tctcgtgatt ttgagctatt ctagctttag aatccttcag aacgtaaaat tttaagcagt
      541 caaagactta gaatagtctg aaagcattta ttttatagca attctaggct tagaattgct
      601 catatatatg tatttgagct gctcgatagt taggacagct ctattctgag aaatctgagc
      661 tatcaatgct tcagaatagt attcgccaat ggtttttatt gaagaggtga ctgttttatg
      721 atagtctaaa ataaaattga tttaatagag taaatggaga atcgttacat gaaacatttt
      781 tttgcaggaa ttggtgagat aaaacttgtt ggctacttat tgtatatatg tgtaggacta
      841 gcccctcttt ttcatatata tgtcataggt tcggaactga gttttgtgaa aatagtgtta
      901 tctattttgg gagttatatt tgtaagtatg ttaactattg ctcgaattta tcggactttt
      961 ctataaagaa tagtttggac ttgaactgta tctaaaaaca gaggttggtc gattatcagc
     1021 ctctttcttg tcgttgaaaa gagtgagata tattgacttt tgaaaaaaag atgtcataat
     1081 tgagttggga taaacctaaa agtaatatag tagattgaat ctggaatagt ccgttatgac
     1141 tgttaaaata tttctagaaa ttaatttgac tttcctaatc gatttgttca catcttgttt
     1201 caatctacta tactttctcg ttaaaccgtt cggcaattca tggtgacatg gacacatagt
     1261 agtaagcaca ctatgtggct tggcagagct aaaaactgtt cccttgcgat aagcctaata
     1321 agcacaacat agggagttag agaagccgac tctaatcaat cactttgggc agtagtgaga
     1381 actgccccgt gctttttatt ttgagaaaat atggagtttg tcgttgaaat tacttgattg
     1441 tattttggat tatcaagaaa gattcaatgg aaaaacatgt caagtatcaa cgaattataa
     1501 gtatttagag actttcaaag tgaatttttg cttgactgat ttacatcatt tatttggctt
     1561 acacaaaatc acacgagatt ttgctagtca tacgatatct gttattcaaa ctggtgtttt
     1621 tattttggaa gattttagaa atatcctatt gtacaatgat gtaatcgaaa ggatatcttt
     1681 atatgagttt atcggcgata tcttctattc taagctaaca agttattgca tcgtagctaa
     1741 ggatttatct aaaaatacta tgaaattgga cgtcatattt tttgaggata aaaataaaag
     1801 atccgcagtt ttaggtttac gaagagataa aagcggagta tttaaaccag ttactctaca
     1861 ttttacaagc gctaagaaat atgctaaagt tcgtaaaaca gatgtgaaag aaatgaaatg
     1921 gctataaaga ttgtccgcag gcttaagctt gcgttttttg ttgtttaacg aaattaaaaa
     1981 acaagctctc tcatcggaaa tgtttttata ggaatgaagg aagatgatgc tatgtttaaa
     2041 aagagaatcg aaaagggcaa aagtagtgtc tttatctttt tggagtagaa gcttatgata
     2101 tagaaatttg taataaaaat gtaatataaa acatttgaca aatatttacg gggggagtat
     2161 acttaatata gttatagtct aaaaatgact atcagaaaag aggtaaataa agaggtaaat
     2221 ttagatgaat aagaaaaaaa tgattttaac aagtctagcc agcgtcgcta tcttaggggc
     2281 tggttttgtt acgtctcagc ctacttttgt aagagcagaa gaagctccac aagttgtcga
     2341 aaaatcttca ttagagaaga aatatgagga agcaaaagca aaagctgata ctgccaagaa
     2401 agattacgaa acggctaaaa agaaagcagc agaagctcag aaaaagtatg aagatgatca
     2461 gaagagaact gaggagaaag gcgcgctaga agaagaaact tctaggaagg taaacgatgc
     2521 tactttagag gtacaaaacg catatgtaga atatggagaa gctaataaga aaaaaggtaa
     2581 ggaccgtgag aaaaaattag cagatgcgca aaaaaaaata gatgaagcgc aggaaaaact
     2641 aaccaaagct aaaaatgagt ttcaatctgt tcgagcaatg gtagttcctg agccagagca
     2701 gttggctgag actaagaaaa aagcagaaga agctaaagca gaagaggcag tggctaagga
     2761 aaaatctgat aaggcagcta aagaggtaga agtagcgaaa aaaagagtag aggcagaaga
     2821 agctgagttg gataaaaaag ttgctgaact tcaaaataaa gttgctgatt tagaaaaaga
     2881 aatagctgat gctgaaaaaa cggtagcaga tttagaaaaa gaagttgcta aacttgaaaa
     2941 agatgtagaa gatttcaaaa actcaaacgg tgagcaagct gaacaatacc ttgctgcagc
     3001 tgaaaaagac ttagttgcta aaaaagctga attagcagaa gctaaaatta aagctgctac
     3061 taaaaaagct gaattagaac ctgaattaga aaaagcagaa gctgaacttg aaaacctatt
     3121 atctacacta gaccctgaag gtaagactca ggatgaatta gataaagaag ctgctgaagc
     3181 tgagttgaat aaaaaagttg aagctcttca aaaccaagtt gctgaattag aagaagaact
     3241 ttcaaaactt gaagataatc ttaaagatgc tgaaacaaac aacgttgaag actacattaa
     3301 agaaggttta gaagaagcta tcgcgactaa aaaagctgaa ttggaaaaaa ctcaaaaaga
     3361 attagatgca gctcttaatg agttaggccc tgatggagat gaagaagaga ctccagcgcc
     3421 ggctcctcaa ccagaaaaac cagctccagc tccagcccca aaaccagagc aaccagctcc
     3481 agccccaaaa ccagaaaaaa cagatgatca acaagctgaa gaagactatg ctcgtagatc
     3541 agaagaagaa tataaccgct tgccccaaca gcaaccgcca aaagcagaaa aaccagctcc
     3601 agcaccaaaa ccagagcaac cagttcctgc accaaaaaca ggttggaaac aagaaaacgg
     3661 tatgtggtac ttctacaata ctgatggttc aatggctaca ggttggctcc aaaacaacgg
     3721 ttcatggtac tacctcaaca gcaatggcgc tatggctaca ggttggctcc aatacaacgg
     3781 ttcatggtac tacctaaacg ctaacggtgc tatggctaca ggttgggcta aagtcaacgg
     3841 ttcatggtac tacctcaacg ctaacggttc aatggcaaca ggttgggtga aagatggaga
     3901 tacctggtac tatcttgaag catcaggtgc tatgaaagca agccaatggt tcaaagtatc
     3961 agataaatgg tactatgtca atggtttagg tgcccttgca gtcaacacaa ctgtagatgg
     4021 ctataaagtc aatgccaatg gtgaatgggt ttaagccgat taaattaaat catgttaaga
     4081 acatttgaca ttttaatttt gaaacaaaga taaggttcga ttgaatagat ttatgttcgt
     4141 attctttagg tacctatctt atgatttcag gaaatgtcat taaaaaaacg actccttttc
     4201 tctaacttga aaaatagatt agagaaaatg ggttgtttta tttattatag ttatttgaat
     4261 gaagataaga agaaggtata ctcacatcat tcacataatc tgtatgttga ctataagttt
     4321 taaaaaacaa tttttaagct cttccttgtc ttctccaacc aagcgtgtta taatgaatac
     4381 tgctcaagcg accttcaatc gtgaagcaca cacgaccttc aatcgtgaat aaacgaatag
     4441 atgggagact taccatgagt gataactcta aaacacgtgt tgtcgtgggg atgagtggtg
     4501 gtgttgattc gtcggtgacg gctcttttgc tcaaggagca gggctacgat gtgatcggta
     4561 tcttcatgaa gaactgggat gacacagatg aaaacggcgt ctgtacggcg accgaagatt
     4621 acaaggatgt ggttgcggtg gcagaccaga ttggcattcc ctactactct gtcaattttg
     4681 aaaaagagta ctgggaccgc gtttttgagt atttcctagc ggaataccgt gcagggcgca
     4741 cgccaaatcc ggacgttatg tgcaacaagg aaatcaagtt caaggccttt ttggactatg
     4801 ccatgacctt gggggcagac tatgtagcga ctgggcatta tgctcgagtg gcgcgtgatg
     4861 aggacggcac tgttcacatg cttcgtggcg tggacaatgg caaggaccag acctatttcc
     4921 tcagccaact ttcgcaagaa caacttcaaa aaaccatgtt cccactagga catttggaaa
     4981 agcctgaagt tcgaaaacta gcagaagaag caggcctttc gactgctaag aagaaagact
     5041 cgacagggat ttgctttatc ggagaaaaga actttaaaaa cttcctcagc aactacctgc
     5101 cagctcagcc tggtcgcatg atgactgtgg atggtcgcga tatgggcgag catgcaggtc
     5161 ttatgtacta tacaatcggt cagcgtggcg gactcggtat cggtgggcaa cacggcggtg
     5221 acaatgcccc ttggttcgtt gtcggaaaag atctaagcaa gaatattctc tatgtaggac
     5281 aaggattcta ccatgattcg ctcatgtcaa ctagcctaga agccagtcaa gtccacttta
     5341 ctcgtgaaat gccagaagag tttacgctag aatgtacggc taaattccgt taccgtcagc
     5401 ctgactctaa ggtgaccgtc catgtcaaag gagacaaggc agaggtcatc tttgcggaac
     5461 cacaacgcgc gattacacca ggacaggcag ttgtctttta cgatggcgaa gagtgtctag
     5521 gtggtggttt gattgacaat gcttaccgcg atggacaagt ttgtcagtac atttagattg
     5581 acaaattttc tcaatttgct acaataataa cagcaataga aatgatggtc aaagctcatg
     5641 gatgttgcag gcttttttgt cctgcacttc tttgtagttt tgactgtttt tgtgtcgttt
     5701 aagggaaagg acaagaatga ctcaacaaga ctttcggaca aaagtagaca atacggtttt
     5761 tggtgttcgg gcgacagctt tgattcttca aaatcgcaag cttctagtca ccaaagacaa
     5821 gggcaagtat tacattatcg gcggtgcgat tcaagttaac gaaaaaacgg aagacgcggt
     5881 agtccgtgaa gtgaaggaag aactgggtgt taaatctcaa gctgggcagc tagcttttgt
     5941 ggttgaaaat gttttgaagt ggacggcgtt tcctatcaca atatcgagtt tcattatctg
     6001 gtggatttgc ttgaagatgc cccgttgacc atgcaggaag atgagaaaag gcagccctgt
     6061 gagtggattg acttggataa gctccagaat atccagctag ttccagcctt tttaaaaaca
     6121 gccctaccag attgggaagg ccaactaaga cacattcatc ttgaggaata ggagagaaac
     6181 atgacttatc attttactga agaatacgat attattgtaa ttggtgcggg acacgctggg
     6241 gttgaggctt ccttggccgc tagccgtatg ggctgtaagg tcctgcttgc gaccatcaat
     6301 attgaaatgc tggctttcat gccttgtaat ccctctatcg gtggttctgc caaggggatt
     6361 gtcgtgcgtg aagtcgatgc cctcggtggc gagatggcca agactattga caagacttac
     6421 atccagatga agatgctcaa cacaggaaag gggccagctg tccgtgctct tcgtgcgcag
     6481 gctgacaagg aactttactc taaggagatg cgcaagacag ttgaaaatca agaaaatctg
     6541 acccttcgtc aaaccatgat tgatgagatt ttggtggaag atggcaaggt tgtcggtgtg
     6601 cgtacagcca cccatcaaga atatgctgct aaggctgtta ttgtgacgac agggactgct
     6661 ctccgtgggg aaattatcat cggagacctc aagtactcat caggtcctaa ccacagccta
     6721 gcttctatta accttgctga taatctcaag gaactgggtc tcgaaatcgg tcgtttcaag
     6781 acaggaaccc ctccacgtgt caaggcctct tctatcaact acgacgtgac agaaatccaa
     6841 ccaggagatg aagcgcctaa tcacttctca tacacttcac gtgatgagga ttatgtcaag
     6901 gaccaagtgc catgctggtt gacctatacc aatggtacca gtcatgagat tatccaaaac
     6961 aacctccacc gtgcgcctat gtttacaggt gtggtcaagg gagtggggcc tcgttactgt
     7021 ccgtcgattg aagacaagat tgtgcgcttt gcggacaagg aacgtcacca actcttcctt
     7081 gagccagaag gacgtaatac agaggaagtc tatgtgcaag gcctctcaac cagtctgcct
     7141 gaggatgtcc agcgtgactt ggttcattcc atcaaaggtt tggaaaatgc agagatgatg
     7201 cggacaggtt atgctattga gtatgatatg gtcttgcctc atcagttgcg tgcgactctg
     7261 gaaaccaaga aaatctcagg tctcttcact gctggtcaga caaatggaac gtcaggttac
     7321 gaagaggcag caggccaagg gattatcgcg ggtatcaatg cggctctgaa aatccaaggt
     7381 aaacctgagt tgattctaaa acgaagtgac ggttatatcg gggtgatgat cgacgacttg
     7441 gtgaccaagg gaaccattga accttaccgt ctcttgacca gtcgtgctga ataccgtctc
     7501 attcttcgtc atgacaatgc tgatatgcgt ttgacagaga ttggccgcga gattggcctt
     7561 gtggatgatg aacgctgggc tcgttttgaa atcaagaaaa atcaatttga taatgagatg
     7621 aaacgcctag acagtatcaa actcaagcca gtcaaggaaa ccaatgctaa ggttgaggaa
     7681 atgggcttca agccgttgac agatgcggtg acagccaagg aattccttcg ccgtccagaa
     7741 gtttcttacc aagatgtggt ggccttcatc ggaccagctg cagaagactt ggatgacaag
     7801 attatcgaat tgactgaaac agaaatcaag tacgaaggct atatttccaa agctatggat
     7861 caggttgcca agatgaaacg catggaagaa aaacgcattc cagccaatat cgactgggat
     7921 gacatcgatt ctattgcgac agaagctcgt cagaagttca aactcatcaa tccagaaacc
     7981 atcggccaag ccagccgtat ttcgggagta aacccagcag atatttctat tttgatggtg
     8041 tatctggaag gtaaaaatcg tagtatttct aaaactcttc aaaaatcaaa atgatacgtc
     8101 gtcggcttct tacgaatgag ttcaaagctt ggctttgatt catctccaac ctcccatagt
     8161 tccccgaact atgggagcta actcaagtta tgcattttcc tagccttctg gtctgatttt
     8221 cattgagttt taatctcaaa agaaagcata gagaaaaaca gctccgaaga cggggctgtt
     8281 ttgttgactt gtactcaatg aaaatcaaag agcaaactag gaagctagcc gtaggttgct
     8341 caaagcactg ctttgaggtt gt
//
LOCUS       DAVLVZ010000076         8274 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000076 DAVLVZ010000000
VERSION     DAVLVZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.076"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            118..1599
                     /locus_tag="VYN77_001777"
     CDS             118..1599
                     /locus_tag="VYN77_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1559201.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDSLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1960..2961
                     /locus_tag="VYN77_001778"
     CDS             1960..2961
                     /locus_tag="VYN77_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1559202.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3111..4028
                     /locus_tag="VYN77_001779"
     CDS             3111..4028
                     /locus_tag="VYN77_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1559203.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4039..4929
                     /locus_tag="VYN77_001780"
     CDS             4039..4929
                     /locus_tag="VYN77_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1559204.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4958..6574
                     /locus_tag="VYN77_001781"
     CDS             4958..6574
                     /locus_tag="VYN77_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1559205.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6584..7903
                     /locus_tag="VYN77_001782"
     CDS             6584..7903
                     /locus_tag="VYN77_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1559206.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
BASE COUNT         2771 a         1302 c         1567 g         2634 t
ORIGIN      
        1 tgttattata cgaaataaaa gattttagga aaagtcgtta tatcatgcta tacctattct
       61 ttgtggtata attgcaagag gtttaatccg ataatttata aagagagaaa gatattcatg
      121 agagatttat tatctaaaaa aagtcatagg caattagaat tattagaatt attatttgaa
      181 cataaacgtt ggtttcatcg ttctgaacta gcagagttat taaattgtac agaacgtgca
      241 gtcaaagatg atctatccca tgttaaatct gcttttcctg acttgatttt tcattcttct
      301 actaatggta tacgcattat taataccgat gatagtgata ttgaaatggt ttaccatcat
      361 ttctttaaac attcaactca tttttcgatt ttagaattca tcttctttaa tgaaggttgt
      421 caagctgaga gtatttgtaa agaattttat atcagttcat cttcgctcta tcgtattatt
      481 agccaaatca ataaagtgat taaaaggcaa tttcaatttg aagtcagtct gacccctgtt
      541 caaatcattg gaaatgagag agacattcgt tacttttttg cacaatattt ttcagaaaaa
      601 tattatttcc tagaatggcc atttgaaaat ttttcatcag agccactatc tcaattgtta
      661 gaattggttt ataaggaaac aagctttcca atgaatttgt caacgcatag aatgctaaag
      721 ttgctcctag ttacgaacct atatagaata aagtttggtc attttatgga agtagataaa
      781 gattctttta acgaccaaag tttggatttt ttaatgcagg cagaaggaat agaaggtgtt
      841 gctcagagtt ttgaatcaga atacaatatc tctttagatg aagaagttgt ttgccaatta
      901 tttgtgtctt actttcaaaa aatgtttttc atagatgaaa gtctctttat gaaatgcgta
      961 aaaaaggata gttatgttga aaaatcttac catctattga gtgattttat tgatcagatt
     1021 tcagtcaagt atcagattga aatggagaat aaggatagtc tgatttggca tctgcataat
     1081 accgcacatc tgtatcgtca agagttgttt actgagttta ttttatttga tcaaaaaggg
     1141 aatacaatca ggaactttca aaatattttt ccaaaatttg tttcagatat aaaaaaagag
     1201 ctttctcact atttagagac tcttgaggtt tgttctagtt caatgatggt aaatcatcta
     1261 tcttatacct tcatcactca taccaaacat ttggtgatta atttgctaca aaatcagcct
     1321 aaactaaagg ttttagttat gagtaatttt gatcagtatc atgcaaaatt cgttgcagag
     1381 acactttctt attactgtag caataatttt gaactggaag tttggactga attagaatta
     1441 tcaaaggaat ctttagaaga ttcatcttat gatatcatta tttctaattt tattattcct
     1501 cctattgaaa ataagagact catctattca aataatataa acacggtctc actcatatat
     1561 ttgttaaatg ccatgatgtt tattcgatta gatgagtaac caaaaataac aaaaaaacgc
     1621 tatttcgcgt ttgaactgta cctcaaaagt tagacagaaa aaatctaatt tttggggtgt
     1681 ttttgttaag aaaagacctt caatcattta agcaaaatgt acatctagat ttatagccct
     1741 ctaaaactct tgcttttttt gtagaagata caaaaaagcc ataaaaccaa ggcttttcct
     1801 gttttatgta gatgtttaca catgaaaatt gaagtatttt caaaagctaa ttggagtgat
     1861 tttattcatg atgggataaa tgatctggcc aaaaatgatt gagaaatagt tgcttaaaaa
     1921 gcatttgcat gatactatat attgaaagga ggtgataata tggtagaaca aagaaaatca
     1981 attaccatga aagatgttgc tttagaagca ggagttagtg ttggaactgt ttcacgtgta
     2041 attaataaag aaaaaggcat taaagaagta actttgaaaa aagtggaaca agcgattaaa
     2101 actttgaatt acattccaga ttactacgct agaggaatga aaaaaaatcg aacagaaacg
     2161 attgcaatca ttgtaccaag tatctggcat cccttctttt cagaatttgc tatgcatgtg
     2221 gaaaatgaag tctataagag aaataacaaa ttactcttat gttctatcaa tggtacaaat
     2281 agagagcaag actatctgga gatgttgcgt cataataaag ttgatggagt ggttgccatt
     2341 acctatagtc caattgaaca ttacttgacg tcaggaattc cctttgttag tattgaccgc
     2401 acatactcag atattgccat tccttgtgtt tcatcagata atgatgcagg tggaagagaa
     2461 gcagctaaac aattaattag taaaggatgt cagcatttag cctttgtggg cggacataat
     2521 acaaccatta atgaaaccaa acgtcgtaga atttcatttg aaaagtatgt ccaagagcat
     2581 aaaatacctc ttagtatttt tgatttggat gagacagtta ctgactatta tggaaagcta
     2641 gagagtttct tggaagaaaa ttcttctata gatggaattt ttacaatcaa cgattttaca
     2701 gctttggatg tgattgaaat actaaaaaaa aatggcaaac gtattcctca tgatgtccaa
     2761 attattggat acgatggaat taaaatggct ggagatagag attatctact ttcaactatt
     2821 gaacaaccat tggaagaaat ggcaaaagaa gcggttcgta ttttgtttga tataattgat
     2881 ggaaagactg ttaatttgca gacaatccta ccagtaaaat ttgttgaagg aaaaacaaca
     2941 aaaaatgaaa ataagtcttg acagaaagcg ctatcaatga tagaatgaat tcatataaaa
     3001 agatttattt ttaaaacaaa aatgaaacgt ttcaaaaaaa gaaataaaga gacagcgtca
     3061 agcgctatct tttctagaac aaaaatgaaa cgtttcaaaa ggaggttgct atgaatagca
     3121 aagcgaagca agtttctctt tgggaaagaa tcaagaaaca aaaactcttg ttattgatga
     3181 ctgtccctgg tttagtttta acctttatct ttaaatacat ccctatgtat ggggttttaa
     3241 tcgcatttaa agattacaat cccttaaaag gaattttagg gagtgattgg attggttttt
     3301 ctgagtttac aaaattcata tcctctccca actttggtat cttgttagcc aacacattaa
     3361 aattaagtat ctatggttta ttgcttggct ttttaccacc aatcattctt gctattatgc
     3421 tcaatcaact cttgagtgaa aaagtcaaaa aacgaattca gctcatttta tacgcaccaa
     3481 actttatctc agtcgttgtt attgtcggta tgattttcct cttcttttca gtgggaggac
     3541 caatcaacaa ttttctttct atgtttggaa tgaaggctga cttcttgaca aatccagact
     3601 tctttagacc tttatacatc tttagtggta tctggcaagg aatgggctgg gcttcaacgc
     3661 tctacacggc aacattggta aatgtagatc cagccttagt agaagcagct cgactggatg
     3721 gagccaatat cttccaacga atctggcaca ttgatattcc agctcttaag cctattatgg
     3781 ttatccaatt tgttttagct gcaggtggaa ttatgaatgt cggatatgaa aaagcattct
     3841 tgatgcagac atcgttaaat ttgccaactt ctgaaattat ctcgacatat gtctataaag
     3901 ttggtcttgt atcaggagac tattcttact caacagcggt tggtttgttt aatgcagtga
     3961 ttaacgtagt attgcttgtt gcagttaacc aaatcgttaa acgcatgaat aatggtgaag
     4021 gaatttaagg agggaagtat gaaaaattcg attatggata caaaatttga tagacgtatc
     4081 ttactcttaa ataaaatcat tattgtcttt atcgttttga tgactttgct tcctttactt
     4141 tatatcgtcg tagcatcctt tatggatcct aaggttctgg ttagtagagg gattagcttt
     4201 aatccagccg attggactgt agaaggttat cagcgtgtat tcagtgacca atctattcta
     4261 agaggtttta tcaattctct actatactct tttggatttg cagctttaac agtattgcta
     4321 tctgtgttta cagcttatcc tctttctaag aaagacttgg ttggacgtcg ttggattaac
     4381 tacttcttga ttgtgactat gttctttggt ggtggtttag tcccaactta cttgctcgta
     4441 aaagaattgg gaatgctcaa tactccatgg gctatcattg ttccaggtgc tgtcaacgtt
     4501 tggaatatta ttcttgctag ggcctatttc caaggattgc ctgaagaatt agttgaagct
     4561 gctgtcattg atggtgcaaa tgatttacag attttcttca aaatcatgct tcctcttgca
     4621 aaaccaatta tgtttgttct cttcctctat gcttttgtag gacagtggaa ctcatacttt
     4681 gatgcaatga tttatatcaa ggatccaaac ttggaaccat tgcaacttgt acttcgtaaa
     4741 attctcattc agagccaacc aggtcaagac atgattggag cacaagcggc tatgaatgaa
     4801 atgaaacgtt tagctgaatt gattaaatac gcaactattg tcatttccag cttgccattg
     4861 attgttatgt atccattctt ccaaaaatac tttgataaag gaattatggc tggttcactt
     4921 aaaggataaa aaaagaaaaa ataaaaggag ttttctcatg aaattcaaaa cattctcaaa
     4981 atcagcagtt ttgttgacag ctagtttagc agtacttgca gcctgtggct caaaaaatac
     5041 agcttcaagt ccagattata agttggaagg tgtaacattc ccgcttcaag aaaagaaaac
     5101 attgaagttt atgacagcca gttcaccgtt atctcctaaa gacccaaatg aaaagttaat
     5161 tttgcaacgt ttggagaagg aaactggcgt tcatattgac tggaccaact accaatccga
     5221 ctttgcagaa aaacgtaact tggatatttc tagtggtgat ttaccagatg ctatccacaa
     5281 cgacggagct tcagatgtgg acttgatgaa ctgggctaaa aaaggtgtta ttattccagt
     5341 tgaagatttg attgataaat acatgccaaa tcttaagaaa attttggatg agaaaccaga
     5401 gtacaaggcc ttgatgacag cacctgatgg gcacatttac tcattcccat ggattgaaga
     5461 gcttggagat ggtaaagagt ctattcacag cgtcaacgat atggcttgga ttaacaaaga
     5521 ttggcttaag aaacttggtc ttgaaatgcc aaaaactact gatgatttga ttaaagtcct
     5581 agaagctttc aaaaacgggg atccaaatgg aaatggagag gctgatgaaa ttccattttc
     5641 atttattagt ggtaacggaa acgaagattt taaattccta tttgctgcat ttggtatagg
     5701 ggataacgat gatcatttag tagtaggaaa tgatggcaaa gttgatttca cagcagataa
     5761 cgataactat aaagaaggtg tcaaatttat ccgtcaattg caagaaaaag gcttgattga
     5821 taaagaagct ttcgaacatg attggaatag ttacattgct aaaggtcatg atcagaaatt
     5881 tggtgtttac tttacatggg ataagaataa tgttactgga agtaacgaaa gttatgatgt
     5941 tttaccagta cttgctggac caagtggtca aaaacacgta gctcgtacaa acggtatggg
     6001 atttgcacgt gacaagatgg ttattaccag tgtaaacaaa aacctagaat tgacagctaa
     6061 atggattgat gcacaatacg ctccactcca atctgtgcaa aataactggg gaacttacgg
     6121 agatgacaaa caacaaaaca tctttgaatt ggatcaagcg tcaaatagtc taaaacactt
     6181 accactaaac ggaactgcac cagcagaact tcgtcaaaag actgaagtag gaggaccact
     6241 agctatccta gattcatact atggtaaagt aacaaccatg cctgatgatg ccaaatggcg
     6301 tttggatctt atcaaagaat attatgttcc ttacatgagc aatgacaata actatccaag
     6361 agtctttatg acacaggaag atttggacaa gattgcccat atcgaagcag atatgaatga
     6421 ctatatctac cgtaaacgtg ctgaatggat tgtaaatggc aatattgata ctgagtggga
     6481 tgattacaag aaagaacttg aaaaatacgg actttctgat tacctcgcta ttaaacaaaa
     6541 atactacgac caataccaag caaacaaaaa ctagaggttg attatgggag ataagaaata
     6601 cacagtagaa aaagccaatc gttttatagc agaaaataaa catctcgtta atactcaata
     6661 taagcctgaa gaacattttt cagctgagat tggttggatc aatgatccaa atggatttgt
     6721 ctattttcgt ggagaatacc atctctttta tcaattctat ccatatgata gtgtttgggg
     6781 gcctatgcac tggggacatg ctaaaagtaa ggacttggtg acttgggagc acttgccagt
     6841 ggcacttgct cctgaccaag attatgaccg aaatggttgc ttctcaggct ctgccattgt
     6901 caaggatgat cgcctctggc tcatgtatac tgggcatatc gaagaagaaa ccggtgtccg
     6961 ccaagtgcaa aatatggcat tttcagatga cgggattcac tttgaaaaga tttcccaaaa
     7021 tccagttgca actggagcag acttgccaga tgagttgatt gctgctgatt tccgtgatcc
     7081 aaaactcttt gaaaaagatg gacgctatta ctccgtagta gctgccaaac acaaggataa
     7141 tgtgggctgt atcgttctac tagggtccga taacctagta gaatggcagt tcgaatccat
     7201 ctttttaaaa gggggagaac accaaggctt tatgtgggaa tgcccagatt acttcgagtt
     7261 agatgggaaa gattgcctta ttatgtcacc catgcgttat cagcgtgagg gagactcata
     7321 tcataacatc aactcatcgc ttttgttcac gggtaaggta gattggggag aaaaacgttt
     7381 tatcccagaa tcagttcaag aaattgatca tggccaagac ttctatgcgc ctcaaacatt
     7441 gttggacgat caaaatcgtc gtatcctgat tgcttggatg cagacatggg ggcgtaccct
     7501 tccaacccat gaccaagaac acaaatgggc atgtgccatg actttaccta gaattctaag
     7561 attggaagat ggcaaactaa gacaattccc tgttaaaaaa ggccaatatc aaatccaaat
     7621 agataaagat tgtcattacc acttaggaaa tgatacagat tatcttgaat ttggttatga
     7681 cagtaatgcg cagcaagttt acattgatcg tagccatctt attcaaaaaa ttctaggtga
     7741 agaagaacag gacactagtc gacggtatgt agatattgaa gctaaagaat tggaagttgt
     7801 tctagataaa aattccatcg agatttttgt caatcaaggt gaagcaagct tgactgcaac
     7861 ttattactta acggtgccag ctgagctatc acgaattgat taaaaattaa gttatttctc
     7921 ctaaagaaaa agttctcttt ctaaaatagt ggaaagagga ctttttgtgt tttgggtata
     7981 taagcttagt ttatggtatt tgtaaaattg gtgttggatt atgatttaaa ctagttttct
     8041 aaagaatttg aaaaaaattt tatttaagca aaaaaacctt ggttccaagg cttttcctgt
     8101 tgtatttaga tgccccctac aagtatatta tgatacatag tattaagtga ttatttattt
     8161 ttgaggcttt tcttattcta aaagtaggta tatgagttta tggacattgt aattcacaac
     8221 aaattcacgg cagaaaatac aattaggtag tttgtaattt taaaattaat gcga
//
LOCUS       DAVLVZ010000077         8258 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000077 DAVLVZ010000000
VERSION     DAVLVZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.077"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            472..798
                     /locus_tag="VYN77_001783"
     CDS             472..798
                     /locus_tag="VYN77_001783"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559207.1"
                     /translation="MEKVDRDKLKIYIETHPDAYLTEIAAEFNCPPTTIHYALKAMGY
                     SLKKSRTYCEQDPEKVNRFLKELNHLSYLTPIYIYETGVETYFYLEYDRALSRQLVSL
                     EEDIII"
     gene            1032..1112
                     /locus_tag="VYN77_001784"
     CDS             1032..1112
                     /locus_tag="VYN77_001784"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1559208.1"
                     /translation="MLPLPTYSPEYNPIEKIWAYIKNISE"
     gene            1504..2810
                     /locus_tag="VYN77_001785"
                     /pseudo
     CDS             1504..2810
                     /locus_tag="VYN77_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2850..3860)
                     /gene="tsaD"
                     /locus_tag="VYN77_001786"
     CDS             complement(2850..3860)
                     /gene="tsaD"
                     /locus_tag="VYN77_001786"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1559209.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3850..4287)
                     /gene="rimI"
                     /locus_tag="VYN77_001787"
     CDS             complement(3850..4287)
                     /gene="rimI"
                     /locus_tag="VYN77_001787"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1559210.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMIAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREVDEG"
     gene            complement(4284..4967)
                     /gene="tsaB"
                     /locus_tag="VYN77_001788"
     CDS             complement(4284..4967)
                     /gene="tsaB"
                     /locus_tag="VYN77_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1559211.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNYSITLMPAID
                     FLMASLDWTPKDLGRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVAPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5145..5330
                     /gene="cibA"
                     /locus_tag="VYN77_001789"
     CDS             5145..5330
                     /gene="cibA"
                     /locus_tag="VYN77_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1559212.1"
                     /translation="MTNFDILNNQFLSLSENELSDIDGGLAPLVIFGVAVSWKVIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5332..5484
                     /gene="cibB"
                     /locus_tag="VYN77_001790"
     CDS             5332..5484
                     /gene="cibB"
                     /locus_tag="VYN77_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1559213.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(5697..5918)
                     /locus_tag="VYN77_001791"
     CDS             complement(5697..5918)
                     /locus_tag="VYN77_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559214.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            6300..6533
                     /locus_tag="VYN77_001792"
     CDS             6300..6533
                     /locus_tag="VYN77_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1559215.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6535..8214
                     /locus_tag="VYN77_001793"
     CDS             6535..8214
                     /locus_tag="VYN77_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1559216.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKAP
                     KGTFIEPNEIKDYPAGEILILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
BASE COUNT         2445 a         1875 c         1600 g         2338 t
ORIGIN      
        1 tatctaaatt tttataatca tcaataacct ttgaatctct agatacgatt atcaaaatag
       61 gatatagaga catccttctg atctgctttt tacaaagtcc aattatatgc ggatctatac
      121 ctccacaatg tccattatta tacctaacta taatatgagc cgaaaacact atatccttaa
      181 tgtctccata tccatcaggg atattaatat ttatttttcc acaactatat tgcattgtaa
      241 ccatctcctt aaacgacgca ttatgatatt tgatagagaa atttttatga ataactcaat
      301 aattttatag taaatcatgc ttatatctca aagataccta ttttctcttg tctcgacctt
      361 ctccaaagaa ttgctataat actattacaa atacatctgc actacacttc aaattttagc
      421 actgtataaa aacgtttcaa tacactaact tcaagaaaac ttccactatt aattgaaaaa
      481 gttgatagag ataaattaaa gatctatatt gaaactcatc ccgatgctta tttgactgaa
      541 atagctgctg aattcaactg tcctccaaca actattcatt acgctctaaa ggctatggga
      601 tatagtctaa aaaagagccg tacctactgc gaacaagacc cagaaaaagt aaatcggttc
      661 cttaaagaat tgaatcactt aagctacctg actcctattt atatttatga gacaggggtt
      721 gagacctatt tttatctcga atatgatcga gccttgagca ggcagttagt ctctctggaa
      781 gaagatataa ttatttgaat taagatcgag acaacgcaca ccagagattg cgatactgtt
      841 atagaagtac taatgccctt tttgtttcaa tatactatgg ctccgatgac ctataaagat
      901 acgatgacga gtgacttttt cgaagcttgc ttccaaaaat tcttactacc tactttagat
      961 acaccatccc ttatcattat ggacaatgca aggtttcaca gaatgaacat gtgtaaggag
     1021 cagggcatag actgttacca cttcctacct attcacctga gtataatccc attgagaaaa
     1081 tatgggctta catcaaaaac atctcagaat aatattgtca aattacgatg cttttcttga
     1141 ggcacttttg tcctattctt gtttcagccg actatactcc gttattgggc agctacggaa
     1201 cagtcgatgg gacgatgggg ggacataaaa aaatcctcca gttttgtttt ttataacagt
     1261 atactggaga attgacaatc tcggtagata cctcgttata gcgcggttac ttattaggca
     1321 gttacaaaac aactgtgaac agaaaacatt ccagagtcag acaagacttt ggaatgtttt
     1381 ggctctataa tttctgtagt gggtaatccc accccaggaa ttatagggtc gtttcttgta
     1441 gaaaaaaagc cccatatgac ctataatgaa aagcgtctaa ccaactcatt agaaagggtt
     1501 catatggaac aacttaagaa taccacagat ttgctcggat tgaaagacaa aaatatcaaa
     1561 atcttgtctg ttctgaaata ccaaacccat ctagtcgttc aggcaaagtt ggattccccc
     1621 gctcctcctt gtcctcattg tcaagggaag atgatcaaat acgacttcca gaaagcctct
     1681 aaaattccgc ttctcgactg tcagggttta cccacggtac tgcatctcaa aaagcgccgc
     1741 tttcagtgca agaattgcct taaggtggtc gtttctcaaa catccattgt caagaaaaat
     1801 tgccagattt ccaacatggt gagacaaaaa atcgctcagc tcctccttga aaagcagtct
     1861 atgactgaga ttgcccacag attggcggtc tcaacttcca ccgtcatccg aaaactgagg
     1921 gaatttaagt ttgaaaccga ttggaccaag ttgccaaaag ttatgagttg ggatgagtat
     1981 agcttcaaaa agagcaaaat gagcttcatt gcccaagatt ttgagtccaa atccatcctc
     2041 gcaattttag acgggcgaac tcatgcggtg attcgaaacc atttccaacg ctatcagaga
     2101 gaggttcggg agctggtcga ggtcatcacc atggacatgt acagccctta ttatcggctc
     2161 gctaagcaac tctttccaaa ggcgaagatt gttcttgacc gcttccacat tgtccaacat
     2221 ctgagccgag ctatgaaccg agtacgaatc caaatcatga accaatttga ccgaaaatcc
     2281 ttggagtatc gggcgctcaa gcgcttttgg aaccctcgct ttttcgtttc taggctcggg
     2341 ctaaatcagt ccactggact gatttactac accaggatag cttcaagctc tgtcagaaac
     2401 gattctatca gtccacgttt cgaatgcact taacccatcg ggaagtacga gataagctgc
     2461 tttcttactc tgagggatta caggttcact acgaactcta tcaactcctg ctctttcatt
     2521 ttcaagagaa gaatgccgac catttctttg gattgattga gcaagaactg ccaacggttc
     2581 atccgctttt tcaaacggtc ttttggactt ttttaaggga tagagataag attatcaacg
     2641 cacttaagct gccttattcc aacgctaaac ttgaagcgac caataatttg attaaggtta
     2701 tcaagcgcaa agcctttggt ttccggaact ttaacaattt taaaaaacgg attttgatga
     2761 ctttgaacat caaaaaagag agtacgaatt tcgtactctc cagattgtag cttttcgcct
     2821 acccactaca cttgacaaag agccactctt tattccatgg tatcaaaggc aagacttggt
     2881 ttggcattga ggtcccagcc tgcgaagttt tctttgttcc actcgctgac gctggcatag
     2941 gcaatcatac ctgcattgtc tccgcagagt cgcagagggg ggatgataac cttgacatct
     3001 gtgatttcgg ctgctaggcg ttctctgaga cctttattgg ctgccacacc acctgccaca
     3061 accagggttt taacaggata tttctccaaa gccttcttgg tttttgccat gagaatgtcc
     3121 ataactgctg cttggaagga agcacacaaa tcttctgtag acaggctttc tcccttttgc
     3181 tcggcattgt gataaagatt gataaaggca gatttcaaac ctgagaagga gaactccaga
     3241 ttatcttcct taatcatggc acgggggaaa tcataaatat cctgcccctg atgagccagc
     3301 tcgtcaatct cacgacctgc aggataggtc aagcccatga cacggccgac cttatcataa
     3361 gcctcaccaa ccgcatcatc acgggtttcc ccaacaatct tataatctcc tgcctccgaa
     3421 acataaacca actctgtgtg tccgccgctg accaagaggg ctagcaaggg aaactccaaa
     3481 ggctccacac tctgagctgc catgaggtgc ccagccatgt gattaacagg aatcagtgga
     3541 agtccgtgag cccaagcaaa ggccttggca gctgacaaac caactagcaa ggctccgacc
     3601 aagcctggtc cgtaggtaac cgcaacagct gtcacgtcct cttcggtaat ccctgcttct
     3661 gccaatgcct cctcgataca ggctgtaatg acctcgacat ggtgacgact ggctacttcg
     3721 ggcactacgc caccaaaacg tttgtgactc tcaatttgac tagcaatgac attggacaag
     3781 agctcatcgt cgtttttcaa gacggcgaca ctggtctcat cacaggatgt ctcaaatgct
     3841 aaaatatatc tatccttcat ctacttctct cttcatgata atggcgtcct cgactgggtc
     3901 atggtagtag gcctttcgct cagcgatgac cgccattttt tctttcttgt aaaatgcttg
     3961 cgctcgttga tttgactttc tgacttcgag gaaaatctcc ttatctgtcg gcaattgagc
     4021 aaacaaggct gacgcaattc cctgaccctg ataagctcct ttgacagcga tttgcaggac
     4081 ttctgcttca aaaagattct cctgcacagc tagaaatcca atcacttctg ccccatcata
     4141 agccaatgca taccaagtct ggtcttggga cagatctgct tggatttgct ccagagtcca
     4201 aggactgact gggtaaacag ctatcataac agcgtagatg gcttgagcta ggtcaggctg
     4261 ttgttgaatt cgcttgattt ctatcatagg cgtttaatgt aggactcacc agactcggta
     4321 tggttcttga gccagttttc ctcagcctcg actcgtttga ggtaattcgg cacaaaatca
     4381 tgcaaggagt ctgcttcctt gtcccaagct aaaagagcaa gattggctgc attaggcaag
     4441 gtttctttat aattagtcct tggcaagtgt tcttgaatct gctccacaaa gggggcaact
     4501 tctccgacaa aggttacctg actagcaccc ttgacttttt ctagcacctc ttcaaaagat
     4561 aggtgcgctt ctggcatgac aggtttagca ttttcataaa atcctgcata aacattgttg
     4621 cgacgcgcat ccatcaaggg gacaaacaaa ccttcttgtt ggtagggtac cagagccaag
     4681 aggctagaca taccaactaa ctcgatgttc agagtatgag ccaaagtctt ggcagtggct
     4741 accgcaattc gcaagcctgt atagctaccc ggcccttcag ctaccacgat tcggcccaag
     4801 tccttgggtg tccaatccaa acttgccatc aaaaaatcta tggcaggcat aagggtaata
     4861 ctgtaatttt tcttaatatt aatcgtcgtc tcggcaagaa cttgcttatc ctctaaaata
     4921 gcgagagaaa gagccttgct ggacgtatca aaagctaata ctttcataac acattcctat
     4981 ctttttgtct gcttactatt atactacaaa agctggcaca tgggaatttt ctttgccccc
     5041 agacaagagt gccctcactt aactaaaaat aatttaaaaa aatgctcact tttccttttc
     5101 ttttccgaat ataaaagtga acaagaaaaa aggaggaaag ttcaatgaca aattttgaca
     5161 ttcttaacaa tcaattttta tccttatctg aaaatgaatt atcagatatt gatggcggtc
     5221 tcgctccctt ggttatcttt ggagtagcag tatcttggaa ggttattgca ggtggaacag
     5281 cacttatagg ttctggtttg gcagctggtt attttttagg aggagattaa tatgatgaaa
     5341 gatttgaaca actatcgtga aatttctaat aaggaattgc aagaaatcaa gggtggcttt
     5401 ggtgtaggtg ttggtatcgc tttatttatg gcaggttata ccattggaaa agaccttcgt
     5461 aaaaagtttg gtaagtcatg ctagataaga aacacatttt tagaaggata aattttattg
     5521 tcttcatctc ttacagtttg ctcagcattc tcaatgattt gaacattact actatccctt
     5581 taccattcga tttatctgtt tgtattgttt tatttttatg cttcaattct atttttgaac
     5641 agaacaatga ctcccataaa aataataagc tttgaaaatt ccattgtcat atcattttag
     5701 aaaaatgcaa agaccacctc atcttgatag atggggtgga attttcgtgt cgtaaatcta
     5761 ctatctctac atccccaaac aaaaaacccc agcataagca gggcatctaa gcatttaatc
     5821 caaagtaaaa tacaaaccaa acgacatagg tcacgaggag gagaaaaagc gagtagagag
     5881 tcacaaaggt aattttcctc aagaacttgg tttgtcatcg ttccagtttg gcaaatagaa
     5941 gatttcctcc ataaacgcaa aacaaaaacg ataaaagcta gcaaacgaac tccgatagca
     6001 aaagcaaata agttatacat agggcaacct ccttgactta aaatctatat agaattatga
     6061 caagtaataa acttcacttc cgttatcaac ataacacatt ttctttattt ttgcaaacgc
     6121 ttaccgaaga aatcgtcccc taactttctc gtttccgtct tttactaatt tttcattttg
     6181 tggtataatt gaaataattg taacgaatca aggtcaatct agacacaaaa tggaatgaaa
     6241 tcaagcaaat atctgctaaa agtttggaat aagctgacct gtaaatagaa aggaactata
     6301 tgatttacaa agttttttat caagaaacaa aagaacgtag cccacgccgt gaaacaacac
     6361 gcgcgcttta cctagacatc gatgccagct cagaacttga gggccgtatc actgctcgcc
     6421 aacttgtcga agaaaatcgc ccagagtaca atatcgagta tatcgaactc ttgtctgaca
     6481 aattgctaga ttacgaaaaa gaaactggcg ccttcgaaat cacggagttc taatatggcc
     6541 tatactctta aacctgaaga agttggcgtt tttgccatcg gtggtctggg agaaatcggg
     6601 aaaaacactt acggaattga ataccaagat gagattatca tcgtcgatgc tgggattaaa
     6661 ttcccagaag atgacttgct tggtatcgac tatgtcattc ctgattactc ttacatcgtg
     6721 gacaatatcg accgcgtcaa ggctgttttg attacgcacg gacacgagga ccacatcggt
     6781 gggattccat tcctgctcaa gcaagcaaat gtcccgatct acgctggacc gcttgcgctg
     6841 gctttgattc gtggaaaact cgaagaacac ggcctcttgc gcaacgctaa actttacgaa
     6901 atcaaccaca acaccgagtt gacctttaaa aatctcaagg caactttctt tagaacgact
     6961 cactctattc cagaaccttt ggggattgtc atccatactc ctcaagggaa aatcgtctgt
     7021 acgggtgact tcaagttcga ctttactcca gttggtgaac ctgcagactt gcaccgtatg
     7081 gcagccctag gtgaagaggg tgttctctgt ctcctctctg actcgaccaa tgctgaggtt
     7141 ccaaccttta caaactctga aaaggttgtc ggccagtcga tcatgaagat tatccaaggt
     7201 atcgagggac gtatcatctt tgcatccttc gcctcaaata tcttccgtct ccagcaagca
     7261 acagaagctg ctgttaagac tggacgtaag attgcggtct ttggtcgctc tatggaaaag
     7321 gccattgtca acggaatcga gcttggttac atcaaggcgc ctaagggaac ctttatcgaa
     7381 ccaaatgaaa tcaaagacta tcctgcgggt gagattctga tcctctgtac aggtagtcag
     7441 ggcgagccca tggcagccct ctctcgtatc gccaacggaa cccaccgtca agtacaatta
     7501 caaccaggtg ataccgttat cttctcttct agtcccatcc ctggaaacac tactagcgtc
     7561 aacaagctga ttaacatcat ttctgaagct ggtgtcgaag ttatccacgg taaagtgaac
     7621 aatatccata catctggaca cggtggtcag caagagcaaa aactcatgct ctgcttgatt
     7681 aagccaaaat acttcatgcc tgtccacggt gaataccgca tgcaaaaagt ccacgctgga
     7741 ctagcagtgg atactggtgt tgagaaggac aatatcttta tcatgagcaa tggcgatgtg
     7801 cttgccctta ctgctgactc agctcgtatc gcaggtcatt tcaacgccca agatatctat
     7861 gtcgatggaa atcgtatcgg tgaaattggc gcagctgtcc tcaaagatcg tcgcgatcta
     7921 tctgaagacg gtgtcgttct ggcagtcgca actgttgact tcaaatcgca gatgattcta
     7981 tctggtccag atatcctcag ccgaggcttt gtctacatga gagagtctgg cgacttgatt
     8041 cgccaaagcc agcgtatcct cttcaatgcc attcgtatcg cactgaaaaa taaggatgct
     8101 agcgtgcaat ctgtcaatgg tgccattgtc aacgctattc gccccttcct ctatgaaaat
     8161 accgaacgtg aaccgatcat catcccgatg atcctcacac cagatgaaga ataatactct
     8221 tcaaaccacg tcagcttcgc cttgccgtat atatggtt
//
LOCUS       DAVLVZ010000078         8074 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000078 DAVLVZ010000000
VERSION     DAVLVZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.078"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(1..158)
                     /locus_tag="VYN77_001794"
                     /pseudo
     CDS             complement(1..158)
                     /locus_tag="VYN77_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(204..437)
                     /locus_tag="VYN77_001795"
     CDS             complement(204..437)
                     /locus_tag="VYN77_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1559217.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(443..1435)
                     /gene="plsX"
                     /locus_tag="VYN77_001796"
     CDS             complement(443..1435)
                     /gene="plsX"
                     /locus_tag="VYN77_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1559218.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIVGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1432..2202)
                     /gene="recO"
                     /locus_tag="VYN77_001797"
     CDS             complement(1432..2202)
                     /gene="recO"
                     /locus_tag="VYN77_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1559219.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2199..3368)
                     /locus_tag="VYN77_001798"
     CDS             complement(2199..3368)
                     /locus_tag="VYN77_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1559220.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAVFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3517..4527
                     /locus_tag="VYN77_001799"
     CDS             3517..4527
                     /locus_tag="VYN77_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV1559221.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDVGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            complement(4640..4720)
                     /locus_tag="VYN77_001800"
                     /pseudo
     CDS             complement(4640..4720)
                     /locus_tag="VYN77_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4755..5192)
                     /locus_tag="VYN77_001801"
     CDS             complement(4755..5192)
                     /locus_tag="VYN77_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1559222.1"
                     /translation="MSQEFINSSDGVIRQYLVTSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5277..7910)
                     /gene="polA"
                     /locus_tag="VYN77_001802"
     CDS             complement(5277..7910)
                     /gene="polA"
                     /locus_tag="VYN77_001802"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1559223.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNMSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDQLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
BASE COUNT         2232 a         2066 c         1484 g         2292 t
ORIGIN      
        1 cttctctcct tagatagcgc atgattacag gctttatctt caactgttag cggcttgagt
       61 ttgctggatt tacgtggagt ttgtgcttga ggatatatct tcatgagccc ttgataacca
      121 ctgtcagcca agattttacc agcttgtccg atatttctca tttctaaaaa ccatctactt
      181 ccgttgactt ggaactccga ttgctatttt ccttgaatga ttttaaccac atctcctacg
      241 ttttggagtt ggtcaatttc ttcatcgctg atttcgatac taaattcatc ttccagagtc
      301 aagataaact ccatcaagtc aacagaatcc gcatccaaat cgtctttcag actcaaggat
      361 tctgtcacga caaagtcctc tccctgtcgc tcttggataa tggtcacaat acggtcaaaa
      421 atttcttttt ctgtcatctc ttttattctc ctgaaaattc acgcgcagtc tgggcaacca
      481 cgtctgtttc tagcatggta cggatctgac gaatcgtact ataaacagcc ttggcatcgc
      541 ttgagccatg agtcttgaca acaggtgcct taacaccaaa caagaccgct ccaccaacat
      601 ctgaataatt gagctgtttt ttcaaaccac tgaggctgtc cttgagaagg agggcaccta
      661 gtttcgctcg aagaccacca cctgtaatag ctgtcttgag caagcccacg attcccatag
      721 ctgtcccttc gatggatttg agcacagcgt ttcccgtgaa accatctgcc acaacaacat
      781 ctgcaacgcc attcatcaaa tcacgcgctt ccacgtttcc gataaagttc aaactttcat
      841 cagccgccag taattcataa gtttccttac gaagcgggtc gcccttgcta ctctctgttc
      901 cgttgttgag caaaccaacg cgtggttgcg caatgccacg gacattttta gcatagaagg
      961 aacctagaac cgcatattga tggaggtgct gggctgtatt ttctgcattg gcaccaaggt
     1021 ctagcatgtc aaaacctttt ccatcaacgg taggcaaggt agacatgagt ccaggacggt
     1081 cgatattctt gatacgaccc acgatgaaga atcctgctgc caacaaggca cctgtattcc
     1141 cagccgaaag gacagcgtct gcttcaccat ctttgacagc cttggctgcc aataccatac
     1201 tggcattttt cttattccta atagctctcg taggttcatc atccgaatca atcttctcat
     1261 ccgtatggat aatgctgacg cgctctgtcg ctgtcagata ttgcttgatt ttagcttcat
     1321 ctccgtaaag ttgaacctcg atatctgaaa agtcagatag ggcttgattg acaccctcaa
     1381 caatggcctg aggtgcgtaa tcgcccccca tggcatctac tgcgattttt ttcatttctt
     1441 ttcctctttt agtaattgtc cccagtctgc tagggaatca ataaatttct ttgattttag
     1501 gtgaatccca acgtactctt catataattg atccataaat tggcgtagct cttgcttgat
     1561 tccaggcttg agcgaaatgg tctccaaagt ctcaaaatca atagcttgaa attgattgag
     1621 cagatagggg atattgggat tgagatgaca acgtctctca tcctcatgat aatgctctgg
     1681 acagaggcag gctccatatt tgaaagaaaa gtcaaaagcc tgaccaaccc gatggcagaa
     1741 gacacactca ttaaaattga ggctgattcc aaatcgagtc aaaatttgaa tttcaaaaat
     1801 attggtcaaa acctgataat ccaagcctgc ttccatcaac tccaaagtct tttgcaaaaa
     1861 agcaaacaag ggagcatcct gctgattgtc ctgcaaacta gcatctgcaa gagctgccac
     1921 ataggtcgca taggccatga caaagaggtc actattaatc ttgggaaaag tcatgacctc
     1981 atgatagtct tcgatgtaac tgagtccgtc atcattgatt cgcaagagaa atcgtgccaa
     2041 caccaagggc tgaataacag gcgccagctt agactgacca gcgtgtttga caaaaaacat
     2101 gcgtttgcca acctgctctg taaaaatttt gacgagcttg tcatcctcac gaaaattgcg
     2161 attgtaaagc accaagcctt gactcgtgat agactgaatc atgcttctct catgtactcc
     2221 tcaagtcgtt tcatggcttc tttgatagtc tccatgctgg ctgcataaga taggcggacg
     2281 tagccttccc cgtaacgtcc aaaggctgca ccagggataa aggcaacggc cttcttctga
     2341 gcaaaatcct tcagaaaagc aaaggagtct tgattgtagc ccgctggaat tttagcaaaa
     2401 atatagaagg caccgtctgg tttgataatc tcaaaaccaa gagcagtcat tttttcgatg
     2461 atatagtccc gacgctggat atattccttc ttcatgggct ccgcatcatt tttaccagcc
     2521 gtcaaggctt ctaccgcagc atgttgcgcc atggtatttg cggcagtgac caagtattgg
     2581 tgactcttga ttaactgggc tgtgaagacc gcaggagcga aaatcagccc caaacgccaa
     2641 cctgtcatgg catgcgattt agacaaacca ttgataataa tagcctggtc tctcaacatc
     2701 gttcctagag acacatgggc ttcgcctgtg taggtcaatt ctgagtaaac ctcatcacag
     2761 acaacaaaaa tttcgtactt gcgtaaaaca gctgccaagg cctctaactg ctctcgactg
     2821 taggtaattc ctgtcggatt ggctggatag ttgagaataa ccgccttgag cttatcaccc
     2881 tgctccaaaa tggccttctc caacatctca ggagtcaaga caaaaccatt ttcagtcgta
     2941 tcaatctcaa caatttctgc cccaactaag ttaacaatcg gttcatagcc tggataagca
     3001 ggagctggta aaagtacctt gtctccctct tccaaaatag ccgtcaaagt cgcagataaa
     3061 gcctctgtcg ccccaattgt aaccaagatt tcattttcag gagcatagtc cagttggtac
     3121 ttttccttaa caaagtcact ggctgcctga cgtagagtca gcagaccact catccctgta
     3181 tagtaggatt ggttctgatc aatcgctcgc ttggccgcct ccttgacatg gtctggcgtt
     3241 gtaaaatcag gttcccccaa ggtcaaacgc aagaccccag gaatctccga aataacctgg
     3301 tcaaactgac gaatcaacga aacttgaatt ttatctaact gtttattaaa gcgcttagtt
     3361 aagtccatag atacctccta ctttcaaaac gtatctctat tatactacaa aagccccccg
     3421 accgcaaaaa tacattagag aatagctttc cactttctat tttacttttc ctgtttacag
     3481 gtctataatg gtaacagatt ctatttggag gtttttatgt catttctatc aaaaaatgga
     3541 gcaggcatct tggtctgcct tctcatttct atcgtatctt ggtacttagg aggattcttc
     3601 cctgttattg gcgcacccgt ttttgcgatt tttatgggca tgctcctaca tccctttctc
     3661 tcgtcttata aacaactgga tgttggattg acctttagtt ccaagaaatt gctccagtat
     3721 gccgttatct tacttggttt tggtctcaat atctctcaag tcttcgcagt tgggcaatct
     3781 tcactccctg tcatcctttc caccatttca atagccttga ttgttgccta cctcttccag
     3841 cgcttctttg ccctggatac aaaactggct accttgattg gagtaggttc ttctatctgt
     3901 gggggttctg ccattgcagc gacagcgcct gttatccatg ctaaggaaaa ggaagtagcc
     3961 caagccatct ccgttatctt tttcttcaat gtcttggctg cgctcatctt tccaaccctc
     4021 ggcacctggc ttcatctatc caatgacggc ttcgccctct ttgcaggaac tgcggtcaat
     4081 gatacttcct ctgtaaccgc cacagctagc gcttgggaca gtctctacca gaccaatacc
     4141 ctcgaatccg caaccattgt caaactcacg cgcaccctag ctatcatccc catcactctc
     4201 tttctctcct actggcaaag tcgcgagcaa aaaaataagc aaggcttaca actgaaaaaa
     4261 gtcttcccac tttttatcct ttacttcatc cttgcatcgc tcctaaccac ccttctgacc
     4321 tctctcggtg tgtctagtag tttctttacc cctctcaaac aactctctaa attcctcatt
     4381 atcatggcca tgagtgccat cggtctcaaa actaatctgg tcgctatggt caaatccagt
     4441 ggaaaatcca ttgttctcgg agccgtctgc tggattgcca tcatcctcac cagccttggt
     4501 atgcagaccc ttatcggcat tttctaatac tcttcgaaaa tctcttcaaa ccacgtcaac
     4561 gtcgccttgc cgtatatatg ttactgactt cgtcagttct atctgcaacc tcaaaacggt
     4621 gttttgagct gacttcgtca gtcgtatcta caacctcaaa acagtgtttt gagcagcccg
     4681 cggctagttt cctagtttgc tctttgattt tcattgagta taacacaaaa ggtagcccat
     4741 cagctacctt tttcttatgc ttcctcaatc aagcgagtat gttctctctt gatacagcga
     4801 ttcatcacga tatcatcaca tccaccatca cgcaaaatct ctttcgcttc taaactttca
     4861 agtcctagct gtgcccaaaa aatcttggca tcagctttga gaaaatcacg cgccacatcg
     4921 ggcagaaatt cactgcgacg ataaacattg acaatatcta caggaaaagg aatttcagcg
     4981 aggctagcat aagccttttc acccaagatt tcgccacctg ccgccttggg attgactggg
     5041 atgattttat agccccgagc ctgcatttcc tttgtcactc gattgctggt tgtttcttca
     5101 cggtcagaca aacccaccac agcaagggtt ttactcgtta cgagatactg acgtatcacg
     5161 ccatcacttg aattgataaa ttcttgactc atagaaatcc tcctttttca tcagtatagc
     5221 acattttgaa aaggtttgca gaattctact acaaaaaagg aggactagcc ccctttttat
     5281 ttagcctcgt accaggttgc tccttcattc tcatctgcga taagaggaac actgagttga
     5341 atggcttctt ccatggtttg tttcaccaat tttttcatct ctaccaattc agatttaggc
     5401 acttcaagga cgatttcatc gtgcacttgt aacagcatct tagtctgata accacctgca
     5461 accaaggctt tatccagctg aatcatggca atcttgagaa tatctgctgc cgaaccctgg
     5521 ataggtgagt tgatagcagt tcgctccgca aaaccacgaa tattgaagtt gcgcgaattg
     5581 atatctggca actcacggcg acgcttaaag agggtctcta catagccctt atcacgcgcc
     5641 tcccgcacca cttcatccat gtagttttta atacctggaa aacgttcaaa gtaggtatca
     5701 atgtaggctt tggcttcctt acgactaatt cccaaattat tagacaagcc aaagtctgaa
     5761 atcccataaa ccactccaaa gttaactgcc ttggcattgc gacggtcatt tgcagtcaca
     5821 ttatcaggac gctcaatgcc aaagacccgc atggctgtcg aagtatggat atctgccccc
     5881 tcttggaagg ccttaatcaa gtgctcatcc ttagaaatat gcgccaaaac gcgcaattca
     5941 atctgtgaat agtcagagct gagtagcaca ctatcctccc actctggcac aaaagccttc
     6001 cgaatcaagc gcccttgttc caatcgggca ggaatatttt gcaagtttgg atccacacta
     6061 gacaaacgcc cggtctgggt caaatcctgc atatagcgag tatgaatctt tccatcagcc
     6121 aaaatccagt cctgcaagcc aattacataa gtagattgaa tcttagcaat ttgacggtaa
     6181 tccaggattt tcttaacaat cggagcaata ggagcgagac gctccaacac atccactgct
     6241 gtcgaataac ctgtcttggt tttcttagtg tattctagag gaagtcccaa tttctcaaag
     6301 agaagcacgc ccaactgctt aggcgagttg acattaaact cctcaccagc cagctcgtaa
     6361 atctcttgag tcagtttttc aatgacaagc tcattttcag cctgcatctc aagcaaggtc
     6421 tctttcttga ccacaatccc agcaatttcc atcttggcaa ggacaaaagc cagaggttgc
     6481 tccatatcat aaagaagctc taattgccca ttttcgctaa gtttttcaag taaaataggc
     6541 tctgtttcta ccaaaacagc aagtttacaa gctaagtgtt ccaagaattt ctcacgttca
     6601 ggaatggcct ttttaacacc cttaccgtag aaagtttcat catcaaccaa gtaagtctga
     6661 ccataaagac tagcgatggt cgcaatttca ttgtcctcca cagtcgaaag gaggtattta
     6721 gccaaacgga tgtcaaaagc aggcgcctgc aaatctacac caaaacgttg caaaagaact
     6781 ttaaccttct taaagtcata aactctcaga gatgtttttt ctaagaaatc cttgaaaatc
     6841 gggtcttgca acagctcaag cttgtctgtg gcatagagct gatccccaca agaccaggca
     6901 aatccaacca aattatctgt atggtaattc tcaccaaaaa gctcaaagtg gaagatagac
     6961 tcttcactca gcatatcttg actgatttgg tcaacaatag taaaatccaa accctcagcc
     7021 acatcagctg acgacatatt taaagcctgc ttgagctgtt tgaagcccat ctcatcgtag
     7081 aatttcccaa gattttcaac atctggacca ctatagacca agtcctctaa accaatcgca
     7141 atcggtgcct tggtatcaat ggtcgctagt gttttagaca aaaaggcctg ttccttgtca
     7201 ttgatgagat tttccttcat cttagaagtc ttcattccat caatattttc ataaatcccc
     7261 tcaagcgaac catgctccag caagagctta atacccgtct tttcaccgac tttggtcacc
     7321 ccagggatat tatccgactt atcacccatg agcgccttga gatcgataaa ctgagctggt
     7381 gtgaggccca tttcttccat gaggtaatct ggcgtaaagg cctcaaactc agccacacct
     7441 ttcttggaaa tttcaaccac cgtatgctca tccgtcagct gaatcaaatc cttgtcccca
     7501 ctgacaatgg taatatcaaa accatcctgc tctgctagct tatccagcgt cccaatgatg
     7561 tcatccgcct catactgagc cagctcatag tgacgaatcc ccatatgatc cagcaactca
     7621 cgaatgaaag gaaattgctc acgaaactca tcaggagtct tggcccgacc acccttatag
     7681 tccgcataca tctctgtccg gaaggtcgtc tttcccgcat caaaagccac caaaatatga
     7741 ctcggctcaa cccgctccaa caaatgactc aacatcaact gaaaaccata aatcgcattg
     7801 gtatgcaaac cagccgcatt cttaaaacgg tccaactgct gatacagcgc aaaaaacgcc
     7861 cgaaaagcta cagaagaccc atcaatcaat aataattttt tcttatccat acacccatta
     7921 taaaggaaag aatcaaaaaa taccattggg aagagctaga gcaagtattt ttcaaacttt
     7981 ttccgaataa atagatagag ccagagaatt tagtaaacct agatttaaaa atgtgctata
     8041 atatagtata ttgaatctat aatagtacac cttg
//
LOCUS       DAVLVZ010000079         7831 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000079 DAVLVZ010000000
VERSION     DAVLVZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.079"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            299..598
                     /locus_tag="VYN77_001803"
     CDS             299..598
                     /locus_tag="VYN77_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1559224.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVISIDFNPAVIDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            complement(940..1914)
                     /gene="pta"
                     /locus_tag="VYN77_001804"
     CDS             complement(940..1914)
                     /gene="pta"
                     /locus_tag="VYN77_001804"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1559225.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(1958..2854)
                     /locus_tag="VYN77_001805"
     CDS             complement(1958..2854)
                     /locus_tag="VYN77_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1559226.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(2851..3669)
                     /locus_tag="VYN77_001806"
     CDS             complement(2851..3669)
                     /locus_tag="VYN77_001806"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1559227.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(3653..4324)
                     /locus_tag="VYN77_001807"
     CDS             complement(3653..4324)
                     /locus_tag="VYN77_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1559228.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRSITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            4452..5021
                     /locus_tag="VYN77_001808"
     CDS             4452..5021
                     /locus_tag="VYN77_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1559229.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGQQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            5134..6093
                     /locus_tag="VYN77_001809"
     CDS             5134..6093
                     /locus_tag="VYN77_001809"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1559230.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            6103..7218
                     /locus_tag="VYN77_001810"
     CDS             6103..7218
                     /locus_tag="VYN77_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1559231.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETVFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLTGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            7223..7570
                     /locus_tag="VYN77_001811"
     CDS             7223..7570
                     /locus_tag="VYN77_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1559232.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            7578..7793
                     /locus_tag="VYN77_001812"
     CDS             7578..7793
                     /locus_tag="VYN77_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1559233.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
BASE COUNT         2462 a         1653 c         1448 g         2268 t
ORIGIN      
        1 ccaaaaatag atagaggtaa catgtttgca aacaaagcat acgaaccttt agtaaaatca
       61 tttccatgaa actagaatag agcccctctt agcaaaaatc attattttaa tttatttcta
      121 atcactcctt gacataaata actctcacca ataaaagact atgtcttaaa aaaatggtat
      181 aataaaatca atacttgcgc ttgatggcta tgctactaat aacaattagg agagaaaatc
      241 aggcacttgt taacaacaag gattatctcc ttgagatgaa aggaacttta gaaatcttat
      301 gatgaacatg caaaacatga tgcgtcaagc acaaaaactt caaaaacaaa tggaacaaag
      361 ccaagctgaa cttgctgcta tgcaatttgt tggcaaatct gctcaagatc ttgtccaagc
      421 gaccttaact ggagataaga aagttatcag cattgatttc aatccagctg tcattgaccc
      481 agaggacctt gaaactcttt ctgatatgac cgttcaagcc atcaactctg ctcttgaaca
      541 aatcgatgaa actaccaaga aaaaactggg tgctttcgct gggaaattgc cattctaaaa
      601 acaaggagct aaaacaatgc ttgtcgataa caaaggctaa gaaaggtgca aaaattataa
      661 aaaacgcata atagcaggtg tcaagacact taatattatg cgttttctca tgaaaaaatt
      721 ctctttccat actcatttaa aatcaaaaag ggaatttatc gttttacgga tatgtatatt
      781 acagatttct ataggtcgtg tatagacagg atatcatcgc ttggaagttt tcctttttga
      841 taccaatcag taatttagtt ctcaggaatt aatggcagtg cttttcgtac atagtttcat
      901 aactacagta tagcatagta tatcacttta tagttttcac tattgatgaa ctgcttgagc
      961 tgctgtgatg agggtcaact tgtaaacatc atctgcatta catccacgag aaagatcatt
     1021 aactggcttg tttaaacctt gcaaaacagg tcctacagcc gcaaagccac ccaggcgttc
     1081 agccatcttg taaccgatat ttcctgcctc gatacctggg aagatgaaga catttgcttg
     1141 accagctact gtacttcccg gagctttcag agctgcagtt tcgggaacaa aggccgcatc
     1201 aaattgcaac tcaccatcga tttcaaggtc aggacgcaag tcgtgagcaa ttttagttgc
     1261 ttcaacgacc ttatcaacgc tttcaccaaa ccctgaacct ttagtagaat agctcaacat
     1321 ggcaatttta ggttcgatgc caaacatctt agctgtgatt gctgagttga tggcaatttc
     1381 agccaaggct tctgcatctg gattgatatt aatggcacag tctccaaata ggtaacgttc
     1441 cgtaccacga accatgagga aggctcctga agtacgagtt acatttggac gagttttgat
     1501 gatttgtaga gctgggcgaa ctgttgaagc tgttgagtga atcgctcctg acaccattcc
     1561 atcaaccaag cccaagtaaa ccaacatcac accaaagtag ttgacatctt caaccaaaac
     1621 cttgcgtaca tcttcttcag tcattttgcc cttgcgacgc tccaccaagg cagaaaccat
     1681 ttcttcaaat tgaggataat gttgagggtc gatgacctca taaccatcca tgattccttc
     1741 aatttcaaga taaattttaa ttttttcagg atttccaagc aaaacaggaa tcacttctgt
     1801 ttcttttact aagcgttttg ttgcttgaag aatacgaggc tcttcccctt cagggagaac
     1861 gatacgagca tttttaccaa caaggttggc tttgagactt tcaaaaactt ccatgagttt
     1921 tctcctttaa gataataatt atactctgag acagttttta tagagtatta gttgataact
     1981 gggtaataag gttactgaaa tcatccggca agggactttc taactgcaag tcttgctcta
     2041 aaaatggatg ataaaaggat aggtaatggc aatgcagagc ctgacgttga ataccatctt
     2101 ccagactacc accatacaaa tcatctccca gcaaaggaaa accgatatga gaaaaatgga
     2161 ctcggatttg atgggttcga ccagtgtgca ggtgaatata gaccaagtga atatttccat
     2221 aagaagctac aatcttgtat gaagtatggg catactttcc gcctttagcc actcgtctgg
     2281 taataatgga atcttcatca cgcgcaatcg gagcaataat ttccccttct ggctccaaat
     2341 gtccatctcc cttaaccaaa gcaaagtagc gtttctcgat agatttcttc tgcaactgct
     2401 tgtctaatcg tgcatgggca taaccgtgct tggcaaagag catcaagcca gaagtgtccc
     2461 tatctagtct ggtaacaatg tgaacctgct gattttcata attttgcttg acatagtaac
     2521 ccttgataaa attggcaatg gtattagagt gattgacact aggaatagaa gccactccat
     2581 agggtttatt caagactaga aagtggtcat cctcatagag aatatctaat ggatgctcaa
     2641 tagcctccaa ggtttcaaag ccttcctcag cgggaatgta aatggtaacg tagtctccaa
     2701 cgtccaatag atacgttgca ttttgcggtt gattattgac cagaatagct ccacctcgaa
     2761 acttaatctt ggccagcaat cccttagaaa cctcgtgctt ttttaaaaag gtcttaacct
     2821 tgacatgttc atctgcgata aattcaaacc tcattcatcc acctcaccga taaaggcatc
     2881 cttaacacgg ttccagaaac tggtatggct aggagtcgcg acaaagtgaa tcttatgatg
     2941 gtcgatttga tactcaatac gctcaatatt acggaaagaa taaacgctat tgtcaaccga
     3001 aatagtatga taatcgtttc ttgttggaat aagttcaatc ttatccttct taggcacaat
     3061 aatggaagag cccagtgttc gataaacacg attattaagg ctggcaattt ccgttaattg
     3121 caaagcttca atggtagggt gtaaaacagc accgccaaga gacttgttat aggcagtact
     3181 accagtcggt gtcgaaactg ttagcccgtc tccacgaaaa cgttcaaagg gaacaccatt
     3241 tattacaata tctgccacca tggttcgatc agacctgcgg atgctggctt cgttgagtgc
     3301 tctgaaaatc ttaacttcac cattttcaag aaagaccttc acattcagaa cagggtaaga
     3361 aacccttgcc ccagtatcta gctgcaaatt agtcactagc ttgtccaact caaaatcacg
     3421 ataatctgta tagaagccca aatgtccagt atgaagaccg ataaagcgga ccttgtcaag
     3481 ctgattttcg tacttatgaa aggccgacaa gagcatacca tccccgccaa tggaaatgac
     3541 aatatccgga ttggtatcat tgagtataaa ctgatttctc ttcaaacgat ctcgcaattc
     3601 atacaaaacc ctttgactct gcggttttct attggctatc agatcaattc gtttacctgt
     3661 attcttcatc tgtatcgtca ctgtttccta caccgtcatt taattttcta ctcaaaggat
     3721 caaaaagtgc ctgggcttct tggatatcat cacgaatttc acccatttct tcatccaact
     3781 gatgggcgat tctagctgta atttccagtc gcttcttaat ctcatctggg aaatcccctt
     3841 ggtacttgta gttgagagaa tgttctatcg ttgcccagaa attcatggcc aaagtacgaa
     3901 tttgaatttc tgccaaaata gtcttagctc cattgatggt atcaaccgta tattctacta
     3961 ccacatgata ggaacgatag cctgatgctt ttctatgagt aatgtaatct cgctcctgta
     4021 tgattcgcat atcctgacgc ttgtgcaaaa tatccactac ttccttgacg tcatctacaa
     4081 actgaaccat cacacgtaag ccagcaatat cctgcaaatc gtgttccaag gtcgcataag
     4141 taatgctacg acgagccatt ttttctttga tgctctcaat tggcttgact cgaccggtca
     4201 caaactcaat tggagaatgc ttattttgct tacgatattg cttacgaata ccacgaagtt
     4261 taatctttaa ctcaccaaca gcttgaatgt aaggatctag aaattcttcc cattctaagg
     4321 tcatatattc tcccttgttc tcatattatc cgtcaaaaat atctttgatt ttttctccag
     4381 attcatatac aataaataca atgcttatta taccataaat ccgctttcaa cgataaaaaa
     4441 aggtaagaaa aatgaaacat ttagaaattg aattgaaaac actattgaaa aaagatgaat
     4501 acaatcgtct aaaagaccag ttcacaggtg tcactcctgt tcttcaaaca aattactaca
     4561 tcgacacgcc tgattttgaa ctgcgagaaa agaaagtcgc tatgcgcatt cgaacctttg
     4621 aagactgggc tgagttgaca ctcaaagtcc cgcagagtgt tggaaacatg gaatacaacc
     4681 aaaaattgca actaaaagat gctgagaact atctgagtaa ggaagaactt cctcaagggc
     4741 tagtactgga tgaattggcg aaacatggta tccaaagtaa gaactggcag gttcttggtt
     4801 gtctaacaac gcttcgctat gaaatgaaaa cagctattgg tctcatggca ctggatcaga
     4861 gccgatactt tgatatgaca gattacgaat tggagcttga agtggaaaat catgagcaag
     4921 gccaacagga tttccgacaa tttttagaga aaaatcagat ttcctaccaa aaagctcctt
     4981 caaaattggt tcgatttgtc aaaagtatga aaaatagctg aaataatctc cattttttgg
     5041 taaaatagaa aagataaaaa tacacaaacc ccagttcata tatgtaaggt aaatataact
     5101 aggatttata aagtttacag aggacggtct ataatgtcag atagaaaaaa catgaaactt
     5161 ttcgcactca actctaacca agagattgca caaaaaattg cccaagctgt tggtgtccca
     5221 cttggaaaac tatcatcacg tcaattttca gacggagaaa tccaagtaaa tatcgaagaa
     5281 agtgtccgtg gttatgatgt ttacatcatc caatcaacaa gtttccctgt caacaaccac
     5341 ctaatggaat tgttaatcat ggtcgatgct tgtgtgcgtg caagtgccca cagtatcaac
     5401 gttgtccttc catattttgg ctatgcacgt caagaccgca ttgcttgtcc tcgtgagcca
     5461 cttacagcaa aactagttgc caatatgctg gttaaggctg gagttgatcg tatcctgact
     5521 cttgatttgc atgccgttca ggttcaaggt ttctttgata ttccagtgga taatcttttc
     5581 actgttcccc tattcgcaaa acattactgc gataaaggat tgcttggttc agatgttgtt
     5641 gtcgttagcc ctaaaaattc aggtgtcaaa cgtgcgcgta gcctggctga atatcttgat
     5701 gctcctatcg ccattatcga ctaccctcaa gacgatgcaa ctcgtaacga aggttatatt
     5761 attggtgatg ttgaaggtaa gaaagctatc ttgattgatg atattttaaa tacaggacgt
     5821 accttctctg aagcttctaa aatcgttgaa cgtgaaggag ctacagaaat ttatgctgtt
     5881 tctagccacg gtctcttcgt cgagggagct gctgagcttc ttgacaatac taatattaaa
     5941 gaaattcttg tgactgattc agtagcaaca aaagaaaaaa ctcctaaaaa tgtatgctac
     6001 atcactgcta gtgagttaat tggtgatgct atcgtccgta ttcacgaaag aaaaccagtc
     6061 agcccactct ttgcctacaa taaaaagaaa taaggtgatt ctttgattta tttggacaat
     6121 gctgcaacga ctcctatgtc agcagttgct atttcagcta tgaccaaggt tatgcaagaa
     6181 acccacggaa atccttctag tattcatggt catggtcgtc aagctggcaa actcttgcga
     6241 gaagcccgtc aggaactagc ccagttacta aggacaaaac ctcaacatat ctttttcact
     6301 tctggtggga ctgaaggcaa taatactacc atcattggct actgtcttcg tcaccaagaa
     6361 caaggaaaac atatcatcac aactgccatc gagcaccatg ctgtccttga aacaattgat
     6421 tacttggttc aacactttgg gtttgaagca accattatcc agccagaaaa tcaagaaatc
     6481 acagcccagc aaattcaaaa ggctttacgt gacgatacga ttttggtttc tactatgttt
     6541 gccaataatg agacaggaaa cctacttccc atcgctgaaa ttggccaaat actcaagcaa
     6601 caccctgctg cctatcatgt tgatgcagtt caggctattg gtaaaatccc aattcattca
     6661 gaagaattgg gcattgattt tctcactgct tctgcccaca aattccatgg tcctaaggga
     6721 atcggttttc tctacgcatc tagcatggac tttgattcct atctacatgg cggagatcag
     6781 gaacagaaaa aacgtgcagg aactgaaaat ctgcctgcca tcgtaggcat ggttgcagcc
     6841 ctaaaagaag acctagaaaa acaagaagaa cattttcaac atgtacaaaa tctagaaact
     6901 gtctttctgg cagagctgga gggcattcag tattacttga atagaggaaa acatcatctc
     6961 ccttatgttc tcaatattgg atttcctggt cagaaaaatg acctcttact ccttcggcta
     7021 gatttaactg gaatttcaat ctctactggc tcagcctgta ctgcaggcgt tgtccaatcc
     7081 agccatgttc ttaaagccat gtatggcgca aattcagaac gcttgaagga atcccttcgc
     7141 atcagtttgt cgccacaaaa taccgttgaa gacctacaaa ccctcgcaaa aaccttaaaa
     7201 gaaattatcg gaggttagcc atatggcatt tgaaaaaatc attcagttaa aaaattgtcg
     7261 ttacgattac actcttagcc cttctgttaa aaaattcacc ctcaaagata acaccttttt
     7321 tgaaactaag gttggtaact atgaactgac tcgccttttg gaaaaagtgc caaacagcgg
     7381 tgaaggcttc caactcaaaa tcatcattaa caaggaactt acaggggcta aaatcaatat
     7441 cactgacaag tttggccttc gtctagttga tattttcaaa tcagaagacc accatattca
     7501 tcaggaaaaa ttctacttcc tcatggatag cttggtagaa cgtggtgtct ttacaaaatc
     7561 ggaaagatag agccaaaatg tttgaactga cctataagga ctgctatcat gtagagcgta
     7621 ctctcaagta tgaggatcat gaggctctca tgttgacttt gtcaggatgt gtaaccttac
     7681 cagatacact ttatgtgact tctctgactt ttaggggcaa aaaagtttat caggggctgg
     7741 tcggagacct ctaccgtttt ctatctcatg cagatttttt acatcaaaac taagattttt
     7801 cttagttttt tcttattttt gttgattttt t
//
LOCUS       DAVLVZ010000080         7667 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000080 DAVLVZ010000000
VERSION     DAVLVZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.080"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            150..449
                     /locus_tag="VYN77_001813"
                     /pseudo
     CDS             150..449
                     /locus_tag="VYN77_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            520..2541
                     /locus_tag="VYN77_001814"
     CDS             520..2541
                     /locus_tag="VYN77_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1559234.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            2543..3184
                     /locus_tag="VYN77_001815"
     CDS             2543..3184
                     /locus_tag="VYN77_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559235.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            3288..4640
                     /locus_tag="VYN77_001816"
     CDS             3288..4640
                     /locus_tag="VYN77_001816"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1559236.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIATD
                     KNTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            4644..5702
                     /locus_tag="VYN77_001817"
     CDS             4644..5702
                     /locus_tag="VYN77_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1559237.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            5712..6911
                     /gene="divIB"
                     /locus_tag="VYN77_001818"
     CDS             5712..6911
                     /gene="divIB"
                     /locus_tag="VYN77_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1559238.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEESES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     NQRSSRR"
BASE COUNT         2382 a         1315 c         1671 g         2299 t
ORIGIN      
        1 atatctaaaa aattcgattt tttccttgac aacaaataat aaaaaagtta ttcttatcac
       61 ataagaaagc ggtttcaata aaagagagag gaggatttat tttgttgcat tgttgctcaa
      121 ggtgtgcttt ttttgaaaga aaaaataaaa tgaaaacgaa aaaacataga ttacttgctc
      181 tagctcttat ttcaaatttt acattattgg gagctgcatc agctgctgta caatatccag
      241 atggaggagt atggacatat ggagaaggtt caggaggtgg ttaggctttt tcaaattact
      301 atcatggtaa aaaatatcat tattcatcca tcgtgagcag atgggatggt cattctgata
      361 aaggagaagc tcctgctgga aaaacctcct atgcatggat ttggactaaa tggggagaac
      421 aagtagcatt ttactgtgat tatgactaat cgttcattga tttaaatcgt tccccttgta
      481 agaaattaca gggggaattt tgtttacaag gagtctacta tgaagaaact attcatatta
      541 ttatcaactt tttttctcag cttcttcctt gcttggatta ttgtcttacg tgcgccacaa
      601 tatttatatg caagctatga ttccgtttct ttacttcgtg tcaaaaaaga tactcaggaa
      661 ccgacgcgtg aggtatttga acaggaattg gagaattttg caaactcaga acagagttta
      721 atagctagaa gaattgtaga gccgagtaag gatgggacga ctcactttac ttatgcaact
      781 tatggtcagg gaactttacc aaaagaattc caagaagcta gccaagaaag tcgtgaacgt
      841 agtgatccgc taaatagtta tctccttttg tcaggctcct tgacgaaaga aaagcttgcc
      901 gataaattag gagatttggg ttataaagca agtgctgacc gaaagatacc gccctatttt
      961 cttgcttttc gaatattact aaatcccctt attttaatta gtttagcaat atttggctta
     1021 tctttctttg ctttagtgat tatcactcgg attaaggaaa tgagagcagc aggtataaaa
     1081 ctcttttctg gtcagactct cttatccatc atggggcatt ctttatctac tgatatcaaa
     1141 tggctccttc tatcagccct cctttccttc ctaggtgggg gagtcgttct ttttagtcaa
     1201 ggtttgtttt atcctatctt gttagccacc tatggttttg ggattagttt ctatctgttg
     1261 tttttattgg cgatttcaat tttactaatg cttctttatc taatgagttt gaattacaaa
     1321 gcattagttc ccgttattaa ggggagatta ccccttaaac gcctgatgat tttaacccta
     1381 ttgtgtcagt tggttgctgt ctttacagta ggatacgctg ttaagacagg tttgacgtct
     1441 taccaacgat tgaaagaact tgaaatttca aaacaagcat ggcaggatag agcagactat
     1501 tatcaaattt cttttggctt aggtgataga ggaaaagata cagaaaatca gagcaagtgg
     1561 tatgcctttg ccaaggaagc aatcgaagaa gaacaagctc tttatgtaaa ggataatctg
     1621 ctccattttg ccaatccaca aggaaaaaat gaacagggag agacactgga tacctatagt
     1681 ccagatgcta atacgctcta tgttagtccc agttatttgg acaaggaaaa ggtcgtggta
     1741 gatgctgaga ccaaacagaa gttagcccat ctccaaaaag gtgagtttat cctcttgctc
     1801 ccagaacatt tgcgctctcg agaagcagaa cttaagaaag tttttgaaga aagattgagt
     1861 tattatggaa aatctggtga ggaggcaagt gctcctttgg attatgagat gaaagcgcac
     1921 gttagttatc tttcaatggg agaaaagcgg tttgtttata ataacggtga gaatcccgta
     1981 tccactcagt atttgactga tccgatttta gtggtattca cgccgacttc tacaggtgat
     2041 agttttatat ccctatctag ttggtctatc aatgctggaa aacaactctt tatcaaagga
     2101 tatgagagtg ggctagaact tttgaagaaa gctggaattt atgagcaagt atcctatctt
     2161 aaagaaggaa gaagtgttta tctaactcgt tataatgaag ttcaaactga aacagcaact
     2221 ttaatcttag gagctattgt ggggatagct agttccttgt tactatttta ttctgtcaat
     2281 cttctatatt tcgagcaatt ccgccgagat atcttgatta aacgaatttc aggtttacga
     2341 ttttttgaaa cacatgctca gtatatggtt agtcaatttg ccagttttgt atttggtgct
     2401 agtcttttta ttttaagcag tcgagacttg gtgattggct tgctcacttt attagtcttt
     2461 ctagctagtg cagttctgac gctttaccgt caagcgcaga aagaatctcg tgtttctatg
     2521 acaattatga aaggaaaata ggatgattga actaaagaat atatctaaaa aatttggaag
     2581 ccgtcagcta ttttcagata cgaatcttca ttttgaaggt gggaaaattt atgccttaat
     2641 cggtacaagt ggctgtggta agacaacact cttgaatatg attggacgat tagagccata
     2701 tgacaaaggg caaatcatct atgatggcac ttctcttaag gacatcaagc cttctgtttt
     2761 ctttagagat tacttaggat acttatttca agattttggc ttaattgaaa gccaaaccgt
     2821 caaagagaat ctcaatctgg gtttagttgg taaaaagttg aaggaaaaag agaaaatctc
     2881 tttgatgaaa caagctctaa accgtgtaaa cctctcttat ttggatttga agcaacctat
     2941 atttgagtta tcaggaggag aagcacaacg tgttgcatta gcgaagataa ttttaaagga
     3001 tccacctttg atcctcgcag atgaaccaac cgcttcctta gaccccaaaa actctgagga
     3061 attactttcc atcctagaat ctttaaaaaa tccgaatcga actattatta ttgcgaccca
     3121 caatcctctt atttgggaac aagtggacca agttattcga gttaccgatt tatcacatag
     3181 atgatatggc aagattcagc tagaagaaag agtcacaaac acactttgtg gcttttttat
     3241 ttccataaaa atggtaaaat agtaggagta gaaatggagt tcgagacatg aaagtaatag
     3301 atcaatttaa aaataagaaa gtccttgttt taggtttggc caagtctggt gaatctgcag
     3361 ctcgtttgtt ggacaagcta ggtgccattg tgacagtaaa tgatgggaag cctttcgagg
     3421 acaatccagc tgcccaaagt ttgctggaag aagggatcaa ggtcattaca ggtggccatc
     3481 ctttggaact cttggatgaa gagtttgctc ttatggtgaa aaatccaggt atcccctaca
     3541 acaatcccat gattgaaaag gctttggcca agggaattcc agtcttgact gaggtggaat
     3601 tggcttattt gatttcagaa gcaccgatta ttggtatcac aggatcgaac ggtaagacaa
     3661 ccacaacgac tatgattggg gaagttttga ctgctgctgg gcaacatggt cttttatcag
     3721 ggaatatcgg ctatccagct agtcaggttg ctcaaatagc gacagacaag aacacgcttg
     3781 ttatggaact ttcttctttc caactcatgg gtgttcaaga attccatcct gagattgcgg
     3841 ttattactaa ccttatgcca actcatatcg attatcatgg attatttgag gaatatgtag
     3901 cagccaagtg gaatatccag aacaagatga cagcagctga tttccttgtc ttgaacttta
     3961 atcaagactt ggcaaaagac ttggcttcca agacagaagc cactgttgta ccattttcaa
     4021 cacttgaaaa ggttgatgga gcttatctgg aagatggtca actctacttc cgtggtgaag
     4081 tagtcatggc agcgaatgaa atcggtgttc caggtagcca caatgtggaa aatgcccttg
     4141 cgactattgc tgtagccaag cttcgtgatg tggacaatca aaccatcaag gaaactcttt
     4201 cagccttcgg tggtgtcaaa caccgtctcc agtttgtgga tgacatcaag ggtgttaaat
     4261 tctataacga cagtaaatca actaatatct tggctactca aaaagcctta tcaggatttg
     4321 acaacagcaa ggtcgtcttg attgcaggtg gtttggaccg tggcaatgag tttgacgaat
     4381 tggtgccaga cattactgga ctcaagaaga tggtcatcct gggtcaatct gcagaacgtg
     4441 tcaaacgggc agcagacaag gctggtgtcg cttatgtgga ggcgacagat attgcagatg
     4501 cgacccgtaa ggcctatgaa cttgcgactc aaggagatgt ggttcttctt agtcctgcca
     4561 atgccagctg ggatatgtat gctaactttg aagtacgtgg cgacctcttt atcgacacag
     4621 tagcggagtt aaaagaataa aatatgaaaa aaattgtctt tacaggtggg gggacggttg
     4681 gacacgttac cctcaatctt ttgttaatgc ccaagttcat cgaagatggt tgggaagtcc
     4741 actatatcgg ggacaagcgt ggtatcgaac accaagaaat ccttaagtca ggtttggatg
     4801 tcaccttcca ttctattgcg actgggaaat tgcgtcgcta tttctcttgg caaaatatgc
     4861 tggacgtctt caaagttggc tggggaatcg tccaatcgct ctttatcatg ttgcgacttc
     4921 gtccacagac ccttttttca aagggaggct ttgtctcagt accgcctgtt atcgctgcgc
     4981 gtgtgtcagg agtgcctgtc tttattcacg aatctgacct gtctatgggc ttggccaata
     5041 aaatcgccta taaatttgcg actaagatgt attcaacctt tgaacaagct tcgagtttgg
     5101 ctaaggtcga gcatgtggga gcggtgacca aggtttcgga tcaaaaaaat ccagaaccag
     5161 atgaattggt ggatattcaa agccacttta atcataaatt gccgactgta ttgtttgttg
     5221 gcggttctgc aggtgctcgt gtctttaacc aattggtgac agaccataag aaagaactaa
     5281 cagagcgcta caatattatc aatctaactg gagattctag cctgaatgag ttgagccaaa
     5341 atctttttcg tgttgactat gtgaccgatc tctatcaacc cttgatggaa ttggctgata
     5401 ttgttgtgac acgtggtggt gccaatacga tttttgagct cttggcgata gcaaaattgc
     5461 atgtcattgt gccacttggt cgtgaagcta gtcgtggtga ccagattgaa aatgcagctt
     5521 actttgttaa gaaaggctat gcagaagacc ttcaagaaag cgatttgacc ttggatagtt
     5581 tggaagagaa gctttctcac ttactaagtc acaaggaaga ttaccaagct aagatgaaag
     5641 cttctaagga attgaaatct ctagcagatt tttatcaatt gttgaaaaaa gatttatcat
     5701 aaggaaagta aatgtcaaaa gataagaaaa atgaggacaa agaaatcctc gaagaattga
     5761 aagagttatc agaatggcag aaacgaaacc aagaatatct aaaaaagaag gctgaagaag
     5821 aagcggctct agctgaggag aaggaaaagg aaagacaagc tcggatggga gaagaatctg
     5881 agaagtcaga ggacaaacag gaccaggaga gtgaaacaga ccaggaagat tcagaatcag
     5941 ctaaggaaga gtctgaagaa aaagtagcat cctcagaggc tgacaaagag aaagaagaga
     6001 aagaagaatc agagtctaaa gagaaggagg aacaggataa aaagcttgct aaaaaggcta
     6061 caaaggaaaa accagccaaa gcaaagattc ctggtatcca tatcttgcga gccttcacga
     6121 ttttatttcc aagtctgctt ttattgattg tctctgccta cttgctcagt ccttatgcga
     6181 ccatgaaaga tattcgtgtt gagggaacgg tgcaaactac agctgatgat attcgacagg
     6241 cttcaggcat tcaggattcg gattatacga ttaaccttct gctagacaag gcaaaatatg
     6301 aaaagcagat taagtctaac tattgggttg aatcagctca acttgtctat caatttccaa
     6361 ctaagttcac tattaaggtc aaggaatatg atattgtggc ctactatatt tctggtgaaa
     6421 atcattatcc tattctttcc agtggtcagc ttgagactag ttctgtgagt ctgaacagtt
     6481 taccagaaac ttatttatca gttctcttta atgatagtga acaaatcaag gtttttgtct
     6541 cagaacttgc tcaaattagc ccagaactca aggcggctat ccaaaaggtg gaattagccc
     6601 caagcaaggt gacatccgat ttaattcgat tgaccatgaa tgattcggac gaagtcttgg
     6661 ttcctctatc tgaaatgagt aagaaactgc catattacag taagattaag ccacaattgt
     6721 cagaaccgag tgtggtcgac atggaagctg gaatttacag ttacactgtg gcggataaat
     6781 taattatgga ggctgaggaa aaagccaaac aagaggccaa ggaagctgag aaaaaacaag
     6841 aagaagaaca gaaaaaacaa gaggaagaga gcaatcgaaa tcaaacaaat cagcgttcat
     6901 cgcgtcgcta ggtttacctt ttctcttata gttctttagt gaccatgttt ttacgtttaa
     6961 tatttgacat ttgtttttct ttatgttaca tctgcaatgt aatcgattac aaaataattt
     7021 ttgatgaaga aggtaacaca tatgtcagat gaagtattct tatttgaaga aattgaagaa
     7081 atcgtagccc ctacagatgg tgaatttttg ggggaagttt tacttggaac tggggtagtt
     7141 ctcttaattg gagtagcctg ttgttaaaaa gatagggagt gataatcatg caagataact
     7201 ttttatttga ggaaattgaa gaaatttcag taccagttag tgatttttca gctggacttg
     7261 caacaggtat cggatttggt ttagcaatcc ttgctcttgc tggttgttga agtttgttca
     7321 tttactaaca tcaagctttt tcaatttcat tttagacagt catttgaatt ttccgtatta
     7381 gtcttgcagc aagagattaa tagaattagt cattatttta ttgattgcgg actgagggac
     7441 tagagtatgt tttacttagc ccctctttta tttattaaag gttaggtttg ttatgagaat
     7501 tgttgataag attaagatat tacctactcc ttatgaggga cagtatcatc tatatatacc
     7561 atccagtaag aaacatgtat tagttgggaa acaggaaaaa aatggttaga gcaactaata
     7621 ggtcaagaat ttaccatatc ggacttatta gtgttagtag ggaagaa
//
LOCUS       DAVLVZ010000081         7167 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000081 DAVLVZ010000000
VERSION     DAVLVZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.081"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            311..6745
                     /locus_tag="VYN77_001819"
     CDS             311..6745
                     /locus_tag="VYN77_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1559239.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     VVDKETENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDTKTSNEEAKIKE
                     ENSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKGLSNLKNTKVLYT
                     YDRIFNGSAIETTPDNLDKIKQIEGISSIERAQKVQPMMNHARKEIGVEEAIDYLKSI
                     NAPFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDK
                     IPHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNA
                     QIFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRA
                     LRKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQ
                     TVEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLD
                     LRGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEG
                     TKSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNV
                     GDEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVI
                     NGKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIAL
                     QNTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYG
                     SISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDG
                     KQIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFES
                     VEEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDG
                     KPKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSS
                     GSKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVI
                     SREHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPAT
                     KIRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLI
                     TKDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTY
                     AGEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFADQADEK
                     GMISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEG
                     EASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
BASE COUNT         2813 a          976 c         1376 g         2002 t
ORIGIN      
        1 cgtcagttcc atctacaacc tcaaaacagt gttttgagca acctgcggct agtttcctag
       61 tttgctcttt gattttcatt gagtactaat cttatttttt aacgtgtgct aaaaaataaa
      121 ttaatgcatg ttaatttttt tgacttaaat ttttttaaat gatatactgt ttttatagag
      181 agataacaat tatatataaa tgtactactc tatttcctag ataatactta gtaaaacttt
      241 ttataacaaa ggctagcaaa gttaaagttt attatctatt ggaagttaaa taaatatgta
      301 aggaattaaa atgaaaaaaa gcacagtatt gtcattaact acagctgcag ttattttagc
      361 agcctatgcc cctaatgagg tagtcttagc agacacatct agctctgaag atgctttaag
      421 catctctgat aaagaaaaag tagtagtaga taaggaaaca gaaaataaag agaaacataa
      481 agatattcat aatgctatag aaacttcaaa ggatactgaa gaaaagaaaa caacaattat
      541 tgaggaaaaa gaagttgtta gtaaaaatcc tgtaatagac actaaaacta gcaatgaaga
      601 agcaaaaatc aaagaagaaa attccaatca atcccaagga gatcatacgg actcatttgt
      661 gaataaaaac acagaaaatc ccaaaaaaga agataaagtt gtctatattg ctgaatttaa
      721 agataaagaa tctggagaaa aagcaatcaa gggactatca aatcttaaga atacaaaagt
      781 tttatatact tatgatagaa tttttaacgg tagtgccata gaaacaactc cagataactt
      841 ggacaaaatt aaacaaatag aaggtatttc atcgattgaa agggcacaaa aagtccaacc
      901 catgatgaat catgccagaa aggaaattgg agttgaggaa gctattgatt acctaaagtc
      961 tatcaatgct ccgtttggga aaaattttga tggtagaggt atggtcattt caaatatcga
     1021 tactggaaca gattatagac ataaggctat gagaatcgat gatgatgcca aagcctcaat
     1081 gagatttaaa aaagaagact taaaaggaac tgataaaaat tattggttga gtgataaaat
     1141 ccctcatgcg ttcaattatt ataatggtgg caaaatcact gtagaaaaat atgatgatgg
     1201 aagggattat tttgacccac atgggatgca tattgcaggg attcttgctg gaaatgatac
     1261 tgaacaagat atcaaaaact ttaacggcat agatggaatt gcacctaatg cacaaatttt
     1321 ctcttacaaa atgtattctg acgcaggatc tgggtttgcg ggtgatgaaa caatgtttca
     1381 tgctattgaa gattctatca aacacaacgt tgatgttgtt tcggtatcat ctggttttac
     1441 aggaacaggt cttgtaggtg agaaatattg gcaagctatt cgggcattaa gaaaagcagg
     1501 cattccaatg gttgtcgcta cgggtaacta tgcgacttct gcttcaagtt cttcatggga
     1561 tttagtagca aataatcatc tgaaaatgac cgacactgga aatgtaacac gaactgcagc
     1621 acatgaggat gcgatagcgg tcgcttctgc taaaaatcaa acagttgagt ttgataaagt
     1681 taacataggt ggagaaagtt ttaaatacag aaatataggg gcctttttcg ataagaataa
     1741 aatcacaaca aatgaagatg gaacaaaagc tcctagtaaa ttaaaatttg tatatatagg
     1801 caaggggcaa gaccaagatt tgataggttt ggatcttagg ggcaaaattg cagtaatgga
     1861 tagaatttat acaaaggatt taaaaaatgc ttttaaaaaa gctatggata agggtgcacg
     1921 cgccattatg gttgtaaata ctgtaaatta ctacaataga gataattgga cagagcttcc
     1981 agctatggga tatgaagcgg atgaaggtac taaaagtcaa gtgttttcaa tttcaggaga
     2041 tgatggtgta aagctatgga acatgattaa tcctgataaa aaaactgaag tcaaaagaaa
     2101 taataaagaa gattttaaag ataaattgga gcaatactat ccaattgata tggaaagttt
     2161 taattccaac aaaccgaatg taggtgacga aaaagagatt gactttaagt ttgcacctga
     2221 cacagacaaa gaactctata aagaagatat catcgttcca gcaggatcca catcttgggg
     2281 gccaagaata gatttacttt taaaacccga tgtttcagca cctggtaaaa atattaaatc
     2341 tacgcttaat gttattaatg gcaaatcaac ttatggctat atgtcaggaa ctagtatggc
     2401 gactccaatc gtggcagctt ctactgtttt gattagaccg aaattaaagg aaatgcttga
     2461 aagacctgta ttgaaaaatc ttaagggaga tgacaaaata gatcttacaa gtcttacaaa
     2521 aattgcccta caaaatactg ctcgacctat gatggatgca acttcttgga aagaaaaaag
     2581 tcaatacttt gcatcaccta gacaacaggg agcaggccta attaatgtgg ccaatgcttt
     2641 gagaaatgaa gttgtagcaa ctttcaaaaa cactgattct aaaggtttgg taaactcata
     2701 tggttccatt tctcttaaag aaataaaagg tgataaaaaa tactttacaa tcaagcttca
     2761 caatacatca aacagacctt tgacttttaa agtttcagca tcagcgataa ctacagattc
     2821 tctaactgac agattaaaac ttgatgaaac atataaagat gaaaaatctc cagatggtaa
     2881 gcaaattgtt ccagaaattc acccagaaaa agtcaaagga gcaaatatca catttgagca
     2941 tgatactttc actataggcg caaattctag ctttgatttg aatgcggtta taaatgttgg
     3001 agaggccaaa aacaaaaata aatttgtaga atcatttatt cattttgagt cagtggaaga
     3061 aatggaagct ctaaactcca acgggaagaa aataaacttc caaccttctt tgtcgatgcc
     3121 tctaatggga tttgctggga attggaacca cgaaccaatc cttgataaat gggcttggga
     3181 agaagggtca agatcaaaaa cactgggagg ttatgatgat gatggtaaac cgaaaattcc
     3241 aggaacctta aataagggaa ttggtggaga acatggtata gataaattta atccagcagg
     3301 agttatacaa aatagaaaag ataaaaatac aacatccctg gatcaaaatc cagaattatt
     3361 tgctttcaat aaccaaggga tcaacgctcc atcatcaagt ggttctaaga ttgctaacat
     3421 ttatccttta gattcaaatg gaaatcctca agatgctcaa cttgaaagag gattaacacc
     3481 ttctccactt gtattaagaa gtgcagaaga aggattgatt tcaatagtaa atacaaataa
     3541 agagggagaa aatcaaagag acttaaaagt catttcgaga gaacacttta ttagaggaat
     3601 tttaaattct aaaagcaatg atgcaaaggg aatcaaatca tctaaactaa aagtttgggg
     3661 tgacttgaag tgggatggac tcatctataa tcctagaggt agagaagaaa atgcaccaga
     3721 aagtaaggat aatcaagatc ctgctactaa gataagaggt caatttgaac cgattgcgga
     3781 aggtcaatat ttctataaat ttaaatatag attaactaaa gattacccat ggcaggtttc
     3841 ctatattcct gtaaaaattg ataacaccgc ccctaagatt gtttcggttg atttttcaaa
     3901 tcctgaaaaa attaagttga ttacaaagga tacttatcat aaggtaaaag atcagtataa
     3961 gaatgaaacg ctatttgcga gagatcaaaa agaacatcct gaaaaatttg acgagattgc
     4021 gaacgaagtt tggtatgctg gcgccgctct tgttaatgaa gatggagagg ttgaaaaaaa
     4081 tcttgaagta acttacgcag gtgagggtca aggaagaaat agaaaacttg acaaagacgg
     4141 aaataccatt tatgaaatta aaggtgcagg agatttaaga ggaaaaatca ttgaagtcat
     4201 tgcattagat ggttctagca atttcacaaa gattcataga attaaatttg ctgatcaggc
     4261 tgatgaaaag gggatgattt cttattacct agtagatcct gacaaagatg cttctaaata
     4321 tgaaaaactt ggtgaaattt ctgaagataa actcaaaaat gcaaagagcc cagaggaaaa
     4381 taccaataat aatcaagcta aggatgaaga ttcaaaacca gatgaaaaaa gttcagttga
     4441 gggagaagct agtcttgaaa taaataaaac tatttcaaca attagagagt ttgaaaataa
     4501 agacctaaag aaactcatta aaaagaaatt tagagaagtt gatgatttta caagtgaaac
     4561 tggtaagaga atagaggaat acgattataa atacgatgat aagggaaata ttattgctta
     4621 tgacgatggt agtgccttac aatatgaaac tgaaaaattt gacgaaataa aatcaaaaat
     4681 ttatggtgtt ctaagcccat ctaaagatgg acactttgaa attcttggaa agataagcaa
     4741 tgtttctaaa aatgccaagg tatattatgg aaatagctat aaatcgatag aaatcaaagc
     4801 gactaagtat gattcacatt caaaaacgat gatatttgat ttatacgcta atattaatga
     4861 tattgtagat ggattagctt ttgctggaga tatgagatta tttgttaaag atgacaatca
     4921 aataaaagct gaaactaaga ttagaatgcc tgaaaaaaat aaggaaacta aagcagaata
     4981 tccctatgta tcaagttatg ggaatgtaat cgaattagga gaaggagatc tttcaaagaa
     5041 caaaccagac aatttaacta aaatggaatc tggtaaaatc tattctgatt cagaaaaaca
     5101 acaatatctg ttaaaggata acatcattct aagaaaaggc tatgcactaa aagtgactac
     5161 ctataatcct ggaaaaacgg atatgttaga aggaaatgga gtctatagca aggaagatat
     5221 agcaaaaata caaaaggcca atcctaatct aagagtcctt tcagaaacaa caatttatgc
     5281 tgatagtaga aatgttgaag atggaagaag tacccaagct gtattaatgt cggctttgga
     5341 cggctttaac attataaggt atcaagtgtt tacatttaaa atgaacgata aaggggaagc
     5401 tatcgataaa gacggaaatc ttgtgacaga ttcttctaaa cttgtattat ttggtaagga
     5461 tgataaagaa tacactggag aggataagtc caatgtagaa gctataaaag aagatggctc
     5521 catgttattt attgatacca aaccagtaaa cctttcaatg gataagaact actttaatcc
     5581 atctaaatct aataaaattt atgtacgaaa tccagaattt tatttaagag gtaagatttc
     5641 tgataagggt ggttttaact gggaattgag agttaatgaa tcggttgtag ataattattt
     5701 aatctacgga gatttacaca ttgataacac tagagatttt aatattaagc tgaatgttaa
     5761 agacggtgac atcatggact ggggaatgaa agactataaa gcaaacggat ttccagataa
     5821 ggtaacagat atggatggaa atgtttatct tcaaactggc tatagcgatt tgaatgctaa
     5881 agcagttgga gtccactatc agtttttata tgataatgtt aaacctgaag taaacattga
     5941 tcctaaggga aatactagta tcgaatatgc tgatggaaaa tctgtagtct ttaacatcaa
     6001 tgataaaaga aataatggat tcgatggtga gattcaagaa caacatattt atgtaaatgg
     6061 aaaagaatat acatcatttg atgatattaa acaaataaca gacaagacac taaacattaa
     6121 gattgttgta aaagattttg caagaaatac aaccgtaaaa gaattcattt taaataaaga
     6181 tacgggagag gtaagtgaat taaaacctca tagggtaact gtgaccattc aaaatggaaa
     6241 agaaatgagt tcaacgatag tgtcggaaga agattttatt ttacctgttt ataagggtga
     6301 attagaaaaa ggataccaat ttgatggttg ggaaatttct ggtttcgaag gtaaaaaaga
     6361 cgctggctat gttattaatc tatcaaaaga tacctttata aaacctgtat tcaagaaaat
     6421 agaggagaaa aaggaggaag agaataaacc tacttttgat gtatcgaaaa agaaagataa
     6481 cccacaagta aatcatagtc aattaaatga aagtcacaga aaagaggatt tacaaagaga
     6541 agatcattca caaaaatctg attcaactaa ggatgttaca gctacagttc ttgataaaaa
     6601 caatatcagt agtaaatcaa ctactaacaa tcctaataag ttgccaaaaa ctggaacagc
     6661 aagcggagcc cagacactat tagctgccgg aataatgttt atagtaggaa tttttcttgg
     6721 attgaagaaa aaaaatcaag attaagacaa aagctataga aaaaaatggt ttatgtactg
     6781 agattagata gtgaggtgat gacatagttt tgtgaaaaca gccatttata actcaattat
     6841 ttagtttact tcactattga tactatttgg agttattaat ggacttagtt tagataacta
     6901 atgaattgat tgaaagggtt agtattgaca atattggtca tattgattag aaaatagagt
     6961 ctatcaaaat ttaaaggcta atagaggtga tgagacaatt tcaaggaatt aagtagcgtt
     7021 tggttgctta attccttttt atttggaagg aagtttttcg taaccgtcca ataacgaagt
     7081 ctattggacg gttacgatat gatcactgac ttcgtcagtc ttatctacaa cctcaaaact
     7141 gtgttttgag caacctgcgg ctagctt
//
LOCUS       DAVLVZ010000082         7092 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000082 DAVLVZ010000000
VERSION     DAVLVZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.082"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..670
                     /locus_tag="VYN77_001820"
                     /pseudo
     CDS             1..670
                     /locus_tag="VYN77_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            781..1707
                     /locus_tag="VYN77_001821"
     CDS             781..1707
                     /locus_tag="VYN77_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1559240.1"
                     /translation="MDLSQTMPSYAQVVSLYDDTSISVSNNETDKVLAGSLYTDTNEQ
                     GLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQFQTKIVRVINDE
                     DDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQKNKKYTVLS
                     QQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIGVMKAVGYQ
                     NRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFELNLLSVLG
                     TLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1884..2891
                     /gene="gap"
                     /locus_tag="VYN77_001822"
     CDS             1884..2891
                     /gene="gap"
                     /locus_tag="VYN77_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1559241.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2958..3794)
                     /locus_tag="VYN77_001823"
     CDS             complement(2958..3794)
                     /locus_tag="VYN77_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1559242.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3920..6115
                     /locus_tag="VYN77_001824"
     CDS             3920..6115
                     /locus_tag="VYN77_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1559243.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            6168..6320
                     /gene="rpmG"
                     /locus_tag="VYN77_001825"
     CDS             6168..6320
                     /gene="rpmG"
                     /locus_tag="VYN77_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1559244.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6330..6506
                     /gene="secE"
                     /locus_tag="VYN77_001826"
     CDS             6330..6506
                     /gene="secE"
                     /locus_tag="VYN77_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1559245.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6561..>7092
                     /gene="nusG"
                     /locus_tag="VYN77_001827"
     CDS             6561..>7092
                     /gene="nusG"
                     /locus_tag="VYN77_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1559246.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYALVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2215 a         1244 c         1525 g         2108 t
ORIGIN      
        1 gttgatagag atagacttaa aaactatctt actgacaatc cagacgctta tttgactgaa
       61 atagcttctg aatttggctg tcatccaact accatccact atgcgctcaa agctatgggc
      121 tacactcgaa aaaaagaacc acacctacta tgaacaagac ccagaaaaag tagccttatt
      181 tcttaaaaat tttaatagtt taaagcacct agcacctgtt tagattgatg aaacaggatt
      241 cgatacttat ttttatcgag aatatggtcg ctcattaaaa ggtcagttaa gaagaggtaa
      301 agtatctgga agaagatatc agaggatttc tttggttgca ggtcgaacaa atagtgagtt
      361 aatcgctcca atgacttacg aagagatgat gacgagcgac ttttttgaag cttggtttca
      421 gaagtttctc ttaccaacat taaccacacc atcggttatt attatggata atgcaagatt
      481 ccatagaatg ggtaagttag aactcttgtg tgaagagttt gggcataaac ttttacctct
      541 tcctccctac tcgcctgagt acaatcctat tgagaaaaca tgggctcata tcaaaaagca
      601 cctcaaaaag gtattaccaa gttgcaatac cttttatgag gctcttttgt cctgctcttg
      661 tttcaattga ctatagttca cggatacagt tgggaaagaa gctattaaat atttggagag
      721 tccttttggg atgaacatgt caaatattac tatagatggg caagaaaaga tgggcaagaa
      781 atggatctga gtcaaacgat gccttcttat gctcaagtag tgagtcttta tgatgataca
      841 agtatctctg ttagtaataa tgagacggac aaagtgttgg ctggctccct ctatactgat
      901 acaaatgaac agggattaac gattccaagc agtttactaa aaaattggaa tgaacagaca
      961 ggaaaaaatt tgacagctaa tgatcttatt ggcaaatcag tctcagccag cattgtagaa
     1021 agtgctgccg aaactagtaa gattgctcaa tttcaaacga agattgtacg tgtaatcaat
     1081 gatgaagatg acatggagga cagcaacagt ttcatgctgt ctcatcaaat ggaaacgatt
     1141 ttgaaagagg ctggatttac gaaagctgta tcttatttta tcttggaact caaagatcca
     1201 tcacagacaa aagtagtaac agaagaatta cagaaaaata agaagtatac tgtgctttct
     1261 caacagagag ttcttgatat tgtgattacc tttattcgtg ttattcaggg attattgatt
     1321 gtgctttcat cacaagctat tgtggtagca gcggttatga ttggtatcat tatttacatc
     1381 aatatcatgc aacgttccaa ggaaataggt gtcatgaaag cagttggtta tcagaatcgt
     1441 ggtgtcaaag gaatttttat ttacgaggct atctggattg taggcatcgc cttgctgctg
     1501 gcatttttgg ttgcacaagg ggtgggaagt ttggcgaatg cgattgtaag tcacttttac
     1561 ccatccatca ctaaggtttt tgaattaaat cttttatctg ttttaggaac tctagttttc
     1621 gctctattac ttggttatgt ctcagcctac ttcccggcgc gtaagattag taaaatggat
     1681 cctgtagaat cgttacgcta tgaataataa gggattcctt gatttacagt cttaaaaaga
     1741 aactcacata gaaaaacaat ggccatactt gcaattcaac tcaaatagtt gtataatagg
     1801 tttgttgaaa ggtgatttgt agtgattcaa gttactcttt tcacaataaa aatttcatga
     1861 ttttcataag gaggaaatca ctaatggtag ttaaagttgg tattaacggt ttcggacgta
     1921 tcggtcgtct tgctttccgt cgtatccaaa acgtagaagg tgttgaagtt acacgcatca
     1981 acgaccttac agatccagtt atgcttgcac acttgttgaa atacgacaca actcaaggtc
     2041 gtttcgacgg tactgttgaa gttaaagaag gtggattcga agttaacggt aaattcgtta
     2101 aagtttctgc tgaacgtgat ccagagcaaa tcgactgggc tactgacggt gtagaaatcg
     2161 ttcttgaagc tactggtttc tttgctaaga aagaagcagc tgaaaaacac cttaaaggtg
     2221 gagctaaaaa agttgttatc actgctcctg gtggaaacga cgttaaaaca gttgtattca
     2281 acactaacca cgacgttctt gacggtactg aaacagttat ctcaggtgct tcatgtacta
     2341 caaactgctt ggctccaatg gctaaagctc ttcaagataa ctttggtgtt gttgaaggat
     2401 tgatgactac tatccacgct tacactggtg accaaatgat ccttgacgga ccacaccgtg
     2461 gtggtgacct tcgccgtgct cgcgctggtg ctgcaaacat cgttcctaac tcaactggtg
     2521 ctgctaaagc tatcggtctt gtaatcccag aattgaacgg taaacttgac ggatctgcac
     2581 aacgcgttcc aactccaact ggatcagtta ctgaattggt agcagttctt gaaaagaacg
     2641 ttactgttga tgaagtgaac gcagctatga aagcagcttc aaacgaatca tacggttaca
     2701 cagaagatcc aatcgtatct tcagatatcg taggtatgtc ttacggttca ttgtttgacg
     2761 caactcaaac taaagttctt gacgttgacg gtaaacaatt ggttaaagtt gtatcatggt
     2821 acgacaacga aatgtcatac actgcacaac ttgttcgtac tcttgaatac ttcgcaaaaa
     2881 ttgctaaata attcttgagt tgatagaaag caaggctttg tggtcttgct ttttatatgg
     2941 aaaaatggat gacacgatca tccattcttt tttaattctt tttcaaatgt atttgaaagg
     3001 gtagtgaaag ttagcttctc taaagtaagt gggtgggtaa aggaaagtcg gaaggcatga
     3061 agcataagcc gacttgtctt tgatttacta ttatagagag ggtcgcccag gataggaaga
     3121 ttatgatgcg aaaggtgcac acgaatctga tgggttcgcc ctgtctttag cttgcaacga
     3181 gccaaggaag tcttgtttga gaattgcttt aatctgctta catgcgtttc agcatattgc
     3241 ccattttttg catcaactat tctttttcta cgatcatggc gatcacgtcc aattttgtct
     3301 ctgaaaacaa gttcttttct gttgatattt ccatcaacta gagcccaata ttctctagaa
     3361 atctcttttt tctccaataa gcgattgaga atgggcagga taaaaggatt tttggcaaag
     3421 agaactaagc cactggtttc catgtccaga cgatgaacga catagcaggt ttggccaaca
     3481 taggtactga catggttaag aagggcaatt tcgtttggtt gattaccatg cgttttcatc
     3541 ccctctggtt tgtttacaat aatcaagtgt tgatcttgat aaacttcctg cactaagtct
     3601 gggttgcccc aagggatcgt cttttgggaa taatcttcct cgtcaaaagt caactggcaa
     3661 acatctccag gatttacgat ttcgttccag tggacttctt cttgatttat caaaatatgt
     3721 ttcttgattc tcaaaaaatg acggattttt ctagggatga ggagttgttc ctcaagtaat
     3781 tgctttaccg tcatttgagg tagagaggcg ggtaatgtaa atgtgaattg catacagata
     3841 ttgtaacaaa aaaagcccta tttggatagg aaatagctaa attcttgtct tcctatgatg
     3901 aagatgataa aataaacgca tgaaattaga taaattattt gagaaatttc tttctctttt
     3961 taaaaaagaa acaagtgaac tagaggactc tgattctact atcttacgtc gctctcgtag
     4021 tgatcgaaaa aaattagccc aagtaggtcc gattcgaaaa ttctggcgtc gttatcatct
     4081 aacaaagatt atccttatac taggtttgag tgcaggattg ctagttggaa tctatttgtt
     4141 tgctgtagcc aagtcgacca atgtcaatga tttgcaaaat gccttgaaaa ctcggactct
     4201 tatttttgac cgtgaagaaa aagaggctgg tgccttgtct ggtcaaaagg gaacctatgt
     4261 tgagctgact gacatcagta aaaacttgca gaatgctgtt attgcgacag aagaccgttc
     4321 tttctataaa aatgacggga ttaactatgg ccgtttcttc ttggctattg tcactgctgg
     4381 acgttcaggt ggtggctcta ccattaccca acagctggct aaaaacgcct atttatcgca
     4441 ggatcaaact gttgagagaa aagcgaaaga atttttcctt gccttagaat taagcaaaaa
     4501 atatagtaag gagcaaattc taaccatgta ccttaacaat gcttattttg gaaatggtgt
     4561 gtggggtgta gaagatgcga gtaagaaata ctttggagtt tctgcatcag aagtgagtct
     4621 ggatcaagct gcgactctgg cagggatgct caaggggccg gaactgtata atcccttgaa
     4681 ttctgtagaa gattctacta atcggcgcga tactgtcttg cagaatatgg ttgcagcagg
     4741 atatattgat aaaaaccaag aaaccgaagc tgctgaagtt gatatgactt cgcaattgca
     4801 cgataagtat gaaggaaaaa tctcagatta ccgttacccc tcttattttg atgcggtggt
     4861 taatgaagct gtttccaagt ataatctaac agaggaagag attgtcaata atggctaccg
     4921 catttacaca gagctggacc aaaactacca agcaaatatg cagattgttt atgaaaacat
     4981 atcgctattt ccgagggcag aggatggaac gtttgctcaa tcaggaagtg tagctctcga
     5041 accgaaaaca gggggagttc gtggagttgt cggtcaagtt gctgacaatg ataaaactgg
     5101 attccggaat ttcaactatg caacccaatc aaagcgtagt cctggttcta caattaagcc
     5161 tttagttgtt tatacgccag cagttgaagc aggctgggct ttgaataagc agttggataa
     5221 ccataccatg cagtatgaca gctataaggt tgataactat gcagggatca aaacgagtcg
     5281 agaagttcct atgtatcaag ccttggcaga atcgcttaat ctacctgctg ttgccactgt
     5341 taatgatttg ggtgtcgaca aggcttttga ggcaggcgaa aaattcggac tcaacatgga
     5401 aaaggttgac cgtgttcttg gtgtcgcctt gggaagcggt gttgaaacca accctcttca
     5461 aatggctcaa gcatacgccg cctttgcaaa tgaaggttta atgcctgaag ctcattttat
     5521 tagtagaatt gaaaatgcta gtggacaagt tattgcgagt cataaaaatt cacaaaaacg
     5581 ggtgattgat aagtctgtag ctgacaagat gaccagtatg atgttgggga ctttcaccaa
     5641 cggtaccggt attagttcat cgcctgcaga ctatattatg gcagggaaaa ctggaacaac
     5701 tgaagcagtt tttaatccgg agtacacaag tgaccagtgg gtaattggtt atactccgga
     5761 tgtagtgatt agccactggc ttggctttcc gaccactgat gaaaaccact atctagctgg
     5821 ctctacttca aacggtgcag ctcatgtctt tagaaacatt gccaatacca ttttacctta
     5881 tacgtcagga agtaccttta cggttgaaaa tgcttataag caaaatggaa ttgcaccagc
     5941 caatacaaaa agacaagtac aaaccaatga taatagccag acagatgata atttgtctga
     6001 tattcgaggg cgtgcgcaaa gtctagtaga tgaggctagc cgggctatct cagatgcgaa
     6061 gattaaggaa aaggctcaaa caatatggga ttcgatagtc aatctatttc gctaagatgc
     6121 ttgtcaaagc ctagctttct tgttataatg gataagatgg aggcgttatg gcactaaaaa
     6181 aagcaagcct agcttgtgcg gtttgtggtt cgagaaacta ttcaatcaag atcagcggaa
     6241 accccaagcc tacacgacta gaagtaaata aattttgtaa gcattgtggc aagtacacta
     6301 cacatagaga aacgagatag gagagagcga tgcgttttat tggagatatt tttagacttc
     6361 ttaaagacac aacgtggcca actcgcaagg aaagctggag agattttcgt tctatcatgg
     6421 aatacacagc tttctttgta gtaattattt acatttttga ccagttgatt gtttcaggtt
     6481 tgattcgatt tattaacatt ttttagaaga ttagtggagt taattacact agaaatcttc
     6541 tatttatgaa aggaaatatc atggatagtt ttgataaagg atggtttgtt ttacaaactt
     6601 attctggtta tgaaaataag gtgaaagaaa atctattaca acgtgcacaa acctacaata
     6661 tgttggataa tattctacgc gttgaaattc caacacaaac agtgcaagtt gaaaaaaatg
     6721 gaaagagaaa agaagtagaa gaaaatcgct ttccaggtta tgctcttgta gaaatggtca
     6781 tgacagatga agcttggttt gttgttcgaa acacaccaaa tgttacagga tttgtcggat
     6841 cacacgggaa cagatcaaaa ccaactccat tattggaaca agaaattcgt gacattttgg
     6901 tatctatggg acaaactgtt caagaatttg atttcgatgt tgagattggt caaactgtac
     6961 gtattattga tggtgctttt gcagactaca ctggtaagat tacagaaatt gataataaca
     7021 aagtgaaaat gattatctct atgtttggta atgacacagt tgcagaagta aacctaaacc
     7081 aaatcgcaga at
//
LOCUS       DAVLVZ010000083         6659 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000083 DAVLVZ010000000
VERSION     DAVLVZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.083"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..425
                     /locus_tag="VYN77_001828"
                     /pseudo
     CDS             1..425
                     /locus_tag="VYN77_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            580..780
                     /locus_tag="VYN77_001829"
     CDS             580..780
                     /locus_tag="VYN77_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559247.1"
                     /translation="MVVSASASSTSPQAQEQVDKSELRALSQELDQHLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            805..1221
                     /locus_tag="VYN77_001830"
     CDS             805..1221
                     /locus_tag="VYN77_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559248.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1452..2159
                     /gene="ccdA2"
                     /locus_tag="VYN77_001831"
     CDS             1452..2159
                     /gene="ccdA2"
                     /locus_tag="VYN77_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1559249.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2170..2727
                     /gene="sdbB"
                     /locus_tag="VYN77_001832"
     CDS             2170..2727
                     /gene="sdbB"
                     /locus_tag="VYN77_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1559250.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3102..4493
                     /locus_tag="VYN77_001833"
     CDS             3102..4493
                     /locus_tag="VYN77_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1559251.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4636..5553
                     /gene="adcAII"
                     /locus_tag="VYN77_001834"
     CDS             4636..5553
                     /gene="adcAII"
                     /locus_tag="VYN77_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1559252.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDSDVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLDRVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTENNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5561..>6659
                     /gene="phtB"
                     /locus_tag="VYN77_001835"
     CDS             5561..>6659
                     /gene="phtB"
                     /locus_tag="VYN77_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV1559253.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPS"
BASE COUNT         2049 a         1220 c         1393 g         1997 t
ORIGIN      
        1 gactgaaata gcttctgaat ttggctgtca tccaactacc atccactatg cgctcaaagc
       61 tatgggctac actcgaaaaa aagaaccaca cctactatga acaagagcca gaaaaagtag
      121 ccttatttct taagaatttt aatagtttaa agcacctagc acctgtttag attgacgaaa
      181 caggattcga tacttatttt tatcgagaat atggtcgctc attaaaaggt cagttaataa
      241 gaggtaaagt atctggaaga agatatcaga ggatttcttt ggttgcaggt ctaacaaata
      301 gtgagttaat cgctccaatg acttacgaag agatgatgac gagcgacttt tttgaagctt
      361 ggtttcagaa gtttctctta ccaacattaa ccacaccatc ggttattata gtaaaacgaa
      421 ataagaatag gacaaatcga tcaggacagt aaaatcgaat tctaacaatg ttttagaagt
      481 ccatatgtac tattctagtt tcaatatacc ataactgctc ctagtcaacc tgcaagtcct
      541 gtcccagctc caagctcagc tccgactcaa gatcctagag tggtagtaag cgcttcggct
      601 agctcaacga gcccacaagc tcaagagcaa gttgacaagt ctgaacttcg tgccttgagt
      661 caagagttag accaacactt gaaagctttg gcaacagtat ctgatccgaa aatcgatgca
      721 accaaggctg tcctcctaga tgctcaaaaa gctccggaag atagtgcctt gacagagtaa
      781 ggtctgcata ctgcagtaga gtctgtgaag gctgccttgg actctctcaa agatgtgaaa
      841 gcaaatgcta gcgacagcaa gcctgcacag gacaagaagg atgcaaaaca aggaacggaa
      901 gatagtaagg attcagataa gatgactgaa acaaactcag ttccggcagg agtgattgtg
      961 gtcagtctac ttgccctcct aggcgtgatt gccttctggc tgattcgccg taagaaagag
     1021 tcagaaatcc agcaattaag cacggaatta atcaaggttc taggacagct agatgcagaa
     1081 aaagcggata aaaaagtcct tgccaaagcc caaaaccttc tccaagaaac ccttgatttc
     1141 gtgaaagaag aaaatggctc agcagagaca gaaactaaac tagtagagga gcttaaagca
     1201 atccttgaca aactcaagta aaacatgatt tatgaaatgt cagacttaaa agacattttc
     1261 gttcacaaaa gcagcgacga gtaatccttg tcgttgcttt ttggctattt tgggggataa
     1321 gatccgcctt gactagcaaa agaaaagatt ggtccaatag tgcccgatac gattttgcgt
     1381 agcagaaagc ttacaaataa aattgaatat agtaaaatag gattagaatt attaagaaag
     1441 ttggttcttt attggaaacg atagtatttt taatctctgt ttttctagca ggtgttttat
     1501 cctttttttc tccttgtatt tttcctcttc tgccagtcta tgctgggatt ttattggatg
     1561 atcaagaaag tgcaaaaagc ttttctttgt ttgggagaaa ggttctctgg tcaggcttga
     1621 ttcgaacact ttgctttatc gctggtatct ctctcatttt ctttattcta ggctttggtg
     1681 ctggttactt tggtcatatt ctctatgcaa attggtttcg atatggcatg ggagctatta
     1741 ttatcatttt gggtcttcat cagatggaaa tttttcattt gaagaaatta gaagttcaaa
     1801 aaagttttac ctttaaaaaa tcagattcta atcgttattg gtcagctttt ttacttggta
     1861 ttacctttag ctttggttgg acaccttgta ttggtccagt tttaagttct gttttagcac
     1921 ttgcggcttc tggaggcaat ggcgcttggc aaggagcgat ttatactctc atttacactc
     1981 tgggcatggc ccttcctttc ttggtattgg cactagcttc aggtctagtc atgccatatt
     2041 ttagtaaaat caagcgtcat atgatgctac taaagaaaat tggtggtttc ctcattgttt
     2101 taatgggaat tttgttacta ttaggacaag taaatgttct agctggaatt tttgaataaa
     2161 ggaggacaaa tgaaaaaagt aatgtttgct ggcttaagtc tcttgtcatt agttgtattg
     2221 atggcctgtg gtgaggaaga aactaaaaag actcaagcag cacaacagcc aaaacaacaa
     2281 acgactgtac aacaaattgc tgttggaaaa gatgctccag acttcacatt gcaatccatg
     2341 gatggcaaag aagttaagtt atctgatttt aagggtaaaa aggtttactt gaagttttgg
     2401 gcttcatggt gtggtccatg caagaaaagt atgccagagt tgatggaact agcggcgaaa
     2461 ccagatcgtg atttcgaaat tcttactgtc attgcaccag gaattcaagg tgaaaaaact
     2521 gttgagcaat tcccacaatg gttccaggaa caaggatata aggatatccc agttctttat
     2581 gataccaaag caaccacctt ccaagcttat caaattcgaa gcattcctac agaatattta
     2641 attgatagcc aaggaaagat tggaaagatt caatttggtg ctatcagtaa tgcggatgca
     2701 gaagcagcat ttaaagaaat gaactagaat cgataaaatc tgattacaag atgtagtcag
     2761 attttggtaa cttctaaaac taacttctag tttcccacaa cctagctttt agtatgagaa
     2821 aaacgtgtga caccagtgga aatccgtctt agactaactt ccactggttt tctttttctt
     2881 gatatataag ggttcaaaag cgaaaagact cagaaaaaag tgcacgacaa atagccctaa
     2941 aacagagtcg ttttagagta acttccagtt ttgggagaga gatggaagtt actttgagaa
     3001 gttacggtag tcagattttt ttagaaaaac attttataaa tattcacata tctcctatat
     3061 ttatggtaaa atagaatcat cagtttattt tggagtcaaa gatgaatata tttagaacaa
     3121 agaatgttag tttagataaa acagagatgc ataggcattt gaagttatgg gatttgattt
     3181 tgctgggtat tggagccatg gtagggacag gtgtctttac aatcacaggt actgcagctg
     3241 ctacacttgc tggtccagca ctagtgattt caatcgttat ttctgccttg tgtgtgggat
     3301 tatcagccct cttttttgca gaatttgcct cgcgagtacc cgctacagga ggtgcctata
     3361 gttacctcta tgctatctta ggagaattcc ctgcctggtt ggctggttgg ttaaccatga
     3421 tggagttcat gacagccata tcaggcgtag cttcgggttg ggcagcttat tttaaaggct
     3481 tgctctctca atacgggata gcccttcctc aggctttaaa tggtaccttt aatcctcaag
     3541 cagggacatt tgttgattta ttgcctattc ttgtcttggt cttggtgact tcgcttgttt
     3601 tactaaatgc caaggcagcc ttacgcttta attcgattct agttattttg aaattttccg
     3661 ctttagctct ctttgtttta gtaggaattt ggaatattaa gtttgataat tggagcaatt
     3721 ttgctcctta cggttttgga caaatctatg gagctagtac tggtattatg gctggtgcgt
     3781 ccttgatgtt cttcggtttt ttgggatttg aatccatctc tatggctgta gatgaagtca
     3841 agactcctca aaaaaatatt cctagaggga ttgtcttatc gctttctatc gtaaccattc
     3901 tctatgcctt ggtgaccctt gttttgactg gtgtggttca ctatagtcat ttaaatgtcg
     3961 atgatgccgt tgcctttgcc cttcgtagtg ttgggattag ttgggcagcc aactatgtgt
     4021 cattagtggc tatcttgacc ttgattacag tttgtatttc gatgacctat gccctatcgc
     4081 gtatgattta cagtttagca cgtgacggct tagtgcctgc tgcctttaag gaactgacga
     4141 agactagcaa ggtaccaaag aatgctacta tcttgacagg tctagcttca gcagtagcag
     4201 caggaatgtt cccactagcc agtattgcag cctttttaaa tatttgtacc ttagcctact
     4261 tgatcatgct ggcttatggt ctgattcgct tacggaaaga aaaaggaatg cccaaagctg
     4321 gggaatttaa aacaccattg gtacctctgt taccaatttt atcgatcatc atttgtttat
     4381 ccttcatgtt acagtataat atgaacacct ggattgcttt cctagttgcg ttgttagtag
     4441 gaagtattat ttacttcact tatggctata agcattctac catagaagaa taaagtgaaa
     4501 gtctattgag ttgttgacac tcagtagatt tttatatgtg aaagaaattt caaatttttt
     4561 ctaaaaatat cttgacagat taaattttca ggagtagaat atttactagt taattaaatg
     4621 gttaaggagt tgttcatgaa gaaacaaaat ttatttttag tcctgttaag tgtctttctt
     4681 ttgtgcttgg gggcttgtgg tcaaaaggaa agtcagacag gaaaggggat gaaaattgtg
     4741 accagttttt atcctatcta cgctatggtt aaggaagtat ctggtgactt gaatgatgtt
     4801 cggatgattc agtcaagtag tggtattcac tcctttgaac cttcggcaaa tgatatcgca
     4861 gccatctatg attcagatgt ctttgtttac cattctcata cactcgaatc ttgggcagga
     4921 agtctggatc caaatctaaa aaaatctaaa gtgaaggttt tagaggcttc agagggaatg
     4981 actttagacc gcgtccctgg actagaggat gtggaagcag gggatggagt tgatgaaaaa
     5041 acgctctatg accctcacac atggctagat cctgaaaaag ctggagaaga agcccaaatt
     5101 atcgctgata aactttcaga ggtggatagt gagcataaag agacttatca aaaaaatgcg
     5161 caagccttta tcaaaaaagc tcaggaattg actaagaaat tccaaccaaa atttgaaaaa
     5221 gcgactcaga aaacatttgt aacacaacac acagcctttt cttatctagc gaagagattt
     5281 gggcttaatc aacttggtat tgcaggtatc tctcctgaac aagaaccaag tccacgacaa
     5341 ctaacagaaa ttcaggaatt tgttaagacc tataaggtta aaacgatttt tacagaaaat
     5401 aacgcttctt caaaagtagc tgaaactctt gtcaaatcaa caggtgtggg tcttaaaact
     5461 ctgaatcctt tagagtcaga cccacaaaat gacaagacct atttagaaaa tcttgaagaa
     5521 aatatgagta ttctagcaga agaattaaag tgaggaaaga atgaaaatca ataaaaaata
     5581 tctagcaggt tcagtggcag tccttgccct aagtgtttgt tcctatgaac ttggtcgtca
     5641 ccaagctggt caggttaaga aagagtctaa tcgagttgct tatatagatg gtgatcaggc
     5701 tggtcaaaag gcagaaaatt tgacaccaga tgaagtcagt aagagagagg ggatcaacgc
     5761 cgaacaaatt gttatcaaga ttacggatca aggttatgtg acctctcatg gagaccatta
     5821 tcattactat aatggcaagg ttccttatga tgccatcatc agtgaagaac ttctcatgaa
     5881 agatccgaat tatcagttga aggattcaga cattgtcaat gaaatcaagg gtggctatgt
     5941 cattaaggta aacggtaaat actatgttta ccttaaggat gcagctcatg cggataatat
     6001 tcggacaaaa gaagagatta aacgtcagaa gcaggaacgc agtcataatc ataactcaag
     6061 agcagataat gctgttgctg cagccagagc ccaaggacgt tatacaacgg atgatgggta
     6121 tatcttcaat gcatctgata tcattgagga cacgggtgat gcttatatcg ttcctcacgg
     6181 cgaccattac cattacattc ctaagaatga gttatcagct agcgagttag ctgctgcaga
     6241 agcctattgg aatgggaagc agggatctcg tccttcttca agttctagtc ataatgcaaa
     6301 tccagctcaa ccaagattgt cagagaacca caatctgact gtcactccaa cttatcatca
     6361 aaatcaaggg gaaaacattt caagcctttt acgtgaattg tatgctaaac ccttatcaga
     6421 acgccatgtg gaatctgatg gccttatttt cgacccagcg caaatcacaa gtcgaaccgc
     6481 cagaggtgta gctgtccctc atggtaacca ttaccacttt atcccttatg aacaaatgtc
     6541 tgaattggaa aaacgaattg ctcgtattat tccccttcgt tatcgttcaa accattgggt
     6601 accagattca agaccagaac aaccaagtcc acaaccgact ccggaaccta gtccaagtc
//
LOCUS       DAVLVZ010000084         6326 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000084 DAVLVZ010000000
VERSION     DAVLVZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.084"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(11..1234)
                     /locus_tag="VYN77_001836"
     CDS             complement(11..1234)
                     /locus_tag="VYN77_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1559254.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            1402..2724
                     /locus_tag="VYN77_001837"
     CDS             1402..2724
                     /locus_tag="VYN77_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1559255.1"
                     /translation="MNNILTFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VTAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPSIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            2726..4612
                     /locus_tag="VYN77_001838"
     CDS             2726..4612
                     /locus_tag="VYN77_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEV1559256.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGHRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHIWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWVPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFFALYGENWQSVIEYLEKL
                     SIYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            4780..5124
                     /locus_tag="VYN77_001839"
     CDS             4780..5124
                     /locus_tag="VYN77_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1559257.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            5134..>6326
                     /locus_tag="VYN77_001840"
     CDS             5134..>6326
                     /locus_tag="VYN77_001840"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1559258.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHDLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGEIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDD"
BASE COUNT         1984 a          908 c         1301 g         2133 t
ORIGIN      
        1 ttctaatata ttaaggtgaa ataatatctt gtaatacatt gctatgttct atgaaaaaag
       61 cgacgatagc taatgcacaa gctcctatac ttccacgatg tttgttgtaa ggtaaaattt
      121 caatttctat attacgagta aagggaacga agtggagctg gtcttggatt ttatccctga
      181 cttcatgagt gaaaggttga taattaagca attgactgtt gatatagatt ttatcagcag
      241 gattgatgag gaggagattt gcaatagaag tggctaataa attgactcct ttatcaattt
      301 gttgacgtaa agcggagtcg cctaaattat aagccgttaa aagggtgtct aaatgaatgt
      361 ctttttcagt ctttacaagg ctttttagta cagttagttg ggaattttta aataataatt
      421 gggcgtgttt gattaaccaa gcatccgaaa tatatgtttg taaacaacct ttttttccgc
      481 attcacaata ttgcccattc aattcgacaa tggtgtgtcc aatttctccg atataaggat
      541 ttttagaggc tcctattttt tcttttgtga aaaaggaagt gtaaatacct aatccagcgt
      601 gtaggaaaat aaagttatcg ggggtattgt gtggattaaa aaggtattgt cctatagcca
      661 tacaatcgat attatttttt acaataaaag gaaaattgaa tcttttaatt acatttaata
      721 aattaaaaga ttcccatatg gggttgtttg taatgatact tccggagtct ttgttataat
      781 gtccaggtat tgataagcca aatccaagta attttgtttc caaagggacg tactctatag
      841 ctgtttttat catattggta atggtggaat tgatttctga aataggataa tcttcattca
      901 aaatttctgt ctcgaaaaga gttttccctt gtaagtcaca tagactgaga aagataccta
      961 ggacgtttat ttcaatacct ccaacaacat acggaatatt atctgtgata gaaatagttt
     1021 ttctacgtct tccagatccg gaaaactcgc taaattcatc accagtttcg tatatgaggg
     1081 actgttcgct tagctctttt atgatgttag tcgttgttgc tggagtaagt cctgtttcat
     1141 taacaatatc tgtacgagat gttgctggat ttcgatatag aaactccagt atagtggctc
     1201 tattttgtaa aaaggctgat tgttttgtaa tcatttgttt cctcctttgg tttattttat
     1261 attaaacata agaaaaaatc aattaaaatt attattctat acaaagaaaa aaatattgta
     1321 aaaacagcaa aaaatatatt gacaacgctt tctattagtg gtatatttaa tttataaaaa
     1381 ataaaaaaag gagttccggt gatgaacaat attttaacgt ttttagaaac aaaagtcgct
     1441 ccgtttggtg aaaaagttgg caaccaacgg catttgaaag ctattcgtga aggatttatg
     1501 atggcaatgc ctttaatttt agtcggctct ttatttctta ttctaatcag ttggcctcaa
     1561 gaggatttta caaattggct gaatagtgtt ggattgctaa gtatcttgac aactatgaat
     1621 cagtcaacag tggcgattat ctccttggtc gcttgtttcg gtattgccta caggttgtcg
     1681 gaaggatatg gtacagatgg tccgtcggca gggatcatag ccttatccag ttttgtattg
     1741 atggcacctc gtttttcgag tatggtttat gataaaaatg gggagcaggt caagcagtta
     1801 tttggcggcg caataccatt ttctagcctg aatgcatctt ctttgtttat ggcgattact
     1861 attggattgg ttacagcaga gatttatcgt atgtttatcc agcgcggaat tacgataaaa
     1921 atgccaagtg gtgtcccaga tgtggtaagt aaatcatttt cagctctttt acctggcttt
     1981 actacttttg ttttgtgggc tctggtctta aaaggtcttg aagcggcagg agttgcagga
     2041 ggtctcaacg gactcctagg tgcaattgtt ggaacaccgc ttaagttaat tgcaggaacg
     2101 cttccaggta tgattctatg tgttattgta aattcattct tttggttctg tggagttaat
     2161 gggggacaag ttttaaatgc ttttgtagac ccagtttggt tacaatttac tacagaaaac
     2221 caagaagctg tgactgcagg acaaacactc caacacatta ttacattacc gtttaaagat
     2281 ttatttgtat ttattggtgg cggtggagcg actattggtc ttgcgatttg tctcttccta
     2341 tttagtaaga gtcgtgcgaa taaaacatta ggtaagctag ctattatacc gtctattttt
     2401 aatatcaata cagctattct atttacgttt ccaacagttt taaatccgat tatgctgatt
     2461 ccgtctattg ctactcctac aatcaatgcc ttgattacct atgtatcaat ggctgtagga
     2521 ttagtaccct atacaacagg tgtaatcctt ccgtggacaa tgccaccgat cataggaggc
     2581 ttccttgcga caggagctag ttggcgagga gctctattac aagttgtttt gattttggtt
     2641 tctgtagcaa tttattatcc attcttcaaa attgcagata aacgcaatct tgaaaaagaa
     2701 aaagctactg ttggagggaa ataagatggt tatcagagta tttgatcaac agaaaaatac
     2761 ttattctagc tttgccttag aggaattaag ttactatatg aatcgggtct ttaagactaa
     2821 catagagctt gtcgaggaga aggaagcgga tatttttgta ggattagtca ataaagagga
     2881 tagaaaagac catgttctta tctcattaga caagggtaag gggagaattg agtctaatac
     2941 aattgtaggt ttacttattg gaatttaccg aatgtttcat gaatttgggg ttgtgtatac
     3001 tagaccaggg cacagacatg actttgttcc agagttacga tttgaagatt ttttagataa
     3061 acagctatct atagatgaaa cagccagtta ctatcatagg ggagtatgta tagagggagc
     3121 ggattcattt gaaaatatac tagatttcat tgattggcta cctaagattg ggatgaacag
     3181 ttttttcatc cagtttgaaa atccttactc ttttttgaaa cgttggtatg aacatgaatt
     3241 taatccatat ctaaataaag aacaattttc aaatgaatta gtacaagaat tgagtgatag
     3301 gttggataaa gaattgcaaa aaagaggtct tattcatcat cgtgttggtc atggatggac
     3361 aggtgaagtt ttaggttact cttcaaaatt tggctgggaa tcaggtctta gtatttcaga
     3421 ggagaagaaa ccctatgtcg ctgaaataaa cgggaaacga gaattgttta atacggctcc
     3481 gattttaacc agcctggatt tttcaaatcc agatgtagct gataagatgg tagaaattat
     3541 caaggattat gccaagaaaa gacctgatgt taactactta catatatggt tgtcggatgc
     3601 tcgtaataat atttgtgaat gcgaaaactg tagacaagaa ttggtttctg atcagtatat
     3661 tcgtattctc aatcaattgg atagggcttt aacgagtgag ggattagata caaagatttg
     3721 ttttctgctt taccatgagt tgttatgggt gcctcagaaa gaaaaattag ataatcctga
     3781 acgctttacc atgatgtttg caccgattac aagaacattt gaaatgagtt atgcagatgt
     3841 agattttgac aattccatac ctacgcctaa accttatctg cgtaataaaa tcatacttcc
     3901 gaattctctt gaggaaaatt tatcttatct ttttgagtgg caaaaaacat ttaaaggaga
     3961 tagtttcgta tatgactatc ctttagggcg tgctcattat ggcgatttag gctatatgaa
     4021 aattagtcaa actatttaca gagatgtatc ttatctttca aacctacatt tgaatgggta
     4081 catttcgtgt caagaattac gtgccggatt ccctcataat tttcctaatt atgtcatggg
     4141 gcaaatgctc tggaagaaga caagaagtta tgaagaattg attgaagaat acttttttgc
     4201 tttgtatggg gaaaattggc agtctgttat tgaatattta gaaaaattat ccatttattc
     4261 ctcttgtgac tattttaatg caattggcag tcgtcaaaat gatgttttag cgaatcatta
     4321 ttatatagct tacaatctag ctgataattt tttaccaatt attgaggaaa atatttctaa
     4381 gttattaaat agtcaaaagg atgaatggaa acagctcagt tatcatcgtg aatatgttgt
     4441 taagatggcg aaggctttat atcttcaagc aactggaaaa acaaggcaag ctcaagatga
     4501 atggagaaat gtgttgaatt atatccgtgg gcacgaattg ctatttcaat ctaatttgga
     4561 tgtttatcgt gtaattgaag tagcaaaaaa ttacgctggt ttccacttat aaatcataag
     4621 tatagaaaat gaaccaaggt attcagagaa gattgatcct aaatattatg aaatttaagg
     4681 atttttaaga tatttagggt cgactttcta tttatatcgt agcgaagtca ttttaataat
     4741 gatgtgtaaa agatggatca agattgagga ggaagaaaga tgaaatcaaa agaagaaata
     4801 aatatgcttg gttttacaat tgtcgcttat gcaggagatg caaggtcaga tttgatggat
     4861 gctttggcgt ttgcgagaga tggatatttt gaacaggcaa gagaattggt tgagtctgca
     4921 aacgactcaa tagtgtctgc ccatcgagaa cagactaatt tattagcgga ggaggcatct
     4981 ggagataatt ttgaagtgag ttttatcatg attcatggtc aagatacctt gatgacaacg
     5041 atgctattgt atgatcaggt caagtttttt attgatgaat atgaacgaat tcgaaagatt
     5101 gaagaacata ttggtttgca atgaggatta gtcatggaaa atttacaggt taaagcctta
     5161 ccgaaggagt ttttattagg agctgctacc gctgcttatc aagtagaggg tgcaactagg
     5221 gtagatggca aaggaacaaa tatgtgggat gtttatttgc aagaaaacag tccgttctta
     5281 ccagatccag ctagtgattt ttattatcgt tacgaagagg atatagcttt ggcggcagaa
     5341 catgatttgc aggctttgcg tttatctatt tcttgggttc gtatatttcc tgatatagat
     5401 ggggagccta atatattagc tgttcgttat taccatagag tctttcagtc ttgcttaaaa
     5461 cataatgtga ttccgtttgt ttctttacat cattttgatt cgcctcagaa aatgttagaa
     5521 acaggggatt ggttgaatag agagaatatt gatcgtttca tacgatatgc tcgcttttgt
     5581 ttccaagaat ttacagaagt caagcattgg tttacaatca atgaactgat gtctcttgct
     5641 gcaggtcaat atataggagg tcagtttcct ccaaatcatc attttcaatt atctgaagca
     5701 attcaagcga atcataatat gttgttggcg catgctcttg cagtcctcga atttcatcaa
     5761 ttaggaattg agggagagat aggttgtatt catgctttaa agccaggcta tcctattgat
     5821 gggcaaaaag aaaatatttt agcagctaaa cggtatgatg tttataataa taaatttcta
     5881 ttagatggaa cttttttggg ctactacagt gatgacacgc tttttcactt gaatcaaata
     5941 ttggaagcta ataattctag ctttattatt gaagatggtg atttagaaat tatgaaaaga
     6001 gctgcacctc ttaatacgat gtttgggatg aattattatc gttcagaatt tattcgtgaa
     6061 tacaaaggtg aaaatagaca agaatttaat tcaacaggaa taaaaggaca gtcttctttt
     6121 aaattaaatg ctctaggtga atttgtaaaa aagcctggta ttccgacaac agattgggat
     6181 tggaatattt atcctcaagg gttatttgat atgttgcttc gtatcaaaga agaatatcct
     6241 caacatccgg tcatttattt aactgaaaat ggtacagccc ttaaagaggt taagccagag
     6301 ggcgagaatg atattattga tgacag
//
LOCUS       DAVLVZ010000085         6180 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000085 DAVLVZ010000000
VERSION     DAVLVZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.085"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(112..387)
                     /locus_tag="VYN77_001841"
     CDS             complement(112..387)
                     /locus_tag="VYN77_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1559259.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(494..1333)
                     /locus_tag="VYN77_001842"
     CDS             complement(494..1333)
                     /locus_tag="VYN77_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1559260.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRAVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1475..2323
                     /locus_tag="VYN77_001843"
     CDS             1475..2323
                     /locus_tag="VYN77_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1559261.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2416..3333)
                     /locus_tag="VYN77_001844"
     CDS             complement(2416..3333)
                     /locus_tag="VYN77_001844"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1559262.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3771..3976
                     /locus_tag="VYN77_001845"
                     /pseudo
     CDS             3771..3976
                     /locus_tag="VYN77_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1980 domain-containing protein"
     gene            complement(4287..4580)
                     /gene="rpmA"
                     /locus_tag="VYN77_001846"
     CDS             complement(4287..4580)
                     /gene="rpmA"
                     /locus_tag="VYN77_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1559263.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4597..4941)
                     /locus_tag="VYN77_001847"
     CDS             complement(4597..4941)
                     /locus_tag="VYN77_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1559264.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     LNSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4957..5271)
                     /gene="rplU"
                     /locus_tag="VYN77_001848"
     CDS             complement(4957..5271)
                     /gene="rplU"
                     /locus_tag="VYN77_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1559265.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5308..5395)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5490..6035)
                     /locus_tag="VYN77_001849"
     CDS             complement(5490..6035)
                     /locus_tag="VYN77_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1559266.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLIAFIMGVFLLTIYKEKLRIVKKSILSLIPLAILSIIIGFVPQTVDNIYLVPP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGVPIMMSIFYLSMLFA"
BASE COUNT         1896 a         1265 c         1019 g         2000 t
ORIGIN      
        1 aatattttcg gaacattcaa ctttttaact ctatttatta ctaaatttca taattaaaaa
       61 gcctactgac caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac
      121 agcgtcttta agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat
      181 ttctttacca gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt
      241 accaaaaccg atcaactgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa
      301 tacagcttca actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt
      361 agcgatcaaa tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac
      421 aaatataatc atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat
      481 tttatttatt tttttaggag tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt
      541 gagtttgaat aatggaaccc gtttccaaaa cagaaattgg cttttcaaca taagcttgta
      601 agctttcttt catctctttt gcccaatcat cactaccaga atatgaaatt ccaatctctg
      661 ctacagcacg ttcagaaagc gatgttatca actcatctaa ccatttttta aatgttttag
      721 ttccacgacc tttaaccatt ggctgcaatt catggtcttt catttgcatg acagcacgga
      781 tattgagaag agagctcaac aagccagtta cacggctaat tcgtccacct ttgacaagat
      841 tttccaaagt cgaaacacca atataaagct ctgtatggtt tttaacctct tctacatgag
      901 ataaaattgc ctccatatct ttaccttctt gagctaactt cgcagcctca acaacttgga
      961 atttcagggc ttggtcagtg aaggaactat caacaacagt cacatctgca gtagataggc
     1021 tagcaccttg gcgtgctgct tctaccgtac ccgaaagagc atgggacata tgaatagcaa
     1081 gaatctggcc accatctttg cataggtctt caaaaatctc agcaaagaca cctacaggtg
     1141 gctgacttgt tttcggaaga ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt
     1201 ctttcaaatc cgcatcagaa taaacaacat tatcaatcat tacagataat ggaacaattg
     1261 taatatctaa ttgctttact agttcaggtt caatagtaac agatgaatcg gttacaatct
     1321 taatttttgt catagtatca atctttctat tttaggattc agattggttt ccttacttct
     1381 aattatatca aaaaaaagat taaaaatcct aatggagtca atcaaatttt ccgtaaaatt
     1441 tgatataatc aacttataag aaaagaggtg tcctatgatt aaaaaaattt accccatttt
     1501 taccatttta ctaggtgctg ctatttatgc ttttggactg acttattttg tagttcccca
     1561 tcatctcttt gaaggagggg cgacaggcat taccctcatc accttttatc tttttaaaat
     1621 ccctgtttcc ctcatgaacc tgctgattaa tattcccctt ttcatcctag cttggaagat
     1681 ttttggagcc aaatccctct attctagttt actaggaacc ttagctttgt ccggctggtt
     1741 agcttttttt gagcatattc cccttcatat tgatcttcaa ggtgatttac taatcacagc
     1801 ccttatagcg ggaatcctat tgggaattgg ccttggaatt atttttaatg ctggaggtac
     1861 aactggcgga actgatattc tagctcgtat tctcaacaaa tacactcata tatccatagg
     1921 aaaactgctc tttatcttag atttttgtat tctcatgttg attctcctaa tcttcaagga
     1981 tttgagattg gtttcctaca cgcttttgtt tgattttatt gtttctcgtg ttattgattt
     2041 gattggtgaa ggaggatatg ccggcaaagg ctttatgatt atcacaaaac gtcctgacca
     2101 acttgctaag gcgattaatg atgacctcgg aagaggtgtt acttttattt ctggtcaagg
     2161 ctactatagt aaagaaaatt tgaaaatcat ctactgtatt gtcggaagaa atgaaattgt
     2221 gaaaacgaag gaaatgattc atcgaatcga tcctcaagcc tttataacta ttacagaagc
     2281 ccatgaaatc ctaggagaag gcttcacctt tgaaaaagaa taaaaagagg taatgtcgtg
     2341 acctcaaaag ttagactaaa tcatctatct tttgggttac agacaacctc ttttttgttt
     2401 tatttactca agctcttaag accaattccg agttacttct tcatcagcct ttaactgatc
     2461 cactaattgg tcaactgagt caaatttggt catatctcga atgcgatcaa gccaataaac
     2521 catgacggtt tccccataaa tatcttgatt aaaatcaaaa atattgactt caaaacgtgc
     2581 ttcttctcca tcaaaggtca catttttccc gacactagcc atagcacgat acttctgtct
     2641 ttgaatctca acatcaacga cataaacgcc atctgctggc atataagtac ggtctaaaag
     2701 cactaaattc gctgtcggat aaccaattgt acgaccacga gcattaccat gaactaccat
     2761 acctcttgat ggaagcggtg cccccaaaag ttttcctgct tctttcacat ttccatctaa
     2821 aatagcttga cggatacgag ttgaactaat ctttcctttc tcatcttcta caggtggaac
     2881 aatgataact tctccatcaa agtaatcctt taaatcttct gctgtttttt tgtcagaacc
     2941 aaatgtataa tcaaaacctg caacaataat tttggcattc atagccttga tataagttgc
     3001 aaagaattct tgtgcagtga gactagcgaa ttgactacta aaatcaagga gatataattc
     3061 ttctacacct tcgcgcttta attttctttc acgttcagca gggttcaaaa tatgcaaaaa
     3121 caaatctgga tgataaggct ctaaagcgat ctttggagat tcattaaagg tcataacgac
     3181 gataggcaat aaatcctttc tcgcagcctt gttggcaaca cgaaataatt cttgatgccc
     3241 cttatgtatg ccatcaaaat agccgagaac aacgactgaa tcagatggtg tgccaatatc
     3301 tttttggttt tttataggaa tagtaataat cataaaataa ttatatcata gcgatagcta
     3361 tttctggaac agaaaatctg aaatgttgtt tttttcacat gaagtgtacc tattttcaaa
     3421 aagcacttta ttctatcgtt gcttaactat gaactttgca atattcttct caaaaacttg
     3481 taggacatct tcaaaatttt gcaaggagtg attagacttg ttcggtaacc ataaagtgtc
     3541 atactatgct tatgtatgaa aaagcaatgc aactaactcc tgagaacttt aaattactaa
     3601 ttggtgccga aaaggtagaa tttagaatca aggtacacct atggctgtaa aatttacaaa
     3661 atgagacaac ttgagcaaga tgtttgaaga atttcctaaa ctccctgatt tgaagcaagt
     3721 cactttccct gatgacaaag aaaaaagcca aaacagcaaa gaaaaactag atgactgctt
     3781 tccaacaact cccatctagt gtgcttcaga ctgggctatt tttctctcca tctgttaact
     3841 tggattctca gaccgtttca gctaaagaat atcttttccc ttatcagaag gaacggctca
     3901 agccattcag acaagtgaag ggacgacaag ccaatatttg aaaccagata ccagttctta
     3961 tagtcaattg aaataaaatc tgaagaaatc gagtaggaaa ctcatatcaa tttttaacag
     4021 tgttctattc cagattcata ctcaatgaaa attaaagtgc aaactaggaa gttagccgca
     4081 ggctgtactt gagtacggca aggtgaaatt gacgtggttt gaatttgatt ttcgaagagt
     4141 atcaatccac tatacatatt aaaaagtatg ggtatcaaaa taaaaaaaca ggctctccga
     4201 aaactcggaa agccttattt taagctgttt ctagcttcct cacctttaca tttcaaggtt
     4261 cgggataaaa ggttcaatga acctttttat tttgcgattg ggtaaacaga aacttgtttt
     4321 ttatcgcgtc ctttacgttc aaagcgtact acgccttcaa ctttagcgaa caaagtatcg
     4381 tctccaccac gaccaacgtt tacacctgga tagatgtgtg taccacgttg acggtaaagg
     4441 attgatccac ctgttacagt ttgtccgtca gctgctttag ctccaagacg ttttgcttgt
     4501 gaatcacgtc cgtttgatgt agaacctcca ccttttttgt gggcgaaaag ttgcaagttg
     4561 ttaagagtca tttttaacat aatgttttcc tccgtgttag ttttctgtga taactctggt
     4621 ttggacgaac tcagaagagt tctccgataa gtttgccata cctaagaaaa atgattcaaa
     4681 gaataactgg gtcatttctc tctggtgtga aggaagatct tttggtattt caaccatcag
     4741 atagccacct tcatcttcgt ttaattctag gattggttca tagcctgcaa atttctcaat
     4801 agaattgaga aagttaatgg caagcgtaga aaccgatgca cacacgacat ctaagccgta
     4861 ttcgccactc tcggcgtgtc cagtaatttc cgcactcctc agctcgccat cttcggctct
     4921 ctcaaagact gcttgtatca tgtgttctcc ttaaaattaa gcgttaattg cgttgatgac
     4981 aacttttgta tatggttgac ggtgaccttg tttacggtgg ctaccttttt taggtttgta
     5041 cttgtaagta acaactttct tttgttttcc ttgtttttca acagttccaa ctacagtagc
     5101 tccagcaaca agtggagttc cgacaacagt gttttcacca ccaacaagaa caacttcgtt
     5161 aaaagtaact tcttgaccag cttcaacgtt caatttttca atgtaaactg cttgaccaac
     5221 ttcaacttta acttgttttc cgccagtttt gataattgcg tatgtgctca ttatgcacct
     5281 cctatgattt ttatgggttt ccccgatttt tttgtgaaga ctcgcctagc atcgtgggac
     5341 aaaccactta aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca
     5401 tagtcaactc ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat
     5461 caagcttttt cttttacttt tttctgccac taggcaaaaa gcatactgag ataaaaaatg
     5521 ctcatcataa taggaacgcc aaggacaaaa ctactaagca ggctaacaaa tatgaaccct
     5581 tcacgcaaaa aaggagtgtg cttagttcgg aaataatctc caaaagctag cattgttcgt
     5641 ttgatattcc ctgtcataaa ggcattattg taggcaatac ccgaaacctc accaaaagca
     5701 gttgtcacca gtcccataca gaaggccaag ggcggcacta gatagatatt atctacagtt
     5761 tgtggcacaa aaccaataat tattgatagg attgctaaag gaatcaagga cagaatagat
     5821 tttttcacaa ttctcaattt ttccttataa atcgttaata aaaagactcc cattataaac
     5881 gctattaagg ttaaaagctt atccctaaca tccgaaacat tattttgtat gagctctacc
     5941 gaaagaaaga caacatttcc agcttgtcca gctacaaggg tattccctcg aacaataaaa
     6001 gtgtaagcat ccacatatcc agcacaaaac gtcaaaaaag tgctaacctt ttagactgac
     6061 gtggtatttt tcttataggt aataacctca ttttccctcc cattgtattt tctcttagaa
     6121 atattgtacc atttctttct aaaaaatcgt aggctaccat ttagatttta ctattagcat
//
LOCUS       DAVLVZ010000086         6176 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000086 DAVLVZ010000000
VERSION     DAVLVZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.086"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            150..458
                     /locus_tag="VYN77_001850"
                     /pseudo
     CDS             150..458
                     /locus_tag="VYN77_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            637..1296
                     /locus_tag="VYN77_001851"
     CDS             637..1296
                     /locus_tag="VYN77_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1559267.1"
                     /translation="MKNLTKIKFKENGEFNHFSGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1321..2388
                     /locus_tag="VYN77_001852"
     CDS             1321..2388
                     /locus_tag="VYN77_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1559268.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            2491..3501
                     /locus_tag="VYN77_001853"
     CDS             2491..3501
                     /locus_tag="VYN77_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559269.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALTRALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            3513..5204
                     /locus_tag="VYN77_001854"
     CDS             3513..5204
                     /locus_tag="VYN77_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1559270.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5207..5917
                     /locus_tag="VYN77_001855"
     CDS             5207..5917
                     /locus_tag="VYN77_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1559271.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2075 a          851 c         1175 g         2075 t
ORIGIN      
        1 tcgcttacca atatttgcta aactttcaga aaaaaagttg gagtatatga tgctaggtga
       61 ctgcgtcatg ttagtaaacg aaatggaaat aactgatcat agagtagata acttatttga
      121 aaaagggaaa aatgagataa aagattcaag tagtagattt agcaatagga catataaaag
      181 ctttagaaaa agtatctgaa aaaacagatg tttatatcta taacctaggc tcgggagaag
      241 gcactagtgt attacaactt gtaaatacat ttgaaagtgt taataagatc cctattcctt
      301 ataaaatagt tccaagacgt tcaggagacg ttgcaactta ttatgcaaat gcagacaaag
      361 catataagga attaaattgg aggacaacaa aatcgattga agacatgtgt agagatacat
      421 ggaattggca agcaaaaaat cccaatgact ataattgatg atactgtcta tatgagtgct
      481 gtattttgga aaaattactt aaccttttaa tgaaatgcat atttaatgaa agataaataa
      541 aaaaaagttt gacagcgctt gacaaaggaa acggtttcat tgtataatgt aaacatagtg
      601 acaccgatgt caaatataaa aaaagaaggt ctttatatga aaaacttaac aaaaattaag
      661 ttcaaagaga atggggaatt taatcatttc tctgggaata cagttgtagc aaatctttat
      721 actaaacaag atttgatgga agttgttgat attattcaat cacgttatag agaattacca
      781 tttatcgata agtttacttt aactccaagg aattctattc acatgactgt aattgaattg
      841 ttgtgccatg aaaatcgtga aacggagttt tggagcagta atcttcctct agatacacct
      901 ttacaggaaa tacatgatta ctttgctaaa cagcttgaaa tttttccatt gttagatgaa
      961 gaaattcata tgcgtgtaac tgaaatggga aaacaaaaca tactagttga acctgcagat
     1021 gaagcttctg caaagagatt agaagaaatc cgtacttatg tctcagaaaa agcaggtgtt
     1081 tgtttcccta atcatgatag atatcaattc catatttcaa ttgggtatct tcggattcct
     1141 ctaaccgaag aggaggaaga agaatttact aaagtcagag cagaattaac tgaaatttta
     1201 ttagagaaga ttccaactat tactgtaaac cgtattgact atactgtatt tgaagatatg
     1261 agacaatttg ttccatatca cgaaaaattt aaataaaata ataaaaaagg agaacttatc
     1321 atgaaaaaaa tgaaagtttg gtctactgta cttgcaacgg gagttgctct tactacactt
     1381 gctgcttgct ctggaggttc aaattctacg actgcttctt catctgaaga aaaagctgat
     1441 aaaagtcaag aattagtaat ctattcgaac tcagtctcaa atggtcgtgg tgattggtta
     1501 actgctaaag caaaagaagc tggttttaat ataaaaatgg ttgatattgc tggcgctcaa
     1561 ttagcagacc gtgttattgc tgagaagaat aatgcagttg cagatatggt atttggaatt
     1621 ggtgctgttg actcaaataa aattagagat caaaaattac tagtacagta caagcctaaa
     1681 tggttagata aaattgatca atctttatca gataaagata attattataa tcctgtgatt
     1741 gttcaaccat tagttttaat tggggcgcct gatgtaaaag aaatgcctaa agattggact
     1801 gaattaggta gtaagtataa aggtaaatat tcaatttatg gtcttcaagg tggtacagga
     1861 cgggcaattc tagcaagtat cttagttcgt taccttgatg aaaaaggaga tttaggcgta
     1921 tcagaaaaag gatgggaagt tgcaaaagaa tactttgcaa atgcctatac tcttcaaaaa
     1981 ggtgaaagct cagttgttaa agcgttagat aaagaaagcc caattcaata cggtatgatc
     2041 tggggttctg gagcgttaat cggacaaaaa gaacaaaatg ttgaattcaa agttatgact
     2101 cctgagattg gtgtaccatt tgtaactgaa caaactatgg ttttaagcac tagtaaaaaa
     2161 caagcgttag ctaaagaatt tattgattgg tttggtcaat cagaaattca agtagaatat
     2221 agtaaaaaat ttggatctat tcctgcaaat aaagatgccc tcaaagagct accagaagat
     2281 actaagaaat ttgttgatca agtgaaacca caaaatattg actgggaagc tgttggaaag
     2341 catttggatg aatgggtaga aaaagctgaa ttagaatacg tacaataaga aataagagtt
     2401 attatttatg tccaagtctc tctattattt ctaaatttat ggaagaaata aagagaggct
     2461 tttggtttag taatagaggg agttagtgag atgattaaat ttgataatat tcaaattaaa
     2521 tatggtgatt ttgttgcaat tgataatctg aatttagata tacatgaagg ggaattcttt
     2581 acatttcttg ggccttcagg atgtggtaaa tcaactactt tgagagcatt ggtaggtttt
     2641 ttagatccat catcaggaag tattgaagtt aatggaacag atgtcactca tttggaacct
     2701 gaaaagcgtg gaattggtat tgtatttcaa tcttatgcgc tatttccaac tatgactgtt
     2761 tttgataata ttgcatttgg tttaaaagtt aagaaggtag ctccagatgt tattaaagct
     2821 aaagtatcag cagtggcagc aaaaattaag atctctgatc aacagttaca gcgtaatgta
     2881 tcagaattat ctgggggtca acaacaaagg gtagcattga ctcgtgctct ggttcttgaa
     2941 cctaaaattc tttgtctaga tgaaccattg tcaaaccttg acgcaaaatt acgtgtagat
     3001 ttgagaaaag agttgaaaag acttcaaaaa gagttaggta ttactacttt atatgttact
     3061 catgatcaag aggaagcctt gactttatct gatagaattg cagtctttaa caatggatac
     3121 atcgaacagg tcggtacacc agtagagatt tatcataatt ctcaaactga atttgtatgt
     3181 gattttattg gagatattaa tgttttgacc gatgaaacag tccacgaagt attactgaaa
     3241 aatacaagcg ttttcttaga agataaaaaa ggatacattc gattagagaa agttcgattc
     3301 aatcgtgaaa ctgaacaaga tttcattcta aaagggacaa ttattgatgt tgagttttct
     3361 ggagttacaa ttcactatac aataaaagtt tctgaaagtc agattcttaa tgtaacaagt
     3421 attgatagtc aggctgctat tagatctgtc ggagaaagtg tggaattatt tatcacacca
     3481 tcagacgttc tgcaatttta aggaggtgca gtatgcgtca taaattaaat ttaaaagatt
     3541 ggcttattcg tttagggtta atctggttct tagtaacatt tattatttat ccaaactttg
     3601 atctagtagt gaatgtattt gtaaaaggag gagaattttc ccttgatgct gtacatcgtg
     3661 ttctaaaatc tcagagggca cttcagagta ttatgaacag ttttaagtta gcattttcac
     3721 ttattattac agttaatgtc gtaggtattc tttgtgttct atttacagag tactttgata
     3781 ttaaaggtgc taaaatttta aaattaggtt atatgacctc tttaatttat ggaggagtgg
     3841 ttttagcgac tggatataaa tttgtctatg gtccttatgg attgattaca aaatttttac
     3901 aaaatgttat cccttcttta gactctaact ggtttattgg gtatggtgca gtcttattca
     3961 ttatgacatt ttcaggaact gctaatcata cattgttttt aacaaataca attcgaagcg
     4021 ttgactatca cactattgag gctgctcgaa atatgggagc aaaaccattt actgttttcc
     4081 gaaaagtagt gttaccaacc ttaattccaa ctctatttgc acttactatt atggtttttc
     4141 ttagtggttt atctgcagta gcagcaccca tgattgttgg tggtaaagaa tttcaaacta
     4201 taaatccaat gattattaca tttgcaggga tggggaattc tcgtgattta gctgccttac
     4261 ttgcaattat tttaggtatt gcaactacaa ttttgcttac tatcatgaat aagatagaaa
     4321 aaggtggaaa ttatatttct atctctaaga ctaaagcgcc tcttaaaaaa caaaaaattg
     4381 cgtctaagcc ttggaatatc attgctcaca ttgtagcata tggattgttc acagttttca
     4441 tgcttccact aatttttata gtattatact catttacaga tccagttgca attcaaacag
     4501 gtaacttaac attatcaaac tttactttag aaaattatcg cttattcttt agtaatagtg
     4561 cggcattctc tccattcttg gtcagcttta tttattctat tattgctgcg acaacagcaa
     4621 caattctcgc agttgtattt gctcgtgttg tcagaaaaca taaatctcgt tttgatttct
     4681 tatttgaata tggtgctcta cttccttggt tactaccaag tacactttta gcagtaagtt
     4741 tattatttac ttttaatcag ccacaatttc ttgtcttgaa tcagattttg gtaggtagtt
     4801 tggtaattct acttattgca tatatagttg taaaaatccc attttcttat agaatggtac
     4861 gtgctatttt atttagtgtt gatgatgaga tggaagatgc agcaagaagt atgggtgctt
     4921 caccttttta tactatgatg aaggttatca ttccatttat tttaccggtt gttctctctg
     4981 ttattgcttt aaactttaac tctttattaa ctgacttcga cttatctgta ttcctttacc
     5041 atcccctagc tcaaccatta ggtattacga ttcgatctgc aggtgatgaa acagcaacat
     5101 ctaatgcaca agctctggta tttgtttata caattgttct gatgattatt tctggaacgg
     5161 tattatactt cacacaaaga ccggggcgta aagtaaggaa ataatcatga cagccactag
     5221 tcttgggtta tcaaatattg aaatagttgt caggattgtt ttatcagtag tcattggtag
     5281 tataattggt ttagagagag ggagcaaatc ccagcctgca ggcatccgaa cttatagtat
     5341 tgtttgtcta gctgcatgtt tgattatgat gacgaatgaa tacgtatctt ataaatttgg
     5401 gacaggagat cctacacgat taggagctca agttatatca ggtgtgggtt ttctaggcgc
     5461 tggaacgatt cttattacag ataaaaagaa aattacaggt ctgacaactg cagcaggcat
     5521 ttgggcttcg gcaggaattg gattagctat tggagtaggt ttttatgagg gagctctttt
     5581 agtagccatt tctgtttggg gtgtgatatc catgttccaa ccactaaaaa aatatctgca
     5641 aaatcgttct aaaatgattg aattgtatat agtagttaaa tctacagaag cctataatcg
     5701 tgtattggtt tattgtgcag aaaatggtat cagaatgacc gattcaagaa cagcgtttgg
     5761 agatgttaat tctgacagaa ttgaatactt tgatgttccg gacaaaaaaa tagcgtcatt
     5821 tattactctg gaattatcag gtagatttga acatcttcga ctgatggaag aaattgctaa
     5881 tattgtaggt gttatttatg tcgaagaaat tagctgaaat ttagtaaaca aagggacaga
     5941 tgtttttgtc cttttgtttt ttagatgttt taatcatcta atttttgagt agatttttgc
     6001 tcagaggttt ttatgaaaga ttataaaatc aattttgatt taggtaaaat agaatatttg
     6061 ataataattg tttgatacaa gtatacaaat ttatttcttt ttatgatatt tgtgaaatgg
     6121 tgtttgcctt tcatttacct cctgatgagt tgataacaaa tgttatcttt aaagaa
//
LOCUS       DAVLVZ010000087         6053 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000087 DAVLVZ010000000
VERSION     DAVLVZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.087"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..831)
                     /locus_tag="VYN77_001856"
     CDS             complement(<1..831)
                     /locus_tag="VYN77_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1559272.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDEAVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLFLKLNHNTLVDIKSKLESLKLKHSRYYISK
                     I"
     gene            complement(833..1390)
                     /locus_tag="VYN77_001857"
                     /pseudo
     CDS             complement(833..1390)
                     /locus_tag="VYN77_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1377..1826)
                     /locus_tag="VYN77_001858"
     CDS             complement(1377..1826)
                     /locus_tag="VYN77_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV1559273.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVVPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(2238..2633)
                     /locus_tag="VYN77_001859"
     CDS             complement(2238..2633)
                     /locus_tag="VYN77_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1559274.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFN"
     gene            complement(2729..2983)
                     /locus_tag="VYN77_001860"
     CDS             complement(2729..2983)
                     /locus_tag="VYN77_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1559275.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(2993..4327)
                     /locus_tag="VYN77_001861"
     CDS             complement(2993..4327)
                     /locus_tag="VYN77_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1559276.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVIELCDVHANLFKNAIKDVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(4327..4560)
                     /locus_tag="VYN77_001862"
     CDS             complement(4327..4560)
                     /locus_tag="VYN77_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1559277.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFNPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            complement(4977..5159)
                     /locus_tag="VYN77_001863"
     CDS             complement(4977..5159)
                     /locus_tag="VYN77_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559278.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDNAVVGLGKLFIAD
                     KLMDTDRWLIKPEDKK"
     gene            complement(5161..5940)
                     /locus_tag="VYN77_001864"
     CDS             complement(5161..5940)
                     /locus_tag="VYN77_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559279.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRVGGITIAK
                     QVMKTVPNKALTKTLWYLILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT         1788 a         1363 c         1031 g         1871 t
ORIGIN      
        1 tatctttgaa atataatacc tagagtgttt caattttaat gattccaact ttgatttaat
       61 atctactaat gtattatggt ttaactttaa aaacaactta ttataattac catagagctc
      121 tgtaagagca ttaaagtatt ctttcactag agctatagcg tcaatcttag atgtttcttg
      181 gtttagtttt atcaactccc ctttcaattt tttccattta aagttggaat ttaaaagata
      241 atctaaatca atatagaaat ctattataac agcctcatca tctgggctaa cagcttttac
      301 ctcatttatt ggtaagtcag tatgttgcga ataatttctc aaagaatggc aaaatcgata
      361 agcaaaattg gtatcatact gatagcttgc aaaaccatgt atacaatcaa actcctcact
      421 atctttggaa tatttttgtt taatttggtt ttcgttaaaa tcgacaaaca attttccact
      481 ggataataaa tgaatcatga aacgatttac atctgcctct tcaatttcta attcctttga
      541 ctcaagttta tttttaatac ttaaaaaatc cgagacatct gaaataaaca attcgaacat
      601 attcttgatt ttttgaaaat tcttttgaat ctcaaaaatt tggtccgaga ctgacattat
      661 ttccttggtt ggttcaatat cagagatgac aatacgtttt tcattgttgt taatttctaa
      721 ataagctaat cgctcgataa tatcactatc atttaattca gcatactcaa catcaatttg
      781 gcgtaactgc ttctcagata gagagcttac ttcctctttt acatcgctca tcttattcct
      841 cccactcgct cttcagcaag ccatatatca gactgtcaca gcggtttcct tgaacatcct
      901 ttcggtctct tatgcgagct tcgagggtaa agccaagctt ttccgcgact cgtttacttt
      961 gaaggttata tccaaagcaa gttagttcaa tcttgtgaag acccaaatct ttaaaggcta
     1021 agtcaatcaa ggcacgcgca gcttctggca cataacctca cccccaatag tctgggtgta
     1081 aggtatagcc gatttccagc acatcatctt catggcgatg gttgaaatcg acagagccaa
     1141 cgatcttatc cgttcctttg acgacaatcc cgtagcctgc tgggagattt tccttttgat
     1201 tacgctccgg aagaatgtgc tccagataat aaatctcatc ttccaaggtc ttgactggag
     1261 gaaaacctgc tggataggcg acctctggca aactagcata gtcaaagata tcctcagcat
     1321 ccgccacagt acggactcgt aaaacgagac gttctgtttc gattttatct ggcagctcag
     1381 ttcttgccat ccttcttcct cgcttttttg atgaaactgc ccttcatatc tacacgcttg
     1441 tccagatagc gataaacgcg ctgatatcca tctcccatga aataggttgg ggcaaacagt
     1501 tgatttttaa aatgtccctt ttcatccagg agttctggaa caacaagtcg ctcaagaatc
     1561 ttggcaaaga tgtggcaaat accgtcttcc tcaacaatcc tatctacccg acaatctaaa
     1621 acaagtggac aggcgtctaa aataggagtc tgagttcgtt cagaaatttc ataatgcact
     1681 cccaaacgtt ccaatttctc ctgatgactg ataaaaccag cctgctccat cgcaagcata
     1741 gaagtttcat cagaaatatt cacagtaaat ttttgatact gtttgatctg ctctgcggca
     1801 ttctctctcg caacgactcc aatcacaacc caatctccta gactataaga ggaactacag
     1861 gtcgtgatgt tatagccaaa attctaatct tgatatccta aaataaaaac aggaaaacca
     1921 taatatagtt tacttgtgtt aaaagattgc ttcataacaa ccccctttga ctaagacgta
     1981 aaagaaaagc cctgccatct acatgacagg gacgaatgtg tttatccgcg gtaccaccca
     2041 atttcgggcc aagcccgcat ctttagtctt taaaataaaa agacagaaat atttcatttt
     2101 ttcagtcatt agcaaccagt tttgacacat ttgtggactt ctcagcgccg ccactttctg
     2161 taaaatgtgg gattcaaaac acctctaatg gctctattgt agcaagattt tttcggaaat
     2221 gcaaggctca agaaaaatta attgaacttg atgccatttt ctttcaattt cttgatggta
     2281 gctgggccga ttcctttcaa gtcaaggagt tctttttcag tccagttttt gaaatctgaa
     2341 gcagacttga ttccttcatc atagaaagtt ttggcacgat caagtggaag tccacccaag
     2401 tttgctgcaa aatcttctac agaattagct actttttgag cttgctcttt ggtagcttct
     2461 actgcttgtt caactttttt agaaacagcc ttaccagctg cgcttgctga ctgttctgca
     2521 cgcttcacga ctttttttgt ctcatctaca accttagtca cagtacttga aaaggcacct
     2581 gcgcgacgga ggccatttcg taattgtttt ttacgattga gtttctttga catgataaaa
     2641 tcctttcaat aaaaaatccc tgctctgaca aggatttaac ataaacattc tatcaagatt
     2701 ttagtgaaaa atcaagaatc tttctcgttt aataatttcg ctcaccaaac aagccttctg
     2761 gcaaatcatg gaatcccttg cctactttga agttttcaaa cttaccaagc aagaactgat
     2821 aagcatccaa gcgactctcg tagcgaatca tggcatcttt ggcacgctcg tcctggtctt
     2881 cttcgtaaac tttttgactt tccttgtgcg ccatatcgtt aatcatgatt tgggtattga
     2941 cccaagcttc aaattccttc ataaattctt gttcgtaact cattttttct ccttattcta
     3001 atccacgaaa atagtccgta tcaatctcac ggtagggctg gatatcctga acatactcca
     3061 acactccttg gaattctccg tcttcatcgt gtactgcagc ataggtgatg tggacaaact
     3121 tacctcgcga ctcagacttg aaccacattt catacttgtc tttgcttccc tcacgaagcc
     3181 ccttcatgat agttttgacc ttgtccaagt acttaggcgg atggcagagt tcgacattgc
     3241 gcccgacttg ggacggcgtc cgtttgaaaa tcatctcatc agctggcgta ttgtcattgt
     3301 aatactggaa aatatcttct ttattgacaa aggtaatctc catagggaga tgattgagga
     3361 tgagattggc ctgctcgact gaaagatagc cattaccaaa agcctgttga ctatggcggt
     3421 ccagcacagc ttccttttcc ttaggggtaa aggtaatggt aaaatggcct tctggcgtat
     3481 ctatgacttg ttgaacttga ccttctgccg tatctagctg tacaggctcc tctgcaatct
     3541 tttcctcaat aaagatctgt cgttctggca cccatttctc tgacggacgg atgatggcat
     3601 agccataggc atcgctctcc tccgcaatct gaagccagtc atcctgagta aaagactcaa
     3661 ggagaatcat gaggaggatg gactcttcct tgaaaatcat actttcaaac tctgtcgcaa
     3721 aagcttcaaa agcttccttt acactgctaa ttgacacttc tggtagtgac ttggctgtcg
     3781 ttagagctgt ttgaaagagt tccctaatct gatcatccac tccccacata actttgggag
     3841 gtgaatcgtg tccatagcgc tccatgatag gaaagaagag ttcttcctta cgttggtaat
     3901 ggatgtcaaa ttgccccaca agccccatct gacgcaccaa gcccttgcgc atctccgcaa
     3961 gcatttcctc gtcttccatc aactcatagg tatctaacaa tctacgaatg cgaatcaagg
     4021 ccgcacggag agccagattt tcttctttga agacacgaac tgggtgacct ggatgctcag
     4081 tatctgaaac ttcgacatct ttgatagcat ttttaaaaag attggcatgg acatcacaga
     4141 gttctataac atcttcaaag gtgacacctg agtctgagtt catcagctcg tgctccataa
     4201 gggaaatctc aatggctgag acacccgcaa aggtcgcatc aaaacgctcc tgaaccgact
     4261 caggagaggc gccattatgc aattctaata aaatatcccg taggacatga atccgttcat
     4321 ctgccattag tctaatccaa tcacttcgta gccattcgct tccagtgtgc gtacaatctt
     4381 gtccatagga gttcctgcta gcttagaacc ctgtttaagt gatactttac gaccaactgt
     4441 attgcgcatt aagggattag caagggggtt aaaacccaac tccactagaa tttccaagac
     4501 ttctggatgc ttgtccacca cttctgcaac aggaattgac acatcgatga tattgtccat
     4561 gacgacctcc attgtatgtt ctaatactca atgaaaatca aagagcaaac taggaagcta
     4621 gccgcaggtt gctcaaaaca ccgttttgag gttgtggata gaactgacga agtcagtaac
     4681 atatatactg caaggtgaag ctgacgtggt ttgaagagat tttcgaagag tataaaatga
     4741 ttttactgct tatattatac catatggaaa gagaataaaa aactagcagt ggagttcctg
     4801 ctagttctct gcaacttgga ttaccatcta gataagttta aagagccaat ccaatatctt
     4861 aaacactatc ttgtaaaaca gcaattggac tgcaaaagag atgatcatcc aaaagaattt
     4921 cacaatccca ataatcggtt tgataaaaat aatggcaaga agtccccaga aaaatttcat
     4981 tttttatcct ctggcttaat gagccaccga tccgtatcca ttaacttgtc ggcaataaaa
     5041 agtttcccca gaccgacaac ggcattatca tctttcttta tcgttttcat tacttttaca
     5101 ccttgcacgg tcaaaaagta attaccataa attttagcta gagtttttat gagtcccata
     5161 ttactctcct tcgatgattt cagcctcttt tcggattgtt tcaacatctt cttgatattg
     5221 aacttcacta tagttaacta gcttggacaa ttctttctgc aagctttcac ccatcggttt
     5281 catagttgca aaggttaaac cacctgaaat gataccaccc aagataggaa taaatttccc
     5341 cattcctttg gccagccctc ccttggtaag attcacacca aatattctta agactttttt
     5401 caaaataaga taccaaagcg tctttgttaa agctttatta ggtactgttt tcattacctg
     5461 tttggcaatt gttataccac caacacgtag caaagcagca gttccattca cccccaacat
     5521 gacgcctaga taaagcaaaa gggtattttg agcatcttca ctcaactcat cgcgtgaagc
     5581 ccaaagatcc tcataaccat aaatataacc taattcttgg gccaatttca gagagaaacc
     5641 ataaaattga gccacatcag ctggaatggt aattgccata gcaagccctc caggtaatcc
     5701 tgccaaaaca gaagtcccac tcgctaataa aacattatcc ctaatacagg cattagccac
     5761 tcgatctaag atttcttgag ataagagaga cgttggacct tgttccaata aggtaggaat
     5821 gtcctgtgga cccaattctt tggaaaactt atccactaaa aatttcgaac gatcaacctt
     5881 aacaactggt agttttacca cttgttgtaa tacttgcata gctaagtctt gttcagccat
     5941 atacaaactc cttatttctt ttttacacct tatcatatta tatttcctac aaaaaaagca
     6001 ttttctaatt agataacatt aaaaaataca gaaccccttt tttatagtaa aat
//
LOCUS       DAVLVZ010000088         5203 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000088 DAVLVZ010000000
VERSION     DAVLVZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.088"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(263..814)
                     /locus_tag="VYN77_001865"
     CDS             complement(263..814)
                     /locus_tag="VYN77_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1559280.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1061..1885)
                     /gene="nadE"
                     /locus_tag="VYN77_001866"
     CDS             complement(1061..1885)
                     /gene="nadE"
                     /locus_tag="VYN77_001866"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1559281.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(1882..3342)
                     /locus_tag="VYN77_001867"
     CDS             complement(1882..3342)
                     /locus_tag="VYN77_001867"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1559282.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3458..3946)
                     /locus_tag="VYN77_001868"
     CDS             complement(3458..3946)
                     /locus_tag="VYN77_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559283.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(3936..4145)
                     /locus_tag="VYN77_001869"
     CDS             complement(3936..4145)
                     /locus_tag="VYN77_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1559284.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(4530..4982)
                     /locus_tag="VYN77_001870"
     CDS             complement(4530..4982)
                     /locus_tag="VYN77_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559285.1"
                     /translation="MIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIV
                     AIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMMMNTSNDLFI
                     KAIELLPFYSLPVFVASNTMSISQYLVLITTIILLYFFTLKKSKKYSF"
BASE COUNT         1584 a         1142 c          939 g         1538 t
ORIGIN      
        1 tattttaaat ttaacaacag ctaggattac actataaaaa gcacattacc acctgttcga
       61 ctggagaatc tagtcgatta gctccctctt agttacaagc ttaaagcgat tttttaacta
      121 atactaaacc agattaaaga aggaactaga acatctgtct tgtactaatc aatatgctat
      181 tttaaaattg caaataaact tggaatgcta attattatat ttacaataaa tagttgactt
      241 cgatagaaac agaaaataga attcaacatt cttccctact tataccataa taaataagat
      301 ccgcaagata gccttttctc tctacaccat taacaatggt ttgtagatag gacattccag
      361 ccttttccat gacacgacct gaagctgggt tgagactggc ataacgtgct ctgacctttt
      421 gaaaacctgc ttgagtaaaa caaaagtcca agacagcttt caaggcctct gtcatcatac
      481 catgtcccca gtaagccttg cctagcacat agccgatttc acagcttaaa tcagcctcgt
      541 ctatcttaac gatgctgata tctcctatca cttgctctgg gttttctttt agacaaatgg
      601 cccatttata atagttgaga ttagtatagg aagcaaccca attgcgaatc gagtttcgag
      661 tgatttcgac atcaggatgg ggatcccagg taacataggt cagattctca gcggatgaag
      721 cccaattttg aaacatggct tctgcatcac tctccacaaa tcttcttaaa atcaaacgtt
      781 ctgtctgtaa tatttgcgta ccgatagatt tcatgatgct acctcacttc cttatttcta
      841 tgctcgtagt aaaaagactc aactcagtaa cagctagaaa acgagtttgt cttctaattt
      901 cttgacgcag ttgctgcatg gtttgaaatg cgctctagca atctttttca aacctagtca
      961 tcgttgcagg ggtaagacga tgaaatcgaa ctcttcgagt taccgaatct gcctcatttt
     1021 cttatttcta aactcggggt tcaagatccc ccggaccttt ttactcccaa aagtcatcaa
     1081 atacggtgat gggtaagtgg cgtttgtgtt ggcctttgtg ccaccagttt tcaatggtcg
     1141 cttgagcttc tgggctgatt gttttgcctt ctaggtaatc gtcaatctct gcataggtga
     1201 ctccaagtgc gacttcgtca gctaggcctg gtttgtcttc ttctaggtct gccgttggga
     1261 ttttttcata aagggctggc tctgcgccaa gtttctgcaa gagttgtttt ccttggcgtt
     1321 tattgaggcg gtaaagaggg agaatatccg caccgccgtc accaaacttg gtaaagaaac
     1381 ctgtgatatt ttccgcggcg tggtctgttc caatgaccgc tccgctatgg gaaccagcaa
     1441 gggcatactg agcaatcata cggcaacgtg ccttgatatt ccccttgttg aagtctgaaa
     1501 caggactacc tgtcgcttca actgcagctg tcatggcatc agctgattcc ttgatattca
     1561 caaccaagct gacatctggc tggatgaagg ctagggcttt ttgagcatct gcttcatcag
     1621 cttgcactcc gtatggcagg cggacagcga taaatttgta gctatcgtct cccgtttcag
     1681 ctcgcagttc ttccatagct aattgcgcca aacgtcctgc caaggttgaa tcttgtcccc
     1741 cagaaatccc tagtacaaag gtttttagga agggatgttt tttcagatat ctttttaaga
     1801 aatcaataga acgacggatt tcttcctggg catcaatcac tggtttgaca cccagctctt
     1861 ggataatcgt ttcttgcaaa ctcattcttc ttctccttca ccaagggctt ccttgcgcat
     1921 cttatcaatc aagtccatct tatcttgcca tacatcacgc gccaaatcca ctggatagtg
     1981 ctgcggattg agcacacgct tatactcatc ccacaacttg tcaaattcct tacgggcata
     2041 atcctgaatg tcagtcaaac taggcaagtt gtaaactaat ataccttctt tgaagatatc
     2101 caccaagaga ggaacggcat caaaattacg aaccgtcttc ttgatgtatg tataggtcgg
     2161 atggaacatc ttgatttctg tcatgtcgct aatatccaca ccatcataag tgatgtagtc
     2221 accttctgac ttgccttttt cacgactggt aatgcgccac acctgcttct tacctggcgt
     2281 cgacactttt tccgcattat tagacagctt aatcgtattg cgcatctgac cagtttcatc
     2341 ttcgattgca acaatcttgt aaaccgcccc aagagctggc tggtcatagg ctgtaatcag
     2401 cttggtaccc acgccccaga catcaatctt ggccttttgc atcttgaggt tgaggatagt
     2461 attttcgtcc aaatcattag aagcataaat cttagcctct gtaaatccag cctcgtccag
     2521 ttgctgacgg actttcttag aaatgtaggc aatatcccca gagtcaatcc gcacacccat
     2581 aaagttaatc tgatcaccca gctcacgcgc cacctgaatg gcagctggta caccgatgcg
     2641 aagggtgtca taggtatcca caagaaagac acaatttttg tgggtcgcag cgtaagcctt
     2701 gaaagcttca tagtcattgc cataaacctg taccaaggca tgggcatggg ttcccaaaac
     2761 aggaatgtca aagagcttac ccgcacgcac gttgctggtt ccattggcgc caccaatcac
     2821 agctgcgcgt gttccccaga tggccgcatc catttcttga gcccgacgtg tcccaaactc
     2881 catcaagggt tcatcttcga taaccgaacg aatacgagct gccttcgtcg ccaccaaggt
     2941 ctggtagttg acgatgttca aaagagccgt ttcgaccaac tgacattggg ctagaggtcc
     3001 ttccacctgc acaatcggtt cattagcaaa aaccaaatcc ccttcttggg cagaacgaac
     3061 ggtcaactcc aacttgaaat tgcgaaggta atccaagaac gccccatgat aaccaagcga
     3121 ctccaaatag gctatatcac tatctgaaaa acgcaagtct tcaagatagt tcacaattct
     3181 ttccaaacct gcaaaaaccg catagccgtt cttaaaaggc tgttggcgga aatacacctc
     3241 aaagaccgcc ttcttattgt gaatcccttg gtcaaagtaa acctgcatca tgttgatctg
     3301 gtacaagtcc gtgtgcaatg tcaaactatc atctggatac atacttttcc tacttcctta
     3361 gctagaaacc catgaaaatt ttcaagaact ttcatgtatt ccaataaatt agtactatta
     3421 tatcacattt tagctggatt gagaaaagag taacaagcta ttctccactc tccaattcat
     3481 ccatatcttg ttcaaatttt ttctgagccc attcgccata gctcttaaga ccaagattgc
     3541 caataaagac ccacggaagg taaatgacat aagtaatgac ccaagcagac aggtatttaa
     3601 aattcaaagg attgtgctga taaatttcta tgttgaattg ataattctgc aacatcaaaa
     3661 gagccgtaat agccaaggtt aggaaaaaac aacccaaaat cgtaaaatga aaacgactat
     3721 agtaggtcac tcccagataa cgggcacgat tgaaaaagta aaatgtccct atgatgataa
     3781 cgattagcag catattagaa ttaaaaaggc ttggtgctaa tactgaaatg atataagata
     3841 ggagcgacaa agcaatgcag atatagaaac tttcagagcc cgctttattg aacagttgtt
     3901 cttctctttc gtctagtaat tgataataat aaaatctatt tttcatcttc ttcctcccaa
     3961 aatagttggt ctagggtttt ccctaaacat ctgcaaatag actggcagag cgagagactg
     4021 ggattgtatt ttcccgcctc tatcaaacca atagtctggc gtgtcacccc gacagcctct
     4081 gccagttgac cttgtgttaa atcacgctct acccgagcta attttaattt taaattttta
     4141 gccaccttcg tcctccttat agttttaata ctcatctact cttaaaaaat ccaaaaccaa
     4201 cacaagctat cagctattta ttataacccc attaatttgc actccagtat tatcttcagc
     4261 cgaaaatcag tattttaggt tgtttgaaat aaacttccta gtttactctt ttatttttat
     4321 ttagtattag ctttagtagg atcttttaaa cgagttttta ttgcttatat ttcttgttta
     4381 gtttagttgg tttcatatca ctattatata atgcttttgg aaatgagcct agtataagtc
     4441 aaaaacgtgt tgaatagtga aagcaggagc ataccaagat gactgatttt ttactgtatt
     4501 ttaggtatgc aaaataatac taaatgaaac taaaatgaat acttcttgct tttcttgaga
     4561 gtgaaaaagt acaataaaat gattgtagtg attagcacaa gatactgact aatagacatc
     4621 gtatttgaag ccacaaatac aggtaaagaa taaaaaggaa gaagttcaat tgctttgata
     4681 aacaaatcat tactagtatt catcatcatc gtatttaata tattagggat aaagagtagt
     4741 aaagctacta aaataccaaa taccaagcca cttttcaatt tgtataaata gaaaatgtaa
     4801 gataatagaa aataaaacag gactaacaaa attgtattga ctacaatagc tactataaaa
     4861 tctaatccta aatcacctgg aatacccaat ttacttacta tgactagtaa cacaatgcca
     4921 ttcaaagcat aaataatgga aactgagatg gcatatagaa aattagacaa gacgtaaatc
     4981 atccgattct tctgttgatt ataaaacaaa cttaccgtat tgtgggaaaa atctctacta
     5041 atgactcgat tagcatgtat aacagccatt aacataccga gcatagcata aaagtttgaa
     5101 atatgcttaa taccaagcga tgtcccttga gtctttagaa taaagaatgt aagcaatatc
     5161 ggcggtataa acgagataag caaactgaga tatattacgc ctg
//
LOCUS       DAVLVZ010000089         5197 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000089 DAVLVZ010000000
VERSION     DAVLVZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.089"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            87..1262
                     /gene="mutY"
                     /locus_tag="VYN77_001871"
     CDS             87..1262
                     /gene="mutY"
                     /locus_tag="VYN77_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1559286.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1317..2021
                     /locus_tag="VYN77_001872"
     CDS             1317..2021
                     /locus_tag="VYN77_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1559287.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2014..3363
                     /gene="vicK"
                     /locus_tag="VYN77_001873"
     CDS             2014..3363
                     /gene="vicK"
                     /locus_tag="VYN77_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1559288.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3365..4174
                     /locus_tag="VYN77_001874"
     CDS             3365..4174
                     /locus_tag="VYN77_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1559289.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4265..4507
                     /locus_tag="VYN77_001875"
     CDS             4265..4507
                     /locus_tag="VYN77_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1559290.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4509..4763
                     /locus_tag="VYN77_001876"
     CDS             4509..4763
                     /locus_tag="VYN77_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1559291.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQINVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4810..>5197
                     /locus_tag="VYN77_001877"
     CDS             4810..>5197
                     /locus_tag="VYN77_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEV1559292.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENVFDLLAILPLNAIFTVFRLGRIFRLVKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLR"
BASE COUNT         1600 a          874 c         1169 g         1554 t
ORIGIN      
        1 tagatagaaa agttatatac tatctattga aaatattata aatcagatac aaagacaggt
       61 gaaaccttct gtgttatact agagatatgt tagatttgaa agaatacggt atcgtcatgt
      121 ggccggagga gaaagtcatt tctttccgtg agaaacttct cgcttggtat gatgaaaaca
      181 aaagagattt gccttggagg agaagtaaaa atccttatca catctgggta tctgaaatca
      241 tgcttcagca gaccagggtg gatacagtta tcccttacta cgaaagattt ttggactggt
      301 ttccaactgt cgaaagtctg gcaactgcgc ctgaggagag tttactgaaa gcttgggagg
      361 gcttgggtta ttattctcga gttcgcaata tgcaggctgc agcccagcag attatgactg
      421 actttggtgg ccaatttcca aatacctatg aaggaatttc cagcttgaaa gggattggac
      481 cttacacagc aggagccatt tccagtattg cttttaactt gcctgagcca gctgtagatg
      541 gtaatgtcat gcgggtcttg gcgcgtctgt ttgaagtcaa ccacgatatt gggattccaa
      601 gtaatcgaaa aatttttcag gcaatgatgg agatcttgat taatccagat cgtccgggtg
      661 attttaatca agccttgatg gacttagggt ctgatattga gtctcctgta aatcccagac
      721 ctgaagaaag cccagtcaag gactttagtg cggcatatca gaatggaacc atggaccgtt
      781 atccaattaa atctcctaag aaaaagccgg ttcctattta tcttaaagcc ttagttgtga
      841 aaaattctca aggacaattt ttacttgaaa aaaatgaaag cgaaaagctc ttggcaggtt
      901 tttggcattt cccctttata gaagttgata acttttcgca agaagagcag tttgacctct
      961 ttcatcaggt tgcggaagaa agtgtgaact ctggccccag tccagaagag agtttccagc
     1021 aggactatga cctagatgtt gattggcttg atgtttgttt tgatacagtc cagcatgttt
     1081 ttagtcatcg aaaatggcat gtacagattg tagcaggtca ggtgagtgat ttccatgatt
     1141 tttcagatag ggaagttcgt tggctttcac cagaagaatt caagaattac ccacttgcca
     1201 aaccccaaca aaaaatctgg caggcttatg cacaagccaa cttagacagt agccaagact
     1261 agctattgtg tcttctgact attttttggt ataatagtag agaaaaaggt gaacatatga
     1321 aaaaaatact aattgtagat gatgagaaac caatctcgga tattatcaag tttaatatga
     1381 ccaaggaagg ttacgaagtt gtaactgctt ttaatggtcg tgaagcgcta gagcaatttg
     1441 aagcagagca accagatatt attattctgg atttgatgct tccagaaatt gatggtttag
     1501 aagttgctaa gaccattcgt aagacaagca gtgtgcccat tcttatgctt tcagccaaag
     1561 atagtgaatt tgataaggtt atcggtttgg aacttggggc agatgactat gtaacaaaac
     1621 ccttctccaa tcgtgagttg caggcgcgtg ttaaagctct tctgcgtcgt tctcaaccta
     1681 tgccagtaga tggtcaggaa gcagatagta aacctcaacc tatccaaatt ggggatttag
     1741 aaattgttcc agacgcctac gtggctaaaa aatatggcga agaactagac ttaacccatc
     1801 gtgaatttga gcttttgtat catttagcat cgcatacagg tcaagtcatc acgcgcgaac
     1861 acttgcttga gactgtctgg ggttatgact attttggtga tgtccgtaca gttgatgtga
     1921 ctgtacgacg tctgcgtgag aagattgaag atacgcccag ccgaccagag tatatcttga
     1981 cgcgccgtgg tgtagggtat tacatgagaa ataatgcttg atttactgaa acaaaccatt
     2041 tttaccagag attttatctt tatcctgatt ttgttaggtt tcatccttgt tgtgaccctc
     2101 ttattactgg aaaatagacg tgataatatt cagttgaagc aaatcaatca aaaggttaaa
     2161 gatttgattg caggagatta ttccaaggtt cttgatatgc aaggtgggtc tgaaatcacc
     2221 aatattacca ataatttgaa tgacttgtcg gaggttattc gtctcactca ggaaaatcta
     2281 gaacaagaga gtaagaggct aaatagtatt ctgttttata tgacagatgg ggttcttgcg
     2341 accaaccgtc ggggtcagat tatcatgatt aacgatacag ccaagaagca actggggttg
     2401 gttaaggaag atgttctgaa tagaagcatt ttggaattgc tcaagataga agaaaactat
     2461 gaattgcgtg atttgattac ccaaagtcca gaattgttgc tagattccca agatatcaat
     2521 ggcgaatatt tgaaccttcg agttcgcttt gccttgatac gtcgagagtc tggctttatt
     2581 tcaggtttgg tggctgtttt gcatgatacg acggagcagg agaaggaaga acgcgaacga
     2641 agactctttg tttccaatgt tagccatgag ttacggactc ctctgactag cgtaaaatcc
     2701 tatcttgaag ccttggatga gggggctttg tgtgaaactg tagcaccaga ctttatcaag
     2761 gtttctcttg atgagaccaa ccgtatgatg cgcatggtga cggatctcct ccatctttca
     2821 cgtattgata atgctaccag tcacctagat gtggaactga ttaacttcac tgcttttatt
     2881 acctttatcc tcaatcgttt tgacaagatg aaaggacagg aaaaggagaa aaaatatgag
     2941 ttggtgagag attatcccat caattctatc tggatggaaa ttgatacaga taagatgacg
     3001 caggttgtcg acaatatttt aaataatgct attaagtatt cgccagatgg gggtaaaatc
     3061 actgtcagaa tgaagacaac tgaagaccag atgattttat ccatttctga ccacggtttg
     3121 gggattccta agcaggattt accacgtatc tttgaccgtt tctatcgtgt ggatcgtgct
     3181 agaagtcgtg cacaaggtgg tacaggtcta ggactgtcta tcgctaaaga aattatcaaa
     3241 caacataagg gctttatttg ggccaagagt gaatacggca agggttcaac ctttaccatt
     3301 gtgctccctt atgataagga tgcagtgaaa gaagaagtat gggaggatga agtagaagac
     3361 tagaatgagt gaaataggct ttaaatacag tattttagcg tcgggttcca gtggaaattc
     3421 tttttatctg gaaacctcaa aaaagaagct tttagtagat gcaggcttgt ctggcaagaa
     3481 aattaccagt ctgctagctg aaattaaccg taagccagaa gacctggatg ccatcttgat
     3541 tacccatgag cattcagatc atatccatgg agtaggcgtt ttggctcgca agtatggtat
     3601 ggatctttat gccaatgaaa agacctggca agctatggaa aatagtaaat atcttggcaa
     3661 ggtggattct tcgcaaaagc atatttttga aatgggtaaa accaaaacct ttggagatat
     3721 cgacatcgag agttttggtg taagccatga tgcagtcgca ccgcagttct atcgctttat
     3781 gaaggatgat aagagttttg tccttttgac agatacaggt tatgtcagtg accgtatggc
     3841 gggcattgtc gaaaatgcgg atggctatct tatcgaggcc aaccatgatg tagagatttt
     3901 gcgatcaggt tcttacgctt ggcgactcaa acaacgaatc ctatctgacc ttggtcacct
     3961 ttctaacgag gacggtgctg aagctatgat tcggacgcta ggaaatcgta ctaagaagat
     4021 ttaccttggg catttatcta aggaaaacaa tatcaaggaa ctggctcata tgaccatggt
     4081 caatcagctg gctcaagctg atctgggagt cggagtagac tttaaggttt atgatacctc
     4141 accagatacc gcaacaccat tgacagagat atagaaagaa cgctgagaag gtgttctttt
     4201 tatattgact gaacacctaa aaagtaatac aatggtgtta ccattaaaaa agggagcaca
     4261 aaagatgact actattacat taaaagtttc tgaagctgat aaaacattta tgaaagcaat
     4321 ggctaagttt gaaggagttt ccctttcgga acttattcgc accaaaactc ttgaagccct
     4381 agaagatgaa tacgatgctc gtgtggcaga tttagcctat caagagtatt tagaagactt
     4441 ggaaaaagga gttgaaccca ttacttggga agaaatgatg catgatttag gcttgaagga
     4501 tgaataattt gtataaatta gttccaacaa gacgtttcat caagcaattg aaaaaattgg
     4561 accgttatac gcagaagcta attacaaact atttacaaat caatgttttg gaagacccaa
     4621 gacgacacgg aaaggctttg gttggtaatc gcgttggtca atggcgctat agaattggta
     4681 attatcgagt tatcgtacaa attgtagatg atgaattagt tgttgctact ctagaagttg
     4741 gtcatcggag agatatttat tgaattaaag ctgagtaata acaatataga aagtaaggag
     4801 agtaaaaatt tgaagaaaaa atggtttttt gcagattatt atgatacaac aattatttta
     4861 cttgctttaa tctctgttat tctggtatta cttggatttg ctgagatgat tgatttggac
     4921 aatccgcctt atagtattat tgatttagta atctggggtg tttttgtaat tgattatagt
     4981 tggcgctttt ttattactaa aagaaaatgg cgctttattc ttgaaaatgt ttttgattta
     5041 ttggctattc tccctttaaa tgctattttc acagtttttc gattagggcg tatctttcgt
     5101 ttagttaaac taacaaaatt actgaaactg actcgtttac ttagaataat tggacttaca
     5161 ggtaaattag aaagaaaaat tagtagattc ttacgga
//
LOCUS       DAVLVZ010000090         4991 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000090 DAVLVZ010000000
VERSION     DAVLVZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.090"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..360
                     /locus_tag="VYN77_001878"
                     /pseudo
     CDS             1..360
                     /locus_tag="VYN77_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ion transporter"
     gene            complement(623..1609)
                     /locus_tag="VYN77_001879"
     CDS             complement(623..1609)
                     /locus_tag="VYN77_001879"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1559293.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1803..4271
                     /gene="gyrA"
                     /locus_tag="VYN77_001880"
     CDS             1803..4271
                     /gene="gyrA"
                     /locus_tag="VYN77_001880"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1559294.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            4271..>4991
                     /gene="srtA"
                     /locus_tag="VYN77_001881"
     CDS             4271..>4991
                     /gene="srtA"
                     /locus_tag="VYN77_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1559295.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFN"
BASE COUNT         1547 a          853 c         1115 g         1476 t
ORIGIN      
        1 ttaatatctt tattattcta gtaggaagtt caattttatc tgtagttgaa gaaaaatcct
       61 tctcagatag tctttgttgg gctcttgtga cagtaactac tgttggttat ggtgatattg
      121 ttccagtttc ccttttgggg aaatggctag cagttttact gatgcttgtc ggtattggaa
      181 caatagggat gttaacaagt gctttgacga actttttttg taaaggataa tccagatgaa
      241 cagataaaac ttgataaact ccaagatgaa ttatctagtc cgagaatatt actagagaaa
      301 caatctaaaa agatagaaga actacataaa atgatacaag atttgattga aaaaacataa
      361 taaaaataaa gatgagaaaa agtttgaact gaggagaaat caagtaagtt ttgacacaat
      421 agaacgcata ttatcaggta taaaaacttg atactatgcg ttttattgtg ggaagatgta
      481 taatggattg aaataagata tgaacaaatc gattaggaat ttaaagcatt ttataacaac
      541 gttttagagt aacggggtac tatttcatat aatacaggaa aaaacaactc cctgatgact
      601 caaggagttg tctatagtta aattagtttt tagaagcttc ttggaattct ggatttttcc
      661 atgcttcgtc aataatagct tgcaattctt tagcagatgc ttgcattttt tgagtttctg
      721 catcgttcaa tggaatgttt actggacgaa cgataccatg tgcaccaaca acagctggtt
      781 gaccgataaa gacattctca actccgtatt gaccttcttg gaatactgaa agtggaagta
      841 ctgcgttttc atcgtcaagg attgctttag tgatacgagc aagggctact gcgataccgt
      901 agtatgttgc accttttttg ttgatgattg tgtaggctgc atcacgaaca ccttcgaaca
      961 attcaatcaa ttcagcttct tgaacatttt gagtgtcttt aaggaattct tcaaggttta
     1021 caccagcgat gttagcgtgt gaccaaacag cgaactcaga gtcaccgtgt tcacccatga
     1081 tgtaggcgtg cactgaacga gcatccacat ccaatttttc agcaagtgct tgacggaaac
     1141 gagctgagtc aagtgaagta cctgaaccga taacgcgttc tttagggaaa ccagagaatt
     1201 tccaagttga gtaagtcaaa acgtcaactg ggttagcagc aacaaggaag atacctttga
     1261 aaccagattc aacaacttga gttacgattg atttgttgat agcaaggttt ttacctacaa
     1321 ggtcaagacg agtttcacct ggtttttgag gtgcacctgc agtgatcaca acaaggtcag
     1381 cgtctgcaca gtcagagtat tgagctgcat agattttttt aggtgaagtg aaggcaaggg
     1441 cgtgactaag gtcaagcgca tcaccaacag ctttttcatg caattgtgga atttcgataa
     1501 ttccaagctc ttgtgcaatt ccttggttaa caagtgcaaa agcgtaagat gaacctacag
     1561 caccatcacc gacaaggata acttttttgt gttgtttagt tgaagtcatt gttttaaaca
     1621 tctccttaat tttattaggg gattttccct agacaacttc attctatcac ttttaaaaaa
     1681 ctttgtcacg aatatgcctt atagttctcg atgtaaacgt tttagtggtt tagaggctga
     1741 aatagatggg aatttatggt ataatgttgt tacttactaa ttgtgaaatg aggcatttat
     1801 taatgcagga taaaaattta gtgaatgtca atctgacaaa ggagatgaag gcaagtttta
     1861 tcgactacgc catgagtgtt atcgtagcgc gagctcttcc tgatgttcga gatggcttaa
     1921 aacctgttca ccgtcgcatt ctctacggaa tgaatgaatt gggtgtgacc ccagacaaac
     1981 cccataaaaa atctgctcgt attacagggg atgtcatggg taaatatcac ccacacgggg
     2041 attcctctat ttatgaagcc atggtccgta tggctcaatg gtggagctac cgttacatgc
     2101 ttgtagatgg tcatgggaat tttggttcca tggatggaga tagtgctgcc gctcaacgtt
     2161 ataccgaggc acgtatgagc aagattgctc tggaaatgct tcgtgatatc aacaaaaata
     2221 cagttgattt cgttgataac tatgatgcca atgaacggga acccttggtc ttgccagcgc
     2281 gttttccaaa ccttttggtt aatggagcaa ctggtatcgc ggttgggatg gcaaccaata
     2341 ttccacctca taatctgggt gaaaccattg atgcagtgaa gttggtcatg gataatcctg
     2401 aagtgactac caaggacttg atggaagtct tgcctggacc agattttcca actggtgctc
     2461 ttgtcatggg gaaatcaggt atccataagg cttatgaaac aggtaaaggt tcgattgtcc
     2521 tacgttctcg tacagagatt gaaacgacta agactggtcg tgagcgtatc gttgtaacag
     2581 aatttcctta catggtcaat aaaaccaagg tgcatgagca tattgttcgc ttggttcagg
     2641 aaaaacgcat tgagggtatc acagcagtac gtgatgagtc aaaccgtgaa ggtgttcgat
     2701 ttgttattga agtcaagcgc gacgcctcag ccaatgttat tctcaataac ctcttcaaaa
     2761 tgacccaaat gcaaaccaat tttggtttca atatgctcgc tatccaaaat ggtataccga
     2821 aaattttgtc tcttcgtcag attttggatg cttatatcga gcaccaaaaa gaagtggttg
     2881 ttcgtcgtac acgttttgat aaggaaaaag cggaagcgcg cgctcatatc ttagaaggtc
     2941 tcttgattgc gctagaccat atcgacgaag tgattcgtat catccgtgct agtgaaacgg
     3001 atgcggaagc tcaagctgag ttgatgagca agtttaagct ttctgaacgt caaagtcaag
     3061 ctatccttga tatgcgtctt cgtcgtttga caggtttgga acgcgataag attcaatctg
     3121 agtatgatga cctcttggct ctgattgcgg atttagcaga tattcttgct aagcctgaac
     3181 gtgtttctca aattatcaaa gacgaattgg atgaagttaa acgtaaattt tctgataagc
     3241 gccgtacaga gttgatgatt ggacaggtct tgagtctcga ggatgaggac ttgattgaag
     3301 aatcggatgt cttgattacc ctttctaaca gaggctacat taagcgtttg gatcaggacg
     3361 agttcactgc tcaaaaacgt gggggtcgtg gtgtccaagg aacgggagtg aaagatgatg
     3421 actttgttcg tgagttagtg tcaactagca cccatgatca tctgctcttc ttcacaaaca
     3481 agggacgtgt ctatcgtctt aaaggttatg aaattcctga gtatggtcgg actgccaaag
     3541 ggctaccagt agtcaatctc ttgaaattgg atgaagacga aagtattcag acggttatca
     3601 atgttgagtc tgatcgcagt gatgatgctt atctcttctt tacaacccgt cacggtattg
     3661 tgaagagaac cagtgttaag gagtttgcca atattcgtca aaatggtctc aaagcgctga
     3721 atttaaagga tgaagatgag ttaatcaatg tcttgttggc agaaggagat atggatatta
     3781 tcattggtac caagtttggt tatgcagttc gctttaatca atcagccgtt cgtggtatga
     3841 gtcgtatcgc cactggtgtg aaaggtgtta accttcgtga aggagacaca gttgttggtg
     3901 ccagcttgat tactgatcaa gatgaggttc ttattatcac agaaaaagga tatggtaagc
     3961 gtacagtcgc tactgaatac ccaacaaaag gtcgtggtgg taagggaatg cagacagcta
     4021 aaattaccga aaaaaatggc ttgctggccg gtcttatgac tgttcaaggg gatgaggatt
     4081 tgatgattat cactgataca ggtgtcatga ttcgaaccaa tcttgccaat atttcacaaa
     4141 caggacgtgc aactatggga gttaaagtaa tgcgcctgga tcaagatgct cagatagtga
     4201 ctttcacaac ggttgcggtg gcagaaaaag aagaagttgg gacagaaaac gaaacagaag
     4261 gtgaagcata atgtctcaaa aaaataataa aaagaaaaac aagcgaaaaa atctgctgac
     4321 aaatatccta gcaggatttc tgatattact gtcactggct ttgattttta atactcaaat
     4381 tcgaaatatt ttcatagtct ggaataccaa taagtatcaa gttagccagg tatcaaaaga
     4441 aaaattagaa gaaaatcagg atacagaagg caattttgac tttgattctg tcaaagctat
     4501 ctcttcggaa gctgttctaa cttctcaatg ggatgctcaa aaattaccag ttattggggg
     4561 aattgcaatt cctgaattgg aaatgaattt gccgattttt aaaggacttg ataatgttaa
     4621 tctcttctac ggagctggta caatgaaacg cgagcaagta atgggagaag gaaattatag
     4681 tctagctagt caccatatct ttggtgttga taatgctaat aaaatgttat tttctccttt
     4741 agataatgct aaaaatggca tgaagattta tctaaccgat aaaaataaag tttatactta
     4801 tgaaatacgt gaagtcaaac gtgtgacacc ggatcgtgtt gatgaagttg atgatagaga
     4861 tggggtcaat gaaatcacat tagtaacctg tgaagacctt gctgctacag aacgtattat
     4921 tgtcaaaggt gatttgaaag aaacaaaaga ttattcacaa acatctaatg aaatcctaac
     4981 agctttcaat c
//
LOCUS       DAVLVZ010000091         4938 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000091 DAVLVZ010000000
VERSION     DAVLVZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.091"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..460
                     /locus_tag="VYN77_001882"
     CDS             <1..460
                     /locus_tag="VYN77_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1559296.1"
                     /translation="LLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHALIYYIE
                     SLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDEALFRFS
                     ILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(626..697)
                     /locus_tag="VYN77_001883"
     tRNA            complement(626..697)
                     /locus_tag="VYN77_001883"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(662..664),aa:Arg,seq:cct)
     gene            complement(739..1086)
                     /gene="rplS"
                     /locus_tag="VYN77_001884"
     CDS             complement(739..1086)
                     /gene="rplS"
                     /locus_tag="VYN77_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1559297.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(1205..1534)
                     /gene="crcB"
                     /locus_tag="VYN77_001885"
     CDS             complement(1205..1534)
                     /gene="crcB"
                     /locus_tag="VYN77_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1559298.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1528..1902)
                     /gene="crcB"
                     /locus_tag="VYN77_001886"
     CDS             complement(1528..1902)
                     /gene="crcB"
                     /locus_tag="VYN77_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1559299.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(1899..2165)
                     /locus_tag="VYN77_001887"
     CDS             complement(1899..2165)
                     /locus_tag="VYN77_001887"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1559300.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(2281..2724)
                     /locus_tag="VYN77_001888"
     CDS             complement(2281..2724)
                     /locus_tag="VYN77_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1559301.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSIGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVV"
     gene            2828..3763
                     /locus_tag="VYN77_001889"
     CDS             2828..3763
                     /locus_tag="VYN77_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1559302.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3817..4101
                     /locus_tag="VYN77_001890"
     CDS             3817..4101
                     /locus_tag="VYN77_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1559303.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(4158..4376)
                     /locus_tag="VYN77_001891"
     CDS             complement(4158..4376)
                     /locus_tag="VYN77_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559304.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETKPTDESGT"
     gene            complement(4373..4938)
                     /locus_tag="VYN77_001892"
                     /pseudo
     CDS             complement(4373..4938)
                     /locus_tag="VYN77_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1557 a         1060 c          867 g         1454 t
ORIGIN      
        1 tctcttaaat aaacaagctc ataggaatac tgtatcagga gattttggat gcttatctaa
       61 cacacgaaag gctcagggaa gacatttgga acaagaaggt aatattaaac atgctttaat
      121 atattacata gaatctttga taattactat ttcaggatta gaaaacaatt tttcagccac
      181 tgattatcca gtatattatc ccgattcgat acctgactac tcactaaaac atattcaaac
      241 attaatggaa tcattatctg atgacgatta tgattttgct tttgatgaag cattatttcg
      301 cttctcaatt ttgaatgcaa atcatttttt atctaaggaa gatattgact atttaagagt
      361 taatttacct cgttccactg ctgaagaaat aaacaattac ttaaagaaat atgaatgtta
      421 tagtccttta aataatttag aacttgacga ttttgaataa attgactata caaacattta
      481 tatactcgat atagtctcaa ttttatctga tgattgccca aatttttcaa taataaaacg
      541 cataatatta tggagacaat cccctatatt atgcgttctt ttaatatcaa agactttttg
      601 acaaacttct tcgatatcta attactgccc cctgcaggaa tcgaacctgc aactactcct
      661 taggagggag ttgttatatc cattgaacta agggagctag ataaaaactc tgctgaaaag
      721 cagagttttt tagtcgaatt aacgacggat ttctttgata cgagctgctt taccttgaag
      781 agcacgcaag tagtacaatt tcgcacgacg tactttaccg taacgaacaa cttcgatttt
      841 ttcaacacgt ggagtgtgga ttgggaagat acgctcaaca cctacaccgt tagagatttt
      901 acgaactgtg tagttttctg agattccagc acctttacgt gcgataacaa caccttcaaa
      961 aatctggata cgttcacggt taccttcgac aactttcgcg tgtacacgaa cagtgtcacc
     1021 aggacggaat gatgggatat ctgtacgaag ttgaccttca gtcaagcttt ggattaatgg
     1081 attcatttta ttctcctatc tttgtcaatc ttgaggaaat ttcctcagcg gataaactgt
     1141 atttttgtgc gtccatcaca cacaagatac agtctaccaa attttcacaa aaaagtaaag
     1201 aaatttatag caaaattcct aaaaaaatcg caaccaaacc acctatgtaa gtcaaagtca
     1261 ggtaagaata aaagaccttc ttatcactta acagtctttg aagttcgtca ttcaaggtcg
     1321 aaaaagttgt taaaccgcca caaaatcctg ttgctagaat agcatatact tccttagact
     1381 ctacatgatt gtagaatact ccaattaaaa aacaacctaa aagattagct atgagcgttc
     1441 cgagtggcaa tttagaagct tgattgtagc gggaaaagaa ataccgtacg agtgctccca
     1501 agccacaggc gattgcaaga tagacgatta ccatttcttc ctccctagaa aataagctaa
     1561 gagtaggcct ccaccgatgc tcaaaagcaa atacaggact aaactaagat aacgccctgt
     1621 atcaagcagc ttcacagtat caagcattag gctggaaaaa gttgttaaac ctccgcaaaa
     1681 acccgtcccc agtgctaaaa tcaagccctt actagtcccc ttatagacca gatagccctt
     1741 gacaagaaaa atcaagcaga aaattcccag atagttgaca aagagagtgc cccaaggaaa
     1801 gtctggactg gctggtaacc aggtggaaac tagatatcgg acaagtccgc ccaacatagc
     1861 agctagaaaa attcctagcg gataaaattg ttcttttttc atttgatatt ttgatcctga
     1921 taatcacgcg aacgtttgag tatgtccgaa aaagtcgcta caatagtctc ctgatagcgc
     1981 ttgtcctcta cacgactcct aaccttttca aaaataactt cttctctctt agtatctaaa
     2041 atcggtttac ctgaagcttt cttataagca accaccccct caactaaatg cattcgttct
     2101 tctaacagtt tgacaatttg gtcatcgatt tgatcgattt cttgacgaat aatatctaaa
     2161 tccatacagt ctcctccttt atttgaatta ttgtatcaaa aagccatcaa ttaggctagt
     2221 aaaatcccaa ctctcgataa gaaaaatgca ctgattgatt acatcagcgc acttttatac
     2281 ttatactact ttagcaacca attcttctgc gaattgttcc aagcgttcaa tatcttcttc
     2341 ctcggcagaa agatcaactt taacacactc tgaacctttt tctgctccta ttgacacaaa
     2401 gacacggtca aagtcatcaa cagccttaca gaattcatcg tagaaggtat ctcctgagcc
     2461 gaccactccg tagattttgc cattcaagtt gagatctgct aggtcttcgt agaagtccat
     2521 catctcatct ggcaattcac catcaccata agtataggtc gcaacgatag cgatgtctgc
     2581 ttccaagaag tctgaagcgt caacagttgt acattcatca acatcaacat ccaagcccaa
     2641 gtcacgtaat ttgtctgcta caatatctgc aatttcttcg gtattaccgg tcatactggc
     2701 aaatacaatt tttgctaatg ccatatcgtc ctcctcaatt tatctttctc cattatatca
     2761 catctttttc attttaaaaa taaaaattct tgtgctacaa tgaaatgaga aagaattgag
     2821 gttatttatg gagatttgcc aacaaatttt agaaaaaatt aaagaatacg acaccattat
     2881 cattcatcgt catatgaaac cagaccctga tgccttggga agtcaggtgg gattgaaagc
     2941 cttgctggaa catcatttcc cagaaaaaac catcaaagcc gtcggttttg atgaaccaac
     3001 tcttacttgg atggctgaga tggatcttgt tgaagataga gcctaccaag gcgcacttgt
     3061 catcgtctgt gatacagcta atactgctcg tatcgatgat aagcgctata gtcaaggtga
     3121 ttttctcatt aagattgacc accatccaaa tgatgatgta tacggtgacc tgtcttgggt
     3181 cgatactagt tcaagtagcg ctagcgagat gattacccta tttgcccaaa caacccaact
     3241 agccttggca gatcgcgatg ctgagttgct ctttgcagga attgtcggtg atacaggtcg
     3301 cttcctctac ccttctacca ctgcacggac tcttcgcctg gctgcttatt tgagagaaca
     3361 taactttgac tttgcggctc tcactcgcaa aatggacact atgagctaca aaattgctaa
     3421 actgcaaggc tacatctacg accatctgga agtggatgaa aatggtgctg ctcgcgttat
     3481 cctgagtcaa gaagtcttga aacaatacaa tgtaaccgat gctgaaactg cagccattgt
     3541 cggtgcacct ggacgcattg acagagtgag tctctgggga atttttgtcg aacaggctga
     3601 tggccactac cgagttcgct tacgcagtaa aatccatcct atcaatgaaa tcgccaagga
     3661 gcatgatggt ggaggccacc ctctagcaag tggtgctaat tcctatagcc tagaagaaaa
     3721 cgaaatcatc taccaaaagt taaaaaactt gcttaaaaac tgataaaata cttgccaaac
     3781 ttttcagaat ctgatagact agtatggtaa caatctatgg ctcgcaaaga gaccatggca
     3841 gaaaggaaat attgcaaaat gaaaaaagat atccatccag aatatcgccc agttgtcttc
     3901 atggacacaa ctactggtta ccaattcctt agcggttcaa caaaacgctc taacgaaaca
     3961 gttgagttcg aaggcgaaac ttacccattg atccgtgtgg aaatttcatc agactcacac
     4021 ccattctaca ctggacgtca aaagttcact caagcagatg gacgcgtgga tcgtttcaac
     4081 aaaaaatacg gtctcaaata atgataagag aacagtttcg gctgttcttt tttgtttctt
     4141 gaaatcaact gctgttttca tgttccagac tcatctgtag gtttggtttc catgctacta
     4201 ggcaggaagg aaatagctgt ttcaacacgt ccataatgag ctgtactatt gtcacgaacc
     4261 acactttcat tgatggtcca agtggaattc attttcttaa aagcttctcg gactttttcc
     4321 aaatctttgg aggcaatggc ctgctctaag gtttcaaaac gaggacttat actcatctgc
     4381 tttcacaaag cattctagtc catctccgat taacgatggg ctttatcacc tccttctcca
     4441 gtccttgtat gatatcttaa agttgattca tgacatcttc caaagtttga aaggctttat
     4501 ttttaaatcc acgtttacga atctctttcc acacttgttc aatggggttc atctctggtg
     4561 tgtacggagg aataaatgca aagccaatat tagtcggaat ctttaaggta cttgatttat
     4621 gccatatagc attgtccata acgagtaaaa gataatcatc tggataagct tgtgaaagct
     4681 cttctaaaaa ggcgttcatc cactcagtat tacatccacc agctcttaag aaaaatgatt
     4741 cgcctgtatg ggcatcaaca gctccataac aatagcgaaa ttctcgtata tagtgactat
     4801 ggacatgtgg acctactcct attggagacc aacaagatcc cagtttactg attctaccga
     4861 aaccagcctc atcttggtac atcgagcgaa ccttatgaaa aggtctactg gttttaaagc
     4921 gctttctttt cttcttga
//
LOCUS       DAVLVZ010000092         4901 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000092 DAVLVZ010000000
VERSION     DAVLVZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.092"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            75..671
                     /locus_tag="VYN77_001893"
                     /pseudo
     CDS             75..671
                     /locus_tag="VYN77_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            747..1196
                     /locus_tag="VYN77_001894"
                     /pseudo
     CDS             747..1196
                     /locus_tag="VYN77_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            1209..2300
                     /locus_tag="VYN77_001895"
     CDS             1209..2300
                     /locus_tag="VYN77_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559305.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQTIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKDNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            2290..3935
                     /locus_tag="VYN77_001896"
                     /pseudo
     CDS             2290..3935
                     /locus_tag="VYN77_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4038..4730)
                     /locus_tag="VYN77_001897"
     CDS             complement(4038..4730)
                     /locus_tag="VYN77_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1559306.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1541 a         1045 c          841 g         1474 t
ORIGIN      
        1 aatagaattg taggttttaa aaacgcttac attgttttaa aataaactta aataacacaa
       61 cggaggtatc catcatgaaa aagaaatgga tgtattatgc tgcttgttct tctaatgaat
      121 ctgccgatga cagttcatct gataaaggag acggcggttc gctagtcgtt tattgaccaa
      181 actcagaggg cttaattgga gcaactattc ctgcctttga agaaaaatat ggtatcaaaa
      241 tagaactgat tcaagctggt actggagaac ttttcaaaaa actagagtca gaaaaagaag
      301 ttcctgtagc tgatattatc tttggtggtt cttatacaca atatgctacc cacggagaac
      361 tctttgaaaa ctatatttca aaagaaaatg ataatgttat caaagaatat caaaacacaa
      421 ctggctactc tactccttat acactagatg gtagtgtttt aatcgtcaac cctgatttaa
      481 ctaaaggcat gaacatcgaa ggatataacg atcttctcaa acctgaacta aaaggaaaaa
      541 tcgcaactgc tgacccagca aactcttcta gcgcctttgc tcaattaaca aatatgctac
      601 aagctcaagg tggttacaaa gatgataagg cttggtctta tgtaaaagat cttttcacac
      661 ttattgatgg catagtgaaa tgaaataaga acaggacaaa ttaatcagga cagtcaaatc
      721 gatttctaac aatgttttag aagtagatgt gtactattct agtttcaata tactataaaa
      781 tcggttcaag ttcatctggt gtctataaag cagtcgctga tggagaaatg actgttggtc
      841 tctcttatga agatccagca gttaaactct taaatgacgg agctaacatt aaggtagtct
      901 atccaaaaga aggaaccgtc ttcctacctg ctagtgctgc tatcgttaaa aaatctaaaa
      961 atatggaaaa tgccaagaaa tttatcgatt ttattatctc tcaagaagta caagatacac
     1021 ttggtacaac cactactaac cgtcctgttc gtaaaaatgc taaaacaagc gaaaacatga
     1081 aaccaattga caaaatcaaa acactcactt aagattatga ttatgtcatc aagaataaat
     1141 cagatatcgt taagaaatac aacgaagtct ttacagatat ccaatctaaa cagtaaaaga
     1201 ggttcactat gagtgagatc gaaattatta acgccaaaaa aatctaccac gatgtccctg
     1261 ttattgagaa tttgaacatt acaattccaa aaggaagtct ctttaccctt cttggagctt
     1321 caggatgtgg gaaaacgacc cttcttcgta tgattgcagg tttcaacagt atcaaagatg
     1381 gagaatttta cttcgatgat acaaaaatca ataatatgga acccagcaaa cgcaatatcg
     1441 ggctggtttt ccaaaactac gctattttcc cacatttgac tgtccgagac aacgttgctt
     1501 ttggtcttat gcaaaagaag gttccaaaag aagaattgat tcaacagacc aacaagtatc
     1561 ttgaactcat gcaaattgct caatatgcgg atcgaaagcc cgataaactc agtggtggac
     1621 aacaacaacg tgtcaccttg gcatgcgcct tagcggttaa tccaagtgtt ctcctcatgg
     1681 acgagccact tagtaatctg gaggccaaac ttcgcttgga tatgcgtcaa accatccgag
     1741 aaatccaaca cgaagtggga attacaactg tttatgtaac ccacgaccaa gaagaagcca
     1801 tggctatttc agaccaaatt gctgttatga aagatggggt gatccaacaa atcggccgac
     1861 caaaagaact ctatcataaa ccagctaatg agtttgtggc aacctttatc ggacgcacaa
     1921 atattatccc tgccaatctt gaaaaacgga gcgacggcgc ttatatcgtc ttttcagatg
     1981 gctatgccct tcgaatgcta gctcttgatc aggttgagga gcaagctatt catgtaagca
     2041 ttcgtcccga agagtttatc aaagatgaat ctggagatat cgaaggaact attagcgata
     2101 gcgtctatct tggactaaat acggagtatt tcattgagac aggttttgcc tcaaaaattc
     2161 aagttagtga agaatcaact tttgaagaag atctacaaaa agacaatcgt attcgtctac
     2221 gaatcaatac gcaaaaatta aacatctttt ctgcagatgg ttcccaaaac ctgataaaag
     2281 gagtcaacca tggaacgtaa aaaactaaat atttggacag cctcctcttt cttcatcttt
     2341 cttacctatc ttgtctttct cgtttatcct atcgttaccg tgctcaagta agcacttata
     2401 catgaaggat aattctcact agctaatttt gtcactttct ttagtaaaac ctactactct
     2461 gagacactag tcaacagttt cagggtttcc attaccgcta ctgtcacttc cttagttgta
     2521 ggaaccctat tagcttatct cttctctatg tatgacttca aggggaagaa atttctacaa
     2581 atattgatta tcattgcttc catgtcagct cctttcgtag gagcctactc ctggattctc
     2641 ttgctaggac gaaatggggt catcactaaa tttttgacaa atgcccttca tcttccagct
     2701 atcgatattt atggattcaa aggaattata cttgtcttta cactgcaact attcccactg
     2761 gtatttctat atgttgctgg aacaatgaac agtattgaca attctctact tgaagcagct
     2821 gaaagcatgg ggtccttcgg atttaaacct atcgtaacgg ttgttttacc tctcctagtt
     2881 ccaaccttac tagcagctcc ttgcttgtat ttatgagagc attctcagac tttggaacgc
     2941 ctatgttgat tggcgaagga tatcggactt tccctgtcct gatttatacc caatttatta
     3001 gcgaggttgg aggaaattct gcttttgcaa ttatggcgat tatcattgcc ttggcaattt
     3061 tccttatcca aaaacacatt gcaaaccgct acagtttcag catgaatccg ctccatccaa
     3121 ttgagcctaa aaaaactaca aaaggaaaaa tggctgccat ttatgcaaca gtctacggaa
     3181 ttatctttat ctatatttta cctcaaatct acttaattta tacctctttc ctaaaaacat
     3241 caggtatggt atttgttaaa ggttattctc caaacagtta caagctagct ttcaatcgta
     3301 tgggatctgc tattttcaat accattcgta tccctttgat tgccttagtt ctagttgttc
     3361 tatttgcgac atttatctcc tacctagccg ttagaaaacg gaatttgttt acaaacttaa
     3421 ttgacagcct cagtatggta ccttatattg taccaggaac cgttctaggg attgccttca
     3481 tttcttcctt caatactggt ctatttggaa gtggatttct tatgattaca gggactgctt
     3541 tcatcttgat tatgtctcta tctgtcagaa gattacctta tactattcgc tcatctgttg
     3601 ctagcttaca acaaatagca ccaagtattg aagaagctgc tgaaagctta ggaagtagtc
     3661 gtctcaatac ctttgctaag attacaactc caatgatgct atctggtatc atttctggag
     3721 ccatcttatc ttgggtcaca atgatttcaa aactctctac ttctatcctc ctctacaatg
     3781 tcaaaacaag aacaatgact gtagctattt atacagaggt tctcagagga aattacggtg
     3841 tagccgcagc cttgtcaact atcctgactg ttctaacagt aggttccttg ctcttgttta
     3901 tgaaaatctc taaaagcaat agcattacac tttagttttc cacatcaaaa acagccgaaa
     3961 ggagtaccct cggctgtttt ttcttatctt gatattctta tcaaatccct agctcatggc
     4021 aagcaagtct agactgatta aatagagcct tcttccatct tctcacgtcc tagctctcgg
     4081 taactacggt caccgacaac ttcaacccta aactgaccgt cctcatattc aagaatcgtc
     4141 acgctaccat tatctagacc atgcggatgc atgccattaa tcagataaac aatggttcca
     4201 atagtcattc catggctgac aacaagggcg ttacctccac cttgatcttc catttctttt
     4261 gcaatcattt caaagccttc cttgattcgg ccactgagtt tttcccagcc ttcagcccaa
     4321 ccagctgtat cgacctctac caagccctca gccagttcag cataagacaa ttggtgaacg
     4381 tggtccacat taaagatacg aggaataatg cccatgaaaa gatcgccatc ataggctcca
     4441 tcaaaactac cgaaacacca ttctctgata cgcttgtcca tgcgataagg gattttcccc
     4501 tgcaagccaa gttcttcaag gataattccc atggtctgaa tggtacgacc agaatcactc
     4561 gaataagcgc gctcaaactg tagatcagat tctcgcaaac cgattcctaa ctcttgaatc
     4621 cctcgttcac cttcagcagt taagggagta tcgctccaac cttgcgcgcg accaatcgtg
     4681 ttaaacatgg tcttgccatg acgtaccaaa tacaatcgta cttttaccat tttccgtcct
     4741 ccgtttgctt ctattatatc atgaatgata aaataaaagc cacccataca ggcgactttt
     4801 gaaggagatt attatgaaaa agtttaggag ttcattaaat aaagatatta gatgaaaatc
     4861 aaattcaaac taattcagtg ttatctgttt caaataatat t
//
LOCUS       DAVLVZ010000093         4780 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000093 DAVLVZ010000000
VERSION     DAVLVZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.093"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..708
                     /locus_tag="VYN77_001898"
                     /pseudo
     CDS             1..708
                     /locus_tag="VYN77_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(1223..2215)
                     /locus_tag="VYN77_001899"
     CDS             complement(1223..2215)
                     /locus_tag="VYN77_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1559307.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            2375..4129
                     /locus_tag="VYN77_001900"
     CDS             2375..4129
                     /locus_tag="VYN77_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559308.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAALTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     TLLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            4113..>4780
                     /locus_tag="VYN77_001901"
     CDS             4113..>4780
                     /locus_tag="VYN77_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559309.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLY"
BASE COUNT         1731 a          682 c          764 g         1603 t
ORIGIN      
        1 atggaaactc taaaactata tataaggttg ataataatgg taactatatt ctagcaaaag
       61 agaaaaatgg agttcccctt tcagatgttt ggaatatacc atttcttaat ccaaaagcta
      121 aagaaagagt aggttatcct acacaaaaac ctattctgtt attagaacaa attataaaga
      181 ttgctactga taaaaatgat atagttttag acccgttctg tggaagtgga actactttag
      241 tagcctccaa gattttgaat agaaattata tggggattga tttatctgag gaagctatca
      301 atataactca gcaacgtctg gaaaatgtta taaaaacaag ttcaaattta ttgaataaag
      361 gaatcgaagc atatagaacc aaaactgagg aagaggaaaa cattcttaaa ttattacagg
      421 caaaaattgt tcaaagaaat aaaggaattg atggtttttt acctaaacat tttcaaaaaa
      481 aaaccgatac ctataaaaat tcaaaaaaat aatgaatgtc tgaatgagag tatctcttta
      541 ttacagaatg ctataaactc caaaaaactt gattttggag tagttataaa aactcattca
      601 gataatttat tatttgattt tgatacgatt cctgaaaata taatagtagt tgaccatttt
      661 gaacttacaa tcgaaaaatg gttaagtaaa agtcaacagt tactatagtt tgcaaattat
      721 ttacagaaag aacaaaaaat gctctgaaac actgatttca aagcattttt tgttagttta
      781 aaattactcc cattcttctt ttccaaacgt acaatatatc cagaaccatt caaaatacta
      841 gattctattt tttataatat cactaaatcc acctaattat aggacgtttt cagattttta
      901 gtcccgtccc agtcccagta ccagagaaat attgttttaa tataatatct gtttttgtct
      961 tctaagctct taaaagcaaa agaacaagta aagagtcaag acaaggataa aaagtccata
     1021 ttagggcaaa taaaaagctt taagacagat gacaaatcta agtcaaataa gaaagaccat
     1081 agcaaagggg cagagagata aatattggcg gtcttcggac tgcctttatt tttttatcca
     1141 tttttcaaat caaatttatt cagactatat atgcacatat acacttaaat tcatataaaa
     1201 acatggcttg taaaaaatta ctttaatcac aataatcgca tttaaaattg tgatgtttgc
     1261 aagctaaatt acggacttca cttggaagtt ttcccttgta tcttttataa tagatagaaa
     1321 atttgctggc agatgaatat ccaacagatt ctgctatctc ttttataggt agttcagtgt
     1381 ttaaaagaag agtttcagct acattcattc tttttctttg agtgtactct gtaatgcttt
     1441 gacaatattt ttccttaaat aaatttttta atttagtacc actcatttta gatatttttt
     1501 caagcgtgcc ttgatttaca ttcgttgcaa aatgatcatc taagaatctt gctacatctt
     1561 caagtgcttt atcatcatca atttcaattt tatatttttt tctatttaag tatgtgtcaa
     1621 ttactatact tatccattca tttgcctttg ctttaaagaa aaaatcagcg gcaggagcgt
     1681 ccatcttaca atttaatatt tccattgcca ctctttctaa ggcctttgta agtattattt
     1741 gattcggttg aagcaaggtt gaataaaaag attctggatt aatgttaata gatgctaaat
     1801 gtttttctat tagctctttt ttaaaaccaa tggaaacagc aagataacaa caattctcgt
     1861 gtaataaaaa aacaaaatca tcttttatat tatcaaaatc aaaagtacat aaagagtttg
     1921 cggtaatagt ttgatacgga ttaaactttt ctccgtttgc actgacaatg taacttgaat
     1981 aaattgaaac atagtctgac atactataag tgctattttg aactacttcc tctttgatat
     2041 aaaaatcatg tatatcgata atgaagctgc ctccttcata aaaccaatat aggccttctg
     2101 cataagttga attctctttt ttccaacaaa atgtatgacc tgcacttgaa tacttctcat
     2161 tgtttttaca ttcatctact ttcatataat cttttacaaa atcataatat gacataacat
     2221 actatccctt ttagacaata ttccaattag ccttattaat tcaaaactat tgtattagta
     2281 attataacag atgtataata gaaaagcaat gatagatatt atcaattcag cgaatttata
     2341 tctaaaaggg atattaaaga aaggagatat gcttatgaag atttacaaaa aactatttgc
     2401 ttatgtccaa gataagaaat atcttggggt tttggccata attttttctg ctatatctgc
     2461 tgcacttaca gtatatggat attatttaat ctacaaattt ctagataagt taataattaa
     2521 ttcaaactta tccggtgcag agagtatagc attaaaatct gttattacac taacaagtgg
     2581 agcgatattt tattttgtct caggaatgtt ttcacatatc ttgggattta ggcttgaaac
     2641 aaatttaaga aaaaggggaa tcgatggtct ggaaaaagca agttttaggt tctttgactt
     2701 aaatccatct ggtcaaataa gaaagatcat agatgataat gcagcacaaa ctcatcaggt
     2761 ggtagctcac atgattccag atagctctca ggcaatagtc acacctatac ttgtccttgt
     2821 actaggcttt atagtaagta taagggttgg tataactttg cttgctctta ctataattgg
     2881 cggcttaatt ttaggggcaa tgatgggcga gcaagaattt atgaagatat accaagaatc
     2941 cctatctaaa ctaagtgctg aaactgttga gtacgtgaga ggaatgcaag ttgtaaaaat
     3001 atttaaagca aatgtagagt catttaaaag cttttacaag gctataaaag attactcaaa
     3061 gtatgcttat gattattccc tatcttgtaa aaggccttat gttttgtatc aatggttatt
     3121 ttttggactg attgcaattt taattattcc tatagtttat tttatgacta gcttagctag
     3181 cgcaaaggtg attttacttg agcttatcat gattttattt ttatcaggag ttctctttgt
     3241 ttcattcatg agaatgatgt ggtactccat gtatatttct caaggaaatt atgcagtaga
     3301 tactttagag gcgctttacg aagatatgca aaaagacaaa ttagtgcatg gtaatgtcaa
     3361 taattttaaa aactataata tagaatttga gaatgttagc tttgcttata atgataaagc
     3421 tgtcattgaa aatttatcct ttaatttaga agaaggaaag tcctacgcac ttgtcggttc
     3481 atctggatca ggcaaatcaa cagtagcaaa acttatatca ggtttttaca atgttaataa
     3541 aggaagcata aagataggcg ggatagcaat aagtgaatat tctgacgaag ccttaattaa
     3601 agccatttcc tttgtttttc aagattcaaa attatttaag aagagcattt atgataatgt
     3661 agctttagct aataaagatg cgacgaaaga tgacgttatg agagccttaa aattagcagg
     3721 atgcgattta atattagaca aattcccaga aagagaaaat acaatcatag gctcaaaagg
     3781 tgtttattta tccggtggag aaaaacaaag aattgcaatt gctagagcaa ttttaaagga
     3841 ttccaaaatt attattatgg atgaagcatc agcatctatt gacccagata acgagtttga
     3901 attgcaaaaa gcttttaaaa atcttatgaa ggataaaaca gttatcatga ttgcacacag
     3961 gctatctaca attaaagacc ttgatgaaat tattgtcatg gatagtggaa aaattataga
     4021 aagagggtct gacaaagaat taatgtcaaa agatacaagg tataagagcc tgcaagagat
     4081 gtttaacagt gcgaatgaat ggagggtttc aaatgaaaga gttttataaa aaaagatttg
     4141 ctcttacaga tggaggagca agaaatttaa gtaaagcaac actggcttca tttttcgttt
     4201 attgtataaa catgcttcct gccatattac ttatgatttt tgctcaggaa gttttggaaa
     4261 atatgggcaa aagcaatggc ttttatatag tattctcagt tttgactttg atagcaatgt
     4321 atattttgct ttctatcgaa tacgataaat tatataacac aacctatcaa gaaagtgcag
     4381 atttaagaat aaggacagcg gagaatttat caaaattacc tctatcttac ttttctaaac
     4441 atgacatttc cgacatttca caaacaatca tggctgatat tgaaggcata gagcatgcaa
     4501 tgagccactc aataccaaag gtgggcggca tggtactgtt tttcccatta atatctgtaa
     4561 tgatgctagc gggcaatgtc aagatgggtt tagctgtaat tattccatct attttaagct
     4621 ttatatttat acctttatct aaaaaatatc aggttaatgg acagaataga tattatgatg
     4681 tcttaagaaa aaactcagaa agctttcaag aaaatatcga aatgcaaatg gagattaaag
     4741 catataattt atcgaaggat attaaagatg acttatataa
//
LOCUS       DAVLVZ010000094         4742 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000094 DAVLVZ010000000
VERSION     DAVLVZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.094"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(367..1095)
                     /locus_tag="VYN77_001902"
     CDS             complement(367..1095)
                     /locus_tag="VYN77_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1559310.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGTLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1253..2662
                     /locus_tag="VYN77_001903"
     CDS             1253..2662
                     /locus_tag="VYN77_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1559311.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2680..3975
                     /locus_tag="VYN77_001904"
     CDS             2680..3975
                     /locus_tag="VYN77_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1559312.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3980..4288
                     /locus_tag="VYN77_001905"
     CDS             3980..4288
                     /locus_tag="VYN77_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1559313.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4285..4593
                     /locus_tag="VYN77_001906"
     CDS             4285..4593
                     /locus_tag="VYN77_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1559314.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1603 a          808 c          875 g         1456 t
ORIGIN      
        1 aataaattgg gagattgaaa ttcatttcta gcaatttgtt agaagtcaca gtctactatt
       61 ctaacctcag tatactatat gtgttacaag aaaagactca aaaacttctc gtcagtgtaa
      121 gaatatttga aacactattt aaaactaaat aactataaga agtagatcag gtgaacaatt
      181 tcagtaccta ctctataagc tatacgaaga tatttttcaa catgatggat aaaacgatca
      241 ctcttttaaa gccatcttat ttagaaaaga gaaaccattt aagcatagat gggaccttct
      301 tgccctgttt taaatttttc ttaaactaga gtctgtaaaa gattggcttc attttcacat
      361 tttctcctag gaggaatgtc gtaatgcaaa agaattaaat tcaaataaat catagcgatg
      421 acgactaatc gagtactcaa acggagttcc gttatccaaa taagccactt gttctacttc
      481 aaataccggt tccgttggaa ggagctgcaa gtaatgttgc tcattttctg aactagaaga
      541 agctcgtaag atttttgtag cactggcaat atgcaatcct aacgttcctt caatgtaatt
      601 gtaaatcgat ttttgtaaaa tatcttcagt aatacctgga ataacatcgg tactcatata
      661 agtttgttcc agaacataag gtttaccgtc aataatacgc aggcggtaaa tattataaac
      721 tggactctgc aaagcgacct gtagtttttc tgctaaaaat tcatttgcaa attctaattt
      781 gaagtgaata atcctcgagt caattgtaga atgagcatct tcagatattt ttgtcagtcc
      841 ccggatatct ctttctggaa cgattaattt tctttttgag ctgccacgag agagaacaaa
      901 ggttccctgc ccacgttttc taaaaattaa gccctcagaa actaacaagt ctaaagcttt
      961 tttgatcgtc attcgactac aggcaaactg tttagctaat gtcatttcat caggcaattg
     1021 ttcgttcatg gtatatttat tacaaagaat cttttcacga atctcattgt aaataaataa
     1081 atattttgta ctcatttaat ctggttctct ctataatatt ctaatagtag tataacaaag
     1141 ttcaagaaat gaggcaaaca caagatatgt atcaacaatt tttaaaaatg tctagacaaa
     1201 ttttaaaatt atgctatact ttatgtatag aataatagaa aggaggttga gcatgaaaaa
     1261 aaagttagta tttcctaatc tgttttggtg gggagcagct tctagcggac ctcagacaga
     1321 aggtcaatat ggaaaagtac atgaaaatgt gatggactac tggttcaaaa cgcatccaga
     1381 agattttttc gataatgtcg gacctcttgt agccagtaac ttttttcata cttacaccga
     1441 agatttccac ttgatgaagg aaattggagt taattctttc cgcacttcca tccaatggag
     1501 tcgactcatc aagaatttag agacaggtga gcctgatcca aaaggtattg ctttctacaa
     1561 tgccatcatt gaagaagcta aaaagaacca gatggatctt gtgatgaatt tacatcattt
     1621 tgatttacca gtggaacttc ttcaaaaata cggtggttgg gaaagcaaac atgtagtgga
     1681 gttattcgtg aagtttgcca agactgcttt cacatgcttt ggagataagg ttcattactg
     1741 gacaactttc aatgagccaa tggtcattcc agaagcagga tacttatatg ctttccatta
     1801 tccaaatcta aaaggaaagg gaaaagaggc cgtacaagtc atctataatc taaaccttgc
     1861 tagtgcaaaa gtgattcaac tatatcgctc attagaactt gatggaaaga ttgggattat
     1921 tttaaacttg acacctgctt atccaagaag taattctcca gaagacttag aagcaagtcg
     1981 atttacagat gacttcttta acaaagtctt cttgaatcca gctgttaaag gaactttccc
     2041 agaaagattg gtaaaacagc tagagagaga tggcgtgtta tggagtcata ccgaaaaaga
     2101 gcttcaactg atgaaatcaa atacggttga ttttcttgga gtaaactact accatccaaa
     2161 acgtgttcaa gcacaagcaa atcctgagga atatcagacg ccctggatgc cagaccaata
     2221 cttcaaagag tatgaatggc cggagcgtcg catgaatcca tatcgtggtt gggaaatttt
     2281 tccgaaagcc atttatgata ttgctatgat tgtgaaggaa gaatatggta atatcccatg
     2341 gtttatcagt gaaaacggaa tgggtgttga aaacgaagca cggtttatcg atgaaaatgg
     2401 agttatcgat gacgtgtatc gtattgaatt ttatgaagaa catttaagat ggctacataa
     2461 agccattgaa gagggaagtc actgttttgg ataccacgct tggaccgcat ttgattgctg
     2521 gtcttggaat aatgcatata agaatcgtta cggatttatc tccgttgatt tagaaacgca
     2581 aaagagaacc atcaagagct caggaagatg gtatcgcaaa gtaagtgaca ataacggttt
     2641 tgaagtagaa attgaggagt aaagagagga gatattcata tgaaaaactc aaaatttata
     2701 gaccaatttg ccacctttgc tggtaaacta gggaaccaaa ttcatttaaa aaccctaaga
     2761 gatgcattcg taacagtaat gccattatat attttggcag gtttaatcgt tcttttgaac
     2821 aacacggtat ttaagtggat tttccaaggg gatacattaa caagattcca atattgggga
     2881 ataacaattg caaacggtac tttaagtatt tcaggtatga ttattgctgt aatggttggt
     2941 tatttcttag ctaaaaacag agatttcgaa aacccgttag cagcatcaat gctatcatta
     3001 gtttctttaa ttgtgatgat gccaaataca gtttctgtag ttcctgacgg agcaaaagat
     3061 gctgtaaaca tttcaggtgt tctttcattc aacaacacag gtacaggcgc aatgttcgcc
     3121 ggggttatcg tagcgattat tgcaacagaa ttattcattg aattatcaaa tgttaaagct
     3181 ttacaaatga atcttggtga aaatattcca ccagctgtta gtagatcatt tagcgtatta
     3241 cttccagtca tgaccgtcat ctccttattt ggggttgttt cagcattatt attcaatata
     3301 actggaatga acttaatctc aatcattaca atctttattc aagaaccaat tcgtcatatt
     3361 ggtacaagct taatcggggt cattattatt tactctttag gaaatatgtt atggctattt
     3421 ggtattcacc aagcagttat ttacagtgcc atcctagaac cattactatt aattaacatt
     3481 actgaaaaca tcactgcagc aaataatgga caagccattc cacacatcat caacctatca
     3541 caaatacaaa cattcgcttt aatgggtggt agtggatcta cattatgttt attaatagca
     3601 acattcttag tgagtcgcaa tgctgtctct aaaaacgtgg ctaaattatc ttttggacct
     3661 ggtatcttca atatcaatga accagtatta ttcggttacc caatcgttta taacatttca
     3721 ttagctattc catttatcac agttccagtc cttggtattt taatcagcta cttagcaaca
     3781 gttacaggat tcatgagtcc tgcatttata caagttcctt ggactacacc agtattctta
     3841 aatgcatggt tagcaacagc aggggacgtg agagcagttc tagttcaatt catcatcttt
     3901 gcacttggag ttcttctata cattccattt atcaaagtta atgacaaagt tgttgaacaa
     3961 gaaatggaag gttaagatta tgaaacaaat tttattagta tgtaatgcgg gaatgtcgac
     4021 aagtatgctt gttaaaaaaa tgcaacaaag tgcgactgaa cgtgggattg aaatctctat
     4081 ccaagcaaaa tcaatgacag aagctaaaaa gaatatccat gaagcagatg tcattctaat
     4141 tgggccacaa attcgttatg agttacttgc tgtgaaagaa attgcaggaa atatccctgt
     4201 agataccatt gatatgcggg attacggaat gatgaatgga gcaaaagttc tggaacaagc
     4261 acttgcatgg ataggagaaa taagatgaca gaagaaatgg aatatatctg ttttcagctc
     4321 attgccaata gtggagcagc caaatcatcc tttattgaag ccatccaact agctaaggca
     4381 ggaaatttaa aagaagcgaa aattaaagtt gaagaagcgg aagactcctt agtagaagcg
     4441 cacaagattc actctaatct catacaaaaa gaagctactg gagaaaaaat aggattctcc
     4501 ttgctattta tgcatgcaga agaccaaatg gcttcaacag agattattca gttgctatct
     4561 aaagaattca tcgatctcta tcaaaataaa taggaacact gtcattccca tctgtcttta
     4621 ttaatgacga ctacattgta ggaacctcca aaagttggac aaaatgattg aaaggattgg
     4681 atcctgcatt acacaagatc caatcctttt cttttactct agtttttata atatgttgaa
     4741 tc
//
LOCUS       DAVLVZ010000095         4420 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000095 DAVLVZ010000000
VERSION     DAVLVZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.095"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(176..1591)
                     /locus_tag="VYN77_001907"
     CDS             complement(176..1591)
                     /locus_tag="VYN77_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1559315.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLLGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLEPS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGTFNRY
                     KKMSFTWYKEVIESNGESLFK"
     gene            complement(1604..3442)
                     /locus_tag="VYN77_001908"
     CDS             complement(1604..3442)
                     /locus_tag="VYN77_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1559316.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVNQGYKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFFAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEVGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3460..4299)
                     /locus_tag="VYN77_001909"
     CDS             complement(3460..4299)
                     /locus_tag="VYN77_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1559317.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
BASE COUNT         1481 a          866 c          576 g         1497 t
ORIGIN      
        1 ataaccaatc tatcctaagt taactattta taatcggctc tctgtaacaa aattaaatta
       61 attaatcccc ctataaaata aagaggttta gaatttgatg tcttccaaac ttctttattc
      121 catatttaat gaaatgccac cttaaccgtg ataatagcta gtcatcaata aaaaactatt
      181 tgaataagga ttctccattt gattcaatca cttctttata ccaagtaaaa gacattttct
      241 tatatcgatt gaatgtacca cttccatcat cgtttcgatc aacataaatg agaccgtacc
      301 ttttagaaag ttgtgcagtg gacatagaaa cacagtcaat acatccccaa gacgtatagc
      361 ccataatttc aacaccatcc tgtagagctt cagcaacttg caataaatgt tctttcatat
      421 actgaattct ataatcatct tggacggtta agttattaag ttcatctttt attagttgat
      481 ctttagcacc taatccattt tctactataa ataatgggat ttgataacgg tcataatatc
      541 tatttaaaat tatacgtagt ccaattggat caatttgcca tccccactca gaaggctcta
      601 aataaggatt tactaagcca ccaataatat tcccttctcc tgaattatac tgtgttggaa
      661 gagcagattg agtcacactc atgtaatagc taaaggataa aaaatctaca gtataatttt
      721 ttaataactc tgcatcttca gctgcaaact ctatgttaat gtcatgttcc ttaaaatatc
      781 tttttgcata attcggataa taacctctaa catgcacatc tgaaaataga taatttagat
      841 tctcatactc atgagtcgcc catacatctt ttggatttgg agtcattgga taagctggca
      901 tagctaatac catacacccc accttaaact ctgaattaat ctcacgagca atttttgtaa
      961 ccaaactcga ggcgactaat tcatgatgta tagcttgata taattcttgt ttcgaaagat
     1021 tctccttagg tatatctatt cctccactag taaacggtaa ttccaaaaca gagtttactt
     1081 cgttaaatgt aagccaatat ttaactttat ctttatacct ttctaaaact gttcgagcaa
     1141 atttttcata aaaatgaatc attctcctat caacccatcc atgatatttt cttgctaaat
     1201 ataatggagt ctcatagtgt gaaagagtta caagtggctc tatcccgtga gcatgtagtt
     1261 catcaaacaa ttcatcataa tatttcaaac cagcttcgtt aggttcttct tcatctcctt
     1321 ttggaaaaat tctactccat gcaatagaag tacgaaaaac attaaagccc atttcagaaa
     1381 acaaggatat atcttcctta tatttatgat aaaaatcaat acctaacaat tttaagttat
     1441 cttctgtagg attttctgtt gcttctccta atccaccttt gggtaacaca tcctgaactg
     1501 ataatccctt accatcttca ttatatgctc cctctacttg attagctgca acagctccac
     1561 cccaaagaaa atcatctgga aaaatggtca taactttcct ccattataat attaccagta
     1621 attccttaga atgctcgatt gtctgattat taggtaatac taatacatct agaaaatcat
     1681 tggtattcgt tacaattact ggtgtaactg tttcgtagcc tttagtcttg attaaattca
     1741 agtccatttc aaaaatcaac tgatttttga aaactctgtc tccttcttct acatgactaa
     1801 taaaaccttg accttttagc tcaacagtat ctaatccaat atgaattagt aactcaacac
     1861 cctcatcact cttcaatcca attgcgtgct tagtcggaaa aatatttgta attttcccgt
     1921 caaatggtgc ataaacctta ccttcacttg ggataatcgc tactccgtct ccaattaatt
     1981 tatctgaaaa tgttttatcc tggacatcgc ttaacggaat gatttctcct gatataggag
     2041 aaaatatcat ttttttattt gaaactccaa cttcaacttc taaattgcta gaactctctt
     2101 cttcatcgat tccaaatata taagctaata caaaggtaat aacaaccgaa atgaccgcca
     2161 caattaaagc atttacaata tttgatggca catcagaata aataaattga ggcaacgcta
     2221 tcaaagatgg gacagcaaat agatatgctt taacactagt aagacctgca aataatcccg
     2281 ctaatccacc accaatcata gctgcataaa gcggtttttt atattttaaa gtcacaccat
     2341 ataatgcagg ttcggtaatt cctgcaagta aggctgagaa acctgctgca aaagcaattt
     2401 gttttgtatt attattttta ctctttaatg caacagccat cgaagcagcc ccttgagcta
     2461 agtttgaccc taacattgct ggaagaatta atacgtctgg agtagcaata gatgccgcaa
     2521 aaaaaatagg tgcaaaagcc caatgcattc cagtcataac aataaatggc ataatagcac
     2581 caagaatagc taatgtaagc catccagcta caccatacat ttgcccaact agatttgata
     2641 atccttcacc aacaattact ccaataggtc cgactacaac taaggcaata cagcttgata
     2701 ctaataatac tagcgtaggt tgcaaaaaac tcttagtaac agctggtgtt aatttagcaa
     2761 ttattttttc aatatatttc atcaaccaaa ccataataag aattggaacg actgatgaac
     2821 cataactagc tggtgtcaca ggtgcaccaa ataaactaag aggattccct gattgcacca
     2881 tttgaacaaa atttggatgg agaagtacac ctgctacaga catagctaat gtagatgtta
     2941 cttttaattt ttgtgatgca gaataagcta ataacagcgg taagaaataa tatggagcat
     3001 ccccaaaaaa tgtcaaaaaa gcaatagtct gagaatctga ttgcaatata ccaagcattg
     3061 gtaaaatgat taccaagact ttcaacatac ctccccctaa cattgctgga atgattggag
     3121 tcatggaacc agcgatatac tcaatgattc tttctaaaat attccctttg tacccttgat
     3181 taactgaatc ggattcaaaa ttgccaagtt taacgaattc tttataataa ttagctacat
     3241 cattaccaag tataatttga tattgtccat tctttttcat aatacctatt acacctggta
     3301 tcttcttcac atcatcatca ttgactaaat tttcatcttt taattctaat cttaaacgtg
     3361 ttacacaatg ggtaactcta ttgacatttt tttcgcctcc aattacatcg aggatttttt
     3421 gtaccgtatc tttataactc atggtattct cctattctat taatctaaat tttttgttaa
     3481 gcgacgaata tgagccatca aataaactaa ttcactagaa gtcagcaaat aattgtactc
     3541 cgtttgtata aacattgcca cctgttcacc acattcatat tctctaggat atttattttt
     3601 cattaatgct aacaagtctt catcatcatc gtcacaataa gttgtatttt caaccaagcg
     3661 atgggcaaat aattttatat gagttatgaa tctataataa tcaatagaat cttggttaaa
     3721 ctcagtacaa tagaaatctt gaatgatttt ctcaattccc ataattattt cagtgatttt
     3781 atacgactct tgaaaattat tttctaaatt agcattaaca aaatgtagcg ctatgaatgc
     3841 agcttcgtca attggaagtt caatacctaa ctcttttttt attatttgta aagccctttt
     3901 cccaagttca aattcttctt tataatattt cgatatttcc cagcgtaggg gattcgaaat
     3961 aataatccct tctttataac gttctatgct cgaagaaata tgatccgtta aattaatata
     4021 gataatttca tcaaaattat tacccaatgt tattttacct aggttaatta ttttttcggt
     4081 gatgtacacc aactccaaag gaacgttaat aaacatctct gtaaaacggg tcatattatc
     4141 tctatttttg agaaaaaaac ttttctcaat atcaattgga tttactttat ctccagtttt
     4201 tcttccaaaa gctattcccc ttccaaaaag aagaatatcg atatcttttt tactttgcgc
     4261 aattacggca ttgtggttta gtattcgttt tatgatcata aaatttccct tttaaaataa
     4321 aaggaaccta cttaaaaaca tcaagaaact tcttctgatg tgattaaata ggttcctgct
     4381 cacttgtatt agtgataaca tccttttatt tcaattttta
//
LOCUS       DAVLVZ010000096         4202 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000096 DAVLVZ010000000
VERSION     DAVLVZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.096"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            737..1729
                     /locus_tag="VYN77_001910"
     CDS             737..1729
                     /locus_tag="VYN77_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1559318.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1731..2549
                     /locus_tag="VYN77_001911"
     CDS             1731..2549
                     /locus_tag="VYN77_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1559319.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2551..3336
                     /locus_tag="VYN77_001912"
     CDS             2551..3336
                     /locus_tag="VYN77_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1559320.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3434..3652
                     /locus_tag="VYN77_001913"
     CDS             3434..3652
                     /locus_tag="VYN77_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1559321.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3637..3834
                     /locus_tag="VYN77_001914"
     CDS             3637..3834
                     /locus_tag="VYN77_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1559322.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
BASE COUNT         1170 a          721 c          909 g         1402 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat tgatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gaaatgatga
      121 aagccttaag atgatgaaag ccttaagtgt tattttataa aggttatttc aagtcgttcc
      181 aaggtaacaa gtctagatca gattgaagct gataaaacga tacaaagaaa atattcaagt
      241 gagctaaaaa aatttattgg attttataat gagattattt gtgaggaaaa tagtttccta
      301 catgtacgaa agaggtgatc gagttggttt aggtagtcga tgcgtgagtt gataattctc
      361 agggtatgga cttctttttc atgaatgagg taaaagagca ggtattgttt agagacaatc
      421 attctgagca tattttctgg atagagggag tatccgattt tatgatcaaa gttaataccg
      481 ccctctggtg agaagatgag taggttggta atttaaacta ttaaacagaa tttttgatta
      541 aaagtattat tttatgagag aaatcctaat ttcacaatcc ataggcaaac tcttgcattt
      601 cgttttttat tggactataa taggttggta taaagccttt tgtagtaata aaatgtagaa
      661 ggtgtagaaa gcaaggattt agaatatttg tagttaaaaa cacaatgttg ctattcctta
      721 cgatagggag atagatatgg caatgataga agtggaacat cttcagaaaa attttgtgaa
      781 gactgttaag gaaccgggct tgaagggggc tttgcgctcc tttattcatc ctgaaaagca
      841 gacctttgaa gcggtcaagg atttgacctt tgaggttcca aaagggcaga ttttaggatt
      901 tatcggggca aatggtgctg ggaagtcgac aaccattaaa atgctgacag gaattttgaa
      961 accaacatct ggtttttgtc ggattaacgg caagattccc caggacaatc ggcaagatta
     1021 tgtcaaagat attggcgtag tctttggaca acgcacccag ctatggtggg atttggctct
     1081 gcaagagacc tacactgtct taaaagagat ttatgatgtg ccaaactcgc tctttcataa
     1141 gcgcatggac tttttgaatg aagtcttgga tttgaaggac tttatcaagg atcccgtgcg
     1201 gactctttca ctgggacaac ggatgcgggc ggatattgcg gcctccttgc tccacaatcc
     1261 caaggttctt tttttagatg agccgaccat tggtttggac gtttcggtta aggataatat
     1321 tcgtcgggca attactcaga tcaatcaaga ggaagaaact accattcttt tgaccactca
     1381 cgatttgagt gatattgagc aactttgtga tcggattttc atgattgaca aggggcaaga
     1441 gatttttgat ggaacggtga gccaactcaa ggagaccttt ggtaagatga agactctctc
     1501 ttttgaactg ctaccaggtc aaagtcatct cgtctctcac tatgaaggtc tgtctgatat
     1561 gaccattgat agacaaggaa acagcctcaa cattgaattt gatagttctc gctaccagtc
     1621 agctgacatt atcaagcaaa ccctgtctga ttttgaaatc cgcgatttga agatggtgga
     1681 tacggatatt gaggatatta tccgtcgctt ctaccgaaag gagctctagg atgatcaaat
     1741 tgtggagacg ttataaaccc tttatcaatg caggggttca ggagttgatt acttaccgag
     1801 tcaactttat tctctatcgg attggcgatg tcatgggggc ttttgtggcc ttttatctct
     1861 ggaaggctgt ctttgattct tcgcaagagt ctttgattca gggcttcagt atggcggata
     1921 tcaccctcta catcatcatg agttttgtga ccaatcttct gactagatcc gattcgtcct
     1981 ttatgattgg ggaggaggtc aaggatggct ccattatcat gcgtttgttg cgaccagtgc
     2041 attttgcggc ctcctatctt ttcaccgagc ttggttccaa gtggttgatt tttatcagcg
     2101 ttggccttcc atttttaagt gtcattgtct tgatgaaaat catatcgggt caaggtattg
     2161 tagaggtgct aggattaact gtcctttatc tttttagctt aacgctcgcc tatctgatta
     2221 actttttctt taatatttgc tttggatttt cagcctttgt gtttaaaaat ctttggggtt
     2281 ccaacctact taagacttcc atagtggctt ttatgtcggg gagtttgatt cccttggcat
     2341 tctttccaaa ggttgtttca gatattcttt cctttttgcc tttttcatcc ttgatttata
     2401 ctccagttat gatcattgtt ggaaaatacg atgccagtca gattcttcag gcactccttt
     2461 tgcagttctt ctggctctta gtgatggtgg gattgtctca gttgatttgg aaacgggtcc
     2521 agtcctttat caccattcaa ggaggttagt atgaaaaaat atcaacgaat gcatctgatt
     2581 tttatcagac aatacatcaa acaaatcatg gaatataagg tggattttgt ggttggtgtc
     2641 ttgggagtct ttctggctca aggcttgaat ctcttgtttc tcaatgtcat ctttcaacat
     2701 attccattcc tagaaggctg gacctttcaa gagatagctt tcatttatgg attttccttg
     2761 attcccaagg gaatggacca tctctttttt gacaatctct gggcactagg gcaacgccta
     2821 gtccgaaaag gggagtttga caagtatttg actcgtccca tcaatcctct ctttcacatc
     2881 ctagttgaaa cctttcagat tgatgccttg ggtgaactct tagtcggtgg tattttattg
     2941 ggaacaacag tgaccagcat tgtttggact cttccaaaat tcctgctttt cctagtttgt
     3001 attccttttg cgaccttgat ttatacttct cttaaaatcg caacagccag tatcgccttt
     3061 tggactaagc agtcaggcgc catgatttac atcttctata tgttcaatga ctttgctaag
     3121 tatccgattt ctatttacaa ttctcttctt cgttggttga ttagctttat cgtgcctttc
     3181 gcctttacag cctactatcc agctagctat ttcttacagg aaaaggatgt gttctttaac
     3241 gtaggaggtt tgatgttgat ttctctggtt ttctttgtta tttcccttaa actttgggat
     3301 aagggcttag attcctacga aagtgcgggt tcgtaaaagc taaagtaaga ctaaaatcaa
     3361 gaaaggaact tatgatgttt gtaattgaag aagtcaagga tgaaaatcaa aaaaggcagt
     3421 tgtcgctgag gttttgaagg atttgccaga atggtttgga atcccagaaa gcacacaagc
     3481 ctatatagaa ggaaccacga cactgcaagt ttggaccgcc tatcaggaga gtgatttgac
     3541 tggatttgta aggttatcct attcgagtga agattgtgca gagattgatt gtctcggcgt
     3601 aaaaaagctt atcaaggtag aaaaattggg agccaattgc ttgctacttt agagagtgaa
     3661 gctcgtaaaa aagttggtta tctgcaggtc aaaacagtgg cagaagcttc taataaagat
     3721 tatgatcgaa caaatgactt ttatcgaggt cttggcttta aaaagttaga gattttttct
     3781 cagctatgga atccgcaaaa tccttgtcag attttgatta aaaagcttga ataatattac
     3841 ttgacatcta ttctcagagt gccatactgt aagtgtaatc gccgatttag ctcagttggt
     3901 agagcaaggc actcgtaaag cctaggttat aggtagataa acgactgagg agttgaaaaa
     3961 atagataggt agaagataac cgttaagcct tattcttagc ggttatttat attatttaat
     4021 agcgctgata tttcatcaat tatgcctgtt ttcgtgtttc tggtagttgt tcaagtttat
     4081 tgctactatt tttgatggta tgaatgtgct tataatgtat cccggttaac gaaagttttg
     4141 gacttatact cttcgaaaat ctcttcaaac cacgtcaacg tcgccttgcc gtgcgtatgg
     4201 tt
//
LOCUS       DAVLVZ010000097         3907 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000097 DAVLVZ010000000
VERSION     DAVLVZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.097"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            344..571
                     /locus_tag="VYN77_001915"
     CDS             344..571
                     /locus_tag="VYN77_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559323.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            859..3756
                     /locus_tag="VYN77_001916"
     CDS             859..3756
                     /locus_tag="VYN77_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV1559324.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAVLTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDKVVISNLRDNPEAKD
                     PAAPSPLNIDMVLVQDPTTKRIFSIYDMFPEGRAVFGMPKTPEKAYEKIGDKTYQILY
                     KQGESGHYTVRENGEVYNAQNQKTDYRVVVNPTEPGYRDKGNLYKGQELIGNIYFAHS
                     TKNPFRVANTSYLWMSYSDDDGKTWSAPRDITPGLRKDWMKFLGTGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NERAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1340 a          623 c          872 g         1072 t
ORIGIN      
        1 gtgttttgag catcatgtgg ctagcttctt agtttgctct ttgattttca ttgagtataa
       61 aaacagatga gtttctgttt tctttttatg gactataaat gttcagctga aactactttc
      121 aaggacatta ttatataaaa gaattttttg aaactaaaat ctactatatt acactatatt
      181 gaaagcgttt taaaaataag gtataataaa tttattaaca cttataaaaa gtgatagaat
      241 ctatctttat gtatatttaa agatagattg ctgtaaaaat agtagtagct atgcgaaata
      301 acagatagag agaagggatt gaagcttaga aaaggggaat aatatgatat ttaaggcatt
      361 caagacaaaa aagcagagaa aaagacaagt tgaactactt ttgacagttt ttttcgacag
      421 ttttctgatt gatttatttc ttcacttatt tgggattgtc ccctttaagc tggataagat
      481 tctgattgtg agcttgatta tatttcccat tatttctaca agtatttatg cttatgaaaa
      541 gctatttgaa aaagtgttcg ataaggattg agcaggaagt atggtgtaaa tagcataagc
      601 tgatgtccat catttgctta taaagagata gtttagttta attgcagcgg tgtcctggta
      661 gataaactag attggcagga gtctgattgg agaaaggaga ggggaaattt ggcaccaatt
      721 tgagatagtt tgtttagttc atttttgtca tttaaatgaa ctgtagtaaa agaaaattaa
      781 taaaagacaa actaagtgca ttttctggaa taaatgtctt atttcagaaa tcgggatata
      841 gatatagaga ggatcagtat gaatcggagt gttcaagaac gtaagtgtcg ttatagcatt
      901 aggaaactat cggtaggagc ggtttctatg attgtaggag cagtggtatt tggaacgtct
      961 cctgttttag ctcaagaagg ggcaagtgag caacctctgg caaatgaaac tcaactttcg
     1021 ggggagagct caaccctaac tgatacagaa aagagccagc cttcttcaga gactgaactt
     1081 tctggcaata agcaagaaca agaaaggaaa gataagcaag aagaaaaaat tccaagagat
     1141 tactatgcac gagatttgga aaatgtcgaa acagtgatag aaaaagaaga tgttgaaacc
     1201 aatgcttcaa atggtcagag agttgattta tcaagtgaac tagataaact aaagaaactt
     1261 gaaaacgcaa cagttcacat ggagtttaag ccagatgcca aggctccagc attctataat
     1321 ctcttttctg tgtcaagtgc tactaaaaaa gatgagtact tcactatggc agtttacaat
     1381 aatactgcta ctctagaggg gcgtggttcg gatgggaaac agttttacaa taattacaac
     1441 gatgcaccct taaaagttaa accaggtcag tggaattctg tgactttcac agttgaaaaa
     1501 ccgacagcag aattgccaaa tggtcgagtt cgcctctacg taaacggggt attatctcga
     1561 acaagtctga aatctggcaa tttcattaaa gatatgccag atgtaacgca tgtgcaaatc
     1621 ggagcaacca agcgtgccaa caatacggtt tgggggtcaa atctacagat tcggaatctc
     1681 actgtgtata atcgtgcttt aacaccagaa gaggtacaaa aacgtagtca actttttaaa
     1741 cgctcagatt tagaaaaaaa actacctgaa ggagcggttt taacagagaa aacggacata
     1801 ttcgaaagcg ggcgtaacgg taaaccaaat aaagatggaa tcaagagtta tcgtattcca
     1861 gcacttctca agacagataa aggaactttg atcgcaggtg cagatgaacg ccgtctccat
     1921 tcgagtgact ggggtgatat cggtatggtc atcagacgta gtgaagataa tggaaagaca
     1981 tggggagata aggtggttat ctccaatctt cgagataatc ctgaagctaa agatcctgct
     2041 gcgccatcgc ctctaaatat tgatatggtt ttggttcaag acccgacaac aaagagaatc
     2101 ttctcaattt atgatatgtt cccagaaggt cgagcagttt ttggaatgcc aaaaacacct
     2161 gaaaaagctt atgaaaagat aggggataaa acttatcaaa tcttgtataa acaaggagag
     2221 tctggtcatt atactgttcg tgagaatgga gaagtgtata atgcacaaaa tcaaaagacg
     2281 gattatcgtg ttgtagtgaa tccaacagaa cctggctata gagataaagg aaatctttac
     2341 aaaggtcagg aattgattgg aaatatctat tttgcacaca gtacaaaaaa tccatttaga
     2401 gtagccaata cgagctatct atggatgtca tatagtgacg atgatggtaa aacttggtct
     2461 gcaccgagag acattactcc aggtcttcgc aaggattgga tgaagttcct aggaacaggt
     2521 cctggaacag gaattgtact tcggaatggg cctcacaagg gacggatttt gataccggtt
     2581 tatacgacta ataatgtatc tcacttaaat ggctcgcaat cttctcgtgt catctattca
     2641 gatgatcatg gaaaaacttg gcatgctgga gaagcggtca acgataaccg tcaggtagac
     2701 ggtcaaaaga tccactcttc tacgatgaac aatgaacgtg cgcaaaatac agaatcaacg
     2761 gtggtacaac taaacaatgg agatgttaaa ctctttatgc gtggtttgac tggagatctt
     2821 caggttgcta caagtaaaga cggaggagtg acttgggaga aggatatcaa acgttatcca
     2881 caggttaaag atgtctatgt tcaaatgtct gctatccata cgatgcacga aggaaaagaa
     2941 tacatcatcc tcagtaatgc aggtggaccg aaacgtgaaa atgggatggt ccacttggca
     3001 cgtgtcgaag aaaatggtga gttgacttgg ctcaaacaca atccaattca aaaaggagag
     3061 tttgcctata attcgctcca agaattagga aatggggagt atggtatctt gtatgaacat
     3121 actgaaaaag gacaaaatgc ctatacccta tcatttagaa aatttaattg ggactttttg
     3181 agcaaagatc tgatttctcc taccgaagcg aaagtgaagc gaactagaga gatgggcaaa
     3241 ggagagatgg gcaaaggagt tattggcttg gagttcgact cagaagtatt ggtcaacaag
     3301 gctccaaccc ttcaattggc aaatggtaaa acagcgactt tcctaaccca gtatgatagc
     3361 aagaccttgt tgtttgcagt agataaggaa gatatcggac aggaaattat tggtatagct
     3421 aaaggaagca tcgaaagtat gcataatctt cctgtaaatc tagcaggtgc cagagttcct
     3481 ggcggagtaa atggtagcaa agcagcggtg catgaagttc cagaatttac agggggagtt
     3541 aatggtacag agccagctgt tcatgaaatc gcagagtata agggatctga ttcgcttgta
     3601 actcttacta caaaagaaga ttatacttac aaagctcctc ttgctcagca ggcacttcct
     3661 gaaacaggaa acaaggagag tgacctccta gcttcactag gactaacagc tttcttcctt
     3721 ggtctgttta cgctagggaa aaagagagaa caataagaga agaattctaa acatttgatt
     3781 ttgtaaaaat agaaggagat agcaggtttt caagcctgct atcttttttt gatgacattc
     3841 aggctgatac gaaatcataa gaggtctgaa actactttca gagtagtctg ttctataaaa
     3901 tatagta
//
LOCUS       DAVLVZ010000098         3762 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000098 DAVLVZ010000000
VERSION     DAVLVZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.098"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            64..282
                     /gene="nrdH"
                     /locus_tag="VYN77_001917"
     CDS             64..282
                     /gene="nrdH"
                     /locus_tag="VYN77_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1559325.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            363..2522
                     /gene="nrdE"
                     /locus_tag="VYN77_001918"
     CDS             363..2522
                     /gene="nrdE"
                     /locus_tag="VYN77_001918"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1559326.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIEIAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            2710..3672
                     /gene="nrdF"
                     /locus_tag="VYN77_001919"
     CDS             2710..3672
                     /gene="nrdF"
                     /locus_tag="VYN77_001919"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1559327.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
BASE COUNT         1163 a          810 c          704 g         1085 t
ORIGIN      
        1 attgacaaaa ttttattttc tgatatacta agatagtatt aaattttgaa gaggagttac
       61 acaatggtaa ccgtttattc taaaaacaat tgtgtccaat gtaaaatgac caagcgtttc
      121 ttggacagta ataatgtctc ttatcgtgaa atcaatcttg atgagcaacc tgagtacgtc
      181 gatcaagtta aagagctcgg ttttagcgca gctcctgtta tccaaacacc aactgaagtc
      241 ttttcaggtt tccaaccagg aaaactgaaa caattagcat aatcttagta catcatccag
      301 aagaaactgc ttttagggct aacttagagg cctttctttt gtaattagat aagggaaatt
      361 ttatgggatt aaaacatctt gaagacgtga cttacttccg tcttaataac gaaattaacc
      421 gtcctgttaa tggacaaatc atgcttcata aagacaagga agccttggat gctttcttta
      481 aagaaaatgt agttccaaac actatggttt ttgattcaat caaagacaaa atcaattacc
      541 tcattgaaca caactacatc gaaatagcct ttatcaagaa ataccgtcca gagttcttgg
      601 aagaattggc tcaatttatc aaagaccaaa acttccaatt caagtcattc atggcagctt
      661 ataaatttta caatcaatat gccttaaaga ctaatgacgg tgaatactat cttgaaaata
      721 tggaagaccg tgtcttcttc aacgcccttt atttcgctga tgggaatgaa gctgttgcaa
      781 tcgatattgc caatgaaatc atccaccaac gctaccaacc tgctactcct tccttcttga
      841 atgctggacg tgctcgtcgt ggggagttgg tatcttgttt cctaatccaa gtgacggatg
      901 atatgaactc tatcggacgt tctatcaact cagctcttca actttcacgt attggtggtg
      961 gtgtgggaat taccctcagc aaccttcgtg aagctggtgc acctatcaaa ggctatgaag
     1021 gagctgcttc aggtgtcgtt cctgttatga agctttttga agacagcttc tcttactcca
     1081 accaattggg gcaacgtcaa ggtgctggtg ttgtctacct caacgtcttt cacccagata
     1141 tcatcgcttt cctttcaact aagaaagaaa acgctgatga aaaagtacgt gtcaagactc
     1201 tatcacttgg tgttgtagta cctgataaat tctacgaatt ggctcgtaaa aatgaagaaa
     1261 tgtacctctt cagcccttac tctgtagaaa aagaatacgg tgtgccattc aattacatcg
     1321 acattactga aaaatacgat gaattggtcg caaatccaaa tattcgcaag acaaaaatca
     1381 aggcgcggga tttggaaact gaaatctcta aattgcaaca agagtctggc tatccttatg
     1441 tagtcaacat cgatacggct aaccgtgcaa atcctgttga tggaaagatt atcatgagta
     1501 acttgtgttc tgagattctt caagttcaag aaccaagctt gatcaacgat gctcaagaat
     1561 tccttcaaat gggaacagat gtttcatgta acctgggttc aaccaacgtg gttaacatga
     1621 tgacttcacc tgattttggt cgttctatcc gtgctatggt tcgtgccctt actttcgtta
     1681 cagatagttc acatatcgta gctgtaccaa cgattgacca tggaaatagc caagcccaca
     1741 cctttggtct cggtgccatg ggactgcaca gctaccttgc ccaacaactc attgaatatg
     1801 gttcacctga gtctgttgaa tttacaagca tctactttat gcttatgaac tactggacct
     1861 tggtagagtc aaacaatatt gcacgtgaac gtggtattac cttccacaac tttgaaaaat
     1921 cagactatgc taacggaagt tactttgaca agtatgtaac tggtgaattt gttccaacat
     1981 cagaccgtgt taaagaactc ttcaaaaatg tctttatccc tggtgttgct gattgggctg
     2041 aacttcgcga caaggttcaa gaagatggtc tttaccacca aaatcgcctt gctgtagcgc
     2101 caaatggttc tatcagctat atcaacgacg tttctgcttc tatccacccg attacgcaac
     2161 gtatcgaaga acgccaagag aagaaaattg gtaaaatcta ctaccctgct gctggcttgt
     2221 ctacagaaac cattccttac tacacttctg cctacgatat ggatatgcgt aaagtcatcg
     2281 atgtttacgc tgctgcgact gaacacgtgg atcaaggact ttcactcacc ctcttcatgc
     2341 gcagtgacat tccaaaaggc ctttacgaat ggaagaggga aaataaacaa acgacacgtg
     2401 atttgtccat ccttcgtaac tatgccttta acaagggaat caagtctatc tactacgtcc
     2461 gtacctttac agatgacggt ggagaagtcg gcgccaacca atgtgaaagc tgtgtgattt
     2521 agtttctagc atagaaacat tatttttccc taagcagcac tgataaaatc aaatctacta
     2581 tgtcataaca taatttatgt aacattttaa aagtcgggct tccgactttt tgtgcgatac
     2641 tcctatagaa ttatggtaaa gtagaaataa ttgtgagttc gcaatactaa acacagaaag
     2701 agattttaaa tggaaactta ctacaaagcc attaactgga atgccatcga agatgtcatc
     2761 gacaaatcaa cttgggaaaa actgacggag caattttggc tcgatacacg tattccctta
     2821 tcaaacgact tggatgactg gagaaagcta tctaacaaag agaaagactt ggtcggaaaa
     2881 gtttttggtg gtttaaccct tcttgacact atgcaatctg aaactggggt tcaagccctt
     2941 cgcgcagaca tccgtacacc acatgaggaa gctgtcttca ataacatcca atttatggaa
     3001 tctgtccacg ctaaatctta ctcatcaatc ttttctacct tgaatactaa ggctgagatt
     3061 gaagaaattt tcgaatggac caataccaat ccttacctac aaaagaaggc tgagattgtc
     3121 aacgaaatct acctaaacgg cagcccactt gaaaagaaag ttgccagcgt cttcctcgaa
     3181 accttcctct tctactctgg tttcttcact cccctctact atctcggtaa caacaaactt
     3241 gccaacgttg cagaaatcat taaattgatt attcgtgacg agtctgttca cggaacctac
     3301 attggttaca aattccaact tggtttcaat gaattacctg aagaagagca agaaaaactc
     3361 aaagaatgga tgtacgacct actctatact ctttatgaaa atgaagaagg ctacacagag
     3421 agtctttatg acggtgttgg ttggactgaa gaggttaaaa ccttccttcg ctacaatgcc
     3481 aataaagctc tcatgaacat gggacaagat ccacttttcc cagattcagc tgaagatgtc
     3541 aacccaatcg ttatgaacgg tatttcaaca ggaacttcca accatgactt cttctctcaa
     3601 gtcggaaatg gttaccttct tggcgaagtt gaagccatgc aagatgatga ctacaactac
     3661 ggtttggact aatactcttc gaaaatcaaa ttcaaaccac gtcagcttcg ccttgccgta
     3721 ctcaagtaca gcctgcggct agcttcctag tttgctcttt ga
//
LOCUS       DAVLVZ010000099         3753 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000099 DAVLVZ010000000
VERSION     DAVLVZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.099"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..801
                     /locus_tag="VYN77_001920"
     CDS             <1..801
                     /locus_tag="VYN77_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1559328.1"
                     /translation="STNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDF
                     WTYSMGDAFICVSETMKKRLESIIKNKKIVAINNGINVSNSDLDVVGVQDFKKEFCIP
                     NNKKIICYVGRLDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICHLKC
                     RDNFRLLGEIYPATIVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLASNVGGLSEII
                     EHRVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHESI"
     gene            825..1700
                     /locus_tag="VYN77_001921"
     CDS             825..1700
                     /locus_tag="VYN77_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1559329.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGSKNSRGKYISFLDDDDE
                     YMSDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNSLFVQMVHNIA
                     GTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGCQIDIVREFLVNYYAHSKENG
                     ITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKV
                     IKKAREEKIFNEFLF"
     gene            1787..3403
                     /locus_tag="VYN77_001922"
     CDS             1787..3403
                     /locus_tag="VYN77_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559330.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
BASE COUNT         1366 a          399 c          708 g         1280 t
ORIGIN      
        1 tcaactaatt attttttata tatgttaaaa aaacttgaca agaatggatt tggaaaggtt
       61 aagacagtga taacatgcca tggatgggtt gaatataact taaaaaagaa attcttgact
      121 tattttgatt tttggacata ttctatggga gatgctttta tttgtgtatc tgaaactatg
      181 aaaaaaagat tggagagtat aataaaaaat aagaagatag ttgcaataaa taacggaatt
      241 aatgtatcaa atagcgattt agatgtagta ggtgttcaag actttaaaaa agaattttgt
      301 attccaaata acaaaaaaat catttgttat gttggaaggt tggatccaga aaaaaggccg
      361 gatagattcc ttgaatttgc agaaaaatta tttttagtca gagaagatgt gatttttatt
      421 atggctggaa atggtagtat gtgggctgct ttgaaagaaa aaatttgcca tttgaaatgt
      481 agagataatt tcaggttact tggtgaaatc tatccagcta ctatagttta ccaaatatcg
      541 gatttattgt atattccttc tgatacagaa ggaataccta tgtgtgtttt agagtcaatg
      601 tcacagggaa ctccggtttt agctagtaat gttggcgggt taagtgaaat tattgaacat
      661 agggttgatg gatttttatt tgagaaggaa gatgttgagg gagtgtgtgc ttgtgctaat
      721 tttttactca atgattctga gtatttgaaa tatataggtg agaatagtaa atcaaaaata
      781 agaaaacatg agagtatatg atgaattatt agagaagagt agtcatggat aggaatatag
      841 atcaagaatt agtaagtatt ataataccaa cacataatag gtatgaatct ctgattagag
      901 ctgtgaaaag ttgtttacat caaagttata aaaatatcga agttataatt attgatgata
      961 attatagtaa tgtaaattta agaaataaaa ttatccatca atttggctat accaatcata
     1021 gaattaagtt aattttaagt aatgaagatt taggtgcaac taatgccaga aacataggta
     1081 gcaaaaattc tagaggtaag tatatatcat ttttagacga tgatgatgaa tatatgtcag
     1141 atcgaatttt aaagttgatg gcttgtttta aaaagagtag aatgaagaat ttagctttag
     1201 tttatagtta tggaataata atttatccaa atggtacacg agaagaggag aagaccgatt
     1261 ttgttggaaa ttccttgttt gttcaaatgg ttcacaatat agcaggtacg tcattttggt
     1321 tgtgtaaaaa agaggtgcta gaattaatta atggttttga gaaaatagat tcacatcagg
     1381 acggtgttgt tttattaaaa ctacttgctc aaggatgcca aattgatata gtgcgagaat
     1441 tcttggtgaa ttactacgct cacagtaaag aaaacggtat cactggagtg acacaaaaaa
     1501 caattaatgc agatgaagaa tattataatt actgtaggaa atattttaat ttattgagtt
     1561 tcaatgagag aatattggtt acaaagaaat attattcttt aaacataaag cggttactat
     1621 taataggaga caaatgcaag gctttaaaag taatcaagaa ggcaagagaa gaaaaaattt
     1681 ttaacgaatt tcttttttga aatatatgtt attatatcat tttggttttt tctattgtat
     1741 atatgacaac tatgttcaat taaaatttag aaagtgagaa actattgtgt atactattat
     1801 aaaatcaaat ataaaaaaat ttagtttatt aacgatattt attgttgctg gtcaattatt
     1861 gctaatttat gcagcaacta ttaatgctct ggtgttgaat gaattaattg cgatgaattt
     1921 agagcggttt ttgaaattgt caatctacca aatgattgtc tggtgtggga taatattcct
     1981 tgactgggta gtgaaaaatt atcaggttga agtgatccaa gagtttaatc tagagattcg
     2041 aaatagagtt gccacagaca tctctaactc tacctatcaa gaatttcata gtaaatcatc
     2101 aggaacatat ctttcgtggc taaataatga tgttcagact ttaaatgatc aggcttttaa
     2161 acaacttttt ttagtaataa aaggaatttc tggtactata tttgcagttg tgactcttaa
     2221 tcactatcat tggtcattga ctgtagccac cttgttttca ttaatgatta tgctacttgt
     2281 accaaaaatc tttgcatcga aaatgcgaga agttagtcta aatttaacta accaaaatga
     2341 agctttttta aaatctagtg agactatatt gaatggattt gatgtgttag cgtccttgaa
     2401 tcttttatat gtattgccta agaaaattaa agaagcagga attttattaa agatggttat
     2461 acaaagaaag acaactgtag aaacgttagc aggcgctatt agcttctttc tcaatatttt
     2521 ttttcagata tctctcgttt ttttaacagg ctatcttgca ataaaaggaa tagtgaaaat
     2581 tggtactatt gaagcaatag gagcactaac aggtgttatt tttacagcgc taggtgaatt
     2641 aggaggtcaa ttatcctcta ttattggtac gaagcctatt tttttaaaat tgtattcaat
     2701 taatccaatt gagtcaaata aaatgaatga tatcgaacca aatgaggtga atagagattt
     2761 tccgttatat gaagcaaaaa atatttgcta taagtatgga gataaagaaa tattaaaaaa
     2821 cttaaatttt tgttttcaac gtaatgaaaa gtatttaatt ttaggtgaaa gtggaagcgg
     2881 gaaatctaca ttattaaaat tattgaatgg ctttttgaga gattatagtg gagaattgcg
     2941 attctgcggg gatgatataa aaaaaacctc ctatttaaat atggtttcga atgttctata
     3001 tgtagatcaa aaagcttatt tgtttgaagg tacgattaga gataatattt tattggaaga
     3061 aaattatact gatgaagaaa tactacagtc tttagagcaa gttggtttga gtgtaaaaga
     3121 ttttcctaat aacattttag attattatgt tggtgatgat gggagattac tgtcaggagg
     3181 gcagaaacaa aaaattactt tagctagagg gctaattaga aataagaaaa tagtattaat
     3241 tgacgaggga acttctgcta tcgataggag aacttcgtta gcgattgaac gtaagatatt
     3301 agatagagag gatttgactg tcattattgt tacccatgct ccgcatccgg aacttaaaca
     3361 atattttact aagatatatc aatttccaaa ggattttatt taagaatata ggggagggaa
     3421 acatgagatt ctattgtgaa gcttatgagg taattactga agagaaaaaa gtatcatttt
     3481 tttatgtaga tgagaaaccc ttaaggtatc tgttatttga ctatatattt ctactagttc
     3541 tagctaaaaa aaaatatccc aatcttatga actgcacccc aaaagttaga tttattctgt
     3601 ctaacttttg gggtgcagtt cattttgcat agttttggca ttcatcctag atatccatat
     3661 aaaatgtctg agattgttga aatgtctaaa aaaatatcag atctaaacga taaaaaagaa
     3721 tttcacaaaa agaactgtga gttgaggttt caa
//
LOCUS       DAVLVZ010000100         3723 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000100 DAVLVZ010000000
VERSION     DAVLVZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.100"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            105..584
                     /locus_tag="VYN77_001923"
     CDS             105..584
                     /locus_tag="VYN77_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1559331.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            678..749
                     /locus_tag="VYN77_001924"
     tRNA            678..749
                     /locus_tag="VYN77_001924"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Glu,seq:ttc)
     gene            1002..2547
                     /locus_tag="VYN77_001925"
     rRNA            1002..2547
                     /locus_tag="VYN77_001925"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2599..2671
                     /locus_tag="VYN77_001926"
     tRNA            2599..2671
                     /locus_tag="VYN77_001926"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2632..2634,aa:Ala,seq:tgc)
     gene            2796..>3723
                     /locus_tag="VYN77_001927"
     rRNA            2796..>3723
                     /locus_tag="VYN77_001927"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1123 a          700 c         1051 g          849 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtttataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggaccaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caaaaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aagcaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ttagtaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagtttgaaa aggctgtata atagtaagag
      661 ttgaaaataa caactcaggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg
      721 taacacgggt tcgaatcccg tacggactat ggtatgttgc ggttggaaca cttgatgaaa
      781 aaaagtttaa aaaaacttaa aaatcttcaa aaaagtgttg acaagtgaaa gtggctgtga
      841 tatactaata tagttgtcgc ttgagagaag caagtgacaa agacctttga aaactgaaca
      901 agacgaacca atgtgcaggg cgctacaaca taagttgtag tactgaacaa tgaaaaaaac
      961 aataaatctg tcagtgacag aaatgagtaa gaactcaaac ttttaatgag agtttgatcc
     1021 tggctcagga cgaacgctgg cggcgtgcct aatacatgca agtagaacgc tgaaggagga
     1081 gcttgcttct ctggatgagt tgcgaacggg tgagtaacgc gtaggtaacc tgcctggtag
     1141 cgggggataa ctattggaaa cgatagctaa taccgcataa gagtggatgt tgcatgacat
     1201 ttgcttaaaa ggtgcacttg catcactacc agatggacct gcgttgtatt agctagttgg
     1261 tggggtaacg gctcaccaag gcgacgatac atagccgacc tgagagggtg atcggccaca
     1321 ctgggactga gacacggccc agactcctac gggaggcagc agtagggaat cttcggcaat
     1381 ggacggaagt ctgaccgagc aacgccgcgt gagtgaagaa ggttttcgga tcgtaaagct
     1441 ctgttgtaag agaagaacga gtgtgagagt ggaaagttca cactgtgacg gtatcttacc
     1501 agaaagggac ggctaactac gtgccagcag ccgcggtaat acgtaggtcc cgagcgttgt
     1561 ccggatttat tgggcgtaaa gcgagcgcag gcggttagat aagtctgaag ttaaaggctg
     1621 tggcttaacc atagtaggct ttggaaactg tttaacttga gtgcaagagg ggagagtgga
     1681 attccatgtg tagcggtgaa atgcgtagat atatggagga acaccggtgg cgaaagcggc
     1741 tctctggctt gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag gattagatac
     1801 cctggtagtc cacgctgtaa acgatgagtg ctaggtgtta gaccctttcc ggggtttagt
     1861 gccgtagcta acgcattaag cactccgcct ggggagtacg accgcaaggt tgaaactcaa
     1921 aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga
     1981 agaaccttac caggtcttga aatccctctg accgctctag agatagagtt ttccttcggg
     2041 acagaggtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag
     2101 tcccgcaacg agcgcaaccc ctattgttag ttgccatcat tcagttgggc actctagcga
     2161 gactgccggt aataaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg
     2221 acctgggcta cacacgtgct acaatggctg gtacaacgag tcgcaagccg gtgacggcaa
     2281 gctaatctct taaagccagt ctcagttcgg attgtaggct gcaactcgcc tacatgaagt
     2341 cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg ggccttgtac
     2401 acaccgcccg tcacaccacg agagtttgta acacccgaag tcggtgaggt aaccgtaagg
     2461 agccagccgc ctaaggtggg atagatgatt ggggtgaagt cgtaacaagg tagccgtatc
     2521 ggaaggtgcg gctggatcac ctcctttcta aggataagga actgcgcatt ggtcttgttt
     2581 agtcttgaga ggtcttgtgg ggccttagct cagctgggag agcgcctgct ttgcacgcag
     2641 gaggtcagcg gttcgatccc gctaggctcc attggtgaga gatcaccaag taatgcacat
     2701 tgaaaattga atatctatat caaatagtaa caagaaaata aaccgaaaac gctgtagtat
     2761 taataagagt ttatgactga aaggtcagaa aataaggtta agttaataag ggcgcacggt
     2821 ggatgccttg gcactaggag ccgacgaagg acgtgacaaa cgacgatatg ccttgggtag
     2881 ctgtaagtaa gcgatgatcc agggatttcc gaatggggga acccaacagg taatacctgt
     2941 tacccacatc tgttaaggat gtgaggagga agacgcagtg aactgaaaca tctaagtagc
     3001 tgcaggaaga gaaagcaaaa gcgattgcct tagtagcggc gagcgaaacg gcagaagggc
     3061 aaaccgaaga gtttactctt cggggttgta ggactgcaat gtggactcaa agattataga
     3121 agaatgattt gggaagatca gccaaagaga gtaatagcct cgtatttaaa atagtctttg
     3181 tacctagcag tatcctgagt acggcgggac acgtgaaatc ccgtcggaat ctgggaggac
     3241 catctcccaa ccctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
     3301 gtgaaaagca ccccgggagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
     3361 tcgagcccgt taatgggtga gagcgtgcct tttgtagaat gaaccggcga gttacgttat
     3421 gatgcgaggt taagttgaag agacggagcc gtagggaaac cgagtctgaa tagggcgcct
     3481 tagtatcatg acgtagaccc gaaaccatgt gacctaccca tgagcaggtt gaaggtgcgg
     3541 taagacgcac tggaggaccg aaccagggca cgttgaaaag tgcttggatg acttgtgggt
     3601 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
     3661 tagcgtcgac attagagatt cttggaggta gagcactgtt tgggtgaggg gtccatcccg
     3721 gat
//
LOCUS       DAVLVZ010000101         3605 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000101 DAVLVZ010000000
VERSION     DAVLVZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.101"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            208..471
                     /locus_tag="VYN77_001928"
     CDS             208..471
                     /locus_tag="VYN77_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1559332.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            477..2210
                     /gene="ptsP"
                     /locus_tag="VYN77_001929"
     CDS             477..2210
                     /gene="ptsP"
                     /locus_tag="VYN77_001929"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1559333.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            2465..>3605
                     /locus_tag="VYN77_001930"
     CDS             2465..>3605
                     /locus_tag="VYN77_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1559334.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSANDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRENLSNLRTYRRQNSDNTPRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLIFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPS"
BASE COUNT         1186 a          707 c          775 g          937 t
ORIGIN      
        1 aatgattatc ctataaattg tttttgaaga aactatatcc tgtgttttgt tatctaggat
       61 agaagatttt cagcaagtta tctgaaagga aattgaagga aaatccagaa aatgcttgaa
      121 aaaaatccta gaagatggta taatactaaa ttgtaagggt tatcacatat aactcaaaaa
      181 aagaaagaac aaaaggagag tcaaactatg gcttctaaag atttccacgt agtggcagaa
      241 acaggtattc acgcacgtcc agcaacattg ttggtacaaa ctgctagcaa atttgcttca
      301 gatatcactc ttgagtacaa aggtaaatca gttaacctta aatcaattat gggtgttatg
      361 agtcttggtg ttggtcaagg tgctgacgta actatctcag ctgaaggtgc agatgcagat
      421 gacgctatcg ctgcaatctc agaaacaatg gaaaaagaag gattggcata agggaaatga
      481 cagaaatgct taaaggaatc gcagcatctg acggtgttgc agttgcaaaa gcatatctac
      541 tcgttcagcc ggatttgtca tttgagacta ttacagtcga agatacaaac gcagaagaag
      601 ctcgccttga tgccgctcta caggcatcac aagacgagct ttctgttatt cgcgagaaag
      661 cagtaggtac gctcggtgaa gaagcagctc aagtttttga tgctcactta atggttcttg
      721 ctgacccaga aatgatcagc caaatcaagg aaactatccg tgcgaagaaa gtgaatgcag
      781 aagcaggtct gaaagaagtt acagatatgt ttatcactat ctttgaaggc atggaagaca
      841 acccatacat gcaagaacgc gcagcggata tccgcgacgt gacaaaacgt gtattggcaa
      901 accttcttgg taaaaaattg ccaaacccag cttctatcaa tgaagaagtg attgtgattg
      961 cgcatgactt gactccttca gatacagctc aattggacaa aaactttgta aaagcttttg
     1021 taaccaacat tggtggacgt acaagccact cagctatcat ggcacgtaca cttgaaattg
     1081 ctgctgtatt aggtacaaac aacatcactg aaatcgttaa agacggtgac atccttgctg
     1141 ttaacgggat cactggagaa gtgattatca acccaacaga tgaacaagcg gcagaattta
     1201 aagcagctgg tgaagcctat gcgaaacaaa aagctgaatg ggcacttttg aaagatgctc
     1261 aaacagtgac tgctgacggt aaacacttcg agttggctgc caatattggt actccaaaag
     1321 acgttgaagg tgttaacaac aacggtgcag aagctgttgg actttaccgt acagagttct
     1381 tgtacatgga ttctcaagac ttcccaactg aagatgagca gtatgaagca tacaaggctg
     1441 ttcttgaagg aatgaacggt aaacctgttg tcgttcgtac aatggatatc ggtggagata
     1501 aggaacttcc ttacttcgat atgcctcacg aaatgaaccc attccttgga ttccgtgctc
     1561 ttcgtatctc tatctctgag actggagatg ctatgttccg cacacaaatc cgtgctcttc
     1621 ttcgtgcgtc tgttcacggt caattgcgta tcatgttccc aatggttgcg ctcttgaaag
     1681 aattccgtgc agcgaaagca gtctttgatg aagaaaaagc aaaccttctt gctgaaggtg
     1741 ttgcagttgc ggataacatt caagttggta tcatgatcga gattcctgca gcggctatgc
     1801 ttgcagacca atttgctaaa gaagttgact tcttctcaat tggtacaaat gacttgatcc
     1861 aatatacaat ggcagcagac cgtatgaacg aacaagtttc atacctttac caaccataca
     1921 acccatcaat cctacgcttg attaacaatg tgatcaaagc agctcacgct gaaggtaaat
     1981 gggctggtat gtgtggtgag atggctggtg accaacaagc tgttccactt cttgtcggaa
     2041 tgggcttgga tgagttctct atgtcagcaa catctgtact tcgtacacgc agcttgatga
     2101 agaaactcga cacagctaag atggaagagt acgcaaaccg tgcccttaca gaatgctcaa
     2161 caatggaaga agttcttgaa cttcaaaaag aatacgttaa ttttgattaa tcgaaaagtc
     2221 cctgcaactc agttacaggg actttttttg atattttaaa aagaattttc aagaaaatct
     2281 ttcttataga aagtccaacc ttgaaaaagt agtggtcaga acaaaaaata cttaaatggt
     2341 tcataaaatt cttgacaagt tggagattta ggagtaaact attaaccagt taagtaatag
     2401 agaggagttt ctgcaattta gaaatgaatt gcaactagaa atatcaaata gaaagagagt
     2461 ttcgatgaaa attaataaga aataccttgt tggttctgcg gcagctttga ttttaagtgt
     2521 ttgttcttac gagttgggac tgtatcaagc tagaacggtt aaggaaaata atcgtgtttc
     2581 ctatatagat ggaaaacaag cgacgcaaaa aacggagaat ttgactcctg atgaggttag
     2641 caagcgtgaa ggaatcaatg ctgagcaaat cgtcatcaag ataacagacc aaggctatgt
     2701 cacttcacat ggggaccact atcattatta caatgggaag gttccttatg acgctatcat
     2761 cagtgaagaa ttgctgatga aggatccaaa ctaccagcta aaggacgagg atattatcag
     2821 tgaaatcaag ggtggttatg ttatcaaggt agatggaaaa tactatgttt accttaagga
     2881 tgcagctcat gcggataatg tccgtacaaa agaagaaatc aatcggcaaa aacaagaaca
     2941 tagtcagcat cgtgaaggag ggacttcagc aaacgatggt gcggtagcct ttgcacgttc
     3001 acagggacgc tacaccacag atgatgggta tatcttcaat gcatctgata tcattgagga
     3061 cacgggtgat gcttatatcg ttcctcacgg cgaccattac cattacattc ctaagaatga
     3121 gttatcagct agcgagttgg ctgctgcaga agccttccta tctggtcggg aaaatctgtc
     3181 aaatttaaga acctatcgcc gacaaaatag cgataacact ccaagaacaa actgggtacc
     3241 ttctgtaagc aatccaggaa ctacaaatac taacacaagc aacaacagca acactaacag
     3301 tcaagcaagt caaagtaatg acattgatag tctcttgaaa cagctctaca aactgccttt
     3361 gagtcaacgc catgtggaat ctgatggcct tattttcgac ccagcgcaaa tcacaagtcg
     3421 aaccgccaga ggtgtagctg tccctcatgg taaccattac cactttatcc cttatgaaca
     3481 aatgtctgaa ttggaaaaac gaattgctcg tattattccc cttcgttatc gttcaaacca
     3541 ttgggtacca gattcaagac cagaacaacc aagtccacaa tcgactccgg aacctagtcc
     3601 aagtc
//
LOCUS       DAVLVZ010000102         3273 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000102 DAVLVZ010000000
VERSION     DAVLVZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.102"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            29..3148
                     /gene="phtE"
                     /locus_tag="VYN77_001931"
     CDS             29..3148
                     /gene="phtE"
                     /locus_tag="VYN77_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1559335.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPGN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEINLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKVQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNSE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1215 a          543 c          632 g          883 t
ORIGIN      
        1 cttcatcatt tattgaaatg agggatttat gaaattcagt aaaaaatata tagcagctgg
       61 atcagctgtt atcgtatcct tgagtctatg tgcctatgca ctaaaccagc atcgttcgca
      121 ggaaaataag gacaataatc gtgtctctta tgtagatggc agccagtcaa gtcagaaaag
      181 tgaaaacttg acaccagacc aggttagcca gaaagaagga attcaggccg agcaaattgt
      241 aatcaaaatt acagatcagg gctatgtaac gtcacacggt gaccactatc attactataa
      301 tgggaaagtt ccttatgatg ccctctttag tgaagaactc ttgatgaagg atccaaacta
      361 tcaacttaaa gacgctgata ttgtcaatga agtcaagggt ggttatatca tcaaggtcga
      421 tggaaaatat tatgtctacc tgaaagatgc agctcatgct gataatgttc gaactaaaga
      481 tgaaatcaat cgtcaaaaac aagaacatgt caaagataat gagaaggtta actctaatgt
      541 tgctgtagca aggtctcagg gacgatatac gacaaatgat ggttatgtct ttaatccagc
      601 tgatattatc gaagatacgg gtaatgctta tatcgttcct catggaggtc actatcacta
      661 cattcccaaa agcgatttat ctgctagtga attagcagca gctaaagcac atctggctgg
      721 aaaaaatatg caaccgagtc agttaagcta ttcttcaaca gctagtgaca ataacacgca
      781 atctgtagca aaaggatcaa ctagcaagcc agcaaataaa tctgaaaatc tccagagtct
      841 tttgaaggaa ctctatgatt cacctagcgc ccaacgttac agtgaatcag atggcctggt
      901 ctttgaccct gctaagatta tcagtcgtac accaaatgga gttgcgattc cgcatggcga
      961 ccattaccac tttattcctt acagcaagct ttctgcctta gaagaaaaga ttgccagaat
     1021 ggtgcctatc agtggaactg gttctacagt ttctacaaat gcaaaaccta atgaagtagt
     1081 gtctagtcta ggcagtcttt caagcaatcc ttcttcttta acgacaagta aggaactctc
     1141 ttcagcatct gatggttata tttttaatcc aaaagatatc gttgaagaaa cggctacagc
     1201 ttatattgta agacatggtg atcatttcca ttacattcca aaatcaaatc aaattgggca
     1261 accgactctt ccaaacaata gtctagcaac accttctcca tctcttccaa tcaatccagg
     1321 aacttcacat gagaaacatg aagaagatgg atacggattt gatgctaatc gtattatcgc
     1381 tgaagatgaa tcaggttttg tcatgagtca cggagaccac aatcattatt tcttcaagaa
     1441 ggacttgaca gaagagcaaa ttaaagctgc gcaaaaacat ttagaggaag ttaaaactag
     1501 tcataatgga ttagattctt tgtcatctca tgaacaggat tatccaggta atgccaaaga
     1561 aatgaaagat ttagataaaa aaatcgaaga aaaaattgct ggcattatga aacaatatgg
     1621 tgtcaaacgt gaaagtattg tcgtgaataa agaaaaaaat gcgattattt atccgcatgg
     1681 agatcatcat catgcagatc cgattgatga acataaaccg gttggaattg gtcattctca
     1741 cagtaactat gaactgttta aacccgaaga aggagttgct aaaaaagaag ggaataaagt
     1801 ttatactgga gaagaattaa cgaatgttgt taatttgtta aaaaatagta cgtttaataa
     1861 tcaaaacttt actctagcca atggtcaaaa acgcgtttct tttagttttc cgcctgaatt
     1921 ggagaaaaaa ttaggtatca atatgctagt aaaattaata acaccagatg gaaaagtatt
     1981 ggagaaagta tctggtaaag tatttggaga aggagtaggg aatattgcaa actttgaatt
     2041 agatcagcct tatttaccag gacaaacatt taagtatact atcgcttcaa aagattatcc
     2101 agaagtaagt tatgatggta catttacagt tccaacctct ttagcttaca aaatggccag
     2161 tcaaacgatt ttctatcctt tccatgcagg ggatacttat ttaagagtga accctcaatt
     2221 tgcagtgcct aaaggaactg atgctttagt cagagtgttt gatgaatttc atggaaatgc
     2281 ttatttagaa aataactata aagttggtga aatcaattta ccgattccga aattaaacca
     2341 aggaacaacc agaacggccg gaaataaaat tcctgtaacc ttcatggcaa atgcttattt
     2401 ggacaatcaa tcgacttata ttgtggaagt acctatcttg gaaaaagaaa atcaaactga
     2461 taaaccaagt attctaccac aatttaaaag aaataaagta caagaaaact caaaacttga
     2521 tgaaaaggta gaagaaccaa agactagtga gaaggtagaa aaagaaaaac tttctgaaac
     2581 tgggaatagt actagtaatt caacgttaga agaagttcct acagtggatc ctgtacaaga
     2641 aaaagtagca aaatttgctg aaagttatgg gatgaagcta gaaaatgtct tgtttaatat
     2701 ggacggaaca attgaattat atttaccatc gggagaagtc attaaaaaga atatggcaga
     2761 ttttacagga gaagcacctc aaggaaatgg tgaaaataaa ccatctgaaa atggaaaagt
     2821 atctactgga acagttgaga accaaccaac agaaaataaa ccagcagatt ctttaccaga
     2881 ggcaccaaac gaaaaacctg taaaaccaga aaactcaacg gataatggaa tgttgaattc
     2941 agaagggaat gtggggagtg accctatgtt agatccagca ttagaggaag ctccagcagt
     3001 agatcctgta caagaaaaat tagaaaaatt tacagctagt tacggattag gcttagatag
     3061 tgttatattc aatatggatg gaacgattga attaagattg ccaagtggag aagtgataaa
     3121 aaagaattta tctgatctca tagcgtaagg aatagcagta gaaaaagtct gaatcaaaaa
     3181 tgaagttctc tcaaaagtta gaaataaaac tctgactttg ggagaatttc attttattat
     3241 taatatataa aatttcttga catacaactt aaa
//
LOCUS       DAVLVZ010000103         3263 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000103 DAVLVZ010000000
VERSION     DAVLVZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.103"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            114..2765
                     /locus_tag="VYN77_001932"
     CDS             114..2765
                     /locus_tag="VYN77_001932"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1559336.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            2846..>3263
                     /locus_tag="VYN77_001933"
     CDS             2846..>3263
                     /locus_tag="VYN77_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV1559337.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDKDINVVSGDEVKEYLKIDTDDKLMLVACPPCQGFSTIGTNNENDERNQLV
                     FQFLRLINELNPDFLLMENVVGMTRTRNEKIFKKFLT"
BASE COUNT          950 a          684 c          742 g          887 t
ORIGIN      
        1 aattatacta tgtcaccact tgcagcattt gctgaaattg aagagattta tattgaactt
       61 tcaaaaattg gtttggttaa aattgtcaat aaaagtaatt aaggagaaca cacatgtcta
      121 aagaactttc acctaaatac aatccagccg aggttgaggc tggtcgttac caaaaatggc
      181 ttgatgcgga tgttttcaag ccttcaggcg atcaaaaggc taagccttat tcaatcgtga
      241 ttccaccacc aaacgtaact gggaaacttc accttggtca cgcttgggat acgactttgc
      301 aggatatcat catccgtcaa aaacgcatgc agggctttga tacgctttgg cttccaggta
      361 tggaccacgc tggtattgcg actcaggcta aggttgagga gcgcttacgt ggtgagggaa
      421 tttcccgcta tgaccttggt cgtgagtctt tcttgacgaa agtctgggaa tggaaagacg
      481 aatatgccac tactatcaag gaacaatggg ggaagatggg gctctctgta gactactctc
      541 gtgagcgttt cactcttgac gaaggtttgt caaaagctgt tcgtaaggtc tttgtggacc
      601 tttacaagaa aggctggatc taccgtggtg agtttatcat caactgggac ccagcagctc
      661 gcacagccct ttctgatatc gaggttattc acaaggatgt ggaaggtgcc ttctaccaca
      721 tgaattacat gctggaagat ggttcacgcg cccttgaagt tgctacaact cgtcctgaga
      781 ctatgtttgg ggacgttgcg gttgcggtta atccagaaga cccgcgctac aaggacttga
      841 ttggtaaaaa cgtcatcctt ccaatcgcta ataaactcat cccaatcgtt ggagatgagc
      901 acgcagatcc tgagtttggt actggtgtcg tgaaaatcac acctgcccac gatccaaatg
      961 acttcttggt tggccaacgt cataacttgc cacaagtcaa cgtcatgaac gacgacggaa
     1021 ctatgaatga gcttgccttt gaattttcag gcatggatcg ttttgaagct cgtaaggcag
     1081 tcgttgctaa gttggaagaa atcggtgccc ttgtcaaaat cgaaaaacgt gtccacagtg
     1141 ttggtcactc agaacgtaca ggtgtcgtag ttgagccacg cttgtctact caatggttcg
     1201 tcaagatgga ccaattggct aagaacgcca ttgccaacca agacacagag gacaaggtcg
     1261 aattctaccc acctcgtttc aacgatacct tccttcaatg gatggaaaat gtccacgact
     1321 gggttatctc tcgtcagctt tggtggggtc accaaatccc tgcctggtac aatgctgatg
     1381 gtgaaatgta tgtcggcgaa gaagctccag aaggtgacgg atggactcag gacgaagacg
     1441 tcttggatac ttggttcagt tctgccctct ggccattttc aaccatgggc tggcctgaag
     1501 tcgactcaga agactttaaa cgttattttc caacttcaac cttggtaaca ggttacgaca
     1561 tcatcttctt ctgggtgtct cgtatgatct tccagtcatt ggaattcaca ggccgtcaac
     1621 cattccaaaa cgtccttatc cacggtctta ttcgtgacga gcaaggacgc aagatgtcta
     1681 agtctctcgg taacgggatt gacccaatgg atgttatcga gaaatacggt gccgatgccc
     1741 ttcgatggtt cctttcaaac ggttctgcgc caggacaaga cgtgcgtttc tcttatgaga
     1801 aaatggatgc ttcatggaac tttattaaca agatttggaa catttctcgc tatatcctca
     1861 tgaacaatgg aggtttgacg ctggatgtgg cgcatgacaa tgtcacaaaa gttgcaacag
     1921 gtgaggctgg taatgtgacg gaccgctgga ttctccacaa tctcaacgaa accattgcaa
     1981 aagttactga aaactttgat aagttcgagt ttggtgtggc tggtcatatc ctttacaact
     2041 tcatttggga agaatttgcc aactggtatg ttgagttgac caaggaagtt ctttacagcg
     2101 acaatgaaga cgataaggta attactcgat ctgttctcct ctatacgctg gataaaatcc
     2161 ttcgtctcct tcactcaatc atgccattcg tgacagaaga aatctttggt cagtacgctg
     2221 aaggctctat cgtgacagca gcatacccaa ctgtcaaccc agcctttgag gaccttgctg
     2281 cccacactgg tgtggaaagt ctcaaagact tgatccgtgc cgttcgtaat gcgcgtgcgg
     2341 aagtaaatgt agcaccaagc aaacctatca caatccttgt taagactagc gatagtgact
     2401 tggaagcctt ctttaacagc aatgtcaact acatcaaacg ctttacaaat ccagaacatt
     2461 tggaaatcgc atcaaccatc cctgcacctg aactggctat gtcaagtgtc atcacaggag
     2521 cagaaatcta cctgccactg gccgatctcc tcaatgttga agaagaactg gcccgtctcg
     2581 acaaggaact tgctaaatgg caaaaagaac tggacatggt tggtaagaaa ctctctaacg
     2641 aacgcttcgt agccaacgcc aaaccagaag tcgtccaaaa agaatgcgac aaacaagctg
     2701 actaccaagc caagtatgat gcgaccgtag cacgtattga tgagatgaag aagttggtga
     2761 aatgatgtta aaaacctaac cctatcgggt tgggttttac atttgataat gttataataa
     2821 aaaggagaaa ctttaggaga atactatggg gaaaattgtt gctattgatt tatttagcgg
     2881 tgcaggtggc actacaagtg gccttaaaaa atcaggcata gatgttcaag ttgctgttga
     2941 aattgattcc gtagctgtta aaacttataa gttaaataat cctgaggtgt ctgtaataga
     3001 taaagatata aatgtagtat caggagatga agtaaaagag tatcttaaaa ttgatacaga
     3061 cgataaacta atgctcgtgg catgcccacc atgtcaaggt ttttcaacta ttggtactaa
     3121 taatgaaaat gacgagagga atcaacttgt ttttcaattt cttcgtttga ttaatgaatt
     3181 gaatcctgat tttctattaa tggaaaatgt tgtgggtatg actagaacaa gaaatgaaaa
     3241 aatctttaaa aaatttttga cat
//
LOCUS       DAVLVZ010000104         3132 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000104 DAVLVZ010000000
VERSION     DAVLVZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.104"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            14..>3132
                     /locus_tag="VYN77_001934"
     CDS             14..>3132
                     /locus_tag="VYN77_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1559338.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIEIKIDFQEEI
                     QENPDLVEGIVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPIPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALSKAVITDKG
                     ETEVQPESPDTVVSDKGEPKQVAPLPEYTGPQASAIVEPEQVAPLPEYTGVQAGSIVE
                     PEKVEAPKEYTGKIEQPSAEDTKPENGASSTNGESERPKDKIKEEKQVDKKLELRNVS
                     NVELYTVENNKYRHITAVDGALDSSLKYFMKVKSENFKDIMLPVTKIESTTKNNKEVY
                     KIVAHAENLIQHENNVISNDYTYYLPKTQQSETGVYTSFKNLVDAMNSDPNGTFHLGA
                     TMDAREVELPDDQESYVKNEFYGKLIGKNNGKYYAIYNLKKPLFKTLNTATIQNLSIK
                     EANVSSKEDAATISKEAKYNTLIDNVHSDGIIAGERGIGGLVSKVDNSRISNSSFTGR
                     ITNTYDTTAGYEIGGLVGKLSGSLASIEKSIASIDIASNAKSGDQIVGGIAGVVEKSA
                     TIKYSYVEGNVNNVRHFGKVGGVAGNLWDRDSQDVSKSGKLSYVLSDVNVTNGNAIAG
                     YNFNGIKTIETYSNKNNKVVNVVQEDDEVVTKDSDVQRGTVLDADKVKEKKVELVSKH
                     STKVEDFDFTSRYNTNYNEVTGYQQSREQVYKNIEKLLPFYNRETIVKYGNLVEDNSD
                     LFTKKLLSVV"
BASE COUNT         1206 a          455 c          625 g          846 t
ORIGIN      
        1 tcaaggagga ataatggaaa agtattttgg tgaaaaacaa gagcgttttt catttagaaa
       61 attatcagta ggacttgtat ctgcaacgat ttcaagttta ttttttatgt ctgtattagc
      121 tagttcatct gtggatgctc aagaaactgc gggagttcac tataaatatg tggcagattc
      181 agagctatca tcagaagaaa agaagcagct tgtctatgat attccgacat acgtggagaa
      241 tgatgatgaa acttattatc ttgtttataa gttaaattct caaaatcaac tggcggaatt
      301 accaaatact ggaagcaaga atgagaggca agccctagtt gctggtgcta gcttagctgc
      361 tctgggaatt ttaatttttg ctgtttccaa gaaaaaggtt aagaataaaa cggtattaca
      421 tttagtattg gttgcgggaa taggaaatgg tgtcttagtt tcagtccatg ctttagaaaa
      481 tcatcttttg ctaaattaca atacggacta tgaattgacc tctggagaaa aattacctct
      541 tcctaaagag atttcaggtt acacttatat tggatatatc aaagagggaa aaacgacttc
      601 tgattttgaa gtaagtaatc aagaaaaatc agcagccact cctacaaaac aacaaaaggt
      661 ggattataat gttacaccaa attttgtaga ccatccatca acagtacaag ctattcagga
      721 acaaacacct gtttcttcaa ctaagccgac agaagttcaa gtagttgaaa aacctttctc
      781 tactgaatta atcaatccaa gaaaagaaga gaaacaatct tcagattctc aagaacaatt
      841 agccgaacat aagaatctag aaacgaagaa agaggagaag atttctccaa aagaaaagac
      901 tggggtaaat acattaaatc cacaggatga agttttatca ggtcaattga acaaacctga
      961 actcttatat cgtgaggaaa ctatagagat aaaaatagat tttcaagaag aaattcaaga
     1021 aaatcctgat ttagttgaag gaattgtaag agtaaaacaa gaaggtaaat taggtaagaa
     1081 agttgaaatc gtcagaatat tctctgtaaa caaggaagaa gtttcgcgag aaattgtttc
     1141 aacttcaacg actgcgccta ttccaagaat agtcgaaaaa ggtactaaaa aaactcaagt
     1201 tataaaggaa caacctgaga ctggtgtaga acataaggac gtacagtctg gagctattgt
     1261 tgaacccgca attcagcctg agttgcccga agctgtagta agtgacaaag gcgtaccaga
     1321 agttcaacca gcgttgtcca aagcagttat aaccgacaaa ggtgagactg aggttcaacc
     1381 agagtcgcca gatactgtgg taagtgataa aggtgaacct aaacaggtag caccgcttcc
     1441 agaatataca ggaccacaag ccagcgctat tgttgaacca gagcaggtag caccactacc
     1501 agaatacaca ggggtacaag ccggatcgat agtcgagcca gagaaagtag aagcaccgaa
     1561 agaatatact ggtaaaatcg aacagcctag tgcagaagat actaaacctg aaaatggggc
     1621 aagctctaca aatggggaat cggaacgtcc taaagataaa attaaagaag aaaaacaagt
     1681 cgacaagaaa ctagaactta gaaatgtttc aaacgtggaa ttatatacgg tagaaaataa
     1741 taaatatcga catattacgg ctgtagatgg tgctttagat agttcattga agtatttcat
     1801 gaaggtaaaa tcagaaaatt ttaaagatat aatgttgcct gttactaaaa ttgaaagtac
     1861 aacaaaaaac aataaagaag tatacaaaat tgtggctcat gcagaaaatc taattcaaca
     1921 cgaaaacaat gttatttcga atgactatac atattattta ccaaaaactc agcaaagtga
     1981 aactggcgtc tatacatcat ttaaaaattt agttgatgca atgaatagtg accctaatgg
     2041 aacattccat ttaggtgcaa ctatggatgc tcgtgaagtt gaacttccgg atgatcaaga
     2101 aagttatgta aaaaatgaat tttacggaaa attaataggt aaaaataatg gtaagtatta
     2161 tgctatttat aacttgaaaa aacctttatt taaaactcta aacaccgcta ctattcagaa
     2221 tctttcaata aaagaagcga atgtttcttc taaagaagat gctgctacaa tatcgaaaga
     2281 agcaaaatac aatactctta tagataatgt tcattctgat ggtattatag ctggtgaacg
     2341 tggaattggt ggattggttt caaaagtaga taactcgaga atttctaata gtagttttac
     2401 aggcagaata actaacacat atgatacaac tgcagggtat gaaattggtg gtttagtagg
     2461 taaactttct ggttcgcttg cttctataga gaaatcaata gcatcgattg acatagcctc
     2521 taatgcaaaa tcaggagatc aaattgttgg tggtatagcc ggagttgttg agaaaagtgc
     2581 gacaattaag tatagctatg ttgaaggaaa tgttaacaat gtgcgacact ttggtaaagt
     2641 tgggggagtt gcaggtaatc tttgggatag agattcacaa gatgtaagta aatcaggtaa
     2701 actatcatat gttttaagtg atgtcaatgt tacgaatggt aatgctatag ctggctataa
     2761 tttcaatgga ataaaaacga ttgaaacata tagcaataaa aataataagg tagtaaacgt
     2821 agtacaagaa gatgatgagg ttgtgactaa agattcagat gtacaaagag gtacggtatt
     2881 agatgctgat aaagtaaaag agaaaaaggt agaattggtt tctaaacaca gcactaaagt
     2941 agaagatttt gattttactt caagatacaa tactaattac aatgaagtaa caggttacca
     3001 acagtcgaga gaacaagtat ataaaaatat tgaaaaatta cttcctttct ataacagaga
     3061 aacaatagtt aagtatggta atttagtaga ggataacagt gacttattta ctaaaaaact
     3121 tctatcagtt gt
//
LOCUS       DAVLVZ010000105         3074 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000105 DAVLVZ010000000
VERSION     DAVLVZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.105"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..102)
                     /locus_tag="VYN77_001935"
     CDS             complement(<1..102)
                     /locus_tag="VYN77_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1559339.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSN"
     gene            351..2939
                     /locus_tag="VYN77_001936"
                     /pseudo
     CDS             351..2939
                     /locus_tag="VYN77_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          936 a          455 c          679 g         1004 t
ORIGIN      
        1 attagaattg tttatcccaa atttatttga aagcttctct aagctatatc cttgttttct
       61 aagttcatag atctgaactt tatcatcata agttaatttc ataataaaaa caccccaaaa
      121 agttagattt tttctgtcta acttttgggg tgtagttcaa aacaacattg gtaatatgcg
      181 ttttttgaat tttatagact gctcgtttaa actctattta cctcgtacat tccggagcga
      241 gatggactat tagtcgcata caaaatgagt gctattcgga ttttattttc gtgtacaatt
      301 tcagctattc ttgttataat cagcctatag gaatcaagga ggtgactctt atggctgttt
      361 ttgtgtcttt ggatggaatt gtggtagaag tacttgatgt cttttcttct tttgatgggg
      421 atagtgagtt tttcttgtgt aaacgtctta aagacaagag tcagtttgtt atggaacgct
      481 ctcagttcga ggagatgttc caacttcaaa gtagtcgctt gacgacgcaa gaaaaattac
      541 aattgtttac ctctgtgttt gctggccgtt atgatgttta tgctaagagt tttatcaatg
      601 atcaagagaa aattcagtat tttccttcct atgattatgg ttggaagcag ttgctacctg
      661 aaaaacggag tttccagaca ttgacggatt ccgttttgaa atctcatttt cgtggggaga
      721 cagctatcgg tatctttcct atgcacttag atgatagctg tcattttttg gtactggatt
      781 ttgatgaagg agattggaaa gaagctggtt taaccattcg aagaatagcc agggaacgcc
      841 agatggaagc ccatttagag atttctcgtt cgggttacgg actccatatt tggttcttct
      901 ttgaggaagc gattctgagt cgaaaggctc gtctgtttgg aaagaaacta ttagaattgg
      961 ctatgcagga aagtatgcaa ctgtcctttg attcttttga tcgcatgttt ccaaatcagg
     1021 atgttcttcc taaaggtgga tttggtaatt taatttcctt tccttttcaa ggagaagctt
     1081 atcatcaagg gcgaacggtc cttgtggatg aacattttca accttatgga gaccaatgga
     1141 ggtatctgca aggaattcag aagatttcaa ctgctaaagt ggcactgtta atccaagagg
     1201 agttaggcaa gcaagaattg gataaggagt tgaaggtcgt tttatccaat atgatccaac
     1261 ttaaaaaatc gtctgtgaca cccaagacac tgtttttctt gaaaaatatg gcttcatttt
     1321 ctaatcccga attttattta aagcaggcta tgcgacagcc aaactatcaa attcctgagc
     1381 gaatgtattt atttggagaa tccgattatt atttatggct gccaagaggc ttgttatatc
     1441 cattgcaaga taaatttaag caggtagttg tggaggatag gagaaaagta caaaggtcta
     1501 ttagagtagc atttaaggga gaactcactt ttgagcaaga gttagccctg tcagatatga
     1561 cttctaaaga aaatggttta cttcatgcgg gacaggtttt ggaaagagcg ttttaggtgc
     1621 tgctcttatc tctgaacgta agacaaaaac cattatccta gttcataata ggcaactctt
     1681 agaccaatga ctggatcgtt taaagtgttt tttgactttc gaagaggcag aagctacccg
     1741 ttatacacca tcaggtcgtg aaagggtaat aggctatgtc gggcagtacg gtggggctaa
     1801 gaaatgactg agtaaactgg ttgatgttgt tatgattcaa tctctattta agttggaaaa
     1861 tagtcaaagt cttttggatg agtatgagat gatgattgtg gatgagtgtc atcatatctc
     1921 tgccttgatg tttgaaaaag ttgttgctca gtttagaggg aagtatcttt acggtttgac
     1981 ggctacgcct gagcgtaaga atggtcatga acctattgtt tttcagagaa attgaaaaga
     2041 ccaaagcaag taattttata cagcccagtg attggattgc tacagactca gcgaggaatc
     2101 agttgattct caaggatatt ctagcccaag tggcagaagg acggaatatc ttggttttag
     2161 ttaatcgaat tcaacagata gatgtctttg aaaagttatt gaaagagaaa gaggttgatg
     2221 actgttacat tattagcgga aaaaccaaag tccgagaaag aacgagttta ctggagacgt
     2281 tagaacagtt agataaaggg tttgttttgt tgtctactgg aaaatacatt ggcgaaggtt
     2341 ttgacttacc tcagttggac acgcttatct tggcagcacc cttttcttgg aaaaataatt
     2401 taattcagta tgcaggtcgg attcatagaa actacaagga taagtctttg gtgcgtattt
     2461 tcgattatgt ggatattcat gttccttatt tagaaaagat gtttcagaaa cgacaagtag
     2521 cttatcgaaa gatggattat cgtgtcatcg agggtgagga gaaacaattc gtttatgttg
     2581 atagtagata tgagaaggtg ttgagagagg acttagcagg ggaaagacag gaatgtctgc
     2641 ttattttacc ttatgtgcac cagacaaaac tgatgaattt tctaaaagaa tttaggatta
     2701 gtcaaattga gatatgtata ccagagacgg ttgcaaataa agcatggcta gaccagttga
     2761 agagccagaa aattaaagtg tcttttactc aatcaaaaat agtaacgcct attcttttgg
     2821 tgaataagac tattgtttgg tatggtgcaa tgccattatt agggaaggta gatgagatga
     2881 ccatattacg tttggaatca gctagtatag tttctgaact agtggcaggt ttacgataga
     2941 gaaaattttt aaaaatttct atgtatgatt ttcatttctt tagtgagact gttgccatta
     3001 tcacattcga atcacacaaa ataaaaaaat ttttataagt acttgacaaa tagattgaaa
     3061 tatcataaaa tgaa
//
LOCUS       DAVLVZ010000106         3047 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000106 DAVLVZ010000000
VERSION     DAVLVZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.106"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(203..931)
                     /locus_tag="VYN77_001937"
                     /pseudo
     CDS             complement(203..931)
                     /locus_tag="VYN77_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(1191..1502)
                     /locus_tag="VYN77_001938"
     CDS             complement(1191..1502)
                     /locus_tag="VYN77_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559340.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHNS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1517..2803)
                     /locus_tag="VYN77_001939"
     CDS             complement(1517..2803)
                     /locus_tag="VYN77_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1559341.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFATKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDVY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          915 a          662 c          527 g          943 t
ORIGIN      
        1 ttattaattt ttttcaaaat atctaagaca tcacttcttt gtttaatttg ataggctttg
       61 atacctaagc tccgacttta tcacttaaaa aataaaaaag gacagctaat ctcttgcgca
      121 tcaagggatt tcgccattct ttaatctcgt ttaagtgttg tatatggagc tttgcataat
      181 tttttaaaac taaacaacta cattaagcac gaactgtgac gctggttcca tcttccttat
      241 aaagattgat gagtccttct tttaatgcac gaaccatgtc tccagcttga acaggttgag
      301 gcaccttaat agtcaatagt tccattggat ttggagcgcg gtcgatttta ttgcctttgg
      361 catcatgcaa atcttcaata taggtttcaa aatgacggaa acctggacca taaaactcaa
      421 cttggtcccc ttcgttaatg acgtttcgtt gacgaatagt tgctgtttgt gccgcatcat
      481 cataagaaac cacttcagcg acaaacttgt actcagggat tttacgacga gcaccaaaca
      541 actgctcatt ttcagatggt gtaccatagt aaaatcctgt agccagttca cgttgggcaa
      601 ccttccacat ctcgtccacc aagtcttgtt tgatagcttc aaacttttca ggactttcaa
      661 gataggcatc cacagccgcc ttgtagcagt tggttactgt taatacatag tgaatagact
      721 ccatacgtcc ttcgattttt agactgtcca caccattttc aatcatatct gagatatggt
      781 caatcataga catatcaaca gctgacattg aaaattcttc tggaatctca ccctgcaaac
      841 tcttacgttc tttcccaaat ggcatatcgt aaaggtcgta cttccaacgg catgactgag
      901 aacatccacc acggttggca tcacgcatac tctagacgga ttgttttgac aattctaacc
      961 ttttagactt tgcataataa ctgttaaaca gtcgaaaatt accccagttt taacaagtag
     1021 tcaaaatacc agataaagta aaaaggggct aggaaaaaag tctttaaaat cttaaaaacg
     1081 catagtatca ggtattgaac aatcttgata ttatgcgttt tattgtggga agatttactt
     1141 cattttctct tggaattgag ttttctccta cttcaaaact agatgatttg ctatttaaaa
     1201 tagcgttcct tatgattttg gcaacctgga ttaaagcgag aaaaacaaaa gaggcaactt
     1261 aagctttctt tatattgctt atatgttaat agttttagac aaacaccaca taaaataggc
     1321 ttatcctgta tcaaagataa gggataaggc tcaaaagggt gattttctaa tgaattatga
     1381 caccgataac aagcataata ctttttacaa tcatagcatt gtaaagcaat aatatccaac
     1441 ttgctatggt aatgaatgca tcttgtttgc ttatccacta agaaaccctg tgcctctatc
     1501 atttcctttt cctttcttaa gctcgaactg tgacgctggt tccatcttcc ttataaaaat
     1561 tgatgagtcc ttcttttaat gcacgaacca tgtctcctga ttgaacgggt tgtgggactt
     1621 taatagtcaa tagttccatt ggatttggag cgcggtcgat tttattgcct ttggcatcat
     1681 gcaaatcttc aatataggtt tcaaaatgac ggaaacctgg accataaaac tcaacttggt
     1741 ccccttcgtt aatgacgttt cgttgacgaa tggttgctgt ttgtgccgca tcatcataag
     1801 aaaccacttc agcgacaaac ttgtactcag gaattttacg gcgagcacca aacaactgct
     1861 cattttcaga tggtataccg tagtagaaac ctgttgccaa ttcacgttgg gcaaccttcc
     1921 acatctcgtc caccaagtct tgtttgatag cttcaaactt ttcaggactt tcaagataga
     1981 catccacagc cgccttgtag cagttggtta ctgttgatac gtagtgaata gacttcatac
     2041 gtccttcgat ttttagactg tccacaccat tttcaatcat atctggaata tggtcaatca
     2101 tagacatgtc aacggctgac attgaaaatt cttctggaat ctcaccctgc aaactcttac
     2161 gttctttccc aaatggcata tcgtaaaggt cgtatttcca acggcatgac tgagaacatc
     2221 caccacggtt ggcatcacgc atactcatgt ggtttgaaag agtacaacgt ccagagtatg
     2281 aaatacacat agctccatgg acaaaggctt caatttcaac atctgtacgt ttgcggatct
     2341 cagctaattc ttccattgaa acctcacgcg ctaaaacgac acgagtcaag cctagctctt
     2401 tccagaactc aagggtttca tagttagtgg cactggcttg ggtagaaagg tggatttcaa
     2461 ggcctggtgc ttcagtcgct gcaatcataa tcaaggctgg gtcagatacg ataactgctg
     2521 caatcccgat atcacgcagt ttacggaacc actcaccagc accagcttca tttccttcgt
     2581 gcataaccat attagccgct acatagacct tggcaccata cttggtcgca aactgcacgc
     2641 cttcttccat ctgttcgaaa gtaaagtttc ccgcacggct acgaagacca taggcctgac
     2701 caccgataaa gacagcatct gctccatact gaacagctac ctttagcttc tctaaagtcc
     2761 ctgcaggtga taaaacctca ggacgtttta atgtttttgt catgatttct cctatttgca
     2821 atataaaagt ttgacatttt tccttctcat tttatagtaa ataaaggata aaatcaagcc
     2881 tagacggatt gttttgacaa ttctaatctt ttagactttg cataataact attaaacagt
     2941 cgaaaattac cccagtttta acaagtagtc aaaaagtcta aaaaagtaaa aaccagtatc
     3001 ccaagatact gactcgtaaa aatatataaa ttagtcctta gatgact
//
LOCUS       DAVLVZ010000107         2954 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000107 DAVLVZ010000000
VERSION     DAVLVZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.107"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VYN77_001940"
     CDS             complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VYN77_001940"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1559342.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1420..2196
                     /gene="recX"
                     /locus_tag="VYN77_001941"
     CDS             1420..2196
                     /gene="recX"
                     /locus_tag="VYN77_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1559343.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2285..2818
                     /locus_tag="VYN77_001942"
     CDS             2285..2818
                     /locus_tag="VYN77_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1559344.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          947 a          541 c          530 g          936 t
ORIGIN      
        1 cttttcaaac tttgtcaact acttttttaa acttttttta ttaattttac aacagcttca
       61 gttcgagctg tatgtgggaa catatcgacc gactggatat aatgaagatc atagacttct
      121 actaagcgta ccaaatcacg agccaaggtc gaaacattac aagaaatata aaccattttt
      181 tctggtacat aagtaagaat agtatctaat aacttatcat ccagacctgt acgtggtggg
      241 tcaacaatca aagcatctgc tcggtagcct tccttgtacc aacgaggaat aatctcttct
      301 gccgttccag cttcataatg agtattgtca aatcccattc ttttagcatt tcgcttggca
      361 tcttcaatag cttctggaat aatatccata cctctgagtg tttttacttt ctttgcaaag
      421 gcaaatccaa tcgttccaac tccacaataa gcgtcaatca aatggtcttc tttatcaaca
      481 tccagcgctt ttactgcttc gctatagagg acttctgttt gctcaggatt tagttgataa
      541 aaagctcgag gggatagtga aaattcataa ttgagtacac cttcttgaat actctcttgc
      601 ccccagataa tctctgtctt ttcaccatat atctcactgg ttttagctgt atttgtatta
      661 acagctactg tcacaacttc tgggaaatct ttaaccaact cttttaccaa ttgagttaaa
      721 ttaagctggc ggtttgtaac aataataatc tgaacctgtc cggtctttct cgcgcgtcgg
      781 accataatag tacggacacc tagaactttt ctctcatccg tgattggaat ctggtgataa
      841 gtaagtaatt ctgctaagcg attagcaatc acttgggttt ccttatcttg taccaggcag
      901 tctttcaact ctactaaata gtgagagttt tgtgcatata agcccgcctt gacctgattt
      961 ttaaattttc gagtctgaaa ttgtaactta gctctgtaat attttggttc ctgcattcca
     1021 atagttggac gaatttcata attttcatat cctgcaggag caaatttttt cagcgcttga
     1081 tgaagtaagt ccgtcttgaa ctccagctgc ttatcataat gcaggtgcat gatttggcag
     1141 cctccgcatt cattataaat agtacaagat ggcacaattc gaaatttaga cttcttgttg
     1201 accttcagta attttgcttc aacaaagttg cgtctaatag aagtaatctg acaatagata
     1261 tcttcgcctt tgagagctcc tggtacaaag actaatgttt tttggtaaaa gccgattccc
     1321 tcaccgttaa ttcccatgcg cttgattttt aatggtattt tttgtttcac tttcagattc
     1381 atacccctat cttatcacat tttgagttat aatagagcta tgaaaatcac aaaacttgaa
     1441 aagaaaaaaa gactctatct gatggagctt gataatggcg acaaatgcta tatcaccgaa
     1501 gatacaattg ttcgttttat gttatcgaga gataaggtga taagcgaaga ggaattgaaa
     1561 gagattcaag actttgctca attttcttat ggtaagaatc tcgctctcta ccacttatcc
     1621 tttaaagcac gcactgaaaa agaagtcaga gaatatctga aaaaatacga tattgataaa
     1681 aacatcgttt ctcaagttat tgctaatctt aaagaagata agtggattaa tgatggtcag
     1741 tacgcttatg ctatcatcaa taccaatcaa ctttcaggag acaagggtcc ttatgtgctg
     1801 actcagaaac tatctcaaaa agggatttca aaatctacta tagaagagaa cttgaaagaa
     1861 tttgattttt ctgaagttgc tcaacgtgta gccaataaac tattgaaaaa atatgaggga
     1921 aaacttccag ctcgtgccct gcaagataag attatccaga acttgactaa caagggcttc
     1981 tcttattctg atgctaaaat tgcctttgac gacttagata gccaagttga ccaagaaacg
     2041 actcaagaac tcatcttcaa ggaacttgat aagcaatata ctaagtatgc tcgaaagtat
     2101 gaaggatacg aactgaagca gcgtttaact caagttttag cacgaaaggg ctacgatttt
     2161 tcggatatag caagcgctct tagagaatat ctttaatatt tccaagtgaa attcacagat
     2221 tttaggtaat tttatggtac aatagtaaat gataaactta taaattgtag aaagttggtt
     2281 agttatgaag cttccaaaag aaggcgactt tattacaatt caaagttata agcatgatgg
     2341 gagtctccac cgaacttggc gggacaccat ggtactaaaa acaacagaaa acgccattat
     2401 tggtgtcaac gatcatacac ttgttaccga aagtgatggt cgtcgttggg tcactcgaga
     2461 accggctatt gtttactttc acaagaaata ttggtttaat atcattgcca tgattcgtga
     2521 taatggaact tcttactatt gcaatatggc tagcccctac tatctggatg aagaagcact
     2581 gaagtatatt gattacgatt tggatgttaa aatttttaca gatggggaaa aacgtctctt
     2641 ggacgttgaa gagtacgagc gtcacaaacg caaaatgaat tattctgatg acttggacta
     2701 tattttaaaa gaacatgtca aaattcttgt tgattggatt aacaatggac gtggtccttt
     2761 ctcagaagcc tatgtaaaca tttggtacaa gcgctatgta gaactaaaga atcggtaaag
     2821 ttgtcaaagg tcaggaacaa aatcctgacc tttttttggt agtataaaat aaattgtgta
     2881 aacacaaaaa ggaataaatc tattatagca gagttacaac acattactag aaagagattt
     2941 atgcaaaatt cgta
//
LOCUS       DAVLVZ010000108         2921 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000108 DAVLVZ010000000
VERSION     DAVLVZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.108"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(105..2363)
                     /locus_tag="VYN77_001943"
     CDS             complement(105..2363)
                     /locus_tag="VYN77_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1559345.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLKEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2592..2822)
                     /locus_tag="VYN77_001944"
     CDS             complement(2592..2822)
                     /locus_tag="VYN77_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1559346.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
BASE COUNT          788 a          603 c          605 g          925 t
ORIGIN      
        1 actatatatc ataatggaca aagggcaagc tgtcaagaaa ttacagttat tttagtaaaa
       61 aaagaagcag aaaaatttca ttttctactc cttttctata ggatttattt ttctttttca
      121 gaacttttaa cttcagcttt tttggcagat ttaatctgaa tgtttccctt gctagtcata
      181 actgctttga gatctttttc gcttggattt tcaaggtagt agtcagtgat tgtgtcctca
      241 atatagtctt gaatagtccg acgaagtggg cgtgctccca tttttggatc ataacctagg
      301 tcaaccaact tttccttgac cttatcagtt acatccaaac gaatgttgtt gctagagagg
      361 cgcttgttaa catctgctag catgagctcg acaatctgaa ggaggttatc cttgctgaga
      421 gccttaaatt cgataatgcc atcaaaacgg ttcataaact ctgggctaaa gaagttaccg
      481 agttcaccga ggacagaatt ggtacgtcct tctctagcag caccaaatcc aacgctagct
      541 tcggtctttc ctgtacctgc atttgaggtc atgataatga tggcatcctt gaagctaacg
      601 gtgcgtcctt gcccgtctgt caaacgacca tcgtccaaga cttgaagaaa catgtgcata
      661 acatctgggt gagctttttc cacttcatcg agaaggatga gagaatatgg attgtggcga
      721 actttttcag ttaattgacc agcctcatca tagccaacat aacctggagg ggcgccgacc
      781 aacttagcca cactatgttt ttccatgtat tcactcatat caaagcgaat catactatca
      841 gcagaaccaa aaagttcgat agccagttgt ttggaaagtt ctgtcttacc gacaccagtt
      901 ggcccaacga agaggaagct tccgattggg cggttagggg taccaagtcc gacacgatta
      961 cggcgaatag ccttggcaat cttatcgact gcatcatctt ggccaataac atgagacttg
     1021 agatcttcgg ctagatggat gagttgagat tgttctttct ctttcaaatc accaacaggg
     1081 atattggttt tctgctcgat aatgtgctca atagttttct cgctgatgat aggagtatcc
     1141 tggtctgtga tctttttctt ttgcatttcc ttatacttgg caatctggtc gcggaagtag
     1201 gccgccttct caaaatcttc ttctcgtgta gcttgagact tgagattttc agcctcaatc
     1261 aagcgctgat caattacttt aggatccaca aaattcaagg tcaagttcat cttagaacca
     1321 gcttcatcta agaggtcaat ggccttgtca ggcaagaagc gatcttggat gtagcgattg
     1381 gaaagagttg cagctgcttc aatcgcagca tcggtatatt gaacgtggtg gtaatcttcg
     1441 tatttctttt gaatcccttt gagaatagtg attgtttcat ccaccgttgg ttcatcgact
     1501 ttaacaggct gcatacgacg ctcgagggca gcatcctttt caatgatacg gtattcattg
     1561 agggtagtag caccgactag ttgcagttct ccacgagcaa gggctggctt gaggatattt
     1621 cctgcgtcca tattaccatc actcgcagaa ccagcaccaa caatttcatg gatttcatcg
     1681 ataaagagga tgatgtcttc acgtttgcga atttcttcca tgagtttttg catgcgttct
     1741 tcaaattgtc ctcgaatccc cgttccttga actaagctaa ccacatccag acggatgact
     1801 tgtttacctt ggagtttatg tggcacatcg ccatcgacaa ttttctgagc tagaccttcg
     1861 acaacggccg tttttccgac accaggttca ccgataagga caggattatt cttggttcta
     1921 cgattgagaa tctcgatgac acggataatc tcatcgtcgc gcccaataac ggggtcaatg
     1981 tctccacgac gggcaatttc agttacatta ataccaaatt ctttcagcag gcctttttct
     2041 tggctaggtg gcggagtttg agcagatcca cgattttggg aaccataacc gccgtttcca
     2101 ccgtaacctc cacctgattg ggttggggga ataggaggag tattgctaga aggtctgaaa
     2161 ttgtttagat cattgaagaa atcaccaaag ggatcgaagt cacgattgtt cagatccgtc
     2221 atacctttga agaggctatt gttaggatct gttttgataa tcttatagca gttttgacag
     2281 aggtcaattt gtttttgttt tccattgaga ttggtgtaaa gatgaattgt tgagtcgtta
     2341 attttacagt tttgacaaag catacatcta cctcatttct ttctttagcc tgacttgttt
     2401 aaaggtcaaa aatagtcaaa ttctatactc tcattataac aggattgggg tgtaaagcaa
     2461 gtaaaaagat tgaagctttg agaagttgtt acaatgaaaa tttttgtgaa tttggactat
     2521 aaaaaaatcc tatttgtgag acaaaaagga ttttacttag acatgcaggg acaatcccag
     2581 cttgttttag attagtagag aagttcgtaa atttccattg cgattaagtc aatgctatca
     2641 aacgtatacg tttcgcgatc ttctacgtca cgaagggtaa agattgggcc gttttcttcg
     2701 tcgtggctgt acgcaacttc tgcaacaaca actccttcgc gttcaaaatt acgttttaaa
     2761 tttccgccat cgtttgccat tgcttcaagg cggttgatga ttctaaccaa atgtgattcc
     2821 attttgattc tccttcattt cttatcgtta ctattttacc ataaaggagg ccaacttgac
     2881 tagaaaaaac taagatttct cgctaattct gctagtttaa a
//
LOCUS       DAVLVZ010000109         2893 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000109 DAVLVZ010000000
VERSION     DAVLVZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.109"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(85..1026)
                     /locus_tag="VYN77_001945"
     CDS             complement(85..1026)
                     /locus_tag="VYN77_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1559347.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(1004..2767)
                     /gene="aspS"
                     /locus_tag="VYN77_001946"
     CDS             complement(1004..2767)
                     /gene="aspS"
                     /locus_tag="VYN77_001946"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1559348.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          844 a          677 c          583 g          789 t
ORIGIN      
        1 agatagaact gacgaagtca gtaacataca tacggtaagg cgacgctgac gtggtttgaa
       61 gagattttcg aagagtatta atcactaatt atctatctca acaaatcttc ctagaatatg
      121 aacattttcc gagacagaga caaaggagct tggatccact tgtgtcataa tctgtttaaa
      181 ttcattaaac tctgcacgtg taatgacagt gattaaaact gcctttctct cgtgattata
      241 ggttccttct gcatcgtgga tcatggttgc tccgcggtgc aattttttat ggattttttc
      301 aattaccttc tctggatgat ttgtcacaat catggcctgc atacgctttt gcttagtaaa
      361 gactgcgtct gtcacacggc tagagacaaa gatggtaatc atagaataaa gagcgtattt
      421 ccaaccaaag gtcaaacctg ctatcagcat gatagttcca tttaccaaga aagaaatact
      481 accgacattc ttacccgttt tcttgcgaat agtcaggctg acgatatccg tcccaccact
      541 ggaaatattg tttcgaagag caaaaccaat ccccaaaccc ataacaacac ccccaaaaag
      601 ggaattgata atgggatcct ccgtcaaggt tgccacaggg acaaactgga taaagaagga
      661 actcatagat accgtgataa aggtaaagac ggtgaactta tggccaatct gataccaagc
      721 taagaccatc aaagggaagt taatggcgta gaagcttagc gaaatcggaa tatgaaaacc
      781 aaaccagtga ttactcaagg cagagataat ctgtgccaga cctgttgcac cactcgaata
      841 cacatgccct ggttggaaaa agaaattaac tgctactgct gataaaaaac catagaccag
      901 agaggccgaa atcttctcat catacttctc tcgagagata ctttgtaaga cacgtaaaat
      961 ttttatctga taagcaaagc ggcgcagata atagcgccac cgcttaattc gttttgtttg
     1021 tttcatcttc ttctacttgt aagctgagtt cctctagttg tttgagagcg actgttgatg
     1081 gagcttgtgt cattgggtca gttgccttgt tgttcttagg aaaggcaatg acttcacgga
     1141 tattttcttc tccagcaagc aacatgacaa aacggtcaag cccgatagcc aaaccaccgt
     1201 gtggtgggaa accatagtcc atggcttcaa gaaggaaacc aaactggtca ttggcttctt
     1261 cagttgagaa accaagagcc ttgaacatgc gttcttgaag gtctttttgg ttgatacgaa
     1321 ggctaccacc accaagctca taaccgttca agacgatatc gtaagcaatg gcacgaacct
     1381 tagccaaatc accttctaat tcgtgagcag tctcttcctg tggaagtgtg aatggatggt
     1441 gggcgctcat gtagcggcct tcttcttcag accattcaaa catcggccag tcaaccaccc
     1501 aaaggaagtt gaacttatca ttatcaatca agccaagctc tttagcaata cgtccacgaa
     1561 gggcacccag tgttgcatta gccacttcaa gcgtatccgc cacaaagaga accaagtcct
     1621 tatcttcaag agcaagcgct gttgtcaatt cttcttggat accagtcaag aacttggcaa
     1681 ctggtccgtt taattctcca tcaaccacct tgacccaagc aagacctttg gcaccatact
     1741 gtttggctac ttccgtcatc ttgtcgatgt ctttacgtga atagttgtcc gcagctcctt
     1801 tgaccacaat cgcttttaca gcaggtgctt ctgaaaagac tttaaagtct acacctttga
     1861 ccacttctgt caagtcctga agcaacatgt caaaacgagt atctggcttg tcagaaccgt
     1921 aaagagccat agcatcatcg tatttcatac gagggaacgg tagcgttact tcgatgcctt
     1981 ttgtttcctt catcacgcgc gcgatcaagc cttctgtaat atcttggatt tcttgctcag
     2041 taaggaagga cgtttccaag tcgacctgag taaattcagg ctggcggtct ccacgcaagt
     2101 cttcgtcacg gaaacattta acgatttggt agtaacggtc aaaaccagca ttcatcaaga
     2161 gctgtttcgt gatttgtgga ctttgaggaa gagcgtaaaa atgcccctta ttaacacgag
     2221 acggcactaa ataatcacgc gccccttcag gcgttgactt agaaaggaat ggtgtctcca
     2281 cgtcgataaa ctccaactca tccaagtagt tgcggataga gtgggtcacc ttggcacgaa
     2341 gtttaagatt ttccaacatt tctggacgac gaaggtcaag gtaacggtaa cgcaaacgtg
     2401 tatcgtcatt tgcctcaatg ccatccttaa tctcaaatgg tgttgtctta gctgtgttaa
     2461 gcacaataag agctgtcacg tttaactcaa ccgcaccagt tggcaactta tcattggctt
     2521 gctcacgcgc agcgacctga ccagtcacct caataacaaa ttcgctacga aggctttcag
     2581 ctgttgccat aacctctgca gatacttttt cagggttgat aaccaactgc atgattcctt
     2641 cacggtcacg aagatcgata aagatcaaac caccaaggtc acgacgacgg ccaacccatc
     2701 ctttcaaggt tatttcttgt ccgatgtgtt cctcacgaac acgaccagca tacatactac
     2761 gtttcattat ttctctcctc ttttattctg ttactatttt accataaaag cgcaggctct
     2821 tcatgaaaat catcagaaaa gtttgccagt ctttaaaagt caggtgaaag ccctaaaaat
     2881 tagcgctaat act
//
LOCUS       DAVLVZ010000110         2872 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000110 DAVLVZ010000000
VERSION     DAVLVZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.110"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..873
                     /locus_tag="VYN77_001947"
     CDS             <1..873
                     /locus_tag="VYN77_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1559349.1"
                     /translation="KIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKK
                     FDIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKG
                     QILIDGKDIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLA
                     NCTDFIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNN
                     EKKIQESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYK
                     NLIEKTKMAEEFIY"
     gene            884..1468
                     /locus_tag="VYN77_001948"
     CDS             884..1468
                     /locus_tag="VYN77_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1559350.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLKKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1468..2163
                     /locus_tag="VYN77_001949"
     CDS             1468..2163
                     /locus_tag="VYN77_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1559351.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            2151..>2872
                     /locus_tag="VYN77_001950"
     CDS             2151..>2872
                     /locus_tag="VYN77_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559352.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLR"
BASE COUNT         1045 a          364 c          549 g          914 t
ORIGIN      
        1 aagataaaag actctttaga tgcatctaaa gagggcttga tggaaatatt ttatttatcg
       61 cccaaaatag aaagattaaa agaaattcaa aatcaagatt tacaagaagg cgatgactat
      121 agcttaaaaa aatttgatat tgatctaaaa gatgttgagt ttgcctacaa taaagacgca
      181 aaagttttaa atggtgtaag ttttaaagct aagcagggag aggtcactgc tttggtaggt
      241 gcaagtggct gcggtaaaac aactatcttg aaacttatat caagacttta tgattatgac
      301 aagggacaaa tcttaatcga tggcaaagat ataaaagaaa tatcaacaga atctcttttt
      361 gataaggtct ctatagtttt ccaagatgtg gttctcttta atcaaagcat tatggaaaat
      421 attagaatcg gaaagcaaga tgcaagtgac gaagaggtta aaagagcagc aaaacttgca
      481 aattgcacag attttataga aaaaatggat aaaggtttcg atacagttat tggtgagaac
      541 ggagctgagc tatcaggagg agaaagacaa agattatcaa tagccagagc cttcttaaaa
      601 gatgcgccga tattgatctt agatgagata acagcaagcc ttgatgttaa caacgagaaa
      661 aagattcaag agtctttaaa taatttagtt aaagataaaa ctgttgtaat catttcacat
      721 agaatgaaat ccatagaaaa tgcagacaag atagtagttc ttcaaaacgg aagagtagaa
      781 agcgaaggta agcatgaaga gcttttacaa aaatcaaaaa tttacaaaaa tttaatagaa
      841 aagacaaaaa tggcagaaga atttatttat taggaggact acaatggata ataaaaaatt
      901 aaaagtaaaa gatttagtaa gtatcggtgt ttttggcgta atttattttg ccttcatgtt
      961 tggagttggt atgatgggct tgattccaat attgttctta atatacccga cagtattagc
     1021 catagttgca ggaactgttg ttatgttatt tatggctaag gttcaaaagc catgggcact
     1081 atttatattt ggtatgatat caccacttgt gatgtttgca gctggtcata cctacgtagt
     1141 tgtggtttta tcacttatag taatgataat agcagaatta attagaaaga ttggtaatta
     1201 taattcattt aaatacaata tgctttctta tgcaatcttc agcacatgga tatgtagctc
     1261 tttaatgcaa atgcttttag caaaagaaaa atatatggag tggtctttga tgacgatggg
     1321 aaaagattat gttgatgtat taaaaaagtt aataacttat cctcacatgg ctttagtagc
     1381 cttaggtgct ttcttaggag gaattcttgg agcatatata ggcaaggctc tattgaaaaa
     1441 acactttgaa aaagcaggca ttgtataatg cctacttttt cttcaaatgc tccctttaat
     1501 cccaatccga tctctaagat attagtcgta tttatcacag gcttaactgt tatgcatagc
     1561 ataaacatcc gttttgagct ggcaattgtt tgtcttattg gtattttgct ttatttgaat
     1621 ggatacaaaa aaacactttt caaatggatt atattgtgtg ggatacttta ctccttgcct
     1681 aattttatgg tgttatctga attaaaccct atagttaaga tgtttttgag tatacctatt
     1741 gttattagaa tgtttatttt accatttatg gcagcaagct ttatgataaa gacctcggat
     1801 gtaggcgcaa taatttcatc gatggataag cttaagattt caaagaatgt atccatacct
     1861 attgcggtta tgtttagatt cttcccatct tttaaggagg agaagaaaaa catcaaaatg
     1921 gctatgagag taagagggat aagttttaaa aacccagtca aatatcttga atatgtttct
     1981 atgccactac tcattatatc atctaatata tcagatgaca ttgcaaaagc ggcagaaaca
     2041 aaggcaatag aaaatccaat tgccaagacc agatacattc gcgtaaagat acagctaatt
     2101 gattttgttt atgttttagc ggttgctgga cttattgtgg gaggcttaat atggttgaaa
     2161 taaaaaattt aagtcttgat tatggtgaag agcatatatt agatgatata tcactatcca
     2221 tagccgaggg agagtgcgtg ctatttacag gaaaaagtgg aaatggtaag tcatctttaa
     2281 taaattcaat caatggacta gctgtaaggt atgataacgc aaagacaaag ggcgaaataa
     2341 ttattgatgg taagaatata aaaaatttgg aactttatca aatctcaatg cttgtttcaa
     2401 ctgtttttca aaatcctaag acatattttt ttaatgtcaa tacgacatta gaattattat
     2461 tttatttgga aaatatcggt cttgcaagag aagagatgga caggcgtttg aaggatatgc
     2521 ttaagatatt cccgataaaa aatcttttga acagaaatat atttaatcta tccggcggtg
     2581 aaaaacaaat tctttgcatt gcagcttctt atatagcagg tacaaagatt atagttatgg
     2641 atgagccttc atcgaattta gatattaaaa gcataagtgt tttggcaaag atgctaaaga
     2701 tattaaaaga gaaaggcata agcataattg ttgcagagca tagaatttat tatttgatgg
     2761 acatagttga ccgtgtattt ttaatagata aaggaaagct taaaaaaact tatactagaa
     2821 gtgaattttt aaagctagat aaaaatgaat taaatgcttt aagtttaaga ga
//
LOCUS       DAVLVZ010000111         2864 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000111 DAVLVZ010000000
VERSION     DAVLVZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.111"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            134..1426
                     /locus_tag="VYN77_001951"
     CDS             134..1426
                     /locus_tag="VYN77_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1559353.1"
                     /translation="MLSQYVGRKTSCIENEITKTRNHSRQRGNIMRVVFGIDVSKASS
                     EVAILVNGDKVHGYTIPNDSIGFNRLLGDLKTVSNPEIVFEATGVYSRRLQAFLEDNS
                     YAYTRLNPLAAKKQLDSLRVRKTDKIDAEKLAQSQFVLNRQPTYVQEEVYQELRDLSR
                     FYQNLTEDIVRAKNRLHKVLQVTFPELESLLSSPTGEQYWNLVIAFPCKEMVLELSRD
                     DLSTVIRNSTSKRISDKRVTYLTEKLTDLAHKSYCAVKKTSPMLEEVRYYAQELLRLS
                     ERRQVVLDEMVSLAQPLPEYEILLSIPGIAETTVTSLIGELGDIRRFQSANQINAFIG
                     IDLRHYESGNFLAKEHITKRGNPYARKILFKCIHNIASASHTNPCHIADFYEKRKRQS
                     QTTSTKPHTIASIHRLIRTMYYLITHNKIYDYNLATSQ"
     gene            complement(1819..2211)
                     /gene="rpsI"
                     /locus_tag="VYN77_001952"
     CDS             complement(1819..2211)
                     /gene="rpsI"
                     /locus_tag="VYN77_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1559354.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(2231..2677)
                     /gene="rplM"
                     /locus_tag="VYN77_001953"
     CDS             complement(2231..2677)
                     /gene="rplM"
                     /locus_tag="VYN77_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1559355.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
BASE COUNT          833 a          586 c          574 g          871 t
ORIGIN      
        1 gaggaaatct ccgacgggag aaagtactca ctactttttc tttgtgataa agtagaggtg
       61 tcttgttaag tcgagggttc ttttgacgct agacgtcgta cataaaactg gcaagacgcc
      121 tgttttaggt ggaattttat cacagtatgt gggacgcaaa acgtcgtgta ttgaaaatga
      181 aatcactaaa acaaggaatc attcaagaca aagaggtaat atcatgcgag tcgtatttgg
      241 gattgatgtc agtaaggcaa gttcagaagt ggctatttta gtcaatggtg ataaggttca
      301 tggctacacc atacctaatg attctatcgg cttcaatcgg ctacttggtg acttgaaaac
      361 tgtctctaat cctgagattg tcttcgaagc aacaggtgtc tattctcgtc gtcttcaagc
      421 ctttcttgaa gataatagct acgcttatac acggttaaat cctctagctg ctaaaaagca
      481 acttgacagc ttacgtgttc gtaaaacaga caaaatcgat gccgagaaat tagcacaatc
      541 tcagtttgtt ctgaatcgtc aaccaactta cgtccaagag gaagtctatc aagagttacg
      601 tgacctaagc cgcttctatc aaaatctgac tgaagatatt gttcgagcta agaaccgtct
      661 ccacaaggtc ttacaagtca cgtttccaga gttggaaagc ctcttgtcct caccaactgg
      721 cgagcaatac tggaatttag tcatagcttt tccttgtaag gaaatggtgc tagagctgag
      781 cagggatgat ttatcaactg tcattcgaaa ctcgacttca aaacgtatct ctgacaagcg
      841 ggtaacttat ttgactgaga agcttactga tctagctcac aaatcttatt gtgctgtcaa
      901 gaaaacctct ccaatgcttg aagaggtccg ttactatgcc caagaattgc ttcgactttc
      961 tgaacgcaga caagttgttc tagacgaaat ggtttcctta gctcagcctt tgccagaata
     1021 tgagattctt ctctctattc ctggtatcgc agaaacaacg gtgacatcac tcattggcga
     1081 attgggtgat attcgtcggt ttcagtctgc caatcaaatc aatgccttta tcggcatcga
     1141 cctgcgtcac tatgagtctg ggaacttcct tgccaaggaa cacatcacca aacgagggaa
     1201 tccttatgcc agaaagattc ttttcaagtg tattcacaac atcgcttcag ctagtcatac
     1261 caatccttgt catatcgcag acttttatga gaaacgaaaa agacaatcgc aaacgacttc
     1321 caccaagcca catacgattg cctctataca tcgtcttata aggacaatgt attacctcat
     1381 tacgcataat aagatttacg actataactt agccacaagt caataaactt atctatgcca
     1441 cattattcta acaccttgtt cttaaaaaag ctagataagg tgttcctttt gtgtgcattt
     1501 ttatcgctac ttagcacaga aaaagaggac aataactgat agttagctac tgcccttaat
     1561 ttattttttc tttaaatcct ggagacgctt gacaaatggt agaaaaaaag cacccatgag
     1621 gtaaactctt gagtgctttg aaacgttgat ataattgtat tgattaacgt tttgaggcaa
     1681 ctgaccttgt caggttgcag ccacatttgt aagcgactaa agtcgcaaca actgtgtcaa
     1741 ttgcaccaat ttagtaagaa agtataaaaa aagaacaccc cgaaaggtgc tctgtataag
     1801 tatagtaatt ctttcgaatt aacgtttact aaattgtgat gctttacgag ctttcttaag
     1861 acctggtttc ttacgttcaa ctttacgtga gtcacgtgta agaagtcctg cgcgtttcaa
     1921 tgaatcgcgg aagtctgggt ctacttgaag aagggcacga gcgataccgt gacggatagc
     1981 tcctgattga ccagcgtatc caccacctac aacgttaacg aaaacgtcgt atgaacctac
     2041 agttgaagta actgcgaatg gttggttgat gacaagacga aggtcagcgt gtgggatgta
     2101 ctcttcaaca tcttttttgt taacagtgat tttaccagtt cctggaacaa ggcgaacgcg
     2161 tgcaacagcg tttttacgac gtccagtacc tgcatattgt gcttgtgaca tactttattt
     2221 gttcctttcc ttagataagt cctgaaatgt caagaacttc tggttgttgt gcagcatgag
     2281 tgtgctcagc tccaacaaat actttcaact tcataccttg agcgcgtcca agtgtattgt
     2341 gtggaagcat acctttaact gatttctcaa tcaaacgtac tgcattttta gaacgaagtt
     2401 cacctgcaga gatttgtttc aatccacctg ggtggtttga gtgagtgtag tagattttat
     2461 cagttgcttt tttaccagtc aatttaactt tttcagcatt gataacaatc acgaagtcac
     2521 ctgtatcagt gtgtggtgta aatgttggtt tgttttttcc gcgaagtacg ctagcaacta
     2581 ctgcagaaag acgtccaagt ggtacatcag ttgcgtcaac tacgtaccat ttacgttcaa
     2641 cttggcctgg tttagccata aatgttgttt tgttcatgat ttctcctata tatagttcga
     2701 tttttgttta cggtgatggg tgttccttcc catcgaaaag tatttggaag gttccggggc
     2761 ctttcaaatg gggtaaacaa taccgcctac tatcatacca aatttcaccc ctaaaagtca
     2821 atagatatga aaaatatttt tcggtaactt ctcaaagtaa ctga
//
LOCUS       DAVLVZ010000112         2719 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000112 DAVLVZ010000000
VERSION     DAVLVZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.112"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            112..1092
                     /locus_tag="VYN77_001954"
     CDS             112..1092
                     /locus_tag="VYN77_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1559356.1"
                     /translation="MKVSVIIPVYNVEKYLRRCLDSVVNQTYKDIEVILVNDGSPDNS
                     KEICEEYVAKYSNIQLINQKNAGLGAARNTGLQYITGNAVTFVDSDDWLELDAIEYYV
                     ASMKKSDADIVVTQMIRKKEYFSNEGTNGTTIKEEVLNQEQFAKKYFKIDGNNIEYYA
                     CAKLYKREIAREVKYPVGLFAEDVPAAFGYIIRSQKIFYSTKVTYNYFFNDNSLTAKF
                     TDKDFDLEKIWDLVVEEAKVYGNEDYILYAKVNRYRIDFNLLCRIALSENKSDIEKYS
                     QEIVVLLGKVKENKKILLKYLPFSRKVIFRLFIVDYTLGRNVLRMFKNIV"
     gene            1122..2186
                     /locus_tag="VYN77_001955"
     CDS             1122..2186
                     /locus_tag="VYN77_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1559357.1"
                     /translation="MYYFIKVEKDLKNTAGIKAPDDIEKISEELGMKEIRFPKFPFEK
                     NKVIQKLWLFCVVGYNWISLLWRLKKNDVVIYQHPMYGVRVANFAIPLLKKYKNIKFI
                     SVIHDLESLRKGIQGVIEDNETTNAIADKELLSKFDKVISHNPKMTEYLEGIGIKKEN
                     LVELQIFDYLDPSEIEEKIEDGVVIAGNLAKGKSSYIYKLLENELNFKLNLFGPNFIN
                     EELPENVEYFGSLPPNKLPQKLVGKFGLVWDGDSLETCSGNTGNYLKYNNPHKTSLYL
                     ASGIPVIIWKEAALAQFIEENNVGITVNNLSEIEFVMQNISEGEYLSIKRNTMQLGEK
                     LRNGYFYRQAISKCKNDFMK"
     gene            2351..>2719
                     /locus_tag="VYN77_001956"
     CDS             2351..>2719
                     /locus_tag="VYN77_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEV1559358.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLLRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRP"
BASE COUNT         1044 a          305 c          536 g          834 t
ORIGIN      
        1 tattttaaaa atatggaaga aatgatagag aaccataaga ttagatttgg aagttttgaa
       61 cacggaaatg caactcaaaa aatagtggaa ttattagaag aaggtaatta aatgaaagta
      121 agtgtaataa tacctgttta taatgttgaa aaatatttaa gaaggtgttt ggattccgtt
      181 gttaatcaaa catacaaaga tattgaagtg attttggtaa atgatggttc tccagataat
      241 tcaaaagaaa tatgtgaaga atacgttgct aaatattcca atattcaatt aataaatcaa
      301 aagaatgcag ggctaggtgc agcaagaaat actggtctac agtatataac aggaaatgcg
      361 gtaacttttg tcgattctga tgattggtta gagttagatg caattgaata ttatgttgca
      421 tctatgaaaa agagtgacgc agatattgta gttactcaaa tgattagaaa aaaagaatat
      481 ttttctaatg aaggaactaa tgggacgact ataaaagaag aggtcctaaa tcaggaacag
      541 tttgcaaaaa aatattttaa aatagatggt aataatatag agtattatgc ttgtgcaaaa
      601 ctttataaga gagagattgc tagagaggtt aaataccctg ttggtttatt tgcagaagac
      661 gtccctgcag catttggtta tattatacgt tcacaaaaaa ttttttatag tacaaaagta
      721 acgtataatt atttctttaa tgataatagt ttaacggcta aatttacaga taaagatttt
      781 gatttggaaa agatatggga tctagtcgta gaagaagcaa aggtttatgg gaacgaggat
      841 tatattttat atgccaaggt gaatagatat agaatcgatt ttaatctttt gtgcagaatt
      901 gctttatcgg aaaataaatc tgatatagaa aaatatagcc aagaaatagt ggttctactt
      961 ggaaaagtta aagaaaataa aaaaatttta ttaaagtatt taccgttttc taggaaggtg
     1021 atatttagat tgtttatagt tgattatacg ctaggaagaa atgttttgag aatgtttaag
     1081 aatattgtgt agttaacaaa tattggtata aggtgataga aatgtattat tttataaaag
     1141 tagagaaaga tttaaaaaat acagccggga ttaaagcgcc tgatgatata gaaaaaattt
     1201 cagaagaatt gggaatgaaa gaaatacgct ttcccaagtt tccttttgaa aaaaataaag
     1261 ttatacaaaa actctggctt ttttgtgttg taggttataa ttggataagt ctgttgtgga
     1321 gacttaaaaa gaatgatgta gttatttacc aacatccaat gtacggagtg cgtgttgcaa
     1381 attttgcaat tcctttattg aaaaagtata aaaatataaa atttatttca gtaatacatg
     1441 atttagaatc gttaagaaaa ggaatccaag gagtcattga agataatgaa acaacaaatg
     1501 ctattgcaga caaagagttg ttgtcaaaat ttgataaagt gatttcccat aatccgaaaa
     1561 tgacagaata tttagaaggg atagggataa aaaaagaaaa tttagttgaa ttgcaaatat
     1621 tcgactacct agatccatca gaaatagaag aaaagattga agatggagtg gttatagcgg
     1681 ggaatctagc aaaaggaaaa agttcatata tatataagtt gttagaaaat gaactgaact
     1741 tcaaattaaa tctttttggg ccaaatttta ttaacgaaga gctaccagaa aacgttgagt
     1801 attttggtag tttacctcca aataaattgc ctcaaaagct agtaggtaag tttggtttgg
     1861 tttgggacgg tgatagtcta gaaacttgta gtggaaatac tggtaactat ttgaagtata
     1921 ataatccaca taaaacctca ttatatcttg cttcaggaat acctgtaatt atctggaaag
     1981 aagctgcgtt agcacagttt attgaagaaa ataatgttgg tattactgta aataatttgt
     2041 cagaaattga gtttgtgatg caaaatatat ctgagggcga atatttatca ataaaaagaa
     2101 atacaatgca attgggtgaa aaattaagaa atggatattt ttatcgtcag gcgatatcta
     2161 agtgcaaaaa tgatttcatg aagtaagagg gcatataaaa agacggaaaa tatttagcaa
     2221 taaatactgt agcttatttt ttattgaagt ggatgtgtaa tttcagataa ctatggagtt
     2281 gctcaccaat taaatcagaa ctcaaaaaac aatagactcc ctgcgaaaca aaatatggta
     2341 tagtagttct atgaatgatg aagcaagtaa acaactaact gatgcacgat ttaagcgtct
     2401 tgttggtgtt cagcgcacga cttttgaaga gatgttagct gtattaaaaa cagcttatca
     2461 acttaaacac gcaaaaggtg gacgaaaacc taaattaagc ctagaagacc ttcttatggc
     2521 cactcttcaa tatgtgcgag aatatcgaac ttatgaagaa attgcggctg attttggtat
     2581 ccacgaaagc aacttactcc gtcggagcca atgggttgaa gtaactcttg ttcaaagtgg
     2641 tgttacgatt tcaagaactc ctctcagttc tgaggacacg gtaatgattg atgcgacgga
     2701 agtacaaatc aatcgccct
//
LOCUS       DAVLVZ010000113         2632 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000113 DAVLVZ010000000
VERSION     DAVLVZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.113"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            194..670
                     /locus_tag="VYN77_001957"
     CDS             194..670
                     /locus_tag="VYN77_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1559359.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAETTA"
     gene            708..1013
                     /locus_tag="VYN77_001958"
     CDS             708..1013
                     /locus_tag="VYN77_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1559360.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            1061..2536
                     /locus_tag="VYN77_001959"
     CDS             1061..2536
                     /locus_tag="VYN77_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1559361.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          806 a          492 c          515 g          819 t
ORIGIN      
        1 ctctttgatt ttcattgagt attagaacag aaaaaatgct tggagtattt gtttgtgtgt
       61 ttatttttat ataacaaact ataaacaaaa aaaaatataa aaaaagagac aaaaaagaac
      121 agaaagtaat tgacaacggt ttcatatagt gttatactta tggcataaag ttaatggaaa
      181 gaagggaaaa cttatggtat tagattattt ctttgacaaa aaccttgtgt tttgcttaga
      241 agcggataat caagaacaac tctttgatca ggttgcaact ttattggaag aacgagaaat
      301 tgtcactcca acttatcgtg aagccttgat cacgcgtgaa aagtcatttc caactggttt
      361 agatatggaa tttctaggaa aggacttgcc aaatgtagcg attcctcata cagatattgt
      421 tcataatcta gctgagaaag tggtggttgt tcgattagaa aaaccagtaa cttttcacaa
      481 tatgatagct cctgataagg aagtagaagt atccttgctc ttctttatca tcaataactc
      541 aagttcaagt caaacaaata ttctggctca gttgatggac tttttcacag gaaatggaca
      601 tcttgaagac ctatcaaaaa tttccgaacc agaaaaactt tatgcttaca ttgctgaaac
      661 aaccgcttaa tcttgtctat taaaaaataa aatcggagga aatctaaatg attaaaattc
      721 ttgctgcctg cggtgcaggt gttaactcaa gtcaccaaat taaaagtgct ctagaagaag
      781 aactttcaaa ccgtggttat gatgttcact gtgatgcagt catggtgaaa gatgtaaacg
      841 aagaccttat gaaaggttac gatatcttta caccaatcgc tgcaacagac cttggttttg
      901 aaccaggtat cccagttatc gaagctggac caatcttatt ccgtatccca gcaatgagcg
      961 ctccagtatt tgacaatatt gaagcagcta ttaaagaaca tggattaagc taattctaat
     1021 ttgttaacat tcatataaca taaaaaaggg aggaactgtt atggatgcaa tctttgacct
     1081 aatcggaaag gttttcaatc ccatcttaga aatgggtgga cctgtcatca tgttgatcat
     1141 tttgacagta ttggctttac tttttggagt gaaattctcc aaagcgcttg aaggtggtat
     1201 caaacttgcc atcgctctta caggtatcgg tgctatcatc ggtatgctaa atggtgcttt
     1261 ctcagcatca cttgcaaaat tcgttgaaaa cactggtatc caattgagta ttacagacgt
     1321 tggttgggca ccactcgcaa caatcacttg gggttctgct tggacactat acttcttgct
     1381 catcatgttg attgtcaacg tagtgatgct tgctatgaag aaaaccgata cacttgatgt
     1441 cgatatcttt gatatctggc acttgtctat cacaggtctc ttgattaaat ggtatgctga
     1501 taacaatggt gtgagtcaag gggtttcact ctttattgct acagcagcta tcgtccttgt
     1561 cggtgtgttg aaaattatca actctgactt gatgaaacct acatttgatg accttcttaa
     1621 cgccccaagt tcatcaccaa tgacatcaac tcacatgaac tacatgatga acccagttat
     1681 catggttttg gataagattt ttgaaaaatt cttcccaggc cttgataaat atgactttga
     1741 tgctgctaaa ttgaacaaga aaatcggttt ctggggatct aaattcttca tcggtttcat
     1801 ccttggtatc gttatcggta ttatgggaac tccacatcca attgcaggtg ttgcagatgc
     1861 agataaatgg cgtcttgtta tcaaaggatg gttgtctctt ggtttgactg ccggtgtatc
     1921 tttggaactc ttctcactta tcggttcatg gttcatcgca gccgtagaac cactatcaca
     1981 aggtattaca aacgttgcta ctaaacgtct tcaaggacgt aaattcaata tcggtcttga
     2041 ctggccattc atcgctggtc gtgctgaaat ctgggcttgt gccaacgtac ttgcaccaat
     2101 catgttgatt gaagcagtgc ttctttcaaa agttggaaat ggtatcttgc cacttgcagg
     2161 tatcatcgct atgggtgtta ctccagctct cttggttgta actcgtggta aattgctccg
     2221 tatgattatc ttcggaacac tcttgttgcc actcttcctt ctttcaggta cacttattgc
     2281 accatttgca acagaacttg ctaaaggtgt aggtgccttc ccagaaggtg tgagccaaac
     2341 tcaattgatt actcactcta ctcttgaagg accaatcgaa aaacttcttg gttggacaat
     2401 tggtaacact acaactggtg atatcaaagc aatccttggt gcagtagtct tccttgtatt
     2461 ctatatcggt atctttgctt ggtacagaaa acaaatgatc aaacgtaacg aagagtacgc
     2521 agcaaaagca aaataatgcg ctcctataaa atataaactt aatgaaaatc aaagagcaaa
     2581 ctaggaagct agccgcaggt tgctcaaaac acagttttga ggttgtagat aa
//
LOCUS       DAVLVZ010000114         2589 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000114 DAVLVZ010000000
VERSION     DAVLVZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.114"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     misc_feature    66..201
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            240..740
                     /locus_tag="VYN77_001960"
     CDS             240..740
                     /locus_tag="VYN77_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1559362.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEALASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            816..1184
                     /gene="rplL"
                     /locus_tag="VYN77_001961"
     CDS             816..1184
                     /gene="rplL"
                     /locus_tag="VYN77_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1559363.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            1431..>2589
                     /locus_tag="VYN77_001962"
     CDS             1431..>2589
                     /locus_tag="VYN77_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1559364.1"
                     /translation="MSLRNKIEQHIKELEGGKFQKLGDAYLSRKYNFNIVSLGSQEGT
                     DKTTKGIPDSYAVENGKYVYIMYGTHKSVISKLEGDIQSVKKKILEENIAEDKVGRLI
                     CCHTSSNITIKQKEDLEKMAEPYHLELIGINEIANDLTKIDFQYLAKEYLSISESTEQ
                     VWSINDFIRIHDESKTNAPISNDYIGDVSEIINTIKSSEKRIFLISAKPGTGKTRLAI
                     EICSLLDRNKYNIICVKSNNQDIYQDVKRNLNLHKENIVFIDDVNTTQNYISTLGLLN
                     TTSNIRFILTVRDYAKKDVINNIKVYGYNNIEPELIKDDNFKELLNQFSRNDFTNQEI
                     EHIKTISKSNPRIAVIAAKLSSSQDLTNFNDEIDILKDYYEEILNKNNIIYA"
BASE COUNT          930 a          385 c          487 g          787 t
ORIGIN      
        1 acgaaaaatt ttaaaaaaag tttgcaaaaa tcttgcattc tttttttgac tatgctatac
       61 ttatatacgg tttaaaaaaa ctgcctaaga cagtagggga gctcgactca taaatatcct
      121 accgaggaca aaacgtatca tgtaaaaaga agcgtattgt actttcgtgt ctaggtttgg
      181 gcgcgttttt ctttttgaaa aattccccaa gcaaaataat tacggaggtg aacacactaa
      241 tgagtgaagc aattattgct aaaaaagcgg aactagttga cgtagtagct gaaaaaatga
      301 aagctgctgc atctatcgtc gttgtagacg ctcgtggttt gacagttgag caagatacag
      361 ttcttcgtcg tgagcttcgt ggaagcgaag ttgagtataa agttattaaa aactcaatct
      421 tgcgtcgtgc agctgaaaaa gctggtcttg aagctcttgc atctgtattt gttggaccat
      481 ctgcagtagc attttctaat gaagatgtta tcgcaccagc gaaaatcttg aacgactttt
      541 ctaaaaacgc tgaagcactt gaaattaaag gtggtgcaat cgaaggcgct gtcgcatcta
      601 aagaagagat tcttgcactt gcaactcttc caaaccgcga aggacttctt tctatgctcc
      661 tttctgtact tcaagcgcca gtgcgcaacg ttgctcttgc agtcaaagcg gttgcagaaa
      721 gcaaagaaga cgcggcttaa tcttaagcta cacagcgtag cctagctacg aaaaaaacta
      781 ttataaaatt taaaacttat ttggaggaaa taacaatggc attgaacatt gaaaacatta
      841 ttgctgaaat taaagaagct tcaatccttg aattgaacga ccttgtaaaa gctatcgaag
      901 aagaatttgg tgtaactgca gctgctcctg tagctgttgc tgcagctgat gcagctgatg
      961 ctggtgctgc taaagattca ttcgacgttg aattgacatc tgcaggcgac aaaaaagttg
     1021 gcgttatcaa agttgtacgt gaaatcactg gtcttggtct taaagaagct aaagaacttg
     1081 ttgacggtgc accagcactt gttaaagaag gcgttgcaac tgcagaagct gaagaaatca
     1141 aagctaaatt ggaagaagct ggagcttcag ttactcttaa ataatagagc aactacatta
     1201 gtagcttaaa aacatgatta aaccgctatt cttaggagta gcggtttttc tttttgttta
     1261 aaaaggggca aactttttat atagttgttt aggaaataaa taatttttgt ataattggtt
     1321 tactactcta aattaacgta gtacaagagt ttgttaatta aactatacct tatggttttg
     1381 actagtgtga tataattatg ttgaaatact tatctggaaa ggtttttata atgagtttaa
     1441 gaaataaaat tgagcaacat attaaagagc tagagggagg gaagtttcaa aaactaggtg
     1501 acgcgtacct aagtagaaaa tacaatttta atattgtttc attaggctca caggaaggaa
     1561 cagataaaac aactaaaggg atcccagatt cttacgctgt cgaaaatggt aaatatgttt
     1621 atattatgta tggcacccat aagtctgtaa tatctaaatt agaaggagac attcagtcag
     1681 ttaagaaaaa aattttagag gaaaatattg ctgaagataa agttgggaga cttatttgtt
     1741 gtcatacttc ttcaaatata acaattaaac aaaaagaaga tctagaaaaa atggctgagc
     1801 cttatcatct agaattgatt ggaattaatg aaattgctaa tgatcttaca aagatagatt
     1861 ttcaatattt agcaaaagag tatctatcaa tcagtgaatc tacagagcaa gtttggtcta
     1921 tcaatgactt cataagaatt catgatgaga gtaaaactaa tgctcctata tcaaatgact
     1981 atattggaga tgtaagtgaa attataaata ccattaaatc aagtgaaaaa cgaatcttct
     2041 taatatctgc taagcctgga acagggaaaa ctagattagc aattgaaatt tgctctttgc
     2101 tggatcgaaa taaatataac attatttgtg tcaagagcaa taatcaggat atttaccaag
     2161 atgtaaaacg caatctaaat ttacacaaag aaaatattgt ttttattgat gatgttaata
     2221 ctacacaaaa ttatatttct acacttgggc tactgaacac gactagtaat attcggttta
     2281 ttcttacggt acgtgattat gctaaaaaag atgtaatcaa taatattaaa gtttatgggt
     2341 acaataatat tgagccagaa cttataaaag atgataattt taaggaatta ctcaatcaat
     2401 tttctcgaaa cgattttacc aatcaagaaa ttgaacatat aaaaacgata tctaaaagca
     2461 atccgcgtat tgcagtcatt gcagcaaagt tatctagctc tcaggatttg actaatttta
     2521 atgatgagat tgatatttta aaagattact atgaagaaat tttaaataaa aacaacatta
     2581 tttatgcag
//
LOCUS       DAVLVZ010000115         2490 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000115 DAVLVZ010000000
VERSION     DAVLVZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.115"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            47..1351
                     /locus_tag="VYN77_001963"
     CDS             47..1351
                     /locus_tag="VYN77_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1559365.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            1364..2170
                     /gene="yidA"
                     /locus_tag="VYN77_001964"
     CDS             1364..2170
                     /gene="yidA"
                     /locus_tag="VYN77_001964"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1559366.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
BASE COUNT          782 a          597 c          454 g          657 t
ORIGIN      
        1 tcctactatt ttaccaaaaa gagcaggatt ttgctataat ggtcatatga acgaaaaagt
       61 attccgtgac cctgttcaca actacatcca tgtcaataat caaatcatct atgacttgat
      121 taatacaaaa gaatttcagc gtttgcgccg gatcaaacaa ctgggaactt ccagttatac
      181 cttccacggt ggagaacaca gtcgcttctc tcactgtcta ggagtctatg aaattgcacg
      241 acgcatcaca gagattttcg aagaaaaata tcctgaggaa tggaatcctg ccgagtctct
      301 cttgaccatg accgctgctc tcctacacga ccttgggcat ggtgcctact cccatacttt
      361 tgaaaatctc tttgatacag accatgaagc cattactcag gagattattc aaaatcctga
      421 gacagagatt caccaagtcc tgctacaagt ggcacctgat ttcccagaaa aggtggccag
      481 tgtcattgac catacctatc ctaataagca ggtcgtgcag ctcatttcta gtcagattga
      541 cgcagatcgc atggactatc tcttgcgcga ctcctatttt acaggagcat cctatgggga
      601 atttgacctg actcgaatcc tccgagtcat tcgtcctatc gaaaatggta tcgcctttca
      661 gcgcaatggc atgcacgcca tcgaagacta cgtcctcagt cgctaccaga tgtacatgca
      721 ggtttatttc caccccgcaa cacgcgccat ggaagttctc ctacagaatc ttctcaaacg
      781 cgccaaggaa ctctatcctg aggacaagga tttctttgcc cgaacttctc cacacctcct
      841 gcctttcttc gaaaaaaatg tgaccttgac tgactatctg gctctggatg atggcgtgat
      901 gaatacctac ttccagcttt ggatgaccag tcctgacaag attcttgcag atttatcgca
      961 tcgctttgtc aaccgcaagg tctttaaatc cattaccttt tcacaagaag accaagacca
     1021 actcgctagc atgagaaaat tggttgagga catcggcttt gatcccgact actatactgc
     1081 cattcataag aactttgacc tcccttatga tatttaccgt cccgaatctg aaaatccaag
     1141 gacacagatt gagattttac aaaaaaatgg agaactggca gaactctcta gcctgtcccc
     1201 tatcgtccaa tcccttgctg gaagtcgcca cggagataat cgcttttatt tcccgaaaga
     1261 aatgttagac caaaacagca tcttcgcaag catcacccag caatttttac acttgattga
     1321 gaacgatcat tttaccccaa ataaaaacta aaagaggaaa tttatgagta ttaaactaat
     1381 cgccgtcgat attgacggaa ccctagtcaa cagccaaaag gaaatcactc ctgaagtctt
     1441 ttctgccatc caagatgcca aagaagctgg tgtcaaagtc gtgattgcaa ctggccgccc
     1501 catcgcaggt gttgccaaac ttctggacga cttgcagttg agagaccaag gtgactatgt
     1561 ggtgaccttc aatggtgccc ttgtccaaga aactgctact ggccatgaga ttatcagcga
     1621 atccttgacc tatgaggatt atctggatat ggaattcctc agtcgcaagc tcggtgtcca
     1681 catgcatgcc attaccaagg acggtatcta tactgcaaat cgcaatatcg gaaaatacac
     1741 tgtacacgaa tcaaccctcg tcagcatgcc tatcttctac cgtacccctg aagaaatggc
     1801 tggcaaggaa attgtcaagt gtatgttcat cgatgaacca gaaattctcg atgctgcgat
     1861 tgaaaaaata ccagccgaat tttacgagca ctactctatc aacaaatctg ctcctttcta
     1921 cctcgaactc cttaaaaaga atgtagacaa gggttcagct attactcact tggctgaaaa
     1981 actcggattg accaaagatg aaaccatggc aatcggtgac gaagaaaatg accgtgccat
     2041 gctggaagtc gttggaaacc ccgttgtcat ggaaaatgga aatccagaaa tcaaaaaaat
     2101 cgccaaatac atcactaaaa caaatgacga atccggcgtt gcccacgcca tccgaacatg
     2161 ggtactgtaa aagtatcatt tttcaataag aattgattag caataaaatc caatgaattt
     2221 ttttagcaaa ctatttaatt taaaacaaaa taatcataat agagacacaa attctgattg
     2281 taacaatttt tacctaaacg atttagaatg tggccttact cctgggcaac tcatactcat
     2341 agattggact caaaaaacag ggagaaatta taatttccca agatatttta aatactctct
     2401 tcaaattgac cctgaatcta cacacaatca attatacaaa ttaggatact tcactaaaaa
     2461 taagacttta tcatatctta cagtagtaga
//
LOCUS       DAVLVZ010000116         2431 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000116 DAVLVZ010000000
VERSION     DAVLVZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.116"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..801)
                     /locus_tag="VYN77_001965"
     CDS             complement(<1..801)
                     /locus_tag="VYN77_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEV1559367.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLGLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETF"
     gene            complement(788..1558)
                     /locus_tag="VYN77_001966"
     CDS             complement(788..1558)
                     /locus_tag="VYN77_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV1559368.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(1584..2423)
                     /locus_tag="VYN77_001967"
     CDS             complement(1584..2423)
                     /locus_tag="VYN77_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018369205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEV1559369.1"
                     /translation="MKKVTLQPFTKWTGGKRQLLPVIRELIPKTYNRYFEPFVGGGAL
                     FFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRSADRDE
                     RIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELISAISVY
                     LNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFADQVRLR
                     DAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISEIIVTN
                     YEK"
BASE COUNT          793 a          482 c          294 g          862 t
ORIGIN      
        1 aaatgtttct cgtaaattat tcttagcctt ataccaacct tgtccatcag taatccacat
       61 aaactcaaca ttaggaatag ctttaatttc ttcagcaatc attttgtaag atctagccgt
      121 ctcatttaat tttgaaccac ttcctgaata aaaattaacc tcaatcagat aaagcacttg
      181 ttcatttttt atgacaaaat caaaacgttt cacagtattc ccatcattcg taatagcaga
      241 taaatcgaca gaaaaaattt cctctatttc attttgttca atctctttaa ataaattctc
      301 tcctaaaata tacccttcag cttcaagata actttgaaca atattttcca tcgcatctcc
      361 agtccgattt ttgcgcccat tagtatccat accaacctca acacctgtaa cgtagtcaac
      421 taaatttgat actaaatgat tttgcaataa atcaaatata cctgattttt ccaaaaacat
      481 agtatattct tcaatactat agtttcgttt actaaaatca aaatagaaat cttttatcgg
      541 atccttaaca ataatcgtat ctctcaaacg tttagcaata agtagaggca caactttcaa
      601 aatttctgga taattttgat ataagcctaa aaaatcctct tgtatatttt tactaccaat
      661 aagactattc ataatattta gagcaacttt tatactagat acatttttat aaaccttagg
      721 aaaatctgta taataagtcc agtcagctac tgtgtcggtc atcgttgaca accactcatc
      781 aaaatttcta gtttgtttca ttttctgcct ccaacctctt ccttgcaatt tttaaatatt
      841 ctttttcagc atcgatacct ataaatcttc tacctaaccg cttcgcaaca acacccgtag
      901 tgccactacc aacaaatggg tctagaatat agtcaccctc tttagtagag gctaaaataa
      961 tgcgttctaa caaatactct ggtttttgag ttggatgttt cccagcccat ttttcaactt
     1021 tctttgttaa agaaccggtc cagacatctt tcatttgttt tccatcattc aattctttca
     1081 ttaaatcata attgtagtaa tgacgagctt ttttatcatt ttttctggcc cataaaatgg
     1141 tttcagtaga atgggtaaaa taacgacaag ataaattggg ggcagggttt gttttctgcc
     1201 aagtaatatt attcagaatt ttaaaacctt cttgttctaa tgccattcca actgagtata
     1261 tgttgtgcaa actacctgaa atccataccg tcccattagg cttcagaact tcttttgcta
     1321 ggcgaatcca tttacgatta aactcatgtt tttcttcgaa tgaagaaatt ttatcccaat
     1381 ctcctttatc aacagaaaca acttgtcccc cagaattaga tattccacca ttacttaaaa
     1441 aataaggtgg atcagcaaaa atcatatcca tactttctgg tttcattttt gataagaact
     1501 tgaacgtatc tgaatgaaca agaatcatct tatttttatt gtagtatggt tttgtcataa
     1561 gaacacctcc atacttatat tcgttatttt tcataatttg tgactataat ttcagaaatt
     1621 tttcctcgac ttgaagattt tgctccatta gttcgggtag cttcaacata atgtatatta
     1681 aaatccttat acaactcctc tactaaagca ctagaagaat ttgataacat aacataagct
     1741 cctgtatcac tcaatctctt aaaggcatct cttaatctta cttgatctgc aaaagagaat
     1801 ccctcatgag tataagacgt aaatgcactc gtctcagaca atggaatata tggagggtca
     1861 aaatacacaa aatctcctgt tcgaacatct acaattgcct tttcaaaatc tcccacttta
     1921 atttctagtt gattgttatt tagataaact gaaatagcag atatcaattc ctcatcaaca
     1981 attttaggat tcttataacg tccatatgga acattaaatt ggttcttaga attcacacga
     2041 tatagaccat taaagttcac tctcaacata tatagaatac gtgcagctct ttgtacttcg
     2101 gacatcatat ctattctttc atcacgatct gcagaacgta aatctaaata atattctttt
     2161 gaattatatt cctgatgaac tttcaaaatt tcaatcaatt cttgaggatt gtccttaatt
     2221 tgttgatagc aatttattag ttcagcgtta aaatcattaa taactgcatc tttaggagcc
     2281 aaatcaaaaa ataaagctcc acctccaaca aaaggttcga aatacctgtt ataggtttta
     2341 ggtattaatt ctctaataac aggcaataat tgtcttttac cacctgtcca tttcgtgaac
     2401 ggttgtaaag taactttctt tatttctttt a
//
LOCUS       DAVLVZ010000117         2421 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000117 DAVLVZ010000000
VERSION     DAVLVZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.117"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..255
                     /locus_tag="VYN77_001968"
     CDS             <1..255
                     /locus_tag="VYN77_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559370.1"
                     /translation="GFLGIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQ
                     IVVINEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
     gene            569..1546
                     /locus_tag="VYN77_001969"
     CDS             569..1546
                     /locus_tag="VYN77_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559371.1"
                     /translation="MKNKHYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            1923..2297
                     /locus_tag="VYN77_001970"
     CDS             1923..2297
                     /locus_tag="VYN77_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV1559372.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGN
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          818 a          311 c          432 g          860 t
ORIGIN      
        1 ggatttttgg gaatcattta cgataatgaa gcattaagag atttgtcaaa acaatattta
       61 aacaaaaata tttcggaatt acaagatggt tctttcgagg gcggtataca agatgctgtt
      121 gcctctaaac aaatagtagt tataaatgaa tttatagctt gtttaaattc cagatgcgtg
      181 ccatcccaat atgatagatt tttttataaa gatagacctt atattttttc cagaaagagt
      241 cctattaagg ggtgaaaatt tgttacattt ggattaacaa gtgtcctagg gattagaaat
      301 aactctagta tagctagata tttttgagta actagaaata tcattagtct acccatatga
      361 ttgccagaaa agatggctga tgaatcttat ttatactgtt gcagtaggcg ttgtctagga
      421 taaataagct atcttgatgg gggagattgg aataatttta gtagaaacat taaaggatta
      481 tggacaagta gcgcaggatt aggtgatgca atgtgctaag ttggcttggt gttagcagta
      541 gtattattta acaatggaaa gtagactaat gaaaaataag cattattttt ttgacacaat
      601 tttaataatt ttactgctta taagcacgat attttgtgtc agcccagtat ttataaaatt
      661 ggacatctta ggaactccct ctcatgccat cttaaccttt gtattggcga taccgttatt
      721 ctatattttg tctcaatgct tacatacttt gctattacta gtttcctcaa tcttttgtaa
      781 attaagacca atttattttt attttatatt tgtgatcatt ataggagcta ggaaatacta
      841 tcgaattctc tttcaccaac taatgggatt tagtccagga atagcagtgt tttataagga
      901 gagtcaaaca actaagaatc tgtttaaatt ttactacttc ttgtacttta caacactaat
      961 ttcatactat tttttcttta cttttgtgta tgacaaaccg ttgcttttgc ctcttatttc
     1021 attttctatt atcatagcat tagtacaaaa actttataga attgagaatc aacaattatt
     1081 tctgttaaaa tcaaaggtac taacaatact ggaatctaag aagaattgtg agtttaatct
     1141 tcaggattat catgaaattt ggaaattgca atcaaaatct gagttgccat gcgtggcttt
     1201 atcttatatc agtttaatca agccatatct ttcagagagt gtgcgtgaac aaattgattt
     1261 attagaagtt aagcgattta aaaaaataaa tcatccaata tcactttatg gtatgttgga
     1321 tgttataaaa ttaaatctat atttacggca ttataatgaa aaaaataaat atgaatcaat
     1381 gttaaagaaa attttagagg taagaccaga ctttgtttta atcgagcaga atattgatga
     1441 tagtttaaat agctcgcaac cattatcttt atctttagcc atttcagaaa tccaactttt
     1501 gcttgaagtg tatatgggga tcaaacatgt ttcaattagg agataaaacc agaataatat
     1561 ttgccattat aggggcaagt tgcatattag tcagtatgta tcttattcca cttattaaaa
     1621 ctgagaataa attttgggaa atacttttat ttatattagg tgtattgcta attggaattg
     1681 cgttattaag tcaaaaaaaa taaattttga aatagtatta gtgagttcgc aatcatttat
     1741 tgctagtggt ttagaactgt taagtttcaa acaataattg attttaaacc ttaggtaacc
     1801 tataaaaaca aataaggtag agaaaggata tcttatctag gtagctaagt gataaaaaca
     1861 cagtcgtcta tcacttgtta ggaaactatc ctaactatgc ttaataaaaa aggagaaaat
     1921 aaatggaact cgtattacca aataattatg ttgttcttga gcaagaagag atgatgtatc
     1981 ttgatggggg attttctatt ccgagatggc ctgttgcaac agccattaat atagctttta
     2041 atggtgtttt aggtggagga gcaatcagtc tagttagaaa ttatattcgt aattatggtt
     2101 tgcggcgagt tacaagcgca attgctggag cagctgcaag atatgttggg gtacgagttg
     2161 caaatagagt ggcaggattt gcactgtctg ctattaatgg atttgcagct tggatgtcaa
     2221 ttggcaatgc tattacaaca atctgggcca ataatgatgt aaataggaga gacccaaatt
     2281 taaacgcctt gtggtaacta gatatgggta ttaaaatgga aaaaattttt gttattattt
     2341 tttttgtatg tctatttata tcatccataa cttttttagc ctatgatttt gttagcgaag
     2401 aactaaaaaa gttgattatt t
//
LOCUS       DAVLVZ010000118         2420 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000118 DAVLVZ010000000
VERSION     DAVLVZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.118"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..356
                     /locus_tag="VYN77_001971"
     CDS             <1..356
                     /locus_tag="VYN77_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1559373.1"
                     /translation="CPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKYVSYLPFPTPEF
                     FLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQEQRRAVSKIQPE
                     SSMFQCIKEKVEALF"
     gene            736..1539
                     /locus_tag="VYN77_001972"
     CDS             736..1539
                     /locus_tag="VYN77_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV1559374.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1655..2420)
                     /locus_tag="VYN77_001973"
                     /pseudo
     CDS             complement(1655..2420)
                     /locus_tag="VYN77_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          747 a          413 c          447 g          813 t
ORIGIN      
        1 gatgtccaat taaatttaat gtttcaggta ataaagggca aaaaaataat attgaattaa
       61 ttgcgaaaca aagaagcttt atagattatt gggctaaata cgtttcttat cttcctttcc
      121 caaccccaga gtttttttta gctaatttgt gtaacagcgt agatagggaa ggttatgatt
      181 tttctaaaga tggcaacgga aaagaatatt ttagaaaaaa aactcaagta gcgttaggaa
      241 tagagaatat aacttctgaa gatattttcc aggagcaaag aagagctgtt tcaaaaatac
      301 agccagaatc atcaatgttt cagtgtatta aagaaaaagt agaggcattg ttctaattcc
      361 ttagattagg tactacataa caaatttttt aaattcaaac ccgcaccaaa aacaacattg
      421 caactttctt ctcaggtaat gtgttgggat ttgaacagac caacgagttc atagaggtgt
      481 atgccaatga gtttgataag tatgcgcgtc agacttatta gttcatttat ccagatatat
      541 ctcgataatc atattattta gtcaaagaac atcccatatg agggcatgaa atgctaagtt
      601 tagcttgctc actattaaga tgaagtatat atatttcata gaacttattg tgaaggattt
      661 ttataatttt cgccaattcc atacccattt caccatttac cgatgacaca gaaaaacaat
      721 tgaaaggata tttctatgca accaacttac aacattgaca atccaaactt gtcttatgaa
      781 gcgaagcgtg atttatggcg gataggtttt ggtctgcaga aagttgacaa tctagtgcca
      841 tcagcgtata tggaatcttt ggctgagaaa cagtcccggg gagaactgac ttatgagcag
      901 gtttatgagg atgcaacggc ttatcaccat actattgatg caagtacgga agaggcagac
      961 ttggtttctc tacgtattgt agaactattg tctcgaagag gctttagctt tagtcctgcg
     1021 accttacttg ctattcataa ggagttgttt caagatatat ttgaaccctc tattccggtg
     1081 gggcaatttc gtcagaccaa tatcacaaag aatgaacctg ttttgaatgg tgaaagtgtt
     1141 gtgtactctg attactccat gattcaaatg accttggatt atgattttaa tcaggaaaaa
     1201 caagttgcat atgcgacact aactcaggcg gatgtggtta aacaaatcca gcattttatt
     1261 tcaggaatct ggcagattca tccatttcgc gaaggcaaca ctcggacagt aacggtcttt
     1321 ttgattcagt atcttcgtga gtttggtttt gatattgata atataccatt tcagcaacat
     1381 tccaagtatt ttcgtgatgc cttggtatta gataatgcaa agattttaca gcgacgtcct
     1441 gagtttttaa cagctttttt tgaaaatctc ttgctcggtg gtcaaaatga tttgtcttca
     1501 gaaaaaatgt atctagattt agaccttgat ttttcataat ccaaatactg agtaaacatt
     1561 gaattttagg ccaaaatgaa gtaaatattc tcacaagaaa acgtatatca tcaaagtttt
     1621 aggtgtactg atcccaaaca gttagacaat taatttatcc gaaggattta gttctgtatt
     1681 gcacagggat aagtcctttt agttttacct taattcgttt attgttgtag taatcaatat
     1741 agtctacaat agtttgttcc aattgcttaa gcgactgaaa cgacttctca taaccgtaaa
     1801 acatttccga tttcagaatc ccaaagaaag attccatcat accgttgtct tggctgtttc
     1861 ccttgcgtga catagatgct tgaattccct taccctcaag aaactgatga taagaatcgt
     1921 gttgatattg ccagccttgg tcactatgga gaatcgtatt ctcgtagtgc ttctctgtga
     1981 atgcctgttc caacattgtt tgtacttgtt ctaagttggg tgaagttgaa agattatagg
     2041 cgataatttc gctattaaag ccatctaaaa ctggtgataa gtaaagcttt tgagtacttg
     2101 ctggaatggc aaattctgtc acatctgtgt agcactgttc cattgtttta gagccttcaa
     2161 attggccttg aatgagattc tctgccttct tgccaacgtc tcctttatga gaagaatatt
     2221 ttcgtttctg tcgcatttta gcttgtaaat tgagtacttc atcaagtgtt gaactctttt
     2281 atgatttacc agataaccac gatttcttag ttctaaataa atccgacgat aaccataatt
     2341 tcccttgtgt tcgataaaaa tggattgaat ttcagcttta agctcttggt ccttatctgt
     2401 tttatctagc tgtttcaagt
//
LOCUS       DAVLVZ010000119         2382 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000119 DAVLVZ010000000
VERSION     DAVLVZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.119"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..340)
                     /locus_tag="VYN77_001974"
     CDS             complement(<1..340)
                     /locus_tag="VYN77_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559375.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFP"
     gene            complement(354..1067)
                     /locus_tag="VYN77_001975"
     CDS             complement(354..1067)
                     /locus_tag="VYN77_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1559376.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1557..1664)
                     /locus_tag="VYN77_001976"
                     /pseudo
     CDS             complement(1557..1664)
                     /locus_tag="VYN77_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2086..>2382)
                     /locus_tag="VYN77_001977"
     CDS             complement(2086..>2382)
                     /locus_tag="VYN77_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1559377.1"
                     /translation="TPREEKPQSEKPESPKPTEEPEEESPEESEEPQVETEKVKEKLR
                     EAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLALLKENK"
BASE COUNT          749 a          454 c          372 g          807 t
ORIGIN      
        1 gaggaaaatc tcccatacag ccattacgat tcatattgag gctatatttt cgattagtaa
       61 gataattcgg aaatatattt cctattactc taagattatc tacttgtgaa tcccttctcc
      121 caatataatc cccttctttg actaatgatg gagatttcca agtaaaaatt aaatcgccag
      181 agaaattttt cttatcaaaa aacttaaaaa gagattgata aatactattt gctaattgac
      241 aagtgtcaga tgtaaattca taattaacag cttcattaaa atcaacttca gacatttcaa
      301 tgtactcttc aatagtaata ttagcttttt ttgtagtcat tagctaatct cctttaaaat
      361 gtgatctttt caagaatatt tttatctaaa caatccagca agtcttggta agaatagact
      421 tcgtaagtcg gctgggcttg tgtgtgattt tcgaggtgat gaggattata ccagatagtg
      481 tcaatccccg cattattgcc accttgaatg tcggcggtta gagaatctcc aatcatcagc
      541 gtcttttctt tactaaatcc agcaatttgc tggccaatct tttcataaaa gagagcatcc
      601 ggcttttgag tttgcaactg ttctgagata aagacttgat tgaaataagg tactagacca
      661 gattgagcca aacgtcctgt ctgaatggca gtaatgccat ttgtcgcagc atacaagtca
      721 taatcacgct caatgaggct gtccaagaga tcatgagcgc ccgatagtgt ttgtccctgc
      781 tgagcgagat aaaattggta acgctgggca agaaaactac cgtctttttc ctgtccaaaa
      841 tgagaaaata aacgagaaaa gcgcgtgtta accagctctt gtttactgat tttcttcagc
      901 tccaagtctt tccagagagc cttgttcata ggaacgtaat aatctttata agcctgaata
      961 tccgcaactc cttcttcttt tagaagttgg gtcaaagcca catcctcagc agcatcaaaa
     1021 tcaagaagag tgtggtcgag gtcgaagagt agaaatttgt agaacaattt gaggttttcc
     1081 tttctgaaaa ttcattaaga acattatatc ataaagcacc tcatacaatt aactaattta
     1141 atcacttaaa aaaaattcga acactttcta tacaactgac agctcaaatc tttcagaata
     1201 gaacaatact aactatcgag caccccgtct tcataaatat atgtgtaatt ctagacctag
     1261 aattcctata aactaaatgc tttcatactc ttccaagtaa ttgattgcct taaattttaa
     1321 tttttgaagg attctaaagc tagaatagcc ccatcacaat cagttttgat tgattcacaa
     1381 tttagaaaca ctatagtttc actcctgtta aaataaaaag gaactgcata aagcaatccc
     1441 tttctgattt tgaaatcatt tacttaacat tttatagttg agataatcaa tagcttacct
     1501 ataaaaagag ttatagtaaa attccttatt tattgatttc aagctccgct aactgtataa
     1561 gtaatcggaa catctttagc tactttacta atcagttcaa caacaccttc tgcaatgtgt
     1621 ttggaatgtg aaacgataac aagaccaata ccaatacttt ccatcaaacc accccagttt
     1681 ctaaaatagc agtaaagagt aatcctgatg agaatgatcc aggatcaata tgtccaagaa
     1741 accacatgtt cctaagacaa gagctaacag actggccgtc aataatagta ttgttctttt
     1801 tttcatcatt actccttaac tagtgtttaa ctgattaatt agccagtaaa tagtttatct
     1861 ttatttacac tatctgttaa gatatagtaa aatgaaataa gaacaggaca gtcaaatcga
     1921 tttctaacaa tgttttagaa gtagaggtat actattctaa tttcaatcta ctatattttg
     1981 cacattttca taaaaaaaat gagaactaga actcacattc tgctctcatt tttcgttttc
     2041 ccgttctcct atcctgtttt taggagttag aaaatgctgc tacctttact tattctcctt
     2101 taataaagcc aatagttttt cagcttctgc cataatagta ttgttgtcct gggtgccaaa
     2161 tagtaaatta ttttttaatc ctgtgagagt ctctttggca ttggacttga taattggatc
     2221 ctggattttt ccaagtaaat cttcagcctc tctcagtttt tctttaacct tttcagtctc
     2281 gacctgaggt tcttctgatt cctctggtga ttcttcttct ggttcctctg ttggttttgg
     2341 agactctggt ttctcgcttt gcggtttctc ttctcgaggg gt
//
LOCUS       DAVLVZ010000120         2227 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000120 DAVLVZ010000000
VERSION     DAVLVZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.120"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..786
                     /locus_tag="VYN77_001978"
     CDS             <1..786
                     /locus_tag="VYN77_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1559378.1"
                     /translation="LRIEDVFNTKSELIPERVSQDIKFDKKIELKDIWYKYGLFDDYV
                     LKGINVTIKKGETVAIVGESGSGKSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYR
                     KIFGAVLQNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAE
                     EGNNFSGGQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIA
                     HRLDTIKSADKILVMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            810..>2227
                     /locus_tag="VYN77_001979"
     CDS             810..>2227
                     /locus_tag="VYN77_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1559379.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVK"
BASE COUNT          849 a          264 c          413 g          701 t
ORIGIN      
        1 ttaagaatag aggatgtgtt taatactaaa tccgaattaa ttccagaaag agtcagtcaa
       61 gatataaaat ttgataaaaa aatagaatta aaagatattt ggtataaata tggattattt
      121 gatgattatg ttttgaaagg aataaatgtt actattaaaa aaggagaaac tgttgctatt
      181 gttggagaat caggttcagg taagagtaca ttagctaaaa ttttattagg tttattagaa
      241 cctaatattg gttcaataga agttgatgga gtagaaaaag aagaaattgg tcaaacattg
      301 tatagaaaga tttttggagc agtgttacaa aattcaaccc taagttatgg taccttaaga
      361 gagaatttga catttggaca ctttgtttca gatgaagaat taatgacaaa tctaaattca
      421 attggtctta gcaatgtagt taaatcttta cctcttggat tagagacaat catcgctgaa
      481 gaaggtaata acttttctgg agggcagcag caaatgatac ttttagctcg ttgtcttttg
      541 tcgaaacctt cggtagttgt tttggacgaa gcaacaagta gtttagataa tttatctcaa
      601 caaattacaa cttcttactt aagtgaaatc ggtaccacta agattttaat tgcccatcga
      661 ctagatacta tcaagtctgc agataagatc ttagtaatgc ataatggtga aattgtagag
      721 attgggaccc atagagaact tcttgaacta ggaggcattt ataagcaatt gtattcaaat
      781 aattagtttt tgattaaaag ggtaaattta tgaagattat gaaaaaaaaa tattggactt
      841 tagcgatatt attcttttgt ttgttcaata attctgttac tgctcaagaa atacctaaaa
      901 atcttgatgg caatataact cacactcaga ctagcgaaag tttttctgaa tctgatgaaa
      961 aacaggttga ctattctaat aaaaatcaag aagaagtaga ccaaaataaa tttcgtattc
     1021 aaatcgataa gacagaatta tttgtaacaa cagataaaca tttagaaaaa aactgttgta
     1081 aattggaact tgaaccacaa ataaataacg atattgttaa ctctgaaagt aataatttac
     1141 taggcgaaga taatttagat aataaaatta aggaaaatgt ttctcatcta gataatagag
     1201 gaggaaatat agagcatgac aaagataact tagaatcgtc gattgtaaga aaatatgaat
     1261 gggatataga taaagttact ggtggaggcg aaagttataa attatattct aaaagtaatt
     1321 ctaaagtttc aattgctatt ttagattcag gagtcgattt acaaaatact ggattactga
     1381 aaaatctttc aaatcactca aaaaactatg tccccaataa aggatattta ggaaaagagg
     1441 agggagagga aggaataata tcagatattc aagatagatt aggtcatggt acggctgttg
     1501 tagctcaaat tgtaggggat gacaatatta atggagtaaa tcctcacgtt aatattaacg
     1561 tctatagaat atttggtaag tcgtcagcta gtccagattg gattgtaaaa gcaatttttg
     1621 atgctgtaga tgatggcaat gatattatca atcttagtac tggacaatat ttaatgattg
     1681 atggagaata tgaggacgga acaaatgatt ttgaaacatt tttgaagtat aaaaaggcta
     1741 ttgattacgc gaatcaaaaa ggagtaatta tagtagctgc attagggaat gactccctaa
     1801 atgtatcaaa tcagtcagat ttattgaaac ttattagttc acgcaaaaaa gtaagaaaac
     1861 caggattagt agttgatgtt ccaagttatt tctcatctac aatttcggtc ggaggcatag
     1921 atcgcttagg taatttatca gattttagca ataaagggga ttctgatgca atatatgcgc
     1981 ctgcaggctc aacattatct ctttcagaat taggacttaa taactttatt aatgcagaaa
     2041 aatataaaga agattggatt ttttcggcaa cactaggagg atatacgtat ctttatggaa
     2101 actcatttgc tgctcctaaa gtttctggtg cgattgcaat gattattgat aaatacaaat
     2161 taaaagatca gccctataat tatatgtttg taaaaaaaat tctggaagaa acattaccag
     2221 taaaaaa
//
LOCUS       DAVLVZ010000121         2126 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000121 DAVLVZ010000000
VERSION     DAVLVZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.121"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..209
                     /locus_tag="VYN77_001980"
     CDS             <1..209
                     /locus_tag="VYN77_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1559380.1"
                     /translation="RKSSKLRPLIVEDKACNHALSKERSKVENIFAKAKTFKMFSTTY
                     RNHRKRFGLRMNLIAGIINHELGF"
     gene            253..1668
                     /locus_tag="VYN77_001981"
     CDS             253..1668
                     /locus_tag="VYN77_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEV1559381.1"
                     /translation="MKVLKNYAYNLSYQLLVIILPIITTPYVTRVFSSNDLGTYGYFN
                     SIVTYFILLATLGVANYGTKVISGHRKEIEKNFWGIYSLQLGATVFSLTLYCLLCLTL
                     PFMQNPVAYILGLALVSKGLDISWLFQGLEDFRKITVRNITVKFVGVISIFLFVKSAD
                     DLYLYVFLLTIFELLGQLSMWVPAREFIGRPHFDLEYARHHLKPVILLFLPQVAISLY
                     VTLDRTMLGALASTKDVGIYDQALKLVNILLTLVTSLGSVMLPRVSNLLSSGDHKAVN
                     KMHEISFLIYNLVIFPIMAGMLIVNDDFVTFFLGQDFQEARYAIAIMIFRMFFIGWTN
                     IMGIQILIPHNQNKEFMLSTTIPAIVSVGLNLLLLPKFGYIGAAIVSVLTEALVWAIQ
                     LYYIRAYLKDVPIIRTMIKIILASAIMYSVLLVSKTYIHFSPTINVLVFVVLGGIIYL
                     FSVLSLKVVDVIELKQVIRKN"
     gene            1670..>2126
                     /locus_tag="VYN77_001982"
     CDS             1670..>2126
                     /locus_tag="VYN77_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEV1559382.1"
                     /translation="MHKFKDINLDLLKVIACVGVVLLHTEMGGFKETGSWNFSTYLYY
                     LGTYSIPLFFIVNGYLLLGKKEITYSYILQKVKYILITVLSWTFIVWLFKRDFSVNPI
                     KKLIGSLVQKGYFFQFWFFGALILIYICLPVLKQFLNSKRSYLYFLSVLL"
BASE COUNT          627 a          341 c          384 g          774 t
ORIGIN      
        1 cacgtaaatc cagcaaactc agaccgctaa tagttgaaga taaagcctgt aaccatgcgc
       61 tatctaagga gagaagcaag gttgagaata tctttgccaa agcaaaaacg tttaaaatgt
      121 tttcaacaac ctatcgaaat catcgtaaac gcttcggatt acggatgaat ttgattgctg
      181 gtattatcaa tcatgaacta ggattctagt tttgcaggaa gtctaataat taaattggaa
      241 ttgtgaacgt aaatgaaagt acttaaaaac tatgcataca atctatctta tcaattattg
      301 gtgattatac ttccaatcat tacgaccccc tatgtgacac gggtcttttc ttcgaatgat
      361 ctagggacat atggttattt taactccatc gttacttatt ttatcctctt agcaacgcta
      421 ggggttgcca actatgggac caaggtcatt tcagggcatc gcaaagaaat tgaaaaaaac
      481 ttttggggaa tctactctct gcaattaggt gcaacagttt tttctctaac cttgtattgt
      541 cttctttgtc taactcttcc ctttatgcaa aatccagtag cctacattct aggcttggct
      601 ttggtttcta aaggattaga tatatcctgg ctttttcaag ggctagagga ttttcgaaag
      661 attactgttc gaaatatcac agtcaagttc gttggtgtaa tctctatctt cctctttgtc
      721 aaatctgcag atgatctata cctctatgtc tttttgctaa ccatatttga actcttgggg
      781 cagctaagta tgtgggtgcc tgctcgtgag tttattggta gacctcattt tgatttagaa
      841 tatgctagac atcatttgaa accagtcata ttattgttcc ttccgcaagt ggcgatttcc
      901 ttgtatgtta cgctagatcg taccatgctt ggagcgttag cttctacaaa agatgtaggg
      961 atttatgacc aggctttaaa gttggtaaat atccttctga ccttggtaac ttccttggga
     1021 agtgtcatgt tgcctagagt ttcaaatctt ttatcctcag gggatcataa agcagttaac
     1081 aaaatgcatg agatatcatt tttaatttat aatttggtta ttttccccat tatggcagga
     1141 atgctaattg taaatgatga ctttgttacc tttttcctcg ggcaagattt tcaagaggca
     1201 cgttatgcaa tagcaattat gatttttaga atgttcttta ttggttggac caatattatg
     1261 gggattcaaa ttttgatacc tcataatcaa aataaagaat ttatgctgtc aacaacaatt
     1321 cctgctattg ttagtgttgg attaaatctt ctcttgcttc ccaagtttgg atatatagga
     1381 gcagcaattg tttctgtctt aacagaggcg ctagtatggg ctattcaatt gtactatatt
     1441 cgtgcatacc taaaagatgt tccgattatt agaactatga taaagattat tctagcctca
     1501 gctatcatgt atagcgtttt gctagtttct aagacataca tacatttttc cccaactata
     1561 aatgttttgg tatttgtggt gttaggtgga atcatttatc ttttttcagt tttatctcta
     1621 aaagtggtag atgtgataga attaaaacaa gtaattagga aaaattagaa tgcataaatt
     1681 taaagatatt aacttggatt tactaaaagt aattgcatgt gttggagttg ttttactcca
     1741 tacagagatg gggggattta aagagacagg ttcatggaat ttttcgacat atttatacta
     1801 cttaggaacc tattctatcc ctctattttt tatagttaat ggttatttat tgttagggaa
     1861 gaaagagatt acctattcct acatattaca gaaagtaaaa tatattctaa taacagtgct
     1921 gtcatggacc tttatagttt ggttatttaa acgggatttt tcagttaatc caataaaaaa
     1981 acttatagga tcattggtgc aaaaaggtta tttctttcag ttttggtttt ttggtgcgct
     2041 tatacttatt tatatatgtc tccccgtttt gaaacaattt cttaattcaa aaagaagtta
     2101 tttatacttt ttatctgtat tgctag
//
LOCUS       DAVLVZ010000122         2121 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000122 DAVLVZ010000000
VERSION     DAVLVZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.122"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            77..484
                     /locus_tag="VYN77_001983"
     CDS             77..484
                     /locus_tag="VYN77_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1559383.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQTDEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            649..948
                     /locus_tag="VYN77_001984"
     CDS             649..948
                     /locus_tag="VYN77_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1559384.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1057..2121)
                     /locus_tag="VYN77_001985"
                     /pseudo
     CDS             complement(1057..2121)
                     /locus_tag="VYN77_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          651 a          418 c          372 g          680 t
ORIGIN      
        1 taataacgat actagataat gaggaaaatg caatggcaaa cccaactctc ggagaaaaaa
       61 agcgaataca gactatattg ctcgctatgg cgtatatgca gttatcccta atcctgaaca
      121 aaaacaaatt gttcttgttc aagaaccaaa tggtgcttgg ttcctaccat gtggagaaat
      181 tgaagcaggt gaaaatcatc aggaagccct aaagcgtgag ttgattgaag agcttggttt
      241 cacagcagaa attggtacct attacggaca aactgacgaa tatttctatt ctcgtcatcg
      301 tgatacctac tactacaatc ctgcctacct ctatgaagca acttctttca aagaagtaca
      361 aaagccacta gaaaacttta atcatattgc ctggttccct attgacgagg ctatccaaaa
      421 ccttaaacgt ggtagccata aatgggccat tgaatcttgg aaaaaacagc ataagattgg
      481 ctaatactct tcgaaaataa aattcaaacc acgtcagcgt cgccttaccg tactcaagta
      541 tagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa atcaagcgac
      601 tttattcaaa aagtgctata atagaattag aaaatcggag gaaatattat gaagcttatc
      661 aatacgacaa actcacactc gcaacttgta aaaagtcaac tagaaagtac cgatgcaacc
      721 cttgttgagg tttattctgc agggaataca gatgttattt ttacccaagc tccgcttcac
      781 tatgaaatcc tcatctcaaa caaacaccgt gctattcgtg agcctgaaat cgaaaccatt
      841 caagaatttt tcttgaaacg taaaattgat aaggcctctg ttgatgaagc caatatcaag
      901 acactctact cagagaaatt aattggaatt tcaatcccaa tcaagtaaga aaattgagta
      961 aaaactcgat ttgagagaaa gtccaataag tctttcaata ataaaacgca tagtagcaag
     1021 agttttgtac tgaccccaaa aagttagaca attaatttat tcgaaggatt tagttctgta
     1081 ttgcacaggg ctaagtcctt ttagttttac cttaattcgt ttattgttgt agtaatcaat
     1141 atagtctaca atggcttgtt ccaattgctt aagcgactga aacgacttct cataaccgta
     1201 aaacatttcc gatttcagaa tcccaaagaa ggactccatc ataccattgt ctgggctgtt
     1261 acccttacgt gacatggatg cttgaattcc cttaccctca agaaactgat gataagaatc
     1321 gtgttgatat tgccagcctt ggtcactatg gagaatcgta ttctcgtagt gcttctctgt
     1381 gaatgcctgt tccaacattg tttgtacttg ttctaagttg ggtgaagttg aaagattata
     1441 ggcgataatt tcgctattaa agccatctaa aactggtgat aagtaaagct tttgagtact
     1501 tgctggaatg gcaaattctg tcacatctgt gtagcacttt tccattgttt tagagccttc
     1561 aaattggcct tgaatgagat tctctgcttt cttaccaacg tctcctttat gagaagaata
     1621 ttttcgtttt tgtcgcattt tagcttgtaa attgagtact tttatcaagc cttgaactct
     1681 tttatgattt accagataac cacgatttct tagttctaaa tgaatacagc gataagcata
     1741 atttcccttg tgttcgataa aaatggattg aatttcagct ttaagctctt ggtccttatc
     1801 tgttttatct agctgtttca agtgatagta gtaggtcgaa cgagctagtt taatggcttt
     1861 tagaagaaga tctaacgaaa actcagtcat taattcttga acaatttctg tctttcttct
     1921 ttctcttttt cctccttcaa tcggagttct cttaactttt ttaggatggc attttccgct
     1981 ctcaggtact ctccctcttg ttttctcaac aatagtatac ccgtttttcc tgtattgtgc
     2041 tagccagtta agaagtatcg tacgacttgg gagaccgtat tcaagagaaa ctctatcttt
     2101 agtccagcct tcatgtcaga c
//
LOCUS       DAVLVZ010000123         2103 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000123 DAVLVZ010000000
VERSION     DAVLVZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.123"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..1931
                     /locus_tag="VYN77_001986"
     rRNA            <1..1931
                     /locus_tag="VYN77_001986"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2008..>2103
                     /gene="rrf"
                     /locus_tag="VYN77_001987"
     rRNA            2008..>2103
                     /gene="rrf"
                     /locus_tag="VYN77_001987"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          590 a          427 c          615 g          471 t
ORIGIN      
        1 gcagtcagac tgcgagtgct aagatccgta gtcgaaaggg aaacagccca gaccaccagc
       61 taaggtccca aaataattgt taagtggaaa aggatgtggg gttgcacaga caactaggat
      121 gttagcttag aagcagctat tcattcaaag agtgcgtaat agctcactag tcgagtgacc
      181 ctgcgccgaa aatgtaccgg ggctaaaaca atttaccgaa gctgtggata cctttatagg
      241 tatggtagga gagcgttcta tgtgtgatga aggtataccg tgaggagtgc tggaacgcat
      301 agaagtgaga atgccggtat gagtagcgaa agacaggtga gaatcctgtc caccgtaaga
      361 ctaaggtttc caggggaagg ctcgtccgcc ctgggttagt cgggacctaa ggagagaccg
      421 aaaggtgtat ccgatggaca acaggttgat attcctgtac tagagtatgt agtgatggag
      481 ggacgcagta ggctaactaa agcagacgat tggaagagtc tgtctaagca gtgaggtgtg
      541 aattgagtca aatgcttaat tctataacat tgagctgtga tggggagcga agtttagtag
      601 cgaagttagt gacgtcacac tgccaagaaa agcttctagc gtttaaacat actctacccg
      661 taccgcaaac cgacacaggt agtcgaggcg agtagcctca ggtgagcgag agaactctcg
      721 ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga cttaaagtca
      781 gccgcagtga ataggcccaa gcaactgttt atcaaaaaca cagctctctg ctaaatcgta
      841 agatgatgta tagggggtga cgcctgcccg gtgctggaag gttaagagga gtgcttagcg
      901 taagcgaagg tatgaattga agccccagta aacggcggcc gtaactataa cggtcctaag
      961 gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaat gatttgggca
     1021 ctgtctcaac gagagactcg gtgaaatttt agtacctgtg aagatgcagg ttacccgcga
     1081 caggacggaa agaccccatg gagctttact gcagtttgat attgagtgtc tgtaccacat
     1141 gtacaggata ggtaggagtc taagagatcg ggacgccagt ttcgaaggag acgctgttgg
     1201 gatactaccc ttgtgttatg gccactctaa cccagatagg tgatccctat cggagacagt
     1261 gtctgacggg cagtttgact ggggcggtcg cctcctaaaa ggtaacggag gcgcccaaag
     1321 gttccctcag aatggttgga aatcattcgc agagtgtaaa ggtataaggg agcttgactg
     1381 cgagagctac aactcgagca gggacgaaag tcgggcttag tgatccggtg gttccgtatg
     1441 gaagggccat cgctcaacgg ataaaagcta ccctggggat aacaggctta tctcccccaa
     1501 gagttcacat cgacggggag gtttggcacc tcgatgtcgg ctcgtcgcat cctggggctg
     1561 tagtcggtcc caagggttgg gctgttcgcc cattaaagcg gcacgcgagc tgggttcaga
     1621 acgtcgtgag acagttcggt ccctatccgt cgcgggcgta ggaaatttga gaggatctgc
     1681 tcctagtacg agaggaccag agtggactta ccgctggtgt accagttgtc ttgccaaagg
     1741 catcgctggg tagctatgta gggaagggat aaacgctgaa agcatctaag tgtgaaaccc
     1801 acctcaagat gagatttccc atgattatat atcagtaaga gccctgagag atgatcaggt
     1861 agataggtta gaagtggaag tgtggcgaca catgtagcgg actaatacta atagctcgag
     1921 gacttatcca aagtaactga gaatatgaaa gcgaacggtt ttcttgaatt gaatagatat
     1981 tcaattttga gtaggtatta ctcagagtta agtgacgata gcctaggaga tacacctgta
     2041 cccataccga acacagaagt taagccctag aacgccggaa gtagttgggg gttgccccct
     2101 gtg
//
LOCUS       DAVLVZ010000124         1995 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000124 DAVLVZ010000000
VERSION     DAVLVZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.124"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            78..1388
                     /locus_tag="VYN77_001988"
     CDS             78..1388
                     /locus_tag="VYN77_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1559385.1"
                     /translation="MIYLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVAITNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            complement(1754..1948)
                     /locus_tag="VYN77_001989"
     CDS             complement(1754..1948)
                     /locus_tag="VYN77_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1559386.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
BASE COUNT          571 a          463 c          323 g          638 t
ORIGIN      
        1 accaatagga aacaggtggt cttttgagcg ccaaaattgc caacctattt ccatattcta
       61 actgaaagga caattcaatg atttatttaa taatgattag cgccattgct ctagccattg
      121 gaattggtta ccgcaccaaa atcaatattg gcctgctggc tattgctttt tcttacctca
      181 tcgcaaccac tctcatggga ttaagcccca aagaacttct tcatttttgg ccaacctcac
      241 tcttttttac catttttagc gtctctctct tttataacgt tgcaataact aacggtactc
      301 ttgatgtttt ggctcaacac attctctacc gcacacgcac ccaccctaac gccctctaca
      361 tgattttata cctgatggca acccttttgt ctgctttagg tgctggattt ttcactacta
      421 tggccgtttg ctgtcctcta gcgattaccc tctgtcaaaa agcggacaaa caccctttga
      481 ttggagctca agccgtcaat tggggagctt caggaggggc taatttgata accagtagct
      541 caggcattgt ctttcaaggc ctgtttaagc aaatgggatg ggaagagcaa gctttttcac
      601 ttggcaatca tatctttatc gtcagcatta tctacccact catcgtcttg cttttattat
      661 cctgttacag ccactatagt aagggaagaa caaactcatc cttgactatt gaccaaccac
      721 cactcctcag caaggttcaa agacaaacca ccttgctcat gatcagcagc atggttttgg
      781 tttggctttt tcccttgttg catctcattt tcccaaacat cgcctggata gctacttatc
      841 gaaaaacatt tgatattggc tttgtctcaa tactcatggt ctgcctcgcc ttaaggctca
      901 aattagggaa gcaagaagcc attttagcta aggttccctg ggctacaatt atcatgctct
      961 gcggcatgag cttactaatg tctctagcgg tcaagtcagg tttagtcaca ctgatagggc
     1021 acctcatgac aacaaccatt cctcatttct ggttacctct gttcttttgt gtgatagccg
     1081 gagtgatgtc actctttagt tccaccctat ccgttgtagc tccagccctc tttcccatca
     1141 ttgctatcat cagtgctcaa aatcctcaga ttgatattca cttactcaca acagccaccg
     1201 ttattggcgc tctatctact aatatctcac ccttttcatc agctggttct ctcattcaac
     1261 tctcacttcc caatatagag gaacgcggcc ttgccttcaa aaagcaaatt attctgggtg
     1321 tccccatcag cttaagtttg ggactcttga ccacctggat tctcatcctt ctagcttcct
     1381 taagttagat tcattatcta acttaagggt ggacttggta taataaagta gacacaaaat
     1441 taagtggaaa cgaggaaaag tcattatgcc aagaaaaacc tttgataaag ccttcaaact
     1501 ctctgctgta aacctcatcc tgtaaaaata attagttcaa ctttaaaaat tcatcccaat
     1561 agtctttatc gatggattca agaatatgaa aaatatgaag aaagtgcgtt cccaggacat
     1621 gggagcgcac ttcgtcatac tcaatttgag atatagtgaa atgaaataag aacaggacaa
     1681 atagatcagg atagtcaaat cgatttctaa caatatttta gaagtagatg tgtactattc
     1741 tagtttcaat ctactatagt aaagttgact ttcctgtacc atttttacca gtaattaatg
     1801 tcacgcctga actcgaagta tcaaaagaaa tattatcaaa caatatacga tgatccaccc
     1861 tatacgaaat attttctact ttcatttttt ctccttttga actaatagca aaagaaacag
     1921 ttctgttttc atactctttc cccttcatct atattattcg taatgaaaaa taaaaaaata
     1981 aaattttttt aaatg
//
LOCUS       DAVLVZ010000125         1973 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000125 DAVLVZ010000000
VERSION     DAVLVZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.125"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..644
                     /locus_tag="VYN77_001990"
     CDS             <1..644
                     /locus_tag="VYN77_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV1559387.1"
                     /translation="ILNTADYGVPQSRKRLVLHGIRKEILDNTNLTLNLPSKTHSNIP
                     ELDLLSWINADIIMDLPPIEGGEEYIGEGIYNHVANKLSNLNIERITHIRNNGGSRTS
                     LPKSLELNCHKNRLGHSDVYGILDINKPSITITGGCMSYSKGRFGHPTQNRALSAREA
                     ARLQSFDDDYIFVGNRGQLAKQIGNAVPVKLAQASGNYFMSVYREIANGQSDN"
     gene            712..>1973
                     /locus_tag="VYN77_001991"
     CDS             712..>1973
                     /locus_tag="VYN77_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1559388.1"
                     /translation="MRFPHYKSLSLNSRINFNFPITLLVGRNGVNKTSILQALYGSPA
                     NKSVSEYWFSTNVDQIDEVDGKQCLIYSYYNVKASRNVEVLKGRSGTKKGLDYWEPYA
                     PQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFFYHYNFKKISTQ
                     RSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEIISEIMDESYTDI
                     QILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVNDIVNAQSNSLIL
                     IDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFPREAVKLLVKNGEK
                     VDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSENLKQNLVVRYI
                     PGGANQIICNNILNSSYLDSDNHYFWLDG"
BASE COUNT          740 a          237 c          340 g          656 t
ORIGIN      
        1 acattttaaa tacagctgat tatggagttc ctcaatctag aaaaagattg gtgttacacg
       61 ggattagaaa agaaattctt gataacacga atttaacttt gaatttgcct tccaaaactc
      121 attcaaatat accagaattg gaccttcttt catggattaa tgcggatata ataatggact
      181 tgcctcctat tgaaggtggt gaagaatata ttggagaagg tatctacaat catgttgcaa
      241 acaaactatc taatttaaat attgagagaa ttactcatat tagaaataat gggggaagta
      301 gaacctcttt gccaaaatct ttagaattaa attgtcataa aaatcgttta ggtcatagtg
      361 acgtatatgg aatattagat atcaacaaac cttccattac tattacagga gggtgtatgt
      421 catattcaaa aggtagattt gggcacccaa ctcaaaatag agctttaagt gcaagagaag
      481 ctgcaagatt acagtctttt gatgatgact atatttttgt tggaaataga ggtcaattag
      541 ctaagcaaat tggtaatgca gttccagtaa aattagctca agctagtgga aattatttta
      601 tgtcagttta tagggagata gcaaatggac aatcagataa ttgagagttt agaaggaata
      661 actaaaaatt tttttaaaaa taaaaatcaa ttttatcctt atattgatta tatacgattt
      721 cctcattaca agagcttatc attaaattca agaataaatt ttaatttccc tattacttta
      781 ttagttggta gaaatggagt aaataaaact tcaattttac aagctttata tggttctcca
      841 gccaataaaa gtgtgtctga atattggttt agtacaaatg ttgatcaaat agatgaagtg
      901 gatgggaaac aatgtttaat ctattcatat tataacgtta aagcttctag aaatgtagaa
      961 gttcttaaag gccgttctgg aacaaaaaaa ggattagatt attgggaacc atatgctcct
     1021 caaaaacaat atgagatgga aagattaccc aaaaataaat atattggaag ttcttcgaca
     1081 gacagatggg atggcattga aaaaaatgtt gtcttttgtg attgtaaaga atatgtttct
     1141 gcttttgatt tatttttcta tcactataat tttaaaaaaa tttctactca acgtagtaaa
     1201 caagatttta taagattacg atctaaacca gttgctgata ttttaaagaa taatacaagt
     1261 agctatacgc gttataaaaa ggaaatggtt atagataatg ttaaagttga tgataaagtt
     1321 tgtgaaatta ttagtgaaat aatggatgaa tcttatacag acattcaaat tttaacacat
     1381 aagttgtatt caaaaggtga tgatataaaa gcttccaaaa ctatttggat gaaaaaatct
     1441 ggaaaagaat attccgaggc ttttgcagga acaggtgagg ctagaattat tttgttagta
     1501 aatgatatag taaatgcgca atcaaattca ttaatattaa tagatgaacc tgaaatctca
     1561 cttcatccga gtgcaatcta taaatttaaa gagtttttac ttcaagagtg tttaaataaa
     1621 aaacatcaaa ttattatcac tacacattct acacaactta taaaagattt tcctagagaa
     1681 gccgtgaaac ttttagtgaa aaacggagaa aaggtagatg ttattgaaaa tattgattat
     1741 caggatgcat tctttgaatt aggtgatgtg tatcattcta ggaagatgat ttatgttgaa
     1801 gatagactag ctaaatatat tctagagttt gttatcactc attcaggtag tgagaatctt
     1861 aaacagaatt tagtagtgag atatattcct ggtggagcaa atcaaataat ttgtaataat
     1921 attttaaact catcgtattt agattccgat aaccattatt tttggcttga tgg
//
LOCUS       DAVLVZ010000126         1896 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000126 DAVLVZ010000000
VERSION     DAVLVZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.126"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(1..1058)
                     /locus_tag="VYN77_001992"
                     /pseudo
     CDS             complement(1..1058)
                     /locus_tag="VYN77_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            complement(1071..1820)
                     /locus_tag="VYN77_001993"
     CDS             complement(1071..1820)
                     /locus_tag="VYN77_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1559389.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
BASE COUNT          656 a          347 c          214 g          679 t
ORIGIN      
        1 tttcaaaaat tacgttatct atactaacgt ttaaatttgg ttcatatact aaaattttta
       61 taccgacaat caatagttca ttaattatac ttaaaatagc tgactctttg taattatctg
      121 aattatattt catccccaat ttatatattc ctactatact ttggctttcg ttccaatatt
      181 tgtttaacta tgaactgttt tctatttgtg tttgaaatat caatcgcttc tatcactggg
      241 gcatttattt ctataaattc tttttttaat tgtttagtat ctttgggaag acaatatcct
      301 ccaaatccaa aagaaggatt attataaaaa tttccaattc ttggatctaa acaaacacct
      361 tttattacaa cttcagcatt taaacttctc ctctcagcaa aagaatctag ttcattaaaa
      421 aaagcaacac ggagagctaa gaatgtgtta gaaaaaagct taattgcttc tgcttcagta
      481 ggagaaacta acataacatt tttaatattg gcagtactat gagtactaat cgaaaggaac
      541 aactctgcaa tttttcttcc ttcaactgtc tcatctccaa caactatgcg acttggatat
      601 aaattatcat atatagaaca accttctctc aaaaattcag ggacaaaaat gatatttttt
      661 gtatcaaaca gcctttttaa tttgtttgaa aagccgatcg gaactattga ctttaaaata
      721 atctttccat taggttttac cctcagaatc ttcgataccg tttgttcgat ttcatatgta
      781 ttaaaactac caattttctc atcataatct gtcggaagcg caataatata ataatcaata
      841 ttatttttaa tttcagaaaa tgtatcaaaa aaagtaatat ttaagttatt ctcgaaaaaa
      901 aacttcataa gctcttcatt tttagatgga agaatgccct tttttaaatt atttattttt
      961 acagaatcta tatcatatgc aacaacttta tatttagatg caaatagtaa cgcgtaggcc
     1021 agcccaacat gccccaaacc aattactgct atattcataa aactacttcc ttatttctta
     1081 atccaaaatc taatagaata agctgcccca ttccttaaat acaactcttt aatattgttt
     1141 aaaagttttt caactgattt ccagattatc aaaatctgag atttatagca caatatcgat
     1201 gatattctat caatataatg tttttcatca agttcctctt gatacatttt taattcttta
     1261 gtttttccca tataactaac catactacta tcacttacat atgggaagtc ctcataatat
     1321 attactttat aacgcataaa ttcaagcgcc cttccaatac tattcacaaa aacatgatca
     1381 acatggtcac caagtgaaag cggacaatat acgacacatt tgtcgtctaa atgcattaac
     1441 agctctttta tgatatcatt ctttaatgtg tcctcatttt ttaattcact atagatatga
     1501 cggtatagaa aattgccatt tctatctttc ctatagagac attcatagta cgataagtgt
     1561 ctaaaatcac attgtagacg ttcacaagct aacctgtctt ctttcttcct ttcttcaatc
     1621 ggatatttcc caaggttaca caacttatga aattgcttag cagagggctg tagctgttgg
     1681 ctcaaagggt aaccagaaaa tatagtaata acaagtacaa tttctccttc tgaagttaat
     1741 tttgaaatat aatcaccaca ggaaaaaatt gcgtcatcta aatgtggaga taaaaagata
     1801 tacttagtat tgttactcat aaccattccc tctacaattt atctaaaaac tcactaagtg
     1861 tctgattaaa ttccacatca tcaaaaaaat tcacct
//
LOCUS       DAVLVZ010000127         1879 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000127 DAVLVZ010000000
VERSION     DAVLVZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.127"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            102..1733
                     /locus_tag="VYN77_001994"
     CDS             102..1733
                     /locus_tag="VYN77_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1559390.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNIIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          544 a          393 c          365 g          577 t
ORIGIN      
        1 attttctttt acaaacttga cgttaacact tgttagattt acttttatcc tttaaaatag
       61 aataggagat attccgcgat tatttttcgg aggaaaaaga aatgtccatt aattggcagg
      121 aaattttatt tcacttttta ggtggtctgg ggctattctt atatagtatc aagaccatgg
      181 gagacggttt gcaacaagct gctggagatc gccttcgttt ttacattgac aaatatacta
      241 gtaatccttt ctttggagtt ctggttggta ttgggatgac tgctctaatt cagtctagtt
      301 ctggtgtaac agttattaca gtcggcctgg tcagtgccgg tctcttaacc ttacgtcagg
      361 ctatcgggat tgtcatgggt gctaatattg ggacaactgt cacatccttt ctcatcggtt
      421 ttaaattagg taactatgcc ctacctatgc tctttatcgg tgccgtctgt ctctttttta
      481 caaaaaatcg gacagtcaat aatatcggac gcatcctctt tggtatcggt ggtatctttt
      541 ttgccctcaa tctcatgagc ggcgcaatgg ctccactcaa ggatttacag gtctttaagg
      601 actatatgat tgagctaagt aagaatcctg ttttgggtgt ctttgtcggt actggcttga
      661 ccttgctaat tcaagcttct tcggctacca ttgggatttt acaaaacctc tacgccggca
      721 atctaattga cctacaggga gctttgccag ttctatttgg tgacaatatc gggacaacca
      781 ttacagccat cattgcctct ttaggggcta atattgcagc taaacgggta gcaggagctc
      841 atgttgcctt caacattatc ggaacagtcg tctgcgttat ttttctagtt ccttttactg
      901 tcctgattca ttggtttgaa gctacgctaa atctagcacc ggaaatgacc atcgcctttg
      961 ctcacggaac ctttaatatt accaacacca ttgtccaatt tccatttatc ggagctctgg
     1021 cttactttgt aaccaagatt attcctggag aggacgaggt tgtcaaatac gaacccttat
     1081 atcttgatga acatttcatc aaacaggccc catctatcgc tctaggaaat gctaagaaag
     1141 agctcttgca cttaggaaac tacgctgcta aagcctttga cctttcctat aagtacatca
     1201 ttgacttgga tgaaaaagtt gctgaaaaag ggcataaaac cgaagaagca attaacacca
     1261 tcgatgagca attaacacgt tatctcattg ccctttcaag cgaagctctc agccaaaaag
     1321 aaagtgaagt gcttaccaat atccttgatt cctcccgtga tttggaacgg attggagacc
     1381 acacggaggc tctactcaat ctgactgact atcttcaacg gaaaaatgtt gaattttctg
     1441 atgccgcctt gaaagaatta gaggaagttt accgccaaac tagtgacttt atcaaagatg
     1501 ctctggatag tgtggaaaac aatgatattg aaaaagcacg cagtcttgta gaacgtcatg
     1561 aagcaatcaa taagatagaa cgtgttctca gaaaaaccca catcaaacgc ctcaacaaag
     1621 gcgaatgttc aacacaagct ggagtcaact ttatcgacat catctcacac tacactcgtg
     1681 tatcagacca cgctatgaac cttgctgaaa aggtttttgc agaacaaatc taataaccaa
     1741 gaagctatcc atcataattg gatggcttct tactttttcc taagcaagac taggctgaat
     1801 gaaactgaaa gagtattctg cagatatata atatattgaa actagaatag tacacctcta
     1861 tttctaaaac attgttaga
//
LOCUS       DAVLVZ010000128         1787 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000128 DAVLVZ010000000
VERSION     DAVLVZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.128"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..966)
                     /locus_tag="VYN77_001995"
     CDS             complement(<1..966)
                     /locus_tag="VYN77_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1559391.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFVLLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPY"
     gene            complement(966..1772)
                     /locus_tag="VYN77_001996"
     CDS             complement(966..1772)
                     /locus_tag="VYN77_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1559392.1"
                     /translation="MLAGSSLAISGVLMQSVTRNPIADPGILGINTGASLSVVIGLSF
                     LGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMALSSFVSFL
                     ILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLNALAMGDEMA
                     VGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDIRTMTISS
                     SFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVNN"
BASE COUNT          655 a          323 c          267 g          542 t
ORIGIN      
        1 gtatggtgaa cctaacaagc cagttacaac acctactgga tatctagctg gtaaaatatt
       61 ttgagagaat atgtctgata acaaaactag taaaattcca accaatccag ctaatattgg
      121 gcttcttttc ttgccaatat ttaaggctat gggaccagct aaaaaagata tacaagctat
      181 tggtcctgta attgaagtag aaaaagcagt taaagataca gcgcaaaaaa ttaaaacaag
      241 ccttgaaagc tcgggatttg ctccaagtcc gattgctatt tcttcaccaa gttcaataat
      301 ttctagtctt ttattaaaaa ataaaactaa tatagtagca ataatactta ctattagaac
      361 aagaggtatg tcatctaact ttgtaaaaga taaagagcca ctgagccatc tcataacttc
      421 ttgtaattca tatcttgcta ctttcaacaa taaaaatgag gtgcctgctc ttgtgacagc
      481 ttgaaaacca atgcctaata ttatcagtct tgctgctgaa aaaccatctt ttttagctag
      541 taaaaataat attaaagatg atgttagtcc acaagttatt gaaataattc cagtagttaa
      601 actatttgtt tttaatacca atatgcaaaa gaccgctgca atagatgaag aacttgtgac
      661 accgattata tcaggacttg caagaggatt tcttaacata gtttgaaaga taaatcctgc
      721 caatccaaaa gaccagccag ctataattcc tgctaataat tttggtaatc taatttccat
      781 aatcgaaaaa ctagctccag gaacagtttc actatttaag actttaatca aagttgaaaa
      841 agaataactt tcatctccga taagtaaaac gaaaaatgat agactgatta ttattaataa
      901 aaatagtgag gaaaatagtg ttattctatt ttttcttttt tgaataccta taattaaatt
      961 ttgcattagt tattaacccc tctatttttc atagttacat aaataagtac tggacccccg
     1021 attattgcag taattatccc tacttcaatt tcacctggtt tacctaacat acggccgatt
     1081 atatcacata taagcaagag ctctgcacct ataaaagatg aagaaatggt cattgtgcgt
     1141 atatctttgc ttataaataa gccacaaaag tgaggaacta taagacctac gaagccaata
     1201 ggtccaccaa ttgcagtaat acttgaacat aaaagcacac ttgcaattat tgcaagtgat
     1261 cttatcctat taacattaac tccaagacca acagccattt catcacccat agctaaagcg
     1321 tttaaatctg atgaaataaa tatagctatc aagtgaccta aaattataaa aggtagtagt
     1381 gtagatatag aagataatgt agctgctcca aggctaccta tttgccaaaa tctaaatttg
     1441 tctaagacgt tattattcgg taaaattaaa aaacttacaa aactgcttaa agccatacta
     1501 acacaagttc ctgataaggc aagttttata ggggtaaggc ctgcttttcc gcttacagca
     1561 atcgcgtata caaaaattgc acttactaag ccaccaatga ttgcaaaact tatatggctt
     1621 atgcttgatg aaattcctaa aaaagaaaga ccaattacta cactaagact tgctcctgtg
     1681 tttataccga gtatacctgg atcagctatt gggtttctag taactgattg catcaataca
     1741 ccgcttatgg caagactaga acctgctaaa attgcaaaaa tagttct
//
LOCUS       DAVLVZ010000129         1618 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000129 DAVLVZ010000000
VERSION     DAVLVZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.129"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..757)
                     /locus_tag="VYN77_001997"
     CDS             complement(<1..757)
                     /locus_tag="VYN77_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559393.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIGTVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYT"
     gene            complement(914..1159)
                     /locus_tag="VYN77_001998"
     CDS             complement(914..1159)
                     /locus_tag="VYN77_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559394.1"
                     /translation="MDIYGRKTIAMKHFFAGIGEIHFLLFLLYLSMSASPIIGLVIGY
                     TKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
BASE COUNT          614 a          281 c          201 g          522 t
ORIGIN      
        1 tagtatattt ttctttataa agcaaaggta aaactttata aaaaatatct aagtcaacct
       61 cactctgatt ctgaagcaag taggagtcaa gcaaattttc aaaaatagct aacaatatga
      121 ttggtgtaaa gtttttgtat tcacatgtca agtattctga gtatcgtgca gatgatattt
      181 cttttatata actttttgaa agcaagattt tttcaaattc atcatctatt attaatcttc
      241 tatttccaat ccaagcggta ttaccaccca aataagattg tgcaaatagc aatatgtaag
      301 aaaatatgaa cgtaaatact gaaagaggta gaattctatg cgtaaaaaaa tagacgataa
      361 tggtagccag gagtaaagat atctctcgtg acaatcgaat tcttttcagc atctttttta
      421 acagtccgta aggttgtcct tggcaatatt cttgaactaa attaatcgga atgacatctc
      481 tcaccatttc tggaaagtag agtaagttta taggttgaaa tctccagctc ccatcataag
      541 taaatggata tagaaccaaa tacttaaaag atttttttct tataaactta attatcacaa
      601 tatgaatgac tgttccaatg aagtatatta aatataaaag taaaaccgcc atcaatatac
      661 ttacaaaaat ccccttggaa tcaatcaatg gaaaataaaa tattgcaact agaagcgaat
      721 aaagtcccgc taataaatat ttctgatcat ctttcattca aaacaccata ttatttacta
      781 agaatacatc aatacaatag agcaactctg taagtcaata tttgataaag caaagcttta
      841 accccttccc cagacgttag cttttcttca acccactaca gttgacaagg agcccaaatt
      901 attgataaaa actttagtca tcatcgtctc tataaaaata ttttctatac actcttgata
      961 ataccaaaat taatgccaat atcataccaa gagctataat aatagaattt attagattta
     1021 ttttagtata tccaatcact agtccaataa ttggactagc agacatgctt agatataata
     1081 aaaataacaa aaaatgaatt tcaccaattc ctgcaaaaaa atgtttcata gctatagttt
     1141 tacgcccata aatatccatc aaatttaccg tctacgatat ctaaaccata tgcaatggct
     1201 cctcctaatg agaagacttc agcaacagta gccgcaatat taagagcatt agtaatagtt
     1261 gccatactaa ctccaccaat atatcttgtt atagcactta gttacctaga taagatatcc
     1321 tttagcttgc ttccaaaatt taagtctaca cctataatat gagttcatat tagtactgct
     1381 aaatcctatc tatagtaatg tcaaacacct aaataaagtc atctctccaa ttcattcaag
     1441 aaatgaagaa aggcaaaaaa tctttaaaat aagaaaagtg catattatca gatgttcaaa
     1501 aatcttgata atatgcgtta tatagataga aagatttact taatctcatt ttcaaattga
     1561 gttttttccc agcctcgttt ttaatttata tcttcaatag aatctaatga ccgagcta
//
LOCUS       DAVLVZ010000130         1576 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000130 DAVLVZ010000000
VERSION     DAVLVZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.130"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>1576
                     /locus_tag="VYN77_001999"
     CDS             <1..>1576
                     /locus_tag="VYN77_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1559395.1"
                     /translation="FSVIIVNCLLAVVCKKINSSSTKLLPEFSGLDFSLWQDYIQSTG
                     SIKELWPSQIELGKQGIFSGKSGIVQMPTSSGKTASINLTLRSAFYSNRIDNALIVAP
                     FRALCREIYRDINAHFVDENNVIVSEVFDLPEIPQDFSIFNDGKKRVFILTPEKLLFL
                     LRNHQSFIDEIGLCIFDEAHLFDDPSRGTNFELLLSTVKQIFPKGIQKILISAVIPNS
                     EAINRWFNEDGVIVSNNSIKTTEKRVAFSDLNGSNEQLYFIDPITFEEEFFVPRTVSV
                     SELEQLGKERKQKVFPELTNANDISIYYGTKLINNGGVGIFCGRKDTVNVILRRFIDL
                     NNRNYDLTDFLKNSDRSEVEKIGNLIGQNLGYDSVEYTCSQLGVFSHHSGIPMGIRIA
                     IEYAFSKSKINNVVCTSTLAQGINLPIKYLIISSVYQAGDAIKVRDFQNLIGRAGRAG
                     KYTEGTIILTEPNIYKSPKNKWKKQNYKALLNPINTEGCQSNILSIIQFKSVVPTDYR
                     FAPIKFDFWNLIKERFD"
BASE COUNT          579 a          189 c          286 g          522 t
ORIGIN      
        1 ttttctgtaa ttattgtaaa ttgtctctta gcagttgtat gtaaaaaaat aaatagttca
       61 tcaacgaaat tattacctga attttcagga ttagattttt ctttatggca ggattatata
      121 caatcaacag gaagcataaa ggagctttgg ccatctcaaa tcgaattagg gaagcaagga
      181 attttttcag gtaaatctgg aatcgttcag atgccaacaa gttcgggtaa aacagcctca
      241 atcaatctaa ctttaagatc agcattttat tcaaatagaa ttgataatgc attgattgtt
      301 gcacctttta gagcattatg tagagaaata tatcgagata ttaatgcaca tttcgttgat
      361 gagaataatg tgattgttag tgaagttttt gatttaccgg agattccaca ggatttctca
      421 atttttaatg atggtaaaaa aagagttttt atacttactc cagagaaatt gttgttttta
      481 ttgagaaatc atcaaagttt tattgatgaa ataggacttt gtatatttga tgaagcacat
      541 ctttttgatg atccaagtcg tggaaccaat tttgaattac tgttgtcaac ggttaaacaa
      601 attttcccaa aaggaataca gaagatttta atatctgctg taataccaaa tagtgaagct
      661 ataaatcggt ggtttaacga agatggagta attgtttcta ataattcgat taaaacaaca
      721 gagaaaagag ttgcgtttag tgatttaaac gggagtaatg agcaattata tttcatagat
      781 cctattacat ttgaagaaga attttttgtt ccacgtactg ttagtgttag tgaattagag
      841 cagcttggaa aagaacgcaa acaaaaagtt tttccagaac tgacaaacgc aaatgatata
      901 agtatttatt atgggactaa gctaattaat aatggtggag taggaatttt ttgtggaaga
      961 aaagacacgg tcaatgttat tttacgaaga tttatagact taaataatag aaattatgat
     1021 ctaactgatt tcttaaaaaa ctcagatagg tctgaagttg aaaaaatagg aaatttaatt
     1081 ggtcaaaatt taggatatga tagtgttgaa tatacatgta gtcagctagg agttttttca
     1141 catcattcag gtatacctat ggggatacgc attgctatag agtatgcttt ttcaaaaagt
     1201 aaaataaata atgtagtatg tacttcaacc ttagcacaag gtataaactt acctattaaa
     1261 tacttgatta tctcaagtgt gtatcaagca ggagatgcaa taaaagttcg tgattttcag
     1321 aatttgatag gtagagcagg aagggctgga aaatatacag aaggaacaat aattttaaca
     1381 gagcccaata tttataaatc accaaaaaat aagtggaaaa aacaaaacta taaagcattg
     1441 ttaaatccta ttaatacaga gggttgtcaa agtaatattc tcagtattat acagtttaaa
     1501 tcagtagttc caactgatta tcgattcgct cctataaaat ttgatttttg gaatttgatt
     1561 aaagagagat ttgaca
//
LOCUS       DAVLVZ010000131         1490 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000131 DAVLVZ010000000
VERSION     DAVLVZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.131"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            29..1375
                     /gene="gdhA"
                     /locus_tag="VYN77_002000"
     CDS             29..1375
                     /gene="gdhA"
                     /locus_tag="VYN77_002000"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1559396.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV"
BASE COUNT          450 a          303 c          317 g          420 t
ORIGIN      
        1 taaaatatga caataaaagg agaacgatat gacatctgct aaagaatata tccaaagcgt
       61 gtttgaaact gtaaaagctc gtaacgggca cgaggctgaa ttcctccaag ctgttgaaga
      121 atttttcaac actttggaac ctgtatttga aaaacaccct gagtatatcg aagaaaatat
      181 cttggcacgt attactgagc ctgagcgcgt gatttctttc cgtgttcctt gggttgaccg
      241 tgatggaaaa gttcaagtaa accgcggcta ccgtgttcaa ttcaactcag ctgttggacc
      301 atacaaaggt ggacttcgtt tccacccaac tgtaaaccaa gggatcttga aattcctcgg
      361 atttgaacaa atctttaaaa atgtcttgac tggacttcct atcggtggag gtaaaggtgg
      421 atcagacttc gatcctaaag gtaaaacaga tgctgaagtg atgcgcttct gccaaagctt
      481 catgactgaa ttgcaaaaac acatcggacc atcacttgac gtacctgctg gtgatatcgg
      541 tgttggtgga cgtgaaattg gttaccttta cggtcaatac aaacgtctta accaatttga
      601 tgctggtgtc ttgactggta aacctcttgg atttggtggt agcttgattc gtccagaagc
      661 aactggttac ggtttggttt actatactga agaaatgctc aaagctaacg gtaacagctt
      721 tgctggtaag aaagtcgtta tttcaggttc tggtaacgtt gctcaatacg ctcttcaaaa
      781 agcaaccgaa cttggtgcaa cagttatctc tgtttctgac tcaaacggct atgttatcga
      841 tgaaaacggt atcgacttcg atcttttggt tgatgttaaa gaaaaacgtc gcgctcgttt
      901 gactgagtat gcagctgaga aagcaactgc tacttatcat gaaggttctg tatggactta
      961 cgctggaaac tatgacattg cgcttccatg tgctactcaa aacgaaatca acggtgaagc
     1021 agctaaacgt ttggttgctc aaggcgttat ctgtgtatct gaaggtgcca acatgccaag
     1081 caaccttgat gccatcaaag tttacaaaga aaatggtatc ttctacggac ctgccaaagc
     1141 tgccaacgct ggtggtgtag ccgtttcagc ccttgaaatg agccaaaaca gccttcgcct
     1201 ctcatggact cgtgaagaag ttgatggacg tctcaaagac atcatgacca acatctttaa
     1261 cacagctaaa acaacttcag aaacatatgg tcttgataaa gactaccttg caggagctaa
     1321 cattgctgcc tttgaaaatg tagcaaacgc tatgattgca caaggcgtgg tataaaaaaa
     1381 aattacggaa actgactacc cagacgggta gccagttttt tcaattccta aaatttaaga
     1441 ttctaatacc ctttatgcaa aaatatagaa ttctaccttt ttctttatat
//
LOCUS       DAVLVZ010000132         1415 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000132 DAVLVZ010000000
VERSION     DAVLVZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.132"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            29..>1415
                     /gene="pcpA"
                     /locus_tag="VYN77_002001"
     CDS             29..>1415
                     /gene="pcpA"
                     /locus_tag="VYN77_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1559397.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGV"
BASE COUNT          558 a          195 c          227 g          435 t
ORIGIN      
        1 tagaaaataa atatgtaagg aaatttaaat gaaaaaaact acaatattat cattaactac
       61 agctgcggtt attttagcag catatgtccc taatgaacca atcctagcag atactcctag
      121 ttcggaagta atcaaagaga ctaaagttgg aagtattatt caacaaaata atatcaaata
      181 taaggttcta actgtagaag gtaacatagg aactgttcaa gtgggtaatg gagttactcc
      241 tgtagagttt gaagctggtc aagatggaaa accattcacg attcctacaa aaatcacagt
      301 aggtgataaa gtatttaccg ttactgaagt agctagtcaa gcttttagtt attatccaga
      361 tgaaacaggt agaattgtct actatcctag ctctattact atcccatcaa gcataaaaaa
      421 aatacaaaaa aaaggcttcc atggaagtaa agctaaaact attatttttg acaaaggcag
      481 tcagctggag aaaattgaag atagagcttt tgatttttct gaattagaag agattgaatt
      541 gcctgcatct ctagaatata ttggaacaag tgcattttct tttagtcaaa aattgaaaaa
      601 gctaaccttt tcctcaagtt caaaattaga attaatatca catgaggctt ttgctaattt
      661 atcaaattta gagaaactaa cattaccaaa atcggttaaa acattaggaa gtaatctatt
      721 tagactcact actagcttaa aacatgttga tgttgaagaa ggaaatgaat cgtttgcctc
      781 agttgatggt gttttgtttt caaaagataa aactcaatta atttattatc caagtcaaaa
      841 aaatgacgaa agttataaaa cgcctaagga gacaaaagaa cttgcatcat attcgtttaa
      901 taaaaattct tacttgaaaa aactcgaatt gaatgaaggt ttagaaaaaa tcggtacttt
      961 tgcatttgcg gatgcgatta aacttgaaga aattagctta ccaaatagtt tagaaactat
     1021 tgaacgttta gccttttacg gtaatttaga attaaaagaa cttatattac cagataatgt
     1081 taaaaatttt ggtaaacacg ttatgaacgg tttaccaaaa ttaaaaagtt taacaattgg
     1141 taataatatc aactcattgc cgtccttctt cctaagtggc gtcttagatt cattaaagga
     1201 aattcatatt aagaataaaa gtacagagtt ttctgtgaaa aaagatacat ttgcaattcc
     1261 tgaaactgtt aagttctatg taacatcaga acatataaaa gatgttctta aatcaaattt
     1321 atctactagt aatgatatca ttgttgaaaa agtagataat ataaaacaag aaactgatgt
     1381 agctaaacct aaaaagaatt ctaatcaggg agtag
//
LOCUS       DAVLVZ010000133         1326 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000133 DAVLVZ010000000
VERSION     DAVLVZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.133"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            169..1041
                     /locus_tag="VYN77_002002"
     CDS             169..1041
                     /locus_tag="VYN77_002002"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1559398.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            1172..>1326
                     /locus_tag="VYN77_002003"
     CDS             1172..>1326
                     /locus_tag="VYN77_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1559399.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLKEK
                     TGELNHQV"
BASE COUNT          394 a          229 c          280 g          423 t
ORIGIN      
        1 tcctattggt acttgactat tatacacaaa aaaatacaat tgtcttgaca attgtattga
       61 cacttgttag aattaaaaat gaggtattca ggcttgtgat ttcctatgga agttaatagt
      121 aattgcctct agtgcttaca agtgatatta aaaatagagg acttagtgat gtcaatcatt
      181 tcaactgatt taaccccttt tcaaatagat gatacattga aagcagcctt gcgagaagat
      241 gttcattccg aagattacag taccaatgcc atttttgatc atcatggcca agccaaggtg
      301 tcgctttttg ccaaggaagc tggtgtttta gcgggactaa ccgtttttca aagggttttt
      361 accctatttg atgccgaggt gaccttccag aatcctcatc aatttaagga tggggatcgt
      421 ttgactagtg gcgatttggt tttagaaatc ataggctcgg tgagaagtct cttaacatgt
      481 gaacgcgttg ccttgaattt tttacaacat ttatcaggga ttgcttcgat gacagctgct
      541 tatgtagaag ccttaggcga tgattgcatt aaggtatttg atactcgaaa aactactcct
      601 aatttacgtc tttttgagaa atatgccgtg agagttggcg gtggctataa tcatcgcttt
      661 aatttatcag atgctatcat gctaaaagac aatcacattg cggcagtagg tagtgttcaa
      721 aaggcaattg ctcaagcgcg tgcctatgcc ccttttgtga aaatggtcga ggtggaagtg
      781 gaaagccttg ctgctgccga agaagctgcg gcggcgggtg ctgatattat catgttggat
      841 aatatgtcat tggaacagat tgaacaggcc attaccctaa ttgcaggacg ttctcggatt
      901 gaatgttctg gaaatattga tatgaccact attagccgtt ttcgtggttt agcgattgat
      961 tacgtctcca gtggtagttt aacccatagt gctaagagtc ttgatttttc catgaagggt
     1021 ttaacctacc ttgatgtcta agttgtaaaa taaactaact ttttaaagga tgtctttcct
     1081 ctagaacgag ttttatgtca gatagtttaa acgcctcttc aaatatagta aaatgaacca
     1141 aaaatagtac acaatgtggt ataatcttct tatggcatat tcaatagatt ttcgtaaaaa
     1201 agttctctct tattgtgagc gaacaggtag tataacagaa gcatcacacg ttttccaaat
     1261 ctcacgtaat accatttatg gctggttaaa gctaaaagag aaaacaggag agctaaacca
     1321 ccaagt
//
LOCUS       DAVLVZ010000134         1323 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000134 DAVLVZ010000000
VERSION     DAVLVZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.134"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..124
                     /locus_tag="VYN77_002004"
     CDS             <1..124
                     /locus_tag="VYN77_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV1559400.1"
                     /translation="KNTEYKDQISSTVLPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            90..404
                     /locus_tag="VYN77_002005"
     CDS             90..404
                     /locus_tag="VYN77_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559401.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            1088..1249
                     /locus_tag="VYN77_002006"
     CDS             1088..1249
                     /locus_tag="VYN77_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559402.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
BASE COUNT          563 a          164 c          239 g          357 t
ORIGIN      
        1 aaaaaatact gaatataaag atcaaatatc tagcacagta ttgccagata tcaaaattaa
       61 attaccgata gacagtagag gtactccaga ttggaattac atggaaagat atagagatag
      121 ataaagagag agagggagag atagataaaa agtccacccc cattttttat ggagaattca
      181 aaaatgtaag gttaagcaaa gaagaatata aaaatctaaa agaaaaatta aactcacaca
      241 cagatataat gataaataaa ctatccagat acatggaaag cagtggtaag acctatcaaa
      301 accactatgt gacaatctta aaatggtatg aagaagataa agacaaacta agacagaaag
      361 gtttaaataa aaaaatgaat tatgatgtag gagaatcttt atagataaaa agaggtggaa
      421 aggcactatt aaagacttta agtctaaaaa ggtatcaaag tatgccagag ataaaaaaat
      481 ataaatatga cactttaaag gcgattagaa aattaaagaa atgctagaag acttttagat
      541 gacagttacc aagagggtct aattttcact gctacttatg cagtgggaat tggacctttt
      601 tatataagca aattctattt caaaattggt taccaagaaa gacttctttt cactgttaag
      661 tataagaggg taatttatag aattttgtta catcaagata aatattttct gatggtcaaa
      721 ttttaaaaag attaataact ctcgataaat tttgaacctt aacaattgaa tagaccaaga
      781 caagacgtta attatttttg gagcgtaata acctatccgt caagatcttt cggctgaata
      841 attcggcaaa aaaagttctg ctaagcaaaa agaaagtgag cgataagtaa gagtttgaat
      901 atagggaggg ataccctatc cgctatgaag aaaatgagga taatgatact tctaaggttg
      961 aagccggctc atccggaata gaggcggagg tgagattcct atgttgctaa tccaaagcac
     1021 ttgtcaatgt caaaaaactt aattaaacaa ataattatta gatataaaac tttagaggag
     1081 gtatatattg gacaatgact ggaatggatt ggctgattta atagcaaatc tgattgcaaa
     1141 atatgctggt gctttagatt tagataatct acccgatcca acgccagcaa aaaatcaaga
     1201 gatgaaaaat agttttgaca tggcaaaaac acaaattgag acggactaat aaaagtgtta
     1261 taatatactt gaaatgatag tccaaactta aattaaaaaa attatatatg atatagaaaa
     1321 att
//
LOCUS       DAVLVZ010000135         1299 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000135 DAVLVZ010000000
VERSION     DAVLVZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.135"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            122..1132
                     /locus_tag="VYN77_002007"
     CDS             122..1132
                     /locus_tag="VYN77_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin formation protein"
                     /protein_id="HEV1559403.1"
                     /translation="MVINNIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRAGILLFM
                     YYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDYDTNTLA
                     CLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKERVLRLVLSLG
                     DTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQLVPREKIKIH
                     SLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDTSMELES
                     LLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKIVIPSLL
                     EI"
BASE COUNT          467 a          157 c          227 g          448 t
ORIGIN      
        1 ttttccaagc tttgatcagc gaaaaaaagg atataaatta aacttatcta atagaaaaaa
       61 actcccaaat gaaagtttag ttactattct gcataatgaa gtagaaaata ttgcagactc
      121 tttggttatt aataatattt atgacgaata tccaatttgg agtatacctg ttaattttaa
      181 aggttctaaa tggtgtcttg aacaaacggg taatgatatg tataggggta gagctggaat
      241 attactgttt atgtactatg taaaattatt gaaagataaa tcatcatata ctcaactgta
      301 caataaaatg ttatctgcaa ttccgccatc tgtttcttta tctaagacca catttggttt
      361 aactggggat ataggatatt tcattgttct atccattata gaggattacg atacaaatac
      421 tctcgcatgc ttaaattata taaagagtgt tttaactgag cttgaaaaga ctgattttgc
      481 aagtgtttca aacaagtctg actatattaa tggtcttcta cctttaatta atacactggt
      541 aagatattat aggagaggaa tagagaagga acgtgttcta aggctagtat taagtttagg
      601 tgatacgcta tataatgatg taagcgagag agataatagt aattttggat atagttttgg
      661 ccatggacta tcaggtgtaa tttttacatt aaaaaatatt tataaagtga caagacttaa
      721 aaaatataaa gaattaattt tacaactagt tcctagggag aaaatcaaaa ttcattcttt
      781 gaagtggtgt agtggagaat ttgggacatt agttaatcat ccccaaagtg ttccagaggg
      841 aattgtagct agtttagata atgatacatt ttgccatgga agtatggcca taataaattt
      901 ttacatcgac tacatgaaag aaatggatac tagtatggaa ttggagtctt tattgtgtag
      961 tgttgtaatg aataaatcaa aagaaggaga gtatcgtatc gtccaaacaa gcaatttccc
     1021 agatttttct ttgtatacag gtgtgactgg tattgcttat acttttttaa ggtatatcgc
     1081 actgaatcag aatgacaaaa agatagttat cccctcatta cttgaaatct agcttaattt
     1141 atagttttgt ttctaaaata gaggtgtcca ctcattaagc aatataaaaa taccaagatt
     1201 gactagttta atatgtaatt ctataaaata tttttaatag tgtagctttt cgttgagata
     1261 ataatataat aaagaaaagt tctattctaa ttgctaaac
//
LOCUS       DAVLVZ010000136         1295 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000136 DAVLVZ010000000
VERSION     DAVLVZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.136"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            51..791
                     /locus_tag="VYN77_002008"
     CDS             51..791
                     /locus_tag="VYN77_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559404.1"
                     /translation="MENINIKIPEEKISVIIGSNGCGKSTLIKTLSRLIKPLEGEVLL
                     DNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGRFPYRKPFKSLGKDDLEII
                     NRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSILLLDEPTTYLDISYQIEL
                     LDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKLVAEGKPEDILNDKLVKDI
                     FNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT          534 a          200 c          210 g          351 t
ORIGIN      
        1 aaggtctgtg gtcaaataat cttacctgcg gttatgatga aaaaataatc ttggaaaata
       61 taaatataaa aatacctgaa gaaaaaatat cagttattat tgggtcaaat ggttgtggga
      121 aatcaacact cattaaaacc ttgtctcgac ttataaagcc attagaggga gaagtattgc
      181 ttgataataa atcaattaat tcttataaag aaaaagattt agcaaaacac atagctatat
      241 tacctcaatc tccaataatc cctgaatcaa taacagtagc tgatcttgta agccgtggtc
      301 gtttccccta cagaaagcct tttaagagtc ttggaaaaga tgaccttgaa ataataaaca
      361 gatcaatggt taaggccaat gttgaagatc tagcaaataa cctagttgaa gaactttctg
      421 ggggtcaaag gcaaagagta tggatagctc tagccctagc ccaagataca agtatcctac
      481 ttttagatga gccaactact tacttggata tctcatatca aatagaacta ttagacctct
      541 tgactgatct aaaccaaaaa tataagacaa ccatttgcat gattttgcac gatataaatc
      601 taacagcaag atacgctgat tacctatttg caattaaaga aggtaaactt gttgcagagg
      661 gaaagcctga agatatacta aatgataaac tagttaaaga tatctttaat cttgaagcaa
      721 aaattatacg tgaccctatt tccaattcgc ctctaatgat tcctattggc aagcaccatg
      781 ttagctctta ataattgaat attggaattc atttaaaagt cgcaaatttt gaactgaccc
      841 tcaaaagtta ggccaaaaaa ctaactaaag gaggtcagtt ttttaatggc aaatacagca
      901 cagaatttaa gataaaatta gtaaaagaat atcatgaatg aaaaatagga tatagagaat
      961 aacagcaata ttaaaaagat caggactgat aataaacaaa aagaaagtac aacgactagt
     1021 tcaaaagcta aaacttcaag taaaaagtta ttcaagaaaa tctagaaaat actcatccta
     1081 caaaggacag gtaggaaaaa tatcagacaa caaaataaaa agaaatattt attaccgaaa
     1141 aaaaggggaa atcgagtcaa gtaccaacag gctcatctat tccaaaaaga tatttactcg
     1201 atggaaaaac tcctagaatt agtgctacaa atattgacaa tggtatctta ggttactacg
     1261 aagatataga tgataaaaat tatagagttt ttgaa
//
LOCUS       DAVLVZ010000137         1172 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000137 DAVLVZ010000000
VERSION     DAVLVZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.137"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..1020
                     /locus_tag="VYN77_002009"
     CDS             <1..1020
                     /locus_tag="VYN77_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1559405.1"
                     /translation="NKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAF
                     GQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELN
                     GKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLW
                     RDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTS
                     KFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGD
                     DKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNA
                     CKNAK"
     gene            1010..>1172
                     /locus_tag="VYN77_002010"
     CDS             1010..>1172
                     /locus_tag="VYN77_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1559406.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIK"
BASE COUNT          455 a          159 c          196 g          362 t
ORIGIN      
        1 aacaaatttt ttctaatagc tattttagct atgtgtatag tttttagtgc ttgttcttct
       61 aattctgtta aaaatgaaga aaatacttct aaagagcatg cgcctgataa aatagtttta
      121 gatcatgctt tcggtcaaac tatattagat aaaaaacctg aaagagttgc aactattgct
      181 tggggaaatc atgatgtagc attagcttta ggaatagttc ctgttggatt ttcaaaagca
      241 aattacggtg taagtgctga taaaggagtt ttaccatgga cagaagaaaa aatcaaagaa
      301 ctaaatggta aagctaacct atttgacgat ttggatggac ttaactttga agcaatatca
      361 aattctaaac cagatgttat cttagcaggt tattctggta taactaaaga agattatgac
      421 actctatcaa aaattgctcc tgtagcagca tacaaatcta aaccttggca aactttatgg
      481 agagatatga ttaaaattga ttcaaaagcc ttaggtatgg aaaaagaagg tgatgagtta
      541 atcaaaaata ctgaagctcg tatatccaaa gaattagaaa aacatccaga aatcaaagga
      601 aaaatcaaag gaaaaaaagt attatttact atgattaatg ctgcagatac atcaaaattc
      661 tggatttata ctagcaaaga tccaagagca aattatttaa cagatttagg tctagttttc
      721 cctgaatcat taaaagaatt tgagagtgaa gatagttttg caaaggaaat ttctgcagaa
      781 gaagcaaata agataaatga tgctgatgta atcataactt atggtgatga taaaactctt
      841 gaagctttac aaaaagatcc tcttttaggt aaaataaatg caattaaaaa tggtgccgtt
      901 gctgtaattc cagataatac accgttagca gcctcatgca ctccaacacc actttcaata
      961 aactatacta ttgaagaata tctaaatctt ttaggaaatg catgcaaaaa tgcgaaataa
     1021 aaaacaaata aacctaggca taatttttgt aatctgccta ggtcttctta ttacaatatt
     1081 tttgtcatta aagcttggaa caaaagaaat taatatcaga gattttttag cagcttttgg
     1141 aatgggtaat acaaatgatg attttattaa at
//
LOCUS       DAVLVZ010000138         1026 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000138 DAVLVZ010000000
VERSION     DAVLVZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.138"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>1026
                     /gene="phtB"
                     /locus_tag="VYN77_002011"
     CDS             <1..>1026
                     /gene="phtB"
                     /locus_tag="VYN77_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV1559407.1"
                     /translation="PAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETA
                     AGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEA
                     LDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKY
                     TTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPS
                     TDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNI
                     KFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNGQAD
                     TNQTEKP"
BASE COUNT          340 a          202 c          226 g          258 t
ORIGIN      
        1 accagctcca agcaatccaa ttgatgagaa attggtcaaa gaagctgttc gaaaagtagg
       61 cgatggttat gtctttgagg agaatggagt ttctcgttat atcccagcca aggatctttc
      121 agcagaaaca gcagcaggca ttgatagcaa actggccaag caggaaagtt tatctcataa
      181 gctaggagct aagaaaactg acctcccatc tagtgataga gaattttaca ataaggctta
      241 tgacttacta gcaagaattc atcaagattt acttgataat aaaggtcgac aagttgattt
      301 tgaggctttg gataacctgt tggaacgact caaggatgtc tcaagtgata aagtcaagtt
      361 agtggatgat attcttgcct tcttagctcc gattcgtcat ccagaacgtt taggaaaacc
      421 aaatgcgcaa attacctaca ctgatgatga gattcaagta gccaagttgg caggcaagta
      481 cacaacagaa gacggttata tctttgatcc tcgtgatata accagtgatg agggggatgc
      541 ctatgtaact ccacatatga cccatagcca ctggattaaa aaagatagtt tgtctgaagc
      601 tgagagagcg gcagcccagg cttatgctaa agagaaaggt ttgacccctc cttcgacaga
      661 ccatcaggat tcaggaaata ctgaggcaaa aggagcagaa gctatctaca accgcgtgaa
      721 agcagctaag aaggtgccac ttgatcgtat gccttacaat cttcaatata ctgtagaagt
      781 caaaaacggt agtttaatca tacctcatta tgaccattac cataacatca aatttgagtg
      841 gtttgacgaa ggcctttatg aggcacctaa ggggtatact cttgaggatc ttttggcgac
      901 tgtcaagtac tatgtcgaac atccaaacga acgtccgcat tcagataatg gttttggtaa
      961 cgctagcgac catgttcaaa gaaacaaaaa tggtcaagct gataccaatc aaacggagaa
     1021 acctca
//
LOCUS       DAVLVZ010000139          910 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000139 DAVLVZ010000000
VERSION     DAVLVZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.139"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>910
                     /locus_tag="VYN77_002012"
     CDS             <1..>910
                     /locus_tag="VYN77_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV1559408.1"
                     /translation="DNRRLIERWKNENKSNREIAGLLGKAPQTIHNEVKRGTTLQQVR
                     KGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQKFSPEMMVNKKQVK
                     VGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGKSIEERPDVINLRLE
                     NGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVNQALTLLLGEHRILS
                     ITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRRWLPKGTKKTTPKEV
                     AFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          317 a          163 c          191 g          239 t
ORIGIN      
        1 gataaccgtc gcttgattga gcggtggaag aatgaaaata agtccaatcg tgaaattgca
       61 ggcttgttag gaaaggcgcc tcaaacgatt cataatgaag tcaaaagagg tacaacttta
      121 caacaagtga gaaaagggct atacaaaaag gtctattctg ccgattacgc acaaactgtt
      181 taccaattca atcgaaaacg gtcggtgaaa aagttaattt taacaaagga aatcagagag
      241 aagatcttac actatcataa gcaaaaattt tcgcctgaaa tgatggttaa caagaagcaa
      301 gtgaaagttg gtatttcaac catctactac tggtttcata atggtcattt aggattgacg
      361 aaggccgaca tgctttatcc cagaaaaagg aaaggtgtca agaagcaagc tagtccgaac
      421 tttaagccgg caggtaaatc tatcgaagaa cgtcctgacg ttattaatct tcgcttggaa
      481 aatggtcatt atgaaattga taccgtccta ctgactaaga taaaaaatta ttgcctgtta
      541 gtcttaaccg accggcggag cagacaccaa attataaggt taattccaaa taaaactgct
      601 gaatctgtca atcaggcgct tacgttacta ttaggggagc atcgtattct gtccattact
      661 gcagataatg gttcggagtt caaacgattg tctgaggtat ttcctgagga acatatctac
      721 tacgcacatg cttactcttc atgggagaga ggttcaaatg aaaatcataa tcgattaatt
      781 cggagatggt tacctaaagg aaccaagaaa acgactccga aagaagtagc ttttatcgaa
      841 aattggatta acaactaccc taaaaaatgc ttggactaca agtcgccaag tgaatttctt
      901 ttgggtggct
//
LOCUS       DAVLVZ010000140          894 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000140 DAVLVZ010000000
VERSION     DAVLVZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.140"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            295..>894
                     /locus_tag="VYN77_002013"
     CDS             295..>894
                     /locus_tag="VYN77_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559409.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLSLIKYYQFTNDNTLKIFIHNSIGEIYHYFLQRDTAKESILDYSF
                     AHGYCGIAYALFAYSKVLEPSMFYNDLHTFHTELKKLLEK"
BASE COUNT          337 a          144 c          116 g          297 t
ORIGIN      
        1 ttatctcatt ttactataat atttgataag ttatcctaaa aatattatta tgttgttgtg
       61 ttatagattg attgaatcta actaaaggat cctattcaat tactagaact atcacatact
      121 caaggtcagc tcacagatga gcaactattt tggttacaat gtctactaaa tttaagtcaa
      181 acaaataatt tagtcaaaat taaaaaaata gaggaacata aatatgatta caaaacagaa
      241 tgtaatagtg ttctacaatt tttactagat aaaactgtaa attctgaagg aaggatcact
      301 tcttcaacag aatttggaaa tttcgtaagt aatttatcat tccaacacgg aatagctgga
      361 ctactgtttc ctctaaataa attgtacccc ccagaactgg attctaaaat actctctatc
      421 atcaagaagg cagtgacaat tagaacgaca catacatatg aatatcaata ctcactgcta
      481 tttggtgatg caggctatct atggttactc ctacatttat tttctatcag taaaaatcaa
      541 tactatctac aattagcaaa cgtcaccgct aaaaaattaa tagagaatta tgatactcta
      601 gaggaaatag actttgcatt gggaaaatct ggtgtcctat tatcattaat aaaatactat
      661 caatttacca atgacaatac tcttaaaatt ttcatccaca atagtatagg ggaaatttat
      721 cattatttcc tacaaagaga tacagccaaa gaaagcattt tagactatag ctttgctcat
      781 ggatattgtg gaattgcata tgctttattt gcctattcta aagtcttaga accttctatg
      841 ttctataatg atctccatac attccatact gaattaaaaa aattattaga aaaa
//
LOCUS       DAVLVZ010000141          885 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000141 DAVLVZ010000000
VERSION     DAVLVZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.141"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..415)
                     /locus_tag="VYN77_002014"
     CDS             complement(<1..415)
                     /locus_tag="VYN77_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559410.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINKFFPESFLTYTVLISVFITELIIFHL
                     IGKDFDTKLTNEYKKISQFRSKVSQSPWIKYLEISS"
     gene            complement(412..765)
                     /locus_tag="VYN77_002015"
     CDS             complement(412..765)
                     /locus_tag="VYN77_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1559411.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKFGGLGGWLIGAAAGVLATAAEKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
BASE COUNT          310 a          170 c          110 g          295 t
ORIGIN      
        1 aactagaaat ctctaaatat tttatccaag gagattgaga caccttgctt ctaaactgac
       61 taattttctt atattcgtta gtcaatttag tatcaaaatc tttcccaatt aaatgaaaaa
      121 ttatcaactc tgtaataaaa acacttatta atacggtata agtcaaaaaa ctttctggga
      181 aaaacttatt gattaaaatg gggaaactga ttaacagaat cgaatttatg gctagacaga
      241 tagatactat tttatttttc acagaatata gaggtagtga tactattgag tagataaaag
      301 ctaaaaaaat aagcaaaaaa tactccgtat catttataat cgccctgaat gataaaaata
      361 tatacaccca acacacgatg atgtgtattt ttcgaatctt atctacatta ctcatcttac
      421 tgttgcagat atgaatccat cccaaggata agggttcccg ttaatatcac aacctctatt
      481 aagaacacca tatccaatac agtaagctat tttttctgct gctgtagcca atacacctgc
      541 tgctgcacca ataagccagc ctccaagacc tccaaactta atatagttta ttagtttttt
      601 ggttaaaact gcagctccag ctaatgctat aaaagttcct gaactcatag ctaaagctgc
      661 gcaaattcct tgacaagcac gcttgcttaa ataagctccc ccatcaagat acatcattac
      721 ttcttcatca atcacaacat aattatttgg taatacaagt tccatttgtt ttctcctcct
      781 tagttgaaat tgtttcattt gttttacaaa taggacgaac ttaaactatc tttttattac
      841 tcagtaataa tttccgagat ttcattttat gatactctct attca
//
LOCUS       DAVLVZ010000142          863 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000142 DAVLVZ010000000
VERSION     DAVLVZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.142"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(126..314)
                     /gene="pneA2"
                     /locus_tag="VYN77_002016"
     CDS             complement(126..314)
                     /gene="pneA2"
                     /locus_tag="VYN77_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEV1559412.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
     gene            complement(321..545)
                     /gene="pneA1"
                     /locus_tag="VYN77_002017"
     CDS             complement(321..545)
                     /gene="pneA1"
                     /locus_tag="VYN77_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEV1559413.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
BASE COUNT          279 a          173 c          104 g          307 t
ORIGIN      
        1 ttatatttca tttacttacc tcaaacctat acgacttact attgttttaa aaaaaacctc
       61 tctcaaaatt attagttacg agagaggaaa ataaagagca acttttattt ttaaaattga
      121 atattttatt ttttcttgtt gtctttgcca caacgagtct gagctgaccc caaatatgat
      181 gcaataaaac ttaatgtagc agaacatata attgttgatc tagggtcagc accatctgtg
      241 ccaccataaa ccaattgcat ttcttcaaga gataattctt tcaaactctt tccgattaca
      301 aaatcatttt tcattttaat ttaccccaaa caatcattat tgtagctaac tagcccccca
      361 ataccagaaa cagctgcact aatgcatact ttcgagctag ctgcagctga tttcaaaata
      421 ataggagtag gcgtccaacg tggctctgca ccatcagatg caccataaat taaactcatt
      481 tcatcagcag acaatgattt taaacttttt ccacaaaatt tttcgtttga attaaagttg
      541 gtcataagta ataactcctc cttataaaaa gttaaataag ttgctctata tttttcgttt
      601 tttcagataa attataattc tctcattctc gtgcaaaata tcgggtttta atccgccttc
      661 ccaacatcaa gaccattgga cttcttttgc gattgcactc gaatctccaa ttgtttattc
      721 ttctgaataa actgttggta aggaattgta ttactaaaca taaaaacacc tagcaatgca
      781 aatgtaacta attttttaat tccacgtttt tttctcatgc aatgaaaccc ctttcttttt
      841 ccttgttttt attctaacag ata
//
LOCUS       DAVLVZ010000143          820 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000143 DAVLVZ010000000
VERSION     DAVLVZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.143"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            144..>820
                     /locus_tag="VYN77_002018"
     CDS             144..>820
                     /locus_tag="VYN77_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEV1559414.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLE"
BASE COUNT          311 a           97 c          152 g          260 t
ORIGIN      
        1 actaaaatca aaattactcc aaaaatagta ttgattttgg tttaatacaa aacatatgta
       61 ataatgtgag tcaacaaaag tctgtttttg taccatacta tcttgaaaaa tctactaaat
      121 tcaaattgag aggatgaatt aaaatgaata aaatatttat atatgcagga gtgcgaaatc
      181 ataactctaa gactttagag tatactaaga gactttcaag tatcatttct agtagaaata
      241 atgttgatat atctttcagg acacctttca attcagagct agaaatatct aattctgatt
      301 cagaagagtt gtttaagaaa ggaattgata ggcaaagtaa tgctgatgat ggaggtgtta
      361 taaaaaaaga gttattagag tctgatatta taattatctc ttctcctgta tatttacaaa
      421 acgtatctgt ggacacaaaa aactttattg aaaggattgg ggggtggagc catctttttc
      481 gattagctgg aaagtttgtt gtaacattag atgttgctga gagtaacggc tctgataatg
      541 tgtcagaata tcttagagat atattttcat atatgggagg acagatatta caccaagttt
      601 caattaccaa ttcactcaaa gatattgcag aggctcagtt gatggaggca acttataaaa
      661 ttgaagatgt acttgaaggg aaaattaagt acaaaacaac ggattatcaa gaaagagctt
      721 accagacatt aaaattaatt ttagaaaatt atgattccga acattttgag aagatgtatt
      781 gggagaaaaa acgccttttt gaagctaata gtttagagga
//
LOCUS       DAVLVZ010000144          788 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000144 DAVLVZ010000000
VERSION     DAVLVZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.144"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            43..>788
                     /locus_tag="VYN77_002019"
     CDS             43..>788
                     /locus_tag="VYN77_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1559415.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPFDFVVSTSL
                     TFFVLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESDLTKTLKDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISKNHNWSE"
BASE COUNT          254 a          111 c          124 g          299 t
ORIGIN      
        1 atttatccaa aattaagaaa atgataagga cactgtaaaa ttatgatacg taaactaatc
       61 atttttttat taatgctccc tctttttgtc ttgtggctag agttgcattt gttagtgagt
      121 aacttgcaat tgaatctaga aattcccttt gattttgtgg ttagtacgag tcttactttt
      181 tttgttttga tattatcaaa aatagtttta gacatactat acgcattgaa agatttgtac
      241 aagaaagaag ctcttattac gatttttcct tttattttta taggcaggaa gaaagtaaat
      301 gtgaggttct cgccctattt ttcattccat cgtaaaagtt tgtcgcctga tgatttaaga
      361 tctaggatta tatggagttt tattcttgaa attgctatta tcctagtatt tattttaaaa
      421 attccttttg caatcatcat gcttactaca atatttttct ggactataat ggatatcaat
      481 catctcgttt ttaataagac ggaatttctt tttaatcaaa acaaatggca aaaagaagat
      541 tcgtttgaaa gtgatttgac gaaaacgtta aaggataaga tacaaaaatc agaattaagc
      601 tattctgact taatgtccct tcttctctat gatgcgatga accaatccac ctttttaacg
      661 gatagtgaac tttttgagga tattttgaaa aaaatcgaag attctcataa cacccttctg
      721 tgtacaggtc ttgtcgagtt actgctgtat gaaatttcta ttagtaagaa tcataattgg
      781 tcagaaaa
//
LOCUS       DAVLVZ010000145          763 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000145 DAVLVZ010000000
VERSION     DAVLVZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.145"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..581)
                     /locus_tag="VYN77_002020"
     CDS             complement(<1..581)
                     /locus_tag="VYN77_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1559416.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEA"
BASE COUNT          227 a          143 c          123 g          270 t
ORIGIN      
        1 tgagcctctc cctcttcctc gctaaaagta gctagagctc cgttttgaat accattacag
       61 ctaacgaaag ctttagaaaa ttggagatta gagagatttt gtagggtcaa tgtaccaaca
      121 aaagcacctg taatatcgcg ataatttcca cctattaaaa tcaaatctgt taattttcgt
      181 tcgcttaaaa tcagaaaaac aggtagactg ttggttacga cgcggatatt gtcaataggc
      241 aactcacgcg caaaaaactc taatgttgtt cctggtccaa tgaaaatagt ttctctttct
      301 tctactagac tgcctgcaaa atgggctatt tcttgttttt ctgccgtttg gagggcttgt
      361 ttttcaatat ttgatcgctc attagtcaaa agggagttgg ttcgaagttt ttcagctcca
      421 ccatgcacac gaatcagcaa atctttatca gctaattcct gtaaatagcg ccttgcagtc
      481 atatctgaaa cggctatttc gtccataatc tgtttaactg ttatggttcc tttactattt
      541 actatctcta aaattttggt taatttttct tgtttgagca tattatcacc tcgtttccta
      601 ctactatctt accataaact agctcatcat tcaaatacaa aaacaacaaa ataaaacaaa
      661 aacaaaaata tcgaggtttg ttttcaaaac tttcgatatt tttattaagt tattattttg
      721 ttgtttctag tttacttttt gatggttaag agtggtggag aat
//
LOCUS       DAVLVZ010000146          695 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000146 DAVLVZ010000000
VERSION     DAVLVZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.146"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            94..276
                     /locus_tag="VYN77_002021"
     CDS             94..276
                     /locus_tag="VYN77_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV1559417.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            286..651
                     /locus_tag="VYN77_002022"
     CDS             286..651
                     /locus_tag="VYN77_002022"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV1559418.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
BASE COUNT          296 a           84 c          119 g          196 t
ORIGIN      
        1 tagaaatgac cgaattagaa agaaaaaatc gaaaaattag ctaagaaata ttctgataac
       61 ttaaacatca aagttcaaga gagagttcgt gaaatggcaa atgataataa gagccattat
      121 ttgatataca gagttttagg tatttcattt gaagaaggag aaaatatcga tttgtatcaa
      181 aataaaggtc gttttttata caaatatgct ggttcatttt tagaagaagc tgcagtacta
      241 tcctttaacg aaaaatttgg tacagaaaat acttaaaaag ttaacattcc taattctgaa
      301 agtacaaaac ctaagacttt tgaaattgat tgtttagtcg gagaaaaaca cgcatacgaa
      361 ataaaatggt gggatgcaac tacagatgga gaccatataa ctaaagaaca cactagaata
      421 aaagttattc ataacaaagg atatatacca attcggttaa tgttctacta tccaaataga
      481 actcaagcta taaaaattca gcaaacttta gaaacattgt ataacggtat tggagggaaa
      541 tattattatg gagattctgc ctgggaacat ttaagagcag tgaccggtat tgatttactt
      601 agtattctaa cagatattgc aaataaaaaa acaggggtaa aatcaaaatg acagtattaa
      661 aaggagataa cttagaaata ttaaaaacta ttgaa
//
LOCUS       DAVLVZ010000147          687 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000147 DAVLVZ010000000
VERSION     DAVLVZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.147"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..>687)
                     /locus_tag="VYN77_002023"
     CDS             complement(<1..>687)
                     /locus_tag="VYN77_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEV1559419.1"
                     /translation="FENLISYGDMPVIIDFETMLQQPLFDDKTGQSLLDTLFHRVTRT
                     LLLPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKIDFEGGKNL
                     PVRDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTRVLFRDTNS
                     YASVLSFLYHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQMRLLDIPIFYTDTN
                     INEIYDDFGNH"
BASE COUNT          230 a          139 c           74 g          244 t
ORIGIN      
        1 atgatttcca aaatcatcgt agatttcatt gatatttgta tctgtataaa aaatcggaat
       61 atctaataac ctcatttgtt cacattcgct tgctacaata ccttgattag aaaacttatt
      121 gctccagaga ttttccaatg ctttttctct atctaacatt tcttcataaa aatcaggatg
      181 atataaaaaa gatagtactg aagcatagct atttgtgtct ctaaaaagta cccttgtctt
      241 taaaccatac aagtttgctt ttaatagcat tttaaattct tctgttttat ttaactcttc
      301 aaatatcaga taaaaatccc taaaaccttt tttgaaatct tttatatact tatcaaattc
      361 tatatcacca tcccgaacag gcaggttttt cccaccttca aaatcaattt tcccaatatc
      421 aaactttacc ttatcagtat ttaaattaat taaaacttga ccattaaaag catccttctt
      481 aaaatttcct gtaagagcac tcatctcaac atcaagacca tcctcccttt ttactccctc
      541 tgttggaaga agcaaagttc gagtcaccct gtgaaacagg gtatccagca aactctgacc
      601 agttttatca tcaaatagag gttgctgtaa cattgtttca aagtctatta ttacaggcat
      661 atctccatag gaaattaaat tttcaaa
//
LOCUS       DAVLVZ010000148          680 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000148 DAVLVZ010000000
VERSION     DAVLVZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.148"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>680
                     /locus_tag="VYN77_002024"
     CDS             <1..>680
                     /locus_tag="VYN77_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEV1559420.1"
                     /translation="YNAETISCNPKYIYNELKKINRYELVWVLKEPEKYPDVKAVKYM
                     SLKWLYYIVTSQYVISNTGFNFWLPKKKKQIYINTWHGGGAYKDSDNLNNISKAQVKQ
                     IKKSAELEDYFISSCAVFTKEYAKKRVGFKGKILEIGTPRNDFLIRNQADDGLKNSIK
                     NRLGIDDNCSIVIYAPTWRDDASAIEEIDYESLLESLRETYPKVSILVRGHHLQKNLI
                     DIKNSNIV"
BASE COUNT          270 a           73 c          132 g          205 t
ORIGIN      
        1 ttataatgca gagactattt cttgcaatcc aaagtatatt tataacgaac ttaagaaaat
       61 taatcgttat gaattagtgt gggtgttgaa ggagccagaa aaatatcctg atgtaaaagc
      121 agtaaagtat atgtcgttaa aatggttgta ttatatagtt acttctcaat acgtaatatc
      181 aaatacggga tttaactttt ggcttcctaa aaagaaaaaa cagatatata taaatacatg
      241 gcatggaggt ggagcataca aagatagtga caatttaaac aatattagta aagcgcaggt
      301 caagcaaatt aaaaagagtg cagagttgga agattatttt atatcgagtt gtgcagtctt
      361 tacaaaggaa tatgcaaaga aaagagtagg atttaaaggt aaaatactag agatagggac
      421 acctcgaaat gattttttaa ttcgtaatca agcagatgat ggtctaaaaa atagtataaa
      481 aaatcgactt ggaatagatg ataattgttc tatagtaatt tatgctccaa catggcgaga
      541 tgatgcttca gctattgaag agatagatta tgaaagctta ctagaaagtt taagagagac
      601 atatccaaaa gtgagtattt tagtacgtgg tcatcatctt caaaagaatt tgattgatat
      661 aaagaattca aatattgtta
//
LOCUS       DAVLVZ010000149          620 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000149 DAVLVZ010000000
VERSION     DAVLVZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.149"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..611
                     /locus_tag="VYN77_002025"
     CDS             <1..611
                     /locus_tag="VYN77_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1559421.1"
                     /translation="KEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGK
                     STLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGV
                     KNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFIFFDEPSSGM
                     DYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          244 a           70 c          118 g          188 t
ORIGIN      
        1 taaaagaagg tggagagtat cagataaaaa atcttagcta caaatttact gatgatgagt
       61 gtttaagctt aaaagatatt tcattcaagc ttgggaaaat ttatggcata ataggatcca
      121 acggacgagg aaaatcaacg cttttaagat gtttaatagg tcttgagaaa aaatcaaaag
      181 aagaaattta ttttaaggga gagaagctat ctaaaaaaga aagactcaaa aactcttcac
      241 ttgttatgca agatgtaaat catcaattat tcacagatga agtattcaac gagcttagat
      301 taggagtaaa gaattttgat gaagaaaagg cgaaaatcat tttaaaagat ttaggcctgg
      361 acgaatttat tgaaaggcat ccgatgagtt tatcaggagg gcaaaagcaa aggcttgcaa
      421 tagcatctgt tatgtgcaag aattctccat ttatcttttt tgacgaacct tcaagtggta
      481 tggattattc caatatgata aaaatatctg aactgattaa taagtataaa accatggata
      541 aaataatttt tattgtttcc catgatatag aatttttaaa tgaagtggca gatgaaattt
      601 ttgaattgta aaatagagtc
//
LOCUS       DAVLVZ010000150          547 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000150 DAVLVZ010000000
VERSION     DAVLVZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.150"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..471)
                     /locus_tag="VYN77_002026"
     CDS             complement(<1..471)
                     /locus_tag="VYN77_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1559422.1"
                     /translation="MGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLSVLKNL
                     ALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRD
                     IFITKQEFGDIVQEEGLSITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIY"
BASE COUNT          187 a          100 c           63 g          197 t
ORIGIN      
        1 ataaatatct ttaagcgtct cttttttagt ttcaaaatca actactttaa tagagttttc
       61 gttaaaaaat ttaaaaagct cactactttg aatattccca gacatagtaa tgctcagtcc
      121 ctcttcctga acaatatccc caaattcttg tttggtaatg aaaatgtctc tatctgtagc
      181 tgatgaaaaa gctatctcaa acaagaaatt atgactatct tttcctactt tttgaaatgt
      241 caaaagcccg ttctccaaga aaagaaccct ctcacaaatt tcttcaatat cttctaattt
      301 atgactcgat attaaaattc ccacattttc atgtaaagct aaatttttta gaaccgataa
      361 aactatttgt gatgattcaa tatctaaacc attagtcggt tcatctaaaa tcaatatatc
      421 aggttccgta acgagagtta gaagcaaagc taatttttgt tttgtaccca aagaataggt
      481 ctttactttt ttattaatag actgagtcaa atctaactct tggatcaaac ttctaaatat
      541 ttcttga
//
LOCUS       DAVLVZ010000151          524 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000151 DAVLVZ010000000
VERSION     DAVLVZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.151"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(147..>524)
                     /locus_tag="VYN77_002027"
     CDS             complement(147..>524)
                     /locus_tag="VYN77_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1559423.1"
                     /translation="KEHDEVSEPTHPESDEKENHAGLNPSADNLYKPSTDTEETEEEA
                     EDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAMETLTGLKSSLLLGTKDN
                     NTISAEVDSLLALLKESQPTPIQ"
BASE COUNT          141 a          105 c           80 g          198 t
ORIGIN      
        1 ttctaaaccg gttatttaac catttaatta accagtaaat agtttactat ttaaaattac
       61 ttttgtcaag tttctgttac gtttttctaa aaaagagaag taacacgaaa ggttacttct
      121 ccataaaata tgctccattc attttactac tgtataggag tcggttgact ttcttttaac
      181 aaagccaaga gactatctac ttctgctgaa atagtgttat tatctttcgt tccgagaaga
      241 agactacttt ttagaccagt caatgtctcc atagcatttt gtctaatact agaatctgtt
      301 actttttcta gcaaggcctc cgcctctgct atcttagcgt taataacaga atgctctact
      361 tgaggaattt cagcctcatc tgtagtatct tcagcttctt cctctgtctc ttccgtatca
      421 gtgcttggtt tataaagatt atctgctgaa ggatttaaac cagcgtgatt ctctttttca
      481 tcagattcag ggtgagttgg ctcacttact tcatcatgtt cctt
//
LOCUS       DAVLVZ010000152          467 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000152 DAVLVZ010000000
VERSION     DAVLVZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.152"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>467
                     /locus_tag="VYN77_002028"
     CDS             <1..>467
                     /locus_tag="VYN77_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1559424.1"
                     /translation="SHVKNKIDNIESQVYTKTNFINQKDKKGSIEDSVLNIVFVTTRT
                     KYCSQILQLLLKYFDKNPNKISEVYSILEANYGLVTEREYIDYTLAENTISELANLDL
                     TKSYNQELIVTILKQFLKIEIERTETHEEKFTFGRYKVPDSEKLKQYHRSILKL"
BASE COUNT          186 a           57 c           75 g          149 t
ORIGIN      
        1 tcacacgtta aaaataaaat tgataatata gagtctcagg tttatacaaa gactaatttt
       61 attaatcaga aggataaaaa gggatctatt gaggattcag ttttaaatat tgtatttgta
      121 actacacgga caaaatattg tagtcaaatt ctccaattat tgttaaagta ctttgataaa
      181 aatcctaata aaatttctga ggtttatagt attcttgaag caaactacgg gttggttaca
      241 gaaagagaat atattgatta taccttagct gaaaatacca tttctgaact tgctaatctt
      301 gatttgacga aaagttataa ccaagaatta atcgtgacaa ttttgaagca gtttcttaaa
      361 attgaaatcg aaagaacaga gactcatgaa gagaaattta cttttgggcg ctataaagtc
      421 ccggatagtg aaaaattaaa acagtatcat agaagtatac taaaact
//
LOCUS       DAVLVZ010000153          402 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000153 DAVLVZ010000000
VERSION     DAVLVZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.153"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>402
                     /locus_tag="VYN77_002029"
     CDS             <1..>402
                     /locus_tag="VYN77_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1559425.1"
                     /translation="FKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTD
                     RKSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYA
                     HPYASWERGTNENHNRLIRRWLPKGTKKMTP"
BASE COUNT          126 a           85 c           86 g          105 t
ORIGIN      
        1 ctttaaacct gctggtcaat ccatcgaaca gcggtctgaa gctatcaatc ttcgcttgga
       61 gaatgggcat tatgagattg atacggttct acttacgaga gcgaaaaact actgcttgct
      121 tgtcttgacg gatcgaaaga gtagacatca gatcatccga ttgattccaa ataaaagtgc
      181 tgaggtggtc aatcaggctc taaaactcat cttaaaacaa cacaagattc tttccatcac
      241 ggcagataat ggaacggaat tcaatcgctt gtttgatgta ttttctgagg agcacatcta
      301 ttatgcgcac ccctatgcct cttgggaaag gggaactaat gagaatcaca acaggctcat
      361 tcgtagatgg ttacctaagg gaaccaagaa aatgactccc aa
//
LOCUS       DAVLVZ010000154          400 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000154 DAVLVZ010000000
VERSION     DAVLVZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.154"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            186..>400
                     /gene="blpU"
                     /locus_tag="VYN77_002030"
     CDS             186..>400
                     /gene="blpU"
                     /locus_tag="VYN77_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV1559426.1"
                     /translation="MNTKKMSQFEIMDTDMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAA"
BASE COUNT          149 a           48 c           96 g          107 t
ORIGIN      
        1 tagtaaaaaa gtgattagaa aacatctttt ttaaaaatag agatgatttt gaaacaaaaa
       61 agctaattca agacgtttcg atgccaattc aagatttgga tgaaaaaaat taatagatac
      121 tgttatacta aacttgtcaa gtttgtaaca agacaaatat taaaaataaa aaagaggtat
      181 tcgttatgaa tacaaaaaag atgtcacaat ttgaaattat ggatactgat atgcttgctt
      241 gcgttgaagg tggcggatgc aattggggag attttgccaa agcaggtgtt ggaggaggag
      301 cagcacgagg tcttcagcta ggaattaaaa caagaacatg gcaaggtgca gcaactggtg
      361 ctgtgggagg agctatactt ggaggtgtgg cctatgcagc
//
LOCUS       DAVLVZ010000155          386 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000155 DAVLVZ010000000
VERSION     DAVLVZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.155"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
BASE COUNT          128 a           58 c           62 g          138 t
ORIGIN      
        1 gcttattatt ctagtgaaaa attgaatgag ccactattta tttaatatgt atgatgaggg
       61 aatgactcaa aaacttcttg tcgatataag aatatctgag atagtatttt aaaacgaaag
      121 gactataaaa gctcatttcc ttcgcactta ttttatagag atctatcatt atactcttta
      181 gaaatcaaaa agcaacacac cttgtcatcg ttttatcgta aatctgttaa tgagggggct
      241 agtcttatct gcaatctcaa atcagtgttt tgagctgaat ggcactcgtt ttctagtttg
      301 ctctttgatt ttaattgggc cttagtaaat actactctta aattaaacta tagtgaattg
      361 aaataagatg tgaacaactc tatcag
//
LOCUS       DAVLVZ010000156          376 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00064347
            SAMN33607046-rid17938943.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVLVZ010000156 DAVLVZ010000000
VERSION     DAVLVZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607046
            Sequence Read Archive: SRR23725578
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721455.1
            Genome Coverage       :: 220x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:48:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,879
            CDSs (with protein)               :: 1,879
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 55 of 103
            Pseudo Genes (incomplete)         :: 45 of 103
            Pseudo Genes (internal stop)      :: 32 of 103
            Pseudo Genes (multiple problems)  :: 24 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607046-rid17938943.denovo.156"
                     /strain="AUSMDU00064347"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
BASE COUNT          130 a           42 c           67 g          137 t
ORIGIN      
        1 aattttcaat atatagttca acttttaata gcatttgtta ttatttattt ccctttgagt
       61 caactattac agtttttgac acccaatatt attgttcgta gtataggagg ggtggttgtt
      121 tctatactgc tttctatatt atatatgata ggaaggacga aatatgttct acgtaaatag
      181 ttatgttttt gcttataaaa aagaaggtat aatgtattta cgtggtcgga gtatgcggga
      241 aatagctata gaacctcaaa tttcgcaaga atttatcaac gatcaaggaa ctattagaga
      301 tagaagaagt attaggcagt aaactaacat ttgaactatt aaatgaacaa attttaattt
      361 cggatgagat agatat
//
